LOCUS       DASRDG010000001         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_230177, whole genome shotgun sequence.
ACCESSION   DASRDG010000001 DASRDG010000000
VERSION     DASRDG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230177"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFA35_00005"
     CDS             <1..760
                     /locus_tag="VFA35_00005"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZT54577.1"
                     /translation="GVIGLGKVGCLVADAAIKLGMEVHGFDPHITVDAAWSLPAQVRK
                     ATSVADVLKNADFVSLHVPLIDATRDLVNADNLLLMRAGAVLLNFSRDGVANEAAVLD
                     ALDARRLRWYACDFPSPAANAHPQVLALPHLGASTAEAEENCAIMVVDQLRDYLEHGN
                     VVNAVNFPAVSMARESAYRVAIANANVPNMLGQISTAMATAGLNIHNMVNKSRGEMAY
                     TLVDVDSPVAPHVLDAIRAIDGVLSVRDLPDLPA"
     gene            893..>2938
                     /locus_tag="VFA35_00010"
     CDS             893..>2938
                     /locus_tag="VFA35_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZT54578.1"
                     /translation="MANDSDVKSSVPTHQRRVVWILTAAWSLAIFAIAVWWAQDRLHE
                     HHEQSAATAAVRLSGVKDTLSISLRQLAALPLDLSHRRGVPAFVAAGRPADERTPSAD
                     IQPTLDQMAADFDLPLVSLIGGDGEVLASSGRARVPAIGMKLATREYFTDAMRDGASV
                     QFLLGRASRVPGLYFANRVLRDGLPVGVAVVKQDTATLSRLLSDADGARVFVTDANGV
                     VVFANRDDMVMKRVPGLPPLAEEPTRATYLQLPPTLDWEQSHLNDSPRATLVTRVGPM
                     RHATLSSPLAGTALKVWVLEPLDEEAQIARSAWVGGGAIWLLGCLVIWLSWRRIQSLD
                     AALQARRHTHELAQALPLTVFRYTQPAVGEPRFAFLGRGVEELFGMPAANFERDPTLP
                     WRIAGGGDRPPTRPQEFRLESGDTATWIMADSTPTREPDGSTTYNGYWLDITLRRETQ
                     TRFAAVFEHAASGYLFFDPQPGVTHCNPATLRMFGTSDPHVLLGRIPWFPGLSPELQP
                     SGRPSREHALEHLQEHTRSGQRVRSFEWRFRRVDGAVFDTEVHVIALEWAGTPEFCAV
                     VRDITERKQLQAETERARAAAEAASQTKSSFLANMSHELRTPMNAIIGMTHLALEDGL
                     PDKQRDYIEKAHGSARNLLQILNDILDVSKIEAGQMALERIDFELESVVSDMADVLGL
                     "
BASE COUNT          448 a         1078 c         1001 g          411 t
ORIGIN      
        1 gggcgtcatt ggcctgggca aggtcggctg cctcgtcgcc gacgcggcga tcaagctcgg
       61 catggaggtc cacggcttcg acccgcacat cacggtcgac gcggcctgga gcctgccggc
      121 gcaggtgcgc aaggccacga gcgtcgccga cgtgctcaag aacgccgact tcgtgagcct
      181 gcacgtgccg ctgatcgacg cgacccgcga cctcgtcaac gccgacaacc tgctgctgat
      241 gcgcgccggc gcggtgttgc tgaacttctc gcgcgacggc gtggcgaacg aagcggccgt
      301 gctcgacgcg ctcgacgcaa ggcggctgcg ctggtacgcc tgcgacttcc cgagccccgc
      361 ggcgaacgcg cacccgcagg tgctcgcgct gccgcacctg ggcgcctcga ccgccgaggc
      421 cgaagagaac tgcgcgatca tggtcgtcga ccagctgcgc gactacctcg aacacggcaa
      481 cgtcgtcaat gcggtcaact tccccgccgt cagcatggcg cgcgaatcgg cctaccgcgt
      541 cgcgatcgcg aatgcgaacg tgccgaacat gctcggccag atctcgaccg ccatggccac
      601 ggccgggctg aacatccaca acatggtcaa caagtcgcgt ggcgagatgg cctacacgct
      661 ggtcgatgtc gacagcccgg tcgcgccgca cgtgctcgat gcgatccgcg cgatcgacgg
      721 cgtgctgtcg gtgcgcgacc tgccggacct ccccgcctga gcgcctgacc accccgccgc
      781 gcgtgcgcgc gcatcccaca ttcgcgttgc cctgccgccg cacatttctg gtagaagtgt
      841 gcattcctgt gtcttgccga tgccggcaac ccacatgcgt gaggggagac cggtggcaaa
      901 tgacagcgac gtgaagtcga gcgtgccgac ccaccagcgc cgcgtcgtct ggatcctgac
      961 ggcggcgtgg agcctggcga tcttcgcgat cgcggtttgg tgggcacagg atcgcctgca
     1021 cgagcaccac gagcaatcgg ccgcgacggc ggcggtgcgc ctgagcggcg tcaaggacac
     1081 gctctcgatc agcctgcgcc agctcgccgc gctgccgctc gatctgtcgc accgccgcgg
     1141 cgtgccggcc ttcgtcgccg cgggccggcc ggccgacgag cgcacgccgt ccgccgacat
     1201 ccagcccacg ctcgaccaaa tggcggccga cttcgacctg ccgctcgtgt cgctgatcgg
     1261 cggcgacggc gaggtgctcg cgagcagtgg gcgcgcgcgc gtacccgcca tcgggatgaa
     1321 actggcgacg cgcgagtatt tcaccgacgc gatgcgcgac ggcgcgtcgg tgcagttcct
     1381 gctcggccgc gcgagccgcg tgccggggct gtacttcgcg aaccgggtgc tgcgcgacgg
     1441 gctgccggtc ggcgtcgcgg tcgtcaagca ggacaccgcc accttgagcc gcctgctcag
     1501 cgacgccgac ggcgcgcgcg tgttcgtcac cgacgcgaac ggcgtggtcg tgttcgcgaa
     1561 ccgcgacgac atggtgatga agcgcgttcc cggcctgccg ccgctggccg aagagccgac
     1621 gcgcgcgacc tacctgcaac tgccgccaac gctcgactgg gagcagtcgc acctgaacga
     1681 cagcccgcgc gcgaccctgg tcacgcgtgt cggcccgatg cgccacgcga cgctgtcgtc
     1741 gccgctcgcc ggcacggcac tgaaggtctg ggtgctggag ccgctcgacg aggaagccca
     1801 gatcgcgcgc agcgcgtggg tcggcggcgg cgcgatctgg ctgctcggct gcctcgtgat
     1861 ctggctcagc tggcgccgca tccagtcgct cgacgccgcg ctgcaggcgc gccgccacac
     1921 ccacgagctg gcccaggcgc tgccgctcac ggtgttccgc tatacccagc ccgccgtcgg
     1981 cgagccgcgc ttcgcgttcc tcggccgcgg cgtcgaggag ctgttcggca tgccggccgc
     2041 gaacttcgag cgcgacccga cgctgccgtg gcgcatcgcc ggcggcggcg accggccgcc
     2101 gacacggccg caggagttcc gcctcgagag cggcgacacg gccacctgga tcatggccga
     2161 cagcacgccg acgcgcgagc ccgacggcag caccacctac aacggctact ggctcgacat
     2221 cacgctgcgc cgcgagacgc agacccgctt cgccgccgtg ttcgagcacg ccgcaagcgg
     2281 ctacctgttc ttcgatccgc agcccggcgt gacccattgc aacccggcca ccctgaggat
     2341 gttcggcacc agcgacccgc atgtgctgct tgggcgcatc ccgtggttcc cgggcctgtc
     2401 gcccgagctg cagccgagcg gccggccgag ccgcgagcac gcgctcgaac acctgcagga
     2461 gcacacccgc agcggccagc gcgtgcgctc gttcgagtgg cggttccggc gcgtcgacgg
     2521 cgcggtgttc gacaccgagg ttcacgtgat cgcgctggag tgggccggca cgcccgagtt
     2581 ctgcgcggtg gtccgcgaca tcaccgaacg caagcagctg caggccgaga ccgagcgcgc
     2641 gcgcgccgcc gccgaggcgg cgagccagac caagtcgagc tttctggcca acatgtcgca
     2701 cgagctgcgc acgccgatga acgcgatcat cggcatgacc cacctcgcgc tcgaggacgg
     2761 cctgcccgac aagcagcgcg actacatcga gaaggcccac ggctcggcgc gcaacctgct
     2821 gcagatcctg aacgacatcc tcgacgtgtc gaagatcgag gctggccaga tggcgctgga
     2881 gcgcatcgat ttcgagctcg aatcggtggt cagcgacatg gccgacgtgc tcgggctg
//
LOCUS       DASRDG010000002         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_575461, whole genome shotgun sequence.
ACCESSION   DASRDG010000002 DASRDG010000000
VERSION     DASRDG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575461"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..712
                     /locus_tag="VFA35_00015"
     CDS             14..712
                     /locus_tag="VFA35_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54579.1"
                     /translation="MIRIPARFLCILALLAMSPPPPVVSGIAPPDSSPRGASYAQWSA
                     RWWRWLMQHPLAGHPSVDDPSFDVTSGQSGKVWFLATPVSFGTTTPPALTRTITIRPG
                     VALFVGMINGEMSSLEGAATEAEQRAVANFQADRIVGLSATLDGQPVDDILDFRFESP
                     QFTFTAPTPWVFGPTGGTGTSVADGYYLYLEPMGVGQHVLHYTGGFHFEAGVFGPEPF
                     DITADQTYVINVAP"
     gene            complement(734..>2434)
                     /locus_tag="VFA35_00020"
     CDS             complement(734..>2434)
                     /locus_tag="VFA35_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54580.1"
                     /translation="VVDEALQQHAGDWSLLLARATLRHDENDFRRELSNDPAFAQRRA
                     DAFADFADAAAAYQKKVPELLREEESTDVYDHWFYAALGSSELANVTHEQVVDQRQID
                     AVKSALLALPGDAGERHLANFANGLFSRMSTLRPPVKFRYLKAGLAIVGDHKQAFEAR
                     KLFDYYKDLVTEIRLETRVDGSTTVPSGEPFGMFVDLRHTREIERESGGFGRYLRNQN
                     DVNYSYNYGRPTEDYRDKFQQMCKDSLGENFEVLSVTFQTENVHSRATDEYGWRITPY
                     AYVMLKARGPQIDRIPALRLDLDFLDTSGYVVLPVESPALAIETKADAPPRPAADLEI
                     TQILDERQAKDGKLMLEVKATGHGMMPPLAQVLDLQPAGFVVDKTEDTGPQMSRFDPN
                     EQAAIVGERTWNLTLRAKDGLPEVPRTFAFGKPRNAGSKLVLQRYADADLVAATPEVE
                     LLASYGAPSRTGLWLAIAGGLLVIALGSLLFVFRRRAPVEAGGLRLPDQLTPFTVLGL
                     LREIRARDGLDAGSVAELDGAIARVEAGYFSAAAAASQDLRALASDWVARAGRQSVAG
                     "
BASE COUNT          322 a          867 c          856 g          389 t
ORIGIN      
        1 aaggagaaga gtcatgatcc gcattcccgc caggtttctc tgcatcctcg cgctgctcgc
       61 gatgagcccg ccgccgccgg tcgtgtccgg cattgctccg cccgactcga gtccgcgcgg
      121 cgccagctat gcgcagtggt cggcgcgctg gtggcgctgg ctcatgcagc acccgctcgc
      181 gggtcacccc tcggtcgacg acccgtcgtt cgacgtcacg tcggggcaga gcggcaaggt
      241 gtggttcctg gcgacgccgg tgtcgttcgg caccaccacg ccgccggcgc tgacgcgcac
      301 gatcacgatc cgccccggcg tcgcgctgtt cgtggggatg atcaacggcg agatgtcgag
      361 cctcgaaggc gcggcgacgg aggcggaaca gcgcgcggtg gccaacttcc aggccgatcg
      421 gatcgtcggc ctgtcggcca cgctcgacgg acagcccgtc gacgacatcc tcgacttccg
      481 cttcgagtcg ccgcagttca cgttcacggc gcccacgccc tgggtgttcg gtccgacggg
      541 cggcaccggg acctcggtgg ccgacggcta ctacctgtat ctcgagccga tgggcgtcgg
      601 ccagcacgtg ctgcactaca cgggcggctt ccacttcgag gccggcgtgt tcggtcccga
      661 gccgttcgac atcacggccg accagaccta cgtgatcaac gtcgcgccgt agcccgcggt
      721 ctcgccgcgc gtctcagccc gcaaccgact gccggccggc gcgcgccacc cagtcgctgg
      781 ccagcgcgcg caggtcctgg ctcgccgccg cggcggcgct gaagtagccg gcctccacgc
      841 gcgcgatcgc gccgtcgagc tcggcgacgc tgccggcgtc gagcccgtcg cgggcgcgga
      901 tctcgcgcaa gagcccgagc accgtgaacg gcgtcagctg atcgggcagg cgcagcccgc
      961 ccgcttctac gggcgcgcgg cggcggaaca cgaacagcaa cgacccgagc gcgatcacca
     1021 gcaggccgcc cgcgatcgcg agccacagac ccgtccggct cggcgcgccg tagctcgcca
     1081 gcagctcgac ctccggcgtc gcggcgacga ggtcggcgtc ggcgtagcgc tggagcacca
     1141 gcttgctgcc ggcgttgcgc ggcttgccga acgcgaacgt gcgcggcact tcgggcaggc
     1201 cgtccttggc gcgcagcgtg aggttccagg tgcgctcgcc gacgatcgcg gcctgctcgt
     1261 tggggtcgaa ccgcgacatc tgcggcccgg tgtcctcggt cttgtcgacg acgaatcccg
     1321 ccggctgcag gtcgagcacc tgcgcgagcg gcggcatcat gccgtgcccc gtcgccttga
     1381 cctcgagcat cagcttgccg tccttggcct ggcgctcgtc gaggatctgc gtgatctcga
     1441 ggtcggccgc ggggcgcggc ggcgcgtcgg ccttggtctc gatcgccagc gccggcgact
     1501 cgaccggcag cacgacgtag cccgacgtgt cgaggaagtc gaggtcgagc cgcagcgccg
     1561 ggatccggtc gatctgcggc ccgcgcgcct tcagcatcac gtacgcgtat ggcgtgatgc
     1621 gccagccgta ctcgtcggtc gcgcgcgagt gcacgttctc ggtctggaac gtgacggaca
     1681 gcacctcgaa gttctcgccg aggctgtcct tgcacatctg ctggaacttg tcgcggtagt
     1741 cctcggtcgg ccgcccgtag ttgtacgagt agttgacgtc gttctggttg cgcaggtagc
     1801 ggccgaagcc gcccgactcg cgctcgatct cgcgcgtgtg ccggaggtcg acgaacatcc
     1861 cgaacggctc gccgctgggc accgtcgtgc tgccgtcgac gcgcgtctcc agccggatct
     1921 cggtgacgag gtccttgtag tagtcgaaca gcttgcgcgc ctcgaacgcc tgcttgtggt
     1981 cgccgacgat cgcgagcccg gccttgaggt agcggaactt gaccggcggc cgcagcgtgc
     2041 tcatgcgcga gaacagcccg ttggcgaagt tcgccaggtg acgctcgccg gcgtcgccgg
     2101 gcagcgccag cagcgcagac ttgacggcgt cgatctgccg ctggtcgacg acctgctcgt
     2161 gcgtgacgtt cgccagttcg ctgctgccga gcgcggcgta gaaccagtgg tcgtagacgt
     2221 cggtgctctc ctcctcgcgc agcagctccg gcaccttctt ctggtaggcg gcggcggcgt
     2281 cggcgaagtc ggcgaacgcg tcggcgcggc gctgcgcgaa cgcggggtcg ttggacagct
     2341 cgcggcggaa gtcgttctcg tcgtggcgca gcgtcgcgcg cgcgagcagc agcgaccagt
     2401 cgcccgcgtg ctgctgcagc gcctcgtcga cgac
//
LOCUS       DASRDG010000003         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1496312, whole genome shotgun sequence.
ACCESSION   DASRDG010000003 DASRDG010000000
VERSION     DASRDG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496312"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /gene="ftsZ"
                     /locus_tag="VFA35_00025"
     CDS             <1..995
                     /gene="ftsZ"
                     /locus_tag="VFA35_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZT54581.1"
                     /translation="AKPEAGRAAAEEAVDRIRQSIDGAHMLFITAGMGGGTGTGAAPV
                     IARVAREMGILTVGVVTKPFEFEGPRRLKSADAGVAELEANVDSLIVILNDKLLDVLG
                     DDVTQDQAFAHANDVLKNAVGGISDIIHIPGLVNVDFEDVKTVMSEPGKAMMGTAIGS
                     GPDRAAKAAEAAVACPLLEGIDLSGARGVLVLIAANRNTFKLSESRNAMNTIRRYAAE
                     EAHVIYGTAYDESLGDQLRVTVIATGLSPARARAQTPMQVVHSAPMQRTGTDNLPVLN
                     QQVPMPAAQNHDYSGLTTPSVWRNGRTAAAKVDALASNGMDEIEIPAFLRKQAD"
     gene            1063..1998
                     /gene="lpxC"
                     /locus_tag="VFA35_00030"
     CDS             1063..1998
                     /gene="lpxC"
                     /locus_tag="VFA35_00030"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZT54582.1"
                     /translation="MLAQRTLKSMTRAVGVGVHGGQRVEMTLRPAPPDSGIVFRRVDL
                     PRSVAIKVDAFSVCDTRMATTISAGGDPAGPKVKTVEHLLSACSGLGLDNLSVDITAE
                     EVPILDGSAASFVFLLQSAGIVLQDAPKRFLRVKKTVEVKEGEGAALKWARLEPYEGY
                     RLAFEIEFDHPAVSQTGQQFVFEMDPARYKREIARARTFGFTQELDMMRSRGLGRGVS
                     LDNVVAVDEFKVINSDGLRYDDEFVKHKILDAIGDMMMAGRPLLASYTAYKSGHALNN
                     TLVRALLSDPTAYETVTFDDEASAPAGLAELAPAW"
BASE COUNT          360 a          759 c          727 g          321 t
ORIGIN      
        1 gcgccaagcc ggaagcaggc cgcgccgccg ccgaggaagc ggtcgaccgg atccgccagt
       61 cgatcgacgg cgcgcacatg ctgttcatca cagccggcat gggcggcggc accggcaccg
      121 gggctgcgcc ggtgatcgcc cgggtggcgc gcgagatggg catcctcacc gtcggcgtgg
      181 tcaccaagcc gttcgagttc gaggggccgc gccgtctcaa gtcggccgac gccggcgtcg
      241 cggagctcga ggccaacgtc gattcgctga tcgtcatcct caacgacaag ctgctcgacg
      301 tgctcggcga cgacgtcacc caggaccagg ccttcgccca cgccaacgac gtgctgaaga
      361 acgccgtcgg cggcatcagc gacatcatcc acatccctgg cctcgtcaat gtcgacttcg
      421 aggacgtgaa gacggtgatg agcgagccgg gcaaggcgat gatgggcacg gcaatcggct
      481 ccggcccgga ccgcgccgcc aaggcggccg aagcggcggt ggcctgcccg ttgctcgagg
      541 gcatcgacct ctcgggcgca cgcggcgtgc tggtgctgat cgctgccaac cgcaacacct
      601 tcaagctctc cgagagccgc aacgcgatga acacgatccg ccgctacgcc gcggaggaag
      661 cgcacgtcat ctacggcacg gcctacgacg agtcgctcgg cgaccagctg cgcgtgacgg
      721 tgatcgccac cggcctgagc ccggcccggg cgcgcgccca gacgccgatg caggtggtgc
      781 acagcgcgcc gatgcagcgc accggcaccg acaacctgcc ggtcctcaac cagcaggtgc
      841 ccatgccggc ggcgcagaac cacgactact cgggcctgac cacgccgagc gtctggcgca
      901 acggccgcac cgccgcggcc aaggtggatg cgctggcgag caacggcatg gacgagatcg
      961 agatcccggc gttcctgcgc aagcaggctg actgaaaagc ccggccggtg cgaccaagac
     1021 ctcttcgcgg cgcaccggca cggttccagg gacaattgct ccatgttggc gcagcgcacc
     1081 ttgaagtcga tgacccgcgc ggtcggcgtc ggcgtgcacg gcgggcagcg ggtcgagatg
     1141 accctgcggc cggcgccgcc cgattcgggc atcgtctttc gccgcgtcga cctgccgcgc
     1201 tcggtcgcga tcaaggtcga tgccttctcg gtctgcgaca cgcgcatggc gacgacgatc
     1261 tcggccggcg gcgacccggc cgggccgaag gtgaagacgg tcgaacacct gctctcggcc
     1321 tgttccgggc tcggcctcga caacctgagc gtcgacatca ctgccgagga agtgcccatc
     1381 ctcgacggct cggccgcctc gttcgtgttc ctgctgcaga gcgccggcat cgtcctgcag
     1441 gacgcgccca agcgcttcct gcgcgtcaag aagacggtcg aggtgaagga gggggagggc
     1501 gcggcgctga agtgggcgcg gctcgagccc tacgagggct acaggctcgc cttcgagatc
     1561 gagttcgacc atccggcggt gagccagacc gggcagcagt tcgtgttcga gatggatccg
     1621 gcgcgctaca agcgcgagat cgcacgcgcc cgcaccttcg gcttcaccca ggagctggac
     1681 atgatgcgtt cacgcggcct gggccggggc gtgagcctcg acaacgtggt ggcggtcgac
     1741 gagttcaagg tcatcaacag cgacggcctc cgctacgacg acgagttcgt caagcacaag
     1801 atcctcgacg cgatcggcga catgatgatg gccggccggc cgctgctggc ctcctacacc
     1861 gcctacaagt cgggccacgc cctcaacaac acgctggtcc gggcgctgct ctcggacccg
     1921 accgcctacg agaccgtgac cttcgacgac gaggccagcg ctcccgccgg cctggccgag
     1981 ctggcgcctg catggtaagc atccggatga cactgccgat gaacgacgca tgacggtctt
     2041 tcgctggatc atcggcgtct tcagcgggtt cttcgcgctc gccgccatct tcggcttcgt
     2101 tctcttcatc gccttcgatt cccagctctg gctgcagcgg gcgcggcgct ttcgtcacta
     2161 tgtctgg
//
LOCUS       DASRDG010000004         8169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_690903, whole genome shotgun sequence.
ACCESSION   DASRDG010000004 DASRDG010000000
VERSION     DASRDG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690903"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VFA35_00035"
     CDS             <1..174
                     /locus_tag="VFA35_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT54583.1"
                     /translation="ATTVLLVEQNFAMAKALGDTVAVMDDGRVVHRGEMAALAEDEGL
                     QHKLLGLSLATHQ"
     gene            185..1225
                     /locus_tag="VFA35_00040"
     CDS             185..1225
                     /locus_tag="VFA35_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZT54584.1"
                     /translation="MSELPAVPGSGVAPAAAEALPQTRFDFLPLILVAVIALAAWPAV
                     GSSSTWLTLTFAGLAMGMIVFIAASGLTLVFGLMDVLNFGHSLFIALGAYIATSVFAA
                     TVGWTSVASLAPTLAAVGLAMLAAMVLGGVFGLGFERVIVRPVYGQHLKQILVTIGGA
                     IVGEELIKVVWGPAQIPLPPPEALRGSIVIGEATVDKLRLVSVAIGLVVLAALLWTLN
                     RTKIGLLIRAGVQDREMVESLGYRIRRLFVGVFAAGSALAGLGGSLWGMNQQIVVPQI
                     GGQVNVLLFIVIIIGGLGSTLGCLVGALVVGLLTNYVGFLAPKLTMFSSIGLMVAVLI
                     WRPQGLYPVTSR"
     gene            1229..2296
                     /locus_tag="VFA35_00045"
     CDS             1229..2296
                     /locus_tag="VFA35_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZT54585.1"
                     /translation="MLDRLVSHDRPRNRWLALVLLLIVVGLALTPFIFPGTKALNVAA
                     KVIVFVVLAASFDLLLGYTGIVSFAHTMFFGIGAYGIAIATTRLGPTWGALASGALVA
                     LVLSALLSFVIGLFSLRVKAIFFAMITLAVASAFLALASQLSAFTGGEDGLSFKVPVA
                     LQPGTEYLAEPLLGASIDGRFVTYYGLFFAALGLVLMLLRIVNSPFGRVLQAIRENDF
                     RAEAIGYRTVVYRTLASVLSALFATLAGVLLALWLRYNGPDTTLSFEIMLDVLLIVVI
                     GGMGTVYGAVIGSVLLVLAQNYLQDLMKLGASAVEGIPVVSQLVSPDRWLLWLGILFV
                     LSVYHFPSGVVGRLRASARRP"
     gene            2340..4529
                     /locus_tag="VFA35_00050"
     CDS             2340..4529
                     /locus_tag="VFA35_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HZT54586.1"
                     /translation="MKTIQRSYLPVATSVLAAAALTACGGGDDVPSNDLPAGITQHGV
                     TVYSATTTGAGTTAATQDLLTAGLGKSGIGAAAAPAYADPLNPTALELRRNAVYSNYR
                     GLIDYSAGGGYGTLYGPNVDVGGTATAGEGLVPGREYVATLDDGSGSKRVVIAVQIPD
                     SFDTHAPCIVLGPSSGSRGVYGAVATAGDWGLKHGCAVALTDAGKGVGLYDLSDDTVN
                     KIDGTRATRTAAGVLSFFAANLSDAVRAAYNAAFPNRVALKQVHSQLNPEKDWGNDTL
                     AAARYAFYALNDRYGSTFDPVPFRADNTIVIAGSASNGGAAVLRAGELDTGALIDGIV
                     AAEPVAELPTTSGYGIQFGGAAVGGYGKRLADFTTYGNVYQPCAALAAGAAMTEVSVF
                     NYLVLTAQTGKAAARCDALAAKGLVSGADTAARALDALNKLRAYGWTSDNDQMHNAHY
                     ALGNGPILSAMYPVMYGRFSVADNVCDTSFAQVDGFGAPVPVAAAAKAQSFAIANGTA
                     NGTPATVVYNNSVGGARSWVLATSPSSGTQDFGLDNALCQRALVTGVDETGAALTATA
                     TSTRPTAAQSAAVQAGINEVVVNGNLHAKPTLVVAGRSDALVPVNNNARAYAAFNRSV
                     EGGASKLGYIEVTNAQHFDTFIPFSGFDTRYVPLHLYYVRAMDAMYAHLKNGTPLPPS
                     QVVRTTPRGGLPGAAPAITGAAVPPLAAAPLAANQIGFSGNALAVPN"
     gene            4551..5390
                     /locus_tag="VFA35_00055"
     CDS             4551..5390
                     /locus_tag="VFA35_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT54587.1"
                     /translation="MSFTSNYLTCAGRELHYTEWGAQHRETVIAWHGLARTGRDMDDL
                     AAHLATRYRVICPDTIGRGLSQWSPAPEREYCLAFYEQLAVALADALGLDRFHWVGTS
                     MGGAIGTRAAANGLRGRVRRLVLNDNGPELAQAAIARIRSYAGSPAAFACVSELEQYF
                     RTVYKPYGWLSDAQWRRLTETSTRRLPDGRVTPHYDPSMVMQFTHHADDYRQWDDWDR
                     LDIPVLCLRGEHSDLLLRETTDQMRQRGPRAIVVEIAGCGHAPALNTPDQWALVERFL
                     AAA"
     gene            complement(5414..7477)
                     /locus_tag="VFA35_00060"
     CDS             complement(5414..7477)
                     /locus_tag="VFA35_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT54588.1"
                     /translation="MSDDLPESRSRAKPPASQGLLGAAARNDRRLFDSLNEGVWERDL
                     VTQEVWYSPRYKSLLGFADDELPNSIEFVRSRLHPADLAGVRQAFAHAARTLSTGEAQ
                     ARVRVKDGSYRWFRGRFTVWPDASGKPAILVGALYDVHEQVVATEALKAQHAVLEQRV
                     HERTQGLATALQQAEAARHAAEQANQAKASFLAHMSHELRTPLNGVLGMNQLAQALAS
                     GAEQRRYLELAQQAGQALLGFLDDVLDFARAETGRLQLRDEPVDLAALASETLRSFMP
                     SVRRRGLQVGFDYVGDVTQLRGDAGRLRQMIANLLGNAIKFTEHGQVLLIVAVKPGAD
                     GAPCRVRLRVRDSGVGMDAATVARVFEPFEQGDLGLARRHGGTGLGLSVVRMLARLMG
                     GEVAVRSRPGQGSEFRVDLRLAAAPAPPRCARAAGHAWVLARDAGHGGGLCKRLVRLG
                     GTAEVLADLGAAVGRLQAHDASAPDAVVIAEDMLAAATDFESLRRGLRAGVPVTLLLR
                     PDFDLGAVHAAAERWQLRIAIAPLTPADLHALLHADAPAPHAPPPRRQPQDRPPSVLV
                     VEDNPMNQIIAREMVAALGLQPAVVGSGEEAVRSCRSMPPDLVLMDIQMPGMDGLETT
                     RRLRALQDAGELRRFPIIALTAHALGADRQASLAAGMDEHLTKPVQLEQLRGVLNRWL
                     VADRG"
     gene            7614..>8169
                     /locus_tag="VFA35_00065"
     CDS             7614..>8169
                     /locus_tag="VFA35_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT54589.1"
                     /translation="MASGLIRIRGARQHNLKNLDLDIRTGEMTVVTGPSGSGKSSLVF
                     DTLYAEGQRRYVETFSAYARQFLDRMDRPAVDRVDGVPPAIAIDQTNPVRSSRSTVGT
                     MTELNDHLKLLYARAAQLFDRQTAQSVRHDSPETIYAELMARTAGSDPRLVVTFPVEL
                     PGGTSAEEVEQWLAASGFTRVQAER"
BASE COUNT         1128 a         3023 c         2806 g         1212 t
ORIGIN      
        1 gcaaccacgg tccttctggt cgagcagaac ttcgccatgg ccaaggcgct cggcgacacg
       61 gtcgcggtga tggacgacgg ccgcgtcgtc caccgcggcg agatggcggc gctggccgag
      121 gacgagggcc tgcagcacaa gctgctcggc ttgtcgctgg cgacccacca gtgagggccc
      181 aggcatgagc gagctccccg ccgtccccgg atccggcgtc gccccggccg ctgccgaagc
      241 gctgccgcag acccgcttcg acttcctgcc gctgatcctc gtcgcggtga tcgccctggc
      301 ggcctggccc gcggtcggct cgagctcgac ctggctgacg ctcaccttcg ccggcctggc
      361 gatgggcatg atcgtcttca tcgccgcttc cggcctgaca ctcgtcttcg gcctgatgga
      421 cgtgctcaac ttcggccaca gcctgttcat cgccctcggc gcctatatcg ctaccagcgt
      481 attcgccgcg accgtcggct ggacctcggt cgccagcctg gcgcccacgc tcgctgcggt
      541 cggcctggcg atgctcgcgg cgatggtcct cggcggcgtc ttcggcctcg gcttcgagcg
      601 ggtcatcgtc cgcccggtct acggccagca cctgaagcag atcctggtga cgatcggcgg
      661 cgcgatcgtc ggcgaggagc tgatcaaggt cgtctggggg ccggcgcaga tcccgcttcc
      721 gccgcccgag gcgctgcgcg gctcgatcgt catcggcgag gcgaccgtcg acaagctgcg
      781 cctggtgtcg gtggcgatcg gtctggtcgt cctcgcggcg ctcctctgga ccctcaaccg
      841 caccaagatc ggcctcctga tccgcgccgg cgtccaggac cgcgagatgg tcgaaagcct
      901 cggctaccgg atccggcgcc tgttcgtcgg cgtcttcgcc gccggctcgg cgctggccgg
      961 gctcggcggc agcctctggg gcatgaacca gcagatcgtg gtgccgcaga tcggcggcca
     1021 ggtcaacgtg ctgctcttca tcgtcatcat catcggcggc ctcggctcca cgctcggctg
     1081 cctggtcggt gcgctcgtcg tcggcctgct gaccaactac gtcggcttcc tcgcaccgaa
     1141 gctgaccatg ttctcgagca tcggcctgat ggtggcggtg ctgatctggc ggccgcaggg
     1201 cctctatccc gtgacgagcc gctgacccat gctcgatcgc ctcgtctccc acgaccggcc
     1261 gcgcaaccgc tggctggcgc tggtgctgct cctgatcgtc gtcggcctgg cgctgacgcc
     1321 gttcatcttc cccgggacca aggcgctcaa cgtcgccgcc aaggtcatcg tcttcgtcgt
     1381 cctcgcggcg agcttcgacc tgctcctcgg ctacaccggc atcgtcagct tcgcgcacac
     1441 catgtttttc ggcatcggcg cctacggcat cgccatcgcc accacccggc tgggcccgac
     1501 ctggggcgcg ctcgccagcg gggcgctggt cgcgctggtg ctctcggcgc tgctgtcgtt
     1561 cgtgatcggc ctgttcagcc tgcgcgtcaa ggcgatcttc ttcgcgatga tcacgctcgc
     1621 ggtcgcctcc gcgttcctcg cgctcgcctc gcagctctcg gcgttcaccg gcggcgagga
     1681 cggcctgagc ttcaaggtgc ccgtcgcgct ccagcccggc accgagtacc tcgccgagcc
     1741 cctgctcggc gcctcgatcg acggccgctt cgtcacctac tacgggctgt tcttcgccgc
     1801 gctcggcctc gtgctgatgc tgctgcgcat cgtcaactcg ccgttcggcc gcgtgttgca
     1861 ggcgatccgc gagaacgact tccgcgccga ggcgatcggc taccgcacgg tcgtgtaccg
     1921 cacgctcgcg tcggtgctgt cggcgctgtt cgcgacactc gccggcgtgc tgctcgcgct
     1981 ctggctgcgc tacaacgggc cggacacgac gctctcgttc gagatcatgc tcgacgtgct
     2041 gctgatcgtc gtgatcggcg gcatgggcac ggtctacggc gcggtcatcg gcagcgtgct
     2101 gctggtgctc gcgcagaact atctgcaaga cctgatgaag ctcggcgcgt ccgccgtcga
     2161 gggcatcccg gtcgtctcgc aactcgtctc gcccgaccgc tggctgctct ggctgggcat
     2221 cctgttcgtg ctgtcggtct atcactttcc gagcggtgtg gtcgggcgtt tgcgcgcgag
     2281 cgcgagacga ccctgaccgc gtgtcgagca accccgagca acccgaggag acaagtgcca
     2341 tgaagacgat ccagcgttcc taccttccgg tggcgacgag cgtgctcgcc gcggcggccc
     2401 tgacggcctg cggcggcggt gacgacgtgc cgtcgaacga cctgccggcc ggcatcacgc
     2461 agcacggcgt cacggtctat tcggccacca ccaccggcgc cggcaccacc gcggcgacgc
     2521 aagatctgct gaccgccggc ctgggcaaga gcggcatcgg cgccgccgcc gcgccggcct
     2581 acgccgaccc gctgaacccg accgcgctcg agctgcgccg caacgccgtc tacagcaact
     2641 accgcgggct catcgactac agcgccggcg gtggctacgg cacgctctac ggcccgaacg
     2701 tcgacgtggg cggcacggcc accgcgggcg agggcctggt gcccggccgc gagtacgtcg
     2761 ccacgctcga cgacggcagc ggcagcaagc gcgtcgtgat cgcggtgcag atccccgaca
     2821 gcttcgacac ccacgcgccg tgcatcgtgc tcgggccgtc gtcgggttcg cgcggcgtct
     2881 atggcgcggt cgcgaccgcc ggcgattggg gcctgaagca cggctgtgcg gtcgcgctga
     2941 ccgacgccgg caagggcgtc ggcctgtacg acctgagcga cgacaccgtc aacaagatcg
     3001 acggcacgcg cgccacgcgc acggccgccg gcgtgctcag tttcttcgcc gccaacctca
     3061 gcgacgcggt gcgcgcggcc tacaacgccg cgttcccgaa tcgggtcgcg ctcaagcagg
     3121 tgcactcgca gctgaacccc gagaaggact ggggcaacga cacgctggcc gcggcgcgct
     3181 acgcgttcta cgcgctcaac gaccgctacg gcagcacgtt cgatccggtg ccgttccgcg
     3241 ccgacaacac gatcgtgatc gccggctcgg cctcgaacgg cggcgccgcg gtgctgcgcg
     3301 ccggcgagct cgacaccggc gcactgatcg acggcatcgt cgccgccgag cccgttgccg
     3361 agctgccgac gaccagcggc tacggcatcc agttcggcgg cgccgcggtc ggcggctacg
     3421 gcaagcggct cgccgacttc acgacctacg gcaacgtcta ccagccctgc gccgcgctcg
     3481 ccgccggcgc cgcgatgacc gaggtctcgg tcttcaacta cctcgtgctg accgcgcaga
     3541 ccggcaaggc cgcggcgcgc tgcgacgcgc tcgccgcgaa gggcctggtg agcggcgccg
     3601 acacggcggc gcgcgcgctc gacgccttga acaagctgcg cgcctacggc tggacgagcg
     3661 acaacgacca gatgcacaac gcccactacg cgctcggcaa cggcccgatc ctgtcggcga
     3721 tgtacccggt gatgtacggc aggttctcgg tcgccgacaa cgtctgcgac accagcttcg
     3781 cgcaggtcga cgggttcggc gcgccggtcc cggtggcggc cgccgccaag gcgcagagct
     3841 tcgcgatcgc caacggcacc gccaacggca cgcccgcgac cgtggtctac aacaactcgg
     3901 tcggcggcgc gcgcagctgg gtgctcgcga cctcgccgtc gtcgggcacg caggacttcg
     3961 gcctcgacaa cgcgctgtgc cagcgcgcgc tcgtcaccgg cgtcgacgag accggcgcgg
     4021 cgctgaccgc cactgccacc tcgacccggc cgaccgcggc gcagagcgcg gcggtgcagg
     4081 ccggcatcaa cgaggtcgtc gtgaatggca acctgcacgc gaagccgacg ctggtggtcg
     4141 ccggccgcag cgatgcgctg gtgccggtga acaacaacgc gcgcgcctac gcggccttca
     4201 accgcagcgt cgaaggcggc gcgtcgaagc tcggctacat cgaggtgacg aacgcgcagc
     4261 acttcgacac cttcatcccg ttcagcggct tcgacacgcg ctacgtgccg ctgcacctgt
     4321 actacgtgcg ggcgatggac gcgatgtacg cgcacctgaa gaacggcacg ccgctgccgc
     4381 cgagccaggt ggtgcgcacc acgccgcgcg gcggcttgcc cggcgccgca ccggccatca
     4441 ccggggccgc cgtaccgccg ctcgccgcgg cgccgctcgc cgcgaaccag atcggcttca
     4501 gcggcaacgc gctcgcggtt ccgaactgag atcgttcacc agcccttgtc atgagtttca
     4561 cgtcgaacta cctcacttgc gcgggccgcg aactccacta caccgaatgg ggcgcgcagc
     4621 accgcgagac cgtgatcgca tggcatgggc tggcacgcac cgggcgcgac atggacgatc
     4681 tggccgccca cctcgccacc cgctaccgcg tgatctgccc cgacacgatc ggccgcgggc
     4741 tgagccagtg gagccccgcg ccggaacgcg agtactgcct cgcgttctac gagcaactgg
     4801 cggtcgcgct ggccgacgcg ctgggcctcg atcgcttcca ctgggtcggt acctcgatgg
     4861 gcggcgcgat cggcacgcgc gccgcggcca acggcctgcg cggccgggtg cgccgcctgg
     4921 tcctgaacga caacggcccc gagctcgcgc aggccgcgat cgcgcgcatc cgcagctacg
     4981 ccggcagccc ggccgcgttc gcgtgcgtga gcgagctgga gcagtacttc cgcacggtct
     5041 acaagcccta cgggtggctc agtgacgcgc agtggcggcg cctgaccgaa acctcgacgc
     5101 ggcgcctgcc cgacggccgc gtgacgccgc actacgaccc gtcgatggtg atgcagttca
     5161 cccaccacgc cgacgactac cggcaatggg acgactggga ccggctcgac atcccggtgc
     5221 tgtgcctgcg cggcgagcac tcggacctgc tgctgcgcga gaccaccgac caaatgcgcc
     5281 agcgcgggcc gcgcgcgatc gtcgtcgaga tcgccggttg cgggcacgcg ccggcgttga
     5341 acacgccgga tcagtgggcg ctggtggagc ggttcttggc cgctgcctga cggcccggcg
     5401 gggcccgaat cagtcagcct cgatcggcga ccagccaccg gttcaacacg ccgcgcaact
     5461 gctccagttg caccggcttc gtcaggtgct cgtccatgcc ggcggccagg ctggcctggc
     5521 ggtcggcgcc gagcgcgtgc gcggtcagcg cgatgatcgg aaagcggcgc agttcgcccg
     5581 cgtcctgcaa cgcgcgcagg cggcgcgtgg tttcgaggcc atccatgccg ggcatctgga
     5641 tgtccatcag caccaggtcg ggcggcatgc tgcggcagct gcgcaccgcc tcctcgccgc
     5701 tgccgacgac cgcgggctgc agcccgagcg cggcgaccat ctcgcgcgcg atgatctggt
     5761 tcatcgggtt gtcttcgacg acgagcacgc tcggtgggcg gtcttgcggc tgccgccgcg
     5821 gcggcggcgc gtgcggcgcg ggcgcatcgg cgtgcagcag cgcatgcagg tcggccggcg
     5881 tcagcggcgc gatcgcgatc cgcagctgcc agcgttcggc ggccgcatgc acggcgccga
     5941 ggtcgaagtc ggggcgcagc agcagcgtca ccggcacgcc ggcgcgcagg ccgcgacgca
     6001 gcgattcgaa atcggtggcg gcggccagca tgtcttcggc aatcaccacg gcgtcgggcg
     6061 cgctcgcgtc gtgcgcttgc aggcggccga ccgccgcgcc caggtcggcc agcacctcgg
     6121 ccgtgccgcc caggcgcacg agccgcttgc acaggccgcc cccgtggccg gcgtcgcgcg
     6181 ccagcaccca ggcgtggccg gccgcgcgcg cacaccgcgg tggcgcgggc gcggccgcga
     6241 gccgcaagtc gacgcggaac tcggagccct ggccgggccg gctgcgcacc gccacctcgc
     6301 cgcccatcag ccgcgccagc atgcgcacca ccgacagccc gagcccggtg ccgccgtggc
     6361 gccgcgccag ccccaagtcg ccctgctcga agggctcgaa cacgcgcgcg acggtggccg
     6421 cgtccatgcc gacgccgctg tcgcgcacgc gcagccgcac gcgacagggc gcgccgtcgg
     6481 cgccgggctt gaccgcgacg atcagcagca cctggccgtg ttcggtgaac ttgatcgcgt
     6541 tgccgagcag gttggcgatc atctgtcgca gccggccggc gtcgccgcgc agctgcgtga
     6601 catcaccgac atagtcgaag ccgacctgca ggccccggcg gcgcaccgag ggcatgaagc
     6661 tgcgcagcgt ctcgctggcg agcgcggcga ggtcgaccgg ctcgtcgcgc aattgcaggc
     6721 ggccggtttc ggcgcgcgcg aaatcgagca cgtcgtcgag aaaaccgagc agcgcctgcc
     6781 cggcctgctg cgcgagttcg aggtagcggc gctgctcggc gccgctcgcc agcgcctgcg
     6841 cgagctggtt catgccgagc acgccgttca gcggcgtgcg cagctcgtgg ctcatgtggg
     6901 ccaggaaact tgccttggcc tggttcgcct gctcggccgc gtgccgcgcg gcctcggcct
     6961 gctgcagcgc cgtcgcgagg ccttgggtgc gctcgtgaac gcgctgttcg agcaccgcat
     7021 gctgggcctt cagggcttcg gtggcgacga cctgctcgtg cacgtcgtag agcgcgccga
     7081 ccaggatcgc cggcttgccg cttgcgtcgg gccagacggt gaagcggccg cgaaaccagc
     7141 ggtagctgcc gtccttcaca cgcacgcgtg cctgcgcctc gccggtgctc agcgtgcgcg
     7201 ccgcatgcgc gaaggcctgg cgcacgccgg ccaggtcggc cgggtggagc cggctgcgca
     7261 cgaattcgat cgagttcggc aactcgtcgt cggcgaagcc gagcagcgac ttgtagcgcg
     7321 gcgagtacca gacctcctgg gtgacgagat cgcgctccca gacgccttcg ttcaggctgt
     7381 cgaacagccg gcgatcgttg cgcgcggcgg cgcccagcag gccttgcgat gcgggcggtt
     7441 tcgcgcggga tctcgactcg ggcagatcgt cactcatggg gcgggctggc gatgcgggcg
     7501 aaccgcggat attagccgcg gcggcgcgtg caggtacggt ctttgcacgc ctgccggctc
     7561 caaagtcacg tcctccctag aatgttcggc tcgctcagga aagcagttcc ctcatggcct
     7621 ccggtctgat ccgcatccgc ggcgcgcgcc agcacaacct caagaacctc gacctcgaca
     7681 tccgcaccgg cgaaatgacg gtcgtgaccg ggccgagcgg ctcgggcaag tcgagcctgg
     7741 tgttcgacac cttgtatgcc gaggggcagc gccgctatgt cgagaccttc agcgcctacg
     7801 cgcggcagtt cctcgaccgc atggaccgcc cggcggtcga ccgggtcgac ggcgtgcccc
     7861 ccgcgatcgc gatcgaccag acgaatccgg tgcgcagctc gcgctcgacc gtcggcacga
     7921 tgaccgagct gaacgaccac ctgaagctgc tctacgcgcg cgcggcgcag ctgttcgaca
     7981 ggcagacggc gcaatcggtg cgccacgatt cgcccgagac catctacgcc gagctgatgg
     8041 cgcgcaccgc cggcagcgac ccgcggctgg tcgtcacctt cccggtcgag ctgcccggcg
     8101 gcaccagcgc ggaggaagtc gagcaatggc tcgcggcgag cggcttcacg cgcgtgcagg
     8161 ccgagcgcg
//
LOCUS       DASRDG010000005         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1035888, whole genome shotgun sequence.
ACCESSION   DASRDG010000005 DASRDG010000000
VERSION     DASRDG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035888"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VFA35_00070"
     CDS             <1..1131
                     /locus_tag="VFA35_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZT54590.1"
                     /translation="GTAMAMWAMTVLVAPVAGPLLGGWITDNISWPWIFYINIPVGIV
                     AAFITWSIYHKRDPGPTKVPLDWVGLVLLVAFVGTMQVMIDKGKELDWFNSGQIVTLA
                     VVSAVSFMFFLAWELTDKHPIVDLRLFARRNFLMGSVALSVAYGLFFGNVVLLPLWLQ
                     QYMGYTATWAGLATAPVGILAIILSPWVGKNVAKIDPRKLATVAFLGFGGVLWMRSNF
                     NTQADFATILIPTVLQGAAMAFFFIPLQAIVFSGLTPDKLPSAAGLSNFVRITMGAVG
                     TSLFTTLWENRAILHHARLTEAISTTNDTATQVGAQLAAAGLGTEQAAASVNRMIDQQ
                     AYTMAVTDVFYMSSLLFFALIALIWFAKPQPGGAGGGAGGAH"
     gene            complement(1147..2223)
                     /locus_tag="VFA35_00075"
     CDS             complement(1147..2223)
                     /locus_tag="VFA35_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HZT54591.1"
                     /translation="MDRRSLLLCLAATPAFARAQAGLHAGLHFPADFGAHPETRTEWW
                     YATGSLVSGARLWGFQVTFFRSSTGVKAAAGSRFAPTQLLFAHAALTDLEAARLRHDQ
                     RIARSGFGIAAARGADTGLVLRDWQMDRTPSPADAGRSRYRARVASERAGFAFELQLD
                     ATQPVLLQGDAGVSKKGPGANQTSRYYSEPQLAVQGTLTLDGRPVEVTGRAWLDHEWS
                     DGFMPPQAVGWDWIGMNLDDGSALTAFALRRADGSAFYAGGSLRRAGEAVRPFGPDQV
                     TFTPGRVWESASSRARYPVEWSVATPAGRFGVRALLDDQELDSRGSSGAIYWEGLSDL
                     LDDAGRRVGRGYLEMTGYAAALRL"
BASE COUNT          329 a          846 c          828 g          364 t
ORIGIN      
        1 ggcaccgcga tggcgatgtg ggcgatgacg gtgctcgtcg cgccggtggc cgggccgctg
       61 ctcggcggct ggatcaccga caacatctcg tggccgtgga tcttctatat caacatcccg
      121 gtcggcatcg tcgccgcgtt catcacgtgg tcgatctatc acaagcgcga cccggggccg
      181 acgaaggtgc cgctcgactg ggtcggcctc gtcctgctgg tcgcgttcgt cggcacgatg
      241 caggtcatga tcgacaaggg caaggagctc gactggttca attcaggcca gatcgtgacg
      301 ctcgcggtcg tgtccgcggt gagcttcatg ttcttcctcg cgtgggagct gaccgacaag
      361 catccgatcg tcgacctgcg cctgttcgcg cggcgcaact tcctgatggg ctcggtggcg
      421 ctgtcggtcg cctacgggct gttcttcggc aacgtcgtgc tgctgccgct gtggctgcag
      481 cagtacatgg gctacaccgc gacctgggcg ggcctggcga ccgcgccggt cggcatcctc
      541 gcgatcatcc tgtcgccgtg ggtcggcaag aacgtcgcca agatcgaccc gcgcaagctc
      601 gcgaccgtcg ccttcctcgg cttcggcggg gtgctgtgga tgcgctcgaa cttcaacacc
      661 caggccgact tcgccacgat cctgatcccc accgtgctgc aaggcgcggc gatggcgttc
      721 ttcttcatcc cgctgcaggc gatcgtgttc tcggggctca cgcccgacaa gctgccctcc
      781 gcggcgggct tgtcgaactt cgtgcggatc acgatgggcg cggtcggcac ctcgctgttc
      841 acgacgctgt gggagaaccg cgcgatcctg caccacgcgc ggctcaccga agcgatcagc
      901 acgaccaacg acaccgcgac gcaggtcggc gcgcagctgg cggccgccgg cctcggcacc
      961 gagcaggcgg ccgcgagcgt caaccgcatg atcgaccagc aggcctacac gatggcggtg
     1021 accgacgtgt tctacatgtc gtcgctgctg ttcttcgcgc tgatcgcgct gatctggttc
     1081 gcgaagccgc aacccggcgg cgcgggcggc ggcgccggcg gtgcgcactg atcgaaccac
     1141 cgcgcgctac aggcgcagcg cggcggcgta gcccgtcatc tcgaggtagc cgcggccgac
     1201 gcggcggccc gcgtcgtcga gcaggtcgct gagcccctcc cagtagatcg cgccgctgct
     1261 gccgcggctg tcgagctcct ggtcgtcgag cagggcgcgc acgccgaagc ggccggccgg
     1321 cgtcgcgacc gaccactcga ccgggtagcg cgcgcgcgaa ctcgcgcttt cccagacgcg
     1381 gccgggcgtg aacgtgacct ggtcgggtcc gaaggggcgc accgcttcgc cggcgcggcg
     1441 caggctgccg ccggcgtaga acgcgctgcc gtcggcgcgg cgcagggcaa acgcggtgag
     1501 cgcgctgccg tcgtcgaggt tcatgccgat ccagtcccag ccgaccgcct gcggcggcat
     1561 gaagccgtcg ctccactcgt ggtcgagcca ggcgcggccg gtgacttcga ccggccggcc
     1621 gtcgagcgtc agcgtgcctt gcaccgcgag ctgcggctcg ctgtagtagc ggctcgtctg
     1681 gttcgcgccc ggccccttct tcgacacgcc ggcatcgcct tgcagcagca ccggctgcgt
     1741 cgcatccaac tgcagctcga acgcgaagcc ggcgcgctcg ctcgcgaccc gcgcgcggta
     1801 gcggctgcgc ccggcatcgg cgggcgaggg cgtgcggtcc atttgccagt cgcgcagcac
     1861 caggccggtg tcggcgccgc gcgctgccgc aatgccgaag ccgctgcgcg cgatgcgctg
     1921 gtcgtggcgc aggcgagcgg cctcgaggtc ggtcagcgcg gcgtgcgcga acagcagctg
     1981 ggtcggcgcg aaccggctgc ccgctgccgc tttgacgccg gtcgacgatc ggaagaacgt
     2041 gacctggaag ccccacagcc gcgcgccgct gaccaggctg ccggtcgcgt accaccactc
     2101 ggtgcgcgtc tcggggtgcg cgccgaagtc ggccgggaaa tgcaaacccg cgtgcagccc
     2161 tgcctgcgcg cgcgcgaatg cgggcgtggc ggccaagcac agcagcaggg agcgtcggtc
     2221 cacacgcgca agcatcgcac agccgtgggc ggacaatgcc ccgcatggcc aagcccgttc
     2281 cccgactcag ccccgccgaa gtccagcgtg tgatcgcgac cgcctgggac gaccacccgc
     2341 cgttcaacgc cgtgttgatg gcccatg
//
LOCUS       DASRDG010000006         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1151679, whole genome shotgun sequence.
ACCESSION   DASRDG010000006 DASRDG010000000
VERSION     DASRDG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151679"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFA35_00080"
     CDS             <1..523
                     /locus_tag="VFA35_00080"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HZT54592.1"
                     /translation="GKVAADIAWGGNWFAFVEASQLGLHVDKAHLPSLMPAAAAVRQA
                     LLDSGVRGVHPQRGGEEVVDHVKLFDELHGERAPGARALTLCPGAAYDRSPCGTGTSA
                     KLAVLHAKGELAAGQWFTSESVLGTRFRGRVASTTSVGPHAAVVPEIEGAAWITGLQQ
                     FVIDPDDPCRFGI"
     gene            543..1364
                     /locus_tag="VFA35_00085"
     CDS             543..1364
                     /locus_tag="VFA35_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HZT54593.1"
                     /translation="MPATLTRTAAALLAAAAACAPLQPQPASAESPIDHAIATAAGPT
                     EAARARSPGARARPLPSWKVVGHSVQGRPLRAATYGSGARRVLWIGGIHGNEREGAVA
                     TDELPDAFLAAPGGERAVTLTVLEDVNPDGSAMHMRGNANGVDLNRNFPADNFVANRM
                     FGREPLSQPESLALRELITAVQPHLVLIAHSWSGDHFVNFDGPARELADRFAQLSGYP
                     VRRSEDIPATPGSLGSWLGQELGVPVLTIEYLRGREPGEAWRETRDAVLAVVLDG"
     gene            1367..>2072
                     /locus_tag="VFA35_00090"
     CDS             1367..>2072
                     /locus_tag="VFA35_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT54594.1"
                     /translation="MLRWLCVRCAWMVPSLIGITCLTFLLLDLAPLDRARLEVLAEPT
                     ALDTRQDREMRVLRLEIRYGLVDERTLERVPVVTRYMRWLGNALRFRLAGPGEDQQQF
                     RARIAAALPVTLLLGFWSLAFAFGAGVPLGIWLGMRADGRADRMASGFLFVAAGLPEV
                     LIATLLLLAFGFLLPWFPSGGLHSDGIAARSWFVQLLDLAWHLALPVAAMSLPPLLLV
                     ARFLRESVRRAAATSFA"
BASE COUNT          229 a          778 c          766 g          299 t
ORIGIN      
        1 cggcaaggtc gcggcagaca tcgcgtgggg cggcaactgg ttcgcgttcg tcgaggcgag
       61 ccagctcggc ctgcatgtcg acaaggcgca cctgccttcg ctgatgcccg cggcggccgc
      121 cgtgcggcag gcgctgctcg actccggcgt ccgcggcgtg cacccgcagc gcggcggcga
      181 ggaggtcgtc gaccacgtca agctgttcga cgagctgcac ggcgaacgcg cgcccggcgc
      241 gcgcgcgctg acgctgtgcc ccggcgccgc ctacgaccgc tcgccgtgcg gcaccggcac
      301 gtcggcgaag ctcgccgtgc tgcacgccaa gggcgagctc gcggcggggc aatggttcac
      361 gtcggagagc gtgctcggca cgcgcttccg cggccgcgtg gcgtcgacca ccagcgtcgg
      421 cccgcacgcc gcggtggtgc ccgagatcga gggcgcggcg tggatcaccg ggctgcagca
      481 gttcgtcatc gaccccgacg atccgtgccg cttcgggatc tgaccgctat cgtgcgcgcg
      541 tcgtgcctgc gaccctcacc cgcaccgccg ccgcgctgct cgctgccgcc gcggcgtgcg
      601 cgccgctgca gccgcagccg gcgtccgcgg agtcgccgat cgaccacgcg atcgcgacgg
      661 cggccggtcc caccgaggcc gcgcgcgcgc gcagcccggg cgcccgggcg cggccgctgc
      721 cgtcgtggaa ggtcgtcggg cacagcgtgc agggccggcc gctgcgcgcc gcgacgtacg
      781 gctctggcgc gcgccgcgtg ctgtggatcg gcggcatcca cggcaacgag cgcgagggcg
      841 cggtcgcgac cgacgagctg ccggacgcgt tcctcgcggc acccggcggc gaacgggccg
      901 tgacgctcac ggtgctcgag gacgtcaacc ccgacggctc ggcgatgcac atgcgcggca
      961 acgccaacgg cgtcgatctc aaccgcaact tcccggccga caacttcgtc gcgaaccgca
     1021 tgttcggccg cgagccgctg agccagcccg agtcgctcgc gctgcgcgag ctgatcaccg
     1081 cggtgcagcc gcacctggtg ctgatcgcgc acagctggag cggggatcac ttcgtgaact
     1141 tcgacgggcc cgcgcgcgag ctcgccgacc gcttcgcgca gctgtcgggc tatcccgtgc
     1201 ggcgctccga ggacatcccg gcgacgccgg gatcgctcgg cagctggctc ggccaggagc
     1261 tcggcgtgcc ggtgctcacg atcgaatacc tgcgcggccg cgagccgggc gaggcgtggc
     1321 gcgagaccag ggatgcggtg ctcgccgtgg tgctcgacgg ctgaccatgc tgcgctggct
     1381 ctgcgtgcgc tgcgcgtgga tggtgccgag cctgatcggc atcacctgcc tcacgttcct
     1441 gctgctcgac ctcgcgccgc tcgaccgcgc gcgcctcgaa gtgctcgccg agccgacggc
     1501 gctcgacacc cgccaggacc gcgagatgag ggtgctcagg ctcgagatcc gctacgggct
     1561 cgtcgacgag cgcacgctcg agcgcgtgcc ggtggtcacg cgctacatgc gctggctcgg
     1621 caacgcgctg cggttccggc tcgcgggtcc gggcgaggac cagcagcagt tccgcgcgcg
     1681 gatcgccgcg gcgctgccgg tgacgctgct gctcggcttc tggtcgctcg cgttcgcgtt
     1741 cggcgccggc gtcccgctcg gcatctggct cggcatgcgc gccgacggcc gcgccgaccg
     1801 catggcttcg ggtttcctgt tcgtcgccgc cggcctgccc gaggtgctca tcgccaccct
     1861 gctgctgctg gcgttcggct tcctgctgcc gtggttcccg agcgggggcc tgcacagcga
     1921 cggcatcgcc gcgcgctcgt ggttcgtgca gctgctcgat ctcgcgtggc acctcgcgct
     1981 gccggtggcg gcgatgtcgc tgccgccgct gctgctggtc gcgcgcttcc tgcgcgaatc
     2041 ggtgcgccgc gccgcggcga cgtcgttcgc gg
//
LOCUS       DASRDG010000007         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_116294, whole genome shotgun sequence.
ACCESSION   DASRDG010000007 DASRDG010000000
VERSION     DASRDG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116294"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..764
                     /locus_tag="VFA35_00095"
     CDS             105..764
                     /locus_tag="VFA35_00095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZT54595.1"
                     /translation="MSYRYLKLDDTIYRYVLDHTLREIPEQAALRAATLPLPDAGMQI
                     SPEQGQFMALLVRLIGARRTLEIGVFTGYSALSVALALPDDGELIACDISDEYTRIGR
                     PFWAAAGVAHKIDLRLAPALDTLDALLAQGRSGSFDFAFIDADKAGYDAYYERCLQLL
                     RPGGLIAIDNVLWSGAVAKPATSADTLALQQLNDKLLRDERIDLSMLPIGDGLSLARK
                     R"
     gene            complement(790..1482)
                     /locus_tag="VFA35_00100"
     CDS             complement(790..1482)
                     /locus_tag="VFA35_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HZT54596.1"
                     /translation="MIEVYSWATPNGHKVHIMLEECGLPYRVHPIDIGAGDQFSPEFL
                     AISPNNKIPAIVDPNGPDGAPISLFESGAILMYLAGKTGRFMPASTRGRYAVLEWLMF
                     QMGSVGPMLGQTHHFRLYAPEKIEYAVNRYTNEARRLYGVMNTRLAKSKYMAGSEYGI
                     ADIAIFPWLRSWKNQGIAWVDYPHLRGWFDEIAARPAVQRGVEVLASQRRPLVDDKAR
                     EMLFGDTQYKKH"
     gene            1553..2293
                     /locus_tag="VFA35_00105"
     CDS             1553..2293
                     /locus_tag="VFA35_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54597.1"
                     /translation="MLDAFKRLFSRPPPARDLGLIEEWAKRRGHGFKRARGEDGFVID
                     GLLEGKPWRIEWGPSQREYIAGHELRLRMELDLPSDAQMLVLSRPLMDALERKTFEEF
                     TDNVQTQIGTKTPEEMRWLVMFPKVNLAHLKTLRNRFGAVSSAPDTGLSWINGPLANM
                     LEQAANEWLHDGQPLVLMTLRGRAYLRTQLDAPDPTLLAAALALFETAVAQAVRASGG
                     PVDRAGDAGHSGRSTAWQSFPPEVPPQR"
BASE COUNT          368 a          792 c          774 g          377 t
ORIGIN      
        1 atgcgcgcga agcgatgcgg gtggccgcgt actggaagcg cggcgcgcgg gattatcacg
       61 acgagcttgg ttgaaccctc gccaccatcg acacggagaa acccatgagc taccgctacc
      121 tgaagctcga cgacacgatc tatcgttatg tgctcgacca tacgctgcgc gaaatcccgg
      181 aacaggcggc gctgcgtgcc gcgacgctac ccttgccgga tgccggcatg cagatctcgc
      241 ccgagcaggg ccagttcatg gcgctgctgg tgcgcctgat cggcgcgcgg cgcacgctcg
      301 agatcggcgt gttcaccggc tacagcgcgc tcagcgtggc gcttgcgctg cccgacgacg
      361 gggagctgat cgcctgcgac atcagcgacg agtacacccg catcggccgt ccgttctggg
      421 ccgcggccgg cgtcgcgcac aagatcgacc tgcggctcgc cccggcgctc gacacgctcg
      481 acgcattgct cgcgcaaggc cgctccggca gcttcgactt cgcgttcatc gacgccgaca
      541 aggcgggcta cgacgcctac tacgaacgct gcctgcagct gctgcggccc ggcggcctga
      601 tcgcaatcga caacgtgttg tggagcgggg cggttgcaaa gccggcaacg agcgccgaca
      661 cgctggcctt gcagcaactc aacgacaagc tgctgcgcga cgaacgcatc gacctctcga
      721 tgctgccgat cggagacggg ctgagcctgg cgcgcaaacg ctgacgcggg tccggctcga
      781 ccgcgacgct cagtgcttct tgtactgcgt gtccccgaac agcatctcgc gcgccttgtc
      841 gtccacgagc ggcctgcgct gcgacgccag cacctcgaca ccgcgctgca ccgccggccg
      901 ggcggcgatc tcgtcgaacc agcccctcag gtgcgggtag tcgacccagg cgatgccctg
      961 gttcttccat gagcgcaacc acgggaagat cgcgatgtcg gcgattccgt actcgctgcc
     1021 ggccatgtat ttgctcttcg ccaggcgcgt gttcatcaca ccgtaaagcc gcctggcctc
     1081 gttggtgtag cggttcaccg cgtactcgat cttctcgggc gcgtagaggc ggaagtgatg
     1141 tgtctgcccg agcatcggcc cgacgctgcc catctggaac atcagccact cgagcaccgc
     1201 atagcgcccg cgcgtgctcg ccggcatgaa gcggccggtc ttgccggcca ggtacatgag
     1261 gatcgcgccc gactcgaaca gcgagatcgg tgcgccgtcc ggcccgttcg gatcgacgat
     1321 cgcggggatc ttgttgttcg ggctgatcgc caggaactcg gggctgaact ggtcgcccgc
     1381 gccgatgtcg atcggatgca cgcgataggg caggccgcac tcttcgagca tgatgtgaac
     1441 cttgtgtccg ttcggcgtgg cccacgaata cacttcgatc atgtctgcac tcatgctgtc
     1501 ctcgggtggt ctgctgcgtt ctagcggcca ctctagttga tcgggcgcac cgatgctcga
     1561 tgcgttcaaa cgcctgttct cgcgcccgcc accggcgcgc gacctcggcc tcatcgagga
     1621 atgggcgaag cgccgcggcc acggcttcaa gcgcgcgcgc ggcgaggacg gcttcgtgat
     1681 cgacggcctg ctcgaaggca agccctggcg catcgagtgg gggccgtcgc agcgcgagta
     1741 catcgccggc cacgagttgc gactgcggat ggagctcgat ctgccgtcgg acgcgcagat
     1801 gctggtgctg tcgcgcccgc tgatggatgc gctggagcgc aagaccttcg aggagttcac
     1861 cgacaacgtc cagacccaga tcggcaccaa gacgcccgag gagatgcgct ggctcgtgat
     1921 gttcccgaag gtcaacctcg cgcacctgaa gacgctgcgc aaccgcttcg gcgcggtctc
     1981 gagcgcgccg gacaccggcc tgtcgtggat caacgggccg ctggcgaata tgctcgagca
     2041 agcggcgaac gagtggctgc acgacgggca gccgctggtg ctgatgacct tgcgcggccg
     2101 tgcctacctg cgcacgcaac tcgacgcgcc cgacccgacg ttgttggccg cggcgctcgc
     2161 gctgttcgag acggcggtgg cgcaggccgt gcgcgcttcc ggcgggccgg tcgatcgcgc
     2221 cggcgacgcc ggccacagcg gccgcagcac ggcgtggcag agcttcccgc ccgaggtgcc
     2281 gccgcagcgt tgacgagcgc ggcgcgcgcg c
//
LOCUS       DASRDG010000008         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1184, whole genome shotgun sequence.
ACCESSION   DASRDG010000008 DASRDG010000000
VERSION     DASRDG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..477)
                     /locus_tag="VFA35_00110"
     CDS             complement(34..477)
                     /locus_tag="VFA35_00110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZT54598.1"
                     /translation="MNQVQPGSFLTLHYRLSGPDGADLINTFDDKPATLSLGTGELAP
                     AMEARLLGLPEGTRQSFELAAGEAFGERNPELLQRVKRSLLNELGDPDERYSVGDVVQ
                     FPTPDGQGAYAGVVREVDGDVLVFDFNHPLAGRPVTFEVQLIGVL"
     gene            561..1235
                     /gene="radC"
                     /locus_tag="VFA35_00115"
     CDS             561..1235
                     /gene="radC"
                     /locus_tag="VFA35_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZT54599.1"
                     /translation="MVLKDIPADARPREKLLASGAAALADAELIALLLRTGFKGTTVL
                     QLAQQMLDHFGGLAGLLHAQADDLKRIKGLGPAKRAELAAVIELARRALAQELAQRPA
                     FDHPAKVKDYLRLQLGKHPHEVFAVLFLDAQARLLKFEEMFRGTLTQTSVYPREVVKR
                     ALELHAASVVLAHNHPSGVAQPSKADEYLTQTLVSALKLVDVRVLDHFVVGEGEVISF
                     AERGLL"
     gene            1232..1909
                     /locus_tag="VFA35_00120"
     CDS             1232..1909
                     /locus_tag="VFA35_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZT54600.1"
                     /translation="MKARTLAELGPIGKALRDAKRAAAEAERADRERRAREQRERDLF
                     ALTVGAVTPLRAVAVAVAPRRPRPPATARQRERDEAAVMLEAISDEFDVESLLDTDDA
                     LSFRRPGIGPEVVRKLRRGVWAIQAQLDLHGLRRDEARERLNRFIFEARRRGLRCVRV
                     IHGKGNGSPGREPVLKAKVKSWLVQTKDVIAFTQARASDGGHGALLVLLGPAAPKPGA
                     QMAPHAR"
     gene            complement(1906..2310)
                     /locus_tag="VFA35_00125"
     CDS             complement(1906..2310)
                     /locus_tag="VFA35_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HZT54601.1"
                     /translation="MQIDPPKTPTPFERIGGQPGTRALVDRFYDLMDLDPAFAELRAL
                     HPTTLDGSRDKLFWFLCGWLGGPPLYTDRFGHPMLRARHLPFAIGIKERDQWLACMQQ
                     AMGETQVEPALAQRLAESFFGTADWMRNKPGA"
BASE COUNT          380 a          881 c          864 g          343 t
ORIGIN      
        1 cggcaagcac gatctcttgc aactccgaat tcatcacagc actccgatca actgcacttc
       61 gaaggtcacc gggcggccgg cgagcgggtg gttgaagtcg aacaccagca cgtcgccatc
      121 gacctcgcgc accacgccgg catacgcgcc ctgcccgtcg ggcgtcggga actgcaccac
      181 gtcgccgacg ctgtagcgct cgtcgggatc gcccagctcg ttcagcagcg aacgcttcac
      241 gcgctgcagc aactcggggt tgcgctcgcc gaaggcctcg ccggcggcga gctcgaacga
      301 ctgccgcgta ccttcgggca aacccagcaa acgcgcttcc atcgccggcg cgagttcacc
      361 ggtgccgagc gacaaggtcg cgggcttgtc gtcgaaagtg ttgatgaggt cggcgccgtc
      421 ggggcccgac aggcggtagt gcagggtcaa gaaggacccc ggttggactt ggttcaagcg
      481 caatcctgac aaagcatgaa gccgatagac tgaggccgat tttacgagcc ggccgcaatg
      541 cccttggatt gatctgactc atggtgctca aggacatccc cgccgacgcc cgcccgcgcg
      601 agaagctgct cgccagcggc gccgccgcgc tcgccgacgc cgagctgatc gcgctgctgc
      661 tgcgcaccgg cttcaagggc accacggtgc tgcagctcgc gcagcagatg ctcgaccatt
      721 tcggcggcct cgccggcctg ctgcacgcgc aggccgacga cctgaagcgg atcaagggcc
      781 tgggcccggc caagcgcgcc gaactcgccg cggtgatcga actcgcgcgc cgcgcgctcg
      841 cgcaggagct ggcgcaacgc ccggcgttcg accacccggc caaggtgaag gactacctgc
      901 ggctgcaact cggcaagcac ccgcacgagg tcttcgccgt gctgtttctc gacgcgcagg
      961 cgcggctgct gaagttcgag gagatgttcc gcggcacgct cacgcagacc agcgtctacc
     1021 cgcgcgaggt cgtcaagcgc gcgctcgagc tgcacgccgc ctcggtcgtg ctcgcgcaca
     1081 accacccgtc gggcgtggcg caaccgtcga aggccgacga gtacctcacg cagacgctgg
     1141 tcagcgcgct gaagctggtc gacgtgcgtg tgctcgatca cttcgtcgtc ggcgagggcg
     1201 aggtgatctc gttcgccgaa cgtggactcc tgtgaaggcc cgcacgctgg ccgaactcgg
     1261 cccgatcggc aaggcgctgc gcgacgcgaa gcgggccgcc gccgaggccg agcgcgcgga
     1321 ccgggaacgc cgcgcacgcg agcagcgcga gcgcgacctg ttcgcgctga cggtcggcgc
     1381 ggtcacgcca ctgcgcgccg tcgccgtcgc cgtcgcgccg cgccgcccca ggccgccggc
     1441 gaccgcgcgc caacgcgaac gcgacgaggc cgcggtgatg ctggaggcga tctcggacga
     1501 gttcgatgtc gagtcgctgc tcgacaccga cgacgcgctg agctttcgcc gcccgggcat
     1561 cggccccgag gtcgtgcgca agctgcgccg cggcgtctgg gcgatccagg cgcagctcga
     1621 cctgcacgga ctgcgccgcg acgaggcgcg cgagcgcctg aaccgcttca tcttcgaggc
     1681 gcgccgccgc ggcctgcgct gcgtgcgcgt gatccacggc aagggcaacg gctcgccggg
     1741 ccgcgagccg gtgctcaagg ccaaggtcaa gagctggctc gtgcagacga aggacgtgat
     1801 cgcgttcacg caggcgcgcg ccagcgacgg cggccacggg gcgctgctcg tgctgctcgg
     1861 gcctgcggcg ccgaagcccg gcgcgcagat ggcaccacac gcgcgttagg cgccgggctt
     1921 gttgcgcatc cagtcggccg tgccgaagaa cgattcggcc agccgctgcg cgagtgccgg
     1981 ttcgacctgc gtctcgccca tcgcctgctg catgcacgcg agccactgat cgcgctcctt
     2041 gatgccgatg gcaaacggca ggtgccgcgc gcgcagcatc gggtgcccga agcgatcggt
     2101 gtagagcggc gggccgccga gccagccgca gaggaaccag aacagcttgt cgcgcgagcc
     2161 gtcgagcgtg gtcgggtgca gtgcgcgcag ttccgcgaac gccgggtcca ggtccatcag
     2221 gtcgtagaag cggtcgacga gcgcgcgcgt gccgggctgg ccgccgatgc gctcgaacgg
     2281 ggtcggtgtc ttcggggggt cgatttgcat cagggcgcga ttttcgccgg gatcgtcccc
     2341 ggaaccgtcc ctgcgatcgt cgccgcgatc gcctcgaccg cctgcgccgc ggcgctgccg
     2401 ggcagcatct cgaccagcag ctggcggctc tgcaccgcct cgcgcaccgc cgggtcgagc
     2461 ggcacctc
//
LOCUS       DASRDG010000009         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_693360, whole genome shotgun sequence.
ACCESSION   DASRDG010000009 DASRDG010000000
VERSION     DASRDG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693360"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2152)
                     /locus_tag="VFA35_00130"
     CDS             complement(<1..2152)
                     /locus_tag="VFA35_00130"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZT54602.1"
                     /translation="MCSQSEPMLMHVGMPSLYPSNVQEILDFGLHGYLMSRLSGLWVG
                     LKIVTNIADGHGQADVTPERLNFVTPDLTFDGQPFTPRMNLGMNVRAEAIEMEQSLYT
                     RRLEVAKRYAAANKLDNVTFGNADAWLGIITAGKSYNDLRQAFFELGLDDAALKRYGI
                     RILKMGMLFPMEPQVVRDFARGLEEIFVVEEKRPFLEMFAKNVLYGMANAPRIVGKFD
                     EEEKELLPHYGEFEADIIGRALVRRLSRKTRVESAEAWLARVDEIHARTKLPTAGRTA
                     WFCSGCPHNTSTQPVDGSVVSAGIGCHTMAMWMDRNVVMGTHMGAEGSQWIGMAPFTD
                     QKHIFQNMGDGTYAHSGSLAIRYCASTNTNITFKVLRNLHTSMTGGQQIQGEEALAQM
                     VSEMLANGVKKIIVTSDRPERISGLPGGTEVWHRDRVDEAQRALEAVPGTTVLIHNQE
                     CAAELRRARSRGKADEPVEVTVINERVCEGCGDCGVKSNCMSVEPVQTEFGRKTRIHQ
                     SSCNKDFSCVKGFCPSFLTITPNPEPAAADGKKKKKKGRIPALERALPDPANKIDDRV
                     GFNIHVMGIGGTGSVTVVATLANAARLEGKHVIGLDQTGLAQKGGAVISDIKVTHEPF
                     TGSSKVSDGRTDLYLGFDILNATDPKNLDKCLPERTIAVVSTTKTPTGQMVSNRKVLF
                     PIVEDLTAGIAKVTRTADNVFLDGQGLAEGLFGDA"
BASE COUNT          299 a          721 c          741 g          410 t
ORIGIN      
        1 tcgcgtcgcc gaacaggcct tcggccaggc cttggccgtc gaggaacacg ttgtcggccg
       61 tgcgggtcac cttggcgatg ccggcggtga ggtcctcgac gatcgggaac agcaccttgc
      121 ggttgctcac catctggccg gtcggcgtct tggtggtcga gaccaccgcg atcgtgcgct
      181 cgggcaggca cttgtcgagg ttcttcgggt cggtcgcgtt caggatgtcg aagcccaggt
      241 acaggtcggt gcggccgtcg gagaccttgc tcgacccggt gaagggctcg tgcgtgacct
      301 tgatgtccga gatcaccgcg ccgccctttt gcgccaggcc ggtctggtcc aggccgatga
      361 catgcttgcc ttcgagccgc gcggcattgg cgagcgtcgc caccacggtc accgagccgg
      421 tgccgccgat gcccatcacg tggatgttga agccgacccg gtcgtcgatc ttgttcgccg
      481 gatcgggcag cgcgcgctcg agcgccggga tgcggccctt ctttttcttc ttcttgccgt
      541 cggccgccgc cggttcgggg ttcggcgtga tcgtcaggaa cgacgggcag aagcccttga
      601 cgcacgagaa gtccttgttg cacgaggact ggtggatgcg cgtcttgcgg ccgaactcgg
      661 tctgcaccgg ctcgacgctc atgcagttgc tcttgacgcc gcagtcgccg cagccttcgc
      721 agacgcgctc gttgatgacc gtgacctcga ccggctcgtc ggccttgccg cggctgcggg
      781 cgcggcgcag ttcggccgcg cactcctggt tgtggatcag caccgtcgtg ccgggcaccg
      841 cctcgagcgc gcgctgcgcc tcgtcgacgc ggtcgcgatg ccagacctcg gtgccgccgg
      901 gcaggccgct gatgcgctcg ggccggtcgc tggtgacgat gatcttcttg acgccgttgg
      961 cgagcatctc cgagaccatc tgcgcgagcg cctcctcgcc ctggatctgc tgcccgccgg
     1021 tcatcgacgt gtgcaggttg cgcagcacct tgaaggtgat gttggtgttc gtgctggcgc
     1081 agtagcggat cgcgaggctc cccgagtggg cgtaggtgcc gtcgcccatg ttctggaaga
     1141 tgtgcttctg gtcggtgaac ggcgccatgc cgatccactg cgagccctcg gcgcccatgt
     1201 gcgtgcccat cacgacattg cggtccatcc acatcgccat cgtgtggcag ccgatgccgg
     1261 ccgagacgac gctgccgtcg accggctgcg tgctcgtgtt gtgcgggcaa cccgagcaga
     1321 accaggcggt gcggcccgcg gtgggcagct tggtgcgggc gtggatctcg tcgacgcggg
     1381 ccagccaggc ctcggccgat tcgacccgcg tcttgcgcga cagccggcgc accagcgcgc
     1441 ggccgatgat gtcggcctcg aactcgccgt agtgcggcag cagttccttc tcctcctcgt
     1501 cgaacttgcc gacgatgcgc ggcgcgttcg ccatgccgta cagcacgttc ttcgcgaaca
     1561 tctcgaggaa cgggcgcttc tcctcgacga cgaagatctc ctcgagaccg cgcgcgaagt
     1621 cgcgcaccac ctgcggctcc atcgggaaca gcatgcccat cttcaggatg cggatgccgt
     1681 agcgcttgag cgcggcgtcg tcgaggccga gctcgaagaa ggcctggcgc aggtcgttgt
     1741 agctcttgcc ggcggtgatg atgcccagcc aggcgtcggc gttgccgaac gtgacgttgt
     1801 cgagcttgtt ggccgccgcg tagcgcttgg ccacctcgag ccggcgcgtg tacagcgact
     1861 gctccatctc gatcgcctcg gcgcgcacgt tcatgccgag gttcatgcgc ggcgtgaacg
     1921 gctggccgtc gaacgtcagg tcgggcgtga cgaagttcag ccgctcaggc gtcacgtcgg
     1981 cctgtccatg accgtcggcg atgttggtga cgatcttcag ccccacccac agtcccgaca
     2041 gccgcgacat caggtagccg tgcagcccga agtccaggat ctcctgcacg ttggacgggt
     2101 acagcgaggg catgccgacg tgcatcagca tcggctcgga ctggctgcac aacgaggagc
     2161 tcttgcagct c
//
LOCUS       DASRDG010000010         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_807228, whole genome shotgun sequence.
ACCESSION   DASRDG010000010 DASRDG010000000
VERSION     DASRDG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807228"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /gene="hutU"
                     /locus_tag="VFA35_00135"
     CDS             complement(<1..1728)
                     /gene="hutU"
                     /locus_tag="VFA35_00135"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZT54603.1"
                     /translation="MNSTTDLSLGKNALADAAVRGARCAPGRVVRAPQGSTLHCKSWL
                     TEAAFRMLQNNLDPDVAERPEELVVYGGIGRAARDWACFDKILDCLRVLEDDESLLIQ
                     SGKPVGVFKTHADAPRVLLANSNLVPKWATWEHFHELDRQGLFMYGQMTAGSWIYIGT
                     QGIVQGTYETFAEAARQHHGGSLAGKWILTAGLGGMGGAQPLAASFAGAVSLTIECRQ
                     ESIDFRLRTRYVDEQAADLADALARIAKYTAEGKAVSIALLGNAATFVPALAEHFANG
                     AANRPALVTDQTSAHDLINGYLPAGWSVREWLAAQADSSRHAELKAAAAKSCAAHVQA
                     LLRFQAMGVPTVDYGNNIRQVAFDEGVKNAFDFPGFVPAYIRPLFCEGKGPFRWVALS
                     GDPEDIRKTDAKIKQLFPHNTHVHRWLDMAAERIAFQGLPARICWLGLGERHLAGLAF
                     NAMVKSGELKAPIVIGRDHLDTGSVASPNRETEAMKDGTDAVSDWPLLNALLNVAGGA
                     TWVSLHHGGGVGMGYSQHAGVVIVCDGTPEAAKRIARVLRNDPGTGVMRHADAGYDSA
                     IRSANENGLD"
     gene            complement(1817..2773)
                     /locus_tag="VFA35_00140"
     CDS             complement(1817..2773)
                     /locus_tag="VFA35_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZT54604.1"
                     /translation="MTTALNFRTLDLNLLRVFDVVMAERNITRAAERLSITQPAVSNA
                     LKRLKNSVGEDLLTRVSTGVKPTPRAEALWPEVRTALGHLRAALAPGEFNPQTDATSF
                     RIAMADATAALLMPPLVSQIERDEVLTNLSVLPLTTRDPGELLARGDADLAIGYFPET
                     IRALTGQGGDSPLRHAHLHDSEYVCVMREGHRLACDGALTLDSFCAADHLLVNFAGRP
                     YGLADQALAALNRKRRIVLTVNQYFTAGRVVANSNLLTVLPACFIEATGCHEQVVTRP
                     LPFDLAGLHVAMVWHQRNDHHSAHQWLRARLLEAAARRTGDG"
BASE COUNT          430 a          956 c         1054 g          488 t
ORIGIN      
        1 atccagtcca ttttcattcg cggatcggat tgcgctatca tatccggcgt cggcgtggcg
       61 catcacgccg gtgccggggt cgttgcgaag cacgcgagcg atccgcttcg cagcttccgg
      121 tgtgccatcg cacacgatca ccacgcccgc gtgctgcgag tagcccatgc cgacaccgcc
      181 gccgtggtgc agcgagaccc aggtcgcgcc gccggcgacg ttcaagagcg cgttcaggag
      241 cggccaatcg gagacggcgt cggtgccgtc cttcatggcc tcggtctcgc ggttcgggct
      301 tgcgaccgag ccggtatcga ggtggtcgcg gccgatcacg atcggcgcct tcagctcgcc
      361 cgatttgacc atcgcgttga acgcgaggcc ggcgaggtgg cgctcgccca ggccgagcca
      421 gcagatgcgc gcgggcaggc cctggaacgc gatgcgctcg gcggccatgt cgagccagcg
      481 gtgcacatgg gtgttgtgcg ggaacagttg cttgatcttc gcgtcggtct tgcggatgtc
      541 ttccgggtcg cccgacagcg cgacccagcg gaacgggccc ttgccttcgc agaacagcgg
      601 ccggatgtag gccggcacga agccggggaa gtcgaacgcg ttcttcacgc cttcgtcgaa
      661 cgcgacctgg cggatgttgt tgccgtagtc gacggtgggc acgcccatcg cctggaagcg
      721 cagcagggcc tggacgtgcg cggcgcagct cttcgccgcg gcagccttca gctcggcgtg
      781 gcgcgacgaa tcggcctgcg cggccagcca ttcccggacc gaccagccgg ccggcaggta
      841 gccgttgatc aggtcgtgcg ccgaggtctg gtcggtcacc agcgcggggc ggttggcggc
      901 gccgttcgcg aagtgctcgg ccagcgccgg cacgaaggtc gcggcgttgc cgagcagcgc
      961 gatcgacacg gccttgccct cggccgtgta cttcgcgatg cgggcgagcg cgtcggccag
     1021 gtcggcggcc tgctcgtcga cgtagcgggt gcgcagccgg aagtcgatgc tctcctggcg
     1081 gcactcgatg gtcagcgaga cggcgccggc gaagctcgcg gccagcggct gcgcgccgcc
     1141 catgccgccc aggccggcgg tgaggatcca cttgccggcg aggctgccgc cgtggtgctg
     1201 gcgtgcggcc tcggcgaagg tttcatacgt gccctgcacg atgccttgcg tgccgatgta
     1261 gatccacgac ccggcggtca tctgcccgta catgaacagg ccctggcggt cgagttcgtg
     1321 gaagtgctcc caggtcgccc acttcggcac gaggttggag ttcgcgagca gcacgcgcgg
     1381 cgcgtcggcg tgggttttga acacacccac cggcttgccc gactggatca gcagcgattc
     1441 gtcgtcctcg agcacgcgca ggcagtcgag gatcttgtcg aagcaagccc agtcgcgcgc
     1501 ggcgcggccg atgccgccgt agacgacgag ttcctcgggg cgctcggcca cgtcggggtc
     1561 gaggttgttt tgcagcatgc ggaacgcggc ctcggtgagc cacgacttgc agtgcagcgt
     1621 gctgccctgc ggcgcgcgca cgactcggcc gggcgcgcag cgcgcgccgc gcacggcggc
     1681 atcggccaag gcgttcttgc cgagtgagag gtcggtcgtg gagttcatcg cgcgtcgctt
     1741 cccgtgctgt tggtatgcag atgaggcaaa gtggtctaga ccactttgcg cagcatgcgg
     1801 gaagacgcgt gccgcgtcaa ccgtcgccgg tgcgccgggc tgcggcttca agcagccgcg
     1861 cgcgcagcca ctggtgcgcg ctgtggtggt cgttgcgctg gtgccagacc atcgcgacgt
     1921 gcaagccggc caggtcgaac ggcagcggtc gggtcacgac ctgctcgtgg cagccggttg
     1981 cctcgatgaa gcaggccggg agcacggtca gcaggttgct gttcgcgacg acgcggccgg
     2041 cggtgaagta ctggttcacc gtcagcacga tgcgacgctt gcggttcagc gccgccagcg
     2101 cctgatcggc caggccgtac gggcggccgg cgaagttgac gagcaggtga tcggccgcgc
     2161 agaagctgtc gagcgtcaac gcgccgtcgc aggcaagccg gtggccctcg cgcatcacgc
     2221 agacgtactc gctgtcgtgc aggtgcgcgt ggcgcagcgg cgagtcgccg ccctggccag
     2281 tgagtgcccg gatcgtttcg gggaagtagc cgatcgccag gtcggcgtcg ccgcgcgcca
     2341 gcagctcgcc cgggtcgcgg gtggtcagcg gcagcacgct caggttggtc agcacctcgt
     2401 cgcgctcgat ttgcgacacc agcggcggca tcagcagcgc cgcggtggcg tcggccatcg
     2461 cgatccggaa actggtcgca tcggtctgcg ggttgaactc gcccggcgcc agcgcggcgc
     2521 gcagatgccc cagtgcggtg cgcacttcgg gccacagggc ttcggcccgg ggcgtcggct
     2581 tgacgcccgt cgagactctt gtgagcaagt cctcacccac cgaattcttc aggcgcttca
     2641 acgcattgct gaccgccggc tgggtgatcg acaagcgctc ggcggcgcgc gtgatgttgc
     2701 gctcggccat caccacgtcg aagacgcgca gcaggttcag gtcgagggtg cggaagttca
     2761 gggcggtggt catcgtcata cgtcgtcgtg ataccggcga ttcataagat tgattggtgg
     2821 attatgcggg tttaccccaa tatctccaca acgaggccat cacggccccg gcgtttgaag
     2881 gagatttcgc atgaccactg ccaccctgca cgccaatgtg caacgcaa
//
LOCUS       DASRDG010000011         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_232066, whole genome shotgun sequence.
ACCESSION   DASRDG010000011 DASRDG010000000
VERSION     DASRDG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232066"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFA35_00145"
     CDS             <1..390
                     /locus_tag="VFA35_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HZT54605.1"
                     /translation="RSVCAEMHAGFGALRGHLGMNIEASLPEVGARVLAEQPQVRDDI
                     ARIVAMWGELLEQHGGPMLFGAFGIADAFFAPVVTRLRTYGVSVPAPIAAYIERVLAL
                     PGVAAWIAGALAEHDFLAFEEAYRTQR"
     gene            complement(434..937)
                     /locus_tag="VFA35_00150"
     CDS             complement(434..937)
                     /locus_tag="VFA35_00150"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HZT54606.1"
                     /translation="MKFDKIIAAAALSLAAASSFADSGGGALDLSTGSTFFGRTPSGS
                     SFVDTYTFTLTGGSFLTTGSVDSSAIGGQDIDFTGITIMRGATTVASFANGGTDAQEH
                     YTLAQTLLTPGNYALVVTGTQSVDAASYAGNLAMVAAPVPEPEAYAMLIAGLGVLGFV
                     ARRRRQR"
     gene            1071..>2173
                     /locus_tag="VFA35_00155"
     CDS             1071..>2173
                     /locus_tag="VFA35_00155"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HZT54607.1"
                     /translation="MESYMVGGAVRDQLQHLPVTDRDWVVVGATPEQMLALGFKPVGV
                     DFPVFLHPETREEYALARTERKVAPGYRGFRFHAAPDVTLEDDLRRRDLTINAIAQAA
                     DGTLIDPCGGRRDLAAGLLRHVSPAFAEDPVRILRVARFAARFTTFTVAPETLALMRE
                     MVAAGEVDALVPERVWQELARGLMEAQPSRMFEVLRACGALARLLPEVDRLWGVPQRA
                     DYHPEVDTGVHLMLVLDMAARLDASLAVRFACLGHDLGKGTTPVDVLPRHLGHEARSV
                     ALVREVCERLRVPVACRELAEVVAREHGNVHRSAEFGAAAVLRLIERCDAIRRPDRFA
                     DMLLACECDARGRLGLEESAYPQRERLGRALARA"
BASE COUNT          284 a          725 c          812 g          352 t
ORIGIN      
        1 cgcagcgtgt gcgccgagat gcacgcgggc ttcggcgcgc tgcgcggtca cctcgggatg
       61 aacatcgagg cctcgctgcc cgaggtcggc gcgcgcgtgc tggccgagca gccgcaggtg
      121 cgcgacgaca tcgcgcgcat cgtcgcgatg tggggcgagc tgctcgaaca gcacggcggg
      181 ccgatgctgt tcggcgcctt cggcatcgcc gatgcgttct ttgcgccggt ggtgacgcgg
      241 ctgcgcacct acggcgtttc ggtgccggcg ccgatcgcgg cgtacatcga gcgcgtgctc
      301 gcgctgccgg gggttgctgc ctggatcgcc ggggcgctgg ccgagcacga cttcctggcg
      361 ttcgaagagg cgtatcgaac gcagcgttga accgcgcttc gcgagcgcgg ttcaacaagg
      421 tgattgcgcg cgcctaccgc tggcgacggc ggcgtgcgac gaagccgaga acgcccaggc
      481 ctgcgatcag catcgcatag gcttcgggtt cgggcaccgg tgccgcgacc atggccagat
      541 tgcccgcata cgaggctgcg tccacgcttt gcgtgccggt gaccaccagc gcgtagttgc
      601 ccggcgtcag cagcgtctgc gcgagcgtgt agtgctcttg cgcatcggtg ccgccgttcg
      661 cgaagctcgc gacggtggtg gcgccgcgca tgatcgtgat tccggtgaag tcgatgtcct
      721 gaccgccgat ggccgaactg tcgaccgagc cggtcgtcag gaacgacccg ccggtgagcg
      781 tgaaggtgta ggtgtcgacg aagctcgagc ccgacggcgt gcgaccgaag aaggtgctgc
      841 cggtgctcag gtcgagcgcg ccgccgccgc tgtcggcgaa gctgctggcg gcggccagcg
      901 agagcgctgc cgcggcgatg attttgtcga atttcatgcg tgtccttttt agcgagtggg
      961 cgcacgtgaa gggtccgtgc cggttgggtt ataggtgccg caccggcctg gccacacccg
     1021 tgatcgcggg ggtgaaaccc aagtcgcgcc atgtgcctac actcggggta atggagtcct
     1081 acatggtggg cggcgcggtc cgcgatcagc tgcagcatct gcctgtgaca gaccgtgact
     1141 gggtcgtcgt cggtgccacg cccgagcaga tgctcgcgct cggcttcaag ccggtcgggg
     1201 tcgactttcc ggtcttcctg caccccgaaa cgcgcgagga atacgcgctc gcccgcaccg
     1261 agcgcaaggt cgcacccggc taccgcggct ttcgcttcca tgcggcaccc gatgtgacgc
     1321 tcgaagacga tctgcggcgc cgcgacctga ccatcaacgc gatcgcgcag gccgccgacg
     1381 gcacgctgat cgacccgtgc ggcgggcgcc gcgatctcgc ggccgggctg ctgcgccacg
     1441 tctcgccggc gttcgccgaa gacccggtgc gcatcctgcg ggtggcgcgc ttcgcggcgc
     1501 ggttcacgac cttcacggtc gcgcccgaga ccctcgcgct gatgcgtgag atggtcgcgg
     1561 ccggcgaggt cgatgcgctc gtgcccgagc gcgtctggca ggagctggcg cgcggcctga
     1621 tggaagcgca gccttcgcgc atgttcgagg tgctgcgcgc atgcggcgcg cttgcgcggc
     1681 tgctgcccga ggtcgatcgc ctgtggggcg taccgcagcg cgccgactac caccccgagg
     1741 tcgacaccgg cgtgcacctg atgttggtgc tcgacatggc ggcgcgcctg gacgccagcc
     1801 tcgcagtgcg cttcgcgtgc ctgggccacg accttggcaa gggcacgacg ccggtcgacg
     1861 tgctgccgcg ccacctgggg cacgaagcgc gcagcgtcgc gttggtgcgc gaggtgtgcg
     1921 agcgcctgcg cgtgccggtg gcgtgccgcg agctcgccga ggtcgtcgcg cgcgagcatg
     1981 gcaacgtgca ccgcagcgcg gagttcggcg ccgcggccgt gctgcgattg atcgagcgct
     2041 gcgacgcgat ccgccgcccg gaccggtttg ccgacatgct gctagcgtgc gagtgcgatg
     2101 cgcgcgggcg gctcgggctc gaggagtcgg cgtatccgca gcgcgagcgg ctcggccgtg
     2161 cgctcgcgcg ggc
//
LOCUS       DASRDG010000012         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1268039, whole genome shotgun sequence.
ACCESSION   DASRDG010000012 DASRDG010000000
VERSION     DASRDG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268039"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFA35_00160"
     CDS             complement(<1..233)
                     /locus_tag="VFA35_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54608.1"
                     /translation="MTQTKLLKAAGLGHEISAREAWKLFGIMAEFFEATERLAGVRPA
                     VSVLGSARVKPETLYYRLAEETARLLSQAGFAV"
     gene            262..>2063
                     /locus_tag="VFA35_00165"
     CDS             262..>2063
                     /locus_tag="VFA35_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HZT54609.1"
                     /translation="MLLVDGSSYLYRAFHALPELRSPKTGEPTGAIYGVLNMLRKLAT
                     DYKAQARVAVFDAKGRTFRDELYPQYKATRTAMPDDLSRQVEPLHDAITALGWPLLVI
                     DGVEADDVIATLVEQARARGWHTVISTGDKDLTQLVDARTLWINTMSNEKLDVAGVTA
                     KFGVPPEKIVDYLALIGDQIDNIPGVDKVGPKTAAKWLNAYGTLASIQEHAGEITGKV
                     GENLRAGTETVALSRQLATIRRDVDLPVDEAALRPRAPDVAALRELYTRLEMRSFLRA
                     LDGDATAGGAVGEVAGGGAGGPSGGAGAVRTTVAVAAAGTAAAAGAAGATPALGVDPV
                     ERHYVMIDDWTVFLGWLERLRRAPLIAFDTETTGLDYMKAQIVGVSFCVEAGRGAYVP
                     LAHGYPGAPDQLDRNRVLAALRPLLEDPDLPKVGANLKFDMHVLENHGIVLRGQRYDT
                     MLESYVLNSVATRHDMDSMAGRYLGIATIHFEDVAGKGAKQITFDQVDVATATTYAAE
                     DADVTLRLHQALWPQLAAVPALEKLYTGIEQPLVPVLQRMERGGVLIDRAMLRQQSIE
                     LAARLKELGAQAQREAGQEFNVESPRQLQQILFE"
BASE COUNT          342 a          724 c          704 g          293 t
ORIGIN      
        1 aaaacggcga acccggcctg cgagagcagc cgcgcggtct cttccgcgag ccggtagtag
       61 agcgtctcgg gtttcacgcg cgcgctgccc aacacgctga ccgcgggccg cacgccggcg
      121 aggcgctcgg tcgcctcgaa gaactctgcc ataatgccga acagcttcca cgcctcgcgc
      181 gcggagatct cgtggccgag gccggccgcc ttcagtagct tcgtctgtgt catcgtgcct
      241 ggagagagtc cggaaaaaac gttgttgctg gtcgacggct cgtcgtacct gtatcgcgcc
      301 tttcacgcgc tgcccgagct gaggagcccg aagaccggcg agccgaccgg cgccatctat
      361 ggcgtgctga acatgctgcg gaagctcgcc accgactaca aggcgcaggc gcgcgtcgcg
      421 gtgttcgacg cgaagggccg cacgttccgc gacgagctct atccgcaata caaggcgacg
      481 cgcacggcga tgcccgatga cctctcgcgc caggtcgagc cgctgcacga tgcgatcacc
      541 gcgctcggct ggccgctgct cgtcatcgac ggcgtcgaag ccgacgacgt gatcgccacg
      601 ctggtcgagc aggcgagggc gcgcggctgg cacaccgtca tctccaccgg cgacaaggac
      661 ctgacgcagc tcgtcgacgc gcgcacgctc tggatcaaca ccatgtcgaa cgagaagctc
      721 gacgtcgccg gggtcaccgc caagttcggc gtaccgccgg agaagatcgt cgactacctg
      781 gcgctgatcg gcgaccagat cgacaacatc ccgggcgtcg acaaggtcgg tccgaagacg
      841 gcggccaagt ggctcaacgc ctacggtacg ctggcgtcga tccaggaaca tgccggcgag
      901 atcacgggca aggtgggcga gaacctgcgc gccggcaccg agacggtcgc gctctcgcgc
      961 cagctggcca cgatccgccg tgacgtggac ctgcccgtcg acgaggcggc gctgcggccg
     1021 cgcgcgcccg acgtcgcggc gttgcgcgag ctgtacacgc gcctcgagat gcgctcgttc
     1081 ctgcgtgcgc tcgatgggga cgccaccgca ggcggcgctg tcggcgaagt tgcaggcggc
     1141 ggcgctggcg gaccgtcggg cggcgcgggc gcggtccgca ccacggtcgc agtggcagcc
     1201 gccggcacgg cggcggcagc cggggccgct ggcgcaacgc ccgcgctggg cgtcgatccg
     1261 gtcgaacggc attacgtgat gatcgacgac tggaccgtgt tcctcggctg gctcgagcgc
     1321 ttgcggcgtg ctccgctcat cgccttcgac actgaaacca cggggctcga ctacatgaag
     1381 gcgcagatcg tgggcgtctc cttctgcgtc gaggccgggc gcggcgccta cgtgccgctc
     1441 gcgcacggct atcccggcgc gcccgaccag ctcgaccgca atcgcgtgct cgcggcgctg
     1501 cgcccgctgc tcgaggatcc cgacctcccc aaggtcggcg ccaacctgaa gttcgacatg
     1561 cacgtgctcg agaaccacgg catcgtgctg cgcggccagc gctatgacac gatgctcgag
     1621 tcctacgtgc tcaacagcgt cgcgacgcgc catgacatgg actcgatggc cggccgctac
     1681 ctcggcatcg cgacgatcca cttcgaggat gtcgccggca agggcgcgaa gcagatcacc
     1741 ttcgaccagg tggacgtggc caccgcgacc acctatgccg ccgaggacgc cgacgtcacg
     1801 ctgcgcctgc accaggcact ctggccgcag ctcgccgccg tgccggcgct ggaaaaactc
     1861 tacacgggga tcgaacagcc gctggtgccg gtgctgcagc gcatggagcg cggcggcgtg
     1921 ctcatcgatc gggcgatgct gcgccagcag agcatcgaac tggccgcgcg tctcaaggag
     1981 ctcggcgcgc aggcgcagcg cgaggccggc caggaattca acgtcgaatc gccccggcag
     2041 ctgcagcaga tcctgttcga gaa
//
LOCUS       DASRDG010000013         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1154904, whole genome shotgun sequence.
ACCESSION   DASRDG010000013 DASRDG010000000
VERSION     DASRDG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154904"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VFA35_00170"
     CDS             <1..1726
                     /locus_tag="VFA35_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZT54610.1"
                     /translation="QVAELPGYEEAGPDTAEAILDEAAKFASQVLAPMNREGDTVGCK
                     WRDGEVTTPPGNKQAYAQYIAGGWTGLAFPQQYGGQGLPKLLATAVHDIEQSANLGFS
                     LAPGLSRGAIEALLLCGTDAQKERYLHRMIAGDWTGTMNLTEPQAGSDLGLLRTKAEP
                     DGDHYRLYGQKIFISHGEHDLAENIVHLVLARLPDAPEGVRGISLFVAPKFLVNDDGT
                     LGARNDIRCVSIEHKMGLHASPTCVLAYGDREGAIGTLIGEPNRGLEYMFIMMNEARF
                     GVGLSGIAIAERAYQQALAYAKERVQSKDVANPRGPSVTIINHPDVRRMLMTMKALIE
                     STRALCYVVAGAMDHAHASPDEAERARARAFTEIVVPIHKAWSTEMALEVGNLAIQVH
                     GGMGFIEETGVSQHMRDARILSIYEGTTGIQANDLIGRKIARDGGATAKALAEQMKGA
                     LAELSGNDDDSQAIRARLADSIATFERCVDWIVANTKADVKGVHAGAVPFLKLAALTC
                     GGWQMARAAAVAQRKLAAGEGDAEFMRAKIATARFYADHLMPQIVGLEHTVCKGAPGV
                     MALSEAQF"
     gene            1749..>2154
                     /locus_tag="VFA35_00175"
     CDS             1749..>2154
                     /locus_tag="VFA35_00175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZT54611.1"
                     /translation="MTREVMEYDVVVVGGGPSGLACAIRLKQLAAERGREISVCLIEK
                     GAEVGAHILSGAVIEPRSLDELLPNWKELGAPLKTPVQDDRFLFLTAKRAVKLPTPPQ
                     MHNEGNYIVSLGNVCRWLAQQAEALGVEIYAGF"
BASE COUNT          376 a          749 c          744 g          285 t
ORIGIN      
        1 gcaggtcgcc gagctgccgg gctacgaaga ggcaggcccc gacacggccg aagccatcct
       61 cgacgaagcg gccaagttcg cctcgcaggt gctcgcgccg atgaaccgcg agggcgacac
      121 cgtcggctgc aagtggcgcg acggcgaggt gaccaccccg ccgggcaaca agcaggccta
      181 cgcgcagtac atcgcgggcg gctggaccgg gctggcgttt ccccagcaat acggcggcca
      241 gggcctgccc aaactgttgg ccaccgcggt acacgacatc gaacagtcgg ccaatctcgg
      301 cttctcgctg gccccgggac tgagccgcgg cgcgatcgaa gcgctgctgc tctgcggtac
      361 cgacgcgcag aaggaacgct atctgcaccg catgatcgca ggcgactgga ccgggaccat
      421 gaacctgacc gagccgcagg cgggctcgga cctcgggctg ctgcgcacca aggccgaacc
      481 cgacggcgac cactaccgcc tctacggcca gaagatcttc atctcgcacg gcgagcacga
      541 cctggccgag aacatcgtgc acctcgtgct ggcgcggctg cccgacgcgc ccgaaggcgt
      601 gcgcggcatc tcgctgttcg tcgcgccgaa gttcctcgtc aacgacgacg gaacgctcgg
      661 cgcacgcaac gacatccgct gcgtctcgat cgaacacaag atggggctgc acgcgagccc
      721 gacctgcgtg ctcgcctacg gcgaccgcga aggcgcgatc ggcaccctga tcggcgagcc
      781 gaaccgcggc ctcgaataca tgttcatcat gatgaacgag gcccgcttcg gggtcgggct
      841 gtcgggcatc gcgatcgccg agcgcgccta ccagcaggcc ctggcctacg ccaaggagcg
      901 cgtgcagtcc aaggacgtcg ccaacccgcg cggcccgtcg gtgacgatca tcaaccatcc
      961 ggacgtgcgc cgcatgctga tgacgatgaa agcgctgatc gagtcgacgc gcgccctgtg
     1021 ctacgtcgtc gccggggcga tggaccatgc ccatgcgtcg cccgacgagg ccgagcgcgc
     1081 ccgcgcccgc gccttcaccg agatcgtggt gccgatccac aaggcctggt cgaccgagat
     1141 ggcgctcgag gtgggcaacc tggcgatcca ggtgcacggc ggcatgggct tcatcgaaga
     1201 gaccggcgtc tcccagcaca tgcgcgacgc gcgcatcctg tcgatctacg agggcaccac
     1261 gggcatccag gccaacgacc tgatcgggcg caagatcgcg cgcgacggcg gggcgacggc
     1321 caaggccctg gccgagcaga tgaagggtgc gctggccgag ctgagcggca acgacgacga
     1381 cagccaggcg atccgcgcgc ggctggccga ttcgatcgcc accttcgagc gctgcgtcga
     1441 ctggatcgtc gccaacacca aggccgacgt gaaaggcgtg cacgccggtg cggtgccgtt
     1501 cctcaagctc gcggcgctca cctgcggcgg ctggcagatg gcgcgcgccg cggccgtcgc
     1561 gcagcgcaag ctcgcggcgg gcgagggcga cgccgagttc atgcgcgcca agatcgccac
     1621 cgcgcgcttc tacgccgacc acctgatgcc gcagatcgtc ggactcgagc acaccgtgtg
     1681 caagggcgcg cccggcgtga tggcgctcag cgaagcacag ttctgaccgt ctcgcgaagg
     1741 gccacacgat gacacgcgag gtgatggagt acgacgtggt cgtcgtcggc ggcgggccgt
     1801 cggggctggc gtgcgcgatc cggctgaagc agttggcggc cgagcgcggc cgcgagattt
     1861 cggtctgcct gatcgagaag ggcgccgagg tcggcgcgca catcctctcc ggcgcggtga
     1921 tcgagccgcg ttcgctggac gagctgctgc ccaactggaa ggagctgggt gcgccgctga
     1981 agacgccggt gcaggacgac cgcttcctgt tcctcaccgc gaagcgcgcc gtcaagctgc
     2041 cgacgccgcc gcaaatgcac aacgagggca actacatcgt cagcctcggc aacgtctgcc
     2101 gctggctggc gcagcaggcc gaagcgctgg gcgtcgagat ttacgccggc ttcg
//
LOCUS       DASRDG010000014         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_232734, whole genome shotgun sequence.
ACCESSION   DASRDG010000014 DASRDG010000000
VERSION     DASRDG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232734"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..434)
                     /locus_tag="VFA35_00180"
     CDS             complement(51..434)
                     /locus_tag="VFA35_00180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZT54612.1"
                     /translation="MAEIKIHNVDALGKPVGPYSHVARAKGGETLYIAGMLAPGESFE
                     AQCAAVFGQIEQALKSCGGGWKNVVQFTTYLVHSQDIAAFYRWREQNYPKMFPDGKYP
                     PNTLLIIDRLVQEQFLIEVQTVAAL"
     gene            complement(437..865)
                     /locus_tag="VFA35_00185"
     CDS             complement(437..865)
                     /locus_tag="VFA35_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZT54613.1"
                     /translation="MAPREDEALKPKHRVLVVDDEESTRLLLASMLSKDLGVDVQLAG
                     TCEQALRLAANYAYDAILLDLLMPGIGGQAVLAEIRQHTPNARTPVIIVSVIGDKSTV
                     DACMAAGANGYHHKPVRRADLVSTVKAQIAARGKPKPTTR"
     gene            complement(838..1392)
                     /locus_tag="VFA35_00190"
     CDS             complement(838..1392)
                     /locus_tag="VFA35_00190"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZT54614.1"
                     /translation="MGHRLSKITTRTGDAGETGLGDGSRVSKDSPRVQALGDVDELNS
                     AIGLVLAEEVPQAIRDLLLQVQQDLFDLGGELSIPGHKLLKPGRVDALESATESWNAE
                     LAPLKEFILPGGTRAAAAAHLARTVCRRAERSVVALGRKDKVGDSVRRYLNRLSDVLF
                     VAGRLLNRHAGQNDVQWRHEKTKR"
     gene            complement(1400..>2619)
                     /gene="xseA"
                     /locus_tag="VFA35_00195"
     CDS             complement(1400..>2619)
                     /gene="xseA"
                     /locus_tag="VFA35_00195"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZT54615.1"
                     /translation="FSPPAAPASPVVTVSELLRSVRDSLERRFPLAWVSGEISNCSRA
                     PSGHLYFTLKDGQAQVDCVMFRSRAALLQAELRDGARVEARARVTLYEPRGRFQLSVE
                     AVRQAGLGPLYERFLRVKEKLEREGLFAAALKRELPEFPRCIGVVTSLAAAALRDVLT
                     TLARRNPSLAVVVYPVPVQGQGAARQIAAMLGRASERADCDVLLLVRGGGSMEDLWPF
                     NEEVVARAIRQSGIPVVVGVGHETDFTIADFAADARAPTPTAAAEMASPSRAELAGRL
                     AACVQRLAREMRRRLEYAAQGLDGWVRRLVHPAARLAAHRDLVLQLNARLAFALAHCV
                     HRAEARLGRLRAALGGLDPAAVLSRGYSITYDAAGAVLRDAAKVKEGEALRTRLAQGE
                     LLSVVEGKKKPNL"
BASE COUNT          389 a          894 c          940 g          396 t
ORIGIN      
        1 gagcggaagc tttacgccga gctggtcagg cgctcaggcg ccaccgccga ctagagggcg
       61 gcgacggtct gcacctcgat caggaactgc tcctgcacga ggcgatcgat gatgagcagc
      121 gtgttcggcg gatacttgcc gtctggaaac atcttcgggt agttctgctc gcgccagcgg
      181 tagaacgcgg cgatgtcctg cgaatgcacg agataggtgg tgaactgcac cacgttcttc
      241 cacccgccgc cgcacgactt cagcgcctgc tcgatctgcc cgaagaccgc ggcgcactgc
      301 gcctcgaagc tctcgccggg agcgagcatg ccggcgatgt agagcgtctc gccgcctttg
      361 gcgcgcgcga cgtgggaata ggggccgacg ggcttgccga gcgcgtcgac gttgtggatc
      421 ttgatctcgg ccatcgctac ctcgtggtgg gcttgggctt gccgcgcgcc gcgatctgcg
      481 ccttgaccgt cgacaccagg tcggcacggc gcaccggctt gtggtgatag ccgttggcgc
      541 ccgccgccat gcaggcgtcg accgtcgact tgtcgccgat caccgagacg atgatgaccg
      601 gcgtgcgtgc gttcggcgtg tgctggcgga tctcggcgag caccgcctgg ccgccgatgc
      661 cgggcatcag caggtcgagc aggatcgcgt cgtaggcata gttggcggcg agccgcagcg
      721 cctgctcgca ggtgccggcg agctgcacgt cgacgcccag gtctttcgac agcatgctgg
      781 cgagcagcag gcgagtcgac tcttcgtcgt cgacgacgag cacccgatgt ttcggcttca
      841 gcgcttcgtc ttctcgtggc gccattgcac gtcattctgc cctgcgtggc gattgagcag
      901 gcgcccggcg acgaacagca cgtcggaaag ccggttcagg tagcgccgca cggaatcacc
      961 gaccttatcc ttgcgtccga gagcgaccac gctgcgttcc gcgcgccggc agacggtgcg
     1021 cgcgaggtgc gcggccgcgg cagctcgcgt gccgccggga aggatgaact ccttcagcgg
     1081 cgcgagctcg gcgttccagg attcggtggc gctttccagg gcgtcgacgc gcccgggctt
     1141 gagcagcttg tgaccgggaa tgctgagctc gccgccaagg tcgaacagat cctgctgcac
     1201 ctgcaacagc aggtcgcgaa tcgcctgcgg cacctcttcg gcgagcacca ggccgatggc
     1261 gctgttcagc tcgtcgacgt cgccgagcgc ctgaacgcgg ggactgtcct tcgagacccg
     1321 cgagccgtca ccgaggcccg tctcgccggc gtcgccggtg cgggtcgtga tcttcgacag
     1381 gcggtgcccc atgcgccgat tataggttcg gtttcttctt cccctcgaca acggaaagca
     1441 attcgccttg cgcgagtcgc gtgcgcagcg cttcaccttc cttgaccttc gccgcgtcgc
     1501 gcagcaccgc gccggcggcg tcgtaggtga tgctgtagcc gcgcgagagc acggcagcgg
     1561 ggtcgaggcc gccgagcgcg gcgcgcagcc gcccgaggcg cgcttccgcg cggtgcacgc
     1621 aatgcgcgag cgcgaaggcg aggcgggcgt tgagttgcag gacgaggtcg cggtgcgcgg
     1681 cgaggcgtgc ggccggatgc accaggcgtc gcacccagcc gtcgaggccc tgagcggcgt
     1741 attcgaggcg gcggcgcatc tcgcgcgcga ggcgctgcac gcaggccgcg aggcggccgg
     1801 cgagctcggc gcgcgacggg ctcgccatct ccgcggccgc ggtcggcgtc ggcgcgcgcg
     1861 cgtcggcggc gaagtcggcg atggtgaaat cggtctcgtg gccgacgccg accaccaccg
     1921 ggatgccgga ctgccggatt gcgcgggcaa cgacttcctc attgaacggc cacaggtcct
     1981 ccatcgagcc gccgccgcgc accagcagca gcacgtcgca gtcggcgcgc tcgctggcgc
     2041 ggccgagcat cgccgcgatc tgccgcgccg cgccctgtcc ctgcaccggc acgggataga
     2101 ccaccacggc gagcgacggg ttgcgccgcg cgagcgtcgt caggacgtcg cgcagcgccg
     2161 cggcggcgag cgacgtgacc acgccgatgc agcgcggaaa ttccggcagc tcgcgcttca
     2221 gcgccgctgc gaacaagcct tcgcgctcga gtttctcctt gacgcgcagg aagcgctcgt
     2281 aaagcgggcc gaggccggcc tgacgcaccg cctcgacgct gagctggaag cgcccgcggg
     2341 gctcgtagag ggtgacgcgc gcccgggcct cgacccgcgc gccgtcgcgc agctcggcct
     2401 gcaggagcgc cgcacggctg cggaacatga cgcagtcgac ctgcgcctgg ccgtccttca
     2461 gcgtgaaata gaggtgcccg gagggcgccc gggaacagtt cgagatctcg ccgctcaccc
     2521 aggcgagcgg aaagcgccgc tcaaggctgt cgcgcacgct gcgcagcaat tcggagacgg
     2581 tgacaaccgg gctcgcgggg gctgcaggcg gtgagaaaa
//
LOCUS       DASRDG010000015         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1613464, whole genome shotgun sequence.
ACCESSION   DASRDG010000015 DASRDG010000000
VERSION     DASRDG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613464"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFA35_00200"
     CDS             <1..477
                     /locus_tag="VFA35_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HZT54616.1"
                     /translation="AERFYPCTSDAAARSASLALIGDDLFAGGVWQAARAHARVAPTW
                     VYHFDHPQPFSAGQRFSEAEDPAALGVFHSAEYPYVFGSTAVLTRDWGRADLRMTELM
                     QAYWLQFAKGGDPNRADLPRWPTFVDAGSAPTVMRLAPEPGLIDVPRREPLAFAAT"
     gene            503..1336
                     /locus_tag="VFA35_00205"
     CDS             503..1336
                     /locus_tag="VFA35_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZT54617.1"
                     /translation="MISGKTTLIAHLGYPTEAFKAPMIYNPWFEQRGIDAVVVPVGVK
                     PEDYPATLAALASLTNLRGALVTMPHKVTTLGLVDKATPTARIAGACNALLKRDDGRW
                     LGDQFDGAGFVRGVERNGRRIAGARAFVAGCGGVGSAIAASLAAAGAAAIDLFDAQGA
                     SAEALARRLLEHYPELAVRTGSQDPAGHEIVVNATPLGMKEGDALPFDIARVDAGALV
                     GEVVMKAEHTPLLRAALARGLEVQVGSDMLFEMIPAYLEFFGFGTATADELRAVAQLR
                     Y"
     gene            1348..>2282
                     /locus_tag="VFA35_00210"
     CDS             1348..>2282
                     /locus_tag="VFA35_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZT54618.1"
                     /translation="MRRSIATVSMSGTLRQKLEAIAAARFDGIELFENDFIAFNGSAR
                     ELRTMAEDLGLGIDLFQPFRDFEGMPDEAFRKSLDRAERKFDLMQVLGAPLMLVCSNT
                     SALAVDDPARAAAQLHALAERAATRNLRIGYEALAWGRHVRLYGQAWKIVAAADHPHL
                     GLILDSFHTLSLQDDPAGIAAIPGERIFFLQMADAPLLAMDVLQWARHHRNFPGQGQF
                     DLENFFLQVLLSGYAGPLSLEIFNDQFRETPNRRTAVDAMRSLLFLESQARGRLERER
                     DAGAQGPAGELAARTLQRTPLFDAPRAPALTGFAFL"
BASE COUNT          320 a          872 c          753 g          337 t
ORIGIN      
        1 gccgagcgct tctatccgtg cacgagcgac gcggccgcgc gcagcgcgag cctggcgctg
       61 atcggcgacg acctgttcgc cggcggtgtc tggcaggccg cacgcgccca cgcccgcgtc
      121 gcgccgacct gggtctacca cttcgaccat ccccagccct tctctgccgg gcaacgcttc
      181 agcgaggccg aggatccggc cgcgctcggc gtcttccaca gcgccgagta cccctatgtc
      241 ttcggcagca ccgcggtgct gacgcgcgac tggggccgcg ccgatttgcg catgaccgag
      301 ctgatgcagg cctactggct gcagttcgcc aagggcggcg atcccaaccg cgcggacctg
      361 ccgcgctggc cgaccttcgt cgacgcaggc agcgctccca ccgtgatgcg tctcgcgccc
      421 gagcccgggc tgatcgacgt gccgcggcgc gagccgctcg ccttcgccgc cacctgaacc
      481 tccgccgaag acccaccgac ccatgatcag cggcaagacc accttgatcg cccacctcgg
      541 ctacccgacc gaggcgttca aggcacccat gatctacaac ccctggttcg agcagcgcgg
      601 catcgacgcc gtggtcgtgc cggtcggcgt caagcccgag gactacccgg cgacgctcgc
      661 ggcgctcgcc tccttgacca acctgcgcgg cgccctggtg accatgccgc acaaggtgac
      721 gacgctcggc ctggtcgaca aggcgacgcc gaccgcgcgc atcgcgggcg cctgcaatgc
      781 gttgctcaaa cgcgacgacg gccgctggct cggcgaccag ttcgacggcg ccggcttcgt
      841 tcgcggcgtc gagcgcaacg gccggcgcat cgccggggca agagccttcg ttgccggctg
      901 cggcggtgtc ggctcggcga tcgccgcctc gctcgccgcc gcgggcgcgg ccgcgatcga
      961 tctcttcgac gctcagggcg cgtcggccga ggccttggcc cggcgactgc tcgagcacta
     1021 ccccgagctc gccgtgcgca ccggatcgca ggacccggcc ggccacgaga tcgtcgtcaa
     1081 cgccacgccg ctcggcatga aggaaggcga cgccctgcct ttcgacatcg cccgcgtcga
     1141 cgccggggcg ctggtcggcg aggtcgtgat gaaggccgag cacacgccgc tgctgcgcgc
     1201 ggcgctggcg cgcggcctgg aggtgcaggt cggctccgac atgctgttcg agatgatccc
     1261 cgcctacctc gagttcttcg gcttcggcac cgccaccgcc gacgagctgc gcgccgtggc
     1321 gcagctccgc tattaaggag tacgacgatg cgccggtcga ttgccaccgt gtcgatgagc
     1381 ggcaccctgc gccagaagct ggaggcgatc gccgcggcgc gcttcgacgg catcgagctg
     1441 ttcgagaacg acttcatcgc cttcaacggc agtgcccgcg agctgcgcac catggccgag
     1501 gacctcggcc tcggcatcga cctgttccag ccctttcgcg atttcgaagg catgcccgac
     1561 gaagccttcc ggaagagcct ggaccgcgcc gagcgcaagt tcgacctgat gcaggtgctg
     1621 ggcgcgccgc tgatgctggt ctgctccaac acctcggcgc tggccgtcga cgatcccgcg
     1681 cgcgcggcgg cgcagctgca cgccctcgcc gagcgcgcgg ccacgcggaa cctgcgcatc
     1741 ggctacgagg cgctcgcctg gggccgccac gtgcgcctct atggccaggc ctggaaaata
     1801 gtcgccgcgg ccgaccatcc gcacctcggt ctcatcctcg acagcttcca cacgctctcg
     1861 ctccaggacg accccgccgg catcgccgcg atcccgggcg agcgcatctt cttcctgcag
     1921 atggccgacg cgccgctgct ggcgatggac gtgctgcagt gggcacggca ccaccgcaac
     1981 ttccccggcc agggccagtt cgacctggag aacttctttc tccaggtgct gctttcgggc
     2041 tacgccgggc cgctctcgct cgagatattc aacgaccagt tccgcgagac gccgaaccgg
     2101 cgcaccgccg tcgacgcgat gcgttcgctg ctctttctcg agagtcaggc gcgcgggcga
     2161 ctcgagcgcg agcgcgacgc cggggcgcaa ggcccggcgg gcgagctcgc ggcacggacg
     2221 ctgcagcgta ccccgctctt cgacgcgccg cgggcgccgg ccctgaccgg cttcgccttc
     2281 ct
//
LOCUS       DASRDG010000016         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1613480, whole genome shotgun sequence.
ACCESSION   DASRDG010000016 DASRDG010000000
VERSION     DASRDG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613480"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..600)
                     /locus_tag="VFA35_00215"
     CDS             complement(205..600)
                     /locus_tag="VFA35_00215"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HZT54619.1"
                     /translation="MLSRFTFHGGRRTVARRAEDDRATFVDLYGGRLFTVALAIIALN
                     LLDAWFTLLFLAQGGQEVNPFVQRVLNLGPSAFIAFKTFGIGACVMFLAITKNFLAAR
                     IGLVTVLLGYTLLLGWHLYLLVHLDLGAL"
     gene            835..1989
                     /locus_tag="VFA35_00220"
     CDS             835..1989
                     /locus_tag="VFA35_00220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HZT54620.1"
                     /translation="MAIPELQLKVRVRGRHPWFYRKMVVKPAQPLPAGCVARVVDRDG
                     RMLGSGFFNPRTELALRMFADRAVEDPAAHFVQTLAGAVALREELLDLPAATNAWRLV
                     HGEGDGCPGLLLDRLGDVFVAQVHSLGMLNQMEPLGEWLRARVRGCRLLLQQDEDAVR
                     REGMERLPRPQPLPVEVHEHGIRYAVQAGGGHKTGFFADQRDNRRLVQTLARGRRVLD
                     LCCNAGGFALNAAAGGARSVLAVDLDEAMAAATAANAQQNRLRVETRHADAFDVLREL
                     RGGEHDLLVLDPPKWVHGRDEFTAGIARYRDLNRLALQKVASGGLIVTCSCSGSVSED
                     SFLRMLQDAAADAGVQARVLHLRGAGADHPVALQCPETRYLKVVVLQVER"
     gene            1986..2555
                     /locus_tag="VFA35_00225"
     CDS             1986..2555
                     /locus_tag="VFA35_00225"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZT54621.1"
                     /translation="MTAADERYRSALSPLPMAWEPGPERRAAVIAPIAEARGEDWLCF
                     TVRRADLREHAGQVSFPGGVDDGDADPAACALRELQEELGVDPATVLLFGHLGARISS
                     SRYRVHCLVGRIPWPDADRVARSEVERLLCLPLAQLTDLGRWQQTAPAPASDGRSYSA
                     SPHFRAGDDVIWGLTGRFAFAFAQALARG"
BASE COUNT          353 a          985 c          993 g          334 t
ORIGIN      
        1 cgccggcggc gacggtcgcc gcgccgtcgc ggccgcgcca tgccagccgt tcgagcgcgc
       61 gctcgatcgc cgcctcgggc gccgccgacc ggccgcggag ccacgaacgg cgcacgccga
      121 agatgccgca cacggagcgc agtcatgccg cgggcagcgg ccggccgcaa gcgccgcgat
      181 cggccggccg atcagggcgg gccgtcagag ggcgccgaga tcgaggtgga ccagcaggta
      241 gaggtgccag cccagcagga gcgtgtagcc gagcagcacc gtcacgagcc cgatgcgcgc
      301 cgcgaggaag ttcttggtga tcgcgaggaa catcacgcag gcgccgatgc cgaaggtctt
      361 gaaggcgatg aacgccgacg gtccgaggtt cagcacccgc tgcacgaacg ggttcacctc
      421 ctggccgccc tgcgcgagga acagcagcgt gaaccatgcg tcgagcaggt tcagcgcgat
      481 gatcgccagc gccaccgtga acaggcggcc gccgtacagg tcgacgaacg tcgcgcggtc
      541 gtcctcggcc cgccgcgcca ccgtacggcg gccgccatgg aacgtgaacc gcgacaacat
      601 cggggtcggg gcgtgtctgc ggtctccgaa acggcgttca ggcgcttcca gcaggggcgt
      661 cggcatgcag tggggatccg ttgcctagac agtactccgc tgacacgagt cctgtgcagc
      721 gggccggccg cgccggcggc cagcgggcgc tgccatcgag accgcgcgtg cccgtttctg
      781 ggaccgcagt gcagctcggt tgcgcggccg cgggcagacc gccatcctgc gcccgtggcg
      841 atccccgaac tgcagctcaa ggtgcgcgtg cgcggccgcc atccgtggtt ctaccgcaag
      901 atggtcgtga agcccgcgca gccgctgccc gccggctgcg tcgcgcgcgt cgtcgaccgc
      961 gacggtcgca tgctgggttc cggcttcttc aacccgcgca ccgaactcgc gctgcgcatg
     1021 ttcgccgacc gcgcggtcga ggatcccgcg gcgcacttcg tgcagacgct cgcgggcgcc
     1081 gtggcgctgc gcgaggaact gctcgatctg cccgccgcga cgaacgcgtg gcgcctcgtg
     1141 cacggcgaag gcgacggctg cccgggcctg ctgctcgacc ggctcggcga cgtgttcgtg
     1201 gcgcaggtgc acagcctcgg catgctgaac cagatggagc cgctcggcga atggctgcgc
     1261 gcgcgcgtgc gcggctgccg gctcctgttg cagcaggacg aggacgcggt gcggcgcgaa
     1321 ggcatggagc ggctgccgcg gccgcagccg ctcccggtcg aggtgcacga gcacggcatc
     1381 cgctacgccg tgcaggcggg cggcggccac aagaccggct tcttcgccga ccagcgcgac
     1441 aaccggcggc tcgtgcagac gctggcgcgc ggccggcgcg tgctcgacct ctgctgcaac
     1501 gccggcggct tcgcgctcaa cgccgccgct ggcggcgcgc gcagcgtgct cgcggtcgac
     1561 ctcgacgagg cgatggcagc ggcgacggcg gccaacgcgc agcagaaccg gctgcgggtc
     1621 gagacgcgcc acgccgacgc cttcgacgtg ctgcgcgagc tgcgcggcgg cgagcacgac
     1681 ctgctggtgc tcgatccgcc caagtgggtg cacggccgcg acgagttcac cgccggcatc
     1741 gcgcgctacc gcgacctcaa ccggctcgcg ctgcagaagg tcgcgagcgg cggcttgatc
     1801 gtcacctgct cgtgctcggg cagcgtcagc gaggacagct tcctgcgcat gctgcaggac
     1861 gcggccgccg acgcgggcgt gcaggcgcgc gtgctgcacc tgcgcggcgc cggcgccgac
     1921 catccggtgg cgctgcagtg cccggagacg cgctacctca aggtcgtcgt gctgcaggtg
     1981 gaacggtgac cgccgccgac gagcgctacc ggtcggcgct gtcgccgctg ccgatggcgt
     2041 gggagccggg ccccgaacgg cgcgcggccg tgatcgcgcc gatcgccgag gcgcgcggcg
     2101 aggactggct ctgcttcacg gtgcgccgcg cggatctccg cgagcatgcc ggccaggtct
     2161 cgttccccgg cggcgtcgac gacggcgacg ccgaccccgc cgcgtgcgcg ctgcgcgaac
     2221 tgcaggagga gctcggcgtc gacccggcga ccgtgctcct gttcggccac ctcggcgcgc
     2281 gcatcagctc gtcgcgctac cgcgtgcact gtctcgtcgg ccgcatcccg tggcccgacg
     2341 cggaccgcgt cgccaggagc gaggtcgagc ggctgctctg cctgccgctg gcgcagctca
     2401 cggacctcgg ccgctggcag cagaccgcgc cggcgcccgc gtccgacggc cgcagctact
     2461 cggcgagtcc gcacttccgc gccggcgacg acgtgatctg ggggctgacc ggccggttcg
     2521 cgttcgcgtt cgcgcaggcg ctcgcgcgcg gctgaagcct tgcgcggcgc gccgtgcggc
     2581 cgaggcgcct gcgtggatca gcggacgcac gcgcccgcgg gcgtgaactc gccgcccagc
     2641 accgccgcag catcgatcgc cagcc
//
LOCUS       DASRDG010000017         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_578447, whole genome shotgun sequence.
ACCESSION   DASRDG010000017 DASRDG010000000
VERSION     DASRDG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578447"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="VFA35_00230"
     CDS             complement(<1..1351)
                     /locus_tag="VFA35_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZT54622.1"
                     /translation="MTLRARSALFLGALALAAALFAAALWRGIPLQTNVLAMLPATER
                     DAVAEKVVAALSGAIGSRVLVVFSHADEAKAKAAAERFVAEAGAAGGFRGLTARLPDA
                     DPRVLHKLYAPNRFGLLADADREALASGSWRVRDSVLQQLVSPVGGAAGLPLAQDPFG
                     FFGRWMASLLPSAGTMRLEDGYLLSREGGRARVLVLGEVNGDVYDNAVQARAVAAFER
                     GAKAALAAEPGAEVLRTGTVFFAASARAASMRDMDRIAIGSIAGITLLMLAAFRSLRP
                     IVLGLLSAGAGILAATLALIAVDGELHLVTLAFGASLIGEAIDYAILFCAAHLAAGSR
                     WTPEMGVKQVRPALTVAVATSLLAYALLGLLPFPGVSQVARFALVGLAVAYLSVQWLL
                     PGLLVKPSRRDPEATTAWAARLLARWSAVLRGRRAVVVAALALLACIPGWLMLHPNDD
                     "
     gene            complement(1348..1947)
                     /locus_tag="VFA35_00235"
     CDS             complement(1348..1947)
                     /locus_tag="VFA35_00235"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HZT54623.1"
                     /translation="MRAAALLLLACAFPAQADELLGGILKRLGEPAVVRAQFVQERLV
                     TDMTRPTVSRGRIAVSRRDGLLWRIESPLKLTLAFTPTAIIETGPDGVRRLRGQGRGV
                     DTQIGRVLRGTLGADEPVLRESFEATASGSPERWTVRLAPRPREMARVLREIRLAGGR
                     HLESIEVEETAGNLTTIRIRQFSAAERLEPDELEFFKAP"
     gene            complement(1944..>2137)
                     /locus_tag="VFA35_00240"
     CDS             complement(1944..>2137)
                     /locus_tag="VFA35_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZT54624.1"
                     /translation="QVVLASAEVLEYENRLKIGYRITDRASGERLTKGWSVQVAIDAK
                     TRELQFVSPTILLDKMRGR"
BASE COUNT          320 a          779 c          785 g          253 t
ORIGIN      
        1 cgtcgtcgtt cggatgcagc atgagccagc cgggaatgca ggcgaggagc gcgagcgccg
       61 cgaccacgac cgcgcgccgt ccgcgcagta ccgcgctcca gcgcgcgagc aggcgcgccg
      121 cccaggcggt cgtcgcctcc gggtcgcggc gcgaaggctt gaccagcagg ccgggcagca
      181 gccattgcac gctgagatag gcgaccgcga ggcccaccag cgcgaagcgc gccacctggg
      241 agacgccggg gaagggaagc aagccgagca gcgcataggc gagaaggctc gtcgccaccg
      301 ctaccgtgag cgccgggcga acctgcttca cgcccatctc gggcgtccag cggctgccgg
      361 ccgcgaggtg cgcggcgcag aacaggatcg catagtcgat cgcctcgccg atcaggctcg
      421 cgccgaaggc gagggtgacg aggtgcagct cgccatcgac cgcaatcagc gcgagcgtcg
      481 cggcgaggat gcccgcgccg gcggagagca ggccgagcac gatcggccgc aacgagcgga
      541 acgcggcgag catgagcagc gtgatgccgg cgatcgagcc gatcgcgatg cggtccatgt
      601 cgcgcatcga cgcggcgcgc gcggatgcgg cgaagaacac cgtgccggtg cgcagcacct
      661 cggcgcccgg ctcggccgcg agcgccgcct tcgcgccgcg ctcgaacgcg gcgacggcac
      721 gcgcctgcac agcgttgtcg tagacgtcgc cgttgacttc gccgaggacg agcacccgag
      781 cgcggccgcc ttcgcgggag aggaggtagc cgtcttcgag ccgcatagtc ccggcgctcg
      841 ggaggagcga cgccatccag cggccgaaga agccgaacgg gtcctgcgcg agcggcaggc
      901 cggcggcgcc gcccaccggc gagacgagct gctgcagcac gctgtcgcgc acccgccagc
      961 tgcccgaggc gagcgcctcg cggtcggcat cggcgagcag cccgaagcgg ttcggcgcat
     1021 aaagcttgtg cagcacgcgc gggtcggcgt cgggcaggcg cgcggtcagg ccgcgaaaac
     1081 cgccagccgc gccggcttcg gcgacgaagc gctcggcggc ggccttcgct ttcgcctcgt
     1141 cggcatgcga gaagaccacc agcacccggc tgccgatcgc gccgctcagc gcggcgacca
     1201 ctttttccgc caccgcgtcg cgctcggtgg cgggcagcat cgccagcacg ttggtctgaa
     1261 gcgggatgcc gcgccacagc gcggcggcga aaagcgctgc ggcgagcgcg agcgcgccaa
     1321 ggaacagcgc cgagcgcgcg cgcagcgtca tggcgccttg aagaattcga gctcgtcggg
     1381 ctcgagccgc tcggccgccg agaactgccg tatgcggatg gtggtgagat tgcccgcggt
     1441 ttcctcgacc tcgatgctct cgagatggcg gccgccggcg aggcggatct cgcgcagcac
     1501 acgcgccatc tcgcgcggcc gcggcgcgag gcggacggtc cagcgctccg ggctgccgct
     1561 cgccgtcgcc tcgaagctct cgcgcagcac cggctcgtcg gcgccgaggg tgccgcgcag
     1621 cacccggccg atctgcgtgt cgacgccgcg gccctgcccg cgcagccgcc gtacgccgtc
     1681 cgggccggtc tcgatgatcg cggtcggcgt gaaggcgagc gtgagcttca gcggcgattc
     1741 gatccgccac agcagcccgt cgcggcgcga caccgcgatg cggccgcggc tcacggtcgg
     1801 ccgcgtcatg tcggtgacca ggcgctcctg cacgaactgc gcgcgcacca ccgccggctc
     1861 gccgaggcgc ttgaggatgc cgccgagcag ctcgtcggcc tgtgcgggaa acgcgcaggc
     1921 gagcagcagg agcgccgccg ccctcatcgt ccgcgcatct tgtcgagcag gatcgtcggc
     1981 gagacgaact gcagctcgcg cgtcttcgcg tcgatcgcca cctgcaccga ccagcccttg
     2041 gtcaggcgct cgccgctcgc gcggtcggtg atccggtagc cgatcttcag gcggttctcg
     2101 tattccagca cctcggcgct cgccagcacg acctggc
//
LOCUS       DASRDG010000018         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_234781, whole genome shotgun sequence.
ACCESSION   DASRDG010000018 DASRDG010000000
VERSION     DASRDG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234781"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..>2397
                     /locus_tag="VFA35_00245"
     CDS             337..>2397
                     /locus_tag="VFA35_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZT54625.1"
                     /translation="MPQSPDDSPAPATPAAPVTRRWPQRLGRLGRIGQWSALTAAVAV
                     AAVAVYLGVLVYTTPQVEDLRRAQNLRPSVILSSDGEVIGRFSTAYQAPVSLKDVPKD
                     LVNALIATEDHRFFEHSGIDPTRIVGSAWGIVLHGKMAGGSTLTQQLARNLFPQEIGN
                     EQSLNRKLREAITALRMERSSTKEQILESYLNSAPFLYNVRGIEMAARTYFDKPAARL
                     DTAQCATLVGMLKGAERYNPVRNPERARERRNLVMQQMVKHGTLAQARYEQLRARPLV
                     LAFKRPEEGDIGQARHFVAQLREQLVEWADAHDVDLDADGLVIRSTLDARLQRIAQDA
                     VVQQVALLQRIAGDEWSDARPRTARPRGAPASAKGEPAAGFAYFWKQHPEMLREMARD
                     TPQFRLATQAGRSDAQALDAVLADAATMARLRDERTRLSAGFIAIDPTSGAVRAWIGS
                     PDFAREQFDHVAQAKRQPGSTFKPFVYGAALQRGISTEHQYFDGAVDIVLADGSVWQP
                     TDGGGPSFAPMTLREGLVQSKNTITAQVMQEVGVDAVASFARAAGVRDSKLDLVPSLA
                     LGTSPVTLLEMGNAYATLAALGERRAPLLIAQIADRNGKVLDSFDAKPERVLDRAVVE
                     KLVDVMRGVVQTGTGAAIRSEWGVRGDLAGKTGTTQDNTDGWFMLISPTLVAGAWVGF
                     NDPRV"
BASE COUNT          381 a          873 c          843 g          300 t
ORIGIN      
        1 ttgccttcga ccggcccacc ctgcgccgcg gccggcgcgg cgacgacgaa cagcgaggag
       61 gcggcaaggc cggtgagggc gagctgggcg gagaagtcgc gacggttcac gttcgagttc
      121 cttgtgatgg tgtcgccgac agagtggcgt cggcgctgaa aagttcaggc ggtctgcacg
      181 acaacgcggc aacggcgcaa gccgccgacc ggcgcggccc gaaaatcggc atgggcgcga
      241 ttgtccgaca aacaccgcgc cacaccctgt cacagcgcgg gcgccgcccg tgcgacgatt
      301 ccacgacgac cctccgtcaa cgcttccaac ccccgcatgc cgcaatctcc cgacgactcc
      361 cccgcgcccg ccacgcccgc tgcgcctgtc acacgccgct ggccgcagcg cctcgggcgc
      421 ctcgggcgca ttggtcaatg gtccgcgctg actgccgccg tcgcggtcgc cgccgtcgcc
      481 gtctacctgg gcgtgctggt ctacaccacg ccgcaggtcg aggatctgcg gcgcgcgcag
      541 aacctgcggc cgagcgtgat cctgtcgagc gacggtgagg tgatcggccg tttctcgacc
      601 gcctaccagg cgccggtgag cctgaaggac gtgccgaagg acctggtgaa cgcgctgatc
      661 gcgaccgagg accaccgctt cttcgagcac agcgggatcg acccgacgcg catcgtcggc
      721 tcggcctggg gcatcgtgct gcacggcaag atggccggcg gctcgacgct gacgcagcag
      781 ctcgcgcgca acctgttccc gcaggagatc ggcaacgagc agagcctgaa ccgcaagctg
      841 cgcgaggcga tcaccgcgct gcgcatggaa cgcagcagca ccaaggagca gatcctcgag
      901 agctacctca acagcgcgcc gttcctctac aacgtgcgcg gcatcgagat ggccgcgcgc
      961 acctacttcg acaagccggc cgcgcggctc gacaccgcgc agtgcgccac gctggtgggc
     1021 atgctcaagg gcgcggagcg ctacaacccg gtgcgcaacc ccgagcgcgc gcgcgagcgg
     1081 cgcaacctcg tgatgcagca gatggtcaag cacggcacgc tggcgcaggc gcgctacgag
     1141 caattgcgcg cgcggccgct ggtgctggcg ttcaagcgcc ccgaggaagg cgacatcggc
     1201 caggcgcgcc acttcgtcgc gcagctgcgc gagcaactgg tcgaatgggc cgacgcgcac
     1261 gatgtcgatc tcgacgccga cggcctggtc atccgcagca cgctcgacgc ccggctgcag
     1321 cgcatcgcgc aggacgcggt ggtacagcag gtcgcgctgc tgcagcgcat cgccggcgac
     1381 gagtggagcg atgcccggcc gcgcaccgcg cgcccgcggg gcgcgccggc gtcggccaaa
     1441 ggcgagcccg ccgccgggtt cgcgtatttc tggaagcagc accccgagat gctgcgcgag
     1501 atggcgcgcg acacgccgca gttccgcctt gccacgcagg ccggccgcag cgacgcgcag
     1561 gcgctcgacg cggtgctcgc cgacgccgcc acgatggcca ggctgcgcga cgagcgcacg
     1621 cgcctgtcgg ccggcttcat cgcgatcgac ccgaccagcg gcgcggtgcg cgcctggatc
     1681 ggcagccccg acttcgcgcg cgagcagttc gaccacgtcg cacaggcgaa gcgccagccc
     1741 ggctcgacct tcaagccctt cgtctacggc gcggcgctgc agcgcggcat ctcgaccgag
     1801 caccaatact tcgacggcgc ggtcgacatc gtgctggccg acggcagcgt ctggcagccg
     1861 accgacggcg gcggcccgag ctttgcgccg atgacgctgc gcgaaggtct ggtgcagtcg
     1921 aagaacacga tcaccgcgca ggtcatgcag gaggtcggcg tcgacgcggt cgcgagcttc
     1981 gcgcgggcgg ccggcgtgcg cgactcgaag ctcgacctgg tgccctcgct cgcgctcggc
     2041 accagcccgg tcacgctgct cgagatgggc aacgcctacg cgacgctcgc ggcgctcggc
     2101 gagcgccgcg cgccgttgct gatcgcgcag atcgccgacc gcaacggcaa ggtgctcgac
     2161 agcttcgacg ccaagcccga gcgcgtgctc gaccgcgcgg tggtcgagaa gctcgtcgac
     2221 gtgatgcgcg gcgtggtgca gaccggcacc ggcgcggcga tccgcagcga gtggggcgtg
     2281 cgcggcgatc tcgccggcaa gaccggcacc acgcaggaca acaccgacgg ctggttcatg
     2341 ctgatcagcc cgacgctggt ggccggcgcc tgggtcggct tcaacgaccc gcgcgtg
//
LOCUS       DASRDG010000019         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1384269, whole genome shotgun sequence.
ACCESSION   DASRDG010000019 DASRDG010000000
VERSION     DASRDG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384269"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..868
                     /locus_tag="VFA35_00250"
     CDS             92..868
                     /locus_tag="VFA35_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT54626.1"
                     /translation="MSAVDAPAPLLEVSRLNRHFGGLHAVKNVSFSVQRGEIVGVLGP
                     NGAGKTTLYNLLTGFIPPDAGASIRFEGRSLLGLAPHRIAGLGISRTFQLCRPFVGMS
                     VAENVIVGALGARRRGVTDHEELAAGLLRRVGLAGKDKLPVEVLSYGDQRRLEIARAL
                     AARPALLLLDEPFAGLGSGEIEDLSTLIRQVHAEEGLTVVLIEHKLREFMRLVSRVIA
                     LDFGEVIAEGTPEAIVQHPAVIEAYIGRGNVDPLEAGRAA"
     gene            858..1565
                     /locus_tag="VFA35_00255"
     CDS             858..1565
                     /locus_tag="VFA35_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT54627.1"
                     /translation="MLLELRELSVSYGKAVALEKVTLQVDAGEFVAVLGPNGAGKSTL
                     LKAISRAQPSTGALNLRGESLQGLPAHAVVGKGISHCPEGRRLFPELTVLKNLMLGAY
                     LRRDAAAVAADLEQVYGLFPILRDRGQQVVSTLSGGQQQMVAIGRALMGRPALLLLDE
                     PSVGIAHRLKIEIFDVIKRIQRGGTAILMAEQDAQSALRIADRVYVLEHGHVGREGTA
                     AELGADDYIRQAYLGVG"
     gene            complement(1581..1979)
                     /locus_tag="VFA35_00260"
                     /pseudo
     CDS             complement(1581..1979)
                     /locus_tag="VFA35_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
     gene            1974..2123
                     /locus_tag="VFA35_00265"
                     /pseudo
     CDS             1974..2123
                     /locus_tag="VFA35_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027724.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
BASE COUNT          287 a          700 c          775 g          361 t
ORIGIN      
        1 gctgcgtgct gatcgccatc ctcttcttcg tgccgggcgg gctggtggcc ccgacctggc
       61 ggcgcatcaa ggccgcacgc ggctggggac catgagtgcc gtggacgccc cggcaccgct
      121 gctggaggtg agcaggctca accgccattt cggcggtctg cacgcggtga agaacgtgtc
      181 gttcagcgtg cagcgcggcg agatcgtggg cgtgctgggt cccaacggcg ccggcaagac
      241 cacgctgtac aacctgttga ccggcttcat cccgcccgac gccggcgcca gcatccgctt
      301 cgaaggtcgc tcgctgctgg gccttgcgcc gcatcgcatc gccgggctcg ggatctcgcg
      361 caccttccag ctgtgccggc cgttcgtcgg catgagcgtg gccgagaacg tgatcgtcgg
      421 cgcgctcggt gcgcgtcgcc gcggcgtcac cgaccacgag gagttggctg cagggctgct
      481 gcggcgcgtc ggtctggccg gcaaggacaa gctcccggtg gaggtgctgt cgtacggcga
      541 ccagcgccgg ctcgagatcg cgcgcgccct ggccgcgcgc ccggcgctgc tgctgctcga
      601 tgaaccgttc gccggcctgg gcagcggcga gatcgaagac ctgtcgacgc tgatccgaca
      661 ggtgcatgcc gaagagggcc tgacggtggt gctgatcgaa cacaagctgc gcgagttcat
      721 gcgtctggtc agtcgcgtga tcgccctcga tttcggcgag gtgatcgccg agggcacgcc
      781 cgaggcgatc gtgcagcacc cggcggtgat cgaggcctac atcggccgcg gcaacgtcga
      841 cccgctggag gccggccgtg ctgcttgagt tgcgtgagtt gtcggtgagc tacggcaagg
      901 cggtggcgct ggagaaggtg acgctgcagg tcgacgcggg cgagttcgtc gcggtgctgg
      961 gccccaacgg cgccggcaag agcacgctgc tgaaggcgat ttcgcgcgcg cagccgtcga
     1021 ccggcgcgct gaacctgcgc ggcgagtcat tgcaaggcct gccggcgcat gcggtggtcg
     1081 gcaagggcat cagccactgc cccgagggcc gtcgattgtt ccccgagctg acggtgctga
     1141 agaacctgat gctgggcgcc tacctgcggc gcgatgcagc cgcggtggcg gccgacctgg
     1201 agcaggtcta cggcctgttc ccgatcctgc gcgaccgcgg gcagcaggtc gtgagcacgc
     1261 tgtcgggcgg ccagcagcag atggtggcga tcgggcgcgc gctgatgggc aggccggcgc
     1321 tgctgctgct cgacgaaccg tcggtgggca tcgcgcaccg gttgaagatc gagatcttcg
     1381 acgtcatcaa gcgcatccag cgcggcggca cggcgatcct gatggccgag caggacgcgc
     1441 agtcggcgct tcgcatcgcc gaccgggtct acgtgctgga gcatggccac gtcggccgcg
     1501 aagggactgc cgccgagctg ggcgccgacg actacatccg gcaggcctac ctcggggtgg
     1561 gctgaggcgc cggccgctag tcagggctcc ggctccggaa agcgccgttt gaccgattcg
     1621 agcagcggcg cgacgatgtc catcggcagc ggaaagacga tggtcgagtt cttgtccgcg
     1681 gcgatcaccg tcagcgtctc caggtagcgc agctggatcg cctgtggctg tcgcgccagg
     1741 atctccgcgg cctgggcgag cttttcggcg gcctgcagct cgccctcggc atggatcacc
     1801 ttggcgcgcc gctcccgctc ggcctcggcc tggcgcgcaa tcgcccggat catcgactcg
     1861 ttgaggtcga cgtgcttgat ctcgacattg gtgaccttga tgccccaagc atcggtctgg
     1921 ctgtcgagga tcttctgcag gtcgaggttg agccgctctc tctccgagag catctcgtcg
     1981 gcgaggcctt cttgccgagc tacggggcgc tcggcatcgg cggggtcgcg gcgttcgcgt
     2041 tcggtgcgct cctcctgatc gacagcgacc tgccggggtt cggcatcccg ccctcgctcg
     2101 tcgtcgtcgc gacgctcgtt tcg
//
LOCUS       DASRDG010000020         3411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1384652, whole genome shotgun sequence.
ACCESSION   DASRDG010000020 DASRDG010000000
VERSION     DASRDG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384652"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFA35_00270"
     CDS             <1..358
                     /locus_tag="VFA35_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZT54628.1"
                     /translation="GAVGFVPKSTPPAEIINAIRAVLDGERWLPPALAQALDSAQSDP
                     ADAELAAHLSRLTGQQFRVLAQIGEGRLNKQIADSLGIQERTVKAHVSAIFEKLGVRN
                     RTQASVLLQRLRLQQK"
     gene            complement(365..>3411)
                     /locus_tag="VFA35_00275"
     CDS             complement(365..>3411)
                     /locus_tag="VFA35_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HZT54629.1"
                     /translation="RSPALSALVTAVMLLGMLPYIALQLKAVAMSFTTILGDPRGLTA
                     WRDGALWVAVSMALFAIVFGTRRSSAVAHNRGLVLAIAFESLFKLVAMLALTSLLFMA
                     PIPGEAAPPPTHDLTGFPSLILLGALAMFTLPHQFHAGVVECRDVRHLKTARWLFPLY
                     MVLISVPALPLARLGDAQLAARGVPSDLYLLALPLAHGEKALALLAFLGSLSASTCMV
                     VVATLALSLMVANHFIAPLLLRAGWGQNEDNDLRGEVLWQRRAAILAIIALAWAYSRV
                     IFDEAALADIGALSFSALGGLAPTTWAAVYRPQLGARAVFAGLAAGTLVWMYTLLPGV
                     IAPHAAWLSTGPFGISALAPTHLFGLDGFGALTRAVVLGIATNLIVIAMVELFGARRA
                     APATGVLLDNRALRALAMRFLSEERAQRLVGDVSANKPADAATLAAVEHELSAVIGAS
                     SARLLLDVARRDRRMQLDTVAAIVDEASADLRFNQRVLLAALENMSQGICVVDAELHV
                     VAWNRRYAELFLYPPGLLKVGRSVEELARYSIGAGMIGTDTRDPERLEAAVQRRMAHM
                     RAGTPHLSERRFADGSMVEIRGNPMPGGGFVATFTDVTEFRRAALALERANETLELRV
                     AERTAELERATHAAETANETKTRFLAAVSHDLMQPLHAAQLFAHSLAARTDDDGRVTA
                     RHLHSSLTATESLLGGLLDMARLDAGKLVPRPRAFALNELLGTLAAEFAVLASEKGLR
                     LHHVPTRAWVRSDPHLLRRVLQNFLSNALRHVESGRVLLGCRRAGAELRIEVWDTGPG
                     IAESETRLIFEEFRRGSRASNQGLGLGLAIAQRLARLLDHDLSLRSWAGHGSVFAVSV
                     PRSAAAYAAIAASAPAQEAGPPADMPKGTALIVDNEPQALIALRTLLEQWGWRVLTAP
                     DAVKALDLARSERPDIAILDYHLAGGDNGLDLYTKLQIVLGDIPAAILTADRDAALRQ
                     RTLELGLMLLYKPLKPLALKQVLRKSEGAGVRFVASSLTPQG"
BASE COUNT          552 a         1134 c         1240 g          485 t
ORIGIN      
        1 cggtgcggtc ggcttcgtgc cgaagagcac gccgccggcg gaaatcatca acgcgatccg
       61 cgccgtgctc gacggcgaac gctggttgcc gcccgcactg gcgcaagcac tcgattcggc
      121 gcagagcgac ccggccgacg ccgagctcgc cgcgcacctt tcgcgcctga ccggccagca
      181 gttccgcgtg ctcgcgcaga tcggcgaagg ccgcctcaac aagcagatcg ccgactcgct
      241 cggcatccag gagcgcacgg tcaaggctca tgtcagcgcg atcttcgaga agctcggcgt
      301 gcgcaaccgc acccaggcga gcgtgctgct gcagcggctg cgtctgcagc agaaatgacg
      361 cgtttcatcc ttgcggggtg agcgaggagg cgacgaaacg cacccccgcg ccctcgctct
      421 tgcgcaacac ctgcttcagc gcgagcggtt tgagcggctt gtacagcagc atgaggccga
      481 gttcgagcgt gcgctggcgc agtgccgcat cgcgatcggc ggtaaggatg gcggccggga
      541 tgtcgccgag gacgatctgc aactttgtat acaaatccag tccgttgtcg ccaccggcga
      601 ggtggtagtc gaggatggcg atgtcgggcc gctcgctgcg cgcgagatcg agtgccttca
      661 ccgcgtcagg tgcggtgagc acgcgccagc cccattgttc gagcagtgtg cgcagcgcga
      721 tcagtgcctg cggttcgttg tcgacgatca aagccgtgcc tttcggcatg tccgctggcg
      781 gcccagcttc ctgtgccgga gccgaagccg cgatcgccgc ataggcggcc gcgctgcgcg
      841 gtacgcttac cgcgaacaca ctgccgtggc cggcccagga gcgcaggctc aggtcgtggt
      901 cgagcaggcg cgccagacgc tgggcaatgg ccaggcccaa ccccaggccc tggttgctcg
      961 cgcggctgcc gcggcggaat tcctcgaaga tcagtcgtgt ctcgctttcg gcgatgccgg
     1021 ggccggtgtc ccagacctcg atgcgcagct ccgcgcccgc gcgccggcag ccgagcagga
     1081 cgcgtcccga ttcgacatgg cgcagcgcgt tggaaaggaa gttctgcagc acgcggcgca
     1141 gcaggtgcgg atccgagcgc acccacgcgc gcgtcggtac atgatgcagg cgcaatcctt
     1201 tctcgctcgc gagcacggcg aactcggccg caagcgtgcc gagcagttcg ttgagcgcga
     1261 acgcgcgcgg acgcggcacg agcttgccgg cgtcgagccg cgccatgtcg agcaggccgc
     1321 cgagcagcga ctcggtcgcg gtcagcgagc tgtgcaggtg gcgcgcggtg acgcggccgt
     1381 cgtcgtcggt acgcgcggcg aggctgtgcg cgaacagttg cgcggcgtgc agcggctgca
     1441 tgaggtcgtg gctgaccgcg gcgaggaagc gcgtcttggt ctcgttcgcg gtttcggcgg
     1501 catgcgtggc gcgttcgagt tcggcggtgc gctcggcgac gcgcagttcg agcgtctcgt
     1561 tcgcgcgttc gagcgcgagt gcggcgcggc ggaattcggt gacgtcggtg aacgtggcga
     1621 cgaagccgcc gccgggcatc ggattgccgc ggatctcgac catgctgccg tccgcaaagc
     1681 gccgctccga cagatgcggt gtgcccgcac gcatgtgcgc catgcgtcgt tgcacggcgg
     1741 cctcgagtcg ttcgggatcg cgcgtgtcgg tgcctatcat gcccgcgccg atgctgtagc
     1801 gcgccagttc ctcgacggag cggcccacct tgagcagccc gggcggatag aggaatagtt
     1861 cggcatagcg ccggttccag gcgaccacat gcagttcggc gtcgacgacg cagatgccct
     1921 gcgacatgtt ctcgagcgcg gcgagcagca cgcgctggtt gaagcgcagg tcggccgaag
     1981 cctcgtcgac gattgccgcg accgtgtcga gctgcatgcg ccggtcacgg cgcgcgacgt
     2041 cgagcagcag gcgtgcgctc gatgcgccga tcaccgccga cagctcgtgc tcgaccgcgg
     2101 caagcgtggc agcgtcggcc ggcttgttcg cggacacgtc gccgaccagg cgctgtgcgc
     2161 gctcctcgga caggaagcgc atcgccagcg cgcgcaacgc gcggttatcg agcagcacgc
     2221 cggttgccgg tgcggcccgg cgcgcgccga acagttcgac catcgcgatg acgatcaggt
     2281 tggtggcgat gccgaggacc accgcgcgcg tcagtgcgcc gaagccgtcg agaccgaaca
     2341 ggtgcgtcgg cgcaagcgcg ctgataccga atggtcccgt cgacagccac gccgcatgcg
     2401 gcgcgatcac gccgggcagc agcgtgtaca tccataccag cgtgcccgcg gcgagtccgg
     2461 cgaacaccgc gcgcgcgccg agttgcgggc ggtacactgc cgcccaggtc gtcggcgcga
     2521 gtccgccgag cgcggagaac gacagcgcac cgatgtcggc cagcgcggcc tcgtcgaaga
     2581 tcacgcggct gtaggcccag gccagggcga tgatggcgag gatcgccgcg cggcgctgcc
     2641 acagcacttc gccgcgcaga tcgttgtcct cgttctggcc ccagccggcg cgcagcagca
     2701 atggcgcgat gaaatgattg gcgaccatca ggctcagcgc gagcgtggcg acgacgacca
     2761 tgcaggtcga ggccgacagg ctgccgagaa aggcgagcag ggcgagcgct ttctcgccat
     2821 gcgcgagcgg cagcgcgagc aggtacaggt ccgagggcac gccgcgcgcg gcgagctgcg
     2881 catcgccgag gcgcgcgagc ggcagcgccg gcaccgagat cagcaccatg tacagcggga
     2941 acagccagcg cgcggttttc agatggcgca catcgcggca ttcgaccacg ccggcatgga
     3001 actggtgcgg cagggtgaac atcgccagcg cgccgagcag gatcaggctc ggaaaaccgg
     3061 tgagatcgtg cgtgggcggc ggcgcggctt cgccgggtat cggcgccatg aacagcaggc
     3121 tggtcagggc gagcatggcg acgagcttga acagcgattc gaacgcgatc gcgaggacga
     3181 ggccgcggtt gtgcgcgacc gcggacgatc gccgcgtgcc gaacacgatc gcgaacagcg
     3241 ccatcgacac ggcgacccag agcgcgccgt cgcgccacgc ggtcaggcca cgcggatcgc
     3301 cgaggatcgt ggtgaagctc atcgcgacgg ctttgagctg cagcgcgatg tagggcagca
     3361 tgccaagcag catcaccgcg gtgaccagcg cgctcagcgc ggggctcctg c
//
LOCUS       DASRDG010000021         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1384924, whole genome shotgun sequence.
ACCESSION   DASRDG010000021 DASRDG010000000
VERSION     DASRDG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384924"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VFA35_00280"
     CDS             <1..1140
                     /locus_tag="VFA35_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HZT54630.1"
                     /translation="AARAIAHQIVEWVGGERAILVVVLACALLTYGGVSLFVVAFSVY
                     PIAASLFCEAQIPKRLIPAAIGLGSFTFTMVALPGTPAIQNAIPIPFFKTTIYAAPGM
                     GLIAAVFMFAVGMLWLTRRSHAARKSGEGYGEHKEEFTAVDNSNLPSFFMALLPILAV
                     IGLNFALSKYVIPAFDHSYLDTAKFKHIELKKVLAIWAIIGAMAAAIVVLALTNRGRI
                     ADLRDAFNKGTLGSLLPMANTASEVGYGAVIASLATFAVVREGLKSVASDPVVSLWLV
                     VTVLAAMTGSASGGMSLALGAMGEYYAHAAEAAKINPEIMHRIAAMGSAAMDILPHNG
                     AVITLLAICGLTHKQSYFDLFVVGSLTAFSASILVLGLYLTVGVF"
     gene            1140..1934
                     /locus_tag="VFA35_00285"
     CDS             1140..1934
                     /locus_tag="VFA35_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54631.1"
                     /translation="MLRSVLMMGLALASATAPAQTLRLLAAELPPYTYQVPSASVAEQ
                     PGPGRGVIHELVAEAARRVGHSGRIEYLPWYRAQEIAQTEGFVGILALTRSPEREDKY
                     RWLLHLMDDDLVLVGSPGVDVSDLSKVRDRPVGVLQRSGAEALLRSLGFARVSPQPEE
                     WMNAKRIKDRSINAWVAPRAMVVHAVREVRGNLDTLQFGQVVRTSELWLATSKSLPDA
                     EAKKWQDAWAAMRADGSAERIVRRNLNPRVDPIEDAKRLIQEEPFR"
     gene            1959..>2941
                     /locus_tag="VFA35_00290"
     CDS             1959..>2941
                     /locus_tag="VFA35_00290"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZT54632.1"
                     /translation="MHSRHTALAALTQTQRGKVVSAHDAVRLIRDGDTVATGGFVGIG
                     FAEEIALALEALYLETDTADAQSTGKPRNLTLVYAAGQGDGKHKGLNHFAHEGLVKRV
                     IGGHWGLCPKLQQMAAGNRIEAYNLPQGVISHLFRDIAGGKPGHLSSVGLGTFVDPRF
                     GGGKLNARTTQDIVRLMPIDGRDYLFYQAFPIQVGIIRGTTADPDGNITMEREALTLE
                     ALAIAMAVHNSGGIVIVQVERLAARHALSAREVKVPGALVDCVVVARPENHWQTFVEP
                     YSAAFSGELRVPQDSAGPTAPMPLNERKIIARRAALELDANSVVNLGIGMP"
BASE COUNT          456 a         1043 c          997 g          445 t
ORIGIN      
        1 gcggcgcgcg cgatcgcgca ccagatcgtc gagtgggtcg gcggcgaacg cgcgatcctc
       61 gtggtcgtgc tggcgtgtgc gctgctgacc tacggcggcg tgtcgctgtt cgtcgtcgcg
      121 ttctcggtct acccgatcgc ggcctcgctg ttttgcgagg cgcagatccc gaaacggctg
      181 atcccggcgg cgatcgggct gggcagcttc accttcacga tggttgcgct gccgggcacg
      241 ccggcgatcc agaacgcgat cccgattccg ttcttcaaga ccacgatcta cgccgcgccc
      301 ggcatgggcc tgatcgccgc ggtcttcatg ttcgccgtcg gcatgctctg gctgacgcgc
      361 cgctcgcacg cggcgcgcaa gagcggcgaa ggctacggcg agcacaagga ggaattcacc
      421 gcggtcgaca actcgaacct gccctcgttc ttcatggcgc tgctgccgat cctggcggtg
      481 atcgggctca acttcgcgct ctcgaaatac gtgatcccgg ccttcgacca cagctacctc
      541 gacaccgcga agttcaagca catcgagctg aagaaggtgc tcgcgatctg ggcgatcatc
      601 ggcgcaatgg cggcggcgat cgtcgtgctc gcgctgacga accgcgggcg gattgccgac
      661 ctgcgcgacg ccttcaacaa gggcacgctg ggctcgctgc tgccgatggc gaacacggcc
      721 tccgaggtcg gctatggcgc cgtgatcgcc tccctcgcga ctttcgcggt ggtgcgcgag
      781 ggcctgaagt cggtcgcctc cgaccccgtc gtctcgctgt ggctggtggt gacggtactg
      841 gccgcgatga cggggtcggc ctcggggggc atgagtctgg cgctcggtgc gatgggcgag
      901 tactacgccc atgccgccga agcggccaag atcaaccccg agatcatgca ccgcatcgca
      961 gcgatgggct ccgccgcgat ggacatcctg ccgcacaacg gcgcggtgat cacgctgctg
     1021 gcgatctgcg ggctcacgca caagcaatcg tacttcgacc tgttcgtggt cggcagcctg
     1081 accgcgttca gcgcgagcat cctggtgctg ggcctgtacc tgaccgtggg ggtgttctga
     1141 tgttgcgcag cgtgttgatg atgggcctgg cgctggccag cgccacagcg ccggcgcaaa
     1201 cgctgcgctt gctcgccgcc gagctgccgc cctacaccta ccaggtgccg tcggcctcgg
     1261 tcgccgagca acccgggccg ggccgcggcg tgatccacga actggtcgcc gaggccgcgc
     1321 gccgcgtcgg ccactcgggc cggatcgagt acttgccgtg gtaccgcgca caggagatcg
     1381 cgcagacgga aggcttcgtc ggcatcctcg cgctgacgcg ctcgcccgag cgcgaggaca
     1441 agtaccgctg gctgctgcac ctgatggacg acgacctggt gctggtcggc tcgcccggcg
     1501 tcgacgtgtc ggatttgtcg aaggtgcgcg accgcccggt cggcgtgctg cagcgctccg
     1561 gcgccgaggc gctgctgcgc tcgctcggct tcgcgcgcgt gtcgccgcag cccgaggagt
     1621 ggatgaacgc caagcgcatc aaggatcgca gcatcaacgc ctgggtcgcc ccgcgcgcga
     1681 tggtcgtgca cgcggtgcgc gaggtgcgcg gcaacctcga caccttgcag ttcggccagg
     1741 tcgtgcgcac ctccgaactc tggctcgcga cctcgaagag cctgcccgac gccgaggcga
     1801 agaagtggca ggacgcctgg gccgcgatgc gcgccgacgg cagcgccgag cgcatcgtcc
     1861 gccgcaacct gaacccacgc gtcgacccga tcgaagacgc gaagcgcctg atccaggaag
     1921 agcccttccg ctgaagctga aacgttcgag gccccgccat gcattcacgc cacaccgccc
     1981 tcgccgccct cacgcaaacc caacgcggca aggtcgtcag cgcgcacgac gccgtgcgcc
     2041 tgatccgcga cggcgacacg gtcgccaccg gcggcttcgt cggcatcggc ttcgccgaag
     2101 agatcgcgct cgcgctcgaa gcgctctacc tcgaaaccga caccgccgac gcccagtcga
     2161 ccggcaagcc gcgcaacctg acgctggtct acgccgccgg ccagggcgac ggcaagcaca
     2221 agggcctgaa ccacttcgcc cacgagggcc tggtcaagcg cgtgatcggc ggccactggg
     2281 ggctgtgccc gaagctgcag cagatggcgg cgggcaaccg catcgaggcc tacaacctgc
     2341 cgcagggcgt gatctcgcac ctgttccgcg atatcgccgg cggcaagccg gggcacttgt
     2401 cgagcgtcgg cctgggcacc ttcgtcgacc cgcgcttcgg cggcggcaag ctgaacgcgc
     2461 gcaccacgca agacatcgtg cgcctgatgc cgatcgacgg ccgcgactac ctcttctacc
     2521 aggccttccc gatccaggtc ggcatcatcc gcggcaccac cgccgacccc gacggcaaca
     2581 tcacgatgga acgcgaggcg ctgacgctcg aggcgctggc gatcgcgatg gcggtgcaca
     2641 actcgggcgg catcgtgatc gtgcaggtcg agcggctcgc ggcgcggcac gcgttgtcgg
     2701 cgcgcgaggt caaggtgccg ggcgcgctgg tcgactgcgt cgtcgtcgcc cggcccgaga
     2761 accactggca gaccttcgtc gaaccgtaca gcgcggcctt cagcggcgag ctgcgcgtgc
     2821 cgcaggacag cgccgggccc acggcgccga tgccgctgaa cgagcgcaag atcatcgccc
     2881 gccgcgccgc gctcgagctc gacgccaaca gcgtcgtcaa cctgggcatc ggcatgcccg
     2941 a
//
LOCUS       DASRDG010000022         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_236419, whole genome shotgun sequence.
ACCESSION   DASRDG010000022 DASRDG010000000
VERSION     DASRDG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236419"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFA35_00295"
     CDS             <1..642
                     /locus_tag="VFA35_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZT54633.1"
                     /translation="AEFAAEFDSSLVYDCDVANDDQIDAIFADLAKVWPEFDGFVHSI
                     GFAPKEAIAGDFLEGLSRESFRIAHDISAYSFPAMAKAAAAHLRPGSALLTLTYLGAL
                     RSMPNYNTMGLAKASLEASVRYLAASLGPKGVRVNAISAGPIKTLAASGIKGFGKMLE
                     ASSHSPLGRNVTIDDIGNVAAFLLSDLAAGITSEITYVDGGFSQATLGGLADA"
     gene            653..1612
                     /locus_tag="VFA35_00300"
     CDS             653..1612
                     /locus_tag="VFA35_00300"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HZT54634.1"
                     /translation="MTARLLGDVGGTNARFAWQDSDGAPLRDIATIPTADHPTLAAAL
                     ARYLRGIERAAPPWCAIGIANPITGDQVRMTNSHWSFSIEAVRRELGFERFAVINDFT
                     ALALALPDLPEKDLRQLGGGAAVAGTPLGLIGPGTGLGVSGLVPGIDPGRWVALQGEG
                     GHVTLAASNAREAAVLQVLRERFGHASAERAISGQGLEALHDAVCTLSGNPNAPPNAL
                     TAAEISRRALAASDPRCVEALDLFCAFLGNVAGNLALTLGARGGIYIGGGIVPRLGDA
                     FTNSRFRACFEDKGRFRSYLEPIPVYVIHSEVSPALLGAARAL"
     gene            complement(1620..>3141)
                     /locus_tag="VFA35_00305"
     CDS             complement(1620..>3141)
                     /locus_tag="VFA35_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZT54635.1"
                     /translation="HHAVAPGDVVVVDRNCHKSILHAIIMTGAVPVFLRPTRNHYGII
                     GPIPQSEFSPEAIRKKIAANPLLKGVDTKTVKPRILTLTQSTYDGVLYNTETVKHSLD
                     GYIDTLHFDEAWLPHAAFHRFYGQFHAMGKNRPRPKDQLVFSTQSTHKLLAGLSQASQ
                     VLVQDSQERKLDRSLFNEAYLMHSSTSPQYAIIASCDVAAAMMEAPGGPALVEESISE
                     SMDFRRAMRKVEKDYGKKDWWFKVWGPDRLAKEGIGKSEDWVLEAGAKWHGFGDLAEG
                     FNLLDPIKCTLITPGLDMSGKFADTGIPAAIVTKFLAEHGVIVEKTGLYSFFIMFTIG
                     ITKGRWNTLLTALQQFKDDYDRNQPLWRILPEFCAAHPRYERMGLKDLSQAIHKMYAK
                     GDIARLTTEMYLSDLQPAMKPADAFACIAHRKTERVPIDALEGRITTSLLTPYPPGIP
                     LLIPGERFNKKIVDYLKFTREFNSAFPGFDTDVHGLVESGDDAGDHLYFVDCVAAD"
BASE COUNT          460 a         1071 c         1073 g          537 t
ORIGIN      
        1 gccgagttcg ccgccgagtt cgattcgagc ctggtctacg actgcgacgt ggcgaacgac
       61 gaccagatcg acgcgatctt cgccgacctc gccaaggtct ggcccgagtt cgacggtttc
      121 gtgcactcga tcggcttcgc gccgaaagag gcgatcgccg gcgactttct cgagggcctg
      181 tcgcgcgaat cgttccgcat cgcgcacgac atctcggcgt acagcttccc ggcgatggcc
      241 aaggccgcgg ccgcgcacct gcgcccgggc agcgccttgc tcacgctgac ctatctcggc
      301 gcgctgcgca gcatgccgaa ctacaacacg atgggcctgg cgaaagcctc gctcgaagcc
      361 agcgtgcgct acctggccgc ctcgctcggc ccgaagggcg tgcgcgtcaa cgccatctcg
      421 gccgggccga tcaagacgct cgccgcctcg ggcatcaagg gcttcggcaa gatgctagaa
      481 gcctcgagcc actcgccgct gggccgcaac gtgacgatcg acgacatcgg caacgtcgcc
      541 gcgttcctgc tgtccgacct cgccgccggc atcacctccg aaatcaccta cgtcgacggc
      601 ggcttcagcc aggcgacgct gggcggcctg gccgacgcct gaagaggtcg acgtgacggc
      661 tcgactgttg ggtgacgtcg gcggcacgaa cgcccgcttc gcctggcaag acagcgacgg
      721 cgcgccgctg cgcgacatcg cgacgatccc gaccgccgac cacccgacgc tcgccgccgc
      781 gctggcgcgc tacctgcgcg gcatcgagcg cgcggcgccg ccgtggtgcg cgatcggcat
      841 cgcgaacccg atcaccggcg accaggtgcg gatgacgaac agccactggt cgttctcgat
      901 cgaggcggtg cggcgcgaac tcggcttcga gcgcttcgcg gtgatcaacg acttcaccgc
      961 gctggcgctg gcgctgcccg atctgccgga gaaagacttg cgccagctcg gcggcggcgc
     1021 ggccgtggcc ggcacgcccc tgggcctgat cggcccgggc accggcctgg gcgtgtccgg
     1081 gctggtgccg ggcatcgacc cggggcgatg ggtcgcgctg cagggcgaag gcggccacgt
     1141 gacgctcgcc gcgagcaatg cgcgcgaggc cgcggtgctg caggtgctgc gcgagcggtt
     1201 cggccacgcg tcggccgagc gcgcgatctc gggccagggc ctggaggcgc tgcacgacgc
     1261 cgtgtgcact ttgagcggca acccgaacgc cccgccgaac gcgctgactg cggccgagat
     1321 cagccgccgt gcgctggcag cgtctgaccc gcgctgcgtc gaagcgctcg acctgttctg
     1381 cgcctttctc ggcaacgttg ccggcaacct cgcgctgacg ctcggtgcgc gtggcggcat
     1441 ctacatcggc ggcggcatcg tgccgcgcct gggcgatgcg ttcacgaact cgcgctttcg
     1501 cgcctgcttc gaggacaagg gacgctttcg cagctacctc gagccgatcc cggtctacgt
     1561 gatccattcc gaggtatcgc ccgcgctgct cggcgcggcg cgcgccctct gaaacaaggc
     1621 taatcggccg cgacgcagtc gacgaagtag aggtgatccc cggcgtcgtc gccggattcg
     1681 accagcccgt gcacgtcggt gtcgaagccc gggaacgccg agttgaactc gcgcgtgaac
     1741 ttcaggtagt cgacgatttt cttgttgaag cgctcgcccg ggatcagcag cgggatgccc
     1801 ggcgggtacg gcgtcagcag cgacgtcgtg atgcggcctt cgagcgcgtc gatcggcacg
     1861 cgttcggtct tgcggtgcgc gatgcaggcg aacgcgtcgg ccggcttcat cgcgggctgc
     1921 aggtccgaca ggtacatctc ggtcgtcagc cgcgcgatgt cgcccttcgc gtacatcttg
     1981 tggatcgcct gcgacaggtc cttcagcccc atgcgttcgt agcgcgggtg cgcggcgcag
     2041 aactcgggca ggatgcgcca cagcggctgg ttgcggtcgt agtcgtcctt gaactgctgc
     2101 agcgcggtca gcagcgtgtt ccagcggccc ttggtgatgc cgatcgtgaa catgatgaag
     2161 aacgagtaca ggccggtctt ctcgacgatc acgccgtgct cggcgaggaa cttggtgacg
     2221 atcgccgccg gaatgccggt gtcggcgaac ttgcccgaca tgtccagccc cggcgtgatg
     2281 agcgtgcact tgatcgggtc gagcaggttg aagccttcgg ccaggtcgcc gaagccgtgc
     2341 cacttggcgc cggcctcgag cacccaatcc tcgctcttgc cgatgccctc tttcgcgagc
     2401 cggtcggggc cccagacctt gaaccaccag tccttcttgc cgtagtcctt ctcgaccttg
     2461 cgcatcgcgc ggcgaaaatc catcgactcc gagatgctct cttcgaccag cgccggcccg
     2521 cccggcgctt ccatcatcgc ggcggccacg tcgcagctcg cgatgatcgc gtactgcggg
     2581 ctggttgagc tgtgcatcag gtaggcctcg ttgaacaggc tgcggtcgag cttgcgctcc
     2641 tgcgagtcct gcacgagcac ctgcgaggcc tggctgaggc cggcgagcag cttgtgggtc
     2701 gactgcgtgg agaacacgag ctggtccttc ggccgcgggc ggttcttgcc catcgcgtgg
     2761 aactggccgt agaacctgtg gaacgcggcg tgcggcagcc aggcttcgtc gaagtgcaag
     2821 gtgtcgatgt agccgtcgag cgagtgcttg acggtttcgg tgttgtagag cacgccgtcg
     2881 taggtgctct gcgtcagcgt caggatgcgc ggcttcacgg tcttcgtgtc cacgcccttc
     2941 agcaacgggt tcgcggcgat cttcttccgg atcgcctcgg gcgagaactc gctctgcgga
     3001 atcggcccga tgatcccgta gtggttgcgc gtgggccgca ggaacaccgg caccgcgccc
     3061 gtcatgatga tcgcgtgcag gatcgacttg tggcagttgc ggtcgaccac gaccacgtcg
     3121 cccggcgcga ccgcgtggtg c
//
LOCUS       DASRDG010000023         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1157207, whole genome shotgun sequence.
ACCESSION   DASRDG010000023 DASRDG010000000
VERSION     DASRDG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157207"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..638)
                     /locus_tag="VFA35_00310"
     CDS             complement(132..638)
                     /locus_tag="VFA35_00310"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HZT54636.1"
                     /translation="MIDRPLTRRSFLPAGLALGGLGLRLANAQAADAPASAPGGLTLA
                     PASMSGVSPRLTVHAIDSFHGVTGAGLKVDLARYEGDRWVPLKSVQALAGGRPAEPLL
                     LGDAYKAGRYEVLLHLDDYFATMGAKLPQPPFLAKVPLRFVIADAGQRVHLAALFSPW
                     GYSYYRGS"
     gene            complement(665..1174)
                     /gene="uraD"
                     /locus_tag="VFA35_00315"
     CDS             complement(665..1174)
                     /gene="uraD"
                     /locus_tag="VFA35_00315"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HZT54637.1"
                     /translation="MTLAQLAALDRAAYMTAFGGLFEHSPWVAEAAWAQRPFASEAAL
                     HDAMMNAVRDAPQPQQIAFLNLHPELAGKEAQAGTMTDHSTFEQHGAGLNALGRDELL
                     ELQRLNAAYTERHGFPFIIAVLGHTKPQIFEALRTRTGRDTDTERQEALAQIAQITRR
                     RLNALFAVS"
     gene            complement(1171..2460)
                     /locus_tag="VFA35_00320"
     CDS             complement(1171..2460)
                     /locus_tag="VFA35_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT54638.1"
                     /translation="MPSALDATYSRITWRLIPFLFVLWVLAWVDRVNVGFVKLTMLDE
                     LKWSETVYGLGAGIFFLGYFFFEVPSNLLLQRIGARKTITRITFGWGVVCVAMMFVTT
                     PGMFYFLRFLMGVFEAGFYPGIILYLTYWYPSQRRAKAFGMFMSASGIAGVIGGPLAG
                     TVMNGLHGMNGWSGWQWVFLIEGIPSIVAAVVTWFYLTDRPAQAGWLTPAQRQLVEDD
                     LARDAASFGKREESLLASLKEGRLWLLILVFFCVIAANSSLTFFGPSVVKEVGFSNPA
                     TVGWIMSLSYLCGAIGMVVNGLHSDRANEARLHCAIAAALGAAGLLLTALFIKTNPTL
                     ALAAQTLAIIGTMSAIPVFWQLPGRFLAGAAAAGGVALINSIANLAGFGAPFMLGVIK
                     TQTGGLAPGLLIVAAVEVCATVLILLFIPRFAKKATP"
BASE COUNT          419 a          839 c          894 g          342 t
ORIGIN      
        1 tcagcttcca gctgccgtcg gcctgcctgg ccgaaaagtc gatgcgcatg ccggcgccgg
       61 ggcggccggt gtagttgtcg agaacgtggg tcgagatgcc tgccatggtg tgtgctcgga
      121 ggtgagtggg ttcaggagcc gcggtagtac gagtagcccc acggcgagaa cagcgccgcg
      181 aggtgcacgc gctggcccgc gtcggcgatc acgaagcgca gcggcacctt ggccaggaac
      241 ggcggctgcg gcagcttcgc gcccatcgtc gcgaagtagt cgtcgaggtg cagcagaacc
      301 tcgtagcggc cggccttgta cgcatcgccg agcagcagcg gctcggccgg acggccgccg
      361 gccagcgcct gcacgctctt cagaggcacc cacctgtcgc cttcgtagcg cgccaggtcg
      421 accttgaggc cggcgccggt gacgccgtgg aacgagtcga tcgcgtgcac ggtcaggcgc
      481 ggcgagacgc cggacatcga cgccggcgcc agcgtcaaac cgccgggcgc ggaggccggc
      541 gcatcggccg cctgcgcgtt cgccagccgc aggccgaggc caccgagcgc gaggccggca
      601 ggcaggaagg atcggcgggt caggggtcgg tcgatcatgg ggttctctcg tcagtgggtg
      661 aggatcagga aacggcgaac agcgcattca ggcggcggcg cgtgatctgc gcgatctgcg
      721 ccagcgcttc ctggcgctcg gtgtcggtgt cgcgcccggt gcgcgtgcgc agcgcctcga
      781 agatctgcgg cttggtgtgg cccagtaccg cgatgatgaa agggaagccg tggcgctcgg
      841 tgtaggcggc gttcaggcgc tgcagctcca gcagttcgtc gcggcccagc gcgttcaggc
      901 cggcgccgtg ctgctcgaag gtcgagtggt cggtcatcgt gccggcctgc gcctccttgc
      961 cggccagctc ggggtgcagg ttgaggaacg cgatctgctg cggctgtggt gcatcgcgca
     1021 ccgcgttcat catcgcgtcg tgcagcgccg cctcgctcgc gaacgggcgc tgcgcccacg
     1081 cggcctcggc cacccacggc gaatgctcga acagcccgcc gaacgccgtc atgtaggcgg
     1141 cgcggtccag tgccgccagt tgcgcgagcg tcatggcgtc gccttcttcg cgaagcgcgg
     1201 gatgaacagc aggatcagca cggtcgcgca gacctcgacc gccgcgacga tcagcagccc
     1261 cggcgcgagc ccgccggtct gcgtcttgat cacgccgagc atgaacggcg cgccgaagcc
     1321 ggcgaggttc gcgatcgagt tgatcagcgc gaccccgccg gccgccgccg caccggcgag
     1381 gaagcgcccg ggcagctgcc agaacaccgg aatggcgctc atcgtgccga tgatcgcgag
     1441 cgtctgcgcg gccagcgcga gcgtcgggtt agtcttgatg aacagcgcgg tcagcagcag
     1501 gccggccgcg ccgagcgccg cggcgatcgc gcagtgcagc cgcgcctcgt tcgcgcggtc
     1561 ggagtgcagg ccgttgacga ccatgccgat cgcaccgcac aggtacgaca gcgacatgat
     1621 ccagccgacc gtcgccgggt tcgagaagcc gacctccttg accaccgacg ggccgaagaa
     1681 ggtcagcgac gagttcgccg cgatcacgca gaagaacacc aggatcagca gccacagccg
     1741 cccttccttc agcgacgcga gcaggctttc ctctcgcttg ccgaacgaag cggcgtcgcg
     1801 cgcgaggtcg tcctcgacca gctggcgctg cgccggcgtg agccagcccg cctgcgcggg
     1861 ccggtcggtc aggtagaacc aggtcacgac cgccgcgacg atcgacggga tgccttcgat
     1921 caggaacacc cactgccagc ccgaccagcc gttcatgccg tgcaggccgt tcatcacggt
     1981 gccggcgagc ggcccgccga tcacgccggc gatgcccgac gccgacatga acatgccgaa
     2041 ggccttggcg cgccgctggc tcgggtacca ataggtcagg tacaggatga tgccggggta
     2101 gaagccggcc tcgaacacgc ccatcaggaa gcgcaggaaa tagaacatcc ccggcgtcgt
     2161 cacgaacatc atcgcgacgc agacgacacc ccagccgaag gtgatgcgcg tgatcgtctt
     2221 gcgcgcgccg atgcgctgca acagcaggtt cgacggcacc tcgaagaaga agtagccgag
     2281 gaagaagatg ccggcgccca ggccgtagac ggtctcgctc cacttcagtt cgtcgagcat
     2341 ggtcagcttg acgaagccga cgttgacgcg gtcgacccag gccagcaccc acagcacgaa
     2401 caggaacggg atcaggcgcc aagtgatgcg gctgtaggtc gcgtcgagtg cgctgggcag
     2461 cggcgcggcg aggcccgagg gggaggcggc ttcg
//
LOCUS       DASRDG010000024         6511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_465034, whole genome shotgun sequence.
ACCESSION   DASRDG010000024 DASRDG010000000
VERSION     DASRDG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465034"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFA35_00325"
     CDS             complement(<1..755)
                     /locus_tag="VFA35_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT54639.1"
                     /translation="MNWHAIKAIYGVEMARTWRTIMQSIISPVLSTALYFVVFGAAIG
                     SRIAHVDGVSYGAFIVPGLIMLSVLTQSISNASFGIYFPKFTGTIYELLSAPISNVEI
                     VLSYVGAAATKSVAIGLIILATAALFVPIRIEHPVWMLLFLVLTAVTFSLFGFIIGIW
                     ADGFEKLQMIPMLIVMPLTFLGGPFYSIKMLPPVWQQISMLNPSVYLISGFRWSFFGQ
                     SELHVGTSFSVTLVFFFVALGVATWILKTGWRI"
     gene            complement(752..1678)
                     /locus_tag="VFA35_00330"
     CDS             complement(752..1678)
                     /locus_tag="VFA35_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT54640.1"
                     /translation="MASIISVADVSKTYATGFRALDHIDLEIEQGEIFALLGPNGAGK
                     TTLIGLICGIVRLDQGRVSVAGLDIRADYRAARALIGLVPQELTTDAFETVWATVSFS
                     RGLFGKAPDPAYIEKTLRALSLWDKKDNKIITLSGGMKRRLMIAKALSHEPRVLFLDE
                     PTAGVDVALRKDMWAMVRRLRESGVTIVLTTHYIEEAEDMADRVGVISHGRLILVEDK
                     TELMKKLGKKRLTVQLRAPLAQLPASLAAHALVLSADGSELAYAYDPRASQGAVAALL
                     DDLSAAGIGLKDLHTTQSSLEDIFVSLVEEKA"
     gene            1806..2861
                     /locus_tag="VFA35_00335"
     CDS             1806..2861
                     /locus_tag="VFA35_00335"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZT54641.1"
                     /translation="MSLAQAYAAQAATTPLAPFTIERREPGAGDVAIAILHCGVCHSD
                     LHAARGDWGNTVYPCVPGHEIVGRVTAVGTLVSKFKVGDLVGVGCMVDSCQHCRPCFD
                     GLEQFCDKGYTDTYNGKEQASRANTLGGYSTHIVVREKFVLRVTHAESQLAAVAPLLC
                     AGITTWSPLRQWKVGPGQKVGIVGLGGLGHMGVKLAAALGAHVVLFTTSPGKTEDALR
                     LGAKEVVVSKNRDEMKAQRNQLDFILNTVAVPHNLDHFLALLKLDGTMTLVGAPASPH
                     PAPSVISLIAKRRRLAGSLIGGIAETQEMLDFCAERGIVADIEPIAMAQIETAYERMH
                     KSDVKYRFVIDMATMNA"
     gene            2884..4461
                     /locus_tag="VFA35_00340"
     CDS             2884..4461
                     /locus_tag="VFA35_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HZT54642.1"
                     /translation="MTPSKRAAWLLALGALVLGLHGCAVLPPPAEREVSVAVPASANE
                     PLGRIALQSVPAGQDSGFRPLPLANWSMDARLTLVREARKTLDLQFYLLQNDVTGHTL
                     ARAVRDAALRGVRVRLLVDDLYTAENIHLLLGLAAYPNVEVRLFNPFAGGRGFGLTRW
                     LVGLNEFSRINHRMHNKLMVADGAFAIAGGRNMADEYFFSSKGGNFVDFDLLVAGAAV
                     PRLAAIFDTYWNSPRIYPLDAIETARHDARSQQEAFEALTLDAIGAHPSPAPGRSDIL
                     GFRTLSDDLQNPPLKLLHGRVDVFADDPEKVSGRAERGGDDTTVTARVGKAMSEAKTE
                     VLVGSPYFIPGQLGMDGLRAARSHGVKVEVMTNSLASNDEPFASAAYSNYRVPMLKLG
                     AQLYETDSSQLKNDKLIGGALQNTVGRSHSKLIVIDRRTTFVGSMNMDFRSSRVNTEL
                     GMLVDSPELAEMVIALAARVREGSYRLRLAEGDGHLQWVGIENGAEKVWDSEPGVDFA
                     TRLQLLLLFPFISESLL"
     gene            complement(4471..5262)
                     /locus_tag="VFA35_00345"
     CDS             complement(4471..5262)
                     /locus_tag="VFA35_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZT54643.1"
                     /translation="MTVGAELWLSLDGRNLAGARRVALLAELARVGSITQAAKAAGFS
                     YKGAWNAIDDMSNQAGEPLLERKPGGKGGGSTRLTPRGAQLLRNFELIRREHARFVER
                     LNQQAHGLVEDYSLMESIAMKTSARNQFAGTVCSVRAGAINEEVELEVIGGVRIVAIV
                     TRESRHELGLEVGAKAFALVKASSIMLMTDAGDVRLSARNRLAGTVSRVVPGAVNTEV
                     VLQLAGGGSVAAVITNESATALGLAAGAPATAVFKASSVILGVAA"
     gene            complement(5285..6001)
                     /locus_tag="VFA35_00350"
     CDS             complement(5285..6001)
                     /locus_tag="VFA35_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010089113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT54644.1"
                     /translation="MSFDVDIDKTVRSDQRSFRLRVRFRAGARRSVIFGPSGAGKSLT
                     LQALAGLLTPDAGRIAFDGETLFDKAAGIDVPARARGFGYLFQDYALFPRLNVRQNIA
                     FGLAPGLRNPPANVGGAAVERWLQAFGLDEVAAQRPAELSGGQRQRTALARALVHSPR
                     ALLLDEPFSALDPELRDRMRAELDELLQRIDIPVVMITHDPEDLAWFGDTALYLRDGT
                     ITAAPAAAPERRMSTRIRAA"
     gene            complement(6006..>6511)
                     /gene="modB"
                     /locus_tag="VFA35_00355"
     CDS             complement(6006..>6511)
                     /gene="modB"
                     /locus_tag="VFA35_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HZT54645.1"
                     /translation="TVLGYYLLVLIGKRGWLGGWLQSSFGINLIFTWQGAVIAATIVA
                     FPLILKSARTAFEAVDPQFEQAARGLGLGEWAVFFRVTLPMAWPGILAGVLLVFARSL
                     GEFGATLMVAGSIPGRTQTLSIAVYEAVQAGQDDTANFLVGLTSIVCIVVLLAAGRLA
                     PRPHARS"
BASE COUNT         1063 a         2273 c         2229 g          946 t
ORIGIN      
        1 ttgatgcgcc agccggtctt cagaatccag gtcgcgacgc ccagcgcgac gaagaagaac
       61 accagcgtca ccgagaaact ggtgccgacg tggagttccg actggccgaa gaagctccag
      121 cggaacccgc tgatcaggta gaccgacggg ttcagcatcg agatctgctg ccacaccggc
      181 ggcagcatct tgatcgagta gaacgggccg ccgagaaagg tcagcggcat cacgatcagc
      241 atcgggatca tctgcagctt ctcgaagccg tcggcccaga tgccgatgat gaagccgaac
      301 aggctgaacg tgaccgccgt cagcaccagg aacagcagca tccagaccgg gtgctcgatg
      361 cggatcggca cgaacagcgc tgcggtcgcg aggatgatca gcccgatcgc gaccgacttg
      421 gtcgccgcgg cgccgacgta gctcagcacg atctcgacgt tcgagatcgg cgccgagagc
      481 agctcgtaga tcgtgccggt gaacttcggg aagtagatgc cgaacgatgc gttcgagatg
      541 ctctgcgtca gcaccgacag catgatcagg ccgggcacga tgaacgcgcc gtagctcacg
      601 ccgtcgacat gcgcgatgcg cgagccgatc gcggcgccga acacgacgaa gtacagcgcg
      661 gtcgacagca ccggcgagat gatgctctgc atgatcgtgc gccaggtgcg cgccatctcg
      721 acgccataga tcgccttgat cgcatgccag ttcatgcctt ctcctcgacc aggctgacga
      781 agatgtcctc gagcgaactc tgcgtcgtgt gcaggtcttt cagcccgatg ccggccgcgc
      841 tcaggtcgtc gagcagcgcg gcgacggcgc cctgcgaggc gcgcgggtcg taggcgtagg
      901 cgagctcgct gccatcggcg ctgagcacca gcgcgtgcgc ggccaggctc gcgggcagct
      961 gcgcgagcgg cgcgcgcagt tgcaccgtca gccgcttctt gccgagcttc ttcatcagct
     1021 cggtcttgtc ttcgaccagg atgagccggc cgtggctgat cacgccgacg cggtcggcca
     1081 tgtcctcggc ctcctcgatg tagtgcgtgg tcagcacgat ggtcacaccc gattcgcgta
     1141 agcgccgcac catcgcccac atgtccttgc gcagcgccac gtcgacgccg gcggtcggct
     1201 cgtcgaggaa cagcacgcgc ggctcgtgcg acagcgcctt ggcgatcatc aggcgccgct
     1261 tcatgccgcc ggagagcgtg atgatcttgt tgtccttctt gtcccacagc gagagcgcgc
     1321 gcagcgtctt ctcgatgtag gcggggtcgg gcgccttgcc gaacaggccg cggctgaacg
     1381 agacggtcgc ccagacggtc tcgaacgcat cggtcgtcag ctcctgcggc accaggccga
     1441 tcagcgcgcg cgccgcgcgg tagtcggcgc ggatgtcgag cccggcgacg ctgacccggc
     1501 cctggtccag ccggacgatg ccgcagatca acccgatcag cgtggtcttg ccggcgccgt
     1561 tcgggccgag cagcgcgaag atctcgccct gttcgatctc gaggtcgatg tggtcgagcg
     1621 cgcgaaagcc ggtggcgtag gtcttcgaga cgtcggcaac ggaaatgatg gaggccatgc
     1681 ggggcgggtg tttcggcggc gaagcatcga ccttacccca gccgcgcctt gaactcgccc
     1741 tcggccatca agagtggcaa gattgcgcag gcccgccagt cggccgaaca acctccggag
     1801 acaccatgag cctcgcccaa gcctacgcgg cacaagccgc gacgacaccg ctcgccccct
     1861 tcaccatcga gcggcgcgag ccgggcgcgg gcgacgtggc gatcgccatc ctgcactgcg
     1921 gcgtctgcca ctccgatctg cacgccgcgc gcggcgactg gggcaacacg gtctacccgt
     1981 gcgtgccggg gcacgagatc gtcggccgcg tcaccgcggt cggcacgctc gtgtcgaaat
     2041 tcaaggtcgg cgacctcgtc ggcgtcggct gcatggtcga cagctgccag cactgccggc
     2101 cctgcttcga cggactcgag cagttctgcg acaagggcta caccgacacc tacaacggca
     2161 aggagcaggc ttcgcgcgcg aacacgctgg gcggctactc gacccacatc gtcgtgcgcg
     2221 agaagttcgt gctgcgggtc acgcacgccg agagccagct cgccgcggtc gcaccgctgc
     2281 tgtgcgcggg catcacgacc tggtcgccgc tgcggcagtg gaaggtcggg ccagggcaga
     2341 aggtcggcat cgtcggcctg ggcgggctcg gccacatggg cgtgaagctc gcggcggcgc
     2401 tcggcgcgca cgtggtgctg ttcacgacct cgccgggcaa gaccgaggac gcgctgcgtc
     2461 tgggcgcgaa agaggtcgtg gtctcgaaga accgcgacga gatgaaggcg cagcgcaacc
     2521 agctcgattt cattctgaac accgtcgcgg tgccgcacaa cctcgaccat ttcctcgcgc
     2581 tgctcaagct cgacggcacg atgacgctgg tcggcgcgcc cgcgtcgccg cacccggcgc
     2641 cgagcgtgat cagcctgatc gccaagcgcc ggcgcctggc gggctcgttg atcggcggca
     2701 tcgccgagac gcaggagatg ctcgatttct gcgccgaacg cggcatcgtc gccgacatcg
     2761 agccgatcgc gatggcgcag atcgagaccg cctacgaacg catgcacaag agcgacgtga
     2821 aataccgctt cgtgatcgac atggcgacga tgaacgccta gcaaaccacc ggagagcttg
     2881 ccgatgacgc cctcgaaacg agccgcctgg ctgctcgcgc taggcgcgct cgtgctgggc
     2941 ctgcacggct gcgcggtgct gccgccgccg gccgagcgcg aagtcagcgt ggccgtgccc
     3001 gcgagcgcga acgagccgct cggccgcatc gcgctgcaaa gcgtgccggc cgggcaggac
     3061 tcgggctttc gtccgctgcc gctggccaac tggtcgatgg atgcgcggct gaccctcgtg
     3121 cgcgaagcgc gcaagacgct cgacctgcag ttctacctgc tgcagaacga cgtgaccggc
     3181 cacacgctcg cgcgcgcggt gcgcgatgcg gcgctgcgcg gcgtgcgcgt gcggctgctg
     3241 gtcgacgacc tgtacaccgc cgagaacatc cacctgctgc tcgggctcgc ggcctacccg
     3301 aacgtcgagg tgcggctgtt caacccgttc gccggcgggc gcggcttcgg cctgacgcgc
     3361 tggctcgtcg ggctgaacga gttctcgcgc atcaaccacc gcatgcacaa caagctgatg
     3421 gtggccgacg gcgcgttcgc gatcgccggt ggccgcaaca tggccgacga atacttcttc
     3481 agcagcaagg gcggcaactt cgtcgacttc gacctgctgg tcgcgggcgc cgccgtgccg
     3541 aggctcgcgg cgatcttcga cacctactgg aacagcccgc gcatctaccc gctggatgcg
     3601 atcgagaccg cgcggcacga cgcgcgatcg cagcaggaag cgttcgaggc gctcaccctc
     3661 gatgcgatcg gcgcgcaccc gtcgccggcg cccgggcgca gcgacatcct cggctttcgc
     3721 acgctgagcg acgacctgca gaacccgccg ttgaagctgc tccatggccg cgtcgacgtg
     3781 ttcgccgacg atcccgagaa ggtcagcggc cgcgccgaac gcggcggtga cgacacgacc
     3841 gtgaccgcgc gcgtcggcaa ggcgatgagc gaggccaaga ccgaggtgct ggtcggctca
     3901 ccgtacttca tccccgggca gctcggcatg gacggcctgc gcgccgcgcg ctcgcacggc
     3961 gtgaaggtcg aggtcatgac caactcgctc gcgtcgaacg acgagccgtt cgccagcgcc
     4021 gcctacagca actaccgcgt gccgatgctg aagctgggcg cgcagctgta cgagaccgac
     4081 tcgagtcagc tgaagaacga caagctgatc ggcggcgcgt tgcagaacac ggtcggccgc
     4141 tcgcactcga agctgatcgt gatcgaccgg cgcaccacct tcgtcggctc gatgaacatg
     4201 gacttccgct cgtcgcgcgt caacaccgag ctcggcatgc tggtcgattc acccgagctc
     4261 gccgagatgg tgatcgcgct cgccgcacgc gtgcgcgaag gcagctaccg gctgcggctg
     4321 gccgagggcg acggccacct gcaatgggtc ggcatcgaga acggcgccga gaaggtctgg
     4381 gacagcgagc ccggcgtcga cttcgccacg aggctgcaac tcctgctgct gttcccgttc
     4441 atcagcgaga gcctgctcta gaaccgaggc tcaagcggca acacccagga tcacgctcga
     4501 cgccttgaac accgcggtgg ccggggcgcc ggccgccagc ccgagcgcag tcgcgctctc
     4561 gttcgtgatc accgcggcca cgctgccgcc gccggccagc tgcagcacga cctcggtgtt
     4621 gaccgcgccg ggcacgactc ggctcaccgt gcccgcaagc cggttgcgcg ccgacaggcg
     4681 cacgtcgccg gcatcggtca tcagcatgat cgacgaggcc ttgaccagcg cgaacgcctt
     4741 cgcgccgacc tcgagcccga gttcgtggcg gctttcgcgc gtgacgatcg cgacgatgcg
     4801 cacgccgccg atcacctcga gctcgacctc ctcgttgatg gcgccggcgc gcacgctgca
     4861 caccgtgccg gcgaactgat tcctggcact ggtcttcatg gcgatactct ccatcagtga
     4921 gtagtcttcg accaggccgt gcgcctgctg gttcaaccgt tcgacgaagc gcgcgtgctc
     4981 gcgccggatc agctcgaagt tgcgcagcag ctgcgcgccg cgcggcgtga gccgggtcga
     5041 gccaccgccc ttgccgccgg gctttcgttc gagcagcggc tcgccggcct ggttgctcat
     5101 gtcgtcgatc gcgttccagg cgcccttgta gctgaagccc gccgccttcg cggcctgcgt
     5161 gatcgagccg acgcgcgcga gttcggccag cagcgcgacg cgccgcgcgc ccgcgaggtt
     5221 gcggccgtcg aggctgagcc agagctcggc gccgacggtc atcgggcgtg ggtcggccgt
     5281 cacgtcaggc cgcccggatg cgcgtcgaca tgcggcgctc gggcgccgcc gcaggcgctg
     5341 cggtgatcgt gccgtcgcgc aggtacagcg ccgtgtcgcc gaaccaggcg aggtcttcgg
     5401 ggtcgtgcgt gatcatcacg accgggatgt cgatgcgctg cagcagttcg tcgagttcgg
     5461 cgcgcatgcg gtcgcgcagt tcggggtcga gcgccgagaa cggctcgtcg agcagcagcg
     5521 cgcgcggcga gtgcacgagc gcgcgcgcga gcgcggtgcg ctggcgctgc ccgccggaga
     5581 gctcggccgg gcgctgggcg gcgacctcgt cgagcccgaa ggcctgcagc cagcgctcga
     5641 ccgcggcgcc gccgacgttc gccggcgggt tgcgcagccc cggggccagc ccgaacgcga
     5701 tgttctggcg cacgttgagg cgcgggaaca gcgcgtagtc ctggaacagg tagccgaagc
     5761 cgcgcgcgcg cgccggcacg tcgatgcccg cggccttgtc gaacagcgtc tcgccgtcga
     5821 acgcgatgcg gccggcgtcg ggcgtcagca gcccggcgag cgcctgcagc gtcaggctct
     5881 tgcccgcgcc cgaggggccg aagatcacgc tgcgccgcgc gccggcgcga aagcgcacgc
     5941 gcaggcgaaa gctgcgttga tcgctgcgca ccgtcttgtc gatgtcgacg tcgaaactca
     6001 ctcggtcagc tccgcgcgtg cggccgcggc gcgagccgcc cggccgcgag cagcacgacg
     6061 atgcagacga tcgaggtgag cccaaccagg aagttcgcgg tgtcgtcctg ccccgcctgc
     6121 accgcctcgt agaccgcgat cgacagcgtc tgggtgcggc ccggaatgct gccggccacc
     6181 atcagcgtcg cgccgaactc gccgagcgaa cgcgcgaaca ccagcagcac gccggcgagg
     6241 atgcccggcc aggccatcgg cagcgtcacg cggaagaaca ccgcccactc gcccaggccg
     6301 agcccgcgcg cggcctgctc gaactgcggg tcgaccgcct cgaacgcggt gcgcgccgac
     6361 ttcaggatca acgggaacgc gacgatcgtc gcggcgatca ccgcgccctg ccaagtgaag
     6421 atcaggttga tgccgaagct gctctgcagc cagccgccga gccagccgcg cttgccgatc
     6481 aggaccagca ggtagtagcc cagcacggtc g
//
LOCUS       DASRDG010000025         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1505832, whole genome shotgun sequence.
ACCESSION   DASRDG010000025 DASRDG010000000
VERSION     DASRDG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505832"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VFA35_00360"
     CDS             <1..1015
                     /locus_tag="VFA35_00360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZT54646.1"
                     /translation="YLFEYVAFTLVCALVLLRRARRYDVVHVHNMPDLLVFAAVAPRL
                     CGRAVVLDVHDLMPEVWASKFKTSPRHPAILPLRAQEWLSHRFASAVVFATERFRAVA
                     LGRGTVAAARSVAVMNAADTRVFDRRRHPWAGPGEASAFHMLYLGTVSHRHGVDQCVR
                     VLPLLAREIPGIRLIVHSKLCADEGQPLAELRALAHKLGVADLLELRPSLPLHEVPAV
                     MSRASVGVFTPHLDVHIDMALSLKVPEFVAMGVPVVAVRTSIMESLFSSDEVLLFEDG
                     DLEAFADRLRRLHQDPQLGRQLAARADRFTKAHAWEAEFARYLDLLGRLTGRAPAPAV
                     LQS"
     gene            1017..3404
                     /locus_tag="VFA35_00365"
     CDS             1017..3404
                     /locus_tag="VFA35_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZT54647.1"
                     /translation="MLRRLCLLAATPLLLPVAAVAVAAVAWPLLLLVLLVDLLWWPVA
                     LLRRRPLPYLPRDARSASIVTVTWNGRHWLEVLLPSLRAVVQEHGGDHEVIVVDNGST
                     DGSVEWLHREHPWVKVVALPENRFFVRGNLAGVQAATRDVLVFLNNDMEVKPGFLQPL
                     LDGLRDPKVFAVAAEVFFRNPHKRREETGRTRGAVARGWLKLAHVEPTRDERALDLVP
                     TFWAGGGSAAFDRRVYLAIGGFDTLYDPFYMEDTGLSYQAWRRGFRVMFTARAAVLHE
                     HRGTSRKVFGDDYIDNVIRRNQHLFLWRNVTDPGLTAAVLGGLPLSVFARARRPRGSY
                     LAGLWFELRAFLRALPRFPEALRKRCASRGSYRLTDRQVAAAANGIAAHRALCGTGLG
                     ALPVPDARRGRRILVLSARLPRRDHDGSWVLFERLRAMAARHRVTLFAFVDDPAEAIA
                     TAPLSEAGIEVVIEPRQRNRRPGNLHHRVPWRLWRDYSAPTMRAAVQRMLEGTDHDLV
                     QVEYLEMAHLVAPEPRVRPRLYVCHESAAVAAARTARGPGGAFRALQADRYERRLLRA
                     FDGAVALSAADAAALAVQAPSLPVEIVPSGIDVAAFAAGSGNGAVSAADPATIAFVGY
                     YKHAPNVDAAQWLVHEILPRVRQAVPAARVRLIGRDAPAEVAALAREGAVEVPGFVPD
                     LAGALAHATVVALPLRQGGGLRGKLLEAWAAGRPVVTTAVACEGYEVAAGEHCLVADD
                     APAFAAALVRCLQEPLLRDSLGRAGRAHCTEHYSVAAAAATFDGVYERLCAGRTQ"
     gene            3401..>3585
                     /locus_tag="VFA35_00370"
     CDS             3401..>3585
                     /locus_tag="VFA35_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54648.1"
                     /translation="MKIALFLTTELGPARILLDKVLDRHGGEVPSVFVRDEHRVPLTA
                     DLEHCDVRRDKPAGGKLA"
BASE COUNT          410 a         1327 c         1334 g          514 t
ORIGIN      
        1 ctacctgttc gagtacgtcg cgttcacgct ggtgtgcgcg ctcgtgctgc tgcggcgggc
       61 gcgccgctac gacgtcgtgc acgtgcacaa catgcccgac ctcctcgtgt tcgcggccgt
      121 cgcgccgcgg ctgtgcgggc gcgccgtcgt gctcgacgtg cacgacctga tgcccgaggt
      181 gtgggcgagc aagttcaaga cctcgccgcg ccatcccgcg atcctgccgc tgcgcgcgca
      241 ggagtggctg tcgcaccggt tcgccagcgc ggtggtgttc gcgaccgaac ggttccgcgc
      301 cgtcgcgctc ggccgcggca ccgtcgccgc ggcgcgcagc gtcgcggtga tgaacgccgc
      361 cgacacgcgc gtgttcgacc gccggcggca cccgtgggcc ggacccggcg aggcctctgc
      421 gttccacatg ctctacctcg gcaccgtgtc gcaccggcac ggcgtcgacc agtgcgtgcg
      481 cgtgctgccg ctgctcgcgc gcgagatccc gggcatccgg ctcatcgtgc acagcaagct
      541 ctgcgccgac gaagggcagc cgctcgcgga gctgcgcgcg ctcgcgcaca aactcggcgt
      601 cgccgacctg ctcgaactgc ggccgtcgct gccgctgcac gaagtgcccg ccgtgatgag
      661 ccgcgcgtcg gtcggcgtgt tcacgccgca cctcgacgtg cacatcgaca tggcgctgtc
      721 gctcaaggtg cccgagttcg tcgcgatggg cgtgccggtg gtcgcggtgc gcacgtcgat
      781 catggagtcg ctgttctctt ccgacgaggt gctgctgttc gaggacggcg acctcgaggc
      841 gttcgcggac cggctgcggc gcctgcacca ggacccgcag ctcggccggc agctggccgc
      901 gcgcgccgac cgcttcacga aagcgcacgc ctgggaggcg gagttcgcgc gctatctcga
      961 tctgctcggg cgcctcactg gccgcgcgcc ggccccggcg gtactacaga gctgagatgc
     1021 tgcgccggct gtgcctcctc gccgcgacgc cgctgctgct gccggtcgcg gcggtggcgg
     1081 tcgcggcggt cgcgtggccg ttgctgctgc tggtgctgct cgtcgacctg ctgtggtggc
     1141 cggtcgctct gctgcgccgg cggccgctgc cgtacctgcc gcgcgacgcg cgctcggcgt
     1201 cgatcgtcac cgtgacctgg aacggcaggc actggctcga ggtgctgctg ccgtcgctgc
     1261 gcgccgtggt gcaggagcac ggcggcgacc acgaggtgat cgtcgtcgac aacggcagca
     1321 ccgacggttc cgtcgagtgg ctgcaccgcg agcacccctg ggtcaaggtc gtcgcgctgc
     1381 ccgagaaccg cttcttcgtg cgcggcaacc tcgccggcgt gcaggcggcg acgcgcgacg
     1441 tgctggtgtt cctcaacaac gacatggagg tcaagcccgg cttcctgcag ccgctgctcg
     1501 acgggttgcg cgacccgaag gtgttcgcgg tcgccgcgga ggtgttcttc cgcaacccgc
     1561 acaagcgccg cgaggagacg ggtcgcacgc gcggcgcggt cgcgcgcggc tggctcaagc
     1621 tcgcgcacgt cgagccgacg cgcgacgagc gcgcgctcga cctggtgccg acgttctggg
     1681 ccggcggcgg cagcgccgcg ttcgaccggc gcgtctacct cgcgatcggc ggcttcgaca
     1741 cgctctacga cccgttctac atggaggaca cggggctgtc gtaccaggcg tggcggcgcg
     1801 gcttccgcgt catgttcacg gcccgcgccg cggtgctgca cgaacaccgc ggcaccagcc
     1861 gcaaggtgtt cggcgacgac tacatcgaca acgtcatccg ccgcaatcag cacctgttcc
     1921 tgtggcgcaa cgtcaccgac ccgggcctca ccgcggcggt gctcggcggg ctgccgctgt
     1981 cggtgttcgc gcgcgcgcgg cggccgcgcg gcagctacct cgcggggctg tggttcgagc
     2041 tgcgcgcgtt cctgcgcgcg ctgccgcggt tccccgaggc gctgcgcaaa cgctgcgcgt
     2101 cgcgcggcag ctaccggctc accgaccggc aggtcgcggc cgccgcgaac gggatcgccg
     2161 cgcaccgcgc gctctgcggc accgggctcg gcgcgctgcc ggtgcccgac gcgcggcgcg
     2221 gccgcaggat cctcgtgctc agcgcgcggc tgccgcgccg cgaccacgac ggctcgtggg
     2281 tgctgttcga acggctgcgc gcgatggcgg cgcggcaccg cgtcacgctg ttcgcgttcg
     2341 tcgacgatcc tgccgaggcg atcgccaccg cgccgctcag cgaggccggc atcgaggtcg
     2401 tgatcgagcc gcgccagcgc aaccggcggc ccggcaacct gcaccaccgc gtgccgtggc
     2461 ggctgtggcg cgactactcg gcgccgacca tgcgcgccgc cgtgcagcgc atgctcgaag
     2521 gcaccgacca cgacctcgtc caggtggagt acctggagat ggcgcacctg gtcgcgccgg
     2581 agccgcgcgt ccggccgcgg ctctacgtct gccacgagtc cgcggcggtg gcggcggcgc
     2641 gcacggcgcg cgggcccggc ggcgccttcc gcgcgctgca ggccgaccgc tacgaacggc
     2701 ggctgctgcg cgcgttcgac ggcgcggtcg cgctgtcggc cgccgacgct gcggcgctgg
     2761 cggtgcaggc gccatccctg cccgtcgaga tcgtgccgag cggcatcgac gtcgcggcgt
     2821 tcgcggccgg ctcgggcaac ggcgcagtgt cggccgccga cccggcgacg atcgcgttcg
     2881 tcggctatta caagcacgcg ccgaacgtcg acgccgcgca gtggctggtg cacgagatcc
     2941 tgccgcgcgt gcgccaggca gtgccggcgg cgcgcgtgcg cctgatcggc cgcgatgcgc
     3001 cggccgaagt cgcagctctc gcgcgcgaag gtgcggtcga ggtgcccggc ttcgtgccgg
     3061 acctcgccgg cgcgctcgcg cacgcgacgg tggtcgcgct gccgctgcgc cagggcggcg
     3121 gactgcgcgg caagctgctc gaggcctggg ccgcgggccg gcccgtggtg acgacggccg
     3181 tcgcctgcga gggctacgag gtcgccgccg gcgagcactg cctggtcgcc gacgatgcgc
     3241 ccgcgttcgc ggcggcgctg gtgcgctgcc tgcaggagcc gctgctgcgc gactcgctgg
     3301 ggcgcgcagg ccgcgcacac tgcacagagc actactcggt cgccgccgcg gcggcgacgt
     3361 tcgacggcgt ctacgagcgg ctgtgcgcgg ggaggacgca gtgaagatcg cgctgttcct
     3421 gaccacggag ctcgggcccg cgcgcatatt gctcgacaag gtgctcgacc gccacggagg
     3481 cgaggtgccg agcgtgttcg tgcgcgacga gcaccgcgtc ccgctcaccg ccgacctgga
     3541 gcattgcgac gtgcgccgcg acaagccggc cggcggcaag ctcgc
//
LOCUS       DASRDG010000026         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_238044, whole genome shotgun sequence.
ACCESSION   DASRDG010000026 DASRDG010000000
VERSION     DASRDG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238044"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..2519
                     /locus_tag="VFA35_00375"
     CDS             42..2519
                     /locus_tag="VFA35_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZT54649.1"
                     /translation="MSGTHTRTAAEAAPPAIAIALISAAALAFEILLMRLFSIILWHH
                     FAYMIISLALLGYGASGTLLTLAPSAVQQRFAPLFCAAAAGFGVSAIGSFMLAQRVPF
                     NPLEILWDPRQPAYLLAVYLLLALPFLCAGACVCMAFSRWRGIVSRIYSFDILGAGAG
                     SLAVMGLLFVLSPQDALRLISALGFAAAAVAWLECGRPGRWPAAALMAMAGLPFLLPE
                     SWVEPMMSPYKGLSQTLQIPQARVVAERSSPLGLVSVVESPGTPLRYAPGLSLNATAE
                     PPPQLGVFIDGEGMAALTRFDGRRESLAHLGQMTSALPYHLLREPRVLVLGAGAGADL
                     LQARYHGARQVDAVELNPQVVDLVRRQFATYAGGIYSEGADSGMPPTVRVHVAEARGF
                     VAASEQRFDLIHVALLDSFSASSAGLYALSENFLYTVQALQDDLRHLERGGLLAITRW
                     VTLPPRDALKLFGAAVVALERSGVADPASQLALVRSWQTSTLLIKNGAFSSEDIVSIK
                     AFCIERSFDVAFYPGMKPQEANRYNVVAPPDLFDGATALLGPGRDEFLQSYKFHIAPA
                     TDDKPHFFHFFRWRSLPELLSLKDQGGLPLLEWGYPVLVATLVQAVLASLLLIAAPAA
                     WVTWSGARRPASLLHAGLRRRVLVYFAAIGLAFMFIEIAFIQKFVLFLSHPLYAVAVV
                     LFAFLLFAGIGSAASKRWHDGSAPMLRHAELAVAGAIALSAVLCLSLLPWLFQNAVAL
                     PDSVRIVISAALIAPLAFFMGMMFPMGLARVEAGDARLIPWAWATNGCASVTGAVLAT
                     LVAIHIGFTAVVVAALVLYAVAATARP"
     gene            complement(2620..>3116)
                     /locus_tag="VFA35_00380"
     CDS             complement(2620..>3116)
                     /locus_tag="VFA35_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZT54650.1"
                     /translation="VLPPRFGYAAMRELTGAALLGAPLRRLGAVFVERGDAAGGVEDT
                     AVLEAHARAGEPLVVFPEGTFRRAPGLLPFKLGAFVVAAHTGVPIIPVTLAGTRSLLR
                     DGEWLPRRGEIVITVGSPIVTTERDWAAALALRAAARSAILASVAEPDAEIAPHAAGT
                     LAGV"
BASE COUNT          420 a         1042 c         1059 g          595 t
ORIGIN      
        1 ctttcgttcc gctgaccggc aagcgctagc aggcaatccc gatgagcggg acacacacgc
       61 ggactgctgc ggaggccgcg ccgccggcaa tcgccatcgc cctgatctcg gccgcagcgc
      121 ttgcgttcga gatcctgctg atgcgactgt tctcgatcat cttgtggcac cacttcgcct
      181 acatgatcat cagcctggca ctgctcggct atggcgcgag cggcaccttg ttgaccttgg
      241 cacccagcgc cgtgcagcag cgttttgcgc cgttgttctg cgcggccgcg gctgggttcg
      301 gggtcagcgc gatcggcagt ttcatgctgg cgcagcgtgt gccgttcaat ccgctggaaa
      361 ttctctggga cccacgccag ccggcgtacc tgctggccgt ctatctcctt ctggccttgc
      421 ccttcctgtg cgccggcgcc tgcgtgtgca tggccttttc gaggtggcgc ggcatcgtct
      481 cccgcatcta cagcttcgac attctgggtg ccggtgcggg aagccttgcg gtcatgggat
      541 tgttgttcgt gctgtcgccg caggacgcat tgaggctgat cagtgccttg gggttcgccg
      601 ctgcggcggt cgcgtggctg gagtgcggcc gcccggggcg ctggccggca gcggcgctga
      661 tggccatggc aggactgccg ttcttgttgc cggaatcgtg ggtcgagccc atgatgtcgc
      721 cgtacaaggg cctgtcgcag accctgcaaa ttccccaggc acgagtcgtg gcggaacgct
      781 ccagcccgct gggcctggtc agcgttgtcg agagtccagg cactccattg cgctatgcgc
      841 cggggttgag cctcaacgca acggcagagc cgccgccgca gttgggcgtt ttcatcgacg
      901 gcgagggcat ggccgcgctg acccgcttcg acggccggcg cgagagtctg gcgcatctcg
      961 gtcagatgac ctccgcgctg ccgtaccacc tgttgcgcga gccgcgcgtg ctggtgctgg
     1021 gggcaggtgc cggtgccgac ctgctgcagg cccgctatca cggtgcccgg caggtcgatg
     1081 cggtcgagct gaacccgcag gtcgtcgacc tcgtgcgacg gcagttcgcg acctatgcgg
     1141 gcggcatcta ttcggagggc gccgattcgg gaatgccacc taccgtgcgc gtgcatgtgg
     1201 ccgaggcgcg cggcttcgtt gcagccagcg agcaacgctt tgacctcatc cacgtggcgc
     1261 tgctcgattc gttcagcgcg tcctcggccg gcctgtatgc gctgtcggag aatttcctct
     1321 atacggtgca agccctgcag gacgatctgc ggcatctgga gcgcggcggc ctgctggcga
     1381 tcacgcgctg ggtcacgctg ccgccgcgcg acgcgctcaa gctgtttggc gcggcggtgg
     1441 tggcgctgga gcgttcgggg gtggccgatc ctgcgtccca gctcgcgctc gtgcgaagct
     1501 ggcagaccag cacgctgctc atcaagaacg gcgcgttcag cagcgaggac atcgtgtcga
     1561 tcaaggcctt ctgcattgag cgctcgtttg acgtcgcttt ctatccgggc atgaagccgc
     1621 aggaagcgaa ccgctacaac gtggtcgcgc cgcccgatct gttcgacggt gcaacggcgc
     1681 tgctgggtcc ggggagagac gagttcctgc agagctacaa gttccatatc gctccagcca
     1741 ccgacgacaa gccgcacttc ttccacttct tcaggtggcg atcgctgccg gaactgttgt
     1801 cgctgaagga ccaaggcggc ttgccgctgc tcgaatgggg atatccggtg ctcgtggcca
     1861 cgcttgtcca ggcggtgctc gcgagcctgt tgttgatcgc agcgcccgcg gcctgggtca
     1921 cgtggtccgg cgcccggcgg cccgccagcc tgctccacgc cggattgcgc cgccgcgtcc
     1981 tcgtctactt tgcggcgatc ggcctcgcct tcatgttcat cgagatcgcg ttcatccaga
     2041 agttcgtcct tttcctgagc cacccgctct acgcagtggc agtcgtgctg ttcgcgtttc
     2101 tgctgttcgc cggaatcggc agtgccgcct ccaagcgttg gcacgacggc agcgcgccga
     2161 tgctgcggca cgcggagctt gcggtcgcgg gagcgattgc actgtcggcg gtgctttgcc
     2221 tgagcctgct tccctggctg ttccagaacg cggttgcgct gccggactca gtgaggatcg
     2281 tgatctcggc cgcactcatt gcgccgctgg cgttcttcat ggggatgatg tttcctatgg
     2341 ggctggcgag ggtggaggcg ggcgatgccc ggctcattcc ctgggcctgg gcgaccaacg
     2401 gctgcgcatc ggtcacgggc gcggtgcttg ccacgctggt tgcgattcac atcggattca
     2461 ccgccgtggt cgttgctgcg ctcgtgctgt atgcggtcgc agcgacggcc cggccttgac
     2521 gctccgcgca cgcggcgcgc cgtcatgcat tgcctgcgcc ggggacgaga ggcatccaac
     2581 tgccgcgttc gtcgtgtcac gctggccgag ccggcgcctt cacactcctg ccaacgtccc
     2641 cgccgcatgc ggagcgatct cggcatcagg ctcggccacg ctcgcgagga tcgcgcttct
     2701 ggcggcagcg cgcagtgcga gcgcggcggc ccagtcgcgt tcggttgtga cgattggcga
     2761 gccgacggtg atcacgatct cgccgcggcg cggtaaccac tcgccgtcgc gcagcagcga
     2821 gcgcgtgccg gccagagtga ccggaatgat cggcaccccc gtgtgcgctg cgacgacgaa
     2881 ggctccgagc ttgaacggca gcagtccggg cgcgcgccgg aaagtaccct cagggaagac
     2941 caccagcggt tcgccggcgc gggcatgcgc ctcgagaacc gccgtgtcct cgacgccgcc
     3001 tgccgcgtcg ccccgctcga cgaacaccgc gccgagtctg cgcagcggcg caccgagcag
     3061 cgccgcgcca gtcagctcac gcatggccgc atacccgaat cgcggcggca gcaccg
//
LOCUS       DASRDG010000027         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_238352, whole genome shotgun sequence.
ACCESSION   DASRDG010000027 DASRDG010000000
VERSION     DASRDG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238352"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VFA35_00385"
     CDS             complement(<1..1060)
                     /locus_tag="VFA35_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HZT54651.1"
                     /translation="MQYLSATYNLWGVVASVLIASFASYVTLDLAKRVHLAEGGMAVL
                     WWAGGSLAMGTGIWSMHFIGMLAFTLPIPLGYTGALTLLSWLAAVGASSVALWTAVHG
                     VLSWKRLIGGALAMGAAICAMHYTGMAGLDMSPGIVWNPWLVAASAVVAVAAAAAALR
                     IFFWLRGVSERRSVAYQVAAAVLMGLAISGMHYTGMAAAGFPAGAVCLSADALSGDAL
                     GYLVVLGSVTMLALTLFTSSVDARAQRNQRAASRLSAANAQLEAAGREVRARLEAVFE
                     HAPNGYILFDRQRGIGQCNPAAVKLFAAPGAESLSGRALWLAPLSPPNQADGRTSEEA
                     AQAHLEQQQRSGDRVLSFE"
     gene            complement(1085..1516)
                     /locus_tag="VFA35_00390"
     CDS             complement(1085..1516)
                     /locus_tag="VFA35_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZT54652.1"
                     /translation="MKILLPVDGSENALDAVRHALRLAGNGLHARYVLANVQEPASLY
                     EVVVAHSAEVIQQVSAAAAEHALAPAQALLRAAGLAFDTEIGHGDPGHQIVEIAERCG
                     CDLIILGARGMAPLEGALGSAANSVLHHAGVPVMVVRRNPG"
     gene            complement(1588..2484)
                     /locus_tag="VFA35_00395"
     CDS             complement(1588..2484)
                     /locus_tag="VFA35_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZT54653.1"
                     /translation="MTLLVGTASWSSKTLVDSGRFYPPGVASAEARLRHYASHFPLVE
                     VDSSYYAMPAPATAQRWAERTPDGFTMNVKAFRLFTGHPTSPTVLAGDIRAALPPSTK
                     ANLYLRDLPAEIADELWRRFTEALAPLRAANKLGLVHFQFPPWLLRHAEGHAHVRMCI
                     ERMAGHTLSVEFRHRSWFADARASASTLAFERELGVVHTVVDGPQGFANSVPALWEVT
                     HPQYALLRLHGRNAETWNIRGARDSSERFNYDYSEAELGELAQQIRRLAARALATHVV
                     FNNNMEDQGQRNAASLAVLLEN"
     gene            2535..>3746
                     /locus_tag="VFA35_00400"
     CDS             2535..>3746
                     /locus_tag="VFA35_00400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HZT54654.1"
                     /translation="MPMSTAARHDPFASLNDQQRSAVEHGLVEGQPIAVPGPMLVIAG
                     AGSGKTMTLAARVARLALAGADPQRILLLTFSRRAAQEMQRRVGRVLHQALGFASTQR
                     APQLHWAGTFHAIGARLLREYALQIGLAENFTIQDRADSEDLMGLVRHELKFATTKNR
                     FPLKGTCLSIYSRVLNSRQPLGELLRSSYPWCQMWEDELKQLFRAYVAEKQAQCVLDY
                     DDLLLYWSQMLAEPALAAHVSARFDHVLVDEYQDTNRLQAAILHALKPDGRGLTVVGD
                     DAQSIYSFRAAEVRNILDFPAQFEPPARVVTLERNYRSTQPILDASNAVIALAAQRFG
                     KRLWSDSPSSQRPSLTTVPDESAQAAWVADQALRHREAGIALKSQAALFRASHHSAAL
                     ELELTRRNIPFV"
BASE COUNT          567 a         1323 c         1324 g          532 t
ORIGIN      
        1 actcgaagga cagcacgcgg tcgccggagc gctgctgttg ctcgagatgc gcctgcgcgg
       61 cctcttcgct cgtgcgtcca tcggcctgat tcggcggcga cagcggcgca agccacagtg
      121 cgcgccccga caacgattcg gcgccgggcg ccgcgaagag cttgacggcg gcggggttgc
      181 actgtccgat gccgcgttgg cgatcgaaca ggatgtagcc gttcggcgcg tgttcgaaca
      241 ccgcttcgag cctggcccgt acttcccggc ccgccgcttc caactgggcg ttggccgcgc
      301 tcagcctcga ggcggcgcgc tggttgcgct gcgctcgcgc atcgacgctc gacgtgaaga
      361 gcgtgagggc gagcatggtc accgacccca gcacgacgag gtagcccaga gcgtcgccgg
      421 agagcgcgtc agcgctcagg cacaccgcgc cggcgggaaa gcccgccgcg gccatgccgg
      481 tgtagtgcat gccgctgatc gccagcccca tgagcacggc ggccgcgacc tggtaggcaa
      541 cgctgcgccg ttcactgacg ccgcgcagcc agaagaagat ccgcaacgcc gccgccgcgg
      601 cggcaacggc cacgaccgcg gaggcggcga ccagccaggg gttccagacg atgccgggcg
      661 acatgtcgag cccggccatg ccggtgtagt gcatcgcgca gatcgccgca cccatcgcca
      721 acgcaccgcc gatcaggcgc ttccagctca gcacgccgtg aacggcggtc cacagcgcca
      781 cgctcgacgc gcccaccgcc gcgagccagg acagcagcgt cagcgctccc gtgtagccga
      841 gcgggatcgg caacgtgaac gcgagcatgc cgatgaagtg catcgaccag atcccggtgc
      901 ccatcgcgag cgagccgccg gcccaccaga ggaccgccat gccgccttcc gcgagatgca
      961 cgcgcttggc cagatccagg gtcacgtagg acgcgaagct ggcgatcagc acggacgcaa
     1021 cgacgcccca gaggttgtag gtggccgaca ggtattgcat ggcgctttca gcgggccgca
     1081 gccgtcagcc cgggttgcgc cgcacgacca tcaccggcac gccggcgtga tgcagcaccg
     1141 agttcgcggc cgagccgagc gcgccttcga gcggcgccat gccgcgcgcg ccgaggatga
     1201 tcaggtcgca gccgcagcgc tcggcgatct cgacgatctg gtggcccggg tcgccatggc
     1261 cgatctcggt gtcgaacgcc aggccggcgg cgcgcagcag cgcctgcgcg ggcgcgagcg
     1321 cgtgttcggc agcggccgcg ctgacctgct ggatcacctc ggcgctgtgc gcgacgacga
     1381 cctcgtacag cgaggccggc tcctgcacgt tggccagcac gtagcgcgcg tgcaggccat
     1441 tgccggcgag ccgcagcgcg tggcgcacgg catcgagcgc attctcggag ccgtcgaccg
     1501 gcagcagaat cttcatggct gtctcctgta acgcggtagg cggagcttaa acggcccgcg
     1561 cggcgccgtg gcgcgccgtg gcgcggctta gttctcgagc aacaccgcca atgacgccgc
     1621 gttgcgctgc ccctggtctt ccatgttgtt gttgaacacg acgtgcgtgg cgagcgcgcg
     1681 cgccgcgagg cgcctgatct gctgcgcgag ctcgcccagc tcggcctccg agtagtcgta
     1741 gttgaagcgc tccgacgaat cgcgcgcgcc ccggatattc caggtctcgg cgttgcggcc
     1801 gtgcaggcgc agcagcgcgt actgcggatg cgtgacttcc cacagcgccg gcacgctgtt
     1861 cgcgaagccc tgcgggccgt cgaccacggt gtggacgacg ccgagctcgc gctcgaacgc
     1921 gagcgtcgac gcgctcgcgc gggcgtcggc gaaccagctg cggtggcgga actcgacgct
     1981 cagcgtgtgg ccagccatgc gctcgatgca catgcgcacg tgggcgtggc cctcggcatg
     2041 gcgcagcagc cagggcggga actggaagtg cacgaggccg agcttgttcg ccgcgcgcag
     2101 cggcgccagc gcctcggtga agcgccgcca gagctcgtct gcgatctcgg ccggcaggtc
     2161 gcgcaggtac aggttggcct tggtgctcgg cggcagcgcg gcgcggatgt cgcccgcgag
     2221 tacggtcggc gaggtcgggt ggccggtgaa caggcggaag gccttcacgt tcatcgtgaa
     2281 gccgtccggc gtgcgctcgg cccagcgctg cgcagtggcc ggcgcgggca tcgcgtaata
     2341 gctcgaatcg acctcgacca gcgggaagtg cgacgcgtag tggcgcagcc gcgcctcggc
     2401 gctggcgacg ccgggcgggt agaagcgccc gctgtcgacg agcgtcttgc tcgaccacga
     2461 ggcggtgccg acgaggaggg tcatgttctt cttgcgcccc ttcttgtggg ctccaagctg
     2521 gatgcaatta cagtatgccc atgtccaccg ccgcacgcca cgaccctttc gcctcgctga
     2581 acgaccagca gcgcagcgcc gtcgagcacg ggctggtcga agggcagccg atcgcggtac
     2641 ccggcccgat gctggtgatc gccggcgccg gctcgggcaa gacgatgacg ctcgcggcgc
     2701 gggtcgcgcg cctcgcgctc gccggcgccg acccgcagcg catcctgctg ctgaccttct
     2761 cgcgccgcgc cgcgcaggag atgcagcgcc gcgtcggccg cgtgctgcac caggcgctgg
     2821 gcttcgcctc gacccagcgc gcgccgcagt tgcactgggc cggcaccttc cacgccattg
     2881 gcgcgcggct gctgcgcgag tacgcgcttc agatcggcct ggccgagaac ttcacgatcc
     2941 aggaccgcgc agattcggaa gacctgatgg gcctggtgcg ccacgagctg aagttcgcga
     3001 cgacgaagaa ccgctttccg ctgaagggca cgtgcctgtc gatctactcg cgcgtgctga
     3061 acagccggca gccgctcggc gagttgctgc ggagtagcta cccgtggtgc cagatgtggg
     3121 aagacgagct gaagcagctg ttccgcgcgt atgtcgccga gaagcaggcg cagtgcgtgc
     3181 tcgactacga cgacctgctg ctgtactggt cgcagatgct ggccgagccg gcgctggccg
     3241 cgcacgtgtc ggcgcgcttc gaccacgtgc tggtcgacga gtaccaggac acgaaccgcc
     3301 tgcaggccgc gatcctgcac gcgctcaagc ccgatggccg cggcctcacg gtggtcggcg
     3361 acgacgcgca gtcgatctac tcgttccgcg ccgcggaggt gcgcaacatc ctcgatttcc
     3421 cggcgcaatt cgagccgccg gcacgcgtcg tcacgctgga gcgcaactac cgctcgacgc
     3481 agccgattct cgacgcgtcg aacgcggtca tcgcgctcgc cgcgcagcgc ttcggcaagc
     3541 ggctgtggag cgattcgccc tcctcacagc gcccgtcgct gaccacggtg cccgacgagt
     3601 cggcgcaggc cgcgtgggtg gccgaccagg cgctgcggca ccgcgaagcc ggcatcgccc
     3661 tcaagagcca ggcggcgctg ttccgggcct cgcaccacag cgcggcgctg gagctggagc
     3721 tgacgcggcg caacatcccg ttcgtc
//
LOCUS       DASRDG010000028         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1616003, whole genome shotgun sequence.
ACCESSION   DASRDG010000028 DASRDG010000000
VERSION     DASRDG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616003"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /gene="mreC"
                     /locus_tag="VFA35_00405"
     CDS             <1..850
                     /gene="mreC"
                     /locus_tag="VFA35_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZT54655.1"
                     /translation="AGASVIAEPVWRLAAAPSELARDARIALADRHALSEQNAELSRQ
                     LLLAQTRLRRVEAVQEQNQRLQELLSVQKTLGLSVQLARVIDVDFGPYKRHTIMLDLG
                     SDNGAKVGQPVIDARGLVGQIVEVRPHSCTVLLISDKSHAVPVRVERTGMRTIARGNG
                     TLDTLELPNIPTSADVKPGDKLLTSGLGGRFPADFPVGVIRSVGNDATGMFAAAKATP
                     DAALDRSAEVLVLHELANPVGPPAPAEEIGPPVSLKGNDEPAPPPVQPTSPPAAQPDP
                     TNQGEP"
     gene            850..1338
                     /gene="mreD"
                     /locus_tag="VFA35_00410"
     CDS             850..1338
                     /gene="mreD"
                     /locus_tag="VFA35_00410"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZT54656.1"
                     /translation="MRIRGLNWLFWGGIAACFVFQLAPLPAVLLPFKPYWLALVLIYW
                     MIELPERVPLGLCFVLGVLADLLGGEILGEQALRLCILCFIVLRFRSRLRFFPIWQQT
                     LAVFALLLNDRVVYLMVHAIAGDPPPPLDFWFAPVAGMLAWPWLFLLLDDLRARLRAS
                     DT"
     gene            1571..>2557
                     /locus_tag="VFA35_00415"
     CDS             1571..>2557
                     /locus_tag="VFA35_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZT54657.1"
                     /translation="MNLPGARMQVRNARIEAEMFRARAIVGFLLIVLCLGTLVSRYVF
                     LQVYRGAEFSETSDKQRISVRSVAPSRGLVYDRNGVLLADNVAAFRLEIVPEAVDGGR
                     KGMEHLLAELGDVVAISTDDIERFRNARAKKKAFESVPLRLKLSEDEIARFSVNRWRF
                     PGVEVVPYLTRAYPLGPLFSHAVGYVGRIDLNELVKLDPNVYAGATHIGKTGVERWYE
                     DELHGAPGSETVEVGADGRVRKKLDYVSPTPGKNLYLSIDARLQRAAEEALGARLGAA
                     VAIDPRNGEVLALVSEPDFDGNLFVNGIGQTDYAQLTQDPRKPLLNRAIAGGF"
BASE COUNT          394 a          910 c          838 g          415 t
ORIGIN      
        1 tgccggcgcg agcgtcatcg ccgaaccggt ctggcgcctg gccgcggcgc cgtcggaact
       61 cgcgcgcgac gcgcgcatcg ccctcgccga ccggcacgcg ctgagcgaac agaacgccga
      121 gctgagccgc caattgctgc tcgcgcagac gcgcctgcgc cgtgtcgaag ccgtgcagga
      181 gcagaaccag cgcctgcagg aactgctctc ggtgcagaag acgctcggcc tgtccgtgca
      241 gctcgcgcgc gtgatcgacg tcgacttcgg tccgtacaaa cggcacacga tcatgctcga
      301 tctcggcagc gacaacggcg ccaaggtcgg ccagcccgtc atcgacgcgc gcggcctcgt
      361 cggtcagatc gtcgaggtgc gtccgcacag ttgcacggtg ctgctgatct cggacaagtc
      421 gcacgcggtg ccggtgcgcg tcgaacgcac cggcatgcgc acgatcgcgc gcggcaacgg
      481 cacgctcgac acgctggaac tgccgaacat cccgaccagc gccgacgtga agcccggcga
      541 caaactgctg acctcgggtc tgggcgggcg cttcccggcg gattttccgg tcggcgtgat
      601 ccgctcggtc ggcaacgacg cgaccggcat gttcgccgcc gccaaggcca cgccggacgc
      661 cgcgctcgac cgcagcgccg aggtgctcgt gctgcacgaa ctcgccaacc cggtgggtcc
      721 cccggcgccg gccgaggaaa tcgggccgcc ggtatcgctc aagggcaacg atgagcccgc
      781 gccgccgccg gtgcaaccga cttcaccacc ggcggcacag ccagacccga ccaaccaggg
      841 ggagccgtaa tgcgcatccg cggtctcaac tggctgttct ggggcggcat cgccgcgtgc
      901 ttcgtgttcc agctcgcgcc gctgccggcg gtgttgctgc cgttcaagcc gtactggctc
      961 gcactcgtcc tgatctactg gatgatcgag ctgcccgagc gcgtgccgct cggcctgtgc
     1021 ttcgtgctcg gcgttctcgc cgatctgctc ggcggcgaga tactcggcga acaggcgctg
     1081 cgactttgca tactctgctt catcgtgctg cgctttcgct cgcgcctgcg cttcttcccg
     1141 atctggcagc agacgctcgc ggtgttcgcg ctgctgctca acgaccgcgt cgtctatctc
     1201 atggtgcacg cgatcgccgg cgacccgccg ccgccgctcg acttctggtt cgcgcccgtg
     1261 gccggcatgc tcgcctggcc gtggctgttc ctgctgttag atgatctacg cgcgcgcctg
     1321 cgcgccagcg acacatgagc ccgtccaaaa acctactgcg ctcgagaatt tgcgcgccgg
     1381 cggtgctcgg actgcgcagg caggatgccg aagcgaacat tcgcgcagcg gatggcccgc
     1441 agggcgagtc gcggatgcgg cgagtcatcc tcacatactc atttgtacac tccggttcct
     1501 gcgctccggc gccgcgcccg attctagata agatcggctc ttcgctcgct acgctttttg
     1561 gacgggctca atgaacctgc ccggcgcacg catgcaggtc cgcaacgccc gcatcgaagc
     1621 ggaaatgttt cgtgcgcggg ccatcgtcgg cttcctgctc atcgtgctct gcctcggcac
     1681 gctggtgtcg cgctacgtgt tcctgcaggt ctatcgcggc gccgagttca gcgaaacctc
     1741 ggacaagcag cgcatctcgg tgcgcagcgt ggcgccgagc agaggtctcg tctatgaccg
     1801 caacggcgta ctgctcgccg acaacgtcgc cgcgttccgc ctcgagatcg tgcccgaggc
     1861 ggtcgacggc gggcgcaagg gcatggagca cctgctcgcc gaactcggcg acgtcgtcgc
     1921 gatcagcacc gacgacatcg agcgcttccg caacgcgcgc gcgaagaaga aggcgttcga
     1981 gagcgtgccg ctgcgcctca agctcagcga agacgagatc gcgcgcttct cggtcaaccg
     2041 ctggcgcttt cccggcgtcg aggtggtgcc gtacctgacc cgtgcctatc cgctggggcc
     2101 gctgttctcg catgcggtcg gctacgtcgg ccgcatcgac ctgaacgaac tggtcaagct
     2161 cgacccgaac gtctatgccg gcgccacgca catcggcaag accggcgtcg agcgctggta
     2221 cgaggacgag ttgcacggcg cgccgggcag cgaaacggtc gaggtcggcg ccgacggccg
     2281 cgtgcgcaag aagctcgact acgtatcgcc gacgccgggc aagaacctct acctgtcgat
     2341 cgatgcgcgc ctgcagcgtg ccgccgagga ggcgctcggc gcgcgtctcg gcgcggcggt
     2401 cgcgatcgat ccgcgcaacg gcgaagtgct cgcgctggtc agcgagcccg acttcgacgg
     2461 caacctgttc gtcaacggca tcggccagac cgactatgcg cagctgacgc aggacccgcg
     2521 caagccgctg ctcaaccgcg cgatcgccgg cggcttc
//
LOCUS       DASRDG010000029         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1506753, whole genome shotgun sequence.
ACCESSION   DASRDG010000029 DASRDG010000000
VERSION     DASRDG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506753"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFA35_00420"
     CDS             complement(<1..588)
                     /locus_tag="VFA35_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HZT54658.1"
                     /translation="MTSSRFVPRAAFCAGLLVPALCLAHVGNDSGGHHGAAVAGFMHP
                     FTGLDHLAAMLALGVWSALTARRVWLAPLVFAATLAVGALIGLGGSTGFWLPSVEPMI
                     AASLLVLGLLLATGAKLPALGGAAIAAVFALFHGAAHGQELAGHGALAAVAGMVVATA
                     LLHSAGIGLGLALKQRSVWLPRAAGAAVALFGVTLL"
     gene            complement(604..906)
                     /locus_tag="VFA35_00425"
     CDS             complement(604..906)
                     /locus_tag="VFA35_00425"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HZT54659.1"
                     /translation="MELTPREKDKLLIFTAALLAERRKARGLKLNYPEAVALISAAIM
                     EGARDGKSVAALMSEGKTWLTRADVMDGVAELIPEIQMEATFPDGTKLVTVHQPIA"
     gene            1068..>4055
                     /locus_tag="VFA35_00430"
     CDS             1068..>4055
                     /locus_tag="VFA35_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT54660.1"
                     /translation="MAVQQVIKVRRDYNSWVARETMEDYALRFTPRSFRKWSALRVAN
                     TAFGAASFLVLEAVGGTLLVDYGFVNAFWAILATGLIIFLAGWPISVYAARHGVDMDL
                     LTRGAGFGYIGSTITSLIYASFTFIFFALEAAIMAYAIELAFDIPPAWGYLLCSLVVI
                     PLVTHGVTTISRLQVWTQPLWLLMLIVPLAYVLHQHPGVLGELWQYGGQAPDGTPRAP
                     GFDLLKFGAAMTVGIALITQMGEQADYLRFMPEPVPGRQARWWAGVIIGGPGWVVPGV
                     LKMLAGALLAYLALGYAVPADRAVDPTQMYLVAYNAVFSQYGLAVAATALFVFVSQLK
                     INVTNAYAGSLAWSNFFARLTHSHPGRVVWMVFNTAIALMLMELNVFQALGKVLGLYS
                     NIAISWMMAVVADLVINKPLGWSPKGIEFKRAHLYDVNPVGVGAMGVASLLSIVAYLG
                     ALGPLAQAFSALIALVTALIVSPLIAWWTKGRYYLARSPVVVPAGDDGSYKRLTRCVI
                     CERDYERDDLAHCPAYQGDICSLCCTLDARCNDLCKPHARLSAQWNQLLRTLLPRVAW
                     PYIETGLGHYLLLMAVVVPLLAGLFATLYAQELPALLEAGGAGAAALRLGFVKAFAAL
                     LLASGIVAWWLVLTHKSRQVAQEESNRQTQALNEQTEALKREIESHRRTDAELQRAKR
                     SAEQANQAKSRYITTVSHELRTPLNSILGYAQLLEEDASVPKHRHQAVKVIRRGGEHL
                     LSLIEGTLDIARIEGGRLRLENKPMRFRDCVQEIVRMFELQAAAKGIEFRRVLSRPLP
                     AVVRADEKRLRQILINVLGNAVKFTQVGRVEFRLTYAREIAVFEIEDTGPGIAADEIE
                     HVFEPFARGSEASAVASGGTGLGLTIGKMLTDLMGGEMTVSSEVAGPPGSPGSGASGT
                     LFRIRLFLPEVSGAGVARELPRVVRSGYVGARKRVLVVDNEEVDRDLLANLLEPLGFE
                     IVKAASGHACLELLQQAAPGLAPD"
BASE COUNT          600 a         1408 c         1427 g          620 t
ORIGIN      
        1 cagcaacgtg acgccgaaca gcgcgaccgc ggcgccggcc gcgcgcggca accagacgct
       61 gcgctgcttc aacgccaggc cgaggccgat gccggcgctg tgcagcaacg cggtcgcgac
      121 caccatgccg gcgaccgcgg ccagcgcgcc gtggccggcc agttcctgac cgtgtgccgc
      181 accgtggaac agcgcgaaca cggccgcgat ggccgcgccg ccgagcgcgg gcagtttcgc
      241 gcccgtcgcg agcagcaggc cgagcaccag cagcgaggcc gcgatcatcg gctcgacgct
      301 gggcagccag aagccggtcg agccgcccag cccgatcagc gcgccgaccg cgagtgtcgc
      361 ggcgaacacc agcggcgcga gccagacacg ccgagcggtc aacgcgctcc acacgccgag
      421 cgcgagcatc gcggccaggt gatccagccc ggtgaacggg tgcatgaagc cggcgaccgc
      481 cgcgccgtga tgcccgccgc tatcgttgcc gacgtgcgcg aggcacagcg caggcaccag
      541 caagccggcg cagaacgcgg cgcgggggac aaaacgggaa gaagtcatcg gacggctccg
      601 gagtcacgca atcggttggt gcacggtcac caatttggtg ccgtcgggga aagtcgcttc
      661 catctggatc tcggggatca gctcggccac gccgtccatc acgtcggcgc gcgtgagcca
      721 ggtcttgccc tcgctcatca gcgcggccac gctcttgccg tcgcgcgcgc cttccatgat
      781 cgcggcgctg atcagcgcca cggcctcggg atagttcagc ttcagcccgc gcgccttgcg
      841 ccgctcggcg agcagcgcgg cggtgaagat cagcagcttg tccttctcgc gcggggtcag
      901 ttccatgggt ggcacagata gcaggaggtg tgccggatgc taggcgagcg catgcggtgc
      961 ttggcatggg gcttgcacca acgtgggtca tgagcagcgt gttgccactc gccgacgacg
     1021 ccacacgccc gcgcgtgggg caggccgtgg cggcgccagc gggcgcgatg gccgtccagc
     1081 aagtcatcaa ggtccggcgc gactacaaca gctgggtcgc gcgcgagacg atggaggact
     1141 acgcgctgcg cttcacgccg cgctcgttcc gcaagtggtc ggcgctgcgc gtggcgaaca
     1201 ccgcgttcgg cgccgcctcg ttcctcgtgc tcgaagccgt cggcggcacc ttgctggtcg
     1261 actacggctt cgtcaacgcg ttctgggcca ttctcgcgac cgggctgatc atcttcctgg
     1321 ccggctggcc gatcagcgtc tacgcggcgc gccacggcgt cgacatggac ctgctgacgc
     1381 gcggcgccgg cttcggctac atcggctcga cgatcacctc gctgatctac gccagcttca
     1441 ccttcatctt cttcgcgctc gaggcggcga tcatggccta cgcgatcgag ctcgcgttcg
     1501 acatcccgcc ggcctggggc tacctgctgt gttcgctggt cgtgatcccg ctggtcacgc
     1561 acggcgtcac gacgatcagc cggctgcagg tctggaccca gccgctgtgg ctgctgatgc
     1621 tgatcgtgcc gctggcctat gtgctgcacc agcacccggg cgtgctcggt gagctttggc
     1681 agtacggcgg gcaggcgcct gacggcacgc cgcgcgcacc cggcttcgac ctgctgaagt
     1741 tcggcgccgc gatgacggtg ggcatcgcgc tgatcacgca gatgggcgag caggccgact
     1801 acctgcgctt catgcccgag cccgtgcccg gccgccaggc gcgctggtgg gccggcgtga
     1861 tcatcggcgg ccccggctgg gtcgtgcccg gcgtgctgaa gatgctggcg ggcgcgctgc
     1921 tcgcgtacct cgcgctcggc tacgcggtgc ccgccgaccg cgcggtcgac ccgacgcaga
     1981 tgtacctcgt cgcctacaac gccgtgttct cgcagtacgg cctggcggtc gcggcgaccg
     2041 cgctgttcgt gttcgtctcg cagctcaaga tcaacgtgac gaacgcgtat gccggctcgc
     2101 tcgcgtggtc gaacttcttc gcgcggctca cgcacagcca cccggggcgc gtggtctgga
     2161 tggtgttcaa caccgcgatc gcgctgatgc tgatggaact caacgtgttc caggcgctcg
     2221 gcaaggtgct cgggctgtac tcgaacatcg cgatcagctg gatgatggcg gtcgtcgccg
     2281 acctggtgat caacaagccg ctgggctggt cgccgaaagg catcgagttc aagcgcgcgc
     2341 acctgtacga cgtgaacccg gtcggcgtcg gcgcgatggg cgtggcctcg ctgctgtcga
     2401 tcgtcgccta cctcggcgcg ctcgggccgc tggcgcaggc gttctcggcg ctgatcgcgc
     2461 tggtcacggc gctgatcgtc tcgccgctga tcgcgtggtg gacgaaagga cgctattacc
     2521 tcgcgcgttc gccggtcgtc gtgcctgccg gcgacgatgg cagctacaag cgcctgaccc
     2581 gctgcgtgat ctgcgagcgc gactacgagc gcgacgacct cgcgcactgc ccggcgtacc
     2641 agggcgacat ctgctcgctg tgctgcacgc tcgacgcgcg ctgcaacgac ctgtgcaagc
     2701 cgcacgcgcg cctgtcggcg caatggaacc agctgctgcg caccttgctg ccgcgcgtcg
     2761 cgtggcccta catcgagacc ggcctcggcc actacctgct gctgatggcg gtcgtcgtgc
     2821 cgctgctcgc cggcttgttc gcgacgctgt acgcgcagga gctgccggcg ctgctcgaag
     2881 ccggcggtgc cggcgccgcg gcgctgcggc tcggcttcgt caaggccttc gcggcgctgc
     2941 tgctggcgag cggcatcgtc gcgtggtggc tggtgttgac gcacaagagc cggcaggtcg
     3001 cgcaggagga gtcgaaccgg cagacccagg cgctgaacga gcagaccgag gcgctcaagc
     3061 gcgagatcga atcgcaccgc cgcaccgatg ccgagctgca gcgcgccaag cgcagtgcgg
     3121 aacaggcgaa ccaggccaag agccgctaca tcacgaccgt cagccacgaa ttgcgcacgc
     3181 cgctgaacag catcctcggc tatgcgcagc tgctcgaaga agacgcctcg gtgcccaagc
     3241 atcggcacca ggcggtgaag gtgatccgcc gcggcggcga gcacctgctc tcgctgatcg
     3301 aaggcacgct cgacatcgcg cgcatcgagg gcggccgcct gcggctcgag aacaagccga
     3361 tgcgctttcg cgactgcgta caggagatcg tgcgcatgtt cgagctgcag gccgcagcca
     3421 agggcatcga gtttcgccgc gtgctgagcc ggccgctgcc ggcggtggtg cgtgccgacg
     3481 agaagcggct gcggcagatc ctgatcaacg tgctgggcaa cgcggtcaag ttcacgcaag
     3541 tcgggcgggt cgagttccgg ctcacgtatg cgcgcgagat cgcggtgttc gagatcgagg
     3601 acaccggccc gggcatcgcc gccgatgaga tcgagcacgt tttcgagccg ttcgcgcgcg
     3661 gctccgaggc gagcgcggtg gcgagcggcg gcaccggcct gggcctgacg atcggcaaga
     3721 tgctgaccga cctgatgggc ggcgagatga cggtgagcag cgaggtggcg ggtccaccag
     3781 gctcgcccgg ctcgggcgcg tcgggcacac tgttccgcat ccgcctgttc ctgcccgagg
     3841 tcagcggcgc cggcgtcgca cgcgagctgc cgcgcgtcgt gcgcagcggc tacgtcggcg
     3901 cgcgcaagcg cgtgctcgtg gtcgacaacg aagaggtcga tcgcgacctg ctcgcgaacc
     3961 tgctcgagcc gctcggcttc gagatcgtca aggccgcgtc cggccacgcc tgcctcgagc
     4021 tgctgcagca ggccgcgccc ggcctcgcgc ccgac
//
LOCUS       DASRDG010000030         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_810634, whole genome shotgun sequence.
ACCESSION   DASRDG010000030 DASRDG010000000
VERSION     DASRDG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810634"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFA35_00435"
     CDS             complement(<1..732)
                     /locus_tag="VFA35_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54661.1"
                     /translation="MGHYPPGSAAAEFPYWITGLAVPSDMLLTKAWVAPAVAVLGAAI
                     FDRAAWRRLRPDWLDAPVLLWCLWPLAQGLTATDPRPAPVIASLYLIGCWGLPWLLGR
                     MYAATAEGQRLLAFGLAASALACLPFSLIEGVLGPVVYGWTYELHPFRFDGAARYLGY
                     RPLGFFENGNQFGLWVSLCALAAVWLAVSGASRLAPNVARALAAAAVSIAVAAQSIGG
                     LLLFAIGAGFLSTCRLLSPRRVVTAT"
     gene            724..1419
                     /locus_tag="VFA35_00440"
     CDS             724..1419
                     /locus_tag="VFA35_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54662.1"
                     /translation="MAHREQDPPTEEHDEHRQRRSADAEYREPAQHECQIDEFDQVHA
                     HAQGPDNTPRAADPHAKGLGTVTDAWGTNPTQVTGLVAFAVAAVACMRAAHWRARPWW
                     GLMAAQWGCFAEILFTMRFRIHDAVDATLQARHLYAQRTLWQIALLMLAVALAAACLW
                     AAWRWRRRDPEAAWAFVGTIVAVTLFVTESVSLHAVDTVMYTPMGPIVAIAAGWAAAA
                     AIVTVSALRAARH"
     gene            complement(1444..1962)
                     /locus_tag="VFA35_00445"
     CDS             complement(1444..1962)
                     /locus_tag="VFA35_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006757678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT54663.1"
                     /translation="MSPPTPFTLRSAELRDVGAIVQLIRDLAEFERLTHLLQVTPEKL
                     RPQLFGERPAAEALVAELGDGKRGGEVVAFALFFTNFSTFLAQPGLYLEDLYVKPEHR
                     GAGIGRALLTKLAALAVERGCGRFEWSVLDWNENAIRFYEGMGATVMAEWRICRVAGD
                     KLSALAEVPSRD"
     gene            2034..>2706
                     /locus_tag="VFA35_00450"
     CDS             2034..>2706
                     /locus_tag="VFA35_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZT54664.1"
                     /translation="MSSADVYEVKRPSRSRFITVRGLRHHVLEWGDASMVTPERPPLL
                     MQHGWMDVAASFQFVVDALPADRHVLAFDWRGFGLTDAPAADTYWLPDYLGDLDHALD
                     ALFFDAAPDRPIDLLGHSMGGNVVMLYAGLRPRRIRRLVNLEGFGMPATRPSQSPKRI
                     VHWLDELKAPAELRDYASLADVAARLRKTNPLLPPQRAAWLAQHWSRQRADGRFEILA
                     DPVHKR"
BASE COUNT          439 a          990 c          929 g          348 t
ORIGIN      
        1 cgttgccgtg accacgcgcc tcgggctcag cagccggcag gtcgacagga agcctgcacc
       61 gatggcgaac agcagcagtc cgccgatcga ctgcgccgcg accgcgatcg agaccgcggc
      121 ggcggccagt gcgcgcgcca cgttcggcgc gaggcgggag gctccggaca ccgccagcca
      181 cacggcggcc aacgcacaca gcgacaccca caggccgaac tggttgccgt tctcgaagaa
      241 gccgagcggc cggtacccga ggtaccgcgc cgcgccatcg aaccggaacg gatgcaattc
      301 gtaggtccag ccatacacga cgggtccgag cacgccctcg atcaggctga acggaagaca
      361 ggccagcgcg ctggcggcga gcccgaacgc cagcaggcgc tggccctcgg ccgtggcggc
      421 gtacatgcgg ccgagcaacc acggcagccc ccaacagccg atcaggtaga ggctcgcgat
      481 caccggcgcc ggccgcggat cggtggcggt cagcccctgc gcgagcggcc acaggcacca
      541 cagcagcacc ggggcatcca gccagtcggg ccgcaggcgt cgccaggccg cgcggtcgaa
      601 gatcgccgca ccaagcacag cgaccgcagg ggcgacccag gccttggtca gcagcatgtc
      661 ggacggcacc gccagaccgg tgatccaata ggggaactcg gcggcggccg agccgggcgg
      721 gtagtggccc accgggagca ggacccaccc accgaggaac acgatgaaca ccgccagcgc
      781 cggtcggcag atgcggaata ccgcgagccc gcacaacatg aatgccagat cgacgagttc
      841 gaccaggtcc atgctcatgc gcaaggaccg gacaacaccc cgcgcgccgc cgacccgcac
      901 gccaaagggc tcggcacggt gaccgacgcg tgggggacga acccgaccca ggtcaccggc
      961 ctggtcgcct tcgcagtcgc cgctgtggcc tgcatgcgcg cagcgcattg gcgtgcccgc
     1021 ccgtggtggg ggctgatggc agcacagtgg ggctgcttcg ccgagattct gttcacgatg
     1081 cgatttcgca tccacgacgc ggtcgatgcc acgctgcagg cacgccacct gtatgcgcaa
     1141 cgaacgctat ggcagatcgc gctgctcatg ctggcggtgg cactggctgc ggcttgcctc
     1201 tgggccgcgt ggcgctggcg ccgccgcgac ccggaagcgg cgtgggcatt cgtcggcacc
     1261 atcgtcgcgg tgaccctgtt cgtcaccgag agcgtctcgc tgcacgccgt cgacacggtg
     1321 atgtacacgc cgatgggccc gatcgtcgcg atcgccgccg ggtgggcagc agcagccgca
     1381 atcgtcacgg tgtcggcgtt gcgggccgcg cgccactgag cccggggcaa cgcggtcggg
     1441 cgctcaatca cgcgacggca cttccgccaa ggccgagagc ttgtcgcccg caacccggca
     1501 aatgcgccac tccgccatca ccgtcgcccc catgccttcg tagaagcgga tcgcgttctc
     1561 gttccagtcg agcacgctcc actcgaagcg gccgcaaccg cgctcgaccg cgagcgcggc
     1621 cagcttcgtc agcagcgcgc ggccgatgcc ggcgccgcgg tgctcgggct tgacgtagag
     1681 gtcttcgagg tagagcccgg gctgcgccag aaaggtcgag aagttggtga agaacagcgc
     1741 gaacgcgacg acctcgccgc cgcgctttcc atcgcccaac tccgccacca gcgcctcggc
     1801 cgccgggcgc tcgccgaaga gttgcgggcg cagcttctcg ggcgtgacct gcagcaggtg
     1861 cgtgagccgc tcgaactcgg ccagatcgcg gatcagctgc acgatcgcgc cgacatcgcg
     1921 cagctcggcg ctgcgcagcg tgaaaggggt cggggggctc atcgcctcat tctcgcatcg
     1981 gcctgtcgcg gtgccgacag cgcacgcggc gggtgccgcg tacagtgccg cgcatgagtt
     2041 ccgccgatgt ctacgaggtc aagcgcccga gccgcagccg cttcatcacc gtgcgcggcc
     2101 tgcgccacca cgtgctcgaa tggggcgatg cctcgatggt cacacccgag cggccgccgc
     2161 tgctgatgca gcacggctgg atggacgtgg ccgcgtcgtt ccagttcgtc gtcgacgcgc
     2221 tgccggccga ccggcacgtg ctcgcgttcg actggcgcgg cttcggcctc accgacgcgc
     2281 cggccgccga cacctactgg ctgcccgact acctcggcga cctcgaccac gcgctcgatg
     2341 cgctgttctt cgatgcggcc ccggatcggc cgatcgacct gctcggccac agcatgggcg
     2401 gcaacgtcgt gatgctgtac gccgggctgc gcccgcggcg catccgccgg ctggtcaacc
     2461 tcgaaggttt cggcatgccg gccacccggc cgtcgcagtc gccgaagcgc atcgtgcatt
     2521 ggctcgacga actgaaggcg cccgccgagc tgcgcgacta cgcgagcctg gccgacgtgg
     2581 ccgcgcggct gcgcaagaca aacccgctgc tgccgccgca gcgcgcggcg tggctggcgc
     2641 agcattggtc gcgccagcgc gcggacggcc gcttcgagat cctcgccgac ccggtccaca
     2701 agcgcg
//
LOCUS       DASRDG010000031         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_700091, whole genome shotgun sequence.
ACCESSION   DASRDG010000031 DASRDG010000000
VERSION     DASRDG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700091"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFA35_00455"
     CDS             complement(<1..564)
                     /locus_tag="VFA35_00455"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZT54665.1"
                     /translation="MPQDKKVALITGITGQDGAYLAEFLLKKGYIVHGIKRRTSMFNT
                     ARIDHLYQDPHVDNRRFILHFGDLTDSTNLIHLVQKIKPDEIYNLAAQSHVAVSFEEP
                     EYTANADGLGTLRLLEAMRVAGIEKTARFYQASTSELYGLVQETPQRETTPFYPRSPY
                     AVAKLYAYWITVNYREAYGMYACNGILF"
     gene            complement(770..1150)
                     /locus_tag="VFA35_00460"
     CDS             complement(770..1150)
                     /locus_tag="VFA35_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HZT54666.1"
                     /translation="MNIQPVTAGPDASPSAHLELLRVLEQHPEYSQRQLAVALGVSLG
                     KTHYLLKALLGKGWVKAQNFRRNDHKLGYLYVLTPQGVRHRFQLTQAFLVRKEEEFQT
                     LTQQIATLRRELATHDKASVDDVC"
     gene            complement(1217..1777)
                     /gene="rfbC"
                     /locus_tag="VFA35_00465"
     CDS             complement(1217..1777)
                     /gene="rfbC"
                     /locus_tag="VFA35_00465"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HZT54667.1"
                     /translation="MNVIKTAIEGVLILEPKVFGDARGFFMESFNQQTFNEAVGHEVP
                     FVQDNHSRSSKGVLRGLHYQLPPHAQGKLVRVTQGSVFDVAVDIRQGSATFGKWVGIE
                     LNGENHRQLWLPPGMAHGFLVTSESADFLYKTTDYYAPQAEGCVLWSDPALGIAWPDV
                     GVPPQLATKDAAAPPLVHAKVFEAGG"
     gene            1880..>2481
                     /locus_tag="VFA35_00470"
     CDS             1880..>2481
                     /locus_tag="VFA35_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZT54668.1"
                     /translation="MIENSIENMVENGAPSAEALPHDVQATAPPDTAVPVSLRFDSVG
                     LSESLQRAVAAQGYTTMTPIQAKAIPIVLAGRDVMGAAQTGTGKTAAFSLPLLHRMLR
                     HENASMSPARHPVRALVIAPTRELADQVAANIKGYAAQTKLRVAVVYGGIDMKPQTLE
                     LKGGVEVLVATPGRLLDHIEAKNCVLNQVEYVVLDEADRML"
BASE COUNT          452 a          759 c          802 g          468 t
ORIGIN      
        1 gaacaggatg ccattgcatg cgtacatgcc gtaggcctcg cggtagttca cggtgatcca
       61 ataggcgtac aacttggcca ccgcgtacgg gctacgtgga tagaacggcg tggtctctct
      121 ttgcggtgtt tcctgaacca agccgtagag ttcgctggtc gatgcttggt agaaacgggc
      181 cgtcttttca atgccggcca cgcgcatcgc ttcgagaagg cgcaaggtac ccaagccatc
      241 agcgttggcg gtgtactcgg gctcttcgaa gctcacggcc acgtggctct gagcagccag
      301 gttgtagatc tcgtcaggct tgatcttctg caccagatga atcaagttcg tactgtccgt
      361 cagatcaccg aagtgcagaa tgaaacgcct gttatcgacg tgaggatcct ggtagaggtg
      421 gtcgatgcgc gcggtattga acatcgacgt gcggcgcttg atgccgtgga cgatgtaacc
      481 ctttttgagt aggaactcgg ccaggtaggc gccatcctgg ccggtgattc cggtgatgag
      541 agcgactttc ttgtcttggg gcatggtctt tggttcttgg agagagctca ggcgtcaatg
      601 gccgcacaca cgcgctggat ctccgactcg aagtcgaacg tggtgcgcgt gacacttggg
      661 ttcagcgcgc aaagcgtgat cagccgtaaa gcggccagca aatccagttc ggcgcgtgcg
      721 ggccacgatg ttctcgaaga tatttgcgtc gggtctcatg atcgccggat cagcagacat
      781 catcgacgct cgccttgtcg tgagtagcca gttccctgcg caaggtcgca atctgctgcg
      841 tcagggtttg aaattcttcc tccttgcgca ccaggaatgc ctgggtaagc tgaaagcgat
      901 gccggacgcc ttgaggtgtg agtacgtaca gatacccaag cttgtggtca ttgcgccgga
      961 agttctgtgc cttgacccag ccctttccca gcagcgcctt caacaggtaa tgcgtctttc
     1021 ccaggctcac gcccagggcg acggccaatt ggcgctgcga gtactcgggg tgctgctcca
     1081 gcacgcgcag caattccagg tgagccgaag gactcgcatc ggggccggcg gtgacgggct
     1141 gtatgttcat gcgttgaacg cgtgattcta catgggccgt accggctcgc cgcgacgcgg
     1201 ccgcacacgc ctgtcgtcag ccgccggctt cgaaaacctt ggcgtgcacc agtggcggtg
     1261 ccgcggcgtc cttcgtggcc aattgcggcg gtaccccgac gtcgggccac gcgattccca
     1321 gcgccgggtc cgaccacagc acgcaccctt cggcctgcgg cgcgtagtag tcggtggtct
     1381 tgtacaggaa gtcggcggat tcgctggtga cgagaaagcc gtgcgccatt ccggggggca
     1441 gccacagctg gcgatggttc tcgccgttca gctcgatacc aacccatttc ccgaacgttg
     1501 cgctaccttg ccgtatgtcg accgccacat cgaacacgct cccctgcgtg acccgcacca
     1561 gcttgccctg tgcatgcggc ggcaactggt agtgcagccc gcgcagcacg cccttgctcg
     1621 accgcgaatg gttgtcctgc acgaacggga cctcgtggcc caccgcctca ttgaaggtct
     1681 gctggttgaa gctctccatg aagaagccgc gcgcgtcgcc gaacaccttg ggttcgagga
     1741 tgagcacgcc ttcgatggcg gtcttgatca cgttcatggg ggatgcccga gcttgacgga
     1801 gcccgcattc tagtgaggcg cgccggggcg cccggcacgc gaaccccgga cgggcgataa
     1861 tcgaaggatg accgaaaaca tgatagagaa ctcgatcgag aacatggtag agaacggcgc
     1921 accgagcgcc gaggcactgc cgcacgatgt gcaggccact gcgccgccag ataccgccgt
     1981 gcccgtttcg ctgcgattcg acagcgtcgg cctctccgaa agcctgcagc gcgccgtcgc
     2041 cgcgcagggc tacacgacga tgacgccgat ccaggccaag gcgatcccga tcgtgctggc
     2101 cgggcgcgac gtgatgggcg cggcgcagac cggcacgggc aagaccgcgg cgttctcgct
     2161 gccgctgctg caccgaatgc tgcgccacga gaacgccagc atgtcgccgg cgcgccaccc
     2221 ggtgcgcgcg ctggtgatcg cgccgacgcg cgagttggcc gatcaggtcg ccgccaacat
     2281 caagggctac gcggcgcaga ccaagctgcg ggtcgcggtc gtctacggcg gtatcgacat
     2341 gaagccgcag acgctcgagc tgaagggcgg tgtcgaggtg ctggtggcca cgccgggccg
     2401 gctgctggac cacatcgagg cgaagaactg cgtgctgaac caggtcgagt acgtcgtgct
     2461 cgacgaggcc gaccgcatgc t
//
LOCUS       DASRDG010000032         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_465732, whole genome shotgun sequence.
ACCESSION   DASRDG010000032 DASRDG010000000
VERSION     DASRDG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465732"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VFA35_00475"
     CDS             complement(<1..778)
                     /locus_tag="VFA35_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZT54669.1"
                     /translation="MRIPSTAGAQIVERSRSQEVPFCSSGRCKGDTVMQTKPLLAATI
                     ALALSASAAHADDWLQFGYDSIHSGTNFNEKTLSQNNINQAILRYRTQVSGINGSGID
                     GQLLYLNGVNTAAGKKNLLFAVASNGTVLALDAANGNLVWSVGKYAVSSSTIWSVSTP
                     ALDPNRQYLYAMALQGYLHKYRVADGSEVTGGGWPQLVSGKVDVERVSAGLTVGSSDG
                     KNTLYAVTASYNDTGNYQGHLVAIDLASGTERVQHDVQQPE"
     gene            complement(910..2367)
                     /locus_tag="VFA35_00480"
     CDS             complement(910..2367)
                     /locus_tag="VFA35_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HZT54670.1"
                     /translation="MFGAAALLTLATLAGLATGFAREWLIVAAWGSGARSDAFLVAMF
                     LPEALRMMMAGGLVNSAALPLYQEQASRRRAGWLAGQSVNLGLVGLALALALAALAPA
                     LVAVIGPGLAPAAREDAAATLRMLAWCLPGICLQALWSVPHQAASRFVRAGLASFLYN
                     LPAVCLLGFAGHATDATTLAACFVGGSVLMCVVLLPGMRAGDAAMPSATRVRRDTLAF
                     YARLAPLVASSAASQGLTLLERMAASLIGEGAVTLANLARKLVNLPLIALVSLNQVLL
                     GRMSAGIDARRRLDLLRAGLALSTLLTLPAAVAIVAAAPTLVAVLMPAGLAAGPLPNL
                     LAWYACVIVFGSWNALLARYSYAVGDTRTPLACELAGSALNAAGLLLLPHWFGLPGLA
                     LSALAGVIATGALLLRKNHLFGEVDLARESFAAALPLALAAACVHALQAAPAWQVATA
                     VALALACALAAGLRLPRLWAATREHETSSLSGAGL"
BASE COUNT          421 a          822 c          895 g          372 t
ORIGIN      
        1 gctcaggttg ctgcacatcg tgttgaacac gttcagtgcc gctggcgaga tcgatcgcga
       61 ccagatggcc ctggtagttg ccggtgtcgt tgtagctcgc agtgacggcg tagagcgtgt
      121 tcttgccgtc gctgctgccg acggtgagac cggcggacac gcgctcgaca tcgaccttgc
      181 cgctgacgag ctgcggccag ccgccgccgg tgacttcgct gccgtcggcg acgcgatatt
      241 tgtgcaggta accctgcagc gccatcgcgt agagatactg gcggttcggg tcgagcgcgg
      301 gtgttgacac ggaccatatc gtcgaactgg agacggcgta cttgccgacc gaccagacga
      361 ggttgccgtt cgccgcgtcg agcgcgagca cggtgccgtt cgaggcgacg gcgaacagca
      421 ggttcttctt gcccgccgcg gtgttcacgc cattgagata cagcagctgg ccgtcgatgc
      481 cgctgccgtt gatgccggat acctgggtgc gatagcgcag gatcgcctga ttgatgttgt
      541 tctggctgag cgtcttttcg ttgaagttgg tgccggaatg gatcgagtcg tagccgaatt
      601 ggagccagtc gtcggcgtgg gctgcggacg cgctcagggc gagggcgatg gtcgctgcca
      661 gcaagggttt cgtttgcatc acagtgtctc ctttacatct gccggatgaa caaaatggaa
      721 cctcttgaga cctactgcgc tcgacaattt gcgcgccggc ggtgctcgga atcctcatgt
      781 acctctgagt acactccgat tcctgcgctc cggcgacgca caaattttct tcgctcgcta
      841 cggtctcaaa aggctcccaa accctcgcgc gaccggcgca cgcgacggcg tgggggcgcg
      901 gccaactctt cataagccag ccccggacaa cgaagacgtt tcgtgttcgc gcgtcgccgc
      961 ccacagccgc ggcaggcgca ggcccgcggc gagcgcgcag gcgagcgcga gtgcgacggc
     1021 ggtagcgacc tgccacgccg gcgcggcctg cagcgcgtgc acgcaggccg cggcgagcgc
     1081 gagcggcagc gctgcggcga acgactcgcg cgcaagatcc acttcgccga agagatggtt
     1141 cttgcgcagc agcagagcgc cggtcgcgat cacgccggcg agtgcgctca gcgcgagacc
     1201 cggcaggccg aaccagtgcg gcaggagcag cagtcccgcg gcattgagtg cgctgccggc
     1261 gagttcgcag gcgagcggcg tgcgcgtgtc gccgaccgcg taggagtagc gcgcgagcag
     1321 cgcattccag ctgccgaaca cgatcacgca ggcgtaccac gcgagcaggt tcggcagcgg
     1381 ccccgcggcg aggcctgcgg gcatcagcac ggcgaccagc gtcggcgcgg cggcgacgat
     1441 cgcgactgcg gcgggcaagg tcagcaatgt cgacagcgcg agcccggcgc gcaagagatc
     1501 aaggcgccga cgtgcatcga tgcccgcgct catgcgtccg agcagcactt gattgaggct
     1561 gaccagcgcg atcagcggca ggttgacgag cttgcgcgcg agattggcca gcgtcaccgc
     1621 gccttcgccg atcagcgagg cggccatgcg ctcgagcaag gtcaggccct ggctcgccgc
     1681 gctgctcgcg accagcggcg cgaggcgcgc gtagaacgcg agcgtgtcgc ggcgcacgcg
     1741 tgttgccgac ggcatcgcgg cgtcacctgc gcgcatgccc ggcagcagca cgacgcacat
     1801 gagtacgctg ccgccgacga agcacgcggc gagcgtcgtc gcgtcggtgg catgacctgc
     1861 gaatccgagc aggcacaccg cgggcaggtt gtagaggaac gaagcgaggc ccgcgcgcac
     1921 gaagcgcgac gcggcctggt gcggcacgct ccacaacgcc tgcaggcaga tgcccggcag
     1981 gcaccaggca agcatgcgca acgtcgccgc ggcatcctcg cgcgctgcgg gcgcgaggcc
     2041 ggggccgatc accgcgacga gcgcgggcgc gagcgccgcc aacgcaagcg cgagcgcgag
     2101 gccgacgaga ccgagattga cgctttgccc ggcgagccag ccggcgcgtc gccgcgacgc
     2161 ctgttcctgg tacagcggca gcgcggccga attgaccagg ccgccggcca tcatcatgcg
     2221 cagcgcctcg ggcaggaaca tcgcgacgag gaacgcatcg ctgcgcgcac cgctgcccca
     2281 ggcggcaacg atgagccact cgcgcgcgaa gcccgtggcg aggccggcca gtgtcgcgag
     2341 tgtgagcagg gcggcggcgc cgaacatgat cagtggaaaa tgttgaacag gctcttgccg
     2401 cggaccttgt agtcgaggtc gcgcgggcgt tcgccgagtg ccttcgccgc gctgccgccg
     2461 acgatcttgt agggtgcgac gtccttgccg acgagcgtgt tcgcggtcac
//
LOCUS       DASRDG010000033         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1507321, whole genome shotgun sequence.
ACCESSION   DASRDG010000033 DASRDG010000000
VERSION     DASRDG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507321"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1302)
                     /locus_tag="VFA35_00485"
     CDS             complement(106..1302)
                     /locus_tag="VFA35_00485"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZT54671.1"
                     /translation="MPNAMPSCCRPLACLALLLPCLSLAAQDVLIQAKTIVVAPDTVL
                     SPGELLVRDGKVAHVGAEIPAETRSKARVVAWPDATIVPGFVLPHGTLGQDQDLAERA
                     VPMTPELLAADAFDPFRDDLQQLARNAVTSYGLAPSSRNVAAGIGALVKPGGERGRVA
                     EAQAFLKLSLAAAARDPERPPTSLMGAVDLLREGFAAAKQGAQGGPHVAALARALRGE
                     RRVFLAADSYAELTAALALAQEFAFEPALVGAASAPDCMDRIAAARAAVVLSPLLPDM
                     RGEQLLLPSRLEQRGVPFCFQGAPSQLRASAALAVHYGTSRKAALAALTRTPAVLLGQ
                     QDHVGALRRGCDADFAVFDGDPLDLGAPLRAVWIDGQRRFGTDPPPAPRTRDVAAGKV
                     AGPEVR"
     gene            complement(1308..>2023)
                     /locus_tag="VFA35_00490"
     CDS             complement(1308..>2023)
                     /locus_tag="VFA35_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZT54672.1"
                     /translation="GREQSAQAEATQEPKPAEKQEPKPAENKPQEGGAAPAQDKAPER
                     PKYPKEPAHDPVKDALLEVLDGTLPLRVEAHRPDEIRQALALAADKKVPVLVLEQPFA
                     AASCAADLANAGVACVLADVWPTQLPKPYDAYDLTALPAALQQQGVAFAIATGSGRRA
                     GALPLMAACAVGRGLDETAALRAITLTPAEILGVQNDVGSLQAGKLGDLLVTDRPLFA
                     SDCRVLAVLAAGVTQFQAK"
BASE COUNT          243 a          755 c          770 g          255 t
ORIGIN      
        1 tcggcggccc gccgccgggc cacagggtcg cacccttgta gacggtcgtc ctgtcctggg
       61 ccgccagcgc ggcggcgaac gcgaacgctg cgagcgcgcg gcgcatcagc gcacctccgg
      121 gccggcgacc ttgccggcgg cgacgtcgcg cgtgcgcggt gcgggcggcg gatcggtccc
      181 gaaccgccgt tggccgtcga tccagaccgc gcgcagcggc gcgccgaggt cgagcggatc
      241 gccgtcgaac acagcgaagt cggcgtcgca gccgcggcgc agcgcgccga cgtgatcctg
      301 ctggccgagc agcaccgcag gcgtgcgcgt gagcgccgcg agcgccgcct tgcggctcgt
      361 gccgtagtgc accgcgagcg ccgcggaggc gcgcagctgg gacggcgcgc cctggaagca
      421 gaacggcacg ccgcgctgtt cgaggcggga tggcagcagc agctgttcgc cgcgcatgtc
      481 gggcagcagc ggcgacagca cgacggccgc acgcgcggcg gcgatgcggt ccatgcagtc
      541 cggcgccgag gcggcgccga ccagcgccgg ttcgaacgcg aactcctgcg cgagcgccag
      601 cgccgccgtc agctcggcgt acgagtcggc ggcgaggaac acgcggcgct cgccgcgcag
      661 cgcccgcgcc agcgccgcca cgtgcggccc gccctgcgcg ccctgcttgg cggcggcgaa
      721 gccttcgcgc agcagatcga ccgcgcccat cagcgaggtc ggcggccgct cggggtcgcg
      781 cgccgcggcc gcgagcgaca gcttgaggaa cgcctgcgcc tcggccacgc gcccgcgttc
      841 gccgcccggc ttgaccagcg cgccgatgcc cgcggcgacg ttgcgcgacg acggcgcgag
      901 cccgtagctc gtcacggcat tgcgcgcgag ctgctgcagg tcgtcgcgga acggatcgaa
      961 cgcgtccgcc gccagcagct cgggcgtcat cggcaccgcg cgttcggcga ggtcctggtc
     1021 ctggccgagc gtgccgtgcg gcagcacgaa gccgggcacg atggtcgcgt cgggccacgc
     1081 gacgacgcgg gccttgctgc gcgtctccgc ggggatctcc gcgccgacgt gcgccacctt
     1141 gccgtcgcgc acgagcagtt cgcccggcga cagcacggtg tccggcgcga cgacgatcgt
     1201 cttggcctgg atcagcacgt cctgcgcggc gagcgacagg cacggcagca gcagggcgag
     1261 gcacgcgagc ggtcggcagc aactgggcat tgcgttcggc atgtcgttca cttcgcttgg
     1321 aactgggtca cgccggcggc aagcaccgcg agcacgcggc agtcgctcgc gaacagcggg
     1381 cggtcggtca cgagcaggtc gccgagcttg cccgcctgca gcgacccgac gtcgttctgc
     1441 acgccgagga tctcggccgg ggtcagcgtg atcgcgcgca gcgccgcggt ctcgtcgagg
     1501 ccgcggccga cggcgcaggc ggccatcagc ggcagcgcgc cggcgcggcg gccgctgccc
     1561 gtcgcgatcg cgaacgcgac gccctgctgc tgcagcgccg ccggcagcgc cgtgaggtcg
     1621 taggcgtcgt agggcttggg gagctgcgtc ggccacacgt cggcgagcac gcaggcgacg
     1681 cccgcgttcg cgaggtcggc ggcgcacgac gccgcggcga acggctgctc gagcacgagc
     1741 accggcacct tcttgtcggc ggcgagtgcg agcgcctgcc ggatctcgtc ggggcggtgc
     1801 gcctcgacgc gcagcggcag cgtgccgtcg agcacttcga gcagcgcgtc cttcaccggg
     1861 tcgtgcgcag gctccttcgg gtacttcggc cgctccggcg ccttgtcctg cgctggcgcc
     1921 gcaccaccct cctgaggctt gttctccgcc ggcttcggct cctgcttctc ggccggcttg
     1981 ggttcctgcg ttgcttctgc ctgcgcgctc tgctcacggc cga
//
LOCUS       DASRDG010000034         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_5901, whole genome shotgun sequence.
ACCESSION   DASRDG010000034 DASRDG010000000
VERSION     DASRDG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5901"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFA35_00495"
     CDS             complement(<1..698)
                     /locus_tag="VFA35_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZT54673.1"
                     /translation="MSAVVLADKVPSIVAPSISYHALLPIFIVLGGAMLGVLVEAVVP
                     RKERFATQLLVTFASLIGAGIDVCLLHNTKVVTPVAGTCPPGAKDCLASPVFGGSLAV
                     DGVGLFIQGTILALAIVATLLLAERSADVASPIVASAATVVGSPEDRRLSQAGHVQTE
                     IFPMLLFAVGGMLIFPIANNLILLFVALEVFSLPLYLMAGLARRRRLLSQKAAMKYFL
                     LGAFASAFLLYGIAL"
     gene            complement(760..>2332)
                     /locus_tag="VFA35_00500"
     CDS             complement(760..>2332)
                     /locus_tag="VFA35_00500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZT54674.1"
                     /translation="TIPILLAIPLVGSLVLFAMKPAQALAAKFLAFGVSVVVLVYTAV
                     LGFSFDPNKTCASTATNHHCFQYATSWTWIKSFGVHFAFGLDGIALVLVVMATILVPV
                     TILASWNAFDSKAGSEDGTLPQRSVPAYLGLVLLLEFFMIGVFTATDVFLFYVFFEVM
                     LVPTYFIIGSFGGPRRQYAAMKFFLYSFVGGLLMLASVIGLYIHSGTSSFAMSDLIGT
                     VPNDTTQKWLFLGFFIAFAIKAPLVPFHTWLPDAGAEAPIGGAVLLVGVLDKVGTFGF
                     LRYCIPLFPDASKYFAPVILILSVIGVLYAAFLAIGQRDMKRLVAYTSVAHFGFIGLG
                     VFAFTSQGGSGAVLYMVNHGFSTAALFFVVGFLISRGKSRNIDDYGGTAKVTPILGGF
                     FLLAGLSALALPGMSTFVSEFLVLVGTFTAHKAIAIVATTGIILAAIYVLFLYQRAFQ
                     GPVRNEKVGAFRDLNPREIAAVAPMIALIIFLGIYPKPVLDIINPAVKATLHDVGHDK
                     DPAPKYAVQANGGQK"
BASE COUNT          463 a          743 c          814 g          312 t
ORIGIN      
        1 agcgcgatgc cgtacagcag gaacgccgag gcgaaggcgc cgagcaggaa gtacttcatc
       61 gccgctttct gcgacagcag gcggcgacgg cgcgccagcc cggccatcag gtacagcggc
      121 agcgagaaca cctcgagcgc gacgaacagc aggatcaggt tgtttgcgat cgggaagatc
      181 agcatgccgc cgaccgcgaa cagcagcatc gggaagatct cggtctgcac gtggccggcc
      241 tgcgacaggc gccggtcctc gggcgagccg accacggtcg cggccgaggc cacgatcggg
      301 ctcgcgacgt cggccgagcg ctccgcgagc agcagcgtgg ccacgatcgc gagcgcgagg
      361 atcgtgccct ggatgaacag gccgacgccg tcgaccgcca gcgaaccgcc gaagaccggg
      421 ctggcgaggc agtccttggc gccgggcggg cacgtgccgg cgaccggcgt cacgaccttg
      481 gtgttgtgca gcaggcagac gtcgatcccg gcgccgatca ggctggcgaa ggtgacgagc
      541 agctgggtgg cgaagcgctc cttgcggggc acgaccgcct cgaccaggac gcccagcatc
      601 gcgccgccga gcacgatgaa gatcggcagc aatgcgtggt aagagatcga cggcgcgacg
      661 atcgacggca ccttgtcggc gaggaccacg gcactcactt cgcctcctcc gtcggcgacg
      721 gagacgtccc gatgctcatg ggctcgctcg caagctcgct cacttctgtc ctccgttggc
      781 ctggacggcg tacttcggcg ccggatcctt gtcatgaccg acgtcgtgca gggtcgcctt
      841 cacggccggg ttgatgatgt cgagcaccgg tttcgggtag atgccgagga agatgatcaa
      901 cgcgatcatc ggggcgaccg cggcgatctc gcgcgggttc aggtcgcgga acgcgccgac
      961 cttctcgttg cgcaccgggc cctggaacgc gcgctggtag aggaacagca catagatcgc
     1021 ggccaggatg atgccggttg tcgcgacgat cgcgatcgcc ttgtgcgcgg tgaacgtgcc
     1081 caccagcacg aggaactcgc tgacgaacgt gctcatgccg gggagcgcga gcgcggacag
     1141 gccggccagc aggaagaacc cgccgaggat cggcgtgacc ttggcagtgc cgccgtagtc
     1201 gtcgatgttg cggctcttgc cgcgcgagat caggaagccg acgacgaaga acagcgccgc
     1261 cgtcgagaac ccgtggttga ccatgtagag caccgcgccg gacccaccct gcgaagtgaa
     1321 cgcgaagacg ccgagcccga tgaaaccgaa gtgcgccacg gacgtgtagg cgaccaggcg
     1381 cttcatgtcg cgctgaccga tcgccaggaa cgccgcgtac agcacgccga tcaccgacag
     1441 gatcaggatc accggggcga agtacttcga cgcgtccggg aacagcggga tgcagtagcg
     1501 caggaacccg aacgtgccga ccttgtcgag cacgccgacg agcagcaccg caccgccgat
     1561 cggcgcctcg gcaccggcgt caggcagcca ggtgtggaac ggcaccagcg gcgccttgat
     1621 cgcgaacgcg atgaagaagc cgaggaacag ccacttctgc gtggtgtcgt tcgggacggt
     1681 gccgatcagg tccgacatcg cgaagctgct ggtgccgctg tggatgtaga ggccgatcac
     1741 cgaggccagc atgagcaggc cgccgacgaa ggagtacagg aagaacttca tcgccgcgta
     1801 ctgccggcgc gggccgccga agctgccgat gatgaagtac gtcggcacca gcatgacttc
     1861 gaagaagacg tagaagagga acacgtcggt ggcggtgaag acaccgatca tgaagaactc
     1921 gagcagcagg acgaggccga ggtaggcggg cacgctgcgc tgcggcagtg tcccgtcctc
     1981 cgagccggcc ttgctgtcga acgcgttcca gctcgccagg atcgttaccg gcaccaggat
     2041 cgtggccatc accacgagca cgagcgcgat gccgtccagg ccgaacgcga agtgcacgcc
     2101 gaaggacttg atccaggtcc agctggtggc gtactggaag cagtggtggt tcgtggccgt
     2161 actcgcgcag gtcttgttcg ggtcgaacga gaacccgagc acggcggtgt agaccagcac
     2221 caccacagag acgccgaacg cgaggaactt cgccgccagc gcctgcgccg gcttcatcgc
     2281 gaacagcacg agcgagccca ccagcggaat ggccagcagg atcgggatcg tc
//
LOCUS       DASRDG010000035         3633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1507511, whole genome shotgun sequence.
ACCESSION   DASRDG010000035 DASRDG010000000
VERSION     DASRDG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507511"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VFA35_00505"
     CDS             <1..129
                     /locus_tag="VFA35_00505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HZT54675.1"
                     /translation="PHAPDEFRVIGAARNLPAFGATFGVKPGDKMYLAPADRVTLW"
     gene            191..1639
                     /locus_tag="VFA35_00510"
     CDS             191..1639
                     /locus_tag="VFA35_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZT54676.1"
                     /translation="MTDVPHPHPHSRDGLAQRYGPRYRWRVLLTVMIGTMASIMASTI
                     VNVAVPDISRVFGLGQERVQWLSAGFMAAMTLSMLTTPWLLQRFGYRHTYIAVVALLL
                     AGGVIGGSSRWFELVLAMRVAEGLAAGVLQPIPAIIVMRAFERSEQGKAMGIFGFGVV
                     LAPAIGPSVGGVLVEWFGWRGVFFVVVPFCLAALVLARRYLPVSAPGGAPPERDGARL
                     DVVGLGLIAVAVLSLLNGMVQLHGAGTATGWALLACSATALVGFVFHQKRGAQPLMEL
                     RLFGHRNFAFGGVVAFIYGMALFGSTYLVPVFMQIALHMPPSQAGAALLPAGFVLALT
                     IPVAGRLTDKLPFGRIVAFGLALLAASFLLMVTVGPASSLWWIVAWAVVGRIGLGFVL
                     PSLNVGAMRGLPDAFISQGASTINFMRQLGGAVGVSLVGIVLEWRLQVHTGDPVRAFH
                     ETFVLVGLITACAMVAALQMGPSRGPAAAAAS"
     gene            1671..2912
                     /locus_tag="VFA35_00515"
     CDS             1671..2912
                     /locus_tag="VFA35_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HZT54677.1"
                     /translation="MNLGIDTLPPACDVLVIGAGPAGSGAALTLARAGLDVVLVDAQA
                     FPRDKVCGDGLIPDAHQALRKLGVLDEVMAQAQASGFVGCIGPRGGRVDVPGVLAVLP
                     RKQLDLIVCRAAVAAGARMFAPLRFTAPLEAGGRVVGATLQHGTATREVRARWVLLAT
                     GAVPQALLAAGMSSRRTPSCVALRGYVKNDAMVGRIHALEIVWHRALAPGYGWIFPCR
                     DGVFNIGVGIIDSHNARRGERLTKREVNLRQVMNDFQAVYEPARALMQGGTELGPMKG
                     APLRCTLEGARYTRPGLLVIGEAAGSTYSFTGEGIGKAFETAIHAAEALLAHGAAPAA
                     DAAVRADYEARLRALKPRFDLYQRANVVNRHPWLADLVIWRAQRSPRLVRRMSGVLNE
                     TSNPGHLLSVKGFTRLFLDRA"
     gene            2916..>3633
                     /locus_tag="VFA35_00520"
     CDS             2916..>3633
                     /locus_tag="VFA35_00520"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HZT54678.1"
                     /translation="MCQLLGMNANTPTDVMFSFTGFATRAQEHKDGFGIAFFEGAGVR
                     LLVDAQSARSSPVAEMVRRFPIHSRNIVAHIRKATQGQVSLANTHPFVRELWGRYWVF
                     AHNGDLKGFAPRLHGAFRPVGETDSERAFCWLMQELAKAHASVPTTPELTATLRELAP
                     IPAAHGTFNFMLSNGQALWAHCSTQLHHLVRQHPFAQASLQDDDVTVDFSKHTTEHDR
                     VAVIVTEPLTRNEAWVAFAPG"
BASE COUNT          445 a         1312 c         1314 g          562 t
ORIGIN      
        1 ccgcatgcgc ccgacgaatt ccgcgtcatc ggcgcggcgc gcaacctgcc ggccttcggc
       61 gcgaccttcg gcgtcaagcc gggcgacaag atgtacctgg cgccggccga ccgtgtgacg
      121 ctttggtgac ctgaggcgac gccggtcgcg cggcacaatg gtggcggcgc ctgcgagcgc
      181 cgccacccgc atgaccgacg ttccccaccc ccacccccac tcccgcgacg gcctggcgca
      241 gcgctacggc ccgcgctacc gctggcgcgt gctgctgacc gtgatgatcg gcacgatggc
      301 gtcgatcatg gcctcgacca tcgtcaacgt cgcggtgccc gacatcagcc gcgtcttcgg
      361 gctcggccag gagcgcgtgc agtggctctc ggccggcttc atggcggcga tgacgctgtc
      421 gatgctgacc acgccctggc tgctgcagcg tttcggctac cgccacacct acatcgcggt
      481 cgtcgccttg ctgctcgccg gcggcgtgat cggcggctcg agccgctggt tcgagctggt
      541 gctggcgatg cgcgtcgccg agggcctggc cgcgggcgtg ctgcagccga tcccggcgat
      601 catcgtgatg cgcgcgttcg agcgctcgga gcagggcaag gcgatgggca tcttcggctt
      661 cggcgtcgtg ctcgcgccgg cgatcgggcc cagcgtgggc ggcgtgctgg tcgagtggtt
      721 cggttggcgc ggggtgttct tcgtcgtcgt gccgttctgc ctcgcggcgc tggtgctcgc
      781 gcgccgctac ctgcctgtca gcgcgccggg cggcgccccg cccgagcgcg acggcgcgcg
      841 gctcgatgtc gtcggcctgg ggctgatcgc ggtcgcggtg ctgtcgctgc tgaacggcat
      901 ggtgcagctg cacggcgccg gtaccgcgac cggctgggcg ctgcttgcgt gcagcgcgac
      961 cgcgctggtc ggcttcgtct tccaccagaa gcgcggcgcg cagccgctga tggagctgcg
     1021 cctgttcggc caccgcaact tcgcgttcgg cggcgtggtc gcgttcatct acggcatggc
     1081 gctgttcgga tcgacctacc tggtgccggt gttcatgcag atcgcactgc acatgccgcc
     1141 gagccaggcc ggcgcggcgc tgctgccggc gggcttcgtg ctcgcgttga cgattccggt
     1201 cgccggccgg ctcaccgaca agctgccgtt cggccgcatc gtcgcgttcg gtctggcgct
     1261 gctggcggcg tcgttcctgc tgatggtcac ggtcgggccg gcgagctcgc tgtggtggat
     1321 cgtcgcgtgg gcggtggtcg ggcgcatcgg gctgggcttc gtgctgccct cgctcaatgt
     1381 cggcgcgatg cgcggcctgc ccgacgcctt catctcgcag ggcgcgagca cgatcaactt
     1441 catgcgccag ctcggcggcg cggtcggcgt cagcctggtc ggcatcgtgc tcgagtggcg
     1501 gctgcaggtg cacaccggcg acccggtgcg cgccttccac gagaccttcg tgctggtcgg
     1561 tctgatcacc gcgtgcgcca tggtcgcggc gctgcagatg ggcccatcgc gcgggccggc
     1621 tgccgctgcg gcctcctaag attcagctcc actccggccc cgtgcaccgc atgaacctcg
     1681 gcatcgacac ccttccgccc gcatgcgatg tgctcgtgat cggcgccggc ccggccggca
     1741 gcggcgcggc gctcacgctg gcgcgcgccg ggctcgacgt ggtgctggtc gacgcccagg
     1801 ccttcccgcg cgacaaggtc tgcggcgacg gcctgatccc cgatgcccac caggcgctgc
     1861 gcaagctcgg cgtgctcgac gaggtgatgg cgcaggcgca ggccagcggc ttcgtcggct
     1921 gcatcgggcc gcgcggcggg cgcgtcgacg tgccgggcgt gctcgcggtg ctgccgcgca
     1981 agcagctcga cctgatcgtg tgccgcgccg cggtggccgc gggcgcgcgc atgttcgcac
     2041 cgctgcgctt cacggcgccg ctcgaggcgg gcggccgggt cgtcggtgcg acgctgcagc
     2101 acggcacggc gacgcgcgag gtgcgcgccc gctgggtgct gctcgcgacc ggcgcggtgc
     2161 cgcaggcgct gctggcggcc ggcatgtcga gccggcgcac gccgagctgc gtggcgctgc
     2221 gcggctacgt caagaacgac gcgatggtcg gccgcatcca cgcgctcgag atcgtctggc
     2281 accgggcgct ggcgccgggc tacggctgga tcttcccgtg ccgcgacggc gtgttcaaca
     2341 tcggcgtggg catcatcgac agccacaacg cgcgccgagg cgagcggctg acgaagcgcg
     2401 aggtcaacct gcgccaggtg atgaacgact tccaggccgt gtacgaaccg gcgcgcgcgc
     2461 tgatgcaggg cggcaccgag ctcggcccga tgaagggcgc gccgctgcgc tgcacgctcg
     2521 aaggcgcacg ctacacgcgg cccggcctgc tggtgatcgg cgaggccgcg ggcagcacgt
     2581 attcgtttac cggcgagggc atcggcaagg ccttcgagac cgccatccac gcggccgagg
     2641 cgctgctggc gcacggcgcc gcgcccgcgg ccgacgccgc ggtgcgcgcc gactacgagg
     2701 cccggctgcg cgcgctgaag ccgcgcttcg atctctacca gcgcgccaac gtcgtgaacc
     2761 gccacccgtg gctggccgac ctggtgatct ggcgcgccca gcgcagcccg cgcctcgtgc
     2821 ggcgcatgag cggcgtgctc aacgagacca gcaacccggg ccacctgctg agcgtgaagg
     2881 gcttcacgcg cctgttcctc gaccgcgcct gaaccatgtg ccagctcctg ggcatgaacg
     2941 ccaacacgcc gaccgacgtg atgttcagct tcaccggctt cgccacgcgc gcgcaggagc
     3001 acaaggacgg tttcggcatc gcgttcttcg agggcgccgg cgtgcgcctg ctggtcgatg
     3061 cgcagagcgc gcgcagttcg ccggtggccg agatggtgcg gcgctttccg atccacagcc
     3121 gcaacatcgt cgcgcacatc cgcaaggcga cgcagggcca ggtgagcctg gcgaacacgc
     3181 acccgttcgt gcgcgagctg tggggccgct actgggtgtt cgcgcacaac ggcgacctga
     3241 aaggcttcgc gccgcgcctg cacggcgcgt ttcgcccggt cggcgagacc gacagcgagc
     3301 gcgcgttctg ctggctgatg caggagctcg cgaaggccca cgccagcgtg ccgacgacgc
     3361 ccgagctcac cgcgaccctg cgcgaactcg cgccgattcc cgccgcacac ggcacgttca
     3421 acttcatgct cagcaacggc caggcgctgt gggcacattg ctcgacgcag ctgcatcacc
     3481 tcgtgcgcca gcacccgttc gcgcaggcct cgctgcagga cgacgatgtg acggtggact
     3541 tctccaagca caccaccgag cacgaccgcg tcgccgtgat cgtcaccgag ccgctgacgc
     3601 gcaacgaggc ctgggtcgcg ttcgcgccgg gcg
//
LOCUS       DASRDG010000036         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_465942, whole genome shotgun sequence.
ACCESSION   DASRDG010000036 DASRDG010000000
VERSION     DASRDG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465942"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /gene="pyrG"
                     /locus_tag="VFA35_00525"
     CDS             complement(<1..433)
                     /gene="pyrG"
                     /locus_tag="VFA35_00525"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794470.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HZT54679.1"
                     /translation="MTKYVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFISTRMKRSNNFTTGKIYQSVLEKE
                     RRGDYLGKTVQVIPHVTNEIQEFVKRGANDVDVAIVEIGGTV"
     gene            complement(481..1308)
                     /locus_tag="VFA35_00530"
     CDS             complement(481..1308)
                     /locus_tag="VFA35_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT54680.1"
                     /translation="MPVPPRVIVFSHANGFPAGTYRLIFEAWRAAGYAVHAIEKYGHD
                     PRFPVTSNWPQLREQLIDFIEREVGGPAFLVGHSLGGYVSLMAASKRPDLASGLVMLD
                     SPVLAGWKARALQVAKAAGVGERFSPGHVSKRRRQQWDSADAAFAHFAAKPAFARWAP
                     GMLRDYIAAGTETHGAHRRLAFHRDVETAIYNTLPHHLARVLRAHPLQCEVGFVRGTD
                     SAEVRQVGMRATERLAKGKVAAIEGSHLFPFERPAETAAVVLQLIESMPRPEGTRRL"
     gene            complement(1308..2132)
                     /locus_tag="VFA35_00535"
     CDS             complement(1308..2132)
                     /locus_tag="VFA35_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HZT54681.1"
                     /translation="MTYCVGIKLDAGLVFMSDSRTNAGLDQISTYRKMMVYEKPGDRF
                     MVMLSAGNLSISQSVREILQVEKIEDRDGLPVTIWNAKSMFDAVRVLGAAVRRVHDQD
                     GAALERVGVDFNASMIFGGQIGSEAMRLFLVYSAGNFIEATRETCYFQVGESKYGKPV
                     LDRMITPTTPLDEAAKCALVSMDSTLKSNLSVGLPLDLLVYEAHKLQTDQIVCIDEHN
                     PYFQMIRGNWGLKLREVFESLEDPQWGAGPTAVPLRVPSQRYETMKKITHPDEKVV"
BASE COUNT          350 a          698 c          751 g          382 t
ORIGIN      
        1 cgaccgtgcc cccgatctcg acgattgcca cgtcgacatc gttcgcgccg cgcttgacga
       61 actcctggat ctcgttggtg acgtgcggaa tcacctgcac ggtcttgccg agatagtcgc
      121 cgcggcgctc cttctcgagc accgactggt agatcttgcc cgtggtgaag ttgttcgagc
      181 gcttcatgcg cgtgctgatg aagcgctcgt agtggccgag atcgaggtcg gtctcggcgc
      241 cgtcgtcggt gacgaacacc tcgccgtgct ggaacggcga catcgtgccc ggatcgacgt
      301 tgaggtacgg atcgagcttg atgagggtga ccttgaggcc gcgcgattcg aggatcgcgg
      361 cgagagacgc agaagcgatg cccttgccca gcgaggacac cacaccgccg gtgacaaaga
      421 cgtacttggt cattgtgtgc agcacttgtt cgacacaaat gctctggtgg taaagcgcga
      481 ttataggcgt cgcgtcccct cgggccgggg catggattcg atcagttgca acaccacggc
      541 ggcggtttcg gccgggcgtt cgaacgggaa caggtgggaa ccctcgatcg ccgcgacctt
      601 gcctttcgcg aggcgctcgg tcgcgcgcat gcccacctgc cgcacctcgg ccgagtcggt
      661 gccgcgcacg aaccccacct cgcattgcag cggatgcgcg cgcagcacgc gcgcgaggtg
      721 gtgcggcagc gtgttgtaga tcgcggtctc gacgtcgcgg tggaaggcca ggcgccggtg
      781 cgcgccgtgg gtttcggtgc cggcggcgat gtagtcgcgc agcatgcccg gtgcccagcg
      841 cgcgaacgcc ggcttggcgg cgaagtgcgc gaaggccgcg tcggcactgt cccattgctg
      901 gcggcggcgc ttcgagacgt ggccgggcga gaagcgctcg ccgacgccgg cggccttcgc
      961 gacctgcagc gcgcgcgcct tccagcccgc gagcaccggc gagtcgagca tcacgaggcc
     1021 gctggccaga tcggggcgtt tcgacgcagc catcaggctg acgtagccgc cgagcgaatg
     1081 gccgacgagg aaggccgggc cgccgacctc gcgctcgatg aaatcgatca gctgctcgcg
     1141 cagctgcggc cagttgctcg tgaccgggaa gcgcgggtcg tggccgtact tctcgatcgc
     1201 gtggaccgcg tagccggcgg cgcgccaggc ctcgaagatc agccgatagg tgccggcggg
     1261 gaagccgttc gcatgcgaaa agacaatgac gcgcggcggc accggcatca aaccaccttc
     1321 tcgtccgggt gcgtgatctt cttcatcgtc tcgtagcgct gcgacgggac gcgcagcggc
     1381 acggcggtcg gcccggcgcc ccattgcggg tcttcgagcg actcgaagac ctcgcgcagc
     1441 ttcaggcccc agttgccgcg gatcatctgg aagtacgggt tgtgctcgtc gatgcagacg
     1501 atctggtcgg tctgcaactt gtgcgcctcg tagaccagca gatccagcgg caggccgacg
     1561 gagaggttcg acttcagcgt cgagtccatc gagaccagcg cgcacttggc ggcctcgtcg
     1621 agcggcgtcg tcggcgtgat catgcggtcg agcaccggct tgccgtactt cgactcgccg
     1681 acctggaaat agcaggtctc gcgcgtcgcc tcgatgaagt tgccggccga gtagacgagg
     1741 aacagccgca tcgcctcgct gccgatctgg ccgccgaaga tcatcgacgc gttgaaatcg
     1801 acgccgacgc gttcgagcgc ggcgccgtcc tggtcgtgga cgcggcgcac cgcggcgccg
     1861 agcacgcgca ccgcgtcgaa catgctcttc gcgttccaga tcgtgacggg caagccgtcg
     1921 cggtcctcga tcttctcgac ctgcaggatc tcgcgcaccg actggctgat cgagaggttg
     1981 ccggccgaca gcatcaccat gaagcggtcg cccggcttct cgtacaccat catcttgcgg
     2041 taggtgctga tctggtcgag cccggcgttg gtgcgcgagt cggacatgaa caccaggccg
     2101 gcgtcgagct tgatgcctac acagtaggtc attgtgtttt tccttggaga gacttgagtt
     2161 cgtagagcgc ggcgagcgct t
//
LOCUS       DASRDG010000037         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_700780, whole genome shotgun sequence.
ACCESSION   DASRDG010000037 DASRDG010000000
VERSION     DASRDG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700780"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFA35_00540"
     CDS             complement(<1..398)
                     /locus_tag="VFA35_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZT54682.1"
                     /translation="MVDSVWPASLYKKHPSLRSDEREYCFMETNYLRNFMVVLESGSM
                     SEAARRLDLTPAAVAQQMRVLERDFGVPLLRRAGRTVSPTEGGHRLADRARVLLAELT
                     TMRTVVGDGEGALELTVGATNTMLNGPLPMV"
     gene            397..1260
                     /locus_tag="VFA35_00545"
     CDS             397..1260
                     /locus_tag="VFA35_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZT54683.1"
                     /translation="MRSKLFVPGSRPELFDKALRGEADALSFDLEDSVVPSRKADARH
                     ALQGLLSGPAARASGKTLIARVNPRGSEHFEADVRAVVRPGLALLNLPKVEATEDVLT
                     AAKALEAAERANGVSEPVGLLLNIESARGLRLAYALATAHPRVAGLQLGLGDLFEPLQ
                     IARHEPAALHTVMLQLRLAAGEAGVFAYDAAYARFQDVDGFRAEAGLARRLGYLGKSC
                     IHPSQVAHANEVFRPTDDEIAQARRVAAAADDAEARGVGAYTVDGEMVDAPFVLRARA
                     VLATARRMGLD"
     gene            1328..2308
                     /locus_tag="VFA35_00550"
     CDS             1328..2308
                     /locus_tag="VFA35_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZT54684.1"
                     /translation="MSTPSLSRRRACIAVAALCALGAPFAQAQSYPSKPITIVVPYAP
                     AGSNDNFARVLGKGLSAALKQPVIVDNKPGASGNMGSAFVAKAPADGYTLLVVSSSLV
                     TNAAIQTKLPFDAVKDLAPVAMIAKGPFIVAVNNDFPAKTPKELVSVLKAAPGKYAYA
                     SSGIASTNQFATEQLKALSGTFVLHVPYKGIGPAVTDLIGNQVQLLISSGPSIMPFVK
                     GGRMRAIAVTSAEPSPIAPELPPMASAVPGYNFEAWWGLLAPAGTPPEIVARLNSEVN
                     KVLASPEFKEFLLKEGALPAPMTPAQFAATIAADIPRWKKLAKQQNIVPE"
     gene            2305..>3388
                     /locus_tag="VFA35_00555"
     CDS             2305..>3388
                     /locus_tag="VFA35_00555"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZT54685.1"
                     /translation="MSTSAPHPSSLGPLAGVRVLDLSAYIAGPYGCTLLADQGAEVLK
                     VEPPGGDNLRHYPSTLAGESRAFVGVNRSKRGVVLDLKQPADRELLLCLVRDADVLVH
                     NFRPGVPERLGIAYAQLQPLNDRLIYCAVTGFGETGPLKDKAGYDQVLQTYSGMCDEQ
                     GQRGGPPEILYGSVVDYFASAMVASGVASALYEREKSGRGQQVGVSLLRAALTMQSAR
                     LVWGEDEPRDVSRDMRSGGITGLHPTREGYLYLSANTPHFWRALCEKTGLMALHDDPR
                     YDSVRKRAAHRDEIVPRLREALTARTALEWEAEFGESVPCAASRSVADMFDDPQVRAE
                     AMVTTLAHPTLGSYRGFTQPWVYSRTP"
BASE COUNT          517 a         1241 c         1144 g          486 t
ORIGIN      
        1 accatcggca gcgggccgtt gagcatggtg ttggtggccc cgacggtcag ctccagcgcg
       61 ccctcgccgt cgccgacgac cgtgcgcatc gtcgtgagtt cggcgagcag gactcgcgct
      121 cggtcggcca ggcggtgtcc gccttcggtc gggctgacgg tgcggccggc gcggcgcagc
      181 aggggcacgc cgaaatcgcg ctcgagaacg cgcatctgct gggccaccgc ggccggggtc
      241 aggtccagac gtcgcgccgc ctccgacatc gagcccgact cgagcaccac catgaagttg
      301 cggaggtagt tggtctccat gaagcaatat tctctttcat ctgatcgaag actcgggtgc
      361 tttttatata agcttgccgg ccacacactg tcgaccatgc gcagcaagct cttcgttccc
      421 gggtcacggc ccgaactgtt cgacaaggcc ctgcgcggcg aagccgatgc gctctccttc
      481 gatctggaag actcggtcgt tccgagccgc aaggccgatg cacgccacgc gctgcagggc
      541 ctgctgtccg gcccggccgc ccgcgcctcg ggcaagacgc tgatcgcgcg ggtcaacccg
      601 cgcggctcgg agcacttcga ggccgatgtg cgagccgtgg tccgccccgg gctcgcgttg
      661 ctgaacctgc ccaaggtcga agcgaccgag gacgtgctta cagctgccaa agcgctcgaa
      721 gcggccgaac gcgccaacgg cgtcagcgag ccggtcggcc tgctcctcaa catcgagagc
      781 gcgcgagggc tgcgcctcgc gtacgcgctc gcgacggcgc atccgcgcgt ggccgggctg
      841 cagctcggcc tcggcgacct gttcgagccg ctgcagatcg cccgccacga accggcggcg
      901 ctgcacaccg tgatgctgca gctgcgcctc gcggccggcg aggccggtgt attcgcctac
      961 gacgccgcgt acgcccgctt tcaggacgtc gacgggttcc gcgccgaggc cggcctggca
     1021 cggcgcctcg gctacctcgg caagagctgc atccacccga gccaggtggc gcacgccaac
     1081 gaagtgttcc gccccaccga cgacgagatc gcgcaggcgc gccgcgtggc cgccgcggcc
     1141 gacgatgccg aggcccgcgg cgtcggcgcc tacaccgtcg acggcgagat ggtcgacgcg
     1201 ccgttcgtgc tgcgcgcgcg cgcggtgctc gccaccgcgc ggcgaatggg gctcgactga
     1261 agactcgccc cccgcgcacg ccgcccctcg ccacgaccga accgcattcc gcaggagata
     1321 cgaacccatg tccacgccat ccctttcccg acgccgcgcc tgcatcgccg tggccgcgct
     1381 gtgcgccctc ggtgcaccgt tcgcgcaagc gcagagctat ccgagcaagc cgatcacgat
     1441 cgtcgttccc tacgcgcccg ccggctcgaa cgacaacttc gcgcgcgtgc tgggcaaggg
     1501 gctgagcgcc gcgctcaaac agccggtgat cgtcgacaac aagccgggcg cgagcggcaa
     1561 catgggttcg gccttcgtgg ccaaggcgcc ggccgacggc tatacgctcc tggtggtgtc
     1621 gtcgagcctg gtcaccaacg ccgcgatcca gaccaagctg ccgttcgacg ccgtgaagga
     1681 cctggcgccg gtagcgatga tcgccaaggg cccgttcatc gtcgccgtga acaacgactt
     1741 cccggcgaag acgccgaagg agctggtgtc ggtgctgaag gctgcgccgg gcaagtacgc
     1801 gtacgccagc tccggcatcg ccagcacgaa ccagttcgcc accgagcagc tcaaggcgct
     1861 gagcggcacc ttcgtgctgc acgtacccta caagggcatc ggcccggccg tcaccgacct
     1921 gatcggcaac caggtgcagc tgctgatctc cagcgggcca tcgatcatgc cgttcgtcaa
     1981 gggcgggcgc atgcgcgcga tcgccgttac cagcgccgag ccgagcccga tcgcgccgga
     2041 gctgccgccg atggccagcg ccgtgccggg ctacaacttc gaggcctggt ggggcctgct
     2101 cgcgccggcc ggcacgccgc cggagatcgt cgcgcgcctc aactccgagg ttaacaaggt
     2161 gcttgcctcg cccgagttca aggagttcct gctgaaggaa ggcgccttgc ccgccccgat
     2221 gacgccggcg cagttcgcgg cgaccattgc cgccgacatc ccgcgctgga agaagctcgc
     2281 gaagcagcag aacatcgtcc ccgaatgagc acctccgccc cccacccgtc ttcgctcggg
     2341 ccgctggccg gcgtgcgcgt gctcgacctg agcgcctaca ttgccgggcc ctacggctgc
     2401 accctgctcg ccgaccaggg cgccgaggtc ctcaaggtcg agccgcccgg tggcgacaac
     2461 ctgcgccact acccgtcgac gctggccggc gagagccggg ccttcgtcgg cgtcaaccgc
     2521 agcaagcgcg gcgtcgtgct cgacctcaag cagcccgccg accgcgagct gcttttgtgc
     2581 ctggtgcgcg acgccgacgt gctggtgcac aactttcgcc ccggcgtgcc cgagcggctc
     2641 ggcatcgcgt atgcccagct gcagccgctc aacgaccggc tgatctattg cgccgtcacc
     2701 ggcttcggcg agaccgggcc cctcaaggac aaggccggct acgaccaggt gctgcagacc
     2761 tacagcggca tgtgcgacga gcagggccag cggggcgggc cgcccgagat cctctacggc
     2821 tcggtggtcg actacttcgc ctcggcgatg gtggcctcgg gagtcgcctc ggcgctgtac
     2881 gagcgcgaga agagcgggcg cggccagcag gtcggcgtgt cgcttctgcg cgccgcgctg
     2941 accatgcagt cggcgcgcct ggtgtggggc gaggacgagc cgcgcgatgt cagtcgcgac
     3001 atgcgctcgg gcggcatcac cggcctgcac ccgacgcgcg agggctacct gtacctgtcg
     3061 gccaatacgc cgcacttctg gcgcgccttg tgcgagaaga cagggctgat ggccctgcac
     3121 gacgacccgc gctatgacag cgtgaggaag cgcgccgcgc atcgcgacga gatcgtgccg
     3181 cgcctgcgcg aggcgctcac ggcgcgcacg gccctcgagt gggaggccga gttcggcgag
     3241 agcgttccct gcgccgcgtc gcgcagcgtg gccgacatgt tcgacgaccc gcaggtgcgg
     3301 gccgaggcga tggtcaccac gctcgcccat ccgacgctcg gcagctaccg cggcttcaca
     3361 cagccctggg tctacagccg cacgccag
//
LOCUS       DASRDG010000038         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_811311, whole genome shotgun sequence.
ACCESSION   DASRDG010000038 DASRDG010000000
VERSION     DASRDG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811311"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..934)
                     /locus_tag="VFA35_00560"
     CDS             complement(14..934)
                     /locus_tag="VFA35_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HZT54686.1"
                     /translation="MNAGFGFTGAGPLLGTAALLAALLLLGRWLERRPVRAALAIART
                     LPPLAVGAAALATDAQPAGTRMLVITAALFVAMKCLVLAAARADGEAPLARRDWWSFA
                     LLWPGMAPGAFAVRGRSDRAAARRFAARGLHNALAGAALVLLARALGSIWLAPMLMVG
                     LSLCVHFGLFTLLAALHRAAGRRVRPTFDAPHRSRSLGDFWAHRWNRGFAEMTALLVQ
                     RPLAARFGRKPALLLSFLASGILHEVAISLPVRAGYGLPTLYFVLQGALVAWLPKQHG
                     RAVALLAVLLPLPLVFHPPFVRGVIAPLLR"
     gene            complement(942..1343)
                     /locus_tag="VFA35_00565"
     CDS             complement(942..1343)
                     /locus_tag="VFA35_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54687.1"
                     /translation="MRALLVVTGLGQIALAIASLWLPRVLGWRAQTAALQPLTRRVFW
                     VYAAYILGTNLCLGSLSAFAPALLLDRSPLARTVAAYATAYWGVRLLIQFVWFRGVAP
                     QGTFYRTADLLVTLGFLLWTCCYGAILLDAW"
     gene            1437..>2098
                     /locus_tag="VFA35_00570"
     CDS             1437..>2098
                     /locus_tag="VFA35_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54688.1"
                     /translation="MSVSGPSSVRYALLLACGYVAVTAVYIWLSSLLAAGASHSVSEM
                     LTIETRKGLSFVGVTGALLFFGALWVTRRLQRDAAILSRQERALVANEGRVVAGLMAA
                     SVAHDANNVLAVVLGELDELRRSLGRDDGAVRRLETAAQKLVALNRRLLDTAREGTPG
                     DRSLVDLAAAAHEVVATSRAHAVVRGCRVSVLAEGSHSVLAPVVVVQRIIGNLLLNAA
                     EAT"
BASE COUNT          302 a          765 c          806 g          225 t
ORIGIN      
        1 gcgcgggccg tgctcatctc agcaatggcg cgatcacgcc gcgcacgaac ggcggatgga
       61 acacgagcgg cagcggcagc agcaccgcga gcaaggcgac agcgcggccg tgctgcttcg
      121 gcagccatgc gaccagcgcg ccctgcagca cgaagtagag cgtcggcagg ccgtagcccg
      181 cgcgcaccgg cagcgagatc gcgacttcgt gcaggatccc cgaggcgagg aacgacagca
      241 gcagcgccgg cttccggccg aagcgggctg ccagcggccg ctgcacgagc agcgccgtca
      301 tctcggcgaa gccgcggttc cagcggtgcg cccagaagtc gccgagcgag cggctgcggt
      361 gcggcgcgtc gaacgtcggc cgcacgcggc ggccggcggc gcggtgcagc gccgcgagca
      421 gcgtgaacag gccgaagtgc acgcagaggc tcagcccgac catcagcatc ggcgcgagcc
      481 agatcgagcc gagcgcgcgc gcgagcagca cgagggccgc gccggccagc gcgttgtgca
      541 gcccccgcgc ggcgaagcgg cgcgccgcgg cgcggtcgct gcggccgcgc acggcgaacg
      601 cgccgggcgc catgccgggc cacagcagcg cgaacgacca ccagtcgcgg cgcgcgagcg
      661 gcgcttcgcc gtcggcgcgc gcggcggcga gcaccaggca cttcatcgcc acgaacagcg
      721 ccgccgtgat cacgagcatg cgcgtgccgg ccggctgcgc gtccgtcgcc aacgccgccg
      781 cgcccaccgc gagcggcggc agcgtgcgcg cgatcgccag cgccgcgcgc accggccggc
      841 gttcgagcca gcggccgagc agcagcagcg ccgcgagcag cgcagcggtg ccgagcagcg
      901 gcccggcccc cgtgaagccg aagccggcgt tcaccggtcg ttcaccacgc gtcgagcagg
      961 atcgcgccgt agcagcaggt ccagagcagg aagccgagcg tcaccagcag gtcggcggtg
     1021 cggtagaacg tgccctgcgg cgccacgccg cggaaccaga cgaactggat cagcagccgg
     1081 acgccccagt aggcagtcgc gtaggcggcg accgtgcgcg cgagcggact gcggtcgagc
     1141 agcagcgccg gcgcgaacgc gctgagcgag ccgaggcaga ggttggtgcc gaggatgtac
     1201 gcggcgtaga cccagaagac ccgccgcgtc aggggctgga gcgccgcggt ctgcgcgcgc
     1261 cagccgagca cgcgcggcag ccacaagctc gcgatcgcca gcgcgatctg gccgaggccg
     1321 gtgacgacga gcagcgcgcg catccgggca gcatgcccgc cggccgcccc gctgtcatgg
     1381 ctgccgcgcg ttgaccgcgg cgggcgcgcg cagcatgctc ggctcggagg tgcgcgatgt
     1441 cggtctccgg gccgagcagt gtccgctacg cgctgttgct cgcctgcggc tacgtggccg
     1501 tgacggccgt ctacatctgg ttgtcgtcgc tgctcgcggc cggtgcgtcg cactcggtgt
     1561 ccgagatgct gaccatcgag acgcgcaagg ggctgtcgtt cgtcggcgtc accggcgccc
     1621 tgctgttctt cggcgcgctg tgggtcacgc ggcgcctgca gcgcgacgcg gcgatcctgt
     1681 cgcgtcagga gcgcgcgctg gtcgccaacg aaggccgcgt cgtcgccggg ctgatggccg
     1741 cgtcggtggc gcacgacgcc aacaacgtgc tggccgtcgt gctcggcgag ctcgacgaac
     1801 tgcgccggtc gctgggccgc gacgacggcg cggtgcgccg gctcgagacg gcggcgcaga
     1861 aactcgtggc gctcaaccgg cggctgctcg acacggcgcg cgaagggacc cccggcgacc
     1921 gttcgctggt cgacctcgcg gccgccgcgc acgaggtcgt cgcgacctcg cgcgcgcacg
     1981 cggtggtgcg cggctgccgc gtctcggtgc tcgccgaggg cagccattcg gtgctcgcgc
     2041 cggtggtagt ggtgcagcgg atcatcggca acctgctgct caacgccgcc gaggccac
//
LOCUS       DASRDG010000039         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_929929, whole genome shotgun sequence.
ACCESSION   DASRDG010000039 DASRDG010000000
VERSION     DASRDG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929929"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /gene="mutL"
                     /locus_tag="VFA35_00575"
     CDS             complement(<1..1007)
                     /gene="mutL"
                     /locus_tag="VFA35_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZT54689.1"
                     /translation="MNAVLLPSIRRPIRELPDELVSQIAAGEVVERPASVVRELVDNA
                     LDAGATEIAVKLVGGGIRAIVVEDDGAGIPADELPLALRRHATSKIASLAELEGVATM
                     GFRGEALAAIASIAEVGIASRTADAPHARRLDARSGELAPAARGVGTSVEVRELFFAT
                     PARRKFLKTEATEFAHCLEAVRRHALARPEVGFTVWHDGKLSAQWRRASAEQRLVDVL
                     GHEFVEHSRALAIASGVLRITGRSGTPEAARARADQQYVYVNGRFVRDKLIAHGVRSA
                     YEDVLHGARQPAYVLFIEIAPDRVDVNVHPTKVEVRFRDAREVHQAVRRAVETALAPP
                     RA"
     gene            1056..1703
                     /locus_tag="VFA35_00580"
     CDS             1056..1703
                     /locus_tag="VFA35_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZT54690.1"
                     /translation="MEILHFVLDFILHVDVHLREFVQAYGSWVYALLFLIIFVETGVV
                     VMPFLPGDSLLFVVGALCGAGLMSLPLVLVLLIAAAILGNQSNYTIGRHFGPRVFQWE
                     NSKFFNKNAFDQAHAFYEKYGGATIVAARFMPFLRTFAPFVAGVAEMTRSKFTLFDVV
                     GAVLWVGGVTTAGYLFGEIPWVKSHLDKIIWGAILLPGLLVLFGAWRARRQKALS"
     gene            1750..2040
                     /locus_tag="VFA35_00585"
     CDS             1750..2040
                     /locus_tag="VFA35_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54691.1"
                     /translation="MNGPTAYTPVRGRGRMGHQQVLVGDKPVGEIWREAVGVPHSTAR
                     NPMQWRWFARAANDETVLGRGTRLALIRGPGFTSKNKATEALLARVADLKRR"
     gene            complement(2065..3006)
                     /locus_tag="VFA35_00590"
     CDS             complement(2065..3006)
                     /locus_tag="VFA35_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT54692.1"
                     /translation="MLHPQTRALLDLIEARQIPPTHTLSVADARAFYRDRRAVTQPAP
                     PAVAQVRELQASGPHGSIPLRLYRPLGSAESAVLPVLVYYHGGGWTIGDLDTHDVLCR
                     ELANGAGCAVVAVDYRMGPEHRFPAAVDDCIAATRWVRAHAAELRLDATLLAVGGDSA
                     GGNLAAVVSIAARDSGDLPIVFQLLIYPATDAHRSHPSHTENGQGYLLTKETMDYFSD
                     HYLPDRSQYDDWRVSPLLREDLAKLPPALVLTAGYDPLRDEGEAYAERLTAAGNRASY
                     VCFERQIHGFITMGKVLDEANTAVALCAAELRRAFGA"
BASE COUNT          413 a         1074 c         1109 g          474 t
ORIGIN      
        1 gcgcgcgggg gggccagcgc ggtctccacc gcgcgccgca ccgcctggtg cacctcgcgc
       61 gcatcgcgaa accgcacctc gaccttggtc gggtgcacgt tcacgtcgac ccggtcgggc
      121 gcgatctcga tgaacagcac gtaggccggc tggcgcgcgc cgtgcagcac gtcttcgtag
      181 gccgagcgca cgccgtgcgc gatcagcttg tcgcgcacga agcggccgtt gacgtacacg
      241 tactgctggt cggcgcgcgc acgcgccgcc tcgggcgtgc cgctgcggcc ggtgatgcgc
      301 agcacgcccg aggcgatcgc gagcgcgcgg ctgtgctcga cgaactcgtg gccgagcacg
      361 tcgaccaggc gctgctcggc gctggcgcgc cgccactgcg cgctcagctt gccgtcgtgc
      421 cagaccgtga agccgacctc gggccgcgcc agcgcgtggc ggcgcaccgc ctcgaggcag
      481 tgcgcgaact cggtcgcctc ggtcttcagg aacttgcgcc gtgccggcgt cgcgaagaac
      541 agctcgcgca cctcgacgct ggtgccgacg ccgcgtgcgg ccggcgcgag ctcgcccgag
      601 cgcgcatcga gccgccgcgc gtgcggcgca tcggcggtgc ggctcgcgat gccgacctcg
      661 gcgatcgagg cgatcgccgc cagtgcctcg ccgcgaaagc ccatcgtcgc cacgccttcg
      721 agctcggcca gcgacgcgat cttgctggtc gcgtggcggc gcagcgcgag cggcagctcg
      781 tcggccggga tgcccgcgcc gtcgtcctcg acgacgatcg cgcggatgcc gccgccgacc
      841 agcttgaccg cgatctcggt cgcgccggcg tcgagcgcgt tgtcgaccag ctcgcgcacc
      901 accgaggccg ggcgctcgac gacctcgccg gcggcgatct ggctgacgag ttcgtcgggc
      961 agttcgcgga tcgggcggcg gatcgatggc aggagcacgg cgttcatcgg cgcattgtag
     1021 aaacccgcgt cgcgcgacca ccataatcgc gccgcatgga aatccttcac tttgttctcg
     1081 acttcatcct gcacgtcgac gtgcacctgc gcgagttcgt gcaggcctac gggagctggg
     1141 tctatgcgct gctgttcctg atcatcttcg tcgagaccgg cgtggtcgtg atgcccttcc
     1201 tgccgggcga ttcgctgctc ttcgtcgtcg gcgcgctgtg cggcgccggc ctgatgagcc
     1261 tgccgctggt gctggtgctg ctgatcgccg cggcgatcct cggcaaccag agcaactaca
     1321 cgatcggccg gcacttcggc ccgcgcgtgt tccagtggga gaactcgaag ttcttcaaca
     1381 agaacgcgtt cgaccaggcg catgcgttct acgagaagta cggcggcgcg accatcgtgg
     1441 ccgcgcgctt catgcccttc ttgcgcacct tcgcgccctt cgttgccggc gtggccgaga
     1501 tgacgcgcag caagttcacg ctgttcgacg tggtcggcgc cgtgctctgg gtcggcggcg
     1561 tcacgaccgc gggctacctg ttcggcgaga tcccgtgggt caagtcccac ctcgacaaga
     1621 tcatctgggg cgcgattctg ctgccggggc tgctggtgct gttcggcgcg tggcgggcgc
     1681 gacgccagaa ggccttgtca tagactacgc ggcggcgcga ctgacggtgc tctcctgcag
     1741 aatcaaaaca tgaacgggcc cacagcctac acccccgtgc gcggccgcgg ccgcatgggg
     1801 catcagcaag tgctggtcgg cgacaagccg gtcggcgaga tctggcgcga ggccgtgggc
     1861 gtgccccaca gcaccgcgcg caacccgatg cagtggcgct ggttcgcgcg cgccgccaac
     1921 gacgagaccg tgctcggtcg cggcacccgg ctcgcgttga tccgggggcc ggggttcacg
     1981 agcaagaaca aggcgaccga ggcgctgctc gcgcgggtcg cggacctgaa gcgcaggtag
     2041 gtctcgccta cccgcgcgtc gcgttcacgc gccgaacgcg cgccgcagct cggcggcgca
     2101 caacgcgact gcggtgttcg cttcgtcgag caccttgccc atcgtgatga agccgtggat
     2161 ctggcgctcg aagcagacat agctcgcgcg gttgccggct gcggtgagcc gctcggcgta
     2221 ggcctcgcct tcgtcgcgca gcgggtcgta gccggcggtc agcaccagcg ccggcgggag
     2281 cttcgcgagg tcttcgcgca gcagcggcga gacgcgccag tcgtcgtact gcgagcggtc
     2341 gggcaggtag tggtcgctga agtagtccat cgtctccttc gtcagcaggt agccctggcc
     2401 gttctcggtg tgcgacgggt ggctgcggtg cgcgtcggtc gcggggtaga tcagcagctg
     2461 gaacacgatc ggcagatcgc cgctgtcgcg cgccgcgatc gagaccaccg ccgcgaggtt
     2521 gccgccggcg ctgtcgccgc cgaccgccag aagcgtcgca tcgagccgca gctcggcggc
     2581 gtgcgcgcgc acccagcggg ttgcggcgat gcagtcgtcg accgctgcgg ggaagcggtg
     2641 ctccggcccc atccggtagt cgaccgcgac gacggcgcag ccggcgccgt tcgcgagctc
     2701 gcggcacagc acgtcgtggg tgtcgaggtc gccgatggtc cagccgcccc cgtggtagta
     2761 gacgagcacc ggcagcacgg cgctctcggc cgagccgagc ggccggtaca ggcgcagcgg
     2821 aatcgaaccg tgcgggccgc tcgcctgcag ctcgcgcacc tgcgccaccg ccggcggcgc
     2881 gggctgcgtg accgcgcgcc ggtcgcggta gaaggcgcgc gcgtcggcga cgctgagcgt
     2941 gtgggtcggc gggatctggc gcgcctcgat caggtcgagc aacgcgcggg tctgggggtg
     3001 cagcatgggg ctccttcgta cctcgtatcg acccgcaatg atggcccaga agaccatagc
     3061 gttgccttct
//
LOCUS       DASRDG010000040         6604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_930491, whole genome shotgun sequence.
ACCESSION   DASRDG010000040 DASRDG010000000
VERSION     DASRDG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930491"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFA35_00595"
     CDS             <1..591
                     /locus_tag="VFA35_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54693.1"
                     /translation="GPRVADFGDHPASPDARFVANWVAHSRDNAHLPFVILDKRDARV
                     FVFDAGAMLVDSSPVLLGAAAGDDSVIGIGKRPIKDVRPQERTTPAGRFVSRPGRNAG
                     GEDVVWVDYDAAVSMHRVRLVDPAERRLERLASADPAQRRISYGCINVPVAFFDAVIK
                     PVLGAQPAVVYVLPETRQLREVFAGAYPVEPTARRM"
     gene            738..1391
                     /locus_tag="VFA35_00600"
     CDS             738..1391
                     /locus_tag="VFA35_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54694.1"
                     /translation="MACRGEKLRAAAAALLLYALASWPALAAPDFGAEHASDDARYAA
                     ARVLATADHQQRPFAIVDKRDARIYVFEASGQLAGASSALLGLAPGDTAVADIAQRLP
                     ASLAPHERSTPSGRFESQPGHNDKGEAIVWVDYAAALAIHRLRPAPADERRAERLASA
                     TPDDNRISLGCIVVPVAFYEAVVEPALGRQRGVVYVLPETRPVQALFGADPLQLAKR"
     gene            1407..2708
                     /locus_tag="VFA35_00605"
     CDS             1407..2708
                     /locus_tag="VFA35_00605"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HZT54695.1"
                     /translation="MRRARRVHGAGRMRVLVLGSGVIGVTCAYELARAGHEVTVVDRQ
                     PAAALETSYGNAGEVSPGYSAPWAGPGVPVKAIKWLLMRHRPLVIRPHIDLNLLRWGV
                     AMLRNCTAARYELNKGRMVRLAEYSRDCLKELRAQTGIRYDARMQGTLQLFRTPAQLD
                     GTAADIAILKRYGVGFELLDRAGCIRHEPALASVDGKFVGGLRLPGDETGDCFKFTQG
                     LAALALAQGVRFRYGTQIRKLVRVGQQIDGVVTDAGKLQADAYLLALGSYAPLLLKNV
                     GIRIPVYPVKGYSITVPVTDAAGAPESTVMDESHKVGVTRLGDRIRVGGTAELAGYTL
                     KLHDARRHTLEHVVTDLFPRGGDVGRAEFWCGLRPMTPDGTPVVGGTRLPNLYLATGH
                     GTLGWTMAAGTGRVMADLISGRQPGIDMDGLTVERYPNAYA"
     gene            complement(2728..4650)
                     /locus_tag="VFA35_00610"
     CDS             complement(2728..4650)
                     /locus_tag="VFA35_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HZT54696.1"
                     /translation="MTFLLPTLGRVTSAAVLGLLIHTTATAQAPRPTDTLYPGTLTLQ
                     VDATDLDRRIFRIRETVPVAAGPLRLYHPQWLPGNHGPTGRIGDLAGLQISAGGAALA
                     WRRDPLDPYAFTLDVPAGAAVLDIEFQHLSPLNRESGRVVMTPEMLNVQWNAMLLYPA
                     GHYDSRITIRPSLRLPEGWRFGSALEAAARNGATTEFKPVSVETLIDSPVFAGRHMVR
                     VDLDPDAAAQGRAPVSLDVMGDTPDEIRITPEQLAAHRALVTQADRLFGARHFAHYDF
                     LFAISAEFGRIGLEHHQSSENAVTPGYFSDSKRASVGRDLLPHEYVHSWNGKFRRPAD
                     LWTPNTNTPMQDSLLWLYEGQTQFWGYVLAARSGLIPAAEMRDTLANAAAWLSAQKGR
                     AWRNLQDTANQAVMGERRERRDWRDWQRGSDYYEEMVLVWLEADMLIRDASGGQRSLD
                     DFARGFFGVDPGRVVPLTYRFEDVVAALDAVQPHDWARFLRERLDKHTDAGLLAGLER
                     AGWRLGWSEEPNSATKGWMAQRRFDDFSYSLGIEVGKDGALSAVRWGSPAFEAGLSTS
                     VQLVAVNGLAYKAERLKAAITGAKSGAAPIQLLVKDGERYRTVSIAYTGGLRYPQLER
                     IDGVPDRLTTLLAPRP"
     gene            complement(4724..4909)
                     /locus_tag="VFA35_00615"
     CDS             complement(4724..4909)
                     /locus_tag="VFA35_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54697.1"
                     /translation="MASDVDWVADVRRWYFSGRQQPAPTAPARGDAAEVGYEAADPAL
                     QSPHWPDAPRAGITDPA"
     gene            5179..>6604
                     /locus_tag="VFA35_00620"
     CDS             5179..>6604
                     /locus_tag="VFA35_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54698.1"
                     /translation="MPMKRIPGHARRAPWQVLRDLGEVSPWREVIDEYGGDSGRFHER
                     HYSEFVTFLPYVQRFLYGEGRKARGAEGRDADAPMRVFRREDIAHVRVVARPGDAPVT
                     LEVIHVDLYFFYDVDIVLLNLEIGANDLTLDQAQEQLYRFGRGYPAGWDVSGHALHTM
                     ASVEWLDAAGAALARSDAQQRELFMAHVAEHRAPRTAAHWAFMLKPLVADHSEEPGEL
                     RYRQIEYYRMPLMGYLALDDPRALTRDDFIRLGLVTGAGGSDNGSLIDDTDPRATDDG
                     SALQGRRDGSALPYAADHLADFEQRYCYDRFWANAGAAPNTRYLCSGHALIVIGDARS
                     TFYGCRDRGVLAQFRHQHFLLFLIAHFQKAALLMLSDRLVVALSRLDIQDVDTIRRFK
                     RSIRQMLEIFLRFTHRYWFHEVADQAQARALFRLCAQHLDLDGLYREVKDRIYDMNEY
                     LDSDSLRRQANTVLRLTVVTTFGLI"
BASE COUNT          920 a         2379 c         2324 g          981 t
ORIGIN      
        1 gggcctcgcg tggccgactt cggcgatcac cccgcatcgc ccgacgcccg cttcgtcgcc
       61 aactgggtgg cgcactcgcg cgacaacgcg catctgccgt tcgtgattct cgacaagcgc
      121 gacgcccgcg tcttcgtgtt cgacgccggc gccatgctgg tcgattcgag cccggtgctg
      181 ctcggcgcgg cggcgggcga cgactcggtc atcggcatcg gcaagcgtcc gatcaaggat
      241 gttcgtccgc aggagcggac cacgcccgcc ggccgcttcg tcagccggcc ggggcgcaat
      301 gcggggggcg aagacgtggt gtgggtggac tacgacgccg cggtgtcgat gcaccgggtg
      361 cgcctggtcg acccggccga acgccggctc gagcgcctgg cctcggccga tccggcgcag
      421 cggcgcatct cctacggctg catcaacgtg ccggtcgcgt tcttcgacgc tgtgatcaag
      481 ccggtgctgg gtgcgcaacc ggcggtcgtc tacgtgctgc ccgagacccg ccagttgcgc
      541 gaagtctttg ccggcgccta cccggtggag cccacggcgc gacggatgta acgaattccc
      601 tctgcgtgtg accgggtttt cgccgcacac gcgccccttg acgccagaat ccgctccggc
      661 gtgccgcttc tcggccgccc ggtgggtgca gtacgtcagc ctcgccgctg acgactccca
      721 ccgaaggatg gtttccgatg gcttgtcgag gcgaaaagct gcgcgccgcc gcagccgcgc
      781 tgctgctgta tgcgctcgca tcctggcccg ccctcgcggc gcccgacttc ggcgccgagc
      841 acgcctccga tgacgcgcgc tacgctgcgg cccgtgtcct ggccaccgcc gaccaccagc
      901 aacggccatt cgcgatcgtc gacaagcgcg acgcgcgcat ctacgtcttc gaggcgagcg
      961 gccaactcgc cggcgcatcg agtgcgctgc tcggcctggc gcctggcgat accgcagtcg
     1021 ccgacatcgc ccagcgcctt cccgccagcc tcgcgccgca cgagcgcagc acgccatcgg
     1081 ggcgcttcga atcgcagccc ggccacaacg acaagggcga ggcgatcgtc tgggtcgact
     1141 acgccgcggc gctcgcgatc caccggctgc gcccggcgcc cgccgacgag cggcgtgccg
     1201 agcggctcgc ctccgccacg cccgacgaca accgcatctc gctcggctgc atcgtcgtgc
     1261 cggtggcgtt ctacgaggcg gtcgtcgagc cggcgctcgg gcgccagcgt ggcgtggtgt
     1321 atgtgctgcc cgagacgcgc ccggtgcagg cgttgttcgg cgccgacccg ctccaactcg
     1381 ccaaacgctg agcccggccc cgtacactgc gccgggcacg aagggtgcat ggagcaggac
     1441 gcatgagggt gctggttctc ggcagcggcg tgatcggggt gacctgcgcc tacgagctcg
     1501 cgcgcgccgg tcacgaagtc acggtggtcg accgccagcc ggccgcggcg ctcgaaacca
     1561 gctacggcaa tgccggcgag gtctcgcccg gctactcggc gccgtgggcc gggcccggcg
     1621 tgccggtcaa ggcgatcaag tggctgctga tgcgccaccg gccgctggtg atccgcccgc
     1681 acatcgacct gaacctgctg cgctggggcg tggcgatgct gcgcaactgc accgccgcgc
     1741 gctacgagct gaacaagggc cgcatggtgc ggctggccga gtacagccgt gactgcctga
     1801 aggaactgcg cgctcagacc ggtatccgct acgacgcgcg catgcagggc acgctacagc
     1861 tctttcgcac ccccgcgcag ctcgatggca cggccgccga catcgccatc ctgaagcgct
     1921 acggcgtcgg cttcgagctg ctcgaccgcg cgggctgcat ccgccacgag ccggcgctcg
     1981 cgagcgtcga cggcaagttc gtcggcggcc tgcgtttgcc cggcgacgaa accggcgact
     2041 gcttcaagtt cacgcagggc ctcgcggcgc tggcgctggc gcagggcgtg cggttccgct
     2101 acggcacgca gatcagaaag ctcgtgcgcg tcggccagca gatcgacggg gtcgtcaccg
     2161 acgcgggcaa gctccaggcc gatgcctacc tgctcgcgct cggcagctac gcgccgctgc
     2221 tgctgaagaa cgtcggcatc cgcatcccgg tctatcccgt caagggctac tcgatcacgg
     2281 tgccggtcac cgatgcggcc ggcgcacccg aatcgacggt gatggacgag agccacaagg
     2341 tcggggtgac ccggctcggc gaccgcatcc gcgtcggcgg caccgccgag ctggccggct
     2401 acacgctgaa actgcacgat gcgcgccgcc acacgctcga gcatgtcgtg accgacctgt
     2461 tcccgcgcgg cggcgatgtc gggcgcgccg agttctggtg cggcctgcgc ccgatgacgc
     2521 ccgacggcac gccggtcgtc ggcggcacgc ggctgccgaa cctgtacctc gccaccggcc
     2581 acggcacgct gggctggacc atggcggcag gcaccggccg cgtcatggcc gacctgatct
     2641 ccgggcgcca acccggcatc gacatggacg gcctgaccgt cgagcgctac ccgaacgcct
     2701 acgcctgagc gcgcgggtcg cccaggttca aggccggggc gcgagcagcg tcgtcagccg
     2761 gtcggggacg ccgtcgatgc gctcgagctg cgggtagcgc aggccgccgg tgtaggcgat
     2821 cgacacggtc cggtagcgct cgccgtcctt caccagcagc tggatcggcg cagcgcccga
     2881 cttcgcgccg gtgatcgcgg ccttcagacg ctcggccttg taggccagcc cgttgacggc
     2941 gaccagctgc accgaggtgc ttagcccggc ctcgaacgcc gggctgcccc agcgcacggc
     3001 gctgagcgcg ccgtccttgc cgacctcgat gccgagcgag tacgaaaagt cgtcgaagcg
     3061 gcgctgcgcc atccagccct tggtcgcgct gttcggctcc tcgctccagc ccaggcgcca
     3121 gccggcgcgc tccaggccgg ccagcagccc ggcgtcggtg tgcttgtcga ggcgctcgcg
     3181 cagaaagcgc gcccagtcgt gcggctgcac ggcgtcgagc gcggcgacca cgtcctcgaa
     3241 ccggtaggtc aagggcacga cgcgcccggg gtcgacgccg aagaacccgc gcgcgaagtc
     3301 gtcgagcgag cgctggccgc ccgacgcgtc gcggatcagc atgtcggctt cgagccagac
     3361 cagcaccatc tcttcgtagt agtcgctgcc gcgctgccag tcgcgccagt cgcggcgttc
     3421 gcggcgctcg cccatcacgg cctggttggc ggtgtcctgc aggttgcgcc aggcgcggcc
     3481 tttctgtgcc gacagccagg cggccgcgtt cgcgagcgtg tcacgcatct cggcggccgg
     3541 tatcaggccc gaacgggcgg ccagcacgta gccccagaac tgcgtctggc cctcgtacag
     3601 ccacagcagc gagtcctgca tcggcgtgtt ggtgttcggc gtccacaggt cggccgggcg
     3661 gcggaacttg ccgttccacg agtgcacgta ctcgtgtggc agcaggtcgc ggcccaccga
     3721 cgcgcgcttc gagtcgctga agtagcccgg cgtcacggcg ttctcgctcg actggtggtg
     3781 ctccaggccg atgcggccga actcggcgct gatcgcgaac aggaagtcgt agtgcgcgaa
     3841 gtggcgcgcg ccgaagagcc gatcggcctg cgtcaccagc gcgcggtgcg cggcgagctg
     3901 ctcgggcgtg atcctgattt cgtccggcgt gtcgcccatc acgtccaggg acaccggcgc
     3961 gcggccctgc gccgcggcgt cggggtcgag gtcgacgcgc accatgtgcc ggccggcaaa
     4021 caccggcgag tcgatcaggg tctcgacact gaccggcttg aactcggtcg tcgcgccgtt
     4081 gcgcgccgcg gcctcgagcg cgctgccgaa gcgccagccc tcgggcaggc gcaggctcgg
     4141 cctgatcgtg atgcggctgt cgtagtgccc ggccgggtac agcagcattg cgttccactg
     4201 cacgttgagc atctcgggcg tcatcacgac gcggccgctc tcgcggttca gcggcgacag
     4261 gtgctggaac tcgatgtcga gcacggccgc accagccggc acgtcgagcg tgaatgcata
     4321 cgggtcgagc gggtcgcgcc gccatgcgag cgcggcgccg ccggcgctga tctgcagccc
     4381 ggcgaggtcg ccgatgcggc cggtcggccc gtggttgccg ggcagccact gcgggtgata
     4441 cagccgcagc gggccggccg ccaccggcac cgtctcgcgg atgcggaaga tgcgtcggtc
     4501 gaggtcggtc gcgtcgacct gcagggtcaa ggtgccgggg tagagggtgt cggtggggcg
     4561 gggggcttgg gcggtggccg tcgtgtggat cagcaggccc agcacggccg cgctcgtgac
     4621 gcggccgagg gtcgggagca gaaaggtcat cgaggtgcga aggtcgtcga gcgcacgggg
     4681 ggcgcggacc gcacacgata cggcaatcgt cccgcgctgc gactcacgcc gggtcggtga
     4741 tgccggcgcg cggcgcgtcg ggccagtgcg gcgactgcag tgcggggtcc gccgcctcgt
     4801 agccgacctc cgcggcatcg cctcgtgcgg gtgcggtcgg cgcgggctgc tgccggcccg
     4861 agaagtacca ccgccgcacg tctgccaccc agtccacgtc gcttgccact gccgcctcca
     4921 ctgccgtccg tcgaagggaa ggctgaatcg tccgcgagcc gaggctcgtg cgccatcggg
     4981 tggcgggtcg tttcgctgta ggggttcgcc tacgcgacgg agcgggccat gtgtcggcag
     5041 aatcacgcga tgaactccgc cgccctcgat gcgacccgcg tcgcgcccga tgccccgacc
     5101 ccccgccccg agcccgagcg tgcccgggcc ctcgcggtgc gccacttgcg ccaggtgctg
     5161 ctgtggccgc tgcgcctgat gccgatgaag cgcatccccg gccacgcgcg ccgcgcgccg
     5221 tggcaggtgc tgcgcgacct gggcgaggtg tcgccgtggc gcgaggtgat cgacgaatac
     5281 ggcggcgaca gcgggcgctt tcacgagcgc cactacagcg agttcgtgac cttcctgccc
     5341 tatgtgcagc gctttctcta tggcgaaggg cgcaaggcga ggggcgccga gggtcgcgac
     5401 gccgacgcgc cgatgcgcgt gttccgccgc gaggacatcg cccacgtgcg cgtcgttgcg
     5461 cgcccgggcg acgcgcccgt gacgctcgag gtcatccacg tcgatctgta tttcttctac
     5521 gacgtcgaca tcgtgctgct caacctcgag atcggcgcga acgacctgac gctggaccag
     5581 gcgcaggagc agctctaccg cttcggtcgc ggctacccgg cgggctggga cgtgtcgggc
     5641 cacgcgctgc acacgatggc cagcgtcgag tggctcgacg cggccggcgc ggcgctggcg
     5701 cgctccgatg cgcagcagcg tgagcttttc atggcccacg tggccgagca ccgcgcaccg
     5761 cgcaccgcgg cgcactgggc cttcatgctc aagccgctgg tggccgacca ctccgaggag
     5821 cccggcgaac tgcgctaccg gcagatcgag tactaccgga tgccgctgat gggctatctc
     5881 gcgctcgacg acccgcgcgc gctgacccgc gacgacttca ttcgcctcgg cctggtcacc
     5941 ggcgccggcg gcagcgacaa cggcagcctg atcgacgaca ccgacccgcg cgcgaccgat
     6001 gacggcagcg cgctccaagg gcgtcgggac ggcagcgcgc tgccgtatgc cgccgaccac
     6061 ctggccgact tcgagcagcg ctactgctac gaccgtttct gggccaatgc gggcgccgcg
     6121 ccgaacacgc gctacctgtg cagcggccac gcgctgatcg tgatcggcga cgcgcgctcg
     6181 accttctatg gctgtcgcga ccgcggcgtg ctggcgcagt tccgccatca gcacttcctg
     6241 ctgttcctga tcgcgcactt ccagaaggcg gcgctgctga tgctgtccga ccggctggtc
     6301 gtcgcgctgt cgcgcctgga catccaggac gtcgacacca tccgccgctt caagcgcagc
     6361 atccggcaga tgctggagat cttcctgcgc ttcacgcacc gctactggtt ccacgaggtc
     6421 gccgaccagg cgcaggccag ggcgctgttc cggctgtgcg cgcagcacct cgacctcgac
     6481 gggttgtaca gggaggtcaa ggatcggatc tacgacatga acgagtacct ggacagtgac
     6541 agtctgcgcc gacaagccaa taccgtgctc cgcctcacgg tcgtaacgac tttcggcttg
     6601 atcg
//
LOCUS       DASRDG010000041         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1617639, whole genome shotgun sequence.
ACCESSION   DASRDG010000041 DASRDG010000000
VERSION     DASRDG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617639"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFA35_00625"
     CDS             complement(<1..623)
                     /locus_tag="VFA35_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZT54699.1"
                     /translation="MHFNGMDWIAIVSGFGVGAIVGLTGVGGGSLMTPLLLSVFKLNP
                     AVAVGTDLWFAAITKTAGSISHHQAGHVKWRITALLLAGSIPASLATVALMHFTGVTK
                     GWASALTFTLGVALLLTAVTVAYKRVWQGVGLRLERWIPESRKPWLTVGCGLILGVLV
                     SLSSIGAGAIGATLILLLYPRLKSHEIVGTDIAHAVPLTLVAGIGHAT"
     gene            827..1636
                     /locus_tag="VFA35_00630"
     CDS             827..1636
                     /locus_tag="VFA35_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZT54700.1"
                     /translation="MAAPPPPKPAPKPPRIGLALGGGAARGFAHIGVIQVLEENGIKP
                     DLVAGTSAGSLVAALYASGKTGAQLAALADSMDESAFTDWAFPGRGLIRGEGLAKYVR
                     EHTGGLRIEQMHMPLGIVATDLDSGEPILFQRGDPGVAVRASSAVPAVFQPVRIGARE
                     YVDGGLVAPVPVRFARQMGAELVVAVDISAAPDGNPTGDAMRMLLQTFAIMGRSINGF
                     ELRDADVLLRPSLPHVSGADFTARKRAIQAGREAMQAQLGLLRERIAAKMH"
     gene            complement(1729..>2131)
                     /locus_tag="VFA35_00635"
     CDS             complement(1729..>2131)
                     /locus_tag="VFA35_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZT54701.1"
                     /translation="AAENTKAALSAKDAQEFMALQASLLQPAAEKAAAYSRHLYDIAA
                     ATSAEVNKVAEATAAEAQAKFMSAVDTAVKNAPAGSENAVALVKSAVAAANNAFEGVQ
                     KAVKQATETAEANFQAISATATKATKVKRAA"
BASE COUNT          317 a          721 c          766 g          327 t
ORIGIN      
        1 gtcgcgtggc cgatgccggc gaccagcgtg aggggcaccg cgtgggcaat gtcggtgccg
       61 acgatttcgt gcgacttcaa tcgcgggtac agcagcagga tcagcgttgc gccgatcgcg
      121 ccggcaccga tcgagctgag cgagaccagc acgccgagga tcaggccgca gccgaccgtc
      181 agccagggct tgcggctttc gggaatccag cgctccagcc gcagaccgac accttgccag
      241 acgcgcttgt acgcgaccgt gaccgcggtc agcagcagcg cgacaccgag cgtgaaggtc
      301 agcgccgaag cccagccctt ggtcacgccg gtgaagtgca tcagcgcgac cgtcgcgagc
      361 gaggccggga tgctgcccgc cagcagcagg gcggtgatgc gccatttgac gtggccggct
      421 tggtggtgcg agatcgagcc ggcggtcttc gtgatcgcgg cgaaccacag gtcggtgccg
      481 accgcgacgg ccgggttcag cttgaacacg gacagcagca gcggcgtcat cagcgagccg
      541 ccgccgacgc cggtcaagcc gacgatggcg ccgacgccga agccgctgac aatggcgatc
      601 cagtccatac cgttgaagtg cacgaagccg cgccccagcg gcgccgaagc acgcgactgt
      661 acggagtact catatattct ggaagaacat tatcaaattt tgcttattcg atgacggcat
      721 aagaaccgcg gcgctgaccg agcgccgccg ctggctgctg gcgggcggcg ccgcagcggc
      781 gctggtgctg gccggctgcc agaccgctcc cccgcccgcg cctgtggtgg ctgcgccacc
      841 gccgccgaaa ccggccccga agccgccacg catcggcctc gcgctcggcg gcggcgcggc
      901 gcgtggcttc gcacacatcg gcgtgatcca ggtgttggag gaaaacggca tcaagcccga
      961 cctggtcgcg ggcacctcgg ccggcagcct ggtcgcggcg ctctacgcct cgggcaagac
     1021 cggcgcccaa ctcgccgcgt tggccgactc gatggacgaa tcggccttca ccgactgggc
     1081 ctttcccggc cgcggcctga tccgcggcga gggcctcgcg aagtacgtgc gcgaacacac
     1141 cggcggcctg cggatcgagc agatgcacat gccgctgggc atcgtcgcga ccgatctgga
     1201 cagtggcgag ccgatcctgt tccagcgcgg cgacccgggc gtcgcggtgc gcgcgtcgag
     1261 cgcggtgccg gcggtgttcc agccggtgcg catcggcgcg cgcgagtacg tcgacggcgg
     1321 cctggtcgcg ccggtgccgg tgcgcttcgc gcggcagatg ggcgccgagc tcgtggttgc
     1381 ggtcgacatc tcggccgcgc ccgacggcaa cccgaccggc gacgcgatgc gcatgttgct
     1441 gcagaccttc gcgatcatgg gccgcagcat caacggcttc gagttgcgcg acgccgacgt
     1501 gctgctgcgc cccagcctgc cgcacgtgtc gggcgccgac ttcacggccc gcaagcgcgc
     1561 gatccaggcc gggcgcgagg cgatgcaggc gcagctcggg ctgctgcgcg agcgcatcgc
     1621 cgcgaagatg cactgagcga atcgaggccc gacaaagcaa acggccccga agcatcgctg
     1681 cttcggggcc gacaggtggc tcggcgaggg ccgagcgacc gagcgagatc aggcggcgcg
     1741 cttgaccttg gtggccttgg tcgcggtggc gctgatcgcc tggaagttgg cttccgcggt
     1801 ttcggtcgct tgcttgacgg ccttttgcac gccttcgaac gcgttgttcg cagcggcgac
     1861 agccgacttg accagcgcca cggcgttctc gctgccggcc ggcgcgttct tcacggcagt
     1921 gtcgacggcc gacatgaact tggcttgcgc ttcggcggcg gtcgcttcgg cgaccttgtt
     1981 gacttcggcg ctggtcgcgg cggcgatgtc atacaggtgg cggctgtagg cggcggcctt
     2041 ctcggcagcc ggttgcagca ggctggcttg cagggccatg aattcttgtg cgtccttggc
     2101 cgacagcgcg gccttggtgt tctcggcggc t
//
LOCUS       DASRDG010000042         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_582088, whole genome shotgun sequence.
ACCESSION   DASRDG010000042 DASRDG010000000
VERSION     DASRDG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582088"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VFA35_00640"
     CDS             complement(<1..1007)
                     /locus_tag="VFA35_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HZT54702.1"
                     /translation="MPATCQVVVGCIAACVCACVGAQPVLPAQAASAAAREEAPASLP
                     DLLLPAVEIVGFELLVNRANRAFGTGRDDYRVTAGSIRRNLRSGWGTDHDPFDINQLA
                     HPVQGAMYHGFARSAGLGYWPSLGMTFAGSALWEVAGERTLPSRNDQVASGIGGSFLG
                     EALFRMSNLVLERGGGLPRFWREAAAAAIAPSAGFNRLVFGERAGGVFSSRDAAYFSR
                     LEFGYGGSASHRPGTSNAAFKRNEAQLGFAIDYGMPGSPGYTYTRPFDYFAFQATATS
                     ASGIENVLTRGLLVGRGYELGATGRGVWGLYGSYDYIAPQTFRVSTTALSLGSTAQWW
                     FG"
     gene            complement(1107..2018)
                     /locus_tag="VFA35_00645"
     CDS             complement(1107..2018)
                     /locus_tag="VFA35_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZT54703.1"
                     /translation="MKTFKGRTAVITGAASGFGLEASRIAAGLGMNIVMADVQQDALD
                     RAAAEIEALGAPVLAFRLDVSRADEVEALGAATFKRFGAPHFVFNNAGVGAGGLIWET
                     TLKDWEWLVGVNLMGVAHGIRVFTPMMLAAAAKDPGYEGHITNTASMAGLLNPPNMGV
                     YNATKSAVVAMSETLYQDLKLVTDQISASVLCPFFVPTGITQSERNRPAAMTDAGSPP
                     TRSQLIGQAMIDKAVSSGKVSAAEVAQFVFDAIAQQRFYVYSHPKSLASVQVRLEDIL
                     TPRNPTDPFAHKPELGEQLRAQLRAVG"
BASE COUNT          321 a          756 c          761 g          344 t
ORIGIN      
        1 ccgaaccacc attgcgcggt cgagcccagc gacagcgcgg tggtcgagac gcgaaaggtc
       61 tgcggtgcga tgtagtcgta gctgccgtaa aggccccaca cgccgcgccc ggtcgcaccg
      121 agctcgtagc cgcggccgac cagcagcccg cgcgtcagca cgttctcgat gccgctggcg
      181 ctcgtcgccg tggcctggaa cgcgaagtag tcgaacggcc gcgtgtaggt gtagccgggg
      241 ctgccgggca tgccatagtc gatcgcgaag ccgagctgcg cctcgttgcg cttgaacgcc
      301 gcgttcgacg tgcccggcct gtgcgaagcg ctgccgccgt agccgaactc gagccggctg
      361 aagtacgccg cgtcgcggct cgagaagacg ccgccggcgc gctcgccgaa gaccagccgg
      421 ttgaatcccg ccgacggcgc gatcgccgcg gcggccgcct cgcgccagaa gcgcggcagg
      481 ccgccgccac gctcgagtac gaggttcgac atgcggaaca gcgcctcgcc gagaaagcta
      541 ccgccgatgc cgctggcgac ctggtcgttg cgcgacggca gggtgcgttc gcccgcaacc
      601 tcccacaacg cgctgcccgc gaaggtcatg ccgagcgagg gccagtagcc cagccccgcc
      661 gagcgcgcga agccgtggta catcgcaccc tgcaccgggt gggcgagctg gttgatgtcg
      721 aacggatcgt ggtcggtgcc ccaaccgctg cgcaggttgc gccggatgct gcccgccgtg
      781 acccggtagt cgtcgcgccc ggtaccgaac gcgcgattcg cgcgattcac cagcagctcg
      841 aagccgacga tttcgaccgc gggcagaagc agatccggca gcgatgctgg cgcttcttca
      901 cgcgccgcgg cgcttgccgc ttgcgccggc agcaccggct gggccccgac gcaggcacac
      961 acgcatgctg caatgcagcc gacgacaacc tgacacgttg ccggcatggc gctctccgga
     1021 ggctggacgt ggcccgagtc tgcggttgtc gctccggccg cagcgtcggc agacgacgct
     1081 gcgcgttgta ggacggcgct tggctttcag ccgaccgcgc gcagctgcgc ccgcaactgc
     1141 tcgcccagct cgggcttgtg cgcgaacggg tcggtcgggt tgcgcggcgt caagatgtct
     1201 tcgagccgca cctgcacgct ggcgagcgat ttcgggtggc tgtagacgta gaagcgctgc
     1261 tgtgcgatcg cgtcgaacac gaactgcgcg acctcggcgg cgctgacctt gccgctgctc
     1321 acggccttgt cgatcatcgc ctggccgatc agctggctgc gcgtgggcgg gctgcccgcg
     1381 tcggtcatcg cggccgggcg gttgcgctcg ctttgcgtga tgccggtcgg cacgaagaac
     1441 gggcacagca ccgaggcgct gatctggtcg gtgacaagct tcaggtcctg gtacagcgtc
     1501 tcgctcatcg cgacgaccgc gctcttggtc gcgttgtaga cgcccatgtt gggtgggttc
     1561 agcaagcccg ccatcgaggc ggtgttggtg atgtggccct cgtagcctgg gtctttcgcg
     1621 gcggcggcca gcatcatcgg cgtgaacacg cggatgccgt gcgccacgcc catcaggttg
     1681 acgccgacga gccactccca gtccttcagc gtcgtctccc agatcaggcc gccggcgccg
     1741 acgccggcgt tgttgaagac aaaatggggc gcgccgaagc gcttgaaggt ggcggcgccg
     1801 agcgcttcta cttcatcggc cctggacacg tcgagccgga acgcgaggac cggcgcgccg
     1861 agcgcctcga tctcggccgc ggcccggtcg agcgcgtcct gctgcacgtc ggccatcacg
     1921 atgttcatgc ccaggcccgc ggcgatgcgc gacgcttcga ggccgaagcc ggatgccgcg
     1981 ccggtgatca cggccgtgcg gcccttgaag gttttcatcg acagtctctc tcggttcaaa
     2041 cagtgggtgg cttcttgatc tggaagccga tgcgcgggaa gtgcacctgc accgcgccgg
     2101 cgcgttcgtc ggtgcgcgcg atcacgacct cctgcgtgct caggccgaca agccggcccg
     2161 cgaccgcgtc gtacgcgtag tc
//
LOCUS       DASRDG010000043         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_702762, whole genome shotgun sequence.
ACCESSION   DASRDG010000043 DASRDG010000000
VERSION     DASRDG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702762"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VFA35_00650"
     CDS             complement(<1..1578)
                     /locus_tag="VFA35_00650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZT54704.1"
                     /translation="MRITWLLEQADQIWGGIKVALEDANWLSARGHRVTVLSRSGPPA
                     WMALRCQFRTVPDFRTERLPEADVLIGTWWSTMPAVAAAGAGRGAPVHFCQGYEGDFP
                     EQAPQRESIEAAYRLPGVHHIAISPHVAALLQRRFRLTARAIPNAIDHSVHYPGAARP
                     PGGMLRIGLVGPWQVPWKDLATGVEACRRAAAAGQPLQLVRVTNTQPDAAELDLPFPV
                     EWHQRVAPRQMGDIYRSLDVFLGTSSGPEEGFHLPAVEAMACGVPCVLTDVPCFRAHG
                     DGQYAMFVPPRDPAAMAEALVVAGRVDDVRSALRTAGIAAAARYTQQAHGQALEQALL
                     AIAVQGRAQTRSALRAPPMPADADAGTAARTDAPAGAQEPGPAGDLDGLEAELARSLQ
                     RAGERAARAGEPARADRLRAAARCLSPVAAPAGDAPWRRWLVGDKAAALQKLDELAAS
                     GVDDPALHEQRGLLLHDCGRDLEAAQSFRAAIAAGGASADAFNNLGVALWETGDVAGA
                     RASFERALHLQPTHRDAT"
     gene            1678..2595
                     /locus_tag="VFA35_00655"
     CDS             1678..2595
                     /locus_tag="VFA35_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZT54705.1"
                     /translation="MKPLIRLIPPFLVRFFARPYVAGDSLEQAIATVDRLWNGRSLLA
                     TLDLLAEDIRLQEQADQNLATYRRMVAAASALPAPWRPTLSLKPSSYTTRPLQAGGDA
                     AGSLAAVTAIAEDGARTGVGITIDMESRHWTDFTLAALQKLHADGHRHVGAVLQSRLH
                     RTEHDLDRLPAGMRVRLVIGIYREPAEHALTDKPAMKLRLLTYAERLLRAGHYVEFAT
                     HDEACVRRFVDDVVPRCGVGADRYEVQMLYGVPRERLLGELRQRGIRARLYVPFALSW
                     AMAIQYLRRRLDEYPAMMWLVSKNLIRRR"
BASE COUNT          366 a         1073 c         1091 g          393 t
ORIGIN      
        1 ggtcgcgtcg cggtgggtcg gctgcaggtg cagcgcgcgc tcgaagctcg cgcgcgcgcc
       61 ggcgacgtcg cccgtctccc atagcgcgac gccgaggttg ttgaacgcgt cggcgctcgc
      121 gccgccggcg gcaatcgcgg cacggaacga ctgcgccgcc tcgaggtcgc ggccgcagtc
      181 gtgcagcaac agcccgcgct gttcgtgcag cgcggggtcg tcgacacccg acgcggccag
      241 ctcgtcgagc ttctgcagcg cggccgcctt gtcgccgacc agccagcggc gccacggcgc
      301 gtcgccggcc ggcgccgcga cgggcgagag gcagcgcgcg gcggcgcgca accgatcggc
      361 ccgcgccggt tcgccggcgc gcgcggcgcg ttcgcccgcc cgctgcagcg aacgcgcgag
      421 ctccgcctcg aggccgtcga ggtcgcccgc gggccccggc tcctgcgcgc cggccggcgc
      481 gtcggtgcgg gcggccgttc ccgcgtccgc gtccgcgggc atgggcggcg cgcgcagcgc
      541 gctgcgggtc tgcgcgcggc cctgcaccgc gatcgcgagc agcgcctgtt cgagcgcctg
      601 gccgtgcgcc tgctgcgtgt agcgcgcggc agcggcgatg cccgcggtgc gcagcgcact
      661 gcgcacgtcg tcgacgcggc cggcgaccac cagcgcctcc gccatcgccg ccggatcgcg
      721 cggcggcacg aacatcgcgt actggccgtc gccgtgggcg cggaagcacg gcacgtcggt
      781 gagcacgcac ggcacgccgc acgccatcgc ctcgaccgcc ggcaggtgga agccctcctc
      841 ggggcccgac gaagtgccga ggaagacgtc gagcgaacgg tagatgtcgc ccatctgccg
      901 cggcgccacg cgctggtgcc attcgacggg gaacgggagg tcgagctcgg cggcgtcggg
      961 ctgcgtgttg gtgacgcgca cgagctgcag cggctggccg gcggcggccg cgcgccggca
     1021 cgcctcgacg ccggtggcga ggtccttcca cggcacctgc cacggaccga cgagaccgat
     1081 gcgcagcatg ccgcccggcg gccgcgcggc gcccgggtag tgcacggaat ggtcgatcgc
     1141 gttcgggatc gcgcgcgcag tcaggcggaa gcggcgctgc agcagcgcgg ccacgtgcgg
     1201 cgagatcgcg atgtggtgca cgcccggcag ccggtaggcc gcctcgatgc tctcgcgctg
     1261 cggcgcctgc tcggggaagt cgccttcgta gccctggcag aagtgcaccg gggcgccgcg
     1321 gccggcgccg gccgcggcga cggcgggcat cgtcgaccac caggtgccga tcagcacgtc
     1381 ggcctccggc aggcgctcgg tgcggaagtc gggcacggtg cggaactggc agcgcagcgc
     1441 catccacgcc ggcgggccgc tgcggctcag caccgtcacg cggtggccgc gcgccgacag
     1501 ccagttcgcg tcctcgagcg cgaccttgat gccgccccag atctggtcgg cctgctccag
     1561 cagccacgtg attcgcacgc cttcccatcg gcgtttgcgc ggtcgccgtt tgagccgcgg
     1621 gcgccgccgc tgctcggttg gattccggcg ccgcggtcgc catgatccgc ggcgctcgtg
     1681 aaaccgctga tccgcctgat tccgccgttc ctcgtgcgct tcttcgcgcg cccgtacgtc
     1741 gccggcgatt cgctggaaca ggcgatcgcg acggtggatc ggctgtggaa cgggcgctcg
     1801 ttgctggcca cgctcgacct gctcgccgag gacatccgcc tgcaggaaca ggcggaccag
     1861 aacctcgcga cctaccggcg catggtcgcg gcggcgtcgg cgctgccggc gccgtggcgg
     1921 ccgacgctgt cgctgaagcc gtcgtcgtac accacgcggc cgctgcaggc gggcggcgat
     1981 gcggctggtt cgctcgcggc ggtcacggcg atcgccgagg acggcgcgcg aaccggggtc
     2041 ggcatcacga tcgacatgga gagccggcac tggaccgact tcacgctcgc ggcgctgcag
     2101 aagctgcacg cagacggtca ccggcacgtc ggcgccgtgc tgcagtcgcg cctgcaccgc
     2161 accgagcacg acctcgacag gctgcccgcg ggcatgcgcg tgcggctcgt gatcggcatc
     2221 taccgcgagc ccgccgagca cgcgctcacc gacaagccgg cgatgaagct gcggctgctc
     2281 acgtatgcgg agcggctgct gcgcgcaggc cactacgtcg agttcgcgac gcacgacgag
     2341 gcgtgcgtgc gcaggttcgt ggacgacgtc gtgccgcgct gcggggtcgg cgccgaccgc
     2401 tacgaggtgc agatgctgta cggagtgccg cgcgagcggt tgctcggcga actgcgccag
     2461 cgcggcatcc gcgcgcggct gtacgtgccg ttcgcgctca gctgggcgat ggcgatccag
     2521 tacctgcgcc ggcggctcga cgagtacccg gcgatgatgt ggctcgtcag caagaacctg
     2581 atccggcgcc gctgatctcc tggctgcgcg agtcatcgcc gcgcgcgcga cgattccgcg
     2641 gcccgcgtgc cgctccggtt gctgcgcagg cagaagtcag ccgcaggcag cgctgaaccc
     2701 ccgttgccgc ggtttttccc ggctgcgcta ggtcctctgg tcgtgccttg ctacaatcct
     2761 cggcccccta tggatccact cggcaccatt gtcgaacgac tctgcgcctg gaaggacgtg
     2821 gcccgcgaga agcgggacaa caaagactgc ttcctcgtgc gcggccaggt gttcgcctac
     2881 ctcggcaaga agggcgtggt cgtcaagctg gcgccgccac agg
//
LOCUS       DASRDG010000044         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_812349, whole genome shotgun sequence.
ACCESSION   DASRDG010000044 DASRDG010000000
VERSION     DASRDG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812349"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFA35_00660"
     CDS             complement(<1..193)
                     /locus_tag="VFA35_00660"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZT54706.1"
                     /translation="MLAQRTLKAMTRAVGVGLHGGQRVELTLRPAAPDTGIVFRRVDL
                     AQPVDIRVNALWITDTRMAT"
     gene            complement(246..1454)
                     /gene="ftsZ"
                     /locus_tag="VFA35_00665"
     CDS             complement(246..1454)
                     /gene="ftsZ"
                     /locus_tag="VFA35_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZT54707.1"
                     /translation="MAIEMIEEFSMGTQIKVIGVGGGGGNAVEHMITQGVQGVQFVCA
                     NTDAQALNRSAAHDLIQLGTTGLGAGAKPECGKAAAEEAVDRIRQAIDGSHMLFITAG
                     MGGGTGTGAAPVIARVAKEMGILTVGVVTKPFEFEGNRRMKAADAGLAELEANVDSLI
                     VILNDKLLDTLGDDITQDQAFAHANDVLRNAVGGISDIIHMPGLVNVDFEDVKTVMSE
                     PGKAMMGTAQASGPDRATKAADAAVACPLLEGIDLSGARGVLVLIAANRNTFKLAESR
                     NAMNTIRRYAADDAHVIYGTAYDESLGDQLRVTVIATGLSPARRQQSPISVVHSMPAQ
                     RTGTHDLPILQNALNTAPQGIAPAHDYSALTTPSVWRNGRTAAAKVDALASNGMDEIE
                     IPAFLRKQAD"
     gene            complement(1504..1626)
                     /locus_tag="VFA35_00670"
     CDS             complement(1504..1626)
                     /locus_tag="VFA35_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54708.1"
                     /translation="MKHTHSLLHVQSAHGLAPPRAGPARRRKRRTTLGDNKIDL"
     gene            complement(1644..2873)
                     /gene="ftsA"
                     /locus_tag="VFA35_00675"
     CDS             complement(1644..2873)
                     /gene="ftsA"
                     /locus_tag="VFA35_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZT54709.1"
                     /translation="MAKEYKDLVVGLDIGTAKIMVVVAEVMPDGELRIAGLGSAPTHG
                     LKRGVVVNIDATVQSIQQALKEAEMMADCKITRVYTGITGSHIRGQNSTGMVIVRDKE
                     VTPVDVARVVETAKAINIPNDQRLLLVEPQEFVIDGHEVKEPIGMSGGRLEVKVHIVT
                     GAQSAAENIVKCVRRCGLEVDQLVLNPSASSHAVLTDDEKDLGVALVDIGAGTTDVAI
                     FTDGAIRHTAVIPIAGDLITSDIAMALRTPTKDAEEIKVEYGVAKQLLADPGDQLEVP
                     GLGDRAPRMLSRQALAGVIEPRIEEIYSLVHQVIRESGYEELLSSGIVITGGAAVMPG
                     MVELGEDIFLKPVRKGVPTYHGALYDMVNNPRSATVMGLLEEARIGRSRGMRAAQQAG
                     SVKTLFGRAKDWFLGNF"
     gene            complement(2892..>3210)
                     /locus_tag="VFA35_00680"
     CDS             complement(2892..>3210)
                     /locus_tag="VFA35_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HZT54710.1"
                     /translation="SSAQMLKMVGRLQPEFAAMDARIDTLELSGRGSWRVELDSGAEV
                     ELGRGSDDEVIARTKSFIATLPELRNRYAQHPLLYADLRHNDGYAVRLKGISTSADAA
                     KKN"
BASE COUNT          411 a         1105 c         1079 g          615 t
ORIGIN      
        1 tggtcgccat gcgcgtgtcg gtgatccaca gcgcgttgac gcggatgtcg accggctgcg
       61 ccagatcgac gcggcgaaac acgatgccag tatcgggcgc ggccgggcgc agcgtcagtt
      121 cgacgcgctg gccgccatgc aggccgacgc cgaccgcgcg ggtcatggct ttgagagtgc
      181 gttgcgcgag catggtccga attgtcctgg aaaactcgaa ccgcgcccgc gccccgaata
      241 tcacctcagt cggcctgctt gcggaggaac gccgggatct cgatctcgtc catcccgttg
      301 ctcgccagcg cgtcgacctt cgcggccgcg gtgcggccgt tgcgccagac gctcggcgtc
      361 gtcagcgcgc tgtagtcgtg ggccggcgcg atgccttggg gtgcggtgtt cagcgcgttc
      421 tgcaggatcg gcaggtcgtg cgtgccggtg cgctgcgccg gcatgctgtg gaccacgctg
      481 atcggcgact gctggcggcg cgccggcgac aggccggtgg cgatcacggt cacgcgcagc
      541 tggtcgccca ggctctcgtc gtacgcggtg ccgtagatca cgtgcgcgtc gtcggcggcg
      601 tagcgacgga tcgtgttcat cgcgttgcgg ctctcggcca gcttgaaggt gttgcggttc
      661 gccgcgatca gcaccagcac gccgcgcgca cccgagaggt cgatgccttc gagcagcggg
      721 caggccacgg ccgcatcggc cgccttggtc gcgcggtccg ggccgctggc ctgcgccgtg
      781 cccatcatcg ccttgcccgg ctcgctcatc acggtcttca cgtcctcgaa gtcgacgttg
      841 accaggcccg gcatgtggat gatgtcgctg atgccgccga cggcgttgcg cagcacgtcg
      901 ttggcgtgcg cgaaggcctg gtcctgcgtg atgtcgtcgc cgagcgtgtc gagcagcttg
      961 tcgttcagga tcacgatcag cgagtcgaca ttcgcttcga gctcggcgag cccggcgtcg
     1021 gccgccttca tgcggcggtt gccctcgaac tcgaacggct tggtgacgac gccgacggtc
     1081 aggatgccca tttccttcgc gacgcgcgcg atcaccggcg cggcgccggt gccggtgccg
     1141 ccacccatgc cggcggtgat gaacagcatg tgcgagccgt cgatggcctg acggatgcgg
     1201 tccaccgcct cttcggccgc ggccttgccg cattcgggct ttgcgcccgc gcccaggccc
     1261 gtggtgccga gctgaatcag gtcgtgcgcg gccgagcggt tcaaggcctg cgcgtcggtg
     1321 ttggcgcaga cgaactgcac gccttgcacg ccttgcgtga tcatgtgctc gaccgcgttg
     1381 ccgccgccgc cgccgacgcc gatcaccttg atctgcgtgc ccatgctgaa ctcttcaatc
     1441 atttcgatgg ccatttgttt ctccagtctt tcggttgcag ttgccgtgtg tgtgtgtgtg
     1501 tgttcagaga tcgatcttgt tgtcgcccaa cgtcgtcctt cgttttcgtc gtcgcgccgg
     1561 tccggcgcgc ggcggcgcca gcccgtgggc ggattgaacg tgcagcaatg aatgggtgtg
     1621 cttcatcgtg tttccgcctc gctttagaaa ttgcccagga accagtcttt cgcgcggccg
     1681 aacaaggtct tgaccgaccc cgcctgctgc gccgcccgca tgccgcgcga gcgcccgatg
     1741 cgcgcctctt cgagcaaccc catcacggtc gccgagcgcg ggttgttgac catgtcgtag
     1801 agcgcgccgt ggtaggtcgg cacgcccttg cgcaccggct tgaggaagat gtcttcgccg
     1861 agctcgacca tgcccggcat caccgcggcg ccgccggtga tgacgatgcc gctggacagc
     1921 agctcctcat agccgctctc gcggatcacc tggtgcacga gcgaatagat ctcctcgatg
     1981 cgcggctcga tcacaccggc cagcgcctgg cggctgagca tgcgcggcgc gcggtcgccc
     2041 agccccggca cctcgagctg gtcgcccggg tcggccagca gctgcttggc gacgccgtac
     2101 tcgaccttga tctcctcggc gtccttggtc ggcgtgcgca gcgccatcgc gatgtcgctg
     2161 gtgatcaggt cgccggcgat cgggatcacc gcggtgtggc ggatcgcgcc gtcggtgaag
     2221 atcgccacat cggtggtgcc agcgccgatg tcgaccagcg cgacgcccag gtccttctcg
     2281 tcgtcggtca gcaccgcgtg gctcgacgcg ctcgggttca gcacgagttg atccacttcg
     2341 agcccgcagc gccgcacgca cttgacgatg ttctcggccg cgctctgcgc gccggtgacg
     2401 atgtgcacct tgacctcgag ccggccgccg ctcatgccga tcggctcctt gacctcgtgg
     2461 ccgtcgatca cgaactcctg cggctcgacc agcagcaggc gctggtcgtt cgggatgttg
     2521 atcgccttcg cggtctcgac gacgcgcgcc acgtcgaccg gcgtgacctc cttgtcgcgc
     2581 acgatcacca tgccggtcga gttctggccg cggatgtggc tgccggtgat accggtgtag
     2641 acgcgcgtga tcttgcagtc ggccatcatc tcggcctctt tcagggcctg ctggatgctc
     2701 tgcaccgtcg cgtcgatgtt cacgaccacg ccgcgcttca ggccgtgcgt gggcgcgctg
     2761 ccgaggccgg cgatgcgcaa ctcgccgtcc ggcatcacct cggccaccac caccatgatc
     2821 ttggcggtgc cgatgtcgag gccgaccacc agatccttgt attccttggc catgtgcgtg
     2881 ttcccttccc tctagttctt cttggcggcg tcggccgacg tggagatgcc cttcagccgt
     2941 accgcgtagc cgtcgttgtg gcgcaggtcg gcgtacagca gcgggtgctg cgcgtaacgg
     3001 ttgcgcagct cgggcagcgt cgcgatgaag ctcttggtgc gcgcgatcac ctcgtcgtcg
     3061 ctgccgcgcc cgagctcgac ctcggcgccg ctgtcgagct cgacgcgcca cgagccgcgg
     3121 cccgagagtt cgagcgtgtc gatgcgcgcg tccatcgcgg cgaactcggg ctgcagccgg
     3181 cccaccatct tcagcatctg cgccgagctg
//
LOCUS       DASRDG010000045         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_467703, whole genome shotgun sequence.
ACCESSION   DASRDG010000045 DASRDG010000000
VERSION     DASRDG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467703"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFA35_00685"
     CDS             complement(<1..191)
                     /locus_tag="VFA35_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZT54711.1"
                     /translation="MEKALPSIPAKRYFTIGEVSDLCGVKPYVLRYWEQEFTQLKPMK
                     RRGNRRYYQHHEVLLIRRIR"
     gene            complement(227..625)
                     /locus_tag="VFA35_00690"
     CDS             complement(227..625)
                     /locus_tag="VFA35_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HZT54712.1"
                     /translation="MNSTDPRDAGDGDEAEAPDTNRVALPTLETPTLTKAELAELLFE
                     RLGLNKRESKDMVEAFFDLIHATLVSGADVKMSGFGNFNIRRKAPRPGRNPRTGEAIP
                     IKARNVVTFHASHKLKAVVQGDIPPEEEFE"
     gene            complement(639..>2680)
                     /gene="pheT"
                     /locus_tag="VFA35_00695"
     CDS             complement(639..>2680)
                     /gene="pheT"
                     /locus_tag="VFA35_00695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZT54713.1"
                     /translation="ARELKLSEEHGGLLILDKDAIVGQNIREHLSLDDTLLTLKLTPN
                     LAHCLSVYGVARELSALTGAPLRQPSFPPVAPRFDDKLPVTIAAPDLCGRFSGRIIRN
                     VNTKAPTPAWMVDRLARCGQRSVTALVDISNYVMFEYGRPTHIFDLDKIHDGLIVRWG
                     RPGETLKLLNGATITVDEQVGVIADSQAVESLAGIMGGDATAVSDATKNVYVEAAFWW
                     PEAVMGRSRRFNFTTDAGHRFERGVDPALTVEHIERITQLIIEICGGEAGAMDDQVLR
                     LPEATPVTLRVARAAQVIGMPITQAQCESVMRRLGFAFTSAPGTITVTPPSWRFDLKL
                     EEDLIEEVIRIVGYDSLPTAPPLAALVPRVPSESQRSLDALRHQMAALDYQETVNFSF
                     VDARWEHELAGNADPIKLVNPMASHLAVMRSSLLGSLIGVLRLNVSRKATRVRVYELG
                     RAFTRDASAVDGPASVAGVGQAMRLAGLAYGGADALQWGTKERNVDFFDLKGDIEALL
                     APRRARFVAAEHPAMHPGRCARIELDGAAIGFAGELHPKWRQAYELPHAPLLFELDAA
                     ALTARDLPSPVPVQRQQSVWRDLSVIAGEAVTHDALMQAIAGAKQAGLVKSTRLFDIY
                     KPGAASGDIKAGERSLSIRLELLDDEFTLTDERIDAAVADVLAALGERLGVRLRA"
BASE COUNT          386 a          916 c          928 g          450 t
ORIGIN      
        1 cgaatccgcc ggatcagcag cacctcgtgg tgctggtagt agcggcggtt gccgcgccgc
       61 ttcatcggct tgagctgggt gaattcctgc tcccaatagc gcagcacgta cggcttcacg
      121 ccgcacaagt cgctgacctc accgatcgtg aagtagcgtt tggcgggtat cgaagggagc
      181 gctttctcca tgaaaatcaa tgagttcagg gcagaaagct aagactttac tcgaactcct
      241 cctcgggcgg tatgtcacct tgcacgaccg ccttcaactt gtggctggcg tgaaatgtga
      301 ccacgttgcg tgccttgatc gggatcgcct cgccggtgcg cgggttgcgc cccgggcgcg
      361 gtgccttgcg ccggatgttg aagttgccga agccggacat cttcacgtcg gcgcccgaca
      421 cgagggtggc gtggatcagg tcgaagaagg cttcgaccat gtccttggac tcgcgcttgt
      481 tcaggccgag gcgctcgaac agcagttcgg cgagctcggc cttggtcagc gtcggcgtct
      541 cgagcgtcgg cagcgcgacg cggttggtgt cgggtgcctc ggcttcgtcg ccgtcgccgg
      601 cgtcgcgcgg atcggtgctg ttcatcggcg cgcgctcctc aggcccgcag ccgcacgccg
      661 aggcgctcgc ccagcgcggc gagcacgtcg gcgaccgccg cgtcgatgcg ctcgtcggtc
      721 agcgtgaact cgtcgtccag cagttcgagc cggatgctca ggctgcgctc gccggccttg
      781 atgtcgccgc tggccgcgcc cggcttgtag atgtcgaaca ggcgcgtcga cttgacgagg
      841 ccggcctgct tcgcacccgc aatcgcctgc atcagcgcgt cgtgcgtgac ggcctcgccc
      901 gcgatcaccg acagatcgcg ccagaccgac tgctggcgct gcaccggcac cgggctcggc
      961 agatcgcgcg cggtgagcgc agccgcgtcg agttcgaaca gcagcggcgc atgcggcagc
     1021 tcgtaggcct ggcgccactt cgggtgcagc tcgccggcga agccgatcgc cgcgccgtcg
     1081 agttcgatgc gggcgcagcg gcccgggtgc atcgccgggt gttcggccgc gacgaagcgc
     1141 gctcggcgcg gcgcgagcag cgcctcgatg tcgcccttca ggtcgaagaa gtcgacgttg
     1201 cgctccttcg tgccccactg cagcgcgtcg gcaccgccgt aggccaggcc ggccagccgc
     1261 atcgcctggc cgacgccggc cacgctggcc gggccatcga ccgcgctggc atcgcgcgtg
     1321 aacgcgcgcc cgagctcgta cacgcgcacg cgcgtcgcct tgcgcgagac gttcaggcgc
     1381 agcacgccga tcaggctgcc gagcaggctc gagcgcatca cggccaggtg gctggccatc
     1441 gggttgacca gcttgatcgg gtcggcgttg ccggcgagct cgtgctccca gcgtgcgtcg
     1501 acgaagctga agttgaccgt ctcctgataa tcgagcgcgg ccatctggtg gcgcagcgcg
     1561 tcgaggctgc gctgcgattc gctgggcacg cgcggcacca gcgcggccag cggcggcgcg
     1621 gtcggcaggc tgtcgtagcc gacgatgcgg atcacttcct cgatcaggtc ttcctcgagc
     1681 ttgaggtcga agcgccagct cggcggcgtc accgtgatcg tgcccggcgc gctcgtgaac
     1741 gcgaagccga gccggcgcat cacgctttcg cactgcgctt gcgtgatcgg catgccgatg
     1801 acctgggcgg cgcgcgcgac gcgcagtgtg acgggcgtcg cctcgggcag gcgcagcacc
     1861 tggtcgtcca tcgcgccggc ctcgccgccg cagatctcga tgatcaattg cgtgatgcgc
     1921 tcgatgtgtt cgaccgtcaa cgccggatcg acgccgcgct cgaaacggtg gccggcatcg
     1981 gtcgtgaagt tgaagcggcg cgaccgcccc atcaccgcct ccggccacca gaacgcggcc
     2041 tcgacgtaga cgttcttcgt ggcgtctgag accgccgtcg cgtcgccacc catgatgccg
     2101 gcgagcgatt cgacggcttg cgaatcagcg atcacgccga cttgctcgtc gaccgtgatc
     2161 gtcgcgccgt tcagcagctt cagcgtctcg cccggccggc cccagcgcac gatcaaaccg
     2221 tcgtggatct tgtcgagatc gaagatgtgc gtcggccggc cgtactcgaa catcacgtag
     2281 ttcgagatgt cgaccagcgc ggtcaccgaa cgctggccgc agcgcgcgag gcggtcgacc
     2341 atccacgccg gcgtgggcgc cttcgtgttc acgttgcgga tgatgcggcc cgagaagcgg
     2401 ccgcacaggt caggcgctgc tatcgtcacc ggcagcttgt cgtcgaatcg cggcgccacc
     2461 ggcgggaagc tcggctgccg cagcggcgca ccggtcagcg ccgacagctc gcgcgcgacg
     2521 ccgtagacgc tcaggcagtg cgcgaggttc ggcgtcagct tcagcgtcag cagcgtgtcg
     2581 tcgagcgaca ggtgctcgcg gatgttctgg ccgacgatgg cgtccttgtc caggatcagc
     2641 aggccgccgt gctcctccga gagcttgagc tcgcgcgccg
//
LOCUS       DASRDG010000046         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_243522, whole genome shotgun sequence.
ACCESSION   DASRDG010000046 DASRDG010000000
VERSION     DASRDG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243522"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VFA35_00700"
     CDS             <1..123
                     /locus_tag="VFA35_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54714.1"
                     /translation="KTGAPSRSDRVAKYNQLLRIEEALGAQAKYAGRDAFPIRL"
     gene            159..575
                     /locus_tag="VFA35_00705"
     CDS             159..575
                     /locus_tag="VFA35_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZT54715.1"
                     /translation="MKWFAAALLAALLMLQYRIWFSGDGVREVLRLRTAVAGQQGENE
                     RLSARNRQLAAEVRDLKQGYSALEERARSDLGMIGADETFFQVGPGDAPTAAPGNSPA
                     PGNPAPSAVPTAPRTPGGGPATAPVLAPPVRTAAAR"
     gene            598..1338
                     /gene="ispD"
                     /locus_tag="VFA35_00710"
     CDS             598..1338
                     /gene="ispD"
                     /locus_tag="VFA35_00710"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZT54716.1"
                     /translation="MVAVRYWLVMPAAGASRRFGTAQSKLERSLAGRTVLDLALEPFM
                     DDERCRGIALALNPDGLANAEVRARLGRKVLPIAGGERRCDSVLAGLAALSMAEADDW
                     VLVHDAARPCLSAADLAALLAAGAQHAVGALLAEPVADTLKRADGASAIDATVDRRGL
                     WRALTPQMFRYGALRAALQAAVAAGREPTDEAQCMEWQGARPLLVAGEDANIKITSPK
                     DLELAAAILATRAAAASPGEGMGARSCA"
     gene            1353..1820
                     /gene="ispF"
                     /locus_tag="VFA35_00715"
     CDS             1353..1820
                     /gene="ispF"
                     /locus_tag="VFA35_00715"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZT54717.1"
                     /translation="MHAFGPGDCLALGGVRVPHSHGVVSHSDGDVLLHALVDAMLGAG
                     GLGDIGEHFPDSDPRWRGAESRQFVSAAVEMLARRSWRVVNADLTLLAQQPRIAPWRE
                     QIRHSVAQMLQLTVADVNLKATTTEHLGFLGRNEGLAAMATVLIDRIPAGKGP"
     gene            complement(1817..>2212)
                     /locus_tag="VFA35_00720"
     CDS             complement(1817..>2212)
                     /locus_tag="VFA35_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZT54718.1"
                     /translation="RESLATPPAGVDHEPEDSLQYRRTGVPDSVLRRLRRGDLRIEAE
                     IDLHGLNRSQAEAQLREFLLEAVAARLQCLRIVHGKGLRSGSRGPVLREAALALLRRV
                     DRVLAYTPAAARDGGTGATLVLLRAATRT"
BASE COUNT          307 a          785 c          806 g          314 t
ORIGIN      
        1 aagaccggcg ccccgtcgcg gtccgatcgc gtggccaagt acaaccagct gctgcggatc
       61 gaggaggcgc tcggcgcgca ggcgaaatac gcggggcgcg atgccttccc gatcaggtta
      121 tgatcgcggc atgaggccag cgccatgaga ccagcgccat gaaatggttc gccgccgcgc
      181 tgctcgcggc actgctgatg ctccagtacc gcatctggtt ttccggggat ggcgtgcgcg
      241 aggtgctgcg gctgcgcacg gctgtcgcgg gccaacaggg agaaaatgag cgactgtcgg
      301 cccgcaatcg ccagcttgcg gccgaggtgc gcgacctcaa gcagggctac agcgccctcg
      361 aggagcgcgc gcgctccgac ctcggcatga tcggcgccga cgagaccttc ttccaggtgg
      421 ggccgggcga tgcgcccacg gccgcacccg gcaactcccc ggcgcccggc aatcccgctc
      481 cttccgccgt tcccacggcg ccccggacgc cgggcggcgg cccggcgacc gcgccggtcc
      541 tggcgccacc cgtgcgcacg gccgcggcgc gctagccgcc gcctcgccgc cggggccatg
      601 gtcgccgtgc gctactggct cgtgatgccg gcggccggtg cgagtcgccg cttcggtacc
      661 gcgcagtcca agctcgagcg ctcgcttgcg ggtcgcacgg tgctggatct cgcgctggaa
      721 ccgttcatgg acgacgagcg ttgccgcggc atcgcgctgg cgctcaatcc ggacggcctc
      781 gcgaatgcgg aagtccgcgc gcgcctcggg cgcaaggtgc tgccgatcgc cggtggcgag
      841 cggcgctgcg attcagtgct tgcgggcctg gcggcgcttt cgatggcgga agcggacgac
      901 tgggtgctcg tgcacgatgc ggccaggccc tgcctgagcg cggcggacct cgcggcattg
      961 ctggcagccg gagcgcagca cgccgtgggc gcactgcttg ccgagccggt cgccgatacg
     1021 ctcaagcgtg ccgacggcgc gtccgcgatc gacgccaccg ttgatcggcg cggcctgtgg
     1081 cgcgcgctga ctccgcagat gttccgttac ggcgcgttgc gcgcggcgct ccaggctgca
     1141 gtggccgccg ggcgcgagcc gacggatgaa gcccagtgca tggaatggca gggcgcccgg
     1201 ccgttgctgg tcgccggtga ggacgccaac atcaagatca cgagcccgaa ggatctcgaa
     1261 ctggccgcgg caatcctggc gacgcgcgcg gcggccgcct ctccgggcga gggcatggga
     1321 gcgcgctcgt gcgcgtagga tcgggcatcg acatgcacgc gtttggaccc ggagactgcc
     1381 tggcgctggg cggagtgcgc gtcccgcaca gccacggcgt ggtgtcgcac tccgacgggg
     1441 acgtgttgct gcatgcgctc gtcgatgcga tgctcggcgc cggcgggctg ggcgacatcg
     1501 gcgagcattt ccccgacagc gatccgcgct ggcgaggcgc cgagagccgg cagttcgtca
     1561 gcgccgcagt cgagatgctc gcacgacgtt cctggcgcgt cgtcaatgcc gacctcacgt
     1621 tgctcgcgca gcagccgcgc attgcgccgt ggcgggaaca gatccgtcat tccgtcgcgc
     1681 agatgctgca gctgacggtt gccgacgtga acctcaaggc aacgaccacc gagcaccttg
     1741 ggttcctggg ccgcaacgaa gggctggcgg ccatggcgac ggtcttgatc gaccgcatcc
     1801 ccgcaggcaa aggcccctag gtacgcgtcg ctgcgcgcag cagcaccagc gtggcgccgg
     1861 tgccgccatc gcgcgccgcc gccggtgtgt acgcgagtac ccgatccacc cggcgcagca
     1921 gtgccagggc cgcctcccgc agcaccggcc cgcgcgatcc ggagcgcagg cccttgccgt
     1981 gcacgatgcg caggcactgg agacgcgccg cgacggcctc cagcaggaat tcgcgcagct
     2041 gcgcctcggc ctgggaacgg ttgaggccgt gcaggtcgat ctccgcttcg atgcgcaggt
     2101 cgccgcgccg gaggcggcgc agcacggaat cgggcacacc cgtccgccga tactgcagcg
     2161 agtcttccgg ctcgtggtcg actcccgccg gcggtgtggc caggctctca cg
//
LOCUS       DASRDG010000047         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1273368, whole genome shotgun sequence.
ACCESSION   DASRDG010000047 DASRDG010000000
VERSION     DASRDG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273368"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="gspL"
                     /locus_tag="VFA35_00725"
     CDS             complement(<1..778)
                     /gene="gspL"
                     /locus_tag="VFA35_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HZT54719.1"
                     /translation="MSTLVVQIPPRARLHSGVDGVAAEASGLGTEYHYVSSPDGLALE
                     AQGHCAAALLPKATTVIAVLADTDVSWHRITLPKAPSARLRAALGGVLEDALLEDADD
                     VHLALEPEAVVGQPAWVAAASRRWLRAELATLEKADVFVDRVVPMSWPDDPPIGHFAE
                     TAVDPGSTAHGIALHWAHPDGVVSVRLQGGLARALVPAPAPAETRWSATPGAVAAAEQ
                     WLGGPVRVMAPGQRLLQAARSLWNLRQFDLARRNRGTRALR"
     gene            complement(892..1836)
                     /gene="gspK"
                     /locus_tag="VFA35_00730"
     CDS             complement(892..1836)
                     /gene="gspK"
                     /locus_tag="VFA35_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HZT54720.1"
                     /translation="MTTARPLRSARRQRGAALLTAMIIVTLVATLAAAMVWQQWRAVQ
                     VESAERARTQAAWMATAALDIARLYLREDQKSGLPTALTEPWAIPLEEARLSTLLAVD
                     KSNADDGPEAFLSGNITDLQARYNLTNLVVASKIVPAELQTLERLCQTLGVDGSVAAR
                     IAGGLRDAAAATGAVPLQPTTVSQLAWLGIDASSIASLRPYVAILPVVTPVNLNTASK
                     EVLAAAIEGLDTERLIQIRQRPPFRSPQDLVQRLSMPALPPTANVGVHSDFFEVRVRL
                     RIADRVLTERSVVQRQANPPRIVVLQRERIAGLEQLGS"
     gene            complement(1839..2507)
                     /locus_tag="VFA35_00735"
     CDS             complement(1839..2507)
                     /locus_tag="VFA35_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZT54721.1"
                     /translation="MKKPRATGAKGREGFTLVEVLVAMTVMAIMAVMAWQGVDGIVRA
                     RDASQVRLERTLRLNTVVAQWDQDLASIQDSSAVPQALACDGASVRMVRRTPEGLQIV
                     AWSLRPDASGGAWVRWAGPAVTTAGELQDSWLRSLQLQGGEPGALQALTGIAGWQVYF
                     YQGNAWANCQSSGNRVASIVMGGASAPPATRTALPSGVRAVLSFAAGSGLNGDLTRDT
                     LVGP"
     gene            complement(2504..2866)
                     /gene="gspI"
                     /locus_tag="VFA35_00740"
     CDS             complement(2504..2866)
                     /gene="gspI"
                     /locus_tag="VFA35_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HZT54722.1"
                     /translation="MNPRAARGFTLIEVLVALAIVAITLGAGIKAAGALTGNAQRLAD
                     TTAAQWCADNRLTALRLANLTPPVGDSEFECVQFGLTYRGTLSVRPSNQNNFVIVDAA
                     VADEAARPILHLTTLLRK"
     gene            complement(2863..>3287)
                     /locus_tag="VFA35_00745"
     CDS             complement(2863..>3287)
                     /locus_tag="VFA35_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspH"
                     /protein_id="HZT54723.1"
                     /translation="MVVVALIAIASALASLALRDPAATRLEHEGARLVALLEAARTEA
                     RAAGLTASWEPRAEQQGAEGFRFVGLPPGTEMPNRWLSAGVSAEVPGARAVKLGPEPL
                     IGAQRIVLHLDTQSLTLATDGLGPFVVVDPDPAAAPRS"
BASE COUNT          433 a         1169 c         1220 g          465 t
ORIGIN      
        1 cacgcagcgc gcgcgtgccg cggttgcgcc gcgccagatc gaactggcgc aggttccaca
       61 ggctgcgcgc ggcctgcagc aggcgctgcc ccggtgccat cacgcgcacc gggccgccca
      121 gccattgctc ggctgccgcg acggcgccgg gcgtggcgct ccagcgcgtc tcggccggcg
      181 ccggcgccgg caccagcgcg cgcgccaggc cgccctgcag gcgcacgctc acgaccccgt
      241 cggggtgcgc ccagtgcagc gcgatgccgt gcgccgtgct acccgggtcg accgcggtct
      301 cggcgaagtg gccgatcggc gggtcgtcgg gccaggacat cggcacgacg cggtcgacga
      361 acacgtcggc cttctcgagc gtcgcgagct cggcgcgcag ccagcggcgc gacgcggccg
      421 cgacccacgc gggctggccg accacggcct cgggctcgag cgcgaggtgc acgtcgtcgg
      481 cgtcttcgag caacgcgtct tcgagcacgc cgccgagcgc ggcgcgcagg cgtgcggacg
      541 gcgccttcgg cagcgtgatg cggtgccagc tgacatcggt gtcggcgagc accgcgatca
      601 cggtcgtcgc cttgggcagc agcgcggcgg cgcaatggcc ctgcgcttcg agtgcgaggc
      661 cgtcggggct gctgacgtag tggtattcgg tgcccagccc gctcgcctcg gccgcgacgc
      721 cgtcgacgcc gctgtgcagg cgcgcgcggg gcggaatctg gacaacgagg gtcgacatgg
      781 cgcgggcggg tgagttagcg gtgcattgta ggcacgcgtg gtttacaaat tgaagtgaat
      841 caaggactta ccgggcgttg ttgtagtcgc cgcgccgcag cgtgtggcag ctcagctgcc
      901 gagctgttcc aggccggcaa tgcgctcgcg ctgcagcacg acgatccgcg gcggattggc
      961 ctgtcgctgc accaccgaac gctcggtcag cacccggtcg gcgatgcgca ggcgcacacg
     1021 cacctcgaag aagtcggagt ggacgccgac gttcgcagtc ggtggcagcg ccggcatcga
     1081 caagcgctgc acgaggtctt gcggcgaccg gaacggcggg cgctggcgga tctggatcag
     1141 gcgctcggtg tccaggccct cgatcgccgc ggccagcacc tccttcgacg cggtgttcag
     1201 gttcaccggc gtgaccaccg gcaggatcgc gacgtagggc cgcaggctgg cgatcgaact
     1261 cgcatcgatg ccgagccagg ccagctggct cacggtcgtt ggctgcagcg gcacggcgcc
     1321 ggtcgcggcg gcggcatcgc gcaggccacc ggcgatccgc gctgcgacgc tgccgtcgac
     1381 gccgagcgtc tggcacaggc gctccagcgt ctgcaactcg gccgggacga tcttgctggc
     1441 cacgacgagg ttggtcaggt tgtagcgcgc ctgcaggtcg gtgatgttgc cagacagaaa
     1501 ggcttctggg ccgtcgtcgg cgttggactt gtcgaccgcg agcagggtcg acaggcgcgc
     1561 ttcttcgagc ggaatggccc agggttcggt cagcgctgtc ggcaggccac tcttctggtc
     1621 ctcgcgcagg tacaggcgcg cgatgtcgag cgcggcggtg gccatccagg cggcctgcgt
     1681 gcgcgcgcgt tcggccgact cgacttgcac cgcgcgccat tgctgccaga ccatcgcggc
     1741 ggcgagcgtc gcgaccagcg tgacgatgat catcgcggtc agcagcgccg cgccgcgttg
     1801 gcggcgtgcc gagcgaagcg gcctggcggt ggtcattctc aggggccgac gagcgtgtcg
     1861 cgcgtcaggt cgccgttcag cccgctgccg gcggcgaagc tcagcaccgc gcgcacgccg
     1921 ctgggcaagg cagtgcgcgt cgcgggcggc gcgctcgcgc cacccatcac gatcgacgcg
     1981 acccggttgc cgctcgactg gcagttggcc cacgcgttgc cctggtaaaa atagacctgc
     2041 catccggcaa tgccggtcag cgcctgcagc gccccgggct cgccgccttg cagttgcagg
     2101 ctgcgcagcc agctgtcttg caactcgccc gcggtcgtca cggccgggcc ggcccagcgc
     2161 acccaggcgc cgccgctggc gtcggggcgc agtgaccacg cgacgatctg caggccttcg
     2221 ggcgtgcggc gcaccatgcg cacgctcgcg ccgtcgcagg cgagcgcctg cggcacggcg
     2281 ctgctgtcct ggatcgacgc caggtcctgg tcccactgcg cgaccacggt gttcaggcgc
     2341 agtgtccgct cgagccgcac ctggcttgcg tcgcgcgcgc gcacgatgcc gtcgacgccc
     2401 tgccaggcca tcaccgccat gatcgccatc accgtcatcg cgaccagcac ctcgacgagc
     2461 gtgaatcctt cccgcccctt cgcccctgtc gcgcgcggct tcttcatttg cgcagcagcg
     2521 tggtgaggtg aaggatcggc cgcgcggcct cgtcggcaac cgcagcgtcg acgatcacga
     2581 agttgttctg gttcgacggc cgcaccgaca gcgtgccgcg gtaggtcagc ccgaactgga
     2641 cgcactcgaa ctcggagtcg ccgaccggcg gcgtgaggtt ggccaggcgc agcgcggtca
     2701 ggcggttgtc ggcgcaccac tgcgccgcgg tggtgtcggc caggcgctgg gcgttgccgg
     2761 tcaacgcgcc cgcggccttg atgccggcgc cgagcgtgat cgcgacgatc gccagtgcga
     2821 ccagcacctc gatcagcgtg aagccgcgcg cggcgcgtgg gttcatgacc tgggcgcggc
     2881 ggccgggtcg gggtcgacga cgacgaacgg gccgaggccg tcggtcgcga gcgtcaggct
     2941 ctgcgtgtcg aggtgcagca cgatgcgctg cgctccgatc agcggctctg gcccgagctt
     3001 gacggcgcgc gcgccgggca cctcggcgct gacaccggcg ctgagccagc ggttcggcat
     3061 ctcggtgccg ggcggcaggc cgacgaagcg aaagccctcg gcgccttgct gctcggcccg
     3121 gggctcccag ctcgccgtca ggccggccgc gcgggcctcg gtgcgggccg cttcgagcag
     3181 ggccacgagg cgcgcgccct cgtgctccag ccgggtcgcg gcagggtcgc gcaaggccag
     3241 gctggcgagc gcgctggcga tcgcgatcaa cgcgaccacg accatca
//
LOCUS       DASRDG010000048         5334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1511844, whole genome shotgun sequence.
ACCESSION   DASRDG010000048 DASRDG010000000
VERSION     DASRDG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511844"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFA35_00750"
     CDS             <1..332
                     /locus_tag="VFA35_00750"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HZT54724.1"
                     /translation="IHDPTQLNRQGPDRGTQYRSAVFTTSDAQKAAVERYIAQLDAAK
                     VYPAKIVTQVVPLAAFYPAEDYHQDYATLHPDFGYIATFDLPKIAALKTMFAAQYREQ
                     PVLVTKR"
     gene            384..1781
                     /locus_tag="VFA35_00755"
     CDS             384..1781
                     /locus_tag="VFA35_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZT54725.1"
                     /translation="MFPGLTLAYWLTVHGLVTCVAVLLYAITAHVLHQRRHPTAAIAW
                     VLFILLVPYLALPAFLAFGSRKQARPRNRPLLAPPATDAEGPWAIQTALALGQPPPAP
                     YTDLRIHADGAAALHALWQTIASAQHSIDLCTFIIGRDAVGYALIERLIAKARAGVRV
                     RVMLDGLGSLMRRRPSLAPLRAAGIEFTLFVPPLHSPLKGRINLRDHRKLLIADAGFA
                     TQRLWCGGRNLAGEYFEGDPGTPPWHDLSFDLRGAFVGQASTLFEHDWAFANGRKRHV
                     EIQGPRAETASRESAQMIASGPDQADDTVYALLVTAAYRARRRIALVTPYFVPDAALL
                     AALSLAARRGVQVDLLLPARSNHRMSDVARRRALRTLAAAGANVWVVPHMVHAKLVVI
                     DDTLALAGSANLDSRSLFLNYELMVAFHDREAIAGFASWFEHEMVGAQRLEPKPAGLF
                     TDIAEGLVLWMGFQL"
     gene            complement(1772..2773)
                     /locus_tag="VFA35_00760"
     CDS             complement(1772..2773)
                     /locus_tag="VFA35_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZT54726.1"
                     /translation="MDSLDLQVLSQARDWFNAGHKVWLVTVIETWGSAPRPPGALLCL
                     RDDGLVAGSVSGGCVEDDLIDRLRHGERVAAPSLIAYGVTKEEAARFGLPCGGNLRLV
                     QEPLRSSAWIDDILARTARHELVARRVALSTGEVSVEPAARGDAFAFDGERLVALFGP
                     RWRLLIIGAGQLSRAVAQMALTLDFEVICCDPRTEYNLGWDVPGTTFSDAMPDDLVLE
                     LQLDPHSAVVALTHDPKLDDLVLLEALKSPAFYIGALGSRGNTAKRRERLQMFDLTPA
                     EIDRLHGPIGLDIGAKTPAEIAVAIVAEIVAVKNGVLLTQKKVGSSMPPAARAQAQS"
     gene            complement(2788..3372)
                     /locus_tag="VFA35_00765"
     CDS             complement(2788..3372)
                     /locus_tag="VFA35_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HZT54727.1"
                     /translation="MELQGERTIPTSVDKTWAALNDPDVLKACIAGCETLERTGPETM
                     SAVVALKVGPVSARFKGNLLMTNIDAPNGYTINFDGQGGVAGFGKGSADVALTAQGEQ
                     QTLLKYAARATVGGKMAQIGSRLIDATAAKITEDFFKAFEAHLQAGVAPAAAGAQAAL
                     PATAAPAEPDSSVTKLAWLIGAVIVLAIVYFMIR"
     gene            complement(3380..4558)
                     /locus_tag="VFA35_00770"
     CDS             complement(3380..4558)
                     /locus_tag="VFA35_00770"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZT54728.1"
                     /translation="MIAENIVHFARVLRTAGMPIGPDRVLAAMAAVEQVGLSRRDDVH
                     AALGAVMLDRHEQQPLFDAAFDAFWRDPKLLEQLMLLLLPKISGRGDKVVPPRANRLA
                     EALAAPKPSAPPNPANRTAQEELTFDTTFTFSDRERLQRADFESMTTAEFELAKKLAE
                     QLPLPVQPVRRRRHERAARGTLDLRRTLLGMARQPHALLPAYTRPRRETPVLVVLLDI
                     SGSMERYARLLLHYVHGLTRRYLQVHTLTFGTRLTNITRSLRHRDPDVALAQADAQVH
                     DWKGGTRIASCLDEFNRVWARRLLGANAALLLMTDGLDRDEHGDLARAAAQLRRNAAQ
                     IVWLNPLLRFDGFEPRAAGVRALLPNVDRFLPVHNLASLADLGRALRAPAVAPSSLLA
                     "
     gene            complement(4699..>5334)
                     /locus_tag="VFA35_00775"
     CDS             complement(4699..>5334)
                     /locus_tag="VFA35_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZT54729.1"
                     /translation="AEAAHSIHGEGDRERLTHSLYSRSMLIERPLLQALTQTRSPVLL
                     IDELDRADEPFDAFLLEVLAENQLTIPEFGTVKADAPPITIITSNRTREIHDALKRRC
                     LYHWVDFPSVERELEIVRLKAPGAADRLYIQVVEFVQRMRRLDLFKAPGVAETIDWTN
                     ALIALNAIRLDPATVHDTLGVLLKYQDDIVKLQSGDTAKLLDELRARALLD"
BASE COUNT          760 a         1894 c         1854 g          826 t
ORIGIN      
        1 tgatccacga tccgacccag ctgaaccgcc agggcccgga ccgcggcacg caataccgct
       61 cggcggtgtt cacgaccagc gatgcgcaga aagcggcggt cgaacgctac atcgcgcagc
      121 tcgatgccgc caaggtctac ccggccaaga tcgtcacgca ggtcgtgccg ctcgccgcgt
      181 tctacccggc cgaggactac caccaggact acgcgacgct gcaccccgac ttcggctaca
      241 tcgcgacctt cgacctgccg aagatcgcgg ccctgaagac gatgttcgcg gcgcagtacc
      301 gcgagcagcc ggtgctggtg acgaaacgct gagcccgcgc cgcccgtggc cggcgccgcc
      361 gcggatgcgg taacgtcgcg ccgatgtttc ccggcctgac cctcgcctat tggctgaccg
      421 tccatggtct ggtgacctgc gtcgcggtgc tgctctacgc gatcaccgcg cacgtgctgc
      481 accagcgccg ccacccgacc gcggcgatcg cctgggtgct gttcatcctt ctggtgccgt
      541 acctcgcgct gccggccttc ctggcattcg gctcgcgcaa gcaggcgcgg ccgcgcaacc
      601 gcccgctgct cgcgccgccg gccaccgacg ccgaagggcc gtgggcgatc cagaccgcgc
      661 tcgcgctcgg ccagccgccg ccggcgccct acaccgacct gcgcatccac gccgacggcg
      721 ccgccgcgct gcacgccctg tggcagacga tcgcctcggc gcagcactcg atcgacctgt
      781 gcaccttcat catcggccgc gacgcggtcg gctacgcgct gatcgagcgc ctgatcgcga
      841 aggcacgcgc cggcgtgcgg gtgcgcgtga tgctcgacgg cctgggcagc ctgatgcgcc
      901 ggcgcccgag cctcgcgccg ctgcgcgccg cgggcatcga gttcacgctg ttcgtgccgc
      961 cgctgcactc gccgctgaag ggccgcatca acctgcgcga tcaccgcaag ctgctcatcg
     1021 ccgacgccgg cttcgcgacc cagcgcttgt ggtgcggcgg gcgcaacctc gcgggcgaat
     1081 acttcgaggg cgaccccggc acgccgccgt ggcacgacct gagcttcgac ctgcgcggcg
     1141 cgttcgtggg ccaggcgagc acgctgttcg agcacgactg ggcgttcgcg aacgggcgca
     1201 agcgacatgt cgagatccag ggcccgcgtg ccgaaacggc gagccgcgag agcgcgcaga
     1261 tgatcgcctc cggccccgac caggccgacg acacggtgta cgcgctgctc gtcaccgcgg
     1321 cctaccgcgc gcgccgccgc atcgcgctcg tcacgccgta cttcgtgccc gacgcggcgc
     1381 tgttggccgc gctgagcctg gcggcgcggc gcggcgtgca ggtcgatctg ctgctgccgg
     1441 cgcgctccaa ccaccgcatg tcggacgtgg cgcgccgccg cgcgttgcgc acgctcgcgg
     1501 cggccggcgc gaacgtctgg gtcgtgccgc acatggtcca cgccaagctc gtcgtgatcg
     1561 acgacacgct cgcgctcgcc ggctcggcca acctcgacag ccgcagcctg ttcctgaact
     1621 acgagttgat ggtcgcgttc cacgaccgcg aggcgattgc cggcttcgcg tcgtggttcg
     1681 agcacgagat ggtcggtgcg cagcgcctgg agcccaagcc cgccgggctg ttcaccgaca
     1741 tcgccgaggg gctggtgctg tggatgggct ttcagctctg agcttgcgcc cgcgcagccg
     1801 gcggcatcga cgagccgacc ttcttctgcg tcagcagcac gccgttcttc accgccacga
     1861 tctcggcgac gatcgccacc gcgatctcgg ccggcgtttt cgcaccgatg tccagcccga
     1921 tcgggccatg caggcggtcg atctcggccg gcgtgaggtc gaacatctgc aggcgctcgc
     1981 gccgcttcgc ggtgttgccg cgcgagccga gtgcgccgat gtagaaggcc ggcgacttca
     2041 gcgcttcgag cagcaccagg tcgtcgagct tcgggtcgtg cgtgagcgcg acgaccgcgc
     2101 tgtgcgggtc gagctgcagt tcgagcacca ggtcgtcggg catcgcgtcg ctgaaggtag
     2161 tgccgggcac atcccagccc aggttgtact cggtgcgcgg gtcgcagcag atcacctcga
     2221 agtcgagcgt gagcgccatc tgcgcgaccg cgcgcgagag ctgccccgcg ccgatgatca
     2281 gcagccgcca gcgcgggccg aacagcgcga cgagccgctc gccgtcgaac gcgaacgcgt
     2341 cgccgcgcgc ggccggctcg acgctgacct cgccggtcga cagcgccacg cgccgcgcga
     2401 cgagctcgtg ccgcgcggtg cgcgcgagga tgtcgtcgat ccacgcgctg ctccgcagcg
     2461 gctcctggac caggcgcagg ttgccgccgc acggcaggcc gaagcgcgcg gcctcctcct
     2521 tggtcacgcc ataggcgatc agcgacggcg cggcgacgcg ctcgccatgg cgcaaccggt
     2581 cgatcaaatc gtcttcgaca caaccgcccg agaccgagcc ggccaccagc ccgtcgtcgc
     2641 gcaggcacag caacgcaccg ggcgggcgag gcgcgctgcc ccaggtttcg atcacggtca
     2701 cgagccagac cttgtggccg gcattgaacc agtcgcgcgc ctgactcagt acctgcaggt
     2761 cgaggctgtc catcgaagca ctcccagcta gcggatcatg aagtagacga tcgcgagcac
     2821 gatcaccgcc ccgatcagcc acgcgagctt ggtgacgctg ctgtcgggtt cggccggcgc
     2881 ggccgtggcc ggcagcgccg cctgcgcgcc ggctgcggcc ggcgcgacgc cggcctgcag
     2941 gtgcgcctcg aaggccttga agaagtcttc ggtgatcttg gccgcggtcg cgtcgatcag
     3001 gcgcgagccg atctgcgcca tcttgccgcc gacggtcgcg cgtgcggcgt acttcagcag
     3061 cgtctgctgc tcgccctgcg cggtcagcgc cacgtcggcc gagcccttgc cgaagccggc
     3121 gacgccgccc tgcccgtcga agttgatcgt gtagccgttc ggcgcatcga tgttggtcat
     3181 caagaggttg cccttgaagc gcgcgctgac cgggccgacc ttcagcgcga ccaccgcgct
     3241 catcgtctcg gggccggtgc gctccagcgt ctcgcagccg gcgatgcagg ccttcagcac
     3301 atcgggatcg ttgagcgcgg cccaggtctt gtcgaccgaa gtcgggatgg ttcgttcgcc
     3361 ttgcagttcc atggtgttct tatgccagaa gtgacgaagg ggcgacagcc ggcgcccgca
     3421 aggccctgcc gagatcggcc aggctcgcga gattgtgaac cggcaggaag cggtcgacgt
     3481 tcggcagcag cgcgcgcaca ccggcggcgc gcggttcgaa gccgtcgaag cgcagcagcg
     3541 ggttgagcca gacgatctgg gcggcattgc ggcgcagctg cgccgcggcg cgggcgaggt
     3601 cgccgtgctc gtcgcggtcc agcccgtcgg tcatcagcag cagcgcggcg ttggcgccga
     3661 gcaggcgccg cgcccagacg cggttgaact cgtccaggca actcgcgatg cgcgtgccgc
     3721 ccttccagtc gtgcacctgc gcatcggcct gcgccagcgc gacatcgggg tcgcggtgcc
     3781 gcaggctgcg cgtgatgttg gtcagccgcg tgccgaaggt cagcgtgtgc acctgcaggt
     3841 agcggcgcgt cagcccgtgc acgtagtgca gcagcaggcg cgcgtagcgc tccatcgagc
     3901 cggagatgtc gagcagcacg accagcaccg gcgtctcgcg gcgcggccgg gtgtaggccg
     3961 gcagcagcgc gtgcggctgg cgtgccatgc cgagcagcgt gcggcgcagg tccagcgtcc
     4021 ctcgcgccgc gcgctcgtgg cggcgccggc gcaccggctg caccggcagc ggcagctgtt
     4081 cggcgagctt cttcgcgagc tcgaactcgg ccgtcgtcat gctctcgaag tcggcccgct
     4141 gcaggcgctc gcggtcggag aacgtgaacg tcgtgtcgaa ggtcagttct tcctgcgcgg
     4201 tgcggttcgc cgggttcggc ggcgccgacg gcttcggcgc ggccaacgcc tcggcgagcc
     4261 ggttcgcgcg cggcggcacc accttgtcgc cgcggccgct gatcttgggc agcagcaaca
     4321 gcatcagttg ctcgagcagc ttcggatcgc gccagaacgc gtcgaacgcg gcgtcgaaca
     4381 gcggctgctg ttcgtggcgg tcgagcatca cggcgccgag tgcggcatgc acgtcgtcgc
     4441 gccggctcag cccgacctgc tcgaccgcgg ccatcgccgc gagcacgcga tccgggccga
     4501 tgggcatgcc ggcggtgcgc agcacgcgcg cgaagtggac gatgttctcg gcaatcatgt
     4561 tcgaacgtcg aagagagaac gcaaagaacg caaaggttcg ccaaggacgc caaggaaaga
     4621 caaggcattc attcttctgc tttttctttg cgacctttgc gtcccttggc gtctttgcgg
     4681 tccgtattga ttcccctctc aatccagcaa cgcccgcgcc cgcagttcat cgagcagttt
     4741 cgcggtgtcg ccgctttgca gcttgacgat gtcgtcctgg tacttcagca gcacgccgag
     4801 cgtgtcgtgc accgtggccg ggtcgaggcg gatcgcgttg agcgcgatca gcgcgttggt
     4861 ccagtcgatc gtctcggcga cgccgggtgc cttgaacagg tcgagccggc gcatgcgctg
     4921 cacgaactcg acgacctgga tgtacaggcg atcggccgcg ccgggcgcct tcaggcgcac
     4981 gatctcgagc tcgcgctcga cgctcggaaa gtcgacccag tggtacaggc agcgccgctt
     5041 cagcgcatcg tggatctcgc gcgtgcggtt gctggtgatg atcgtgatcg gcggggcgtc
     5101 ggccttgacg gtgccgaact cgggaatcgt cagctggttc tcggccagca cttcgagcag
     5161 gaaggcatcg aagggctcgt cggcgcggtc gagctcgtcg atcagcagca ccggcgagcg
     5221 cgtttgcgtc agcgcctgca gcagcgggcg ctcgatcagc atgctgcgcg agtacaggct
     5281 gtgcgtgagg cgctcgcggt cgccttcgcc gtggatcgag tgcgcggctt cggc
//
LOCUS       DASRDG010000049         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_468552, whole genome shotgun sequence.
ACCESSION   DASRDG010000049 DASRDG010000000
VERSION     DASRDG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468552"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VFA35_00780"
     CDS             complement(<1..1325)
                     /locus_tag="VFA35_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54730.1"
                     /translation="MFAALGVFSGSFALDSAQALVAGPEMDAWAVLEHLGALVDKSLV
                     AVEGSAADLPRYRLLETTRAFALERLAASGATPRILRRHAEVTLEIFEHAHSDLMRGM
                     PSADLVPRLTPDLDNLRGALRWAAGADGDDRLAVALFGAAIEGQGHFYFFALGAETWR
                     WRQVLRSRVAASMPGAIAARFWLACAQWGSILSPKEAADDARRAIALYTALGDRFGTF
                     RSWQALAYAHSSAGRHDEALEALRPAIELRDPTWPQWILAIFDNTAGIVCSQAGDLAR
                     ARRHYTAFFEVCGRIGPVDELNATALLVDLDVAEGLVQKAGEQAAAMIARPGAMTLQW
                     SDGRGLRMYATALMVSGHLDDAERVYRQSLGELRRYYGNGAATLLDAATWLARKERLE
                     DAARVLAYAEAVHESEGRSPRLVARQLRDRLHAELAQRFAADRLARLYE"
     gene            1353..>2595
                     /locus_tag="VFA35_00785"
     CDS             1353..>2595
                     /locus_tag="VFA35_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54731.1"
                     /translation="MAPFERGAQGLVPAQGDACASGQQAKALVEALPHAVDAEQGHAP
                     GAELDRERNAVEPAADLDHAGDVLRRERKAVDERACAGNEELHGTGGCGRVEAGCISP
                     VARNGQRAEPVHVLVGMAQQLLARRQHVDAGRAAAQQRHEGVDGLEHVLAVVEDQQQA
                     LCGERLQRMLEARRRIADRDAERTRDRGRDQRRLDQRRELDQAHAVRERADVLAGRDR
                     GEPGLADPRGADDRDEAALANALAECVDIVLPAEQLFDLGGRHCRSDAFARRGPVGLG
                     RCRTLARHREAIAPPGNGGDRLRPEQLAQGGDLHLQVVFLDDEARPRQIEELLLGDQL
                     AGAFGERHEHVERACADRDRSAADNEAALHGLQHEGIEAEVVGGHARSGASRRQRVCA
                     IGTGCSNCQAPAGLARAPRDDG"
BASE COUNT          402 a          934 c          924 g          335 t
ORIGIN      
        1 tcctcgtaca ggcgtgcgag cctgtctgcc gcgaaacgct gcgcgagctc ggcgtgcagg
       61 cggtcgcgca actggcgggc cacgagacgc ggcgaacggc cctcgctctc atgcacggct
      121 tcggcatagg cgagaacgcg cgcggcgtct tcaagccgct ccttgcgcgc gagccacgtg
      181 gcagcgtcga gcaaggtcgc cgccccgttt ccatagtagc ggcggagctc gccgagcgac
      241 tggcgataaa cccgctcggc atcgtcgaga tggcccgaca ccatgagcgc cgtcgcgtac
      301 atgcgcaagc cccggccatc gctccattgc agcgtcatcg ccccgggccg cgcgatcatg
      361 gcagcggcct gctcgccggc cttctgtact agtccttccg cgacgtcaag gtcgaccagc
      421 aaggccgtcg cgttcagctc gtcgacggga ccgatgcggc cgcagacctc gaagaacgcc
      481 gtgtagtgcc ggcgcgcgcg agccagatcg ccagcctgcg aacagacgat gcccgctgtg
      541 ttgtcgaaga tcgcaaggat ccactgcggc caagtcgggt cgcgcagctc gatcgccggg
      601 cgcaacgcct cgagcgcttc gtcgtggcgg cccgccgacg agtgcgcgta tgcgagcgcc
      661 tgccagctac ggaaggtccc gaaccggtcg ccgagcgccg tgtagagcgc gattgcgcgc
      721 cgtgcatcgt cggccgcttc cttcggcgaa agtatgctgc cccactgcgc gcaagcgagc
      781 cagaaacgcg cggcgatggc gccgggcatc gaggcggcca cccgggaccg cagcacctgg
      841 cgccagcgcc acgtctcggc gccgagcgcg aagaaataaa agtgcccctg gccctcgatg
      901 gcggccccga acagcgccac ggcgaggcga tcgtcaccgt ccgcaccggc tgcccagcgc
      961 aacgcgccgc gcaggttgtc aaggtcgggc gtaaggcgcg ggaccaagtc ggccgacggc
     1021 atgccacgca tcaagtcgct gtgcgcatgc tcaaagatct cgagcgtcac ttcggcgtgc
     1081 cggcgcaaga tgcgcggcgt cgctccgctt gccgcgagac gctcgagcgc gaacgcgcgc
     1141 gtggtctcga gcagccggta gcgcggcaag tcggccgcgc tgccttcgac cgcgacgagc
     1201 gacttgtcga ccagcgcgcc caggtgctcg agcacggccc acgcatccat ctccggcccg
     1261 gccaccagcg cctgcgccga gtcgagcgcg aagcttcccg agaacacgcc gagcgcggcg
     1321 aacaccgtct gctcgggcgc ggtcagcagg ccgtggctcc attcgagcgc ggcgcgcagg
     1381 gtctggtgcc ggcgcaaggc gacgcgtgcg cctccggtca gcaggcgaaa gcgctcgtcg
     1441 aggcgctgcc gcacgccgtc gacgccgagc aagggcacgc gcccggcgcc gagctcgatc
     1501 gcgagcggaa tgccgtcgag ccggcggcag atctcgacca cgccggcgac gttctgcgcc
     1561 gtgagcgcaa agccgttgac gagcgagcgt gcgcgggcaa cgaagagctg cacggcaccg
     1621 gcggctgcgg ccgcgtcgag gccggctgca tcagccccgt cgcccggaac ggacagcggg
     1681 ccgagccggt acacgtgctc gtcgggatgg cgcagcagct cctggctcgt cgccagcacg
     1741 tggatgccgg gcgcgctgcg gcgcagcagc gtcacgaagg cgtcgacggc ctcgagcacg
     1801 tgctcgcagt tgtcgaggac cagcagcagg cgctgtgcgg cgagcgcctg cagcgcatgc
     1861 tcgaggctcg acggcgcatc gccgatcgcg acgccgagcg cacgcgcgat cgtgggcgcg
     1921 accagcgtcg cctcgaccag cggcgcgagc tcgaccaggc gcatgccgtc cgcgaacgcg
     1981 ccgacgtgct cgcgggccgc gaccgcggcg agccgggtct tgccgatccc cgcggggccg
     2041 acgaccgtga cgaggccgcg ctcgcgaatg ctcttgccga gtgcgtcgat atcgtcctgc
     2101 cggccgaaca gctcttcgac ctcggtggtc ggcactgccg cagcgacgcg ttcgcccggc
     2161 gaggccccgt cggtctcgga cgctgccgca cccttgcgcg ccatcgcgag gcgatagccc
     2221 cgcccgggaa tggtggcgat cgcctgcggc ccgagcagct tgcgcaaggc ggtgatctgc
     2281 acctgcaagt tgttttcctc gacgacgagg cccggccacg ccaaatcgag gagctcctgc
     2341 ttggagacca gctggccggc gcgttcggcg agcgccacga gcacgtcgaa cgcgcgtgcg
     2401 ccgatcgcga ccggagcgcc gccgacaatg aggcggcgct ccacggcctg cagcacgaag
     2461 gtatcgaagc agaggtcgtc ggtggtcacg cgcgttcggg agcgtctcgt cggcagcgag
     2521 tatgcgccat cgggacgggt tgctcgaact gtcaggcccc tgccggattg gcgcgggccc
     2581 cccgcgacga cggag
//
LOCUS       DASRDG010000050         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_361012, whole genome shotgun sequence.
ACCESSION   DASRDG010000050 DASRDG010000000
VERSION     DASRDG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361012"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFA35_00790"
     CDS             complement(<1..599)
                     /locus_tag="VFA35_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HZT54732.1"
                     /translation="MNSIRVRVLLSLLGMLVVTALVMGALTYRSELAETESLFDYQLR
                     QMAFSLRDQGEIAPAQASTLADDELDFVIQIWTVDGRVIYPPRVRDDLPTRALLGLTD
                     VTVGGVTWRTFSVASRDRVIQVAQPLQTRRRLAAGAALRSVAPLLIVAPAMALLVWWL
                     AARTLAPLQRLAAGVRARDEQSLQPLPATGLPDEVAPLVA"
     gene            complement(596..1297)
                     /locus_tag="VFA35_00795"
     CDS             complement(596..1297)
                     /locus_tag="VFA35_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZT54733.1"
                     /translation="MRLLLVEDDRMIGESLRGALRLEGYAVDWLRDVAAAHATLASER
                     FDLVLLDLGLPASGAPGAPTRAGGGLDVLRALRARQDATPVIVLTARDGPGDRVAGLD
                     AGADDYLVKPFELDELAARIRAVLRRHSGRAQPTLACGGVTLDPATRQVSRDGAPVLL
                     SAREFAVLEALMLRPGALLSRAQLEDRLYGWGEEIESNAVSVYIHQLRRKLGADFIQN
                     VRGVGYFVGDKPAGR"
     gene            1437..2810
                     /locus_tag="VFA35_00800"
     CDS             1437..2810
                     /locus_tag="VFA35_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZT54734.1"
                     /translation="MPTILRHTLLSFRDLLATFGPFIVLTCGLLALAYWVLDPTPPKK
                     VVLATGTDQGAYAEFGKRYAEILKGYGITVELRATEGAAENLRLLRDPNSGVDIAFVQ
                     GGAGERARKQADSVSRADDDDEAGLVSLGSLFVEPVWLFYRTDSAERLLKAPALTSLS
                     QLAGWRINIGARGSGAPNLMFKLLEANRIDPSMLTIVRKSQTPATVDLIAGEVDALVF
                     ASAPESLMVQMLLRTPGIALYDFAQADAYSRRFPFLSPVTLPRGVVDLAGDVPPRDVR
                     LIAPTATLVARAQTHPALIQLFVQAAQQIHGGAGWFQRKGDFPKADGTEQPLAKEAQR
                     FYASGTPVLQRYLPFWLANLIERMWPVLVTIIAVLIPLSRMLPPLYQFKIRSRIFRWY
                     GQLREVEDAIGKRPAEELLTELKRIESRVEGVNVPLSYADELYSLRSHIQMVGNRLRS
                     APTEASA"
     gene            2807..4183
                     /locus_tag="VFA35_00805"
     CDS             2807..4183
                     /locus_tag="VFA35_00805"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZT54735.1"
                     /translation="MNRKAMEENKPKVIIIGCGFGGIEAVRMLSKAPVEITMIDRTNH
                     HLFQPLLYQVATAGLSAPAISVPIRYMLRREMKRGNLTILQGEVVAIDAAAKRVLLDG
                     GQSLPYDDLIVAAGATHSYFGHDDWAQFAPGLKTLDEAFKIRARVIGAFERAERLDDA
                     AARAPWLTFVVIGAGPTGVEMAGTMIEIARHTLRQEFRRIDSTRARVVLLEGSDRVLG
                     AFVPQLSQRAREQLDKLGVDVRTGCQVTGIDADGVDYETREGDAVKRYRLPSKTVIWA
                     AGVAGSALGRSLAASAGASLDRAGRVIVRPDLSLTDHPEITVIGDLASAMSYPADPGG
                     KPTPVPGVSPGAKQMGRTAAANLLRRLKGEATEPFRYVDYGNLATVGRKAAVVDLTVP
                     RIGALRFSGFFAWLFWLFAHIWFLIGFRNRFVVMLDWAWAYWTYERLARVVAEPPSTS
                     HAPAAPPG"
     gene            complement(4111..>4343)
                     /locus_tag="VFA35_00810"
     CDS             complement(4111..>4343)
                     /locus_tag="VFA35_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54736.1"
                     /translation="MCRFLRGYLDGLIVRSTFLAVLAIGLAPLGGWLAFPLAMLVAAG
                     VLLAVTALRQPGGAAGACEVEGGSATTRARRS"
BASE COUNT          651 a         1561 c         1526 g          605 t
ORIGIN      
        1 gcgaccagcg gcgccacctc gtcgggcagg ccggtggcgg gcagcggctg cagcgactgc
       61 tcgtcgcgcg cgcgcacgcc cgcggcgagg cgctgcagcg gcgcgagcgt gcgcgcggcc
      121 agccaccaga ccagcagtgc catcgccggc gcgacgatca gcagcggcgc gacgctgcgc
      181 agcgccgccc cggcggcgag gcggcgccgc gtctgcagcg gctgcgcgac ctggatcacc
      241 cggtcgcgcg acgccacgct gaaagtacgc caggtcacgc cgcccacggt cacgtcggtc
      301 aggccgagca gggcgcgcgt cggcaggtcg tcgcgcacgc gcggcgggta gatcacgcgg
      361 ccatcgaccg tccagatctg gatgacgaag tcgagctcat cgtcggccag cgtgctcgcc
      421 tgcgccggcg cgatctcgcc ctggtcgcgc agcgagaacg ccatctggcg cagctggtag
      481 tcgaacagcg attcggtctc ggcgagctcg ctgcggtagg tcagcgcacc catcaccagc
      541 gcggtgacga cgagcatgcc gagcagcgac agcagcacgc ggacgcggat cgaattcatc
      601 ggcctgcggg cttgtcgcca acgaaatagc cgacgccgcg gacgttctgg atgaagtcgg
      661 cgccgagctt gcgccgcagc tggtggatgt agaccgagac cgcgttgctc tcgatctcct
      721 cgccccagcc gtagagccgg tcttcgagct gcgcgcgcga cagcagcgcg ccgggccgca
      781 gcatcagggc ctcgagcacc gcgaactcgc gcgccgagag cagcaccggc gcgccgtcgc
      841 ggctgacctg gcgcgtcgcc gggtcgagcg tgacgccgcc gcacgcgagc gtcggttgcg
      901 cgcgcccgct gtggcggcgc agcacggcgc ggattcgcgc ggcgagttcg tcgagttcga
      961 agggcttgac gaggtagtcg tcggcgccgg catcgaggcc ggcgacccgg tcgcccgggc
     1021 cgtcgcgtgc ggtcagcacg atcaccgggg tcgcgtcctg gcgcgcgcgc agcgcgcgca
     1081 gcacgtcgag cccgccgccg gcgcgggtcg gcgcaccggg tgcgccgctg gccggcaggc
     1141 ccaggtcgag cagcacgagg tcgaagcgct cgctggcgag ggtcgcgtgc gccgcggcca
     1201 cgtcgcgcaa ccagtcgacc gcgtagccct cgagccgcag cgcgccgcgc aggctctcgc
     1261 cgatcatcct gtcgtcttcc accagcagca gtcgcatcct gaaaaggata ccttgcgctt
     1321 cggttgcacc gcgggccgcc gcgccgggcc gggccgcccc aagcagggcc cgacccgccc
     1381 ggagggtcga ccgccgtacc cggcgggcgg ggtgcggaca agttatcgtc gcggccatgc
     1441 cgacgatcct gcgccacacg ctgctctcgt ttcgcgacct gctcgcgacc ttcggcccgt
     1501 tcatcgtgct gacgtgcggg ttgctggcgc tggcctactg ggtgcttgac ccgacgccac
     1561 cgaagaaggt cgtgctcgcg accggcaccg accagggcgc ctacgccgag ttcggcaagc
     1621 gctacgccga gatcctgaag ggctacggca tcacggtcga gttgcgcgcg accgagggcg
     1681 cggccgagaa cctgcggctg ctgcgcgacc cgaactcggg cgtcgacatc gcgttcgtgc
     1741 agggcggcgc cggcgagcgc gcgcgcaagc aggccgattc ggtctcgcgc gccgacgacg
     1801 atgacgaagc cggcctggtc tcgctcggta gcctgttcgt cgagccggtg tggctgttct
     1861 atcgcaccga ctcggccgag cggctgctga aggcgccggc gttgaccagc ctgtcgcagc
     1921 tggccggctg gcgcatcaac atcggcgcgc gcggcagcgg cgcgccgaac ctgatgttca
     1981 agctgctcga ggcgaaccgg atcgacccga gcatgctgac gatcgtgcgc aagtcgcaga
     2041 cgcctgcgac cgtcgacctg atcgcgggcg aagtcgacgc gctggtgttc gcctcggcgc
     2101 ccgagtcgct gatggtgcag atgctgctgc gcacgcccgg catcgcgctc tacgacttcg
     2161 cgcaggccga tgcgtactca cgccgcttcc cgttcctgag cccggtgacc ctgccacgcg
     2221 gcgtggtcga tctggctggc gatgtgccgc cgcgcgacgt gcgcctgatc gcaccgaccg
     2281 ccacgctggt tgcgcgcgcc cagacgcacc cggcgctgat ccagctcttc gtgcaggcgg
     2341 cgcagcagat ccacggcggc gccggctggt ttcagcgcaa gggcgacttc ccgaaggccg
     2401 acggcaccga gcagccgctc gccaaagagg cgcagcgttt ctacgccagc ggcacgcctg
     2461 tgctgcagcg ctacctgccg ttctggctcg cgaacctgat cgagcgcatg tggccggtgc
     2521 tggtgaccat catcgcggtg ctgatcccgc tgtcgcggat gctgccgccg ctgtaccagt
     2581 tcaagatccg ctcgcgcatc tttcgctggt atggtcagtt gcgcgaggtc gaagacgcga
     2641 tcggcaagcg gccggcagaa gaattgctta ccgagttgaa gcgcatcgaa tcgcgcgtcg
     2701 aaggcgtgaa cgtgccgctg agctacgccg acgagctcta ctcgctgcgc agccacatcc
     2761 agatggtcgg caaccggctg cgatccgcgc cgaccgaggc aagcgcatga acaggaaggc
     2821 aatggaagag aacaaaccca aggtcatcat catcggctgc ggcttcggcg gcatcgaggc
     2881 ggtgcgcatg ctctcgaagg cgccggtcga gatcacgatg atcgaccgca ccaatcacca
     2941 tctgttccag ccactgctgt accaggtcgc gaccgccggg ctgagcgcgc cggcgatcag
     3001 cgtgccgatc cgctacatgc tgcggcgcga gatgaagcgc ggcaacctga cgatcctgca
     3061 gggcgaggtc gtcgccatcg acgccgcggc gaagcgagtg ctgctcgacg gcggccagtc
     3121 gctgccctac gacgacctga tcgtcgccgc cggtgcgacc cacagctact tcggccacga
     3181 cgactgggca cagttcgcgc cggggctgaa gacgctcgac gaagcattca agatccgcgc
     3241 ccgcgtgatt ggcgcgttcg agcgcgccga gcggctcgac gatgcggccg cacgcgcgcc
     3301 atggctgacc ttcgtcgtga tcggcgccgg tccgaccggc gtcgagatgg ccggcacgat
     3361 gatcgaaatc gcgcggcaca cgctgcggca ggagttccgg cgcatcgact cgacgcgcgc
     3421 ccgcgtcgtg ctgctcgaag gctccgaccg cgtgctcggc gccttcgtgc cgcagctctc
     3481 gcagcgcgcg cgcgagcaac tcgacaaact cggcgtcgac gtgcgcaccg gctgccaggt
     3541 gaccggcatc gatgccgatg gcgtcgacta cgaaacccgc gagggcgatg ccgtcaagcg
     3601 ctaccggttg ccgagcaaga ccgtgatctg ggccgccggc gtcgcgggtt cggcgctggg
     3661 ccggtcgctc gcggcaagcg ccggcgccag cctcgaccgc gccggccgcg tcatcgtccg
     3721 ccccgacctg agcctgaccg accaccccga gatcaccgtg atcggcgacc tggcctcggc
     3781 gatgagctat cccgcagacc ccggcggcaa gccgacgccg gtgcccggcg tcagccccgg
     3841 cgcgaaacag atgggccgca ccgccgccgc gaacctgctg cggcggctga agggcgaggc
     3901 gaccgagccg tttcgctacg tcgactacgg caacctcgcg accgttggcc gcaaggcagc
     3961 ggttgtcgac ctgacggtgc cgcgcatcgg cgcgctgcgc ttttccggct tcttcgcctg
     4021 gctgttctgg ctgttcgcgc acatctggtt cctgatcggc tttcgcaacc gcttcgtcgt
     4081 gatgctcgac tgggcctggg cgtactggac ctacgagcgt ctggcccggg tcgtggccga
     4141 gccgccctcg acctcgcacg cgccggccgc gccgccgggc tgacgcagcg cggtcacggc
     4201 cagcagcacg cccgccgcca ccagcatcgc cagcgggaac gccaaccagc cgccgagtgg
     4261 cgccaggccg atcgccagca cggcaaggaa ggtactgcgc acgatcaggc cgtcgaggta
     4321 gccgcgcaga aagcggcaca tcg
//
LOCUS       DASRDG010000051         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_361323, whole genome shotgun sequence.
ACCESSION   DASRDG010000051 DASRDG010000000
VERSION     DASRDG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361323"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFA35_00815"
     CDS             <1..315
                     /locus_tag="VFA35_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54737.1"
                     /translation="QSTRPRTGTATTTTATTASGVKVDPVAQRARDSDARRILEGELK
                     NEETKLAAMEKEFNGGQPERLGDEKNYQKYLDRVADMKTAIARKEGDIAALKREIAKQ
                     PQ"
     gene            357..1430
                     /gene="glnL"
                     /locus_tag="VFA35_00820"
     CDS             357..1430
                     /gene="glnL"
                     /locus_tag="VFA35_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HZT54738.1"
                     /translation="MPAGALAYAAFDHLATLVAVVGIDGQCVFANAAFENVLGLSRRS
                     VQRGSVFDWFVDPQRLRDTVVAVSRNEYATSRLEALLRRPGALHGEPLPVHVIVNQME
                     ASPHVVVELVEIEQQTRQDREERALDQAQVTKELIRNLAHEIKNPLGGIRGAAQLLEM
                     EVQERALHEYTQVIIGEADRLQALVDRLLAPHRKPHVVSDVNIHEVCERVRALIQAEF
                     PRGLAIERDYDISIPDFRGDREQLIQAVLNIAHNAAQALAERITTGGDARITLRTRIA
                     RQVTLGKQRYRLALDLHIEDNGPGVPESIRDRIFYPLVSGRDGGSGLGLTLAQTFVQQ
                     HLGTIECESEPGRTTFKIVIPLP"
     gene            1501..>2216
                     /locus_tag="VFA35_00825"
     CDS             1501..>2216
                     /locus_tag="VFA35_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZT54739.1"
                     /translation="MKPIWIVDDDQSIRFVLEKALAREDFAVRSFTNSRDVLKALDDD
                     EPQVLVSDIRMPGGSGIELLAKVRERMPALPVIIMTAYSDLDSAVSAFQGGAFEYLPK
                     PFDVPKAVELIRRAVDESLREEVRDEKAAQMPEMLGQAPAMQDVFRAIGRLSQSIVTV
                     LITGESGSGKELVARALHKHSPRAQGPFVAINTAAIPKDLLESELFGHERGAFTGATA
                     TKPGLFEMAHGGTLLLDEVG"
BASE COUNT          391 a          765 c          763 g          297 t
ORIGIN      
        1 cagtcgacgc gcccgcgcac gggcacggcg acgacgacga ccgcgacgac ggcgtcggga
       61 gtcaaggtcg atccggtcgc gcagcgcgcc cgcgacagcg acgcccgccg catcctcgag
      121 ggcgagctca agaacgagga aacgaagctc gcggcgatgg agaaggaatt caacggcggc
      181 cagcccgagc gtctcggcga cgagaagaac taccagaagt acctcgaccg ggtcgccgac
      241 atgaagaccg cgatcgcgcg caaggagggc gacatcgccg cgctcaagcg cgagatcgcc
      301 aagcagccgc agtgaggcag cctgctgcgc gaccggcagc ttccggcacg cccgccgtgc
      361 ccgccggtgc gctggcttac gcggctttcg accacctcgc cacgctggtc gcggtcgtcg
      421 gcatcgacgg ccagtgcgtg ttcgcgaatg ccgcgttcga gaacgtgctc ggtctgtcgc
      481 ggcgcagcgt gcagcgcggt tcggtgttcg actggttcgt cgacccgcag cggctgcgcg
      541 acaccgtcgt cgcggtcagt cgcaacgagt acgccaccag ccgcctcgaa gccctgctgc
      601 gccgcccggg ggccctgcac ggcgagccct tgccggtgca cgtgatcgtc aaccagatgg
      661 aggcctcgcc gcatgtggtg gtcgagctgg tcgagatcga gcagcagacc cgccaggacc
      721 gcgaggagcg cgcgctcgac caggcgcagg tgaccaagga gctgatccgc aacctcgccc
      781 acgagatcaa gaacccgctc ggcggcatcc gcggcgcggc gcagctgctc gagatggagg
      841 ttcaggagcg cgcgctgcac gagtacaccc aggtcatcat cggcgaggcc gaccggctgc
      901 aggcgctggt cgaccggctg ctggcgccgc atcgcaagcc gcatgtggtc agcgatgtca
      961 acatccacga ggtctgcgaa cgcgtgcggg cgctgatcca ggccgagttc ccgcgcgggc
     1021 tggcgatcga gcgcgactac gacatctcga tccccgactt tcgcggcgac cgcgagcagc
     1081 tgatccaggc ggtgctcaac attgcccaca acgccgcgca ggcgctcgcc gagcgcatca
     1141 ccaccggcgg cgacgcgcgc atcacgttgc gcacgcgcat cgcgcgccag gtgacgctgg
     1201 gcaaacagcg ctaccgtctg gcactggact tgcatattga ggacaacggc ccgggcgtgc
     1261 ccgagtccat tcgggatcga atcttctacc cgctggtatc ggggcgtgac ggcggttccg
     1321 gactcgggct cactctcgcg cagaccttcg tgcagcaaca cctcggcacg atcgagtgcg
     1381 agagtgagcc gggccggacc actttcaaga tcgtgatccc gttgccctga gcgccaaaag
     1441 cctcagcggc acatggcgag cagacagcgg cgatcggccg acagggaacg cagggcaggc
     1501 atgaaaccca tctggatcgt tgacgacgac caatccatcc gcttcgtgtt ggagaaagca
     1561 ctggcgcgcg aagacttcgc ggtgcgcagc ttcacgaact cgcgcgacgt gctcaaagcg
     1621 ctcgacgacg acgagccgca ggtgctcgtc tccgacatcc gcatgcccgg cggctcgggc
     1681 atcgagctgc tcgccaaggt gcgcgagcgc atgccggcgc tgccggtgat catcatgacc
     1741 gcgtactcgg acctggacag cgccgtcagc gcgttccagg gcggcgcgtt cgagtacctg
     1801 cccaagccgt tcgacgtgcc caaggcggtc gagctgatcc gccgcgccgt cgacgagagc
     1861 ctgcgcgaag aggtgcgcga cgagaaggcc gcgcagatgc ccgagatgct cggccaggcg
     1921 ccggcgatgc aggacgtgtt ccgcgcgatc gggcggctct cgcagagcat cgtcacggtg
     1981 ctgatcaccg gcgagtcggg ctcgggcaag gagctcgtcg cgcgcgcgct ccacaagcac
     2041 agcccgcgcg cgcaggggcc gttcgtcgcg atcaacaccg cggcgatccc gaaggacctg
     2101 ctcgagagcg agctgttcgg ccacgagcgc ggcgctttca ccggcgccac cgcaacgaag
     2161 ccgggcctct tcgagatggc ccacggcggc acgctgctgc tggacgaggt cggcaa
//
LOCUS       DASRDG010000052         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_705548, whole genome shotgun sequence.
ACCESSION   DASRDG010000052 DASRDG010000000
VERSION     DASRDG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705548"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFA35_00830"
     CDS             <1..589
                     /locus_tag="VFA35_00830"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HZT54740.1"
                     /translation="VAVGLLVPDTVHLTTRVLIGWNAGVWLFLVIVGWVMLQADHEHL
                     RRTAIAQAEGAAAVLTLVTVAAAFSIVAIIVELAAAKQGTRHAVPHILFALATVAASW
                     LLLPTLFAMNYASLYYHRQHGSGLQFPDPDKSFAPDYADFLYFSFTIAVASQTSDVTI
                     SSQPMRRLALLQAMLSFVFNTTILALTINIAASLF"
     gene            complement(586..1212)
                     /gene="purN"
                     /locus_tag="VFA35_00835"
     CDS             complement(586..1212)
                     /gene="purN"
                     /locus_tag="VFA35_00835"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZT54741.1"
                     /translation="MKRIVILISGRGSNMEAIVRACATEGWAARVAAVIANRADAPGL
                     AFARRHGIATAVVDHAAFDSRTAFDAALALAIEAHAPDVVALAGFMRILTDEFVERFA
                     GRLVNVHPSLLPAFAGLHTHRRAIEAGCKLAGATVHFVTPTLDHGPIIAQAVVPVLPG
                     DTPEALAARVLEREHVLYPRALRWLVHGLLRTTDGIVSHIGGEPQLLR"
     gene            1247..2242
                     /gene="trpS"
                     /locus_tag="VFA35_00840"
     CDS             1247..2242
                     /gene="trpS"
                     /locus_tag="VFA35_00840"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZT54742.1"
                     /translation="MKQRVLSGMRPTGALHLGHYHGALKNWVRLQDEFDCFYFVADWH
                     ALTTHYADHADLERNVYDMVVDWLAAGIDPARATLFIQSRLPEHAELFTLLAMGTPLA
                     WLERVPSYKDQIRSLAGRDLATYGFLGYPLLQAADILIYKAAFVPVGEDQAPHVEVTR
                     EVARRFNHLHGQGETVLPEPATLLTETAKLPGLDGRKMSKSYGNAIAMRDEPAEVERK
                     VRRMQTDPQRVRRSDKGDPERCPVWQFHAVYSDADTRAWVRTGCTTAGIGCLECKQPV
                     IDAIIREQQPWRERAENHLSNPKQVQWIVEVGTEQARGVARATMRSVRAAIGLNY"
BASE COUNT          378 a          846 c          809 g          340 t
ORIGIN      
        1 cgtcgcggtc gggttgctcg tgccagacac cgtccacctg accacacgcg tgctgatcgg
       61 ctggaacgcc ggtgtctggt tgttcctcgt gatcgtcggc tgggtgatgc tgcaggccga
      121 tcacgagcac ctgcgccgca ccgcgatcgc gcaggccgaa ggcgctgcgg cggtgctgac
      181 gctggtgacc gtcgcggcgg cattcagcat cgtcgcgatc atcgtcgagc tcgcggccgc
      241 caagcagggc acgcgccacg cggtgccgca catcctgttc gcgctcgcca ccgtcgccgc
      301 ctcgtggctg ctgctgccca cgctgttcgc gatgaactac gcgagcctgt actaccaccg
      361 gcagcacggc agcgggctgc agtttcccga ccccgacaag agcttcgcgc ccgactacgc
      421 cgattttctg tacttctcgt tcacgatcgc ggtggcgtcg cagacctccg acgtgacgat
      481 ctcgtcgcag ccgatgcgcc ggctcgcgct gctgcaggcg atgctgtcgt tcgtgttcaa
      541 cacgacgatc ctcgcgttga cgatcaacat cgcggcgagc ttgttctagc gcagcagctg
      601 cggctcaccg ccgatgtgcg acacgatccc gtcggtggta cgcaacaagc catgcacgag
      661 ccagcgcagc gcgcgcgggt acagcacgtg ctcgcgttcg agcacgcgcg cagcgagcgc
      721 ctcgggcgtg tcgccgggca gcacgggcac caccgcctgc gcgatgatcg ggccgtgatc
      781 gagcgtcggc gtgacgaagt gaaccgtcgc gccggcgagc ttgcaaccgg cctcgatcgc
      841 acggcgatgc gtgtgcaggc cggcaaacgc cggcagcaac gacgggtgca cattgacgag
      901 gcgacccgcg aaacgctcga cgaactcgtc ggtcaggatg cgcatgaagc cggccagcgc
      961 gaccacgtcg ggcgcgtgcg cctcgatcgc gagcgccagc gcggcgtcga aggcagtgcg
     1021 cgagtcgaag gccgcgtggt cgacgaccgc ggtcgcgatg ccgtggcggc gggcgaaggc
     1081 cagccccggc gcgtcggccc ggttggcgat caccgctgcg acgcgcgctg cccagccctc
     1141 ggtcgcgcac gcgcgcacga tggcctccat gttggagccg cggcccgaga tcagaatgac
     1201 gattcgcttc acagggcgcg cagtgtagct gcctacaatt cttccgatga agcaacgcgt
     1261 tctctccggc atgcgcccga ccggcgcgct gcacctgggc cactaccatg gcgcgctgaa
     1321 gaactgggtg cgcctgcagg acgagttcga ctgcttctac ttcgttgccg actggcacgc
     1381 gctgaccacg cactacgccg accacgccga tctggagcgc aacgtctacg acatggtggt
     1441 cgactggctc gccgccggca ttgacccggc ccgcgccacg ctgttcatcc agagccgcct
     1501 gccggagcat gccgagctgt tcaccctgct cgcgatgggc acgccgctgg cgtggctgga
     1561 gcgcgtgccg agctacaagg atcagatcag gagcctggcc ggccgcgacc tcgcgactta
     1621 cggcttcctc ggctacccgc tgctgcaggc ggccgacatc ctgatctaca aggccgcgtt
     1681 cgtgccggtc ggcgaagacc aggcaccgca tgtcgaggtc acgcgcgagg tggcgcggcg
     1741 cttcaatcac ctgcacggcc agggcgagac ggtgctgccc gagccggcga cgctgctgac
     1801 cgagaccgcg aagctgcccg gcctggacgg ccgcaagatg agcaagagct acggcaacgc
     1861 catcgcgatg cgcgacgagc cggccgaggt cgagcgcaag gtcaggcgca tgcagaccga
     1921 cccgcagcgc gtgcggcgca gcgacaaggg cgaccccgag cgctgcccgg tctggcagtt
     1981 ccacgccgtg tattccgatg ccgacacgcg cgcctgggtg cgcaccggtt gcacgaccgc
     2041 cggcatcggc tgcctcgagt gcaagcagcc ggtgatcgac gcgatcatcc gcgagcagca
     2101 gccgtggcgc gagcgcgccg aaaaccacct gagcaacccg aagcaggtgc agtggatcgt
     2161 cgaggtcggc accgagcagg cgcgcggcgt cgcacgggcg acgatgcgca gcgtgcgcgc
     2221 cgcgatcggc ctgaactact gagacgcgag cgcggcgagc gcgtcggcca cgtcctgcgc
     2281 gctgacgcct tcgccgtgcg cgaccggcga atccggcacc gcggccacgc cctcggcctt
     2341 caaacgcgcc acgcactgcc cagcctcgcg gcc
//
LOCUS       DASRDG010000053         3382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_9131, whole genome shotgun sequence.
ACCESSION   DASRDG010000053 DASRDG010000000
VERSION     DASRDG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9131"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFA35_00845"
     CDS             complement(<1..529)
                     /locus_tag="VFA35_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54743.1"
                     /translation="MKALAAVLASALIVGCASAPPMPPSADLFHDELFAPASAPVRPA
                     DAMAVSPAMRAYIATRIVATSHFRDPRRQLIDVLYRKDELQLEYDSAETRTAAQAFEA
                     RSGNCLALVMMTAALAKEMGLTVRYQAVLGDDAWDRSGDLYVAVGHVNLTLGDRVPQA
                     GFGFMENDPMTIDFVP"
     gene            complement(610..2226)
                     /locus_tag="VFA35_00850"
     CDS             complement(610..2226)
                     /locus_tag="VFA35_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HZT54744.1"
                     /translation="MSDLTRSALSKEVGRRRTFAIISHPDAGKTTLTEKLLLFSGAIQ
                     IAGSVKARKATRHATSDWMEIEKQRGISVASSVMQMEYRDCVINLLDTPGHQDFSEDT
                     YRVLTAVDAALMVIDAANGVEPQTRRLLQVCRARNTPILTFVNKMDREVQEPMALMDE
                     IERELGMEVVPFTWPVGMGKAFHGVMDLREKRMRVFGGEEGGAGFLDGLDNPVYPERF
                     GMQYAQSKDEIELLTDAAPAFDKDAFLAGKQTPMFFGSAVNNFGVQEVLDALVDLAPA
                     PGPRSAIQRDVQPDEPKLTGVVFKIQANMDPAHRDRIAFVRVASGHFERGMRIKVTRS
                     GKEMRPNTVVSFLSQRRELLDEAFAGDIIGIPNHGVLQLGDTLTEGEALQYTGLPFFA
                     PEMFRSVEVADPLRTKQLKAGLQQLGEEGAIQVFRPVAGSVLLLGAVGQLQFEVVAHR
                     LEHEYGVKARIQPSRYQVARWVTCDDPKELQRFIDGNAHRVAYDAVDAPTVLVEYAPE
                     LRAIEANWPKIRFHALREHAGLVFQRQLEG"
     gene            complement(2311..2838)
                     /locus_tag="VFA35_00855"
     CDS             complement(2311..2838)
                     /locus_tag="VFA35_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3016 domain-containing protein"
                     /protein_id="HZT54745.1"
                     /translation="MKTIITPLRSVCCALALAAAGAALAAPGAPVSTDKAEVSLRNPA
                     GFTEMRRSFGARTDWLDELSSYIAKRAARAVPDGERLIVTITDVQRAGMIEPWRHGAT
                     DLRVVRETTPPRIDLSFQLVAANGAVLKEGSRQLHDVAFMTRSFSPRHRGDPLAYEKN
                     LIDDWLRKDVEAARR"
     gene            complement(2888..>3382)
                     /locus_tag="VFA35_00860"
     CDS             complement(2888..>3382)
                     /locus_tag="VFA35_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZT54746.1"
                     /translation="PTAHVTSMGGVTVQAQQPLEFANAADAVIFGSGVYTRAIAQNSA
                     LLDRLQLDPIRQLIGAQCSGALLLARMGLLADMPACTDLTTKPWLLAAGVRVEDAPFH
                     ARGPIATAGGCLASQYLAAWLMVRGAGFDAAARALHYAAPVGEKEAYVAKLLDVVRPF
                     VPAQ"
BASE COUNT          494 a         1185 c         1179 g          524 t
ORIGIN      
        1 gcggcacgaa gtcgatcgtc atcggatcgt tctccatgaa gccgaagccg gcctgcggca
       61 cacggtctcc cagcgtcagg ttgacatggc cgaccgcgac gtagaggtca cccgagcggt
      121 cccaggcgtc gtcgcccagc acggcctggt agcgaaccgt cagccccatc tccttggcca
      181 gcgcggcggt catcatcacc agcgccaggc agttgccgga gcgggcctcg aaggcctgcg
      241 ccgcggtgcg ggtctcggca gagtcgtact cgagctggag ctcgtccttg cggtacagca
      301 cgtcgatcag ctgccggcgc gggtcgcgaa agtgcgaggt cgcgacgatg cgggtcgcga
      361 tgtacgcgcg catcgccggg ctgaccgcca tcgcgtcggc aggccgtacc ggcgccgagg
      421 cgggcgcgaa caactcgtcg tggaacaggt cggcgctggg cggcatcggc ggcgcactcg
      481 cgcagccaac gatcagcgcc gaggccagca cagcagcgag ggctttcatg aacgcgcctc
      541 caaaaattca atcttcggcg agctccaggc gaagcggcag tggtactttc accgcacccg
      601 gcccggtggt cagccctcca gctgcctctg aaacaccagc ccggcgtgct cgcgcagcgc
      661 gtggaacctg atcttcggcc agttcgcctc gatcgcgcgc agctcgggcg cgtactcgac
      721 cagcaccgtg ggcgcatcga ccgcgtcgta ggccacgcga tgggcgttgc cgtcgatgaa
      781 gcgctgcagc tccttcggat catcacaggt cacccagcgc gccacctggt agcggctcgg
      841 ctggatccgc gccttgacgc cgtactcgtg ctcgagccgg tgcgcgacga cctcgaactg
      901 cagctgcccg accgcgccga gcagcagcac cgaaccggcg accgggcgga acacctggat
      961 cgcgccctcc tcgccgagct gctgcaggcc cgccttcagc tgcttggtgc gcagcgggtc
     1021 ggcgacctcg acgctgcgga acatctccgg cgcaaagaac ggcagcccgg tgtactgcag
     1081 cgcctcgcct tcggtcagcg tgtcgccgag ctgcagcacg ccgtggttcg ggatgccgat
     1141 gatgtcgccg gcgaaggctt cgtcgagcaa ctcgcggcgc tgcgagagga acgagaccac
     1201 ggtgttcggc cgcatctcct tgcccgagcg cgtgaccttg atccgcatgc cgcgctcgaa
     1261 gtggccgctc gcgacgcgca cgaacgcgat ccggtcgcgg tgcgccgggt ccatgttcgc
     1321 ctggatcttg aagacgacgc cggtgagctt gggctcgtcg ggctgcacgt cgcgctggat
     1381 cgcgctgcgc ggcccgggcg cgggcgccag gtcgaccagc gcgtcgagca cctcctgcac
     1441 accgaagttg ttgacggccg agccgaagaa catcggcgtc tgcttgcccg ccaggaacgc
     1501 gtccttgtcg aacgccggcg cggcatcggt cagcagctcg atctcgtcct tcgactgcgc
     1561 gtactgcatg ccgaagcgct cggggtagac ggggttgtcc aggccgtcga gaaacccggc
     1621 gccgccttct tcgccgccga acacccgcat gcgcttctcg cgcaggtcca tcacgccgtg
     1681 aaacgccttg cccatgccga ccggccaggt gaaaggcacg acctccatgc cgagctcgcg
     1741 ctcgatctcg tccatcagcg ccatcggctc ctggacctcg cggtccatct tgttgacgaa
     1801 ggtcaggatc ggcgtgttgc gcgcgcggca gacctgcagc agccgccggg tctgcggctc
     1861 gacgccgttg gccgcgtcga tcaccatcag cgccgcgtcg accgcggtca gcacgcggta
     1921 ggtgtcttcc gagaagtcct ggtggcccgg cgtgtcgagc aggttgatga cgcagtcgcg
     1981 gtactccatc tgcatcacgc tcgatgcgac cgagataccg cgctgcttct cgatctccat
     2041 ccagtcgctg gtggcgtggc gggtcgcctt gcgcgccttc accgagccgg cgatctggat
     2101 cgcgcccgag aacagcagca gcttctcggt cagcgtggtc ttgccggcgt cggggtggga
     2161 aatgatcgcg aaggtgcggc ggcggccgac ttctttggac agggcggaac gggtcaggtc
     2221 ggacatgcag gtgcggccga ttcgcgggcc gcctcgagga gacaacccgc gattatcggt
     2281 ggcgcaccgc gccgctgccg ttgcgcgcgc tcagcggcgc gccgcctcca cgtccttgcg
     2341 cagccagtcg tcgatcaggt tcttctcgta cgccagcggg tcgccgcggt ggcgcggcga
     2401 gaagctgcgc gtcatgaacg ccacgtcgtg caactggcgc gagccttcct tcagcaccgc
     2461 gccgttcgcg gcgacaagct ggaagctcag gtcgatgcgc ggcggcgtcg tctcgcgcac
     2521 cacgcgcagg tcggtcgcgc cgtggcgcca gggttcgatc atgccggcgc gctgcacgtc
     2581 ggtgatcgtc acgatcaggc gctcgccgtc gggcacggcg cgcgccgcgc gcttcgcgat
     2641 gtagctgctc agctcgtcga gccagtcggt gcgcgcgccg aagctgcggc gcatttcggt
     2701 gaagccggcc gggttgcgga ggctgacctc ggccttgtcg gtcgagaccg gcgcgccggg
     2761 cgccgccaag gccgcgccgg cggccgccag cgccagcgcg cagcagacgc tgcgcagcgg
     2821 cgtgatgatc gtcttcatgg cggactctcc tttcgcatcg ctccacgcga ccgcggctga
     2881 gagactgcta ctgtgccgga acgaacggtc gcaccacgtc cagcagcttc gcaacatacg
     2941 cctctttctc gcccaccggc gcggcgtagt gcaaagcccg cgcggcggcg tcgaagcccg
     3001 cgccgcgcac catcagccag gcggcgaggt actgcgacgc caggcagccg ccggcggtgg
     3061 cgatggggcc acgcgcgtgg aacggcgcgt cttcgacgcg cacgccggct gcgagcagcc
     3121 agggcttggt cgtcaggtcg gtgcaggccg gcatgtcggc gagcaggccc atgcgcgcga
     3181 gcaggagcgc gcccgagcac tgcgcgccga tcagctggcg gatcgggtcg agttgcaggc
     3241 ggtcgagcag ggcgctgttt tgcgcgatcg cgcgggtgta gacgccgctg ccgaagatca
     3301 ccgcgtcggc cgcgttcgcg aactccagcg gctgctgcgc ctgcaccgtc acgccgccca
     3361 tcgaggtgac gtgcgccgtc gg
//
LOCUS       DASRDG010000054         3666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1513343, whole genome shotgun sequence.
ACCESSION   DASRDG010000054 DASRDG010000000
VERSION     DASRDG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513343"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..823
                     /locus_tag="VFA35_00865"
     CDS             29..823
                     /locus_tag="VFA35_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZT54747.1"
                     /translation="MTARIRVGVGGWTFEPWRDNFFPAGWPHARELEYASRKLSAIEV
                     NGTYYSSQKPATFARWRDETPDDFVFSLKASRFATNRRVLAEAGESIERFVNQGIAEL
                     REKLGPIVWQFAPTKRFEPDDFEAFLRLLPAQVAGLPLRHVLDVRHDSFKTPAYLALA
                     RRHHMATVFTDSDDYPSFADVTGGFVYARLMRTDATHAEGCPPEYFGPLAACAKAWRD
                     GAEPAGLPRVEPAPARAAPRDVFLYFISGAKEKAPAAAMALIKALG"
     gene            823..2481
                     /locus_tag="VFA35_00870"
     CDS             823..2481
                     /locus_tag="VFA35_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZT54748.1"
                     /translation="MPRPAATLVVVRDGAAGIELLLSRRAERGDPGSGAWVFPGGVVD
                     PGDRLAHACCAGLDDAAASARLGLPAHGLDHYVAAVRECFEESGLLFATDAAGELVRL
                     DDTTGARLAAWRGPLHRHERTLAEMCRAFGLTLALDRLVYLSHWLTPLGLPKRFDTRF
                     FVAAAPASQTAAFDGTEMVEQLWLRPAEALARAVELKLMTPTRKTLELVGKHATVDAL
                     LAWARAPRKVALIMPRIAHGRQGPRPVLPDEPAFAELGRVDPAGEGTGCYDIQVATPV
                     RLSPRLIRVTANNGSLMTGPGTNTYLVGGGARNEWAVIDPGPIDNAHVDAIVAAAPGP
                     IRWIFVTHTHNDHSPARVPLKRRTGATVYGRLPDHPEWQDASFAPDVHLKGGERFELP
                     GGTTLHAIHTPGHASNHLCYRLEEEKLLFTGDHVMQLSTVVINPPDGDMAAYIASLRA
                     LADLELDWLAPGHGFLMAQPKQAIEAIVAHRLRREVKVVDALRELGPADDHALLARVY
                     DDVKPQLLAMAMRSLRAHLFKLRADGVAREVDGRWVLAASRSTA"
     gene            complement(2470..3084)
                     /locus_tag="VFA35_00875"
     CDS             complement(2470..3084)
                     /locus_tag="VFA35_00875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZT54749.1"
                     /translation="MNLLLIRHGETALNVARVLQPAATKLSERGVAQADALASRLARM
                     ELRAIVSSDLPRALRTAQAIAATTGLAIETTPLLQERNFGDWRGRAYDELALDPLTMA
                     AAPPNGESMAAFEQRVAAAFAHVLQRQAALGGVLAVVTHGLVIRALLAAHVRLADGAA
                     PPAHLGNTSLSIIGAQPPHLASLLNCTQHLDGAARDDAHALSGG"
     gene            complement(3143..>3666)
                     /locus_tag="VFA35_00880"
     CDS             complement(3143..>3666)
                     /locus_tag="VFA35_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZT54750.1"
                     /translation="IQLIINREWGLAKNENPNQGAFIIDELTELVEEMVLQEFERLAE
                     RGGVLGAMETGYQRGKIQEDSMHYEMLKHTGEYPIIGVNTFRNPHGDPVADTLELARS
                     TDAEKRSQLKRLAEFHARHAAESPAMLRRLKDAVIANANVFEVLMDAVRCCSLGQITE
                     ALFEVGGQYRRSM"
BASE COUNT          507 a         1345 c         1274 g          540 t
ORIGIN      
        1 ggccgtcgac aatcaaggga gagcatcaat gaccgcaagg atccgcgtcg gcgtcggggg
       61 ctggaccttc gagccctggc gcgacaactt ctttccggcc ggctggccgc atgcgcgcga
      121 gctcgagtac gcgagccgca agctcagcgc gatcgaggtc aacggcacct actacagctc
      181 gcagaagccg gccacgttcg caaggtggcg cgacgaaacg cccgacgact tcgtgttctc
      241 gctgaaggcc tcgcgcttcg cgacgaaccg gcgcgtgctc gccgaggcgg gcgaatcgat
      301 cgagcgtttc gtgaaccagg gcatcgccga gttgcgcgag aagctcgggc cgatcgtctg
      361 gcagttcgcg ccgaccaagc gcttcgagcc ggacgacttc gaggccttcc tgaggctgct
      421 gcccgcgcag gtcgccggcc tgccgctgcg ccatgtgctc gacgtgcgcc acgacagctt
      481 caagacgccc gcgtacctcg cgttggcgcg ccgccaccac atggccacgg tcttcaccga
      541 ctccgacgac tacccctcgt tcgccgatgt caccggcggc ttcgtctacg cgcgcctgat
      601 gcgcaccgac gcgactcacg ccgagggctg cccgcccgag tacttcgggc cgctcgccgc
      661 ctgcgcgaaa gcctggcgcg acggcgccga gcccgccggc ctgccgcgcg tcgagcccgc
      721 gccggcgcgg gccgcgccgc gcgacgtgtt cctgtacttc atcagcggcg cgaaagagaa
      781 agcgccggcc gccgcgatgg cgctgatcaa ggcgctcggc tgatgccgcg gcccgccgcc
      841 accctcgtcg tcgtgcgcga cggcgccgcg ggcatcgagc tgctgctgtc gcgccgcgcc
      901 gaacgcggcg accccggcag cggtgcgtgg gtgttccccg gcggcgtcgt cgacccgggc
      961 gaccggctcg cgcacgcttg ctgcgccggg ctggacgacg cggccgcgag cgcccggctg
     1021 ggcctgccgg cgcacggcct cgatcactac gtcgccgcag tgcgcgagtg cttcgaggag
     1081 tcgggcctgc tgttcgcgac ggacgccgcc ggcgaactcg tccgcctgga cgacacgacc
     1141 ggcgcgcggc tcgccgcgtg gcgcggcccg ctgcaccgcc acgagcgcac gctcgccgag
     1201 atgtgccgcg cgttcgggct gacgctcgcg ctcgaccggc tcgtctacct gagccactgg
     1261 ctcacgccgc tggggttgcc gaagcgtttc gacacgcgct tcttcgtcgc cgcggcgccg
     1321 gcatcgcaaa ccgccgcgtt cgacggcacc gaaatggtcg agcagctctg gctgcgcccg
     1381 gccgaggcgc tggcgcgcgc cgtcgaactg aagctgatga caccgacgcg caagacgctg
     1441 gagctggtcg gcaagcacgc caccgtcgac gcgctgctcg cgtgggcacg cgcgccgcgc
     1501 aaggtcgccc tgatcatgcc gcggatcgcg cacggccggc agggcccacg gccggtgctg
     1561 cccgatgagc cggcgttcgc cgagctcggc cgcgtcgacc cggccgggga gggcaccggc
     1621 tgctacgaca ttcaggtcgc gacgccggtg aggctctcgc cgcgcctgat ccgcgtgacc
     1681 gcgaacaacg gcagcctgat gaccgggccc ggcaccaaca cctacctggt cggcggcggc
     1741 gcgcgcaacg agtgggcggt gatcgacccc ggcccgatcg acaacgcgca tgtcgacgcg
     1801 atcgtcgcgg ccgcgcccgg cccgatccgc tggatcttcg tgacccacac ccacaacgac
     1861 cactcgccgg cccgcgtgcc gctgaagcga cgcaccggcg cgacggtgta cggccgcctg
     1921 cccgatcacc cggagtggca ggacgcgagc ttcgcgcccg atgtgcattt gaagggcggc
     1981 gagcgcttcg agttgcccgg tggcacgacg ctgcacgcga tccacacgcc cggccacgcg
     2041 tcgaaccacc tgtgctaccg gctcgaggaa gagaagctgc tgttcaccgg cgaccacgtg
     2101 atgcagctct cgaccgtcgt gatcaacccg cccgatggcg acatggcggc ctacatcgcc
     2161 tcgctgcgcg cgctcgccga cctcgagctc gactggctcg cgccgggcca tggtttcctg
     2221 atggcgcagc cgaagcaggc gatcgaggcc atcgtcgcgc accgcctgcg gcgcgaggtc
     2281 aaagtggtcg acgcgctgcg cgagctcggc ccggccgacg accatgcctt gctcgcgcgc
     2341 gtctacgacg acgtgaagcc gcagctgctc gcgatggcga tgcggtcgct gcgcgcgcac
     2401 ctgttcaagc tgcgcgccga cggcgtggcg cgcgaggtcg acgggcgctg ggtgttggcg
     2461 gcgtcgcgct caaccgcctg acagcgcgtg cgcatcgtcg cgcgcggcgc cgtcgagatg
     2521 ttgcgtgcag ttcagcaagc tcgccaagtg cggcggttgc gcgccaatga tgctcaggct
     2581 ggtgttgccc aggtgcgcgg gcggcgcggc gccgtcggcc agccgcacgt gcgccgccag
     2641 cagcgcgcgg atcacgaggc cgtgcgtgac gaccgcgagc accccgccga gcgccgcctg
     2701 gcgttgcagc acatgcgcaa acgcggccgc gacgcgctgc tcgaacgccg ccatcgattc
     2761 gccgttcggc ggcgccgcgg ccatcgtcag cgggtcgagc gcgagttcgt cgtaggcgcg
     2821 gccgcgccag tcgccgaagt tgcgctcctg cagtagcggc gtggtctcga tcgccaggcc
     2881 cgtggtcgcc gcaatcgcct gcgcggtgcg cagcgcacgc ggcagatcgc tgctgacgat
     2941 cgcgcgcaac tccatgcgcg cgagccgcga cgcgagcgca tcggcttgcg cgacaccgcg
     3001 ttcgctcagc tttgttgcgg caggctgcag cacgcgtgcg acgttcagtg cggtctcgcc
     3061 gtggcggatc agcagcaggt tcatggggcc gagtatgtcg gatgcgggcg acccgttcgc
     3121 ggcggatcac ccgacccgcc cgctacatgc tgcgccggta ctgcccgccc acctcgaaca
     3181 gcgcctcggt gatctgcccc agcgagcagc aacgcaccgc gtccatcagc acctcgaaaa
     3241 cgttcgcgtt cgcgatcacc gcgtccttca aacgccgcag catcgccggt gattcggcgg
     3301 cgtgccgggc gtggaactcg gccaggcgct tcagctggct gcgcttctcg gcgtcggtcg
     3361 agcgcgcgag ctcgagcgtg tcggccaccg ggtcgccatg cgggttgcgg aaggtgttga
     3421 cgccaatgat ggggtactcg ccggtgtgct tgagcatctc gtagtgcatg ctatcttcct
     3481 ggatcttgcc gcgctggtag ccggtctcca tcgcgccgag cacgccgccg cgttcggcga
     3541 ggcgctcgaa ctcctgcagc accatctcct cgaccagctc ggtcagctcg tcgatgatga
     3601 acgcgccctg gttcgggttc tcgttcttcg ccaggcccca ttcgcggttg atgatcaatt
     3661 ggatcg
//
LOCUS       DASRDG010000055         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_124033, whole genome shotgun sequence.
ACCESSION   DASRDG010000055 DASRDG010000000
VERSION     DASRDG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124033"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFA35_00885"
     CDS             <1..1002
                     /locus_tag="VFA35_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZT54751.1"
                     /translation="RRHERHRELQVRLIDGPTGVHRLRNWRLFAICVLVWGTTWFAIT
                     FQIGQAAPEVGVALRFGLAGALVLAMCAWRALPLRFDARAHALLALQGAFLYGVSYVC
                     VYHAEQHLVSGLVAVGYSASPLVGGLGARALFGTRTSVRFVVGGLLGLAGVALIFWPE
                     FGKAAGRSGTALGALFTLASVLLSTVGSLTASRNRANALPFWPALGYGMLYGAAVSAA
                     IALAQGRSFALPASASWWAALLYLSLAGSVLTFACFLALQQRIGVGPTGAIGVMTPLL
                     ALVVSMAFERFRPDALTFAGAALAVTGNVLMLTRRPAPAADVPATPPRARDTPRGGR"
     gene            complement(923..1582)
                     /locus_tag="VFA35_00890"
     CDS             complement(923..1582)
                     /locus_tag="VFA35_00890"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZT54752.1"
                     /translation="MTSQTRVVGADTGLFTADDGAARCRWCQASAAYRHYHDHEWGFP
                     VTDDRRLFEKLCLEGFQAGLSWLTILNKRAAFRAGFADFDAEQVARFTQADIARLLAD
                     AGIVRHRGKIESTINNARRVLELKREFGSLAAYAWSHEPPARLRPKKMTLVALRANTS
                     SPEAVAMSKDLKRRGWRFVGPTTVYAFMQAMGLVNDHLEGCHVRAAALQARQRLAQGG
                     G"
     gene            1679..>2102
                     /locus_tag="VFA35_00895"
     CDS             1679..>2102
                     /locus_tag="VFA35_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZT54753.1"
                     /translation="MSTPALFSTRAVAALLAVAALASARAETPLERGRYLARSIVACG
                     NCHTPQGPTGPLAGMDLAGGLQIDVPPMFTAFSANITPHKTSGIGAWSDAQIIAAIRE
                     GKRPDGSLIGPPMPIELYRGLSDTDVRALVAYLRSVPAV"
BASE COUNT          245 a          767 c          746 g          344 t
ORIGIN      
        1 cgtcggcatg agcggcaccg cgagcttcaa gttcgactga tcgacgggcc gaccggcgtg
       61 catcgcctgc gcaactggcg gctgtttgcc atctgcgtgc tcgtctgggg cacgacctgg
      121 ttcgcgatca cgttccagat cgggcaggcc gcgcccgagg tcggcgtcgc gctgcgcttc
      181 gggctggccg gcgcgctggt gctggcgatg tgcgcgtggc gcgcgctgcc gctgcgcttc
      241 gacgcacgcg cgcatgcgct gctcgcgctg cagggcgcgt tcctgtacgg cgtatcgtat
      301 gtctgcgtct accacgccga gcagcacctg gtgtcggggc tggtcgcggt cggctactcg
      361 gcctccccgc tggtcggcgg gctcggcgcg cgggcgctgt tcggcacgcg cacctcggtg
      421 cgcttcgtcg tcggcgggct gctcgggctg gcgggcgtcg cgctgatctt ctggcccgag
      481 ttcggcaagg ccgcggggcg cagcggcacg gcgctcggcg cgctgttcac gcttgcgtcg
      541 gtgctgctgt cgacggtcgg cagcctcacg gccagccgca accgcgccaa cgcgctgccg
      601 ttctggccgg cactgggtta cggcatgctc tacggcgccg cggtcagcgc cgcgatcgcg
      661 ctggcgcagg gccgcagctt cgcgctgccg gcgagcgcct cgtggtgggc cgcgctgctg
      721 tatttgtcgc tggccggctc ggtgctgacc ttcgcgtgct ttctggcgct gcagcagcgc
      781 atcggcgtcg gcccgaccgg cgcgatcggc gtgatgacgc cgctgctcgc gctcgtcgtc
      841 tcgatggcct tcgagcgctt tcggcccgac gcgctcacct tcgccggcgc cgcgctcgcg
      901 gtgaccggca acgtgctgat gctcacccgc cgccctgcgc cagccgctga cgtgcctgca
      961 acgccgccgc gcgcacgtga cacccctcga ggtggtcgtt gaccagcccc atcgcctgca
     1021 tgaacgcata gaccgtcgtc gggccgacga agcgccagcc acgccgcttc aggtccttcg
     1081 acatcgccac cgcctcgggc gagctcgtgt tcgcacgcaa ggccacgagc gtcatcttct
     1141 tcggccgcaa ccgcgccggc ggctcgtgcg accacgcgta cgcggcgagc gagccgaact
     1201 cgcgcttcag ttcgagcacg cgccgcgcgt tgttgatcgt cgactcgatc ttgccgcggt
     1261 gccgcacgat gccggcgtcg gcgagcagcc gcgcaatgtc ggcctgcgtg aagcgcgcca
     1321 cctgctcggc gtcgaagtcc gcgaagccgg cgcggaacgc cgcgcgcttg ttcaggatcg
     1381 tgagccagct caggccggcc tgaaaacctt cgaggcacag cttctcgaac aggcgccggt
     1441 cgtcggtgac cgggaagccc cattcgtggt cgtggtagtg ccggtacgcc gcgctcgcct
     1501 ggcaccagcg gcagcgcgcg gcgccatcgt cggcggtgaa caggcccgtg tcggcgccga
     1561 cgactcttgt ctggctcgtc atgggcggca ttgtggcgcc ggccggcgcg catcgcgatc
     1621 cgcttctcaa ccggccgttt gcggcgcaca ctgcgagcca catcgggagg cgagagacat
     1681 gtccacacct gcgctttttt cgacacgcgc ggtcgccgcg ctgctcgccg tggccgcgct
     1741 ggccagcgcg cgcgccgaga cgccgctcga acgcggccgc tatctggcgc gctcgatcgt
     1801 cgcctgcggc aactgccaca cgccgcaggg tccgaccggc ccgctggccg ggatggatct
     1861 ggccggcggc ctgcagatcg acgtgccgcc gatgttcacc gcgttcagcg ccaacatcac
     1921 gccgcacaag accagcggca tcggcgcctg gagcgatgcg cagatcatcg cggcgatccg
     1981 cgaaggcaag cgtcccgacg gctcgctgat cggcccgccg atgccgatcg agctgtaccg
     2041 cggcctgtcg gacaccgacg tgcgggcgct ggtcgcgtac ctgcgcagcg tgccggcggt
     2101 gg
//
LOCUS       DASRDG010000056         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_469423, whole genome shotgun sequence.
ACCESSION   DASRDG010000056 DASRDG010000000
VERSION     DASRDG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469423"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VFA35_00900"
     CDS             complement(<1..919)
                     /locus_tag="VFA35_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZT54754.1"
                     /translation="MSLPLDLLLAAVLGALHALTYADTRAWPLQLALVAALAWRTSNA
                     TPWRAAGLGLAFGSAWLGAGTWWLFISLHTYGGLPAWLAVLAIALLSAFLSLYLAAAL
                     AAFARWRSGSAPRDALLFGAVWLLAELARGVLFTGFPWLASGYAQVDGPLAALAPWLG
                     VYGIGFVGAVLAAWVVCGWGRSWRERAWPLGALALVLGGAAMLGPGRFSQPNGTLTVT
                     LLQGNVAQDEKFALASMPQALAWTRRNLLAARGELVVAPETVIPLLPDQLDAGYWNEI
                     ADHFQRGPQAALLGLPLGDPQLGYTNSAAG"
     gene            complement(946..1842)
                     /locus_tag="VFA35_00905"
     CDS             complement(946..1842)
                     /locus_tag="VFA35_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HZT54755.1"
                     /translation="MSEPHPGRPRRGGDHHTDPAAPRRTLFQKLIEFVAPGPDSRDQL
                     IESLADAENKSLIAPESRMMLEGVIRMADMIAGDVMVPAGRMDQLDIASEYDELLHLV
                     IDTGHSRFPVFEGGRENVIGILMAKDLLKLQRSPELNLRTLLRPAVFVPESKGLNELL
                     RDFRSNRNHLAIVIDEFGSIAGLITIEDVLEEIVGEIEDEFDDKDGESGIYTLADGSQ
                     RVAGDVDIVAVNEAFGTLLPNDEFDSIGGLVAHELGRVPRRGESVGIGELVFTVMLTR
                     GGAVRWFKVTRAPEAPPGPEGS"
     gene            2008..>2450
                     /locus_tag="VFA35_00910"
     CDS             2008..>2450
                     /locus_tag="VFA35_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZT54756.1"
                     /translation="MALIPATILTGFLGSGKTTLLKRVLTEAHGQKIAVIENEFGDEN
                     IDNEILVQDSKEQIIQLNNGCVCCSIREDLRSTLAELAEKKRKGELDFDRVVIETTGL
                     ADPGPVAQTFFMDDEIAESYLLDSILTLVDAKHGDQQLDDRQEARR"
BASE COUNT          422 a          868 c          843 g          317 t
ORIGIN      
        1 tgcctgcggc ggagttggtg tagccgagct gcgggtcgcc gagcggcaag ccgagcagcg
       61 cggcctgcgg cccgcgctgg aaatgatccg cgatctcgtt ccagtagccg gcgtcgagct
      121 ggtcgggcag cagcgggatc accgtctcgg gcgcgaccac cagctcaccg cgcgcggcca
      181 gcaggttgcg ccgcgtccag gccagcgcct gcggcatcga agccagcgcg aacttctcgt
      241 cctgcgcgac attgccctgc agcagcgtga ccgtcagcgt gccgttcggc tgcgagaagc
      301 ggcccgggcc gagcatggcc gcgccgccga gcaccagcgc caatgcgccg agcggccacg
      361 cgcgctcgcg ccagctgcgg ccccagccgc acaccaccca cgccgccagc accgcgccga
      421 cgaagccgat gccgtacacg ccgagccacg gcgcgagcgc ggcgagcggg ccgtcgacct
      481 gcgcatagcc gctcgcaagc cacggaaaac ccgtgaagag cacgccgcgc gccagttcgg
      541 cgagcagcca gaccgcgccg aacaacagcg cgtcgcgcgg cgccgagccg ctgcgccagc
      601 gcgcgaacgc ggccagcgcg gccgccagat agagcgacag gaaggcgctg agcagcgcaa
      661 tcgccagcac cgcgagccac gccggcagcc cgccgtaggt gtgcaggctg atgaacagcc
      721 accaggtgcc ggcgccgagc caggcgctgc cgaacgcgag gccgagcccc gcggcacgcc
      781 acggcgtggc gttcgaggtg cgccaggcga gcgccgcgac gagcgccagc tgcagtggcc
      841 aggcccgcgt gtcggcgtag gtcagcgcgt gaagcgcgcc gagcaccgcg gcaagcagca
      901 gatcgagcgg cagcgacaag ggcgctgcgg gcaaccgggc gcgggtcaac tgccttccgg
      961 cccgggcggc gcctcgggcg cgcgcgtgac cttgaaccag cgcaccgcgc cgccgcgggt
     1021 cagcatcacc gtgaacacca gctcgccgat tccgaccgat tcgccgcgcc gcggcacgcg
     1081 gccgagctcg tgcgcgacca gcccgccgat gctgtcgaac tcgtcgttcg gcagcagcgt
     1141 gccgaaggcc tcgttgacgg ccacgatgtc gacatcgccg gccacgcgct ggctgccgtc
     1201 ggccagggtg tagatgccgg actcgccgtc cttgtcgtcg aactcgtcct cgatctcgcc
     1261 gacgatttct tcgagcacgt cctcgatcgt gatcagcccg gcgatgctgc cgaactcgtc
     1321 gatcacgatc gcgaggtggt tgcggttgga gcggaagtcg cgcagcaact cgttcaggcc
     1381 cttcgactcg ggcacgaaca cggccgggcg cagcagcgtg cgcaggttga gttcgggcga
     1441 gcgctgcagc ttgagcaagt ccttcgccat caggatgccg atgacgttct cgcggccgcc
     1501 ctcgaacacc ggaaagcgcg agtggccggt gtcgatcacg aggtgcagca gctcgtcgta
     1561 ctcggaggcg atgtcgagct ggtccatgcg gccggccggc accatcacgt cgccggcgat
     1621 catgtcggcc atgcggatca cgccttcgag catcatgcgc gactcgggcg cgatcagcga
     1681 cttgttctcg gcgtcggcga gcgactcgat caactggtcg cgcgagtcgg gcccgggcgc
     1741 gacgaactcg atcagcttct ggaacagtgt gcggcgcggc gccgccgggt cggtgtggtg
     1801 gtcgccccct cgacgggggc ggccaggatg tggctcggac atcgcgcggg aggcgaagag
     1861 ttcagatcgg cagagcttaa ctgaacaatg cccctcggca ggacacgctc cggcagcggc
     1921 ttgacacatc tgctaccttg tcgggtcgtc gggagccctc atctgagcgg gccaccggcc
     1981 aagcgcacta tttcaaggca gatccccatg gcactgatcc ccgcaaccat cctcaccggc
     2041 tttctcggct cgggcaagac cacgctgctc aagcgcgtgc tgaccgaggc ccacggccag
     2101 aagatcgccg tgatcgagaa cgagttcggc gacgagaaca tcgacaacga gatcctggtg
     2161 caggacagca aggagcagat catccagctc aacaacggct gcgtctgctg ctcgatccgc
     2221 gaagacctgc gcagcacgct cgccgaactg gccgagaaaa agcgcaaggg cgagctcgac
     2281 ttcgaccgcg tggtgatcga gaccaccggc ctggccgacc ccggcccggt ggcccagacc
     2341 ttcttcatgg acgacgagat cgccgagagc tacctgctcg attcgatcct gacgctggtg
     2401 gacgccaagc acggcgacca acagctcgac gaccgccagg aggcgcgccg
//
LOCUS       DASRDG010000057         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_469629, whole genome shotgun sequence.
ACCESSION   DASRDG010000057 DASRDG010000000
VERSION     DASRDG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469629"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFA35_00915"
     CDS             complement(<1..366)
                     /locus_tag="VFA35_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZT54757.1"
                     /translation="MRLVFAGTPDFAVPHLAACRRDGVEIAAVYTQPDRPAGRGRKLT
                     PSPVKQAALDFGLPVEQPENFKDAATRARLAAYAPDLIVVVAYGLILSRKVLAIPRLG
                     CWNVHASLLPRWRGAAPIQR"
     gene            complement(385..894)
                     /gene="def"
                     /locus_tag="VFA35_00920"
     CDS             complement(385..894)
                     /gene="def"
                     /locus_tag="VFA35_00920"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZT54758.1"
                     /translation="MSLLPILEYPDPRLRTKAQPVTVFDAALQTLIDGMFATMYEAPG
                     IGLAASQVNVHRQLLVLDVSEEKNAPMVLINPKIVASEGSQTYQEGCLSVPGIFADVD
                     RADRITVESLDRHGQAQNFEADGLLAVCIQHEMDHLIGKLFVDYLSPLKRDLVRKKLE
                     KQRRNAAAG"
     gene            1033..2214
                     /locus_tag="VFA35_00925"
     CDS             1033..2214
                     /locus_tag="VFA35_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HZT54759.1"
                     /translation="MLKKLPAIFAGLALTLVAYAATVELKPGHPDTYVVRRGDTLWGI
                     ANKFLKKPWQWPEVWQANGQIKNPHLIFPGDVINLAYLNGPHLNNAGGPHVRVEQLED
                     AIKPIALERIDKFLKRTRIIGEDDFKAAAHVVGAEEAHLRATPGQLVYVRGLDATPGQ
                     QLAIVRPLGRYYELVPKDGAPQRFYRDSTDEASRSHENRGDLIWHHGPQQFSLAGNVK
                     FLGYEGLQTGTVRVTKAGNPASALLLSSDVEVAPGDLVLPLDDKPYDAQYVPHPPTKV
                     PDGMRVIAFTDTLDASGGMVTGRDQVLALSHGADDGIENGQVFSIFHPGEKVNDNFDY
                     SPESAKKFFHPKDAKVQLPEEYVGHVMIFRTFEHVSYGLVVDGIKPVHLGDPLYDPDH
                     I"
     gene            complement(2467..>3120)
                     /locus_tag="VFA35_00930"
     CDS             complement(2467..>3120)
                     /locus_tag="VFA35_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZT54760.1"
                     /translation="LLSCDNDDFPLLLRDIGSAPAALFVSGDPTALWTPQIAIVGARS
                     ASAAGLANARAFARAFALAGNTVTSGLAEGIDGAAHTATLAAGGKTIAVLGTGIDLVY
                     PRQHAELAARIAANGALVSEFPPGVPGHPTHFPRRNRIISGLSLGTLVIVLPARAKAR
                     ANARALARPAALALRAPTIAICGVHSRVGSPLTNSAAGALPMSRSSSGKSSLSQLSR"
BASE COUNT          527 a         1051 c         1065 g          477 t
ORIGIN      
        1 gcgctggatc ggcgcggcgc cgcgccagcg tggcagcagc gaggcatgca cgttccagca
       61 gccgagacgc gggatcgcga gcaccttgcg cgacaagatc agaccgtagg caacgacgac
      121 gatcaggtcg ggcgcgtagg cggcgaggcg agcgcgtgtc gccgcatcct tgaagttctc
      181 gggctgttcg accggcaggc cgaagtcgag cgcggcctgc ttgaccgggc tcggcgtcag
      241 cttgcgcccg cgcccggccg ggcggtcggg ctgggtgtag acggcggcga tctcgacgcc
      301 gtcacggcgg cacgcggcga gatgcggcac ggcgaaatcg ggcgttcccg cgaacaccag
      361 ccgcaagggc gcgggcatgg acgctcagcc tgcggccgcg ttgcggcgct gcttttcgag
      421 cttcttgcgg acgaggtcgc gcttgagcgg cgagagataa tcgacgaaca gcttgccaat
      481 cagatgatcc atctcgtgct ggatgcacac ggcgagcagg ccgtcggcct cgaagttctg
      541 cgcctggcca tggcggtcga gcgattcgac cgtgatgcgg tcggcgcggt cgacgtcggc
      601 gaagatgccg gggaccgaca ggcagccttc ctggtaggtc tggctgcctt cactggcgac
      661 gatcttcggg ttgatcaaca ccatcggcgc gttcttttcc tcggacacgt cgagcacgag
      721 cagctgcctg tgcacgttga cctggctggc ggcaaggccg atgccgggcg cctcgtacat
      781 ggtcgcgaac atgccgtcga tcagggtctg cagcgccgca tcgaacacgg tcacgggctg
      841 cgccttggtg cgcagacgcg gatcgggata ttcgaggatc ggaagcagac tcatgacctt
      901 tagacgcgaa tttcaggaag gaaagtatat tgcatcggca cttgcgtgac gaacttgcgg
      961 acgattccgc gttttgccgg gcgatttttg ctctacactg tgtcggcctc gggggacact
     1021 gggtatccaa ccatgttgaa gaagctgcct gcgatttttg ccggactcgc actgacactc
     1081 gttgcctacg cggcgacggt cgaactcaag ccgggtcatc cggacaccta tgtcgtgcgc
     1141 cgcggcgaca cgctctgggg gatcgcgaac aagttcctca agaagccctg gcaatggcca
     1201 gaggtatggc aggccaacgg ccagatcaag aacccgcacc tgattttccc gggtgacgtg
     1261 atcaacctcg cctacctcaa cggcccgcac ctgaacaatg ccggcggccc gcacgtgcgc
     1321 gtcgagcaac tcgaggatgc gatcaagccg atcgcgctgg agcgcatcga caagttcctc
     1381 aaacgcacgc gcatcatcgg cgaggacgac ttcaaggccg ccgcgcacgt agtcggcgcc
     1441 gaagaggcac acctgcgcgc gacgccgggc cagctcgtct acgtgcgcgg cctcgacgcc
     1501 acgccgggcc agcagctcgc gatcgtgcgc ccgctgggcc gctactacga actcgtgccg
     1561 aaggacggcg cgccgcaacg cttctaccgc gactccaccg acgaggcgag ccgcagccac
     1621 gagaatcgcg gcgacctgat ctggcatcac ggcccgcagc agttcagcct tgccggcaac
     1681 gtcaagttcc tcggctacga aggcctgcag accggcacgg tgcgcgtgac caaggcgggc
     1741 aacccggcct cggcgctgct gctgagctcc gacgtcgaag tggcgccggg cgacctggtt
     1801 ctgccgctcg atgacaaacc ctacgacgcc cagtacgtgc cgcacccgcc gacgaaggtc
     1861 cccgacggca tgcgcgtgat cgcgttcacc gacacgcttg atgcgtccgg cggcatggtc
     1921 accggccgcg accaggtgct ggcgctgtcg cacggcgccg atgacggcat cgagaacggg
     1981 caagtcttct cgatcttcca tccgggcgag aaggtcaacg acaatttcga ctattcaccg
     2041 gaaagcgcga agaagttctt ccatcccaag gacgcgaagg tgcagctgcc cgaggaatac
     2101 gtcggccacg tgatgatctt ccgcacgttc gaacacgtca gctacggcct ggtcgtggac
     2161 gggatcaagc cggtgcacct gggcgacccg ctctacgatc ctgaccacat ctgagcggga
     2221 tcgaggccag gacggccgca tgtccgccgc atccgatgca acccgcacac gcgcctggct
     2281 gaccctgctg cgcgcgccgg ggttgggcgc aggggcaatc cgcacactgc tggccaggca
     2341 cggcgatgtc gtcggcgcgc ttgaacgtgc gcggcgcgaa gcgcccgagg aagcccgcgg
     2401 ctggctcgca gcccccgacc acgcgcggct cgacgccgac gaggcctggt tggcagcagc
     2461 cggccatcat ctgctcagct gcgacaacga cgactttccg ctgctgctgc gcgacatcgg
     2521 cagcgcaccc gcagcgctgt tcgtcagcgg cgatccaacc ctgctgtgga cgccgcagat
     2581 cgcgatcgtc ggcgcgcgca gcgcgagcgc ggcgggcctc gccaacgcgc gcgcgttcgc
     2641 ccgcgccttc gccctcgccg gcaacacgat cacgagcgtg cccagactca gtcccgagat
     2701 gatgcggttg cgccgcggga aatgcgtcgg gtggcccggc acgccgggtg gaaactcgct
     2761 gaccagcgcg ccgtttgcgg cgatgcgcgc ggccagctcg gcatgttggc gcgggtagac
     2821 cagatcgatg ccggtgccga gcacggcgat ggtcttgccg cccgcggcca gcgtcgccgt
     2881 gtgcgccgcg ccgtcgatgc cttcggccag cccgctcgtc accgtgttgc cggcgagggc
     2941 gaaggcgcgt gcgaacgcgc gcgcgttggc gaggcccgcc gcgctcgcgc tgcgcgcgcc
     3001 gacgatggcg atctgcggcg tccacagggc ggtcgggtcg ccgctgacga acagcgccgc
     3061 aggcgcgctg ccgatgtcgc gcagcagcag cggaaagtcg tcgttgtcgc agctgagcag
//
LOCUS       DASRDG010000058         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_707012, whole genome shotgun sequence.
ACCESSION   DASRDG010000058 DASRDG010000000
VERSION     DASRDG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707012"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFA35_00935"
     CDS             complement(<1..208)
                     /locus_tag="VFA35_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZT54761.1"
                     /translation="MKNVTVTMEDSVADWARIEAARRNTSVSRLVGEMLAEKMRHDDA
                     YERAMREALEFKSWGVSDGPYLTRD"
     gene            complement(265..1587)
                     /gene="hisD"
                     /locus_tag="VFA35_00940"
     CDS             complement(265..1587)
                     /gene="hisD"
                     /locus_tag="VFA35_00940"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZT54762.1"
                     /translation="MSASAVNIRLLDTGAEDFEAAFQRVLHWSAETDDAIEQRVAAIL
                     ADVRARGDAAVLEYTARFDGVDAPNVAALEIPRAELLAALSAIPAAQRDALEAAAERV
                     REYHERQLDASGRSWSYRDADGSLLGQKVTPLDRVGIYVPGGKATYPSSVLMNALPAK
                     VAGVGEIIMVVPTPQGVRNAMVLAAAAIAGVNRAFAIGGAQAIGALAYGTTTVPAVHK
                     ITGPGNAYVASAKRRVFGVVGIDMIAGPSEILVLADATTPPEWIAMDLFSQAEHDELA
                     QSILLCPDAGYIAQVKAAIDRLLPAMPRRDVIRASLEGRGALIKTRSMEEACEISNRI
                     APEHLEVSSRDSHRWEPLLRHAGAIFLGAFTSESLGDYCAGPNHVLPTAGTARFSSPL
                     GVYDFQKRSSLIEVSEAGARRLGPIAAELAYGEGLQAHARAAEMRSEL"
     gene            complement(1584..2228)
                     /gene="hisG"
                     /locus_tag="VFA35_00945"
     CDS             complement(1584..2228)
                     /gene="hisG"
                     /locus_tag="VFA35_00945"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZT54763.1"
                     /translation="MITFALPKGRIFDEIVPLLRAAGIEVAEDPEKTRKLIVGTSRPD
                     VRLVLVRASDVPTYVQYGGADLGVVGKDTLLEHGGEGLYQPLDLNIAKCRMSVAVRAD
                     FDYAAAVKQGSRIRVATKYTRIARDHFANKGVHVDLIKLYGSMELAPLTGLADAIVDL
                     VSTGSTLRANHLVEVEQIMNISSRLIVNQAALKLKREPLRGIIDAFERAVGPLP"
     gene            complement(2305..>2726)
                     /locus_tag="VFA35_00950"
     CDS             complement(2305..>2726)
                     /locus_tag="VFA35_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZT54764.1"
                     /translation="GWIRIQSHGRPHPVSFRTSEYPAFPTDMQAQFMALNCIAEGASV
                     VTETIFENRFMHVNELVRLGAKIEVDGHTAVVHGVPQLSGATVMATDLRASASLVIAG
                     LVADGDTIVDRIYHLDRGYDRMEAKLRAIGADIERTK"
BASE COUNT          394 a          950 c          931 g          451 t
ORIGIN      
        1 cgtcgcgggt caggtaaggc ccgtcggaca caccccagct cttgaactcc agcgcctcgc
       61 gcatcgcccg ctcataggcg tcgtcgtgcc gcatcttttc ggccagcatc tcgccgacca
      121 ggcgcgacac gctggtgttg cggcgcgcag cctcgatgcg ggcccagtcg gccacgctgt
      181 cttccatcgt cacggtcacg tttttcatcg cgggcacttc caagccatcc gaaaacacga
      241 ttctcgtgca acacgaactt cgtgtcaaag ctccgagcgc atttctgcgg ctcgcgcgtg
      301 cgcctgcagc ccttcgccgt aggccagctc ggcggcgatc ggcccgagcc tgcgcgcgcc
      361 ggcctcgctg acctcgatca ggctgctgcg cttctggaag tcgtagacgc ccagcggcga
      421 cgagaagcgc gcggtgcccg cggtcggcag cacatggttc ggaccggcgc agtagtcgcc
      481 caggctctcg ctggtgaacg cgccgagaaa gatcgcgccg gcgtggcgca gcagcggctc
      541 ccagcggtgc gagtcgcgcg agctgacttc gaggtgctcg ggcgcgatgc ggttgctgat
      601 ctcgcaggcc tcctccatgc tgcgcgtctt gatcagcgca ccgcggcctt cgagcgaggc
      661 gcggatcacg tcacgccgcg gcatcgccgg aagcagccgg tcgatcgccg ccttcacctg
      721 cgcgatgtag cccgcgtcgg ggcacagcag gatgctctgc gcgagctcgt cgtgttcggc
      781 ctggctgaac aagtccatcg cgatccactc gggcggcgtc gtggcgtcgg ccagcacgag
      841 gatctcgctc ggcccggcga tcatgtcgat gccgacgaca ccgaacaccc ggcgcttcgc
      901 gcttgcgaca tacgcgttgc ccgggccggt gatcttgtgc acggccggca cggtcgtggt
      961 gccgtaggcg agcgcgccga tcgcctgcgc gccgccgatc gcgaacgcgc ggttgacgcc
     1021 ggcgatggcg gccgcggcca gcaccatcgc gttgcgcacg ccttgcggcg tcggcacgac
     1081 catgatgatt tcaccgacac ccgcgacctt cgccggcagc gcgttcatca gcacgctcga
     1141 cggataggtc gccttgccgc ccggcacata gatgccgacg cgatcgagcg gcgtgacctt
     1201 ctggccgagc agcgagccgt ccgcgtcgcg gtagctccac gagcggcccg aggcgtcgag
     1261 ctggcgctcg tggtactcgc gcacacgctc ggccgcggcc tcgagtgcgt cgcgctgcgc
     1321 ggcgggaatc gcgctcaacg ccgccagcag ttcggcccgc gggatttcga gcgcggccac
     1381 gttcggcgcg tcgaccccgt cgaaacgcgc ggtgtattcg agcaccgccg catcgccgcg
     1441 cgcgcgcacg tcggcgagga tcgccgcgac gcgctgctcg atcgcatcgt cggtttcggc
     1501 cgaccagtgc agcacgcgtt ggaacgcggc ctcgaaatct tccgcccccg tgtcgagcag
     1561 gcgaatgttc accgcggatg cactcatggc agcggcccca ccgcccgctc gaaggcgtcg
     1621 atgatgccgc gcagtggctc gcgcttgagc ttcagcgccg cctggttgac gatcaggcgc
     1681 gacgagatgt tcatgatctg ctcgacctcg accaggtggt tcgcgcgcag cgtgctgccg
     1741 gtcgagacga ggtcgacgat cgcgtcggcc agcccggtca gcggcgcgag ctccatcgag
     1801 ccgtagagct tgatcaggtc gacgtgcacg cccttgttcg cgaagtgatc gcgcgcgatc
     1861 cgcgtgtact tggtcgcgac acggatgcgc gagccctgct tcaccgcggc ggcgtaatcg
     1921 aagtcggccc ggaccgcgac gctcatgcgg cacttcgcga tgttcaggtc gagcggctgg
     1981 tacaggccct cgccgccgtg ttcgagcagc gtgtccttgc cgacgacgcc gagatcggcg
     2041 ccgccgtact gcacgtaggt cggcacgtcg gaggcgcgca ccagcacgag ccgcacatcg
     2101 ggccggctgg tgccgacgat cagcttgcgc gtcttctcgg ggtcttcggc gacctcgatg
     2161 ccagcggccc gcagcagcgg cacgatctcg tcgaagatgc ggcccttggg cagtgcgaag
     2221 gtgatcatcg cgcctcccgc cgagccgcct caagggaggc gcgcgcgccc tcggggggcc
     2281 gcgaacgata gtgagctggg ggcttcattt ggttctctcg atgtccgcgc cgatcgcgcg
     2341 cagcttcgct tccatccggt cgtagccacg atcaaggtgg tagatgcgat cgacgatggt
     2401 gtcgccatcg gcgacgagcc cggcgatcac caggctggcc gaggcgcgaa ggtcggtcgc
     2461 catcaccgtc gcgcccgaga gttgcggaac gccgtgcacg accgcggtgt gcccgtcgac
     2521 ctcgatcttc gcgccgagcc gcacgagttc gttgacgtgc atgaagcggt tctcgaagat
     2581 tgtctcggtg acgaccgagg ccccttcggc gatgcagttc agcgccatga actgcgcctg
     2641 catgtcggtc gggaacgcgg ggtactcgct ggtgcgaaag ctcaccgggt gcggacggcc
     2701 gtggctctgg atgcggatcc agccct
//
LOCUS       DASRDG010000059         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_814377, whole genome shotgun sequence.
ACCESSION   DASRDG010000059 DASRDG010000000
VERSION     DASRDG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814377"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..989)
                     /locus_tag="VFA35_00955"
     CDS             complement(12..989)
                     /locus_tag="VFA35_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZT54765.1"
                     /translation="MDALSAVLKALRLETGIFLEAEFTAPWCIDSAPGREVQSLLPRT
                     EHLAIYHLLVQGSCRARLPEDDAEVALAAGDLIMFPHGDGHLLGSDLTRAPVRASGLV
                     QKSPAGGIARIDHGGGGARTRFVCGYLACDWRMCRPMLAALPRMLRVPLGESAGAAWI
                     TSTIQRAAEESRAPRAGTEAVLVKLAELLFVEAIRCHIESIPEDQRSWLAALRDPQVS
                     RALRLLHADPARQWTVDDLGREAGISRSSLAERFALLIGEPPMQYLTRWRLALAARAL
                     QEGDETILRVADEVGYESEAAFNRAFKREFGMPPATWRRQVRRAAAPGG"
     gene            1167..2111
                     /locus_tag="VFA35_00960"
     CDS             1167..2111
                     /locus_tag="VFA35_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54766.1"
                     /translation="MTATASPDRTNTTARYASCIEASKRIRWDIDRDVFRGRRLDFTK
                     KFLPEGLTKARELPFLDAGGQRLLSQIQGRTYANMFGLVERFIGAKMLEVSRDHWLGD
                     QTALEALVRFTDEELKHQELFRRLEVMTAAGMTPGYAFASQPNDVARAVLGKSTWAVL
                     GLTCSIELLTLAHYRQSIEPDAELSELWKDVFTFHWREESQHAILDELEWVRVDRALS
                     AGERDRAVTDLADLVGAVDGILRAQAAADAAYFAAACGRKLRDRETQAVQTTVLRAYR
                     WQYIGSGVTDERFQKILRRMIDERQMARLGEALAPILQ"
BASE COUNT          345 a          818 c          797 g          280 t
ORIGIN      
        1 gcgtgctcgc ctcagccccc cggcgctgcc gcccgccgca cctgccgccg ccaggtcgcc
       61 ggcggcatgc cgaactcgcg cttgaaggcg cggttgaacg ccgcctccga ctcgtagccg
      121 acttcgtcgg cgacgcgcag gatcgtctcg tccccctcct gcagcgcgcg cgcggcgagc
      181 gccagccgcc accgcgtcag gtactgcatc ggcggctcgc cgatcagcag cgcgaaccgc
      241 tcggccagcg acgagcgcga gatgcccgct tcgcgcccca ggtcgtcgac ggtccattgc
      301 cgggccgggt ccgcgtgcag caggcgcagc gcgcggctga cctgcgggtc gcgcagcgcc
      361 gcgagccagc tgcgctggtc ctcggggatg gactcgatgt ggcagcggat ggcttcgacg
      421 aacagcagct ccgcgagctt gacgagcacg gcctcggtgc ccgcgcgcgg cgcgcgcgac
      481 tcctcggcgg cgcgctggat ggtgctggtg atccacgccg cgcccgcact ctcgccgagc
      541 gggacgcgca gcatgcgcgg cagcgcggcg agcatcggcc ggcacatgcg ccagtcgcac
      601 gcgaggtagc cgcagacgaa gcgcgtgcgc gcgccgccgc cgccgtggtc gatgcgcgcg
      661 atcccgcccg ccggcgactt ctgcacgagc cccgaggcgc gcaccggagc gcgcgtgaga
      721 tcgctgccga gcaggtggcc gtcgccgtgc gggaacatga tgaggtcgcc ggcggcgagc
      781 gccacctccg cgtcgtcctc gggcaggcgc gcgcggcacg agccctgcac gagcaggtgg
      841 tagatcgcga ggtgctccgt gcgcggcagc aggctctgca cttccctgcc cggcgccgaa
      901 tcgatgcacc agggcgccgt gaactcggct tcgaggaaga tccccgtctc gaggcgcagc
      961 gccttcagga ctgcggagag cgcgtccacg gcgccacgat caactgcggc ggcgtcccga
     1021 tcaagggacg tgcggcaacg cggcggcgcg cgcaagaggc gcggcgcaga gatcaagtcc
     1081 gcccgccgcg aagtgcctag cgtggttgtc gaacacggcg ccggagtgcc cggcgccgcg
     1141 acggaaacca aaggagacgg accatgatga ctgcaaccgc gtcgcccgac cgcacgaaca
     1201 ccaccgcccg ctatgccagc tgcatcgaag cgtcgaagcg catccgctgg gacatcgacc
     1261 gcgacgtctt ccgcggccgc cgcctcgact tcaccaagaa gttcctgccc gaggggctca
     1321 cgaaggcacg cgagctgccg ttcctggacg ccggcggaca gcgcctgctg agccagatcc
     1381 aggggcgcac ctacgccaac atgttcggtc tcgtcgagcg cttcatcggc gccaagatgc
     1441 tcgaagtgtc gcgcgaccac tggctcggcg accagacggc gctggaggcg ctggtgcgct
     1501 tcacggacga ggaactgaag caccaggagc tgttccgccg cctcgaggtc atgaccgcgg
     1561 cgggcatgac gccgggctat gcattcgcgt cgcagccgaa cgacgtcgcg cgcgccgtgc
     1621 tcggcaagtc gacctgggcg gtgctcgggc tcacgtgcag catcgagctg ctcacgctcg
     1681 cgcactaccg ccagagcatc gagccggacg cggagctgtc ggagctgtgg aaggacgtgt
     1741 tcacgttcca ctggcgcgag gagtcgcagc acgcgatcct cgacgagctg gagtgggtgc
     1801 gcgtggaccg tgcgctctcc gccggcgaac gcgaccgcgc cgtcaccgac ctcgcggacc
     1861 tggtcggggc ggtcgacggc atcctgcgcg cgcaggcggc cgccgacgcg gcgtacttcg
     1921 cggcggcgtg cgggcgcaag ctgcgggacc gcgagaccca ggcggtgcag accacggtgc
     1981 tgcgcgccta ccgctggcag tacatcggct cgggggtcac cgacgagcgc ttccagaaga
     2041 tcctgcgcag gatgatcgac gagcggcaga tggcgcgcct cggcgaggcg ctggcgccga
     2101 tcctgcagta gcgagcgagc aacgaatcca tcttcggccc ccgagaggcg ccgaggactg
     2161 gcgcgccgtc cctagccctt cttcagcagc cagatggtca cgatcaccac cagcgccgac
     2221 gccacccaca gcagcaccac
//
LOCUS       DASRDG010000060         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1515034, whole genome shotgun sequence.
ACCESSION   DASRDG010000060 DASRDG010000000
VERSION     DASRDG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515034"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /gene="rho"
                     /locus_tag="VFA35_00965"
     CDS             complement(<1..1089)
                     /gene="rho"
                     /locus_tag="VFA35_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZT54767.1"
                     /translation="MHLSELKLIHVSELITMGEALEIENVARMRKQELMFAIMKKRAK
                     AGEQVFGDGVLEVLPDGFGFLRSPDASYMASTDDIYLSPSQIRRFNLHTGDAIEGEVR
                     VPKDGERYFALVKVDRVNGVAPEESKHKVMFENLTPLFPKEQFKLEREIKSDENVTSR
                     IIDLIAPIGKGQRALLVAQPKTGKTVMMQQMAHALVANYPEVHLIVLLVDERPEEVTE
                     MVRTVRGEVISSTFDEPAARHVQVAEMVIERAKRLVEMKKDVVILLDSITRLARAYNN
                     VLPSSGKVLTGGMDSNALQRPKRFFGAARNVEEGGSLTIIGTALVDTGSRMDEVIYEE
                     FKGTGNCEIHLDRRMAEKRVYPSILLNKS"
     gene            complement(1319..1648)
                     /gene="trxA"
                     /locus_tag="VFA35_00970"
     CDS             complement(1319..1648)
                     /gene="trxA"
                     /locus_tag="VFA35_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HZT54768.1"
                     /translation="MSSELIKHTTDASFDADVLKSSKPVLVDYWAEWCGPCKMIAPIL
                     DEVSKDYDGRLQIAKMNVDENRDVPAKFGIRGIPTLMLFKNGQLAATKVGALSKAQLT
                     AFLDGHL"
     gene            1760..>3087
                     /locus_tag="VFA35_00975"
     CDS             1760..>3087
                     /locus_tag="VFA35_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54769.1"
                     /translation="MTTIVRAPLENAAGDATRWPAIAARVAAWADEQGLALRDAVVLV
                     PFAQLLPEARRAFARAGGWMPRVETTRTLAASLGPPPPAQAGQLSFDPTLDALSATAL
                     LRSQPWGADWARRDPRGFEQAVRALVATAQALVRGAAALAPAQRASHWAAARELLVPL
                     RGPGASERLLARVALEWAALAPAPATDRLFSAAPPAAWIAIQAGGADPLTARVLADAA
                     VPALVIDTDAADDDPFGQLDAACRPAFAVCVDFEQEAQCAAAQVLAHIERGEAPVALI
                     AQDRVLVRRVRALLERHQLSLLDETGWKLSTTRAAALVMSLLQAARPDASTDALLDWL
                     KAGTDDTAALDALEAACRRDQVARVAGLARVTLEPAAARLWAAASAVLRALGAPRRQP
                     LAAWLSALSSALDASGAMSVLRADAAGEQLIRVLRLGPSAAADGAWQASAA"
BASE COUNT          404 a         1046 c         1107 g          530 t
ORIGIN      
        1 gctcttgttc agcaggatcg acgggtagac gcgcttctcg gccatgcggc gatcgaggtg
       61 gatttcgcag ttgccggtgc ccttgaactc ttcgtagatc acctcgtcca tgcggctgcc
      121 ggtgtcgacc agcgcggtgc cgatgatggt cagcgaaccg ccttcttcga cgttgcgcgc
      181 ggcgccgaag aagcgcttcg ggcgctgcag cgcgttcgag tccatgccgc cggtcagcac
      241 cttgccgctc gagggcagca cgttgttgta ggcgcgggcg aggcgggtga tcgagtcgag
      301 caggatcacc acgtccttct tcatctcgac caggcgcttg gcgcgctcga tcaccatttc
      361 ggcgacctgc acgtggcgcg ccgcgggttc gtcgaaggtc gagctgatga cttcgccgcg
      421 caccgtgcgg accatctcgg tcacttcttc gggccgctcg tcgaccagca gcacgatcag
      481 gtggacctcg gggtagttgg cgaccagcgc gtgcgccatc tgctgcatca tcaccgtctt
      541 gccggtcttg ggctgcgcga cgagcagcgc gcgctggcct ttgccgatcg gtgcgatcag
      601 gtcgatgatg cggctggtga cgttctcgtc ggacttgatc tcgcgttcga gcttgaactg
      661 ctccttcggg aacagcgggg tcaggttctc gaacatgacc ttgtgcttgc tctcctcggg
      721 cgcgacgccg ttcacgcggt cgaccttgac cagcgcgaag tagcgttcac cgtccttggg
      781 cacccgcacc tcgccctcga tcgcgtcgcc ggtgtgcagg ttgaagcggc ggatctggct
      841 cgggctgagg tagatgtcgt ccgtgctcgc catgtagctc gcgtcgggcg agcgcaggaa
      901 gccgaagccg tcgggcagca cctcgagcac gccgtcgccg aacacctgct cgccggcctt
      961 ggcgcgcttc ttcatgatcg cgaacatcag ctcctgcttg cgcatgcggg cgacgttttc
     1021 gatctccagc gcctcgccca tcgtgatgag ctccgagacg tggatcagct tgagttctga
     1081 cagatgcatg ggtggacacc ctgggagtgg taggcgacgg cgcgggtggc cgcatcacgg
     1141 gcgttcggtc gggcattcga gcagcgggtg cgctcgatca acgaacggaa caacgagaga
     1201 actggagtgc ttgtgccgaa tccacgctca ggggcatggt ggcgaatctc ttggtgcgga
     1261 agtcggcggc ctggctacct ggtgggtaag gggccgaccc ccgcagcgtt tgccgagatt
     1321 atagatgacc gtcgaggaag gcggtcaatt gggctttcga gagggcgccg accttggtcg
     1381 ccgcgagctg gccgttcttg aacagcatca gcgtcggaat gccgcggatg ccgaacttgg
     1441 ccggcacgtc gcgattttcg tcgacgttca tcttggcgat ctgcaggcga ccgtcgtagt
     1501 ccttcgagac ctcgtcgagg atcggcgcaa tcatcttgca cgggccgcac cactcggccc
     1561 agtagtcgac cagcaccggc ttgctggact tcagcacgtc ggcgtcgaac gaggcgtcgg
     1621 tggtgtgttt gatcagttca ctgctcatgg ttcgtccttc tgtctgcacc gctcgagttg
     1681 ggtggcaagg cacaatgatt ctgacacaaa ggcctcgctg ccgcggggcg tcgggccgtt
     1741 cacgaccctc ttcgttccta tgacgaccat agtgcgggct ccccttgaaa acgcggccgg
     1801 cgacgccacg cgctggccgg cgatcgccgc gcgcgtggcc gcctgggcgg acgagcaggg
     1861 ccttgcgctg cgcgacgccg tcgtgctcgt gccgttcgcg cagctgctgc ccgaggcgcg
     1921 ccgcgcgttc gcccgcgccg gcggctggat gcccagggtc gagaccaccc gcacgctcgc
     1981 cgcgagcctc ggcccgccgc cgccggcgca ggccgggcag ctcagcttcg atccgacgct
     2041 ggatgcgctc agcgcgaccg cgctgctgcg ttcgcagccc tggggcgcgg actgggcgcg
     2101 gcgcgacccg cgcggcttcg agcaggcggt gcgcgcgctg gtcgcgacgg cgcaggcgct
     2161 ggtgcgcggc gcggccgcgc tggcgccggc acagcgtgct tcgcactggg cggcggcacg
     2221 cgagctgctc gtgccgctgc gcggcccggg cgcgagcgag cggctgctgg cgcgtgtcgc
     2281 gctcgagtgg gccgcgctgg cgcccgcgcc ggcgaccgac cggctcttca gcgccgcgcc
     2341 gccggccgcg tggatcgcga tccaggccgg cggcgccgac ccgctgactg cgcgcgtgct
     2401 cgccgacgcg gccgtgcccg cgctcgtgat cgacaccgac gccgcggacg acgatccctt
     2461 cggccagctc gatgccgcgt gccgacccgc cttcgcggtt tgtgtcgact tcgagcagga
     2521 ggcgcagtgc gccgccgcgc aagtgctcgc gcacatcgaa cgcggcgagg cgccggtcgc
     2581 gctgatcgca caggaccgcg tgctggtgcg gcgcgtgcgc gcgctgctcg agcggcacca
     2641 attgagcctg ctcgacgaaa ccggctggaa gctctcgacc acgcgcgccg cggcgctggt
     2701 gatgagcctg ctgcaggccg cgcggcccga cgcgagcacc gatgcactgc tcgactggct
     2761 gaaggctggc accgacgaca cggccgcgct cgatgcgctc gaagccgcgt gccggcgcga
     2821 tcaggtggcg cgcgtcgccg ggctcgcgcg ggtcacgctc gagccagctg cggcgcgctt
     2881 gtgggccgcg gcgtcggcgg tgttgcgggc actcggggcg ccgcgccgtc aaccgctcgc
     2941 ggcgtggctg agtgctctgt cgagcgcact cgacgccagc ggcgcgatgt ccgtgctgcg
     3001 cgccgacgcc gctggcgaac agctgatcag ggtcttgcgg ctcggcccga gcgccgccgc
     3061 cgatggcgcc tggcaggcca gcgcggc
//
LOCUS       DASRDG010000061         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_707288, whole genome shotgun sequence.
ACCESSION   DASRDG010000061 DASRDG010000000
VERSION     DASRDG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707288"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VFA35_00980"
     CDS             <1..1114
                     /locus_tag="VFA35_00980"
                     /inference="COORDINATES: protein motif:HMM:NF044767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZT54770.1"
                     /translation="QLAQRITARYHLTPLDRDETEAYVRHRLAIAGALRSPFSRLGLR
                     ALYKRSGGVPRLINVIGDRALMAGYAREQNSIGERLVDRAADETLPGTTRYRLRHYGP
                     WAAAACAVIALGAAAYFHQLQKPVPAPTPTAPPPPVAPAAASGAATTTAAAAEPAKPQ
                     ENPAGKKLAALVENAADSELTAWTQLLARWQATSAETSVRDAARCHAVVFPGMNCLRG
                     HGAITQLARFDRPLILPIKAQNASAYALLQGAAGDQVQLNLGGESFRVGKDDLASVWD
                     GEFLALWRLPADVPARLRSGDAGPGVAWVKAQLARMDGGAADAGPAFFDDALAERVRK
                     LQTAYGIKPDGIVGPETLFALSALDPGGPHLTRSVQ"
     gene            1722..1922
                     /locus_tag="VFA35_00985"
     CDS             1722..1922
                     /locus_tag="VFA35_00985"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HZT54771.1"
                     /translation="MRSALPALPITMQVYSADPKRRFAIIEGTRVTEGESVRGVTIVE
                     IRASGLVLEFHGHRMLMPRPGS"
     gene            1928..>2119
                     /locus_tag="VFA35_00990"
     CDS             1928..>2119
                     /locus_tag="VFA35_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54772.1"
                     /translation="MFVTVDRQRQSSLPWATLAILAACVTLFLWLVLAPDATRWQFLR
                     QWGTVPTRLFDANAPVLQQL"
BASE COUNT          325 a          795 c          707 g          292 t
ORIGIN      
        1 ccagctcgct cagcgcatca ccgcgcgcta tcacctgacg ccgctcgacc gcgacgagac
       61 cgaagcctac gtacgccatc ggctcgcgat cgccggcgcg ttgcgttcgc cattctcgcg
      121 tcttggtctg cgcgcgctgt acaagcgctc cggcggcgtg ccgcgcctga tcaacgttat
      181 cggcgaccgc gcgctgatgg ccggctacgc gcgcgaacag aattcgatcg gcgagcgcct
      241 cgtcgaccgc gccgccgacg aaacgctgcc cggcacgacg cgctaccgcc tgcgccacta
      301 cggcccgtgg gccgcggccg cgtgtgcggt gatcgcactc ggcgcggcgg cgtatttcca
      361 tcagttgcag aagcccgtgc ctgcgccgac gcccaccgcg ccaccgccgc cagtcgcacc
      421 ggccgccgca agcggagccg cgacgacgac ggcggccgcg gccgagccgg cgaagccgca
      481 ggaaaatccg gccgggaaga aacttgccgc gctcgtcgag aatgctgccg attccgaact
      541 gaccgcgtgg acgcagttgc tcgcgcgctg gcaggcgacc tcggccgaaa cctcggtgcg
      601 cgacgcggcg cgttgccatg ccgtggtctt tcccggcatg aactgcctgc gcggacacgg
      661 tgcgatcacg cagctcgcgc gcttcgaccg gccgctgatc ctgccgatca aggcgcagaa
      721 cgcgagcgcg tatgcgctgc tgcagggcgc ggccggcgac caggtacagc tcaatctcgg
      781 cggcgagagt tttcgcgtcg gcaaggacga tctcgccagc gtgtgggacg gcgagttcct
      841 tgcgctgtgg cgcctgccgg cggatgtgcc ggcccggttg cgcagcggcg acgccggccc
      901 gggcgtggcc tgggtcaagg cccagcttgc gcgcatggac ggcggcgcgg ccgatgccgg
      961 ccccgcgttc ttcgacgacg cgctcgccga gcgcgtgcgc aagctgcaga ccgcctacgg
     1021 catcaagccc gacggcatcg tcggcccgga aacgctgttt gcactgtccg cgctcgaccc
     1081 aggcggcccg cacctgaccc gcagcgtgca gtgaacgcgc acggcatttt gcgcaaaaag
     1141 ataaaaatat acccgttgac gaatccatgt cgctgatcct cgaagccctg aagaagtccg
     1201 aacagcagcg ccggctcggc gagatgccaa acctcggcac gccggtcacg gcgacacggc
     1261 ggcggcgcag cccgctgccg tggctggcgg tggcggtcgt gctcgcgctg ggcgcggccg
     1321 gcgcctggcg ctacctgcgc cagcacgcgg cgcagacacc ggtcgcggcg acaggtgatt
     1381 cggcgacggt caaggcgacg acgaatacag cgaccgagcg cgcgatcacg agcgtgccgc
     1441 agccagcgcc gccaaggccg gtcgcaccgg tggtggcaca gaccattccc gcggcgccac
     1501 cgagaaagga atcgacaagc ccgacgccag caccggcagt cacacgtgca ccggcaccgt
     1561 cgcccgcgcc ggttgccaca cctgtgcctg cagcaccccc tgctcccgaa cctgcatcgg
     1621 cgactccggc tccggcaatc gcgccaccgc ctgcgcctgc cgctgcggcg cccgctcccg
     1681 ccgcaaacga tgtgcccacg gtcgatgaaa tgcccgcgga cgtgcgcagc gcgctgcccg
     1741 cgctgccgat cacgatgcag gtctactcgg ccgatcccaa gcgccgcttc gccatcatcg
     1801 aaggaacgcg cgtgaccgag ggcgagagcg tgcgcggcgt gaccatagtc gagattcgcg
     1861 ccagcgggct tgtgctcgaa ttccacggcc accgcatgct gatgccgcga ccgggttcct
     1921 gagcgccgtg ttcgtcaccg tcgaccgcca gcgccaatcg tccctgccct gggccacgct
     1981 cgcgatcctc gccgcctgcg tcacgctgtt cctctggctc gtgctcgcgc ccgacgcgac
     2041 gcgctggcag ttcctgcgcc agtggggcac cgtgccgacg cgtctgttcg acgccaacgc
     2101 accggtgctg cagcagctc
//
LOCUS       DASRDG010000062         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1620547, whole genome shotgun sequence.
ACCESSION   DASRDG010000062 DASRDG010000000
VERSION     DASRDG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620547"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /gene="xdhB"
                     /locus_tag="VFA35_00995"
     CDS             complement(<1..2287)
                     /gene="xdhB"
                     /locus_tag="VFA35_00995"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HZT54773.1"
                     /translation="MNKPIDAPLRQAAEPFADHSRNAAARIDAGAEAAAAAAGARVGI
                     SRPHESAQLHVAGEATYIDDIPEIKGTLHCALGLAPVANGRLTALALAPIRALPGVVA
                     VLTAADIPGPNDCGPIIHDDPILCDIVKHDDGSSWGEIRHLGQPVFAVIAETRDIARR
                     AAAQAKDVLTIESAAPVITPQQAHLLRQYVLPPMHLVRATNGGAQAAISAAPHRLKGT
                     LEVGGQEQFYLEGQISYAVPTEGGGMHVHCSTQHPSEMQHLVARALKLDAHDVRVECR
                     RMGGGFGGKESQSALFACVAAVAAARCKRPVKLRLDRDDDFLITGRRHCFWYEYEAGY
                     DDAGRVLGVEVAMVSRAGHSADLSGPVMTRALCHFDNAYWLPDVAMHGYSGKTNTQSN
                     TAFRGFGGPQGAIAIENILDTIARALGRDPMDVRRANFYGKDTNNVTPYGQLVSDNII
                     HELTAELEASSDYRARRAAIAQFNATSPVLKRGIALAPLKFGISFNVKHFNQAGALVH
                     VYNDGSILVNHGGTEMGQGLNTKVAQVVAHELGVSFERVRVTATDTQKVANTSATAAS
                     TGADLNGKAAQDAARQIRERLAACIASHHGGKPEDVRFANDKVEVNGKSLPFSAVVAQ
                     GYLDRVQLWSDGFYATPGLSWDKDKMFGRPFYYFAYGAAVSEVVIDTLTGEWKLLRAD
                     ILHDAGRSLNPAVDIGQVEGAFIQGMGWLTTEELVWHPQSGLLTTHAPSTYKIPTAND
                     CPPVFNVQLFDGRNVEDSIHRS"
BASE COUNT          308 a          790 c          870 g          415 t
ORIGIN      
        1 tgctgcggtg gatgctgtct tcgacattgc ggccgtcgaa gagctgcacg ttgaagaccg
       61 gtgggcagtc gttcgcggtc gggatcttgt aggtgctcgg tgcatgcgtc gtcagcaggc
      121 ccgactgtgg gtgccacacg agctcttcgg tcgtcagcca gcccatgccc tggatgaacg
      181 cgccttcgac ctggccgatg tcgacggccg ggttcagcga gcggccggcg tcgtgcagga
      241 tgtcggcgcg cagcagcttc cactcgccgg tcaaggtgtc gatgacgact tcgctgaccg
      301 cggcgccgta cgcgaaatag tagaacggcc ggccgaacat cttgtccttg tcccagctca
      361 ggcccggcgt cgcgtagaag ccgtcggacc agagctgcac ccggtcgagg tagccctgcg
      421 cgacgacggc gctgaacggc agtgatttgc cattgacctc gaccttgtcg ttcgcgaagc
      481 gcacgtcttc cggcttgccg ccgtggtgcg acgcgatgca ggccgcgagc cgctcgcgga
      541 tctggcgcgc cgcgtcctgc gctgccttgc cgttcaggtc ggcgccggtc gacgcggcgg
      601 tggccgaggt gttcgcgacc ttctgcgtgt cggtcgcggt cacgcgcacg cgctcgaagc
      661 tcacgccgag ctcgtgcgcg acgacctgcg cgaccttggt gttcagaccc tggcccatct
      721 cggtgccgcc gtggttcacg aggatcgagc cgtcgttgta gacgtgcacc agcgcgccgg
      781 cctggttgaa gtgcttgacg ttgaacgaga tgccgaactt cagcggcgcc agcgcgatgc
      841 cgcgcttgag caccgggctg gtcgcgttga actgcgcgat cgcggcgcgg cgcgcgcggt
      901 agtcgctgct cgcctccaac tcggcggtga gttcgtggat gatgttgtcg ctgacgagct
      961 gaccatatgg cgtgacgttg ttcgtgtctt tgccgtagaa gttcgcgcgg cgcacgtcca
     1021 tcgggtcgcg gcccagcgcg cgcgcgatcg tgtcgaggat gttctcgatc gcgatcgcgc
     1081 cctgtggacc gccgaagccg cggaacgcgg tgttgctctg ggtgttggtc ttgcccgaat
     1141 agccgtgcat cgccacgtca ggcagccagt aggcgttgtc gaagtggcac agcgcgcgcg
     1201 tcatcaccgg gcccgacagg tcggccgagt ggccggcgcg cgagaccatc gcgacctcga
     1261 cgccgagcac gcggccggcg tcgtcgtagc cggcctcgta ctcgtaccag aagcagtggc
     1321 gccggccggt gatcaggaag tcgtcgtcgc ggtcgagccg cagcttgacc gggcgcttgc
     1381 atctggccgc cgccactgcc gcgacgcacg cgaacagcgc cgactgcgac tccttgccgc
     1441 cgaagccgcc gcccatgcgc cggcactcga cccgcacgtc gtgcgcgtcc agcttcagcg
     1501 cgcgcgcgac caggtgctgc atctcgctcg ggtgctgggt cgagcagtgg acgtgcatgc
     1561 cgccgccttc ggtcggcacc gcgtagctga tctggccttc gaggtagaac tgctcctggc
     1621 cgccgacctc gagcgtgccc ttcagccggt gcggcgcggc cgagatcgcg gcctgcgcgc
     1681 caccgttcgt cgcgcgcacc aggtgcatcg gcggcagcac gtactggcgc agcaagtgcg
     1741 cctgctgcgg tgtgatgacc ggcgcggcgg attcgatcgt cagcacgtcc ttggcctggg
     1801 ctgcggcacg gcgggcgatg tcgcgggttt cggcgatcac cgcgaacacc ggttggccga
     1861 gatggcggat ctcgccccac gacgagccat cgtcgtgctt cacgatgtcg cacagaatcg
     1921 ggtcgtcgtg gatgatgggg ccgcagtcgt tcggaccggg gatgtcggcg gccgtcagca
     1981 ccgcgacgac gccgggcagc gcgcggatcg gcgccagcgc cagcgccgtc agccggccgt
     2041 tcgcgaccgg cgcgaggccg agcgcgcagt gcagcgtgcc tttgatctcg gggatgtcgt
     2101 cgatgtaggt cgcctcgccg gccacgtgca gctgtgccga ttcgtgcggg cggctgatgc
     2161 ccacgcgcgc gccggccgcg gcggcggcgg cttcggcgcc ggcgtcgatg cgcgcggcgg
     2221 cgttgcgcga gtggtcggcg aagggctcgg cggcttgccg cagcggggcg tcgatcggct
     2281 tgttcatcgt cttgcccttc aggcggtggc gtgcggcatc acgctgaaca cgctggtcaa
     2341 ggggctcgcg agcgggttct cggcgcgggt ttcgagccac agg
//
LOCUS       DASRDG010000063         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_707373, whole genome shotgun sequence.
ACCESSION   DASRDG010000063 DASRDG010000000
VERSION     DASRDG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707373"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFA35_01000"
     CDS             <1..939
                     /locus_tag="VFA35_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZT54774.1"
                     /translation="NLFLAWVLVRYRHRAGHRAAYQADNRRLERWLIVGTTVGIAALL
                     APGLVVYAEYVTAPGDALVLEVLGQQWKWSYRLPGADARFGGVDPRFVSATNPFGLDP
                     GDPAGADDLLVPGDEVHVPLGRPVKVLLRSNDVLHDFYVPPFRARMNIVPGTVTSFWF
                     TPTQTGRFEVLCAQLCGVGHYNMRGFVVVEPAAAFAGWVAHQTSFAQALNAAASVDPL
                     VARGRALAQSKGCVACHTVDGSKGVGPTWQGLLGKTEHFTDGSSARLDADALRQDIRE
                     PLARIAQGYAAVMPRPELSDAELDALVAYIGAQSPK"
     gene            946..2718
                     /gene="ctaD"
                     /locus_tag="VFA35_01005"
     CDS             946..2718
                     /gene="ctaD"
                     /locus_tag="VFA35_01005"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZT54775.1"
                     /translation="MAQTLHADAALAAHAPTSFVARYVWSQDHKVIAVQYSAIAVFTG
                     LVGLVLSDLMRLQIGFPGRFAFIDAAHYYQFVTMHGMIMVIYLLTALFLGGFGNYLIP
                     LMVGARDMAFPYLNMLSVWVFLLAVIVLMASFFAPGGPTGAGWTLYPPQVIMPGTPGH
                     DWGIILMLVSLAIFIVATTMGGLNYVTTVLQLRCEGMTLMRMPLTVWGIFVATILALL
                     AFPALFVSAVMLLLDKTLGSSFFMPAMVSLGQATNYRGGSPLLFQHLFWFFGHPEVYI
                     IALPAFGVVSDLISVHARKAIFGYRMMVWAILAIGVLSVVVWAHHMFVSGMNPYFGFF
                     FATTTLVIAVPTAIKVYNWLLTLWRGDIHLSVPMLFALAFISTFIIGGLTGLFLGNVS
                     VDIPLSGTYFVVAHFHMVMAVSPILVVFGGIYHWYPKVTGRMLDDRLGLAHFWITFIG
                     TYAIYFPMHYLGILGMPRRYYAYEGYQFIPASAQSLNAFITVVALIVGVAQLLFIANL
                     VWSWRHGAKADPNPWRAASLEWQTPQTPPAHLNWGERQPVAYRWAYAYSVPGAAEDFI
                     AQNAPPQAGGDEPGQGALRPGGAA"
     gene            2715..3305
                     /locus_tag="VFA35_01010"
     CDS             2715..3305
                     /locus_tag="VFA35_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bb3-type cytochrome oxidase subunit III"
                     /protein_id="HZT54776.1"
                     /translation="MSTTTLTRTTPPASRSDAGVALWVFIGVATTLFALFIMAYVMRM
                     AGSDWSSIALPWQLGLATALLAAGSAAMALAARAAQRADGTRARAMLLAGGACAAAFL
                     GAQLWGWAALLEAHVLVNGNPAASFFYLLTAMHGLHVIGGLIAWGFATRAPAAIVLCA
                     RYWHFLLAVWLVLLATLAGVTPAFVAWACGPLGVVR"
     gene            3302..3982
                     /locus_tag="VFA35_01015"
     CDS             3302..3982
                     /locus_tag="VFA35_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III family protein"
                     /protein_id="HZT54777.1"
                     /translation="MSAVMTAPSHPSAGWRGLVADWSSDQQAFQVSWAKAMMWIFLLS
                     DTFVFSCFLTGYMTVRTGSAVPWPNASHVFSLRIGGSELPLILIAIMTFVLITSSGTM
                     AMAVNFAYRRDRVNAATLMILTAALGETFVGMQAFEWTKLILEGVRPWGNPMGAPQFG
                     SSFFMITGFHGLHVTVGVIYLLTVATRLLRGKYERSANYQIVEIAGLYWHFVDLVWVF
                     IFALFYLW"
     gene            3991..>4185
                     /locus_tag="VFA35_01020"
     CDS             3991..>4185
                     /locus_tag="VFA35_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV"
                     /protein_id="HZT54778.1"
                     /translation="MEARLHQQHQQHQQHPIGLYLKVWGALFVLSTMSYLVDVFQFQG
                     LLRWGLIIVLMLAKAGLIVAV"
BASE COUNT          550 a         1462 c         1410 g          763 t
ORIGIN      
        1 aatctgttcc tggcctgggt gctggtgcgc tatcgccacc gcgcaggcca ccgcgccgcg
       61 taccaggccg acaaccgcag gctcgaacgc tggctcatcg tcggcacgac cgtgggcatc
      121 gcggcgctgc tcgcgcccgg cctcgtggtc tacgccgagt acgtgaccgc ccccggcgat
      181 gcgctcgtgc tggaggtgct cggccagcaa tggaagtgga gctaccggct gcccggcgcc
      241 gacgcgcgct tcggtggtgt cgacccgcgt ttcgtctcgg cgacgaaccc gttcggcctc
      301 gaccccggcg acccggccgg ggccgacgac ctgctggtgc cgggcgatga agtgcatgtg
      361 ccgctgggcc gcccggtcaa ggtgctgctg cgctccaacg acgtgctgca cgacttctac
      421 gtgccgccgt ttcgcgcccg catgaacatc gtccccggca ccgtcacgtc gttctggttc
      481 acgccgacgc aaaccggccg cttcgaggtg ctgtgcgcgc agctgtgcgg cgtcggccac
      541 tacaacatgc gcggcttcgt cgtggtcgag ccggccgcgg cgtttgcagg atgggtcgcg
      601 caccagacga gcttcgcgca ggcgctgaac gccgccgcga gcgtcgaccc gctggtcgcg
      661 cgcgggcgtg cgctggccca gtcgaagggc tgcgtcgcgt gccacacggt agacggcagc
      721 aagggggtcg gcccgacctg gcaaggcctg ctcggcaaga ccgagcactt caccgacggc
      781 agcagcgcgc ggctggatgc ggacgcgctg cgccaggaca tccgcgagcc gctcgcgcgc
      841 atcgcgcagg gctatgcggc ggtgatgccc cggcccgagt tgagcgacgc cgagctcgat
      901 gcgctggtcg cctacatcgg cgcccagtcg ccgaagtgag ccgcgatggc ccagaccctt
      961 catgccgacg ccgcgctggc cgcgcatgcg ccgacgagct tcgtcgcgcg ctatgtctgg
     1021 agccaggacc acaaggtcat cgcggtgcag tacagcgcca tcgcggtctt caccgggctg
     1081 gtcgggctcg tgctgtcgga cctgatgcgg ctgcagatcg gttttccggg ccgcttcgcg
     1141 ttcatcgacg cggcgcacta ctaccagttc gtcacgatgc acgggatgat catggtgatc
     1201 tacctgctca ccgcgctgtt cctcggcggc ttcggcaact acctgatccc gctgatggtg
     1261 ggcgcgcgcg acatggcgtt cccgtacctg aacatgctca gcgtctgggt cttcctgctc
     1321 gcggtgatcg tgctgatggc gagcttcttc gcgcccggcg gccccaccgg cgcgggctgg
     1381 acgctgtacc cgccgcaggt catcatgccc ggcacgccgg ggcacgactg gggcatcatc
     1441 ctgatgctgg tttcgctcgc gatcttcatc gtcgcgacga cgatgggcgg cctgaactac
     1501 gtcaccaccg tgctgcagct gcgctgcgag ggcatgacgc tgatgcgcat gccgctcacc
     1561 gtgtggggca tcttcgtcgc gaccatcctc gcgctgctcg cgttcccggc gctgttcgtc
     1621 agcgccgtga tgctgttgct cgacaagacc ttgggcagca gcttcttcat gccggcgatg
     1681 gtctcgctcg gccaggcgac gaactaccgg ggcggcagcc cgctgctgtt ccagcacctg
     1741 ttctggttct tcggccatcc cgaggtctac atcatcgcgc tgcccgcctt cggcgtcgtc
     1801 tcggacctga tcagcgtgca cgcgcgcaag gcgatcttcg gctaccggat gatggtctgg
     1861 gcgatcctgg cgatcggcgt gctcagcgtc gtcgtctggg cgcaccacat gttcgtcagc
     1921 ggcatgaacc cgtacttcgg cttcttcttc gcgaccacga cgctcgtcat cgccgtgccc
     1981 accgcgatca aggtctacaa ctggctgctg accctgtggc gcggcgacat acacctgtcg
     2041 gtgccgatgc tgttcgcgct cgcgttcatc agcaccttca tcatcggcgg gctgaccggg
     2101 ctgttcctcg gcaacgtcag cgtcgacatc ccgctgtcgg gcacctactt cgtcgtcgcg
     2161 cacttccaca tggtgatggc ggtgtcgccg atcctggtcg tgttcggcgg catctaccac
     2221 tggtacccca aggtcaccgg ccggatgctc gacgaccggc tcggcctggc gcacttctgg
     2281 atcaccttca tcggcacgta tgcgatctac ttcccgatgc actacctcgg catcctgggc
     2341 atgccgcggc gctactacgc ctacgagggc taccagttca tcccggcctc ggcgcaatcg
     2401 ctgaacgcgt tcatcacggt cgtggcgctg atcgtcggcg tcgcgcagct gctgttcatc
     2461 gcgaacctgg tctggagctg gcgccacggc gcgaaggccg accccaaccc gtggcgcgcc
     2521 gcttcgctcg aatggcagac gccgcaaacg ccgccggcgc acctgaactg gggtgagcgg
     2581 cagccggtcg cgtaccgctg ggcctatgcg tacagcgtgc cgggggcggc cgaggacttc
     2641 atcgcgcaga acgcgccgcc gcaggccggc ggcgacgagc ccgggcaagg cgcgctgcgg
     2701 ccgggaggcg cggcgtgagc acgacgacgc tgacgcgcac caccccgccg gcatcgcgct
     2761 ccgacgccgg cgtcgcgctg tgggtcttca tcggcgtcgc gacgaccttg ttcgcgctgt
     2821 tcatcatggc ctacgtgatg cgcatggcgg gcagcgactg gtcgtcgatc gcgctgccct
     2881 ggcagctcgg gctcgcgacc gcgctgctcg cggccggcag cgcggcgatg gcgctcgcgg
     2941 cgcgggccgc gcagcgtgcg gacggcacgc gcgcacgcgc catgctgctc gccggcggcg
     3001 cctgcgccgc ggcctttctc ggcgcgcagc tgtggggctg ggccgcgctg ctggaggcgc
     3061 acgtgctcgt gaacggcaac ccggcggcga gcttcttcta cctgctcacc gcgatgcacg
     3121 gcctgcacgt gatcggcggc ctgatcgcct ggggcttcgc gacccgcgcg cccgcggcga
     3181 tcgtgctgtg cgcgcgctat tggcactttc tgctcgcggt ctggctcgtg ctgctcgcga
     3241 cgctggccgg cgtgacgccc gcattcgtcg cctgggcgtg cgggccgttg ggggtggtgc
     3301 gatgagcgcc gtcatgacgg cgccgagcca cccgtcggcc ggctggcgcg gtctggtcgc
     3361 cgactggtcc tcggaccagc aggcgttcca ggtctcgtgg gccaaggcga tgatgtggat
     3421 cttcctgctc agcgacacct tcgtcttcag ctgcttcctg accggctaca tgacggtgcg
     3481 caccggctcg gccgtgccct ggccgaacgc cagccacgtg ttctcgctgc gcatcggcgg
     3541 cagcgagttg ccgctgatcc tgatcgcgat catgaccttc gtgctgatca ccagcagcgg
     3601 cacgatggcg atggccgtca acttcgccta ccggcgcgac cgcgtcaacg cggcgacgct
     3661 gatgatcctg accgccgccc tgggcgagac cttcgtcggc atgcaggcct tcgagtggac
     3721 caagctgatc ctcgagggtg tgcgcccctg gggcaacccg atgggcgcgc cgcagttcgg
     3781 ctccagcttc ttcatgatca ccggctttca cgggctgcac gtgacggtcg gcgtgatcta
     3841 cctgctgacg gtggcgacgc gccttttgcg cggcaagtac gagcgcagcg ccaactacca
     3901 gatcgtcgag atcgccgggc tgtactggca cttcgtcgac ctcgtctggg tcttcatctt
     3961 cgcgctgttc tacctgtggt gaggtgagcg atggaagcac ggctgcatca gcaacaccag
     4021 caacatcagc aacacccgat tggcctgtac ctgaaggtct ggggcgcgct gttcgtgctc
     4081 agcacgatgt cgtacctggt cgacgtgttc cagttccagg gcctgctgcg ctggggcctg
     4141 atcatcgtgc tgatgctggc caaggccggg ttgatcgtcg cggtc
//
LOCUS       DASRDG010000064         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_125347, whole genome shotgun sequence.
ACCESSION   DASRDG010000064 DASRDG010000000
VERSION     DASRDG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125347"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFA35_01025"
     CDS             complement(<1..589)
                     /locus_tag="VFA35_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HZT54779.1"
                     /translation="MRSSSSSKKSGLAGLVLAAVGVVYGDIGTSPLYALKEVFAHGHV
                     MLTPQNIYGVLSLVFWTLTVVVSVKYVVLILRADNNGEGGLIAMLALASSAVRGRPAL
                     RRRLLLLGIFGTAIFFGDGVITPAISVLSAVEGLEVAAPALHAYVIPVTLMVLTALFM
                     VQRHGTARVGKAFGPVMVLWFGVLAVLGLVHIAENP"
     gene            complement(635..2509)
                     /locus_tag="VFA35_01030"
     CDS             complement(635..2509)
                     /locus_tag="VFA35_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HZT54780.1"
                     /translation="MSRKSGTSSLAALTLGALGVVYGDIGTSPLYALREVFATKHASL
                     SQPDILGVLSLVFWTLTIIVSLKYVVLIMRANNNGEGGTMALMALSSHATKDRPRMRS
                     ALIVMGILGAALFYGDGVITPAISVLSAVEGLEVVTPAFKPYVVPITLAVITVLYAVQ
                     RFGTGTMGRWFGPFTVVWFLVIGFWGVVWIVRAPQVLGALNPWHAISFGIEHGHVTLL
                     VLGAVFLAVTGAEALYADMGHFGSRPIRIAWFVLVFPALVLNYFGQGALMLVEPGKAD
                     NPFFNMVPGWFLIPMVVMATLATVIASQATISGTFSVTKQAIQLGYLPRLRVLHTSVR
                     DTGQIYIPGVNWLQYAAIVAAVLHFGSSSALATAYGIAVTATMVLTTVMTFFVIRYRW
                     NYPLWMCVGATGFFLAIELVFFASNILKVLSGGWFTLLIAAFVFTVMMTWARGVELLS
                     ASLRADAIELKPFLKSVLADPPMRAPGTAVFMNGEADLTPAALLHNLKHNNVLHETNL
                     FVTVDTLDVPWVPFAERARAEPLCKGCWSVTLNFGFKNDVDVPAALELLRGRGFELDP
                     MTTSYFLSRSTVVPTLGGGMAPWREKLFANMHRNAAAAADFLNLPPNRVVELGTKVRI
                     "
     gene            complement(2647..>2861)
                     /locus_tag="VFA35_01035"
     CDS             complement(2647..>2861)
                     /locus_tag="VFA35_01035"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZT54781.1"
                     /translation="ERGIDENLNAPGAHFAPLAFAEHNQLPKPGAKPGASAPNRFYMY
                     GVIARLAMVAASYELEATDPDAEVYD"
BASE COUNT          519 a          969 c          975 g          398 t
ORIGIN      
        1 aagggttctc ggcgatgtgc acgaggccga gcacggccag caccccgaac cacagcacca
       61 tcaccggccc gaaggccttg ccgacgcggg cggtgccgtg gcgctgcacc atgaacagcg
      121 ccgtcagcac catcagcgtc actgggatca cgtaggcgtg cagcgccggc gccgccactt
      181 cgaggccttc gaccgccgag agcaccgaga tcgccggcgt gatcacgccg tcgccgaaga
      241 agatcgcggt gccgaagatg cccagcagca gcaggcgccg gcgcagcgcg ggccggccgc
      301 gcaccgccga cgacgccagc gccagcatcg cgatcagacc gccctcgccg ttgttgtcgg
      361 cgcgcaggat cagcaccacg tacttgaccg agacgaccac cgtcagcgtc cagaacacca
      421 gcgacagcac gccgtagatg ttctggggcg tgagcatcac gtggccgtgc gcgaagactt
      481 ccttcagcgc atacagcggg ctggtgccga tgtcgccgta gacgacgccg acggccgcga
      541 gcaccagccc ggccaggccc gacttcttgg acgacgagga ggaacgcacg cgcactgccg
      601 gaaattcttg tcgggcgatt gtgcgcccga gtgtctagat tcggactttc gtgccgagct
      661 cgaccacccg gttcgggggc aggttcagga agtcggcggc cgcggcggcg ttgcggtgca
      721 tgttcgcgaa cagcttctcg cgccacggcg ccatgccgcc gccgagcgtg ggcacgacgg
      781 tcgagcgcga caggaaatac gaggtcgtca tcggatcgag ctcgaagccg cgcccgcgca
      841 gcagttcgag tgcggcgggc acgtcgacgt cgttcttgaa gccgaagttc agcgtcaccg
      901 accaacagcc cttgcacagc ggctcggcgc gggcgcgctc ggcgaacggc acccacggca
      961 cgtcgagcgt gtcgaccgtg acgaacaggt tggtctcgtg caacacattg ttgtgcttca
     1021 ggttgtgcag cagcgcggcc ggcgtcaggt cggcttcgcc gttcatgaac accgcggtgc
     1081 cgggcgcacg catcggcggg tccgcgagca cgctcttcag gaagggcttg agctcgatcg
     1141 cgtcggcccg caggctcgcc gacagcagct cgacaccacg cgcccaggtc atcatcacgg
     1201 tgaacacgaa ggccgcgatc agcagcgtga accagccgcc cgacagcacc ttcaggatgt
     1261 tcgacgcgaa gaacacgagc tcgatcgcga ggaagaagcc ggtcgcgccg acacacatcc
     1321 acaacgggta gttccagcgg tagcggatca cgaagaaggt catcaccgtc gtcagcacca
     1381 tcgtcgcggt cacggcgatg ccgtaggcgg tggccagcgc gctcgacgaa ccgaagtgca
     1441 gcaccgcggc cacgatcgcc gcgtactgca gccagttcac gcccgggatg tagatctggc
     1501 cggtgtcgcg caccgaggtg tgcaaaacgc gcaggcgcgg caggtagccg agctggatcg
     1561 cctgcttggt gaccgagaac gtgcccgaga tcgtcgcctg cgaggcgatc acggtggcga
     1621 gcgtcgccat cacgaccatc gggatcagga accagcccgg caccatgttg aagaacgggt
     1681 tgtcggcctt gcccggctcg accagcatca gcgcgccctg gccgaagtag ttcagtacca
     1741 atgccgggaa cacgagcacg aaccacgcga tgcggatcgg ccggctgccg aagtggccca
     1801 tgtcggcgta cagcgcctcg gcgccggtga cggccaggaa caccgcgccg agcaccagca
     1861 aggtgacgtg gccgtgttcg atgccgaagc tgatcgcgtg ccacgggttc agcgccccga
     1921 gcacctgcgg cgcgcgcacg atccagacca cgccccagaa gccgatcacc aggaaccaca
     1981 cgaccgtgaa cggcccgaac cagcgcccca tcgtgccggt gccgaaacgc tgcaccgcgt
     2041 acagcacggt gatcacggcc agcgtgatcg gcacgacgta gggtttgaag gccggcgtca
     2101 cgacctcgag gccctcgacc gccgagagca ccgagatcgc cggggtgatc acgccgtcgc
     2161 cgtagaacag cgccgcgccg aggatgccca tcacgatcag cgccgagcgc atccgcggcc
     2221 ggtctttggt ggcatgcgac gacagcgcca tcagcgccat cgtgccgccc tcgccgttgt
     2281 tgttggcgcg catgatcagc accacgtact tcagcgagac gatgatcgtc agcgtccaga
     2341 acacgagcga gagcacgccg aggatgtcgg gctgcgacag gcttgcgtgc ttggtggcga
     2401 agacttcgcg cagcgcgtac agcgggctgg tgccgatgtc gccgtagacg acgcccaggg
     2461 cgcccagggt cagggcggcc aggctggacg tgccggattt tctgctcaca gaaggtgacc
     2521 ccgctggtca gggcgtgggg cgtcggtcgg taggagaggg attctgcgcc cggccccccg
     2581 ggccctggca agccccggag ccggccatgt tgcggcgcag caacccggca cttgggagac
     2641 agcctcctag tcgtagacct cggcgtcagg atcggtcgct tcgagctcgt agctcgccgc
     2701 caccatcgcc agccgggcga tcacgccgta catgtagaag cggttcggcg cgctcgcgcc
     2761 gggcttcgca ccgggcttgg gcagctggtt gtgttcggcg aacgcgagcg gcgcgaagtg
     2821 ggcacccggc gcgttcaggt tctcgtcgat gccgcgctcg g
//
LOCUS       DASRDG010000065         6316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_10783, whole genome shotgun sequence.
ACCESSION   DASRDG010000065 DASRDG010000000
VERSION     DASRDG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10783"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1696
                     /locus_tag="VFA35_01040"
     CDS             74..1696
                     /locus_tag="VFA35_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZT54782.1"
                     /translation="MHAGPERHGRTRRLFTERTLPVQFALLGSAVAALLAGLAGWLVR
                     GPEGASIAAGAAGGALVVTLAATLLARRVARGLDRVEATARALRQDGDAATRHFALDP
                     ACAESQRATLALQRLVDAHRERVQALLASNLALGQRLSHRTHELSTLQDLSIGLATQS
                     GLHELVDEALGALEQTVEYASASVWARDADSAEARLLGYRSAAAGDARTEDLRGQRLS
                     RQNMEHYRQIERSREAIIVNHSRQNLLSWLWAKVVDDARSSSLYRATRAWMALPLRFR
                     DEVLGVLRVDHHEQGYFDAERAKLLKAIGSQAALAMHHAKLQEQERAVAVVAERNRIA
                     RELHDAVSQTLFAANVQAGTLAKLAGRPGALDAAALARQAQALERLTRGALAEMRLLM
                     YELRPDALRNTRLADLLQHAIGALQCRGEFEVAQRLAEREPLSDAVRTHVYRIAQEAI
                     SNAARHSGARRIAIEWTVDAPASATLRVVDDGRGFDPAQPRPGHFGLDNMQSRATEIG
                     AALSVTSTPGQGSEVRLVLPLAEAGAAPHPPP"
     gene            1693..2352
                     /locus_tag="VFA35_01045"
     CDS             1693..2352
                     /locus_tag="VFA35_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZT54783.1"
                     /translation="MTTTTRMSAPKIRVFVVDDHPLIRNGLAGMIDAEAGLEGVGEAG
                     SGDEAVQRLPALAPDVVLMDLVMPGLDGIEAVAALHPRLPQTRFLILTSLIDPAAVRR
                     AMAAGASGFLSKTASADELVAAIRSAHAGRRVLAPEATDALFEASRQAPTGGDLTPRE
                     RELLALMARGLSNQEIVAELAIAMPTVKFHIGNILGKLAVDNRTEAVLKALKHKLVPP
                     L"
     gene            2489..4513
                     /locus_tag="VFA35_01050"
     CDS             2489..4513
                     /locus_tag="VFA35_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZT54784.1"
                     /translation="MTNKLLLTAVAAAACAAIYARGETVTGANGARPANAVPGQCYAK
                     VLTPARFETRSEQVVRLPASQRIELVPATYKTVTEQVLVSPEYKRSISVPATYKTVTE
                     EVEAIPAGSRTEPIPGTYKTVTEQVQVVAASTRVEIVPATTKTVTERVLVTPERKEIR
                     TIPATYETVEESVVDRPASTRVETVPATYKVVSEEVLVKPESVRYEPIAIPFKKVTEE
                     VVRSEASKRLEAVAPKLKTVQERVLVKEASKRLVEVPAVFETVTERIKVADASTQWKR
                     GRAWLGQAKEVRPVKGFAFDAKGAPLADQPGQAAWVASDNTSLDDDVMCLVEIPERYE
                     TITKRVLKTPASVREVEVPAEYAMVSKQAVDQEASVREVEIPATYQTVTTQVIDVDTL
                     KAKGYKFADNGDIVATPTGERVLRASALNGGAAKAKSAGAESGEEGYVREIKIPAQYA
                     TVKRQVIDQPATVRTVEVPGTTKVVSHRAVKTPATTSEVLIPAVYKTVTREVVATTAS
                     TREIKIPAEFKTVSRQVVDTAPSTREIPVPAKLQTIAHRVVDQPAATREELVPAVYKT
                     VTRQVVDQPAATRAVEIPAQYETLTRSVKVADASTEWRSILCETNATPTKLREIQQAL
                     MKAGYNPGPIDGVIRAQTMRAVNDYQTAKGLPVDAYLNLETVKALGVAPN"
     gene            4546..5004
                     /locus_tag="VFA35_01055"
     CDS             4546..5004
                     /locus_tag="VFA35_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZT54785.1"
                     /translation="MPIRLFVASASLALLAFTATAQTQPASASASAAAPATAAAPAAK
                     AAPAADGGEGKVAYYGRKFHGRKTASGERFDAQALTMAHKTLPFGTLVRVTNLKNGRS
                     AVVRVNDRGPSTPDRIGDLSLAAARQLHMLRSGVIDARLETVGTAKRKKR"
     gene            5044..5472
                     /gene="mscL"
                     /locus_tag="VFA35_01060"
     CDS             5044..5472
                     /gene="mscL"
                     /locus_tag="VFA35_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZT54786.1"
                     /translation="MSFASDFKEFALKGNVVDLAVGVIIGAAFGKIVDSLVKDLIMPI
                     VGKIFGGLDFSNFFVMLSNPPADYKGAMTYDALTKAGVPLFAYGNFITVAINFLILAV
                     IIFIMVKQINRVKKDEPPAPAPATPEDVLLLRQIRDSLAK"
     gene            5558..5935
                     /locus_tag="VFA35_01065"
     CDS             5558..5935
                     /locus_tag="VFA35_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZT54787.1"
                     /translation="MIFGMNHFTVIAEDEHKTLAFYVDLLGLTVGPRPDLGFRGAWLY
                     AGGAQSVLHVVFDRPVPAQRAGVIDHMAFSARDLRGVKARFDASGTSYDLRRQAGAGT
                     WQLFCHDPSGARVELDFDASETL"
     gene            6032..>6316
                     /locus_tag="VFA35_01070"
     CDS             6032..>6316
                     /locus_tag="VFA35_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54788.1"
                     /translation="MSLDTISQTIDRALTAAGLNTRSGPMQTVTATIEKALAAAGIAQ
                     RRVAPQATAAPPAATGVGEFLALSHADGRTRRAYKLYVPSSYVGKPMPLIV"
BASE COUNT         1014 a         2292 c         2204 g          806 t
ORIGIN      
        1 aacgcgcggc cggcgccttc ggcaagcccg cgggcgacgc gggcggcgac cgctgagcag
       61 cgggcgcgcg ccgatgcacg ccggccccga gcgccatggc aggacgcggc gcctgttcac
      121 cgaacgcacg ctgccagtcc agttcgcgct gctcggctcg gccgtggccg cgctgctcgc
      181 cgggctggcc gggtggctgg tgcgtgggcc cgagggcgcg tcgatcgcgg cgggcgccgc
      241 cggcggcgcg ctggtcgtga cgctcgcggc gacgctgctg gcgcggcgcg tggcgcgcgg
      301 cctggaccgc gtcgaagcca cggcccgggc gctgcgccag gacggcgacg ccgccacccg
      361 ccacttcgcg ctcgacccgg cctgcgccga gtcgcagcgc gccacgctgg cgctgcagcg
      421 gctggtcgac gcccaccgcg agcgcgtgca ggcgctgctg gcgagcaacc tcgcgctcgg
      481 ccagcgcctg agtcaccgca cccacgagct gtcgaccttg caggatctgt cgatcgggct
      541 ggccacgcag tcgggcctgc acgagctcgt cgacgaggcg ctcggtgcgc tggagcagac
      601 cgtcgaatac gccagcgcct cggtctgggc gcgcgacgcc gacagcgccg aggcgcggct
      661 gctcggctac cgcagcgccg ccgcgggcga cgcgcgcacc gaggacctgc gcggccagcg
      721 cctgtcgcgc cagaacatgg agcactaccg gcagatcgag cgcagccgcg aagcgatcat
      781 cgtcaaccac tcgcggcaga acctgctgtc gtggttgtgg gccaaggtgg tggacgacgc
      841 gcgctcgtcg tcgctgtacc gcgccacgcg cgcctggatg gcgctgccgc tgcgctttcg
      901 cgacgaggtg ctgggcgtgc tgcgcgtcga tcaccacgag cagggctact tcgacgccga
      961 acgcgccaag ctgctcaagg cgatcggcag ccaggcggcg ctggcgatgc accacgccaa
     1021 gctgcaggag caggagcgtg ccgtcgccgt ggtcgccgag cgcaaccgca tcgcccgcga
     1081 gttgcacgac gcggtgtcgc agacgctgtt cgcggccaac gtccaggccg gcacgctggc
     1141 caagctcgct gggcggcccg gcgcgctgga cgccgctgcg ctcgcgcgcc aggcgcaggc
     1201 gttggagcgc ctgacccgcg gcgcgctcgc cgagatgcgg ctgctgatgt acgagctgcg
     1261 ccccgatgcg ctgcgcaaca cgcgcctggc cgacctgctc cagcacgcca tcggcgcgct
     1321 gcaatgccgc ggcgagttcg aggtcgcgca gcgcctggcc gaacgcgagc cgctgagcga
     1381 cgcggtgcgc acccacgtct accggatcgc gcaggaggcg atctccaatg cggcgcgcca
     1441 cagcggcgcg cgccgcatcg cgatcgagtg gaccgtcgat gcgcccgcca gcgcgacgct
     1501 gcgcgtcgtc gacgacggcc gcggcttcga cccggcacag ccgcggcccg gccacttcgg
     1561 cctcgacaac atgcaaagcc gcgcgaccga gatcggcgcc gcgttgagcg tcaccagcac
     1621 gcccggccag ggcagcgaag tgcggctggt tctcccgctg gccgaagccg gcgccgcgcc
     1681 ccacccaccg ccttgaccac caccacccgc atgtccgccc cgaagattcg cgtgttcgtc
     1741 gtcgacgacc accccctgat ccgcaacggc ctggccggca tgatcgacgc cgaggccggg
     1801 ctcgaaggcg tcggcgaggc cggcagcggc gacgaggcgg tgcagcggct gcccgcgctc
     1861 gcgcccgacg tggtgctgat ggacctggtg atgccgggcc tggacggcat cgaggccgtc
     1921 gccgcgctgc acccgcgcct gccgcagacg cgcttcctga tcctgaccag cctgatcgac
     1981 ccggccgcgg tgcgccgcgc gatggccgcg ggcgcgagcg gcttcctgtc gaagacggcg
     2041 tcggccgacg agctggtggc ggcgatccgc agcgcgcacg ccggccggcg cgtgctcgcg
     2101 cccgaggcga ccgacgcgct gttcgaggcg tcgcggcagg cgccgaccgg cggcgacctg
     2161 accccgcgcg agcgcgagct gctcgcgctg atggcgcgcg gcctgagcaa ccaggagatc
     2221 gtcgccgaac tcgcgatcgc gatgccgacc gtgaagttcc acatcggcaa catcctgggc
     2281 aagctggcgg tcgacaaccg caccgaggcc gtgctcaagg cgctcaagca caagctggtg
     2341 ccgccgctct gagcttcccc gactggggta tgggccgcgc cccgaccgcg acacggcgcg
     2401 ccggcgcgcc cgcatcaaca caatttcccg gtgccgtccg tgctgtacgg cagtgcgcgc
     2461 ttcgcagacc acccaaccga ggaaagccat gaccaacaaa ctgctcctga ccgccgtcgc
     2521 ggcggccgcc tgcgccgcca tctacgcccg cggggagacc gtgaccggcg caaacggcgc
     2581 acgccccgcc aacgccgtgc ccggccagtg ctacgccaag gtgctgacgc cggcgcgctt
     2641 cgagacgcgc tcggagcagg tggtgcggct gccggcgagc cagcgcatcg agctcgtgcc
     2701 cgcgacctac aagaccgtca ccgagcaggt gctggtcagc cccgagtaca agcgcagcat
     2761 cagcgtgccc gcgacctaca agacggtgac cgaggaggtc gaggcgatcc cggcgggctc
     2821 gcgcaccgag ccgatccccg gcacctacaa gacggtgacg gaacaggtgc aggtcgtcgc
     2881 cgccagcacc cgcgtcgaga tcgtgccggc caccaccaag accgtgaccg agcgcgttct
     2941 ggtgaccccg gaacgcaagg aaatccggac gatcccggcg acctacgaga ccgtcgagga
     3001 gagcgtcgtc gatcggccgg cgtcgacccg cgtcgagacc gtcccggcga cctacaaggt
     3061 cgtcagcgaa gaggtgctgg tcaagcctga gtcggtgcgc tacgagccga tcgcgatccc
     3121 gttcaagaag gtcaccgaag aagtcgtgcg cagcgaggcc agcaagcgcc tcgaggcggt
     3181 cgcgccgaag ctgaagaccg tgcaggagcg cgtgctcgtc aaggaagcca gcaagcgcct
     3241 ggtggaagtg ccggcggtgt tcgagaccgt gaccgagcgc atcaaggtgg ccgacgcgtc
     3301 gacgcagtgg aagcgcggcc gcgcctggct cggccaggcc aaggaggtgc ggccggtcaa
     3361 gggcttcgcg ttcgatgcca agggcgcgcc gctcgccgat caacccggcc aggcggcctg
     3421 ggtggcgagc gacaacacct cgctggacga cgacgtgatg tgcctggtcg agattccgga
     3481 gcgctacgag accatcacca agcgcgtgct gaagacgccg gcgagcgtgc gcgaggtcga
     3541 ggtgccggcc gaatacgcga tggtctccaa gcaggccgtc gatcaggagg cgtcggtgcg
     3601 ggaggtcgag attcccgcga cctaccagac cgtgacgacc caggtcatcg atgtcgacac
     3661 gctgaaggcc aagggctaca agttcgccga caacggcgac atcgtcgcca cccccaccgg
     3721 cgaacgcgtg ctgcgcgcct cggcgctgaa cggcggcgcg gccaaggcga agtcggccgg
     3781 cgccgagtcg ggcgaagaag gctatgtgcg cgagatcaag atcccggcgc agtacgcgac
     3841 cgtcaagcgg caggtcatcg accagccggc gacggtgcgc acggtcgagg tgccgggcac
     3901 gaccaaggtc gtcagccacc gcgccgtgaa gacgccggcc acgaccagcg aggtgctgat
     3961 cccggcggtc tacaagaccg tcacgcgcga ggtcgtcgcc acgacggcga gcacgcgcga
     4021 gatcaagatc ccggccgagt tcaagaccgt ctcgcgccag gtcgtcgaca ccgcgccgtc
     4081 gacgcgcgag atcccggtgc cggcgaagct gcagacgatc gcgcaccgcg tcgtcgacca
     4141 gccggcggcg acgcgcgagg agctggtgcc ggcggtctac aagaccgtga cgcgccaggt
     4201 cgtcgatcag ccggcggcga cgcgcgccgt cgagatcccg gcgcagtacg agacgctgac
     4261 gcgctcggtc aaggtcgccg acgcgagcac cgaatggcgc tcgatcctgt gcgagaccaa
     4321 cgcgacgccg accaagctgc gcgagatcca gcaggcgctg atgaaggccg gctacaaccc
     4381 cggcccgatc gacggcgtga tccgggcgca gacgatgcgc gcggtcaacg actaccagac
     4441 cgccaagggc ctgccggtcg acgcgtacct gaacctcgaa accgtcaagg cgctcggcgt
     4501 cgcgccgaac tgatccgctc ttcttgccac ctggaggact ttcacatgcc catccgactc
     4561 ttcgtcgcga gcgcctcgct cgccctgctc gccttcacgg caaccgccca aacgcagccg
     4621 gcgtcggcct cggcctcggc ggctgcaccg gccaccgccg cagcgccagc ggccaaggcc
     4681 gcacccgcgg ccgacggcgg cgaaggcaag gtcgcctact acggccgcaa gttccacggc
     4741 cgcaagaccg ccagcggcga gcgcttcgac gcgcaggcgc tgacgatggc gcacaagacg
     4801 ctgccgttcg gcacgctggt gcgcgtgacg aacctgaaga acggccgctc ggcggtggtg
     4861 cgcgtcaacg accgcgggcc gagcacgccg gaccgcatcg gcgacctgtc gctggccgcg
     4921 gcgcgccagc tgcacatgct gcgctccggg gtgatcgacg ccaggctcga aaccgtcggc
     4981 accgcgaagc ggaagaagcg ctgagcgcca ttccccaccc ccctcacctt ctctggagga
     5041 gccatgagct tcgcatccga tttcaaggaa ttcgccctga agggcaacgt cgtcgacctc
     5101 gcggtcggcg tgatcatcgg cgccgcgttc ggcaagatcg tcgactcgct cgtcaaggac
     5161 ctgatcatgc cgatcgtcgg caagatcttc ggcgggctgg atttctccaa cttcttcgtc
     5221 atgctgtcca acccgcccgc cgactacaag ggggcgatga cctacgacgc gctcaccaag
     5281 gccggcgtgc cgctgttcgc ctacggcaac ttcatcacgg tggcgatcaa cttcctgatc
     5341 ctcgcggtga tcatcttcat catggtcaag cagatcaacc gcgtgaagaa ggacgagccg
     5401 ccggcgcccg cgccggcgac gccggaagac gtgctgctgc tgcgccagat ccgcgactcg
     5461 ctcgcgaagt gagggcccgg atcgctcggg agtggcgtga gcgggggcgt ggcaggatcg
     5521 gtgcttcgat cctgcccgcc tgaaaggagc gcccgagatg atcttcggca tgaaccactt
     5581 caccgtgatc gcggaagacg agcacaagac gctcgcgttc tacgtcgacc tgctcggcct
     5641 caccgtcggc ccgcgacccg acctcggctt tcgcggcgca tggctctacg ccggcggcgc
     5701 gcagtcggtg ctgcacgtgg tgttcgatcg ccccgtgccc gcgcagcgcg ccggcgtgat
     5761 cgaccacatg gccttcagcg cgcgcgatct gcgtggcgtc aaggcgcgct tcgacgccag
     5821 cggcacgagc tacgacctac gccggcaggc gggcgccggc acttggcagc tgttctgcca
     5881 cgacccgagc ggcgcgcggg tcgagctcga tttcgatgcg tcggagacac tttgacgtag
     5941 ggcttgtccc acaaggtatc agggttcgca cgcggcgcgt tccgtccgca ggaggtgcat
     6001 gattgctgca gtgcagcatt caaggacatc cgtgagcctc gacaccattt cgcagaccat
     6061 cgaccgcgcg ctgaccgcgg ccggtttgaa cacccgctcg ggcccgatgc agaccgtcac
     6121 cgcgacaatc gagaaggcgc tggccgccgc gggcatcgcg cagcgccgcg tcgcgccgca
     6181 ggccaccgcg gctcccccgg ccgccaccgg tgtaggcgag tttctggcgc tgtcgcacgc
     6241 cgacggtcgc acccggcgcg cttacaagct gtacgtgccg tcgagctacg tcggcaagcc
     6301 gatgccgctg atcgtg
//
LOCUS       DASRDG010000066        10805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1516494, whole genome shotgun sequence.
ACCESSION   DASRDG010000066 DASRDG010000000
VERSION     DASRDG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516494"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /gene="leuC"
                     /locus_tag="VFA35_01075"
     CDS             <1..1377
                     /gene="leuC"
                     /locus_tag="VFA35_01075"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZT54789.1"
                     /translation="EHVVHTEDDGTAVLYIDRHLVHEVTSPQAFDGLDLAGRKVWRVS
                     ANLAVSDHNVPTTDRSHGITDPISKLQVDTLDANCDRVGITQFKMNDRRQGIVHVIGP
                     EQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLARKAKNMLVKVE
                     GRLGTGVSAKDLVLAIIGKIGTAGGTGYTIEFAGAGVRALSMEGRMTVCNMAIEAGAR
                     AGLIGVDETTIAYVKGRPYTPSGLEWEQALRYWRTLSSDPGAHFDAVVELDASTIRPQ
                     VTWGTSPEMVLSIDDRVPDPDREKDAGKRGAIERALQYMALEPNKRIADVSIDKVFIG
                     SCTNSRIEDLREAANVVRHLGKRIAKNVRLAMVVPGSGLVKEQAEREGLDVIFRNAGF
                     EWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGH
                     FVDVSRLA"
     gene            1399..2043
                     /gene="leuD"
                     /locus_tag="VFA35_01080"
     CDS             1399..2043
                     /gene="leuD"
                     /locus_tag="VFA35_01080"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZT54790.1"
                     /translation="MTPFRIHTGLVAPMDRENVDTDAIIPKQFLKSIKRSGFGPNLFD
                     EWRYLDHGEPGQDPTTRRPNPDFVLNQPRYQGASVLLARKNFGCGSSREHAPWALEQF
                     GFRALIAPSYADIFFNNCFKNGLLPIVLPEEQVARLFDEAAAFIGYTLTIDLARQVVV
                     KPDGTTVPFEVEPFRKYCLLGGFDDIGLTLRHADKIRAFEAERLLTKPWLAHTI"
     gene            2070..3173
                     /gene="leuB"
                     /locus_tag="VFA35_01085"
     CDS             2070..3173
                     /gene="leuB"
                     /locus_tag="VFA35_01085"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZT54791.1"
                     /translation="MKIAILPGDGIGTEIVAQAVRVLGVLDLALETETALVGGAAYDA
                     HGHPLPEATLKLAQGADAVLFGAVGDWRYDKLERALRPEQAILGLRKSLGLFANFRPA
                     ICYEQLTHASSLKPELVAGLDILIIRELTGDIYFGQPRGRRTSIDGAFKGAEEAFDTM
                     RYARPEIERIAHVAFQAARKRGKRVTSVDKANVLETFQFWKDIVTEVHAQYPDVALDH
                     MYVDNAAMQLVKDPKKFDVIVTGNMFGDILSDEAAMLTGSIGMLPSASLNAAGRGLYE
                     PSHGSAPDIAGKNIANPLATILSAAMMLRFSLDQPAAADRIESAVQQVLAKGLRTADI
                     WSEGTTKVGTIEMGDAVVAALASSTAKTTMTKG"
     gene            3262..4392
                     /gene="asd"
                     /locus_tag="VFA35_01090"
     CDS             3262..4392
                     /gene="asd"
                     /locus_tag="VFA35_01090"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZT54792.1"
                     /translation="MTALTGLVGWRGMVGSVLMDRMQAEGDFDHIEPMFFSTSNAGGA
                     APAMAKNETTLFDAHDIDALKRCEIIVTAQGGDYTSDVFPRLRAAGWKGHWIDAASTL
                     RMKDEAVIVLDPVNMPVIKDALARGGRNWIGGNCTVSCMLMGVGALYKAGLVEWMSTM
                     TYQAASGGGAAHMRELLTQYGTLNASVRHLLDDPKSAILEIDRQLAATQRGLSEAEFA
                     NFGVPLGGSLIPWIDKDLGDGMSKEEWKGEAETNKILAQGAGFGSAPIPVDGFCVRVG
                     AMRCHSQALLFKLKKGVPLADIEALIANDNAWVKLVPNTKEATLRDLTPVAVTGTMTI
                     PVGRLRKMSMGPEYLGAFTIGDQLLWGAAEPLRRMLRIVIEA"
     gene            4634..7213
                     /locus_tag="VFA35_01095"
     CDS             4634..7213
                     /locus_tag="VFA35_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HZT54793.1"
                     /translation="MKRNLTFSGRFALTGVAAAALMLVAPAAWSLGLGRIQVQSALGE
                     SLRAEIDVTSITPEEAANLRVRVAPPDSYKAAGVDYNAVLPSTQVELLRRADGRPYLK
                     LTSDRGVQEPFVDVILEINWATGRLVREYTMLFDPPVTARAPAPAATTAPTMSAAAPV
                     PPRTAAASRPLPAPAVTRAAPAEPPAPREPRSAPKPAVAAAPTGADEYKVRPGDSLSK
                     IASRTQRQGVSLDQMLVSLYRGNPQAFVDNNMNRLKAGVVLAVPSAETAGGVTASDAR
                     QVIQAQSADFGAYRQRLAGAVPTAKAEGSSRQASGKVQASVDDRKAGAATAPDKLTLS
                     KGAAGAKAEEQISKDHAKKADDVRVAELSKNVDELKRLSGAASAATAKPVAVPASAPA
                     SAPAVAAKPAPAAPSMPAPTVVAAAPAPVAASAAAAPPPAASVAAMPTASAPVVAVAP
                     SAPVVAPPVKPAASAAPAAKPPVKAPVEEPSVIGSLLDDNPMALGLGGLALVLLAGFG
                     IYKFGKRARKDSGETSFLESRLQPDSFFGASGGQRIDTRDAAGTSSSMSYSLSQLDAI
                     GDVDPVAEADVYLAYGRDLQAEEILKEAMRSSPDRMAIRTKLLEVYAKRRDTKGFEML
                     ATQLFALTRGEGEDWAKAQELGAQIDPENPLFKPGGAPAATRHGAGGEIIEPLGASTM
                     PQSVLPSPSQFGNSVSGALIADSGNTGVDLDLDLDAPEAGTPTAPSPLQATPVVEPAP
                     YRDAADSRSLDFSVPDVPPLTSKPAHAAEPSAFDLSDISLDLDMPIAPPAAAPASSGF
                     GDAGLPPLDGDDDPLARKLELAEEFRQIGDTDGARDLLHEVIAKSSGAVKSRAQSMLA
                     DLG"
     gene            7239..8024
                     /gene="truA"
                     /locus_tag="VFA35_01100"
     CDS             7239..8024
                     /gene="truA"
                     /locus_tag="VFA35_01100"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZT54794.1"
                     /translation="MRVVLGVAYRGQAYRGWQSQPDGNTVQDRFERALARFADAPTRT
                     VCAGRTDTGVHALNQVVHLDTEIVRAESSWVRGTNRFLPPDIAVQWCLLKDDDSFHAR
                     YAALGRRYVYVLLESAVRPAVEAGSVGWCFRPLDADAMRSAAAQLLGEHDFSAFRSSE
                     CQAPTPVKTIRSIAISRRGAYWRFEFDANAFLHHMVRNLMGALLAVGAGGRPQTWLAD
                     VLASKDRALAAPTFPADGLYFVGPYYDPIHAIPRHTAAMDWLP"
     gene            8021..8683
                     /locus_tag="VFA35_01105"
     CDS             8021..8683
                     /locus_tag="VFA35_01105"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZT54795.1"
                     /translation="MICPPMNQRTRIKICGLTREADVDAAVDAGADAVGFVFYDKSPR
                     HVSLARARELAARLPAFVMPVGLFVNAGEAEIAAAVAAIPQLLLQFHGDESPQDCARA
                     GRPFLRAARVAPGFDLLDFASRFAHAQAILLDAHVEGYGGGGKVFDWSLIPSAVHLPV
                     VLSGGLHAGNVIQGIQLLRPWAVDVSSGVESAKGIKDADAIRRFCDAVREADARRAAS
                     PA"
     gene            8700..9947
                     /gene="trpB"
                     /locus_tag="VFA35_01110"
     CDS             8700..9947
                     /gene="trpB"
                     /locus_tag="VFA35_01110"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZT54796.1"
                     /translation="MSNYQQPDASGHFGPYGGSFVAETLVHALDELKRAYAEVQNDPA
                     FIKEFQYELKHFVGRPSPIYHAARLSRELGGAQIFLKREDLNHTGAHKVNNTIGQAML
                     ARRMGKPRVIAETGAGQHGVATATICARYGLECVVYMGSEDVKRQSPNVYRMNLLGAT
                     VVPVESGSKTLKDALNEALRDWVTNVENTFYIIGTVAGPHPYPTMVRDFQRVIGDECL
                     VQMPEMTGRQPDAVIACVGGGSNAMGIFYPYIDHAGTRLIGVEAAGQGLETGKHAASL
                     SAGSPGVLHGNRTYLLQDANGQIIETHSISAGLDYPGVGPELAYLKDIGRAEYVGITD
                     REALEAFHRLCRTEGIIPALESSHAVAYAMKLAPTMRPDQNLLVNLSGRGDKDIGTVA
                     DLSGAAYFDRPSQGGQSVKGLAA"
     gene            9944..10741
                     /gene="trpA"
                     /locus_tag="VFA35_01115"
     CDS             9944..10741
                     /gene="trpA"
                     /locus_tag="VFA35_01115"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZT54797.1"
                     /translation="MSRIKTTFDALQASGRKALIPYVTAGDPFADATVEIMLAMAHAG
                     ADVIELGVPFSDPMADGPVIQKAGERALARGIGMPQVLDFVRGFRAENDATPVVLMGY
                     ANPVERYGVDRFVADARAAGVDGVLVVDYPPEECETFAATLRASGLDPIFLLAPTSTE
                     ARMAHVGRIASGYVYYVSLKGVTGAGHLDTAAVAAMVPRIKTHVKLPVGVGFGIRDAK
                     TAQAVAAVSDAVVIGSALVQLLETRTRENVAAAAGEFIAGIRAALDS"
BASE COUNT         1764 a         3712 c         3702 g         1627 t
ORIGIN      
        1 gagcacgtcg tccacaccga agacgacggc acggccgtgc tctacatcga ccgccacctc
       61 gtccatgagg tcaccagccc gcaggcgttc gacggcctcg atctcgcggg ccgcaaggtc
      121 tggcgggttt cggcgaacct ggcggtcagc gaccacaacg tgccgaccac cgaccgctcg
      181 cacggcatca ccgacccgat ctcgaagctg caggtcgaca cgctcgacgc caactgcgac
      241 cgcgtcggca tcacacagtt caagatgaac gatcgccgcc agggcatcgt tcacgtgatc
      301 gggccggagc agggtgccac gctgccgggc atgactgtcg tctgtggcga ctcgcacacg
      361 tcgacgcacg gcgcgttcgg cgcgctcgcg cacggcatcg gcaccagcga ggtcgagcac
      421 gtgctcgcga cgcagacgct gctcgcgcgc aaggcgaaga acatgctcgt gaaggtcgaa
      481 ggtcggctcg gcaccggggt cagcgcgaag gacctggtgc ttgcgatcat cggcaagatc
      541 ggcactgccg gcggcaccgg ctacacgatc gagttcgccg gcgcgggcgt ccgcgcgctg
      601 agcatggaag gccggatgac cgtgtgcaac atggcgatcg aggccggcgc gcgcgccggc
      661 ctgatcggcg tcgacgagac gacgatcgcc tacgtgaagg gtcgcccgta cacgccgagc
      721 ggcctcgagt gggagcaggc gctgcgctac tggcgcacgc tgagctccga cccgggcgcg
      781 cacttcgacg cggtcgtcga actcgacgcg tcgacgatcc gcccgcaggt cacctggggc
      841 acatcgcccg agatggtgct gtcgatcgac gaccgcgtgc ctgatcccga ccgcgaaaag
      901 gacgccggca agcgcggcgc catcgagcgc gcgctgcagt acatggcgct cgagccgaac
      961 aagcgcatcg ccgacgtctc gatcgacaag gtcttcatcg gctcgtgcac gaactcgcgc
     1021 atcgaagact tgcgcgaggc agcgaacgtg gtacgccacc tgggcaagcg catcgcgaag
     1081 aacgtgaggc tcgcgatggt cgtgccgggt tcggggctgg tgaaggaaca ggccgagcgc
     1141 gaggggctgg acgtgatctt caggaacgcc ggcttcgagt ggcgcgagcc cggctgctca
     1201 atgtgcctgg cgatgaacgc cgaccggctc gagccgggcg agcgctgtgc gtcgaccagc
     1261 aaccgcaact tcgagggccg ccagggcgcc ggtgggcgca cccacctcgt gtcgccggcg
     1321 atggccgcgg cggccgcgat cgaaggccat ttcgtcgacg tgagccgact cgcgtgagcc
     1381 gacattccga gtgacaccat gactccgttt cgcatccaca ccggcctggt cgctccgatg
     1441 gaccgggaga acgtcgacac cgacgcgatc attccgaagc agttcctgaa gtcgatcaag
     1501 cgctccggct tcggcccgaa cctgttcgac gaatggcgct acctcgatca cggcgagccg
     1561 ggacaggacc cgaccacgcg ccggccgaac cccgacttcg tgctgaacca gccgcgctac
     1621 cagggcgcct ctgtgctgtt ggcgcgcaag aacttcggct gcggctcgtc gcgcgagcac
     1681 gcgccgtggg cgctcgagca gttcggcttt cgcgcgctga ttgcgccgag ctatgccgac
     1741 atcttcttca acaactgctt caagaacggc ctgctgccga tcgtgctgcc ggaagagcag
     1801 gtcgcgcgcc tgttcgacga ggccgcggcc ttcatcggct acacgctgac gatcgacctg
     1861 gcgcgccagg ttgtcgtcaa gcccgacggc acgacggtcc cgttcgaggt cgagcccttc
     1921 cgcaagtact gcctgctcgg cggcttcgac gacatcggcc tgaccttgcg ccacgccgac
     1981 aagatccgcg cgttcgaggc cgagcggctg ctgaccaagc cctggctcgc gcacacgatc
     2041 tgacccgaac gacaagaagg acgctccccg tgaagattgc aatcctgccg ggcgacggca
     2101 tcggcaccga gatcgttgcg caggccgtgc gcgtgctggg cgtgctcgac ttggcgctag
     2161 agaccgagac cgcactggtc ggcggcgcgg cctacgacgc gcacggccac ccgctgcccg
     2221 aagcgacgct gaaactcgcg caaggcgccg acgcggtgct gttcggcgca gtcggcgatt
     2281 ggcggtacga caagctcgag cgcgcgttgc gccccgagca ggcgatcctc gggctgcgca
     2341 agagcctggg cctgttcgcg aacttccgtc cggcgatctg ctacgaacag ctcacccatg
     2401 cgtcgagcct gaagcccgag ctggtcgcgg ggctggacat cctgatcatc cgcgagctca
     2461 ccggcgacat ctacttcggc cagccgcgcg gccggcgcac ctcgatcgac ggcgcgttca
     2521 agggcgccga ggaagccttc gacacgatgc gctacgcgcg ccccgagatc gagcgcatcg
     2581 cgcatgtcgc gttccaggcg gcgcgcaagc gcggcaagcg ggtgaccagc gtcgacaagg
     2641 ccaacgtgct cgagaccttc cagttctgga aggacatcgt caccgaggtc catgcgcagt
     2701 accccgatgt cgcgctcgac cacatgtacg tcgacaacgc ggcgatgcag ctggtgaagg
     2761 acccgaagaa attcgacgtg atcgtcaccg gcaacatgtt cggcgacatc ctctccgacg
     2821 aggccgcgat gctgaccggc tcgatcggca tgctgccttc ggcgtcgctg aacgcggccg
     2881 gccgcggtct gtatgaaccg agccacggca gcgcgcccga catcgccggc aagaacatcg
     2941 cgaatccgct ggccactata ctgtcggccg ccatgatgct gcgcttctca ctcgaccagc
     3001 ccgccgccgc cgaccgcatc gagtccgcgg tgcagcaggt gctggccaag ggtttgcgca
     3061 cggcagacat ctggtccgaa ggcacgacca aggtcggcac gatcgagatg ggcgacgccg
     3121 tcgttgcggc gctcgcgtct tcgaccgcga aaaccacgat gaccaagggc tagcaagctc
     3181 cggttcgtgt cgccccatcc cttggcgagc gagccggggg agcaaagagg tttcgatcga
     3241 ctttttcact ggggcacttt gatgacagca ctcacgggtt tggttggatg gcgcggaatg
     3301 gtcggttccg tgctgatgga tcgcatgcag gccgaaggcg acttcgacca catcgagccg
     3361 atgttcttca gcaccagcaa cgccggcggc gccgcgccgg cgatggcgaa gaacgagacg
     3421 acgctgttcg acgcccacga catcgacgcg ctgaagcgtt gcgagatcat cgtcaccgcg
     3481 caaggcggcg actacaccag cgacgtgttc ccgcgcctgc gcgccgccgg ctggaagggc
     3541 cactggatcg acgccgcctc gacgctgcgc atgaaggacg aggctgtcat cgtgctcgac
     3601 ccggtcaaca tgcccgtgat caaggacgcg ctggcccggg gcggccgcaa ctggatcggc
     3661 ggcaactgca ccgtgagttg catgctgatg ggcgtgggcg cgctctacaa ggccgggctg
     3721 gtcgagtgga tgagcacgat gacctaccag gcagcgtcgg gcggcggcgc cgcgcacatg
     3781 cgcgagctgc tgacgcaata cggcacgctg aacgcgtcgg tgcggcatct gctcgacgac
     3841 ccgaagagcg cgatcctcga gatcgatcgc cagctcgcgg ccacgcagcg cggcctgagc
     3901 gaagccgagt tcgccaactt cggcgtgccg ctcggcggca gcctgatccc gtggatcgac
     3961 aaggacctcg gcgacggcat gagcaaggaa gagtggaaag gtgaggccga gaccaacaag
     4021 atcctcgccc agggcgcggg cttcggcagc gccccgatac cggtcgatgg cttctgcgtg
     4081 cgcgtcggcg cgatgcgctg ccacagccag gcgctgctgt tcaagctgaa gaagggcgtc
     4141 ccgctcgccg acatcgaggc gctgatcgcc aacgacaacg cctgggtcaa gctcgtgccc
     4201 aacaccaagg aagccaccct gcgcgacctg acgccggtgg ccgtgaccgg cacgatgacg
     4261 atcccggtcg ggcgcctgcg caagatgtcg atggggccgg agtacctggg cgccttcacg
     4321 atcggcgatc agttgctgtg gggtgccgcc gagccgttgc ggcgcatgtt gcgcatcgtg
     4381 atcgaggcct gatcggttca ctgtggcgcg acggtgttgc tgcgctgcga cacgcgccgc
     4441 cgggcggcgc ttgcccgtga actgctcgca atgtcagcca ttgcattagc ttgtcagcgt
     4501 atctcattga tgctattgtg atttcgacct tctggccgga gatatgcgtg aactgcggcg
     4561 gaaatcgcga caatggtgct gtcgacattg gcgggtgcgt tcgagcacct gcctggtctt
     4621 attgggagac gccttgaaaa gaaacttgac gttctcgggg cgtttcgccc tgactggggt
     4681 ggccgccgct gcgttgatgc tggtcgcgcc ggccgcttgg tcgctcggcc tcgggcggat
     4741 tcaggtgcag tcggcgctgg gtgagtcgct tcgtgccgag atcgatgtca ccagcatcac
     4801 gccggaagaa gccgcgaacc tgcgcgtccg cgtcgcgccg ccggattcgt acaaggccgc
     4861 cggtgtcgac tacaacgccg tgctgcccag cacccaggtc gagttgctgc gccgcgccga
     4921 tggccgtccc tacctgaagc tcaccagcga ccgcggcgtg caggagccct tcgtcgacgt
     4981 gattctcgag atcaactggg cgaccgggcg gctggtgcgc gagtacacga tgctgttcga
     5041 cccgccggtc accgcgcgtg ccccggcgcc ggccgccacc accgcgccga cgatgtcggc
     5101 agccgcgccg gtgccgccac gcaccgcggc cgcgagccgg ccgctgcccg cgcctgcggt
     5161 gacccgtgcc gcgccagccg agccgccggc gccgcgcgag ccgcgctcgg cgccgaagcc
     5221 ggccgtcgcc gccgcaccga ccggcgccga cgagtacaag gtcaggccgg gcgattcgct
     5281 gtcgaagatc gcttcacgca cgcagcgcca gggcgtgtcg ctcgatcaga tgctggtgtc
     5341 gctgtaccgc ggcaacccgc aggccttcgt cgacaacaac atgaaccggt tgaaggccgg
     5401 cgtcgtgctc gcggtgccga gcgccgagac ggcgggcggc gtgaccgcat cggatgcgcg
     5461 ccaggtgatc caggcgcaga gcgccgactt cggcgcctat cgccagcgcc tcgccggcgc
     5521 ggtgccgacc gcgaaggccg aaggctcgtc gcgccaggcc agcggcaagg tgcaggcttc
     5581 ggtcgacgac cgcaaggcgg gcgcggctac cgcgcccgac aagctgaccc tgagcaaggg
     5641 cgccgccggt gccaaggccg aggagcagat ttccaaggac cacgccaaga aggccgacga
     5701 cgtgcgcgtc gccgagctct cgaagaacgt cgacgagctg aagcggcttt cgggtgccgc
     5761 cagcgcggct accgcgaagc cggttgccgt gcctgcatcg gcgcccgcgt ccgcaccggc
     5821 cgtggcggcg aaacctgcgc cggccgctcc gtcgatgccc gcgccgacgg tggtggcggc
     5881 tgcgccggca ccggttgccg cttccgccgc ggcagcgccg ccgccggcgg cgtccgtcgc
     5941 ggccatgccg acggcatcgg cgcccgtggt cgcggtggcg ccgtccgccc cggtggtggc
     6001 gccgccggtc aagccggccg cgagtgcggc accggcggcc aaaccgccgg tcaaggcgcc
     6061 ggtcgaagag ccgagcgtga tcggctcgct gctcgacgac aacccgatgg cgctgggcct
     6121 gggtgggctc gcgctcgtgt tgctggccgg gttcggcatc tacaagttcg gcaagcgcgc
     6181 gcgcaaggac agcggcgaaa cctcgttcct cgaaagtcgc ctgcagccgg actcgttctt
     6241 cggcgccagc ggcggccagc gcatcgacac gcgcgacgcc gcaggcacct cgtcgtcgat
     6301 gagctattcg ctgagtcagc tcgatgcgat cggtgatgtc gacccggtcg ccgaggccga
     6361 tgtctacctg gcctacgggc gcgacctgca ggccgaggag atcctcaagg aggcgatgcg
     6421 cagcagtccg gatcgcatgg cgatccggac caagctgctc gaggtctacg ccaagcgccg
     6481 cgacaccaag ggcttcgaga tgctcgcgac ccaattgttc gcgctgaccc gcggcgaggg
     6541 cgaggactgg gccaaggccc aggaactggg cgcgcagatc gacccggaga acccgctgtt
     6601 caagccgggc ggcgcgccgg cggcgacgcg gcatggcgcc ggcggcgaga tcatcgagcc
     6661 gctgggggcc agcacgatgc cgcaatcggt gctgccgtcg ccatcgcagt tcggcaactc
     6721 ggtgagcggc gcgctgatcg ccgacagcgg caacacgggc gtcgacctcg acctggatct
     6781 cgacgccccg gaggcgggca cgccgaccgc gccgtcgccg ctgcaggcca cgccggtggt
     6841 cgagccggcg ccgtatcgcg atgccgctga ctcgaggtcg ctcgacttct cggtgcccga
     6901 cgtgccgccg ctgacctcga agccggcaca tgccgccgaa ccgtcggcgt tcgatctgtc
     6961 cgacatctcg ctcgatctcg acatgccgat cgcgccgccg gccgcagcgc ctgcatcgtc
     7021 cggcttcggc gatgccggac tgccgccgct cgatggtgac gacgacccgc tggcgcgcaa
     7081 gctcgaactg gctgaagagt tccgccagat cggcgacacc gatggtgcgc gcgacctgct
     7141 gcacgaggtc atcgccaagt cgagcggtgc cgtgaagagc cgcgcgcaaa gcatgctggc
     7201 cgacctcggg tgaggtcgct gacgaccggg gcccgctcgt gagggtggtg ctcggcgtcg
     7261 cgtaccgtgg gcaggcctat cggggttggc agagtcaacc cgatggcaac acggtccagg
     7321 accggttcga acgcgcgctc gcacgtttcg ccgatgcacc gacgcgcacc gtgtgcgccg
     7381 gccgcaccga caccggcgtg cacgcgctga accaggtcgt gcatctcgac accgagattg
     7441 ttcgcgccga atcgtcatgg gtgcgcggca cgaaccggtt cctgccaccc gacatcgcgg
     7501 tgcagtggtg cctgctcaag gacgacgaca gcttccacgc gcgctacgcg gcgctcggcc
     7561 ggcgctacgt gtacgtgctg ctcgaatcgg cggtgcggcc tgcggtcgag gctggttcgg
     7621 tcggctggtg ctttcggccg ctcgacgccg atgcgatgcg ctcggccgcg gcgcaactgc
     7681 tcggcgagca cgacttcagc gcgttccgct cgtccgagtg ccaggcgccg acaccggtca
     7741 agacgatccg gtcgatcgcg atctcgcggc gcggcgcgta ctggcgcttc gagttcgacg
     7801 ccaatgcgtt tctgcaccac atggtccgca acctgatggg ggcgttgctg gcggtcggcg
     7861 cgggcgggcg cccccagacg tggctggccg acgtgctcgc ctcgaaggat cgcgcgctgg
     7921 cggcgccgac attcccggcc gacggtctgt acttcgtcgg gccgtactac gatccgatcc
     7981 acgccattcc gcgacacacc gccgcgatgg actggttgcc atgatctgcc caccgatgaa
     8041 ccaacgcacc cgcatcaaga tctgcggcct cacgcgcgag gccgatgtcg acgcggccgt
     8101 cgatgctggc gccgacgcgg tcggcttcgt gttctacgac aagagcccgc gccacgtgag
     8161 tctggcgcgt gcgcgcgagc tcgctgcgcg cttgccggcg ttcgtgatgc cggtcggcct
     8221 gttcgtcaac gccggcgagg ccgagatcgc cgccgcggtg gcggcgatcc cgcagttgct
     8281 gctgcagttc cacggcgacg agagccctca ggactgcgcc cgcgccggac gccccttcct
     8341 gcgtgccgcc cgcgtggcgc cgggcttcga tttgttagac ttcgcatccc gattcgcgca
     8401 tgcccaagcc atcctgctcg acgctcacgt cgagggatat ggcggcggtg gaaaggtttt
     8461 cgattggtcg ctcatcccct ccgccgtgca ccttccagtc gttttgtctg gtgggttgca
     8521 tgccggaaat gtgatccaag gcatccagct gctgcggccc tgggccgttg acgtcagctc
     8581 cggcgtggag tccgcgaaag gcatcaagga tgccgacgcg atccgccggt tctgcgatgc
     8641 agtgcgtgaa gcggatgccc gccgcgctgc atcgcccgcc tgatcaatcc acgttcacca
     8701 tgtccaacta tcaacaaccc gatgcctcgg gccatttcgg cccctatggc ggcagcttcg
     8761 tcgccgagac gctggtgcac gcgctcgacg agctgaagcg cgcgtatgcc gaggtccaga
     8821 acgacccggc gttcatcaag gaattccagt acgaactgaa gcactttgtc ggccgaccca
     8881 gcccgatcta ccacgcggcg cggctgagcc gcgagctcgg cggcgcgcag atctttctca
     8941 agcgcgaaga cctgaaccac accggcgcgc acaaggtcaa caacacgatc ggccaggcga
     9001 tgctcgcgcg ccgcatgggc aagccgcgcg tgatcgccga gaccggcgcc ggccagcacg
     9061 gcgtggccac cgcaacgatc tgcgcgcgct acgggctgga gtgcgtcgtc tacatgggca
     9121 gcgaggacgt gaagcgccag tcgccgaacg tctaccgcat gaacctgctg ggcgcgaccg
     9181 tggtgcccgt cgaaagcggc tcgaagacgc tgaaggacgc gctgaacgag gcgctgcgcg
     9241 actgggtcac gaacgtcgag aacaccttct acatcatcgg caccgtcgcc ggcccgcacc
     9301 cgtatccgac gatggtgcgc gacttccagc gcgtgatcgg cgacgagtgc ctggtgcaga
     9361 tgcccgagat gaccggccgc cagcccgatg cggtgatcgc ctgcgtcggc ggcggcagca
     9421 atgcgatggg catcttctac ccgtacatcg accatgccgg cacgcgcctg atcggcgtcg
     9481 aggcggccgg gcagggcctg gagaccggca aacatgccgc gtcgctgagc gccggctcgc
     9541 cgggcgtgct gcacggcaac cgcacctacc tgctgcagga cgcgaacggc cagatcatcg
     9601 agacgcactc gatctcggcc gggctcgact accccggcgt cggccccgag cttgcgtacc
     9661 tgaaggacat cggtcgcgcc gagtacgtag gcatcaccga ccgcgaggcg ctggaagcct
     9721 ttcaccgcct gtgccgcacc gagggcatca tcccggcgct cgaatcgagc cacgcggtgg
     9781 cctacgcgat gaagctggcg ccgacgatgc gcccggatca gaacctgctc gtgaacctgt
     9841 cgggtcgcgg cgacaaggac atcggcactg ttgccgacct ctccggcgcc gcgtacttcg
     9901 accggccctc gcagggcggc cagagcgtga aggggctggc agcatgagcc gcatcaagac
     9961 caccttcgac gcgttgcaag ccagcggccg caaggcgctg atcccctacg tgaccgcggg
    10021 cgacccgttc gctgacgcga ccgtcgagat catgctggcg atggcgcatg ccggcgccga
    10081 cgtgatcgaa ctcggcgtgc cgttctccga cccgatggcg gacggcccgg tgattcagaa
    10141 ggccggcgag cgtgcgctgg cgcgcggcat cgggatgccg caggtgctcg acttcgtgcg
    10201 cggctttcgc gccgagaacg acgcgacgcc ggtcgtgctg atgggttacg caaacccggt
    10261 cgaacggtac ggcgtcgatc ggttcgtcgc ggatgcccgc gccgccggcg tcgatggcgt
    10321 gctcgtggtc gactacccgc ccgaagaatg cgaaaccttc gccgcgacct tgcgtgcgag
    10381 tggcctcgat ccgatcttcc tgctcgcgcc gacctcgacc gaagcgcgca tggcgcatgt
    10441 cgggcgcatc gcgagcggct acgtctacta cgtctcgctg aaaggcgtga ccggtgccgg
    10501 ccacctcgac accgcggcgg tcgcggcgat ggtgccgcgc atcaagacac acgtgaagct
    10561 gccggtcggc gtgggcttcg gcatccgcga cgcgaagacg gcgcaggccg tcgcggcggt
    10621 gtcggacgcg gttgtgatcg gcagcgcgtt ggtacagctg ctggagacgc gcacgcgcga
    10681 gaatgtcgca gcggccgccg gcgagttcat cgctggtatc cgcgccgcgc tggattcctg
    10741 aaaggaacaa catgagctgg ttggaaaaac tgctgccccc gaagattcag ccgaacgagc
    10801 gcggc
//
LOCUS       DASRDG010000067         4863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_246607, whole genome shotgun sequence.
ACCESSION   DASRDG010000067 DASRDG010000000
VERSION     DASRDG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246607"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VFA35_01120"
     CDS             <1..1140
                     /locus_tag="VFA35_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZT54798.1"
                     /translation="SNGPGRRGFMLSAGSLVTTPLLAGIPRSASAQPAAASRPSTSTL
                     GRRTLGALEVSSLGLGVQNMSRTYQTTVPSRPEMINIIRTAFDRGVTFFDAAEAYGPH
                     EVERILGEGVAPIRDQVVIASKFGWDIDLKTGERRAGLNSRPDHIRLAVDGMLTRLRT
                     DRIDLLYQHRVDPAVPIEDVAGAVKDLMAQGKVLHWGLCEMGPNTLRRAHAEQRVTAV
                     QNEYSMLWRGPEKWLLPLCEELGIGFVPWSPLGVGFLTGAIDAKTRFADGDIRKGESR
                     LSPENLPHNLALVELVKRWAQRKGATPAQTALAWLTAQKPWIVPIPGTTQMPHMLDNI
                     GAAAIRFTSAELVELNAAVSMIQIRGARLPDAVLAFSDVEAPAKR"
     gene            complement(1337..1558)
                     /locus_tag="VFA35_01125"
     CDS             complement(1337..1558)
                     /locus_tag="VFA35_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HZT54799.1"
                     /translation="MGIEWMKPGVIVGSIVFALIGVAIFWICFVIVDKLTPYDLWGEI
                     VEKKNVALAIVVGAMCIAIGLIVAAAVHG"
     gene            complement(1599..3194)
                     /locus_tag="VFA35_01130"
     CDS             complement(1599..3194)
                     /locus_tag="VFA35_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HZT54800.1"
                     /translation="MATEPSPQRAYRAACPNCGAPVEFRSAASAFAVCSFCKSTVVRE
                     GDALRRIGQSAELFDDHSPLQLGAAGRYQGAAFTLVGRLQYRYAEGTWNEWHALFGNG
                     KSGWLSEDNGRYVIAFDAPLDGAAPSATSLKAGAPVVIDRQPWTVASVVAASVIAAQG
                     ELPTPPVTDRGFTVCDLRNTRGEVGTLDYGEAGAPRWSIGRAVAISELAMTGLAEPAE
                     KTLKGRAIECPSCGASLDVKLATTQSIVCPQCHAVVDVSHGIGGELQHYAQANGTPPL
                     IPLGSTGTLALGKAALPWQVVGYVERCEAPASDDDERTFWREYLLYHRTEGFAFIVDA
                     EDGWSWTAPLTGVPERAGSGVKYENVVYRKLYDYTGMVTYVLGEFYWQVTQNQRTVNT
                     DYQGTGSAAAKRLNREQTAAADTEEVVWSGGETLSADAVMKAFRLAPDKGAALARDAL
                     PTSSNPASLLAKIFFWVFVVVVVLLLFRCGSSSSSNCDATRDTFGAASSEYQNCLNSN
                     RSGGSYGSSGGSFGGFSSGGGHK"
     gene            complement(3215..4252)
                     /locus_tag="VFA35_01135"
     CDS             complement(3215..4252)
                     /locus_tag="VFA35_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZT54801.1"
                     /translation="MALMDFIKKQFIDIIEWTESGDGTLAWRYPMEDREIQNGGSLTV
                     RESQMAVFVNEGKIADVFAPGRYTLTTATLPVLTYLKNWDKLFKSPFKSDVYFFSTRQ
                     QVDQRWGTTQPVTIRDPDFGAVRLRAFGNYSYRISDPKLFHTEVSGTRDAYTVAELDG
                     QLRGLMLQHISDAVASSGIAFLDLAANQVEFAKLLMDATQPSFAALGLKLEAVTMQNV
                     SLPEELQKILDQKIGMGMVGGDMGKFMQYQTAQAIPKFAEGAAGGGGVVGDAMGLGAG
                     VALGQVMAQQLGQGLKTAPPETPAAAAAAGVRPEDVMATLEKLGELKTKGILTQDEFD
                     AKKAELLKKLV"
     gene            4359..>4863
                     /locus_tag="VFA35_01140"
     CDS             4359..>4863
                     /locus_tag="VFA35_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54802.1"
                     /translation="MHVAALHIAVIGAGPVGLALALQAARALPDSQITLFDARPAEKD
                     VAGDPRTLALSLGSVQLLERLGAWRADLAQPILEVHVSQQPPSLPALPGRLGEVFGEP
                     CLRIRAVDEAVPLLGAVLSYGSIVAPLQQAWLAAEAAEPRRLHSRFGQPVAALKNLVD
                     PENRAHGV"
BASE COUNT          741 a         1710 c         1625 g          787 t
ORIGIN      
        1 tccaacggac ctggccgacg aggcttcatg ttgtcggccg ggagcttggt caccacgcca
       61 ctgctcgccg gtatccctcg ttcggcctcc gcgcagcctg ctgccgcgag ccgcccgtcg
      121 acctcgacac ttggccgccg gacgctcggc gcgctcgagg tctcgagcct tgggctgggc
      181 gtgcagaaca tgagccggac ctatcagacc acggtgccga gccggcccga gatgatcaac
      241 atcatccgga ccgcgttcga ccggggcgtc accttcttcg atgcggcaga ggcttacggc
      301 ccgcacgagg tcgaacgcat cctcggtgaa ggcgtcgcac ccattcggga tcaggtcgtc
      361 atcgcgtcga agttcggctg ggacatcgac ctgaagaccg gtgagcgccg tgccgggctg
      421 aacagccggc cggatcacat caggctcgcc gtcgacggga tgctgacgcg gctgcgcacg
      481 gaccggatcg atctgctcta ccaacaccgc gtcgacccgg ccgtgcccat cgaggacgtt
      541 gccggcgccg tcaaggacct gatggcgcag ggcaaggtgt tgcactgggg tctgtgcgaa
      601 atgggcccga acacgctgcg ccgcgcgcat gcggaacagc gcgtcacggc agtgcagaac
      661 gaatactcca tgctttggcg cggccccgag aaatggctgc tgccgttgtg cgaggaactc
      721 ggtatcggct tcgtgccgtg gagcccgctc ggcgtcgggt tcctgacggg cgccatcgac
      781 gccaagacgc ggttcgctga cggtgacatc cgcaagggtg aatcgcggct ctcgccggag
      841 aacttgccgc acaacctggc gctcgtcgaa ctggtgaaac ggtgggcgca gcgcaagggc
      901 gccacgccgg cgcagacggc cctggcctgg ctgacggcgc aaaaaccatg gatcgtgccg
      961 atcccgggca cgacgcagat gccgcacatg ctggacaaca tcggcgcggc ggccatccgc
     1021 ttcacgtcgg cagaactcgt ggagctgaac gccgccgtgt cgatgatcca gattcgcggt
     1081 gctcgtcttc ccgacgctgt cttggctttc tcggacgtcg aagcgcctgc caagcgctga
     1141 catccgtgcg gctgcagtac ctcatgcaac tgccgtccgg gccagcgcct gtcgctggag
     1201 ctcaacgact gcttgatgcc aggcagcgtg agtacgcccg tcggcggcca ccatcagcaa
     1261 ccgctgcctt gctgtcgtcc aaaggtcagc aagggaccgc ccccgttccg gcggcgcagg
     1321 aggttccagc ttcgtatcac ccatgaaccg ccgccgcgac gatcaacccg atcgcgatgc
     1381 acatcgcgcc gacgacgatc gcgagcgcga cgttcttctt ctcgacgatc tcgccccaca
     1441 agtcgtaggg cgtgagcttg tcgacgatca cgaagcagat ccagaagatc gccacgccga
     1501 tcagcgcgaa cacgatcgag ccgacgatga cgccgggctt catccactca attcccatca
     1561 tcgcgaacct cctgtcgaag attgaacaag gcgccagttc atttgtggcc gccgccgctc
     1621 gagaacccgc cgaacgaacc gccgctgctg ccgtagctgc cgccgctgcg gttgctgttc
     1681 aggcaattct gatactcgct cgacgccgcg ccgaaggtgt cgcgcgtggc gtcgcagttg
     1741 ctcgaactgc tgctgccgca gcggaacagc agcagcacga cgaccacgac gaatacccag
     1801 aagaagatct tcgccagcag cgacgccggg ttcgacgagg tcggcagcgc atcgcgcgcc
     1861 agcgcggcac ccttgtcggg cgccaggcgg aaggccttca tcaccgcgtc ggccgagagc
     1921 gtctcgccgc ccgaccagac cacctcctcg gtgtcggcgg cggcggtctg ctcgcggttc
     1981 agccgcttcg ccgccgcgct gccggtgccc tggtagtcgg tgttgaccgt gcgctggttt
     2041 tgcgtgacct gccagtagaa ctcgccgagc acataggtga ccatgccggt gtagtcgtac
     2101 agcttgcggt agaccacgtt ctcgtacttc acgccgctgc cggcgcgctc gggcacgccg
     2161 gtgagcggcg cggtccagct ccagccgtcc tcggcgtcga cgatgaacgc gaagccctcg
     2221 gtgcggtggt agagcaggta ctcgcgccag aacgtacgct cgtcgtcgtc gctcgcaggc
     2281 gcctcgcagc gctcgacata gccgaccacc tgccacggca gcgcggcctt gccgagcgcc
     2341 agcgtgccgg tgctgccgag cgggatcagc ggcggcgtgc cgttggcctg cgcgtagtgc
     2401 tgcaactcgc cgccaatgcc gtgcgagaca tcgacgaccg catggcactg cgggcacacg
     2461 atggactgcg tcgtcgcgag cttcacgtcg agcgacgcac cgcagctcgg gcattcgatg
     2521 gccctgccct tcagcgtctt ctcggccggc tcggccaggc cggtcatcgc aagctcgctg
     2581 atcgcgaccg cgcggccgat cgaccagcgc ggcgcgcccg cttcgccgta gtcgagcgtg
     2641 ccgacctcgc cgcgtgtgtt gcgcaggtcg cagacggtga agccgcgatc ggtcaccggc
     2701 ggcgtcggca gctcgccttg cgcggcgatc acgctcgcag cgaccaccga cgcgacggtc
     2761 cacggctgcc ggtcgatcac gaccggcgcg ccggccttca ggctggtcgc gctcggcgcc
     2821 gcgccgtcga gcggcgcatc gaacgcgatc acgtagcggc cgttgtcttc gctcagccag
     2881 cccgacttgc cgttgccgaa caacgcatgc cattcgttcc aggtgccttc ggcgtagcgg
     2941 tactgcagcc ggccgacgag cgtgaacgcg gcgccctggt agcggccggc ggcgccgagc
     3001 tgcagcggcg agtggtcgtc gaacagctcg gcgctctggc cgatcctgcg cagcgcgtcg
     3061 ccctcgcgca cgaccgtgct cttgcagaag ctgcagacgg cgaacgccga cgcggccgat
     3121 cggaactcga ccggcgcgcc gcagttcggg cacgcagcgc ggtaggcgcg ctgcggtgag
     3181 ggctcggtag ccaagtgagg gcttgactac ggcgtcagac gagcttcttg agcagctccg
     3241 ccttcttcgc gtcgaactcg tcctgggtca ggatgccctt ggtcttgagt tcgccgagct
     3301 tctcgagcgt ggccatcacg tcctcgggcc ggacgccggc agccgccgcg gcggccggcg
     3361 tctcgggcgg cgccgtcttc agcccctggc cgagctgctg cgccatcacc tggccgagcg
     3421 cgacgcccgc gcccagcccc atcgcatcgc cgacgacacc gccgccgccg gccgcgccct
     3481 cggcgaactt cgggatcgcc tgcgcggtct ggtactgcat gaacttgccc atgtcgccgc
     3541 cgaccatgcc catgccgatc ttctggtcga ggatcttctg caactcctcg ggcagcgaga
     3601 cgttctgcat cgtcaccgct tcgagcttga gcccgagcgc ggcgaacgac ggctgcgtcg
     3661 cgtccatcag cagcttcgcg aactcgacct gattcgccgc gaggtcgagg aacgcgatgc
     3721 cgctggacgc gaccgcgtcg gagatgtgct gcagcatcag cccgcgcagc tggccgtcga
     3781 gctcggccac ggtgtaggcg tcgcgcgtgc ccgagacctc ggtgtggaag agcttcgggt
     3841 cgctgatgcg gtagctgtag ttgccgaacg cgcgcaggcg cacggcgccg aagtccgggt
     3901 cgcggatcgt caccggctgg gtcgtgcccc agcgctggtc gacctgctgg cgcgtgctga
     3961 agaagtagac atcgctcttg aaagggctct tgaagagctt gtcccagttc ttcaggtaag
     4021 tcaggaccgg cagcgtcgcg gtcgtcagcg tgtagcggcc gggtgcgaac acgtcggcga
     4081 tcttgccctc gttgacgaac acggccatct gcgactcgcg caccgtgagc gagccgccgt
     4141 tctggatctc gcggtcttcc atcgggtagc gccaggcgag cgtgccgtcg ccggactcgg
     4201 tccactcgat gatgtcgatg aactgtttct tgatgaaatc catcagcgcc atggcagcaa
     4261 actccttcgc gtcggaccgg gatccggggt gcggcgaata atacgccggc gccctgaact
     4321 tcaaccccca ccaacgggtg tctcccatga acgatcccat gcacgtcgca gccctccaca
     4381 tcgcggtgat cggcgccggc ccggtcggcc tcgcgctggc gctgcaggcg gcgcgcgcgc
     4441 tgcccgattc gcagatcacg ctgttcgacg cgcggccggc cgagaaggac gtggcgggcg
     4501 acccgcgcac gctcgcgctc tcgctcggca gcgtgcagct gctcgagcgg ctcggcgcgt
     4561 ggcgcgccga tctggcgcag ccgatcctcg aggtgcatgt gtcgcagcag ccgccctcgc
     4621 tgccggcgct tccgggtcgc ctcggcgagg tgttcggcga gccctgcttg cgcatccgcg
     4681 cggtcgacga ggccgtgccg ctgctcggtg cggtgctgag ctacggcagc atcgtcgccc
     4741 cgctgcagca ggcctggctc gccgccgagg ccgccgaacc gcgccggctg cacagccgct
     4801 tcggccagcc ggtcgcggcg ctgaagaacc tggtggaccc cgagaaccgg gcacacggcg
     4861 tcg
//
LOCUS       DASRDG010000068         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_246796, whole genome shotgun sequence.
ACCESSION   DASRDG010000068 DASRDG010000000
VERSION     DASRDG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246796"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFA35_01145"
     CDS             complement(<1..286)
                     /locus_tag="VFA35_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54803.1"
                     /translation="MSHARDQAYFIGLAAINLLHRVLPFALRPILYRLCRFDIASGAT
                     LQGGVRFFQIGRLAIGDGSLVNRGVYLDNRAGITIGRQVSIAHDARIYTMG"
     gene            complement(301..1695)
                     /locus_tag="VFA35_01150"
     CDS             complement(301..1695)
                     /locus_tag="VFA35_01150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZT54804.1"
                     /translation="MKITLIGSGYVGLVTGACLAELGNSVMCLDVDADKIALLNAGGV
                     PIHEPGLDDLIARNRAAGRLSFSADVAAAVAHGEIQFIAVGTPPDEDGSADLRHVLAA
                     AHNIGRHMTGFKVVVNKSTVPVGTADKVSEAITQALNARSFPTAAARPAFALVSNPEF
                     LKEGAAVEDFMRPDRIVVGVDDGAAGAHALALMRTLYAPFNRHHERTLVMDLKSAEFT
                     KYAANAMLATRISFMNELANLADRVGADIEFVRKGIGSDPRIGYGFLYAGTGYGGSCL
                     PKDVRALGRTAHEHGVELRVLRAVEAVNEGQKQVLVDKIVARFGADLTGRTFAIWGLA
                     FKPNTDDMREAPSRVVIDALVRRGARVQLHDPIAMVEARRCLESDLAAQPAALARVSY
                     APAQMDALRGADALVIVTEWKAFRSPDFGAIKAALAQPVIFDGRNLFEPAAMAAAGIE
                     YIAIGRGAPAGLAA"
     gene            1825..>3074
                     /gene="lysS"
                     /locus_tag="VFA35_01155"
     CDS             1825..>3074
                     /gene="lysS"
                     /locus_tag="VFA35_01155"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZT54805.1"
                     /translation="MSDENQLITERREKLAAIRQQGVAFPNDFRPKHHAAELLRQHGD
                     QENEELEPLAVEVVVAGRLMLKRVMGKASFGTLQDGSGQIQLFVTRDALGEDAYAAFK
                     HWDLGDIVGAEGLLFKTKTGELSVRVTKLRLLTKSLRPLPDKFHGMADQEQKYRQRYV
                     DLITDAEARGRFVARSKALASIRQFMVEHHFMEVETPMLHPIPGGANAKPFVTHHNAL
                     DQQMFLRIAPELYLKRLIVGGFERVFEINRSFRNEGVSVRHNPEFTMMEFYAAYWNHH
                     DLMDFTEEVLRHAARVATGSAAITYAGKPVALDQPFARLSVRDSLIKHAGLSEAEAGD
                     AAVLHAKLKALGAEPPAHWTLAELQFGLFEAAVEEKLWDPTFIIDYPVEVSPLARASD
                     ADPTITERFELFITGREYANGFSEL"
BASE COUNT          524 a         1071 c         1030 g          449 t
ORIGIN      
        1 ggcccatcgt gtagatgcgc gcatcgtgcg cgatcgacac ctgccggccg atcgtgatgc
       61 cggcgcggtt gtcgaggtag acgccgcggt tcaccaggct gccgtcgccg atcgcgagcc
      121 ggccgatctg gaagaagcgc acgccacctt gcagcgtcgc gcccgacgcg atgtcgaagc
      181 ggcacagccg gtacaggatc ggccgcagcg cgaacggcag cacgcggtgc aacaggttga
      241 tggccgccag cccgatgaag taggcctgat cgcgcgcgtg ggacatgcgg cgcgcctggg
      301 tcaggccgcg agcccggcag gcgcgccgcg cccgatcgcg atgtattcga tgcccgccgc
      361 cgccatcgcc gccggctcga acaggttgcg cccatcgaag atcaccggct gcgcgagcgc
      421 cgccttgatg gcgccgaaat cagggctgcg gaacgccttc cattcggtga cgatgacgag
      481 cgcgtcggcg ccgcgcagcg catccatctg cgccggcgcg tagctcacgc gcgccagcgc
      541 cgccggctga gcagccagat cggattcgag gcagcgccgc gcctcgacca tcgcgatcgg
      601 atcgtgcaac tgcacgcgcg cgccgcgccg caccagcgcg tcgatcacga cgcggctcgg
      661 cgcctcgcgc atgtcgtcgg tgttgggctt gaaggccagg ccccagatgg cgaaggtgcg
      721 cccggtcaga tcggcgccga agcgggccac gatcttgtcg acgagcacct gcttctggcc
      781 ctcgttgacg gcctcgacgg cacgcagcac gcgcaactcg acgccgtgtt cgtgcgcggt
      841 gcggccgagc gcacgcacgt ccttgggcag gcacgagccg ccgtagccgg tgccggcata
      901 gagaaaaccg tagccgatgc gcgggtcaga gccgatgccc ttgcgcacga actcgatgtc
      961 tgcgccgacg cgatcggcca ggttcgcgag ttcgttcatg aacgagatgc gcgtggccag
     1021 catcgcgttc gccgcgtact tggtgaactc ggccgacttc aggtccatca ccagcgtgcg
     1081 ttcgtggtgg cggttgaacg gcgcgtacag ggttcgcatc agcgcaagcg cgtgagcgcc
     1141 cgcggcgccg tcgtcgacgc cgacgacgat gcggtccggc cgcatgaagt cttcgaccgc
     1201 ggcaccttcc ttcaggaact ccgggttcga caccagcgcg aacgccggcc gcgccgcggc
     1261 ggtcgggaac gagcgcgcat tcagcgcctg cgtgatggcc tcgctcacct tgtccgccgt
     1321 gcccaccggc acggtcgact tgttgacgac caccttgaag cccgtcatgt ggcggccgat
     1381 gttgtgcgca gccgcgagca cgtggcgcag atcggccgag ccgtcttcgt cgggcggcgt
     1441 gcccacggcg atgaactgaa tctcgccgtg cgcgacagcc gccgcgacat cggccgagaa
     1501 actcagccgg cccgccgcgc ggttgcgcgc gatcaggtcg tccagccccg gctcgtggat
     1561 cgggacacca ccggcattga gcagcgcgat cttgtcggcg tcgacatcca ggcacatgac
     1621 gctgttgccg agttcggcca gacacgcgcc cgtcaccagg ccgacataac ccgaacctat
     1681 caaagtgatc ttcatgggga gagggttggg agaggggcaa gagagcgcgg gcccgattct
     1741 atcgagacac cccagcgtgc ctcgcgcggc gcccgcgcgg gcaggcgcga gccccctaaa
     1801 atcgcgcctt catcgaaagc caccatgtcc gacgaaaacc agctcatcac cgagcgccgc
     1861 gagaagctcg ccgccatccg ccagcaaggt gtcgcgttcc cgaacgactt ccgccccaag
     1921 caccacgccg ccgagttgct gcgccagcac ggcgaccagg agaacgagga actggagccg
     1981 ctcgcggtcg aggtggtcgt cgccgggagg ctgatgctca agcgtgtgat gggcaaggcg
     2041 agcttcggca cgctgcagga cggctcgggc cagatccagc tgttcgtcac gcgcgacgcg
     2101 ctcggcgaag acgcctacgc ggccttcaag cactgggacc tgggcgacat cgtcggcgcc
     2161 gaaggcctgc tcttcaagac caagaccggc gagctctcgg tgcgcgtgac gaagctgcgc
     2221 ctgctgacca agagcctgcg cccgctgccc gacaagttcc acggcatggc cgaccaggag
     2281 cagaagtacc gccagcgcta tgtcgacctg atcaccgacg ccgaggcgcg cggccgcttc
     2341 gtcgcgcgca gcaaggcgct cgcgtcgatc cgccagttca tggtcgagca ccacttcatg
     2401 gaagtggaaa cgccgatgct gcacccgatc ccgggcggcg cgaacgcgaa gcctttcgtc
     2461 acgcatcaca acgcgctcga tcagcagatg ttcctgcgca tcgcgccgga gctgtacctg
     2521 aagcggctga tcgtcggcgg cttcgagcgc gtgttcgaga tcaaccggag cttccgcaac
     2581 gaaggcgtgt cggtgcggca caaccccgag ttcacgatga tggagttcta tgccgcgtac
     2641 tggaaccacc acgacctgat ggacttcacc gaagaggtgc tgcgccacgc cgcgcgcgtc
     2701 gcgaccggtt cggccgcgat cacctacgcg ggcaagccgg tggcgctcga ccagccgttc
     2761 gcgcgcctgt cggtgcgcga ttcgctcatc aaacacgcgg gcttgagcga agccgaggcc
     2821 ggcgacgccg ccgtgctgca cgcgaagctg aaggcgctcg gcgcggagcc accagcgcac
     2881 tggacactgg ccgagttgca gttcggcctg ttcgaggcgg cggtcgaaga gaagctctgg
     2941 gacccgacct tcatcatcga ctatccggtc gaagtctcgc cgctggcgcg cgcctccgac
     3001 gccgacccga cgatcaccga acgcttcgag ctcttcatca ccgggcgcga atacgccaac
     3061 ggcttctccg agct
//
LOCUS       DASRDG010000069         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_471100, whole genome shotgun sequence.
ACCESSION   DASRDG010000069 DASRDG010000000
VERSION     DASRDG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471100"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFA35_01160"
     CDS             complement(<1..407)
                     /locus_tag="VFA35_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT54806.1"
                     /translation="MRAPRPLLLCVALLLAAAAVAACSPLRLLNAIAAAGGGYRVDSA
                     LAYGALARQRLDVYTPTAAAPAGGWPVVVFFYGGAWNSGERAEYGFVGSALAARGVLT
                     LVADYRLYPEVRFPEFLRDSARALAWGLTEAARL"
     gene            complement(415..1830)
                     /gene="argH"
                     /locus_tag="VFA35_01165"
     CDS             complement(415..1830)
                     /gene="argH"
                     /locus_tag="VFA35_01165"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZT54807.1"
                     /translation="MSTNQFDKKSEAWSALFSEPMSELVKRYTASVGFDQRLWRADIA
                     GSLAHAEMLAAQGIISAADHAAIQRGMAQIAAEIEAGSFEWKLDLEDVHLNIEARLTS
                     LVGDAGKRLHTGRSRNDQVATDVRLWLRDEIGAIAGLLTALQRVLVETAEKNAEVILP
                     GFTHLQVAQPVSFGHHLLAYVEMFARDAERMGDVYRRTNRLPLGAAALAGTSYPLDRE
                     RVARTLGMVDDQGAACVCQNSLDAVSDRDFAIEFTAAAALCMIHVSRFSEELVLWMSQ
                     NFGFIDLADRFCTGSSIMPQKKNPDVPELARGKTGRVVGHLMGLITLMKGQPLAYNKD
                     NQEDKEPLFDTVDTLKDTLRIFAEMVGGITVKPEAMEAAALRGYATATDLADYLVKKG
                     LPFRDAHEVVAHAVKLAIAQGADLSALPLATLQGFHAAIGADVYEVLSLRGSLNARNL
                     LGGTAPAQVRAQVARHRARLG"
     gene            1892..2989
                     /locus_tag="VFA35_01170"
     CDS             1892..2989
                     /locus_tag="VFA35_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZT54808.1"
                     /translation="MPARPPLPDTLAPTRPGELGRSSGFGTTMFDALAPERAGAAPNS
                     VFDVCHVGVVLRALLFVHGVLAIGVLFAAGSVVGWVTALATGSSVALPAVLVWLLLVC
                     AGRRPFGALPAAGQWAVAIALGALSGAASALLAWFAAGDPLVAPAWLAPALAGAALAA
                     AMFQWLQLRAQALLPADTAARLAELQSRIRPHFLFNTLNTALALVRHDPARAEAVLED
                     LSELFRVAITENAESVSLAEEVALAQRYLAIEQVRFGSRLQVSWELDPEAGAARVPPL
                     LLQPLVENAVRHGIEPDAAGGAIRVRTRVKLGRALVSIVNSVPAAASAPGNGIALRNV
                     RERLRLMHDVAAQFETRRETDVFRVQIVVPL"
     gene            3120..3902
                     /locus_tag="VFA35_01175"
     CDS             3120..3902
                     /locus_tag="VFA35_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZT54809.1"
                     /translation="MIRVLVVDDEELARLRLRGLVHDSVEPKAGVVGEAANAAQALAW
                     LTDNSCDLILLDVQMPGRDGMQLAAELKRRPAPPAIVFVTAHVQHALQAFDLDAVDYL
                     TKPVRRERLHAALQRVAQRLALQRGVGAAAPPPAGAEDAPAIVVTDRGRVLRVPLAEV
                     LYFKAELKYVTLRTAEHTYVLDEALSDLEQRLGERFLRVHRNALVARAAVRALERRVV
                     AGEHDEEGAEGWAVRIAPVDEWLAVSRRQVAAVREALVAAGR"
BASE COUNT          510 a         1392 c         1471 g          592 t
ORIGIN      
        1 aggcgcgccg cttcggtcag gccccaggcg agcgctcgcg cgctgtcgcg caggaactcg
       61 ggaaagcgca cctcggggta gagccggtag tcggccacca gggtcagcac gccgcgcgcg
      121 gccagtgcgc tgccgacgaa gccgtactcg gcgcgctcgc cgctgttcca cgccccgccg
      181 tagaagaaca cgacgaccgg ccagccaccc gccggcgcgg ccgcggtcgg cgtgtagaca
      241 tcgagtcgtt ggcgcgcgag cgcgccatac gcgagcgccg aatcgacgcg gtagccgccg
      301 ccggcggcgg cgatggcatt gagcagccgc agcggtgagc aggcggcgac cgccgccgcg
      361 gccagcagca gggcgacgca aagcagcagg ggcctgggtg ctcgcaaggg cgcgctaccc
      421 gagccgcgcc cgatgccgcg cgacctgcgc gcgcacctgc gccggcgcgg tgccgccgag
      481 cagattgcgc gcgttcagcg agccgcgcaa gctcagcacc tcgtacacgt ccgcgccgat
      541 cgcggcgtga aaaccttgca gcgtggcgag cggcaacgcg ctgagatcgg cgccttgcgc
      601 gatcgcgagc ttgacggcgt gcgcgaccac ttcatgcgcg tcgcggaacg gcaggccctt
      661 cttgaccagg tagtcggcca ggtcggtcgc ggtggcatag ccgcgcaggg cggcggcttc
      721 cattgcctcg ggcttgaccg tgatgccgcc gaccatctcg gcgaatatgc gcagtgtgtc
      781 cttcagcgtg tcgacggtgt cgaacagcgg ttccttgtct tcctggttgt ccttgttgta
      841 ggccagcggc tggcccttca tcaaggtgat caggcccatc aggtggccga cgacgcggcc
      901 ggtcttgccg cgcgcgagct cgggcacgtc ggggttcttc ttctgcggca tgatcgaact
      961 gccggtgcag aagcgatcgg ccaggtcgat gaagccgaag ttctggctca tccacagcac
     1021 cagctcttcg ctgaagcgcg agacgtggat catgcacagc gccgcggccg cggtgaactc
     1081 gatcgcgaag tcgcggtcgc tcaccgcgtc gaggctgttc tggcagacgc aggctgcgcc
     1141 ttggtcgtcc accatcccca gcgttcgggc gacgcgctcg cggtccagcg ggtagctggt
     1201 gccggccagc gccgccgcgc cgagcggcag gcggttggtg cggcggtaca catcgcccat
     1261 gcgctcggca tcgcgcgcga acatctcgac atacgcgagc aggtggtgcc cgaagctcac
     1321 cggctgagcc acctgcaggt gcgtgaagcc gggcaggatc acctcggcgt tcttctcggc
     1381 ggtctcgacc agcacacgct gcagcgcggt cagcaggccg gcgatcgcgc cgatctcgtc
     1441 gcgcagccac agccgcacgt cggtcgcgac ctggtcgttg cgcgagcggc cggtgtgcag
     1501 gcgtttgccg gcatcgccga ccaggctggt caggcgcgcc tcgatgttca ggtgcacgtc
     1561 ttcgaggtcg agcttccact cgaagctgcc ggcctcgatc tcggcggcga tctgcgccat
     1621 gccgcgctgg atcgccgcgt gatccgcggc gctgatgatg ccctgcgccg ccagcatctc
     1681 ggcgtgcgcg aggctgccgg cgatgtcggc ccgccacagc cgctggtcga agccgacgct
     1741 cgcggtatag cgcttgacca actcgctcat cggctcggaa aacagggccg accaggcttc
     1801 ggattttttg tcgaattggt tggtggacat gcgggtgacg ctggacaatg cgagtcacgg
     1861 attctatcga ggcccccgcc agagaacgtc gatgcccgcc cgcccgccgc tccccgacac
     1921 gctcgcgccg acccggccgg gcgagctggg ccgttcgagc ggtttcggca cgacgatgtt
     1981 cgatgcgctc gcgcccgagc gggccggcgc ggcgccgaac tcggtgttcg acgtctgcca
     2041 tgtcggcgtg gtgctgcgcg cgctgttgtt cgtgcacggc gtgcttgcga tcggcgtgct
     2101 gttcgctgcc ggctcggtcg tcggctgggt cacggcgctc gcgaccggtt cgagcgtggc
     2161 gctgccggcg gtgctggtgt ggctgctgct ggtgtgcgcc ggccgccggc cgttcggcgc
     2221 gctgcccgcc gccgggcaat gggcggtggc gatcgcgctc ggcgcgttga gtggtgcggc
     2281 cagcgcgctg ctggcctggt tcgccgccgg cgacccgctg gtcgcgccgg cctggctcgc
     2341 gcccgcgctg gcgggcgcgg cgctggcggc ggcgatgttc cagtggctgc agttgcgcgc
     2401 gcaggcactg ctgccggccg acacggccgc gcggctggcc gagttgcagt cgcgcatccg
     2461 cccgcacttc ctgttcaaca ccttgaacac cgcgctcgcg ctggtgcgcc acgacccggc
     2521 gcgcgccgag gcggtgctcg aagatctgtc cgaactgttc cgcgtcgcga tcaccgagaa
     2581 cgccgagtcg gtctcgctcg ccgaggaggt cgcgctcgcg cagcgctacc tcgcgatcga
     2641 gcaggtgcgg ttcggcagcc gcctgcaggt cagctgggag ctcgaccccg aggccggcgc
     2701 cgcgcgcgtg ccgccgctgc tgctgcagcc gctggtcgag aacgcggtgc gccacggcat
     2761 cgagcccgac gcggcgggcg gcgcgatccg cgtgcgcacg cgggtcaagc tcgggcgcgc
     2821 gctggtgtcg atcgtcaaca gcgtgccggc cgcggcctcg gcgcccggca acggcatcgc
     2881 gctgcgcaac gtgcgcgagc gcctgcggct gatgcacgac gtggcggcgc agttcgagac
     2941 gcggcgcgag accgacgtgt ttcgcgtgca gatcgtggtg cctttatgac cgcccctcgc
     3001 cccgcgggta cgcgggtcga tcccctcggg ccacgaaggg gcccctgcgt ggcccccgga
     3061 tcgggccttg cttggggcgg cccggcgcgg cggcccggtt cgccgcgtgg agccggctca
     3121 tgatccgcgt gctggttgtc gacgacgaag agctcgcgcg gctgcggctg cgcggcctgg
     3181 tgcacgacag cgtcgaaccg aaggccggtg tcgtcggcga ggcggcgaac gccgcgcagg
     3241 cgctcgcctg gctgaccgac aacagctgcg acctgatcct gctcgacgtg cagatgccgg
     3301 gccgcgacgg catgcaactc gctgcggaac tgaaacgccg gcccgcgccg ccggccatcg
     3361 tgttcgtgac cgcgcatgtg cagcacgcgc tgcaagcctt cgacctcgac gcggtcgact
     3421 acctgaccaa gccggtgcgg cgcgagcggc tgcacgcggc gctgcagcgg gtcgcgcagc
     3481 ggctcgcgct gcagcgcggc gtcggcgccg ccgcgccgcc gccggccggg gcggaggacg
     3541 cgccggcgat cgtcgtcacc gaccgcggcc gggtgctgcg cgtgccgctg gccgaggtgc
     3601 tgtacttcaa ggccgagctg aagtacgtga ccctgcgcac ggcagaacac acctacgtgc
     3661 tcgacgaagc gctgagcgat ctggagcagc gcctgggcga gcgctttctg cgcgtgcacc
     3721 gcaacgcgct ggtcgcacgc gccgcggtgc gtgcgctcga gcgccgggtg gtcgccggcg
     3781 aacacgacga agagggcgcc gaaggctggg ccgtgcgcat cgcgccagtc gacgaatggc
     3841 tcgcggtctc gcgccggcaa gtggccgcgg tgcgcgaggc attggtggcc gcggggcggt
     3901 gaacgccggc tcagcccgtg ttgcgcaacc ccgtcgccac gccgttgatc gagatgtgga
     3961 tgccg
//
LOCUS       DASRDG010000070         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1165212, whole genome shotgun sequence.
ACCESSION   DASRDG010000070 DASRDG010000000
VERSION     DASRDG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165212"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VFA35_01180"
     CDS             <1..698
                     /locus_tag="VFA35_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559457.1"
                     /note="catalyzes the reversible formation of glyoxylate
                     and succinate from isocitrate; glyoxylate bypass pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZT54810.1"
                     /translation="REAVAKLVAARLAADVSGTPTLVIARTDAEAADLVTSDVDDIDK
                     PFLTGERTVEGFYRTRAGLDQAVSRGLAYSEVADMVWCETGKPDLAYAKAFAEAIHRK
                     FPGKLLAYNCSPSFNWKKNLDDATIAKFQRELGAMGYKFQFITLAGFHSLNYSMFNLA
                     HGYARNNMSAFVELQEAEFAAADRGFTAVKHQREVGTGYFDAVTTTIEALSSTAALKG
                     STEDEQFFDAKHG"
     gene            901..2421
                     /locus_tag="VFA35_01185"
     CDS             901..2421
                     /locus_tag="VFA35_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HZT54811.1"
                     /translation="MPAFAARFALCAVFAWPIAAQAVNFKDVEVRARALAAENFRPRA
                     AKLPPALAALDYDALRDIRFRPDRATWRADKLPFELMYFHPGKNFPDPVRINLADARG
                     VKRVDFDTELFDYGRNKLDPNKLRGVGLAGFRVHYPVNRSNYKDEVLVFLGASYFRAI
                     GKGQTYGLSARGLAIDTAVPSGEDFARFTEFWIDRPGRADTALTIYALLDAKHLTGAY
                     KFVLTPGAETVVQVSARLFFREAVAKLGLAPLNSMYFFGDNQPGGRDDYRPEVHDSDG
                     LSVAYDNGDWIWRPLVNPKRLLVTSFAATNPKGFGLMQRDRAPASYEDPEALYERRPS
                     AWIEPVGKWGAGRVELVQIPTPDETNDNIVAYWVPDKTPDPRRPFDFAYTMRWQLNSQ
                     APNGKAWVVQTRRGRGYVKKPDGDLNFVVDFDGPPLRDLKPDAKPEAVVEVGGNAQLR
                     EKNLFLNPATGVWRMTVRVKREDAAKPIEMRGSLREGGRSLTETWSYIVPPESDKP"
BASE COUNT          446 a          913 c          837 g          373 t
ORIGIN      
        1 cccgtgaagc ggttgccaag ctcgttgcgg cgcgcctcgc agccgacgtc agcggcacgc
       61 cgaccctcgt gatcgcacgc accgatgccg aagctgccga cctcgtgacc agcgacgtcg
      121 acgacatcga caagccgttc ctgaccggcg agcggacggt cgaggggttc tatcgcacgc
      181 gtgccggact ggatcaggcg gtgtcgcgcg ggctggccta ttcggaagtc gccgacatgg
      241 tctggtgcga gaccggcaag cccgacctgg cctacgccaa ggccttcgcc gaggcgatcc
      301 accgcaagtt ccccggaaag ctcctggcct acaactgctc gccgagcttc aactggaaga
      361 agaacctcga cgacgccacc atcgccaagt tccagcgcga gctcggcgcc atgggctaca
      421 agttccagtt catcaccctg gccggcttcc atagcctgaa ctactcgatg ttcaacctgg
      481 cccacggcta tgcgcgcaac aacatgagcg cgttcgtcga actgcaggaa gccgagttcg
      541 cggcagccga ccgcggcttc accgccgtga agcaccagcg cgaggtcggc accggctact
      601 tcgacgccgt cacgacgacg atcgaagcgc tgtcgtcgac ggccgcgctg aagggttcga
      661 ccgaagacga gcagttcttc gacgccaaac acggttgacc gacggctgac ccacgaccgg
      721 gcgcggcgcg agacggcgcc gcgcccggtc gcgcaagtaa gcaaagcaat ccggcccggc
      781 gcagccggga aataccttta gagttctggc cttatcggca tgggattcca ccggcagaat
      841 cccggtgcat cccctgcgcg cttcaggaag ggccagactc tgaatttcgt tcccgctttc
      901 ttgccggcat tcgccgcacg cttcgcgctg tgcgctgtct tcgcgtggcc gatcgccgca
      961 caggcggtca acttcaagga tgtcgaggtg cgggcgcgcg cgctcgcggc cgagaacttt
     1021 cgcccgcgcg ccgcgaagct gccgcccgcg ctggccgcac tcgactacga cgcgctgcgc
     1081 gacatccgct ttcgccccga ccgcgcgacc tggcgcgccg acaagctgcc gttcgagctg
     1141 atgtacttcc accccggcaa gaactttccg gacccggtgc gcatcaacct cgccgacgcg
     1201 cgcggcgtga agcgcgtcga cttcgacacc gagctgttcg actacggccg caacaagctc
     1261 gatccgaaca agctgcgcgg cgtcgggctg gccgggtttc gcgtccacta cccggtcaac
     1321 cgcagcaact acaaggacga ggtgctggtc ttcctcggcg cgagctactt ccgcgcgatc
     1381 ggcaagggcc agacctacgg cctgtcggcg cgcggtctgg cgatcgacac ggccgtgccg
     1441 tcgggcgaag acttcgcgcg cttcaccgag ttctggatcg accgtcccgg ccgcgccgac
     1501 accgcgctga cgatctacgc gctgctcgat gcgaagcacc tgaccggcgc gtacaagttc
     1561 gtcctgacgc cgggcgccga gacggtggtg caggtgagtg cacgcctgtt ctttcgcgag
     1621 gcggtcgcca agctcgggct tgcgccgctg aacagcatgt acttcttcgg cgacaaccag
     1681 cccggcggcc gcgacgacta ccgccccgag gtgcacgact ccgacggtct ttcggtcgcc
     1741 tacgacaacg gcgactggat ctggcgcccg ctcgtcaacc cgaagcgcct gctcgtgacc
     1801 tcgttcgccg cgaccaaccc gaagggcttc ggcctgatgc agcgcgatcg cgcgccggcc
     1861 agctacgagg accccgaagc gctgtacgag cgccggccca gcgcctggat cgagccggtg
     1921 ggcaagtggg gcgccggccg ggtcgagctg gtgcagatcc cgacgcccga cgagaccaac
     1981 gacaacatcg tcgcttactg ggtgcccgac aagacacccg acccgcggcg gccgttcgac
     2041 ttcgcgtaca cgatgcgctg gcagctgaac tcgcaagcgc ccaacggcaa ggcctgggtc
     2101 gtgcagacgc ggcgcggccg cggctacgtg aaaaagcccg acggcgacct gaacttcgtc
     2161 gtcgacttcg acggcccgcc gctgcgcgat ctgaagcccg acgcgaagcc cgaggcggtc
     2221 gtcgaggtcg gcggcaacgc gcagctgcgc gagaagaacc tgttcctgaa ccccgccacc
     2281 ggcgtgtggc gcatgacggt gcgcgtgaag cgcgaagacg ccgccaagcc gatcgagatg
     2341 cgcggcagcc tccgcgaagg cggccgcagc ctgaccgaga cctggagcta tatcgtgccg
     2401 ccggagtcgg acaagccatg agcggaggca cacgagcggt cgagcgcggc atcgagccga
     2461 tgccaccgat gcaccgcgtt tcgatggccg agcagcgctg ggccgggcac ccactgtggc
     2521 ggccggtgcg gcgcctgctc gaagggcccg cgccgcgcac ctgggacaa
//
LOCUS       DASRDG010000071         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1046235, whole genome shotgun sequence.
ACCESSION   DASRDG010000071 DASRDG010000000
VERSION     DASRDG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046235"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..882
                     /locus_tag="VFA35_01190"
     CDS             100..882
                     /locus_tag="VFA35_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT54812.1"
                     /translation="MNNLNNAAAIAAPPRLLQWLEWRALGEMAATAFAWPTLALAPRG
                     DGHPVLVLPGLVASDRSTVLLRGFLRDRGYEVHGWGQGRNYGPRDGVREAMLAQLDQL
                     HASSGRKVSLVGWSLGGLYARTLARERPDAVRNAIMLGSPIGGQPRATNAWRLYELTS
                     GRSADDAEDWARSGEAGSVPATSIWSRSDGVVAWRNSLLAPGKRAENISVCGSHLGLG
                     ANAAVLYAVADRLAQPEGEWQPFKAPAMLRALYPKVDAPSQP"
     gene            879..1661
                     /locus_tag="VFA35_01195"
     CDS             879..1661
                     /locus_tag="VFA35_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZT54813.1"
                     /translation="MTRKVALVTGSATGVGAATALALAGRGYNVLVNYSKSDSEARAS
                     AAACEGAGADTLLVRGDVAQDSDCRALASAAVERWGRIDVLVNNAGVTTFAGSEAWDA
                     IDAATFQRVIGVNAAGTFQMIRACAPHLKAARGAIVNISSIAGALGIGSSVAYIASKG
                     AVNSMTLYFARALAPEVRVNAVCPGLITTRWFADGIGRDGYEAVKDGYERMAPLQSAS
                     SAEDVADAVVWLADGARTMTGELLLIDNGMHLGAKPLTGRPD"
     gene            complement(1677..>2397)
                     /locus_tag="VFA35_01200"
     CDS             complement(1677..>2397)
                     /locus_tag="VFA35_01200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZT54814.1"
                     /translation="SSGLGENFARLAAGCKAKVVIAARRKARLDTLAKELDRLGSPQV
                     TVIEMDVASEQSIDDAFAAIDASGDILDVVVNNAGVSGDGASLTLPTKDFDDLMAINV
                     RGVWLVAVRAARRWKEAGRGGSIVNIASIQGERVMPGITPYSSSKAAVVHMTKSLALE
                     WARYGIRVNAIEPGYIGTEMTDAMWETDHGKALIKRIPMRRLGKPEELDGLFLLFATE
                     AGSWMTGACVPVDGGHLCSSL"
BASE COUNT          360 a          874 c          824 g          339 t
ORIGIN      
        1 cgtcagtgtc aatccgactt cacacgcgcg gtcgacttgc gacacgatcc gcagcgaccc
       61 ttgttttttc agtaccgagc caaaaaagga gactgcacga tgaacaacct gaacaacgcc
      121 gccgcgatcg ctgcgccacc gcgcctgctt cagtggctgg agtggcgcgc cctgggcgag
      181 atggccgcca ccgcgttcgc atggcccacg ctcgcgctcg cgccgcgcgg cgacggccac
      241 ccggtgctgg tgctgcccgg cctcgtcgcc agcgaccggt ccacggtgct gctgcgcggc
      301 ttcctgcgcg accgcggcta cgaggtgcac ggctggggcc aggggcgcaa ctatggcccg
      361 cgcgacggcg tgcgcgaggc gatgctcgcg cagctcgatc agctgcatgc atcgagtggg
      421 cgcaaggtga gcctggtggg ctggagcctg ggcggcctgt atgcgcgcac gctcgcgcgc
      481 gaacggcccg acgcggtgcg caacgcgatc atgctcggca gcccgatcgg cggccagccg
      541 cgggccacca acgcgtggcg cttgtacgag ctgacgagcg gccgttccgc cgacgacgcc
      601 gaagactggg cacgcagcgg cgaggccggt tcggtgccgg ccacttcgat ctggagccgc
      661 agcgacggcg tcgtggcctg gcgcaacagc ctgctggccc ccggcaagcg ggccgagaac
      721 atcagcgtct gcggcagcca tctcggcctc ggcgccaatg ccgcggtgct gtacgcggtg
      781 gccgaccggc tcgcccagcc cgagggcgag tggcagccat tcaaggcgcc ggccatgctg
      841 cgcgcgctgt acccgaaggt cgacgcaccg agccagccat gacacgcaag gtcgccctcg
      901 tcaccggctc ggcgaccggc gtcggcgccg ccaccgcact ggccctcgcg ggccgcggct
      961 acaacgtgct cgtcaactac tcgaagagcg actccgaggc ccgcgcgtcg gcggccgcgt
     1021 gcgaaggcgc cggcgccgac acgctgctgg tgcgcggcga tgtcgcacaa gacagcgact
     1081 gccgcgcgct cgcgagcgca gctgtcgaac gctgggggcg catcgacgtg ctcgtcaaca
     1141 acgccggcgt gaccacattc gccggctccg aggcctggga cgcgatcgat gcggcgacct
     1201 tccagcgcgt catcggcgtc aacgccgccg gcacgttcca gatgattcgc gcctgcgcgc
     1261 cgcacctcaa ggccgcgcgg ggcgccatcg tcaacatctc gtcgatcgcc ggtgcgctcg
     1321 gcatcggctc gtcggtggcg tacatcgcgt cgaagggcgc ggtcaactcg atgacgctgt
     1381 acttcgcgcg cgccctcgcc cccgaggtgc gcgtcaacgc cgtgtgcccg gggctgatca
     1441 cgacccggtg gttcgccgac ggcatcggcc gcgacggcta cgaggcggtg aaagacggct
     1501 acgagcgcat ggcgccgctg caatcggcaa gcagcgccga agacgtcgcc gacgcggtcg
     1561 tctggctggc cgacggcgcg cgcacgatga ccggcgagct gctgctcatc gacaacggca
     1621 tgcacctcgg cgccaagccc ctcaccgggc ggcctgatta gcttcggcgc gcggtttcac
     1681 agcgacgagc agaggtgccc gccgtcgacc ggcacgcagg cgcccgtcat ccacgagccg
     1741 gcctcggtcg cgaacaacag gaacaagccg tcgagttcct cgggcttgcc gagccggcgc
     1801 atcgggatgc gcttgatcag cgccttgccg tggtcggtct cccacatcgc gtcggtcatc
     1861 tcggtgccga tgtaccccgg ctcgatcgcg ttgacgcgga tgccgtagcg cgcccactcg
     1921 agcgccaagc tcttggtcat gtgcacgacc gcggccttcg agctcgagta cggcgtgatg
     1981 cccggcatca cgcgctcgcc ctggatcgac gcgatgttga cgatcgagcc gccgcggccg
     2041 gcctccttcc agcgccgcgc cgcacgcacc gcgaccagcc agacgccgcg cacgttgatc
     2101 gccatcaggt cgtcgaagtc cttggtgggc agcgtcagcg acgcgccgtc gcctgagacg
     2161 ccggcgttgt tgacgacgac atcgagaatg tcgcccgagg cgtcgatcgc ggcgaaggcg
     2221 tcgtcgatcg actgctcgga cgccacgtcc atctcgatca cggtcacctg cggcgagccg
     2281 agccgatcga gctccttggc gagcgtgtcg agcctggcct tgcgccgcgc cgcgatcacc
     2341 accttggcct tgcaaccggc ggccaggcgg gcgaagttct cgcccaaccc cgaagac
//
LOCUS       DASRDG010000072         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_471458, whole genome shotgun sequence.
ACCESSION   DASRDG010000072 DASRDG010000000
VERSION     DASRDG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471458"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..>2542)
                     /locus_tag="VFA35_01205"
     CDS             complement(168..>2542)
                     /locus_tag="VFA35_01205"
                     /inference="COORDINATES: protein motif:HMM:NF027825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HZT54815.1"
                     /translation="TVPPAVVPRETPIDPDAPDPAAHQTALDRFRLSEEAVHDQRARE
                     LDDLRFVDEKGAQWDDDIKRLRGGQDAGSGLPAVPARPCLEFNLLRGPVQQVINTARQ
                     AKLGLSFAPEGEGASQLVAQAYDDIARAIQADSRAHLARQWAFERAAKCGFGVYRILT
                     EYVNTQTFDQRIIYKRVLNQASAYLDPFAQEPDWSDGQFALLTQDLPLARYKRAHPTS
                     TLAAYSDRELTALGDDIPQWITTSHGDAGVGVRVAEYWEVREETDVLVLLPDNTTARE
                     AEIPATILAQVERDRGAKLPRRTVTRGRRVFWSLINGVEILQGPQEWNGAYIPIIPVI
                     GDEANLNGDRRWTGIVQFARDAQQSYNYMRSAQVEAVGLAPRAQWLIADGQLEGYEAW
                     WQQANTRNLPYLPYRLTTYSGGQAPPPSRNVAEPAIQAVTLAAAAAKDDLHGTTNMPP
                     VSLGQLDPHERSGVAIRALQGQAEVGSSGYLDNLASVSMAYEGKVLKDLIPRVYDRPG
                     RVVPALGLDDKRRSLMVNIPFVEEGGQPRAVPPGTPGAQQIALAGAELSVTATVGKSY
                     ATRREETAAAITAVMQAAPGLAPILAPYWLDELDFPGAKKLAAIAKKTLPPQFQQDEG
                     QGQGPNPAQLQQQLAQAQQLIELLTKALQEKTQQIETDQVKADAQLQQTQLELASQER
                     IATLNAQVDVLKAELSAQGAKASTTVGALASLDRAALTAQAGTERALWQGVMRSVQQL
                     DQQAFLKEQQAAQLQAEREAQEAARAQQAQGPGPGAGGPPGAGAMEPGGA"
BASE COUNT          297 a          909 c          906 g          430 t
ORIGIN      
        1 ccggcgggcg caggcgagtc gggcgcgggc tcgctagctg gtgcgctcga cggcgcggac
       61 ggtgtgccgg acggcgcctc catctcggtg cgcaggtccg actcgctcgc ggtgttggtc
      121 gtgatggtct gcgcgccgtg cgtaacgctg acggaatcag gcatacatta cgcgcccccc
      181 ggctccatcg cgcccgcacc cggcggccca cccgcgcccg gccccggccc ctgcgcctgc
      241 tgcgcgcgcg ccgcctcctg cgcctcccgc tcggcctgga gctgcgccgc ctgctgctcc
      301 ttcaaaaacg cctgctgatc gagctgctgc acgctccgca tcacgccctg ccacaaggcg
      361 cgctccgtcc cggcctgcgc ggtgagcgcg gcgcggtcga gcgacgccag cgcgcctacc
      421 gtggtcgacg ccttcgcgcc ctgcgcggag agctcggcct tgagcacgtc gacctgcgcg
      481 ttgagcgtgg cgatccgctc ctgactcgcg agctccagct gtgtctgctg caactgcgcg
      541 tcggccttga cctggtccgt ctcgatctgc tgcgtcttct cctgcagcgc cttcgtgagc
      601 agctcgatga gttgctgcgc ttgcgccagc tgctgctgca gttgcgcggg gttcgggccc
      661 tgcccctggc cctcgtcctg ctggaactgc ggcgggagcg tcttcttggc gatggcggcc
      721 aacttcttgg cccccgggaa gtcgagctcg tcgagccagt agggcgccag aatcggcgcg
      781 aggcccggcg cggcctgcat gaccgccgtg atcgcggcgg cggtttcttc gcgccgcgtc
      841 gcgtagcttt tccccaccgt ggccgtcacg ctcagctcgg ccccggcgag cgcgatctgc
      901 tgcgcgcccg gcgtccccgg cggcacggcg cgcggctggc cgccctcctc gacaaacgga
      961 atgttcacca tcagcgaccg ccgcttgtca tcgagcccca gcgcggggac gacgcggccc
     1021 ggccggtcgt agacgcgcgg gatcaggtcc ttgagcacct tcccctcgta ggccatcgac
     1081 acgctggcga ggttgtcgag gtacccggaa ctgccgacct cggcctgccc ctgcagcgcg
     1141 cgaatcgcga cgccgctgcg ctcgtgcggg tcgagttgcc cgagcgacac gggcggcatg
     1201 ttcgtggtcc cgtgcaggtc gtccttcgcg gcggccgcgg cgagcgtcac ggcctgaatc
     1261 gcgggctcgg cgacgttgcg cgacggcggg ggggcctgcc cgccgctgta ggtcgtcagc
     1321 cgatacggca gatacggcag gttgcgcgtg ttggcctgct gccaccacgc ctcgtagcct
     1381 tcgagctgcc cgtcggcgat gagccactgc gcgcgcggcg ccagaccgac cgcctcgacc
     1441 tgcgccgagc gcatgtagtt gtaactttgc tgcgcgtcgc gcgcgaactg cacgatcccg
     1501 gtccagcgcc ggtcgccgtt gaggttcgcc tcgtcgccga tcaccggaat gatcgggatg
     1561 taggcgccat tccactcctg cggaccctgg aggatctcga cgccgttgat cagcgaccag
     1621 aagacgcggc ggccgcgcgt gaccgtccgg cgcggcagct tggcgccgcg gtcgcgctcg
     1681 acctgcgcca ggatcgtggc gggaatctcg gcctcgcgcg cggtcgtgtt gtccggtagc
     1741 agcacgagga cgtcggtctc ctcgcgcact tcccagtact cagccacgcg cacgcccacg
     1801 cccgcgtcgc cgtggctcgt cgtgatccat tgcgggatgt cgtcgccgag cgccgtcagt
     1861 tcgcggtcgg agtaggccgc gagcgtgctt gttgggtgtg cgcgtttgta gcgcgcgagc
     1921 ggcaggtcct gggtcaagag cgcgaactgg ccgtcgctcc aatcgggttc ctgcgcgaag
     1981 ggatcgagat acgcgctcgc ctgattcagc acgcgcttgt agatgatgcg ctgatcgaag
     2041 gtctgggtgt tgacgtactc ggtgaggatg cgatagacgc cgaagccgca tttggcggcg
     2101 cgctcgaacg cccattggcg cgcgaggtgc gcgcggctgt cggcctggat ggcgcgcgcg
     2161 atgtcgtcat acgcctgcgc gacgagttga ctcgcgccct cgccctcggg cgcgaacgac
     2221 aaccccagct tggcctgccg cgccgtgttg atgacttgct gcacggggcc gcgcagcaga
     2281 ttgaactcca ggcacggccg cgcggggacc gccggcaggc cgctcccggc gtcctgcccc
     2341 ccgcgcaggc gcttgatgtc gtcgtcccat tgcgcgccct tctcgtcgac gaagcgcagg
     2401 tcgtcgagct cgcgcgcgcg ctgatcgtgg acggcctcct cggacagccg gaagcggtcg
     2461 agggcggtct ggtgcgcggc cgggtcgggc gcgtcggggt cgatgggcgt ctcgcgtggg
     2521 acgacggcgg gcgggacggt ct
//
LOCUS       DASRDG010000073         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1046402, whole genome shotgun sequence.
ACCESSION   DASRDG010000073 DASRDG010000000
VERSION     DASRDG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046402"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..376
                     /locus_tag="VFA35_01210"
     CDS             11..376
                     /locus_tag="VFA35_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HZT54816.1"
                     /translation="MPAPIAWTRAVAVGSVVALIALGLAWELWLAPTGQRTLALKVVP
                     LLLPLPGLLRDRMYTYRWLSLMVWLYFTEGVVRATSDRGASAGLAVVELLLCLLLFAA
                     CAAHVRFRLRHANEAAACP"
     gene            367..1767
                     /locus_tag="VFA35_01215"
     CDS             367..1767
                     /locus_tag="VFA35_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZT54817.1"
                     /translation="MPLIAALRTAVGEANVVTDGDLSAWELDWRKRYRGRACAVVRPA
                     STPEVAAVVKACAAHGTAIVAQGGNTGLVGASVPDASGTQVLLSLARLNRVRAIDAAN
                     LTITVDAGCILQAVQEAAAAQGLLFALSLAAEGSCTIGGNLATNAGGTQVLRYGNARE
                     LCLGLEVVTAAGEVWDGLTGLRKDNTGYDLRDLFIGSEGTLGIITGATLKLHPQPAAV
                     TTALAALPTLAAAVELLQLARARLGAGLTGFEVMGCHALGLVRTHLPQLAQPLPPCAW
                     TVLLEQSDNESEAHARSLFEALLETALERGVIGDAAVAGSLEQSTAMWRLREAIPMAQ
                     ALEGANIKHDIALPVSAIPRFVAATDAALLRAFPGVRLVNFGHLGDGNLHYNVQAPDG
                     TDPAAFLREHERAVNTLVYDAVGAHAGSISAEHGVGALKRDELARRKSPVALQLMRAI
                     KQALDPNGLMNPGRVL"
     gene            complement(1785..2228)
                     /locus_tag="VFA35_01220"
     CDS             complement(1785..2228)
                     /locus_tag="VFA35_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HZT54818.1"
                     /translation="MKVLNLRCAHDHRFEGWFASDEDFSAQEQRGLIACPLCNDKAIA
                     RLPSAPRLNVSTQRGDASAPPGPAEQAHDRAQGRWLRAMRDMLGSTEDVGDRFPEEAR
                     RIHYGETPARGIRGHASAQDADALRDEGIEVVAVPMPGLLEGPLQ"
     gene            complement(2253..2846)
                     /locus_tag="VFA35_01225"
     CDS             complement(2253..2846)
                     /locus_tag="VFA35_01225"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZT54819.1"
                     /translation="MSAPQPLPDTHLREQTTRSEQVWRGRFLDVRRDQVRLPDGGAAQ
                     REYIVHPGAVMVVPLLDDGRLVVERQWRHPVGCVMLEFPAGKLDAGEPPLACGIRELM
                     EETGYRAAEWARAGILHNAIAYSNEGIEVWFARGLTPGERALDAGEFLDVTEMSAEQL
                     DELARRGELTDAKTLIGLLWLQNWRAGRWPLEWQPAP"
     gene            complement(2843..3142)
                     /locus_tag="VFA35_01230"
     CDS             complement(2843..3142)
                     /locus_tag="VFA35_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HZT54820.1"
                     /translation="MGSSAAVWLVLLIAVIAANLPFVNERLFVVGPSRAPKLIGWRLL
                     ELVVLWGATLAIGFALEARAGQVQPQGWEFYAALGFAFLTFAFPGFVWRYLRRGR"
BASE COUNT          463 a         1181 c         1187 g          449 t
ORIGIN      
        1 cgcacccgcg ctgcccgccc cgatcgcctg gacccgagcg gtcgcggtcg gcagcgtggt
       61 cgcgttgatc gcgctcggcc tcgcgtggga gctgtggctc gcgccgaccg gccagcgcac
      121 gctcgcgctg aaggtcgtgc cgctgctgct gccgctgccc gggctgctgc gcgaccgcat
      181 gtacacctac cgctggctga gcctgatggt gtggctctac ttcaccgagg gcgtggtgcg
      241 cgcgaccagc gaccgcggcg ccagcgccgg gctggccgtg gtcgagctgc tgctgtgcct
      301 gctgctgttc gccgcgtgcg cggcgcacgt gcgctttcgg ctgcgccacg cgaacgaggc
      361 ggcggcatgc ccctgattgc cgcgctgcgc acggccgtcg gcgaagcgaa cgtggtcacg
      421 gacggcgacc tgtcggcctg ggagctcgac tggcgcaagc gctatcgcgg ccgggcgtgc
      481 gcggtggtgc gcccggcgtc gacgcctgaa gtggcggccg tcgtgaaggc ctgcgccgcg
      541 catggcacgg ccatcgtcgc tcagggcggc aataccgggc tggtcggcgc ctcggttccg
      601 gacgcgagcg gcacgcaggt gctgctgagc ctggcgcgcc tgaaccgcgt gcgcgcgatc
      661 gatgcggcga acctcacgat cacggtcgac gccggctgca tcctgcaggc cgtgcaggag
      721 gccgcggccg cgcaaggcct gctgttcgcg ctgagcctcg cggccgaggg cagctgcacg
      781 atcggcggca acctcgcgac caacgccggc ggcacccagg tgctgcgcta cggcaacgcg
      841 cgcgagctgt gcctcggcct cgaagtggtg acagccgcgg gcgaggtctg ggacggcctc
      901 accggcttgc gcaaggacaa caccggctac gacctgcgcg acctgttcat cggcagcgag
      961 ggcacgctcg gcatcatcac cggcgcaacg ctgaagctgc acccgcagcc ggcggcggtg
     1021 acgaccgcgc tcgccgcgct gccgacgctg gccgccgcgg tcgagctgct gcagctcgcg
     1081 cgggcccggc tcggcgccgg cctgaccggc ttcgaggtga tgggctgcca cgcgctcggc
     1141 ctggtgcgca cgcacctgcc gcagctcgcg cagccgctgc cgccctgcgc gtggacggtg
     1201 ctgctggaac agtccgacaa cgagagcgaa gcacatgcgc gctcgctgtt cgaggcgctg
     1261 ctcgagacgg cgctcgaacg cggcgtgatc ggcgacgcgg cggtggccgg tagcctggag
     1321 cagtcgaccg cgatgtggcg cttgcgcgag gcgatcccga tggcgcaggc tctcgagggc
     1381 gcgaacatca agcacgacat cgcgctgccg gtgtcggcga tcccgcgctt cgtcgccgcc
     1441 accgatgccg cgctgctgcg cgcgttcccc ggcgtgcggc tcgtcaactt cggccacctc
     1501 ggcgacggca acctgcacta caacgtgcag gcacccgacg gcacggatcc ggcggcgttc
     1561 ctgcgcgaac acgaacgcgc ggtcaacacg ctcgtctacg acgcggtcgg cgcgcacgcc
     1621 ggctcgatct cagccgaaca cggcgtgggt gcgctcaagc gcgacgaact cgcgcggcgc
     1681 aagtcgccgg tcgcgctcca gctgatgcgc gcgatcaagc aggcgctcga tccgaacggc
     1741 ttgatgaacc cgggccgggt gctctgacga attcagtcgc cgcgctactg cagcgggcct
     1801 tcgagcagcc cgggcatggg caccgcgacg acctcgatgc cctcgtcgcg cagcgcgtcg
     1861 gcatcttggg ccgacgcatg gccgcgaatg ccgcgcgcgg gcgtctcgcc gtaatggatg
     1921 cggcgcgctt cctcgggaaa gcggtcgccg acatcctcgg tgctgcccag catgtcgcgc
     1981 atcgcgcgca gccaccggcc ttgcgcgcga tcgtgggcct gctcggccgg gccgggtggc
     2041 gcggacgcgt cgccgcgctg ggtcgagaca ttcagccgcg gcgcgctcgg cagccgcgcg
     2101 atggccttgt cgttgcacag cgggcaagca atcaggccgc gctgctcttg cgcggagaag
     2161 tcttcgtccg aggcgaacca gccctcgaag cgatggtcgt gcgcgcagcg caggttcaag
     2221 accttcatga ttcgattatc cggcgcgcac gctcacgggg ccggttgcca ttcgagcggc
     2281 cagcgcccgg cgcgccaatt ctgcaaccac agcaagccga tcagcgtctt ggcatcggtg
     2341 agttcgccac gtcgggcgag ctcgtcgagc tgttcggcgc tcatctcggt cacgtcgagg
     2401 aattcgccgg catcgagcgc gcgctcgccc ggcgtgagcc cgcgcgcgaa ccagacctcg
     2461 atgccttcgt tcgagtacgc gatcgcgttg tgcaggatgc cggcgcgcgc ccattcggcg
     2521 gcgcggtagc cagtctcttc catcagctcg cggatgccgc acgcgagcgg cggctcaccc
     2581 gcgtcgagct tgccggcggg gaattcgagc atcacgcacc cgaccgggtg gcgccactgg
     2641 cgctcgacga cgagccggcc gtcgtcgagc agcggcacga ccatcaccgc gccggggtgg
     2701 acgatgtact cgcgctgcgc ggcgccgcca tcgggcaggc ggacctggtc gcggcggacg
     2761 tcgagaaacc ggccgcgcca gacctgctcg gagcgcgtcg tctgctcgcg cagatgcgtg
     2821 tccggcaagg gctgcggcgc gctcatcggc cgcgcctcag gtagcgccag acgaagccgg
     2881 ggaacgcgaa ggtcaggaac gcgaagccga gcgcggcgta gaactcccag ccctgcggct
     2941 ggacctgccc ggcgcgcgcc tcgagcgcaa aaccgatcgc gagcgtggcg ccccagagca
     3001 cgaccagctc gagcagccgc cagccgatca gcttcggcgc gcggctcggg ccgacgacga
     3061 acagccgctc gttgacgaac ggcaggttgg ccgcgatcac cgcgatcagc agcaccagcc
     3121 agacggcggc agacgacccc atcgggcgag acgggtcagg tcgccagggc cttgacgatc
     3181 gcctgcacgc acagcgtcat caggccgctg ggcaggatgc cgaaggcgag caccgcgatg
     3241 ccgttcagcg agagcaccag gcgcacgtcg ccgctgctct
//
LOCUS       DASRDG010000074         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1392024, whole genome shotgun sequence.
ACCESSION   DASRDG010000074 DASRDG010000000
VERSION     DASRDG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392024"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFA35_01235"
     CDS             <1..543
                     /locus_tag="VFA35_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HZT54821.1"
                     /translation="NGALSAAITFTSASGDYNWSLSDGSSGTGTWTAGTPISLNGFAL
                     DLAGVPKSGDRIAVVPTVSVSANNGNALAFAKLGSAGIVAPAGGAGSGAAAQTLTDAY
                     AGALANIGVRVQGGKTAAGISGAVASQAESARANKAGVNLDEEAAKLIQFQQSYQAAA
                     KILQVAQAIFDTMLQAASSS"
     gene            552..1748
                     /gene="flgL"
                     /locus_tag="VFA35_01240"
     CDS             552..1748
                     /gene="flgL"
                     /locus_tag="VFA35_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HZT54822.1"
                     /translation="MRLSTFNTYSTALDTLVDRQARLAGTQEQMTTGKRVNRASDDPA
                     AAARAERALAAERRAVASQRAVDASNNAMTLTESALGDAGDLLQSAREAMVAAGNATY
                     GDAERNGIADQLQDLRDQLFAVANRSDGAGTYLFGGQGASQAPFVDQPGGVQFQGSSG
                     QLQSASGEALPLTVDGEATWMQARTGNGVFETRVVASAGAAVIDAGKVVDPAQLTGST
                     YRLQFSVGGGSTTYSVLKDGNPTAQTNLPFTPGQAIQVDGMSATITGAPANGDAFELA
                     PASSGLSVFDALDQAIADLRTPGRSNTQIAQGNAFALTRIDSVLGQLSSGRAQVGALM
                     NRVDAATERLSALKLSSQTERSNAEDLDMTQAISDFSNQQTGYDAALKAYSMVQRLSL
                     FNYINT"
     gene            1866..>2365
                     /locus_tag="VFA35_01245"
     CDS             1866..>2365
                     /locus_tag="VFA35_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZT54823.1"
                     /translation="MSDAAILGQVALGYSPFIDRNRSVTATRLTVFPLRPDAVLDVGQ
                     LLHEVGNVWPASGARVSLNVVSESLLQELLLARPSANLMVEVPAFMAADPANVDALVA
                     LHANGSTLLLKGRPLQELPREVLPCFKYSIIDLADDRRVTDPSPPPGVTRHIGHVQSG
                     VRSVAD"
BASE COUNT          372 a          868 c          822 g          303 t
ORIGIN      
        1 aacggcgcgc tgagcgccgc catcaccttc acctcggcaa gcggcgacta caactggtcg
       61 ctcagcgacg gcagcagcgg caccggcacc tggaccgcgg gcacgccgat cagcctgaac
      121 ggcttcgcgc tcgacctcgc cggcgtgccc aagagcggcg accggatcgc cgtcgtgccg
      181 accgtttcgg tcagcgcgaa caacggcaac gcgctcgcgt tcgcgaagct cggcagcgcc
      241 ggcatcgtgg cgcccgccgg cggcgcgggc tccggcgccg cggcgcagac cctcaccgac
      301 gcctatgccg gcgcgctcgc caacatcggc gtgcgcgtgc agggcggcaa gacggcggcg
      361 ggcatctccg gcgcggtcgc gagccaggcc gagtcggcgc gcgccaacaa ggccggcgtg
      421 aacctcgacg aagaggcggc gaagctgatc cagttccagc agagctacca ggcggccgcg
      481 aagatcctgc aggtcgcgca agccattttc gacacgatgc tgcaagcggc gtcttcttcc
      541 tgaggccggc gatgcgcctg agcaccttca acacttacag caccgcgctc gacacgcttg
      601 tcgaccgcca ggcccggctc gccggcacgc aggagcagat gacgaccggc aagcgcgtca
      661 accgcgccag cgacgacccg gccgctgccg cgcgcgccga gcgcgcgctc gccgccgagc
      721 gccgcgcggt cgcgagccag cgcgccgtcg atgccagcaa caacgcgatg acgctgaccg
      781 agagcgcgct cggcgatgcc ggcgacctgc tgcagtcggc gcgcgaggcg atggtcgcgg
      841 ccggcaacgc gacctacggc gacgccgagc gcaacggcat cgccgaccag ctgcaggacc
      901 tgcgcgacca gttgttcgcg gtcgcgaacc gcagcgacgg cgccggcacc tacctgttcg
      961 gcggccaggg cgcgagccag gcgccgttcg tcgaccagcc cggcggcgtg cagttccagg
     1021 ggagctcggg ccagctgcaa tcggcctccg gcgaagcgct gccgctgacc gtggacggcg
     1081 aggccacctg gatgcaggcg cgcaccggca acggcgtgtt cgagacgcgc gtggtcgcca
     1141 gcgccggcgc tgcggtgatc gacgccggca aggtggtcga ccccgcgcag ctgaccggct
     1201 cgacctaccg cctgcagttc agcgtcggcg gcggcagcac gacctactcg gtcctgaagg
     1261 acggcaaccc gaccgcgcag acgaacctgc cgttcacgcc cggccaggcg atccaggtcg
     1321 acggcatgtc ggcgacgatc accggcgccc ccgcgaacgg cgatgcgttc gagctcgcgc
     1381 cggccagctc cggcctgagc gtgttcgacg cgctcgacca ggcgatcgcc gatctgcgca
     1441 cgcccggccg cagcaacacg cagatcgcgc agggcaacgc gttcgcgctg acccggatcg
     1501 attcggtgct cggccagctg agttcggggc gcgcccaggt cggcgcgctg atgaaccggg
     1561 tcgacgcggc gaccgagcgt ttgtcggcgc tgaagctctc gagccagacc gagcgctcga
     1621 acgccgaaga cctcgacatg acgcaggcga tttccgattt ctcgaaccag cagaccggct
     1681 acgatgccgc cctcaaggcg tactcgatgg tgcagcgcct gtcgctgttc aactacatca
     1741 atacctgaca tgaccttcag cgcgaacgcg cagattccag ctgcgggccg agcgccgggc
     1801 cgccccaagc cggcccgcca tcccctcggg ggatcggccg acgtactcgt tggacgagcg
     1861 gcgccatgag cgatgcggcc attctgggcc aggtggcctt gggctattcg cccttcatcg
     1921 accgcaaccg cagcgtcacc gcgacccggc tgacggtgtt cccgttgcgg cccgatgcgg
     1981 tgctcgatgt cgggcagctg ctgcacgagg tcggtaacgt ctggccggcg agcggcgcgc
     2041 gggtgtcgct caacgtcgtc agcgagagcc tgctgcagga gctgctgctc gcgcggccgt
     2101 cggcgaacct gatggtcgag gtgccggcct tcatggcggc cgacccggcc aacgtcgacg
     2161 cgctggtcgc gttgcacgcc aacggcagca cgctgctgct gaagggccgc ccgctgcagg
     2221 agctgccgcg cgaggtgctg ccgtgcttca agtactcgat catcgatctc gccgacgacc
     2281 ggcgcgtgac cgatccgagc ccgccgccgg gcgtgacacg ccacatcggc cacgtgcagt
     2341 cgggcgtgcg cagcgtcgcc gacat
//
LOCUS       DASRDG010000075         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1518428, whole genome shotgun sequence.
ACCESSION   DASRDG010000075 DASRDG010000000
VERSION     DASRDG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518428"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..700
                     /locus_tag="VFA35_01250"
     CDS             77..700
                     /locus_tag="VFA35_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZT54824.1"
                     /translation="MKIGVIGSGRIGSTIGGLWVKAGHEVMFSDRDPEAVKRAIADLG
                     PRAKAGSVHEAASFGDAVLIAVPYAALPAIRDEVGLRLRGKVVIDPNNPVPARDGDMA
                     VAAKEKGAAVSTAALLPGVKTVRSFNSWGYATMAREANRPAPRMALPVAADDAEAMKV
                     GMQLVSDAGFDPVNAGSLASSKAYDLGSAVSGKVMTADELRKALSIR"
     gene            697..1941
                     /locus_tag="VFA35_01255"
     CDS             697..1941
                     /locus_tag="VFA35_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT54825.1"
                     /translation="MKWLTRVVAVRPEEVRALLWSFAYFFCLLSGYYILRPLRDEMGI
                     AGGVRNLQWLFTATFVVMLAAVPIYGALVARVRRRVFIPLVYHFFVANLAIFWLLLTF
                     DVERQTVARVFFVWISVFVLFAVSVFWSFMADLYSSEQGKRLYGFIAAGGSVGALAGP
                     ALTIGLSHVIGAVNLLIVAALLLEVAVFCARKLEVRKDVASETQAPIGGGAIDGLIMV
                     LRSPYIAGIMLWVALMSVAATFLYFEQAAIVAAASDDPAVRTRIFAAVDLAVGLLTLV
                     VQFAATGRLIERFGVGPSLALLPFVFAAGFAVLAAYPVLAVVIAFQALQRTANFAISN
                     PAREVCFTVLARDEKYKAKNVIDIVAVRGADAAGGWLFTGLRTLGMEVRSIPLVAIAI
                     AAVTLVLAMALGKALERRSKPA"
BASE COUNT          253 a          693 c          707 g          372 t
ORIGIN      
        1 cgctatgaaa agacgcgcgt tcctctccgc agtactggcg gccgcggtgg ttccggcctt
       61 cgcacaaacc aagccgatga agatcggcgt catcggctcg ggtcgcatcg gctcgaccat
      121 cggcggcctg tgggtgaagg cgggccacga ggtgatgttc tccgaccgcg atcccgaagc
      181 ggtgaagcgc gccatcgcgg acctgggacc gcgcgcgaaa gcgggcagcg tgcacgaggc
      241 tgcgtcgttc ggcgatgcgg tgctcatcgc ggtgccttac gcggcgctgc cggcgatccg
      301 cgacgaggtc ggcctgcgcc tgcgcggcaa ggtggtgatc gacccgaata atccggtccc
      361 ggcgcgcgac ggcgacatgg ccgtcgccgc gaaggagaaa ggcgctgcgg tctcgaccgc
      421 ggcgctgctg cccggcgtga agaccgtgcg cagcttcaat tcctggggct acgcgacgat
      481 ggcgcgcgag gcgaaccgtc cggcgccgcg catggcgctg ccggtggcgg ccgacgatgc
      541 cgaggcgatg aaggtcggca tgcagctcgt ctccgacgcg ggcttcgacc cggtcaacgc
      601 cggctcgctc gcctcgtcga aggcctatga cctcggctcc gcggtctccg gcaaggtgat
      661 gaccgccgac gagctgcgca aggcgctctc gatccggtga agtggctcac ccgggtcgtc
      721 gcggtacgac ccgaggaagt gcgggcgctg ctctggtcgt tcgcctattt cttctgcctg
      781 ctttccgggt attacatcct gcggccactg cgcgacgaga tgggcattgc cggcggcgtg
      841 cgcaacctgc agtggctctt caccgccact ttcgtcgtga tgctcgccgc ggtgccgatc
      901 tacggcgcgc tggtggcgcg cgtgcggcgg cgggtcttca tcccgctggt ctatcacttc
      961 ttcgtcgcca acctcgcgat cttctggctg ctgctcacct tcgacgtcga gcgccagacg
     1021 gtggcgcgcg tgttcttcgt ctggatcagc gtcttcgtgc tgttcgcggt gtcggtgttc
     1081 tggtcgttca tggccgacct gtacagctcc gagcagggca agcgcctcta cggcttcatc
     1141 gccgcgggcg gcagcgtcgg cgcgctcgcc gggccggcgc tgacgatcgg cctgtcgcac
     1201 gtcatcggcg cggtgaatct cctgatcgtc gcggcgctgc tgctcgaggt ggccgtgttc
     1261 tgcgccagga agctcgaagt cagaaaagat gttgcttcgg aaacccaggc gccgatcggc
     1321 ggcggggcga tcgacgggct gatcatggtg ctgcgctcgc cctacatcgc cggcatcatg
     1381 ctctgggtgg cgctgatgtc ggtcgccgcg accttcctct atttcgagca ggcggcgatc
     1441 gtggccgccg cttccgacga cccggcggtg cgaacgcgca tcttcgccgc cgtcgatctc
     1501 gccgtgggcc tcttgaccct cgtcgtgcag ttcgccgcca ccggccggct gatcgagcgc
     1561 ttcggcgtcg gcccgtcgct cgccctcctg ccgttcgtct ttgccgcggg cttcgcggtg
     1621 ctcgccgcct atccggtgct ggcggtggtg atcgccttcc aggcgctgca gcgcaccgcc
     1681 aacttcgcca tctccaaccc ggcgcgcgag gtctgcttca cggtgctcgc gcgcgacgag
     1741 aagtacaagg cgaagaacgt gatcgacatc gtcgcggtgc gcggcgcgga tgccgcgggc
     1801 ggctggctct tcaccgggct gcgcacgctc ggcatggagg tgcgctcgat cccgctggtc
     1861 gcgatcgcga tcgccgccgt gaccctcgtc ctggcgatgg cgctcggcaa ggcgctggag
     1921 cgccgcagca agcccgccta gcggttcagc ttcttcagga tctcgctgcg ggtctggccg
     1981 accgcgtcgg cgtgcgatcc gcccacggcc gccgcgcgat cgatg
//
LOCUS       DASRDG010000076         5776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1046619, whole genome shotgun sequence.
ACCESSION   DASRDG010000076 DASRDG010000000
VERSION     DASRDG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046619"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1380
                     /locus_tag="VFA35_01260"
     CDS             322..1380
                     /locus_tag="VFA35_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54826.1"
                     /translation="MVAGLLAATAPLLGGCTTTGVLLSVAGVATDTSITWEIVKHLHA
                     KLIEGGDMPCHRLDSVERALNVRCGAFVPGSLRVAEMQASKLQGCALTVAVRDPRFWP
                     ALPEFIAKGASPEGCARSPLVELAQASSFPEGCPDFSTASPAVLQSMRWLAEADARAI
                     HHDVVRMLSCPSAVRAGLDAPLATWLAQGDLDVGKVAFGPLGALHPDYLDTPFARALE
                     AQGHTARAGLGSFDGVQPRGFELALRNSQWAALDWWITRVPELANRVPPVQGNQLPWV
                     PLARVLTPNFLTDPASQTEMVEFLLAHGANPWQKLPFDAGSSVVQYARVLKSPQLALL
                     DPPVVPTVVAATAGGVAR"
     gene            complement(1390..3168)
                     /locus_tag="VFA35_01265"
     CDS             complement(1390..3168)
                     /locus_tag="VFA35_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HZT54827.1"
                     /translation="MTAPADPKPSSNFLRQIVERDLAEGTYAQRRFAGTPGDAAHHAA
                     GQPDPAKIRTRFPPEPNGYLHIGHAKSITLNFGLAAEYAGVCHMRFDDTNPEKEEQEY
                     VDSILDAVKWLGFDWHAHGTSHLYYASNYFDFMYRAAEALIEAGLAYVDEQSAEEMRA
                     NRGDFGHAGTDSPFRNRTPAENLARFRAMRAGELPDGAAVLRAKLDMASPNINLRDPA
                     LYRIKHAEHHNTGDTWCIYPMYTYAHPIEDALEGITHSICTLEFEDQRPWYDWLLDTL
                     CRLGLLNAPRPHQYEFARLNVTYVVTSKRKLRQLVDEGIVDGWDDPRMPTIAGLRRRG
                     YTPEALRLMCERAGTSKAGGWTDYASLDIALREDLEGKAPRAMAVIDPVKLKLTNWAE
                     LFGSADHREPCSAPANPHRPELAPRAFSLGNEDWIERDDFAEVPAKGFFRLFPGNRVR
                     LKYGYVVECTGCEKDANGAVTAVTATVVPDTKSGTPGADAVKVKGTITWLGVHDALPA
                     ELRLYDRLFTEAQPDAGGKDFKAALNPASKAVVASYVEPSLANAQPDAKFQFERHGYF
                     VADRVDHTPGQPVFNRIAGLKDSWAK"
     gene            3297..3626
                     /locus_tag="VFA35_01270"
     CDS             3297..3626
                     /locus_tag="VFA35_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54828.1"
                     /translation="MIKSLVVAAVATVGVLGATGAQAGVSWSVNVNTPLVGTVVSGGP
                     GYYAPRYAGYCDPGYYAYAPAPVYRVAPPVAYYPAPVYYRPVPVVVYPRYQGGWRHGH
                     GRWHGHR"
     gene            3736..4206
                     /locus_tag="VFA35_01275"
     CDS             3736..4206
                     /locus_tag="VFA35_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HZT54829.1"
                     /translation="MFIHRFSTLAAVSMLAVLAGCVVAPPRERVVYAPQPAQRQVPVY
                     AEYGRVENIGYVQVAQRPSGAGAVLGAVIGGVVGNRFGEGAGRALATGVGAVGGAVVG
                     NAIENQNRNLRDGEVYRVQVRFDNGTVRDFDFQRIDDLRIGDRVKFDGGQLHRL"
     gene            complement(4247..5075)
                     /locus_tag="VFA35_01280"
                     /pseudo
     CDS             complement(4247..5075)
                     /locus_tag="VFA35_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
     gene            complement(5072..>5776)
                     /locus_tag="VFA35_01285"
     CDS             complement(5072..>5776)
                     /locus_tag="VFA35_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZT54830.1"
                     /translation="LVPATVITYWFYSRETIAAKEAAMQAAQRQAAEHQLKLLESQLE
                     PHMLFNTLANLRVLIGVDPQRARAMLDQLIAFLRATLAGSRAAQHPLRAEFARLTDYL
                     ALMQVRMGGRLQTRVELPEALAELPVPPLLLQPLVENCIKHGLEPALAGGCIDVRAAR
                     DGGTLVLSVRDTGAGLSTPAVEGTQFGLVQVRERLAALYGARAALTLTRADDAEGGTL
                     ATIRLPIVPATEPAAP"
BASE COUNT          768 a         1972 c         2103 g          933 t
ORIGIN      
        1 gtcagcgcgg cagaaagaag gagggcgagc ggcagcgtcg cgcgggcaga aagtcgtttc
       61 atcgggaagg cgggtgatgg ggatcggcca agcgcacgca tcatgccgca cgcggcgaca
      121 cacgaagtta cgcgtcggcc gaatgtcggc ccatctcgac gcatcgcgcg gcatccggtc
      181 gaccaggatc gtcgttccgc cgcggttgcc gcgccgatct ttgccggtgc ggaaattgca
      241 accagtgatt cggcgcgggc ggcgacacgg agcagggcga tgcaggcaat gcgagcgact
      301 cgatctggat tctcgaagtg gatggttgcc ggcctgctcg ccgccaccgc accgctgctc
      361 ggcggctgca ccacgaccgg cgtgctgctc agcgtcgccg gcgtcgcgac cgacaccagc
      421 atcacctggg agatcgtcaa gcacctgcac gccaagctga tcgaaggcgg cgacatgccc
      481 tgtcatcgcc tcgacagcgt cgagcgcgcg ctcaacgtgc gttgcggcgc cttcgtgccc
      541 ggcagccttc gcgtggcgga gatgcaagcc tcgaagctgc agggctgcgc gctgaccgtc
      601 gcggtgcgcg acccacgctt ctggccggcg ctgccggagt tcatcgcgaa aggcgcatcg
      661 cccgagggct gcgcgcgttc gccgctggtc gagctcgcac aagcgtcgtc atttcccgag
      721 ggctgccctg atttctcgac cgcgtcaccc gccgtgctgc aatcgatgcg ctggctggcc
      781 gaggccgacg cgcgcgcgat ccaccacgac gtcgtgcgca tgctcagctg cccgagtgcg
      841 gtgcgtgccg ggctcgacgc gccgctcgcc acctggctcg cgcaaggcga tctcgatgtc
      901 ggcaaggtcg cgttcggccc gctcggcgcg ttgcatcccg actacctcga cacgccgttc
      961 gcgcgcgcgc tcgaagccca gggccacacc gcgcgcgcgg gcctcggcag cttcgacggc
     1021 gtgcagccgc gcggcttcga gctcgcactg cgcaacagcc agtgggccgc gctcgactgg
     1081 tggatcacgc gcgtgcccga gctcgcgaac cgcgtcccgc cggtgcaggg caaccagctg
     1141 ccgtgggtgc cgctcgcgcg cgtgctgacg ccgaacttcc tgaccgaccc ggcgagccag
     1201 accgagatgg tcgagttcct gctcgcgcat ggcgcgaacc cgtggcagaa gctgccgttc
     1261 gatgccggca gctcggtcgt gcagtacgcg cgcgtgctga agagcccgca gctcgcgctg
     1321 ctcgacccac ccgtcgtgcc gacggtggtc gccgcgaccg cgggcggagt cgcccggtag
     1381 cgcgcggggc tacttcgccc aggagtcctt caggcccgcg atccggttga acaccggctg
     1441 gcccggcgtg tgatcgacgc ggtcggcgac gaagtagccg tggcgctcga actggaactt
     1501 ggcgtcgggc tgcgcgttcg ccagcgaggg ctcgacgtag ctggcgacga cggccttgct
     1561 ggccgggttc agcgcggcct tgaagtcctt gcccccggcg tcgggctggg cctcggtgaa
     1621 caggcggtcg tacaggcgca actcggccgg cagcgcgtcg tgtacgccga gccaggtgat
     1681 cgtgcccttg accttgaccg catcggcgcc gggcgtgccg ctcttggtgt cgggcacgac
     1741 ggtcgcggtg accgcggtga ccgcgccgtt cgcgtccttc tcgcagccgg tgcactcgac
     1801 cacgtagccg tacttcagcc gcacccggtt gcccgggaac agccggaaga agcccttcgc
     1861 cggaacctcg gcgaagtcgt cgcgctcgat ccagtcctcg ttgccgaggc tgaacgcgcg
     1921 cggcgcaagt tcggggcggt gcgggttcgc gggcgcgctg cagggctcgc ggtggtcggc
     1981 gctgccgaac agctcggccc agttcgtgag cttcagcttc accgggtcga tcaccgccat
     2041 cgcgcgcggc gccttgcctt cgaggtcttc gcgcagcgcg atgtccaggc tcgcgtagtc
     2101 ggtccagccg cccgccttgc tggtgccggc gcgctcgcac atcaggcgca gcgcctcggg
     2161 tgtgtagccg cgccggcgca ggccggcgat cgtgggcatg cgcgggtcgt cccagccgtc
     2221 gacgatgccc tcgtcgacga gctgccgcag cttgcgcttg ctcgtgacca cgtaggtcac
     2281 gttcaggcgc gcgaactcgt actggtgcgg gcgcggggcg ttcagcaggc cgaggcggca
     2341 cagcgtgtcg agcagccagt cgtaccacgg gcgctggtct tcgaattcga gcgtgcagat
     2401 gctgtgggtg atgccttcga gcgcgtcctc gatcgggtgc gcgtaggtgt acatcgggta
     2461 gatgcaccaa gtgtcgccgg tgttgtggtg ctcggcgtgc ttgatgcggt agagcgccgg
     2521 gtcgcgcagg ttgatgttcg ggctcgccat gtcgagcttg gcgcgcagca cggccgcgcc
     2581 gtcgggcagc tcgcccgcac gcatcgcgcg gaagcgcgcg aggttctcgg ccggagtgcg
     2641 gttgcggaac gggctgtcgg tgcccgcatg gccgaagtcg ccgcggttcg cgcgcatctc
     2701 ttcggcgctc tgctcgtcga cataggcgag gccggcttcg atcagcgcct cggccgcgcg
     2761 gtacatgaag tcgaagtagt tgctggcgta gtagaggtgg ctcgtgccgt gggcatgcca
     2821 gtcgaagccg agccacttga cggcgtcgag gatgctgtcg acgtactcct gctcttcctt
     2881 ctcgggattg gtgtcgtcga agcgcatgtg gcacacaccc gcgtactcgg cggcgaggcc
     2941 gaagttcagc gtgatgctct tcgcgtggcc gatgtgcagg tagccgttcg gctcgggcgg
     3001 aaagcgggtg cggatcttcg ccgggtcggg ctggccggcg gcgtggtgcg cggcatcgcc
     3061 cggcgtgccc gcgaagcgcc gctgcgcgta ggtgccctcg gccaggtcgc gctcgacgat
     3121 ctggcgcagg aagttgctgc tgggcttggg gtcggcgggt gcggtcatgg gcggcgattt
     3181 tgccagcaac gtccatttac gcttgctcac ccgaatgctg tcagccggcg gaaccccggt
     3241 gcacgttgaa gttctcatcc aggtcgccca ttcgggccct gcagcaggag agacagatga
     3301 tcaaatcgtt ggttgtcgcc gccgtcgcca cggtcggcgt gctcggtgcc actggcgccc
     3361 aggccggcgt gtcgtggtcg gtcaacgtca acaccccgct ggtcggtacc gtcgtctcgg
     3421 gcgggcccgg gtactacgcg ccgcggtatg cggggtattg cgacccgggc tattacgcct
     3481 atgcgcctgc gccggtctac cgcgtggcgc cgccggtggc ctactacccg gcgccggtgt
     3541 actaccggcc cgtgcccgtg gtggtctacc cgcgctacca gggtggctgg cgccatggcc
     3601 acggccgttg gcacggccat cgttgaccgc gctgccccgc gatggacacg gtgagctcca
     3661 agtaaaagaa tgcgtctgcc cgctggctgc ggcgatgctg cgctgaccgc gatctcgacc
     3721 cgaaggagtt ctgccatgtt tatccatcgc ttttcgaccc tcgccgccgt gtcgatgctc
     3781 gccgtgctgg ccggttgcgt cgtcgcgccg ccgcgtgagc gagtcgttta cgcacctcag
     3841 ccggcgcagc gccaggtgcc ggtctacgcc gaatacggcc gcgtcgagaa catcggctac
     3901 gtgcaggtcg cgcagcgccc gagcggcgcc ggcgcggtgc tcggtgcggt gatcggcggc
     3961 gtcgtcggga accgcttcgg cgaaggtgcg ggccgggcgc ttgcgaccgg cgtgggggcg
     4021 gtcggcggtg cggtcgtcgg caacgcgatc gagaatcaga accgaaactt gcgcgacggc
     4081 gaggtctatc gcgtgcaggt gcgtttcgac aacggcacgg ttcgcgactt cgacttccag
     4141 cgcatcgacg acctgcgcat cggcgaccgc gtgaagttcg acggcgggca actgcaccgc
     4201 ctctgactcc gcgccaacgc gctgcgtcgc gcggctcggt tgcgcgttac atcgccttga
     4261 acaggtgtgc gtagagccgg ctcgcgacga gcttgtcggg gtggccgcgc agcgtgatgt
     4321 acaccttgcc cgactcgtcg cgcgccgcgg tcgcaatcgc gtccgcgcgc acgacggtgc
     4381 cgcggtggat ctgccagaag cattggccgt cgagttgcgg caacagctcg cgcagcgcgg
     4441 tgcggatcag gtactggcgc gcggcggtga cgacgcgcac gtacttgtcg gctgactcga
     4501 agtacagcac ctcggcgacc ggcaccatgt ggatcgcggc gccgacactc gcctggatca
     4561 cgccgagccg ctgcggccgc cgcacccgtt gcggccgcga ggcgcggtgc ggcgagcaac
     4621 tggcgcagtt gcgtcagcgc ggcatcgagt gcgggcgcag tgggcaccgc actcgccgca
     4681 ggcgtgcggc gcgcctgcag cgcggcctgc agccgcgcgc aggtctgcgc gaggcgatcg
     4741 gcctgcaccg gcttcagcac gtagtcgatc gcggcgcgct cgaaggcctg cagcgcgtac
     4801 tggtcgtagg cggtgacgaa cacgagcagc ggcagcggcg tgccctcggg ccagtcttcg
     4861 gccagcgcct gcgcggcttc cagcccgctc atgccgggca tgcggatgtc gaggaacacc
     4921 acgtcgggct gcagcgccag cccctgctcg accgccgcgg cgccgtggcc gacgttggcg
     4981 acgatctgca gctcgggcca gcgctgcgcg agctcggctt gcaggttcgc ggcgagcagc
     5041 ggctcgtctt cggcaatcag cgccgtggcg ttcacggcgc ggcgggctcg gtggccggca
     5101 cgatcggcaa tcggatcgtc gcgagcgtgc cgccctcggc atcgtccgcg cgtgtcagcg
     5161 tcagcgcggc gcgcgcgccg tacagcgcgg ccagccgctc gcgcacctgc acgaggccga
     5221 actgcgtgcc ttcgacagcc ggcgtcgaca ggccggcgcc ggtgtcgcgc acgctgagca
     5281 cgagcgtgcc gccgtcgcgc gccgcgcgca cgtcgatgca cccgcccgcc agcgccggct
     5341 ccaggccgtg cttgatgcag ttctcgacca gcggctgcag cagcagcggc ggcaccggca
     5401 gctcggccag cgcttcgggc agctcgaccc ttgtctgcag ccgcccaccc atgcggacct
     5461 gcatcagcgc gaggtagtcg gtcagccgcg cgaactcggc gcgcagcggg tgctgcgcgg
     5521 cgcgcgagcc ggccagcgtg gcacgcaaga aggcgatcag ctggtcgagc atcgcccgcg
     5581 cgcgctgcgg gtcgacgccg atcagcacgc gcaggttcgc cagcgtgttg aacagcatgt
     5641 gcggctcgag ctgtgattcg agcagcttca gctggtgctc ggcggcctgg cgctgcgcgg
     5701 cctgcatcgc ggcctctttc gcggcgatgg tttcgcgcga atagaaccag taggtgatca
     5761 cggtggccgg cacgag
//
LOCUS       DASRDG010000077         5053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1518496, whole genome shotgun sequence.
ACCESSION   DASRDG010000077 DASRDG010000000
VERSION     DASRDG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518496"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /gene="thrH"
                     /locus_tag="VFA35_01290"
     CDS             <1..359
                     /gene="thrH"
                     /locus_tag="VFA35_01290"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HZT54831.1"
                     /translation="IILSDTFYEFAQPLMQQLAWPTLMCHQLEVDAEGFVRRHVLRQP
                     DQKRHAVNALRGLNFQVIAAGDSYNDTGMLGAAHAGFFIHAPEAIVKQFPQFPVTHGY
                     TELTTAIDAAVARLRG"
     gene            complement(367..1110)
                     /locus_tag="VFA35_01295"
     CDS             complement(367..1110)
                     /locus_tag="VFA35_01295"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HZT54832.1"
                     /translation="MTETTPASAPARQRFDRLDALRALAIVWMAVFHFCFDLNHFGFI
                     RQDFYRDPAWTLQRTCIVSLFLFCAGVGQAVALDQRQPWPRFRRRWAQVAGCAVLVTL
                     GSVWMFPRSFISFGVLHCIAVALIVMRLSGGAGRWLWPLGLVAVLLPQFVQHPFFDTR
                     WTNWLGLVMHKPVTEDYVPLLPWLGVMWWGLAAGQWVLAERRAWLTGALPPVLGPLAT
                     LGRWSLSFYMLHQPVLIGLLSAIAALRSA"
     gene            complement(1115..1531)
                     /locus_tag="VFA35_01300"
     CDS             complement(1115..1531)
                     /locus_tag="VFA35_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZT54833.1"
                     /translation="MPFVLDNSIVCGWFLENQATPYADVIIERLRDDRAVVPALWELE
                     FTNVLRTACLRQRMTAEAAQRVIAQIVSLPIDVDRQAVPASEILGLALRFGLSSYDAA
                     YLELALRLQLPIATGDGPLRAAALASGVGTATATVG"
     gene            complement(1535..1789)
                     /locus_tag="VFA35_01305"
     CDS             complement(1535..1789)
                     /locus_tag="VFA35_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZT54834.1"
                     /translation="MKEIAVYEAKTRLSELLAEVEQGEQVTITRRGLPIARLVSAVAA
                     RRGAPSQRQQVAATIASLREQRKGVVLEGDLRDLIADGRD"
     gene            complement(1835..>5053)
                     /gene="purL"
                     /locus_tag="VFA35_01310"
                     /gene_synonym="purI"
     CDS             complement(1835..>5053)
                     /gene="purL"
                     /locus_tag="VFA35_01310"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HZT54835.1"
                     /translation="PQHTIVAYSDNASVMEGGTIERWLPQGYTNAPVYAARTEPVHVL
                     MKVETHNHPTAISPFPGAATGAGGEIRDEGATGRGSKPKAGITGFSVSNLHLPGTNEP
                     WEQAPIGKPEHIASPLQIMIDGPLGGAAFNNEFGRPNLGGYFRVYEQDVAGQRRGYHK
                     PIMIAGGLGSIAASQTKKIAFPPGTLLIQLGGPGMRIGMGGGAASSMAAGANAAALDF
                     DSVQRGNPEIERRAQEVINHCWALGESNPILAIHDVGAGGISNAFPELVDGAGVGARF
                     DLRQVPLEETGLAPKEIWCNESQERYVMAVAPEALPIFRAMAERERCPFAVVGVATAA
                     KELILEDGPGGEQSIAMPMDVLLGKPPKMLRDVQRVAREAPKLDLNDVTLQQVACDVL
                     RHPTVASKRFLITIGDRTVGGLSSRDPMVGPWQVPVADCAVTLADFSGFRGEAMAIGE
                     RTPLAALDAPASGRMAVGEAITNLLAAPIELARVKLSCNWMAACGEPGEDAALYDTVA
                     AVGMELCPALGIGVPVGKDSLSMRTRWRDGDEAKQVTAPVSLIVTAFATLDDVRATLT
                     PQLQPGDTTLILIDLGAGRNRLAGSMLAQVLNQFGSLADDGVPDLDDPAQLKALVAAI
                     NQLRAQGRLLAYHDRSDGGLWAAACEMAFAGHLGVSLNIDMLVTEGDGISDSRAEYGD
                     SKNWAAQVGARRHELTLRALFSEELGAVIQVPTAVRNEVMQLLREHGLSKHSHFIGKP
                     NERGVMEVWRDAKAVFSAPLRDLHQAWDEVSWRIAKLRDNPAGADAEHEAAGRVDDPG
                     LHVALSFDPAVDVAAPFIGKARPKLAILREQGVNSHLEMSYAMDRAGFETFDVHMSDL
                     QAGRARLDQFQGFVACGGFSYGDTLGAGEGWARSVMFNPALAEQFARFFNRGDTFALG
                     VCNGCQMMAALAPIIPGASAWPKFTRNRSEQFEARLSLVEVLDSPSIFFTGMAGSRLP
                     VAVAHGEGFADFSQRGDPKAVHRAMRFVDNAGNPTEAYPFNPNGSPGGLTAVTTADGR
                     FTALMPHAERVFRNLQMSWTSGERSAASPWTRMFANARVWVN"
BASE COUNT          753 a         1813 c         1743 g          744 t
ORIGIN      
        1 tgatcatcct ctcggacacc ttctacgagt tcgcgcagcc gctgatgcag cagctcgcgt
       61 ggccgacgct gatgtgccac cagctcgagg tcgatgccga aggcttcgtg cgccgtcacg
      121 tgctgcgcca gcccgaccag aagcgccacg cggtcaacgc gctgcgcggc ctgaacttcc
      181 aggtgatcgc cgcgggcgac tcgtacaacg acaccggcat gctcggtgcc gcgcatgccg
      241 gcttcttcat ccacgcgccg gaggcgatcg tcaagcagtt cccgcagttc ccggtgacgc
      301 acggctacac cgaactgacg accgcgatcg acgcggcggt cgcgcggctg cgcggctgat
      361 cgcccctcag gcggagcgca gtgccgcgat cgcgctcagc agcccgatca gcaccggctg
      421 gtgcagcatg taaaagctca ggctccagcg gccgagcgtg gccagcgggc cgagcaccgg
      481 cggcagcgcg ccggtgagcc aggcgcggcg ctcggccagc acccattggc cggccgccag
      541 gccccaccac atcacgccca accacggcag cagcggcacg tagtcttcgg tgaccggctt
      601 gtgcatgaca aggccgagcc agttggtcca gcgggtgtcg aagaacgggt gctggacgaa
      661 ctgcggcagc agaaccgcca ccagacccag cggccacaac cagcgcccgg cgccacccga
      721 gagccgcatc acgatcagcg cgaccgcgat gcagtgcagc acgccgaaac tgatgaagct
      781 gcgcgggaac atccagaccg agccgagcgt gacgagcacc gcgcagcccg ccacctgcgc
      841 ccagcggcgc cggaagcgcg gccagggctg gcgctggtcg agcgcgaccg cctggccgac
      901 gccggcgcag aacaggaaca ggctgacgat gcaggtgcgc tgcagcgtcc acgccggatc
      961 gcgatagaag tcctgccgga tgaagccgaa gtggttcagg tcgaaacaga agtggaagac
     1021 cgccatccag acgatggcga gcgcgcgcag cgcatcgagg cggtcgaagc gctggcgcgc
     1081 gggggcggat gcgggggtcg tctcggtcac gcggctaacc gacggtcgcg gtcgccgtgc
     1141 cgacgcccga agccagcgcg gcggcgcgca aggggccgtc gccggtggcg atcggaagct
     1201 ggagccgcaa tgcgagttcg aggtaggccg cgtcgtagct gctcaggccg aagcgcagcg
     1261 ccaggccgag gatctcgctc gccggaaccg cctgccgatc cacgtcgatc ggcaaggaca
     1321 cgatctgcgc gatcacgcgc tgcgccgcct cggccgtcat gcgctggcgc aggcaggccg
     1381 ttcgcagcac gttggtgaat tccagctccc acagcgccgg caccacggca cgatcgtcgc
     1441 gcagacgctc gatgatgacg tcggcatacg gcgtcgcctg gttctcgaga aaccagccgc
     1501 agacgatgga attgtcgaga acgaagggca caggtcagtc gcggccgtcg gcgatcagat
     1561 cgcgcagatc gccttcgagc acgaccccct tgcgctgctc gcgcaggctc gcgatcgtcg
     1621 cggccacctg ctggcgctgg ctcggcgcgc cgcgcctggc cgcgaccgcg ctgacgagcc
     1681 gcgcaatcgg caggccacgc cgcgtgatcg tcacctgctc gccctgctcg acctcggcca
     1741 gcagctccga gagccgggtc ttggcttcgt agacggcaat ttccttcatg gtcaacctga
     1801 tttgtcagtt ggttaaccag attgtagcag ccgcctagtt cacccacacc cgcgcgttcg
     1861 cgaacatgcg cgtccacggg ctcgcggcgc tgcgctcgcc gctggtccag ctcatctgca
     1921 ggttgcggaa cacgcgctcg gcgtgcggca tcagcgcggt gaagcggccg tcggccgtcg
     1981 tcaccgcggt gaggccgccg gggctgccgt tcgggttgaa cggataggct tcggtcgggt
     2041 tgccggcgtt gtcaacaaag cgcatcgcgc ggtgcaccgc cttcgggtcg ccgcgctgtg
     2101 aaaagtcggc gaagccttcg ccgtgcgcca ccgccaccgg caggcggctg ccggccatgc
     2161 cggtgaagaa gatcgacggg ctgtcgagca cctcgacgag cgacagccgc gcctcgaact
     2221 gctcgctccg gttgcgcgtg aacttcggcc acgcgctcgc gccgggaatg atgggcgcca
     2281 gcgccgccat catctggcag ccgttgcaca cgccgagcgc gaaggtgtct ccgcggttga
     2341 agaagcgcgc gaactgttcg gcgagcgcgg ggttgaacat caccgaacgg gcccagcctt
     2401 cgccggcgcc cagcgtgtcg ccgtagctga aaccgccgca cgcgacgaag ccctggaact
     2461 ggtcgaggcg ggcgcggccg gcctgcaggt cgctcatgtg cacgtcgaag gtctcgaagc
     2521 cggcgcggtc catcgcgtag ctcatctcga ggtggctgtt gacgccctgc tcgcgcagga
     2581 tcgcgagctt ggggcgcgcc ttgccgatga acggcgcggc cacgtcgacc gcggggtcga
     2641 aactcagcgc gacatgcagg cccgggtcgt cgacacgacc cgcggcttcg tgctcggcat
     2701 cggcgccggc cgggttgtcg cgcagcttgg cgatgcgcca gctgacctcg tcccaggcct
     2761 ggtgcaagtc acgcaacggc gcgctgaaca ccgccttcgc gtcgcgccac acctccatca
     2821 cgccgcgctc gttcggcttg ccgatgaagt ggctgtgctt gctcaggccg tgctcgcgca
     2881 gcagctgcat cacctcgttg cgcaccgcgg tcggcacctg gatcaccgcg ccgagctctt
     2941 cgctgaacag cgcgcgcagc gtcagctcgt ggcggcgcgc gccgacctgc gccgcccagt
     3001 tcttcgagtc gccgtattcg gcgcggctgt cggagatgcc atcgccttcg gtgacgagca
     3061 tgtcgatgtt caggctcacg cccaagtggc cggcgaaggc catctcgcac gccgccgccc
     3121 acagcccccc gtcgctgcgg tcgtggtacg cgagcaggcg gccctgcgcg cgcagctggt
     3181 tgatcgcggc gaccagcgcc ttcagctgcg ccgggtcgtc gagatcgggc acgccatcgt
     3241 cggccagcga gccgaactgg ttcagcacct gcgccagcat cgagccggcc aggcggttgc
     3301 ggcccgcgcc gaggtcgatc aggatcagcg tcgtgtcgcc gggctgcagc tgcggcgtca
     3361 gcgtcgcccg cacgtcgtcc agcgtcgcga acgcggtgac gatcaggctg accggcgcgg
     3421 tgacctgctt cgcttcatcg ccatcgcgcc agcgcgtgcg catcgacagc gaatccttgc
     3481 cgaccggcac gccgatgccg agcgccgggc acagttccat gccgacggcc gcgacggtgt
     3541 cgtacagcgc cgcgtcttca cccggctcgc cgcacgcggc catccagttg cagctgagct
     3601 tgacgcgcgc gagttcgatc ggcgcggcca gcagattcgt gatcgcctcg ccgacggcca
     3661 tgcggcccga ggccggcgca tccagcgcgg ccagcggcgt gcgctcgccg atcgccatcg
     3721 cctcgccgcg gaagccgctg aaatcggcca gcgtcaccgc gcaatcggcc accggcacct
     3781 gccacgggcc gaccatcggg tcgcggctgc tgaggccccc gacggtgcgg tcgccgatcg
     3841 tgatcaggaa gcgcttgctc gcgaccgtcg ggtgccgcag cacgtcgcac gcgacctgct
     3901 gcagcgtcac gtcgttcaga tcgagcttcg gcgcttcgcg cgcgacgcgc tgcacgtcgc
     3961 gcagcatctt cggcggcttg ccgagcagca cgtccatcgg catcgcgatc gactgctcgc
     4021 cgcccgggcc gtcctccaag atcagctcct tcgcggcggt ggccacgccg acgaccgcga
     4081 acgggcagcg ctcgcgctcg gccatcgcgc ggaagatcgg cagcgcctcg ggcgccaccg
     4141 ccatcacgta gcgctcctgg ctctcgttgc accagatctc cttcggcgcg aggccggtct
     4201 cttcgagcgg cacctggcgc aggtcgaagc gcgcgccgac gccggcgcca tcgacgagtt
     4261 cggggaaggc gttgctgatg ccgccggcgc cgacatcgtg gatcgccagg atcgggttgc
     4321 tctcgccgag cgcccagcag tggttgatga cctcctgcgc gcggcgctcg atctcggggt
     4381 tgccgcgctg caccgaatcg aagtcgagcg ccgcggcgtt cgcgccggct gccatcgagc
     4441 tcgccgcgcc gccgcccatg ccgatgcgca tgcccgggcc gccgagctgg atcagcaagg
     4501 tgccgggcgg gaacgcgatc ttcttcgtct gcgacgccgc gatcgagccg aggccgccgg
     4561 cgatcatgat cggcttgtga taaccgcgcc gctggccggc cacgtcctgc tcgtagacgc
     4621 ggaagtagcc gccgaggttg ggccggccga actcgttgtt gaacgcggcg ccaccgagcg
     4681 ggccgtcgat catgatctgc aacgggctgg cgatgtgctc gggcttgccg atcggcgcct
     4741 gctcccacgg ctcgttcgtt ccgggcaggt gcaggttcga gacgctgaac ccggtgatgc
     4801 cggccttggg cttcgagccg cgcccggtcg cgccctcgtc gcggatctcg ccgccggcgc
     4861 cggtcgccgc acccgggaac ggcgagatcg ccgtcgggtg gttgtgcgtc tcgaccttca
     4921 tcagcacatg gaccggctcg gtgcgcgctg cgtacaccgg cgcgttcgtg tagccctgcg
     4981 gcagccagcg ctcgatcgtg ccgccttcca tcaccgacgc gttgtcgctg tacgcgacga
     5041 tcgtgtgctg cgg
//
LOCUS       DASRDG010000078         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1518777, whole genome shotgun sequence.
ACCESSION   DASRDG010000078 DASRDG010000000
VERSION     DASRDG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518777"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VFA35_01315"
     CDS             <1..1492
                     /locus_tag="VFA35_01315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZT54836.1"
                     /translation="EAADFAKGTSSRATKLVHGGVRYLAQGNVALVREALRERATLLA
                     NAPHLAQPLAFVMPTYRWWEPLFYGVGLKVYDALAGRRGLGATSVLSARRTVELLPNV
                     TQDSLAGGVKYWDGQFDDARLAVTLARTAIGQGAVVLNHCPATGLLREAGRVRGVTAR
                     DAETGEAFLLRAQCVVNATGVWVDAVRDMDREPGSTAAPPMVAPSQGVHLVVDREFLP
                     GAHALIVPKTSDGRVLFAVPWLGKTILGTTDTPRADIPAEPNASDAEIDFILHEAAGY
                     LARAPRRADVRSVWAGLRPLVKPSADEAGSTQAISREHTVVVGRSGLVTVTGGKWTTY
                     RAMAEDVLERCFESKLLPRRGGADATSGLRLAGAPSQASGSIEQPPGEHLYGTDAGTL
                     RALPGADRWLWRDGSGGFSEAMLRFAVRHEMARSIEDVLARRCRLLFLDAARAQSLAR
                     PVAAVLQEELGRPVAEAEISAFEALAQRYRGIAEAPSPTPSSRQHA"
     gene            1489..>2299
                     /locus_tag="VFA35_01320"
     CDS             1489..>2299
                     /locus_tag="VFA35_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZT54837.1"
                     /translation="MNHQDSPLAALADPTLLRTDALIGAEWIAGSARFDVTDPATGQR
                     LAEVANLGADHAQAAIDAAARAGAAWRRKPAKERCAVMLKWFRLLQEHAEDLARIMSA
                     EQGKPLAEARGEVAFGASFIEWFAEEARRVYGETIATTDPDKRYLVVRQPIGVCAAIT
                     PWNFPIAMITRKVAPAIAAGCTVVVKPAEQTPLCALAVAGLALQAGLPPGVLNVVTGD
                     GAHSIEIGRRWCESDVVQHLSFTGSTEVGRILMRQCAPTIKKLSLELGGNAP"
BASE COUNT          319 a          823 c          832 g          325 t
ORIGIN      
        1 cgaggcggcc gatttcgcca agggcacgtc gtcgcgtgcg acgaagctcg tgcacggcgg
       61 cgtgcgctac cttgcgcagg gcaatgtcgc gctggtgcgc gaggccctgc gcgagcgggc
      121 cacgctgctc gccaacgcac cgcatctcgc gcagccgctg gcgttcgtga tgccgaccta
      181 tcgctggtgg gaaccgctct tctacggcgt cgggctgaag gtctacgacg cgctggcggg
      241 ccgccgcgga ctcggcgcca cgtccgtgct gtcggcccgg cgcaccgtcg aactgctgcc
      301 gaatgtgacc caggactcgc tcgcgggcgg cgtgaagtac tgggacggcc agttcgacga
      361 tgcgcgcctg gccgtgacgc tggctcgcac cgccatcggc cagggtgcgg tggtgctgaa
      421 ccattgcccg gccacgggcc tgttgcgcga ggccggtcgc gttcgcggcg tgacggcgcg
      481 cgacgccgag accggcgaag ccttcctgct ccgcgcgcaa tgcgtcgtca acgcgaccgg
      541 cgtctgggtc gatgcggtgc gcgacatgga tcgcgagccc ggctcgacgg ctgcgccgcc
      601 gatggtcgcg ccgagccagg gcgtgcacct ggtcgtggac cgcgagttcc tgccgggtgc
      661 gcacgcgctg atcgtgccga agacatccga cggccgcgtg ctgttcgccg tgccgtggct
      721 cggcaagacg atcctgggca cgaccgacac gccgcgcgcc gatatcccgg cagagccgaa
      781 tgcgtcggac gcggagatcg acttcatcct gcacgaagcc gccggctacc tggcgcgggc
      841 gccgcgccgc gcggatgtgc gctcggtctg ggccggcctg cgcccgctgg tcaagccgag
      901 cgccgacgag gccggcagca cccaggcgat ctcgcgcgag cacacggtcg tcgtcggccg
      961 ctcggggctc gtcaccgtca ccggtggcaa gtggacaacc taccgcgcga tggccgagga
     1021 cgttctcgaa cgctgcttcg agtcgaagct gctgccgcgg cgcgggggcg ccgacgcgac
     1081 ctcgggcctg cggctggccg gcgcgccgtc gcaggcgagc ggcagcatcg agcagccgcc
     1141 cggcgaacac ctgtacggga ccgatgccgg caccttgcgc gcattgccgg gcgccgatcg
     1201 ctggctgtgg cgcgacggca gcggcggctt cagcgaggcc atgctgcgct tcgcggtccg
     1261 ccacgagatg gcgcgcagca tcgaggacgt tctggcgcgc cgctgccggc tgctctttct
     1321 cgatgccgcg cgcgcgcaaa gcctggctcg cccggttgcc gctgtgctgc aagaggaact
     1381 cgggcgcccg gtagccgaag ccgagatcag cgccttcgag gcgctggcgc agcgctaccg
     1441 gggcatcgcc gaggctccat ccccaacccc ttcttcgagg caacacgcat gaaccaccag
     1501 gactcacccc tggccgcgct ggccgacccc acgctgctgc gcaccgacgc gctgatcggc
     1561 gccgagtgga ttgcgggcag cgcgcgcttc gacgtgaccg atccggcgac cggccaacgt
     1621 ctcgccgagg tcgccaacct gggcgccgac cacgcgcagg cggccatcga cgccgctgcc
     1681 cgtgcgggcg ccgcttggcg ccgcaagccg gccaaggagc gctgcgcggt gatgctgaag
     1741 tggttccgac tgctgcaaga gcatgccgaa gacctcgccc gcatcatgtc cgcggagcag
     1801 ggcaagccgc tggccgaggc gcgcggcgaa gtcgctttcg gcgccagctt catcgaatgg
     1861 ttcgccgaag aggcgcggcg cgtctacggc gagacgatcg ccacgaccga tccggacaag
     1921 cgctatctcg tcgtgcgcca gccgatcggc gtgtgtgccg cgatcacgcc gtggaacttt
     1981 ccgatcgcga tgatcacgcg caaggtcgcg ccggcgatcg cggccggatg cacggtcgtc
     2041 gtcaagccgg ccgagcagac gccgctgtgc gcgctggccg tggccggcct ggcgctgcag
     2101 gcgggcctgc cgccgggggt gctcaacgtg gtcaccggcg acggcgccca ttcgatcgag
     2161 atcggtcgcc gctggtgcga aagcgatgtc gtgcagcacc tgtcgttcac cggctcgacc
     2221 gaggtcggcc gcatcctgat gcgacagtgc gcgccgacca tcaagaagct ctcgctcgaa
     2281 ctcggcggca acgcgccgt
//
LOCUS       DASRDG010000079         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1046934, whole genome shotgun sequence.
ACCESSION   DASRDG010000079 DASRDG010000000
VERSION     DASRDG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046934"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VFA35_01325"
     CDS             complement(<1..1283)
                     /locus_tag="VFA35_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZT54838.1"
                     /translation="MSIHVALNHVTQYRYDRPIHLGPQIVRLRPAPHSRTRVLSYSMR
                     VEPATHFINWQQDPQSNYLARLVFPDKTTSFRIEVDLVAEMAVLNPFDFFLEPSAEFY
                     PFAYEPEVATELAPYLIKAPETLDAPAFQAYLATIPREKTATNDWLVSLNQKLQRDIG
                     YLVRMEPGVQTPEETLVKASGSCRDTAWLLVQLLRHLGLAARFVSGYLIQLKPDVKSL
                     DGPSGTEVDFTDLHAWCEVFLPGAGWIGLDPTSGLLAGEGHIPLACSPEPGSAAPVSG
                     AIDECETTFEHSMIVTRVWEAPRVTLPYTEAQWRAIDALGHQVDNDLRRDDVRLTQGG
                     EPTFVSVDDREGAEWNTEAMGPTKRLLSADLMDKLRAKYGEGGLLHYGQGKWYPGEQL
                     PRWSLNLFWRKDKEPVWTHPELFASEHQDYGVTEA"
     gene            complement(1326..2300)
                     /locus_tag="VFA35_01330"
     CDS             complement(1326..2300)
                     /locus_tag="VFA35_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HZT54839.1"
                     /translation="MLSRTADHLFWMARYMERAENTARMLDVNYQASLMPQSAEDAEK
                     GWKGLLGISELSGDFAKRYGAVTPDSVMRYMVSDAENGSSIRNCLQAARENARAVRGT
                     LTTEVWETQNQTWLEFQRMVVNESFLRDPSTLFEWVKFRSHLSRGVTVGTMLQDEAFH
                     FLRIGSFLERADNTARLLDVKFHAVESEYHGFQNGNAAAARDTEVDFYHWSGILRSVS
                     GFEVYRKAYRNVIRPEKVAELLILRPDMPRSLAACMQEVVTNLKFVANEQSTETLRRA
                     GRLQADLQYGRIDEILATGLHAYLTQFLERVGTLGVGISRDFLVPVAA"
     gene            complement(2318..>3068)
                     /locus_tag="VFA35_01335"
     CDS             complement(2318..>3068)
                     /locus_tag="VFA35_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZT54840.1"
                     /translation="GTYNSAYFEHSFLARSMGIEIVEGRDLVTQNSKVYMRTTKGLKP
                     VDVIYRRINDDFLDPEAFRPDSMLGVPGLMRAYFAGNVALANAIGTGVADDKSIYPYV
                     PKMIEFYLGETPILHNVPTYMCRKDEDLKYVLANLDELVVKEVHGAGGYGMLIGPAAT
                     RAEIEDFRKALLANPSGYIAQPTLSLSTCPTYVDSGIAPRHIDLRPFVLSGKEVQMVP
                     GGLTRVALKEGSLVVNSSQGGGTKDTWVLEA"
BASE COUNT          460 a          991 c         1065 g          552 t
ORIGIN      
        1 tgcgcctcgg tgacgccgta gtcctggtgc tcgctcgcga acagctcggg gtgggtccag
       61 accggttcct tgtccttgcg ccagaacagg ttcagcgacc agcgcggcag ctgctcgccc
      121 gggtaccact tgccctgccc gtagtgcagc aggccgcctt cgccgtactt ggcgcgcagc
      181 ttgtccatca ggtcggcact gagcaggcgc ttggtcgggc ccatcgcctc ggtgttccac
      241 tccgcgcctt cgcggtcgtc gaccgagacg aaggtgggct cgccgccctg cgtcagccgc
      301 acgtcgtcgc ggcgcaggtc attgtcgacc tggtgcccga gcgcgtcgat cgcgcgccat
      361 tgcgcttcgg tgtacggcag cgtgacgcgc ggcgcctccc agacgcgcgt gacgatcatc
      421 gagtgctcga aggtggtctc gcactcgtcg atcgcgccgc tgaccggcgc ggccgagccg
      481 ggctcgggcg agcacgcgag cgggatgtgg ccctcgccgg cgagcaggcc cgaggtggga
      541 tccaggccga tccagccggc gccgggcagg aacacctcgc accaggcgtg caggtcggtg
      601 aagtcgacct cggtgccgct cgggccgtcg agcgacttca cgtcgggctt gagctggatc
      661 aggtagccgg agacgaagcg cgccgccagg ccgaggtggc gcagcagctg caccagcagc
      721 caggcggtgt cgcggcacga gccgctggcc ttgaccagcg tctcttccgg cgtctgcacg
      781 ccgggctcca tgcgcactag gtagccgatg tcgcgctgca gcttctggtt cagcgacacg
      841 agccagtcgt tcgtcgcggt cttctcgcgc gggatggtcg cgagataggc ctggaacgcc
      901 ggcgcgtcca gcgtctcggg cgccttgatc aggtagggcg cgagttcggt cgcgacctcg
      961 ggctcgtacg cgaacggata gaactcggcc gacggttcga ggaagaagtc gaacgggttc
     1021 agcaccgcca tctcggcgac cagatcgacc tcgatgcgaa agctcgtggt cttgtccggg
     1081 aagaccaggc gcgcgaggta gttcgactgc ggatcctgct gccagttgat gaagtgcgtg
     1141 gcgggctcga cgcgcatcga gtagctgagg acgcgagtgc gcgagtgcgg cgccgggcgc
     1201 agccgcacga tctgcgggcc gaggtggatc ggccggtcgt agcggtactg cgtgacgtga
     1261 ttcagcgcga cgtggatgga catggggttt ccggtgaggt gttcgagttc tgtggagctc
     1321 gcttcttacg ccgccaccgg cacgaggaag tcgcggctga tgccgacacc cagcgtgccg
     1381 acgcgttcga ggaattgcgt gagataggcg tgcagcccgg tcgcgaggat ctcgtcgatg
     1441 cggccgtact gcaggtcggc ctgcagccgg ccggcccggc gcagcgtctc ggtcgactgc
     1501 tcgttcgcga cgaacttcag gttcgtcacg acctcctgca tgcaggcggc gagcgagcgc
     1561 ggcatgtccg ggcgcaggat cagcaactcg gcgaccttct cgggccggat cacgttgcgg
     1621 tatgccttgc gatagacctc gaagcccgag accgagcgca ggatgcccga ccagtggtag
     1681 aagtccactt cggtgtcgcg cgcggcggcg gcgttgccgt tctggaagcc gtggtactcg
     1741 ctctcgaccg cgtggaactt cacgtcgagc agccgcgcgg tgttgtcggc gcgctcgagg
     1801 aagctgccga tgcgcaggaa gtggaaggcc tcgtcctgca gcatcgtgcc gaccgtgacg
     1861 ccgcgcgaca ggtgcgagcg gaacttgacc cactcgaaca gcgtgctcgg gtcgcgcagg
     1921 aacgactcgt tcaccaccat gcgctggaac tcgagccagg tctggttctg cgtctcccag
     1981 acctcggtgg tcagcgtgcc gcgcaccgcg cgcgcgttct cgcgcgccgc ctgcaggcag
     2041 ttgcggatgc tcgagccgtt ctcggcgtcg ctgaccatgt agcgcatcac gctgtcgggc
     2101 gtgaccgcgc cgtagcgctt cgcgaagtcg ccgctcagtt cgctgatccc gagcaggccc
     2161 ttccagccct tctcggcgtc ttccgcggat tgcggcatca gcgaggcctg gtagttcacg
     2221 tccagcatgc gggcggtgtt ctcggcccgc tccatgtagc gggccatcca gaacaggtgg
     2281 tcggcggttc gcgaaagcat tcttgttgtc tccgtgatca ggcttcgagc acccaggtgt
     2341 ccttggtccc gccgccctgc gacgagttga cgacgagcga cccttctttc agcgcgacgc
     2401 gcgtcaggcc gccggggacc atctgtacct ccttgccgct cagcacgaac gggcgcaggt
     2461 cgatgtggcg cggcgcgatg ccgctgtcga cgtaggtcgg gcaggtcgac aggctcagcg
     2521 tcggctgcgc gatgtagccg ctcgggttgg cgagcaaggc cttgcggaag tcctcgatct
     2581 cggcccgggt ggcggccggc ccgatcagca tgccgtagcc gccggcaccg tgcacctcct
     2641 tgacgacgag ttcgtcgagg ttcgccagca cgtacttcag gtcttcgtcc ttgcggcaca
     2701 tgtaggtcgg cacgttgtgc aggatcggcg tctcgccgag gtagaactcg atcatcttcg
     2761 gcacgtacgg gtagatcgac ttgtcgtccg cgacgccggt gccgatcgcg ttggccagcg
     2821 cgacattgcc tgcaaaatag gcacgcatca gcccaggtac gccaagcatg ctgtccgggc
     2881 ggaaagcttc ggggtcgagg aagtcgtcgt tgatccggcg ataaatcaca tccaccggct
     2941 tcagaccctt ggttgtccgc atgtagacct tgctgttttg cgtcacgaga tcgcgccctt
     3001 ccacgatctc gatccccatc gagcgcgcga gaaacgaatg ctcgaagtaa gccgagttgt
     3061 aggttccg
//
LOCUS       DASRDG010000080         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_817280, whole genome shotgun sequence.
ACCESSION   DASRDG010000080 DASRDG010000000
VERSION     DASRDG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817280"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VFA35_01340"
     CDS             complement(<1..944)
                     /locus_tag="VFA35_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT54841.1"
                     /translation="MPTAQVLDAPLAASATLRAAGAEPLVRFAGVQKTYDGVQLVIRQ
                     LDLDIRRGEFLTMLGPSGSGKTTALMLLAGFESPTAGDIYLDGKPITRTPPHKRNFGM
                     VFQNYALFPHLTVAQNVAYPLTVRKVSKTEQAAKVAKALAMVRLQGMGERFPSQLSGG
                     QQQRVALARALVFEPQLVLMDEPLGALDKQLREHMQIELKELHRQLGVTFVFVTHDQG
                     EALTMSDRVAVFNSGVIQQIDAADRMYEAPANRFVAGFIGDGTMLRGTLRAVQANGQC
                     EMALADGRVLGGVNVNAAAVGAEVVAGIRPERIVVHTQA"
     gene            1080..>2431
                     /gene="alaS"
                     /locus_tag="VFA35_01345"
     CDS             1080..>2431
                     /gene="alaS"
                     /locus_tag="VFA35_01345"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZT54842.1"
                     /translation="MKASEIRQTFLKFFEAKGHTIVASSPVVPGDDPTLLFTNAGMNQ
                     FKDVFLGTDRRPYSRATTAQKCIRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     KDAIHYAWELLTEVYQLPKEKLWVTVYAEDDEAYEIWNKSVGVPAERIVRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFYDHGPGVKGGPPGSPDEDGDRYIEIWNNVFMQFNRD
                     EAGVMHKLPKPSVDTGMGLERLTAVLQHVHSNYEIDTFVNLIAAAKKAVDAADAGDCD
                     IDSPSLKVIADHIRACTFTVVDGVIPSNAGRGYVLRRIARRAIRHGYKLGARRPFFHT
                     LVPALAAEMGDAYPELRREEKRAFEVLKQEEEGFFRTIANGMEILEKALSTGTKQIDG
                     DTAFKLHDTFGFPVDLTADVCRERGVTVDEARFNELLDEQKRRAREAGKFKMAQGLDY
                     "
BASE COUNT          434 a          818 c          813 g          366 t
ORIGIN      
        1 gcctgggtgt gcacgacgat gcgctcgggc cggatgcccg ccaccacctc ggcgccgacc
       61 gccgcggcgt tgacgttcac gccgccgagc acgcggccgt cggcgagcgc catctcgcat
      121 tgaccgttcg cctgcacggc gcgcagcgtg ccgcgcagca tcgtgccgtc gccgatgaag
      181 ccggcgacga aacggttcgc cggcgcttcg tacatgcggt cggcggcgtc gatctgctgg
      241 atcacgccgc tgttgaacac cgcgacgcgg tcgctcatcg tcagcgcctc gccctggtcg
      301 tgcgtcacga acacgaaggt cacgccgagc tggcggtgca gttccttcag ctcgatctgc
      361 atgtgctcgc gcagctgctt gtcgagcgcg ccgagcggct cgtccatcag caccagttgc
      421 ggctcgaaga cgagcgcgcg cgccagcgcg acgcgctgct gctgcccgcc cgagagctgc
      481 gacggaaagc gctcgcccat gccctgcagc cgcaccatcg ccagcgcctt cgcgaccttg
      541 gcggcctgct cggtcttgga caccttgcgc accgtgagcg gatacgccac gttctgcgcg
      601 accgtcaggt gcgggaacag cgcgtagttc tggaacacca tgccgaagtt gcgcttgtgc
      661 ggcggggtgc gggtgatcgg tttgccgtcg agatagatgt cgcccgcggt cggcgactcg
      721 aaaccagcca gcagcatcag cgcggtggtc ttgcccgatc ccgacggccc gagcatcgtc
      781 aggaactcgc cgcgccggat gtcgaggtcg agctggcgga tcacgagctg aacgccgtcg
      841 taggtctttt gcacgccggc gaagcggacc agcggctcgg cgccggcggc ccgcagggtg
      901 gcggatgcgg cgagcggggc gtcgagaact tgggcggttg gcatggcggg cggcttctcc
      961 gttcgacgaa gaccatactg taagcagcaa ctgtgccgag ccgtcataag cgcagcgcct
     1021 ttctataatc gaaagtttgc cggactcggc aagcgcgctg tacccaacga gagaaccgga
     1081 tgaaagcttc cgaaatccgc cagaccttcc tgaagttttt cgaggcgaag ggccacacga
     1141 tcgtcgcctc gagcccggtc gtgcccggcg acgacccgac gctgctgttc acgaacgccg
     1201 ggatgaacca gttcaaggac gtgttcctgg gcaccgaccg ccggccgtat tcgcgcgcga
     1261 ccaccgcgca gaagtgcatc cgtgccggcg gcaagcacaa cgacctggag aacgtcggct
     1321 ataccgcgcg ccaccacacc ttcttcgaga tgctgggcaa cttcagcttc ggcgactact
     1381 tcaagaagga cgcgatccac tacgcgtggg agctgctgac cgaggtctac cagctgccga
     1441 aagaaaagct ctgggtcacg gtctacgccg aggacgacga ggcctacgag atctggaaca
     1501 agagcgtggg cgtgccggcc gagcgcatcg tgcgcatcgg cgacaacaag ggcgcacgct
     1561 acgcctccga caacttctgg atgatgggcg acaccggccc gtgcggcccg tgcaccgaga
     1621 tcttctacga ccacggcccc ggggtcaaag gcggcccgcc gggctcgccc gacgaggacg
     1681 gcgaccgcta catcgagatc tggaacaacg tgttcatgca gttcaaccgc gacgaggcgg
     1741 gcgtgatgca caagctcccc aagcccagcg tggacaccgg catgggcctg gagcgcctga
     1801 ccgcggtgct gcagcacgtg cacagcaact acgagatcga caccttcgtc aacctgatcg
     1861 ccgcggcgaa gaaggccgtc gacgccgcag atgcgggtga ctgcgacatc gattcgccgt
     1921 cgctgaaggt gatcgccgac cacatccgcg cctgcacctt cacggtcgtc gacggcgtga
     1981 tcccgagcaa cgccggccgc ggctacgtgc tgcgccgcat cgcgcgccgc gcgatccgcc
     2041 acggctacaa gctcggtgcg cgccggccgt tcttccacac gctcgtgccg gcgctggcgg
     2101 ccgagatggg cgacgcctac cccgaactgc gccgcgagga gaagcgcgcg ttcgaggtgc
     2161 tgaagcagga ggaggaaggc ttcttccgca cgatcgcgaa cgggatggag attctcgaga
     2221 aggccttgtc gaccggaacg aagcagatcg acggcgacac cgcgttcaag ctgcacgaca
     2281 ccttcggctt cccggtcgat ctgacggccg atgtgtgccg cgagcgcggc gtcacggtcg
     2341 acgaggcgcg cttcaacgaa ctgctcgacg agcagaagcg gcgcgcccgc gaggccggca
     2401 agttcaagat ggcgcagggg ctggactaca g
//
LOCUS       DASRDG010000081         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1393544, whole genome shotgun sequence.
ACCESSION   DASRDG010000081 DASRDG010000000
VERSION     DASRDG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393544"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFA35_01350"
     CDS             complement(<1..215)
                     /locus_tag="VFA35_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZT54843.1"
                     /translation="MNTPTFTRAARLPAILAQRIVVLDGAMGTMIQRYKLAEADYRGA
                     RFADHPKDLKGNNDMLQLTKPEVITEI"
     gene            complement(245..>2092)
                     /locus_tag="VFA35_01355"
     CDS             complement(245..>2092)
                     /locus_tag="VFA35_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZT54844.1"
                     /translation="RLLQLNPAERALLLYGTLARYQRDLRGLLVEFKVSNAQEAYAAI
                     ARVAGVSETDVAEALRAGSRLERVGMVENLISEHNITDLADLMKVSEQLPPVLMRRYE
                     GPSDLMAVFTRPAKRSELSVTDFDFVGVDQKTMTALLANAVARKEPGVNVLLYGPPGT
                     GKTELAKVAAQSAGLELYEVEYADRDGNSLSGRDRYRSLQISQVFLKGSSNVALLFDE
                     VEDVFPPISTDAAQLMARMDTGDGAALTGSVSGKAWVNQILETNPVPVIWVTNRIEQI
                     DPAFRRRFQYHLELKSPPPGARESLVARALAGVEVKEGFAAKLAERKGLTPAQIRTAV
                     KFARLASSPEVGHDLQSLIERQLVNADKALGNPLVERGARRVVTQYDLGLVNTESRFE
                     VPRIVEALRRRTYGTLCFYGPPGTGKTALAEHIAHELQRPLMIRQASDIMSKYVGETE
                     QNMAAMFKEAEAEKAVLLLDEADSFLQDRRGAQRTYEVTEVNEMLQGMERFAGVFICT
                     TNLFQELDEAALRRFTFKVQFKPLTQPQRERMFVVEALGGDAAALTREQISRLAQLTS
                     LAPGDFAAVKRQVDVLGEAFAPDEFLSQLEAEHRVKPEVRQQRGMGFLH"
BASE COUNT          307 a          722 c          702 g          361 t
ORIGIN      
        1 tggatctcgg tgatcacctc gggcttggtg agctgcaaca tgtcgttgtt gcccttcagg
       61 tccttcggat gatcggcaaa gcgcgcgccg cggtaatcgg cctcggcgag cttgtagcgc
      121 tggatcatcg tgcccatcgc cccgtcgagc acgacgatgc gttgcgcgag gatcgcgggc
      181 aagcgcgcag cgcgcgtgaa ggtcggcgtg ttcattcgag cattgtagaa aacatcgcga
      241 gccctcaatg cagaaagccc atcccgcgct gctgccgcac ctcgggcttg acccgatgct
      301 cggcttcgag ctgcgacagg aactcgtcgg gcgcgaaggc ctcgccgagc acgtcgacct
      361 ggcgcttcac cgcggcgaaa tcgcccggcg cgagcgacgt gagctgcgcc aggcggctga
      421 tctgctcgcg cgtgagcgcc gccgcatcgc cgccgagcgc ctcgaccacg aacatgcgtt
      481 cgcgctgcgg ctgcgtcagc ggcttgaact gcaccttgaa cgtgaaccgg cgcagcgcgg
      541 cttcgtccag ctcctggaac aggttggtgg tgcagatgaa cacgccggcg aagcgctcca
      601 tgccctgcag catctcgttg acctcggtca cttcgtaggt gcgctgggcg ccgcggcggt
      661 cctgcaggaa gctgtcggcc tcgtcgagca gcagcaccgc cttctcggcc tcggcctcct
      721 tgaacatggc cgccatgttc tgctcggtct cgccgacgta cttgctcatg atgtcgctgg
      781 cctggcggat catcagcggg cgctgcagtt cgtgcgcgat gtgctcggcc agcgcggtct
      841 tgccggtgcc gggcgggccg tagaagcaca gcgtgccata ggtcctgcgg cgcagggcct
      901 cgacgatcct gggcacctcg aagcgcgact cggtgttgac gaggccgagg tcgtactgag
      961 tgacgacgcg ccgcgcgccg cgctcgacca aggggttgcc gagcgccttg tcggcgttga
     1021 cgagctggcg ctcgatcagc gactgcaggt cgtgccccac ctcgggcgag ctcgccaggc
     1081 gtgcgaactt caccgcggtg cggatctgcg ccggcgtcag ccccttgcgc tcggccagct
     1141 tggcggcgaa gccttccttc acctcgaccc cggccagcgc gcgcgccacc agcgactcgc
     1201 gcgcgccggg cggcgggctc ttcagctcga ggtggtactg gaagcggcgc cggaatgccg
     1261 ggtcgatctg ctcgatgcgg ttcgtgaccc agatcacggg caccgggttg gtctcgagga
     1321 tctgattcac ccaggccttg ccgctgacgc tcccggtcag cgccgcgccg tcgccggtgt
     1381 ccatgcgcgc catcagctgc gcggcgtcgg tcgagatcgg cgggaacacg tcttcgacct
     1441 cgtcgaacag cagcgcgaca ttgctgctgc ccttcaggaa cacctggctg atctgcagcg
     1501 agcggtagcg atcgcggccg ctgagcgaat tgccgtcgcg gtcggcgtat tcgacctcgt
     1561 agagttcgag cccggccgac tgcgcggcga ccttcgcgag ctcggtcttg ccggttcccg
     1621 gcgggccgta gagcagcacg ttgacgccgg gctccttgcg cgcgaccgcg ttcgccagca
     1681 gcgcggtcat cgtcttctgg tcgacgccga cgaagtcgaa atcggtcacg ctgagttcgc
     1741 tgcgcttggc cggccgcgtg aacaccgcca tcagatccga cggcccctcg taccggcgca
     1801 tcagcaccgg cggcaactgc tcgctgacct tcatcaggtc cgcgaggtcg gtgatgttgt
     1861 gctcggaaat caggttctcg accatgccga cgcgctccag ccgcgagccg gcacgcagcg
     1921 cctcggccac gtcggtctcg ctgacgccgg ccacgcgcgc aatcgcggca tacgcctcct
     1981 gcgcgttcga aaccttgaac tcgaccagca agccgcgcag atcgcgctga tagcgcgcca
     2041 gcgtgccgta cagcagcaac gcacgctcgg ccggattgag ttgcagcagc cg
//
LOCUS       DASRDG010000082         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1278342, whole genome shotgun sequence.
ACCESSION   DASRDG010000082 DASRDG010000000
VERSION     DASRDG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278342"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /locus_tag="VFA35_01360"
     CDS             <1..1898
                     /locus_tag="VFA35_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54845.1"
                     /translation="ILQKILDRGTEQEDREKRLLRGLVITTLYMEHDPRLLRALRSSL
                     GHTDPMVVDWTLRSLGVHGTEDEADAVLQLLGWKGGIYRQTALASLSRMRAPKALQIC
                     TQLAGGKDMTRSELCALQVGLRQRGGAEALLQALAANADAVVAAGAKVPLPPAAAGPA
                     PGAEGVKVKLTLGDKERTVLEGRLVGFLGDSARVSGAVKGLPEAEIAFADCNVFDFPD
                     HAVQPITNTRVFLRQGSLVTGRLVAMDEKDIRLDSPVFHELVIPRGELQGIAVDPGLD
                     RLLGASTEHDRVRLKSNEFLDGTIKSLVDGKINVALSAGGERQVPLADVAGLLTTRPK
                     TLEPDATVYVRIDLVNGDRVIGFLVGSTPTHLAFTAPLIGAAVVPITSVARVEIGVGG
                     GAMWGFTLIADYSDNRVVEVDEQGKITWELDDVFGAWDAVCLDNGNILITEFSVSRVS
                     EVDRKGNRLWVYEDLKNPYCADRLPNGNTLIGDTFGGRVIEVSPQKEIVWKYDKEIRP
                     FDAKRLANGNTLISDVLKDRVIEVSPQGEIVWEVKNMNNVHDADRLPNGNTLITLRSK
                     GQVIEVDREGKVVWELNGLMSPSDADRLPNGNTLVAENNAVREFDRHGNVVWKKEMTW
                     AVEANRY"
     gene            complement(1976..>2221)
                     /locus_tag="VFA35_01365"
     CDS             complement(1976..>2221)
                     /locus_tag="VFA35_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54846.1"
                     /translation="FAADGWSRGVATLRAGGDSAAVAARAWFALAVEGAPRDPMAQSL
                     LLYCEAARDHDAARQQRVADHVRALGVPPALAPVFGG"
BASE COUNT          362 a          781 c          786 g          292 t
ORIGIN      
        1 gcatcctgca gaagatcctc gaccgcggca ccgagcagga ggatcgcgag aagcgtctgc
       61 tgcgcggact ggtgatcacg acgctctaca tggagcacga cccgcggctc ctgcgcgcgc
      121 tgcgctcgtc gctcggccac accgacccga tggtcgtcga ctggacgctg cgttcgctcg
      181 gtgtgcacgg caccgaggac gaggccgacg cggtgctgca gctgctcggc tggaagggcg
      241 gcatctaccg ccagaccgca ctcgcgtccc tgtcgcgcat gcgcgcgccg aaggcgctgc
      301 agatctgcac gcagctcgcg ggcggcaagg acatgaccag gtccgagctc tgcgcgctgc
      361 aggtcggtct gcgccagcgc ggcggcgccg aggcgctgct gcaggcgctc gccgccaacg
      421 ccgatgccgt cgtcgcggcg ggcgcgaagg tgccgctgcc gccggcggcg gcgggcccgg
      481 cgccgggcgc cgagggcgtc aaggtgaagc tcacgctcgg cgacaaggag cgcaccgtgc
      541 tcgaaggccg cctcgtcggc ttcctcggcg attcggcgcg cgtgagcggg gccgtcaagg
      601 gcctgcccga ggcggagatc gcgttcgccg actgcaacgt gttcgacttc cccgaccacg
      661 ccgtgcagcc gatcacgaac acgcgcgtgt tcctgcgcca gggcagcctc gtgaccggca
      721 ggctcgtggc gatggacgag aaggacatcc ggctcgactc gccggtgttc cacgagctcg
      781 tgatcccgcg cggcgaattg cagggcatcg ccgtcgaccc cggcctcgac cgcctgctcg
      841 gcgcctcgac ggagcacgac cgcgtgcgcc tgaagtccaa cgagttcctc gacggcacca
      901 tcaagtcgct cgtcgacggc aagatcaacg tggcgctgag cgcgggcggc gaacgccagg
      961 tgccgctcgc cgacgtcgcg ggcctgctga ccacgcggcc gaagacgctc gagcccgacg
     1021 cgaccgtcta cgtgcgcatc gacctcgtga acggcgaccg cgtgatcggc ttcctcgtcg
     1081 gcagcacgcc gacgcacctc gcgttcaccg cgccgctgat cggcgccgcg gtggtgccga
     1141 tcacgtccgt cgcgcgcgtc gagatcggcg tcggcggcgg cgcgatgtgg ggcttcacgc
     1201 tgatcgccga ctacagcgac aaccgcgtgg tcgaggtcga cgagcagggc aagatcacct
     1261 gggagctcga cgacgtgttc ggcgcgtggg atgccgtgtg cctggacaac ggcaacatcc
     1321 tgatcaccga gttctcggtc agccgcgtct ccgaggtcga ccgcaagggc aaccgcctct
     1381 gggtctacga ggatctcaag aacccgtact gcgccgaccg cctgcccaac ggcaacacgc
     1441 tgatcggcga cacgttcggc ggccgcgtga tcgaggtgtc gccgcagaag gagatcgtct
     1501 ggaagtacga caaggagatc cgtccgttcg acgccaagcg gctcgcgaac ggcaacacgc
     1561 tgatctccga cgtgctgaag gaccgcgtga tcgaggtgag cccccagggc gagatcgtct
     1621 gggaggtgaa gaacatgaac aacgtgcacg acgccgaccg cctgcccaac ggcaacacgt
     1681 tgatcacgct gcgctccaag ggccaggtca tcgaggtcga ccgcgaaggc aaggtggtct
     1741 gggagctcaa cggcctgatg tcgccgagcg acgccgaccg cctgcccaac ggcaacacgc
     1801 tggtcgccga gaacaacgcg gtgcgcgagt tcgaccggca cggcaacgtc gtctggaaga
     1861 aggagatgac ctgggccgtg gaggcgaacc gctactgagg cgcggacgcc gaaggcggcc
     1921 tcgcctcagg attccttctc cgtctctgaa ggtgcggacg ccgaaggcgt ggcccctacc
     1981 cgccgaacac gggcgcgagc gcgggcggca cgccgagcgc gcgcacgtgg tcggcgaccc
     2041 gctgctggcg cgcggcatcg tgatcgcgcg ccgcttcgca gtagagcagg aggctctggg
     2101 ccatcggatc gcgcggcgcg ccctcgaccg ccagcgcgaa ccacgcgcgc gccgcgaccg
     2161 ccgccgaatc gccgccggcg cggagcgtcg cgacgccgcg gctccagccg tcggccgcga
     2221 a
//
LOCUS       DASRDG010000083         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1047449, whole genome shotgun sequence.
ACCESSION   DASRDG010000083 DASRDG010000000
VERSION     DASRDG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047449"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VFA35_01370"
     CDS             <1..1231
                     /locus_tag="VFA35_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HZT54847.1"
                     /translation="PPALRPALARTKGVPIFQEQVMQIAMIAADFSAGEADGLRRAMA
                     AWKRKGGLGHYHERLVGRMIAKGYDAEFAERIFRQIQGFGEYGFPESHAAGFAQLAYA
                     SCWVKRHHPDAFLAALLNSQPMGFYAPAQLVRDARAHGVEVRPVDVTVSAGESTLEPM
                     AVPGALHAVRLGLDRVGGLSAEAAQRIVAARAQAAFTGPEDLARRAALDARALQCLAR
                     ADALRALTGHRHQAAWAVAGIDTRPTPLLRATRFDERAVALPAPAEAEETLADYRSLG
                     LTLNRHPLALLREQLAAFRVQPAAVLRGYPNGRLARASGLVTHRQRPETAKGTMFVTL
                     EDDTGTVNAIVWPAVVETQRKPLLAARLLTLFGRWQRQDGPQGEGDDAVMHLVVMKAI
                     DHSALLQGLVSRSRDFR"
     gene            1271..2053
                     /gene="yaaA"
                     /locus_tag="VFA35_01375"
     CDS             1271..2053
                     /gene="yaaA"
                     /locus_tag="VFA35_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HZT54848.1"
                     /translation="MLYLLSPAKSLDYATPTPRAIAKLATDPLFTERAAELIGLMRRR
                     SAAEVAEMMDLSDALATLNVARYHAWSPCFTPANSKPAALAFDGDVYGGLRAPELGLA
                     DLRWAQKHVAILSGLHGVLRPLDRLQPYRLEMGTRLPNPHGADLYAYWGDTIAQHLND
                     RAAEHAAPVIVNLASTEYFRAADRRALTPRVIECVFEDWKNGRYKIISFFAKRARGLM
                     ARHAITQRIATPAGLKKFDAEGYRFDAAVSERDRLVFRRRLD"
     gene            2100..2954
                     /locus_tag="VFA35_01380"
     CDS             2100..2954
                     /locus_tag="VFA35_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZT54849.1"
                     /translation="MSEQQPITPELRAWIIEQATAGHRPEAVLQSMRASGWNEDSAIA
                     ALESVLAGYLDEKARAAALPPPVRVPDPLPQGTRTTLWAGDREVQVLVNLLTPRVVVF
                     GQLLSDDECDAVIALAAKRLARSETVQTETGGSEVNEARTSQGMFFERGEHPVVARIE
                     RRMAALLDWPLENGEGLQVLHYKPGAEYKPHYDYFDPVHPGSATILQRGGQRVGTLVM
                     YLNTPKKGGGTTFPDVGLEVAPIKGNAVFFSYDRPHVSTRSLHGGAPVIEGEKWVATK
                     WLREREFI"
BASE COUNT          449 a         1140 c         1067 g          400 t
ORIGIN      
        1 ccctcccgcg ttgcgcccgg cgctcgcgcg caccaagggc gtgccgatct ttcaggagca
       61 ggtcatgcag atcgcgatga tcgcggccga cttcagcgcc ggtgaagccg atggcctgcg
      121 ccgcgcaatg gccgcctgga agcgcaaggg cggcctcggc cactaccacg agaggctcgt
      181 cggccgcatg atcgccaagg gctacgacgc cgaatttgcc gagcgcatct tcaggcagat
      241 ccagggcttc ggcgaatacg gtttccccga gagccacgcg gcgggtttcg cacaactggc
      301 ttacgcgagt tgctgggtca agcggcatca ccccgatgcg ttcctggccg cgctgttgaa
      361 cagccagccg atgggcttct acgctcccgc gcagctggtg cgcgatgcgc gcgcgcacgg
      421 cgtcgaagtg cggccggtgg acgtgacggt gagcgcgggc gagagcacgc tcgagcccat
      481 ggccgtgccg ggcgcgttgc acgcggtgcg gctcggcctc gatcgcgtcg gcgggctgtc
      541 ggccgaggcg gcgcagcgca tcgtcgcggc ccgtgcgcag gcggcgttca cgggccccga
      601 agacctcgcc cgccgcgccg cgctcgacgc ccgcgcgctg caatgcctcg cgcgcgccga
      661 tgcgctgcgc gcgctcaccg gccaccgcca ccaggccgcg tgggccgtgg ccggcatcga
      721 cacgcggccg acgccgctgc tgcgcgccac gaggttcgac gaacgcgccg tcgccttgcc
      781 cgcgcccgcc gaggccgagg agacgctggc cgactaccgc agcctcggcc tcacgctgaa
      841 ccggcacccg ctcgcgttgc tgcgcgagca gctcgcggcc ttccgcgtcc agcccgccgc
      901 ggtgctgcgc ggctacccga acggccgcct cgcgcgcgcc agcggcctcg tcacgcaccg
      961 gcagcgcccc gagaccgcga agggcacgat gttcgtcacg ctcgaggacg acaccggcac
     1021 cgtcaacgcg atcgtctggc cggcggtggt cgagacgcag cgcaaaccgc tgctcgcggc
     1081 gcggctgctc acgctgttcg ggcgctggca gcgccaggac gggccgcagg gcgagggcga
     1141 cgacgcggtg atgcacctgg tcgtgatgaa ggcgatcgac cactccgcgc tgctgcaagg
     1201 gctggtcagc cgcagccggg acttccgctg agcacgttgc cgcgagtcac cggccgcaat
     1261 aatcgcggcc atgctgtatt tgctgtctcc cgccaagtcg ctcgactacg ccacgccgac
     1321 gccgcgcgcc atcgcgaagc tcgccaccga cccgctgttc accgagcgcg ccgccgagct
     1381 gatcggcctg atgcgccggc gcagcgccgc cgaggtcgcc gagatgatgg acctctcgga
     1441 cgcactcgcc acgctcaacg tcgcgcgcta ccacgcctgg tcgccctgct tcacgcccgc
     1501 caacagcaag cccgccgcgc tcgcgttcga cggcgacgtc tacggcggcc tgcgcgcgcc
     1561 cgagctcggc ctcgccgacc tgcgctgggc gcagaagcac gtggcgatcc tgagcggcct
     1621 gcacggcgtg ctgcgcccgc tcgaccggct gcagccgtac cggctcgaga tgggcacgcg
     1681 cctgccgaac ccgcacggcg ccgatctcta cgcctactgg ggcgacacga tcgcgcagca
     1741 cctgaacgat cgcgctgccg aacacgccgc gccggtgatc gtgaacctcg cgtcgaccga
     1801 gtacttccgc gccgccgacc gtcgcgcgct cacgccgcgc gtgatcgagt gcgtgttcga
     1861 ggactggaag aacggccgct acaagatcat cagcttcttc gccaagcgcg cgcgcgggct
     1921 gatggcgcgg cacgcgatca cgcagcgcat cgcgacgccg gcgggcctga agaagttcga
     1981 tgccgagggc taccgcttcg acgcggcggt ctccgagcgc gaccggctgg tgttccggcg
     2041 ccggctcgac tgacgaacgg aagcacaccc gagaaaacac atccggcaaa atccggcgca
     2101 tgagcgagca gcagcccatc acgcccgaac tgcgcgcgtg gatcatcgag caggccaccg
     2161 ccgggcaccg gcccgaggcc gtgctgcagt cgatgcgggc cagcggctgg aacgaggact
     2221 cggcgatcgc cgcgctcgag agcgtgctcg ccggttacct cgacgagaag gcccgggccg
     2281 cggcgttgcc acccccggtg cgcgtgcccg acccgctgcc gcagggcacg cgcacgacgc
     2341 tgtgggccgg cgaccgcgag gtgcaggtgc tggtgaacct gctgactccg cgcgtcgtcg
     2401 tgttcggcca gctgctgtcg gacgacgaat gcgacgcggt gatcgcgctc gccgccaagc
     2461 gactcgcgcg ctccgagacc gtgcagaccg aaaccggcgg cagcgaggtc aacgaggcgc
     2521 gcaccagcca gggcatgttc ttcgagcgcg gcgagcaccc ggtcgtcgcg cgcatcgagc
     2581 gccgcatggc tgcgctgctc gactggccgc tcgagaacgg cgagggcctg caggtgctgc
     2641 actacaagcc gggcgccgag tacaagccgc actacgacta cttcgacccg gtgcatcccg
     2701 gctcggccac gatcctgcag cgcggcggcc agcgcgtcgg cacgctggtg atgtacctga
     2761 acacgccgaa gaagggcggg ggcaccacct tcccggacgt cggcctcgag gtcgcgccga
     2821 tcaagggcaa cgcggtgttc ttcagctacg accgcccgca cgtctcgacg cgcagcctgc
     2881 acggcggcgc gccggtgatc gaaggcgaga agtgggtcgc caccaagtgg ctgcgcgagc
     2941 gtgagttcat ctgacgcccg cgcggccgca ggcgccggcg ggtcgccctc gtcgtcgggc
     3001 ccgctcgcgg gcctgtgcgt cctcgatctc acacgcctgt tgccggggcc gctcgc
//
LOCUS       DASRDG010000084         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_817629, whole genome shotgun sequence.
ACCESSION   DASRDG010000084 DASRDG010000000
VERSION     DASRDG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817629"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFA35_01385"
     CDS             complement(<1..462)
                     /locus_tag="VFA35_01385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZT54850.1"
                     /translation="MNPPLRAVAPSFSAREFRAALGMFATGVTIITARRIDGTPIGLT
                     ANSFNSVSLAPPLVLWSLARSAGSMPHFERGSHYAINILAAEQHALAERFASKALDRF
                     ADLAFREGAGGAPILEGAAAVFECFNRSRYEEGDHVIFVGEVERCERRTGAQ"
     gene            509..1093
                     /locus_tag="VFA35_01390"
     CDS             509..1093
                     /locus_tag="VFA35_01390"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZT54851.1"
                     /translation="MEPLARHLLSQAFNNAWANHRLLGACAQLNPPEFEAPRTGFFGS
                     IKATLNHALTVDWYYIDAIERGLASQAPNGAFMAFYTPEEPFADCAALWAAQRASDRR
                     LIALCEPLDAAALATIIVVPRRAGLTHEPLPRLLAHLFQHQVHHRGQVQAMLSGTFVA
                     PPQLDEFFCTWDAPLRADDFRALGFSEAAIWPAE"
     gene            1096..1674
                     /locus_tag="VFA35_01395"
     CDS             1096..1674
                     /locus_tag="VFA35_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT54852.1"
                     /translation="MRDPLFIELPTRIDTERLLMRPPQAGDGPQFFEALSESLAELRR
                     FLSALAWVAAEPSLEASELYCRTGQANFVARKDLPFFLFERASGQLVGATGLHRTAWG
                     TPKTEVGYWVRSSRSGQGLMAEAVEATTRYAFAHIGAVRVELITDAANQASRKVADRC
                     GFRLEGTLVHDRRAPDGSLRSTCIYARFPPTC"
     gene            1705..2478
                     /locus_tag="VFA35_01400"
     CDS             1705..2478
                     /locus_tag="VFA35_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004539528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT54853.1"
                     /translation="MTTLASPLPAPPATAEDLAAITTAQWRAVLAAAPADAAGWITAA
                     AQLGHAEAQAVLGQWQLDGHGVAPDPAQALHWFLRAAQRGHAMAMNMAGRCHEQGWGT
                     QPDAGKAAHWYRQALRLGLPEAHYNLANLLAAGSGVACDHDQAFALYEQAAGQGYAKA
                     FAKLGRYFEDGQVVARDADEALRCYRRGAEGGDFRGQFCYAGMLAAQGREAEALHWLA
                     KVPETATPRYLKQAGALLLESTHAAFRAIGERMLARAEA"
BASE COUNT          347 a          935 c          902 g          331 t
ORIGIN      
        1 ctgagcgccg gtgcggcgct cgcagcgttc gacctcaccg acgaagatca cgtggtcgcc
       61 ttcttcatag cggctgcggt tgaagcactc gaacaccgcg gccgcgcctt cgagaatcgg
      121 cgcgccgccg gcgccctcgc gaaacgccag gtcggcgaag cggtcgagcg ccttgctcgc
      181 gaagcgctcg gccagcgcgt gctgctcggc ggcaaggatg ttgatcgcgt agtgcgagcc
      241 gcgctcgaag tgcggcatcg agccggcgct gcgggcgagg ctccacagca ccagcggcgg
      301 ggcgagcgag accgagttga acgagttggc ggtcaggccg atcggcgtgc cgtcgatgcg
      361 gcgcgcggtg atgatcgtca cgccggtcgc gaacatgccg agcgcggcgc gaaactcgcg
      421 cgccgagaag ctgggcgcca cggcgcgaag cggggggttc atgggcgcat tgtccgggat
      481 tcgcacacgc ccgcctacac tgcccggcgt ggagccgctt gcccgccacc tgctgagcca
      541 ggcgttcaac aacgcctggg cgaaccaccg cctgctcggc gcctgcgcgc agctgaaccc
      601 ccccgagttc gaggcgccgc gcaccggttt cttcggctcg atcaaggcga cgctgaacca
      661 cgcgctgacg gtcgactggt actacatcga cgcgatcgag cgcggcctcg ccagccaggc
      721 gccgaacggc gcgttcatgg cgttctacac gcccgaagag ccgttcgcgg actgcgccgc
      781 gctgtgggcc gcgcagcgcg cgagcgaccg gcggctgatc gcgctgtgcg agccgctcga
      841 cgcggcggca ctcgcgacga tcatcgtcgt gccgcgccgg gccggcctca cgcacgagcc
      901 tttgccgcgg ctgctggcgc acctgttcca gcaccaggtg catcaccgcg ggcaggtgca
      961 agcgatgctg tcgggcacct ttgtcgcgcc gccgcagctc gacgagttct tctgcacctg
     1021 ggacgccccg ctgcgcgccg acgacttccg cgccctcggc ttcagcgagg ccgcgatctg
     1081 gcccgcggag tagacatgcg tgaccccctg ttcatcgagc tgccgacccg catcgacacc
     1141 gagcgcctgc tgatgcgccc gccgcaggcc ggcgacggcc cgcagttctt cgaagcgctc
     1201 tcggagtcgc tcgccgagct gcgccggttc ctgtcggccc tggcctgggt cgcggccgag
     1261 ccgagcctgg aggcgtcgga gctgtactgc cgcaccgggc aggccaactt cgtggcgcgc
     1321 aaggacctgc cgttcttcct gttcgagcgc gcgagcggcc aactcgtggg cgccaccggg
     1381 ctgcaccgca ccgcatgggg cacgccgaag accgaggtgg gctactgggt tcgcagctcg
     1441 cgctccgggc agggcctgat ggccgaggcg gtcgaggcca ccacgcgcta cgcgttcgcg
     1501 cacatcggcg cggtccgggt cgagctcatc accgacgcgg cgaaccaggc ctcgcgcaag
     1561 gtcgccgatc gctgcggctt tcggctcgag ggcacgctgg tgcacgaccg ccgcgcgccg
     1621 gacgggagcc tgcggagcac ctgcatctac gcgcgctttc cgccgacgtg ctgacgcccg
     1681 cgcaggcagg ggacaatcgc gcccatgacg accctcgcga gcccgctacc ggcaccgccc
     1741 gccaccgccg aagacctggc cgccatcacg accgcgcagt ggcgcgcggt gctcgccgcc
     1801 gcgccggccg acgcggccgg ctggatcacc gccgccgcgc agctcggtca cgccgaggcg
     1861 caggccgtgc tcggccagtg gcagctcgac ggccatggcg tcgcgcccga cccggcgcag
     1921 gcgctgcact ggttcctgcg cgccgcgcag cgcggccacg cgatggcgat gaacatggcg
     1981 ggccgctgcc acgagcaggg ctggggcacg cagcccgacg ccggcaaggc ggcgcattgg
     2041 taccgccagg cgctgcggct cggcctgccc gaggcgcact acaacctcgc gaacctgctc
     2101 gccgccggca gcggcgtcgc gtgcgatcac gaccaggcgt tcgcgctgta cgagcaggcg
     2161 gccgggcagg gctacgcgaa ggcgttcgcc aagctcggcc gctacttcga ggacggccag
     2221 gtcgtcgcgc gcgatgccga cgaggcgctg cgctgctacc ggcgcggcgc cgagggcggc
     2281 gacttccgcg gccagttctg ctacgccggc atgctcgccg cgcagggccg cgaggccgag
     2341 gcgctgcact ggctcgccaa ggtgcccgag accgcgacac cgcgctatct gaagcaggcc
     2401 ggcgcgttgc tgctcgaatc gacgcacgcc gcgttccgcg cgatcggcga gcgcatgctg
     2461 gcgcgtgccg aagcgtgacc cgagcttgag cgcccccgat cgtttcaacg cggcc
//
LOCUS       DASRDG010000085         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_587554, whole genome shotgun sequence.
ACCESSION   DASRDG010000085 DASRDG010000000
VERSION     DASRDG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587554"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VFA35_01405"
     CDS             complement(<1..860)
                     /locus_tag="VFA35_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZT54854.1"
                     /translation="MSALICGSLAFDTIATFPGRFSQQIMPDQLHILNVSFLVPTLRR
                     EFGGCAGNIAYSLSQLGGQPVIMAAVGGDGAEYLRRVESWGATTALIRTLPDTHTAQA
                     IIITDTDNNQITAFHPGAMQSAHLSMVPQDSDIRVAIIAPDGRDAMLQHAAQLAQAKI
                     PFIFDPGQGLPMFDGAELRGFVEQATWVAVNDYEAQMLCERTGQTLESLSRSHLRGII
                     VTLGAEGCDLWQGGVRVRVPGVVADAVVDPTGCGDAFRAALLYGIEQGWPLERCVQLG
                     NRVGAIKVACR"
     gene            complement(915..1943)
                     /locus_tag="VFA35_01410"
     CDS             complement(915..1943)
                     /locus_tag="VFA35_01410"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HZT54855.1"
                     /translation="MSLATRCTACGTVFRVVQDQLKVSEGWVRCGRCSEVFNALEGLF
                     DLERDAPPQGSAGAVEAGEVPTAETGVEPDSSGDTPDGSAPVEADPTAVETTASTDAI
                     DKLDAQLLRARESGHDSTPATRVSERDRLEFPDAQFDPEMLADDTLDAQPPGAEPVPA
                     QPLAATEPPSTPSFVQHAQRNARWNTPGMRALQGVVAVVLVAALGLQVAHRFRDLIAA
                     RWPGTGPALAAWCGSAGCTIDAPRRIEDVAVESTALARASSVPDDAFRLSVVLRNRGA
                     MTVALPSVDLSLTDANGQLVSRRMLAPRDFGVASTSMRAGAEAALQLVLSTSGARVTG
                     YTVEVFYP"
BASE COUNT          307 a          727 c          692 g          287 t
ORIGIN      
        1 cggcaagcga ccttgatcgc gccgacccgg ttgccgagtt gcacgcagcg ctcgagcggc
       61 caaccctgct cgatgccgta cagcagcgcg gcgcggaacg cgtcgccgca gccggtcgga
      121 tcgaccaccg cgtccgccac cacgcccggg acgcgcacgc gaacgccgcc ctgccacagg
      181 tcgcagccct cggcgccgag cgtcacgatg atcccgcgca ggtgcgagcg cgacaacgac
      241 tccagcgtct gcccggtgcg ctcgcacagc atctgcgctt cgtagtcgtt gaccgccacc
      301 caggtcgcct gctcgacgaa gccacgcagc tccgcgccgt cgaacatcgg caggccctgg
      361 ccggggtcga agatgaacgg gatcttcgcc tgcgccagtt gcgcggcgtg ctgcagcatc
      421 gcgtcgcggc catcgggggc gatgatcgcg acccggatgt cgctgtcctg cgggaccatg
      481 ctcagatgcg ccgactgcat cgcccctggg tggaacgccg tgatctggtt gttgtcggtg
      541 tcggtgatga tgatggcctg cgcggtgtgc gtgtccggca gcgtgcggat cagcgcggtg
      601 gtcgcgcccc acgactcgac gcgtcgcagg tattcggcgc cgtcgccgcc gaccgctgcc
      661 atgatcaccg gctggccgcc gagttggctc agcgagtacg cgatgttgcc cgcgcagcca
      721 ccgaactcgc gtcgcagtgt cggcaccagg aacgagacgt tcaggatgtg caactgatcc
      781 ggcatgatct gctgggagaa acgccccggg aaggtggcaa tcgtgtcgaa ggcgagcgaa
      841 ccgcaaatca aggccgacat ggcagctcca aagttcaagg caagggcgaa ggcggtccag
      901 gccgccgccg ggtttcaggg atagaagact tcaacggtgt agccggtgac gcgcgcgccg
      961 ctcgtcgaca gaacgagttg gagcgccgct tccgcgccgg cccgcatcga tgtcgaggcg
     1021 accccgaaat cgcgcggcgc cagcatgcgg cgcgagacca gctgcccgtt cgcatcggtc
     1081 aggctcagat cgaccgaggg cagcgcgacc gtcatcgcgc cgcggttgcg caagaccacc
     1141 gacagccgga acgcgtcgtc gggcaccgac gacgcgcgcg ccagcgcggt gctctcgacc
     1201 gccacgtcct cgatccggcg cggcgcgtcg atcgtgcacc ccgcgctgcc gcaccaggcg
     1261 gccaacgccg ggcccgtgcc cggccagcgg gccgcgatca gatcgcgaaa ccgatgcgcc
     1321 acctgcagcc cgagcgcggc cacgagcacg acggcaacga caccctgcag cgcacgcatg
     1381 cccggcgtgt tccagcgcgc gttgcgctgc gcatgctgca cgaagctcgg cgtgctgggt
     1441 ggctcggttg ccgcgagcgg ctgcgccggc acgggctccg cgccgggtgg ctgcgcatcg
     1501 agagtgtcgt cggccagcat ctccgggtcg aactgggcat ccgggaactc cagccgatcg
     1561 cgttcgctga cgcgcgtcgc cggggtcgag tcatgacccg actcgcgcgc acgcagcagc
     1621 tgcgcgtcga gcttgtcgat cgcgtcggtc gatgcggtgg tctcgacggc ggtgggatcg
     1681 gcctcgaccg gtgccgagcc atcgggggtg tcgccgctcg aatccggctc gacgccggtc
     1741 tccgcggtcg gcacctcgcc cgcttctacc gcgccggcag atccttgcgg cggcgcgtcc
     1801 cgttccaggt cgaacagccc ctcgagcgcg ttgaacacct cgctgcagcg cccgcagcga
     1861 acccagccct ccgagacctt cagctgatcc tggacgacgc gaaagaccgt gccgcaggcc
     1921 gtgcaacgcg tggccaggct cattgcgcaa cgtccgccat cggtccgtct ttcaggcggc
     1981 gcggcgcgcc gtcatcagga tccagccgtc ctc
//
LOCUS       DASRDG010000086         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_128409, whole genome shotgun sequence.
ACCESSION   DASRDG010000086 DASRDG010000000
VERSION     DASRDG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128409"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2100)
                     /locus_tag="VFA35_01415"
     CDS             complement(<1..2100)
                     /locus_tag="VFA35_01415"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZT54856.1"
                     /translation="MSARAPNALRDPAPSAVAGKRAPSAKPPPAVADAAAASFAALTH
                     KLDYLDAADIKRVREAYRFADEAHLGQFRASGEPYITHPIAVAGLCAEWKLDAQAIMA
                     ALMHDAMEDCGVTKIELIEKFGAPVADLVDGLTKLDKLQFSTREESQAESFRKMLLAM
                     ARDVRVILIKLADRLHNMRTMDAMAAAKRSRIAGETLEIYAPIAHRLGLNQTYRELQE
                     LSFQHLRPWRQAALSKAVLKARGYRRDIVERIQHDVDRAFLQAKLPVQVYGREKTLFS
                     IYKKMREKHLTFAQVNDIFGFRIVVGTLMECYMALGVLHQLFKPLPGRFKDYIAIPKA
                     NGYQSLHTTLVSPLGTAIEFQIRTEQMHAVAEKGIAAHWLYKSGGAQVQEAQRLGAMW
                     LQSLIDIQDETHDANEFLEHVKIDLFPDAVYVFTPKSKILALPRGATPVDFAYAIHTD
                     VGDHTVAAKVNGEPVALRAELRSGDVVEIVSAAGARPNPAWLNFVRTGRARSKIRHYL
                     KNMEQEESQDLGEKMLAQAMRAEGLHLPGPPGDQPADAALWQQLTRWSGNKTREDLFT
                     DIGLGRKIATIVAKRLTRLMAERGTRPDALTLTMGRYVSDDDAPAQGTVLIDGSEGAS
                     VQLAPCCRPIPGDAIVGYLGRGEGLMVHTAECSVGKRLFERDSEHWMGVDWAEQPTRP
                     FETAVTLLVNNGKGVLAQ"
     gene            complement(2191..2394)
                     /gene="rpoZ"
                     /locus_tag="VFA35_01420"
     CDS             complement(2191..2394)
                     /gene="rpoZ"
                     /locus_tag="VFA35_01420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZT54857.1"
                     /translation="MARITVEDCLTKIPNRFQLVLAATYRARMLSQGHAPKIETKNKP
                     GVTALREIAAGEVGVEMLRKVPV"
     gene            complement(2418..3038)
                     /gene="gmk"
                     /locus_tag="VFA35_01425"
     CDS             complement(2418..3038)
                     /gene="gmk"
                     /locus_tag="VFA35_01425"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZT54858.1"
                     /translation="MDYPGNLFVVAAPSGAGKSSLVSALLQVDSHLVVAVSHTTRAPR
                     GQEQQDREYHFTDEPTFRAMIERGEFFEWAQVHGHLYGTSRAAIETRITGGQDVVLEI
                     DWQGALQIKQLFPNAILIFILPPSWDELQQRLNRRGEDRPEVIAKRMENARIEVSKAR
                     EFDFVIINALFETALFDLKAIVHSQRLKYAALQRSKPAVFAALDLS"
     gene            complement(3196..4116)
                     /locus_tag="VFA35_01430"
     CDS             complement(3196..4116)
                     /locus_tag="VFA35_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZT54859.1"
                     /translation="MRSALRTPVHDAGNFDDFGVDTISFAGPLEAKLKAAREAGFTQI
                     MLGARDVVGHPDGLDAAVAAVRSSGLRITGFQVLRDYEGLGGHLHQYKIDVAKSMLEM
                     CARLGAKVLLVCSSTSAHADGDIDRAARDLRKLAMLAVPLGVKAAYVALSWGRHVNEF
                     MQAWEVVTRADMPNLGIALDSFHAFATKTPAEDLELLDPYKIYLVQLADFMWQEVRSA
                     EERISTARHFRVFPGEGVHSEQVADWVTRLHQLGYRGDYSLEVFNDDYQQMPLPTVAE
                     RARRSALWLGETVLKRSVPLPNAMKLKRVV"
     gene            complement(4120..>4536)
                     /locus_tag="VFA35_01435"
     CDS             complement(4120..>4536)
                     /locus_tag="VFA35_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZT54860.1"
                     /translation="DPYPSPSLAGMRWFGIVQYVGNDRTEDWTDFYRELFGFEPVTSK
                     QAFGILPKGRVLASPCGSFYLQLIEPEPGILDVEDDEQLMRVAFGTTDVLGAVSLLRE
                     RGVEFHESERLHSEARGALTKPVLRGVSFEFVHVKE"
BASE COUNT          663 a         1508 c         1590 g          775 t
ORIGIN      
        1 ctgcgcgagc acgcccttgc cgttgttgac gagcagcgtc accgcggtct cgaacgggcg
       61 cgtgggctgt tcggcccagt ccacgcccat ccagtgctcg ctgtcgcgct cgaacagccg
      121 cttgccgacg ctgcattctg cggtgtgcac catcagccct tcgccacggc cgaggtagcc
      181 gacgatcgcg tcgcccggga tcggccggca gcacggcgcg agctgcaccg aggcgccttc
      241 gctgccgtcg atcagcaccg tgccctgtgc cggcgcgtcg tcgtcgctga cgtagcggcc
      301 catcgtcagg gtcagcgcat cgggccgggt gccgcgctcg gccatcagcc gtgtcagccg
      361 cttggcgacg atggtggcga tcttgcggcc caggccgatg tcggtgaaca ggtcttcgcg
      421 cgtcttgttg ccgctccagc gcgtgagctg ctgccagagt gcggcatcgg ccggctgatc
      481 tccgggcggg ccgggcaggt gcaggccttc ggcgcgcatc gcctgcgcga gcatcttctc
      541 gcccaggtcc tgcgactcct cctgctccat gttcttcagg tagtggcgga tcttcgagcg
      601 cgcccggccg gtgcgcacga agttcagcca cgccgggttc ggccgcgcgc cggcggcgct
      661 gacgatctcg accacgtcgc cgctgcgcaa ctcggcgcgc agcgcgaccg gctcgccgtt
      721 gaccttggcc gcgaccgtgt ggtcgccgac atcggtgtgg atcgcgtacg cgaagtcgac
      781 cggcgtcgcg ccgcgcggca gcgcgaggat cttgctcttc ggcgtgaaca cgtagaccgc
      841 gtcggggaac aggtcgatct tgacgtgctc gaggaactcg ttggcgtcgt gggtctcgtc
      901 ctggatgtcg atcagcgact gcagccacat cgcaccgagc cgctgcgcct cctgcacctg
      961 cgcgccgccg ctcttgtaga gccagtgcgc ggcgatgcct ttctcggcga ccgcgtgcat
     1021 ctgctcggtg cggatctgga actcgatcgc cgtgcccagc gggctcacca gcgtcgtgtg
     1081 cagagactgg tagccgttcg ccttcgggat cgcgatgtag tccttgaagc ggccgggcag
     1141 cggcttgaac agctggtgca gcacgccgag cgccatgtag cactccatca gcgtgcccac
     1201 gacgatgcgg aagccgaaga tgtcgttgac ctgcgcgaag gtcaggtgct tctcgcgcat
     1261 cttcttgtag atcgagaaca gcgtcttctc gcggccgtag acctgcaccg gcagcttggc
     1321 ctgcaggaag gcccggtcga cgtcgtgctg gatgcgctcg acgatgtcgc gccggtagcc
     1381 gcgcgccttc agcaccgcct tgctcagggc cgcctggcgc cacggccgca ggtgctggaa
     1441 cgagagctcc tgcagttcgc ggtaggtctg gttcaggccg aggcggtggg cgatcggcgc
     1501 gtagatctcg agcgtttcgc cggcgatgcg gctgcgcttg gcggcggcca tcgcgtccat
     1561 cgtgcgcatg ttgtgcaggc gatcggcgag cttgatgaga atgacgcgca catcgcgcgc
     1621 catcgccagc agcatcttgc ggaacgactc ggcctgcgac tcctcgcggg tcgagaactg
     1681 cagcttgtcg agcttggtca ggccgtcgac caggtcggcg accggcgcgc cgaacttctc
     1741 gatcagctcg atcttggtga cgccgcagtc ttccattgcg tcgtgcatca acgccgccat
     1801 gatggcctgc gcgtcgagct tccattcggc gcacaggccg gcgaccgcga tcgggtgcgt
     1861 gatgtagggc tcgccgctgg cgcggaactg gcccaggtgc gcctcgtcgg cgaagcggta
     1921 ggcctcgcgc acgcgtttga tgtcggcggc gtcgaggtag tcgagcttgt gggtgagggc
     1981 ggcgaacgag gcggccgccg cgtcggcgac ggccggcggc ggcttcgcgg agggcgcgcg
     2041 cttgcccgcg acggcgctcg gggccgggtc tcgcagggcg ttgggggcgc gggcgctcat
     2101 cgcccgaatt taacgtggat tgagccccgt cgtcggtgag gggctacccg acctgggccg
     2161 cctcgcagcg aggcagcggg ggagccgttc ttagaccggg accttgcgca gcatctcgac
     2221 gccgacttcg ccggcagcga tctcgcgcaa cgcggtcacg ccgggcttgt tcttcgtctc
     2281 gatcttcggc gcgtggccct ggctcagcat gcgcgcgcgg taggtcgccg cgagcaccaa
     2341 ctgaaagcgg ttcggaatct tggtgaggca gtcttcgacg gtgatgcggg ccatgggttc
     2401 tttcgcgggt cgggcggtca gctcaggtcg agcgcggcaa agacagcagg cttgcttctt
     2461 tggagggcag cgtacttgag tcgctgcgaa tgaacgatcg ccttcagatc gaagagcgcc
     2521 gtttcaaaca aggcattgat tataacgaag tcgaactcgc gggccttcga gacctcgatg
     2581 cgcgcgttct ccatccgctt ggcgatgacc tcgggccggt cctcgccgcg ccggttcagg
     2641 cgctgctgca gttcgtccca gctcggcggc aggatgaaga tcaggatcgc gttcggaaac
     2701 agctgcttga tctgcagcgc gccctgccag tcgatctcga gcacgacgtc ctgcccgccc
     2761 gtgatgcgcg tctcgatcgc ggcgcgcgag gtgccataga ggtggccgtg cacctgcgcc
     2821 cactcgaaga actcgccgcg ttcgatcatc gcgcggaaag taggctcgtc ggtgaagtgg
     2881 tactcgcggt cctgctgctc ctggccgcgc ggcgcgcgcg tggtgtgcga aaccgcgacg
     2941 accaggtgcg agtcgacctg cagcagcgcg ctcaccaggc tcgatttgcc cgcgccgctc
     3001 ggggcggcga cgacgaagag gttgccgggg tagtccatga tcgcgagagt ctgttgaaag
     3061 ggcgcaggcc aatgcgcgct cgtcgcgcgc aggcggcaag tatcgctcct gcggctcagc
     3121 ctcaggcgag cgccgggccg ccccaagccg cctgagctcc cctcggggga cggcgccgaa
     3181 ggcgaccagg ggtcgtcaga ccacgcgctt gagcttcatc gcgttgggca gcggcaccga
     3241 gcgcttgagc accgtctcgc ccagccacag cgcgctgcgg cgggcgcgct cggcgaccgt
     3301 gggcagcggc atttgctggt agtcgtcgtt gaagacctcg aggctgtagt cgccccggta
     3361 acccagctga tgcagccgcg tcacccagtc ggccacctgc tcgctgtgca cgccttcacc
     3421 cgggaagacg cggaaatgcc gcgcggtgct gatgcgctct tccgccgagc ggacctcctg
     3481 ccacatgaag tcggccagct ggacgaggta gatcttgtac ggatcgagca gctccaggtc
     3541 ttccgcgggg gtcttggtcg cgaaggcgtg gaacgaatcc agggcgatgc cgaggttggg
     3601 catgtcggcg cgagtcacca cctcccaggc ctgcatgaac tcgttgacat ggcggcccca
     3661 gctcagcgct acataggccg ccttcacgcc cagcggcacg gccagcatcg cgagtttgcg
     3721 cagatcgcgg gcggcccgat cgatgtcgcc gtcggcgtgg gccgaggtgg acgagcacac
     3781 cagcagcacc ttggcgccca ggcgcgcgca catctcgagc atcgacttgg cgacatcgat
     3841 cttgtactgg tgcagatggc cgccgaggcc ctcgtagtcg cgcagcacct ggaagccggt
     3901 gattcgcagg ccgctcgagc gcaccgccgc caccgcggcg tcgaggccgt cggggtggcc
     3961 gaccacgtcg cgcgcgccga gcatgatctg cgtgaagcca gcctcgcgag cggccttcag
     4021 cttggcctcc agcgggcctg cgaaggagat cgtgtcgacg ccgaagtcgt cgaagttgcc
     4081 ggcatcgtgc acgggggttc gcagcgcgga tcgcatggct cactccttca cgtgcacgaa
     4141 ctcgaaggac acaccccgca gcacgggctt ggtcagcgcg ccgcgcgcct cggagtgcag
     4201 gcgctcggat tcgtggaact ccacgccgcg ctcgcgcagc agcgacacgg cgcccagcac
     4261 gtcggtcgtg ccgaaggcca cgcgcatgag ctgctcgtcg tcctccacgt cgaggatgcc
     4321 gggttccggc tcgatgagct gcagatagaa gctgccgcaa gggctggcca gcacccggcc
     4381 tttaggcagg atgccgaaag cctgcttcga cgtgaccggc tcgaagccga acagctcgcg
     4441 atagaagtcg gtccagtcct cggtgcggtc gttgcccacg tattgcacga tgccgaacca
     4501 gcgcatgccc gccagcgagg gggaggggta cgggtc
//
LOCUS       DASRDG010000087         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_13326, whole genome shotgun sequence.
ACCESSION   DASRDG010000087 DASRDG010000000
VERSION     DASRDG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13326"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFA35_01440"
     CDS             complement(<1..295)
                     /locus_tag="VFA35_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54861.1"
                     /translation="MLSIVRRFVALFALLGATSVSAQTYTYSIYVDADNNTATGCSVS
                     LPGGNVTGAESVLTATINAGSPPQVAQMTRSSCAGGSFGAPVGFGTAAVGVGAG"
     gene            467..817
                     /locus_tag="VFA35_01445"
     CDS             467..817
                     /locus_tag="VFA35_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZT54862.1"
                     /translation="MTHPSPDLRFRLLLDAEHALGPGKADLLEAIAATGSIAAAGRSM
                     GMSYKRAWQLADELNRSFAEPLIAANKGGQRGGGATLTAAGETVVRIYREIERKAKRA
                     CAAELRALSRLLAD"
     gene            940..1671
                     /gene="modA"
                     /locus_tag="VFA35_01450"
     CDS             940..1671
                     /gene="modA"
                     /locus_tag="VFA35_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT54863.1"
                     /translation="MLGAAVFATAEARADDLTVSAAASLTNAFTDIAKAYETQHPGTH
                     VNLNFGASDVLLKQIEQGAPADVFASADETTMDRAASAGRIDAASRRDFAANTLVLVT
                     PAGSTAPAKLGDLAQDAYKHIAIGNPDSVPAGRYAKQALAEAKLWDVLQPKFVQAQNV
                     RQALDYVARGEAEAGFVYATDAASQAAKVKVALTVPTATPVHYPAAAVGPCKQKPAAC
                     EFVQFLATPASRATLAKYGFSPVNP"
     gene            1677..>2005
                     /locus_tag="VFA35_01455"
     CDS             1677..>2005
                     /locus_tag="VFA35_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HZT54864.1"
                     /translation="MEFLSPLWLTLKVAALATALALILGVAAARALGRKNGLARDTAE
                     SLLTLPLVLPPTVLGYYLLVVLGRNGAIGGWLHDAFGISLIFTWQGATIAAAVAAFPL
                     ILRAARAA"
BASE COUNT          331 a          701 c          649 g          324 t
ORIGIN      
        1 cgcccgcgcc gacgccgacc gcggccgtgc cgaacccgac cggcgcaccg aaactgccgc
       61 ccgcgcagga gctgcgcgtc atctgtgcga cctgcggcgg cgagcccgcg ttgatcgtcg
      121 cggtcagtac cgactccgcc ccggtgacgt tgccgcccgg caggcttacg ctgcagccgg
      181 tcgccgtgtt gttgtcggcg tcgacgtaaa tcgaataggt ataggtctgt gcggatacgg
      241 acgtcgcgcc aagcagcgcg aacagggcga caaatcgccg aacgatggac agcacgtgat
      301 tcccctgaat ccatggtggc tgccgtgggt ggacccgtac ctggcagcag agtgtgtgat
      361 gctacgcaga ttgtatgaaa atttttgaag acccaatcac aaatcgatgc atccgcacga
      421 tgtccgctga cccgttttcc ggtgatttca cgttattgta ttttttatga cccatccatc
      481 gcccgatttg cgcttccgcc tgctgctcga cgccgagcat gcgctcggcc cgggcaaggc
      541 cgacctgctc gaggcaatcg cggcgaccgg ctcgatcgcc gccgcgggcc gttcaatggg
      601 catgagctac aagcgtgcct ggcagctcgc cgacgagctc aaccgcagct tcgccgagcc
      661 gctgatcgcg gcgaacaagg gcggccagcg cggcggcggc gccacgctga ccgcggccgg
      721 cgaaacggtt gtccgcatct atcgcgagat cgaacgcaaa gcgaagcgcg cctgcgcggc
      781 ggaactgcgc gcgctgtcgc gcctgctcgc ggattgacgc tcctacgaca tagcggatgg
      841 cggccttcgt cgcgaggcga tatagtccat aaaccatatc gactggagcc ggccatgccc
      901 cgccaacccc cttcacactc gcgactcgcc tgcacgctga tgctcggtgc cgccgtcttc
      961 gcgactgccg aagcgcgcgc cgacgatctc accgtttcgg ccgcggccag cctgaccaac
     1021 gcgttcaccg acatcgccaa ggcgtatgag acgcagcacc cgggcacgca tgtgaacctc
     1081 aacttcggcg cctccgacgt gctgctcaag cagatcgagc agggtgcgcc ggccgacgtg
     1141 ttcgccagcg ccgacgagac gacgatggac cgcgccgcga gcgccggccg tatcgacgcc
     1201 gcctcgcgcc gcgatttcgc cgcgaatacg ctcgtgctgg tcacgcccgc cggcagcacg
     1261 gcgccggcga agctcggcga tctcgcccag gatgcctaca aacacatcgc gatcggcaac
     1321 cccgattcgg tgccggccgg acgctatgcg aagcaggcgc tggccgaggc gaagctgtgg
     1381 gacgtgctgc aaccgaagtt cgtgcaggcg cagaacgtgc gccaggcgct cgactacgtc
     1441 gcgcgcggcg aggccgaggc cggtttcgtc tatgcgaccg acgcggcgtc gcaggcggca
     1501 aaggtgaaag tggcgctgac cgtgccgacg gcgacgccgg tgcattatcc ggctgccgcg
     1561 gtcggcccct gcaagcagaa accggcagcc tgcgagtttg tgcagttcct ggcgacgccc
     1621 gcgagccgtg cgacactggc aaaatacgga ttcagtccgg tcaatccata acccatttgg
     1681 aattcctctc tccgctctgg ctcacgctca aggtcgccgc gctcgcgacg gcgcttgcgt
     1741 tgatcctcgg cgtcgccgcc gcgcgcgcgc tcggccgcaa aaacggtctg gcgcgcgaca
     1801 cggccgagtc gctgctgacc ctgccgctgg tattgccacc gaccgtgctc ggctactacc
     1861 tgctcgtcgt gctcggccgc aacggcgcga tcggcggatg gctgcacgac gcgttcggca
     1921 tcagcctgat cttcacctgg cagggcgcga cgatcgccgc ggcggtcgcg gcgtttccgc
     1981 tgatcctgcg cgcggcgcgc gcggc
//
LOCUS       DASRDG010000088         4631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_587862, whole genome shotgun sequence.
ACCESSION   DASRDG010000088 DASRDG010000000
VERSION     DASRDG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587862"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFA35_01460"
     CDS             complement(<1..331)
                     /locus_tag="VFA35_01460"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZT54865.1"
                     /translation="MSHEYFNPVRSVYGAGALSSLPDLLAGRRALLVTFPEARALGLV
                     GRIETLLGDRLAGVIDDVRPNPDVAELKALYEGFWRRSDQGGGVDVIVAIGGGSAIDT
                     AKALMVGT"
     gene            complement(340..1581)
                     /gene="phnA"
                     /locus_tag="VFA35_01465"
     CDS             complement(340..1581)
                     /gene="phnA"
                     /locus_tag="VFA35_01465"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HZT54866.1"
                     /translation="MNTRVETIEANGRRYARPQAPVVVVCVDGCEPDYITQAIQAGAA
                     PFLKQMLERGTSLIGDCVVPSFTNPNNVSIVTGVPPAVHGICGNYFYDVANDREVMMN
                     DPAYLRAPTILARFSEAGAKVAAVTAKDKLRTLLGHELKGICFSAEKADQATLAECGI
                     DQVLELVGKPVPSVYSAELSEFIFAAGVKLLQTRRPDLMYLSTTDYVQHKCAPGTPEA
                     NAFYTMLSGYLAQLDALGAVIGLTADHGMSPKTDAAGRPRVLYLQQELDAAFGAGRAR
                     VILPITDPYVVHHGALGSFATVYLPDAATTARAAEALQALPGVDLVLGRAEAARRFEL
                     AADRIGDLVVVSDHLTVLGTSESRHDLSGLTVPLRSHGGISEQKVPLLFNRPLHGIAP
                     GRRLRNFDVFDLALNHVGPPR"
     gene            complement(1581..2399)
                     /gene="phnE"
                     /locus_tag="VFA35_01470"
     CDS             complement(1581..2399)
                     /gene="phnE"
                     /locus_tag="VFA35_01470"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HZT54867.1"
                     /translation="MMNIGTYSLLVAKPTGHLGWWPRAAVGVLTVAVLWWAAKGSQVS
                     FGELAKGLPWIGDFLARMFPPNWAFLDKLVVPAIETVQIAIWGTLLAFILALPVCFLA
                     ARNLTPNLAVFHFTRQVFNVTRGINEIILALIFVAAVGLGPFPGVLALAVHGAGMLGK
                     FFSESIEEIDQGPIEALRSTGAGAIQIIVFGVLPQVITAWIAVLLYRFETNLRQATVL
                     GMVGAGGIGFELVGSMKLFQYQDTATCILVIIVMVMAADTVSTKLRAAIQKGAR"
     gene            complement(2396..3211)
                     /gene="phnC"
                     /locus_tag="VFA35_01475"
     CDS             complement(2396..3211)
                     /gene="phnC"
                     /locus_tag="VFA35_01475"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HZT54868.1"
                     /translation="MIKIRGLTKRYGDFEALKGIDIDVAPGEFLVVLGPSGAGKSTLL
                     RCINRLAEPTRGDIHIDGQLVKPDAASLRKLRRQVAMIFQHYNVVPRLSVQKNVLTGR
                     LGAMPSVLSWLQIFPRQDVEIARECLRRVELEEKAELRTDTLSGGQKQRVGIARALAQ
                     QPKIILADEPVASLDPKTSRTVLQYLKQASRELGISVVCNLHQIEYAREFGERIVGVS
                     AGRVVFEGGPAELSDEVLQRIYPGLDEGEMPKSSPPPRDVPAAVVHNFSLEDA"
     gene            complement(3208..4086)
                     /gene="phnD"
                     /locus_tag="VFA35_01480"
     CDS             complement(3208..4086)
                     /gene="phnD"
                     /locus_tag="VFA35_01480"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT54869.1"
                     /translation="MTLTRRSLIAAAPALLGAPLLGLASPARAADVLTVGLIPSEDSR
                     AMIANSQAMMDMLSKALGMPVKPFVAADYNGVIEALRSKKLDVAYLGPFSYVLGTTVA
                     DIEAFAVAETKKAGRTYYHSLIVAHKDSGIKTVADLKGKTFAFVDPSSTSGHLFPKAG
                     LMKLGFNPDKDFGRVIFSGSHDSNAVAVQNKRVDAVAIADRILDAAIAKGLAKREDLV
                     VVWKSDPIPESPTVWRKDLDPALKKRVQAAFLEVKNIPWSDQGELNGFHPTNDAAYDV
                     IRDTAKVLNLDLRKMK"
     gene            4257..>4631
                     /locus_tag="VFA35_01485"
     CDS             4257..>4631
                     /locus_tag="VFA35_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZT54870.1"
                     /translation="MRLTQLRSFHAVATAGSFTRAAESLHVSQPTVTTQVRQLEDLYK
                     VELFHRTGRRVRPTEIGERLLNLSRQIFSLEADAIQMLGDTGELRSGHLRVAAVGPSH
                     VTKMLAAFNQRYPGVKISVSTGN"
BASE COUNT          757 a         1514 c         1618 g          742 t
ORIGIN      
        1 cggtgccgac catcagcgcc ttggcggtgt cgatcgcgct gccgccgccg atcgcgacga
       61 tcacgtcgac gccgccgccc tggtcggacc ggcgccagaa cccttcatac agcgccttca
      121 gctcggcgac gtcggggttc ggtcggacgt cgtcgatcac gccggcgagc cggtcgccga
      181 gcagcgtctc gatgcggccg acgaggccga gcgcgcgcgc ctcggggaag gtcaccagca
      241 gcgcgcggcg accggccagc aggtcgggca gggacgacag ggccccggcc ccgtacacgc
      301 tgcgaacggg gttgaagtat tcgtgcgaca tggcttcctc tagcggggtg ggccgacgtg
      361 gttcaacgcg aggtcgaaca cgtcgaagtt gcgcagccgc cggccgggcg cgatgccgtg
      421 cagcgggcgg ttgaacagca gcgggacctt ctgctccgag atgccgccgt gagaacgcag
      481 cggcacggtc aggcccgaca ggtcgtgccg cgactcgctc gtgccgagca cggtcaggtg
      541 gtcggagacc acgaccagat cgccgatgcg gtcggcggcc agctcgaagc gacgcgccgc
      601 ctcggcgcgg ccgagcacca gatcgacgcc gggcagggcc tgcaaggcct cggcggcgcg
      661 cgcggtcgtc gcggcgtcgg gcaggtagac ggtggcgaac gagccgagcg cgccgtggtg
      721 cacgacatag gggtcggtga tcggcaggat cacgcgcgcc cggcccgcgc cgaatgcggc
      781 gtcgagttcc tgctggagat agagcacgcg cggccggccg gcggcgtcgg tcttcgggct
      841 catgccgtgg tcggcggtca ggccgatcac cgcgccgagc gcatccagct gggccaggta
      901 gccgctcagc atcgtgtaga acgcgttcgc ttccggcgtg ccgggcgcgc acttgtgctg
      961 cacgtagtcg gtggtcgaca ggtacatcag gtcgggccgc cgcgtctgca gcagcttgac
     1021 cccggcggcg aagatgaact ccgacagctc ggccgagtac accgacggca ccggcttgcc
     1081 gaccagctcg agcacctggt cgataccgca ctcggccagc gtcgcctggt ccgccttctc
     1141 ggccgagaag cagatgccct tcagctcgtg cccgagcagc gtgcgcagct tgtccttggc
     1201 ggtgacggcc gcgaccttcg cgccggcctc ggagaacctg gcgaggatgg tcggcgcgcg
     1261 caggtacgcc ggatcgttca tcatcacttc gcgatcgttg gcaacgtcgt agaagtagtt
     1321 gccgcagatg ccgtgcaccg caggcggcac cccggtgacg atcgacacgt tgttcgggtt
     1381 cgtgaagctg ggcacgacgc agtcgccgat caacgacgtg ccgcgctcga gcatctgctt
     1441 caggaacggc gcggcgccgg cctggatcgc ctgggtgatg tagtcgggct cgcagccgtc
     1501 gacgcagacc accaccaccg gcgcctgcgg ccgggcatag cggcggccgt tggcctcgat
     1561 cgtttcgaca cgggtgttca tcaacgggct cccttctgga tcgcggcacg cagcttggtc
     1621 gagacggtgt cggccgccat caccatcacg atgatcacga ggatgcaggt ggcggtgtcc
     1681 tggtactgga acagcttcat gctgccgacg agctcgaagc cgatgccgcc ggcgccgacc
     1741 atgccgagca cggtggcctg gcgcaggttg gtctcgaagc ggtagagcag gaccgcgatc
     1801 caggccgtga tcacctgcgg cagcacgccg aagacgatga tctggatcgc cccggcgccg
     1861 gtcgagcgca gcgcctcgat cgggccctgg tcgatctcct cgatcgactc cgagaagaac
     1921 ttgccaagca tgccggcgcc gtgcacggcg agcgccagca cgccggggaa gggcccgagg
     1981 ccgaccgcgg cgacgaagat cagcgcgagg atgatctcgt tgatgccgcg ggtgacattg
     2041 aacacctggc gcgtgaaatg gaacacggcc aggttcggcg tcaggttgcg cgcggccagg
     2101 aagcacaccg gcagcgcgag gatgaacgcg agcagcgtgc cccagatcgc gatctggacg
     2161 gtctcgatcg ccggcacgac cagcttgtcg agaaaagccc agttcggcgg gaacatgcgg
     2221 gccaggaagt cgccgatcca cggcaagccc ttggccaatt caccgaagct gacctggctg
     2281 cccttggcgg cccaccagag cacggcgacg gtgagcacgc cgaccgcggc gcgcggccac
     2341 cagccgagat ggcctgtcgg cttggcgacc agcagcgaat aagtaccgat gttcatcatg
     2401 cgtcctcgag cgagaagttg tggaccaccg cggcaggcac gtcgcggggc gggggcgagg
     2461 acttcggcat ctcgccttcg tcgagcccgg ggtagatgcg ctgcagcacc tcgtcgctca
     2521 gctccgccgg gccgccttcg aagacgacac ggccggccga gacgccgacg atgcgctcgc
     2581 cgaactcgcg cgcgtactcg atctggtgca ggttgcagac gaccgagatg ccgagctcgc
     2641 ggctggcctg cttcaaatac tgcagcacgg tgcgcgaggt cttcgggtcg aggctcgcga
     2701 ccggctcgtc ggcgaggatg atcttcggct gctgcgccag cgcacgggcg atgcccacac
     2761 gctgcttctg cccgcccgac agggtgtcgg tgcgcagctc ggccttctct tccagctcga
     2821 cacgccgcag gcattcgcgc gcgatctcga cgtcctggcg cggaaagatc tgcagccacg
     2881 agagcaccga cggcatcgcg ccgagccggc cggtgagcac gttcttttgc accgacaggc
     2941 gcggcaccac gttgtaatgc tgaaagatca tcgcaacctg ccggcgcagc ttgcgcaggc
     3001 tggcggcatc gggcttcacg agctggccgt cgatgtggat gtcgccgcgc gtcggctcgg
     3061 cgagccggtt gatgcaacgc agcagcgtcg acttgccggc gcccgaaggc ccgagcacga
     3121 cgagaaactc gcccggcgcg acgtcgatgt cgatgccctt cagggcctcg aagtcgccgt
     3181 agcgcttggt caggccgcgg atcttgatca tttcatcttc ctcaggtcga ggttgagaac
     3241 cttggcggtg tcgcgaatca cgtcgtaggc cgcgtcgttg gtcgggtgga agccgttgag
     3301 ctcgccctgg tcggaccagg ggatgttctt cacttccagg aacgccgcct ggacccgctt
     3361 cttcagcgcc gggtcgaggt ccttgcgcca gacggtgggc gactcgggga tcgggtcgga
     3421 cttccagacc accaccaggt cctcgcgctt ggccaggccc ttggcgatcg ccgcgtcgag
     3481 gatgcggtcg gcgatcgcca ccgcgtcgac tcgcttgttc tgcaccgcga ccgcgttcga
     3541 gtcgtgcgaa ccggagaaga tgacgcggcc gaagtccttg tcggggttga agccgagctt
     3601 catcagaccg gccttcggga acagatggcc ggaggtcgaa ctcggatcga cgaaggcgaa
     3661 ggtcttgccc ttcaggtcgg ccaccgtctt gatgccgctg tccttgtggg cgacgatcag
     3721 cgagtggtag taggtccggc cggccttctt ggtctcggca acggcaaacg cctcgatgtc
     3781 ggccacggtc gtgcccagca cgtacgagaa cgggccgagg taggccacgt ccagcttctt
     3841 ggaacgcagc gcctcgatca cgccgttgta gtcggccgcg acgaagggct tgaccggcat
     3901 gccgagcgcc ttggacagca tgtccatcat cgcctggctg ttggcgatca tcgcccgcga
     3961 gtcctcggac gggatcaggc cgacggtcag cacgtcggcg gcgcgggccg gcgacgcgag
     4021 gccgagcagc ggtgcgccca gcaaggccgg tgcggcagcg atcagggatc ttcgggtcaa
     4081 ggtcatgttt gtctcctgaa gtgaaggttg gcgggcatcg aggtcgtgcg ccacattcgt
     4141 ttgaagacgc atgacgccac tttcggcgct gcctgagcat ctggcaaatt caatgttttg
     4201 gacgcatgca ttcattcaat ctatagtttg ggtgggttaa cgagcagcgg atcgtcatgc
     4261 gtttgacaca gttgcgttcc ttccatgccg tggcgacggc cggcagcttc acgcgtgccg
     4321 cggagagcct gcatgtcagc cagcccacgg tcacgacgca ggtccggcag ctggaggacc
     4381 tgtacaaggt cgagctcttt catcgcaccg gccggcgcgt gcgacccaca gagatcggcg
     4441 agaggctgct gaacctgtcg cgccagatct tcagcctcga agccgacgcc atccagatgc
     4501 tcggcgacac cggcgagctg cgatcgggtc atctgcgcgt ggcggcggtc ggcccgtcgc
     4561 atgtcacgaa gatgctggcg gccttcaacc agcgctaccc gggcgtcaag atctcggtga
     4621 gcaccggcaa c
//
LOCUS       DASRDG010000089         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1048234, whole genome shotgun sequence.
ACCESSION   DASRDG010000089 DASRDG010000000
VERSION     DASRDG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048234"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFA35_01490"
     CDS             <1..897
                     /locus_tag="VFA35_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54871.1"
                     /translation="VSANRSLAQFDGNGAAQVPITDQKNLVTVQQIGANLAWGGTGLL
                     SVRGDYVHSRVRYSQAQQGSSDSSADSGSIGTYYNLGPDLTAGLALRLTRTEQEAVAG
                     TPGSQTSSSDGRNIDLSMNWRSTAQTGVNARLSWTHQTNAGGGNQDFNGLTGSVAATY
                     APTAKLAFSLGYSRDAGTNGTFFNVPARIGPSGSTPATTVLVQNSQVADSLSLGANYA
                     ATAKIGVTAGYTYRKSRIANLGGGDYDDKLKVASLGANWAIARAWGLGCSLSHEQRDT
                     SATPAFASSANVIGCSAQFTLR"
     gene            894..1916
                     /gene="galE"
                     /locus_tag="VFA35_01495"
     CDS             894..1916
                     /gene="galE"
                     /locus_tag="VFA35_01495"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZT54872.1"
                     /translation="MSCPRVLLTGATGFIGSHTWLSLLAAGFRVVGIDNFTNSSPAVL
                     ERLATLSGDTPEFVQADVCDAAAMAAVFAGGAFDAVVHFAALKAVGESTAKPLAYYAN
                     NVGGLLTLTQAMADRGCKTLVFSSSATVYGRPESLPLREDAALSATNPYGATKLMGEN
                     ILRDVEHADPAWRIALLRYFNPVGAHASGLIGEDPRGIPNNLLPFVAQVAVGRRERLQ
                     VFGNDYDTPDGTGVRDYIHVLDVAEGHVQALRFLLDRQRSITANLGTGRGHSVLEVVR
                     AFERASGVDVPCDFVARRPGDVAASYADVSLAHELLQWRATRDLDTMCADSWRWQRMN
                     PNGLAG"
     gene            1977..>2113
                     /locus_tag="VFA35_01500"
     CDS             1977..>2113
                     /locus_tag="VFA35_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZT54873.1"
                     /translation="MAGGSGTRLWPLSRAQHPKQFLVLQGHRSLFQQATQRLSMLGAP
                     D"
BASE COUNT          343 a          785 c          696 g          289 t
ORIGIN      
        1 gtcagcgcga accgcagcct ggcgcagttc gacggcaacg gcgcagcgca agtcccgatc
       61 accgaccaga agaatttggt gaccgtccag caaatcggcg ccaacctcgc ctggggcggc
      121 accgggctgc tgtcggtgcg cggcgactac gtgcacagcc gcgtgcgcta ctcgcaggcg
      181 cagcagggct cgtccgactc gtcggccgac agcggcagca tcggcaccta ctacaacctc
      241 ggccccgacc tgaccgcggg cctggcgctg cgcctgaccc gcaccgagca ggaagcagtc
      301 gccggaacgc caggcagcca gacgagctcg agcgacggcc gcaacatcga cctctcgatg
      361 aactggcgct ccaccgcgca gaccggcgtg aacgcccggc tgagctggac ccaccagacc
      421 aatgcgggcg gcggcaatca ggacttcaac ggcctgaccg gctcggtcgc ggccacctac
      481 gcaccgaccg ccaagctcgc gttcagcctc gggtacagcc gcgacgccgg caccaacggc
      541 acgttcttca acgtaccggc ccggatcggg ccgagcgggt cgacgccggc gaccaccgtg
      601 ttggtgcaaa acagccaggt cgccgacagc ctgtcgctgg gcgccaacta cgccgcgacc
      661 gccaagatcg gcgtgacggc gggctacacc taccgcaagt cgcgcatcgc caacctgggc
      721 ggcggcgact acgacgacaa gctgaaggtc gcctcgctcg gcgcgaattg ggcgatcgcg
      781 cgcgcctggg ggctcggctg cagcctctcg cacgagcagc gtgacacgag cgccacgccc
      841 gccttcgcct ccagcgccaa cgtgatcggc tgctcggccc agttcacgct gcgatgagct
      901 gtccgcgcgt cctgctgacc ggcgcgaccg gcttcatcgg ctcgcacacc tggctgagcc
      961 tgctggccgc cgggttccgt gtcgtcggca tcgacaactt caccaacagt tcgcccgcgg
     1021 tgctcgagcg cctggccacg ctcagcggcg atacgcccga attcgtgcag gccgacgtgt
     1081 gcgacgcggc ggcgatggcg gctgttttcg ccggcggggc cttcgacgcg gtcgtgcact
     1141 tcgcggcact gaaggcggtc ggcgaaagca ccgccaagcc gcttgcctac tatgccaaca
     1201 acgtcggcgg cctgttgacg ctgacccagg cgatggcaga tcgcggctgc aagacgctgg
     1261 tgttcagctc cagcgccacc gtctacggca ggcccgaatc gctgccgctg cgcgaggacg
     1321 ccgcgttgtc ggcgaccaac ccgtacggcg cgaccaagct gatgggcgag aacatcctgc
     1381 gcgatgtcga acacgccgat ccggcgtggc ggatcgcgct gctgcgctac ttcaacccgg
     1441 ttggcgcgca cgcgagcggc ctgatcggcg aagacccgcg cggcatcccg aacaacctgc
     1501 tgcccttcgt cgcccaggtg gcggtgggcc ggcgcgagcg cctgcaggtc ttcggcaacg
     1561 actacgacac gcccgacggc accggcgtgc gcgactacat ccacgtgctc gacgtcgccg
     1621 agggccacgt gcaggccttg cgcttcctgc tcgaccggca gcgctcgatc accgccaacc
     1681 tcggcaccgg gcgcgggcac agcgtgctcg aggtggtgcg cgcgttcgag cgcgccagcg
     1741 gcgtcgacgt gccctgcgat ttcgtcgcgc gccgcccggg cgatgtggcg gcgagctacg
     1801 ccgacgtatc gctcgcccac gaactgctgc agtggcgcgc gacccgcgac ctcgacacga
     1861 tgtgcgccga cagctggcgc tggcagcgca tgaacccgaa cggactcgcc ggctgatgcc
     1921 ggccgcgcac gagcgaccct gacctcatga cccccatccc cgttcaaccc gtcatcatgg
     1981 ccggcggcag cggcacgcgg ctgtggccgc tctcgcgcgc acagcatccg aagcagttcc
     2041 tcgtgctgca gggccatcga agcctgttcc agcaagccac gcaacggctt tccatgctgg
     2101 gtgcgccgga cat
//
LOCUS       DASRDG010000090         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_818301, whole genome shotgun sequence.
ACCESSION   DASRDG010000090 DASRDG010000000
VERSION     DASRDG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818301"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /gene="tadA"
                     /locus_tag="VFA35_01505"
     CDS             <1..439
                     /gene="tadA"
                     /locus_tag="VFA35_01505"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HZT54874.1"
                     /translation="EHDPTAHAEIVALRHAAQLLSNYRLPECELYVTLEPCAMCAMAL
                     MHARVKRVVFGARDPKTGAAGSVVDLFAQEQLNHHTAVEGGVLADACGAVLRDFFAER
                     RKQYRERRRQRHASAAEAPLPGGEPEIPAGEATEMLIAPPSSP"
     gene            448..1410
                     /locus_tag="VFA35_01510"
     CDS             448..1410
                     /locus_tag="VFA35_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HZT54875.1"
                     /translation="MTLLTLFSPAGVLVKSAPLTLAVKRLAALGFEVRIDAAARARHQ
                     RFAGDDDTRLAALHRIAHEAPGIALATRGGYGLTRLLDRIDWPLIAHSIERGTRWVGQ
                     SDMTTLQLGLLAQPKLGAKAVTWAGPLAVDDFGRAEDAGGVDEITRDCFLEAMSGALE
                     AVGFRTEAGFDGIEARGTLWGGNLCMVNSLLGTKYFPRIKGGVLFLEDVNEHPYRVER
                     NLLQLHQAGVLDAQKAVVLGAFSAWRKAPQDRGFSLKTVVAHLRSLTKTPILTGLPFG
                     HVPTKLTLPVGARVTLLVDGRNVLIGWEHRHAGDHDRGHDHAHA"
     gene            1403..>2078
                     /locus_tag="VFA35_01515"
     CDS             1403..>2078
                     /locus_tag="VFA35_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT54876.1"
                     /translation="MPDRRRALGCVLAAAVALGLAACDNSPNPKGSAATNTLFVAFQE
                     RSPRHLDPTASYAVNEVPYMYAVYEPPYAYHYLKRPYLVVPKAAEALAEPRYLDKNGK
                     PLPADAPGELVAESVYDVPIKHGMRYAPHPAFAKDANGAYLYHALSADETRDKHTPFD
                     FAQIGTREVVADDFVYALERHATPRIETPIFGIFSEYVIGLKEYGALIRAEDRQLRQG
                     SDPAALD"
BASE COUNT          329 a          748 c          711 g          290 t
ORIGIN      
        1 cgaacacgac ccgaccgcgc acgccgagat cgtcgcgctg cggcacgcgg cgcagttgct
       61 gtcgaactac cgcttgcccg agtgcgagct gtacgtgacg ctcgaaccct gcgcgatgtg
      121 cgcgatggcg ctgatgcatg caagggtcaa gcgtgtcgtg ttcggcgcgc gcgaccccaa
      181 gaccggcgcg gccggctcgg tcgtcgacct gttcgcgcaa gagcagctga accaccacac
      241 cgcggtcgaa ggcggtgtgc ttgccgacgc gtgcggcgcc gtgctgcgcg actttttcgc
      301 cgagcgccgc aagcagtatc gagaacggcg gcggcaacgg catgcgagcg ccgccgaggc
      361 gccgctgccg ggcggcgaac ccgagattcc ggccggcgaa gccaccgaga tgctgatcgc
      421 gccgccttca tcaccatgag cacccgaatg accttgctga ccctgttttc gcccgccggc
      481 gtgctcgtga aatccgcgcc gctgacgctc gccgtcaagc ggctcgcggc gctcggtttc
      541 gaggtacgaa tcgacgcagc agcgcgcgca cgccaccagc gcttcgccgg tgacgacgac
      601 acgcgcctgg ccgcgctgca ccgcatcgcg cacgaagcgc ccggcatcgc gctggccaca
      661 cgcggcggct acggcctgac gcggctgctc gaccgcatcg actggccgct gatcgcgcac
      721 agcatcgagc gcggcacgcg ctgggtcggc cagagcgaca tgacgacctt gcagctcggc
      781 ctgctcgcgc aacccaagct gggcgcgaaa gcggtgacct gggccggccc gctcgcggtc
      841 gacgacttcg gccgtgcgga agatgccggc ggcgtcgacg agatcacccg cgactgcttt
      901 ctcgaagcga tgagcggcgc gctcgaggcg gtgggctttc gcaccgaggc tggcttcgac
      961 gggatcgagg cgcgcggcac gctgtggggc ggcaacctgt gcatggtgaa ctcgctgctc
     1021 ggcaccaagt actttccgcg catcaagggc ggcgtgctgt tcctcgaaga cgtcaacgag
     1081 cacccgtacc gggtcgagcg caacctgctg cagctgcacc aggccggcgt gctcgacgcg
     1141 cagaaggcgg tcgtgctcgg cgcgttcagc gcctggcgca aggcgcctca ggaccgcggc
     1201 ttctcgctga agacggtggt cgcgcacctg cggtcgctga cgaagacgcc gatcctgacc
     1261 ggcctgccgt tcggccatgt gccgaccaag ctgacgctgc cggtcggcgc gcgcgtcacg
     1321 ctgctggtgg acgggcgcaa tgtgctgatc ggctgggagc accgccacgc cggcgatcac
     1381 gaccgcgggc acgaccacgc ccatgcctga tcgccgccgc gcgctcggct gcgtgctggc
     1441 cgcggccgtg gcactcgggc tggcggcctg cgacaacagc ccgaacccga agggcagcgc
     1501 ggcgaccaac accttgttcg tcgcgttcca ggagcgctcg ccgcgccacc tcgacccgac
     1561 cgcgtcgtac gcggtgaacg aggtgccgta catgtacgcg gtctacgagc cgccctatgc
     1621 gtaccactac ctgaagcggc cttacctcgt ggtgccgaag gccgccgagg cgctcgccga
     1681 gccccggtac ctcgacaaaa acggcaaacc gctgccggcc gacgcacctg gcgagcttgt
     1741 cgccgagagc gtctacgacg tccccatcaa gcacggcatg cgctacgcgc cgcacccggc
     1801 gttcgcgaag gatgcgaacg gtgcctacct gtaccacgcg ctgtcggcgg acgagacgcg
     1861 cgacaagcac acgccgttcg acttcgcgca gatcggcacg cgcgaggtcg tcgccgatga
     1921 cttcgtctac gcgctcgagc gccacgcgac gccgcgcatc gagacgccga tcttcggcat
     1981 cttctcggag tacgtgatcg ggctgaagga gtacggcgcg ctgatccgcg ccgaggaccg
     2041 ccagctgcgg cagggcagcg acccggccgc gctcgaca
//
LOCUS       DASRDG010000091         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_369960, whole genome shotgun sequence.
ACCESSION   DASRDG010000091 DASRDG010000000
VERSION     DASRDG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369960"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VFA35_01520"
     CDS             complement(<1..1496)
                     /locus_tag="VFA35_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent methanol/ethanol family
                     dehydrogenase"
                     /protein_id="HZT54877.1"
                     /translation="MRLKLLSVLIPLALGSITAQSAVTEKMIEDSAKSGTEVLSWGLG
                     TQGQRYSALKQVNTANVAKLAPAWAFSFGGEKQRGQESQPLIHNGKMFVTASYSRIFA
                     LDAATGKKLWKYEHRLPEGIMPCCDVVNRGAALFDNLVIFATLDAQIVALDQATGDVV
                     WKEKIDDYAAGYSSTAAPIIANGLLLTGVSGGEFGVVGRVEARDPKTGKMVWVRPTVE
                     GHMGYTYDAAGNKKENGVSGTTGKTWQGDLWKTGGAATWLGGSYDAKTGLAYFGTGNP
                     APWNSHLRPGDNLFSCSTVAIDVKTGQIKWSYQGTPNDGWDFDGVNEFVTFDMDGKRV
                     GGKADRNGFFYVNDASTGQLLNAFPFVKKITWATGIDLKTGRPNFVPENRPGDPTASA
                     DGKKGNAIFSAPSFLGGKNQMPMAYSPDTKLFYVPANEWGMEIWNEPITYKKGAAYLG
                     AGFTIKTINDDYIGALRAVDPKTGKIVWEVKNNAPLWGGVLTTAGNLV"
     gene            complement(1715..2680)
                     /locus_tag="VFA35_01525"
     CDS             complement(1715..2680)
                     /locus_tag="VFA35_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HZT54878.1"
                     /translation="MSAPARLLGWAVLLVVLCALPALALAQRADPATLDYQLRARELA
                     GGVYVVEGANADFSVANGCNIINTGFIATGAGVIVINTGPSRRYGEQLRALIARTTPE
                     PVIRVVHLNLHPDYFLGNQAFADVPRLATPATRAGMQRDAKGYEDNLYRLCGEWLKGT
                     EALLPDTDAKPGVERVGAHELEWVELHGHTDSDLVLIDRSRGVVFAGGLVFARRVPTT
                     PHAQVDAWRASLRTLAAWPPAQLVPSHGRVADGWAGIVLTQRYLGWLDSAFRDAAARG
                     LEMNEVLRMPVPDEFRGWAGFASEYVRNVANLYPRYEAAVLERAR"
     gene            complement(2677..>2872)
                     /locus_tag="VFA35_01530"
     CDS             complement(2677..>2872)
                     /locus_tag="VFA35_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HZT54879.1"
                     /translation="GGIPAFYVNRLSLRDGERELLRLNAFEPLSENPVFSFDFAGMPH
                     GKLQLVGTDNNGNRIAGAIE"
BASE COUNT          405 a          964 c         1006 g          497 t
ORIGIN      
        1 aacacgaggt tgccggcggt cgtcagcacg ccaccccaca gcggcgcgtt gttcttcact
       61 tcccagacga tcttgccggt cttcgggtcc acggctcgca gcgcgccgat gtagtcgtcg
      121 ttgatcgtct tgatcgtgaa gccggcgccg aggtaggccg cgcccttctt gtaggtgatc
      181 ggctcgttcc agatctccat gccccattcg ttggcgggca cgtagaacag cttggtgtcg
      241 ggcgagtagg ccatcggcat ctggttcttg ccgccgagga agctcggcgc cgagaagatc
      301 gcgttgccct tcttgccgtc ggcgctcgcg gtcgggtcgc cggggcggtt ctcgggcacg
      361 aagttcgggc ggccggtctt caggtcgatg ccggtggccc aggtgatctt cttgacgaac
      421 gggaaggcgt tcagcaactg gccggtgctc gcgtcgttga cgtagaagaa gccgttgcgg
      481 tcggccttgc cgccgacgcg cttgccgtcc atgtcgaagg tgacgaactc gttgacgccg
      541 tcgaagtccc agccgtcgtt cggcgtgccc tggtagctcc acttgatctg gccggtcttc
      601 acgtcgatcg cgaccgtcga gcacgagaac aggttgtcgc cggggcgcag gtggctgttc
      661 cacggcgccg ggttgccggt gccgaaatag gccaggccgg tcttcgcgtc gtagctgccg
      721 ccgagccagg tggcggcgcc gccggtcttc cagaggtcgc cctgccaggt cttgccggtg
      781 gtgcccgaga cgccgttctc cttcttgttg ccggcggcgt cgtaggtgta gcccatgtgg
      841 ccttcgacgg tcgggcgcac ccagaccatc ttgccggtct tcgggtcgcg cgcttcgacg
      901 cggccgacca cgccgaactc gccgccggac acgccggtca gcagcaggcc gttcgcgatg
      961 atcggcgcgg cggtcgatga atagccggcg gcgtagtcgt cgatcttctc cttccagacc
     1021 acatcgccgg tggcctggtc gagcgcgacg atctgcgcgt cgagcgtcgc gaagatcacg
     1081 aggttgtcga acagcgcggc gccgcggttg accacgtcgc agcagggcat gatgccttcg
     1141 ggcaggcggt gctcgtactt ccagagcttc ttgccggtcg cggcatcgag cgcgaagatg
     1201 cgcgagtacg acgcggtcac gaacatcttg ccgttgtgga tcagcggctg cgactcctgg
     1261 ccgcgctgct tctcgccgcc gaacgagaac gcccacgccg gcgcgagctt ggcgacgttc
     1321 gcggtgttga cctgcttcag cgccgagtag cgctggcctt gcgtgcccag gccccaggac
     1381 agaacctcgg tgccggattt cgccgagtcc tcgatcatct tctcggtcac cgcggattgc
     1441 gcggtgatcg aacccagggc aagcggtatc agaacggaca gcagcttcaa gcgcatgaat
     1501 tgtctcctcg gtggtggatt tcgggccgcg tgtcgcgcgg cctcccggtg acaagggggc
     1561 aacaaccatg ccatcggcgc gggcgcgcgg atggagcaat cagcccaccg tccggacgcg
     1621 ctttcgaccc gatcggaccg ccggtggctg cgggtttcta cgcgcgttgc tacatcctcg
     1681 aacaccggcg tgtgacatcc cggcgcgctg cggttcagcg cgcacgttcg agcaccgccg
     1741 cctcgtagcg cgggtacagg ttggcgacgt tgcgcacgta ctcgctcgcg aagccggccc
     1801 agccgcggaa ctcgtcgggc accggcatgc gcagcacctc gttcatctcg aggccgcgcg
     1861 ccgcggcgtc gcgaaatgcg ctgtcgagcc acccgagata gcgctgcgtc agcacgatgc
     1921 cggcccagcc gtcggcgacg cggccgtggc tgggcacgag ctgcgccggc ggccacgcgg
     1981 ccagcgtgcg caggctggcg cgccatgcgt cgacctgcgc gtgcggcgtg gtcggcacgc
     2041 ggcgcgcgaa caccagcccg cctgcgaaga caacgccgcg gctgcggtcg atcagcacca
     2101 ggtcgctgtc ggtgtggccg tgcagctcga cccactcgag ctcgtgcgcg ccgacgcgtt
     2161 cgacgccggg cttcgcgtcg gtgtcgggca gcagcgcctc ggtgcctttg agccactcgc
     2221 cgcacaggcg gtacaggttg tcttcatagc ccttggcgtc gcgctgcatg cccgcgcggg
     2281 tggcgggggt ggcgagccgc ggcacgtcgg cgaaggcctg gttgccgagg aagtaatcgg
     2341 ggtgcaggtt caggtgaacg acgcggatca ccggctcggg cgtcgtgcgt gcgatcagcg
     2401 cgcgcagctg ctcgccgtag cgccgcgacg ggccggtgtt gatcacgatc acgccggcac
     2461 cggtcgcgat gaagccggtg ttgatgatgt tgcagccgtt ggcgacgctg aagtcggcgt
     2521 tcgcgccctc gaccacgtag acgccaccgg ccagctcgcg cgcgcgcagc tgatagtcga
     2581 gggtcgccgg atcggcccgc tgcgccagcg ccagtgccgg cagcgcacac agcacgacca
     2641 gcagcaccgc ccagccgagc aggcgtgccg gggcgctcat tcgatcgccc ccgcgatccg
     2701 gttgccgttg ttgtccgtgc ccaccagttg cagcttgccg tgcggcatgc cggcgaagtc
     2761 gaagctgaac accgggttct cgctgagcgg ctcgaacgca ttcaggcgca gcagctcgcg
     2821 ctcgccatcg cgcagcgaca ggcggttcac ataaaaggcc gggatgccgc cg
//
LOCUS       DASRDG010000092         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_369996, whole genome shotgun sequence.
ACCESSION   DASRDG010000092 DASRDG010000000
VERSION     DASRDG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369996"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFA35_01535"
     CDS             <1..1029
                     /locus_tag="VFA35_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methylcrotonyl-CoA carboxylase"
                     /protein_id="HZT54880.1"
                     /translation="EADLAEAIAAGRREAKAAFGDDKMIVEKVVSPARHIEIQLLGDK
                     HGNVVSLHERECSVQRRHQKVLEESPSPAVDADLRARMGAAAVKLAKAVGYDNAGTAE
                     FLVDSHGHFYFLEVNARLQVEHPVTELVTGVDLVHLQVRVAAGEKLDDLLRGRDLRPR
                     GHALEARICAEAPEQGFLPAAGLLRLVREPEGPGIRVDSGVYSGWDVPVFYDSLLAKL
                     IVWAPDRATACARMTQALRAAVYLGIPTNVDFLRRVVDDPDFRAGMLTTDFLKHKPGL
                     AVGTPLPPDDVVLAAAVLCQAVAPRAVTQGNSGGSGATVAGDGRAARKSTAVWHELSG
                     LRLFGGDA"
     gene            1026..1535
                     /locus_tag="VFA35_01540"
     CDS             1026..1535
                     /locus_tag="VFA35_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZT54881.1"
                     /translation="MSVQRTYVRDGQAVAVAAEPVGGDHWRVRVGDKVYEFRVAALGD
                     GGVRLVPVGAEAEPACVAYGVPAGKAFMVRVRGRTHTLSAPEARRGGGSGVADGAVRA
                     PMTGTVTRILCREGDAVDADQTLAVLTAMKMEHKLSAGVAGVVRKIAAAEGGTVEQGS
                     VLVQVEPAP"
     gene            1532..2071
                     /locus_tag="VFA35_01545"
     CDS             1532..2071
                     /locus_tag="VFA35_01545"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HZT54882.1"
                     /translation="MTNPAMTTDPRYPIGKAELRGRLDDRARATAIAQIAALPAAMHD
                     AVRGLDQTQLDTPYRDGGWSVRKVVHHVADSHVNAYVRCKLVATESDPPLKGYDENAW
                     STLADANASVGSSLLLLTALHERWAEFLRSLPAAAFARTGLHSERGKMTLDDLTALYA
                     WHGRHHAAHVTTLRAAKGW"
     gene            complement(2096..>3022)
                     /locus_tag="VFA35_01550"
     CDS             complement(2096..>3022)
                     /locus_tag="VFA35_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54883.1"
                     /translation="PRTPGSDVCGLDLDDSANELWVIDSTGGITRCSNACPPVLINTT
                     FLTPGLISHIPTGISIDELRGLVFISATDFGSPQGNGRIYVAPLSSPGSWFQFTQVVD
                     CITTASHRITGLAVDAGNSALYWTDGRNTYKTTYTYTGTPTPGSVVITPQSCCIGLAV
                     GADPLIDLSIRWGGATSSGLPCANGTCPSCPMLHTLRNAPLLGTTLQLGLDLAPVGVP
                     TWCLINFGSCGTGSLLPPLCGPVLVPLNAALVAMGINTTTGSSGCTGTTTFFLFLPPI
                     PALAGTPLASQCVSLCPPTGTTMSNCLSWVLQ"
BASE COUNT          425 a         1054 c         1150 g          393 t
ORIGIN      
        1 gaggcggacc tcgccgaggc gatcgccgcg gggcggcgcg aggcgaaggc cgcgttcggc
       61 gacgacaaga tgatcgtcga gaaggtcgtc tcgccggcgc gccacatcga gatccagctg
      121 ctcggcgaca agcacggcaa cgtcgtgtcg ctgcacgagc gcgagtgctc cgtgcagcgc
      181 cgccaccaga aggtgctcga ggagtcgccg tcgccggccg tcgacgcgga cctgcgcgcg
      241 cgcatgggcg ctgccgccgt caagctcgcg aaggccgtcg gctacgacaa cgcgggcacc
      301 gccgagttcc tggtcgactc gcacggccac ttctacttcc tcgaggtgaa cgcgcggctg
      361 caggtcgagc acccggtgac cgagctggtg accggcgtcg acctcgtgca cctgcaggtg
      421 cgcgtcgccg ccggcgagaa gctcgacgat ctgctgcgcg gccgcgacct gcgcccgcgc
      481 ggccacgcgc tcgaggcgcg catctgcgcc gaggcgccgg agcagggttt cctgcccgcg
      541 gcgggcctgc tgcggctggt gcgcgagccc gaagggcccg gcatccgcgt cgactccggc
      601 gtctactcgg gctgggacgt gccggtgttc tacgactcgc tgctggccaa gctgatcgtc
      661 tgggcgcccg accgcgcgac ggcctgcgcg cgcatgacgc aggcgctgcg cgcggccgtc
      721 tacctcggca tcccgaccaa cgtcgacttc ctgcgccgcg tcgtcgacga tcccgacttc
      781 cgtgccggca tgctgacgac cgacttcctg aagcacaaac ccgggctcgc ggtcggcacg
      841 ccgctgccgc ccgacgacgt cgtgctcgca gccgcggtgc tgtgccaggc ggtggcgccg
      901 cgcgcggtga cgcagggcaa cagcggcggc agcggcgcga ccgtcgccgg cgacggccgc
      961 gcggcgcgca agtcgaccgc ggtgtggcac gaactgtcgg gcctgcgcct gttcggcggt
     1021 gacgcgtgag cgtgcagcgg acctacgtgc gcgacggcca agcggtcgcg gtcgcggccg
     1081 agccggtcgg cggcgaccac tggcgcgtgc gcgtcggcga caaggtctac gagttccgcg
     1141 tcgccgcgct cggcgacggc ggcgtgcggc tggtgccggt cggcgccgag gcggagccgg
     1201 cgtgcgtcgc ctacggcgtg ccggcgggca aggcgttcat ggtgcgcgtc cgcggccgca
     1261 cgcacacgct gtcggcgccc gaggcgcggc gcggcggcgg cagcggcgtc gccgacggcg
     1321 cggtccgcgc gccgatgacc ggcacggtga ccaggatcct ctgccgcgaa ggcgacgcgg
     1381 tcgacgccga ccagacgctc gccgtgctga cggcgatgaa gatggagcac aagctcagcg
     1441 ccggcgtcgc cggggtcgtg cgcaagatcg cggcggccga gggcggcacg gtcgagcagg
     1501 gctcggtgct cgtgcaggtg gagccagcac catgacgaac ccagccatga cgaccgaccc
     1561 gcgctatccg atcggcaagg ccgagctgcg cggccgtctc gacgaccgcg cccgcgcgac
     1621 cgcgatcgcg cagatcgcgg cgctgcccgc ggcgatgcac gacgccgtcc gcggcctcga
     1681 ccagacgcag ctcgatacgc cgtaccgcga cggcggctgg agcgtgcgca aggtcgtgca
     1741 ccatgtcgcc gacagccacg tcaacgccta cgtgcgctgc aagctggtgg cgaccgagag
     1801 cgatccgccg ctcaagggct acgacgagaa cgcgtggtcg acgctcgccg acgcgaacgc
     1861 gtcggtcggt tcgtcgctgc tgctgctcac ggcgctgcac gagcgctggg ccgagttcct
     1921 gcgttcgctg cccgccgcgg cgttcgcgcg cacgggcctg cacagcgaac gcggcaagat
     1981 gacgctcgac gacctcacag cactctacgc ctggcacggc cgccaccacg cagcgcacgt
     2041 cacgacgctg cgcgcagcca agggctggta ggcgcgcagc gctgctgctg cgctgctact
     2101 gcaacaccca cgacaggcag ttgctcatcg tcgtgcccgt cggcgggcac agcgagacgc
     2161 actgcgaggc gagcggcgtg ccggcgagcg cggggatcgg cggcaggaac aggaagaacg
     2221 tcgtcgtgcc ggtgcagccg ctcgaaccgg tcgtcgtgtt gatgcccatg gccaccagcg
     2281 ccgcgttgag cggcaccagc accgggccgc acaggggcgg gaggaggctg ccggtgccgc
     2341 acgagccgaa gttgatcagg caccaggtgg gcacgccgac cggcgccagg tcgaggccga
     2401 gctgcagcgt cgtgccgagc agcggcgcgt tgcgcagcgt gtgcagcatc gggcagctcg
     2461 ggcaggtgcc gttcgcgcac ggcagcccgc tcgacgtcgc gccgccccag cggatgctga
     2521 ggtcgatcag cgggtcggcg cccacggcga ggccgatgca gcacgactgc ggcgtgatga
     2581 cgaccgagcc cggcgtcggc gtgccggtgt acgtgtaggt ggtcttgtac gtgttgcgcc
     2641 cgtcggtcca gtagagcgcc gagttgccgg cgtcgacggc gaggccggtg atgcggtgcg
     2701 acgccgtcgt gatgcagtcg acgacctgcg tgaactggaa ccagctgccg gggctgctca
     2761 gcggcgccac gtagatgcgg ccgttgccct gcggcgagcc gaagtccgtg gcgctgatga
     2821 acacgagccc gcgcagttcg tcgatgctga tgccggtcgg gatgtgcgag atcagccctg
     2881 gcgtgaggaa cgtcgtgttg atgagcaccg gcgggcaggc gttgctgcag cgcgtgatgc
     2941 cgccggtgct gtcgatgacc cacagttcgt tggcactgtc gtcgaggtcg aggccgcaga
     3001 cgtcggagcc gggagtgcgc gg
//
LOCUS       DASRDG010000093         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_818701, whole genome shotgun sequence.
ACCESSION   DASRDG010000093 DASRDG010000000
VERSION     DASRDG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818701"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VFA35_01555"
     CDS             complement(<1..167)
                     /locus_tag="VFA35_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT54884.1"
                     /translation="MTVAALPAAPDAPLPASLRWALMFGNFVIGCGVMCVAGTLNDMA
                     ESLTVSVALAG"
     gene            complement(164..1171)
                     /locus_tag="VFA35_01560"
     CDS             complement(164..1171)
                     /locus_tag="VFA35_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HZT54885.1"
                     /translation="MNIIILDDYQDAVRKLKCASVMEALNAKVFTNTVKGIGQLSVRL
                     RDAEVLVPIRERTQFPKQLLEKLPKLKLISQTGKAGSHIDIETCTKMGIAVAEGVGSP
                     VAPAELTWALIMAATRRLPQYIGNLKHGAWQQSGLKAASMPANFGLGTVLKGRTLGIW
                     GYGKIGRLVAGYGKAFGMHVTVWGSAAARERADQDGFVAAPSCEAFFEVCDVLSLHLR
                     LHDSTRGIVKPDALARMKPTALLVNTSRAELIEEGALVSALNRGRPGMAAVDVFESEP
                     ILQGHPLLRLENAVCTPHIGYVEQDSYELYFKAAFDNVVNFINGTPTNILNPEALRVL
                     R"
     gene            1290..1802
                     /locus_tag="VFA35_01565"
     CDS             1290..1802
                     /locus_tag="VFA35_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HZT54886.1"
                     /translation="MSITMHSASVPIFTSMLENMSAWLDKAEAYAAAKKFEPAVLLAA
                     RLAPDMLPLTTQVQIACDTAKFLVARLAGVDAPKFDDTETTLAELRARIAATIEFVKS
                     VPAAQIDGTEDKEVTLPRRTGPIVMKGEAYLKHFALPNFFFHATTTYALLRHNGVELG
                     KLDFLGAPKQ"
     gene            complement(1817..2125)
                     /locus_tag="VFA35_01570"
     CDS             complement(1817..2125)
                     /locus_tag="VFA35_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZT54887.1"
                     /translation="MSTSTIKAPKAASAAECGALEQLPNIGPSLAADLRSIGIERPSD
                     LRGKDAFVLYQKLCAVTGQRQDPCVLDTFMAAVDFMRGAPAAPWWHYTPQRKALFGQV
                     "
     gene            complement(2131..2823)
                     /gene="lexA"
                     /locus_tag="VFA35_01575"
     CDS             complement(2131..2823)
                     /gene="lexA"
                     /locus_tag="VFA35_01575"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZT54888.1"
                     /translation="MYEPPKLTDRQQQILDLVQSAIERTGAPPTRAEIASELGFKSAN
                     AAEEHLQALARKGVIELVGGTSRGIRLRSETLRQLNQQRGKQFSLPLPSLAQLTLPLI
                     GRVAAGSPILAQEHVDHTYLMEASMFPRRPDYLLKVRGMSMRDAGIIDGDLLAVQRTK
                     DAKNGQIVVARLGDEVTVKRFRRTRHSIELIPENPDFQTIVVPTSGSDAGELQFELEG
                     LAVGLIRNNMMM"
     gene            2938..>3786
                     /locus_tag="VFA35_01580"
     CDS             2938..>3786
                     /locus_tag="VFA35_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZT54889.1"
                     /translation="MQNKVPVEASIVVVLGTGGTIAGTAASAADNIGYTAARLGAAQL
                     VAAIPALAGAPIEVEQVAQLDSKDMGFAVWRALAAAVDRHLARPEVGGVVVTHGTDTL
                     EETAYFLQRVLAPAKPVVLAAAMRPATSAQPDGPQNLLDAMVVAREPGARGVVAVLAG
                     TVHSALDVRKVHTYRLDAFGSGDAGALGQVEQGRLRRHRAWPEGEAIGLARLPHDIAQ
                     WPVVEIVTSHSEARGTLATALRDAGVQGIVVAATGNGTVHHALEAALLDAQAAGVAVL
                     RATRCLD"
BASE COUNT          561 a         1289 c         1327 g          609 t
ORIGIN      
        1 tgcccggcca gagcgaccga gaccgtcagc gactcggcca tgtcgttcaa cgtgccggcg
       61 acgcacatca cgccgcagcc gatcacgaag ttgccgaaca tcagcgccca cctcaggctg
      121 gcgggcagtg gcgcgtccgg cgccgccggc agtgcggcca cggtcatcga agcactcgca
      181 aggcctcggg gttcaggatg ttcgtcggcg tgccgttgat gaagttcacg acattgtcga
      241 aggcggcctt gaagtagagc tcgtagctgt cctgctcgac gtagccgatg tgcggcgtgc
      301 acacagcgtt ctcgagccgc agcagcgggt ggccctgcag gatcggttcg ctctcgaaca
      361 cgtcgaccgc cgccatgccg ggccggccgc ggttcagtgc cgagaccagc gcgccttctt
      421 cgatcagctc ggcgcgcgag gtgttgacga gcagcgccgt cggcttcatg cgcgcgagcg
      481 cgtcgggctt gacgatgccg cgggtgctgt cgtgcaggcg caggtgcagc gagagcacgt
      541 cgcagacctc gaagaaggcc tcgcagctcg gcgcggcgac gaagccgtcc tgatcggcac
      601 gctcgcgcgc agcggcgctg ccccacacgg tgacgtgcat gccgaaggcc ttgccgtagc
      661 ccgcgacgag ccggccgatc ttgccgtaac cccagatgcc cagcgtccgg cccttgagca
      721 ccgtgccgag gccgaagttc gccggcatcg aggcggcctt cagccccgac tgctgccatg
      781 cgccgtgctt caggttgccg atgtactgcg gcaggcggcg cgtcgccgcc atgatcaagg
      841 cccaggtcag ctccgccggt gcgaccggcg agcccacgcc ctcggcgacc gcgatgccca
      901 tcttcgtgca ggtctcgatg tcgatgtgcg agccggcctt gccggtctgc gagatcagct
      961 tcagcttcgg cagcttctcg agcagttgct tcgggaactg ggtgcgctcg cggatcggca
     1021 ccagcacctc ggcgtcgcgc aggcggacag acagctggcc gatgcccttg acggtgttgg
     1081 tgaagacctt ggcgttcagc gcttccatca cgctggcgca cttgagtttg cgtaccgcgt
     1141 cctggtagtc gtcgaggatg atgatgttca cggatgtggc gggtcgctcg gtgctgcctg
     1201 caggctcggc acccgattgt gcacggctcg tgcaggcgct gtagcatccc cgctcccgac
     1261 cccgtcgacc gcccggaggc aagcacacca tgtcgatcac catgcactcg gccagcgtgc
     1321 cgatcttcac ctcgatgctc gagaacatga gcgcctggct cgacaaggcc gaggcttacg
     1381 cggcggcgaa gaagttcgag cccgccgtgc tgctggcggc ccggctcgca cccgacatgc
     1441 tgccgctgac gacccaggtc cagatcgcct gcgacaccgc gaagttcttg gtcgcgcgcc
     1501 tggccggcgt ggacgcgccg aagttcgacg acaccgaaac caccctggcc gaattgcgtg
     1561 cgcgcattgc tgcgacgatc gagttcgtca agtcggtgcc ggccgcgcag atcgacggca
     1621 ccgaggacaa ggaggtgacg ctgccgcgcc gcaccggccc gatcgtgatg aagggcgagg
     1681 cctatctgaa gcacttcgcg ctgcccaact tcttcttcca cgccaccacc acctacgcgc
     1741 tgctgcgcca caacggcgtc gagctcggca agctggactt tctgggcgcg ccgaagcaat
     1801 agcttcgacc tgcgcttcag acctgcccga acagcgcctt gcgctgcggc gtgtagtgcc
     1861 accacggcgc ggccggcgcg ccgcgcatga agtcgaccgc ggccatgaag gtgtcgagca
     1921 cgcacgggtc ctggcgctgg ccggtgacgg cgcagagctt ctggtagagc acgaaggcat
     1981 ccttgccgcg caggtcggac gggcgctcga tgccgatcga ccgcagatcg gccgcgagcg
     2041 acgggccgat gttgggcagt tgctcgaggg ccccgcactc ggccgcgctc gcggccttcg
     2101 gggccttgat ggtcgaggtg ctcattgcga tcacatcatc atgttgttgc ggatcaggcc
     2161 gaccgccagg ccttcgagct cgaactgcaa ctcgcccgca tcggaaccgc tggtgggcac
     2221 gacgatggtc tggaaatcgg ggttctcggg gatcagctcg atgctgtggc gggtgcggcg
     2281 aaagcgcttg accgtgacct cgtcgccgag ccgcgcgacc acgatctggc cgttcttggc
     2341 gtccttggtg cgctgcaccg cgagcaggtc gccgtcgatg atgccggcgt cgcgcatgct
     2401 catgccgcgc accttcagca ggtagtcggg ccggcgcggg aacatgctgg cttccatcag
     2461 gtaggtgtgg tcgacatgct cttgcgccag gatcgggctg cccgcggcga cacgcccgat
     2521 cagcggcagc gtgagctgcg ccaggctcgg cagcggcagg ctgaactgct tgccgcgctg
     2581 ctggttgagc tggcgcagtg tctcggaacg cagccggatg ccgcgcgagg tgccgccgac
     2641 cagttcgatc acgcccttgc gtgccagtgc ctgcaagtgc tcctcggccg cgttggccga
     2701 cttgaagccg agctcgctgg cgatctcggc ccgcgtgggc ggggcgccgg tgcgctcgat
     2761 cgcgctttgc acgagatcga ggatctgctg ttgccgatcg gtgagcttgg gcggttcgta
     2821 cacaggtttt ccttgatgat gggcgggtgc gccgcggccc ggttcgattc gactcgatgt
     2881 cgaggatcgg gctgacgatt catccagtac ctgtattttc atccagcttc gcaaaaaatg
     2941 caaaacaaag tgcctgtcga agcgtccatc gtcgtcgtgc tcggcaccgg cggcacgatc
     3001 gccggcaccg cggcgagcgc cgccgacaac atcggctaca cggccgcacg gctcggcgcg
     3061 gcgcagctgg tcgcggccat tccggccttg gccggcgcgc cgatcgaggt cgaacaagtg
     3121 gcgcagctcg acagcaagga catgggcttc gcggtctggc gggcgctggc ggcggccgtc
     3181 gaccggcacc tcgcgcggcc cgaggtcggc ggggtcgtcg tcacgcacgg caccgacacg
     3241 ctggaggaaa ccgcctactt cctgcagcgc gtgctcgcgc cggccaagcc ggtcgtgctg
     3301 gccgcggcga tgcgcccggc cacctccgcg cagcccgacg gcccgcagaa cctgctcgat
     3361 gcgatggtcg tcgcgcgcga acccggcgcg cgcggcgtgg tcgcggtgct cgccggcacg
     3421 gtgcacagcg cgctcgatgt gcgcaaggtg cacacctacc ggctcgacgc gttcggctcg
     3481 ggcgatgcgg gcgcgctcgg ccaggtcgag caggggcgcc tgcgccggca tcgcgcctgg
     3541 cccgagggcg aggcgatcgg actggcgcgc ttgccgcacg acatcgcgca atggccggtg
     3601 gtcgagatcg tcacgagcca cagcgaggcg cgcggcacgc tggcgacggc gctgcgcgac
     3661 gcgggcgtgc agggcatcgt cgtcgcagcc accggcaacg gcacggtgca ccacgcgctg
     3721 gaggcggcgc tgctcgacgc gcaggccgcc ggtgtggcgg tactgcgcgc gacgcgctgc
     3781 ctggac
//
LOCUS       DASRDG010000094         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1396278, whole genome shotgun sequence.
ACCESSION   DASRDG010000094 DASRDG010000000
VERSION     DASRDG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396278"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VFA35_01585"
     CDS             complement(<1..1261)
                     /locus_tag="VFA35_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZT54890.1"
                     /translation="MMKHKNPRRVPVAGALAALSALVLTLGGCMSLAPTYERPAAPVA
                     PAYAPELMPAGSTAGAAAADIEWQRFFADPRLARLIALALQNNRDLRVAVLNIEAARA
                     NYQIRRADQWPTLGAGVSASREAGTNGGLVNVYAAGLQITGFELDFFGRVRSLSEAAL
                     AQYLATDAARKTTQIALIAGVATTYVSLLADDELLRVTRDALKAREESFGLTRLRFDN
                     GVSSEVDLRQAEQLLETARVALAQTQRQKALDENALTLLVGQPLPGDLPAPLPLGAQP
                     GVADLPAGLPSDLLARRPDVLAAEQQLRAANANIGAARAAFFPRIALTASAGTASTEL
                     SGLFKSGSWALTGSASLLQTIFDAGRNQANLQIAKVNKDIAIAQYEKAIQTAFREVSD
                     SLAGRATLGEQLRAQAAVANAAQVSFNL"
     gene            complement(1258..>2693)
                     /locus_tag="VFA35_01590"
     CDS             complement(1258..>2693)
                     /locus_tag="VFA35_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT54891.1"
                     /translation="PPGATQGRTLEVMQQVEGFMLKQPEVQSMVSVLGFSFSGTGQNA
                     ALAFVPLKDWSERHGAGHSAQALAGRAFGALMGVRDAFIFALSPPPIPELGSATGFTF
                     RLQDRGGNGHAALLGARNQLLGMAAQSKILAGVRPDGLEDAPQLQLDIDRDKAQALGV
                     DFSAVNAALSTSLGSAYVNDFPNAGRLQRVVVQADAPERMQPDDLLRINVLNSKGQPV
                     PLAAFATTHWVNGPVQTVRYNGYPTMRISGDAAPGQSTGQAMLEMERLAKQLPAGFGF
                     EWTGQSREERIAGSQAYILYGFSILAVFLCLAALYESWTIPLAVIMVVPLGVLGVVLG
                     TMFRGLANDVYFQVGLITIIGLSAKNAILIIEFAKDLEAEGKPPLEAALAAAHLRFRP
                     IIMTSLAFILGTLPLVVASGAGSASQRAIGTAVIGGMITAVVLAVLFVPAFFVVVRAV
                     FKKSKVKPHRGAPLGHDTPPAPAQEGA"
BASE COUNT          379 a          957 c          972 g          385 t
ORIGIN      
        1 ccaggttgaa gctcacctgc gcggcattcg ccaccgcggc ctgcgcgcgc aactgctcgc
       61 cgagcgtggc gcggccggcg agcgaatccg agacctcgcg gaacgcggtc tggatcgcct
      121 tctcgtactg cgcgatcgcg atgtccttgt tgaccttggc gatctgcaga ttggcctggt
      181 tgcgcccggc gtcgaagatc gtctgcagca ggctcgcgct gccggtcagc gcccaactgc
      241 cgctcttgaa cagccccgag agctcggtgc tcgcggtgcc ggcgctcgcc gtcagcgcga
      301 tgcgcgggaa gaacgcggcg cgcgccgcgc cgatgttcgc gttcgcagcg cgcagttgct
      361 gctcggcggc gagcacgtcg ggccggcgcg ccagcaggtc cgacggcagg cccgcgggca
      421 ggtcggccac gcctggctgt gcgccgagcg gcagcggcgc gggcaggtcg cccggcagcg
      481 gttggccgac caacagggtc agcgcgttct cgtcgagcgc cttctggcgc tgggtctgcg
      541 cgagtgcgac gcgcgcggtc tcgagcagct gctcggcctg gcgcagatcg acctcgctcg
      601 agacgccgtt gtcgaagcgc agtcgcgtca ggccgaacga ctcctcgcgc gccttcaatg
      661 cgtcgcgcgt gacgcgcagc agctcgtcgt cggccagcag gctgacgtag gtggtcgcca
      721 cacccgcgat cagcgcgatc tgcgtcgtct tgcgtgccgc gtcggtggcc aggtattgcg
      781 ccagcgccgc ttcgctgagc gagcgcacac ggccgaagaa gtcgagctcg aagccggtga
      841 tctgcagccc ggcggcgtag acgttgacca gcccaccgtt cgtgcccgcc tcgcgcgacg
      901 ccgagacgcc ggcaccgagc gtgggccatt ggtcggcgcg ccggatctgg tagttggcgc
      961 gcgccgcctc gatgttgagg accgcgacgc gcaggtcgcg gttgttctgc agcgcgagcg
     1021 cgatcaggcg cgcgagccgc gggtcggcga agaagcgctg ccattcgatg tccgccgcgg
     1081 cggcgccggc ggtcgatccg gccggcatca gctccggcgc gtaagccggc gcgaccggcg
     1141 ccgcggggcg ctcgtaggtc ggtgccaagc tcatgcagcc gccgagtgtc agcaccagcg
     1201 ccgaaagcgc ggccagcgcg ccggcaaccg gcacgcggcg ggggttcttg tgcttcatca
     1261 tgcgccctcc tgcgccgggg ccggcggcgt gtcgtggccc agcggcgcgc cgcggtgcgg
     1321 cttgaccttg ctcttcttga agacggcgcg cacgacgacg aagaacgccg gcacgaacag
     1381 caccgcgagc acgaccgcgg tgatcatgcc gccgatcacc gcggtgccga tcgcgcgctg
     1441 gctcgccgag ccggcgccgc tcgcgaccac cagcggcaag gtgccgagga tgaacgccag
     1501 cgaggtcatg atgatcggcc gaaagcgcag gtgcgccgcg gcgagcgcgg cttcgagcgg
     1561 gggcttgccc tcggcctcga ggtctttcgc gaactcgatg atcaggatcg cgttcttcgc
     1621 cgacaggccg atgatcgtga tcagcccgac ctggaagtac acgtcgttcg cgaggccgcg
     1681 gaacatcgtg ccgagcacca cgccgagcac gcccagcggc accaccatga tcaccgcgag
     1741 cgggatcgtc cagctctcgt acagcgccgc caggcacaaa aagaccgcca ggatcgagaa
     1801 gccgtacagg atgtaggcct gcgaccccgc gatgcgctct tcgcgcgact ggccggtcca
     1861 ctcgaagccg aagccggccg gcagctgctt cgcgagccgc tccatctcga gcatcgcctg
     1921 gccggtcgac tggcccggcg ccgcgtcgcc cgagatccgc atcgtcgggt agccgttgta
     1981 gcgcaccgtc tgcaccgggc cgttgaccca gtgcgtcgtc gcgaaggccg ccagcggcac
     2041 cggctggccc ttgctgttga gcacgttgat gcgcaacagg tcgtcgggct gcatgcgctc
     2101 gggcgcatcg gcctgcacga ccacgcgctg cagccggccg gcgttcggga agtcgttgac
     2161 gtaagccgag ccgagcgagg tcgacagcgc cgcgttgacc gcgctgaagt cgacgccgag
     2221 cgcctgcgcc ttgtcgcggt cgatgtcgag ctgcagctgc ggcgcgtctt cgaggccgtc
     2281 ggggcgcacg cccgcgagaa tcttgctctg cgccgccatg ccgagcagct ggttgcgcgc
     2341 gccgagcagc gcggcgtggc cgttgccgcc gcggtcctgc agccggaagg tgaaaccggt
     2401 cgcgctgccg agctcgggaa tcggcggcgg gctgagcgcg aagatgaacg cgtcgcgcac
     2461 gcccatcagc gcgccgaaag cccggcccgc gagcgcctgc gccgagtgcc cggcgccgtg
     2521 gcgctcgctc cagtccttca gcggcacgaa cgcgagcgcg gcgttctggc ccgtgcccga
     2581 gaagctgaag ccgagcacgc tgaccatgct ctgcacctcg ggctgcttga gcatgaagcc
     2641 ctcgacctgc tgcatcacct ccagcgtgcg gccctgcgtg gcgccgggcg gca
//
LOCUS       DASRDG010000095         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_15291, whole genome shotgun sequence.
ACCESSION   DASRDG010000095 DASRDG010000000
VERSION     DASRDG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15291"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="VFA35_01595"
     CDS             complement(<1..1320)
                     /locus_tag="VFA35_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZT54892.1"
                     /translation="MNRSAVLALLLALAAAMLAWLLRPAVSPPSDGAASARHDPSAAP
                     AVAAADRARDERTPLPTTAPNANDAASAPFATIRGRCVADEDGRPLAGCEVRCSTRDP
                     AAVTAADGRFEVHVTNSNNPSLRIIAAERTPRTATWDKPLPPHAVEDLGDVRMQRGFE
                     VRGRVVDESGVGLADCNLFVYGIEVSARAGIAQQETAGAATAGDGSFTFGSLLPAGEW
                     ETQVGTRVAYRDAPFRFHVDASAGCPPLWIVVTRGKHLRAHVVDEDGKPVALVRFHTE
                     TGDSWARSDDDGVIDFWSTRSVAGSTRILLDNDDWPDPEFVPPTVAWGSEDARLVLHR
                     GGSLPIAVVDDSGVPVEEFAVHIAHEPLADRWEPARETGRHAGGLLTIPRVHRGPNLL
                     RVVPRAPELLPGEVTRVAVTGHEREPVRVQLERLRVCPVQVVTQAGQP"
     gene            complement(1317..>2398)
                     /locus_tag="VFA35_01600"
     CDS             complement(1317..>2398)
                     /locus_tag="VFA35_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54893.1"
                     /translation="RRPEPAKFFARFLALTEAHRQFVQQRLGARAARDDDAVRAALAA
                     LPGDELRAALLPRELRDLAFGYREEVNDLHLPHVRELPQVLPTLFAAAAARARDAGFD
                     GVELHYAHAYTMASFLSRTNVRSDGYGGSPQARLRLPLEVFAAVRARVGRDFCVGVRY
                     LGDEAIAGGSDVDDAVAYGVAFARAGVDYLSISKGGKFDDAKQPHVGEAVYPYTGHSG
                     HECMPTVRSDERGPFGRNVHLAAAIRSAVRAAGCTTPVVTSGGICSFEQAEAILQRGE
                     ADAVAAARQSLADPDWFLKVRLGRGAEVRRCVYTNYCEGLDQKHKQVTCQLWDRDFAD
                     GAAADGAVPRSSDGKRRLLPPEWRP"
BASE COUNT          302 a          883 c          893 g          320 t
ORIGIN      
        1 gggctgcccc gcctgcgtga ccacctgcac cgggcacacg cgcaggcgtt cgagttggac
       61 gcgcaccggc tcgcgttcgt ggccggtcac cgcgaccctc gtcacttcgc caggcagtag
      121 ctcgggcgcg cgcggcacca cgcgcagcag gttggggcct cggtgcacgc ggggaattgt
      181 cagcaggcca cccgcatgtc tgcctgtttc gcgcgccggc tcccagcggt cggccagcgg
      241 ctcgtgagcg atgtgcaccg cgaactcctc gaccggcaca cctgagtcgt cgacgacggc
      301 gatcggcagc gaaccgccgc gatgcagcac gagccgcgcg tcctcgctgc cccaggccac
      361 cgtcggcggc acgaactcgg gatcgggcca gtcgtcgttg tcgagcagga tgcgcgtgct
      421 gccggcgacg gacctcgtgc tccagaagtc gatcacgccg tcgtcgtccg accttgccca
      481 ggaatcgccg gtctcggtgt ggaaccgcac cagcgcgacc ggcttgccgt cctcgtcgac
      541 gacgtgcgcg cggaggtgct tgccgcgcgt gacgacgatc cacagcggcg gacagccggc
      601 gctcgcgtcg acgtggaacc ggaacggcgc gtcgcggtac gcgacccgtg tgcccacctg
      661 tgtctcccac tcgcccgcgg gcagcaggct gccgaaggtg aacgagccgt cgccggccgt
      721 cgcggccccg gccgtctcct gttgcgcgat gccggcgcgg gcggacacct cgatgccgta
      781 gacgaacagg ttgcagtcgg cgagtccgac gccggactcg tcgacgacgc ggccgcgcac
      841 ctcgaagccg cgctgcatgc gcacgtcgcc gaggtcctcg acggcgtggg gtggcagcgg
      901 cttgtcccat gtcgcggtcc gaggcgtgcg ctccgcagcg atgatgcgga ggctggggtt
      961 gttgctgttc gtcacgtgaa cctcgaagcg tccatcggcg gcggtgacgg ccgcagggtc
     1021 ccgtgtgctg cacctgacct cgcaacccgc gagcggccgg ccgtcctcgt cggcgacgca
     1081 gcggccgcgg atcgtcgcga acggcgcgga agcggcatcg ttcgcgttgg gtgcggtcgt
     1141 cggcagcggc gtgcgctcgt cgcgtgcgcg atcggccgcc gcgacggcgg gcgccgcgct
     1201 cgggtcgtgc cgcgcgctcg cggcaccgtc gctcggcggc gaaacggcgg ggcgcagcag
     1261 ccacgcgagc atcgcggcgg cgagggcgag cagcagcgcg agcaccgcgg accggttcac
     1321 ggccgccact ccggcggcag cagtcgccgc ttgccgtcgc tcgaccgcgg caccgcgccg
     1381 tccgcggcgg caccgtccgc gaagtcgcgg tcccacagct ggcaggtgac ctgcttgtgc
     1441 ttctggtcga ggccttcgca gtagttcgtg tagacgcagc ggcggacttc cgcgccgcgg
     1501 cccagccgca ccttcaggaa ccagtcgggg tcggcgaggc tctggcgcgc ggcggcgacg
     1561 gcgtcggcct cgccgcgctg caggatcgcc tccgcctgct cgaacgagca gatgccgccc
     1621 gaggtcacca ccggcgtcgt gcagccggcg gcgcgcaccg cgctgcggat cgcggcggcg
     1681 aggtgcacgt tgcgcccgaa cggcccgcgt tcgtcgctgc gcacggtcgg catgcactcg
     1741 tggcccgagt ggccggtgta cggatacacc gcctcgccga cgtgcggctg cttcgcatcg
     1801 tcgaacttgc cgcccttgct gatcgacagg tagtcgacgc cggcgcgcgc gaacgcgacg
     1861 ccgtaggcca ccgcgtcgtc gacgtcgctg ccgccggcga tcgcctcgtc gccgaggtag
     1921 cgcacgccga cgcagaagtc gcggccgacg cgcgcgcgca ccgcggcgaa cacctcgagc
     1981 ggcagccgca gccgcgcctg cggcgaaccg ccgtagccgt cgctgcgcac gttggtgcgc
     2041 gacaggaacg acgccatcgt gtaggcgtgc gcgtagtgca gttcgacgcc gtcgaagccg
     2101 gcgtcgcgcg cgcgcgcggc ggcggcggcg aacagggtgg gcagcacctg cggcagctcg
     2161 cgcacgtgcg gcaggtgcag gtcgttgacc tcttcgcggt agccgaacgc gaggtcgcgc
     2221 agctcgcgcg gcagcagcgc cgctcgcagc tcgtccccgg gcagcgccgc gagcgccgcg
     2281 cgcaccgcgt cgtcgtcgcg cgccgctcgc gcgccgagcc gttgctgcac gaactggcga
     2341 tgtgcctcgg tgagcgcgag gaagcgggcg aagaacttcg ccggctcggg ccgccgcc
//
LOCUS       DASRDG010000096         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1523437, whole genome shotgun sequence.
ACCESSION   DASRDG010000096 DASRDG010000000
VERSION     DASRDG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523437"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFA35_01605"
     CDS             complement(<1..496)
                     /locus_tag="VFA35_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT54894.1"
                     /translation="MNLSAPFVKRPIGTVLLTVGIALAGIAGFFVLPVSPLPQVDFPV
                     ISVSASIPGASPETMATSVATPLERHLGTIAGVTEMTSNSQVGSARISLQFELSRDID
                     GAAREVQAAINASRVDLPATLRSNPTYRKANPSDAPCIILALTSKARTPQQIYDAVSN
                     IVQQK"
     gene            complement(1035..>3829)
                     /locus_tag="VFA35_01610"
     CDS             complement(1035..>3829)
                     /locus_tag="VFA35_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT54895.1"
                     /translation="LPADLPAPPVYAKVNPADAPVLTLAITSDTLPLTEVQNLVNTRL
                     AQKISQVSGVGLVSLSGGQRPAVRIQADTRALAANGIGLDTVRSAITAANSNGAKGSF
                     DGPKRAYSINANDQLLTVDDYKNLIVSYKNGAPIRMLDVARVVDSAENTQLGAWAGFG
                     CNHSGAASQAAGDCLHPAIILNVQRQPGANVIATVDAIKKRLPELQAGLPAALRIEVL
                     SDRTTGIRASVEHVQMELVLAVVLVVLVIFAFLGSLRATVIASIAVPISLVGSFGLMY
                     LLGYSLNNLSLMSLTIATGFVVDDAIVMIENIARYIEHGERPMAAALKGATQIGFTII
                     SLTVSLIAVLIPLLFMGDVVGRLFREFAVTLAITILISAVVSLTLVPMMSARWLAAHG
                     KDEKPKAGIAARAQAFFAGVMTRYDRGLHWVIAHQALTLIVALITLVLTVVLYLTIPK
                     GLFPTQDTGQLQARVEAAQDVSYERMAQLQQAAAREILKDPAVQSLSSFVGVDAANNT
                     MLHTGSMLINLVQSHGNQQDVITRLRERVHEVAGVTLYLQPTQDLTIDAETGPTEFRV
                     SLEGVDSGTITQWMNKLVAQLQTVKQVRNVSSDAGAQGLAAYINVNRDTASRLSITAS
                     SVDDALYSAFGQRIVSTIFTETNQYRVILEARPGVLTNAQSLGDLNLITGGGAATPLS
                     AIASIVEQPAPLQITHVAQYPASTLNFDTAPGVSLGTAVDAIRQAAKDIGLPSTVTMT
                     FLGASGAYEKSLSNQLWLILAAVVCVYIVLGVLYESYVHPLTILSTLPSAGVGALLAL
                     IVTGNDLGVIGIIGMILLIGIVKKNAIMMIDFAIDAEREQGMSARDAIHQAALLRFRP
                     ILMTTLAALFAAVPLMLSWGDGAELRRPLGLAIFGGLIVSQLLTLFTTPVIYLAFDRL
                     GRRMRGPRSAASETAA"
BASE COUNT          593 a         1297 c         1326 g          613 t
ORIGIN      
        1 ccttctgctg cacgatgttg gacaccgcgt cgtagatctg ctgcggcgtg cgggccttcg
       61 aggtcagcgc caggatgatg cagggcgcgt ccgacggatt ggccttgcgg taggtcgggt
      121 tcgagcgcag cgtcgccggc aggtcgacgc gcgatgcgtt gatcgccgcc tgcacctcgc
      181 gcgccgcgcc gtcgatgtcg cgcgagagct cgaactgcag gctgatgcgc gcactgccga
      241 cctggctgtt cgacgtcatc tcggtgacgc cggcgatcgt gccgaggtgg cgctcgagcg
      301 gcgtggccac actcgtcgcc atcgtctcgg ggctcgcgcc ggggatgctc gcgctgaccg
      361 agatgaccgg gaagtcgacc tgcggcagcg gtgacaccgg cagcacgaag aagccggcga
      421 tgccggcgag cgcgatgcct accgtcagca gcacggtgcc gatcgggcgt ttgacgaagg
      481 gggcggagag attcacctct cgaacctccg ggcggcaaag gcgcgttcgg gctgggcgcg
      541 tcgcttcgcc tcggcggtcg ccgcttcgcg acgactgctc tgcggtgctc ggtcacgtgg
      601 cccgtcgcga aactcgctcc gttcgctcac gctcactttg ctcagacacc gcgacgagtc
      661 agtcaacgaa gcgcgctgcg cgcgcggcca cgcgccctgc gctcctcgac gcctcccacg
      721 aagcgacgcg cccagcccga acgcgccttt gctccaccat cgtggcaccc gcgagtcgct
      781 ccaatggtgg acttcgcggc aggcggcacc cggcaggggc gatttctgtg gcggcgagga
      841 gcgcagcttc gaggtcggcg cgcgcagcgc gcttcgttga ctgactcgcc gcggctgttt
      901 gagcgcagtg agcgaagcga acggagcgag ttcggcggcg cgacctcgaa gcgagcaccg
      961 cagcgcagtc gtcgcgaagc ggcgaccgcc acagcatgag cccctgccgg gtgccgcctg
     1021 ccgcgacgcg ccactcatgc agccgtctcc gatgcagccg agcgtggtcc gcgcatcctc
     1081 cgccccaagc gatcaaacgc cagatagatc accggcgtcg tgaacagcgt caacaactga
     1141 ctcacgatca acccgccgaa aatcgccagc cccaaaggcc gccgcaactc ggccccatca
     1201 ccccagctga gcatcagcgg caccgcggcg aacaaggccg ccagcgtcgt catcagaatc
     1261 ggtcggaagc gcagcagcgc ggcctggtgg atcgcatccc gcgcgctcat gccctgctcg
     1321 cgctcggcgt cgatcgcgaa gtcgatcatc atgatcgcgt tcttcttgac gatgccgatc
     1381 agcaggatca tgccgatgat gccgatcacg ccgaggtcgt tgccggtgac gatcagcgcg
     1441 agcagcgcgc cgacgccggc cgacggcagc gtcgagagga tcgtcagcgg atgcacatag
     1501 ctttcgtaca gaacgccgag cacgatgtag acgcagacca ccgcggcgag gatcagccag
     1561 agctggttgc tgagcgactt ctcgtacgcc cccgacgcgc cgaggaaggt catcgtcacg
     1621 gtgctcggca ggccgatgtc tttcgcggcc tggcggatcg cgtcgaccgc cgtaccgagc
     1681 gacacgcccg gcgcggtgtc gaagttcagc gtgctcgccg ggtactgcgc gacgtgcgtg
     1741 atctgcaacg gcgcgggctg ctcgacgatg ctggcgatcg ccgacagcgg cgtggcggcg
     1801 ccgccaccgg tgatcaggtt caggtcgccg agcgactgcg cgttcgtcag cacgccgggg
     1861 cgcgcttcga ggatcacgcg gtactggttc gtctcggtga agatcgtcga gacgatgcgc
     1921 tggccgaacg cgctgtagag cgcgtcgtcg accgagctcg cggtgatcga caggcgcgag
     1981 gcggtgtcgc ggttcacgtt gatgtaggcg gccaggccct gcgcgccggc gtcgctgctg
     2041 acgttgcgca cctgcttgac ggtctgcagc tgcgcgacca gcttgttcat ccactgcgtg
     2101 atcgtgccgc tgtcgacgcc ttcgagcgag acgcggaact cggtcgggcc ggtctcggcg
     2161 tcgatcgtca ggtcttgcgt cggctgcagg tagagcgtga cgccggcgac ctcgtgcacg
     2221 cgctcgcgca gccgcgtgat cacgtcctgc tggttgccgt gactctggac caggttgatc
     2281 agcatgctgc cggtgtgcag catcgtgttg ttcgccgcgt cgacaccgac gaacgagctc
     2341 aggctctgga ccgccgggtc tttcaggatc tcgcgcgccg cggcctgctg cagctgcgcc
     2401 atgcgctcgt acgagacgtc ttgcgcggcc tcgacacggg cctgcagttg gccggtgtcc
     2461 tgcgtcggga acaggccctt cgggatcgtc aggtagagca cgaccgtcag caccagcgtg
     2521 atcagcgcga cgatcagcgt cagcgcctgg tgcgcgatga cccagtgcag gccgcggtcg
     2581 tagcgcgtca tcacgccggc gaagaaggcc tgcgcgcgcg cggcgatccc ggccttgggt
     2641 ttctcgtctt tgccgtgcgc cgcgagccag cgcgccgaca tcatcggcac caaggtcaac
     2701 gagacgacgg ccgagatcag gatcgtgatc gccagcgtca ccgcgaactc gcggaacagc
     2761 cgcccgacca cgtcgcccat gaacagcagc gggatcagca ccgcgatcag cgacaccgtc
     2821 agcgaaatga tcgtgaagcc gatctgggtc gcgcccttca gcgccgcggc catcgggcgc
     2881 tcgccgtgct cgatgtagcg cgcgatgttc tcgatcatca cgatcgcgtc gtcgacgacg
     2941 aagccggtcg cgatcgtcag cgacatcagg ctcaggttgt tcaggctgta gccgagcagg
     3001 tacatcaggc cgaagctgcc gaccagcgag atcggcaccg cgatgctcgc gatcacggtc
     3061 gcgcgcagcg agccgaggaa cgcgaagatc accagcacga cgagcacgac cgccagcacc
     3121 agctccatct gcacgtgctc gaccgacgcg cggatgccgg tcgtgcggtc gctcaacacc
     3181 tcgatgcgca gcgcggccgg caggccggcc tgcagctcgg gcaggcgctt cttgatcgcg
     3241 tcgacggtcg cgatcacgtt cgcgccgggc tggcgctgca cgttcaggat gatcgccggg
     3301 tgcaggcagt cgcccgcggc ttgcgatgcc gcgccactgt ggttgcaacc gaagccggcc
     3361 cacgcgccga gctgcgtgtt ctcggcgctg tcgacgacgc gtgcgacatc gagcatgcgg
     3421 atcggcgcgc cgttcttgta cgagacgatc aggttcttgt agtcgtcgac cgtcagcaac
     3481 tggtcgttcg cgttgatcga gtacgcgcgc ttcgggccgt cgaagctgcc cttcgcgccg
     3541 ttcgagttgg cggccgtgat cgcgctgcgc acggtgtcca ggccgatacc gttcgcggcc
     3601 agcgcgcgcg tgtcggcctg gatgcgcacg gccggccgct gcccgccgct gagcgagacg
     3661 aggccgacgc ccgagacctg gctgatcttc tgcgcgagcc gcgtgttgac gaggttctgc
     3721 acctcggtca gcggcagcgt gtcggaggtg atcgcgagtg tgagcaccgg cgcgtcggcc
     3781 gggttcacct tggcgtagac cggcggcgcg ggcaggtcgg ccggcagca
//
LOCUS       DASRDG010000097         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_15737, whole genome shotgun sequence.
ACCESSION   DASRDG010000097 DASRDG010000000
VERSION     DASRDG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15737"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFA35_01615"
     CDS             <1..945
                     /locus_tag="VFA35_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZT54896.1"
                     /translation="RLPTEGSADEFDQLAGTVNGMLDRIEQQTAAVRVTFNSAAHDLR
                     APLFRVRARLESVLQSGTDNGATSRALQATLEDLERVQRTLATLLQIAQAEAGGGGMP
                     PEPLDLAQMASELGELYALEARERGLQLRVHAPQPAIMQGHRQLLAQLLTNLLENAIK
                     YVPAGGTIDLDVALNAARIELTVSDDGPGIPDLAHGSMLLPFRRLERDHGAPGSGLGL
                     SLVNAVVRLHQGQLELRSMSPGLCVSCTFAAASGAAAVTGAAAPAGVAAAAAGATAAA
                     PAAVARASPFANAPSAPIVDRTESTGGFVITGVVKPKG"
     gene            982..1854
                     /locus_tag="VFA35_01620"
     CDS             982..1854
                     /locus_tag="VFA35_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HZT54897.1"
                     /translation="MLVASAFCAAATPPVYEVRDDDGRLLSLRAPPQRIVSLAPGATA
                     MLFAAGGGTRVVGTPEFSVEPEAARAIPRIGDSHGFDLERILALHPDLLVAWSGGASA
                     AQLLPFERAALPVYHHRVTRLDELPRSIERLGVLLHTEPEARRAAAALAARINAIHAH
                     YRRDNPPRLLLQVWDRPVYTLGGGQLTADAAEACGYRNVYAELHDAAPAVSLESIVAR
                     DPDVILVLAADEGTAQQWLDHWKSLPALRAVRNHRVVSFVDARLSRLGPETVDATEAL
                     CEKLAGVAPPASTP"
BASE COUNT          295 a          738 c          719 g          289 t
ORIGIN      
        1 cggctgccca cggaaggcag cgccgacgag ttcgaccagc tggccggcac ggtcaacggc
       61 atgctcgacc gcatcgagca gcaaaccgcc gccgtgcgcg tgaccttcaa cagcgcggcg
      121 cacgacctgc gcgcgccgct gttccgcgtg cgtgcacgcc tcgagagcgt gctgcaatcc
      181 gggacggaca acggtgcgac cagccgcgca ctgcaggcga cgctcgagga cctcgagcgc
      241 gtgcagcgca cgctggcgac gctgctgcag atcgcgcaag cggaagccgg cggcggcggc
      301 atgccaccgg agccactgga cctggcgcaa atggcgagcg aactcggtga gctctacgcg
      361 ctcgaggcgc gcgaacgcgg gctgcagctg cgcgtgcacg ccccgcagcc ggcgatcatg
      421 cagggccacc gccagctgct cgcgcagctg ctgaccaatt tgctcgagaa tgccatcaag
      481 tacgtgccgg ccgggggcac gatcgacctg gacgtggcat tgaacgcggc gcgcatcgaa
      541 ttgaccgtca gcgacgacgg gcccggcatc ccggaccttg cgcacggctc gatgctcttg
      601 ccgttccggc ggctcgagcg cgaccacggc gcgcccggga gcggcctcgg gttgagcctc
      661 gtcaatgcag tcgtgcgact gcaccaggga cagctcgagc tgcgcagcat gtcgcccggg
      721 ctgtgcgtca gctgcacgtt cgctgctgcc tcgggcgccg ccgctgtgac cggtgccgcc
      781 gctcccgccg gcgtcgcggc cgctgcggcg ggcgcaactg cagcggcgcc ggctgccgtc
      841 gccagggcat cgccgttcgc gaacgcgccc agtgcgccca tcgtggaccg tacggagagc
      901 accggcgggt tcgtgatcac gggagtggtg aagccgaagg gctgagtttt ggcgatgtcc
      961 agggcgaaga cattcacggc gttgctggtc gcatccgcgt tttgcgcggc cgctacgccg
     1021 ccggtctacg aagtgcgcga cgacgacggg cgcctgctct cgctccgcgc gccgccacaa
     1081 cgcatcgtca gcctcgctcc cggcgccacc gccatgctat tcgccgccgg cggaggcacg
     1141 cgcgtcgtcg gcacgccgga attctcggtc gagccggagg cggcgcgcgc catcccgcgc
     1201 atcggcgaca gccatggttt cgacctcgag cgaatcctgg cgctccatcc ggacctgctg
     1261 gtcgcctgga gcggaggcgc ttcggccgcg cagctgttgc cgttcgagcg cgcggcgctt
     1321 ccggtctacc accatcgcgt gacgcgactc gacgagttgc cgcggagcat cgagcgcctc
     1381 ggggtcctgc tgcacaccga gcccgaggcc cggcgcgcag ccgccgcgct cgctgcgcgc
     1441 atcaacgcga tccacgccca ctatcggcgc gacaatccgc cgcggttgtt gctgcaggtc
     1501 tgggatcgcc cggtctacac gcttggcggc ggccaactca ccgccgacgc cgccgaggcc
     1561 tgcggctacc gcaacgtcta cgccgagttg cacgacgctg cgccggctgt gtcactcgag
     1621 tcgatcgtgg cgcgcgatcc ggacgtgatc ctggtgctcg cggcggacga gggcacggcg
     1681 cagcagtggc tcgatcactg gaaatcgctg ccggccctgc gcgcggtgcg caaccaccgc
     1741 gtcgtcagct tcgtcgatgc gcgattgagc cgcctcgggc ccgagactgt cgacgctact
     1801 gaagcgctgt gcgagaagct cgccggggtc gcgccgcctg cgtccacgcc ataggccagg
     1861 cgcacggtac ggcgccgacg gccgcgctcg gcgcgccaca acgtgcggcg cgggcggacc
     1921 ccgatgatgg ccagagacgg aatggcggcg gcgcgaagct cagcggcgtg caatcacgcg
     1981 ggctggacta ttgcgcagcg gtgaacccgc actgctgctg ctccatcgcg atctgcttcg
     2041 t
//
LOCUS       DASRDG010000098         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_250392, whole genome shotgun sequence.
ACCESSION   DASRDG010000098 DASRDG010000000
VERSION     DASRDG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250392"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..4880
                     /locus_tag="VFA35_01625"
     CDS             9..4880
                     /locus_tag="VFA35_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HZT54898.1"
                     /translation="MFPLRVATGDAPPIAKFAAAPFGVIELGDPLVPVTLRHVQGDLR
                     PAAGGGQVRVKALQTDAEILAWYNRLKQYDASQIGAKAAGLPEREWFTVENDTDAGVD
                     KRGRLVQRKTERFVATRELSLLNRDAGAKRLDLPQLNGGDPRPFEVVGIPIGAPGYHV
                     VEIESLRLGQSLLDKRAPMFVRTGVLVTNLGVHFKLGRENSVVWVTTLDRGKPVEGAE
                     IAVNDCNGKRLWSGRTDANGLAKVAQALDADAVSCTAESGLFVTARKPDAKGVVDTAF
                     VFSSWQKGIESWRFNLPTGRGAEPDVRAATVFDRTLFRAGETVSMKHFVRFETSAGLA
                     ALKPEDLPTRLKIVHQGSGQAFVQPLQWNGTRSAQSSWAIPPAAKLGLYTVALERESA
                     NAQRRREWSSGNFRVEAFRVPLVDARVSGPKAVAVAPKALPIAVQLNYFSGGPMAQQP
                     ARASALLKSRSVRFDGYDEFSFEPPREPNPAPGNSGNDEDTEAPPEARDARLVADKLA
                     LTTDRNGAANFRLENLPPLTRPSELTAELSYNDPNGEVQTASTTIPLWPSATVLGIKT
                     GAWASRRGKVKFTALALDTSGRPIKGRHIEVRGRLHQTLSTRKRMVGGFYAYDNRTEL
                     KDFGSLCSGSSDEQGLLQCEAELNDAGQVELIASAKDAGGNSVQAAASVWVTKQGELW
                     FAQDNDDRIDVLPEKKRYEPGETATLQVRMPFREATALVAVEREGVIDMRVVTLRGDD
                     PSIALKIEPGWGPNVYVSVLSVRGRIRDVPWYSFFTWGWKEPMNWVRSFWYEGREYQA
                     PTALVDLSKPAFKFGVAALQVGIRAHELQVGVTTDKPQYTVRQKAIAKVKVAFGGKPL
                     AGAEIAFAAVDEGLLALRDNDSWDLLHAMIQPRAWGVETSTAQGEIVGRRHYGRKAVA
                     AGGGGGRGATRELFDTLLLWKANLVLDANGEATVEVPLNDSLTSFRLVAIADYSNAAG
                     TAQFGTGSASIRVTQDLQMLAGLPPLVREGDEFGALLTLRNTTAREMKVRATLQGTAN
                     AGAGAEIVRTPIALPAQDVVVAAGAAKELVWPVRIPADAFSVTWEAGADEQGGPATPE
                     RSKDRIKVTQLVAPAVPVRVLQATLQQLEGTLTLPIAAPADALPEAGVKRGGIQIAAQ
                     PKLSGALPGMRRYFETYPFSCLEQKVSKSVGLKDPKLWAAVANALPTYLDSDGLASYF
                     PPRVEDGPRGSDRLTAYVLAATSEAGFALPDAARDAMLAGLTAFVEGRIERRFWSPKA
                     DLDVRKLAAIEALSRHGRAQPKMLGSINLVPNTWPTAAVIDWLRILKQVPGIPNAAKR
                     LDEANQILRSRLSYSGTTLTFANEQDDFWWWLMDSADANAARLILAVIDDPAWREDIP
                     RMVVGALARQRAGAWLTTTANLWGALALDRFSAKFESVKITGRTAATLADQGGAIDWA
                     AKPGGGAITLAWPAAPGPLNVAQDGAGKPWLTVQSLAAVPLRAPLRAGYSVARSVSAV
                     EQKDKAGWSRGDVVRVRLEVDAQTDMSWVVVSDPVPGGATILGSGLGRDSSIATRTER
                     SAGHAWPAFEERGVEAFRSYFEYLPKGRHVIEYSVRLNNPGRFSLPPTRVEAMYAPQT
                     FGELPNAIVEVAP"
BASE COUNT          778 a         1705 c         1816 g          636 t
ORIGIN      
        1 atgcctcgat gttcccgctt cgggtcgcga ccggcgacgc gccgccgatc gccaagttcg
       61 cggccgcacc gttcggcgtg atcgagctcg gcgacccgct cgtgccggtg acgctgcgcc
      121 atgtgcaggg cgacctgcgg cccgcggcgg gtggcgggca ggtgcgcgtg aaggcgctgc
      181 agaccgatgc cgagatcctc gcctggtaca acaggctgaa gcagtacgac gcgagccaga
      241 tcggcgcgaa ggcggccggc ctgcccgagc gcgagtggtt cacggtcgag aacgacaccg
      301 acgccggggt tgacaagcga ggaaggctcg tccagcgcaa gaccgagcgc tttgtggcga
      361 cgcgcgagct ctcgctgctg aaccgcgatg ccggcgcgaa gcggctcgat ctgccgcagc
      421 tgaacggcgg cgacccgcgg ccgttcgagg tcgtcggcat cccgatcggc gcgcccggct
      481 atcacgtggt cgagatcgaa tcgctgcgcc tgggccagtc gctgctcgac aagcgcgcgc
      541 cgatgttcgt gcgcaccggc gtgctcgtca ccaacctcgg cgtgcacttc aagctcggcc
      601 gcgagaacag cgtcgtctgg gtcaccacgc tcgaccgcgg caagccggtc gagggcgccg
      661 agatcgcggt caacgactgc aacggcaagc ggctctggtc gggccgcacc gatgcgaacg
      721 gcctggccaa ggtcgcgcag gcgctcgatg ccgatgcggt gagctgcacg gccgagtccg
      781 gcctgttcgt gacggcccgc aagcccgacg cgaagggcgt cgtcgacacc gcgttcgtgt
      841 tcagtagctg gcaaaaaggc atcgagtcgt ggcgcttcaa cctgccgacc gggcgcggcg
      901 ccgagcccga cgtgcgcgcc gcgacggtgt tcgaccgcac gctgtttcgc gccggcgaga
      961 ccgtctcgat gaagcacttc gtgcgcttcg agacctccgc cggcctggcc gcgctgaagc
     1021 ccgaggatct gccgacgcgc ctgaagatcg tccaccaggg cagcggccag gcgttcgtgc
     1081 agccgctcca atggaacggc acgcgcagcg cgcagtcgag ctgggcgatc ccgccggcgg
     1141 ccaagctcgg gctctacacc gtcgcgctcg aacgcgagag cgcgaacgcg cagcggcgcc
     1201 gcgagtggag cagcggcaac ttccgcgtcg aggcgttccg cgtgccgctg gtcgatgcgc
     1261 gcgtcagcgg gcccaaggct gtggcggtcg cgccgaaggc gttgccgatc gcggtgcagc
     1321 tgaactactt ctccggcggc ccgatggcac agcagccggc gcgcgcgtcg gcgctgctga
     1381 agagccgatc ggtgcgcttc gacggctacg acgagttctc gttcgagccg ccgcgcgagc
     1441 cgaaccccgc gcccggcaac agtggcaacg acgaagacac cgaggcgccg cccgaagcgc
     1501 gcgacgccag gctcgtcgcc gacaagctgg cactgacgac cgaccggaac ggcgccgcga
     1561 acttcaggct cgagaacctg ccgccgctga caaggccgag cgagctcacc gccgagctca
     1621 gctacaacga cccgaacggc gaggtgcaga ccgcgtcgac gacgattccg ctgtggccga
     1681 gcgcgaccgt gctcggcatc aagaccggcg cgtgggcgag ccgccgcggc aaggtcaaat
     1741 tcaccgcgct cgcgctcgat acatcggggc ggccgatcaa agggcggcac atcgaggtgc
     1801 gcggccggct gcaccagacg ctcagcacgc gcaagcgcat ggtcggcggc ttctacgcct
     1861 acgacaaccg caccgagctg aaagacttcg gatcgctgtg cagcggcagc agcgacgagc
     1921 aggggctgct gcagtgcgag gccgagctga acgacgcggg ccaggtcgag ctgatcgcga
     1981 gcgcgaagga tgcgggcggc aattcggtgc aggcggcggc cagcgtgtgg gtcacgaagc
     2041 agggcgaact ctggttcgcg caggacaacg acgaccgcat cgacgtgctg cccgagaaga
     2101 agcgctacga acccggcgag accgcgacgc tgcaggtgcg catgccgttt cgcgaagcga
     2161 ccgcgctggt cgcggtcgag cgcgagggcg tcatcgacat gcgcgtcgtc acgctgcgcg
     2221 gcgacgaccc gagcatcgcg ctgaagatcg agccgggctg gggcccgaat gtctacgtca
     2281 gcgtgctgag cgttcgcggc cgcatccgcg acgtgccctg gtactcgttc ttcacctggg
     2341 gctggaagga gccgatgaac tgggtgcgct cgttctggta cgaggggcgc gagtatcagg
     2401 cgccgaccgc gctggtcgat ctgagcaagc ccgcgttcaa gttcggcgtc gccgcgctgc
     2461 aggtcggcat ccgcgcgcac gagttgcagg tcggcgtgac gaccgacaag ccgcagtaca
     2521 cggtgcggca gaaggcgatc gcgaaggtca aggtcgcgtt cggcggcaag ccgctggccg
     2581 gcgccgagat cgcgttcgcg gcggtcgacg aaggcctgct cgcgctgcgc gacaacgact
     2641 cgtgggacct gctgcacgcg atgatccagc cgcgcgcctg gggcgtcgag accagcactg
     2701 cgcagggcga gatcgtcggc cgccgccact acggccgcaa ggcggtcgcg gccggcggcg
     2761 gcggcgggcg cggcgcgacg cgcgagctgt tcgacacgct gctgctatgg aaggcgaacc
     2821 tggtgctcga cgcgaacggc gaggcgacgg tcgaggtgcc gctgaacgac tcgctgacga
     2881 gcttcaggct ggtcgcgatt gcggactatt cgaatgcggc aggcaccgca cagttcggca
     2941 ccggcagcgc gagcatccgc gtcacgcaag acctgcagat gctcgccggc ctgccgccgc
     3001 tggtgcgcga gggcgacgag ttcggcgcgc tgctgacgct gcgcaacacg acggcgcgcg
     3061 agatgaaggt gcgcgcgacg ctgcaaggca cggccaacgc gggcgcgggt gccgagatcg
     3121 tgcgcacgcc gatcgcgctg ccggcgcagg acgtggtcgt cgcggccggc gcggccaagg
     3181 agctggtgtg gccggtgcgc attccggccg acgcgttcag cgtcacttgg gaggcgggcg
     3241 cggacgagca gggcggtccg gcgacacccg agcgatccaa ggaccgcatc aaggtcacgc
     3301 aactcgtcgc gcccgcggtg ccggtgcgcg tgctgcaggc gacgctgcag cagctcgaag
     3361 ggacgctgac gctgccgatc gccgcgcccg ccgatgcgct gcccgaggcc ggcgtgaagc
     3421 gcggcggcat ccagatcgcg gcgcagccca aactcagcgg cgcactgccc ggcatgcgcc
     3481 gctacttcga gacctacccg ttcagctgcc tcgaacagaa ggtctcgaag tcggtcggcc
     3541 tgaaagaccc gaagctgtgg gcggcggtcg cgaacgcgct gccgacctac ctcgacagcg
     3601 acggcctggc gagctacttt ccgccgcgcg tcgaagacgg cccgcgcggc agcgaccggc
     3661 tcaccgccta cgtgctcgcc gcgacgagcg aggccggctt cgcgctgccc gacgcggcgc
     3721 gcgacgcgat gctcgcgggc ctgacggcgt tcgtcgaggg ccgcatcgag cgcaggttct
     3781 ggtcgccgaa ggccgacctc gacgtgcgca agctcgccgc gatcgaagcg ctctcgcgcc
     3841 acggccgcgc gcagccgaag atgctgggct cgatcaacct cgtgcccaac acctggccga
     3901 ccgcggcggt gatcgactgg ctgcgcatcc tgaagcaggt gccgggcatt ccgaacgccg
     3961 cgaagcggct ggacgaagcg aaccagatcc tgcgcagccg gctgagctac tcgggcacga
     4021 cgctgacgtt cgccaacgag caggacgact tctggtggtg gctgatggac agcgccgacg
     4081 cgaacgcggc gcggctgatc ctcgccgtga tcgacgaccc ggcgtggcgg gaagacatcc
     4141 cgcgcatggt cgtcggcgcg ctcgcgcgcc agcgcgccgg cgcgtggctg acgacgaccg
     4201 cgaacctgtg gggcgcgctc gcgctcgacc ggttctcggc gaagttcgag tcggtgaaga
     4261 tcaccggccg caccgcggcg acgctggccg accagggcgg cgcgatcgac tgggccgcca
     4321 agcccggcgg cggcgcgatc acgctcgcgt ggccggccgc gcccgggccg ctgaacgtcg
     4381 cgcaggacgg cgccggcaag ccgtggctca cggtgcaaag cctcgccgcc gtgcccttgc
     4441 gcgcgccgct gcgcgcgggc tacagcgtcg cgcgcagcgt cagcgccgtc gagcagaaag
     4501 acaaggcggg gtggtcgcgc ggcgacgtgg ttcgcgtgcg cctcgaggtc gacgcgcaga
     4561 ccgacatgag ctgggtcgtc gtcagcgacc cggtgcccgg cggcgcgacc atcctcggct
     4621 cgggcctggg ccgcgactcc tcgatcgcga cccgcaccga gcgcagcgcc ggccatgcct
     4681 ggccggcgtt cgaagagcgc ggcgtcgagg cctttcgcag ctacttcgag tacctgccga
     4741 agggcaggca cgtgatcgag tacagcgtgc gcctgaacaa ccccggccgg ttctcgctgc
     4801 cgccgacgcg ggtcgaggcg atgtacgcgc cgcagacctt cggcgagctg ccgaacgcga
     4861 tcgtcgaggt cgcgccgtga tgcgttggcg cattgcaagc gccgccgcgc tgctgtgcgt
     4921 ggcgtcgctc gcgca
//
LOCUS       DASRDG010000099         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1625495, whole genome shotgun sequence.
ACCESSION   DASRDG010000099 DASRDG010000000
VERSION     DASRDG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625495"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFA35_01630"
     CDS             <1..741
                     /locus_tag="VFA35_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HZT54899.1"
                     /translation="EIEVFDEPDVAARKHRAGLRRLIALQIKDALKYLEKNIPDLQKM
                     SVAYLLVGDGSADELRDQIIDVALDRAFLADALPTDAAAFARTIEGGRGRLTLIANEV
                     ARSAGTVLLEHTAAVRKLKDARAPKEVTEDITAQLGRLVPKRFVADTPWAQLAHLPRY
                     LKAIVMRLDKQRADPARDAQRLAELRPIESRYWRHLAERKGVADARLAEYRWLLEELR
                     VSLFAQELRTPQPVSVKRLEKVWTQLSS"
     gene            790..1044
                     /locus_tag="VFA35_01635"
     CDS             790..1044
                     /locus_tag="VFA35_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZT54900.1"
                     /translation="MSYVWMAVIGFVVGLIARAILPGTQSLGIILTAVLGIVGSFIAG
                     FAGRALGWYTEGQPAGFIASIVGAIVLLFIVGKLKGSSSS"
     gene            1049..1573
                     /locus_tag="VFA35_01640"
     CDS             1049..1573
                     /locus_tag="VFA35_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54901.1"
                     /translation="MTMSTALPADWVVVPDTPREIEAVVKHCRRLVTKRAMVAAGVSM
                     VPIPGVDWVTDVAVLMKLIPEINRAFGLTPEQVERLAPDRRLVVYKTISAGGGLLVGR
                     LVTRELIIKLLKLVGVRLTTQQAAKYVPIAGQAVSAALTFSSLKFVCEQHIQQCASVS
                     KQLMLQAPPAEPAH"
     gene            1592..1668
                     /locus_tag="VFA35_01645"
     tRNA            1592..1668
                     /locus_tag="VFA35_01645"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1626..1628,aa:Pro,seq:tgg)
     gene            1718..1794
                     /locus_tag="VFA35_01650"
     tRNA            1718..1794
                     /locus_tag="VFA35_01650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1752..1754,aa:Arg,seq:tct)
     gene            complement(1804..>2512)
                     /locus_tag="VFA35_01655"
     CDS             complement(1804..>2512)
                     /locus_tag="VFA35_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZT54902.1"
                     /translation="LVLARARELIADAQQIRRDVQQLEGLAIGSLAIGLGPFAAGTLG
                     RAALALMTQRHPQLMLRMEVADANTLCERLHRRQLDLFVADTRDLKKQPGLKIARLPN
                     LPVSFFVRPKHPLRRRKQVTLAQAMAYPVAGPQLPTAVAAHFDQQIQRSERAVLNVTC
                     DDAGTLLHLALNADAVILAAHAPALDTGVNALVPLPIAGLAGMQTRYSLVTLAGRTLS
                     AAATAYTGVLAELLGPR"
BASE COUNT          382 a          855 c          878 g          397 t
ORIGIN      
        1 gagatcgagg tcttcgacga gcccgatgtc gcggcgcgca agcaccgcgc cggcctgcgc
       61 cggctgatcg cgctgcagat caaggatgcg ctgaaatacc tcgagaagaa catccccgat
      121 ctgcagaaga tgagcgtggc ctacctgctc gtcggcgacg gcagcgccga cgagctgcgc
      181 gaccagatca tcgatgtcgc gctcgaccgc gccttcctcg ccgacgcgct gcccaccgat
      241 gccgccgcgt tcgcgcgcac gatcgaaggc ggtcgcgggc gcctgacgct gatcgcgaac
      301 gaggtcgcgc gcagcgccgg cacggtcctg ctcgaacaca ccgcggccgt gcgcaagctg
      361 aaggacgcgc gtgcgccgaa ggaagtcacc gaggacatca ccgcgcagct cggccgcctc
      421 gtgccgaagc gcttcgtcgc cgacacgcct tgggcgcagc tcgcgcacct gccgcggtat
      481 ctgaaggcga tcgtcatgcg cctcgacaag cagcgcgccg acccggcccg cgacgcgcag
      541 cgcctcgcgg aactgcgccc gatcgagtcg cgctactggc gccatctggc cgaacgcaag
      601 ggcgtggccg atgcgcgcct ggccgagtac cgctggctgc tcgaggagct gcgcgtcagc
      661 ctgttcgcgc aggagttgcg tacgccgcag ccggtcagcg tgaagcggct cgagaaggtc
      721 tggacgcagc tctcgtcgtg aagcgtcatg cgctacattg cagcgatctt tcaccacaag
      781 gaacgaacaa tgagctacgt ctggatggca gtcatcggtt tcgtcgtcgg gctgatcgcg
      841 cgggcgatcc tgccgggcac gcagagtctc gggatcatcc tgacggccgt actcggcatc
      901 gtcggatcgt tcatcgccgg atttgccggg cgcgcgctgg gttggtacac cgagggacag
      961 ccggccggct tcatcgcgtc gatcgtcggt gcgatcgtgc tgctgttcat cgtcggcaag
     1021 ctcaaggggt cgtcgtcgag ttgatcgcat gacgatgagc accgcgctgc cggccgactg
     1081 ggtcgtcgtg cccgacacgc cgagggagat cgaggcggtc gtcaagcact gccgtcgcct
     1141 cgtcacgaag cgtgcgatgg tcgcggccgg cgtctcgatg gtgccgatcc cgggggtcga
     1201 ctgggtcacc gacgtcgcgg tgctgatgaa gctgatcccc gagatcaacc gtgccttcgg
     1261 cctcacgccc gagcaggtcg aacgcctcgc ccccgaccgg cggctggtgg tctacaagac
     1321 gatctcggct ggcggcggcc tgctggtcgg ccggctcgtc acgcgcgagc tgatcatcaa
     1381 gctgctcaag ctcgtcggcg tgcggctgac gacgcagcag gccgcgaaat acgtgccgat
     1441 cgcggggcag gcggtctcgg cggcgctcac gttctcgtcg ctgaagttcg tctgcgagca
     1501 acatatccaa cagtgcgcgt cggtgtcgaa acaactgatg ctgcaggcac cgccggcgga
     1561 gcccgcgcac tgagtacaat gcgaggttcg tcggggcgta gcgcagcctg gtagcgcacc
     1621 tggtttggga ccaggtggtc gtgagttcga atcccaccgc cccgaccatc cctcacggca
     1681 ccgccctgcg gccgcccggc cccgtggtct tccaacgcag cccgtagctc aactggatag
     1741 agcaccgacc ttctaagtcg gatgtcgggg gttcgagtcc ctccgggctg gccaacccca
     1801 ttttcatcgc ggccccagca actcggccag cacgccggtg taggcggtgg cggcggccga
     1861 gagcgtgcgc ccggcgagcg tcaccaggct gtagcgcgtt tgcatgcctg cgaggccggc
     1921 gatcggcagt ggcaccagcg cgttgacccc ggtgtcgagc gccggcgcat gggctgccag
     1981 gatcaccgcg tcggcgttca gcgccaggtg cagcagcgtg cctgcgtcgt cgcaggtcac
     2041 gttgaggacg gcccgttcgc tgcgctggat ctgctggtcg aagtgcgcgg ccaccgcggt
     2101 cggcagttgc gggccggcca ccggatacgc catggcctgc gccagcgtca cctgcttgcg
     2161 ccggcgcagc ggatgcttcg gccgcacgaa gaacgagacc ggcaggttgg gcaggcgcgc
     2221 gatcttcagg ccgggctgct tcttcaggtc gcgcgtgtcg gccacgaaca ggtcgagctg
     2281 ccgccggtgc agccgctcgc acagcgtgtt ggcgtccgcc acttccatgc gcagcatgag
     2341 ctgcgggtgg cgctgcgtca tcagcgccag cgccgcacgg cccagcgtgc cggcggcgaa
     2401 ggggccgagc ccgatcgcga ggctgccgat ggcgaggccc tcgagctgct gcacgtcgcg
     2461 ccggatctgc tgcgcatcgg cgatcagttc gcgggcgcgc gcgagcacca gt
//
LOCUS       DASRDG010000100         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1280462, whole genome shotgun sequence.
ACCESSION   DASRDG010000100 DASRDG010000000
VERSION     DASRDG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280462"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..710)
                     /locus_tag="VFA35_01660"
     CDS             complement(51..710)
                     /locus_tag="VFA35_01660"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZT54903.1"
                     /translation="MPLGFDPQELPIVGIDDHLPALTAQVLRSAAVRQRFLMPPVWTP
                     EIKAEPRFGERGPTHASVLVALVEREELTVLLTQRTDHLSDHPGQVSFAGGRVEPTDA
                     DAAATALREAHEEIGLEASFVDVLGAMPTYTTGTGFIVTPIVALVRPGFTVAADPFEV
                     ADVFEVPLDFILDRANHQRHFRMLGTARRDYWAIPWLHRYIWGATAAMLLILERTLRA
                     D"
     gene            complement(793..1146)
                     /gene="rplS"
                     /locus_tag="VFA35_01665"
     CDS             complement(793..1146)
                     /gene="rplS"
                     /locus_tag="VFA35_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZT54904.1"
                     /translation="MDLIQTLEHEEIARLNKTIPPFAPGDTVIVSVNVVEGTRKRVQA
                     FEGVVIAKRNRSLNSSFIVRKISSGEGVERTFQLYSPLIASIEVKRRGDVRRAKLYYL
                     RSRSGKSARIKEKLA"
     gene            complement(1247..2005)
                     /gene="trmD"
                     /locus_tag="VFA35_01670"
     CDS             complement(1247..2005)
                     /gene="trmD"
                     /locus_tag="VFA35_01670"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZT54905.1"
                     /translation="MRFDIITLFPDLFAPHLTRGITRRAFESGQVDVHLWPLRDFADN
                     AYRRVDDRPYGGGPGMVMLVEPLERALAAVREARAEAVPAPVVHFAPTGRRIDQAVVR
                     EFAASAGAVLLCGRYEGVDQRFIERHVDIELSLGDFVLSGGELPALALLDAVARLQDG
                     VLGDARSHQQDSFEDGLLDCPHYSRPEVLGSGEGAQPVPAVLLSGHHADIARWRREQS
                     LALTARRRPDLIAAARAAGRLAQADEKYLAGLVP"
BASE COUNT          338 a          689 c          758 g          316 t
ORIGIN      
        1 cggtttcggg tatcaggtgt cgggcatcag gtatcacggc gtgatgcgca tcagtcggcg
       61 cgcagcgtgc gctccaggat gagcagcatc gcggccgtcg cgccccagat gtaacggtgc
      121 agccatggaa tggcccagta atcgcggcgc gccgtcccga gcatccggaa atgccgctgg
      181 tgattcgcgc gatcgaggat gaagtcgagc ggcacctcga acacgtcggc cacctcgaac
      241 ggatcggcgg ccaccgtgaa cccgggccgc accagcgcga cgatcggcgt gacgatgaag
      301 ccggtgccgg tggtgtaggt cggcatcgcg ccgagcacgt cgacgaagct cgcctccagg
      361 ccgatctctt cgtgcgcctc gcgcagcgcg gtcgccgccg catcggcatc ggtcggctcg
      421 acccgcccgc cggcgaagct gacctggccc gggtggtcgc tcaggtggtc ggtgcgctgg
      481 gtcagcagca ccgtcagctc ctcgcgctcg accagcgcga ccaacaccga cgcatgcgtg
      541 ggaccgcgtt cgccgaagcg cggctcggcc ttgatctcgg gcgtccagac cggcggcatc
      601 aggaagcgct ggcgcaccgc ggccgagcgc aatacctgcg cggtcagtgc cggcaggtgg
      661 tcatcgatgc cgacgatggg cagctcttgc ggatcgaaac cgaggggcat ggcggatgga
      721 atcgggaatg caaaaggccg cccacaggag gcggccttcg gtccgaggca gcggactacc
      781 tcgcggtcgc tatcaggcga gcttctcttt gatgcgcgcc gacttgcccg agcggctgcg
      841 caggtagtac agcttggcgc ggcgcacatc gccgcggcgc ttgacttcga tcgaggcgat
      901 cagcgggctg tagagctgga acgtgcgttc cacgccttcg ccgctggaga tcttgcgcac
      961 gatgaagctg ctgttcaggc tgcggttgcg cttggcgatg acgacgcctt cgaacgcctg
     1021 gacgcgcttg cgggtgcctt cgacgacgtt gacgctgacg atcacggtgt cacccggcgc
     1081 gaacggcggg atggtcttgt tgagccgagc gatttcttcg tgctcgaggg tctggatcag
     1141 gtccatggtt tctccaagtg atcgtgccgg cgcgtgttga tggagttggg tgaagcgcca
     1201 agacgagttg gcgcttctgc gcggcagagg atcgaaaagc ctttgattat ggcacaagcc
     1261 cggcgaggta cttctcatcg gcctgcgcca ggcgcccggc cgcgcgcgca gcggcgatca
     1321 gatcggggcg gcggcgcgcg gtcagcgcga gcgactgctc gcgccgccag cgggcgatgt
     1381 cggcgtggtg gccggaaagc agaacggccg ggacgggctg cgcgccctca cccgagccca
     1441 gcacttcggg ccggctgtaa tgcgggcagt cgagcaggcc gtcctcgaag ctgtcctgct
     1501 gatgcgaccg ggcgtcgccg agcacgccgt cctgcaggcg cgcgaccgcg tcgagcagcg
     1561 cgagcgcagg cagttcgccg cccgagagga cgaagtcgcc gaggctgagt tcgatgtcga
     1621 catggcgctc gatgaaacgc tggtcgacgc cctcgtagcg cccgcacagc agcaccgcgc
     1681 cggcggaggc ggcgaactcg cgcaccaccg cctggtcgat gcgccgcccg gtcggcgcga
     1741 agtggacgac gggcgccggc accgcctcgg cgcgtgcctc gcgcaccgcc gcgagcgcgc
     1801 gttcgagcgg ctcgaccagc atcaccatgc ccggcccgcc gccgtagggg cggtcgtcga
     1861 cacggcggta ggcgttgtcg gcaaagtcgc gcaagggcca gaggtgcacg tcgacctgcc
     1921 ccgactcgaa cgcgcgccgc gtgatgcccc gcgtcagatg cggcgcgaac aggtcgggga
     1981 acagcgtgat gatgtcgaag cgcatggcgt gccaggccgg ggcggatcag taatccaagc
     2041 cccaatcgac cgtgatcagc ttctgctcga ggctgaccgc atcgacatac gcgccaacga
     2101 a
//
LOCUS       DASRDG010000101         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1398091, whole genome shotgun sequence.
ACCESSION   DASRDG010000101 DASRDG010000000
VERSION     DASRDG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398091"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1017
                     /locus_tag="VFA35_01675"
     CDS             106..1017
                     /locus_tag="VFA35_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT54906.1"
                     /translation="MKPAFILRLAAAGLLAFSALQAGAQSAVAPTQLTGTLAKVRETG
                     SITIGHRESSIPFSYLSARGEPIGYSVDLCKLLVDAISEEVGREVAIKWKPVTSESRI
                     PAVVSGQVDLECGSTTSNLERQKLVSFSPTMFVSGTKLMVKKGSPIKSFRDLAGKRVV
                     VTAGTTNEKTMRELAVKFKIDMILSVAADHAASFAQLQAGQVDAFATDDVLLYGLLAQ
                     NKAQADYAVVGDFLSYDPYGIMYRKGDAQLARLVNDTFHALAEDREIERRYNRWFVRK
                     LPSGVSLDLPMSAQLETIIQTMVSRTE"
     gene            1086..2141
                     /locus_tag="VFA35_01680"
                     /pseudo
     CDS             1086..2141
                     /locus_tag="VFA35_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572417.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
     gene            2163..>3301
                     /locus_tag="VFA35_01685"
     CDS             2163..>3301
                     /locus_tag="VFA35_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate carboxypeptidase"
                     /protein_id="HZT54907.1"
                     /translation="MKKTLLLSLAALACAASVTAFAQAAKADGALLDAAQKRKDAFVK
                     DLATLANIDSGTDDAKGLAQVEAVLAQRLKDLGASVEVVAAPPAAGKAVIGKLRGTGT
                     QNIMLMIHYDTVFGVGEAAKRPFKITGNKASGPGVADAKGGLLMIVYALDIARERGFK
                     DYNTLTVLFNPDEEKSSLGSRDLITKLSADQDYVLVFEPPEADVVTVATNGIAYVHLD
                     VKGRASHAGSAPEAGRNAAVELSNQIIQLKDLGSPAKGTTVNWTVIRAGDRVNIIPEV
                     ASATADMRMSDVQEIQRVQADADTIIQNKLIADTDVKVRVENRRPPFSKNADSDRLAG
                     VADGIYKELGKSITPVSMRYGTDAGFAYNPKTGKPVVLDGMGIVG"
BASE COUNT          608 a         1142 c         1085 g          466 t
ORIGIN      
        1 gaagtgggaa ggcgagctgc tgtcggaaga ggcggccgcg gccaacctga agcagatcga
       61 cgccgaggcg gccgcgctgg cgcacccgat cccggcctga ccgcgatgaa gcccgccttc
      121 atcctccgcc tcgcggccgc cgggctgctc gctttcagcg cgctgcaggc cggcgcgcag
      181 agcgcggtgg cgccgacgca gctgaccggc acgcttgcga aagtgcgcga gactggctcg
      241 atcacgatcg gccaccgcga gtcgtcgatc ccgttctcgt acctgtcggc gcgcggcgag
      301 ccgatcggct attcggtcga cctgtgcaag ctgctggtcg atgcgatcag cgaggaggtc
      361 ggccgcgagg tcgcgatcaa gtggaagccg gtgacctcgg agtcgcgcat tccggcagtc
      421 gtcagcggcc aagtcgacct ggagtgtggc tcgaccacca gcaacctcga acgccagaag
      481 ctggtgagtt tttcgcccac gatgttcgtg tcgggcacca agctgatggt gaaaaagggc
      541 tcgccgatca agtcgttccg cgacctggcc ggcaaacgcg tcgtcgtgac cgccggcacg
      601 acgaacgaga agacgatgcg cgagctggcc gtcaagttca agatcgacat gatcctgagc
      661 gtggcggcgg accacgcggc ctcgttcgcg cagctccagg ccggccaggt cgatgcattc
      721 gcgaccgacg acgtgctgct ctacggcctg ctcgcgcaga acaaggcgca ggccgactac
      781 gccgtcgtcg gcgacttcct ctcgtacgac ccttacggga tcatgtaccg caagggcgat
      841 gcccagctcg cgcggctggt caacgacacc ttccacgcgc tcgccgaaga ccgcgagatc
      901 gaacgccgct acaaccgctg gttcgtgcgc aagctgccct cgggcgtgag cctggacctg
      961 ccgatgagcg cgcagctcga gaccatcatc cagacgatgg tgtcgcgcac cgaatgagtc
     1021 gcgccgccgc acgcttttcc ctttcacctg tcaaaggaac gcccatgcgc ctcgttcaac
     1081 gtctgatgcg cggcctcgcc gccgccgtcc tcgccaccag cctcggcgcg ttcgcgcaaa
     1141 ccgcgccggc gcccaaggcc aacgtcgtcg tgctcgccac cggcggcacg atcgccggcg
     1201 ccggcgcgag cgccgcgaac agcgccacct accaggccgc caaggtgccg gtcgacaagc
     1261 tgatcgccgg cgtgcccgag ctcgccgaca tcgccaacgt gcgcggcgag caggtgttcc
     1321 agatcgcctc cgagagcttc acgaacgaga agctggtgac gctggccaag cgcgtcgccg
     1381 cgctcgccaa agacccgggc gtcgacggca tcgtgatcac ccacggcacc gacacgctgg
     1441 aagagacctc gttcttcctg accctgacgg tgcacaccga caagccgatc gtcgtcgtcg
     1501 gctcgatgcg cccgggcacc gcgatgtcgg ccgacggcac gctgaacctg tacgacgcgg
     1561 tcgccgtcgc ggccgcgaag gacgcgcgca gcaagggcgt gctgatcgtg atgaacgacg
     1621 acatcctgtc gggccgcgac tcgggcaagc gcatcaacat caagaccagc gcgttcggta
     1681 gccagtgggg cgcgctcggt tcgatcgtcg agggcaagac ctactggttc cgtgcgccgg
     1741 tcaagcgcca cacgatggcc agcgagttcg acatcgaatc gatcacgacg ctgccgatgg
     1801 tcgccatcgt ctacggttcg ggcgagatgg gcacctgggc ggccgaagcc gccggcaagg
     1861 ccggcgcgaa ggcgatcatc cacgccggca ccggcaacgg ctaggtgccc gactacgagg
     1921 tcgagacgct gaagaagctg cgcgcgagcg gcgtgcagat catccgctcg gcgcgcgtgc
     1981 ccgacggctt cgtgctgcgc aactccgagc agcccgacga caagtacgac tgggtcgtcg
     2041 cgcacgacct gaacccgcag aaggcgaaga tcctcgcggc cgtggcgctg accaagacca
     2101 gcgacccgaa ggaactgcag cgcatcttct ggtcttactg agcgcagcca cgcgagaagc
     2161 gaatgaagaa gacgctgctg ctgtcactgg ctgcgctggc ctgcgccgcc tccgtcaccg
     2221 cgttcgctca ggccgccaag gccgacggtg cgttgctgga tgccgcgcag aagcgcaagg
     2281 acgccttcgt caaggacctg gccacgctgg cgaacatcga ctcgggcacc gacgacgcca
     2341 agggcctggc ccaggtcgaa gccgtgttgg cgcagcgcct gaaggacctc ggcgccagcg
     2401 tcgaggtcgt cgcggcgccg ccggccgcgg gcaaggcggt gatcggcaag ctgcgcggta
     2461 cgggcacgca gaacatcatg ctgatgatcc actacgacac cgtcttcggc gtcggcgagg
     2521 ccgccaagcg cccgttcaag atcaccggca acaaggcctc cggccccggc gtggccgatg
     2581 ccaagggcgg tctgctgatg atcgtctacg cgctggacat cgcgcgcgaa cgcggcttca
     2641 aggactacaa cacgctgacc gtgctgttca accccgacga ggagaaaagc tcgctcggct
     2701 cgcgcgacct gatcacgaag ctgtcggccg accaggacta cgtgctggtg ttcgagcccc
     2761 ccgaggccga tgtggtgacc gtcgcgacga acggcatcgc ctatgtccat ctcgacgtca
     2821 agggccgcgc atcgcatgcc ggctcggcgc cggaagccgg ccgcaacgcg gcggtcgagt
     2881 tgtcgaacca gatcatccag ttgaaagacc tgggcagccc ggccaaaggc acgaccgtca
     2941 actggaccgt gatccgcgcc ggcgaccgcg tcaacatcat ccctgaagtc gccagcgcca
     3001 ccgccgacat gcgcatgtcc gacgtgcagg agatccagcg cgtccaggcc gacgccgaca
     3061 cgatcatcca gaataagctg attgccgaca ccgacgtgaa ggtgagggtc gagaaccgcc
     3121 gcccgccgtt cagcaagaac gccgacagcg atcgcctggc cggcgtggcg gacggcatct
     3181 acaaggaact cggcaagtcg atcacgccgg tgtcgatgcg ctacggcacc gatgccggtt
     3241 tcgcctacaa ccccaagacc ggcaagccgg tcgtgctcga cggcatgggc atcgtcggcg
     3301 a
//
LOCUS       DASRDG010000102         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1049741, whole genome shotgun sequence.
ACCESSION   DASRDG010000102 DASRDG010000000
VERSION     DASRDG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049741"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="VFA35_01690"
     CDS             complement(<1..1853)
                     /locus_tag="VFA35_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZT54908.1"
                     /translation="MSLDANAAAYRADGRPVARDAFYALACDPQRSVVVEACAGAGKT
                     WMLVSRILRALLAGAQPHEILAITFTRKAAGEMRERLNEWLREFSAPGASEASRVEAL
                     VQRGVPAHEARELAPQLASLHERVLRAGRPVEVRTFHAWFSQLLRAAPLELLGELGLQ
                     PEMDLIEDLDDHRTEVFRRFHAAVLADAALRDDYAALIARRGRSQLRKWLEAALHKRV
                     EFELADAAGVLDDSVPPPALERPGEHPAQVLLGAAWHASLRELALALGLGGVRAQDAA
                     TGLVFALEQADAQARFERVWAALFTEKNTPRKQLGKVDALARIQDELDAIAMQVRQLD
                     AHTEHLRMARLARVLLAEFAAYKRARGLADMADLERCGLALLRDATLAGWVQERLDAR
                     IRHVLIDEFQDTSPLQWHALHAWLSAYAGAGGGASGQRPPGLFIVGDPKQSIYRFRRA
                     EPRVFAAAQRFVVEALGGTVLACDHTRRNAPELLAALNTVFDQAQRANEFSGFRDHTT
                     ELASDPQAGVFVLPRVPRPPRAARGAAADTPAPWRDSLTTPRHAPEELLREQEAARVA
                     LAIHALVTRGGVVPAEIFVLSRKRQSLRLVAQALQQLQVPYAAAEDAALMDA"
     gene            complement(1850..>2161)
                     /locus_tag="VFA35_01695"
     CDS             complement(1850..>2161)
                     /locus_tag="VFA35_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZT54909.1"
                     /translation="AGLREKVKEPLEDTQLAFYAALMRSRTELPLSARYLALDGSKGL
                     EEIAHPAVEASAAALVDGLADDLRRLRAGAGLPALGVGATCAYCAARGICRRDQWTDA
                     T"
BASE COUNT          272 a          794 c          810 g          285 t
ORIGIN      
        1 gcgtccatca gtgcggcgtc ctcggctgcc gcatagggca cctgcagctg ctgcagggcc
       61 tgcgcgacca ggcgcagcga ctggcgcttg cgactgagca cgaagatctc ggccggcact
      121 acgccgccgc gcgtcacgag cgcatggatc gccagcgcga cgcgggcggc ctcctgctcg
      181 cgcagcagct cctcgggcgc atggcgcggc gtcgtcaggc tgtcgcgcca gggcgccggc
      241 gtgtcggcgg cggcgccgcg cgcggcgcgc ggcgggcgcg gcacacgcgg cagcacgaac
      301 acgccggcct gcgggtcgct tgccagctcg gtcgtgtgat ctcgaaatcc gctgaactcg
      361 ttggcgcgct gcgcctggtc gaacacggtg ttgagcgccg cgagcagctc gggcgcgttg
      421 cgccgggtgt ggtcgcaggc cagcaccgtg ccgcccagcg cctcgacgac gaagcgctgg
      481 gccgccgcga acacgcgcgg ctcggcgcgc cgaaagcggt agatgctctg cttcgggtcg
      541 ccgacgatga acagccccgg cggccgctgc ccgctcgcgc ccccgcctgc gccggcgtag
      601 gccgagagcc aggcgtgcag cgcgtgccac tgcagcgggc tggtgtcctg gaactcgtcg
      661 atcagcacgt gacggatgcg cgcgtcgagc cgctcctgga cccagccggc gagcgtcgcg
      721 tcgcgcagca gcgcgaggcc acagcgctcc aggtcggcca tgtcggcgag gccgcgggcg
      781 cgcttgtagg ccgcgaattc ggcgagcagc acgcgcgcga gccgcgccat gcgcaggtgc
      841 tcggtgtgcg cgtcgagctg gcggacctgc atcgcgatcg cgtcgagctc gtcctggatg
      901 cgcgcgagcg catcgacctt gccgagctgc ttgcgcggcg tgttcttctc ggtgaacagc
      961 gccgcccaga cgcgctcgaa gcgcgcttgc gcgtcggcct gttcgagcgc gaacacgagc
     1021 ccggtggccg cgtcctgcgc cctgaccccg cccaggccca gcgcgagcgc gagttcgcgc
     1081 agcgacgcgt gccaggcggc accgagcagc acctgcgccg ggtgctcgcc ggggcgttcg
     1141 agcgcgggcg gcggcacgct gtcgtcgagc acgccggccg cgtcggcgag ctcgaactcg
     1201 acacgcttgt gcaacgccgc ctccagccac ttgcgcagct ggctgcggcc gcggcgcgcg
     1261 atcagcgcgg cgtagtcgtc gcgcagcgcc gcgtcggcga gcacggcggc gtggaagcgg
     1321 cggaagacct cggtgcggtg atcatcgagg tcttcgatca ggtccatctc gggctgcagc
     1381 ccgagctcgc ccagcagctc cagcggcgcg gcgcgcagca gttgcgagaa ccacgcgtgg
     1441 aaggtgcgca cctcgaccgg ccggcccgcg cgcagcacac gctcgtgcag gctcgcgagc
     1501 tgcggcgcga gttcgcgcgc ttcgtgcgcg ggcacgccgc gctgcaccag cgcctcgacg
     1561 cgcgacgcct ccgacgcgcc tggcgcgctg aattcgcgca gccactcgtt caggcgctcg
     1621 cgcatctcgc cggccgcctt gcgcgtgaac gtgatcgcga ggatctcgtg cggctgcgcg
     1681 ccggccagca gcgcgcgcag gatgcgcgag accagcatcc aggtcttgcc tgcgccggcg
     1741 caggcttcga cgacgacact gcgttgcggg tcgcaggcga gcgcgtagaa cgcatcgcgc
     1801 gccactggcc ggccgtcggc gcgataggcc gccgcattgg cgtcgagact catgttgcgt
     1861 cggtccattg gtcgcggcgg cagatgccgc gcgccgcgca gtacgcgcag gtcgcgccca
     1921 cgccgagtgc gggcaggccg gcaccggcgc gcagccgacg caggtcgtcg gccaggccgt
     1981 cgaccagcgc cgcggcgctc gcttcgacgg ccgggtgtgc gatctcctcc aggcccttgc
     2041 tgccgtcgag cgcgaggtag cgcgcgctca gcggcagctc ggtgcgcgag cgcatcaagg
     2101 cagcgtagaa cgcgagctgg gtgtcttcga gcggctcctt caccttctcg cgcaggcccg
     2161 c
//
LOCUS       DASRDG010000103         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1524387, whole genome shotgun sequence.
ACCESSION   DASRDG010000103 DASRDG010000000
VERSION     DASRDG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524387"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFA35_01700"
     CDS             <1..508
                     /locus_tag="VFA35_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZT54910.1"
                     /translation="SAIPGIGSPQMTVGLIKGGINTNVVPDRVTFRLDRRMIPEENPA
                     EVEATIREVIAEAAASRPGITVEIKRLLLANAMQPLAGNKPLVDALRKHGEAVFGEPI
                     PTSGTPLYTDVRLYGEQGIPAAIYGAGPRTVFESNAKRADEHLVLEDLRRATKVVARS
                     LFDLLTAA"
     gene            772..1233
                     /locus_tag="VFA35_01705"
     CDS             772..1233
                     /locus_tag="VFA35_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZT54911.1"
                     /translation="MQRHLRTANAIAKRALEAGHHPFGAVLVGPDHETVLSTQGNIDT
                     VNHAEAVLTRMAAKAWTPDELWGCTLYTTVEPCCMCAGTQYWAHIGRLVYGMDEHRLL
                     QFTGDHPENATLALPCRAVFAAGQKDIRVWGPVPEVEAEIAALHVAFWKRA"
     gene            1253..2095
                     /locus_tag="VFA35_01710"
     CDS             1253..2095
                     /locus_tag="VFA35_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HZT54912.1"
                     /translation="MAWAGSLTLGYRRDVLRFEPRTVLHDRHDGPLRVLASLYPETPA
                     ICHNVLVHPPGGLVGGDALDIDITLDAGAHALLTTPGATRFYRSAGAPATQRLRATLA
                     DGARLEWLPLETIAYSGCLGENLMRFTLAPGAEMIGWDLLALGLPASEQAFVRGRFTQ
                     CVEMPGRWLERGVIDALDARLLDSPLGWAGHRALGTLWFAAGSALPNARRESLLAAAR
                     DAVQDDPLQRSSGATAVHDGVIVLRVLAPRVEPAMALLTRVWAAWRREAWQLAATPPR
                     VWRT"
     gene            2231..2557
                     /locus_tag="VFA35_01715"
     CDS             2231..2557
                     /locus_tag="VFA35_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54913.1"
                     /translation="MNTATSTRILATRTLAALALLLCATAQAQSVRKCTIDGHVVFQS
                     SPCPLEARPAVAAPQAAADPASAPKKKALAELLRERDGTDRVRPPAREFSGDGATVLR
                     ARMGAV"
     gene            complement(2752..2919)
                     /locus_tag="VFA35_01720"
     CDS             complement(2752..2919)
                     /locus_tag="VFA35_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HZT54914.1"
                     /translation="MTKTTTRLWDPAEHLQSDEDMAAYLEAAPEEGDASLFAAAVGDI
                     ARAKGMSQLAR"
     gene            complement(3104..>3575)
                     /locus_tag="VFA35_01725"
     CDS             complement(3104..>3575)
                     /locus_tag="VFA35_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZT54915.1"
                     /translation="YPQRCRRLVLAATAAGGLMVPGKLSVLSKMASPRRYTDADYMAE
                     IGGELYGGALRHRPELLREHAHHMRAPRGRGYYYQQLAMLGWTSVFGLPLLRQPTLVI
                     AGSDDPIVPLVNAKILAALIPKAKLHVVDDGHLFLVSRTDEIAELVRGFLAAKA"
BASE COUNT          534 a         1310 c         1224 g          507 t
ORIGIN      
        1 gtcggccatc cccggcatcg gcagcccgca gatgaccgtc gggctcatca agggcggcat
       61 caacaccaac gtcgtgcccg accgggtgac cttccggctc gaccgccgga tgatcccgga
      121 agagaacccg gccgaggtcg aggcgacgat ccgtgaggtg atcgccgagg cggcggcgag
      181 ccgtccgggc atcacggtcg agatcaagcg cctgctgctc gccaacgcga tgcagccgct
      241 ggccggcaac aagccgctgg tcgacgcgct gcgcaagcac ggcgaggcgg tgttcggcga
      301 gccgatcccg acctcgggca cgccgctcta caccgacgtg cggctgtacg gcgagcaggg
      361 catcccggcg gcgatttacg gcgcaggccc gcgcacggtg ttcgagtcga acgccaagcg
      421 cgccgacgag cacctggtgc tcgaagacct gcgccgcgcg accaaggtgg tcgcgcgttc
      481 actgttcgac ctgctgacgg ccgcctgaac gcccgagagg cgtcccggcg acctcttgca
      541 cccagcgctg cgctcctcac cgccacagaa atcgcccctg ctggatgccg cgaagtccag
      601 cagcggagcg actgtacgaa ttgttcgcgg tgatcgagaa acgcaccacg ggctgggcgc
      661 accgcggcgc gcgaagctga ggcagactcg cgcagtggca agcaccagga tgaccgcctc
      721 ttgaacccga cccgacccgc gccgcccggc accgacccga caccggcgca gatgcagcgc
      781 cacctgcgca ccgccaacgc gatcgcgaag cgtgcgctcg aggccggcca ccacccgttc
      841 ggcgcggtgc tcgtcgggcc ggaccacgaa accgtgctga gcacgcaggg caacatcgac
      901 accgtgaacc acgccgaggc cgtgctcacg cgcatggccg cgaaggcctg gacgcccgac
      961 gagctgtggg gctgcacgct ctacaccacg gtcgagccct gttgcatgtg cgccggaacg
     1021 cagtactggg cgcacatcgg ccggctcgtc tacggcatgg acgaacaccg cctgctgcaa
     1081 ttcaccggcg atcacccgga gaacgcgacg ctggccctgc cgtgccgcgc ggtcttcgcg
     1141 gccggccaga aggacatccg cgtctggggg ccggtgcccg aggtcgaggc cgagatcgcc
     1201 gcgttgcacg tcgccttctg gaagcgcgcg tgaagacggc gatgcggcgc tgatggcctg
     1261 ggccggctcg ctgacgctgg gctaccggcg cgacgtgctg cgctttgaac cgcgcaccgt
     1321 gctgcacgat cgccacgacg gtccgctgcg cgtgctcgcg agcctgtacc ccgagacgcc
     1381 ggcgatctgc cacaacgtgc tcgtgcaccc gccgggcggc ctcgtcggcg gcgacgcgct
     1441 cgacatcgac atcacgctcg acgccggcgc ccacgcgctg ctcacgaccc ccggtgcgac
     1501 gcgcttttac cgcagcgccg gcgctcccgc gacccagcgc ctgcgcgcga cgctggccga
     1561 cggcgcgcgc ctcgaatggc tgccgctcga gacgatcgcc tacagcggct gcctcggcga
     1621 gaacctgatg cgcttcacgc tcgcgcccgg cgccgagatg atcggctggg acctgctcgc
     1681 gctcggcctg ccggcctcgg agcaggcctt cgtgcgtggc cgcttcacgc aatgcgtcga
     1741 gatgcccggc cgctggctgg agcgcggcgt gatcgatgcg ctcgacgcgc gcctgctcga
     1801 ctcaccgctc ggctgggccg gccaccgcgc gctcggcacg ctgtggttcg ccgccggcag
     1861 cgcactgccg aacgcgcgcc gcgagtccct gctcgcggcc gcgcgcgatg cggtgcagga
     1921 cgacccgctg cagcgcagca gcggcgcgac cgcggtgcac gacggcgtga tcgtgctgcg
     1981 cgtgctcgcg ccgcgggtcg agccggcgat ggcgctgctc acgcgcgtct gggccgcgtg
     2041 gcggcgcgag gcctggcagc tagccgcgac gccgccgcgt gtctggcgca cctgaacgcg
     2101 atcgcgcggc gacgccgcgg tgacgcattc gacatctgct tacaaccccg cggcgcgtcg
     2161 gcaaccgtgt cgccgtgatt cgtgactttc ttcgcaggcg cggcgcacgc gcgtccctag
     2221 actgccacgc atgaacactg ccacctccac ccgcatcctc gcgacccgca cgctggcggc
     2281 gctggccctg ctgctgtgcg ccacggcgca ggcgcagtcg gtgcgcaagt gcacgatcga
     2341 cggccatgtc gtgttccagt cgtcgccctg cccgctcgaa gcgcgcccgg ccgtggccgc
     2401 gccgcaggcc gccgccgacc ccgcctccgc gccgaagaag aaggccctgg ccgagctgct
     2461 gcgcgaacgc gacggcaccg accgcgtgcg cccgcccgcg cgcgagttct cgggcgacgg
     2521 cgcgaccgtg ctgcgcgcgc gcatgggcgc ggtctgagcc tgcggcgcgc ccgcggtgat
     2581 gcgcaggctg gcgtccgagg cgccgtgcca cagcgcgccc aagtgacggc acagcgagca
     2641 cgcgcattcc atgacgtggt cgagcctcgc gtcgagctcg aacgcgacgc gacggcaatg
     2701 gcaggaagct cgatacgtga gccgtgcgtt catggtgggg cctgagaagg ttcagcgcgc
     2761 gagttgcgac atgcccttgg cgcgagcgat gtcacccact gccgccgcga aaagagaggc
     2821 atcgccttct tccggtgccg cctcgaggta ggctgccatg tcttcatcgg attgaagatg
     2881 ttcagcggga tcccagagac gagtggttgt cttggtcatc tctttgctcc acgtacctgc
     2941 gcgccagttc cttggccttc ttgatgtcgc gcgcctgggt ggatcttttg ctaccgacga
     3001 gcaatggcaa ccagtgatca ccgcggcgaa cgaagtagac cctgtcgcgc atcccgcgcc
     3061 tcaaaccatc gtattcgcac ctggtgaccg tttcgcttgc gtgtcacgcc ttcgcggcga
     3121 gaaacccgcg gaccagctcc gcgatctcgt cggtgcggct gaccaagaag agatggccat
     3181 cgtcaacgac gtgcagcttg gccttgggga tcagcgcggc cagtattttg gcgttgacca
     3241 gcggcacgat cgggtcgtcg ctgcccgcga tcaccagggt cggctgccgc agcagcggca
     3301 ggccgaacac gctggtccag cccaacatcg ccagctgctg gtagtagtag ccgcgcccgc
     3361 gcggcgcgcg catgtggtgg gcgtgctcgc gcagcaactc gggacgatgg cgcaaggccc
     3421 cgccatacag ctcgccgccg atctcggcca tgtagtcggc gtccgtatag cggcgcggac
     3481 tcgccatctt gctcagtacc gacagcttgc ccggcaccat cagcccgccc gccgcggtgg
     3541 cggcgagcac cagccggcgg cagcgctgcg ggtac
//
LOCUS       DASRDG010000104         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1049891, whole genome shotgun sequence.
ACCESSION   DASRDG010000104 DASRDG010000000
VERSION     DASRDG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049891"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFA35_01730"
     CDS             <1..415
                     /locus_tag="VFA35_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54916.1"
                     /translation="AVNARDRIGTGPWRNAKGVVVANNVTELHGTNNLSKQTALTERG
                     DIVNGRGDTPNMHDILTGAQPDGTFIAGNVNSTCGNWTQSGEGSAMVGHHNKAGLDES
                     VPAKSWNSSHLTKGCSADALKGTGGAGLFYCFAAD"
     gene            complement(461..1495)
                     /locus_tag="VFA35_01735"
     CDS             complement(461..1495)
                     /locus_tag="VFA35_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HZT54917.1"
                     /translation="MARRIRKTLWRPPPRRSLGALTRATLRRGTRAVLTASAKALKAA
                     AKPPRRPPKPRRPAAAPGEWLTGAAAGPAGTRRYRLFKPAGARARAPLPLLVMLHGCD
                     QDAASFARSTRLQTLATREGFMLLFPEQERIANVHGCWNWFETRSGRAFTEAASLIAA
                     IDQVCTLHGADPSRVAIAGLSAGASMAAFVALHYPQRFKAVAMHSGVAPGAAHSTASA
                     LAAMQGRRRPHALAPTPGLPPLLVIQGMADHLVRPGNGPAAARLWAGAAGALPAAPRT
                     VQRGARRPMTLTDFKQRGRIVATLCEVAGLGHAWSGGAANQPYADPTGPDASRLIWAF
                     AARAFSATAL"
     gene            complement(1505..1858)
                     /locus_tag="VFA35_01740"
     CDS             complement(1505..1858)
                     /locus_tag="VFA35_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HZT54918.1"
                     /translation="MIANLLVGLVVAIHLYIVVLEMFLWDTPYGRKVFGNSAEFATAS
                     KVLAANQGLYNGFLAAGLIWGLSLGAAGQPVQLFFLGCVIVAGVYGAVSVGLRILFVQ
                     ALPAALAVASILLGI"
BASE COUNT          329 a          768 c          769 g          254 t
ORIGIN      
        1 cgccgtcaac gcgcgcgacc gcatcggcac cggcccgtgg cgcaacgcca agggcgtcgt
       61 cgtcgccaac aacgtgaccg agctgcacgg cacgaacaac ctcagcaagc agaccgcgct
      121 gaccgagcgc ggcgacatcg tcaacggccg tggcgacacg ccgaacatgc acgacatcct
      181 gaccggtgcg cagcccgacg gcaccttcat tgccggcaat gtcaacagca cctgcggcaa
      241 ctggacgcag agtggcgaag gctcggcgat ggtcggtcac cacaacaagg ccgggctcga
      301 cgagagcgtg ccggccaagt cgtggaactc gtcgcacctg acgaaaggct gcagcgccga
      361 tgcgctgaag ggcaccggcg gcgccggcct gttctactgc ttcgcggccg actgatgacg
      421 gccccaaggt cgcctgcggc gccccggcgc tggcctgcga tcacagcgct gtcgcgctga
      481 acgcgcgcgc cgcgaacgcc cagatcaggc gtgacgcgtc cggcccggtc gggtcggcgt
      541 agggctggtt cgcggcgccg ccgctccagg cgtggccgag cccggccact tcgcacagcg
      601 tcgcgacgat gcgcccgcgc tgcttgaaat ccgtcaacgt catcgggcgc cgcgcgccgc
      661 gctgcacggt gcgcggcgcc gccggcaacg cgcccgccgc gcccgcccac agacgtgccg
      721 cggccggccc gttgcccggc cgcacgaggt ggtcggccat gccctggatc accagcagcg
      781 gcggcaggcc cggcgtcggt gccagcgcgt gcggccggcg ccggccctgc atcgcggcga
      841 gcgcgctcgc ggtcgagtgc gcggcgccgg gggcgacgcc ggagtgcatc gcgacggcct
      901 tgaaccgctg cgggtagtgc agcgccacga aggccgccat gctcgcgccg gccgacagcc
      961 cggcgatcgc gacccgcgac gggtcggccc cgtgcagcgt gcagacctgg tcgatcgccg
     1021 cgatcaggct cgcggcctcg gtgaaggcgc ggcccgagcg cgtctcgaac cagttccagc
     1081 acccatgcac gttcgcgatg cgctcctgct ccgggaacag cagcatgaag ccctcgcgcg
     1141 tcgcgagcgt ctgcaggcgc gtgctgcgcg cgaagctggc ggcgtcctgg tcgcaaccgt
     1201 gcagcatcac cagcagcggc agtggcgccc tggcccgcgc gcccgctggc ttgaagagcc
     1261 ggtagcggcg cgtgccggcc gggccggccg cggcgccggt gagccactcg ccgggcgccg
     1321 cggcgggacg gcgcggcttg ggcggccggc gtggcggttt cgccgcggcc ttcaaggcct
     1381 tggccgatgc ggtcagcacc gcgcgcgtgc cgcggcgcaa ggtcgcgcgg gtcagcgcgc
     1441 cgaggctgcg ccgcggcggc gggcgccaga gggtcttgcg aatgcggcgt gccatggggt
     1501 tcggtcagat ccccagcagg atcgatgcca ccgccagcgc cgccggcagc gcctgcacga
     1561 acaggatccg caagcccacg ctgaccgccc cgtacacgcc ggcgacgatc acgcagccga
     1621 ggaagaagag ttgcaccggc tgcccggccg cgcccaggct cagcccccag atcagcccgg
     1681 cggcgagaaa gccgttgtac aagccctggt tcgccgccag caccttcgag gcggtggcga
     1741 actcggcgct gttgccgaag accttgcggc cgtagggcgt gtcccacagg aacatctcga
     1801 gcacgacgat gtagaggtgg atcgccacca ccaggccgac gagcaggttc gcgatcattc
     1861 gggcatcctc cagggggttt ggaaacgaca gggcgttcga tgcgcgccat tctcgccgcg
     1921 ggagccggcg tcgtgccggc ggcggccggt cggggttcgt gaacgcgttc gttgcgcgaa
     1981 agcgctatgc cagcgccgta ggctgcgagc tgcaagtcaa actagactgg tgcggcagaa
     2041 gacaacgtga cgcccatgcc atcagccgaa aggactccgc ggagaacgac catggccatc
     2101 gaactgccga aggaggcgca
//
LOCUS       DASRDG010000105         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_942725, whole genome shotgun sequence.
ACCESSION   DASRDG010000105 DASRDG010000000
VERSION     DASRDG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942725"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFA35_01745"
     CDS             <1..1071
                     /locus_tag="VFA35_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HZT54919.1"
                     /translation="VGIAGTSAQQLGRMGLRTMGYFVAVTLLALLVGLFAVNLVKPGV
                     GVNLPADVDQRAQAIAANAEKLTPQQHLLGAVPESAIRAAAENQVLQLVVFSLLFAIA
                     VARIGEKGRPVVELCRSIADAMFRFTGFVMALAPLAVAAAIAATIGQSGFDVLRNLAA
                     LVATLYGALLAFFVLVLLPIALWTRLPFRRFLRLVREPALIAFTTTASEAALPKALEN
                     MERLGVPRRVVGFVLPLGYSFNLDGSALYLSLAAMFVAQAAGRAISVGDQVAMVATLL
                     LSSKGIAAVPRASLVVLAGTLAQFHLPLTGIALILGVDVLMDMARTATNVIGNCLASC
                     VIARWEGVLGEPAGDRRGGDAG"
     gene            1171..1542
                     /locus_tag="VFA35_01750"
     CDS             1171..1542
                     /locus_tag="VFA35_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54920.1"
                     /translation="MSKTTLVVLSDPRGGEEALGRLFNALAAAYDFAQRHQEVQIVFQ
                     GAGTRWTGVVTQPDHPAHALYRAVEDKVVGASSACAVVFGARDAAESNGFALLADNAV
                     PGTTGLPSLAAFAREGTVITF"
     gene            1546..2502
                     /locus_tag="VFA35_01755"
     CDS             1546..2502
                     /locus_tag="VFA35_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZT54921.1"
                     /translation="MTRDPFHAGELEVQQRAGQRALAASNGAMVEDHLTPGACRFAAQ
                     QRLVAVAARDASGWPRAALWLGAPGFLRCADERTFAVALPRLCAQDPVRAGLAAGASL
                     GLLLVDLRTRRRLRVNGSVARVDDVELTLAVREAFANCPKYVQRREPDARAPAPGGSA
                     ATVAGTVLDAERTARIARADTVFVASGHKARGLDASHRGGEAGFVRVLDPRTLRFPDY
                     PGNGMFQTLGNLAVDPRAGVAVVDFDHGRVLSLTGTVRTRFDEAEDPAWPTGGTGRYW
                     ELDVRDWLEHPLPSAPGWRLVEPSPFNPRSWSQSGGAREGRV"
     gene            2512..>3039
                     /locus_tag="VFA35_01760"
     CDS             2512..>3039
                     /locus_tag="VFA35_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZT54922.1"
                     /translation="MPAALASREVVLERLLATFRDQGYDGASLAELSAATGLGKSSLY
                     HHFPGGKVDMAAQVLAHLDAGLEQTLFASLRGPGSPQKRLDSMLEGLSRFYERGRRAC
                     LLERLCASVDRQRFRRPLAHVFEKWIDAVAALARDAGLPEALARERAEDLVIRVEGAL
                     VVCAGTGDTRCFTRTL"
BASE COUNT          357 a         1070 c         1150 g          462 t
ORIGIN      
        1 gtcggcatcg cgggcacgtc ggcgcagcag ctcggccgca tggggctgcg cacgatgggc
       61 tacttcgtcg cggtgacgct gctggcgctg ctcgtggggt tgttcgcagt caacctcgtc
      121 aagcccggcg tcggcgtgaa cctgccggca gacgtcgacc agcgcgcgca ggcgatcgcc
      181 gcgaacgcgg agaagctcac gccgcagcag cacctgctcg gcgccgtgcc cgaaagcgcg
      241 atccgcgccg cggccgagaa ccaggtgctg cagctcgtgg tcttcagcct gctgttcgcg
      301 atcgcggtgg cgcgcatcgg cgagaagggc cggccggtgg tggaactgtg ccggtcgatc
      361 gccgacgcga tgttccggtt caccggcttc gtcatggcgc tggcgccgct cgccgtcgcc
      421 gcggcgatcg cggccacgat cggccagagc ggcttcgacg tgctgcgtaa cctcgccgcg
      481 ctggtcgcga cgctgtacgg cgcgctgctg gcgttcttcg tgctggtgct gctgccgatc
      541 gcgctgtgga cgcggttgcc gttccggcgc ttcctgcggc tggtgcgcga gccggcgttg
      601 atcgcgttca ccaccacggc gagcgaggcg gcgctgccga aggcgctcga gaacatggag
      661 cggctcggcg tgccgcggcg ggtggtcggg ttcgtgctgc cgctcggcta ctcgttcaac
      721 ctcgacggca gcgcgctcta cctgtcgctg gcggcgatgt tcgtggcgca ggcagccggc
      781 cgcgcgatct cggtcggcga ccaggtggcg atggtcgcga ccctgctgct gtcgagcaag
      841 ggcatcgccg cggtgccgcg cgcgtcgctg gtggtgctcg ccggcacgct ggcgcagttc
      901 cacctgccgc tgaccggcat cgcgctgatc ctcggcgtcg acgtgctgat ggacatggcg
      961 cgcacggcga ccaacgtgat cggcaactgt ctcgcgagct gcgtgatcgc gcgctgggaa
     1021 ggagtgctcg gggagcccgc cggggaccgg cgcggcggcg acgcggggtg agagggcgag
     1081 aggcttgcgt ccggcggcgg cccgttctat tgtaccgatc gttcggttca acatcggaac
     1141 cgcattcccc tcgaccaagg agttggctgc atgtccaaga ccacgctcgt cgtcctgtcc
     1201 gatccccgcg gcggtgaaga ggccctcggc cgcctgttca acgccctcgc cgccgcctac
     1261 gacttcgccc agcgccacca ggaggtgcag atcgtgttcc agggcgccgg gacgcgctgg
     1321 accggcgtcg tcacgcagcc cgatcacccg gcgcacgcgc tgtatcgcgc ggtcgaggac
     1381 aaggtggtcg gcgcgtcttc ggcgtgcgcc gtcgtgttcg gcgcccgcga cgcggccgag
     1441 agcaacggct tcgcgctgct cgcggacaac gccgtgcccg gcaccaccgg gttgccgagc
     1501 ctcgctgcgt tcgcgcgcga gggcacggtc atcaccttct gagccatgac gcgcgatccg
     1561 ttccacgcgg gcgagctcga ggtgcagcag cgcgcaggac agcgcgcgct ggcggcgagc
     1621 aacggcgcca tggtggagga ccacctgacg cccggcgcct gccgtttcgc ggcgcagcag
     1681 cgcctggtcg ccgtggcggc gcgcgacgcg tccggctggc cgcgtgcggc gctgtggctc
     1741 ggcgcccccg gcttcctgcg ctgcgcggac gagcgcacgt tcgcggtcgc gctgccgcgg
     1801 ctttgcgcgc aggacccggt gcgcgcgggg ctggccgcgg gtgcgtcgct cggcctgttg
     1861 ctcgtcgatc tgcgcacgcg gcggcggctg cgcgtgaacg gcagcgtggc ccgcgtcgac
     1921 gacgtggagc tcacgctggc ggtgcgcgag gcgttcgcca actgcccgaa gtacgtccaa
     1981 cgccgcgagc ccgacgcgcg cgcgccggcg ccgggcgggt ccgccgcgac ggtcgcgggc
     2041 acggtgctcg acgccgagcg gacggcgcgg atcgcgcgcg cggacaccgt gttcgtggcc
     2101 agtgggcaca aagcgcgcgg actcgacgcc tcgcaccgcg gcggggaggc tgggttcgtc
     2161 cgcgtgctcg atccgcgcac gctgcgcttc ccggactatc cgggcaacgg catgttccag
     2221 acgctgggca atctcgcggt cgatccgcgc gccggcgtcg cagtcgtcga tttcgaccac
     2281 gggcgggtgc tgtcgctcac gggcacggtc aggacgcggt tcgacgaggc ggaggatccc
     2341 gcgtggccga cgggcggcac cggccgctac tgggagctcg acgtgcgcga ctggctcgag
     2401 cacccgctcc cgtcggcgcc gggctggcgg ctggtcgagc cctcgccgtt caatcccagg
     2461 tcctggtccc agtctggtgg cgcgcgcgag ggacgagtat aggacctgcc gatgcccgcg
     2521 gcgctcgcca gcagagaagt ggtgctcgag cggttgctcg cgacgttccg cgaccagggc
     2581 tacgacggcg cgtcgctcgc ggagttgtcg gccgccaccg ggctcggcaa gtcgagcctc
     2641 tatcaccatt ttccgggcgg caaggtggac atggcggcgc aggtgctcgc gcacctcgac
     2701 gccgggctcg agcagacgct gttcgcatcg ctgcgcggtc cgggctcgcc gcagaagcgg
     2761 ctggacagca tgctcgaagg cctgtcgcgc ttctacgagc gcggcaggcg cgcgtgtctg
     2821 ctcgagcggc tgtgcgcgag cgtcgatcgc cagcgcttcc gccgcccgct cgcgcacgtc
     2881 ttcgagaagt ggatcgacgc ggtcgctgcg ctcgcccgcg acgccggtct gcccgaggcg
     2941 ctcgcgcgcg agcgcgccga ggacctggtg atccgcgtgg agggggcgct cgtcgtgtgc
     3001 gccggcacgg gcgacacccg ctgcttcacc cgcacgctc
//
LOCUS       DASRDG010000106         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_17304, whole genome shotgun sequence.
ACCESSION   DASRDG010000106 DASRDG010000000
VERSION     DASRDG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17304"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFA35_01765"
     CDS             complement(<1..773)
                     /locus_tag="VFA35_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HZT54923.1"
                     /translation="MEADPTVSVASLHVYPIKSCGGIALTEALLIETGIEFDRAWMVV
                     DAAGVFVTQRELPRMALIQPVLKTSEMVLRAPGMLALHVALDRVEAPARATVWDDEVA
                     AYDMGDLCAQWFSDFLGRPLRLVRFDPEHKRLSDHRWTGDLDAENAFADGFPVLVSSV
                     AALAELNRRLRAQGLADVTMARFRPNLVLDGLDAHGEDALDEIAFATLDGPVRLRFVK
                     PCARCTIPDVDPLTAATGHAVGDTLAAYRADARLGGKVT"
     gene            882..1997
                     /locus_tag="VFA35_01770"
     CDS             882..1997
                     /locus_tag="VFA35_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZT54924.1"
                     /translation="MNNFDVQVLGAGIVGRCLALALARAGLSVALRPEAARAPAGDDV
                     RAYALNAASVELLRGLKVWDALPAHAATPVLDMHVQGDAHAAAIDFSAWQQRVGALAW
                     ITDAAVLERELAAAVRFAPHITVVDAGAQVNAALTALCEGKASATRAALGIAFERVDY
                     GHRAIAARLTSTRGHQGMARQWFRAPDVLALLPFDTPQPQHSFALVWSLPDARANELL
                     ALDAAAFEAELMQATKGEAGTLTLASERAAWPLMQANASAWCGPGWVLLGDAAHVVHP
                     LAGQGLNLGLADVAALVKVIREREVWRPLGDAKLLRRYARERVVPTWAMGQVTDGLLR
                     LFAEDAPAVRELRNRGLTLVNKLSPLKRWLTARALNS"
     gene            2007..2738
                     /locus_tag="VFA35_01775"
     CDS             2007..2738
                     /locus_tag="VFA35_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HZT54925.1"
                     /translation="MNTLLRPILALTLGLAFAHAALADEAAIRKNLTERLPELPKIDE
                     VSKTAIPGLYEVRIGTELLYTDERGDHVIQGSIVETATRTDLTQARIDKITAIDFAAL
                     PLKDAIVWKQGTGARKLVVFADPNCGYCKKFETEMQQVKNVTVYTFLVPILGGDSPDK
                     SRNIWCAKDNATVWRDWMIKGAPAPRSMGNCDVSALQRNIAMAKKYRVNGTPALVFED
                     GKRVPGALPPDEVEKRLLAGRGAKG"
     gene            2948..>3987
                     /locus_tag="VFA35_01780"
     CDS             2948..>3987
                     /locus_tag="VFA35_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HZT54926.1"
                     /translation="MLTFAITAFDPRAHLFRVSVRIPAPAAEQRLSLPAWIPGSYLVR
                     EFARHLSGLSASQGRRAVPLAQLDKATWLARCEGGATLTVSYLVYAFDTSVRAAFLDA
                     QRGFFNGTSVFLRVEGRAAEPHRLELRGLPAGWQVATALTPVEVDAFGRGVYAAAGYD
                     ELVDHPVELGRFWRGRFEAHGVPHEYVVAGALPDFDGARLLADAQRICEAQIRFWHPL
                     GKPPFERYVFLHNLVDEGHGGLEHRASTALISPRRDMPAVGKPDSSDGYVNVLGLVSH
                     EYFHTWNVKRLRPAEFASVDYTQENYTELLWFFEGFTSYYDDLFLLRTGLIDTARYLR
                     LVARTVSGVLSMP"
BASE COUNT          600 a         1401 c         1411 g          575 t
ORIGIN      
        1 aaggtgacct tgccgccgag ccgcgcatcg gcgcggtagg cggcgagcgt gtcgccgacg
       61 gcgtggccgg tggccgcggt gagcgggtcg acatcgggga tagtgcagcg cgcgcagggc
      121 ttcacgaacc gcaggcgcac cgggccgtcg agggtcgcga acgcgatctc gtcgagcgca
      181 tcttcgccgt gggcgtcgag gccgtcgagc acgaggttgg gccgaaagcg cgccatcgtc
      241 acgtcggcca ggccttgcgc gcgcaggcgg cggttcagtt cggccagcgc cgcgaccgag
      301 ctgacgagga ccggaaagcc gtcggcgaaa gcgttctcgg cgtcgagatc accggtccag
      361 cggtgatccg acaagcgctt gtgctcgggg tcgaagcgca ccagccgcaa aggccggccg
      421 agaaaatcgc tgaaccactg cgcgcacagg tcgcccatgt cgtaggccgc gacctcgtcg
      481 tcccagacgg tcgcgcgcgc cggcgcctcg acgcggtcga gcgcgacgtg cagcgccagc
      541 atcccgggcg cgcgcagcac catctcgctg gtcttgagca ccggctggat cagcgccatg
      601 cgtggcagtt cgcgctgcgt gacgaagacg ccggccgcat cgacgaccat ccaggcgcgg
      661 tcgaactcga tgccggtctc gatcagcaag gcctcggtca gcgcgatgcc gccgcacgac
      721 ttgatcgggt agacgtgcag tgacgccacg ctgacggttg ggtcggcttc cattcgggac
      781 tgcctcggtg cgaaaaggac gccgattttg cccttgaacc cgccccgacc ttcacacgtg
      841 cggggagaac gcgatccctg cgatccctac aatgggccgc gatgaacaac ttcgatgtgc
      901 aggtgctcgg cgcgggcatc gtcgggcgct gcctcgcgct cgcgctcgcg cgtgccgggc
      961 tgagcgtcgc gctgcggccc gaggccgcgc gtgcgccggc cggcgacgac gtgcgcgcgt
     1021 acgcgctgaa cgcggcgtcg gtcgagttgt tgcgcgggct gaaggtctgg gatgcgctgc
     1081 ccgcgcacgc cgccacaccg gtgctcgaca tgcacgtgca gggcgacgcc cacgccgcgg
     1141 cgatcgactt ctcggcctgg cagcaacggg tcggcgcgct ggcctggatc accgacgccg
     1201 cggtgctcga gcgcgagctc gccgcggcgg tgcgcttcgc gccgcacatc acggtggtcg
     1261 acgccggcgc gcaggtgaac gccgcgctga ccgcgctgtg cgagggcaag gcctcggcga
     1321 cccgcgcggc gctgggcatc gcattcgagc gcgtcgacta cggccaccgc gcgatagcgg
     1381 cgcgcctgac cagcacgcgt gggcaccagg gcatggcccg gcagtggttt cgcgcacccg
     1441 atgtgctcgc gctgctgccg ttcgacacgc cgcagccgca gcactcgttc gcgctggtgt
     1501 ggtcgctgcc cgacgcgcgt gcgaacgagc tgctcgcact cgacgccgct gccttcgagg
     1561 ccgagctgat gcaggcgacc aagggcgagg ccggcacgct cacgctcgcc tccgagcgcg
     1621 ccgcgtggcc gctgatgcag gcgaacgcga gcgcctggtg cggcccgggc tgggtgctgc
     1681 tcggcgacgc cgcgcacgtc gtgcatccgc tcgccggcca gggcctgaac ctcggcctgg
     1741 ccgacgtcgc ggcgctggtc aaggtgatcc gcgaacgcga ggtctggcgc ccgctcggcg
     1801 acgcgaagct gctgcgccgc tacgcccgcg agcgcgtcgt gccgacctgg gcgatggggc
     1861 aggtgaccga cggcctgctg cgacttttcg ccgaagacgc gccggccgtg cgggaacttc
     1921 gcaaccgcgg cctgactctg gtgaacaagc tttccccgct gaagcgctgg ctcacggcgc
     1981 gggcactgaa ctcctgaaag atcgtcatga acacgctgct gcgcccgatc ctcgcgctga
     2041 ccctcggcct cgccttcgcc cacgcggcgc tcgccgacga ggcggcgatc cgcaagaacc
     2101 tgaccgagcg gctgcccgag ctgccgaaga tcgacgaggt cagcaagacc gcgatccccg
     2161 ggctgtacga ggtgcgcatc ggcaccgagc tgctctacac cgacgagcgc ggcgaccacg
     2221 tgatccaggg cagcatcgtc gagacagcga cccgcaccga cctcacgcag gcgcgcatcg
     2281 acaagatcac cgcgatcgat ttcgccgcgc tgccgttgaa ggacgcgatc gtctggaagc
     2341 agggcaccgg cgcgcgcaag ctggtcgtgt tcgccgaccc gaactgcggc tactgcaaga
     2401 agttcgagac cgagatgcag caggtcaaga acgtgacggt ctataccttc ctggtgccga
     2461 tcctgggcgg cgactcgccc gacaagtcgc gcaacatctg gtgcgcgaaa gacaacgcca
     2521 cggtctggcg cgactggatg atcaagggcg cgcccgcacc gcgcagcatg ggcaattgcg
     2581 acgtctcggc gctgcagcgc aacatcgcga tggcgaagaa gtaccgcgtc aacggcacgc
     2641 cggcgctggt cttcgaagac ggcaagcgcg tgccgggcgc gctgccaccg gatgaagtgg
     2701 agaagcggct gcttgccggc cgcggcgcca agggctgagc ggccggcgcg tcgcgcgcgc
     2761 cgaccagtcg agcgcgagga tccagaacgc cgacggcgtg gtgccgaagg cgacgaagaa
     2821 gctcgacgat agggcggcgc gcggcgtgcg cctcggatcg cggttcagac gctgcgggcc
     2881 acaacttgca gatcgaatta cagtgcgctc gccctcacac tgtccggagc tcgcgcccgg
     2941 cgcttcgatg ctcactttcg ccatcaccgc ctttgatccg cgcgcccatc tgttccgcgt
     3001 cagcgtgcgc attcccgccc ccgcggccga acagaggctg agcctgccgg cctggatccc
     3061 ggggagctac ctggtgcgcg agttcgcccg gcacctgtcg gggctgtcgg cgagccaggg
     3121 tcgccgcgcc gtgccgctgg cgcagctcga caaggccacc tggctggcgc gctgcgaggg
     3181 cggggcgacg ctgacggtca gctacctggt ctatgccttc gacacctccg tgcgggcggc
     3241 gtttctcgac gcccagcggg gtttcttcaa cggcacctcg gtgttcctgc gggtcgaggg
     3301 gcgcgcggcc gagccgcacc ggctcgaact gcgcggcctg ccggccggct ggcaggtcgc
     3361 cacggcactc acacccgtcg aggtcgacgc gttcgggcgc ggggtctacg cggcggccgg
     3421 ctacgacgaa ttggtcgacc acccggtcga gctcggccgc ttctggcgcg gccgcttcga
     3481 ggcgcacggc gtgccgcacg agtacgtggt cgcgggcgcg ctacccgact tcgacggcgc
     3541 gcggctgctc gccgacgccc agcgcatctg cgaagcgcag atcaggttct ggcacccatt
     3601 gggcaagccg ccgttcgagc gctatgtctt cctgcacaac ctggtcgacg aaggccacgg
     3661 cgggctcgag caccgcgcca gcacggcgct gatttcgccg cggcgcgaca tgccggcggt
     3721 tggcaagccg gactccagcg acggctacgt gaacgtgctc gggctggtca gccacgagta
     3781 cttccacacc tggaacgtca agcgcctgcg ccccgccgag ttcgccagcg tcgactacac
     3841 gcaggagaac tacaccgagc tgctgtggtt cttcgagggc ttcacctcgt actacgacga
     3901 cctgttcctg ctccgcaccg ggttgatcga tacggcacgc tacctgcggc tggttgcacg
     3961 caccgtcagt ggtgtgctgt cgatgcc
//
LOCUS       DASRDG010000107         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_475567, whole genome shotgun sequence.
ACCESSION   DASRDG010000107 DASRDG010000000
VERSION     DASRDG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475567"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFA35_01785"
     CDS             <1..314
                     /locus_tag="VFA35_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT54927.1"
                     /translation="YGDQRRLEVARALATDPKLIALDEPAAGMNATEKRTLRELIDRI
                     RNDGRTILLIEHDVKLVMGLCDRVTVLDYGKQIAEGTPAEVQRNPKVIEAYLGADHVA
                     H"
     gene            301..1062
                     /locus_tag="VFA35_01790"
     CDS             301..1062
                     /locus_tag="VFA35_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT54928.1"
                     /translation="MSPIEVGSALAAAAAPGMKTLLRVNGLKVAYGGIQAVKGVSFEV
                     HEGELVSLIGANGAGKTTTLKAITGTQAAAGGEIEFMGRSIRGQGPWDLVKQGLVMVP
                     EGRGTFTRMTITENLQMGAFVRNDKDIDADIDKVFAIFPRLKERRNQLAGTMSGGEQQ
                     MLAMGRALMARPKVLLLDEPSMGLSPIMVDKIFEVVADIHQRGTTVLLVEQNASRALQ
                     LASRAYVMDSGEVTMSGDAKQLLDDPKVRAAYLGE"
     gene            1252..2262
                     /locus_tag="VFA35_01795"
     CDS             1252..2262
                     /locus_tag="VFA35_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54929.1"
                     /translation="MSVTALSRTTAAPEPLLFRRLRLGAAEAAPAALGGRRVGQGPQA
                     LHAPLLAPAGAVFDAWASAAPAEARRHGCVQYATDGQWLHGFAELDDHIDGLQAASHR
                     VYRDLFAVLADSACPHLLRLWNYFADINREVGGVERYRQFNAGRQQAFIDAGRSAFEG
                     SPAACALGTHDGPLRVYFLAGRQPPLAIENPRQVSAYHYPETYGPRRPSFSRAALTDV
                     GGGRQALLISGTASIVDHKTVHVGDVRKQTEESLLNIAAVRQVAAERAGVAFPAGELI
                     CTVYVRDPADLDVVREVFEREVGAGSAAARDCVYLQADICRADLLVEIEAYGFARAGA
                     AA"
     gene            2259..>2977
                     /locus_tag="VFA35_01800"
     CDS             2259..>2977
                     /locus_tag="VFA35_01800"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HZT54930.1"
                     /translation="MIARGLVLAVAGALSVGACAAEQPVEPGPDVDAGLNPGVKRPLW
                     ELGLGVAGLRLPDYLGSDQSRFYALPLPYFVYRGTWLKADRDGARALLFDAQRVKVDV
                     SVAASTPARSRDNDARAGMPNLSGAAELGPNLNLTLASSPKDRWKLDLRLPLRGAVSL
                     ERSPKFVGTTFSPNLNLDIGGIGGGWNLGMLTGPVFADRKFHQHYYGVDPAYASANRA
                     AYQARGGYAGWRALAATSRRFG"
BASE COUNT          435 a         1108 c         1038 g          396 t
ORIGIN      
        1 cgtacggcga ccagcgccgc ctcgaggtgg cgcgcgcgct cgccaccgac ccgaagctga
       61 tcgccctcga cgagcccgcc gccggcatga acgcgaccga gaagaggacg ctgcgcgaac
      121 tgatcgaccg gatcaggaac gacggccgga cgatcctgct catcgagcac gacgtcaagc
      181 tcgtcatggg cctgtgcgac cgcgtcacgg tgctcgacta cggcaagcag atcgccgaag
      241 gcacgccggc cgaggtgcag cgcaacccga aggtgatcga ggcctatctc ggagccgacc
      301 atgtcgccca ttgaggtcgg atcggcgctg gcggcagccg ccgcgcccgg aatgaagacg
      361 ctcctgcgcg tcaacgggct gaaggttgcc tacggcggca tccaggcggt caagggcgtc
      421 tcgttcgagg tccacgaagg cgagctggtc agcctgatcg gcgccaacgg cgccggcaag
      481 acgacgacgc tgaaggccat caccggcacc caggcggcgg ccggcggcga gatcgagttc
      541 atgggccgca gcatccgcgg ccagggcccg tgggacctcg tcaagcaggg tctcgtgatg
      601 gtgcccgagg gccgcggcac cttcacccgc atgacgatca ccgagaacct gcagatgggc
      661 gcgttcgtgc gcaacgacaa ggacatcgat gccgacatcg acaaggtgtt cgcgatcttc
      721 ccgcgcctga aggagcgccg caatcagctc gccggcacga tgagcggcgg cgagcagcag
      781 atgctcgcga tgggccgcgc gttgatggcg cggcccaagg tgctgttgct cgacgagccg
      841 tcgatgggcc tgtcgccgat catggtcgac aagatcttcg aggtcgtggc cgacatccac
      901 cagcgcggca cgaccgtgct gctggtcgag cagaacgcga gccgtgcgct gcaactggcc
      961 tcgcgcgcct acgtgatgga ctcgggcgag gtgacgatga gcggcgacgc caagcaactg
     1021 ctcgacgacc cgaaagtgcg cgccgcctac ctcggtgagt agatggggcc ccggcacggg
     1081 gtaccgcgcc accccccgag ggggtccggg ccggcttggg agcggcccgg cgctcggccg
     1141 cggcgccgcc tcgacacctg ggttgcgacg atacaattcg ccggccatga accagcccct
     1201 gatcgcgcca tcgtgcgccc gttggccgcg tgaccgcgag ggcacgggcg cgtgagcgtc
     1261 accgccctgt cgcgcaccac cgccgcccct gaaccgctgc tgttccggcg cctgcggctc
     1321 ggtgcggccg aggccgcgcc cgccgcgctc ggcggccggc gcgtggggca gggcccgcag
     1381 gcgctgcacg cacccctgct cgcgccggca ggcgcggtgt tcgacgcttg ggccagcgcc
     1441 gcgcccgccg aagcgcgccg ccacggctgc gtgcagtacg cgaccgatgg ccagtggctg
     1501 cacggcttcg ccgagctcga cgaccacatc gacggcctgc aggccgcgtc gcaccgcgtc
     1561 taccgcgacc tgttcgcggt gctcgccgac agtgcctgcc cgcacctgct gcggctgtgg
     1621 aactacttcg ccgacatcaa ccgcgaggtc ggcggcgtcg agcgctaccg ccagttcaac
     1681 gccggccgcc agcaggcctt catcgacgcc ggccgcagcg ccttcgaagg ctcgccggcg
     1741 gcgtgcgcgc tgggcacgca cgacggcccg ctgcgcgtct acttcctggc cggccgccag
     1801 ccgccgctgg cgatcgagaa cccgcgccag gtcagcgcct accactaccc cgagacttac
     1861 ggaccgcgcc gcccgagctt ctcgcgcgcc gcgctcaccg atgtcggcgg cggccggcag
     1921 gcgttgctca tctcgggcac cgcgagcatc gtcgaccaca agacggtgca cgtcggcgac
     1981 gtgcgcaagc agaccgaaga gagcctgctg aacatcgcgg ccgtgcgcca ggtcgcggcc
     2041 gagcgcgccg gcgtggcgtt cccggccggc gaactgatct gcacggtgta tgtgcgcgac
     2101 ccggccgacc tcgacgtcgt gcgcgaggtc ttcgagcgcg aggtcggcgc cggcagcgcg
     2161 gcggcgcgcg attgcgtcta cctccaggcc gacatctgcc gcgccgacct gctggtcgag
     2221 atcgaggcct atggcttcgc gcgcgccgga gccgctgcgt gatcgcacgc ggcctggtgc
     2281 tcgcggtggc cggcgcgctc agcgtcggcg catgcgccgc cgagcaaccg gtcgagcccg
     2341 gccccgatgt cgacgccggt ctgaacccgg gtgtcaagcg cccgctgtgg gaactcggcc
     2401 tcggcgtcgc cggcctgcgc ctgcccgact acctcggttc cgaccagagc cgtttctacg
     2461 cgctgccgct gccctacttc gtctaccgcg gcacctggct gaaggccgac cgcgacggcg
     2521 cgcgcgcgct gctgttcgat gcgcagcgcg tcaaggtcga cgtgagcgtc gccgcatcga
     2581 cgcccgcgcg cagccgcgac aacgacgccc gcgccggcat gccgaacctg tcgggcgccg
     2641 ccgagctcgg tccgaacctg aacctcacgc ttgcgagttc gccgaaggac cgctggaagc
     2701 tcgacctgcg cctgccgctg cgcggcgcgg tctcgctcga gcgttcgccg aagttcgtcg
     2761 gcaccacgtt ctcgccgaac ctgaacctcg acatcggcgg catcggcggc ggctggaacc
     2821 tcggcatgct gaccggcccg gtgttcgccg accgcaagtt ccaccagcac tactacggcg
     2881 tcgaccctgc ctacgcgagc gcgaaccgcg ccgcgtacca ggcccgtggc ggctacgccg
     2941 gctggcgcgc gctcgccgcc acgtcgcgcc gcttcgg
//
LOCUS       DASRDG010000108         5243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_251949, whole genome shotgun sequence.
ACCESSION   DASRDG010000108 DASRDG010000000
VERSION     DASRDG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251949"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VFA35_01805"
     CDS             complement(<1..104)
                     /locus_tag="VFA35_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HZT54931.1"
                     /translation="MDIAGKVFIVTGGASGLGEGTARMLAGNGAKVVVA"
     gene            complement(119..772)
                     /gene="adk"
                     /locus_tag="VFA35_01810"
     CDS             complement(119..772)
                     /gene="adk"
                     /locus_tag="VFA35_01810"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZT54932.1"
                     /translation="MRLILLGAPGAGKGTQAAFICKKFGIPQISTGDMLRAAVKAGSP
                     LGIEAKKVMDSGALVSDDIIIGLVKERIAQPDCANGFLFDGFPRTIVQAEAMRAAGVK
                     LDLVLEIDVPDAAIIERMGGRRVHVASGRTYHLKFNPPKVTGKDDVTGEDLIQRADDE
                     EATVRNRLAVYQNQTRPLVDYYSTWAASGDANAPRHCKISGLGSVDEITARALAALG"
     gene            complement(917..1678)
                     /gene="kdsB"
                     /locus_tag="VFA35_01815"
     CDS             complement(917..1678)
                     /gene="kdsB"
                     /locus_tag="VFA35_01815"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZT54933.1"
                     /translation="MKFTVLVPARLASTRLPNKPLADIAGLPMIVRVARNAARSAAQR
                     VVVAADDARIVDACAAHGVDALLTRDDHATGSDRLAEACALLGLDGADLVVNVQGDEP
                     LLDPQMIDACAALLAQHPDCVMSTAAHPIDTIDELDNPNVVKVVLDAAGRALYFSRAP
                     IPWARDARAAGLVAMPADAPLRHIGLYAYQAGFLRRFPGLAASPLERIEALEQLRVLW
                     HGERIAVHVSDSRPGPGVDTPDDLVRVRALFASLS"
     gene            complement(1675..1872)
                     /locus_tag="VFA35_01820"
     CDS             complement(1675..1872)
                     /locus_tag="VFA35_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HZT54934.1"
                     /translation="METRLLDLLVCPICKGPLEHLRPPQQANVELVCHADGLAFPVRD
                     GIPVMLEAEARPLDAATATAP"
     gene            complement(1853..2899)
                     /gene="lpxK"
                     /locus_tag="VFA35_01825"
     CDS             complement(1853..2899)
                     /gene="lpxK"
                     /locus_tag="VFA35_01825"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZT54935.1"
                     /translation="MAAPRPFAQRLPQVWLARGPLACALLPLALVYGAFMACRRALYR
                     LGVLRAQVLTVPVIVVGNLVAGGAGKTPTTIAIVAMLRRHGYTPGIVSRGYGGSSDGP
                     LEVTRAVPARQCGDEPLLLHLRTGAPAVIGRDRVGAALELLRRHPAVDVVVSDDGLQH
                     LALARDVQVIVFDERGAGNGWLLPAGPLREPLPRALPARSLVVYNAAAASTPLPGTLA
                     RRALTGCVALAAWWRGEAASSAALEALRGRPLLAAAGLARPARFFEMLRARGLTIVPL
                     PLADHHDFAALPWPTDAPDVVLTEKDAVKLDPARIGSRVWVAPLDFTLDAAFEAALIA
                     LLAPPGPRHGNPPP"
     gene            complement(2920..3348)
                     /locus_tag="VFA35_01830"
     CDS             complement(2920..3348)
                     /locus_tag="VFA35_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZT54936.1"
                     /translation="MNFRKARPEEPEINLIPFIDVLLVVLIFLMLSTTYSKFTELQIT
                     LPVADAEKLRDRQREVIVAVSGDGRYAVNRKPVEGRSVDTLTAELVAAAAGATDAVVI
                     VSADATAAHQSVINVMDAARRANLPRLTFATQTSSGGEAR"
     gene            complement(3365..4003)
                     /locus_tag="VFA35_01835"
     CDS             complement(3365..4003)
                     /locus_tag="VFA35_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZT54937.1"
                     /translation="MFSIIQAAGWPIWPLLLCSVGAFALVLERLSSLRTARVVPPKLL
                     DEVISVTRAHLPPADVVNKLADNSVLGGVLASGLRAVIAEPRISERQLRDTFESAGRA
                     AVHRLERYLNTLGTIASAAPLLGLMGTVIGMIEIFGSQAPTGNNPALLAHGISVALYN
                     TAFGLMIAIPSLMFYRYFRGRVDGYTLDMEQASDRLVPHLMRFVVDRRQSSN"
     gene            4382..>5243
                     /gene="xseA"
                     /locus_tag="VFA35_01840"
     CDS             4382..>5243
                     /gene="xseA"
                     /locus_tag="VFA35_01840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZT54938.1"
                     /translation="MVDGFGASPSRGAWGVAALVREVGDLLAGRFSVCTVRGEISGFA
                     RAASGHCYFNLKDADGGSALLRCAMFRRAAGLLGFAPHDGQLVELRGRLGVYEPRGEL
                     QLVVEAMQGAGAGALYEQFLRIRARLEAQGLFDPARKRALPAYPRAVGVVTSLGAAAL
                     HDVLTALARRAPQVRVVVYPSLVQGAAAPAALAEAIALAGRRAEVDALIVCRGGGSLE
                     DLWAFNDEAVVRAIVAAPMPVVCGVGHETDLTLADLCADLRAPTPTAAAELVAPTRDA
                     CLNVLTAFAAL"
BASE COUNT          729 a         1783 c         1975 g          756 t
ORIGIN      
        1 gcgacgacga ccttcgcgcc gttgccggcc agcatgcggg ccgtgccttc gccgagaccc
       61 gacgcgccgc cggtgacgat gaagaccttg cccgcgatgt ccatgcgtcg cgcgcgcttc
      121 agccgagcgc ggcgagcgcc cgcgcggtga tctcgtcgac gctgcccagg ccgctgatct
      181 tgcaatggcg cggcgcattg gcatcgccgc tggcggccca ggtcgagtag tagtcgacca
      241 gcggtcgggt ctggttctgg tagaccgcga ggcggttgcg caccgtcgcc tcttcgtcgt
      301 cggcgcgctg gatcaggtct tcgccggtca cgtcgtcctt gcccgtgacc ttcggcgggt
      361 tgaacttgag gtggtaggtg cggcccgacg ccacgtgcac gcggcggccg cccatgcgct
      421 cgatgatggc cgcgtcgggc acgtcgatct cgagcacgag gtcgagcttg acgccggcgg
      481 cgcgcatggc ctcggcctgc acgatcgtgc gcgggaagcc gtcgaacaga aagccgttgg
      541 cgcaatcggg ctgcgcgatg cgctccttga cgaggccgat gatgatgtcg tcgctgacga
      601 gtgcgcccga atccatcacc ttcttcgcct cgatgccgag cgggctaccg gccttgacgg
      661 ctgcgcgcag catgtcgccg gtcgatatct gcgggatgcc gaacttcttg cagatgaagg
      721 cggcttgggt gcctttgccc gcgcccggcg cgcccagcag gatgagtctc atgggtgtcc
      781 tcgttctcgt ctcgtgtgtg agccgcgcac ggcgatggca gcgggcgcga atcgaatcga
      841 gaataacacg tggctctcgc acgaagctga cgcgcgggca cgggaaaaca cgtgctgcgt
      901 gcggcctcgg atcaggtcag gagagcgacg cgaagagcgc tcgcacgcgc accaggtcgt
      961 cgggcgtgtc gacacccggc ccgggccgcg aatcgctgac gtgcaccgcg atgcgttcgc
     1021 cgtgccagag cacgcgcagt tgctcgagtg cctcgatgcg ttcgagcggg ctcgcggcga
     1081 ggcccggaaa gcgccgcagg aacccggcct ggtacgcgta caggccgatg tgccgcagcg
     1141 gcgcgtcggc gggcatcgcc accaggcccg cggcccgcgc atcgcgcgcc cacgggatcg
     1201 gcgcgcgcga gaaatagagc gcacggccgg cggcgtcgag cacgaccttg acgacattcg
     1261 ggttgtcgag ctcgtcgatc gtgtcgatcg ggtgcgcggc ggtgctcatc acgcagtcgg
     1321 ggtgctgcgc gagcagcgcg gcgcaggcgt cgatcatctg cgggtcgagc agcggttcgt
     1381 cgccctgcac gttgacgacc aggtcggcgc cgtcgaggcc gagcagcgcg caggcttcgg
     1441 cgaggcgatc gctgccggtg gcgtggtcgt cgcgcgtgag cagcgcgtcg acgccgtgcg
     1501 ccgcgcaggc atcgacgatg cgcgcgtcgt cggccgcgac gaccacccgc tgcgccgccg
     1561 agcgcgcggc gttgcgcgcc acgcgcacga tcatcggcag gccggcgatg tcggcaagcg
     1621 gcttgttcgg cagccgggtc gacgccaggc gtgccggcac gagcaccgtg aacttcacgg
     1681 ggcggtggcg gtcgcggcgt cgagcgggcg cgcctcggct tcgagcatca ccgggatgcc
     1741 gtcgcgcacc gggaaggcca ggccgtcggc atggcagacg agctcgacgt tcgcctgctg
     1801 cggcgggcgc aggtgttcga gcggaccctt gcagatcggg cagacgagca ggtcaaggag
     1861 gcgggtttcc atggcggggt ccgggtggtg cgagcagggc gatcagcgcg gcctcgaagg
     1921 cggcgtccag ggtgaagtct agcggcgcca cccacacgcg cgagccgatg cgcgcggggt
     1981 cgagcttgac cgcatccttc tcggtcagca cgacatcggg ggcgtcggtg ggccaaggca
     2041 gcgcggcgaa gtcgtggtga tcggcgagcg gcagcggcac gatcgtgagg ccgcgcgcgc
     2101 gcagcatctc gaagaatcgc gccgggcgcg cgaggccggc cgcggcgagc agcggacggc
     2161 cgcgcaaggc ctcgagcgcc gcgctgctcg cggcctcgcc gcgccaccag gcggccagcg
     2221 ccacgcagcc ggtcagcgca cgccgcgcga gcgtgccggg cagcggtgtc gatgccgccg
     2281 cggcgttgta gacgaccagg ctgcgcgccg gcagcgcacg cggcaacggt tcgcgcagcg
     2341 gcccggccgg cagcagccag ccgttgccgg cgccgcgctc gtcgaacacg atcacctgca
     2401 cgtcgcgcgc cagcgccagg tgctgcagcc cgtcgtcgct gacgaccacg tcgaccgccg
     2461 gatgccggcg cagcagttca agcgccgcgc cgacgcggtc acggccgatc accgccggcg
     2521 cgcccgtgcg caggtgcagc agcagcggtt cgtcgccgca ctggcgtgcc ggcaccgcgc
     2581 gtgtcacctc cagcgggcca tcggagctgc cgccgtagcc gcgcgaaaca atgccgggcg
     2641 tgtagccgtg ccggcgcagc atcgcgacga tcgcgatcgt ggtcggcgtc ttgccggcgc
     2701 cgccggccac caggttgccg acgacgatca ccggcaccgt cagcacctgc gcgcgcagca
     2761 cgccgagccg gtacaacgcg cgccggcacg ccatgaacgc gccgtacacg agcgccagcg
     2821 gcagcagcgc gcaggcgagc gggccgcgcg cgagccagac ctgcggcagg cgctgcgcga
     2881 aggggcgggg cgcggccaag acgctgcggc gcggtcgctt cagcgcgctt cgccgcccga
     2941 cgaggtctgg gtcgcgaagg tcagccgggg caggttcgcg cgccgcgcgg cgtccatcac
     3001 gttgatcacg ctctggtgcg cggcggtcgc gtcggccgag acgatcacga ccgcgtcggt
     3061 cgcgccggcg gcggcggcca ccaactcggc cgtcagcgtg tcgacgctgc gcccctcgac
     3121 cggcttgcgg ttcaccgcat agcggccgtc gcccgagacc gcgacgatca cctcgcgctg
     3181 gcggtcgcgc agcttttcgg cgtccgccac cggcagcgtg atctgcagct cggtgaactt
     3241 cgagtaggtg gtcgacagca tcaggaagat cagcacgacg agcagcacgt cgatgaacgg
     3301 gatcaggttg atctcgggtt cctcgggccg cgccttgcgg aagttcatcg gcatcgcgcg
     3361 ggcctcagtt gctgctctgg cggcggtcga cgacgaagcg catcaggtgc ggcacgagcc
     3421 ggtcggaggc ctgctccatg tcgagcgtgt agccgtcgac gcggccgcgg aagtagcggt
     3481 agaacatcag cgacgggatc gcgatcatca gcccgaacgc ggtgttgtac agcgccaccg
     3541 agatgccgtg ggcgagcagc gccgggttgt tgccggtggg ggcctgcgac ccgaagatct
     3601 cgatcatgcc gatcaccgtg cccatcagcc cgagcaaggg ggcggcagac gcgatcgtgc
     3661 ccagcgtgtt caggtagcgc tcgagccggt gtaccgcggc gcggccggcc gactcgaagg
     3721 tgtcgcggag ctggcgctcg ctgatgcgtg gctcggcgat cacggcccgc aggccgctgg
     3781 ccagcacgcc gcccagcacc gagttgtcgg cgagcttgtt gaccacgtcg gccggcggca
     3841 ggtgggcgcg cgtcacggag atcacctcgt cgagcagctt cggcggcacg acccgcgcgg
     3901 tgcgaaggct ggacaggcgc tcgagcacca gggcgaaggc cccgacggaa cacagcagga
     3961 ggggccagat cggccagccg gcggcttgta tgatcgaaaa caatcgtgcc ctttcgacgg
     4021 tgctgtggtg gcggcgaggg gatttcggga cgcgcgatta tggcctgaag gtgcgcccct
     4081 cacgcacccg cgacggcggt gttgcggccc ggcgcaaccg ccgcgccgct cggcgccgtg
     4141 aatcccgtcc cggacatcca ccgcatttgt ggataacttt gtgggcaacc gggtgcggcc
     4201 cgccgcgaag gcccgaatct gcgaggcccg agacacattg cttaaagttt aggcagatag
     4261 aaaaccttga aaatcaacaa cttacgtgtt tgttacgggc ttgtgacagg gtcaatggcc
     4321 ggcgcgccct tgtgtggcgc ggttgtggag ttctcgaccc gcaccggcct tgggtttgcg
     4381 cgtggttgac ggcttcggtg cgagcccgtc gcgtggcgcc tggggcgtgg ccgcgctggt
     4441 gcgcgaggtc ggcgacctgc tggcgggccg cttcagcgtg tgcacggtgc gcggcgagat
     4501 ctcgggcttc gcgcgggcgg cgagcggcca ctgctatttc aacctgaaag atgccgacgg
     4561 cggcagcgcg ttgctgcgct gcgcgatgtt ccggcgcgcc gccggcctgc tcgggttcgc
     4621 gccgcacgac gggcaactcg tcgagttgcg cgggcggctt ggcgtgtacg agccgcgcgg
     4681 cgaactgcaa ctggtcgtcg aggcgatgca gggtgcgggt gccggcgcgc tttacgagca
     4741 attcctgcgc atccgggcgc ggctcgaagc gcaaggcctg ttcgacccgg cgcgcaagcg
     4801 ggcgctgccg gcgtatccgc gcgcggtggg tgtcgtcacc tcgctcggcg cagcggcgct
     4861 gcacgacgtg ttgacggcgc tggcgcggcg tgcgccacag gtgcgcgtcg tcgtctaccc
     4921 gagcttggtg cagggcgcgg cggcaccggc tgcgctggcc gaggcgattg cgctcgccgg
     4981 gcgccgcgcc gaggtcgacg cgctgatcgt ctgccgcggc ggcggttcgc tcgaggacct
     5041 gtgggccttc aacgacgaag cagtcgtgcg ggcgatcgtc gcggcgccga tgccggtcgt
     5101 ttgcggcgtc ggccacgaga ccgacctgac gctggccgac ctctgcgccg atctgcgcgc
     5161 gccgacgccg accgccgccg ccgagctcgt cgccccgacc cgcgacgcct gcctgaacgt
     5221 gctcactgcg ttcgccgcgt tgc
//
LOCUS       DASRDG010000109         3507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_374280, whole genome shotgun sequence.
ACCESSION   DASRDG010000109 DASRDG010000000
VERSION     DASRDG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374280"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFA35_01845"
     CDS             <1..371
                     /locus_tag="VFA35_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HZT54939.1"
                     /translation="ITACAEVRALAEVYALQVYYREPFADEDSMPAAAHAAWLAWYEH
                     TPDAPCIAICSTSQGDDVCKGCGRTFDEVQHWPTMTPSEKRQTWRRITMDGSAWRFNK
                     YAERAHEAVPATVPPELTPR"
     gene            complement(358..1677)
                     /locus_tag="VFA35_01850"
     CDS             complement(358..1677)
                     /locus_tag="VFA35_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HZT54940.1"
                     /translation="MPAKPFDLIVFGATGFTGRQAVRALVHRAAAESTHTPLRWAVAG
                     RNGERLRAVVAEQVPAGAAQPGVVIADADQPDTLHALAAQTSVLLNLAGPYALTGEAV
                     VQACIAQGTHHLDLAGETFWVRQLIERHHRAAQRAKVKVIVCCGYEALPFDLAALWAA
                     QQLRQRSGEACRELKLVVRFTGKRITSPKDAVSGGTIGSMRMLLELDTTDCVRNPACL
                     LPPDAADAAAIAARNAYRFVPQYDDDVHAVTAPTIPAPFINPPIVLRSVALAQDASLF
                     APDFRYVESTDMASLVPQPAFVPRAATLPLQWAAAASIAAPLAALSASVAGPLAFQRG
                     ALRRLIELFAPAPGAGPSEAVLAESGYVFEVFATSASGQRFHGRVTAQGHPGYRSTPE
                     MVVTAGIGLARGELGRTPHFGIVTPATGLGLEAVEALREAGVVFSAA"
     gene            complement(1740..3098)
                     /locus_tag="VFA35_01855"
     CDS             complement(1740..3098)
                     /locus_tag="VFA35_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZT54941.1"
                     /translation="MKNLYLRIYVTVVVVLLLFAAASGWVFQRHMEQERVRAEAVLTE
                     RMGAWAELIERSLPGQDQPAADQAAALLEWSQRLRLPLALDSDKGVRIAQSESFARRQ
                     TEGGPVRGLAVRLDDGRTLWIMRPGLRQGGGRLGLGGPPPGARPGGPDGPGGPPPFLP
                     FVPPAWQQGLGLVVVLVILFIAVAAGAYPVVRRLTRRLEALKLGVEQFGAGQLSHRVQ
                     VTGHDEVAAVASSFNVAAARVEALVRSHQSLLANASHELRSPLARMKMAVSMLEDAPP
                     LQRALLKKEIDTNVGELDALVEEVLLASRLDAAATPERQERVELLGMAAEEAARVDAA
                     VDGAPTTVLGEDRLIRRALRNLLENARRYGGGEVEVIVGVSGGRAEVRVCDRGPGVPE
                     AMRERIFEPFFRLPGHAEQAGGVGLGLSLVKQIAERHGGSVRCEARAGGGSCFVLALP
                     LA"
     gene            complement(3136..>3507)
                     /locus_tag="VFA35_01860"
     CDS             complement(3136..>3507)
                     /locus_tag="VFA35_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZT54942.1"
                     /translation="KALLRRASPEPVNEEVLRFGKLEIDLGAHEARLDGVRCDLTSHQ
                     FDLLVVLAQSPGRVLSRDQIMDSLKGHPLEAFDRSIDVHISRIRAVIEDDPKLPRRVL
                     TVRGAGYLFARKQDAGESPES"
BASE COUNT          504 a         1311 c         1263 g          429 t
ORIGIN      
        1 gcatcaccgc gtgcgccgag gtgcgcgcgc tggccgaggt gtatgcgctg caggtctact
       61 accgcgagcc cttcgccgac gaggattcga tgccggccgc cgcgcacgcc gcctggctcg
      121 cctggtacga acacacgccc gacgcgccct gcatcgcgat ctgctcgacg agccagggcg
      181 acgacgtctg caagggctgc ggccgcacct tcgacgaggt ccagcactgg ccgacgatga
      241 cgccgagcga gaagcgccag acctggcgcc gcatcacgat ggacggcagc gcctggcgct
      301 tcaacaagta cgccgagcgg gcgcacgaag ccgtgccggc aaccgtgccg cccgaactca
      361 cgccgcgctg aacacgacgc cggcttcgcg cagtgcctcg accgcctcca gccccaggcc
      421 ggtggcgggc gtgacgatgc cgaaatgcgg cgtgcggccg agctcgccgc gcgccaggcc
      481 gatgccggcg gtgaccacca tctcgggcgt cgagcgatag cccgggtggc cttgcgccgt
      541 gacgcggccg tgaaagcgct ggccggacgc cgaggtcgcg aacacctcga acacatagcc
      601 gctttcggcc agcaccgcct cgctcgggcc ggcgccgggt gcgggtgcga acagctcgat
      661 cagccggcgc agcgcgccgc gctggaacgc cagcgggcct gccaccgagg cgctcagcgc
      721 cgccagcggc gcggcgatcg acgccgccgc cgcccactgc agcggcagcg tcgcggcacg
      781 cggcacgaac gcaggctgcg gcaccaggct cgccatgtcg gtcgactcga catagcggaa
      841 gtcgggcgcg aacaggctcg cgtcctgcgc cagcgcgacc gagcgcagca cgatcggcgg
      901 gttgatgaac ggcgcgggaa tcgtcggcgc ggtgacggcg tgcacatcgt cgtcgtactg
      961 cgggacgaag cgatacgcgt tgcgcgcggc gatcgcggcc gcgtcggcgg catccggcgg
     1021 cagcaggcag gccgggttgc gcacgcagtc ggtggtgtcg agttcgagca gcatgcgcat
     1081 cgagccgatg gtgccgccgc tgaccgcgtc cttcgggctg gtgatgcgct tgccggtgaa
     1141 gcgcacgacc agcttgagtt cgcggcaggc ctcgccgctg cgctgccgca gctgctgcgc
     1201 cgcccacagc gcggccaggt cgaacggcag ggcctcgtag ccgcagcaga cgatgacctt
     1261 gaccttggcg cgctgcgcgg cgcggtggtg gcgctcgatc agctggcgca cccagaaggt
     1321 ctcgccggcg aggtcgaggt ggtgcgtgcc ctgcgcgatg caggcctgca ccacggcttc
     1381 gccggtcagc gcgtacgggc cggccagatt cagcagcacg ctggtctgcg ccgccagcgc
     1441 gtgcagcgtg tcgggctggt ccgcgtcggc gatcacgacg ccgggctgcg ccgcaccggc
     1501 gggcacctgc tcggccacga ccgcgcgcag ccgttcgccg ttgcgccccg ccaccgccca
     1561 gcgcagcggc gtgtgcgttg actcggcggc cgcgcgatgc accagcgccc gcaccgcctg
     1621 gcggccggtg aagccagtgg caccgaagac gatcaggtcg aagggcttgg caggcatggc
     1681 gcgatcatcg cgatggatcg cccagcttag cgcagccacc gcgccaccga cagcgcggat
     1741 caagccagcg gcaacgccag cacgaaacag ctcccgccgc ccgcgcgcgc ctcgcagcgc
     1801 acgctgccgc cgtggcgttc ggcgatctgc ttgaccaggc tcaggccgag gcccacgccg
     1861 ccggcctgct cggcatggcc cggcaggcgg aagaacggct cgaagatgcg ctcgcgcatt
     1921 gcctcgggca cacccggccc gcggtcgcag acgcgcacct cggcgcgacc gcctgagacg
     1981 ccgacgatca cctcgacctc gccgccgccg tagcggcgcg cgttctcgag caggttgcgc
     2041 agcgcgcgcc ggatcaggcg gtcttcgccg agcaccgtgg tcggcgcgcc gtcaacggcg
     2101 gcgtcgaccc gcgccgcctc ttcggcggcc atgccgagca actcgacgcg ctcctggcgc
     2161 tcgggcgtcg ccgcggcgtc gagccggctc gcgagcagca cctcctcgac cagcgcgtcg
     2221 agctcgccga cattggtgtc gatctccttc ttgaggagcg cgcgctgcag cggcggcgcg
     2281 tcttcgagca tcgacaccgc catcttcatg cgcgccagcg gcgaacgcag ctcgtggctg
     2341 gcgttggcca gcagcgactg gtgcgagcgc accagcgcct cgacccgggc cgccgcgacg
     2401 ttgaagctcg acgcgaccgc ggcgacctcg tcgtgcccgg tgacctgaac gcgatgactc
     2461 aactggcccg cgccgaactg ctcgacgccg agcttcagcg cctcgagccg gcgcgtcagg
     2521 cgccgcacga ccggatacgc gccggccgcg accgcgatga acaggatcac gagcaccacg
     2581 accaggccga ggccttgctg ccacgcgggc ggaacgaacg gcaggaacgg cggcgggccg
     2641 cccggcccgt cgggaccgcc cggccgcgcg cccggcggcg gcccaccgag gccgagccgg
     2701 ccgccgcctt gccgcagccc cggccgcatg atccacagcg tgcgcccgtc gtcgagccgc
     2761 accgcgaggc cgcgcaccgg gccgccttcg gtctggcgcc gcgcgaacga ctcggactgc
     2821 gcgatgcgca cgcccttgtc gctgtcgagc gcgagcggca ggcgcagccg ctgcgaccac
     2881 tcgagcagcg cggcggcctg gtccgcggca ggctggtctt gaccgggcag cgagcgttcg
     2941 atcagttcgg cccaggcgcc catgcgctcg gtcagcacgg cctcggcgcg tacccgttcc
     3001 tgctccatgt ggcgctggaa cacccagccc gacgcggccg cgaacagcag caacaccacc
     3061 accacggtga cgtagatccg caggtagagg ttcttcaagc gcgagcgccc cgcgacgacg
     3121 ccgcgccgcc gcgcatcagg actcggggct ctcgccggcg tcctgcttgc gcgcgaacag
     3181 gtagccggcg ccgcgcacgg tgagcacgcg gcgcggcagc ttcgggtcgt cctcgatcac
     3241 ggcgcggatg cgcgagatgt ggacgtcgat cgagcggtcg aaggcttcga gcggatggcc
     3301 cttgagcgaa tccatgatct ggtcgcgcga gagcacccgc ccggggcttt gcgcgagcac
     3361 gacgagcaga tcgaactggt ggctggtgag gtcgcagcgc acgccgtcca ggcgcgcctc
     3421 gtgggcgccc aggtcgatct cgagcttgcc gaagcgcagc acttcctcgt tgaccggctc
     3481 ggggctggcg cggcgcagca gcgcctt
//
LOCUS       DASRDG010000110         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_590677, whole genome shotgun sequence.
ACCESSION   DASRDG010000110 DASRDG010000000
VERSION     DASRDG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590677"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1683)
                     /locus_tag="VFA35_01865"
     CDS             complement(229..1683)
                     /locus_tag="VFA35_01865"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HZT54943.1"
                     /translation="MLKAPVPIAIPVALSCALSFPLSFACAQTTEDAAAKAPASRTPA
                     LRDAVQKAIAWIGRQAVPVEGHEDAVTFPGTAEQPDRRSAIVYGGGAGVLIFLENAAK
                     VLGDEHVRALADRTANGLLATARKDEHGRTWVDPKMREGAAALYVGDAGVGAAFLARA
                     RLRGDAAALLVATEVGDALLARAQRDGDALGWDHQVEVIYGTAGTALFLLELAAAVPD
                     GARFRDGALAAGRHLIASSKSDDKEPGRRYWRWALAGNATYTGFSHGTAGVAYALLRI
                     GLECKDDACVQAAKDGAEWLQALAVRDGELVHWPVAPKSSTSMGGWCHGPPGTARLFL
                     LLYGATKEQRWLDTALGSARWVMAQAGPADAKIPPAFPPSFCCGVAGAVDFFCDLQRA
                     TGDAQYGAFAARAADYLLAKAEADGDGIKWANGVNDQKAAAHLHSVDLMLGASGEAFA
                     LLRVLTLGDAADPVVGLPDRRLAPAPAPVTTTGK"
     gene            complement(1787..>2835)
                     /locus_tag="VFA35_01870"
     CDS             complement(1787..>2835)
                     /locus_tag="VFA35_01870"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HZT54944.1"
                     /translation="RLEFENRNAGPVNLAVTIATTNAVHPQPWGYFTAIHCAGTTVTG
                     QTFQGPSITGARGTLRGLMLEDGADNTGRIPDLLLNHLEGDLCIRINGNRGDDHTFDG
                     TETAIGRWGWYFSPVDQPFAQDTSFQTSWALRWLPDNQHLELRRLMGSLFVFDPVHFV
                     NGISIALEHGIQNTSNADYALTAFLYVQHGGARNTLAAIDVGDPVSESANQVQFTQVA
                     MTPRTAGFLRDNFFGTGPVTDTVRQVRDFLQFRILPTTRTRALGFGMRLWRPATSSGA
                     CQADVLVDGHPAGLLHCFTHGTTCPWKEGGECEVELPHALTDGKNNMTVELRPRPGSD
                     PLMIARIWVYEYVK"
BASE COUNT          352 a         1039 c         1046 g          398 t
ORIGIN      
        1 ggatcgcggc gacggtggcc gcgtcgagca ccggcaggtc gtcgacagtg gcgaccgtcg
       61 gcttctgcgc ggcgaggccg gcggcgatcc acgggagaac ggacgcagag agaaagcgcg
      121 ggagtcttgg catgggcgtc cccggcccgg gtcggcgggc cgcggcgacg ggatcctagc
      181 gaacggcgcc ggccgcgccg cctgttcgcc atctcgttgc accagccgtc acttcccggt
      241 cgtcgtcacc ggcgcgggtg cgggcgcgag gcggcgatcg ggcagcccca ccaccggatc
      301 cgccgcgtcg ccgagcgtca gcacccgcag cagcgcgaac gcctcgccgc tggcgccgag
      361 catcaggtcg acgctgtgca ggtgggccgc tgccttctgg tcgttgacgc cgttcgccca
      421 cttgatcccg tcgccgtcgg cctcggcctt ggccagcagg tagtcggcgg cgcgcgcggc
      481 gaacgcgccg tactgcgcgt cgccggtggc gcgctgcagg tcgcagaaga agtcgaccgc
      541 gccggcgacg ccgcagcaga agctcggcgg gaacgccggc gggatcttcg cgtccgcggg
      601 cccggcctgc gccatcaccc agcgcgccga ccccagcgcc gtgtcgagcc agcgctgctc
      661 cttggtcgcc ccgtacagca gcaggaacag gcgcgccgtc cccggcgggc cgtggcacca
      721 gccgcccatg ctcgtgctgc tcttcggcgc gacgggccag tgcacgagct cgccgtcgcg
      781 caccgccagc gcctgcagcc actcggcgcc gtccttcgcc gcctgcacgc aggcgtcgtc
      841 cttgcactcg aggccgatgc gcagcagcgc gtaggcgacg cccgcggtgc cgtggctgaa
      901 gcccgtgtac gtcgcgttcc cggcgagcgc ccagcgccag tagcgccgcc ccggctcctt
      961 gtcgtcgctc ttgctgctcg cgatcaggtg gcggcccgcc gcgagcgcgc cgtcgcggaa
     1021 gcgcgcgccg tcgggcaccg cggcggcgag ctcgagcagg aacagcgccg tgcccgcggt
     1081 gccgtagatg acctcgacct ggtggtccca gccgagcgcg tcgccgtcgc gctgcgcgcg
     1141 cgcgagcagc gcgtcgccga cctcggtcgc gaccaggagc gccgcggcgt cgccgcgcag
     1201 acgggcgcgg gcgaggaacg ccgcgccgac gccggcgtcg ccgacgtaga gcgccgccgc
     1261 gccctcgcgc atcttcgggt ccacccaggt gcgaccgtgc tcgtccttgc gcgcggtcgc
     1321 gagcaggccg ttcgcggtgc ggtccgcgag cgcacgcacg tgctcgtcgc cgagcacctt
     1381 ggccgcgttc tcgaggaaga tcagcacgcc ggcgccgccg ccgtagacga tcgcggaccg
     1441 ccggtccggc tgctccgcgg tgcccgggaa cgtcaccgcg tcctcgtgcc cctcgaccgg
     1501 caccgcctgc cgcccgatcc acgcgatcgc cttctggacc gcgtcgcgca gtgcgggtgt
     1561 cctggacgcc ggcgccttcg cggccgcatc ctcggtcgtc tgcgcgcagg cgaacgagag
     1621 cgggaacgag agcgcgcacg agagcgcgac cggaatcgcg atcgggactg gtgctttgag
     1681 catcccctct tcgtacgcgc gcgccggcgg cgcgcgagcg ccgcaccggt tttcgcggcc
     1741 gggatccggg cgcacgcggg ccattcgccc ggtcgctcca aggccgtcac ttcacgtact
     1801 cgtacaccca gatgcgcgcg atcatcagcg ggtcgctgcc gggccgcggc cgcagctcca
     1861 ccgtcatgtt gttcttgccg tccgtcagcg cgtgcggcag ctcgacctcg cactcgccgc
     1921 cttccttcca cggacaggtc gtgccgtgcg tgaagcagtg caggaggccc gccggatggc
     1981 cgtccaccag cacgtcggcc tgacaggcgc cgctgctcgt cgccggccgc cagaggcgca
     2041 tgccgaagcc gagcgcgcgc gtgcgggtcg tcggcaggat gcggaactgc aggaagtcgc
     2101 gcacctgccg cacggtgtcg gtgaccgggc cggtgccgaa gaagttgtcg cgcaggaagc
     2161 cggcggtgcg cggcgtcatg gccacctgcg tgaactgcac ctggttggcc gactcgctga
     2221 ccggatcgcc gacgtcgatc gcggcgagcg tgttgcgggc gccgccgtgc tgcacgtaga
     2281 ggaacgccgt cagcgcgtag tctgcgttgc tggtgttctg gatgccgtgc tcgagcgcga
     2341 tcgagatgcc gttgacgaag tgcaccgggt cgaacacgaa cagcgagccc atcagccgcc
     2401 ggagctccag gtgctggttg tccggcagcc agcgcagcgc ccagctggtc tggaacgacg
     2461 tgtcctgcgc gaacggctgg tcgacggggc tgaagtacca gccccagcgg ccgatcgcgg
     2521 tctccgtgcc gtcgaacgtg tggtcgtcgc cgcggttgcc gttgatgcgg atgcagaggt
     2581 cgccctcgag gtggttcagc agcaggtcgg ggatgcggcc cgtgttgtcg gcgccgtcct
     2641 cgagcatcag cccgcgcagc gtgccgcgcg cgccggtgat cgacgggccc tggaacgtct
     2701 ggccggtcac cgtcgtgccg gcgcagtgga tcgcggtgaa gtagccccac ggctgcggat
     2761 gcacggcgtt ggtcgtcgcg atggtgaccg cgaggttcac cgggccggcg ttgcggttct
     2821 cgaactcgag ccgcg
//
LOCUS       DASRDG010000111         3800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_820921, whole genome shotgun sequence.
ACCESSION   DASRDG010000111 DASRDG010000000
VERSION     DASRDG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820921"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /gene="mreD"
                     /locus_tag="VFA35_01875"
     CDS             complement(<1..302)
                     /gene="mreD"
                     /locus_tag="VFA35_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZT54945.1"
                     /translation="MIMPRASDQLLLPVNPLFVAFTLLVAFALNIVPLGRVPMLPDFV
                     ALVLVFWNVHQSRRVGVGVAFAFGLLMDVHSGAVLGQHALAYTLLSYFAVTIHRRLL"
     gene            complement(299..1258)
                     /gene="mreC"
                     /locus_tag="VFA35_01880"
     CDS             complement(299..1258)
                     /gene="mreC"
                     /locus_tag="VFA35_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZT54946.1"
                     /translation="MAIGTIDRTPPPFFRQGPSALTKLILCAALALFLMVADNRFALT
                     RQLRAAVATALYYPQQALLVPVQAVRGGGEYLLGLQGALANEAAAKRALAQQSERALR
                     VEQLLAENAHLRGLLALRPSLNVRSQAAQVLYEASDPYSRKVIIDRGATNGVHVASPV
                     INETGVLGQVTRVYPLSSEVTLLTDKDAAIPVLNTRTQARGAAFGSAGATGGGMEMRF
                     MAGNADVQPGDVLTTSGVDGIYPPGLPVAKVASVDRKLDSGFARIVLTPNANPDGVRH
                     VMVIEPVGVQLPPRPEPAASAPEPKPGTAKKGSRAASRPGAAP"
     gene            complement(1355..2398)
                     /locus_tag="VFA35_01885"
     CDS             complement(1355..2398)
                     /locus_tag="VFA35_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZT54947.1"
                     /translation="MFGSFRRYFSTDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRHE
                     GGPNGKKTIQAVGAEAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPR
                     SVLRPSPRIIICVPCGSTQVERRAIRESALGAGASDVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSIRVGGDKFDESIINYIRRNYGMLIGEPTAEA
                     IKKSIGSAFPGSEVKEMEVKGRNLSEGVPRSFTISSNEILEALTDPLNQMVSAVKNAL
                     EQTPPELGADIAERGMMLTGGGALLRDLDRLLSEETGLPVLVAEDPLTCVVRGCGMAL
                     ERMERLGSIFTSE"
     gene            2628..2930
                     /gene="gatC"
                     /locus_tag="VFA35_01890"
     CDS             2628..2930
                     /gene="gatC"
                     /locus_tag="VFA35_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZT54948.1"
                     /translation="MALTPDDVSRIAQLARLELPPAEQAAMLTQLNGFFGIVEQMSAV
                     DTSGVEPLYTPLSAVEEVTLRLRDDAVTETDQRAANQRSAPAVEDGLFLVPKVIGG"
     gene            2927..>3800
                     /locus_tag="VFA35_01895"
     CDS             2927..>3800
                     /locus_tag="VFA35_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZT54949.1"
                     /translation="MKPIHEMGVAELGRAMADKQLSSVEATKHLLARVAAHEQLGAML
                     VADEAVALEHAQAADAKRAAGARGALLGVPLAHKDIFVTRELPTTAGSKMIEGYRSPF
                     DATVVARLAEAGVVTLGKLNCDEFAMGSSNENSAYRAVKNPWDASRVPGGSSGGSAAA
                     VAARLMPAATGTDTGGSIRQPASFCGITGIKPTYGVCSRYGMIAFASSLDQAGPLARS
                     AEDCALLLSAMSGFDPRDATSAERPPQDYAAALARQREGATAARPLQGLRIGLAKEFF
                     PAGLAGDVEKALRGA"
BASE COUNT          587 a         1316 c         1346 g          551 t
ORIGIN      
        1 agcaggcggc ggtggatggt cacggcgaag tagctgagca gcgtataggc gagcgcatgc
       61 tggccgagca cggcgccgct gtgcacgtcc atcagcaggc cgaaggcgaa ggcgacgccg
      121 acgccgaccc gacgcgactg gtgcacgttc cagaacacca gcaccagcgc gacgaagtcg
      181 ggcagcatcg gcacgcggcc gagcggcacg atgttcagcg cgaacgccac cagcagcgtg
      241 aacgcgacga acagcgggtt gaccggcagc agcagctggt cactggcacg cggcatgatc
      301 atggcgcggc cccgggtcgg gacgcggcgc gcgacccctt cttcgccgtg ccgggcttcg
      361 gctcgggcgc cgacgccgcc ggctcgggcc gcggcggcag ctgcacgccg accggctcga
      421 tcaccatcac gtggcgcacg ccgtcggggt tcgcgttcgg cgtcagcacg atgcgcgcga
      481 agcccgagtc gagcttgcgg tcgacgctcg cgaccttcgc caccggcagg cccggcgggt
      541 agatgccgtc gacgcccgag gtcgtcagca cgtcgcccgg ctgcacgtcg gcgttgccgg
      601 ccatgaagcg catctccatg ccgccgccgg tcgcgccggc gctgccgaac gccgcgccgc
      661 gcgcctgggt gcgggtgttg agcacgggga tcgcggcgtc cttgtcggtc agcagcgtga
      721 cttcgctgga cagcgggtac acgcgcgtga cctgcccgag cacgccggtc tcgttgatca
      781 ccggcgaggc cacgtgcacg ccgttggtcg cgccgcggtc gatgatgacc ttgcgcgagt
      841 acgggtccga ggcttcatac agcacctgcg ccgcctgcga cctgacgttc aggctcgggc
      901 gcagcgccag gagcccgcgc aggtgcgcgt tctcggccag caactgctcg acgcgcagcg
      961 cgcgctcgga ctgctgcgcg agcgcgcgtt tggcggccgc ctcgttcgcg agcgcgccct
     1021 gcaggccgag caggtactcg ccgccgccgc ggaccgcctg caccggcacg agcagcgcct
     1081 gttgcgggta gtacagcgcg gtcgcgaccg cggcgcgcag ctggcgcgtc agcgcgaagc
     1141 ggttgtcggc caccatcagg aacagcgcca gcgccgcgca caggatcagc ttggtcagcg
     1201 ccgacgggcc ctgccggaag aagggcggcg gggttcggtc gatggtaccg atggccatga
     1261 acgcaacgtc agcgtgaagg aatcgatgcg tcgaaacctg aagcgaaaaa accggccacg
     1321 gtgcgagcgt gccggcttgc gagggtcatg ccgctcactc ggaggtgaag atgctgccca
     1381 agcgctccat ccgctccagc gccatgccgc agccgcgcac cacgcaggtc agcgggtctt
     1441 cggcgaccag caccggcagg ccggtttctt cggacagcag gcggtccagg tcgcgcagca
     1501 gcgcgccgcc gccggtcagc atcatgccgc gctcggcgat gtcggcgccc agttcgggcg
     1561 gggtctgctc gagcgcgttc ttcacggccg agaccatctg gttcagcggg tcggtcagcg
     1621 cttccaggat ctcgttgctg ctgatcgtga agctgcgcgg cacgccttcg ctcaagttgc
     1681 ggcccttgac ttccatctcc ttgacctcgg agccggggaa cgcgctgccg atgctcttct
     1741 tgatcgcctc ggccgtcggc tcgccgatca gcatgccgta gttgcggcgg atgtagttga
     1801 tgatcgactc gtcgaacttg tcgccgccga cgcggatgct gcccttgtag accatgccgc
     1861 cgaggctgat cacgccgacc tcggtggtgc cgccgccgat gtcgacgacc atcgaaccgc
     1921 tggcctcgct gaccggcagc ccggcaccga tcgcggcggc catcggctcc tcgatcaggt
     1981 acacgtcgga ggcgccggcg ccgagcgccg actcgcggat cgcgcggcgc tcgacctgcg
     2041 tcgagccgca cggcacgcag atgatgatgc gcgggctcgg gcgcagcacc gagcgcgggt
     2101 gcaccatctt gatgaactgc ttgagcatct gctcggtgac ggtgaagtcg gcgatcacgc
     2161 cgtccttcat cgggcggatg gcctcgatgt tgccgggcac cttgccgagc atcgccttgg
     2221 cctcggcgcc gaccgcctgg atggttttct tgccgttggg cccgccttcg tggcggatgg
     2281 cgacgaccga cggctcgtcg agcacgatgc ccttgccacg cacgtagatc agcgtgttgg
     2341 cggtgccgag gtcgatggcc agatcagtcg agaagtagcg ccgaaaggat ccgaacatgg
     2401 tggtgggtgg tgagactcgt cacacggcct gcgggcaatg aagcaggcgc agcacggtgg
     2461 gacggattgt tgttgggggc ggcccgggca gtcggcaaat caccagcaaa cgacgatttg
     2521 cacaggccgc tggggcttgt ggataaccgg ggataatacc gcaagccctg cggctttcga
     2581 cgcgccgcgc cctgttctgg accgtcaaag aaccccccga tttccccatg gccctgaccc
     2641 ccgacgacgt gagccgcatc gcccagctgg cgcggctcga gctcccaccc gccgagcagg
     2701 ccgcgatgct cacccagctc aacggtttct tcggcatcgt cgagcagatg agcgcggtcg
     2761 acacgagcgg cgtcgagccg ctgtacacgc ccttgtcggc ggtggaggaa gtgacgctgc
     2821 ggctgcgcga cgacgcggtc accgagaccg accagcgcgc cgcgaaccag cgcagcgcac
     2881 cggccgtcga agacggcctt ttcctggtgc cgaaggtgat cggaggatga agccaattca
     2941 cgagatgggc gtggcggaac tgggccgcgc gatggcagac aagcagcttt ccagcgtcga
     3001 agcgacgaaa catctgctcg cacgggttgc cgcgcacgaa caactcggcg cgatgctggt
     3061 cgccgacgaa gcggtcgcgc tcgaacacgc gcaagcggcc gacgcgaagc gcgccgccgg
     3121 cgcacgcggc gcgctgctcg gcgtgccact cgcgcacaag gacatcttcg tcacgcgcga
     3181 gctgccgacg accgccggct cgaagatgat cgagggctac cgcagcccgt tcgacgcgac
     3241 cgtggtggcg cgcctggcgg aggccggtgt cgtcacgctc ggcaagctca attgcgacga
     3301 gttcgcgatg ggttccagca acgagaactc ggcgtatcgc gcggtcaaga acccgtggga
     3361 cgcatcgcgc gtgccgggcg gctcgtcggg cggctcggcc gccgcagtag cggcgcgcct
     3421 gatgcccgcc gcgaccggca ccgacaccgg cggctcgatc cgccagccgg ccagcttttg
     3481 cggcatcacc ggcatcaagc cgacctacgg ggtgtgctcg cgctacggca tgatcgcctt
     3541 cgcctcgagc ctcgaccagg ccgggcccct ggcgcgcagc gccgaggact gcgccctgct
     3601 gctttcggca atgagcgggt tcgatccgcg cgacgcgacc agcgccgagc ggccgccgca
     3661 ggactatgcg gccgcgctcg ccaggcagcg cgagggcgcc actgccgccc ggccgctgca
     3721 gggcctgcgc atcggcctgg cgaaggaatt cttccccgcc gggctggccg gcgacgtcga
     3781 gaaggccttg cgcggcgcgc
//
LOCUS       DASRDG010000112         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_943362, whole genome shotgun sequence.
ACCESSION   DASRDG010000112 DASRDG010000000
VERSION     DASRDG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943362"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2771)
                     /locus_tag="VFA35_01900"
     CDS             complement(<1..>2771)
                     /locus_tag="VFA35_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54950.1"
                     /translation="ARYKNRVYALDHPTLGESPITNALALVKALPAGARLHLVTHSRG
                     GLVAEVLARACGGGTLGADELALFGDARYAVHRADLQALVTLAQKKGLKVERVVRVAC
                     PARGTLLASKRLDAYLSVLQWCLQLAGLTVSAELVDFLHEVALRRADPAELPGLEAMM
                     PDSKVVEWLNTAGEAVPGELRVIAGDMQGDSIASWVKTLLSDAFYWTDNDLVVQTRSM
                     YGGAPRQSAPNAPPAASFLLDRGAKVSHFNYFANDRTVGAIAAALHDAEPADFDAIGP
                     LSWAGKDASGSRAAKAVARSRGAPGTTAAERPAVIVLPGILGSNLKLDGKRIWLGFRF
                     INGLKKLAWDPATASRVEPDGPIGSSYDDLIEHLADTHEVIPFAYDWRRPIEDEARRL
                     AVEIDARLAERSASQQPVRIVAHSMGGLLARTLRLEKPDTWKRMMARDGARLLMLGTP
                     NGGSWAPMQTLSGDDTFGNALVAIGSLFDNSGMRKLMAAMPGFIQLQAGLLDPALGLD
                     QEATWTRLAHDDIKALLARSAWHDEGAQRTIYEWFAPPQAVLDQAVALRRRLDAQVPE
                     LGSDTQKMLLVVGHADFTPDGFTIGVDGLEYDNVPDDGDGRVPLASARLPGVRTWKLD
                     AEHGKLPDVSSAFGAYVELLTTGQTRQLGALEPATRGAPGAAPAHVRSRPSRGLNGAR
                     PPSSESDVFTSGPQGLAAGRSGRGASAALSVTVLNGDLKFVRHPLLIGHYRPLTLNGA
                     EAVVDRLVDRSMSRSFEAGLYPDEPGSHQIFGNLRRNPDNPFEMARPAAVVVAGLGEE
                     GKLKAGDLVFTVRQAVLAYAQRLAEREGGAPAQFELAATLIGSGGTGITPGSSAQLIA
                     QGALDANQKLQENGWPQLAQLTLVELYLERAADAWRALQLQEQAAPTRLRLVGKVGFG
                     PGAMRRSLD"
BASE COUNT          341 a         1012 c         1032 g          386 t
ORIGIN      
        1 gtcgagcgag cggcgcatcg cacccgggcc gaagccgacc ttgccgacca gccgcagccg
       61 cgtcggcgcg gcctgctcct gcagttgcag cgcgcgccag gcgtcggcgg cgcgctcgag
      121 gtagagctcg accagcgtca gctgcgcgag ctgcggccag ccgttctcct gcagcttctg
      181 gttcgcgtcg agcgcaccct gcgcgatcag ctgggccgag ctgcccggcg tgatgccggt
      241 gccgccgctg ccgatcagcg tggccgccag ctcgaactgc gccggcgcgc cgccttcgcg
      301 ctccgccagc cgctgcgcat acgccagcac cgcctggcgc accgtgaaga ccaggtcgcc
      361 ggccttgagc ttgccttcct cgccgagccc ggcgacgacg accgccgccg gccgcgccat
      421 ctcgaacggg ttgtcggggt tgcggcgcag gttgccgaag atctggtgcg agccgggctc
      481 gtcggggtac aggccggcct cgaacgagcg gctcatgctg cggtcgacga gccggtcgac
      541 cacggcctcg gcgccgttga gcgtcagcgg gcggtagtgg ccgatcagca gcgggtggcg
      601 cacgaacttc aggtcgccgt tcagcaccgt cacgctcagc gcggccgacg cgccgcggcc
      661 gctgcggccg gcggccaggc cctgcggccc gctcgtgaac acgtcggact cgctcgacgg
      721 cggccgcgcg ccgttcaggc cgcgcgacgg ccggctgcgc acgtgcgcgg gcgccgcgcc
      781 gggcgcgccg cgcgtcgcgg gctcgagcgc gccgagctgg cgcgtctgcc ccgtcgtcag
      841 cagctcgacg taggcgccga acgccgagct cacatcgggc agcttgccgt gctcggcgtc
      901 gagcttccag gtgcgcacgc ccggcaggcg cgcgctggcc agcggcacgc ggccgtcgcc
      961 gtcgtcgggc acgttgtcgt attcgaggcc gtcgacgccg atcgtgaagc cgtcgggcgt
     1021 gaagtcggca tggccgacca cgagcagcat cttctgcgtg tcgctgccga gttcgggcac
     1081 ctgcgcgtcg agccggcgcc gcagcgccac cgcctggtcg agcaccgcct gcggcggcgc
     1141 gaaccattcg tagatcgtcc gctgcgcgcc ttcgtcgtgc caggcgctgc gcgcgagcag
     1201 cgctttgatg tcgtcgtgcg cgagccgggt ccaggtggct tcctggtcga gcccgagggc
     1261 ggggtcgagc aggccggcct ggagctggat gaagccgggc atcgcggcca tcagcttgcg
     1321 catgccgctg ttgtcgaaca gcgagccgat cgccaccagc gcgttgccga aggtgtcgtc
     1381 gcccgagagc gtctgcatcg gcgcccacga gccgccgttg ggcgtgccca gcatcagcag
     1441 gcgcgccccg tcgcgcgcca tcatgcgctt ccaggtgtcg ggcttctcga ggcgcagcgt
     1501 gcgcgcgagc agcccgccca tcgagtgcgc gacgatgcgc accggctgct ggctggcgct
     1561 gcgctcggcg agccgggcgt cgatctcgac ggccaggcgc cgcgcttcgt cctcgatcgg
     1621 gcgccgccag tcgtaggcga acgggatcac ctcgtgggtg tcggccaggt gctcgatcag
     1681 gtcgtcgtag ctgctgccga tcgggccgtc gggctcgacg cgcgacgcgg tcgccgggtc
     1741 ccaggcgagc ttcttcaggc cgttgatgaa acgaaagccg agccagatgc gcttgccgtc
     1801 gagcttgagg ttcgagccga ggatgccggg cagcacgatg acggccgggc gctcggccgc
     1861 ggtcgtgccc ggcgcgccgc gcgagcgcgc caccgccttc gcggcgcgcg aaccgctcgc
     1921 gtccttgccg gcccacgaga gcgggccgat ggcgtcgaag tcggccggtt cggcgtcgtg
     1981 cagcgcggcg gcgatcgcgc cgacggtgcg gtcgttggcg aagtagttga agtgcgagac
     2041 cttggcgccg cgatcgagca ggaagctcgc cgccggcggc gcgttcggcg cgctctggcg
     2101 cggcgcgcca ccgtacatcg agcgcgtctg cacgacgagg tcgttgtcgg tccagtagaa
     2161 cgcgtcggag agcagggtct tgacccacga cgcgatcgaa tcgccctgca tgtcgccggc
     2221 gatcacgcgc agctcgccgg ggaccgcctc gcccgccgtg ttgagccact cgacgacctt
     2281 gctgtcgggc atcatcgcct cgagcccggg cagttcggcc gggtcggcgc ggcgcagcgc
     2341 gacctcgtgc aggaagtcga ccagttccgc gctcaccgtc agccccgcga gctgcaggca
     2401 ccactgcagc accgacaggt aggcgtcgag ccgcttcgac gccagcagcg tgccgcgcgc
     2461 cgggcaggcg acgcgcacga cgcgctcgac cttcaggccc ttcttctgcg ccagcgtcac
     2521 gagcgcctgc aggtcggcgc ggtgcaccgc gtagcgcgcg tcgccgaaca gcgcgagctc
     2581 gtcggcaccc agcgtgccgc cgccgcaggc ccgcgcgagc acctcggcga cgaggccgcc
     2641 gcgcgagtgc gtgacgaggt gcagccgcgc gcccgccggc agcgccttga cgagcgcgag
     2701 cgcgttggtg atcgggctct cgcccagcgt cgggtggtcg agcgcgtaga cccggttctt
     2761 gtagcgcgcg a
//
LOCUS       DASRDG010000113         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_375029, whole genome shotgun sequence.
ACCESSION   DASRDG010000113 DASRDG010000000
VERSION     DASRDG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375029"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VFA35_01905"
     CDS             complement(<1..1356)
                     /locus_tag="VFA35_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HZT54951.1"
                     /translation="MNTSTTAVSRLPSAPAAAATGARWAWLPPIGSARYYLMLGLLGA
                     LVLGPLGGVTASYMNFSIGFFVGGQVLAGILGSTVTLGYGPEGKHGANYMQTAAASVA
                     GMAGMGVLLQAMVWMGLPQPPLWQLMLFMGAIGMFGAGVGMLYTPILVDRLQLTFPSG
                     LAVANILRALTDPVLLRQSIARLGTGMGLGMFGGIGAAKLGFLGVIELSTSTLGAGML
                     VGARIGIAALVAGVTGHLLIPYFISIGWLHAGEPFRKITFLIGLGLIMGAAILDIGQL
                     LVEAARRWRAVAQLAAPAAAPVDEDWKRTSTGRLLAWVAFWGAAVVWVGAAVLHQPVF
                     FMAVAIALVFVFAMVNGISLGISDSNPISSAFVTAVVLMAALGLKDPIVGLMAGSVLL
                     VACSVAGDMQQDRSTGWRLGTPRTIQFRFQVLGLVVGAVLTVGFAKLFMSAYPVLNLD
                     QT"
     gene            1497..2273
                     /locus_tag="VFA35_01910"
     CDS             1497..2273
                     /locus_tag="VFA35_01910"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT54952.1"
                     /translation="MKKSLLVLAVVVALLPLAAPAAEATLKLRVGVEGAYPPFSSVAA
                     DGKIVGFDIDIANALCAQMKAQCTLVQQDFDAMIPALQAKKIDVVIASMSATPERLKV
                     VDFSDKYYNTPNRLIAKAGTTIDGTPASFKGKKIGVQRSTINDRFATAKFAGATIVRY
                     TKQDEVFLDLAAGRVDATLVDAVAGDVGFLKLPAGKGFAFTGPVYDDPAYFGTGVGVA
                     LRKGEAPLREQLNAAIKAIRANGVYKTLQGKYFDFDVYGK"
     gene            2283..2969
                     /locus_tag="VFA35_01915"
     CDS             2283..2969
                     /locus_tag="VFA35_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830462.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZT54953.1"
                     /translation="MLQGYGASLLQGAALTLAVALGALLLALALGLAGAAAKLSNSGF
                     WRGAAGVYTSVIRGVPDLVLMMLIFYGGQIALNAALGALGREGVDIDPFVAGVATLGV
                     IFGAYFTETFRGAILAVPPGQIEAGRAFGMSAWGVFRRITAPQMLRFAIPSFTNNWLV
                     LVKSTALVSLIGLNDMLQKAQQAAGATRQPFTFYLAVGALYLAVTSVSVLALRALEHR
                     VSRGAKVAVL"
BASE COUNT          472 a         1052 c         1079 g          411 t
ORIGIN      
        1 ggtctggtcg aggttgagca cggggtacgc gctcatgaag agcttggcga agcccaccgt
       61 cagcacggcg cccaccacga ggcccagcac ctggaagcgg aactggatcg tgcgcggcgt
      121 gcccagccgc cagccggtcg agcggtcctg ctgcatgtcg ccggcgacgc tgcaggccac
      181 cagcagcacg ctgccggcca tcagcccgac gatcgggtcc ttcaggccga gcgcggccat
      241 cagcaccacc gcggtgacga aggccgacga gatcgggttg ctgtcgctga tgcccagcga
      301 gatgccgttg accatcgcga acacgaacac cagggcgatc gcgaccgcca tgaagaacac
      361 cggctggtgc agcacggcgg cgccgaccca gaccaccgcc gcgccccaga acgcgaccca
      421 ggcgagcagc cggccggtgc tggtgcgctt ccagtcttcg tcgaccggcg cggcggcggg
      481 cgccgcgagc tgcgcaaccg cgcgccaacg ccgggcggcc tcgaccagga gctggccgat
      541 gtcgaggatc gcggcgccca tgatcagccc gaggccgatc aggaaggtga tcttgcgaaa
      601 cggctcgccc gcgtgcagcc agccgatcga gatgaagtag gggatcagca ggtggccggt
      661 gacgccggcg accagcgccg cgatgccgat gcgcgcgccg accagcatgc cggcgccgag
      721 cgtcgaggtc gagagctcga tcacgcccag aaagccgagc ttggccgcgc cgatgccgcc
      781 gaacatgccg agccccatgc cggtgccgag ccgcgcgatc gactggcgca gcagcaccgg
      841 gtcggtcagc gcccgcaaga tgttcgcgac cgccaggccc gacgggaagg tgagctgcag
      901 ccggtcgacc aggatcggcg tgtacagcat gccgacgccg gcgccgaaca tgccgatcgc
      961 gcccatgaac agcatcagct gccacagcgg cggctgcggc agccccatcc agaccatcgc
     1021 ctggagcagc acgcccatgc ccgccatgcc ggcgaccgag gccgccgcgg tctgcatgta
     1081 gttcgcgccg tgcttgcctt cggggccgta gcccagcgtc acggtcgagc cgaggatgcc
     1141 ggccagcacc tgcccgccga cgaagaagcc gatcgagaag ttcatgtacg acgcggtcac
     1201 gccgccgagc ggccccagca ccagcgcgcc gagcaggccc agcatcaggt agtagcgcgc
     1261 gctgccgatc ggcggcagcc aggcccagcg tgcgccggtc gccgcggcgg cgggggccga
     1321 ggggagtcga gaaacggcgg tggtcgaagt gttcatgcgg cgagggcgat gcgcgcgcgg
     1381 cccggatcgc gggccggcgc gccatcgcgg gaatgggcgg tggccggatt gtggcgcgtc
     1441 cgcaggccgc tacagtgcgg catgcggcgc catctgcccg cctgccggag tcgttcatga
     1501 agaagtcgct gcttgtcctt gccgtcgtcg ttgcgctgtt gccgctggcc gcaccggccg
     1561 ccgaagcgac gctgaagctg cgcgtcggcg tcgagggcgc gtacccgccg ttcagctcgg
     1621 tcgccgccga cgggaagatc gtcggtttcg acatcgacat cgcgaacgcg ctgtgcgcgc
     1681 agatgaaggc gcagtgcacg ctggtgcagc aggacttcga cgcgatgatc ccggcgctgc
     1741 aggcgaagaa gatcgacgtc gtcatcgcgt cgatgtcggc cacgcccgag cggctcaagg
     1801 tggtcgactt ctccgacaag tactacaaca cgccgaaccg gctgatcgcc aaggccggca
     1861 ccacgatcga cggcacgccg gcgagcttca agggcaagaa gatcggcgtg cagcgcagca
     1921 cgatcaacga ccgcttcgcg accgccaagt tcgccggcgc gaccatcgtg cgctacacca
     1981 agcaggacga ggtcttcctc gacctcgccg ccggccgcgt cgatgcgacg ctggtcgacg
     2041 cggtcgccgg cgacgtcggc ttcctgaagc tgccggccgg caagggcttt gccttcaccg
     2101 ggccggtcta cgacgacccg gcctacttcg gcaccggcgt cggtgtcgcg ttgcgcaagg
     2161 gcgaggcgcc gctgcgcgag cagctcaacg ccgcgatcaa ggcgatccgc gccaacggcg
     2221 tgtacaagac gctgcagggc aagtacttcg acttcgacgt gtacggcaag taggcccgcg
     2281 cgatgctcca gggctacggc gcgagcctgc tgcagggtgc ggcgctgacg ctggcggtcg
     2341 cgctcggcgc gctgctgctc gcgctcgcgc tcggcctggc cggtgcggcg gcgaagctgt
     2401 cgaactcggg cttctggcgc ggtgccgcgg gcgtctacac cagcgtgatc cgcggcgtgc
     2461 ccgacctggt gctgatgatg ctgatcttct acggcgggca gatcgcgctg aacgcggcgc
     2521 tcggcgcgct cggacgcgag ggcgtcgaca tcgacccgtt cgtcgccggc gtcgccacgc
     2581 tcggcgtcat cttcggcgcg tacttcaccg agaccttccg cggcgcgatc ctggcggtgc
     2641 cgccggggca gatcgaggcc gggcgcgcgt tcggcatgtc ggcgtggggt gtgttccggc
     2701 gcatcaccgc gccgcagatg ctgcgcttcg cgatcccctc gttcacgaac aactggctgg
     2761 tgctggtcaa gtcgaccgcg ctggtctcgc tgatcggcct gaacgacatg ctgcagaagg
     2821 cgcagcaggc ggccggcgcg acgcgccagc cgttcacgtt ctaccttgcg gtcggcgcgc
     2881 tgtacctcgc ggtcacgagc gtttcggtgc tcgcgctgcg cgcgctcgaa caccgcgtgt
     2941 cgcgcggcgc gaaggtggcg gtgctgtgaa cgccgcggcc atcgccgaga tgctgccgac
     3001 ctactgggaa ggcc
//
LOCUS       DASRDG010000114         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_252673, whole genome shotgun sequence.
ACCESSION   DASRDG010000114 DASRDG010000000
VERSION     DASRDG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252673"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1189)
                     /locus_tag="VFA35_01920"
     CDS             complement(80..1189)
                     /locus_tag="VFA35_01920"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HZT54954.1"
                     /translation="MDFRGRVLLAPLTKGGNLPFRRLCTAFGCATTMSEMAYAFQVVR
                     RRPTELALLRKHADEPCFGVQLAASKPADAIAAGLAAVERGAAFVDLNCGCPIHDVVK
                     RGMGAVLLQRPAHLGRLVEAMVKALPVPVTVKIRLGWSEADKNASEVAQAVQAAGASA
                     LSVHGRTREQRYTKAADWDAIARLVGELTIPVIGNGDILTWYEAQDLQRKSGCASVMV
                     GRGALIKPWLFRELAEGKEFEPGAEQRVAIYMQFVRCLKEHFRDDEKGRKRAMFFLPW
                     HLGFFCRYRPLPEARFREQSRAHPLLQTRMPDRDEVSLLERLLRDPREQVHALLAAAL
                     WDAADDADAAARFLSIAAANPPGSGEAAEVAVAHG"
     gene            1279..1815
                     /locus_tag="VFA35_01925"
     CDS             1279..1815
                     /locus_tag="VFA35_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54955.1"
                     /translation="MHPATIVGCGIALAALSLVFAAPPAASTAASPTASAAPSTAGPP
                     PQAPLVQDPAALGHLALIVEGNVGLLAITHAVAKTDPWGGVPTGLKSNFELRLLGDDG
                     VELLKVPVDLSPFETDPARIGGPVEVTGCQVRSSAIALIVNVPRLAGVRSYQFTRRGA
                     PIGSTGAQRVAELLEERR"
     gene            1812..>2124
                     /locus_tag="VFA35_01930"
     CDS             1812..>2124
                     /locus_tag="VFA35_01930"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HZT54956.1"
                     /translation="MIRSIAGAALLAAAVAAQAPIVNTVVNNGSTASHYDLVFLGDGY
                     QAAQRTQFDQDVLTCVTSMFQHVPYSTFASYFNVHTVFRASVDAGASQPDVQPPIVRN
                     TA"
BASE COUNT          303 a          793 c          727 g          301 t
ORIGIN      
        1 gtcggtggcg tagaagtgga tgcgcacgcc ttcgtgcttc gtcgcgcgcc aggggatcgc
       61 ggcaaggtcc acgccgggcc tacccgtgcg cgacggcgac ctcggccgcc tcgcccgaac
      121 cgggcgggtt ggccgccgcg atcgagagaa accgcgcggc cgcgtcggcg tcgtcggcgg
      181 cgtcccacag cgccgcggcc agcagcgcgt gcacctgctc gcgcggatcg cgcaggaggc
      241 gctcgagcag cgacacctcg tcgcggtccg gcatgcgcgt ctgcagcagc ggatgcgcgc
      301 ggctctgctc gcggaaccgc gcttcgggca gcggccggta acggcagaag aagccgaggt
      361 gccagggcag gaagaacatc gcgcgcttgc gccccttctc gtcgtcgcgg aagtgctcct
      421 tgagacagcg cacgaactgc atgtagatcg cgacgcgttg ctcggcgccc ggctcgaact
      481 ccttcccttc cgcgagctcg cggaacaacc acggcttgat cagcgcgccg cgccccacca
      541 tcaccgacgc gcagccggac ttgcgctgca ggtcctgcgc ctcgtaccac gtgaggatgt
      601 cgccgttgcc gatcaccggg atcgtcagct cgcccacgag ccgcgcaatc gcgtcccagt
      661 cggcggcctt cgtgtagcgc tgctcgcgcg tgcggccgtg cacggacagc gccgacgcgc
      721 ccgccgcctg cacggcctgc gcgacctcgc tcgcgttctt gtcggcctcg ctccaaccga
      781 gccggatctt gacggtgacc ggcaccggca gcgccttcac catcgcctcg acgagccggc
      841 cgaggtgcgc aggccgctgc agcagcacgg cgcccatgcc gcgcttgacc acgtcgtgga
      901 tcgggcagcc gcagttgagg tcgacgaacg cggcgccgcg ctcgaccgcg gcgagcccgg
      961 cggcgatcgc gtccgccggc ttcgacgcgg cgagctgcac gccgaagcac ggttcgtcag
     1021 cgtgcttgcg cagcagcgcg agttcggtcg gccgccggcg cacgacctgg aacgcgtacg
     1081 ccatctccga catcgtcgtc gcgcagccga aggccgtgca gaggcgccgg aacggcaggt
     1141 tgccgccctt cgtcaggggc gcgagcagca cacggccacg gaagtccatg ggcggagatc
     1201 ctgtcgtcgg tgcaacagct tgtccacggc gccggtccga cggctaggat tctccgcgcc
     1261 ccaaccgtct cgtctgccat gcacccagca accatcgtcg gctgcggcat cgcgctcgca
     1321 gcactgagtc tcgtattcgc tgcgccgccc gccgcgtcga ccgctgcatc gcccacggca
     1381 tcggccgcgc catcgaccgc cggaccaccg ccgcaggcgc ccctggtcca ggaccccgct
     1441 gcgctcggcc acctcgcgct gatcgtcgaa ggcaacgtag ggctgctcgc catcacccat
     1501 gcagtcgcca agaccgatcc gtggggcggc gtcccgaccg gcctgaagag caacttcgag
     1561 ctgcggctgc tcggcgacga cggcgtcgag ctgctgaagg tgccggtcga tttgtcgccg
     1621 ttcgagaccg atccggcgcg catcggcggg cccgtggagg tcaccggttg ccaggtgcgg
     1681 agcagcgcga tcgcgctgat cgtcaacgtg ccgcggctcg ctggcgtgcg cagctaccag
     1741 ttcacgcggc gcggcgcgcc gatcggcagc accggtgcgc agcgggtggc cgagctgctg
     1801 gaggagcgcc gatgatccgc tccatcgccg gcgccgcgct gctcgcggcc gccgtcgccg
     1861 cgcaggcgcc gatcgtgaac accgtcgtca acaatggttc gacggcgagc cactacgacc
     1921 tggtgttcct cggcgacggc taccaggccg cacagcggac gcagttcgac caggacgtgc
     1981 tgacctgcgt gacgtcgatg ttccagcacg tgccgtactc gacgttcgcg agctacttca
     2041 acgtgcacac cgtgttccgc gccagcgtgg acgccggcgc gtcgcagccc gacgtgcagc
     2101 cgccgatcgt gcgcaacacc gcct
//
LOCUS       DASRDG010000115         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1400875, whole genome shotgun sequence.
ACCESSION   DASRDG010000115 DASRDG010000000
VERSION     DASRDG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400875"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2172)
                     /locus_tag="VFA35_01935"
     CDS             complement(<1..>2172)
                     /locus_tag="VFA35_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HZT54957.1"
                     /translation="SPTVPPGPQHPIDAFVDRELQQHGLAPAPAADRRTLARRLWFDL
                     TGLPPTPEELDAFVADPSPTAYEDLVARLLDSPRYGERWARHWMDAVHFAETHGHDQD
                     RIREHAWPYRDYLIASFNADKPYARFVEEQVAGDALFPDEPQACVALGMLAAGPWDES
                     SLRDIREDTLDREIGRYLDRDDVVTTVMQTFTSTTVQCARCHDHKFDPISQRDYYALQ
                     AVFAGIGRGNLRYDGDPATGRRRVELLARRAAAEQGDPATLAGAEAQRVAAEWERQQT
                     AANAWQVLQPDVFVSGAGATLTRQPDGSFLASGPHGPTDTYTFTAHAPLPRLTAVRLE
                     VLPDDSLSMRGPGRESNGNLHLSEFQLLSFAPGAAAPARIELRRPSADFEQQDWTIAH
                     ALDGKDETAWGIFPRVGQPHEAVFETAAAVDLSAATQLTFVLRQLHGGHCIGRLRLSV
                     TDAAAPASALAQEVRDVLATPAERRSDAQRALLARTALLQAIDRQLDALPPQALVYCG
                     CRDFEPDGGHKPVREPRPVHLLRRGEITKPEEAAAPGALGCVTALPARFALRTPLDEG
                     ERRAALAHWLTDPRNPLTWRSIVNRVWQRHFGSGLVATPNDFGRMGGTPSHPELLDWL
                     AVWFRGDAHGSLKQLHRLIVTSAAYRRAAAYPDAEAFARAAAVDADDRLLWRRLPQRL
                     DAEQVRDAVLQISGRLDLHMGGPSDRMFDLQPGIHVTPRVDY"
BASE COUNT          284 a          769 c          819 g          300 t
ORIGIN      
        1 gtaatcgacc cgcggcgtga cgtggatgcc gggctgcagg tcgaacatac ggtcgctcgg
       61 gccgcccata tggagatcga gccgccccga gatctgcagc acggcgtcgc gcacctgctc
      121 ggcgtcgagc cgctgcggca gacggcgcca gagcaggcga tcgtcggcgt cgacggccgc
      181 ggccctcgcg aaggcctccg cgtcgggata cgccgcggcg cggcgatagg ccgcgctcgt
      241 gacgatgagc cggtgcagct gtttgaggga gccgtgcgcg tcgccgcgga accacactgc
      301 gagccagtcc agcagttcgg gatgcgacgg cgtgccgccc atgcggccga agtcgttggg
      361 cgtcgccacc agtccgctgc cgaagtgccg ctgccagacg cggttcacga tcgaccgcca
      421 ggtcagcgga ttgcgcggat cggtgagcca gtgggccagc gccgcgcggc gctcgccttc
      481 gtcgagcggc gttcgcagag cgaaccgcgc cggcaatgcc gtgacgcagc cgagcgcgcc
      541 gggcgccgcc gcctcctccg gcttcgtgat ctcgccgcgg cgcagcagat gcacgggccg
      601 cggctcgcgc acgggcttgt ggccgccgtc cggttcgaaa tcgcggcagc cgcagtagac
      661 gagcgcctgc ggcggcagtg cgtcgagctg ccggtcgatc gcctgcaaca gcgcggtgcg
      721 ggcgagcagc gcgcgctgcg catccgaacg ccgttccgcc ggcgtcgcga gcacgtcgcg
      781 cacttcctgc gcgagcgccg atgcgggcgc cgcggcgtcc gtcaccgaca ggcgcaaccg
      841 gccgatgcaa tgaccgccgt gcagctggcg cagcacgaag gtcagctgtg ttgctgccga
      901 caggtcgacg gctgccgccg tctcgaacac cgcctcgtgg ggctggccga cgcgcgggaa
      961 gatgccccag gccgtctcgt ccttgccgtc gagggcgtgc gcgatcgtcc agtcctgctg
     1021 ctcgaagtcc gcggatggac ggcgcaattc gatgcgcgca ggcgccgcgg ctcccggtgc
     1081 gaacgagagc agctggaact cggacaggtg caggttgccg ttgctctcgc ggccgggccc
     1141 gcgcatcgac agcgagtcgt cgggcagcac ctcgagccgc acggcggtga ggcgggggag
     1201 cggcgcgtgg gcggtgaacg tgtaggtgtc cgtcggcccg tgcgggccgc tcgcgaggaa
     1261 cgaaccgtcc ggctggcgag tcagcgtggc gccggccccc gacacgaaca cgtcgggctg
     1321 caggacctgc cacgcgttcg ccgcggtctg ctgccgttcc cactcggcgg cgacgcgctg
     1381 cgcctcggcg ccggccagcg tcgccggatc gccctgctcc gccgccgccc ggcgcgccag
     1441 cagctcgacg cggcggcggc cggtcgccgg gtctccgtca tagcgcaggt tgccgcggcc
     1501 gatgcccgcg aacacggcct gcagggcgta gtagtcgcgc tggctgatcg ggtcgaactt
     1561 gtggtcatga cagcgcgcgc actgcacggt cgtcgacgtg aaggtctgca tcacggtcgt
     1621 gacgacgtcg tcgcggtcga ggtagcggcc gatctcgcgg tcgagcgtgt cctcgcggat
     1681 gtcgcgcagc gagctctcgt cccacggccc ggcggcgagc atgcccagcg ccacgcacgc
     1741 ctgcggctcg tcggggaaca gcgcgtcgcc ggcgacctgc tcctcgacga agcgcgcgta
     1801 cggcttgtcg gcgttgaagc tcgcgatcag gtaatcgcgg tacggccacg catgttcgcg
     1861 gatgcggtcc tggtcgtggc cgtgcgtctc ggcgaagtgc acggcgtcca tccagtgccg
     1921 cgcccaccgt tcgccgtagc gcgggctgtc gagcagccgg gcgaccagat cctcgtacgc
     1981 cgtcggcgac gggtcggcca cgaacgcgtc cagctcctcg ggcgtcggcg gcagaccagt
     2041 gaggtcgaac cagagccgcc gcgccagcgt ccgccgatcg gcggcgggcg ccggcgcgag
     2101 tccgtgctgc tgcagctcgc ggtcgacgaa ggcgtcgatc ggatgctgtg gccccggtgg
     2161 cacggtcggc ga
//
LOCUS       DASRDG010000116         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1527184, whole genome shotgun sequence.
ACCESSION   DASRDG010000116 DASRDG010000000
VERSION     DASRDG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527184"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..385)
                     /locus_tag="VFA35_01940"
     CDS             complement(104..385)
                     /locus_tag="VFA35_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZT54958.1"
                     /translation="MLLLPATVTAKEANDTQRLLTQALKGEAQADVVVDASNLQHFDS
                     AALAVLLECERAAEGFGKSFALRNAPPKLAALARLYGVDALLMPEPTAG"
     gene            complement(395..1021)
                     /locus_tag="VFA35_01945"
     CDS             complement(395..1021)
                     /locus_tag="VFA35_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT54959.1"
                     /translation="MLLKRMIPWLAAGLLSVGCVVAQAQAGNAPDALIKEVSTDVLET
                     LKADKSYKQGDLQKIITLVDTKVMPYVDFQRMTASAVGRYWRQATPEQQKSLQDQFKL
                     LLVRTYSGALAQVSDQTVNLKPMRGAATDAEVVVKTEIKGRGDPIQLDYRLEQTPGGW
                     KIYDVNVLGVWLVENYRNTFSQEIGAGGIDGLISKMTARNKAAGAAKN"
     gene            complement(1126..1959)
                     /locus_tag="VFA35_01950"
     CDS             complement(1126..1959)
                     /locus_tag="VFA35_01950"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZT54960.1"
                     /translation="MTRSPATHRPRARTLPLLLGALLVLGGCATTQALVNDARGGPGA
                     RLDPWENWNRKVFAFNEGLDEKILKPIATGYAKVVPQFVRRSIDNFFANAADAWSAVN
                     NVLQGKPEPAFTDVVRFTTNTVFGIFGILDIASEFGLEHHYEDFGQTLGRWGFGPGAY
                     IVWPLIGPSSVRETIALPLDRAASPALYINDGRYQFGVVGLQIINTRANLLGASQVID
                     DIALDKYSFIRDAYLQRRRSLVFDGDAPEAPEAPDTPPPAATPASGAAPAGSASAPGK
                     P"
     gene            complement(1956..>2284)
                     /locus_tag="VFA35_01955"
     CDS             complement(1956..>2284)
                     /locus_tag="VFA35_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HZT54961.1"
                     /translation="SAGVTVGRVASISLDAKRYQGAVRIEIDRAVQFPSDSSAHILTS
                     GLLGDQYVGLEAGASEKNFAAGDTIKQTQSAIVLESLISQFLFSKAADAGSTSTPAPA
                     ANGGKK"
BASE COUNT          333 a          756 c          787 g          408 t
ORIGIN      
        1 tcggcctggt gggcgctgac cgggctggtg gtcacgagct tcgcgttcct cggcgtgaac
       61 atgttcctgt ccggcctgca ctcctacggc acgctgtagc tttctagcca gcggtcggct
      121 cgggcatcag cagcgcgtcg acaccgtaga gccgcgccag cgccgccagc ttcggcggcg
      181 cattgcgcag cgcgaacgac ttgccgaagc cctcggctgc gcgctcgcat tcgagcaaaa
      241 cggcgagcgc ggccgagtcg aagtgctgca ggttcgacgc gtcgaccacc acgtcagcct
      301 gcgcctcgcc cttcagcgcc tgcgtcagca ggcgctgcgt gtcgttcgct tccttggcgg
      361 tgaccgtggc gggcagcaac agcatcggcg ccggtcagtt cttggccgcg ccggccgcct
      421 tgttgcgcgc cgtcatcttc gagatcaggc cgtcgatgcc gccggcgccg atctcctgcg
      481 agaaggtgtt gcggtagttc tcgaccagcc acacaccgag cacgttgacg tcgtagatct
      541 tccagccgcc cggcgtctgc tcgagccggt agtcgagttg gatcgggtcg ccgcgcccct
      601 tgatctcggt cttgacgacg acttcggcat cggtcgcggc gccgcgcatc ggcttcaggt
      661 tcaccgtctg gtcggacacc tgcgcgagcg cgccggagta ggtgcgcacg agaagcagct
      721 tgaactgatc ctgcaagctc ttttgctgct cgggcgtggc ctggcgccaa tagcggccga
      781 cggccgacgc ggtcatgcgc tggaagtcga catacggcat gaccttggtg tcgaccagcg
      841 tgatgatctt ctgcagatcg ccctgcttgt acgacttgtc ggccttgagc gtctcgagca
      901 cgtcggtcga gacctccttg atcaatgcat cgggggcgtt gccggcctgg gcctgggcca
      961 ccacacaacc gacactgagc aggccggcag ccagccacgg aatcattcgt ttcaacaaca
     1021 tcttgtttcc ttcttcgttg cgacaggacg gccccgtcgg agtccaggcg aaccggttgg
     1081 ttcagcgcgg cctgcatcct tcaacgcgcg aacccggtgc gcggttcacg gcttgcccgg
     1141 cgccgacgcc gaacctgccg gtgcggcgcc cgatgccggc gtggcggccg gcggtggcgt
     1201 atctggcgct tcaggggctt cgggcgcgtc gccgtcgaag accaggctgc gccggcgttg
     1261 caggtaggcg tcgcggatga agctgtactt gtcgagcgcg atgtcgtcga tcacctggct
     1321 cgcgccgagc agattggcgc gggtgttgat gatctgcagc ccgaccacgc cgaactggta
     1381 gcggccgtcg ttgatgtaga gcgccggcga ggcggcgcga tcgagcggca gggcgatcgt
     1441 ctcgcgcacc gacgacggcc cgatcagcgg ccacacgatg taggcgccgg gcccgaagcc
     1501 ccagcggccg agcgtctggc cgaagtcttc atagtgatgc tcgaggccga actcgctcgc
     1561 gatgtcgagg atgccgaaga tgccgaacac ggtgttggtc gtgaagcgca cgacatccgt
     1621 gaacgccggc tcgggcttgc cctgcagcac gttgttgacc gccgaccagg cgtcggcggc
     1681 gttcgcgaag aagttgtcga tgctgcgccg cacgaactgc ggcacgacct tcgcgtagcc
     1741 ggtcgcgatc ggcttgagga tcttctcgtc cagcccctcg ttgaacgcga agaccttgcg
     1801 gttccagttc tcccacgggt cgaggcgcgc gcccgggccg ccgcgcgcat cgttcacgag
     1861 ggcctgcgtg gtggcgcagc cgccgagcac gagcagcgcg cccagcagca acggcagggt
     1921 tcgcgcgcgg ggacggtgcg tggcgggtga gcgggtcact tcttgcctcc attcgcggcc
     1981 ggtgccggcg tcgacgtgga accggcgtcc gcggccttgc tgaacaggaa ctggctgatc
     2041 aggctttcga gcacgatcgc cgattgggtt tgcttgatgg tatcgccggc ggcgaaattc
     2101 ttctcgctcg cgccggcttc gaggcccacg tactggtcgc cgagcaggcc cgaggtcagg
     2161 atgtgcgccg acgagtcgga cggaaactgc accgcacgat cgatctcgat ccgcaccgcg
     2221 ccctggtagc gcttcgcgtc gaggctgatc gaggcgaccc ggccgacggt gacgccggcg
     2281 ctgc
//
LOCUS       DASRDG010000117         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_821293, whole genome shotgun sequence.
ACCESSION   DASRDG010000117 DASRDG010000000
VERSION     DASRDG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821293"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFA35_01960"
     CDS             <1..590
                     /locus_tag="VFA35_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZT54962.1"
                     /translation="MIVLIGYAIPGFVLGLFLIVLFAGGTFFEWFPLRGLTSDNWAAL
                     SWPARIADYFWHITLPLICMVIGSFAIVTMLTKNTFVEEMRKQYVLVARAKGLSQRRV
                     LYKHIFRNALIPLITGFPAAFVGAFFAGSVLIETLFSLDGLGLLSYESVVRRDYPVVL
                     GSLYLFTLIGLVVKLIADLLYVLVDPRVQFSSVGK"
     gene            590..1717
                     /locus_tag="VFA35_01965"
     CDS             590..1717
                     /locus_tag="VFA35_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT54963.1"
                     /translation="MVTDETFDAAAGPAPGRSQAGPHPLGGSTDVPVGRGAPLSPNRR
                     AWARFKRNRLGYWSLWIFGILLFLATFAELISNDRPLLARYDGELVVPLVNNPPETRF
                     GGDFGTPTDWKDPLIRAQFAKPGNWALFTFNEHSDQSQDYFLKAPAPSAPSAQNLLGT
                     DGDSHDMVAKLIYGFRVSIWFAFALTLVGTVLGILAGAVQGYFGGRTDLFLQRIFEIW
                     GAVPELYLLIIFASIFEPSMLLLLVLLSLWGWLGLSDYVRAEFLRNRGLEFVKAARAL
                     GLSNRQIIWRHVLPNSLTPVITFLPFRMSAAIIALTSLDFLGLGVPQGTPSLGELLRQ
                     GKANLDAWWIIFPTFAVLVVTMLLLTFIGDALRDAFDTRKS"
     gene            1714..3306
                     /locus_tag="VFA35_01970"
     CDS             1714..3306
                     /locus_tag="VFA35_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT54964.1"
                     /translation="MSTTPLLSIEHLTVRFGASTVVDDVSFAVREGEKFALVGESGSG
                     KSITALSVLRLVDAAVTSGVIRFDGDDLVEKSERQMRGIRGADIAMIFQEPMTALNPL
                     YTVGNQIGEVLELHEGLRKNQARERAIELLRRTGIPEPERRVDAFPHQLSGGQRQRAM
                     IAMALACRPKLLICDEPTTALDVTIQAQILALLDELQAEMHMALLFITHDLNLVRRFT
                     HRVGVMERGKLVETGLTTAVFAHPQHPYTRKLLASRPKRVVEPLAADAPVLLAADAVG
                     VNFTIAQGWFGKRVFEAVRRATLQLKRGETLGIVGESGSGKTTLGMALLALQPIASGA
                     IEMGGQRIDNADRGVLRAMRKRMQVVFQDPFASLSPRMTVGQIVGEGLALHRPELTLS
                     ARDKLVLEMLAEVGLSEAKGVANVLQRYPHEFSGGQRQRIAIARAVVLRPEVLVLDEP
                     TSALDVSVQQQVLKLLAELQRHYGLSYVFISHDLAVVRAMSHRVMVMKNGDVIEEGEA
                     QALFDAPREPYTKALLAAAHLA"
     gene            3320..3925
                     /locus_tag="VFA35_01975"
     CDS             3320..3925
                     /locus_tag="VFA35_01975"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011458668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HZT54965.1"
                     /translation="MAKLHFRYAAMNAGKSTALLQAAHNYEERGMRVRLFTAALDDRD
                     GRGVIASRLGLAREAETFGPETEFVRSHLGPLDDSIACLLIDEAQFLTLEQVRQLHRL
                     AHVDAVPVVCYGLRSDFRAEAFAGAAALLTLADDIEEMKSVCACGRKATLNLRIDARG
                     QRVREGEQVFIGGNDRYRAICPACFYKDDEHGPDAAPGLFA"
BASE COUNT          567 a         1388 c         1372 g          658 t
ORIGIN      
        1 cgatgatcgt gctgatcggc tacgcgattc ccggcttcgt gctcggcctg ttcctgatcg
       61 tgctgttcgc gggcggcacc ttcttcgaat ggttcccgct gcgcggcctc acgtccgaca
      121 actgggccgc gctgagctgg ccggcgcgca tcgccgacta cttctggcac atcacgctgc
      181 cgctgatctg catggtgatc ggcagcttcg cgatcgtcac gatgctgacc aagaacacct
      241 tcgtcgagga gatgcgcaag cagtacgtgc tggtcgcgcg cgcgaagggg ctgtcgcagc
      301 ggcgcgtgct ctacaagcac atcttccgca acgcgctgat cccgctgatc accggcttcc
      361 cggcggcgtt cgtcggcgcg ttcttcgccg gctcggtgct gatcgagacg ctgttctcgc
      421 tcgacggcct ggggctgctg tcgtacgaat cggtggtgcg gcgcgactac ccggtcgtgc
      481 tgggctcgct ctacctgttc acgctgatcg gcctggtcgt caagctgatc gccgacctgc
      541 tgtacgtgct ggtcgatccg cgcgtgcagt tcagcagcgt gggcaagtga tggtgactga
      601 cgagaccttc gacgccgcgg cgggcccggc gccgggccgc tcccaagcgg gcccacatcc
      661 cctcggggga tcgaccgacg tacccgtcgg gcgaggggcc ccactttcac cgaaccggcg
      721 tgcctgggcg cgcttcaagc gcaaccggct cggctactgg tcgctgtgga tcttcggcat
      781 cctgctgttc ctcgcgacct tcgcggaact catcagcaac gaccggccgc tgctcgcgcg
      841 ctacgacggc gaactggtcg tgccgctcgt caacaacccg cccgaaaccc gcttcggcgg
      901 cgacttcggc acgccgaccg actggaagga cccgctgatc cgcgcgcagt tcgccaagcc
      961 cggcaactgg gcgctgttca cgttcaacga gcactccgac cagtcgcagg actacttcct
     1021 gaaagcgccg gcgccgtcgg cgccgagcgc acagaacctg ctcggcaccg acggcgactc
     1081 gcacgacatg gtcgcgaagc tgatctacgg ctttcgcgtc agcatctggt tcgcgttcgc
     1141 gctgacgctg gtgggcaccg tgctcggcat cctcgccggc gccgtgcagg gctacttcgg
     1201 cggacgcacc gacctgttcc tgcagcgcat cttcgagatc tggggcgcgg tgccggagct
     1261 gtacctgctg atcatcttcg cgtcgatctt cgagccctcg atgctgctgc tgctggtgct
     1321 gctgtcgctg tggggctggc tcgggctgtc ggactacgtg cgtgccgagt tcctgcgcaa
     1381 tcgcgggctc gagttcgtca aggcggcgcg cgcgctcggc ctgtcgaacc gccagatcat
     1441 ctggcgccac gtgctgccga actcgctgac gccggtgatc accttcctgc cgttccgcat
     1501 gagcgcggcg atcatcgcgc tgacctcgct cgactttctc ggcctgggcg tgccgcaggg
     1561 cacgccgtcg ctcggcgagc tgctgcgcca gggcaaggcc aacctcgacg cctggtggat
     1621 catcttcccg accttcgcgg tgctggtcgt gacgatgctg ctgctgacct tcatcggcga
     1681 cgcgctgcgc gacgccttcg acacgcgcaa gtcatgagca cgacgcctct gctgtcgatc
     1741 gagcacctga cggtgcgctt cggcgcgagc accgtcgtcg acgacgtgtc gttcgcggtt
     1801 cgcgagggcg agaagttcgc gctggtcggc gaatcgggct cgggcaaatc gatcaccgcg
     1861 ctgtcggtgc tgcggctggt cgacgcggcg gtgacgagcg gcgtgatccg cttcgacggc
     1921 gacgatctgg tcgagaagtc ggaacgtcag atgcgcggca tccgcggcgc cgacatcgcg
     1981 atgatcttcc aggagccgat gacggcgctg aacccgctgt acacggtggg caaccagatc
     2041 ggcgaggtgc tggagctgca cgagggcctg cgcaagaacc aggcgcgcga gcgcgcgatc
     2101 gagctgctgc ggcgcaccgg catccccgag ccggagcgcc gcgtcgacgc gttcccgcac
     2161 cagctctcgg gcggccagcg ccagcgcgcg atgatcgcga tggcgttagc ctgccgaccg
     2221 aagctgctga tctgcgacga gccgacgacc gcgctcgacg tgacgattca ggcgcagatc
     2281 ctcgcgctgc tcgacgagtt gcaggccgag atgcacatgg cgctgctgtt catcacccac
     2341 gacctgaacc tggtgcggcg cttcacgcac cgcgtcggcg tgatggagcg cggcaagctg
     2401 gttgagaccg gcctgacgac cgcggtgttc gcgcacccgc agcaccccta cacgcgcaag
     2461 ctgctggcaa gccggccgaa gcgcgtcgtc gagccgctcg ccgcggatgc gccggtgctg
     2521 ctcgccgccg acgctgtcgg cgtgaacttc acgatcgcgc agggctggtt cggcaagcgc
     2581 gtgttcgagg cggtgcgccg cgccacgctg cagctcaagc gcggcgagac gctcggcatc
     2641 gtcggcgaat cgggttcggg caagacgacg ctgggcatgg cgctgctggc gctgcagccg
     2701 atcgcctccg gcgcgatcga gatgggcggc cagcgcatcg acaacgccga ccgcggcgtg
     2761 ctgcgcgcga tgcgcaagcg catgcaggtg gtgttccagg acccgttcgc gtcgctcagc
     2821 ccgcgcatga cggtcggcca gatcgtcggc gagggcctgg cgctgcaccg gcccgagctg
     2881 acgctgtcgg cacgcgacaa gctcgtgctc gagatgctcg ccgaggtcgg cctgagcgag
     2941 gcgaaaggcg tggcgaatgt gctgcagcgc tacccgcacg agttctcggg cggccagcgc
     3001 cagcgcatcg cgatcgcgcg cgccgtggtg ctgcggcccg aggtgctggt gctcgacgag
     3061 ccgacctcgg cgctcgacgt gtcggtccag cagcaggtgc tgaagctgct cgccgaactg
     3121 cagcgccatt acggcctgag ctacgtgttc atcagccacg acctcgcggt ggtgcgcgcg
     3181 atgtcgcacc gcgtgatggt gatgaagaac ggcgacgtga tcgaggaggg cgaggcgcag
     3241 gcgctgttcg acgcgccgcg cgagccctac accaaggcct tgctggccgc cgcccacctg
     3301 gcctgagtcg cgcaccgcca tggccaagtt gcacttccgc tacgcggcga tgaacgcggg
     3361 caagtcgacc gcgctgctgc aggcggcgca caactacgaa gagcgcggca tgcgggtgcg
     3421 cctgttcacc gccgcgctcg acgaccgcga cggccgcggc gtgatcgcct cgcgcctggg
     3481 gctcgcgcgc gaggccgaga ccttcggccc cgagaccgag ttcgtgcgca gccacctcgg
     3541 cccactcgac gacagcatcg cctgcctgct gatcgacgag gcgcagttcc tcacgctcga
     3601 gcaggtgcgc cagctgcacc ggctggcgca tgtcgacgcc gtgccggtgg tctgctacgg
     3661 cctgcgcagc gacttccgcg ccgaggcctt cgccggcgct gccgcgctgc tgaccctggc
     3721 cgacgacatc gaggagatga agtcggtttg cgcctgcggc cgcaaggcga cgctgaacct
     3781 gcgcatcgac gcgcgcggcc agcgcgtgcg tgagggcgag caggtgttca tcggcggcaa
     3841 cgaccgctac cgcgcgatct gcccggcctg cttctacaag gacgacgagc acgggcccga
     3901 cgccgcgccg gggctgttcg cctgacggcg gcccggcgcc gcgcgcgtca cttcagcagg
     3961 ccttcggcct tcatcgccgc ctgcg
//
LOCUS       DASRDG010000118         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_476720, whole genome shotgun sequence.
ACCESSION   DASRDG010000118 DASRDG010000000
VERSION     DASRDG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476720"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /gene="glmM"
                     /locus_tag="VFA35_01980"
     CDS             <1..797
                     /gene="glmM"
                     /locus_tag="VFA35_01980"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZT54966.1"
                     /translation="GSQLVPAALRAFGADVVEVACAPDDFNINDGVGALHPEVAAAAV
                     REHGAHLGICLDGDGDRGIFVDETGVVRDGDDQLCLFGLHLHAQRRLPRATVVATVMS
                     NLGLHRALAAAGIAVHVTAVGDRNVVLAMKQHGYALGGEQSGHLLFAGDGHLTGDGLF
                     TALQLLSIPGAREHGFARLFASFRRFPQQLVNVHVARKPPFDTVPAIAAKAAAIERQL
                     GADGRLVLRYSGTEPLCRVMIEGADGAVVQQLAHELAALIAAELGA"
     gene            794..1480
                     /locus_tag="VFA35_01985"
     CDS             794..1480
                     /locus_tag="VFA35_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54967.1"
                     /translation="MNGDDDALPALAVSGSGVDGVQFHAAVRTAGGIAAASAPAHQRC
                     DLATLVQQTLRAAGTAPAAVRELRVDRGPGSYTGLRVAVTFARAWCVFGGARLRSVDT
                     LSLFAAAALAGAPELGERRLVPVLAGQQDRVHAAVLRRGRDGALVASSPPALLPLARL
                     RDVIGRDDVLIAAPALIPALAPLAASAGVRTHAVAAVGAEHLFSPALAPERADPVALE
                     PLYLMGSYVG"
     gene            complement(1644..>2106)
                     /locus_tag="VFA35_01990"
     CDS             complement(1644..>2106)
                     /locus_tag="VFA35_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54968.1"
                     /translation="QGLQSGARCEDLRRQRTRCLGSARSSAAGGRRARTGNAEIAVPG
                     PISARIQLDRVWRAAKLRADRGGAGTCSAASDRYLFLFPQESTMAKKAKKGPSGDSMI
                     ISKSRTKAAVKKCNVSSDFYGALDGAVRTMIARAEIRADANGRKTVRPQDL"
BASE COUNT          255 a          785 c          765 g          301 t
ORIGIN      
        1 gcggctcgca gctcgtgccg gcggcgctgc gcgcgttcgg cgccgacgtc gtcgaggtcg
       61 cgtgcgcgcc cgacgacttc aacatcaacg acggcgtcgg cgccctgcac cccgaggtcg
      121 cggccgccgc cgtgcgcgaa cacggcgcgc acctgggcat ctgcctcgac ggggacggcg
      181 accgcggcat cttcgtcgac gagaccggcg tcgtgcgcga cggcgacgac cagctctgcc
      241 tgttcggact gcacctgcac gcgcagcgcc gcctgccgcg cgcgacggtc gtggcgacgg
      301 tgatgagcaa cctcgggctg catcgcgcgc tcgcggcggc gggcatcgcc gtgcacgtga
      361 cggcggtcgg cgaccgcaac gtggtgctgg cgatgaagca gcacggctac gcgctcggcg
      421 gcgagcagtc gggtcacctg ctgttcgccg gcgacggcca cctgaccggc gacggcctgt
      481 tcacggcgct gcagctgctg tcgatcccgg gcgcgcgcga gcacgggttc gcgcgcttgt
      541 tcgcgtcgtt ccgccgcttc ccgcagcagc tcgtcaacgt gcacgtcgcg cggaagccgc
      601 cgttcgacac tgtgcccgcg atcgccgcga aggcggcggc gatcgagcgg cagctgggcg
      661 ccgacggccg cctcgtgctg cgctactcgg gcaccgaacc gctgtgccgc gtcatgatcg
      721 aaggcgccga cggcgcggtc gtgcagcagc tcgcgcacga actcgccgcg ctgatcgccg
      781 cggagctcgg cgcgtgaacg gcgacgacga cgcgctgccg gcgctggccg tgagcggcag
      841 cggcgtcgac ggggtgcagt tccacgccgc cgtgcgcacg gcgggcggaa tcgccgcggc
      901 gagtgcgccg gcccatcagc gctgcgacct cgcgactctc gtgcagcaga cgctgcgcgc
      961 cgccggcacg gcacccgccg cggtgcgcga actgcgcgtc gaccgcggcc ccggctccta
     1021 caccggcctg cgggtcgcgg tcacgttcgc gcgcgcgtgg tgcgtgttcg gcggcgcgcg
     1081 cctgcgcagc gtcgacacgc tgtcgctgtt cgccgcggcg gcgctcgcgg gcgcgcccga
     1141 gctcggcgaa cggcggctcg tgccggtgct cgcgggccag caggaccgcg tgcacgcagc
     1201 ggtgctgcga cgcgggcgcg acggcgcgct ggtcgcgtcg tcgccgcccg cgctgctgcc
     1261 gctcgcccga ttgcgcgacg tgatcgggcg cgacgacgtg ctgatcgcgg cgccggcgct
     1321 gatccccgcg ctcgcgccgc tcgccgccag tgccggcgtg cgcacgcacg cggtcgcggc
     1381 ggtcggcgcg gagcacctgt tctcgccggc gctcgccccc gaacgcgcgg atcccgtggc
     1441 gctcgagccg ctctacctga tgggctccta cgtcggctga ggcgaacacg ccgaaggcgg
     1501 gttcgcctcg ggattccttc tcactctccg agtcgcggac ggcgaccggg agacgccgcg
     1561 gaccgatcgc gaaaaggaaa cggcccgcaa cacgggccgt tcctcgtcac ctgggaccca
     1621 gcagtcccgt tacgaccggc gaactagaga tcctgcgggc ggacggtctt gcgaccgttc
     1681 gcgtcggcgc ggatctcggc gcgcgcgatc atcgtgcgca cggcgccgtc gagcgcgccg
     1741 tagaagtcgg acgacacgtt gcacttcttg accgcggcct tcgtgcgcga cttgctgatg
     1801 atcatgctgt cgccgctggg gcccttcttt gctttcttcg ccatcgttga ctcttgggga
     1861 aagaggaaaa ggtagcgatc ggacgccgcg gaacacgttc ccgcgccgcc acgatcggcg
     1921 cgcagtttgg cggcacgcca gaccctgtca agctgaattc gcgccgaaat cggccctgga
     1981 accgcgatct cggcatttcc cgtccgagcc cggcggccgc ccgcggcgct gcttctcgcc
     2041 gatccgaggc acctggtcct ctgcctgcgc aagtcctcgc agcgcgcgcc ggactgcaac
     2101 ccttgg
//
LOCUS       DASRDG010000119         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_375853, whole genome shotgun sequence.
ACCESSION   DASRDG010000119 DASRDG010000000
VERSION     DASRDG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375853"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..789
                     /locus_tag="VFA35_01995"
     CDS             40..789
                     /locus_tag="VFA35_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZT54969.1"
                     /translation="MSTTHDLVTALKAELKAAGITYADLAGQLGMAESSIKRIFAKGD
                     MPLSRIDEVLRVLKMDFAELARKIADAQPLRRELTLEQETAVVADRKLMLLAICCLSQ
                     WTFEQMVATYAISEAECVRYLLQLDKLGFIELRPLNRYRLKVGKGFRWRPHGPAMAYF
                     REHVTADYYSGGFDGEGETLMLVHGHISTAQAVNFVERLQRVGQDFAQQHLTDQKLAA
                     EQKGHYTLVVGMRSWLFGPFRDLKRAAARPA"
     gene            complement(802..1644)
                     /gene="rlmJ"
                     /locus_tag="VFA35_02000"
     CDS             complement(802..1644)
                     /gene="rlmJ"
                     /locus_tag="VFA35_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HZT54970.1"
                     /translation="MLAYRHAFHAGNHADVLKHTVLTLVLRYMNHKDKPYRLVDTHAG
                     AGGYSLEGRFAQKKGEFEQGILRLWDRDDLPPAVADYVQLVRQFNPDGLLEQYPGSPA
                     FAQMLLRAQDQLRLFELHPTDHRILQSYLGEVKGAEVFDTDGFDGLKGQVPPSSRRAV
                     VLMDPSYEGHKDYGRVITALREAIARFAEGVYVVWYPQVSKLEAAQLPKRLEALAPKG
                     WLHARLTVQQPDQQGFGLAGSGMFVINPPFTLHDEMLKVLPYLTDVLGQYDGANYLLE
                     QRPA"
     gene            1792..2895
                     /locus_tag="VFA35_02005"
     CDS             1792..2895
                     /locus_tag="VFA35_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HZT54971.1"
                     /translation="MTTLFDPIRLGDITAANRIVMAPLTRDRAGPGQVPTTLMAAYYA
                     QRAGAGLIVSEGTQISAEGQGYLDTPGIYSAEQVAGWRNITDAVHARGGKIVAQLWHV
                     GRISHLSLLPPGKQPVSSTAKRAHGKTFTANGFEDVAAPRALRLDEIPRLVEDYAQAA
                     RNAIDAGFDGVEVHGANGYLLEQFMRDSINDRSDAYGGAPENRARLVVEVMRAIADEI
                     GAGRAGIRLSPVTPANDAGPDSDAQGTYNLVVERLATLKLAFIHVVEGATGGPRDIAP
                     FDFAALRERFKRGHPQGAWIVNNGYTREMAIAAVESGAADMVAFGKAFISNPDLVERL
                     RRDAPLAPLQRETLYGGGAEGYTDYPTLQTAPA"
     gene            complement(2985..>3312)
                     /gene="lysM"
                     /locus_tag="VFA35_02010"
     CDS             complement(2985..>3312)
                     /gene="lysM"
                     /locus_tag="VFA35_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HZT54972.1"
                     /translation="VDGLDVAFDGATGTVSVAGEAADQATKEKVILCCGNVAGVTQVD
                     DAMTVAEPADASRWYTVVRGDTLSAIAKSQYGNANEYPKIFEANKPMLSSPDKIYPGQ
                     VLRIPA"
BASE COUNT          519 a         1160 c         1133 g          500 t
ORIGIN      
        1 cgtctggtat cgtttcaagg tacttttcgc cgagccccga tgagcacgac gcacgacctc
       61 gtcaccgcgc tgaaggccga actgaaggcc gccggcatca cctacgccga tctcgccggg
      121 cagctcggca tggccgagtc gagcatcaag cgcatcttcg cgaagggcga catgccgctg
      181 tcgcgcatcg acgaggtgct gcgcgtgctg aagatggatt tcgcggagct ggcgcgcaag
      241 atcgccgacg cccagccgct gcgccgcgag ctcacgctcg agcaggaaac cgcggtcgtc
      301 gccgaccgca agctgatgct gctggcgatc tgctgcctga gccagtggac cttcgagcag
      361 atggtcgcga cctacgcgat cagcgaggcc gagtgcgtgc gctacctgct gcagctcgac
      421 aagctcggct tcatcgaact gcgcccgctg aatcgctacc ggctgaaggt cggcaagggc
      481 tttcgctggc gcccgcacgg gccggcgatg gcctacttcc gcgagcacgt caccgccgac
      541 tactacagcg gcggcttcga cggcgaaggc gagacgctga tgctggtgca cggccacatc
      601 agcaccgcgc aggcggtcaa cttcgtcgag cggctgcaac gcgtcgggca ggacttcgcg
      661 cagcagcacc tgaccgacca gaagctcgcg gcggagcaga agggccacta cacgctggtg
      721 gtcggcatgc gctcgtggct gttcggcccg ttccgcgatc tgaagcgcgc ggcggcgcga
      781 cccgcctgac cgggcggctc gtcaggcggg cctctgctcc agcaggtagt tcgcgccgtc
      841 gtactggccg agcacgtcgg tcaggtaggg caggaccttc agcatctcgt catgcaaggt
      901 gaacggcggg ttgatcacga acatgccgct gccggccagg ccgaagccct gttggtcggg
      961 ctgctgcacc gtcagccgcg cgtgcagcca gccctttggc gcgagcgctt cgaggcgctt
     1021 cggcagttgc gccgcttcga gcttgctgac ctgcgggtac cagacgacgt acacgccctc
     1081 ggcgaagcgc gcgatcgcct cgcgcagcgc ggtgatcacg cggccgtagt ccttgtggcc
     1141 ctcgtagctc gggtccatca gcaccaccgc ccggcgcgac gagggcggca cttggccctt
     1201 caggccgtcg aagccatcgg tgtcgaagac ctccgcgcct ttcacctcgc cgaggtacga
     1261 ctgcaggatg cggtggtcgg tcgggtgcag ctcgaacagg cgcagctggt cctgcgcgcg
     1321 caacagcatc tgcgcgaaag ccggcgagcc ggggtactgc tcgagcagcc cgtcgggatt
     1381 gaactggcgc acgagctgca cgtagtcggc caccgccggc ggcaggtcgt cacgatccca
     1441 cagccgcagg atgccctgtt cgaactcgcc cttcttctgc gcgaagcgcc cttcgagcga
     1501 atagccgccc gcgccggcgt gggtgtcgac gagccggtag ggcttgtcct tgtggttcat
     1561 gtagcgcagc accagcgtca gcacggtgtg cttgagcacg tcggcgtggt tccccgcgtg
     1621 gaaggcgtgt cggtaggcga gcatggcgct agtgtcgtcg gattcgggct ggcactgtag
     1681 cggctgcggc cggcgcgaat cgtcctaacc gatttcggcg ggggctgcgc gccgtcctgt
     1741 tattcggggc ggccgtagca tcgcccgcac ccagcccaca cgaggacccg catgaccacg
     1801 ctcttcgacc cgatccgcct cggcgacatc accgccgcca accgcatcgt gatggcgccg
     1861 ctgacgcgcg accgcgccgg cccggggcag gtgcccacaa cgttgatggc agcctactac
     1921 gcgcagcgcg ccggtgccgg gctgatcgtc agcgaaggca cgcagatcag cgccgagggc
     1981 cagggctatc tcgacacacc gggcatctac agcgccgaac aggtcgccgg ctggaggaac
     2041 atcaccgatg ccgtgcacgc gagaggcggc aagatcgtcg cgcaactgtg gcatgtcggg
     2101 cgcatctcgc acctgtcgct gctgccgccg ggcaagcagc cggtgtcgag caccgcgaag
     2161 cgcgcgcacg gcaagacctt caccgcgaat ggtttcgagg acgtggcggc gccgcgcgcg
     2221 ctgcgcctcg acgagatccc gcgcctggtc gaagactacg cccaagctgc gcgcaacgcg
     2281 atcgacgccg gcttcgacgg cgtcgaggtg cacggcgcga acggctacct gctcgagcag
     2341 ttcatgcgcg acagcatcaa cgaccgctcc gatgcctacg gcggcgcgcc cgagaaccgc
     2401 gcgcgcctcg tcgtcgaggt gatgcgtgcg atcgccgacg agatcggcgc cggccgcgcc
     2461 ggtatccggc tctcgccggt gacgccggcc aacgacgccg ggcccgacag cgacgcgcaa
     2521 ggcacctaca acctcgtggt cgagaggctg gcaacgctga agctcgcgtt catccacgtc
     2581 gtcgaaggtg cgaccggcgg tccgcgcgac atcgcgccgt tcgacttcgc cgcactgcgc
     2641 gaacgcttca agcgcggcca cccgcaaggc gcgtggatcg tcaacaacgg ctacacgcgc
     2701 gagatggcga tcgcggcggt ggagagcggc gcggccgaca tggtcgcgtt cggcaaggcc
     2761 ttcatcagca acccggacct ggtcgagcgc ctcaggcgcg atgccccgct cgcgccgctc
     2821 cagcgcgaga cgctgtacgg cggcggcgcc gagggctaca ccgactaccc gaccttgcag
     2881 accgcgcccg cctgatcgcg gcgggccgag cgccgggccg ccccaagccg gcccgcatcc
     2941 cctcggggga tcggccgcgg tacgccgcga ccgaggggct ccattcaagc cgggatgcgc
     3001 agcacctggc cggggtagat cttgtccggg ctcgacagca tcggcttgtt ggcctcgaag
     3061 atctttgggt actcgttggc gttgccgtac tggcttttcg cgatcgccga cagcgtgtcg
     3121 ccgcgcacga cggtgtacca gcgcgatgcg tcggccggtt cggccacggt catcgcatcg
     3181 tcgacctgcg tcacgccggc gacgttgccg cagcacagga tcaccttctc cttggtggcc
     3241 tggtcggccg cctcgcccgc gaccgagacg gtgccggtcg cgccgtcgaa tgcgacatcg
     3301 aggccgtcca cc
//
LOCUS       DASRDG010000120         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_821449, whole genome shotgun sequence.
ACCESSION   DASRDG010000120 DASRDG010000000
VERSION     DASRDG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821449"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2290)
                     /locus_tag="VFA35_02015"
     CDS             complement(<1..2290)
                     /locus_tag="VFA35_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HZT54973.1"
                     /translation="MNTALASPNETRSTCPYCGVGCGVIIETQADQITGVRGDPEHPA
                     NFGRLCTKGSTLHLTATRTIAMQARLLQPQLRESRDEPRSVVGWDTALDTAANRFASI
                     VHAHGPDAVGFYLSGQLLTEDYYVFNKLAKGLIGTNNVDTNSRLCMSSAVAGYKQTLG
                     ADAPPACYDDLNHAETLFIVGSNTTFAHPILYRRIEDAKRANPLLRMIVADPRRTETA
                     DAADLFLQILPGTDVALFNGMLHVMLWEGWLDAAYIAAHTNGFDALKTRVRDFTPKDV
                     AQMCGIGEADLIQAARWFAGLDASFGSRKPVLSLYCQGLNQSSSGTAKNAALINLHLA
                     TGQIGKPGAGPFSLTGQPNAMGGREVGGLSNQLSAHRDLSNAAHRAEVARLWNVADVP
                     AKPGKSAVEMFEAAADGEIKALWIACTNPAQSMPNASTVRRALEVAEFVVVQEAFATT
                     TTCAYADLLLPASTWGEKDGTVTNSERRISRVRASVPAVGEARDDWRIAAEFARRLEA
                     RLQPGGPGRFAYPTPESVWNEHRESTRGRDLDITGLSYAALEVAPQQWPFPAGASTGR
                     ARLYEDGLYPTDDGRATFVDTPYVKLAEPRDVRYPFSLNTGRLRDQWHGMSRTGTIGR
                     LFGHVPEPAIEMHGQDMQRLALKDGDLVQVTSKRGSIVLPVRASEQQGLAQAFIPMHW
                     GDEFLSGSTATGQRVAGVNALTLPTFCPSSKQPELKHAAVKILKAELPWRLLCVAWLS
                     GDDALRAREQLKPLMQAFPFAAC"
     gene            complement(2287..2643)
                     /gene="nirD"
                     /locus_tag="VFA35_02020"
     CDS             complement(2287..2643)
                     /gene="nirD"
                     /locus_tag="VFA35_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HZT54974.1"
                     /translation="MNNWKSICKVEDIPVLGARRVDRATGGAIAIFRNAEDKVFALLD
                     SCPHKGGPLSQGIVFGDSVACPLHNWTIGLDDGCARSPDEGCTPRFSVKVDGGTVHLD
                     LDELNSIALGEVSAAT"
     gene            complement(2649..>2864)
                     /locus_tag="VFA35_02025"
     CDS             complement(2649..>2864)
                     /locus_tag="VFA35_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54975.1"
                     /translation="AHDELRKAPETTPAIKAELELAETQFTFFDTALRTLRPGAPDAS
                     HMANVFTTSERILQVMDGVTGMYSKLS"
BASE COUNT          380 a          958 c         1048 g          478 t
ORIGIN      
        1 cgcaggcggc gaacgggaac gcctgcatca gcggcttcag ctgctcgcgc gcacgcagcg
       61 catcgtcgcc gctgagccag gccacgcaca acagcctcca cggcagctcg gccttcagga
      121 tcttcaccgc cgcgtgcttc agctcgggct gcttcgaact cgggcagaag gtgggcaagg
      181 tcaacgcgtt gacgcccgcc acgcgctggc cggtcgcggt gctgccggag aggaactcat
      241 cgccccagtg catcgggatg aacgcctgcg cgaggccctg ctgttcgctg gcgcgcaccg
      301 gcagcacgat cgacccgcgc ttggacgtga cctgcaccag gtcgccgtcc ttgagcgcga
      361 ggcgctgcat gtcctgcccg tgcatctcga tcgccggctc cggcacgtgg ccgaacagcc
      421 gtccgatcgt gccggtgcgg ctcatgccgt gccactggtc gcgcaggcgg ccggtgttga
      481 gcgagaacgg gtagcgcacg tcgcgcggtt cggcgagctt gacgtagggc gtgtcgacga
      541 aggtcgcgcg gccgtcgtcg gtcgggtaca ggccgtcttc gtagagccgg gcgcggccag
      601 tcgacgcacc cgcggggaag ggccactgct gtggcgcgac ttccagcgcg gcatagctca
      661 ggccggtgat gtcgaggtcg cggccgcggg tcgattcgcg gtgctcgttc cagaccgact
      721 cgggcgttgg gtacgcgaac cggcccgggc cgccgggctg caggcgcgcc tcgagccggc
      781 gcgcgaactc ggccgcgatg cgccagtcgt cgcgcgcctc gccgaccgcg ggcaccgatg
      841 cgcgcacccg gctgatgcgg cgctcgctgt tggtcacggt gccgtccttc tcgccccagg
      901 tcgacgcggg cagcagcagg tcggcatagg cgcaggtcgt ggtcgtcgcg aaggcctcct
      961 gcacgacgac gaactcggcg acttcgagcg cgcgccgcac ggtggacgca ttgggcatcg
     1021 actgtgccgg gttcgtgcag gcgatccaca gcgccttgat ctcgccgtcg gcggccgcct
     1081 cgaacatctc gaccgccgac ttgcccggct tcgcgggcac gtcggcgacg ttccacagcc
     1141 gcgccacctc ggcgcggtgc gcggcgttcg agagatcgcg gtgggcgctc agctggttcg
     1201 agaggccgcc gacctcgcgg ccgcccatcg cgttcggttg cccggtcagc gagaacgggc
     1261 ccgcgcccgg cttgccgatc tggccggtgg cgaggtgcag gttgatcagc gcggcgttct
     1321 tcgcggtccc gctgctgctc tggttcaggc cctggcagta cagcgagagc accggcttgc
     1381 gcgatccgaa cgacgcgtcg agcccggcga accagcgtgc ggcctggatc aggtcggcct
     1441 cgccgatgcc gcacatctgc gccacgtcct tgggcgtgaa gtcgcgcacg cgcgtcttca
     1501 gcgcatcgaa cccgttggtg tgcgcggcga tgtaggcggc gtcgagccag ccttcccaga
     1561 gcatcacgtg cagcatgccg ttgaacagcg caacgtcggt gccgggcagg atctgcagga
     1621 acaggtcggc cgcgtcggcc gtctcggtgc ggcgcgggtc ggcgacgatc atgcgcagca
     1681 gcgggttcgc gcgcttcgcg tcctcgatgc ggcgatacag gatcgggtgc gcgaacgtgg
     1741 tgttcgagcc gacgatgaac agcgtttcgg cgtggttcag gtcgtcgtag cacgcgggcg
     1801 gcgcatcggc gccgagcgtc tgcttgtagc ccgcgaccgc gctgctcatg cacaggcgcg
     1861 agttggtgtc gacgttgttc gtgccgatca ggcccttggc gagcttgttg aagacgtagt
     1921 agtcctcggt cagcagctgg cccgacaggt agaagccgac ggcgtcgggg ccgtgcgcgt
     1981 ggacgatgct cgcgaagcgg ttggcggcgg tgtcgagggc ggtgtcccag ccgaccacgc
     2041 tgcgcggctc gtcgcggctc tcgcgcagtt gcggctggag caggcgcgcc tgcatcgcga
     2101 tcgtgcgggt ggcggtcagg tgcagcgtgc tgcccttggt gcacaggcgc ccgaagttcg
     2161 ccgggtgctc ggggtcgccg cgcacgccgg tgatctgatc ggcctgcgtc tcgatgatga
     2221 cgccgcagcc gacgccgcag tacgggcagg tcgagcgcgt ttcgttcggc gacgccaagg
     2281 cggtgttcat gtcgccgccg acacctcgcc cagcgcgatg ctgttcagct cgtcgagatc
     2341 gagatgaacc gtgccgccgt cgaccttgac gctgaagcgc ggcgtgcagc cctcgtcggg
     2401 cgaacgcgcg cagccgtcgt ccaggccgat cgtccagttg tgcagcgggc aggcgacgct
     2461 gtcgccgaac acgatcccct ggctcaacgg gccgcccttg tgcgggcagc tgtcgagcag
     2521 cgcgaacacc ttgtcttcgg cgttgcggaa gatcgcgatc gcgccgccgg tggcgcgatc
     2581 gacgcggcgg gcgccgagga ccgggatgtc ctcgaccttg cagatgcttt tccagttgtt
     2641 catggcgttc agctcagttt cgaatacatg cccgtcacac cgtccatcac ctgcagaatg
     2701 cgttcgctgg tggtgaagac gttcgccatg tggctcgcgt cgggggcgcc cgggcgcagc
     2761 gtgcgcagcg cggtgtcgaa gaacgtgaac tgggtttcgg cgagctcgag ctcggccttg
     2821 atggccggcg tggtttcggg cgccttcctc agctcgtcgt gcgc
//
LOCUS       DASRDG010000121         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_253147, whole genome shotgun sequence.
ACCESSION   DASRDG010000121 DASRDG010000000
VERSION     DASRDG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253147"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="VFA35_02030"
     CDS             complement(<1..1398)
                     /locus_tag="VFA35_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZT54976.1"
                     /translation="MIALAFLNPLLLWGLPLAAVPIVIHLLNRRRFQRVPWAAMEYLL
                     AAMKRNRKRMRMEQWLVLLLRTLAVLLLISLVARPQLTGSGLIGARAHHVVLLDDSAS
                     MWQRSGSTTLHDKAREQIRQLAEYLATNRGGDLFSLVRTSHPSEPVVWGQRIGPDLAR
                     NVVLPKLKELPAGDGGMDLGAVLAATHKRALETKEAGRTEYWLVSDMRAVDWMTDDEK
                     PRASLQSALSAMDAKTAHLTTTAVGSRDADNLGIVAVRRLSRLSSAGVAVAFAVDVKN
                     YGLDAAPATELSVEVDGKSRVVQPVPPLAPGEAVPVTIVHTFHAAGFHRVDASLPATD
                     HFPIDDRRTLVLPVAERSKVLLVDGEPGETEEEGETRFLQVALDMGGDVVTGIEPQVI
                     TDANLADADLSPFDMVYLCNVSAPAPAVVDKLEAFVRGGGGLGFFVGAQVDPTRYNEL
                     FWKGGQGLLPLALGEL"
     gene            complement(1414..>2314)
                     /locus_tag="VFA35_02035"
     CDS             complement(1414..>2314)
                     /locus_tag="VFA35_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HZT54977.1"
                     /translation="AQSPTDGCWRSGGGTGSYPVAMSGLAGMALVASGSTPTRGRYWR
                     ALRQTCEFLLKHADPGSGVITVAQQEAQTMYGHGFATLFLASIYGMEEGADIQERLHG
                     VLTRAVRLIGQAQSGAGGWLYTPDSGGDEGSVTVTQVQALRACRMAGIVVDKRTIDRA
                     VDYIKKCQNADGGISYQLGTRGESRPAISAAGIAVLYNAGFYDDQPFVDKALKYVQKH
                     VNPTSDNTGHHFYTQLYYAQALYQRGGKDWDDYLAKFAPWLLRQQRPDGSWDSDGVGP
                     VYATAIGLIILQLPYALVPIYQR"
BASE COUNT          321 a          835 c          815 g          343 t
ORIGIN      
        1 cagttcgccg agcgcgagcg gcagcaggcc ctggccgccc ttccagaaca attcgttgta
       61 gcgggtcggg tcgacctgcg cgccgacgaa gaaaccgagc ccgccgccgc cgcgcacgaa
      121 cgcctcgagc ttgtcgacca ccgcgggcgc cggcgcgctg acgttgcaca ggtagaccat
      181 gtcgaacggc gacaggtcgg cgtccgcgag gttggcgtcg gtgatcacct gcggctcgat
      241 gccggtgacg acgtcgccgc ccatgtcgag cgcgacctgc aggaagcggg tctctccctc
      301 ctcctcggtc tcgcccggct cgccgtcgac gagcagcacc ttgctgcgct cggccaccgg
      361 cagcaccagc gtgcggcggt cgtcgatcgg gaagtggtcg gtcgccggca gcgacgcgtc
      421 gacgcggtgg aaacccgccg cgtggaacgt gtgcacgatc gtcaccggca ccgcctcgcc
      481 cggcgcgagc ggcggcaccg gctgcacgac gcggctcttg ccgtcgacct cgaccgacag
      541 ctccgtcgcc ggcgcggcgt cgaggccgta gttcttcacg tcgacggcga acgccaccgc
      601 gaccccggcc gagctcagcc gcgacaggcg tcgtaccgcg acgatgccca ggttgtcggc
      661 gtcgcgcgag ccgaccgcgg tcgtggtcag gtgcgccgtc ttcgcgtcca tcgccgacag
      721 cgccgactgc agcgacgcgc gcggcttctc gtcgtccgtc atccagtcga cggcgcgcat
      781 gtcgctcacg agccagtact cggtgcggcc cgcctccttc gtctcgagcg cgcgcttgtg
      841 cgtcgccgcg agcacggcgc cgaggtccat cccgccgtcg cccgcgggca gctccttgag
      901 cttcggcaac acgacgttgc gcgcgaggtc ggggccgatg cgctgtcccc acacgaccgg
      961 ctccgagggg tggctcgtgc gcacgagcga gaacaggtcg ccgccgcggt tggtcgccag
     1021 gtactcggcg agctggcgga tctgctcgcg cgccttgtcg tgcagcgtcg tgctgccgct
     1081 gcgctgccac atcgacgccg agtcgtcgag cagcacgacg tggtgcgcgc gcgcgccgat
     1141 caggccgctg cccgtgagct gcggccgcgc gaccagcgag atcagcagca gcacggcgag
     1201 cgtgcgcagc agcagcacga gccactgctc catgcgcatg cgcttgcggt tgcgcttcat
     1261 cgccgcgagc aggtactcca tcgccgccca cggcacgcgc tggaaccgcc gccggttcag
     1321 caggtggatg acgatcggca ccgcggcgag cggcaggccc cacagcagca gcgggttgag
     1381 gaaggcgagg gcgatcatgt cggggcccgg ccttcagcgc tggtagatgg ggaccagcgc
     1441 gtagggcagc tggaggatga tcaggccgat cgcagtggcg tagaccgggc cgacgccgtc
     1501 gctgtcccac gaaccgtcgg ggcgctgctg gcgcagcagc cacggcgcga acttggcgag
     1561 gtagtcgtcc cagtccttgc cgccgcgctg gtagagcgcc tgcgcgtagt agagctgcgt
     1621 gtagaagtgg tggcccgtgt tgtcggacgt cgggttcacg tgcttctgca cgtacttcag
     1681 cgccttgtcg acgaacggct ggtcgtcgta gaagcccgcg ttgtagagga ccgcgatgcc
     1741 ggcggcgctg atcgcgggac ggctctcgcc gcgcgtgccg agctgatagc tgatgccgcc
     1801 gtcggcgttc tggcacttct tgatgtaatc gacggcgcga tcgatcgtcc gcttgtcgac
     1861 gacgatgccc gccatgcggc acgcgcgcag cgcctgcacc tgcgtcaccg tcacgctgcc
     1921 ctcgtcgccg cccgagtcgg gcgtgtagag ccagccgccg gcgcccgact gcgcctgccc
     1981 gatcagcctg accgcgcgcg tgagcacgcc gtgcagccgc tcctggatgt ccgcgccctc
     2041 ctccatgccg tagatggacg ccaggaacag cgtcgcgaag ccgtgaccgt acatggtctg
     2101 ggcctcctgc tgcgccaccg tgatcacgcc agagccagga tcggcgtgct tcagcaggaa
     2161 ttcgcacgtc tgccgcaatg cgcgccagta cctgccgcgc gtcggcgtcg agcccgacgc
     2221 gaccagcgcc atgcccgcga gcccggacat cgccacggga tagctgccgg tgccgccgcc
     2281 gctgcgccag cagccgtcgg tcggcgactg ggcg
//
LOCUS       DASRDG010000122         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_20122, whole genome shotgun sequence.
ACCESSION   DASRDG010000122 DASRDG010000000
VERSION     DASRDG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20122"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="VFA35_02040"
     CDS             complement(<1..2201)
                     /locus_tag="VFA35_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54978.1"
                     /translation="MIGGDAIAFPFDTAGATWGLAGNRSTLCYVEGSAEVAAAIWSLP
                     SSNAPLEQAGEAAHGGSIALLLRQGPALRIAGASGVCRAVDSVLSANAGGLEWKLRRS
                     ETNLSIDVALWDPSSSRLESGAQPVMELRFASRRGGPDLVGLEGGRLHNRWNRPLAAS
                     DRPFAFDGAGAATTLIVERDGLQRTAASASRDPDTEVVEGYALENLYLHLQPARRAAF
                     VGSGVAPATLAEGHARLVFDVRFGQPMLPDPYASNWSVEHLNVRMADSALYADVAWSV
                     GAAPAVHPRLRAAVSFPQQPPDAARDDAARNRFRGVLDAERTHLALLDLSGRDQQFGL
                     AIEGVAESTPVIDADSRLVWPLRNVRLLLQPQVHWEPVHILANDKVLAKDEVVSSTSH
                     GGPTLIGAASVKLVPVLPGRVGDEIAAIANGQRRAAALFALPFGMHAFAFMEQRFEQD
                     LSPRPILALLHQPGFIEGPGDVFEAARQLRLIATGAGLQDGAANPSRTMPGLMTQTPN
                     LRPNANGLMSVLSSEVAGVVDATFANRVPLHRADLSGYGLSTFSRWRRELPPPPAKEG
                     TGVTQVRFDVLVGRTSYEVIELQSRLWTPQCRMVRTIIIERRNSGEVTRYDSGWNPIE
                     DGDCKRYANIETGVVKAFRNIRNVRITPRPLIEVDATWTWQEVRYDADLHLQPEPGKP
                     AADKLVPIRDHVGYIQIMPVDVIAANGEFDGPGTVPGPVEFDALMKALGHTV"
     gene            2214..>2679
                     /locus_tag="VFA35_02045"
     CDS             2214..>2679
                     /locus_tag="VFA35_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54979.1"
                     /translation="MRRETQCVTEPRQPERAADDRRDAIAQLDPGRQVDRQGIAVRSG
                     RFGGGRGFGLDAQRPRRREGHKQSSIGQWRRQDRTGVEDPASDAQGGEALCAGGQPIS
                     AYPDRSGGKVPAQRVGGIGRAGRRQQQDRFADLEEGCLQVRHDHDETHILEER"
BASE COUNT          420 a          935 c          937 g          387 t
ORIGIN      
        1 acggtatggc ccagtgcctt catcagcgca tcgaactcaa ccgggccggg cacggtgccg
       61 ggaccgtcga attcgccgtt cgcggcgatg acgtccaccg gcatgatctg gatgtagccg
      121 acgtgatcgc gaatcggcac cagcttgtcc gccgccggct tgccgggttc cggctgaagg
      181 tgcaggtcgg cgtcgtagcg gacctcctgc cacgtccacg tggcatccac ttcgatcagc
      241 ggtcggggcg tgatgcgtac gttgcggatg ttgcggaagg ccttgacgac accggtctcg
      301 atattcgcgt agcgcttgca gtcgccatcc tcgatcgggt tccagccgct gtcgtagcgt
      361 gtcacctcgc ccgagttgcg tcgttcgatg atgatcgtgc gcaccatgcg gcactgcggc
      421 gtccagagcc gggactgcag ctcgatgact tcgtacgacg tgcgcccgac cagcacgtcg
      481 aagcgtacct gcgtgacgcc ggtgccctcc ttcgcgggcg gcggcggcag ctcgcgccgc
      541 cagcggctga acgtgctcag gccgtagccc gagaggtcgg cgcggtgcag cggcacgcgg
      601 ttcgcgaagg tcgcgtcgac gaccccggcg acctcgctgc taagcacgct catgaggccg
      661 ttggcgttcg ggcgcaggtt cggcgtctgc gtcatgaggc ccggcatcgt gcgcgacggg
      721 ttcgccgcgc cgtcctgcag ccccgccccg gtagcgatca gccggagttg gcgagccgct
      781 tcgaacacgt cgccgggacc ctcgatgaac ccgggctggt gcaacagcgc caggatcggc
      841 cgagggctca agtcctgctc gaagcgctgc tccatgaacg caaacgcatg catcccgaaa
      901 ggcagcgcga acagcgccgc cgcgcgccgc tggccgttgg cgatggccgc gatctcgtcg
      961 ccgacccggc ccggcaagac cggcacgagc ttgacgctcg ccgctccgat cagggtcggg
     1021 ccgccgtggc tcgtcgagga aacgacctcg tccttcgcca gcaccttgtc attcgcaagg
     1081 atgtgcaccg gctcccaatg cacctgcggc tgcagcagca ggcggacatt gcgcagcggc
     1141 cagaccaggc ggctgtcggc atcgatcacg ggcgtgctct cggcgacgcc ctcgatcgcg
     1201 agaccgaact gctggtcacg gcccgacagg tcgagcagtg caagatgcgt gcgctcggca
     1261 tcgagcacgc cccggaagcg gttgcgcgca gcatcgtcgc gcgcagcgtc gggcggctgc
     1321 tgcggaaagc tcacggcggc gcgcaagcgc ggatgcacgg cgggcgcagc gccgaccgac
     1381 cacgccacgt cggcgtacag cgccgagtcg gccatgcgaa cgttcaggtg ctcgacgctc
     1441 cagttgctgg cgtacgggtc gggcagcatc ggctgcccga atcgcacgtc gaacaccaat
     1501 cgtgcgtggc cttcggccaa agtggccggc gcgacaccgc tgccgacgaa ggccgcgcgg
     1561 cgcgccggtt gcaggtgaag gtacaggttt tcgagggcgt agccctccac gacctccgta
     1621 tcgggatcgc gcgaggcgct tgccgctgtg cgctgcaggc cgtcgcgttc gacgatcagg
     1681 gtcgttgcag cgccagcgcc gtcgaaggcg aatggccggt cgctggcggc cagcggccgg
     1741 ttccagcggt tgtgcaagcg accgccttcc agcccgacca ggtccgggcc gccgcgccgg
     1801 ctggcgaagc gcagctccat gacgggctgc gcgccagact ccagccggct gctcgaaggg
     1861 tcccacaggg cgacgtcgat cgacaggttc gtctccgagc ggcgcagctt ccactcgaga
     1921 cctcccgcgt tcgcgctcag cacgctgtcg acggcacgac acacgccgct cgcgccggcg
     1981 atgcgcagcg cgggcccctg gcgcagcagc agcgcaatcg agccgccgtg cgcggcctcg
     2041 cccgcctgtt ccagcggcgc gttggacgag ggcagcgacc aaatcgccgc cgccacttcg
     2101 gcgctgccct cgacatagca cagcgtcgag cggttgcccg cgaggcccca ggtcgcaccg
     2161 gcggtgtcga atggaaaggc gatcgcatcg ccgccgatca caggtgcacc cgcttgcgtc
     2221 gcgaaacgca gtgtgtcacc gagccccgcc aacccgagcg cgccgctgat gaccggcgcg
     2281 acgccattgc ccagctggat ccgggacggc aggtcgatcg ccaaggcatt gccgtccgat
     2341 ccggccgctt cggcggtggg cgcgggttcg ggctcgatgc tcagcgtcca cggcgccgcg
     2401 aagggcacaa gcagagttcc atcggacagt ggcgccggca ggaccgcacc ggcgttgaag
     2461 accccgccag tgatgcgcag ggcggcgaag ccctgtgcgc cggcggccaa ccgatcagcg
     2521 cgtatccaga ccgttccggc gggaaggttc cagcgcagcg ggtcggcggc atcgggcgcg
     2581 ctggcagacg gcagcagcag gaccggttcg cggatctcga agaagggtgt ctgcaggtgc
     2641 gacacgacca cgacgaaacg cacattctcg aagaacgcg
//
LOCUS       DASRDG010000123         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_720788, whole genome shotgun sequence.
ACCESSION   DASRDG010000123 DASRDG010000000
VERSION     DASRDG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720788"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VFA35_02050"
     CDS             <1..1434
                     /locus_tag="VFA35_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZT54980.1"
                     /translation="MVGGMSKNFPAPLKCVEAVAASVRMKIDDGLRYERELFLELMLT
                     PESRALRHAFMAERATTKIPDVPADTKVRDIQKVAVIGAGTMGGGIAMNFLNAGIPVV
                     MLEMKQEALDRGVATIRKNYEAQVKKGKLKQDKLDARMALLSTTLNYADLKDADMVIE
                     AVFEDLGVKETVFKTLDEVMKPGAILASNTSTLDVNRIAAFTKRPQDVIGTHFFSPAN
                     VMKLLEVIRGEKTAKDVLATVMALGKKIKKTCVVSGVCDGFIGNRMIEEYSRQAGFLL
                     DEGATPAQVDKAMEKFGMAMGPFRMGDLAGNDIGWFIRKRRYLEKPEMRYSKTADLLC
                     EMGRYGQKTGAGWYDYAPGKRDALPAKVVTDMIEKHRADLGITARKISDDEIVQRLVY
                     ALVNEGAKIVEEGIAMRASDIDMVYLTGYGFPLFRGGPMNYADEVGLFNVVGAMKRFA
                     ANPLDDAAFWQPAPLLAKLAAEGKTFS"
     gene            1486..2661
                     /locus_tag="VFA35_02055"
     CDS             1486..2661
                     /locus_tag="VFA35_02055"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZT54981.1"
                     /translation="MTSAVIVSTARTPLAKSWKGAFNMTHGATLGGLAVKAAVERAGI
                     DGAEVDDVVIGCATPEGATGGNIARQIALRAGLPVTTSGVTVNRFCSSGLQTIAMAAQ
                     RIIAGESDVLVAGGVESISCVQNEANRHMAADPWLVEHKPEIYWNMLQTAEQVAKRYK
                     IDRERMDRYGAQSQQRATAALEAGKFADEIVACTVLAGVADPVLGLRTKEVTITNDEG
                     IRPGTTYEGIMGIKPAIPGGVIAAGNASQFSDGGGACVLMDEALASKRNLKPLGRFLG
                     FAVAGCEPDEMGIGPVFAVPKVLSKLGLRVDDIDLWELNEAFAVQVLYCAERLGIPMD
                     KLNVNGGAIAVGHPYGVSGQRLTGHALIEGKRRGAKKVVVTMCIGGGMGAAGVFEVL"
     gene            2673..>3083
                     /locus_tag="VFA35_02060"
     CDS             2673..>3083
                     /locus_tag="VFA35_02060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZT54982.1"
                     /translation="MAEPVTIDAAAFLAMGREVLAKQPFSVLLGAELVALEPGRCELG
                     LAITPQLRQQNGFAHGGVVSYLADNALTYAGGTALRVPVVTAEFKINYVRPAVGERLV
                     VRAHAAHVGKTQAVCRCDVFVVTGAVEKLCAIAQG"
BASE COUNT          565 a         1022 c         1066 g          430 t
ORIGIN      
        1 atggtcggcg ggatgtccaa gaacttcccg gcgccgctga agtgtgtgga agcggtggcc
       61 gcaagcgtcc gcatgaagat cgacgatggc ctgcggtacg agcgcgaact cttcctcgag
      121 ctgatgctca cgcccgagag cagggcgctg cgccacgcgt tcatggccga gcgcgcgacc
      181 accaagatcc ccgacgtgcc ggccgacacc aaggtgcgcg acatccaaaa ggtcgctgtg
      241 atcggcgccg gcacgatggg cggcggcatc gcgatgaact tcctgaacgc cggcatcccg
      301 gtcgtgatgc tcgagatgaa gcaggaggcg ctcgaccggg gcgtggcgac catccgcaag
      361 aactacgagg cccaggtcaa gaaaggcaag ctcaagcagg acaagctcga tgcccgcatg
      421 gccctgctga gcacgaccct gaactacgcc gacctgaaag acgccgacat ggtgatcgag
      481 gccgtgttcg aagacctggg cgtcaaggag acggtgttca agacgctcga cgaggtgatg
      541 aagcccggcg cgatcctggc cagcaacacc agcacgctcg acgtcaacag aatagccgcg
      601 ttcaccaagc gcccgcagga cgtgatcggc acgcacttct tcagcccggc caacgtgatg
      661 aagctgctcg aagtcatccg cggcgagaag accgcgaagg acgtgctcgc caccgtgatg
      721 gcgctgggca agaagatcaa gaagacctgc gtggtctcgg gcgtgtgcga cggcttcatc
      781 ggcaaccgca tgatcgagga gtactcgcgc caggccggtt tcctgctcga cgagggcgcg
      841 accccggcgc aggtcgacaa ggcgatggag aagttcggca tggcgatggg cccgttccgc
      901 atgggcgacc tggcgggcaa cgacatcggc tggttcatcc gcaagcgccg ctacctcgaa
      961 aagccggaga tgcgctacag caagacggcc gacctgctgt gcgagatggg ccgctacggc
     1021 cagaagaccg gcgccggctg gtacgactat gcgccgggca agcgcgacgc gcttcccgcc
     1081 aaggtcgtca ccgacatgat cgagaagcac cgtgccgatc tgggcatcac ggcgcgcaag
     1141 atcagcgacg acgagatcgt ccagcgcctc gtctacgcgc tcgtcaacga gggcgcgaag
     1201 atcgtcgaag agggcatcgc gatgcgcgcg tccgacatcg acatggtgta cctcaccggc
     1261 tacggcttcc cgctgttccg cggcgggccg atgaactatg cggacgaggt cggcctgttc
     1321 aacgtcgtcg gcgcgatgaa gcgcttcgcg gccaacccgc tcgacgacgc cgcgttctgg
     1381 cagcccgcgc cgctgctcgc gaagcttgcg gccgaaggca agaccttcag ctgatgcccg
     1441 cgccgcgatg aacgccagag ccaccttgat ccccaggaga cccacatgac cagcgccgtg
     1501 atcgtttcga ccgcccgcac gcccctcgcc aagagctgga agggcgcctt caacatgacg
     1561 catggcgcca cgctcggcgg cttggcggtc aaggccgcgg tcgaacgcgc cggcatcgac
     1621 ggcgccgagg tcgacgacgt cgtgatcggc tgcgccacgc ccgagggcgc gaccggcggc
     1681 aacatcgcgc gccagatcgc gctgcgcgcc ggcttgcccg tcacgaccag cggcgtgacc
     1741 gtgaaccgct tctgctcgag cggcctgcag accatcgcga tggccgcgca gcgcatcatc
     1801 gcgggcgagt cggacgtgct ggtcgccggc ggcgtcgaga gcatctcgtg cgtgcagaac
     1861 gaagcgaacc gccacatggc ggccgatccg tggctggtcg agcacaagcc cgagatctac
     1921 tggaacatgc tgcaaacggc cgagcaggtc gccaagcgct acaagatcga ccgcgagcgc
     1981 atggaccgct acggcgcgca gagccagcag cgcgcgaccg ccgcgctcga ggccggcaag
     2041 ttcgccgacg agatcgtcgc ctgcaccgtg ctggccggcg tggccgaccc ggtgctgggg
     2101 ctgcgcacga aggaagtcac gatcacgaac gacgaaggca tccgccccgg tacgacctac
     2161 gaaggcatca tgggcatcaa gccggcgatt cccggcggcg tgatcgcggc cggcaacgcg
     2221 agccagttca gcgacggcgg cggcgcctgc gtgctgatgg acgaggcgct cgcctcgaag
     2281 cgcaacctga agccgctcgg ccgcttcctc ggcttcgcgg tcgccggctg cgagcccgac
     2341 gagatgggca tcggcccggt gttcgcggtg cccaaggtgc tgagcaaact cggcctgcgt
     2401 gtcgatgaca tcgacctgtg ggagctgaac gaagccttcg cggtgcaggt gctgtactgc
     2461 gccgaacggc tgggcatccc gatggacaag ctcaacgtca acggcggtgc gatcgcggtc
     2521 ggccacccct acggcgtctc gggccagcgc ctgaccggcc acgcgctgat cgaaggcaag
     2581 cgccgcggcg ccaagaaggt ggtcgtgacc atgtgcatcg gcggcgggat gggtgcggcg
     2641 ggcgtgttcg aggtgctgtg atcgaggtcg acatggccga gccggtcacc atcgacgcgg
     2701 ccgcctttct cgcgatgggc cgcgaggttc tggcgaagca gccgttcagc gtgctgctcg
     2761 gcgccgaact cgtcgcgctc gaacccgggc gctgcgaact cgggctcgcg atcaccccac
     2821 agctcaggca gcagaacggc ttcgcgcacg gcggcgtggt cagctacctg gccgacaacg
     2881 cgctcaccta tgccggcggc acggcgcttc gcgtgccggt ggtcaccgcc gagttcaaga
     2941 tcaactatgt gcgcccggcg gtcggcgagc ggctggtggt gcgagcgcac gcggcgcatg
     3001 tcggcaagac gcaggcggtg tgccgctgcg acgtgttcgt cgtgaccggc gcggtagaga
     3061 aactctgcgc catcgcgcaa ggc
//
LOCUS       DASRDG010000124         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_377106, whole genome shotgun sequence.
ACCESSION   DASRDG010000124 DASRDG010000000
VERSION     DASRDG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377106"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="VFA35_02065"
     CDS             complement(<1..1706)
                     /locus_tag="VFA35_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZT54983.1"
                     /translation="MKSRPPVDAELSESAARTRMIGRFFFDTHAGAALASAAILALLP
                     PGLPPSVRAVLVGGTGAFALLCALAARLSGRPRFPLKTALTATAIAAIALASLTSALL
                     GEGLRGAVLAFCGPIVCVVGAITGVRRSLVVGAAALAILGALAWAEATGLVGGLAAPT
                     PLRMVLLYHGLVVVCGALAGALISRVLDHYLHTAAEREQRFRGLLRIAANWYWEQDKH
                     FRFTYVSEASTDLSGIARSDRLQRAPWEIADMGLSDEQLDAHRADLEAHRPFTDLLAR
                     RRGPAGQWRTVSISGEPKFDANGAFSGYWGVARDVTDEVLAQRAVAASETRYRELFTR
                     SPSPLFLHRRGMVFDANEAAARLFGFPNAAAMNGLQIVTLFPAGPTRERVVERMSRLD
                     AMPVGEGLPVTDFLLRAVDGRALSVQATAVRVDTASGPANLSIFFDITARKAVEGALR
                     RSEAMLSHLFATSPDCITLTEMATGRYAMVNAAFTRLTGYRADEAVGRTALELGLWHD
                     PGMLAQLLAALEHGGKVVDMPAEFVTRRGERVSTLVSAGRFAMDQRDYLVLNTRDVTV
                     SER"
     gene            complement(1810..2628)
                     /gene="pyrF"
                     /locus_tag="VFA35_02070"
     CDS             complement(1810..2628)
                     /gene="pyrF"
                     /locus_tag="VFA35_02070"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZT54984.1"
                     /translation="MNFIDALRGAETANDSMLCVGLDPDPAKFPGSWKDDPARIFDFC
                     ATIVDATRDLVIAFKPQIAYFAAQRAEDQLERLIAHIHKAAPAVPVILDAKRGDIGST
                     AEQYAREAFERYRADAVTLSPFMGFDSIEPYLAYDGKGAILLCRTSNPGGADLQAQTL
                     RNGDQLFEYIARLAAGAWNRSGQLALVVGATYPAEIERVRALAPTLPLLIPGVGAQGG
                     DAAETVRAGWRPDAPIIVNSSRAVLYASRGENFAAAARAVAQATRDALNQARPA"
     gene            complement(2663..>2867)
                     /locus_tag="VFA35_02075"
     CDS             complement(2663..>2867)
                     /locus_tag="VFA35_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZT54985.1"
                     /translation="RFFFLHTDATDYAMTALYDLLGGPNQTLSGTFATYAPVAAVPEP
                     ASVLLMAAGVLALGLTRRHRARA"
BASE COUNT          396 a         1026 c         1084 g          361 t
ORIGIN      
        1 cgctcgctca cggtcacgtc gcgcgtgttc agcaccaggt agtcgcgctg gtccatcgcg
       61 aagcggccgg ccgagaccag ggtcgagacg cgctcgccgc ggcgcgtgac gaactcggcc
      121 ggcatgtcga cgaccttgcc gccgtgttcg agcgcggcga gcagctgggc cagcatgccc
      181 gggtcgtgcc acaggccgag ctcgagcgcg gtgcggccga cggcctcgtc ggcgcggtag
      241 ccggtcaggc gcgtgaacgc cgcgttgacc atcgcgtagc ggccggtcgc catctcggtc
      301 agcgtgatgc agtcggggct ggtcgcgaac aggtgcgaca gcatcgcctc cgagcgccgc
      361 aacgcgcctt cgaccgcctt gcgggcggtg atgtcgaaga agatcgacag gttcgccggg
      421 ccgctggcgg tgtcgacccg caccgcggtc gcctgcacgc tgagcgcgcg gccgtcgacc
      481 gcgcgcagca ggaagtcggt gaccggcagg ccttcgccga ccggcatcgc gtcgagccgg
      541 ctcatgcgct cgacgacgcg ctcgcgcgtc ggcccggccg ggaacagcgt gacgatctgc
      601 agcccgttca tcgccgccgc gttcgggaag ccgaacaagc gcgccgccgc ctcgttggcg
      661 tcgaacacca tgccgcggcg gtgcaggaac agcggcgacg gcgagcgcgt gaacagctcg
      721 cggtagcgcg tctcgctggc cgcgacggcg cgctgcgcga gcacctcgtc ggtcacgtcg
      781 cgggccacgc cccagtagcc gctgaaggcg ccgttggcgt cgaacttggg ctcgccgctg
      841 atgctcacgg tgcgccattg gccggccggg ccgcggcgcc gcgcgagcag gtcggtgaac
      901 gggcggtgcg cttcgaggtc ggcgcggtgc gcgtcgagct gctcgtcgct caggcccatg
      961 tcggcgatct cccagggcgc gcgctgcagg cgatcgctgc gcgcgatgcc cgagaggtcg
     1021 gtactcgcct ccgacacgta ggtgaagcgg aagtgcttgt cctgctccca gtaccagttg
     1081 gcggcgatgc gcagcaggcc gcgaaagcgc tgctcgcgct cggccgcggt gtgcaggtag
     1141 tgatcgagca cgcgcgagat cagcgcgccg gcgagcgcgc cgcacaccac gaccagcccg
     1201 tggtacagca gcaccatgcg cagcggcgtc ggtgcggcca ggcctccgac caggccggtg
     1261 gcctcggccc aggccagcgc gcccagaatg gccagcgcgg ccgcgccgac caccaggctg
     1321 cggcgcacgc cggtgatcgc gccgacgacg cagacgatcg ggccgcagaa cgccagcacc
     1381 gcgccgcgca ggccttcgcc gagcagcgcg ctggtcaggc tggccagcgc gatcgccgcg
     1441 atcgccgtcg cggtcagcgc cgtcttcagc gggaagcgcg ggcgcccgct caggcgcgcc
     1501 gcgagcgcgc acagcagcgc gaacgcgccc gtgccgccga ccagcaccgc ccgcaccgac
     1561 ggcggcagcc cgggcggcag cagcgccagg atcgcggcgc tcgccagcgc ggcgccggcg
     1621 tgggtgtcga agaagaagcg cccgatcatc cgcgtccgcg cagcggactc ggacagttcg
     1681 gcatcgaccg gcgggcggga tttcattcgg cagggcgcaa gcgcgcgtcg gccgatgtta
     1741 cgggcccggc cgcgcgcgca gcccccgaac tgggggcgat gcaacggtca gttgtgcgga
     1801 tggggtcact caggcggggc gggcctggtt cagcgcgtcg cgcgtcgcct gcgcgacggc
     1861 gcgcgcggcc gcggcgaagt tctcgccgcg gctcgcgtac agcaccgcgc gcgacgagtt
     1921 gacgatgatc ggcgcgtccg gccgccagcc ggcgcgcacc gtctcggccg cgtcgccgcc
     1981 ctgcgcgccg acgccgggga tcagcagcgg caaggtcggc gcgagcgcgc gcacgcgctc
     2041 gatctcggcg ggataggtcg cgccgaccac cagcgcgagc tggccgctgc ggttccacgc
     2101 gcccgccgcg agccgcgcga tgtactcgaa cagctggtcg ccgttgcgca gcgtttgcgc
     2161 ctgcaggtcg gcgccgcccg ggttcgaggt gcggcacagc aggatcgcgc ccttgccgtc
     2221 ataggccaga tacggctcga tcgagtcgaa gcccatgaac ggcgacagcg tgaccgcatc
     2281 ggcgcggtag cgctcgaacg cctcgcgcgc gtactgctcg gcggtgctgc cgatgtcgcc
     2341 gcgcttcgcg tcgaggatca ccggcaccgc gggcgcggcc ttgtggatgt gcgcgatcag
     2401 gcgctcgagc tggtcctcgg cgcgctgcgc ggcgaagtag gcgatctgcg gcttgaacgc
     2461 gatcaccagg tcgcgggtcg cgtcgacgat ggtcgcgcag aagtcgaaga tgcgggccgg
     2521 atcgtccttc cacgaccccg ggaacttcgc cgggtcgggg tcgaggccga cgcacagcat
     2581 cgagtcgttc gccgtttcgg cgccgcgcag cgcatcgatg aaattcatgg gccgcgattg
     2641 taggcggccg ggcggcgcgc cctcaggcgc gggcgcggtg gcgccgcgtc agccccagcg
     2701 ccagcacgcc cgccgccatc agcagcaccg acgcgggctc cggaacggcc gcgacgggcg
     2761 cataggtcgc gaacgtcccc gacaaggtct ggttcgggcc gccgagcagg tcgtataacg
     2821 ccgtcatcgc gtagtcggtg gcgtcggtgt gcaggaagaa gaagcgc
//
LOCUS       DASRDG010000125         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_20910, whole genome shotgun sequence.
ACCESSION   DASRDG010000125 DASRDG010000000
VERSION     DASRDG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20910"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFA35_02080"
     CDS             complement(<1..309)
                     /locus_tag="VFA35_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54986.1"
                     /translation="MTAALIDELQRYVQAALESPNEMFAVVSVIIAAAFVLVSSFVKT
                     MIPLRWLAIGSNVGFVAYGALHPSYPMLLLHAALLPLNFFRLAEMKRLTRRVEAVRAE
                     G"
     gene            444..1169
                     /locus_tag="VFA35_02085"
     CDS             444..1169
                     /locus_tag="VFA35_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HZT54987.1"
                     /translation="MRTLTALLVGCCVVLPVSAADGPAITAFSSAPVGEPPAAWKFAT
                     LPNKAATKYAIVELDGAKVLKVEANESYGNLVHAVNVKASERTTLAWRWRVDKLIEDA
                     DMKSRSGEDSAAKVCVSFAFDGSKLPFGERAKLALARSSTGEDVPTETLCYIWDNKLA
                     VETSFPSVFTKRIRFIVLQTGTDKLGHWVTQRRNIAADHQRLFADESGGVVPDVIAVV
                     VGADADNTKGHGLSYVGDLTLKP"
     gene            1305..>2080
                     /locus_tag="VFA35_02090"
     CDS             1305..>2080
                     /locus_tag="VFA35_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZT54988.1"
                     /translation="MKFQPLTAALVAAGVLAVGTAGAIDLPGWLKAKPDSAPTTAQTT
                     PQPAAPAAPGVATRAPVPLLAAQGTPNYRGIVKEFGPAVVGVSVAGMHKASAEEQGLP
                     PGMENDPFFKFFRGMPGFQQRGPQGGQVPFKGMGSGFIISADGLILTNAHVVREAKDV
                     TVKLSDRREFTAKVLGSDPSTDIAVLRIAATGLPTVRIGDARSLEVGDPVLAIGAPYG
                     LEQTATQGIVSAKGRSLPGDNTVVPFIQTDAAVNPGNSGGP"
BASE COUNT          341 a          739 c          691 g          309 t
ORIGIN      
        1 gccctcggcc cgcaccgcct cgacgcgccg cgtcagccgc ttcatctcgg cgaggcggaa
       61 gaagttcagc ggcagcagcg ccgcgtgcag cagcagcatc gggtacgacg ggtgcagcgc
      121 gccgtaggcg acgaagccga cgttgctgcc gatcgcgagc cagcgcagcg ggatcatcgt
      181 cttgacgaag ctgctgacca gcacgaatgc cgcagcgatg atcaccgaca ccaccgcgaa
      241 catctcgttg ggcgattcga gtgccgcttg cacgtagcgc tggagttcgt cgatcagggc
      301 agctgtcacg gcgggttctt tcgttgagag cacggcggga ttttctcgcg ccgatggttg
      361 cgccgcatcg gtatcgtttc ggttacaacc atggtctggc gcgccgcgcc gcgccgtgcg
      421 aacgactctt ggagacgatt gccatgcgaa ccctgactgc gttgctggtg ggctgctgtg
      481 tcgtgctgcc ggtgtcggct gctgatgggc ccgcaatcac ggcgttctct tcggctcccg
      541 tgggcgagcc gccggcggcc tggaagttcg ccaccttgcc gaacaaggcg gcgaccaaat
      601 acgcgatcgt cgagctcgac ggcgcgaagg tcctgaaggt cgaagccaac gagtcctacg
      661 gcaacctggt ccacgcggtg aacgtcaaag cctcggagcg caccacgctc gcctggcgct
      721 ggcgcgtcga caagctgatc gaggacgcgg acatgaaatc gcgttccggc gaggactcgg
      781 ccgcgaaggt gtgtgtctcg ttcgctttcg acggcagcaa gctgccgttc ggcgagcgcg
      841 ccaagctggc gctggcgcgc tccagcaccg gcgaagacgt tccgaccgag acgctgtgct
      901 acatctggga caacaagctg gcggtcgaga cctccttccc cagcgtcttc accaagcgca
      961 tccgcttcat cgtgctgcaa accgggaccg acaagctcgg ccattgggtc acgcagcgcc
     1021 gcaacatcgc ggccgatcac cagcgcctgt tcgccgatga atccggcggc gtggtgcccg
     1081 acgtgatcgc ggtcgtcgtc ggcgccgatg ccgacaacac gaaggggcac ggcctgtcgt
     1141 atgtcggcga cctgacgctg aagccgtgaa gcgcgcgcca ccggccgccc ggcgctggcc
     1201 cctaaccctg gcctaaccgg gcgctaaccg agccctaacc gaacgctcct atctttgccc
     1261 gcatgcggaa tcatccgctt tcgcctcggg cacaaggagc acccatgaaa ttccaacccc
     1321 tcactgcggc gctcgtcgcc gcgggcgtgc tggccgtcgg caccgccggc gcgatcgacc
     1381 tgccgggctg gctgaaagcc aagccggact cggcaccgac caccgcgcag accaccccac
     1441 agcccgccgc cccggctgcg cccggcgtcg ccacgcgggc gccggtgccg ctgctcgcgg
     1501 cgcagggcac gccgaactac cgcggcatcg tcaaggagtt cggccccgcg gtcgtcggcg
     1561 tcagcgtggc tggcatgcac aaggcctcgg ccgaagagca gggcctgccg ccgggcatgg
     1621 agaacgaccc gttcttcaag ttcttccgcg gcatgccggg ctttcagcag cgcggcccgc
     1681 agggcggcca ggtgccgttc aagggcatgg gctcgggctt catcatcagc gccgacggcc
     1741 tgatcctgac caacgcccac gtcgtgcgcg aggcgaagga cgtgaccgtc aagctcagcg
     1801 accggcgcga gttcacggcc aaggtgctcg gctccgaccc gagcaccgac atcgcggtgc
     1861 tgcgcatcgc cgcgaccggc ctgccgaccg tgcgcatcgg cgacgcgcgc agcctcgagg
     1921 tcggcgaccc ggtgctcgcg atcggcgcac cgtacgggct ggagcagacc gccacccaag
     1981 gcatcgtcag cgccaagggc cgctcgctgc cgggcgacaa cacggtcgtg ccgttcatcc
     2041 agaccgacgc cgcggtgaac cccggcaact cgggcggacc
//
LOCUS       DASRDG010000126         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_21276, whole genome shotgun sequence.
ACCESSION   DASRDG010000126 DASRDG010000000
VERSION     DASRDG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21276"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFA35_02095"
     CDS             complement(<1..423)
                     /locus_tag="VFA35_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54989.1"
                     /translation="MNWADETLMAFVDGELDAARRADLEGALAGDAALLARVAALRAQ
                     RQRVSAAFAGVLDEPMPDRLASLLADAPAAHTPAVVDLRAARSARAPRRALPGWAQWG
                     GMAASVVLGLVLGLQLAGHGGDGSLGVRDGRLVVGGAVD"
     gene            complement(420..926)
                     /locus_tag="VFA35_02100"
     CDS             complement(420..926)
                     /locus_tag="VFA35_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZT54990.1"
                     /translation="MVESSQNVREQLAALLPRLRRFGRTLARNREDADDLVQMALERA
                     LARSDQWQAGTRLDSWMFRIMQNAWIDETRARERRGQTFVAEELGEHVGVSTTDAQID
                     AIAVRKAVAQLNDDQRAAVGLVLVEGLPYKEAAEVLGVPIGTLTSRLARARESLQALL
                     DAPRGAGA"
     gene            853..2307
                     /locus_tag="VFA35_02105"
     CDS             853..2307
                     /locus_tag="VFA35_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZT54991.1"
                     /translation="MRPKRRRRGSSAASCSRTFCDDSTIDPRYNGWWVDFLPCPVRYP
                     RMHMTRAHPPCAHRPWRGPCAIALAALAVAPLAHAQLALPRVTLPALPRLPSTNLPER
                     GLNVAPLQSLRTNTIRELLRDHADVLEPDPSGEPIRRHELLLVSPPRTTLDAALVQGF
                     VLLREQSLPALDLTQIVLLAPPGVGTAEALARLRAIDPAVEADFNHVYTRSGELAPAP
                     DATAAMASASTSRRVGLVDSGIDRRHASLRNAHVQAWGCDGNQMPSPHGTAIASLLVG
                     RDGAFSGAVPGVALYAADIYCGQAAGGAVESLALALAWMAREKVAVINVSLVGPANRV
                     LQRAVTALTERGHLIVAAVGNDGPAAPPLYPASYPGVVGVTGITPSRRVLPEAAQGPQ
                     VMFSAPGSELAVARPGGGYVVARGSSFAAPLVAGLLAEALQVPDRLAAAAALARVAET
                     AQDLGAPGRDPVYGVGLVAEHARIAPERVQAAAR"
BASE COUNT          320 a          922 c          853 g          370 t
ORIGIN      
        1 atcgaccgcg ccgccgacga caagacggcc gtcgcgcaca ccgagcgagc cgtcaccgcc
       61 gtggccggca agctgcaggc cgagcaccag acccagtacc acgctggcgg ccatgccgcc
      121 ccattgcgcc cagccgggca gcgcgcggcg cggcgcccgc gcgctgcggg cggcacgcaa
      181 gtccaccacg gccggcgtgt gcgcggcggg tgcatcggcc agcaggctcg ccaggcgatc
      241 gggcatgggc tcgtcgagca cgccggcgaa ggccgccgaa acacgctggc gctgcgcccg
      301 cagcgccgcc acacgagcca gcagtgccgc atcaccggcc agcgcgcctt cgagatcggc
      361 acgccgcgcc gcgtcgagct cgccgtcgac gaaggccatc agcgtttcat cggcccagtt
      421 catgcgcccg ctccccgggg cgcgtcgagc agcgcctgca gcgattcgcg cgcccgcgcc
      481 agtcggctgg tcagggtgcc gatcggcacg cccagcacct cggccgcttc cttgtacggc
      541 aggccttcga ccagcaccaa gcccacggcg gcgcgctggt catcgttgag ttgcgcgacc
      601 gccttgcgca ccgcgatggc gtcgatctgc gcgtcggtgg tcgacacgcc cacgtgctcg
      661 cccagctcct cggccacgaa ggtctggccg cggcgctcgc gggcgcgtgt ctcgtcgatc
      721 caggcgttct gcatgattcg aaacatccaa ctgtccagcc gcgtgcccgc ttgccattga
      781 tcgctgcgcg ccagcgcgcg ctcgagcgcc atctgcacga ggtcgtcggc gtcttcgcgg
      841 ttgcgggcca gcgtgcgtcc gaagcgccgc aggcgcggca gcagcgccgc cagctgttcc
      901 cgcacgttct gtgacgattc gaccatcgat cctcggtaca acgggtggtg ggtggatttt
      961 cttccatgcc cggttcgtta tcctcgcatg cacatgacgc gtgcccaccc accctgcgca
     1021 catcggccct ggcgcggacc ctgcgcgatc gccctggcgg ccctcgccgt cgccccgctg
     1081 gcacacgcgc aactggctct gcctcgcgtc acgctgcccg cgctcccgcg ccttcccagc
     1141 accaacctgc ctgaacgggg tctgaacgtg gcgccgcttc agtcgctgcg cacgaacacg
     1201 atccgtgagc tgctgcgtga tcacgccgac gttctggagc ccgatccctc cggcgaaccg
     1261 atccggcgtc acgaactgct gctggtgtcg ccgccgcgca cgaccctcga cgccgccctg
     1321 gtgcagggct tcgtgctgct gcgcgagcaa tcgctgccgg cactggacct gacgcagatc
     1381 gtcttgctgg cgccgccggg cgtgggcacg gccgaggccc tggcgaggct gcgcgcgatc
     1441 gatccggcgg tcgaggccga cttcaaccac gtctacacgc gcagcggcga gctggcgccg
     1501 gcacccgacg cgacggccgc catggcttct gcgtcgacat cgcgccgggt cggcctggtc
     1561 gacagcggca tcgaccggcg gcatgcgtcg ctgcgcaacg cccatgtcca ggcttggggt
     1621 tgcgacggca accagatgcc gtcgccgcat ggcaccgcga tcgcgtcgct gctggtggga
     1681 cgcgacggcg cgttcagcgg tgctgtgccc ggcgtcgcgc tgtacgctgc cgacatctat
     1741 tgtggccaag ccgccggggg cgccgtcgaa agcctcgcgc tggcgctcgc gtggatggcg
     1801 cgcgagaaag tcgcagtgat caacgtcagc ctggtcgggc cggccaaccg cgtgctccag
     1861 cgtgcggtga cggcgctgac cgaacgcggc cacctcatcg tggccgcggt cggcaacgat
     1921 ggcccggccg cgccgccgct gtacccagcg tcgtatcccg gcgtggtcgg cgtgacgggc
     1981 atcacgccgt cgcgccgcgt cctgcccgaa gctgcgcagg gcccgcaggt gatgttctcg
     2041 gcgcccggct ccgagctggc ggtggcacgg ccgggcggcg gctacgtcgt ggcgcgcggc
     2101 agttcgtttg cggcgccgtt ggtggctggc ctgctcgcag aggcactaca ggttcccgac
     2161 cggcttgctg ccgcggcggc gctggctcgc gtggccgaga cggcacagga cctcggcgcg
     2221 cccggccgcg acccggtcta cggcgtgggt ctggtggccg agcacgcgcg catcgcgccg
     2281 gagcgcgtgc aggcggcggc gcgctgacgc gagaccaggc tgcgcctggt tacaacgatt
     2341 cttgcgcgac cacggatcga accgcgcccg aggcacgttg tgctctgtgc aggggccgga
     2401 gggtccggtc cttcaaaccg ctcaatggag ttctctcatc atggtcaatc attccactct
     2461 gttcc
//
LOCUS       DASRDG010000127         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_377654, whole genome shotgun sequence.
ACCESSION   DASRDG010000127 DASRDG010000000
VERSION     DASRDG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377654"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFA35_02110"
     CDS             complement(<1..228)
                     /locus_tag="VFA35_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54992.1"
                     /translation="MWILTEAPRGSNFYEAASTTGNKALISDTCDTVIYARSQGADGF
                     RMVAQRGRETFFIGPTPVRGVAVDINAQLLEV"
     gene            277..900
                     /locus_tag="VFA35_02115"
     CDS             277..900
                     /locus_tag="VFA35_02115"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZT54993.1"
                     /translation="MNELQALLNDTPAAALILAAMVAASLFGLYRSPALIERNLFRPY
                     WLVPQHQWHTLITSGFLHADMTHLLFNAFTFWAFGFGLERTIGTPAFVALYFVGLLVS
                     DAGTWLQHRHDPEYRSLGASGAILAVLFASIVYHPSSSIFILPIPVPIPAPLFALGYL
                     GYSWWAARHTRGNINHDAHLAGALTGIAFVALSDWPTLTRALRSFFA"
     gene            complement(902..>2183)
                     /locus_tag="VFA35_02120"
     CDS             complement(902..>2183)
                     /locus_tag="VFA35_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZT54994.1"
                     /translation="FGKEAARCAVMAYDYTVLAGTQGWRNHHKQDRMPALAHQQALPM
                     VLFAEGGGGRPGDIDMPVVAGLNNHTFSQFAALSGKVPVLGIVHGRCFAGNAALLGCV
                     DTIIATRGSNIGMSGPAMIEGGGLGRYTPEQIGPSDVQSASGVIDVLVDDEAQAVRAG
                     RQYLGYFQGVASAWQCADQRLLRHAVPENRLRVYDVRQAMRTLVDSDTLLELRAGFGA
                     GIVTALARIEGRPVGLMANNPKHLGGAIDPEAADKAARFMQLCDAHGLPIVSLVDTPG
                     FMVGPDIEARAQVRHVCRMFLVAAHLTVPFFSVVLRKGYGLGAQAMTAGGFDAPVFNI
                     SWPTGEFGGMGLEGAVRLGYRKELEAVAEGAARDARFRELVAQQYARGSAINMAATLE
                     IDAVIDPAETRSWLVRGLRAAPARRGKRGYVDAW"
BASE COUNT          293 a          795 c          751 g          344 t
ORIGIN      
        1 cacctcgagc agctgggcgt tgatgtcgac cgcgacgccg cgcaccggtg tcggcccgat
       61 gaagaaggtc tcgcgaccgc gctgcgcgac catgcgaaag ccgtcggcgc cctgcgaacg
      121 cgcgtagatc acggtgtcgc aggtgtcgga gatcaaggcc ttgttgccgg tcgtcgatgc
      181 ggcttcgtag aaattgctgc ctcgcggcgc ctcggtgagt atccacatgg tcgggttctc
      241 gtgcgctcgt gagcgggaac gtctaatgtg ccgcagatga acgaactcca agcgctgctg
      301 aacgataccc ctgccgcggc cctgatcctc gcggcgatgg tggccgcgag cctgttcggg
      361 ctttatcggt cgccggcgct gatcgagcgc aacctgttcc gcccgtactg gctggtgccg
      421 cagcaccagt ggcacacgct gatcaccagc ggcttcctgc acgcggacat gacgcacctg
      481 ctgttcaacg cgttcacgtt ctgggccttc ggcttcgggc tcgagcgcac gatcggcacg
      541 ccggcgttcg tcgcgctgta cttcgtcggg ctgctcgtca gcgacgccgg cacctggctg
      601 cagcatcgcc acgatcccga ataccgcagc ctgggcgcgt cgggcgcgat cctcgcggtg
      661 ctgttcgcgt ccatcgtcta ccacccgtcg agctcgatct tcatcctgcc gatcccggtg
      721 ccgatcccgg cgccgctgtt cgcgctcggc tacctcggct acagctggtg ggcggcgcgg
      781 cacacgcgcg gcaacatcaa ccacgacgcg cacctcgcgg gcgcgctgac cggcatcgcg
      841 ttcgtcgcac tgagcgactg gcccacgctg acgcgggcgc tgcggtcctt cttcgcttag
      901 ctcaccacgc gtcgacatac ccgcgcttgc cgcgccgcgc cggcgccgcg cgcaggccgc
      961 gcaccagcca cgagcgcgtc tcggcggggt cgatcaccgc gtcgatctcg agcgtcgccg
     1021 ccatgttgat cgcgctgccc ctggcgtact gctgcgcgac cagctcgcgg aaccgggcgt
     1081 cgcgcgcggc gccttcggcg acggcctcga gctccttgcg gtagccgagc ctgaccgcgc
     1141 cttcgagccc catgccgccg aactcgccgg tcggccacga gatgttgaac accggcgcgt
     1201 cgaaaccgcc ggcggtcatc gcctgcgcgc ccaggccgta gcccttgcgc agcacgaccg
     1261 agaagaacgg caccgtgagg tgcgccgcga ccaggaacat gcggcagacg tggcgcacct
     1321 gcgcgcgcgc ctcgatgtcg gggccgacca tgaaaccggg cgtgtcgacg agcgagacga
     1381 tgggcagccc gtgcgcgtcg cacagctgca tgaagcgcgc cgccttgtcg gcggcctcgg
     1441 ggtcgatcgc gccgccgagg tgcttcgggt tgttggccat cagcccgacc gggcggccct
     1501 cgatgcgcgc cagcgccgtg acgatgccgg cgccgaagcc ggcgcgcagc tcgagcagcg
     1561 tgtcgctgtc gacgagcgtg cgcatcgcct ggcgcacgtc gtagacgcgc agccggttct
     1621 cgggcacggc gtggcgcagc agccgctggt cggcgcactg ccacgcgctc gcgacgccct
     1681 ggaagtagcc caggtactgc cggccggcgc gcaccgcctg cgcctcgtcg tcgaccagca
     1741 cgtcgatcac gccgctggcc gactgcacgt cgctcgggcc gatctgctcg ggcgtgtagc
     1801 gcccgagacc gccgccttcg atcatcgccg gcccgctcat gccgatgttg ctgccgcgcg
     1861 tggcgatgat ggtgtcgacg cagccgagca gcgcggcgtt gccggcgaag cagcgcccgt
     1921 ggacgatgcc gagcaccggc accttgcccg acagcgcggc gaactggctg aaggtgtggt
     1981 tgttgaggcc ggcgaccacc ggcatgtcga tgtcgcccgg gcgcccgccg ccgccctcgg
     2041 cgaacagcac catcggcagc gcctgctggt gcgccagtgc gggcatgcgg tcctgcttgt
     2101 ggtggttgcg ccagccctgc gtgccggcga gcacggtgta gtcgtaggcc atcacggcgc
     2161 agcgcgccgc ttccttgccg aac
//
LOCUS       DASRDG010000128         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_822194, whole genome shotgun sequence.
ACCESSION   DASRDG010000128 DASRDG010000000
VERSION     DASRDG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822194"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VFA35_02125"
     CDS             <1..1470
                     /locus_tag="VFA35_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZT54995.1"
                     /translation="LSLERDLFARLREEAESVAIRVFAENLRDLLLAAPAGPRVVMGL
                     DPGIRTGCKVAVVDATGKVLATDTVYPHEPRRDWEGSLHSLAKLCATHGVNLIAIGNG
                     TASRETDKLAGDLIKRLQRNAPDTKIEKIVVSEAGASVYSASEYASKELPELDVSLRG
                     AVSIARRLQDPLAELVKIDPKSIGVGQYQHDVNQSELAKSLSTVIEDCVNSVGVDLNT
                     ASTPLLARVSGLSSAVAASIVRWRDANGAFRSRKQLLDVSGLGAKTFEQSAGFLRIRG
                     GDNALDASGVHPETYPLVEKMLAAVNKPAAEVMGRSDVIRALKPEAFADEKFGAITVK
                     DVLAELEKPGRDPRPDFKVARLTDGIEDIKDLAAGMTLEGTISNVAQFGAFVDLGVHQ
                     DGLIHVSQLANKFVNDAREVVKTGDIVKVRVLEVDLARKRISLTMKLDAGPVPQGERG
                     NAYRAPARGERIVASHRAAQSQAGGSAMADAFARLQAKR"
     gene            1510..1998
                     /locus_tag="VFA35_02130"
     CDS             1510..1998
                     /locus_tag="VFA35_02130"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZT54996.1"
                     /translation="MNLPKHELMMTVLMTPDMANFSGNVHGGTVLKLLDQVAYACASR
                     YAGRYVVTLSVDQVMFREPIHVGELVTFLACVNFTGTTSMEIGIKVVTENIVTQTVRH
                     ANSCFFTMIAMGDDKKPVAVPPLELRDDDQRRRDAAARMRRQLRQEFARRLEKEKVGN
                     AG"
BASE COUNT          409 a          731 c          750 g          319 t
ORIGIN      
        1 ctgagcctgg agcgcgacct gttcgcgcgc ctgcgcgaag aggcggaaag cgtcgcgatc
       61 agggtcttcg ccgagaacct gcgcgacctg ctgctggccg cgccggccgg cccgcgcgtc
      121 gtgatgggcc tggaccccgg catccgcacc ggctgcaagg tggcggtggt cgacgcgacc
      181 ggcaaggtgc tggcgaccga cacggtctat ccgcacgagc cgcgccgcga ctgggaaggc
      241 tcgctccatt cgctcgcgaa gctctgtgcg acccatggtg tgaacctgat cgcgatcggc
      301 aacggcaccg cgagccgcga gaccgacaag ctcgccggcg acctgatcaa gcgcctccag
      361 cgaaacgcgc cggacacgaa gatcgagaag atcgtcgtca gcgaggccgg cgcgtcggtc
      421 tactcggcca gcgagtacgc gagcaaggag ctgcccgagc tcgacgtgag cctgcgcggc
      481 gccgtcagca tcgcacggcg cctgcaggac ccgcttgccg agctcgtcaa gatcgacccg
      541 aagagcatcg gcgtcggcca gtaccagcac gacgtcaatc agagcgagct cgcgaagagc
      601 ctgagcacgg tgatcgagga ctgcgtcaat tcggtcggcg tcgacctgaa caccgcgtcg
      661 acgccgctgc tggcgcgcgt ctcgggcctg agcagcgccg tcgccgcgag catcgtgcgc
      721 tggcgcgatg cgaacggcgc gttccgcagc cgcaagcagt tgctggacgt gagcggtctc
      781 ggggccaaga ccttcgagca gagcgccggc ttcctgcgca tccgcggcgg cgacaacgcg
      841 ctcgacgcgt cgggcgtgca ccccgagacc tacccgctgg tcgagaagat gctcgccgcg
      901 gtcaacaagc cggcggccga ggtgatgggc cgcagcgacg tgatccgcgc gttgaaaccg
      961 gaggcgttcg ccgacgagaa gttcggcgcg atcaccgtga aggacgtgct ggccgaactc
     1021 gagaagccag gccgcgaccc gcgcccggac ttcaaggtcg cgcgcctgac cgacggcatc
     1081 gaggacatca aggatctggc cgcgggcatg acgctcgaag gcacgatcag caacgtcgcg
     1141 cagttcggcg ccttcgtcga cctcggcgtg caccaggacg gcctgatcca cgtcagccag
     1201 ctcgccaaca agttcgtgaa cgacgcgcgc gaggtcgtca agaccggcga catcgtcaag
     1261 gtcagggtgc tcgaggtcga cctggcgcgc aagcgcatct cgttgacgat gaagctcgac
     1321 gccgggccgg tgccgcaagg cgagcgcggc aatgcgtacc gtgcgccggc gcgcggcgaa
     1381 cgcatcgtcg cgtcgcaccg cgcagcacaa tcgcaggcgg gcggctcggc gatggccgat
     1441 gcgtttgcgc ggctgcaggc caagcggtga caatgcgctg agaagatccg aagcgacgag
     1501 ggcaccacga tgaatctgcc gaagcacgaa ctgatgatga ccgtgctgat gacgcccgac
     1561 atggcgaact tcagcggcaa cgtgcacggc ggcacggtgc tgaagctgct cgaccaagtc
     1621 gcctacgcgt gcgcgagccg ctacgccggc cgctacgtgg tgacgctgtc ggtcgatcag
     1681 gtgatgtttc gcgagccgat ccatgtcggc gagctcgtga cctttctcgc ctgcgtgaac
     1741 ttcaccggca cgacctcgat ggagatcggc atcaaggtcg tcaccgagaa catcgtgacg
     1801 cagacggtgc ggcatgccaa cagctgcttc ttcacgatga tcgcgatggg cgacgacaag
     1861 aagccggtgg ccgtgccgcc gctcgaactg cgcgacgacg accagcgccg gcgcgacgcc
     1921 gcggcgcgca tgcggcgcca gctacgccag gagttcgcgc ggcgcctgga gaaagagaag
     1981 gtcggcaacg ccggctagaa tcgccgcttc agcaacggac aaggacgaga aaggcaatga
     2041 tggttatgac accgcgaact accaccttct tcacgaaggt ctagtcggcc gcgcgtctcg
     2101 acctgcattc ccattccctt tctcgaacaa ggcgcggctc agtccgcgcc ttttttcatt
     2161 tggagttctc gatggtctcg attcacttgc ccgacggctc gacgcgtga
//
LOCUS       DASRDG010000129         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1529356, whole genome shotgun sequence.
ACCESSION   DASRDG010000129 DASRDG010000000
VERSION     DASRDG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529356"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFA35_02135"
     CDS             complement(<1..514)
                     /locus_tag="VFA35_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54997.1"
                     /translation="MELADDLVEQFADRDALYRDIDAVLFGELPVEIPEAYRKTAIEV
                     RSPLAMHIATTVTAALSVNPMTTVFKPIGFGDTYQQNSTLREHFFEASWIRQEQEARR
                     QLLRLFMWSLAVKGEGIVKTLERTRAAWNEYDEKSKDLEKSLEKDPELDQHAKDLMYD
                     HETEDYKLALP"
     gene            complement(592..1296)
                     /locus_tag="VFA35_02140"
     CDS             complement(592..1296)
                     /locus_tag="VFA35_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54998.1"
                     /translation="MTFNPPTFLPPVLAVGQSNSSNGRQQLGSGLVTHRGIIDSNSLR
                     VQGWLNPQSGRGTELGFQSTAAYGYLQVIDRDSQTWLDLQVSARNITLTATSGKVSLP
                     AGTAQQLIGSYRAIAGWNIPATSAWYESVAQVNATTTGGVLRLEACGTVTHSVATAIM
                     YIGLMIDGNPGYADSQTAVQPSLNGQLMPYSIIYYLGGLAAGAHRFSIMLYSPNMGAG
                     LWSGAFQNLYVTEQRA"
     gene            complement(1293..>2151)
                     /locus_tag="VFA35_02145"
     CDS             complement(1293..>2151)
                     /locus_tag="VFA35_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT54999.1"
                     /translation="YQQLLADLNPREPSFWLYDRERDGKTTFLFARHADNPTITPERL
                     APLEALTGYLRDRLLLGLRVAAEGMYFTEFDPRLHLVEAFDPPEDWIRWVSVDWGFAH
                     PWVALWFARERPGGHIYVYREISAAGIRDEQQAKLIIDRSGDERIAQVVLDPSMFNSR
                     TESMRPSIAQVYAGSGVKSIVPGFNSRTQGWAVVRRALAHGEQVALQRRVELEALPRL
                     QIMHERCPNLVRNLPAMVMDPLDPEDVADKIGGTRTPDDEVDALRYGLCAEAQTGVIE
                     EPVGKVMFG"
BASE COUNT          358 a          698 c          680 g          415 t
ORIGIN      
        1 acggcagcgc gagcttgtag tcctcggtct cgtggtcgta catcaagtcc ttggcgtgct
       61 ggtcaagctc agggtctttt tcgaggcttt tttcgaggtc cttggacttt tcgtcgtact
      121 cattccaggc cgcgcgcgtg cgctcgagcg tcttgacgat gccctcgcct ttgaccgcca
      181 ggctccacat gaacagccgc agcaactgac gcctggcttc ctgctcctgg cggatccagc
      241 tcgcctcaaa gaagtgctcg cgcagcgtgg agttctgctg gtaggtgtcg ccgaagccga
      301 tcggtttgaa cacggtcgtc atcgggttga cgctgagcgc cgcggtgacc gtcgtagcaa
      361 tgtgcatcgc cagcggactt ctcacctcga tggccgtctt acggtacgcc tcggggattt
      421 ccaccggcag ctcgccgaac agcaccgcgt cgatgtccct gtacagcgcg tcgcgatcgg
      481 cgaactgctc gaccaggtcg tcggcgagtt ccatggtggc gcgctgcatg gcgtcctcgt
      541 cgctgcttgt cgagttcttg aaccagcccg ccggcggcgc cgaagacatg ctcatgcgcg
      601 ttgctccgtg acgtagaggt tctgaaaggc accgctccac aggccggcgc ccatgttggg
      661 gctgtacagc atgatcgaga agcgatgcgc gccagctgcc aggccgccca ggtagtagat
      721 gatcgaatac ggcatgagct gaccgttcaa tgacggctgc acagcagtct gtgagtcggc
      781 atagccggga ttgccgtcga tcatcagccc gatgtacatg atcgcggtcg ccacgctgtg
      841 cgtgacggtg ccgcacgcct ccagacgcag cacgccaccc gtggtggtgg cgttgacctg
      901 ggcaaccgac tcgtaccagg cgcttgtcgc cgggatattc cagccagcta tcgcgcgata
      961 gctgccaatc aactgctgcg ccgtacccgc cggcaacgac accttgccgc tagttgcggt
     1021 cagggtgatg ttcctggcgc ttacctgcag gtccagccac gtctggctgt cgcggtcgat
     1081 gacttgcagg tagccgtagg ccgccgttga ttggaagccg agttcggtgc cacgcccgct
     1141 ttgcgggttc agccagccct gcaccctgag actgttggag tcgatgatgc cgcgatgcgt
     1201 gaccaacccc gaacccagct gctggcgccc gttgctggag ttgctctggc ccacggccag
     1261 caccggtggc agaaacgtgg gcgggttgaa tgtcacccga acatcacctt gccgaccggc
     1321 tcttcgatga caccggtctg agcttcagcg cacaacccat agcgcaatgc atccacctcg
     1381 tcatcgggcg tgcgcgtgcc gccgatcttg tccgcgacat cctccggatc aagtgggtcc
     1441 atgaccatcg ccggcagatt gcgcaccagg tttgggcagc gctcgtgcat gatttgcagg
     1501 cgcggcagag cctcgagttc cacgcgtcgc tgcaatgcga cctgctcacc atgcgcgagt
     1561 gccctgcgca caactgccca gccctgcgta cgactgttga acccgggcac gatgctcttg
     1621 acgccgctgc cggcgtacac ctgggcgatg ctcggacgca tcgactcggt gcgtgagttg
     1681 aacatgctcg gatcgagcac cacctgggcg atgcgctcgt cgccagagcg atcaatgatc
     1741 agcttggcct gctgctcgtc gcggatgccg gcggcggaga tttcgcggta cacgtagatg
     1801 tggccgcctg gtcgctcgcg cgcaaaccac agcgcgaccc agggatgtgc aaagccccag
     1861 tcgaccgaca cccacctgat ccagtcctcg ggcggatcga acgcctccac caggtgcagc
     1921 cgcgggtcga actcggtgaa gtacatgccc tccgcggcca cccgtagccc cagcagcagg
     1981 cggtcgcgga ggtagccagt gagggcctcg agcggagcca gccgctctgg cgtgatggtc
     2041 gggttgtcgg catgccgcgc aaacagaaag gtcgtcttgc cgtcgcgctc gcggtcatac
     2101 agccagaagc tgggctcacg tgggttcagg tcggccagga gctgctggta c
//
LOCUS       DASRDG010000130         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_477677, whole genome shotgun sequence.
ACCESSION   DASRDG010000130 DASRDG010000000
VERSION     DASRDG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477677"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFA35_02150"
     CDS             complement(<1..315)
                     /locus_tag="VFA35_02150"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZT55000.1"
                     /translation="MLHYPAFNPVALQIGPVAIHWYGLTYLVAFGLFIWLGSIRVRLV
                     QFASRGWSRRDIEDLLFYGVLGVVIGGRIGYVLFYKPGYYAAHPFEIVAVWKGGMSFH
                     GGM"
     gene            complement(336..1286)
                     /locus_tag="VFA35_02155"
     CDS             complement(336..1286)
                     /locus_tag="VFA35_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZT55001.1"
                     /translation="MNVADPSIELRSWRQFLMLAQTLHFGRAAELLHITQPPLTQAIQ
                     QLERRLGTRLFERTRRSVALTPAGAALVEPVRQLLQQAAALQARARDAAAGEAGRVRL
                     GFISTVGFGPLPLWLRAFREQQPGIAIELIEATSDVQLKAFERGEIDVGFMLHAPGLA
                     PAAPVKLQSLSLGIEPLVLALPDAAPWAKARRLQPARLLAQPLVIFPRSATPSLYDAV
                     IAFYHRHGATPVIAQQATQMQTIVNLVSAGLGIALVPEAVTQLQRPGVVYRPLPGALV
                     DLAPHCETSLLWPVDAAPAVTRFVEFVRALAPQPLGYRSR"
     gene            1375..>2599
                     /locus_tag="VFA35_02160"
     CDS             1375..>2599
                     /locus_tag="VFA35_02160"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZT55002.1"
                     /translation="MTDPADKINRRSAHITEGVARAPNRSMYYAMGYEEGDFKKPMIG
                     VANGHSTITPCNSGLQKLADAAVAGIEAAGGNAQIFGTPTISDGMAMGTEGMKYSLVS
                     REVIADCIETCVGGQWMDGVLVVGGCDKNMPGGMMGMLRANVPAIYVYGGTILPGRYK
                     GQDLNIVSVFEAVGQFSAGNMSEEDFCQIERRAIPGTGSCGGMYTANTMSSSFEALGM
                     SLPFSSTMANPHDEIVAHTREAARVLVEAVTNDLKPRDIVTRKSIENAVAVIMATGGS
                     TNAVLHFLAIAHAAEVEWTIDDFERVRQGVPVLCDLKPSGQYLAVDLHRAGGIPQVMK
                     TLLAAGLLHGDCITITGKTVAENLAAIPDAPRTDQDVIRPIGRPMYEHGHLAILKGNL
                     SPEGSVAKITGLKNPV"
BASE COUNT          473 a          898 c          891 g          337 t
ORIGIN      
        1 catgccgccg tggaacgaca tgccgccctt ccagaccgcg acgatctcga acggatgcgc
       61 cgcgtagtag cccggcttgt agaacagcac atagccgatg cgcccgccga tcacgacgcc
      121 gagcacgccg tagaacaaca ggtcttcgat gtcgcggcgc gaccagccgc gcgaggcgaa
      181 ctgcacgagc ctgacgcgga tcgatccgag ccagatgaac agcccgaacg cgaccaggta
      241 ggtcaggccg taccaatgga tcgcgaccgg gccgatctgc agcgcgacgg ggttgaacgc
      301 gggatagtga agcatgtcag cggagccacc ggaaatcagc gcgaacgata ccccagcggc
      361 tgcggcgcaa gcgcccgcac gaactcgacg aagcgcgtga ccgcgggcgc tgcgtccacc
      421 ggccagagca ggctggtctc gcagtggggc gcgagatcga ccagcgcgcc gggcaagggg
      481 cgatagacga cgcccggccg ctgcagttgc gtgacggcct cgggcacgag cgcgatgccc
      541 aggccggccg agacgaggtt gacgatggtc tgcatctgcg tcgcctgctg cgcgatcacc
      601 ggcgtggcgc cgtggcggtg gtagaacgcg atcaccgcgt cgtacaacga cggcgtcgcg
      661 ctgcgcggga agatcacgag cggctgcgcc agcagcctgg ccggctgcaa gcggcgcgcc
      721 ttggcccacg gcgccgcgtc gggcaaggcc agcaccagcg gttcgatgcc gagcgacagg
      781 ctttgcagct tcaccggcgc ggccggcgcc agccccggcg cgtgcagcat gaagccgaca
      841 tcgatctcgc cgcgctcgaa ggccttcagc tgcacgtcgc tggtcgcctc gatcagctcg
      901 atcgcgatgc cgggctgctg ctcgcggaac gcgcgcagcc acagcggcag cgggccgaag
      961 ccgacggtcg agatgaagcc caggcgcacg cgcccggcct cgcccgccgc cgcgtcgcgc
     1021 gctcgcgcct gcagggccgc ggcctgctgc agcaactgcc gcaccggctc gaccagcgcg
     1081 gcgccggccg gcgtcagcgc gacactgcgc cgcgtgcgct cgaacaagcg cgtgcccagg
     1141 cgccgttcga gctgctggat cgcctgcgtc agcggcggct gggtgatgtg cagcagctcg
     1201 gcggcgcgcc cgaagtgcag cgtctgcgca agcatcagaa actggcgcca gctgcgcagc
     1261 tcgatcgacg ggtcggcgac attcattcgc caagcatatc aatgcgccac ttcatatcta
     1321 ttggacgcag gctttccatc gcccgatact ctgcaacctc gaaggagaca tgcgatgacc
     1381 gatcccgccg acaaaatcaa ccgccgttcc gcccacatca ccgagggcgt ggcacgcgcg
     1441 ccgaaccgct cgatgtacta cgcgatgggc tacgaggaag gcgacttcaa gaagccgatg
     1501 atcggcgtcg ccaacggcca ctcgacgatc acgccgtgca actccgggct gcaaaagctc
     1561 gccgacgcgg cggtcgccgg catcgaggcc gccggcggca acgcgcagat cttcggcacg
     1621 ccgacgatca gcgacggcat ggcgatgggc accgagggca tgaagtacag cctggtctcg
     1681 cgcgaggtga tcgccgactg catcgagacc tgtgtgggcg gccagtggat ggacggcgtg
     1741 ctggtggtcg gcggctgcga caagaacatg cccggcggca tgatgggcat gctgcgcgcc
     1801 aacgtgccgg cgatctacgt ctacggcggc acgatcctgc cgggccgcta caagggccag
     1861 gacctgaaca tcgtcagcgt cttcgaggcg gtcggccagt tcagcgccgg caacatgagc
     1921 gaagaagact tctgccagat cgagcgccgc gcgatccccg gcaccggctc gtgcggcggc
     1981 atgtacaccg cgaacacgat gagctcgtcg ttcgaggcgc tgggcatgag cctgccgttc
     2041 tcgagcacga tggcgaaccc gcacgacgag atcgtcgcgc acacgcgcga agcggcgcgc
     2101 gtgctggtcg aagccgtcac gaacgacctg aagccgcgcg acatcgtcac gcgcaagagc
     2161 atcgagaacg cggtcgcggt gatcatggcc accggcggct cgaccaacgc ggtgctgcac
     2221 ttcctcgcga tcgcgcacgc ggccgaggtc gagtggacga tcgacgactt cgagcgcgtg
     2281 aggcagggcg tgccggtgct gtgcgacctg aaaccctcgg gccagtacct cgcggtcgac
     2341 ctgcatcgcg ccggcggcat cccgcaggtg atgaagacgc tgctcgccgc cggcctgttg
     2401 cacggcgact gcatcacgat caccggcaag accgtggccg agaacctggc cgcgattccc
     2461 gacgcgccgc ggaccgacca ggacgtgatc cggccgatcg gcaggcccat gtacgagcac
     2521 ggccacctcg cgatcctcaa gggcaatctc tcgcccgagg ggtcggtcgc caagatcacc
     2581 gggctcaaga acccggtca
//
LOCUS       DASRDG010000131         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1403207, whole genome shotgun sequence.
ACCESSION   DASRDG010000131 DASRDG010000000
VERSION     DASRDG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403207"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1124
                     /locus_tag="VFA35_02165"
     CDS             123..1124
                     /locus_tag="VFA35_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZT55003.1"
                     /translation="MPASNHLVTVSTQPCSMPIARMRKLYRVDQVGAKLDKLPHKEHE
                     SLRSTYERMLEKGPERFQVKPAGLPVMDHLYDELPNFSDVLDDIRRQLALCEDSGDAL
                     EITPLLLLGPPGVGKTHFARELSVLLGTGMGFMSMSSMTAGWLLSGASSQWKGARPGK
                     VFETLVDGQYANPVMVVDEIDKAGGEHAYDPLGALYSLLEHDTAGGFTDEFAEVPIDA
                     SQVIWVATANDGRAIPDPILNRMNVYEVQAPDRDAARRIALRLYRGIRAEHGWGERFD
                     PEPGDAVLDAMSEMAPREMRRAWMTAFGNARLDRRASLTTRDLPETRGKRGAIGFVQ"
     gene            complement(1712..2440)
                     /locus_tag="VFA35_02170"
     CDS             complement(1712..2440)
                     /locus_tag="VFA35_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZT55004.1"
                     /translation="MRLVAKPYLQSVSIKPDAEPDFDRHPHNVPAVRELGQIDFHPDV
                     TFFVGENGSGKSTVLEAIALALGFGPEGGTRNVLLLKTVESVSSLHQSLRLARSFRKP
                     VDGYFLRAESFFNVATYMDGLPGNTDPLLHPRSHGEAFMSLLTDKFRGKGLYLLDEPE
                     AALSPNRQLAALSAIHQLVQDESQFIIATHSPILLAYPNAKILLFDGTGITEVAYEDT
                     EHFAVMRDFLNHYPRRLVQLLDGE"
     gene            2618..>2919
                     /locus_tag="VFA35_02175"
     CDS             2618..>2919
                     /locus_tag="VFA35_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HZT55005.1"
                     /translation="MPTTLDGQLVVAISSRALFDFEEENRHFEANDDRAYMQLQLERL
                     DRAAKPGVAFSLVNKLLAFNAGAPPQQRVEVVILSRNDPVSGMRVFRSAQHYGLPI"
BASE COUNT          495 a          984 c         1001 g          439 t
ORIGIN      
        1 aagtaggccg gcctggcatg cgatctgcaa caacagggcg gtgattgcgc cggtgttcaa
       61 gcggcggtgg aacgcaatcc gtgcttgaat gatctcagtg catgtcgaag gaggatcgct
      121 cgatgcccgc ttccaaccac ctggtcacgg tcagcaccca gccctgcagc atgccgatcg
      181 cgcgcatgcg caagctctac cgtgtcgacc aggtcggcgc caagctcgac aagctgccgc
      241 acaaggaaca cgagagcctg cgttccacct acgaacgcat gctcgagaag ggccccgagc
      301 gctttcaggt caagcccgcc gggctaccgg tgatggacca cctctacgac gagctgccca
      361 acttcagcga cgtgctcgac gacatccgcc gccagctcgc gctgtgcgaa gactcgggcg
      421 acgcgctcga gatcacgccg ctgctgctgc tcggcccgcc gggcgtgggc aagacgcact
      481 tcgcgcgcga actctcggtg ctgctcggca ccggcatggg cttcatgtcg atgagctcga
      541 tgacggccgg ctggctgctg tcgggcgcgt cgagccaatg gaaaggcgca cgccccggca
      601 aggtgttcga gacactggtc gacggccagt acgcgaaccc ggtgatggtc gtcgacgaga
      661 tcgacaaggc cgggggcgaa cacgcctacg acccgctcgg cgcgctctac agcctgctcg
      721 aacacgacac cgcgggcggc ttcaccgacg agtttgccga ggtgccgatc gacgcgagcc
      781 aggtgatctg ggtcgcgacc gcgaacgacg gccgggcgat tcccgacccg atcctgaacc
      841 ggatgaacgt ctacgaggtg caggcacccg accgcgacgc ggcgcgccgc atcgccttgc
      901 ggctgtaccg cggcatccgc gccgagcacg gctggggcga gcgcttcgac cccgagccgg
      961 gcgacgccgt gctcgacgcg atgagcgaga tggcgccgcg cgagatgcgc cgcgcgtgga
     1021 tgacggcctt cggcaacgcc cggctcgacc ggcgcgcgtc actcacgacc cgcgacctgc
     1081 cggagacgcg cggcaaacgc ggcgcgatcg ggttcgtgca gtagagcggg ctacgcgtcg
     1141 cgcagcgacg cgctctcgcc ggccacacgg tcggccatgc cgggggcagc gcgtcggctt
     1201 tgaccaggtc cacgcgaaac ggcaggtcgc tgaccccgaa ctgatcgcgc aggtcggcgc
     1261 gctgcgacca gctcagccgc gccggccgaa ccaccagcag gtcgaggtcg gaaaacggcc
     1321 gcgcccggcc ggtcgcgcgc gagccgaaca cgcgcacctg ggcgccggga acaacccgat
     1381 ccacgatgtc gcacgcggcg cgccgctgcg ccggcgtcag gtccaccgag cggcgagccg
     1441 gcgcagcctc atttggcaag gcccggcctc catcacctcg agaacgcgat ggccttgcgc
     1501 aacggcgtga ggtcgatggt cggcgaggtc atggcggatt gtcggcgagc accggtcgcg
     1561 gcgtcaaaac gcagatgagc ctgggcttgt ccacggcttg aaagtggtcg agtcgcgcgc
     1621 gtcgtcaagg ggtgactgca acgccagctc ttgccccagg tgctccgcga gaaccggcag
     1681 attctgcatc agcgtgtcgt aaccagcgcc ttcactcgcc gtcgagcaac tgcaccagcc
     1741 ggcgtgggta gtggttcaga aaatcgcgca tgaccgcgaa gtgctcggtg tcttcgtacg
     1801 caacctcggt gatgcccgtg ccgtcgaaca gcaggatctt tgcgttcggg taggcgagca
     1861 ggatcggcga gtgcgtcgcg atgatgaact gcgactcgtc ctgaaccagc tggtggatcg
     1921 cgctgagcgc ggcaagctgg cggttcggtg acaacgcggc ctcgggctcg tcgagcaggt
     1981 acaagccctt gccgcggaac ttgtcggtga gcagcgacat gaaagcttcg ccgtgcgagc
     2041 gcgggtgcag aagcgggtcg gtgttgcccg gcagtccgtc catgtaggtc gcgacgttga
     2101 aaaagctctc ggcgcgcaga aagtacccgt cgacgggctt gcggaagctg cgcgccagcc
     2161 gcagcgactg gtgcagcgac gacaccgatt cgactgtctt cagcagcagc acgttgcgcg
     2221 ttccgccctc gggcccgaag ccgagcgcga gggcgatggc ttcgagcacg gtcgacttgc
     2281 cgctgccgtt ctcgccgaca aagaaggtca cgtcggggtg gaagtcgatc tgcccgagct
     2341 cgcgcaccgc gggcacgttg tgcgggtggc ggtcgaagtc gggctcggca tcgggcttga
     2401 tcgagaccga ctggagatag ggcttcgcaa ccagcctcat ctggcggcgc tcccgccgct
     2461 cgggccttcg cccgcgagtc ggatgccggg tgtaacgatc agcatcggcg cagctccctt
     2521 cccttgacga agtatggcgt gtgccgtgtg tgcatgccgc agcgtaacgc aacctcggcc
     2581 gcgcgaggtc cgcgcggccg ctcgtatcat cggccgcatg cccaccaccc tggacggtca
     2641 actcgtcgtc gcgatctcgt cgcgcgcgct cttcgacttc gaggaggaaa accggcactt
     2701 cgaggccaac gacgaccgcg cctacatgca gctgcagctc gagcggctcg accgcgccgc
     2761 gaagccgggc gtcgcgttct cgctcgtgaa caagctgctg gcgttcaatg ccggcgcacc
     2821 gccacagcag cgcgtggagg tcgtgatcct gtcgcgcaac gacccggtct cgggcatgcg
     2881 cgtgttccgc tcggcgcagc actacgggct gccgatcca
//
LOCUS       DASRDG010000132         4991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_944933, whole genome shotgun sequence.
ACCESSION   DASRDG010000132 DASRDG010000000
VERSION     DASRDG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944933"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /gene="groEL"
                     /locus_tag="VFA35_02180"
     CDS             <1..876
                     /gene="groEL"
                     /locus_tag="VFA35_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZT55006.1"
                     /translation="LATLVVNTIRGILKVVAVKAPGFGDRRKAMLEDIAILTGGKVIA
                     EEVGLTLEKVTLADLGQAKRVEVGKENTTIIDGAGAAGDIEARVKQVRIQIEEATSDY
                     DREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVAGGGVA
                     LLRAKQSAGVIKGDNADQEAGIKLVLKAIESPLREIVFNAGGEASVVVAAVLAGKGNY
                     GFNAANDTYGDMIEMGILDPTKVTRTALQNAASVASLMLTTEAMISEAPKDEAAGAGM
                     PGGMGGMGGMGGMDM"
     gene            1007..3154
                     /locus_tag="VFA35_02185"
     CDS             1007..3154
                     /locus_tag="VFA35_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HZT55007.1"
                     /translation="MPISPALEQFVDDELALAPALIERVVAGTLQLLRDAKDIRPSPP
                     ERAHRLAVAGALQSHDAAYQRSFLGALQSGVRASVTEQYDGPAADAPAAGGGLELMDE
                     SRVEIDIEISRAMQSIDTTAEWEQRELQTFTSTLVGQTHVSAESNPFRPLVYAAALWE
                     AACTVSPVQTHRTTLLRVSAGVAAGLLKKAWAAACTRLEAHGVEPGIYRTVLMAPGSV
                     PGRGPAPADPARPDAMAVLLASMPGGTNRFPTGGTALAMGAPSPRRPSAEFEQALARL
                     DELLRHLPPAGATAREQADMLVTRLNLHRSALIASAAVPVERQIIELLSRLFDSVLAD
                     RQVPAPFASVLARLQTSVLRVALRDTEVFDSSQHAVWRLLDRIGEAGAGSPHASDPRA
                     AALLALCHALAEQLAKAPAPDAGLYRRALTQLDTFLDHQLQTQLRAAQPAVQTLQLAE
                     RREILQQQLAHRLAEQMVPVRTTPGMRRFVTGAWALVLAESMLRDGEQGEATRGYIKL
                     VDELLWSLRLPDHPRSRQRLLALLPGMLQRLRAGMALIHLPAAEQVQVLDELMAIHTD
                     ALRPGGHTDPAALTPAQIVQRMRDEVLPPSTGHGGFRDSVLDLGSMETVPAELMPESP
                     SDEPAKRVDALRQADRLRLFLQGRWARVQLLWRSDQGLFFLFAGESAARTHSVTHRAL
                     EKLAAAGLLQALEARSLVQRALDNVTRELARPS"
     gene            complement(3151..4065)
                     /locus_tag="VFA35_02190"
     CDS             complement(3151..4065)
                     /locus_tag="VFA35_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT55008.1"
                     /translation="MIQTFDAVLPHGITLACRATGAPGAPVLVFLHGFPEAAFVWDEI
                     LEHFGERYRCIAPNLRGFGPSSAPVEPEQYRVKHLMADLEALIAQQAGGGPIEALVAH
                     DWGGAVAWTLAALKPELMKRLVIINSPHPATFLRELQHSPQQQAASAYMNFLCRPDAE
                     RLLAENDYARMWPMFENMGAADPAREGGGWLTAPVRDQYRAIWNVGLTGGCNYYRASP
                     LRPPTAADAAVLQIEFAPEFVTVRRPTLVIWAEDDTALPTPLIDGLDAFVPDMRLVRV
                     PGATHWIIHERPDFIAAEIEKHLAPRAG"
     gene            complement(4062..4733)
                     /gene="yihA"
                     /locus_tag="VFA35_02195"
     CDS             complement(4062..4733)
                     /gene="yihA"
                     /locus_tag="VFA35_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZT55009.1"
                     /translation="MGGVVETAAEIEKRALGWCHTARFRTTASQLDQLPQIELPEIAF
                     VGRSNAGKSTAINTLTQQKRLAFASKTPGRTQHINLFDLGPKIVPDSIFADLPGYGYA
                     AVARGAKLRWQKVMADYLAVRRSLVGVVLMVDSRLGFTDLDQQLMSFVAPRVGNGSVK
                     LLVLLTKSDKLNRAEAAAALAGAQAVLGEIATDEADVQVALFSALKKSGVGDAATALH
                     NWVHP"
     gene            4774..>4991
                     /locus_tag="VFA35_02200"
     CDS             4774..>4991
                     /locus_tag="VFA35_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55010.1"
                     /translation="MKSPKAVIVKSPGFAQENAAPMKSVASVVACLLLAVSASVGAAE
                     AAKPAFKPDLAKGQATSTNVCAACHTADG"
BASE COUNT          773 a         1739 c         1747 g          732 t
ORIGIN      
        1 ctcgcgacgc tggtcgtgaa cacgatccgc ggcatcctga aggtcgtcgc cgtcaaggcg
       61 ccgggcttcg gtgaccgtcg caaggcgatg ctcgaagaca tcgcgatcct gaccggcggc
      121 aaggtcattg ccgaagaagt cggcctgacg ctcgagaagg tgacgctggc cgatctgggc
      181 caggccaagc gcgtcgaagt gggcaaggaa aacaccacga tcatcgacgg cgccggcgcg
      241 gcgggcgaca tcgaagcacg cgtcaagcag gtgcgtatcc agatcgagga agccaccagc
      301 gactacgacc gcgagaagct gcaagagcgc gtggccaagc tggccggcgg tgttgccgtg
      361 atcaaggtcg gcgctgcgac cgaagtcgag atgaaggaaa agaaggcccg cgtagaagat
      421 gcgctgcacg caacccgtgc cgccgtcgaa gagggcatcg tggccggtgg tggcgtcgcg
      481 ctgttgcgcg ccaagcaatc ggccggcgtg atcaagggcg acaacgccga ccaggaagcc
      541 ggcatcaagc tggtgctgaa ggcgatcgaa tcgccgctgc gcgagatcgt cttcaacgcc
      601 ggcggcgaag ccagcgtggt cgtcgctgcg gtgttggccg gcaagggcaa ctacggcttc
      661 aacgcggcca acgacaccta tggcgacatg atcgaaatgg gcatcctgga cccgaccaag
      721 gtgacgcgca cggccctgca aaacgcggcc tcggtcgcgt cgctgatgct gacgaccgaa
      781 gcgatgatct ccgaagcgcc gaaggacgag gcggccggcg ccggcatgcc cggtggcatg
      841 ggcggcatgg gtggcatggg cggcatggac atgtaagccc ggtgccgccg cgacggcggc
      901 atccgcttgc atcaaaaggg tcacttcggt gacccttttt cgttggcgaa tcgattcctg
      961 gggcggcatt cgataatcgg cgcactccga ttctgaaggg cccccgatgc cgatttcccc
     1021 ggcgctcgag caattcgtcg atgatgaact ggcgctcgcg ccggcgctga tcgagcgcgt
     1081 ggtcgccggc acgctgcaac tgctgcgcga cgcgaaagac atccgcccga gcccgcccga
     1141 acgcgcgcat cggctcgcgg tcgccggggc gctgcagtcg cacgacgcgg cgtatcagcg
     1201 cagcttcctc ggcgcgctgc aatcgggggt gcgcgcgtcg gtcaccgagc agtacgatgg
     1261 ccccgcggcc gacgcacccg ccgccggcgg cgggctggag ctcatggacg agtcgcgggt
     1321 cgagatcgac atcgagatct cccgcgcgat gcagtcgatc gacaccaccg ccgagtggga
     1381 gcagcgcgag ctgcagacct tcacctcgac cctcgtcggc cagacccatg tcagcgccga
     1441 gtcgaacccg tttcgcccgc tcgtctacgc ggccgcgctg tgggaggccg cctgcacggt
     1501 cagcccggtt cagacgcatc gaacgacgct gttgcgcgtg tcggccggcg tcgcggcggg
     1561 cctgctcaag aaagcctggg cggccgcctg cacgcggctc gaagcgcacg gcgtcgagcc
     1621 cggcatctac cgcactgtgc tgatggcgcc gggctcggtg ccgggtcgtg gtccggcgcc
     1681 ggccgatccg gctcgccctg acgcgatggc tgtcctgctc gccagcatgc cggggggcac
     1741 gaaccgcttc ccgaccggcg gcacggcctt ggcgatgggc gcaccgtcgc cccgccggcc
     1801 gagcgccgag ttcgagcagg ccttggcccg gctcgacgaa ctcttgcgcc acctgccgcc
     1861 agccggcgcc accgcgcgcg agcaggccga catgctggtc acgcggctga acctgcaccg
     1921 ctcggcgctg atcgcgagcg ccgcggtgcc ggtcgagcgg cagatcatcg agttgctgtc
     1981 gcggctgttc gacagcgtcc ttgccgaccg gcaggtgccg gcgccctttg ccagcgtgct
     2041 ggcgcggctg cagacctcgg tgttacgcgt tgcgctgcgt gacacggaag tgttcgactc
     2101 gagccagcac gcagtctggc gcctgctcga ccgcatcggc gaagccggcg ccggctcccc
     2161 gcacgcgagc gatccgcgcg cggcggccct gctcgcgctg tgccacgcgc tggccgagca
     2221 gctcgcgaag gcgccggcgc ccgatgccgg gctctaccgc cgcgccctga cccagctcga
     2281 taccttcctc gaccaccagt tgcagaccca gctgcgcgcg gcgcagccgg cagtgcagac
     2341 gctgcaactg gccgagcggc gcgaaatcct gcagcagcag ctcgcgcacc ggctcgccga
     2401 gcagatggtg cccgtccgca ccacgccggg catgcgccgc ttcgtgaccg gcgcgtgggc
     2461 gctggtgctg gccgagtcga tgctgcgcga cggcgagcag ggcgaggcca cgcgcggcta
     2521 catcaagctg gtcgacgagt tgctgtggag tctgcgcttg cccgaccacc cgcggagccg
     2581 ccagcgcctg ctcgcgctgc tgcccggcat gctgcagcgg ctgcgcgccg gcatggcgct
     2641 gatccaccta ccggccgccg aacaggtgca ggtgctcgac gaattgatgg cgatccacac
     2701 cgatgcgctg cgccccggcg gccacaccga tccggccgcg ctgacgccgg cgcagatcgt
     2761 gcaacgcatg cgcgacgagg tgctgccgcc ctcgaccggc cacggcggct tccgcgattc
     2821 ggtgctcgac ctcgggtcga tggagaccgt accggccgaa ctgatgcccg agtcgccctc
     2881 cgacgagccc gcgaaacgcg tcgacgcgct gcgccaggca gaccgcttgc gcctgttcct
     2941 gcaaggccgc tgggcgcgcg tgcagctgct gtggcgcagc gaccaggggt tgttcttcct
     3001 gttcgccggc gagagcgcgg cgcgcacgca ctcggtcacg caccgcgcgc tcgagaagct
     3061 cgccgcggcc ggcctgctgc aagcgctgga ggcgcgttcg ctggtccagc gcgcgctcga
     3121 caacgtgacg cgcgagctgg cgcggccttc ctagcctgcc cgaggggcga ggtgcttctc
     3181 gatctcggcc gcgatgaagt cgggccgctc gtggatgatc cagtgcgtcg cgccgggcac
     3241 gcgcaccagg cgcatgtcgg gcacgaaggc gtcgagcccg tcgatcagcg gcgtcggcaa
     3301 cgcggtgtcg tcctcggccc agatcaccag ggtcggccgc ctcacggtga cgaactcggg
     3361 cgcgaactcg atctgcagca ccgctgcgtc ggccgcggtc ggcggccgca gcggcgaggc
     3421 gcggtaatag ttgcagccgc cggtcaggcc cacgttccag atcgcacggt actggtcacg
     3481 caccggcgcg gtcagccagc cgccgccttc gcgcgccggg tcggcggcgc ccatgttctc
     3541 gaacatcggc cacatgcggg cgtagtcgtt ctcggcgagc agccgctcgg catcgggccg
     3601 gcacaggaag ttcatgtagg cgctggccgc ctgctgctgc gggctgtgct gcagctcgcg
     3661 cagaaaggtc gcagggtgcg gcgaattgat gatcacgagg cgcttcatca actcgggctt
     3721 cagggcggcg agcgtccagg cgaccgcacc gccccagtcg tgcgcgacca gcgcctcgat
     3781 cgggccaccg ccggcctgtt gcgcgatcaa cgcctccagg tcggccatca ggtgcttgac
     3841 gcggtactgc tcgggttcga ccggcgcgga cgacggcccg aagccgcgca ggttcggcgc
     3901 gatgcagcgg tagcgctcgc cgaagtgttc gaggatctcg tcccagacga aggccgcttc
     3961 cgggaaaccg tgcaggaaca cgagcaccgg cgcgcccggc gcgcctgtgg cccggcaagc
     4021 cagcgtgatg ccatgcggca gcaccgcgtc gaaggtctga atcatgggtg aacccagttg
     4081 tggagggcgg ttgcggcgtc gccgacgccg gatttcttca gtgccgagaa cagcgcgacc
     4141 tgcacgtcgg cctcgtcggt cgcgatctcg cccagcaccg cctgcgcgcc ggccagcgcc
     4201 gcggcggcct cggcgcggtt gagcttgtcg gacttggtca gcagcaccag cagcttgacc
     4261 gaaccgttgc cgacgcgcgg cgcaacgaag ctcatcagct gctggtcgag gtcggtgaaa
     4321 cccaggcgcg agtcgaccat cagcaccacc ccgaccaagc tgcggcgcac cgccaggtag
     4381 tcggccatca ccttttgcca gcgcagcttc gcgccgcgcg ccaccgccgc gtagccgtag
     4441 ccgggcaggt cggcgaagat cgagtccggc acgatcttcg ggccgaggtc gaacaggttg
     4501 atgtgctggg tgcggccggg cgtcttcgac gcgaacgcca ggcgcttctg ctgtgtcaga
     4561 gtgttgatcg cggtcgactt gcccgcgttc gagcggccga cgaacgcgat ctccggcagc
     4621 tcgatctgcg gcaactggtc gagctggctg gcggtggtgc gaaagcgcgc ggtgtggcac
     4681 caacccagcg cgcgcttttc gatctcggca gcggtctcga cgacgcctcc catgcccgcg
     4741 accgcggcct gacttgaatt gttttgctcg gccatgaagt cgcccaaagc cgtcattgta
     4801 aaatcaccgg gttttgcaca agaaaacgct gcacccatga agtccgttgc ctccgtcgtt
     4861 gcctgcctgc tgctcgccgt gtctgcgtcg gtcggcgccg cggaagcggc caaacccgcg
     4921 ttcaagcccg atctggccaa gggtcaagcc acgtcgacca acgtctgcgc cgcatgccac
     4981 accgccgacg g
//
LOCUS       DASRDG010000133         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_592330, whole genome shotgun sequence.
ACCESSION   DASRDG010000133 DASRDG010000000
VERSION     DASRDG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592330"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFA35_02205"
     CDS             complement(<1..452)
                     /locus_tag="VFA35_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55011.1"
                     /translation="MGIDLHNLGLLAHAQDRGVAFERSVGIGRQVLFVDPPELEQHRG
                     LRGLQALREPAPPPGSPRYFEPLMQQWFGARSVDSVDASAYEQASVLHDMNLPWAEGQ
                     PGLGSYDAVLDFGCLEHVFDFPVAWRNCVALCKIGGHLFHSLPANNLSG"
     gene            complement(460..1689)
                     /locus_tag="VFA35_02210"
     CDS             complement(460..1689)
                     /locus_tag="VFA35_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZT55012.1"
                     /translation="MPQVYAAAAVRLALPLVVLPLVATRVGAAEFGRLGFILVWAGLL
                     ATLVEGGFLAAATRLAVAADAAARWRLARQVFSARLALSLPAVLLAFVAVGWAAPGAA
                     PPLGDAVAIALLACALGWPATWYLQATQQLNRWSRVEVGVYAALIVGCWAFATSVLAF
                     VVLQLAASALLAVLGWRWLAADLAEVRRGERLFDRAALGPGLRLGWKMLPVSIAGAAY
                     SLALPAAASAQLSKPDLGVYFMADRIVRAVLAVADPVYSLVYPRMVALFASSSRSALW
                     YAARWAALGSVAGAVLFLLVQSFWPLLEPFLAARAGSIDVQQVHATALVLAWLWPLLL
                     GWKFFGYWMLGSGRHDGAYRLCIVTGGIVGVAMAATVGGAHGASGLAWTSIGVELLVI
                     VVAVTAMAASRLRRRIG"
     gene            complement(1734..>2221)
                     /locus_tag="VFA35_02215"
     CDS             complement(1734..>2221)
                     /locus_tag="VFA35_02215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HZT55013.1"
                     /translation="RGDTSRDLWLYDTFDGMSEPGAEDRSTSGESAAAQLARTPRGEG
                     VWCEAGLADVRANLESTGYPLERIHFVEGKVEDTIPASLPKRAALLRLDTDWYESTRH
                     ELCHLYPLLSRHGILVIDDYGHWQGARQAVDEYFAAQAQPVYLHRVDYTARLVVKQDG
                     A"
BASE COUNT          359 a          803 c          818 g          241 t
ORIGIN      
        1 ccgctcaggt tgttggccgg cagcgaatgg aacaggtggc cgccgatctt gcagagggcg
       61 acgcagttgc gccaggcgac cgggaagtcg aacacgtgtt cgaggcagcc gaagtcgagc
      121 accgcgtcgt agctgccgag cccgggctgg ccttcggccc acggcaggtt catgtcgtgc
      181 agcacgctcg cctgctcgta tgccgaggcg tcgaccgaat cgacgctgcg agcaccgaac
      241 cactgctgca tcagcggctc gaagtagcgc ggcgaaccgg gcggcggcgc cggctcgcgc
      301 aaggcttgca ggccgcggag tccccggtgc tgctcgagct cgggcgggtc gacgaacagc
      361 acctggcggc cgatgccgac gctgcgctcg aaggcgacgc cgcggtcctg cgcgtgggcg
      421 agcaggccga ggttgtgcag gtcgatgccc atggcgtcct cagccgatgc gccggcgcag
      481 gcggctcgcc gccatcgccg tgaccgcgac gacgatcacc agcagctcga cgccgatcga
      541 tgtccaggcc aggcccgaag cgccgtgcgc accgccgacc gtcgccgcca tcgccacgcc
      601 gacgatgccg ccggtgacga tgcagagacg ataggcgccg tcgtgccggc cgctgccgag
      661 catccagtag ccgaagaact tccagccgag caggagcggc cagagccacg ccaggaccag
      721 ggctgtggca tgcacctgct gcacgtcgat gctgccggcg cgggccgcga ggaagggctc
      781 gagcagcggc cagaacgact gcaccagcag gaacagcacc gccccggcga cgctgccgag
      841 cgccgcccag cgcgccgcgt accagagggc ggatcgcgag ctgcttgcga acagggcgac
      901 catgcgcgga tagacgagcg aatacaccgg gtcggcgacg gcaagcacgg cgcgcacgat
      961 ccggtcggcc atgaagtaga cgccgaggtc gggcttgctc agctgcgccg acgcggccgc
     1021 cggcaaggcc agcgagtacg cggccccggc gatcgacacc ggcagcatct tccagccgag
     1081 gcgcaggccg gggccgaggg ccgcgcggtc gaacagcctt tcgccccggc gaacttcggc
     1141 gagatcggcg gccagccagc gccagccgag cacggcgagc agcgccgagg cggccagctg
     1201 gaggacgacg aacgcgagca cgctggtcgc gaacgcccag cagccgacga tcagcgcggc
     1261 gtagaccccc acctcgaccc gcgaccagcg gttgagctgc tgcgttgcct gcaggtacca
     1321 ggtggccggc cagccgaggg cgcaggcgag caaggcgatc gcgaccgcat cgccgagcgg
     1381 cggcgccgcc ccgggcgcgg cccagccgac ggcgacgaag gcgagcagca ccgccggcag
     1441 cgagagcgcg aggcgggcgc tgaacacctg ccgggcgagg cgccagcgcg ccgccgcgtc
     1501 ggcggcgacc gcgagccgcg tcgccgccgc gaggaaaccg ccttcgacca gcgtcgccag
     1561 gagcccggcc cacaccagga tgaagccgag ccggccgaac tcggccgcgc cgacgcgcgt
     1621 cgcgaccagc ggcagcacca ccagcggcag cgccagccgc accgctgccg ccgcgtagac
     1681 ctgcggcaag gcagtcagca gccgcggggc ggaagggatt cccccgcggg cgttcatgcg
     1741 ccgtcttgct tcaccacgag ccgcgccgtg tagtcgacgc ggtgcaggta gaccggctga
     1801 gcctgcgcgg cgaagtactc gtcgaccgcc tggcgcgcgc cctgccaatg gccgtagtcg
     1861 tcgatcacca ggatgccgtg gcgcgacagc agcggataga gatggcagag ctcgtgccgc
     1921 gtcgactcgt accagtcggt gtcgagccgg agcagcgccg cgcgcttcgg caggctggcg
     1981 ggaatcgtgt cctcgacctt gccctcgacg aagtggatgc gctcgagcgg gtagccggtc
     2041 gattcgaggt tggcccgcac gtccgcgagg ccggcctcgc accacacgcc ctcgccgcgc
     2101 ggcgtgcgcg cgagctgcgc cgccgccgat tcgccgctcg tgctcctgtc ctcggcgccg
     2161 ggctcgctca tgccgtcgaa ggtgtcgtag agccagagat cgcgcgaggt gtcgccgcgc
     2221 g
//
LOCUS       DASRDG010000134         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_477967, whole genome shotgun sequence.
ACCESSION   DASRDG010000134 DASRDG010000000
VERSION     DASRDG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477967"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="proS"
                     /locus_tag="VFA35_02220"
     CDS             complement(<1..798)
                     /gene="proS"
                     /locus_tag="VFA35_02220"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZT55014.1"
                     /translation="MKASQFFISTLKEAPADAEVASHKLMMRAGFIKRLGAGIYNYMP
                     MGLRVIRKVENIIREEMNRAGAIELLMPVVQPAELWQETGRFQKYGAELLRLKDRHDR
                     DFVIQPTSEEVITDIARQELRSYRTLPRNFYHIQTKFRDERRPRFGVMRGREFTMKDA
                     YSFDRDIASASKSYDIMFNAYTRIFERMGLAFRAVAADNGSIGGVRSHEFQVIADTGE
                     DALVYCPTSDYAANVELAEALALGTRSAPAAPMTKTATPGKATCADVA"
     gene            932..1534
                     /locus_tag="VFA35_02225"
     CDS             932..1534
                     /locus_tag="VFA35_02225"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HZT55015.1"
                     /translation="MLDREGFRPNVGIVLLNQRNQVFWGKRIRTHSWQFPQGGIKYGE
                     TPEQAMFRELHEEVGLVPEHVRIVARTRDWLRYEVPDHFIRRDARGHYRGQKQIWFLL
                     QLVGRDSDMNLRATDHPEFDAWRWNEYWVPLELVIEFKRDVYQMALTELARFLPRGAH
                     HNRYLRSGMRPHHRDEGPMRHDEPAPPAVVPLPAVGKLPE"
     gene            complement(1629..>2083)
                     /locus_tag="VFA35_02230"
     CDS             complement(1629..>2083)
                     /locus_tag="VFA35_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HZT55016.1"
                     /translation="LAAKRAASSGASTVIAYGREVDVLIRLAGGESIGTALIAPTQKL
                     AARKQWMVDHLQLRGTVVIDDGAARKVRDEGKSLLPIGVTDVQGEFHRGDVIAVRGSN
                     GVELARGLANYGSGEARLIARRPSSEFEKLLGYTAEPELIHRDNMVLA"
BASE COUNT          326 a          703 c          693 g          361 t
ORIGIN      
        1 cgccacgtcc gcgcacgtgg ccttgcccgg cgtggccgtc ttcgtcatcg gcgcggcggg
       61 tgccgagcgc gtgccgagcg ccagcgcttc ggccagctcg acgttggccg cgtagtcgct
      121 ggtcgggcag tagacgagcg cgtcctcgcc ggtgtcggcg atcacctgga actcgtgcga
      181 gcgcacgccg ccgatcgagc cgttgtcggc cgcgaccgcg cggaaggcca gccccatgcg
      241 ctcgaagatg cgcgtgtagg cgttgaacat gatgtcgtag ctcttgctcg ccgaggcgat
      301 gtcgcggtcg aaggaatagg cgtccttcat cgtgaactcg cgcccgcgca tcacgccgaa
      361 gcgcgggcgg cgctcgtcgc ggaacttggt ctggatgtga tagaaatttc gcggcagggt
      421 gcggtagctg cgcaactcct ggcgcgcgat gtcggtgatg acttcttcgc tggtcggctg
      481 gatcacgaag tcgcggtcat gccggtcctt caggcgcagc aactcggcgc cgtacttctg
      541 aaagcggccc gtctcctgcc acagctcggc cggctgcacc accggcatca gcaactcgat
      601 cgcgccggcg cggttcatct cctcgcggat gatgttctcg accttgcgaa tcacgcgcaa
      661 gcccatcggc atgtagttgt agatgccggc gccgagccgc ttgatgaagc cggcacgcat
      721 catcagcttg tggctcgcga cttcggcatc ggccggcgct tctttcagcg tggagatgaa
      781 gaactgggat gctttcatgg gcgggcgcga aggggatgtg cggctgggcg cggcaccggt
      841 cgcgtggccc cgaagggctg cgggaaatcg aaggtttgaa tcccgtcacg ccggtgtaat
      901 aatgaattca acgaatgaat tggggttgat tatgctcgac cgtgaagggt tcagacccaa
      961 cgtcggcatc gtcctgctca accaacgaaa ccaggtgttt tggggcaagc gcatccgcac
     1021 ccactcctgg cagttcccgc aagggggcat caaatacggc gagacgcccg agcaggcgat
     1081 gttccgcgag ctccacgaag aagtgggcct cgtgcccgag catgtgcgca tcgtcgcgcg
     1141 cacgcgcgac tggctgcgct acgaggtgcc ggatcacttc atccggcgcg acgcgcgcgg
     1201 ccactaccgc gggcagaagc agatctggtt cctgctgcag ttggtcggtc gcgacagcga
     1261 catgaacctg cgcgccaccg accaccccga gttcgacgcc tggcgctgga acgagtactg
     1321 ggtgccgctg gaactcgtca tcgagttcaa gcgcgacgta taccagatgg cgctgaccga
     1381 actcgcgcgc ttcttgccac gcggcgcgca tcacaaccgc tacctgcgtt cggggatgcg
     1441 cccgcaccat cgtgacgagg gcccgatgcg ccacgacgag cctgcgccgc cggccgtcgt
     1501 gccgctgccc gcggtcggta agttgcccga gtaggcgccc ggctgacgcg gctacgccag
     1561 caccaggttg tggttccggc ccgcgccgcc ttcgcggcac taccctgcgc cgcaatatgg
     1621 ttctggcgtc aggccagaac catgttgtcg cggtggatga gctccggctc ggcggtgtag
     1681 ccgagcagtt tctcgaactc gctcgacggc cggcgcgcga tcaggcgcgc ctcgccgctg
     1741 ccgtagttcg ccaagccccg tgcgagctcg acaccgttcg acccgcgcac cgcgatcacg
     1801 tcgccgcgat gaaactcgcc ctgcacgtcg gtcacgccga tcggcagcag gctcttgcct
     1861 tcgtcgcgca ccttgcgcgc ggcgccatcg tcgatcacga ccgtgccgcg cagctgcagg
     1921 tggtcgacca tccactgctt gcgcgcggcg agcttctgcg tcggcgcgat caacgcggtg
     1981 ccaatcgact cgccgcccgc caggcggatc agcacgtcga cctcgcgccc gtacgcgatc
     2041 accgtgctcg caccgctgct ggccgcgcgc ttggcggcga gga
//
LOCUS       DASRDG010000135         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_254293, whole genome shotgun sequence.
ACCESSION   DASRDG010000135 DASRDG010000000
VERSION     DASRDG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254293"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /gene="metG"
                     /locus_tag="VFA35_02235"
     CDS             <1..1151
                     /gene="metG"
                     /locus_tag="VFA35_02235"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HZT55017.1"
                     /translation="IIYFHTLFWPAMLKFAGPPYKVPSHVWVHGFITLSGDKMSKSRG
                     TGLSPLRYLDVGMNPEWLRYYIAAKLNARVEDLDFNPDDFLARVNADLIGKYINIASR
                     AAGFLSKRFGGRLSNDLGVEGRTLLDGLRAQRATIEALYEEREFGKALREVMLLADRV
                     NEFVDQNKPWELAKLAGKDAVLHDVCSVCIEAFRLLTIYLKPVLPVLAAQVEAFLKVE
                     PLQFADVARAIGGHAIGEYSHLMQRVDAKQLETLIEPTVVEAPPPGGEPIAPEITVSD
                     FAKLDLRIAKIENCEAVEGSTKLLRLTLDVGEDKTRNVFSGIASAYQPNDLIGKFTVM
                     VANLAPRKMKFGVSEGMVLAAAHADEKAEPGLYILEPWPGAQPGMRVR"
     gene            complement(1164..2177)
                     /locus_tag="VFA35_02240"
     CDS             complement(1164..2177)
                     /locus_tag="VFA35_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55018.1"
                     /translation="MPAPPPAPLPPPSDGTPAAGYAAGGCAVPAAAQAAATTAPDHVV
                     GTGTPASCTSDAVVAAVAAGGVTTFNCGPDPVTITLTKTAKVFNDKPNVVLDGGGKVT
                     LSGGGALRILYQNTCDPAQVWTSARCDLQDTPQTTVQNLTLVDGNSSAQSYGQGEVYG
                     GGAIYVRGGRMKVVASRFFRNRCEASGPDLGGAALRAFGTSAPVYVVGSTFGGAAGYG
                     GQCSNGGALSGLGASFTVLNSLFSHNQATGSGGGGGGAIYMDGNAMNLSLCGNALHDN
                     SASEGGGALFYVSNDRTGTMSITNSLLQANISGKFETSGLPGIFALAAPGQPVVTNST
                     ISK"
BASE COUNT          353 a          785 c          795 g          365 t
ORIGIN      
        1 acatcatcta tttccacacg ctgttctggc cggcgatgct gaagttcgca ggcccgccgt
       61 acaaggttcc cagtcacgtg tgggtgcacg gcttcatcac cctttcgggc gacaagatgt
      121 cgaagtcgcg cggcacgggc ctctcgccgc tgcgctatct cgatgtcggc atgaatccgg
      181 aatggctgcg ctactacatc gcggccaagc tgaacgcgcg cgtcgaagac ctcgatttca
      241 acccggacga ctttctcgcg cgcgtcaatg ccgacctgat cggcaagtac atcaacatcg
      301 cgagccgtgc cgcgggcttt ctgagcaagc gtttcggcgg gcggctgtcg aacgacctcg
      361 gcgtcgaggg ccgcacgctg ctcgatgggc tgcgcgcgca gcgcgcgacg atcgaagcgc
      421 tctacgaaga acgcgagttc ggcaaggcgc tgcgcgaggt gatgctgctg gccgaccgcg
      481 tcaacgagtt cgtcgaccag aacaagccct gggaactcgc gaagctggcc ggcaaggacg
      541 ccgtgctgca cgacgtgtgc tcggtctgca tcgaggcgtt ccgcctgctg acgatctacc
      601 tgaagccggt gctgccggtg ctggccgcgc aggtcgaggc gttcctgaag gtcgagccct
      661 tgcagttcgc cgacgtcgcc cgcgcgatcg gcggccacgc catcggtgag tacagccacc
      721 tgatgcaacg cgtcgacgcg aagcagctcg aaacgctgat cgaacccacc gtcgtcgaag
      781 ctccgccacc cggcggcgag ccgatcgcgc ccgagatcac cgtctccgat ttcgcgaagc
      841 tcgacctgcg catcgcgaag atcgagaact gcgaagcggt cgagggttcg accaagctgc
      901 tgcgactcac gctcgatgtc ggcgaagaca agacccgcaa cgtgttcagc ggcatcgcct
      961 cggcgtatca gccgaacgac ctgatcggca agttcaccgt gatggtcgcg aacctcgcgc
     1021 cgcgcaagat gaagttcggt gtcagcgaag gcatggtgct cgcggccgcg cacgccgacg
     1081 agaaggccga gcccggcctg tacatcctcg agccctggcc gggcgcgcaa cccggcatgc
     1141 gcgtgcgctg aagtcgcggt tcgttacttc gagatcgtcg aattcgtgac gaccggctgc
     1201 cccggcgccg cgagcgcgaa gatgccgggc agcccgctgg tttcgaactt gccgctgatg
     1261 ttggcctgca gcagcgagtt ggtgatcgac atcgtgccgg tccggtcatt gctgacgtag
     1321 aacagcgcgc cgccgccttc gctcgcgctg ttgtcgtgca gcgcgttgcc gcacaggctc
     1381 aggttcatcg cgttgccgtc catgtagatc gcgccgccgc ctccgccgcc gctgccggtc
     1441 gcctggttgt ggctgaacag gctgttcagc accgtgaacg aggcgcccag gccgctcagt
     1501 gcgccgccgt tcgagcattg cccgccgtag cccgcggcac cgccgaaggt gctgcccacc
     1561 acatagaccg gcgccgaggt gccgaacgcg cgcagcgccg cgccgcccag atcgggcccg
     1621 ctcgcttcgc aacggttgcg gaagaagcgg gaggcgacga ccttcatgcg cccgccgcgc
     1681 acgtagatcg cgccgccgcc gtagacctcg ccctggccgt agctctgcgc gctcgagttg
     1741 ccgtccacga gggtcaggtt ctgcaccgtg gtctgcggcg tgtcctggag gtcgcagcgc
     1801 gcgctggtcc agacctgcgc cgggtcgcag gtgttctggt acaggatgcg cagcgcgccg
     1861 ccgccgctga gcgtgacctt gccgccaccg tcgagcacga cgttcggctt gtcgttgaag
     1921 accttcgcgg tcttcgtcag cgtgatcgtg accgggtcgg ggccgcagtt gaaggtcgtg
     1981 acaccgccgg cagcaacggc cgcgacgacg gcgtcggagg tacagctcgc cggcgtgccg
     2041 gtgccgacaa catggtcggg cgcggtcgtc gcggccgctt gcgccgccgc gggtacggcg
     2101 cagccgccgg ccgcgtagcc ggccgccggc gtgccgtccg acggcggcgg cagcggcgcg
     2161 ggaggcggtg ccggcaccgg gagcggaacc ggtgcaggtg caggtgcagg tgcaggtgca
     2221 ggtgcaggtg caggtcccgg cgatggcgcc ggcgcgggcg ctccgggcgc agcggccggc
     2281 gcatcgccac caccgcct
//
LOCUS       DASRDG010000136         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1628866, whole genome shotgun sequence.
ACCESSION   DASRDG010000136 DASRDG010000000
VERSION     DASRDG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628866"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1842)
                     /locus_tag="VFA35_02245"
     CDS             complement(13..1842)
                     /locus_tag="VFA35_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT55019.1"
                     /translation="MAEPAHSDNELERGILSSRLGRRLLTQLLGCALLPLSVFAWLSL
                     TQVSLQFRSEMERGLHASAKTAGMELADHVLAKAAAMDVVAEMALQGTGPELDARLRL
                     LRERLQLYFSALWIDDGGRVRALAGDPSPLSPLRKDQLAHLAKGKVLARAVAPGRDGH
                     PSVQLLRRLEPQNASSPLLAADLRADQLGDVESLRGHGAEVLVLTGDHRLDVLLSSIE
                     PAPDLGAMNLELALRPSSGTLPLDVRGAEQIASYWRVFLLPQLGMDLVVVQTRARSQA
                     EAPLRAFQVPFALTALLTLSVVAAVALFQVRHMLVPIRRLLATTRAVASGDLDARASL
                     EARDELGELGRSFDDMTCELVDNIRRRELTELDLIAARDGALEAVRVKAEFLTNVTHE
                     LRTPLTAIVSGAEILRQFGDDDPAVRAEFVTMVCDQAERLRSLIDGILELERTAPPAL
                     APVDIAASLRAAITMLPHEHRARVATDVGEPMVVQGDGERLTQLWAHLLDNAVKFSPP
                     GSPVHVRARRLDGACEVEVEDLGIGIAAADHQRIFEAFCQVGRNILTDKAAGIGLGLT
                     LARNIAESHRGSLTVVSAPGRGATFRVTLPLAAAAPAPALAPA"
     gene            complement(1853..>2135)
                     /locus_tag="VFA35_02250"
     CDS             complement(1853..>2135)
                     /locus_tag="VFA35_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55020.1"
                     /translation="HDYLKQEVEILNAASAGLARRVDALQQANAATVADLLRCSEALA
                     GGSDGACAALTSAQQNAREVGQLLAGFERIGGRDPSTAALEVAVAPCHC"
BASE COUNT          286 a          775 c          803 g          271 t
ORIGIN      
        1 gcactgcgcg cgctaggccg gcgcgagcgc tggcgcgggt gcggccgccg ccagcggcag
       61 ggtgacgcgg aacgtggcgc cgcggcccgg tgcgctgacg acggtgagcg agccgcggtg
      121 cgactcggcg atgttgcgcg ccagcgtgag gccgagcccg atgccggcgg ccttgtcggt
      181 caggatgttc cggccgacct ggcagaacgc ttcgaagatg cgctggtggt cagccgcggc
      241 gatgccgatg ccgaggtcct cgacctccac ctcgcacgcg ccgtcgagac gtcgcgcgcg
      301 aacgtgcacc ggggagccgg gcgggctgaa cttcacggcg ttgtcgagca ggtgcgccca
      361 cagctgcgtc aaccgctcgc cgtcgccttg cacgaccatc ggctccccga cgtcggtcgc
      421 gacgcgcgcg cggtgttcgt gcggcagcat ggtgatcgcg gcgcgcagcg acgccgcgat
      481 gtcgaccggc gccaaggccg gcggcgccgt ccgttcgagc tcgaggatgc cgtcgatgag
      541 gctgcgcagc cgctccgcct ggtcgcacac catggtgacg aactcggcgc gcacggcggg
      601 gtcgtcgtcg ccgaactggc gcaggatctc ggcgccgctg acgatcgccg tgagcggcgt
      661 gcgcagttcg tgcgtcacgt tggtcaggaa ctcggccttg acgcgcaccg cttcgagcgc
      721 gccgtcgcgc gcggcgatca ggtcgagctc ggtgagctcg cgccggcgga tgttgtcgac
      781 gagctcgcac gtcatgtcgt cgaacgagcg cccgagctcg ccgagctcgt cgcgcgcctc
      841 cagtgaggcg cgcgcgtcga ggtcgccgct cgccaccgcc cgcgtcgtcg cgagcagacg
      901 gcggatgggc accagcatgt ggcgcacctg gaacagcgca acggcggcga cgacggacag
      961 cgtgagcagc gccgtcagcg cgaacggcac ctggaaggcg cgcagcggcg cctcggcctg
     1021 gctgcgcgcg cgcgtctgca cgacgacgag gtccatcccg agctgcggca gcaggaacac
     1081 gcgccagtac gaggcgatct gctccgcgcc ccggacgtcc aggggcaacg tgccggagga
     1141 gggccgcagc gccagctcga ggttcatcgc gccgagatcg ggcgcgggct cgatcgagct
     1201 cagcagcacg tcgaggcggt ggtcgcccgt cagcacgagc acctcggcgc cgtggccgcg
     1261 cagcgactcg acgtcgccga gctggtccgc gcgcaggtcg gccgcgagca gcggcgagct
     1321 cgcgttctgg ggctcgaggc gtcgcagcag ctggaccgac gggtgcccgt cgcggccggg
     1381 cgcgaccgcg cgcgcgagca ccttgccctt cgcgaggtgc gccagctggt ccttgcgcaa
     1441 cggcgacagc ggcgacggat cgcccgccag cgcgcgcacg cggccgccgt cgtcgatcca
     1501 gagcgcggag aagtagagct gcaggcgttc gcgcaggagc cgcaggcggg cgtcgagctc
     1561 gggcccggtg ccctgcagcg ccatctcggc gacgacgtcc atggccgcgg ccttcgcgag
     1621 cacgtggtcc gccagctcca tgcctgccgt cttcgcgctc gcgtgcaggc cgcgctccat
     1681 ctcgctgcgg aactgcagcg agacctgcgt cagcgacagc cacgcgaaga ccgacagcgg
     1741 caacagcgcg cagcccagga gctgcgtgag cagccgccgg ccgagccggc tgctcaggat
     1801 gccgcgctcg agctcgttgt cggaatgggc cggttccgcc acggcacgat cctcagcagt
     1861 ggcacggggc gacggcgacc tccagagcgg cggtcgacgg gtcgcgcccg ccgatgcgtt
     1921 cgaaaccggc cagcagctgg ccgacctccc gcgcgttctg ctgggccgag gtcagcgccg
     1981 cgcacgcgcc gtcgctgccg ccggccaggg cctcgctgca gcgcagcagg tccgcgaccg
     2041 tcgccgcgtt cgcctgctgc agcgcgtcga cgcgccgtgc cagccccgca gacgccgcgt
     2101 tgaggatctc cacctcctgc ttgaggtagt cgtgc
//
LOCUS       DASRDG010000137         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1052845, whole genome shotgun sequence.
ACCESSION   DASRDG010000137 DASRDG010000000
VERSION     DASRDG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052845"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..753
                     /locus_tag="VFA35_02255"
     CDS             70..753
                     /locus_tag="VFA35_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZT55021.1"
                     /translation="MKKYLIAGLLVWLPMAVTIWVLQAALGLLDGVFGWLLNAGRAIL
                     PAAAEPLIDLLLKIPGLGVLVMLLGLLLTGVFATNMAGQWFLRQGHRLLNKIPIVKSI
                     YSSVKQVSDTLFSSSGNAFREAVLVQYPRQGSWTIAFVTGKPSGETALHLHGEYLSVY
                     VPTTPNPTSGFFLMVPRADVVVLQMSVDEALKYVISMGVVAPPLHVVPAAPAPAITAA
                     APSAEPARN"
     gene            889..2682
                     /gene="aspS"
                     /locus_tag="VFA35_02260"
     CDS             889..2682
                     /gene="aspS"
                     /locus_tag="VFA35_02260"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZT55022.1"
                     /translation="MRTTYCGLVSETLMGQTVTLMGWAHRRRDHGGVIFIDLRDREGL
                     VQIVCDPDRAEMFAIAEEVRNEFCLKIVGKVRARPEGTVNPNITSGKVEVLCLEIEVL
                     NESITPPFQLDDDNLSETTRLTHRVLDLRRPPMQRNMMLRYRVAMEVRKFLDANGFID
                     IETPMLTKSTPEGARDYLVPSRVHDGMFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDIETSFLTEEEIRTMFEGMIRSTFKHAIGVELPAFPVMKYADA
                     MHRFGSDKPDLRVKLEFTELTDVMKDVDFKVFSGPALMDGGRVVALRVPGGAEMSRSE
                     IDAYTEFVKIYGAKGLAWIKVNDVAKGREGLQSPVVKNLHDAALAEILKRTGAQDGDL
                     LFFGADKAKVVNDAIGALRVKIGHSDFGKARGLAQGAWEPLWVVDFPMFEYDDGAQRW
                     SAVHHPFTSPKDGHEDLLETDPGAAVSKAYDVVLNGIELGGGSVRIHREEVQSKVFRA
                     LKINAEEAQAKFGFLLDALQYGAPPHGGIAIGLDRFVMLMTGAESLRDVIAFPKTQRA
                     QDLLTNAPSAVDEKQLRELHIRLRNVQGAGG"
BASE COUNT          465 a          921 c          924 g          416 t
ORIGIN      
        1 cggccgcgcc ggccccggcc gcgccggcgt ccgagagcaa gtgagcgcgc ggtcgcgcca
       61 ggaaacctcg tgaagaaata cctcatcgcc gggctgctcg tctggctgcc gatggccgtc
      121 accatctggg tgctgcaggc cgcactcggt ctgcttgacg gcgtgttcgg ctggctcctg
      181 aacgccggcc gcgcaatcct gccggcggcg gccgaaccgc tgatcgatct gctgctgaag
      241 atccccggcc tgggcgtgct ggtgatgctg ctcggcttgc tgctgaccgg cgtgttcgcg
      301 accaacatgg ccggccagtg gtttctgcgc cagggccacc ggctgctgaa caagatcccg
      361 atcgtcaagt cgatctacag ctcggtgaag caggtctcgg acacgttgtt ctcgagcagc
      421 ggcaacgcct ttcgcgaggc ggtgctggtg cagtacccgc gccagggcag ttggacgatt
      481 gcgttcgtca ccggcaagcc cagcggcgag accgcgctgc acctgcatgg cgagtacttg
      541 agcgtgtacg tgccgaccac gccgaacccg acctcgggct tcttcctgat ggtgccgcgc
      601 gccgacgtgg tcgtgctgca gatgagcgtc gacgaagcgc tgaagtacgt gatctcgatg
      661 ggcgtggtcg cgccgccgtt gcacgtcgtg ccggcggcac cggcgccggc gatcaccgct
      721 gccgccccga gcgccgagcc ggcccgaaat tgaccgggcc gggcggcccg gcgtccgctc
      781 ggtccacggg cgctgcgccg cagcgccgca gtgccacccc ttcgaacccg cgcaacgcga
      841 gcgccgcgtt gccgacaacc aatctgccga gtgcacagga gtgacccgat gagaaccacc
      901 tactgcggcc tcgtcagcga aacgctgatg ggccagaccg tgacgctgat gggctgggcg
      961 caccgccgcc gcgaccatgg cggcgtgatc ttcatcgacc tgcgcgatcg cgaggggctg
     1021 gtccagatcg tctgcgaccc cgaccgcgcc gagatgttcg cgatcgccga agaggtgcgc
     1081 aacgagttct gcctgaagat cgtcggcaag gtgcgcgcgc gccccgaggg cacggtcaac
     1141 cccaacatca ccagcggcaa ggtcgaggtg ctgtgcctgg agatcgaggt gctgaacgag
     1201 agcatcacgc cgccgttcca gctcgacgac gacaacctct cggagaccac gcgcctcacg
     1261 caccgcgtgc tcgacctgcg ccgcccgccg atgcagcgca acatgatgct gcgctaccgc
     1321 gtcgcgatgg aggtgcgcaa gttcctcgac gcgaacggct tcatcgacat cgaaacgccg
     1381 atgctgacca agagcacgcc cgaaggcgcg cgcgactatc tcgtgcccag ccgcgtgcac
     1441 gacggcatgt tcttcgcgct gccgcagtcg ccgcagctgt tcaagcaact gctgatggtc
     1501 gccggcttcg accgctacta ccagatcacc aagtgcttcc gcgacgaaga cctgcgcgcc
     1561 gaccgccagc ccgagttcac gcagatcgac atcgagacct cgttcctgac cgaggaagag
     1621 atccgcacga tgttcgaggg gatgatccgc agcaccttca agcacgcgat cggtgtcgag
     1681 ctgccggcgt tcccggtgat gaagtacgcc gacgcgatgc accgcttcgg ctccgacaag
     1741 cccgatctgc gcgtcaagct cgagttcacc gagctgaccg acgtgatgaa ggatgtcgac
     1801 ttcaaggtct tctcgggccc ggcgctgatg gacggcggcc gcgtcgtcgc gttgcgcgtg
     1861 cccggcggcg ccgagatgag ccgcagcgag atcgacgcct acaccgagtt cgtcaagatc
     1921 tacggcgcga aggggctggc gtggatcaag gtgaacgacg tggccaaggg ccgcgaaggg
     1981 ctgcagtcgc cggtcgtgaa gaacctgcac gacgcggcgc tggccgagat cctcaagcgc
     2041 accggcgcgc aagacggcga cctgctgttc ttcggcgcgg acaaggccaa ggtcgtcaac
     2101 gacgcgatcg gcgcgctgcg cgtgaagatc ggtcacagcg acttcggcaa ggcacgcggc
     2161 ctcgcgcagg gtgcctggga gccgctgtgg gtggtcgact tcccgatgtt cgagtacgac
     2221 gacggcgcgc agcgctggag cgcggtgcac cacccgttca cgtcgccgaa ggacggccac
     2281 gaggatctgc tcgagaccga ccccggcgcg gccgtctcga aggcctacga cgtggtgctc
     2341 aacggcatcg aactcggcgg cggctcggtc cgtatccacc gcgaggaagt gcagagcaag
     2401 gtgttccgcg cgctcaagat caacgccgag gaggcccagg ccaagttcgg cttcctcctc
     2461 gacgcgctgc agtacggtgc gccgccgcac ggcggcatcg cgatcgggct cgaccgcttc
     2521 gtcatgctga tgaccggcgc cgagtcgctg cgcgacgtga tcgcgttccc gaagacccag
     2581 cgcgcgcagg acctgctgac caacgcgccg agcgcggtcg acgagaagca gttgcgcgag
     2641 ctgcacatcc ggctgcgcaa cgtgcagggg gcgggcggct gattggctag aatggctact
     2701 gttgtggatc gggagcagcc atgaac
//
LOCUS       DASRDG010000138         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_135256, whole genome shotgun sequence.
ACCESSION   DASRDG010000138 DASRDG010000000
VERSION     DASRDG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135256"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFA35_02265"
     CDS             complement(<1..462)
                     /locus_tag="VFA35_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55023.1"
                     /translation="MNLYDRFIGRWEAEAQAFLPDGSSRRHYWQIRFERVLEGRAIQD
                     VWITPPRDGPNVGKSQRWGPFDNQYGMTLRVYDAKLDAWRVTWIDPCADYRAELVGRA
                     RGDEIFQEGRGSDGAALRWIFSDLRAGLFRWRAEVSRDEGAHWHKAIELFAH"
     gene            complement(472..1347)
                     /locus_tag="VFA35_02270"
     CDS             complement(472..1347)
                     /locus_tag="VFA35_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZT55024.1"
                     /translation="MHAPPCDNGRMITRQTVHRSSSVQVHFVRCRPHEGACGPEEHAT
                     ADLLVFPLRGVFVTHYASVGRVVADACHALHFAANRPYRVSHPVEGGDECLVLEPTPE
                     ALAELRAAERDCVQLDAPLIAQSRMLWHRLRLRTASALETEERALHLFARTVRAPMSR
                     PPKSQLRSRGAEIVEATRVTLAAHPGEPWSLTALAQRVHSSPWHLARTFRELAGLPLH
                     RYQLRARMAAALDEVLDSGRDLTTIGIALGFSSHSHFTACFRASFGVTPSALRRGART
                     QQIRKILTAEAAARP"
     gene            1493..1840
                     /locus_tag="VFA35_02275"
     CDS             1493..1840
                     /locus_tag="VFA35_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZT55025.1"
                     /translation="MQYMLMIYSNESRWTKMTQTEREQGVAAYSAYTEALKKAGVLAG
                     ANRLQPTSAATTVRIANGKSQVLNGPYADAKEQLGGYYMIDVPDLDAALSWAARCPGA
                     SYGAIEVRPIWAM"
     gene            1843..2187
                     /locus_tag="VFA35_02280"
     CDS             1843..2187
                     /locus_tag="VFA35_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZT55026.1"
                     /translation="MQYLLLMYQNEAAMRNQPQDKARQVHGAYMAYLDALKKAGVLVA
                     NTGLQPTAAAATVRAPAGKAMVLNGPFAETREQLAGYFLIEVPDLDAALFWAERNPTA
                     SHGAIEVRPVWA"
     gene            2178..>2376
                     /locus_tag="VFA35_02285"
     CDS             2178..>2376
                     /locus_tag="VFA35_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZT55027.1"
                     /translation="MGVVAQTAEAVARRSYGKLVAFLAARTRDVAAAEDALSEAFAAA
                     LVEWPASGIPRTPEAWLLTVAR"
BASE COUNT          389 a          792 c          841 g          354 t
ORIGIN      
        1 gtgcgcgaac agctcgatcg ccttgtgcca gtgcgcgcct tcgtcgcgcg acacctcggc
       61 gcgccagcga aacaagccgg cccgcaggtc gctgaagatc cagcgcagcg ccgcgccgtc
      121 gctgcccctg ccctcctgga agatctcgtc gccgcgcgcc cggccgacaa gctcggcgcg
      181 gtaatcggcg catgggtcga tccacgttac gcgccatgcg tcgagcttcg cgtcgtaaac
      241 gcgcagcgtc atgccgtact ggttgtcgaa gggcccccag cgctgcgact tgccgacgtt
      301 cggcccgtcg cggggcgggg tgatccagac gtcctggatg gcgcggcctt cgagcacgcg
      361 ctcgaagcgg atctgccagt aatggcgccg cgagctgccg tcggggagga aggcctgcgc
      421 ttcggcttcc cagcggccga tgaatcggtc gtagaggttc atgccccgag cttaggggcg
      481 cgccgcggcc tcggctgtca gaatcttgcg gatctgctgc gtgcgcgcgc cgcgccgcag
      541 cgcggagggc gtgacgccga agctcgcgcg gaagcaggcg gtgaagtggc tgtggctcga
      601 gaagccgagc gcgatgccga tcgtcgtcag atcccggccg gaatcgagca cttcgtcgag
      661 cgccgccgcc atgcgcgcgc gcagctggta gcgatgcagc ggcaagccgg cgagctcgcg
      721 gaacgtgcgc gcgagatgcc aaggcgagct gtgcacgcgc tgcgccagtg cggtaaggct
      781 ccacggctca cccggatgtg cagcaagcgt gacccgggtc gcttcgacga tttcggcgcc
      841 gcgcgaccgg agctgcgatt tcggcgggcg ggacatcggc gcgcgcacag tgcgggcgaa
      901 aagatgcagc gcgcgctcct ctgtttccag ggcggacgcg gtgcgcaacc gcagccgatg
      961 ccagagcatg cggctctgcg cgatgagcgg ggcgtcgagt tgcacgcagt cgcgttcggc
     1021 cgcgcgcagc tcggcgagcg cttcgggcgt gggctcgagc accaggcatt cgtcgccgcc
     1081 ctcgaccgga tggctgacgc ggtagggacg gttggccgcg aaatgcagcg catggcaggc
     1141 atcggccacc acgcggccta cgctggcgta gtgcgtgacg aacacgccgc gcagcggaaa
     1201 gacgagcagg tcggccgtcg cgtgctcttc cggcccgcag gcgccttcgt gcggacggca
     1261 acgcacgaag tgcacctgga cgctcgaact tcgatgcacg gtctggcggg taatcatgcg
     1321 cccattgtcg cagggcggcg catgcattcc ggccgcgcat gacttgccgg acaaaccatc
     1381 gcttgacgct tgaatggagt aaattttcgg acgactgtcg aaagcgggcc acctcgcgcg
     1441 tcattgtcat ggcggcgcgt ggcgccgccc atgaccacga cggagacctg acatgcaata
     1501 catgctgatg atctactcga acgagagccg ctggaccaag atgacgcaaa ccgaacgcga
     1561 gcagggagtg gctgcctata gcgcgtatac cgaggcgctg aagaaggcgg gtgtcctggc
     1621 gggcgcgaac cgcctccagc cgacctcggc cgcgacgacc gtgcgcatcg cgaacggcaa
     1681 gtcgcaggtg ctcaatggcc cctatgcgga cgcaaaggag cagctcggcg gttattacat
     1741 gatcgacgtg cccgacctcg acgcagcatt atcctgggcc gcgcgctgcc ccggcgcgag
     1801 ctacggcgcg atcgaagtgc gtccaatctg ggcgatgtag acatgcaata tctcctcttg
     1861 atgtaccaga acgaagccgc catgcggaac cagcctcagg acaaggcgcg ccaggtgcac
     1921 ggcgcctaca tggcctatct ggacgcgctg aagaaggccg gggtgctggt cgcgaacacc
     1981 ggcctgcagc cgaccgcggc cgcggcgacg gtgcgcgcgc cggccggcaa ggcgatggtg
     2041 ctgaacggcc cctttgccga aacccgggag cagctcgcgg gctactttct gatcgaagtg
     2101 cccgacctcg atgccgcgct cttctgggcc gagcgcaatc ccaccgcgtc tcacggcgcg
     2161 atcgaagtgc gtcccgtctg ggcgtagtcg cgcagactgc cgaggccgta gcgcggcgca
     2221 gctacggcaa gctcgtcgct ttccttgccg cgcgcacgcg cgacgtggcc gccgccgagg
     2281 acgcgctgtc cgaggctttt gcggcggcgc tcgtcgaatg gccggcgagc ggcatcccgc
     2341 gcacgccgga ggcctggctg ctcacggtgg cacggc
//
LOCUS       DASRDG010000139         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_723417, whole genome shotgun sequence.
ACCESSION   DASRDG010000139 DASRDG010000000
VERSION     DASRDG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723417"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /locus_tag="VFA35_02290"
     CDS             complement(<1..1624)
                     /locus_tag="VFA35_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HZT55028.1"
                     /translation="MGPAPTTLGWGARIALVSGDGIDGLRDGPAAQARWSDPWGVAID
                     THGTVYVADAGANNRIRRIATDGTVSTLAGGHEGFADGIGAAAAFNTPSGLALDKAGN
                     LYVADTGNHAIRKITPQGTVTTLAGTGTADWRDGPAAQAQFDGPIGVAVDEAGNVYVA
                     DTYNDRIRVIGTDGIVATLAGGNAPGYGDGLGAEASFDTPCAVAIDKSGTLWVADTGN
                     GAIRRVARDGKVDTFARATMLGDKVLLRRPLSLALTHDGHLYVGDMAHGRVLQFAPDG
                     QIAVLTGAGGDGDRFVRPAGIALDAAGALRLSDAASHRLHEIRPAAAAPSVQSPQPIG
                     PAPDAPPPDTGGRWPVRPQNVRHEVVGTVGEARGAYHGDALDHLHDGLDIHNNVGAVV
                     LAIADAKIEDPLPAWGIGELREGLSLDVLSYIHMRVGRNIKDEALDPARFQLLRDEAG
                     ALERVRVRRGTRFNAGDVLGTTNAMAHVHLTWGEGGYTRNAIALGFKDFADHVPPRID
                     SVQIEDADGKVLAQKRRGRLVVPRNARGVAIVV"
     gene            complement(1775..>2744)
                     /locus_tag="VFA35_02295"
     CDS             complement(1775..>2744)
                     /locus_tag="VFA35_02295"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZT55029.1"
                     /translation="FLIARRSLERWGDLSALLAPQFYLQRASRILPTLLLVVALLSLF
                     HLAGLPYYVIDKPNQSLGGAFFAALGFHVNWYEGTTGYLPGGWNVMWSLAVEEVFYLA
                     FPLVCLTLGKVRWMFVAVLAALVLSMPFVRMALHAAGGGIWYEEAYLPGMSAIALGVL
                     AAMLATRVATPPCWLAPILCALGGAGLCAIFFAGAPLWKAIGEYNVWLLVGSAASLVL
                     GLHWQATSARPWRLRGLGWLRSCGRLSYEIYLFHMFVVFALVAVFRGGGFDMHWGFLW
                     YMPTLALAWLLGLAVARGFSQPCERWLRQRFGAAAMKPLLAQPATG"
BASE COUNT          443 a          932 c          982 g          387 t
ORIGIN      
        1 cgaccacgat cgcgacgccg cgcgcgttgc gcggtacgac cagtcgcccg cgacgcttct
       61 gcgcgagcac cttgccgtca gcgtcctcga tctgcacgct gtcgatgcgc ggcggcacat
      121 ggtcggcgaa gtccttgaag ccgagcgcga tcgcgttgcg cgtgtatccc ccctcgcccc
      181 aggtcaggtg cacgtgtgcc atcgcgttgg tcgtgccgag cacgtctccg gcgttgaaac
      241 gcgtgccgcg gcgtacgcgt acgcgctcga gtgcgccggc ctcatcgcgc agcagctgga
      301 agcgtgccgg atccaatgct tcatccttga tgttgcggcc gacgcgcatg tggatgtagc
      361 tcagcacgtc gagactgagg ccctcgcgca gctcgccgat gccccaggca ggcagcgggt
      421 cttcgatctt ggcatcggcg atcgcgagca ccacggcgcc gacgttgttg tggatgtcga
      481 ggccgtcatg cagatggtcg agcgcgtcgc cgtggtaagc gccgcgcgct tcgccgaccg
      541 tgccgacaac ttcgtggcgc acattctgcg gccgcaccgg ccagcgcccg cccgtgtcgg
      601 gcggcggcgc atcgggcgcg ggaccgatcg gctgcggcga ttgcacgctc ggcgcggccg
      661 ctgccggacg aatctcgtgc aggcgatggc ttgcggcgtc gctcaggcgc agcgcgccgg
      721 ctgcgtcgag cgcgatgccg gcgggccgga cgaagcggtc gccgtcgccc cccgcgccgg
      781 tcaacactgc gatctgcccg tcgggcgcga actgcagtac ccgaccgtgc gccatgtcgc
      841 cgacgtagag atgcccgtcg tgggtcagcg cgaggctcag cggccggcgc agcagcacct
      901 tgtcgccgag catggtcgcg cgcgcgaacg tgtcgacctt gccgtcgcgc gcgacgcggc
      961 ggatcgcgcc gttccccgta tcggccaccc agagcgtacc cgatttgtcg atcgcgacag
     1021 cgcacggcgt atcgaagctc gcctcggcac cgaggccgtc gccatagccc ggcgcgttgc
     1081 cgccggccag ggtggcgacg atgccgtcgg taccgatcac gcggatgcgg tcgttgtagg
     1141 tgtcggcgac atagacgttg ccagcttcgt cgaccgcgac gccgatcggc ccatcgaact
     1201 gcgcttgcgc ggccggtccg tcgcgccagt cggctgttcc cgtgccggcc agtgtcgtca
     1261 ccgtgccttg cggcgtgatc ttgcggatcg cgtggttgcc cgtgtcggcg acgtagagat
     1321 tgccggcctt gtcgagggcg aggcccgagg gcgtgttgaa tgccgctgcc gcgccgatgc
     1381 cgtcggcgaa gccttcgtgg ccgccagcca aggtcgagac cgtgccatcg gtcgcgatgc
     1441 ggcggatgcg gttgttggcg cccgcatcgg cgacgtagac cgtgccgtgc gtatcgatcg
     1501 cgacacccca gggatcggac cagcgcgcct gtgcggccgg gccatcgcgc aggccgtcga
     1561 tgccgtcacc ggagacgaga gcgatgcgtg cgccccagcc gagcgtcgtt ggcgcggggc
     1621 ccaccggcgt cggcgcgtgt gccggagatg ccggctgcga cgtcggtctg ttctgatggg
     1681 caagtataaa aatggacaat gcggcaacgc ccgcggcgca aagcaggagc aggccgcgcc
     1741 acggcgggcg agatcgaagc gtcatgatgg aggatcagcc cgtcgcgggc tgcgcgagga
     1801 gcggcttcat cgctgcggct ccgaaacgtt gtcgcagcca gcgctcgcac ggttgcgaga
     1861 agccgcgcgc gactgcgagg ccgagcagcc aggccagcgc gagcgttggc atgtaccaga
     1921 gaaagcccca gtgcatgtcg aatccgccgc cgcgaaagac cgcgacgagc gcgaacacga
     1981 cgaacatgtg gaacaggtag atctcgtaac tgagccggcc gcaggaacgc agccagccga
     2041 ggccgcgcag ccgccacggt cgcgccgagg tcgcctgcca gtgcaggccg aggacgaggc
     2101 ttgcggcgct gccgaccagc agccagacgt tgtattcgcc gattgctttc cacagcggcg
     2161 cgccggcgaa gaagatggcg cagaggccgg cgccgccgag cgcgcacagg atcggcgcga
     2221 gccagcacgg cggcgtggcg acgcgcgtgg cgagcattgc cgcgagcacg ccgagggcga
     2281 tcgcggacat gcccggcaga tacgcttcct cgtaccagat gccgccgccc gccgcgtgca
     2341 gcgccatgcg cacgaacggc atcgacagca cgagcgcggc cagcacggcg acgaacatcc
     2401 agcgcacctt gccgagggtg aggcagacca gcgggaacgc gagatagaac acttcctcaa
     2461 cggccagcga ccacatcacg ttccagccgc cgggcagata gcccgtggtg ccctcatacc
     2521 agttcacgtg gaatccgagc gcggcgaaga acgcgccgcc caacgactgg ttcggcttgt
     2581 cgatcacgta gtagggcaag ccggcgagat ggaacaggct cagcagcgcg acgacgagca
     2641 gcagcgtcgg caggatgcgc gaggcgcgtt gcagatagaa ctgcggcgcc aacaacgcgc
     2701 tgagatcacc ccagcgttcg agcgagcggc gcgcgatcag gaag
//
LOCUS       DASRDG010000140         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_254895, whole genome shotgun sequence.
ACCESSION   DASRDG010000140 DASRDG010000000
VERSION     DASRDG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254895"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="VFA35_02300"
     CDS             complement(<1..1623)
                     /locus_tag="VFA35_02300"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HZT55030.1"
                     /translation="MQTRTVPTQAIAGQRPGTSGLRKKVTVFQQPPYLENFVQSIFDS
                     LDGFAGRTLVVGGDGRFFNDVAIQTILRMAAANGFGKVLVGQHGILSTPAASCVIRKH
                     AAFGGIILSASHNPGGPDGDFGIKYNTGNGGPAPEKITEAIFANTEKITRYLTVDAAD
                     VDLGKLGTSTVAGMAVEVIDSVADHAELLASLFDFERIGALLRSGSFRMCFDAMHAVT
                     GPYAQEIFVKRLGAPAASVINSVALPDFGGGHPDPNPTYAEDLMARMFAEGSGSESAP
                     DFGAASDGDGDRNMIVGRRFVVTPSDSLAVLAANATLVPGYAGGLKGLARSMPTSAAV
                     DRVAEKLGIACFETPTGWKFFGNLLDAGQITLCGEESAGTGSDHVREKDGVWAVLFWL
                     NILAVRGESVEAIVRDHWKTYGRNYYSRHDYEGVDSAAANALIAHLRESLATLPGRTV
                     AGATIASADDFAYTDPVDGSVSTAQGIRIGLADGSRIVFRLSGTGTEGATLRVYLERF
                     EPDAARHAIETQAALASLIALADALAGIRERTG"
     gene            complement(1724..>2195)
                     /locus_tag="VFA35_02305"
     CDS             complement(1724..>2195)
                     /locus_tag="VFA35_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZT55031.1"
                     /translation="LDAIKLSTTNCADAACAQAAGAIGVFDFVAGVVYNPGTQRAPAD
                     AKLFGTGPTGTTVNNTVATLGLFDAVSSTGPDAAGFLSLGDNGSIAFNLTSAISTAGL
                     YLYIGEVGDNGEVAGSNIELRHDPVPGIPEPSTYALMLAGLGAVGWLARRQRRG"
BASE COUNT          304 a          758 c          795 g          338 t
ORIGIN      
        1 cccggtacgc tcgcggatgc ctgcgagcgc atcggccagc gcgatcagcg acgccagcgc
       61 ggcctgcgtt tcgatcgcat gccgggcggc gtcgggctcg aagcgttcga ggtagacgcg
      121 cagcgtcgcg ccctcggtgc cggtgcccga gagccggaag acgatgcgcg agccgtcggc
      181 caggccgatg cggatgcctt gcgcggtgct gaccgagccg tcgaccgggt cggtgtaggc
      241 gaagtcgtcg gcgctggcga tcgtcgcgcc ggcgaccgtg cggcccggca gcgtcgcgag
      301 cgactcgcgc aggtgcgcga tcagggcgtt ggccgccgcg ctgtcgacgc cttcgtagtc
      361 gtggcgcgag tagtagttgc ggccgtaggt cttccagtga tcgcgcacga tcgcctcgac
      421 cgactcgccg cgcaccgcga ggatgttgag ccagaacagc acggcccaga cgccgtcctt
      481 ctcgcgcacg tggtcggagc cggtgccggc gctctcttcg ccgcacagcg tgatctggcc
      541 ggcgtcgagc aggttgccga agaacttcca gccggtcggc gtctcgaagc acgcgatgcc
      601 gagcttctcg gcgacacgat cgaccgccgc gctcgtcggc atcgagcgcg cgaggccctt
      661 cagcccgccc gcgtagcccg gcaccagcgt cgcgttcgcg gccagcaccg cgaggctgtc
      721 gctcggcgtg acgacgaagc gccggccgac gatcatgttg cggtcgccgt cgccgtcgct
      781 cgcggcgccg aaatcgggcg cactctccga tcccgatcct tcggcgaaca tgcgcgccat
      841 caggtcctcg gcgtaggtcg gattcgggtc ggggtgcccg ccgccgaagt cgggcagcgc
      901 aacagagttg atcacgctcg ccgccggcgc gccgaggcgc ttgacgaaga tctcctgcgc
      961 atacgggccg gtgaccgcgt gcatcgcgtc gaagcacatg cggaagctgc ccgagcgcag
     1021 cagcgcgccg atgcgctcga agtcgaacaa cgaggcgagc agttcggcgt ggtcggcgac
     1081 cgagtcgatc acctcgaccg ccatgccggc aaccgtgctc gtgccgagct tgccgaggtc
     1141 gacatcggcg gcgtcgaccg tcaggtagcg ggtgatcttc tcggtgttcg cgaagatcgc
     1201 ctcggtgatc ttctcgggcg ccgggccgcc gttgccggtg ttgtacttga tgccgaagtc
     1261 gccgtcgggc ccgcccgggt tgtggctcgc cgacaggatg atgccgccga acgcggcgtg
     1321 cttgcggatc acgcaactcg ccgccggcgt cgacaggatg ccgtgctgcc cgaccagcac
     1381 cttgccgaag ccgttggcgg cggccatgcg caggatggtc tggatcgcca cgtcgttgaa
     1441 gaagcggcca tcaccgccga cgaccagcgt gcggcccgcg aagccgtcga gcgagtcgaa
     1501 gatcgactgc acgaagttct cgaggtacgg cggctgctgg aacaccgtga ccttcttgcg
     1561 caggccggag gtgcccgggc gctgcccggc gatcgcttgg gtcggaacgg tacgggtctg
     1621 catgcttgcc tccagttctt ggtctggctg cattgtgccg cgatgccggc gcgctgcgca
     1681 cgcgctcgcc cccgccgcgg cggcgcgctg ctcccggcgt gcgtcagccg cgccgctggc
     1741 gccgcgccag ccagccgacc gcgccgaggc cggccagcat caaggcgtag gtcgaaggct
     1801 cggggatgcc cggcaccgga tcgtgccgca actcgatgtt ggagccggcg acttcgccgt
     1861 tgtcgccgac ctcgccgatg tacaggtaca ggcccgcggt ggagatcgcc gacgtgaggt
     1921 tgaacgcgat gctgccgttg tcgcccaggc tcagaaagcc cgccgcatcc gggccggtgc
     1981 tcgacacggc atcgaacagc cccagcgtcg cgaccgtgtt gttgaccgtc gtgccggtcg
     2041 ggccggtgcc gaagagcttg gcgtcggccg gtgcgcgctg cgtgcccggg ttgtagacca
     2101 cgccggcgac gaagtcgaac acgccgatcg cgccggcggc ctgcgcgcag gccgcgtcgg
     2161 cgcagttggt cgtcgacagc ttgatcgcgt cgaga
//
LOCUS       DASRDG010000141         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1629907, whole genome shotgun sequence.
ACCESSION   DASRDG010000141 DASRDG010000000
VERSION     DASRDG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629907"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFA35_02310"
     CDS             complement(<1..335)
                     /locus_tag="VFA35_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55032.1"
                     /translation="MTSNDMRAALANDDGSLPERWNPDDVPGTMLIGTLLRFDSIVTK
                     IGPGSIAVIEDDDGTEWGVLLGRAVLKKRFETLDPKPGDSVGLKYVGYVEPRNKDSQG
                     YHNYVMRVQR"
     gene            complement(335..547)
                     /locus_tag="VFA35_02315"
     CDS             complement(335..547)
                     /locus_tag="VFA35_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55033.1"
                     /translation="MKEHEYMIVRTWHTRKRRHQIAVGPYPSRYRAQRKIAEYVNEWG
                     YWNDRDQFTVALYRHPAETERLQEVV"
     gene            complement(594..965)
                     /locus_tag="VFA35_02320"
     CDS             complement(594..965)
                     /locus_tag="VFA35_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55034.1"
                     /translation="MALSRGRSLLLRAATENEFAGWVKHVAYIHGWNGIHIRWSQGVL
                     ESVHTLRRDGWSEAYGLPDWLFWSEEREQSFWAELKSWSGRVSREQKRQIPSMRTGGM
                     TVFLWKPQDEEEIERVFRDGL"
     gene            complement(1075..2124)
                     /locus_tag="VFA35_02325"
     CDS             complement(1075..2124)
                     /locus_tag="VFA35_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55035.1"
                     /translation="MTATVDRSAVPRMTMSDLDRLRDWYGRMGSPRPHAVDIDRLYEA
                     MFEKLKKDPILVRYIHHIGTANHAAHNIEPWTALGEQRYAAFECLGSALPESGLKIGH
                     GNASVQAIRGERSSREFALILATRCLMAAPYLWTNEVRELVKESPLPPHVISRNVLPH
                     PAVFFSSEDSLSVADAHGEDTEVETNWELLFDEGEAGFTVTLDLFRNQSDVTLATSFV
                     PYGRKFPDDIPAGAMRNATEHLLQQLAFIRSPYTVTEPTRLPRPMRRELQRAKAKDVD
                     PLVYVVKLRRAVERKPPPEESVEVNWQHHWWVSGHYRAQWHPSTKSHEVIWIGPHVKG
                     PLDKPLLEKVYAVVR"
     gene            complement(2121..>2541)
                     /locus_tag="VFA35_02330"
     CDS             complement(2121..>2541)
                     /locus_tag="VFA35_02330"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HZT55036.1"
                     /translation="AGIHAQADDVAEAAAAAGLDRQEVAGAVNSQPGVNVWQYLRWEG
                     KLAPLPSPKLEVLTGTVWDTLAMCESTSTWNANTGNGYYGGIQFDLPTWRAYGGLAYA
                     ARPDLASRAAQIAVAEKLRAVRGFQPWPVCSRRLGLR"
BASE COUNT          469 a          853 c          732 g          487 t
ORIGIN      
        1 cgctgcactc gcatcacata gttgtggtag ccctgagaat ccttgttgcg gggctccaca
       61 taaccgacgt acttcagtcc cactgaatct ccaggtttag ggtccagggt ttcaaagcgc
      121 ttcttcagca ccgcgcgccc aagcaagacg ccccactcgg tgccgtcgtc gtcctcgatg
      181 actgcaatgc tgccggggcc gatcttggtc acgatcgaat cgaagcgcag aagcgtgccg
      241 atcagcatcg tgcccggcac gtcgtcgggg ttccagcgct caggcagtga accatcgtca
      301 ttggccagcg cggcacgcat atcgtttgat gtcatcacac cacctcctgc aggcgttcag
      361 tctccgccgg atggcggtac agcgctaccg tgaactggtc acggtcattc cagtaacccc
      421 actcattgac atactccgca attttccgct gggcccggta gcggctcggg tagggaccga
      481 ccgcaatctg atgcctgcgc tttctggtgt gccaggtgcg cacaatcatg tactcgtgtt
      541 ctttcaccct tgcgcgttct cctgcacgcg aacaaggcgc gcgtgcccgc cacttagaga
      601 ccatctcgaa agacgcgctc aatctcctcc tcgtcttgtg gtttccacaa aaagacagtc
      661 atgccaccgg tgcgcatgga cggaatctgg cgcttctgtt cgcgcgagac gcgaccactc
      721 caggacttca gctcagccca gaagctttgc tcgcgctcct ccgaccagaa caaccaatcc
      781 ggcaagccgt acgcctccga ccaaccgtct cgtcgcagcg tgtgcacgct ctccagcacg
      841 ccctgcgacc agcgaatgtg gatgccgttc cagccatgga tgtacgcgac atgcttgacc
      901 cagcctgcga actcgttctc ggtcgccgcg cgcagtaaca gactgcggcc tctgctcagc
      961 gccactaccg ataacgctcg acttcttcgc gcgtggccag cagcaggccc atgacgaagg
     1021 cgcccacgca gcaccccaca acagccccga gcaggaacgc cagcacgtat tccatcagcg
     1081 caccaccgcg tacaccttct cgagtaatgg cttgtcgagc ggacctttca cgtgcggccc
     1141 aatccagatg acctcatgcg acttggtgct ggggtgccac tgtgcgcggt agtgaccgct
     1201 cacccaccag tggtgctgcc agttgacctc gacggattcc tccggcggcg gcttgcgctc
     1261 gactgcgcgc cgcagcttga ccacatacac cagcggatca acatcctttg cctttgcccg
     1321 ctggagttcg cggcgcattg gtcgcgggag ccgcgtaggc tccgtcaccg tgtagggcga
     1381 gcggataaaa gcgagctgct gcaagagatg ctcggtagca ttgcgcatcg cgccggcagg
     1441 gatgtcgtct ggaaacttgc ggccgtacgg cacaaatgat gttgccagcg tgacgtcaga
     1501 ctggtttcgg aacaagtcca gggtgaccgt gaaacctgcc tcaccctcat cgaagagcaa
     1561 ctcccagttc gtctcaacct cggtgtcctc accatgagca tccgccaccg acagggagtc
     1621 ttccgacgag aagaagaccg cggggtgagg taacacattg cgactgatga cgtgcggcgg
     1681 cagcggcgac tccttgacca gttcgcggac ctcgttggtc cacagatacg gtgccgccat
     1741 cagacagcgt gtcgcgagaa tcagcgcaaa ctcacggcta gacctctcac cgcggatggc
     1801 ctgcacgctg gcgttgccat gccctatctt cagcccgctc tcaggtagag ctgaccccag
     1861 acactcaaac gcggcatagc gctgctcgcc gagcgctgtc cacggttcaa tgttgtgcgc
     1921 ggcatggttt gccgtgccga tgtggtggat atagcgcacc aggatcgggt cttttttgag
     1981 cttctcgaac atggcctcgt acaggcgatc gatgtcaacc gcatgaggcc gcgggctgcc
     2041 catgcggcca taccaatcac gcaacctgtc caggtcggac atagtcatac gcggtaccgc
     2101 gctgcggtcg acggtggctg tcatcgcaat cccaatcgcc ggctgcacac cggccagggt
     2161 tggaatccgc gtacggcgcg gagcttctcg gcgacagcga tctgcgctgc tctgctggcc
     2221 aggtcgggcc gagctgcgta ggcgagcccg ccgtacgcac gccaggttgg cagatcaaat
     2281 tgaatgccgc cgtagtagcc attgcctgtg ttcgcgttcc aggtggacgt cgactcgcac
     2341 atggccagcg tgtcccacac ggtcccggtc agcacctcga gcttcggcga cggcagcggc
     2401 gcgagcttgc cctcccagcg caggtactgc cagacgttca cacccggctg cgagttgacc
     2461 gctccggcta cctcctgccg gtccaggccg gccgccgctg cagcctcggc gacgtcgtca
     2521 gcctgggcat ggatgccggc c
//
LOCUS       DASRDG010000142         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1629966, whole genome shotgun sequence.
ACCESSION   DASRDG010000142 DASRDG010000000
VERSION     DASRDG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629966"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..>2088
                     /locus_tag="VFA35_02335"
     CDS             19..>2088
                     /locus_tag="VFA35_02335"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZT55037.1"
                     /translation="MFVGAVLGGWLSWIVFDESVGAVGMAFGIICGLLFGRLAKLGKR
                     VGQLEAQLKALVSAPAVQRPETPVVAAPPTAPTVPVETAAALSAVTPAQAVFATTTTG
                     ADPGATTIDLRKPAAAVEAQPPPRIPTPPRADPPALAWIKRWLTEGNPLVKAGVVVVF
                     FGVAYLFKYAADAGWLTVPVEFRFLGVAVAALAALAFAWRKRESHRSFALSLQGGAIG
                     VLILTIFAAYRLYSLLPPTLTFALLVIVVAGAGLLAVLQDSPALAVLAVLCGFLAPIL
                     ASNDSGNHVALFSYYAVLNAAIFFIAWIKPWRALNLVGFVFTFGVGAAWGALKYRPEL
                     IASTEPFLLLFFAFYLLIPLLHARQQARNESDSGSNRVQAVVSGSLVFGTPLVVFALQ
                     AALLEGARMPLAFSALGAAAIYAALAVLELKHWRLQLLGESHALLALGFATIAVPLAL
                     SAQATMCTFALEGAALVWLGLRQARRIPRWIGYALQGLAGAIFGERFGRIEAMPVLNG
                     EFLGAALLALAGLVSARLLHRRDARAPVALLFLVWGGFWWYFAGGNEIERFVAHARHW
                     DAWLAFLAVSALIAVAAARRLAWRELLVPAAITIALGLPFVGLTMLDDHGPLEGWHLA
                     AWALWLLAAWRTLPALAAEQLRAARILHFVYLWICVLLLALELGHLGHDHLHLRAIWF
                     ALTLLVPF"
BASE COUNT          246 a          755 c          675 g          412 t
ORIGIN      
        1 gcgatgacgt tcttgtggat gttcgtgggc gccgtgctcg gcggctggct gagctggatc
       61 gtgttcgacg aatcggtcgg cgcggtcggc atggcgttcg gcatcatctg tgggctgctg
      121 ttcggccggc ttgccaagct cggcaagcgg gtcggtcaac tcgaggcgca gctcaaggcg
      181 ctggtttcgg cgcctgccgt gcagcggccc gagacaccag tcgtcgcggc cccacccacg
      241 gccccgacgg tgccggtcga aacggccgca gcgctgtctg cggtaacgcc ggcgcaggcc
      301 gttttcgcca caaccaccac cggcgccgat cccggcgcga caacgatcga tttgcgcaaa
      361 cccgcggccg cggttgaagc ccagccgcca ccacgcatcc cgactccgcc gcgcgccgat
      421 ccacccgcgc tcgcctggat caagcgctgg ctgaccgaag gcaatccgct ggtcaaggcc
      481 ggtgtcgtcg tcgtgttctt cggcgtcgcc tatctgttca agtacgcggc cgacgccggc
      541 tggctgaccg tgccggtcga gttccgtttc ctcggcgtcg ccgtcgccgc gctcgccgcg
      601 ctcgcattcg cctggcgcaa gcgcgagtcg catcgcagct tcgcgctgag cctgcagggc
      661 ggcgcgatcg gcgtgctgat cctgaccatc ttcgccgcgt atcgcctcta ctcgctgctg
      721 ccgccgacgc tgaccttcgc cctgctcgtc atcgtcgtcg ccggcgcggg tctgctcgcg
      781 gtgctgcagg attcgcctgc gctcgccgtg ctcgccgtcc tatgcggctt cctcgcaccg
      841 atcctcgcct cgaacgacag cggcaaccac gtcgcgctgt tctcgtacta cgcggtgctc
      901 aacgcggcga tcttcttcat cgcctggatc aagccgtggc gcgcgctcaa cctcgtcggc
      961 ttcgtcttca ccttcggcgt cggcgcggcc tggggcgcgc tcaagtatcg gccggaattg
     1021 atcgcctcga ccgaaccgtt cctgctgctg ttcttcgcgt tctatctgtt gatcccgctg
     1081 ctgcatgcgc ggcaacaggc gcgaaatgag agcgattccg gatcgaaccg cgtgcaggcc
     1141 gtggtcagcg gcagcctcgt gttcggcacg ccgctcgtcg tgttcgcgct gcaggctgcg
     1201 ctgctcgagg gcgcgcgcat gccgctcgct ttcagcgcac tcggcgccgc ggcgatctac
     1261 gccgcgctcg ccgtgctgga actcaaacac tggcgcctgc aactgctcgg cgaatcgcac
     1321 gcgctgctcg cgctcggctt cgccacgatc gcggtgccgc tcgcgctgtc ggcgcaggcg
     1381 acgatgtgca cgttcgcgct tgaaggcgca gcgctggtct ggctcggcct gcgccaggct
     1441 cgacgcattc cgcgctggat cggctacgca ctgcaaggcc tggccggcgc gatcttcggt
     1501 gagcgcttcg gccgcatcga agccatgccc gtcctcaacg gtgaatttct cggcgccgcg
     1561 ctgcttgcgc tggccggcct ggtcagcgcg cgcctgctgc accggcgcga cgcgcgcgca
     1621 ccagtggcct tgctgttcct cgtctggggc ggattctggt ggtacttcgc cggcggcaat
     1681 gaaatcgaac gctttgtcgc gcacgcgcgg cactgggacg catggctcgc ctttctcgcc
     1741 gtcagcgcgc tgatcgccgt agcagccgca cgccgcctgg cctggcgcga attgctcgtg
     1801 ccggcggcaa tcacgatcgc gctcggcctg ccgttcgtcg gcctgaccat gctcgacgac
     1861 cacggcccgc tcgaaggctg gcatctcgcc gcatgggcgc tctggttgct cgccgcgtgg
     1921 cgtacgctgc cggcgctggc cgccgaacag ctgcgcgctg cgcgcattct gcattttgtt
     1981 tatttgtgga tttgtgtact cttgcttgca ctcgagctcg gccatctcgg ccacgaccat
     2041 ctgcacctgc gcgcgatctg gttcgcgctg accctgctcg tccccttt
//
LOCUS       DASRDG010000143         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1405945, whole genome shotgun sequence.
ACCESSION   DASRDG010000143 DASRDG010000000
VERSION     DASRDG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405945"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>2548
                     /locus_tag="VFA35_02340"
     CDS             29..>2548
                     /locus_tag="VFA35_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZT55038.1"
                     /translation="MSTSAIDLRSDQEEVTPQGPPLRFVTAASLFDGHDAAINVMRRI
                     MQASGAEVIHLGHNRSVAEIVECAIQEDVQGIAITSYQGGHVEYFKYMVDLLQARGGA
                     HIQIFGGGGGVIMPSEIAELQRYGVTRIYSPEDGQRMGLAGMIGEMVMRCDVDLSGHA
                     PRALAAIQGQTETHWRALAQLITALENGKVSDELKATMHAEAKLATTPVLGITGTGGA
                     GKSSLTDELIRRLRLDQNDALRIAVISIDPSRRKSGGALLGDRIRMNAIGPWSQGQRV
                     FMRSLATRDFGSEISQALPDVLAAVKASIGFDLIVVETSGIGQGDAAIVPLVDCPVYV
                     MTPEFGAASQLEKIDMLDFAEFVAINKFDRKGAQDALRDVAKQVQRNRQAWTTKADRM
                     PVFGTTASRFNDDGVTALYQALRPRLAALGMPVSAGTLPPIASRHSTHQTPIVPGARV
                     RYLADIADTVRDYKAHARSEARLAREIQQLHAAAWMLADEKPERARAGEATLNLAEQR
                     EARQDPRAKKLLGMWPEMRKAYAGDEYVVKIRDKEIRTALTHTTLSGSKIRKVALPAF
                     EDHGEILQWLMLDNVPGSFPYTAGTFAFKREGEDPTRMFAGEGDAFRTNARFKLLSAG
                     MAAKRLSTAFDSVTLYGNDPALRPDIYGKVGNSGVSIATLDDMKALYDGFDLTDPATS
                     VSMTINGPAPTILAMFMNTAIDQYLDKFRADNGREPSADEAAKISAWVKENVRGTVQA
                     DILKEDQGQNTCIFSTEFSLKVMGDIAEYFVAHKVRNFYSVSISGYHIAEAGANPISQ
                     LAFTLSNGFTFVEAYLARGMAIDDFAPNLSFFFSNGMDPEYT"
BASE COUNT          472 a          895 c          835 g          346 t
ORIGIN      
        1 aagcgactgc gaaggagcag taccggtcat gagcacgtcc gccatcgatc ttcgctccga
       61 tcaggaggag gtcacgccgc agggcccgcc gctgcgcttc gtcaccgcgg cctcgctctt
      121 cgacggccac gacgcggcga tcaacgtcat gcgccggatc atgcaggcgt cgggcgccga
      181 ggtcatccac ctggggcaca accgctcggt cgccgagatc gtcgagtgcg ccatccagga
      241 ggatgtccag ggcatcgcca tcacctcgta ccagggcggc cacgtcgagt acttcaagta
      301 catggtcgac ctgctccaag cacgcggcgg cgcgcacatc caaatcttcg gcggcggcgg
      361 cggcgtgatc atgccgagcg agatcgccga gctgcagcgc tacggcgtca cgcgcatcta
      421 cagccccgaa gacggccagc gcatgggcct ggccggcatg atcggcgaga tggtgatgcg
      481 ctgcgatgtg gacctgtcgg ggcatgcgcc cagggcgctg gccgcgatcc agggccagac
      541 cgagacgcac tggcgcgccc tggcgcagct gatcaccgcg ctcgaaaacg gcaaggtgag
      601 tgacgagctc aaggccacga tgcacgccga ggccaagctg gcgacgaccc ccgtgctcgg
      661 catcaccggc accggcggcg cgggcaagtc gagcctgacc gacgagctga tccgccgcct
      721 gcgcctcgac cagaacgacg cgctgcgcat cgcggtgatc tcgatcgacc cgagccggcg
      781 caagagcggc ggcgcgctgc tcggcgaccg catccggatg aacgcgatcg ggccttggag
      841 ccaagggcaa cgcgtgttca tgcgctcact cgcgacccga gacttcggct ccgaaatcag
      901 ccaggccctg cccgacgtgc tcgccgcagt gaaggcctcg atcggcttcg acctgatcgt
      961 cgtcgagacc tcgggcatcg gccagggcga cgcggcgatc gtgccgctcg tcgactgccc
     1021 ggtgtatgtg atgacgcccg agttcggcgc cgcgagccag ctcgagaaga tcgacatgct
     1081 cgacttcgcc gagttcgtcg cgatcaacaa attcgaccgc aaaggcgcgc aggacgcgct
     1141 gcgcgacgtc gcgaagcagg tgcagcgcaa ccgccaggcg tggacgacga aggccgatcg
     1201 gatgccggtg ttcggcacca cagcgagccg cttcaacgac gacggcgtga ccgcgctgta
     1261 ccaggcgctg cggccgcgcc tggcggcgct cggcatgccg gtcagcgccg gcacgctgcc
     1321 gcccatcgcg agccgccaca gcacgcacca gacgccgatc gtgccgggcg cgcgggtgcg
     1381 ttacctcgcc gacatcgccg ataccgtgcg cgactacaag gcgcacgcac gcagcgaggc
     1441 caggctcgcg cgcgagatcc agcagctgca cgcggcggcc tggatgctcg ccgacgagaa
     1501 gcccgagcgc gcgcgcgccg gcgaggccac gctcaacctc gccgaacaac gcgaagcgcg
     1561 gcaggacccg cgcgccaaga agctgctcgg catgtggccc gagatgcgca aggcgtatgc
     1621 cggtgacgag tacgtcgtga agatccgcga caaggagatc cgcaccgcgc tgacgcacac
     1681 aacgctgtcg ggctcgaaga tccgcaaggt tgcgctgccg gccttcgaag accacggcga
     1741 gatccttcag tggctgatgc tcgacaacgt gcccggcagc ttcccgtaca ccgccggcac
     1801 gttcgcgttc aaacgcgagg gtgaagaccc gacccgcatg ttcgccggcg aaggcgacgc
     1861 cttccgcacc aacgcgcgct tcaagctgct gagtgcgggg atggccgcga agcgcctgtc
     1921 gaccgcgttc gacagcgtca cgctctacgg caatgacccg gcgctgcggc ccgacatcta
     1981 cggcaaggtc ggcaactcgg gcgtgagcat cgccacgctc gacgacatga aggcgctgta
     2041 cgacggcttc gacctgaccg accccgcgac cagcgtgagc atgacgatca acggcccggc
     2101 gccgacgatc ctcgcgatgt tcatgaacac cgcgatcgac cagtacctcg acaagttccg
     2161 cgccgacaac ggccgcgagc ccagcgcgga cgaagccgcg aagatcagcg cgtgggtcaa
     2221 ggagaacgtg cgcggcacgg tgcaggccga catcctgaag gaagaccagg gccagaacac
     2281 ctgcatcttc tcgaccgagt tctcgctcaa ggtgatgggc gacatcgccg agtacttcgt
     2341 cgcccacaag gtgcgcaact tctactcggt gtcgatctcg ggctatcaca tcgccgaggc
     2401 cggcgccaac ccgatcagcc agctcgcgtt cacgctctcg aacggcttca ccttcgtcga
     2461 ggcctacctc gcgcgcggca tggcgatcga cgacttcgcg cccaacctgt cgttcttctt
     2521 ctcgaacggc atggacccgg agtacacg
//
LOCUS       DASRDG010000144         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1285300, whole genome shotgun sequence.
ACCESSION   DASRDG010000144 DASRDG010000000
VERSION     DASRDG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285300"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VFA35_02345"
     CDS             <1..1088
                     /locus_tag="VFA35_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZT55039.1"
                     /translation="RPLVCLLGLPFDAIDIGQAVERIRRAAREGRRCFVSTPNLNFVM
                     TARFDATFRGSVLRSDLSLVDGMPLVWAARLLGLPLPGRVSGADVFEALQDHAGPPVT
                     VFLFGGPPGVAARACEAINRRAGGLRCVGFDEAGYGSVESMSADDRIEKINRSGAQFV
                     IASLGASKGQAWLERNADRLNAPVLSHLGAVLNFAAGSVQRAPRWMAVSGLEWAWRIK
                     EEPALWRRYWRDGIGALWLLLTRVIPDAIATRGPGQRAVPAREPARLQISPAPPGLLL
                     ALSGSWHGHEATLRSALTRCAAEADHLRIDLTGAAELDNAAIALLLIARGWFEARGGF
                     EVVGASRTLSVGLRRKLATAALLGADR"
     gene            1085..1645
                     /locus_tag="VFA35_02350"
     CDS             1085..1645
                     /locus_tag="VFA35_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55040.1"
                     /translation="MNRQDAPELRAEPRISAETPDWSREAKPFWHWSPSRSLLASLRA
                     YERSRLSARPDQVLRRWLAVLRHRFWSAVTGAEIPLGTRIDGGLMMTHPNGIVVHCDA
                     RIGPNCLILQQVTLGVGGARPGAPTLVGHCDIGAGAKLLGGITIGAHAVVGANAVVLC
                     DVPADATAVGIPARVLSARSTKALDG"
     gene            1638..>2585
                     /locus_tag="VFA35_02355"
     CDS             1638..>2585
                     /locus_tag="VFA35_02355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZT55041.1"
                     /translation="MADSLRVLIAAEHASARFGGEAALPLHYFRVMRARGLPVWLVTH
                     ARTRDELSALYPNDPNIVYIEDTAWHRAMWRLGQRLPDAISYVTTGFLSRLSTQWAQR
                     RIVSDMVKRHGIDVVHQPMPVSPREPSMLYGFGVPVVIGPMNGGMDYPPAFRRQRGVA
                     ERWLLGAGRASAALLNCLIPGKRRAATLLVANERTRAALPAGVCTNVVGLVENGVDLG
                     VWRPTPQPEGAAAQDGVTTFVFMGRLVDWKAVDLLLHAFARARDQAPMRLWILGDGSE
                     RARLEGLAGTLGVATRPSAGAGAIEFAGWLPQATCAARLA"
BASE COUNT          320 a          928 c          954 g          383 t
ORIGIN      
        1 cccggccgct ggtgtgcctg ctcgggttgc ccttcgatgc gatcgacatc ggccaggcgg
       61 tggaacggat ccgtcgcgcg gcgcgcgagg ggcgccggtg tttcgtctcg acgcccaacc
      121 tcaatttcgt gatgacggca cgcttcgacg ccaccttccg cggctcggtg ctgcgcagcg
      181 atctaagcct ggtcgacggc atgccgctgg tgtgggccgc gcgcctgctg ggcttgcccc
      241 tccccgggcg cgtctcgggt gccgatgtgt tcgaggcgct ccaggaccac gccggtccgc
      301 ccgtcacggt cttcctgttc ggcgggccgc cgggcgtggc ggcccgcgcc tgcgaggcga
      361 tcaaccgccg cgccggcggc ctgcgatgcg tcggcttcga cgaggccggc tatggcagcg
      421 tcgagtcgat gagcgccgac gatcggatcg agaagatcaa ccgcagcggc gcgcagttcg
      481 tgatcgcctc gctgggggcc agcaaagggc aggcctggct cgagcgcaat gccgaccgct
      541 tgaacgcgcc ggtcctgtcg caccttggcg ctgtcctgaa cttcgcggcc ggcagcgtgc
      601 agcgcgcgcc gcgctggatg gcggtcagcg ggctcgaatg ggcctggcgc atcaaggagg
      661 aaccggcgct gtggcggcgt tactggcgcg atggcatcgg cgcactctgg ctcttgctga
      721 cgcgcgtgat ccccgatgcg atcgccaccc gcgggccggg gcagcgtgcg gtcccggccc
      781 gcgaaccggc gcggttgcag atctcgcccg cgccgcccgg gctcctgctg gcgctcagcg
      841 ggagttggca cgggcacgag gccacgctgc gcagcgcgct gacacgctgc gccgccgagg
      901 ccgaccacct gcgcatcgac ctgacaggcg ccgccgaact cgacaacgcg gcgatcgcgc
      961 tgctgctgat cgcgcgcggc tggttcgagg cccgcggtgg cttcgaggtc gtcggcgcgt
     1021 cgcgcacact gagcgtcggc ctcaggcgca agctcgcgac cgcggccttg ctcggggccg
     1081 accgatgaac cgccaggacg cgccagaact gcgagctgaa ccgcggatct cggccgagac
     1141 gccggactgg tcgcgcgagg caaagccgtt ctggcactgg agcccctcgc gctcgctgct
     1201 ggcgagcctg cgtgcctacg agcgctcgcg gctctcggcg cggccggacc aggtgctgcg
     1261 gcgctggctc gcggtgctgc gccaccggtt ctggagcgcc gtaacaggcg cggagatccc
     1321 gctcgggacg cgcatcgacg gcggcctgat gatgacccac ccaaacggca tcgtcgtcca
     1381 ctgcgacgcg cggatcggcc ccaactgcct gattctccag caggtgacgc tgggtgtcgg
     1441 cggggcgcgg cccggcgcgc cgacgctcgt cgggcactgc gacatcggcg cgggcgccaa
     1501 gctgctcggc ggcatcacga tcggcgcgca cgcggtcgtc ggcgcgaacg ccgtggtgtt
     1561 gtgcgacgtt cccgccgacg caacggcggt cggcatccct gcgcgcgtgc tgtcggcgcg
     1621 ctcgacgaaa gcgctggatg gctgacagct tgcgggttct catcgcggcc gagcacgcct
     1681 cggcgcgctt cggcggcgag gcggcgctgc cgctgcacta cttccgcgtg atgcgcgcgc
     1741 gcggcctgcc ggtctggctg gtgacgcacg cgcgcacccg ggatgaactc tcggcgctgt
     1801 acccgaacga tccgaacatc gtctacatcg aggacacggc atggcaccgc gcgatgtggc
     1861 gcctggggca gcgcttgccc gacgccatct cgtacgtcac gaccggattc ctctcgcgcc
     1921 tgtctaccca gtgggcgcag cggcgcatcg tcagcgacat ggtgaagcgg cacggcatcg
     1981 atgtggtcca ccagccgatg ccggtgtcgc cgcgcgagcc gtcgatgctg tatggcttcg
     2041 gcgtgccggt cgtgatcggg ccgatgaacg gcgggatgga ctacccgccc gctttccgcc
     2101 gccagcgcgg cgttgccgag cgctggctgc tgggcgccgg gcgcgcgagc gccgccttgc
     2161 tgaattgcct gatacccggc aagcgccgcg cggcgaccct gctggtcgcc aacgagcgca
     2221 cgcgcgcggc gctgccggct ggcgtatgca cgaatgtggt cgggctcgtc gagaacggcg
     2281 tcgacctcgg cgtgtggcgc ccgacgccgc agcccgaggg cgcggccgcg caagacggcg
     2341 tcacgacctt cgtcttcatg ggccggctgg tcgactggaa ggcggtcgac ctgctcctgc
     2401 acgcattcgc gcgggcgcgc gaccaagcgc cgatgcggct ctggatactc ggcgacggca
     2461 gcgagcgcgc gcgcctcgaa gggctcgccg gcacgctcgg cgtcgcgacc cggccgtccg
     2521 ctggcgccgg cgcgatcgag tttgccggat ggctgccgca ggcgacctgc gccgcccgcc
     2581 tcgcc
//
LOCUS       DASRDG010000145         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_255618, whole genome shotgun sequence.
ACCESSION   DASRDG010000145 DASRDG010000000
VERSION     DASRDG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255618"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFA35_02360"
     CDS             complement(<1..951)
                     /locus_tag="VFA35_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZT55042.1"
                     /translation="MNGTGSASANGLLMKAVFRSGVPVVGKNYFPSNIQGLPTWYEIR
                     VTRDGHRARSGKVDIMVALNVETYARDLQEVSSGGYFLYDSTWPRPALTMRDDVTVLG
                     VPFARLCNENFSGVRNRILLKNICYAGALAALHGIDLDIIRTLLKETYAKKPKLVDSN
                     MQAIQLGYDYTQQNFSCPLPLRIEKLDATAGHVIIDGNTAAALGCVYAGATVGAWYPI
                     TPSTSLMDAFKSFSDRLRVDPVTGRKNFLMIQAEDELASIGMVIGAAWNGARAFTATS
                     GPGISLMQEFLGLAYYTETPAVVFDVQRVGPSTGMPTRTQQ"
     gene            complement(978..>2659)
                     /locus_tag="VFA35_02365"
     CDS             complement(978..>2659)
                     /locus_tag="VFA35_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT55043.1"
                     /translation="AYLLNRESNVFPGILGRVCDRPCEPACRRGRVEEKPVAICRLKR
                     VAADLRGDLTGRLPRAPFERNGKRIACVGAGPASLTVANDLAPLGYEVVIFERLATPG
                     GLMRSNIPAFRLPAAVLDDEIGMILDMGVELRLDAPVASMRELLERQGFDAVFVGSGA
                     PKGKELELPGRRDTDRIHIGIDWLESVAFEHIDKIGERVLIIGVGNTAMDCCRTSLRL
                     GARSVKVMARKPRGFFKASDWELEDAEAENVEIVVNRSPKRFVIEGGKLTGMVFELME
                     YELKDGRITAERVTGEELLPCDDVILAIGQENAFPWIERDLGIEFDKWSVPKVDATTF
                     QSTRPGVFFGGDAAFGPKNIIWAVEHGHQAAISLHQYLQGKSLAERPASSVKLGSVKM
                     GMHEWAYANDYATTERRLVPHVSLKERFKKVDIEVELGFKPEQAAQETHRCLNCDVQT
                     VFDAKLCIECDACIDVCPVDCLTITPNGEEAELRTRLRAPAKVATQPLYVSRALPQTG
                     RVMVKDENLCVHCGLCAERCPTAAWDMQKSTIHWPHAGDGEPACHTQRQIA"
BASE COUNT          416 a          866 c          909 g          468 t
ORIGIN      
        1 ctgctgcgtg cgggtcggca tgccggtcga ggggccgacg cgctgcacgt cgaacacgac
       61 cgccggagtc tcggtgtagt aggcgagccc gaggaattcc tgcatcagcg agatgccggg
      121 tccggaagtg gcggtaaacg cgcgcgcgcc attccaggcc gcgccgatca ccatgccgat
      181 cgaggccagc tcgtcctcgg cctgtatcat gaggaaattc ttgcggccgg taaccgggtc
      241 gacgcgcagc cggtcgctga acgacttgaa cgcgtccatg agcgaagtcg agggcgtgat
      301 cgggtaccag gcgccgaccg tggcgccggc gtagacgcag ccgagcgccg ccgccgtgtt
      361 gccgtcgatg atgacgtggc cggccgtggc gtcgagcttc tcgatgcgca gcggcagcgg
      421 gcagctgaag ttctgctgcg tgtagtcgta accgagctgg atcgcctgca tgttgctgtc
      481 gacgagcttc ggcttcttgg cgtaggtctc cttgagcagc gtgcggatga tgtcgaggtc
      541 gatgccatgc agcgccgcca gcgcgccggc gtagcagatg ttcttgagca ggattcggtt
      601 gcgcacgccg ctgaagttct cgttgcagag ccgcgcgaac ggcacgccga gcaccgtaac
      661 gtcgtcgcgc atcgtcagcg ccggtcgcgg ccaggtggaa tcgtagagga agtagccgcc
      721 ggagctgact tcctgcaggt cgcgcgcgta ggtttcgacg ttgagcgcca ccatgatgtc
      781 gaccttgccg ctgcgggcgc ggtggccgtc gcgggtgacg cggatctcat accaggtcgg
      841 cagcccctgg atgttcgagg ggaagtagtt cttgcccacg accggcacgc cgctgcggaa
      901 cacggccttc atcagcaggc cgttggcact ggccgagccg gtgccgttca cggtcgcgat
      961 cttgatgacg aagtcgttta cgcgatttgg cgctgcgtat gacatgcggg ttcaccgtct
     1021 ccggcgtggg gccaatgtat ggtcgacttc tgcatgtccc aggccgccgt cgggcagcgc
     1081 tcggcacaca ggccgcagtg cacgcacagg ttctcgtcct tgaccatgac gcggccggtc
     1141 tgcggaaggg cgcgcgatac gtacagcggc tgcgtcgcca ccttcgccgg cgcgcgcagc
     1201 cgcgtgcgca gctccgcttc ctcgccgttc ggcgtgatcg tcaggcagtc caccgggcaa
     1261 acgtcgatgc aggcgtcgca ctcgatgcag agcttcgcgt cgaacaccgt ctgcacgtcg
     1321 caattgaggc agcggtgggt ctcctgcgcg gcctgttccg gcttgaagcc gagttcgacc
     1381 tcgatgtcga ccttcttgaa gcgctccttg agcgaaacgt gcggcaccag ccgccgctcc
     1441 gtcgtggcgt aatcgttggc gtaggcccac tcgtgcatgc ccatcttgac gctgccgagc
     1501 ttgacgctcg atgccgggcg ctcggccagc gacttgccct gcaggtactg atgcaaggag
     1561 atggcggcct ggtggccgtg ctcgaccgcc cagatgatgt ttttcggccc gaaggcggcg
     1621 tcgccgccga agaacacgcc cgggcgcgtc gactggaacg tggtcgcatc caccttcggc
     1681 acgctccact tgtcgaactc gatgccgagg tcgcgctcga tccagggaaa ggcgttttcc
     1741 tggccgatcg caaggatcac gtcgtcgcag ggcagcagct cctcgccggt gacgcgttcg
     1801 gccgtgatgc ggccgtcctt gagttcgtat tccatgagct cgaagaccat gccggtcagc
     1861 ttgccgcctt cgatcacgaa gcgcttcggc gagcggttga cgacgatctc gacgttctcg
     1921 gcctcggcgt cctcgagctc ccagtccgag gccttgaaga atccgcgcgg cttgcgcgcc
     1981 atgaccttga cgctgcgcgc gccgaggcgc agcgaagtgc ggcaacaatc catggccgtg
     2041 ttgccgaccc cgatgatcag cacgcgttcg ccaatcttgt cgatgtgctc gaaagcgacc
     2101 gactcgagcc agtcgatgcc gatatggatg cgatcggtgt cacgccggcc gggcagctcg
     2161 agttccttgc ccttgggcgc ccccgagccg acgaagacgg cgtcgaaccc ctgtctttcc
     2221 agcagttcgc gcatgctcgc caccggcgca tcgagccgca gctcgacgcc catgtcgagg
     2281 atcatgccga tctcgtcatc gagcacggcg gcaggcaggc ggaaggccgg aatattgctg
     2341 cgcatgagcc cgcccggcgt agccaggcgc tcgaagatca cgacttcgta gccgagtggc
     2401 gccaggtcat tcgcgaccgt gagcgacgcg ggcccggcac cgacgcaggc gatgcgcttg
     2461 ccattgcgct cgaacggcgc gcgcggcagc cggcccgtca ggtcgccgcg caagtcggcg
     2521 gcgacgcgct tgaggcggca gatcgcaacc ggcttctcct cgacgcggcc gcggcggcag
     2581 gccggctcac acgggcggtc gcagacgcgg cccaggatgc ccgggaagac gttcgactcg
     2641 cggttgagca ggtaggcat
//
LOCUS       DASRDG010000146         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_25272, whole genome shotgun sequence.
ACCESSION   DASRDG010000146 DASRDG010000000
VERSION     DASRDG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25272"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFA35_02370"
     CDS             <1..240
                     /locus_tag="VFA35_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZT55044.1"
                     /translation="LRLEQPPQQHMRIEQQGFRLLPLRVEHVMAAADIGSAHGDPTDR
                     MLLGTAYAERMAFATRDASLLELAAPLMGTLLVEA"
     gene            complement(245..2020)
                     /gene="lpdA"
                     /locus_tag="VFA35_02375"
     CDS             complement(245..2020)
                     /gene="lpdA"
                     /locus_tag="VFA35_02375"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZT55045.1"
                     /translation="MALIEIKVPDIGDFKDVAVIEVLVKPGDTVKVEQSLLTVESDKA
                     SMEIPSSHAGVVKSVLVKVGDTVNEGSVIVSLDAEGAAAVAAPAPAVPSAAPTSAPAA
                     AAAPAPVAATYAGGADVECDLVVIGAGPGGYSAAFRAADLGLKVVLVERFATLGGVCL
                     NVGCIPSKALLHVAAVMDEVKHFDSYGITFQPPVVDLAKLKTHKEKVVGKLTGGLAAM
                     AKMRKVTVVQGTAEFLDPFHIAVDLAKGGKQTIKFKQAIIAAGSEAVKLPFLPKDDPR
                     VVTSTGALELRQNPKRMLIVGGGIIGLEMGTVYSTLGARLDVVEMLDGLMQGADRDLV
                     KVWQKMNVPRFDNIMLKTKTVGAEVRPDGILVKFENADGSPAAVKEQVYDLVLQAVGR
                     VPNGKRIGADKAGVAVTDRGFIPVDIQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVA
                     AEVAAGDTKAAFDARVIPSVAYTDPEVAWVGVTEDEAKAKGMKVKKGLFPWNASGRAI
                     ANGRDEGFTKLIFSEETHRIVGGGIVGTHAGDMIGELALAIEMGADAFDIGKTIHPHP
                     TLGESIGLAAEAFEGACTDLPPVKK"
     gene            complement(2023..2604)
                     /locus_tag="VFA35_02380"
     CDS             complement(2023..2604)
                     /locus_tag="VFA35_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT55046.1"
                     /translation="MTTVVVARKGNTVAIAADTLVTFGDTRLGHGYEANLKMFKAGDS
                     WIGMAGTTAHFPVVRRALASLPPDELRLHSRDQVFETFLKLHPKLKETFFLNTKEEDS
                     DPYESSQLTALIANHAGIFGVYSYREVFEFDRFWGIGTGRAFALGAMFASYDKAKTAR
                     EIAELGVRAGCEFDKNSSGPFHVHTIKLKASKG"
BASE COUNT          386 a          907 c          864 g          459 t
ORIGIN      
        1 ctgcggcttg agcagccacc acagcagcac atgcgtatcg agcagcaagg ttttcggctg
       61 ctgccgctgc gcgtcgagca tgtgatggcg gcggccgaca tcggcagcgc gcacggcgac
      121 cccacggacc gaatgctgct gggcaccgcc tacgccgagc gcatggcctt cgccacgcgc
      181 gacgcgagcc tgctggaact ggccgcgccg ttgatgggca ccctgctggt ggaagcctga
      241 cgcgctactt cttcaccggc ggcaggtcgg tgcacgcccc ctcgaaggcc tccgccgcca
      301 ggccgatgct ctcgccgagc gtcgggtgcg ggtggatcgt cttgccgatg tcgaacgcgt
      361 cggcgcccat ctcgatcgcg agcgcgagct cgccgatcat gtcgccggca tgggtgccga
      421 cgatgccgcc gccgacgatt ctgtgcgtct cctcgctgaa gatcagcttc gtgaagccct
      481 cgtcgcggcc gttggcgatc gcgcggccgg aggcgttcca cgggaacagg cccttcttca
      541 ccttcatgcc cttggccttg gcttcgtcct cggtgacgcc gacccacgcg acctccgggt
      601 cggtgtaggc cacgctcggg atcacgcgtg cgtcgaacgc cgctttggtg tcgccggccg
      661 ccacctcggc cgccacgtgc gcctcgtgca ccgccttgtg cgccagcatc ggctggccga
      721 cgatgtcgcc gatcgcgaag atgtgcggca cgttggtgcg catctggatg tccaccggga
      781 tgaagccgcg gtcggtcacc gccacgccgg ccttgtcggc gccgatgcgc ttgccgttcg
      841 gcacccggcc gacggcctgc agcaccaggt cgtagacctg ttccttgacc gccgccggcg
      901 agccgtcggc attctcgaac ttgacgagga tgccgtccgg ccgaacctcg gcgccgacgg
      961 tcttggtctt cagcatgatg ttgtcgaagc gcggcacgtt catcttctgc cagaccttca
     1021 cgaggtcgcg gtcggcgccc tgcatcaggc cgtcgagcat ctcgaccacg tccaggcgcg
     1081 cgccgagcgt cgaatagacc gtgcccatct ccaggccgat gatgccgccg ccgacgatca
     1141 gcatgcgctt cgggttctgg cgcagctcga gcgcgccggt cgaggtgacg acgcgcggat
     1201 cgtccttcgg caggaacggc agcttcaccg cttcggagcc ggcggcgatg atggcctgct
     1261 tgaacttgat ggtttgcttg cctcccttcg ccaggtcgac ggcgatgtgg aacggatcga
     1321 ggaactcggc cgtgccttgc accacggtga ccttgcgcat cttcgccatc gcggccaggc
     1381 cgccggtcag cttgccgacg accttctcct tgtgcgtctt gagcttcgcg aggtcgacga
     1441 ccggcggttg gaacgtgatg ccgtagctgt cgaagtgctt gacctcgtcc atcaccgcgg
     1501 cgacgtgcag cagcgccttc gacgggatgc agccgacgtt caggcacacg ccgccgagcg
     1561 tcgcgaagcg ctcgaccagc acgaccttca gccccaggtc ggcggcgcgg aacgcggccg
     1621 agtagccacc cgggccggcg ccgatcacga ccagatcgca ctcgacatcg gcgccgccgg
     1681 cgtaggtggc ggcgaccggc gcgggcgctg ccgcagcggc cggcgcagac gttggcgcag
     1741 ccgaaggcac ggcaggcgcc ggggccgcga cggctgcagc gccttccgcg tcgagcgaca
     1801 cgatcaccga gccttcgttg acggtgtcgc caaccttgac cagcacgctc ttcacgacgc
     1861 cggcgtgcga cgacgggatc tccatcgagg ccttgtcgct ctcgacggtc agcagcgact
     1921 gttcgacctt cacggtgtcg ccgggcttca cgagcacctc gatcaccgcc acgtccttga
     1981 agtcgccgat gtccggcacc ttgatctcga tgagggccat ggtcagcctt tcgatgcttt
     2041 gagtttgatg gtgtggacgt ggaacggccc actggagttc ttgtcgaatt cgcagcccgc
     2101 gcgcacgccg agttcggcaa tctcgcgcgc ggtcttcgcc ttgtcgtagc tcgcgaacat
     2161 cgcgccgagc gcgaacgcgc ggccggtgcc gatgccccag aagcggtcga actcgaacac
     2221 ctcgcggtac gagtacacgc cgaagatgcc ggcgtggttc gcgatcagcg cggtgagctg
     2281 cgaactctca tacggatcgg agtcttcttc cttcgtgttc aggaagaaag tctccttcag
     2341 cttcggatgc agcttcagga aggtctcgaa cacctggtcg cgcgagtgca ggcgcagctc
     2401 gtcgggcggc aggctcgcca gcgcgcgccg cacgaccggg aagtgcgcgg tcgtgccggc
     2461 catgccgatc cacgaatcgc cggccttgaa catcttcagg ttcgcttcgt agccatggcc
     2521 gaggcgcgtg tcaccgaagg tgaccagcgt gtcggcggcg atggccacgg tgttgccctt
     2581 gcgggccaca accaccgtcg tcatcgaacc tcctgc
//
LOCUS       DASRDG010000147         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_25759, whole genome shotgun sequence.
ACCESSION   DASRDG010000147 DASRDG010000000
VERSION     DASRDG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25759"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..379
                     /locus_tag="VFA35_02385"
     CDS             230..379
                     /locus_tag="VFA35_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HZT55047.1"
                     /translation="MPKVHSHYENLKVARDASIDDIRGAYRALTRRHHPDRNPHNADP
                     SGSWL"
     gene            370..1179
                     /locus_tag="VFA35_02390"
     CDS             370..1179
                     /locus_tag="VFA35_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55048.1"
                     /translation="MAVINVAYAVLCDPVKRAEHDRWIAQAEATPVPLRAARPVRGKP
                     TLHAPTDRYLEPRPAEPESAAARRARQLRLDRRVRRLAAHLLRHRVRYAVAGIAALGL
                     IGAGLAPLFEPAMVAPTAGTPAQAAPAVPGYVRAAAAPNGQPWPNRSGYVAGYLQTNH
                     GGLSEITVDNGHNDADMFAKLFSLDGPSAQPVRTFFVAAHSRFTLTGLTIGNYDLRYR
                     NLASGGLLRSPAFILEEVKTEAGTQRSTPTMKLHQSSDGSLQTYALGDAEF"
     gene            complement(1190..>2288)
                     /locus_tag="VFA35_02395"
     CDS             complement(1190..>2288)
                     /locus_tag="VFA35_02395"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZT55049.1"
                     /translation="DVIKIEAPDGGDPLRGWRLVKDGTSVWWQVSSRNKRSIALDLRA
                     AEGQQIARQLIQEADVLVENFRPGTLEGWGMGWDDLRALNPGLVMLRISGYGQTGPYR
                     DLPGFGAIGEAMGGLRHLTGEPGRVPVRVGVSIGDTLAALHGTIGVLTALYHREVNRG
                     AGQVIDVALHEAVFNVMESLIPEYSAFGAVREAAGSALPGIAPSNAYHCADGYVLVAG
                     NGDSIFKRLMAAIGRADLAAAPDLANNAGRVARVAEIDAAIERWTEARRVEEVLAVLG
                     AARVPAGKVYTAKDIHEDPHFRAREMILTQTTRDGYTLDVPGVVPKLSATPGTLRSAA
                     PHLGDDTDSVLAEIGLSPAQIAQLRERGIVA"
BASE COUNT          328 a          834 c          801 g          325 t
ORIGIN      
        1 cagttcacca ccgaagtgaa gccgccggcg tcgaacagca ggtcgccgtc ggtcatcacg
       61 cggtactcgt acacctcgtc ggtgcggtgg acttcgatgc gcacgatcat gtccagattc
      121 tgatcgaaag cggcgcgtgc gtgcatgagt gcgtgcgtgg ggccgcctgc gctgcggcat
      181 gatcgcgaac cggcgcgggc cgagtctttt cgtcttccgc gcgaccccga tgcccaaggt
      241 ccacagccac tacgagaacc tcaaggtcgc gcgcgatgcg tcgatcgacg acatccgcgg
      301 cgcgtaccgg gcgttgacgc gccggcacca ccccgaccgc aacccgcaca acgccgatcc
      361 gagcgggtca tggctgtgat caacgtggcc tacgcggtgc tttgcgaccc ggtcaagcgc
      421 gcagagcacg accgctggat cgcgcaggcc gaagcgacac cggtgccgct gcgcgccgcc
      481 aggccggtgc gcggcaagcc gaccctgcac gcgccgaccg atcgctacct cgagccgcgc
      541 cccgccgagc ccgaatccgc cgccgcgcgg cgcgccaggc agttgcggct ggaccggcgt
      601 gtgcgccgcc tcgccgcgca cctgctgcgc catcgggtgc gctatgcggt cgcaggcatc
      661 gctgcgctgg gcctgatcgg ggctgggctg gcgccgctgt tcgagccggc gatggtcgcg
      721 ccgacggccg gcacgccggc ccaggccgcg cctgccgtgc ccggctacgt gcgcgcggcg
      781 gcggcgccga acggtcagcc gtggccgaac cggtccggct acgtggcggg ctacctgcag
      841 accaaccacg gcgggctgtc ggagatcacg gtcgacaacg ggcacaacga cgccgacatg
      901 ttcgccaagc tgttctcgct ggacggcccg agcgcgcagc cggtgcgcac cttcttcgtc
      961 gccgcacaca gccgcttcac gctcaccggg ctgacgatcg gcaactacga cctgcgctac
     1021 cgcaacctcg ccagcggcgg cctgctgcgc tcgccggcct tcatcctcga agaagtgaag
     1081 accgaggccg gtacccagcg cagcacgccg acgatgaaac tgcaccagag cagcgacggc
     1141 agcctgcaga cctacgcgct cggcgacgcc gagttctgag caagcgcgct caggcgacga
     1201 tgccgcgctc gcgcagttgc gcgatctgcg cgggcgacag gccgatctcg gcgagcacgc
     1261 tgtcggtgtc gtcgccgaga tgcggcgcgg cgctgcgcag cgtgcccggc gtggccgaga
     1321 gcttgggcac gacgccgggc acgtcgagcg tgtagccgtc gcgcgtggtc tgggtcagga
     1381 tcatctcgcg ggcgcggaag tgcgggtctt cgtggatgtc cttcgcggtg tagaccttgc
     1441 cggccggcac acgcgcggcg ccgagcacgg ccagcacctc ctcgacgcgg cgcgcttcgg
     1501 tccagcgctc gatcgcggcg tcgatctcgg ccacgcgcgc gacacggccg gcgttgttgg
     1561 cgaggtcggg tgctgcggcc agatcggcgc ggccgatcgc cgccatcagg cgcttgaaga
     1621 tgctgtcgcc gttgccggcg acgagcacgt agccgtcggc gcagtggtag gcgttcgatg
     1681 gtgcgatgcc gggcagcgcg ctgccggcgg cctcgcgcac cgcgccgaat gcgctgtact
     1741 cggggatcag gctctccatc acgttgaaca ccgcctcgtg cagcgccacg tcgatcacct
     1801 ggcctgctcc gcgattgacc tcgcggtggt acagcgcggt cagcacgccg atcgtgccgt
     1861 gcagcgcggc gagcgtgtcg ccgatcgaca cacccacgcg caccggcacg cggccgggct
     1921 cgccggtcaa atggcgcagc ccgcccatcg cctcgccgat cgcgccgaag ccgggcaggt
     1981 cgcggtacgg cccggtctgg ccgtagcccg agatgcgcag catcaccagg cccgggttca
     2041 gcgcacgcag atcgtcccag cccatgcccc agccttcgag cgtgccgggg cggaagttct
     2101 cgaccagcac gtcggcctct tggatcagct ggcgggcgat ttgctggccc tcggcggcgc
     2161 gcaggtcgag cgcgatcgag cgcttgttgc gcgaagacac ctgccaccag accgaggtgc
     2221 cgtctttcac gagccgccag ccgcgcagcg ggtcgccgcc gtccggcgct tcgatcttga
     2281 tcacgtcg
//
LOCUS       DASRDG010000148         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_726405, whole genome shotgun sequence.
ACCESSION   DASRDG010000148 DASRDG010000000
VERSION     DASRDG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726405"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFA35_02400"
     CDS             complement(<1..284)
                     /locus_tag="VFA35_02400"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192504.1"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZT55050.1"
                     /translation="MSDTLKRDALDYHRYPTPGKISVTPTKAMLTQRDLALAYSPGVA
                     EACMAIVDDPLEAGNLTSRANLVAVVTNGTAVLGLGNIGPLASKPVMEGKG"
     gene            complement(299..>2646)
                     /locus_tag="VFA35_02405"
     CDS             complement(299..>2646)
                     /locus_tag="VFA35_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZT55051.1"
                     /translation="FMVTEKMSVHDAREIRAMGQSNGIDIFGANCLGVADAWNQVRIG
                     GALGGDAPGEALKRGSIAIFSNSGNFTTTIATYLRMAGWGTTTLISSGKDVYIHYAAP
                     EFAFALANDDRSKAAVLYCEPGGYYERDANFTKPVVACVVGRWKSKLTRAVGHAGAMA
                     GGADDAASKERWFMEKFGVDSLFTPQNPVVSAKGAVVVNIADIPAALTAVMRANGVQP
                     DFAPEGTMELKPWFISTRGLDLPAELDLPTVEAPAPYNEQIEALKRQIGAVLPRQTMK
                     DTSGASQMDAKTQLTSLYGVSMLDAAQYQLEGNVSLALLHEPGGANDQRLINVAIGSA
                     LNLHGHPALAAAQAAREAGNAPNTVLAMAAGIFGPKSQDGARRAVQLLIEHFAAAGLA
                     DAQDESFDLARVKIGNTEPLVGKKPDARARAMLAGLKARGAKSVFVRYLESLPGHPTA
                     DAVLAAITTTLAWGPLMRKRVSRLTAESLPWWMRLFGALIGASVPAAQHTADAFCGIS
                     TSDILERRPISEVAFIALLGLPPTPADVFSFQLLVGLLLTNGPGTISAQGAKGAVSAD
                     GPEEPARVQLNKGLVGFLTHTGYAHGGNGYEGIAFLIEQFKDAGLEDPGDPNHGIDLR
                     ALAMKYVHAYAGYKSSKKNTGSLDIQKIPGVNHPVFKDRPVNHDPREVFIAKLFAQRG
                     EYNVFHEFYRVLVQALFDGGVSRTVYCVNIDAVIAAVLLKMLWKPYRSGQYSAATLET
                     AAFTIFLYPRMLGCAAEIDDHLNRGRNMDTRTPASQCKFVA"
BASE COUNT          373 a          913 c          915 g          445 t
ORIGIN      
        1 cccttgccct ccatcaccgg cttgctcgcc agcgggccga tgttgcccag gccgagcacc
       61 gcggtgccgt tggtgacgac cgcgacgagg ttcgcgcgcg acgtgaggtt gcccgcctcc
      121 agcgggtcgt cgacgatggc catgcaggct tcggccacgc cgggcgagta ggccagcgcg
      181 agatcgcgct gcgtcagcat cgccttggtc ggcgtcaccg agatcttgcc gggggtcgga
      241 tagcggtggt aatccagcgc gtcgcgcttg agggtgtcgc tcatttgtct gtgtctccct
      301 aggccacgaa cttgcactgc gatgcaggcg tgcgtgtgtc catgttgcgg ccgcgattga
      361 ggtggtcgtc gatctcggcc gcgcagccga gcatgcgcgg gtacaggaag atcgtgaacg
      421 cggcggtctc cagcgtcgcg gcgctgtact ggccgctgcg gtacggcttc cagagcatct
      481 tcagcagcac cgcggcgatc accgcgtcga tgttgacgca gtacacggtg cgcgagaccc
      541 cgccgtcgaa cagcgcctgc accagcacgc gatagaactc gtggaacacg ttgtactcgc
      601 cgcgctgcgc gaacagcttg gcgatgaaca cttcgcgcgg gtcgtggttg accggccggt
      661 ccttgaacac cgggtggttc acgccgggga tcttctggat gtccaggctg ccggtgttct
      721 tcttgctcga cttgtagccg gcgtaggcgt ggacgtactt catcgccagc gcccgcaggt
      781 cgatgccgtg gttcgggtca ccggggtcct cgagccccgc atccttgaac tgctcgatca
      841 ggaaggcgat gccctcgtag ccgttgccgc cgtgcgcgta gccggtgtgc gtcaggaagc
      901 cgaccaggcc cttgttcagc tgcacgcgcg ccggctcttc cggcccgtcg gccgagaccg
      961 cgcccttcgc gccctgcgcc gagatcgtgc cgggcccgtt ggtcagtagc agcccgacca
     1021 agagctggaa cgagaacacg tcggccggtg tcggcggcaa gccgagcagc gcgatgaacg
     1081 cgacctcgct gatcgggcgg cgctcgagga tgtcgctcgt cgagatgccg cagaaggcat
     1141 cggccgtgtg ctgcgcggcg ggcaccgagg cgccgatcag cgcgccgaac aggcgcatcc
     1201 accacggcag gctctcggcc gtcagacgcg acacgcgctt gcgcatcaac ggcccccagg
     1261 ccagcgtggt ggtgatcgcg gccagcacgg cgtcggccgt cgggtggccc ggcaggctct
     1321 ccaagtagcg cacgaacacc gacttcgcgc cgcgcgcctt caggccggcc agcatcgcgc
     1381 gggcccgcgc gtcgggcttc ttgcccacca gcggttcggt gttgccgatc ttgacgcggg
     1441 cgaggtcgaa gctctcgtcc tgcgcgtccg ccagaccggc cgcggcgaag tgctcgatca
     1501 gcagctgcac ggcgcggcgc gcgccgtcct gcgacttcgg cccgaagatg ccggccgcca
     1561 tcgcgagcac cgtgttcggc gcgttgccgg cctcgcgcgc cgcctgggcc gcggccagcg
     1621 ccggatgacc gtgcaggttc agcgccgagc cgatcgcgac gttgatcagg cgctgatcgt
     1681 tggcgccgcc gggctcgtgc agcagcgcca ggctgacatt gccctcgagc tggtactgcg
     1741 cggcatcgag catcgagacg ccgtacaggc tggtcagctg cgtcttcgca tccatctgcg
     1801 acgcgcccga ggtgtccttc atggtctggc gcggcagcac cgcgccgatc tggcgcttca
     1861 gcgcctcgat ctgttcgttg tacggcgccg gtgcctcgac cgtcgggaga tcgagctccg
     1921 ccggcagatc caggccgcgc gtggagatga accagggctt cagctccatc gtgccctcgg
     1981 gcgcgaagtc gggctgcacg ccgttggccc gcatcaccgc ggtcagcgcc gcgggaatgt
     2041 cggcgatgtt gacgacgacc gcgcccttgg ccgagaccac cgggttctgc ggcgtgaaca
     2101 gcgaatcgac gccgaacttc tccatgaacc agcgttcctt cgacgccgcg tcgtcggcgc
     2161 cgccggccat cgcgccggcg tggccgacgg cccgcgtcag cttgctcttc cagcgcccca
     2221 ccacgcaggc gaccaccggc ttggtgaagt tcgcgtcgcg ctcgtagtag ccgcccggct
     2281 cgcagtacag caccgcggcc ttgctgcggt cgtcgttggc gagcgcgaac gcgaactcgg
     2341 gcgccgcgta gtggatgtag acgtccttcc cgctggaaat gagcgtcgtg gtgccccagc
     2401 cggccatgcg cagatacgtg gcgatggtgg tcgtgaagtt gccggagttg gagaagatcg
     2461 cgatcgagcc cctcttcaac gcctcgccgg gcgcgtcgcc gccgagcgcg cctccgatgc
     2521 gcacctggtt ccacgcatcc gccacgccga ggcagttggc gccgaagatg tcgatgccgt
     2581 tcgactggcc catcgcgcga atctcgcgcg cgtcgtggac ggacattttc tcggtgacca
     2641 tgaaga
//
LOCUS       DASRDG010000149         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_137971, whole genome shotgun sequence.
ACCESSION   DASRDG010000149 DASRDG010000000
VERSION     DASRDG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137971"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..544)
                     /locus_tag="VFA35_02410"
     CDS             complement(71..544)
                     /locus_tag="VFA35_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HZT55052.1"
                     /translation="MTPKLILSAAALALAGAIAHAHSFKLGEIEIGHPYARATAAGQP
                     TGGGFLKLDNKGRDDKLLSVRAAVSAGVELHAMRMDGDVMRMRQVDAIALPAGQTVEL
                     KPGGFHIMFVGLKAPLKAGDKFPMTLTFEKAGEVEVTVNVEAPKAAADAPAEHKH"
     gene            complement(559..2859)
                     /locus_tag="VFA35_02415"
     CDS             complement(559..2859)
                     /locus_tag="VFA35_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZT55053.1"
                     /translation="MLHAQTVADDNVPVKSLGVVTVTGGQPSSLPTQIPATMESVSAA
                     QIARTVNASDSEDALKYLPSLLVRKRYIGDYNHAILSSRASGTGNSARSAVYADGILL
                     SNYLGNGVGGLSFPPRWGMVAPEEIERVDVMYGPFSAAYPGNSVGAVVVFTTRMPSAF
                     EAHAKAGYDSQPFELYGTRATYRAWETSASVGNKAGAFTWLLNFNHADSHGQPLTFAT
                     RLLSGGTTGSAGMAVVGAVRDANNANQPWYLLGTGTEYQSKQDHFKARFAYDITPTIR
                     ANYVLGVWQNTSDSTPVSYLRNAAGQPVYSGVVNIGGQSFAPLTGADFAATTERLTHV
                     MHGLTVKSHTQGAWDWELAASRYDYRRDDKRQSAAGNNLPNALTGGAGTLADGSGTGW
                     NNLAAKGTWRPGGLSGLPGEHIVDFGLQQDSYRLHYLTSDLAGNWLADGTGALVSSVT
                     GKTQLRSAYAQDAWRFAERWKAVLGLRAEDWRAHDGATTFSATSTLRYPLRHERYLSP
                     KAALSYQVASDLVLKASAGRAVRMPTVSELYGATSTTNSQYINDPNLKPERSWTTELT
                     AEKDLGNALLRLTFFTESTRDALYSQTSFDPAANRNIGRVQNVGRIRTNGLELAFNGT
                     DVLADVLGKGLDLSGSVTYADSVIRANDGFVVAPGDTLGKRQPNVPRWRATALASYRF
                     DTRWSAAFGVRYSGTQYRTLNNADVNGASYQGASPYFTTDLRLRWQITRQWAAAFGID
                     NLNNDRYWNFHPYPQRSVNAELRFDL"
BASE COUNT          442 a         1025 c         1163 g          538 t
ORIGIN      
        1 gggcgtcagc gacggcatca agctggcgct gcgctcgctc gcgaagtaac gcccacccgc
       61 cttcggatcc tcaatgcttg tgctcggccg gcgcgtcggc ggccgctttc ggtgcctcga
      121 cgttcacggt gacctcgacc tcgccggcct tctcgaaggt caacgtcatc gggaacttgt
      181 cgccggcctt cagcggcgcc ttcaggccga cgaacatgat gtggaagcca cccggcttga
      241 gctcgacggt ctggccggca ggcagcgcga tggcgtcgac ctggcgcatg cgcatcacgt
      301 cgccgtccat gcgcatcgcg tgcaattcga cgccggccga aaccgccgcg cgcaccgaca
      361 gcagcttgtc gtcgcggccc ttgttgtcga gcttcagaaa gccgccgccg gtgggctggc
      421 cggccgcggt ggcgcgcgcg taggggtggc cgatctcgat ctcgccgagc ttgaacgaat
      481 gcgcgtgggc gatggcgccc gccagggcca acgcggcggc ggacaggatc agtttgggtg
      541 tcatggtgcg agtctccttc aaaggtcgaa gcgcagttcg gcgttgacgc tgcgctgcgg
      601 gtaggggtgg aagttccagt accggtcgtt gttcaggttg tcgatgccga acgcggccgc
      661 ccactggcgc gtgatctgcc agcgcaggcg caagtcggtc gtgaagtatg ggctcgcgcc
      721 ctggtagctg gcgccgttca cgtcggcgtt gttcagcgtg cggtactgcg tgccgctgta
      781 gcggacgccg aaggccgccg accaccgcgt gtcgaagcgg tagctggcga gcgcggtcgc
      841 gcgccatcgc ggcacgttcg gctggcgctt gccgagcgtg tcgccgggcg cgacgacgaa
      901 gccgtcgttg gcgcggatga cggagtccgc atacgtcacg ctgccgctca ggtccaggcc
      961 cttgcccagc acgtcggcga gcacgtccgt gccgttgaac gccagctcca gcccgttcgt
     1021 gcggatgcgg ccgacgttct ggacacggcc gatgttccgg ttcgcggccg ggtcgaagct
     1081 cgtctgcgaa tacagcgcgt cgcgcgtgct ttcggtgaag aaggtcaagc gcagcaacgc
     1141 gttgccgagg tctttctcgg ccgtcagttc ggtcgtccat gagcgctcgg gtttcaggtt
     1201 cgggtcgttg atgtactgcg agttggtcgt cgaggtcgcg ccgtagagct cgctgaccgt
     1261 gggcatgcgc accgcacgac cggccgaggc cttcagcaca aggtcggaag cgacctggta
     1321 cgacagcgcg gccttcggcg acagatagcg ttcatggcgc aacgggtagc gcagcgtgct
     1381 cgtggccgag aacgtggtcg caccgtcgtg cgcgcgccag tcttcggcgc gcaggccgag
     1441 caccgccttc cagcgctcgg cgaaacgcca cgcgtcctgc gcgtaagcgc tgcgcaactg
     1501 cgtcttgccg gtcacgctgc tgaccagcgc gccggtgcca tcggcgagcc agttgccggc
     1561 gaggtccgac gtgaggtagt gcagtcggta gctgtcttgc tgcaggccga agtcgacgat
     1621 gtgttcgccg ggcaagccgc tcaggccgcc ggggcgccac gtgcctttcg ccgcgaggtt
     1681 gttccagccg gtgccgctgc cgtcggccag cgtgccggcg ccaccggtca gcgcgttggg
     1741 caggttgttg cccgcggcgc tctggcgctt gtcgtcacgg cggtagtcgt accggctggc
     1801 ggcgagctcc cagtcccacg cgccctgcgt gtggctcttg accgtgaggc cgtgcatcac
     1861 atgggtcagg cgctctgtcg tcgccgcgaa atcggcgccg gtcaggggcg cgaacgactg
     1921 gccgccgatg ttgaccacac cgctgtagac cggctggccg gcggcgttgc gcaggtacga
     1981 gaccggcgtg ctgtcggacg tgttctgcca cacgccgagc acgtagttcg cacggatcgt
     2041 cggggtgatg tcgtaggcga acctggcctt gaagtggtcc tgcttcgatt ggtactcggt
     2101 gccggtgccc agcaggtacc agggctggtt tgcgttgttg gcgtcgcgca cggcgccaac
     2161 gactgccatg cccgcgctgc cggtcgtgcc gccgctgagc agccgcgtcg cgaaggtcag
     2221 cggctggcca tggctgtcag cgtggttgaa gttcagcagc cacgtgaacg cacccgcctt
     2281 gttgccgacc gacgcactgg tctcccaggc gcggtaggtg gcgcgggtgc cgtacagctc
     2341 gaagggctgg ctgtcgtagc cggccttcgc atgcgcctcg aaagcgctcg gcatgcgggt
     2401 cgtgaacacc acgaccgcgc cgaccgagtt gcccgggtag gcggccgaga acgggccgta
     2461 catcacgtcg acgcgctcga tctcctccgg cgcgaccatg ccccagcgcg gcggaaagct
     2521 caaaccgccg acgccgttgc caaggtagtt cgacagcaga atgccgtcgg cgtacacggc
     2581 cgagcgcgcg ctgttgccgg tgcccgaggc gcggctggag aggatcgcat ggttgtagtc
     2641 gccgatgtag cgcttgcgca ccagcaggct gggcaggtat ttcagcgcgt cttcgctgtc
     2701 gctcgcgttg acggtgcgcg cgatctgcgc ggcgctgacg ctctccatcg tcgccgggat
     2761 ctgcgtcggc agcgagctcg gctggccgcc ggtgacggtg acgacgccga gcgatttgac
     2821 ggggacgttg tcatcggcaa ccgtctgcgc gtgcagcatg agcggggcgg gcaaggccgc
     2881 gatgcaggcg agcgcgcggg gcaagaagtg acacatggtg ttgtcgatcc tgaacgggcg
     2941 gcggcagagc cgcagaccat gacacggggc cggcgtcgag ccccggtcaa gcgggcatcg
     3001 acgtctcggc agcggtgggt tcaggcgacg gccggcggcc cgtgcttgag ccgcgcgacc
     3061 caggtcgcac aggcatcggg cgcttgcgat gacgggtggc tccgcggcgg cgacgcatcg
     3121 cgcagcagca gcggcgcagg cgtgggcatg ggcgggtcga tgggcacc
//
LOCUS       DASRDG010000150         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_480181, whole genome shotgun sequence.
ACCESSION   DASRDG010000150 DASRDG010000000
VERSION     DASRDG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480181"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..>2521)
                     /locus_tag="VFA35_02420"
     CDS             complement(106..>2521)
                     /locus_tag="VFA35_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT55054.1"
                     /translation="VTLRENSPADVALGAKPIEVEGVAKAAAARVMSLARGGQTLLTR
                     EALAALGDTPLRVQSHGHWRMKGFAEPIELFEVGTADTVFAPPEDSAKVYRVARKDSL
                     WVPVRQIDNNLPEQVASFVGRARELDELLQRLSQARLLTLYGSGGVGKTRLLLQLAGA
                     VLGEFPDGAWVAELAPLVDPKLVPQTVATVLGLKEVPGKPITQTLAEHLKDRQALLLL
                     DNCEHLLDACAPLAEALLRACPQVKMLASSREALGIGGEQVFRVPSLALPEPDRKATS
                     ESVAQSEAVQLFVDRAALVRPDFRLTDRNAPSIASICRRLDGIPLAIELAAARMRSLS
                     AEEINRRLDQRFLLLTGGSRTALPRQQTLRSLIDWSYDLLQEPERLALQRLSVFAEGW
                     TEAAAAQVCAGDGVQLQDVPDLLASLCDKSLVLAEERDDHTRYRLLETVRQYARERLL
                     EQMSGEAVRGRHLECYLQHVEQAAAGLQGAEQMRWLRDLEEEHDNLRAALEWSLVAPT
                     PAAATNGLRLCAALQRFWLMRGHLSEGRACCARILQMTAAAAPTRERADALNAAGVLA
                     YHQHDHAAAREQHTQSLAIRRALGDRRGAAISLNNLGMLAADRGDLAAARVLHEESLA
                     IARELGNRNGIARSLGNLGMVALDQEDLVSARILFEDSLAMLRQLNDQDGIAISLHLL
                     GDVARKAGDLESARACFAESLMILRALGHKVRIVYSLDELAAVAGALGSPPAAARLWG
                     AAERLREEMGSPAGPGEPGADARVAAARAALQDDAAFELAWREGRGWSLDQAIAYALA
                     PTAAAG"
BASE COUNT          366 a          930 c          866 g          359 t
ORIGIN      
        1 ccgaagcgac ctgggtggtc acgcgggccg ccgagatctg cgaatggggg ccgccggacc
       61 tttgagaatg ggtcgaacag aggcggcggc cgtccgccta ccgggtcagc ctgccgcggc
      121 cgtaggcgcc aacgcgtacg cgatcgcctg gtcgagcgac cagccccggc cctcgcgcca
      181 ggcgagttcg aaggccgcgt cgtcctgcag cgccgcacgt gctgcggcaa cgcgggcatc
      241 ggcgcccggc tcgcctggcc cggcgggcga gcccatctct tcgcgcagcc gttccgccgc
      301 gccccagagt cgggccgcgg cgggcgggct gccgagcgcg ccggcgaccg ccgccagctc
      361 gtccagcgag tagacgatgc gcaccttgtg gcccagtgcg cgcaggatca tcaggctctc
      421 cgcgaagcag gcgcgggccg attcgaggtc gcctgccttg cgggccacgt cgcccaggag
      481 gtgcaagctg atcgcgatgc cgtcctggtc gttcagctgg cgcagcatgg cgagcgagtc
      541 ctcgaacaga atccgcgccg acacgagatc ctcctggtcc agcgcgacca tgcccaggtt
      601 ccccagcgag cgcgcgatgc cgttgcggtt gcccagctcg cgagcgatcg ccaggctctc
      661 ctcgtgcagc acgcgggccg ccgcgagatc gccccggtcg gccgccagca tgcccaggtt
      721 gttgagcgag atcgcggctc cccgccggtc gcccagcgcg cggcggatgg ccaggctctg
      781 cgtatgctgc tcgcgcgcgg cagcgtggtc gtgctggtga taggcgagca cgcccgccgc
      841 gttgagcgcg tccgcacgct cgcgcgtcgg cgccgcggcg gcggtcatct gcaggatgcg
      901 tgcacagcac gcacgccctt ccgacaaatg accgcgcatc agccagaacc gttgcagcgc
      961 cgcgcacagc cggaggccat ttgtcgctgc cgccggcgtc ggtgcaacca ggctccactc
     1021 cagcgccgcc cgcaggttgt cgtgctcctc ctcgaggtcg cgcagccagc gcatctgctc
     1081 ggccccctgc agcccggccg ctgcctgctc gacgtgctgc aggtagcact ccagatgccg
     1141 gccgcgaacc gcctcgccgc tcatctgttc cagcaagcgc tcgcgggcgt actggcgcac
     1201 cgtctcgagc aaccggtagc gcgtgtgatc gtcgcgctcc tcggccagca cgaggctctt
     1261 gtcgcacagc gatgccagca gatccgggac atcctgcagc tgcacgccgt cgccggcgca
     1321 gacttgcgcc gcggcggctt cggtccaccc ttcggcaaag accgacagcc tctgcagcgc
     1381 gagccgttcc ggctcctgca gcaggtcgta gctccagtcg atcagcgaac gcagggtctg
     1441 ctggcgcggc aaggccgtgc gcgaaccgcc cgtcagcagg aggaagcgct ggtcgagccg
     1501 gcggttgatc tcctcggccg acagcgaccg catgcgggcc gcggcgagct cgatggccag
     1561 cggtatgccg tccagccggc ggcagatgga ggcgatcgac ggggcgttcc tgtcggtgag
     1621 ccggaagtcc gggcgcacga gcgcggcccg atcgacgaag agctgcaccg cctcggactg
     1681 ggcgaccgac tcggaggtgg ccttccgatc cggttcgggc agcgcgagcg aaggcacgcg
     1741 aaacacctgc tcgccgccga tgccgagcgc ttcgcggctg ctcgccagca tcttcacctg
     1801 cgggcaagcc cgcaacaatg cttcggccag tggcgcgcag gcgtcgagca ggtgttcgca
     1861 gttgtccagc aggagcagag cctgccggtc cttgagatgt tcggcaagcg tctgcgtgat
     1921 cggcttgccc ggtacctcct tcaatcccag cacggttgcc acggtctgcg gcacgagctt
     1981 cggatcgacg agcggcgcga gctcggcgac ccacgcacca tcgggaaatt cgcccagcac
     2041 ggcgcccgcc agctgcagga ggaggcgcgt cttgccgacg ccgccgctgc cgtacagcgt
     2101 cagcagccgc gcctgcgaga ggcgctgcaa cagctcgtcg agttcacgcg cgcggccaac
     2161 gaacgacgcc acctgctccg gcaggttgtt gtcgatctgc cggaccggca cccacaacga
     2221 gtccttgcgg gcgacgcggt acaccttcgc gctgtcttcc ggcggcgcga agaccgtgtc
     2281 cgccgtgccg acctcgaaca gctcgatcgg ctcggcaaag cccttcatgc gccagtgccc
     2341 atgcgactgc acccgcaacg gcgtgtcccc gagcgcagcc agggcctcgc gcgtgagcag
     2401 cgtctggccg ccccgcgcaa gcgacatcac ccgcgcggcg gccgccttgg ccacaccctc
     2461 gacttcgatc ggcttggcac cgagcgcgac atcggcaggg ctgttttcgc gcagcgtgac
     2521 g
//
LOCUS       DASRDG010000151         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1054871, whole genome shotgun sequence.
ACCESSION   DASRDG010000151 DASRDG010000000
VERSION     DASRDG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054871"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..69)
                     /gene="rplV"
                     /locus_tag="VFA35_02425"
     CDS             complement(<1..69)
                     /gene="rplV"
                     /locus_tag="VFA35_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081245.1"
                     /note="binds specifically to 23S rRNA during the early
                     stages of 50S assembly; makes contact with all 6 domains
                     of the 23S rRNA in the assembled 50S subunit and ribosome;
                     mutations in this gene result in erythromycin resistance;
                     located near peptidyl-transferase center; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZT55055.1"
                     /translation="METKASLRGARLSAQKGRLVADM"
     gene            complement(199..465)
                     /gene="rpsS"
                     /locus_tag="VFA35_02430"
                     /pseudo
     CDS             complement(199..465)
                     /gene="rpsS"
                     /locus_tag="VFA35_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            complement(468..1295)
                     /gene="rplB"
                     /locus_tag="VFA35_02435"
     CDS             complement(468..1295)
                     /gene="rplB"
                     /locus_tag="VFA35_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZT55056.1"
                     /translation="MPLMKVKPTSPGRRALVKVVSPDLHKGEPHWPLVESQSRGSGRN
                     NHGRITVRHQGGGHKHHLRIIDFRRNKDGIAAKVEHLEYDPNRSAHLALLLYADGERR
                     YVLAAKGVQAGAQLMSGSEAPIKPGNALPLRNIPIGTEVHCVEMQPGKGAQLARSAGA
                     HVQVLAREGTYVQLRLRSGEIRKVHIDCRATIGEVGNAEHNLESIGKAGRVRWRGVRP
                     TVRGVAMNPIDHPHGGGEGRTGTAQPPVSPWGVQTKGYKTRKNKRTQAMIVRDRRVK"
     gene            complement(1299..1613)
                     /gene="rplW"
                     /locus_tag="VFA35_02440"
     CDS             complement(1299..1613)
                     /gene="rplW"
                     /locus_tag="VFA35_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009687194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZT55057.1"
                     /translation="MTAARKYNAEQLMNVVLAPVVSEKSTFVADKNRQYVFRVADRAT
                     KPQIKAAVELMFKTKVDGVTVLNVRGKERRFGRLMGRKRNWKKAYVRLAAGQEINFAA
                     TE"
     gene            complement(1610..2230)
                     /gene="rplD"
                     /locus_tag="VFA35_02445"
     CDS             complement(1610..2230)
                     /gene="rplD"
                     /locus_tag="VFA35_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZT55058.1"
                     /translation="MELKMVGNGGKGGGTVNGADAIFGREFNQALVHQLVVAYQANAR
                     LGTRAQKDRGAVNHSTKKPWRQKGTGRARAGMTSSPLWRGGGKIFPNSPEENFTQKVN
                     RKMYRAGLCAILSQLAREDRLRVVDEFRLEQPKTKLLAKKVKDMGFDEVLVITDEVDE
                     NLHLSARNLTNVHVIPVRYANPVWLVHSKNVLLTKAAVAKLEEMLK"
     gene            complement(2360..2531)
                     /locus_tag="VFA35_02450"
                     /pseudo
     CDS             complement(2360..2531)
                     /locus_tag="VFA35_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L3"
BASE COUNT          322 a          871 c          843 g          495 t
ORIGIN      
        1 catgtcggcg accagccggc ccttctgggc cgagaggcgc gcaccgcgca ggcttgcttt
       61 ggtttccatg tccattcctt atttcttcgc tggcgccgcc gcagcagccg ccggcgccgc
      121 agccggggcg gcagcaggcg cggctgcgcc cggcgctccc ggcggcccgc gccgatcgcc
      181 gccgaccgac tcctcggtgc gcttgccggc cgccgagtgg ctcttgaaga tgcgcgtcag
      241 cgcgaattcg ccgagcttgt ggccgaccat gttctccgtc acgtacaccg gcacgtgctg
      301 cttgccattg tggacggcaa tggtgaggcc cacgaaatcc ggcagtatgg tcgagcggcg
      361 cgaccaggtc ttgatcgggc gcttgtcgct ggtgctgcgc gccttgtcga ccttggcctg
      421 caggtgatgg tccacgaacg gacccttctt gattgagcga gccatggtta tttcaccctt
      481 ctgtcgcgga cgatcatcgc ctgcgtgcgc ttgttcttgc gcgtcttgta gcccttggtc
      541 tgcacgcccc acggcgacac cggcggctgc gcggtgccgg tgcggccttc gccgccgccg
      601 tgcgggtggt cgatcgggtt catcgccacg ccgcgcacgg tcgggcgcac gccgcgccag
      661 cgcacccgtc cggccttgcc gatcgactcg aggttgtgct cggcattgcc gacctcgccg
      721 atcgtggcgc ggcaatcgat atgcaccttg cggatctcgc ccgagcgcag gcgaagctgc
      781 acgtacgtgc cttcgcgcgc cagtacctgg acatgggcgc cggccgagcg cgcgagctgc
      841 gcgcccttgc cgggttgcat ctcgacgcag tgcacttcgg tgccgatcgg gatgttgcgc
      901 aacggcagcg cgttgcccgg tttgatcggc gcttcggagc cggacatgag ctgcgcgccg
      961 gcctgtacgc ccttcgccgc gagcacgtag cggcgctcgc catcggcgta gagcaacagc
     1021 gcgaggtgcg cgctgcggtt agggtcgtat tccaggtgct cgaccttggc ggcaatgccg
     1081 tccttgttgc ggcggaaatc gatgatgcgc aggtggtgct tgtgtccacc accctggtgg
     1141 cgcacggtga tgcggccatg gttgttgcgg ccgctgccgc gggactggct ctcgacgagc
     1201 ggccagtgcg gttcgccctt gtgcaggtcg ggcgagacca ccttgacgag cgcgcgccgg
     1261 cctggcgagg tcggtttgac tttcatcaat ggcatgggtt actccgtcgc cgcaaagttg
     1321 atttcctggc cggccgcgag tcgcacgtag gccttcttcc agttgcgctt gcggcccatg
     1381 agccggccga agcgccgctc cttgccgcgc acgttgagca cggtgacgcc gtcgaccttg
     1441 gtcttgaaca tgagctcgac cgccgccttg atctgcggct tggtggcgcg gtcggcgacg
     1501 cggaacacgt actggcgatt cttgtcggcg acgaaggtgc tcttctccga caccaccggc
     1561 gccagcacca cgttcatcag ctgctcggcg ttgtacttcc gggccgcagt catttgagca
     1621 tctcctcgag cttcgccacg gctgccttcg taagcagcac attcttcgag tgcaccagcc
     1681 acaccgggtt ggcatagcgc acgggaatca cgtgcacgtt cgtcaggttg cgcgcggaaa
     1741 gatgcaggtt ctcatccacc tcgtcggtga tcaccaggac ttcgtcgaag cccatgtcct
     1801 tcaccttctt cgcgagcagc ttggtcttgg gctgctcgag gcggaactcg tcgaccacgc
     1861 gcagccggtc ctcgcgcgcg agctgcgaca ggatcgcgca caggcccgcg cggtacatct
     1921 tgcggttcac cttctgcgtg aaattctcct ccggcgagtt cgggaagatc ttgccgccgc
     1981 cgcgccagag cgggctcgag gtcatgccgg cgcgcgcccg gccggtgcct ttctgccgcc
     2041 agggcttctt ggtggagtga ttgaccgcgc cgcggtcctt ctgcgcccgc gtaccgagac
     2101 gcgcgttcgc ctgatacgcg accaccagct ggtgtaccag cgcctgattg aattcgcggc
     2161 cgaagatggc gtccgccccg ttgacggtgc cgccgccctt gccgccgttg ccgaccattt
     2221 tcagttccat gtcggcccct tatttcttcg gctgcgctgc aggcttcgcc gcgggcttgg
     2281 cggcgggctt gggctcgggc ctgggcgcgg cgggcttcgc ctgcgccgcc ggcttcgcct
     2341 gctgcaccgg cgccttgtag agcttcgccg ccggacgcac gaccacgtcg cggcccttgg
     2401 cgccgggcac cgagcccttc accagcagga gctggcgctc ggcgtcgaca cgcaccaccg
     2461 tcaggcccga gatggtgcgc tgcttatcgc cgagatggcc cggcatgcgc ttgcccggga
     2521 aaacatgcgc c
//
LOCUS       DASRDG010000152         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1407825, whole genome shotgun sequence.
ACCESSION   DASRDG010000152 DASRDG010000000
VERSION     DASRDG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407825"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VFA35_02455"
     CDS             complement(<1..842)
                     /locus_tag="VFA35_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZT55059.1"
                     /translation="MTTDYDKLDAWIDAHFDEEVKFLQALVQVPTDTPPGNNAPHAER
                     TAELLRGFGLEAEKHVVPDAEVKAYGLASLTNLIVRRPYGAGRTIALNAHGDVVPPGD
                     GWAHDPYGGEVIDGRLYGRASAVSKSDFATYTFAVRAIETLKSQLKGAVELHFTYDEE
                     FGGELGPGWLLRHKLTRPDLLLAAGFSYQVVTAHNGCLQMEVTVHGKMAHAAIPDTGV
                     DALQGAVAILNALYHQNTLYKQVTSKVEGITHPYLNVGRIEGGTNTTVVPGKVVLKLD
                     RRMIP"
     gene            complement(854..>2419)
                     /locus_tag="VFA35_02460"
     CDS             complement(854..>2419)
                     /locus_tag="VFA35_02460"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HZT55060.1"
                     /translation="AGKAMVAKTLTAESTHEQGRAGLAECTPAEFAHLQALNAAYNEK
                     FGWPFTVAVRGPRGSGLTRHEIIKTFERRLAGHPDFELAECVRNIHRIAELRLNDKFR
                     VEPTLGHQAWDCAELLARHTDPGYGERNELTVTYLTDAHRACAAQLQAWMLECGFDEA
                     KIDAVGNVVGLYHGSDPGAKRLLTGSHYDTVRNGGKYDGRLGIFVPMICVRELHRAGK
                     RLPYGFEVVGFAEEEGQRYKATFLGSGALTGHFDPAWLDQRDADGVTMLDAMRLAGLP
                     ATLPAIRALQRDPGAYQGFVEVHIEQGPVLNALDLPLGVVTSINGSVRYLGEVVGMAS
                     HAGTTPMDSRRDAATAVAELALYLEKRAGAAPNLVGTMGILTVPNGSTNVVPGRCQFT
                     LDIRATTNEVRDACAADVLAELARICERRGLAHTIEETMRAAAAPSAPEWQQRWERAV
                     AAAGLPVFRMPSGAGHDAMKLHEAMPQAMLFVRGQNAGISHNPLESSTSDDMQLAVDS
                     FMHVLNQLATEST"
BASE COUNT          322 a          812 c          871 g          414 t
ORIGIN      
        1 gggatcatgc ggcggtcgag cttgagcacg accttgccgg gcacgacggt ggtgttggtg
       61 ccgccctcga tgcggccgac attgaggtac gggtgcgtga tgccctcgac cttggaggtg
      121 acctgcttgt acagcgtgtt ctggtggtac agcgcattca ggatcgcgac cgcgccctgc
      181 agcgcgtcga cgccggtgtc ggggatcgcg gcgtgtgcca tcttgccgtg caccgtgact
      241 tccatctgca ggcagccgtt gtgcgcggtc acgacctggt agctgaagcc ggcggcgagc
      301 agcaggtcgg gccgggtgag cttgtggcgc agtagccagc cggggccgag ctcaccgccg
      361 aactcttcgt cgtaggtgaa gtgcagctcg accgcgccct tcagctgcga cttcagcgtc
      421 tcgatcgcgc gcaccgcgaa ggtgtaggtc gcgaagtcgc tcttgctgac ggccgaggcg
      481 cggccgtaga gcctgccgtc gatcacctcg ccaccgtagg gatcgtgggc ccaaccgtcg
      541 ccgggcggca cgacgtcgcc gtgcgcgttc agcgcgatcg tcctgcctgc gccatagggc
      601 cggcgcacga tcaggttggt cagcgatgcg agaccgtagg ccttgacctc ggcgtcgggc
      661 accacgtgct tctcggcctc taagccgaag ccgcgcaaca gctcggccgt gcgctcggca
      721 tgcggcgcgt tgttgccggg cggtgtgtcg gtcggcactt gcacgagcgc ctgcaggaac
      781 ttcacttcct cgtcgaagtg tgcgtcgatc caggcgtcga gcttgtcgta gtcggttgtc
      841 atcgggaagt ctttcaagtg gattcggtcg ccagctgatt cagcacatgc atgaacgaat
      901 cgaccgcaag ctgcatgtcg tcgctggtgc tcgattcgag cgggttgtgg ctgatgccgg
      961 cgttctggcc gcgcacgaac agcatcgcct gcggcatcgc ctcgtgcagc ttcatcgcgt
     1021 cgtggccggc gccgctgggc atgcggaaca ccggcagccc ggcggcggcg accgcgcgct
     1081 cccagcgctg ctgccactcg ggcgcgctcg gtgcggcggc ggcgcgcatc gtctcctcga
     1141 tcgtgtgagc cagcccgcgg cgttcgcaga tgcgcgccag ctcggccagc acgtcggccg
     1201 cgcaggcgtc gcgcacctcg ttggtggtgg cgcggatgtc gagcgtgaac tggcagcggc
     1261 cgggcacgac gttggtcgag ccgttcggca ccgtcaggat gcccatcgtg ccgacgaggt
     1321 tcggcgccgc gccggcgcgc ttttccagat acagcgcgag ctcggcgacc gcggtcgcgg
     1381 cgtcgcgccg gctgtccatc ggcgtggtgc cggcgtggct ggccatgccg accacctcgc
     1441 cgaggtagcg cacgctgccg ttgatcgacg tgaccacgcc cagcggcagg tcgagcgcat
     1501 tgagcaccgg gccctgctcg atgtggacct cgacgaagcc ctggtacgcg cccgggtcgc
     1561 gctgcagcgc gcggatcgcc ggcaaggtcg cgggcaggcc ggccaggcgc atcgcatcga
     1621 gcatcgtgac gccgtcggca tcacgctgat cgagccaggc ggggtcgaag tggccggtca
     1681 gcgcgcccga gccgaggaag gtcgccttgt agcgctggcc ttcctcctcg gcgaagccga
     1741 cgacctcgaa gccgtagggc aggcgcttgc cggcacggtg cagctcgcgc acgcagatca
     1801 tcggcacgaa gatgccgagc cgcccgtcgt acttgccgcc gttgcgcacc gtgtcgtagt
     1861 gcgagccggt cagcagccgc ttggcgccgg ggtcgctgcc gtgatagagg ccgacgacat
     1921 tgccgaccgc gtcgatcttt gcttcatcga agccgcattc gagcatccac gcctgcagct
     1981 gcgcggcgca ggcgcggtgc gcgtcggtca ggtaggtgac ggtgagctcg ttgcgctcgc
     2041 cgtagcccgg gtcggtgtgg cgcgccagca gctcggcgca gtcccacgcc tggtggccca
     2101 gagtcggctc gaccctgaac ttgtcgttca ggcgcagctc ggcgatgcgg tggatgttgc
     2161 gcacgcactc ggcgagctcg aaatcggggt ggcccgccag gcggcgctcg aaggtcttga
     2221 tgatctcgtg ccgcgtcagc ccgctgccgc gcggcccgcg caccgcgacc gtgaacggcc
     2281 agccgaactt ctcgttgtag gcagcgttca gcgcctgcag gtgcgcgaac tcggccggcg
     2341 tgcactcggc gagtccggcg cgcccttgct cgtgcgtcga ctcggcggtc agcgtcttcg
     2401 cgaccatcgc cttgccggc
//
LOCUS       DASRDG010000153         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_382982, whole genome shotgun sequence.
ACCESSION   DASRDG010000153 DASRDG010000000
VERSION     DASRDG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382982"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFA35_02465"
     CDS             <1..351
                     /locus_tag="VFA35_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55061.1"
                     /translation="QARAFYEGLLGLRELRDPATDRPGALHYALGWGRLDLSEGHYTG
                     VAPQAHLALRVTGLAEITRRLRWAGIAVDAAPLFDAGRIYVVDPFGNRLELIEPGAAD
                     DPENRHVSAYRFSA"
     gene            323..1138
                     /gene="ssuC"
                     /locus_tag="VFA35_02470"
     CDS             323..1138
                     /gene="ssuC"
                     /locus_tag="VFA35_02470"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HZT55062.1"
                     /translation="MSAPTASRPERRGAPWAAALLPFALPLALLIAWELSSRLGWLST
                     RVLPEPLAVVRAFWALAVSGEIWRHVAISSARALGGFAIGGGLGLVLGLLTGSLRSAE
                     TLLDSTLQMLRNIPALALIPLVILWFGIDESAKLFLVATGVFFPVYINTFHGIRAVDK
                     GLIEMARSYGLQGWPLYRQVILPGALPSILVGVRFSLGLMWVILIVAETISAQAGIGY
                     MTMNAREFLQTDVVLVGILLYALLGKLADLLSKGLERWLLRWHPGYRPQELRA"
     gene            1135..1980
                     /locus_tag="VFA35_02475"
     CDS             1135..1980
                     /locus_tag="VFA35_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT55063.1"
                     /translation="MKNPAALRLLPSHAVTGARVEARGLAKSYGARAVLRDVDLRIAP
                     GEFVALVGRSGCGKSTLLRLLAGLEAVDAGRIAFDGATQAAAPDGHRADTRIMFQDAR
                     LLPWKRVRDNVALGLSSKDAAQRALDALAQVGLADRADDWPAVLSGGQRQRVALARAL
                     VHTPRLLLLDEPLGALDALTRIEMQRLIEGLWQRHGFTAVLVTHDVGEAVALADRVLL
                     IEDHRIALDQRVALPRPRSRGQAAFAQHEERLLERVLQQPATDTAEDTPDAPPVAAAA
                     LRWAV"
     gene            2010..2228
                     /locus_tag="VFA35_02480"
     CDS             2010..2228
                     /locus_tag="VFA35_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZT55064.1"
                     /translation="MGITAINVRNQFRGKVKEIIEGSVVSEIDVETPSGLIVTSVITT
                     RSVRELGLAPGRDVIALVKSTEVSIATL"
     gene            2335..>2730
                     /locus_tag="VFA35_02485"
     CDS             2335..>2730
                     /locus_tag="VFA35_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT55065.1"
                     /translation="MTRLLIVPGLHDSGDAHWQTDLQRHHRHARRVQQHDWSVPDLEA
                     WADHVLDTLAAEPGGHEKAPWLAVAHSFGCLALARALLRGAPGIAAAVLVAPADPLKF
                     GVAESLPCRPLPVPSTLVASRTDPWMPFGS"
BASE COUNT          341 a         1011 c          982 g          396 t
ORIGIN      
        1 caggcgcgcg cgttctacga aggcctgctc ggcctgcgcg agctgcgcga cccggcgacg
       61 gaccggcccg gcgcgctgca ctacgcgctc ggctgggggc gcctggacct gagcgaaggc
      121 cactacacgg gcgtcgcgcc gcaggcgcac ctcgcgctgc gcgtcaccgg cctggccgag
      181 atcacgcggc ggctgcgctg ggccggcatc gcggtcgatg ccgcgccgct gttcgacgcc
      241 ggtcgcatct acgtcgtcga cccgttcggt aaccggctcg aactgatcga acccggtgcc
      301 gccgatgacc cggagaatcg ccatgtcagc gcctaccgct tctcggcctg agcgccgcgg
      361 cgcaccgtgg gcggcggcgc tgctgccgtt cgcgttgccg ctcgcgctgc tgatcgcgtg
      421 ggagctgtcg tcccggctcg gctggctctc gacgcgcgtg ctgcccgagc cgctcgcggt
      481 cgtgcgcgcg ttctgggccc tcgcggtctc gggcgagatc tggcggcacg tcgcgatcag
      541 cagcgcgcgc gcgctcggtg gcttcgcgat cggcggcggg ctcgggctgg tgctgggcct
      601 gctcaccggc tcgctgcgca gcgccgagac gctgctcgac tcgaccctgc agatgctgcg
      661 caacatcccg gcgctcgcgc tgatcccgct cgtgatcctg tggttcggca tcgacgagtc
      721 ggcgaagctg ttcctggtcg cgaccggcgt gttcttcccg gtctacatca acaccttcca
      781 cggcatccgc gcggtcgaca aggggctgat cgagatggcg cgcagctacg ggctgcaggg
      841 ctggccgctg taccgccagg tgatcctgcc gggcgcgctg ccgagcatcc tggtcggcgt
      901 gcgcttctcg ctcgggctga tgtgggtgat cctgatcgtc gccgagacga tctcggcgca
      961 ggccggcatc ggctacatga cgatgaacgc gcgcgagttc ctgcagacgg acgtggtgct
     1021 cgtcggcatc ctgctgtacg cgctgctcgg caagctcgcc gacctgctct cgaaggggct
     1081 ggagcgctgg ctgctgcgct ggcacccggg ctatcgaccg caggagctgc gcgcatgaag
     1141 aaccccgcag cgttgcgcct gctgccgtcg cacgccgtca cgggcgcgcg ggtcgaggcg
     1201 cggggcctgg ccaagtcgta cggtgcgcgc gcggtgctgc gcgatgtgga cctgcgcatc
     1261 gcgcccggcg agttcgtcgc gctggtcggc cgcagcggct gcggcaagag caccttgctg
     1321 cgcctgctcg ccgggctcga ggccgtcgac gccggccgca tcgcgttcga tggcgcgacc
     1381 caggctgcgg cgcccgacgg ccaccgcgcc gacacccgca tcatgttcca ggacgcgcgg
     1441 ctgctgccgt ggaagcgcgt gcgcgacaac gtcgcgctcg gcctgagctc gaaggacgcg
     1501 gcgcagcgcg cgctcgatgc gctcgcccag gtcggcctgg ccgatcgcgc cgacgactgg
     1561 ccggccgtgc tctcgggtgg ccagcgccag cgtgtcgcgc tggcgcgcgc gctggtgcac
     1621 acgccgcgcc tgctgctgct cgacgagccg ctgggcgcgc tcgacgcgct cacgcgcatc
     1681 gagatgcagc gcctgatcga gggcctctgg caacgccacg gcttcaccgc ggtgctggtc
     1741 acccacgatg tcggcgaggc ggtcgcgctg gccgaccgcg tgctgctgat cgaagaccac
     1801 cgcatcgcgc tcgaccagcg cgtcgcgctg ccgcggccgc gctcgcgcgg gcaggccgcg
     1861 ttcgcgcagc acgaggagcg cctgctcgag cgcgtgctgc agcaaccggc caccgacacg
     1921 gccgaagaca cccccgacgc accgcccgtc gccgccgccg cgctgcgctg ggccgtctga
     1981 cgcatcacct cacgaaagga atcgacccca tgggcatcac cgccatcaac gtgcgcaacc
     2041 agttccgcgg caaggtcaag gagatcatcg aggggtcggt ggtcagcgag atcgacgtcg
     2101 agacaccctc gggcctgatc gtcacctcgg tgatcacgac gcgctcggtc agggaactcg
     2161 gcctcgcgcc cggccgcgac gtgatcgcgc tggtcaagtc gaccgaagtc tcgatcgcga
     2221 cgctgtgaat accagaacca atgcggcgcg cgcgggccga gcgccgggcc gccccaagcc
     2281 ggcccgcatc cccccggggg atcgaccgac gtactcgtcg ggcgaggggc tgtcatgaca
     2341 cgcctgttga tcgtgccggg gctgcacgac agcggcgacg cgcattggca gaccgacctg
     2401 cagcgccacc accgccacgc gcgccgcgtg cagcagcacg actggagcgt gcccgacctc
     2461 gaagcctggg ccgaccacgt gctcgacacg ctcgccgctg agcctggcgg gcatgagaag
     2521 gcgccctggc tcgcggtcgc gcacagcttc ggctgcctgg cgctcgcgcg ggcgctgctg
     2581 cgcggcgcgc cgggcatcgc cgccgcggtg ctggtcgcgc ccgccgaccc gctcaagttc
     2641 ggcgtcgccg agtcgctgcc gtgccggccg ttgccggtgc cgagcacgct ggtcgcgagc
     2701 cgcaccgacc cgtggatgcc gttcggctcg
//
LOCUS       DASRDG010000154         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1286407, whole genome shotgun sequence.
ACCESSION   DASRDG010000154 DASRDG010000000
VERSION     DASRDG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286407"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1324
                     /locus_tag="VFA35_02490"
                     /pseudo
     CDS             1..1324
                     /locus_tag="VFA35_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347028.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
     gene            1329..1862
                     /locus_tag="VFA35_02495"
     CDS             1329..1862
                     /locus_tag="VFA35_02495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT55066.1"
                     /translation="MTAPPAVTIRRASVKDAAAMARYMSDPVIFGGLLQMPYPSEEMW
                     HARLSDGAAAVKPDLSLVAERDGAVIGSAGLFSPGPAPRRRHAMGLGISVATAAHRQG
                     VGTALMAALCDYADNWLGCLRIELTVYTDNEVAQRLYRNFGFELEGTFRGYALRDGAF
                     VDAHSMARLHPNPPAIA"
     gene            1864..>2259
                     /locus_tag="VFA35_02500"
     CDS             1864..>2259
                     /locus_tag="VFA35_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55067.1"
                     /translation="MRAWCWAIAMLWAPLVQAQERVALPSLDRSGGVPVELIGFWFGA
                     SMPAPAPAVVLLHGCGGTYDAKGRLSQRTLDYARLLNERGMHALVVDSLTPRGEKELC
                     TQRVGTRRVTQANRRLDALAAVDWLAHRPG"
BASE COUNT          346 a          757 c          851 g          305 t
ORIGIN      
        1 ggctacgagg agctcgtgat cgagcgctcc acggtcacgg cgctgtacgt cgacggctcg
       61 ccggtgccgc gcgccggcgc gggcgacgat gtcgtcgtgg tgctcgatcg cacgccgttc
      121 tacgccgaga gcggtggcca gcgcgaaggc cggcgacgat gcggtgatcg tgctcgacca
      181 cacgccgttc tatgccgagt ccggcggcca ggtcggcgac accggcgagc tgcgcaacgc
      241 cagctcgcgc ttcctcgtcg aggacacgat caaggtgcag ccggcggtgt tcggccacca
      301 cggccgcgtc gtcgagggcg agatcaaggt cggcgacgtg ttcaccgcca aggtcgacgc
      361 cgagcagcgc gcgaagaccg tgcgcaacca ctcgtcgacg cacctgatgc acaaggcgct
      421 gcgcgaagtg ctgggcgatc acgtgcagca gaagggctcg ctcgtcaacg ccgagcgcac
      481 gcgcttcgac ttcgtgcaca gcgggccgct gaccgaagac cagattgcca aggtcgaggc
      541 gctcgtcaac gccgagatcc tcgcgaacgc cgccacgcag gcgcgggtga tgccgatcga
      601 cgaggcgcag aagtcgggcg cggtgatgct gttcggcgag aaatacggcg acgaggtgcg
      661 cgtgctcgac atcggcagca gccgcgagtt ctgcggcggc acgcacgtcg cgcgcaccgg
      721 cgacatcggc ctgttcaaga tcgtcgccga aagcggcgtg gccgccggtg tgcggcgggt
      781 cgaggcgatc accggcgaca acgcgctggc ctacctgcag tcgctcgaag gcacggtgca
      841 gcagctggcc ggcgcgttga aggcggcgcc cgccgaagtg ccggcgcgcc tgacgcaggt
      901 gctcgaccac gtgcgcgcgc tcgagaagga gctcgcggcg ctgaagggca agctggcgtc
      961 gtcgcaaggc gacgagctga tggccggcgc cgtcgacgtg aacggcatca aggtgctggc
     1021 cgcgatgctg gccggcgccg acgccaagac gctgcgcgac acgctggaca agctgaagga
     1081 caagctgaag accgccgcga tcgtgctggc gaccgtcgaa ggcggcaagg tgcagatcgc
     1141 cgccggcgtg acgagcgaca gcattggcaa gctcaaggcc ggcgaactcg tcaacttcgt
     1201 cgcgcagcag gtcggcggca agggcggcgg caagcccgac atggcgatgg ccggcggcac
     1261 cgacccgagc aagctcgacg cggcgctcgc gtcggtgcgc ggctgggtgg ccgagcgggt
     1321 gtgaccgcat gaccgcgccg cccgccgtga cgatacgccg cgcgagcgtc aaggacgcgg
     1381 cggcgatggc gcgctacatg agcgaccctg ttatcttcgg cgggctgctg cagatgccgt
     1441 acccgagcga ggagatgtgg catgcgcgct tgtccgacgg cgcggcggcg gtcaagcccg
     1501 acctgtcgct ggtcgccgaa cgcgacggcg cggtgatcgg ctcggccggc ctgttcagcc
     1561 ccggcccggc gccgaggcgc cggcatgcga tgggactggg catctcggtc gcgacggcgg
     1621 cgcaccggca gggcgtgggc accgcgctga tggcggcgct gtgcgactac gccgacaact
     1681 ggcttggctg cttgcgcatc gagctgacgg tctacaccga caacgaggtg gcgcaacggc
     1741 tgtaccgcaa cttcggcttc gagctcgaag gcacgttccg cggctacgcg ctgcgcgatg
     1801 gcgcgttcgt cgacgcgcac tcgatggcac gcctgcaccc gaacccgccg gctatcgcct
     1861 gagatgcgcg cttggtgctg ggcgatcgcg atgctgtggg cgccgctggt gcaggcgcag
     1921 gagcgcgtcg ccttgcccag cctggatcgc agcggcggtg tgccggtcga gctgatcggc
     1981 ttctggttcg gcgcgtcgat gccggccccc gcgcctgccg tcgtgctgtt gcacggctgc
     2041 ggcggcacct acgacgccaa gggccggctg agccagcgca cgctggacta cgcgcggctg
     2101 ctgaacgagc gcggcatgca tgcgctggtc gtggactcgc tgacgccgcg cggcgagaag
     2161 gagctgtgca cgcagcgcgt cggcacgcgc cgtgtgacgc aggcgaatcg ccggctggat
     2221 gcgctcgccg ccgtcgactg gctcgcgcat cgccccggc
//
LOCUS       DASRDG010000155         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_383280, whole genome shotgun sequence.
ACCESSION   DASRDG010000155 DASRDG010000000
VERSION     DASRDG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383280"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VFA35_02505"
     CDS             complement(<1..1189)
                     /locus_tag="VFA35_02505"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZT55068.1"
                     /translation="MVDKRNFSFADLENWFNEHRVTEIECLVPDLTGVARGKILPREK
                     FTEDRGMRLPEAIVGIGVTGNFPTDGPYYDVIHPTDRDMHLRPDPSTVRIVPWAADPT
                     AQVIHDCFDRDGQMIPYAPRSVLRRICELYAAEGWEPVVAPELEFYLIERNADPNTPL
                     RPPRGRSGRAETSRQAYSIDAVNEFDPLFEDIYDYCGKMELNVDTLIHEVGAGQMEIN
                     FFHDHPLGLADEVFFFKRTIREAALRHEMYATFMAKPMAGEPGSAMHVHQSILNKITG
                     KNIFSNDDGSPSKAFYWYIGGLQKYTPAAMALFAPYVNSYRRLARSTAAPINIQWGTD
                     NRTVGIRSPVATAAARRIENRVIGADANPYVALAATLACGYLGMKNKIEPGPECKGDA
                     YLSE"
     gene            complement(1303..2088)
                     /locus_tag="VFA35_02510"
     CDS             complement(1303..2088)
                     /locus_tag="VFA35_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZT55069.1"
                     /translation="MPTAPSSRPRPVVLVPACNRVLGDHPFHVAGKKYVDAVRLAGCT
                     PLIVPGASGDELDALLGLADGVLLTGSPSNVHPRHFAEDVHDASLPLDADRDAWTLPL
                     IPRALERGIPLFAICRGFQEMNVALGGSLHQAVQEVPDRHDHRAAKNAPAEAQYGPAH
                     DLHVQPGGVLARVLDAPTIRVNSVHGQGVNRLARGLRIEALAPDGLVEAFSADAARGF
                     NLGVQWHPEWQAETNPVSIRLLEAFGAACVAYRDRHRKPEPDR"
     gene            complement(2113..3408)
                     /locus_tag="VFA35_02515"
     CDS             complement(2113..3408)
                     /locus_tag="VFA35_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZT55070.1"
                     /translation="MDAYWMPFTANRQFKKAPRLLAKAQGMHYWTPEGRQILDGVAGL
                     WCVNAGHARPKIVEAIAAQAAEMDFAPPFNMAHPKAFELAERLVELTPAGLNKVFFAN
                     SGSEAVETALKIAIGYHRARGEGARTRLIGRERGYHGVNFGGTTVGGIVGNRKTFGPL
                     LPGTDHIRHTHDLARNAFARGLPEHGAELAEDLERLVALHDASTIAAVIVEPVAGSTG
                     VLLPPKGYLKRLREICDKHGILLIFDEVITGFGRVGSPFAAQQFDVAPDMMTVAKGIT
                     NGCVPMGAVFARQAIHDVFMTGPEHLIEFPHGYTYSAHPLACAAALGTLDTYAEEGLL
                     TRAATMAPVFENALHSLRGVPHVIDVRNIGLVGGIELEPVAGQPGKRAFDVFLECWER
                     GVLIRTTGDTIALSPPLIVESAHIDQMVSTIADVLKKNA"
     gene            3643..4389
                     /locus_tag="VFA35_02520"
     CDS             3643..4389
                     /locus_tag="VFA35_02520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZT55071.1"
                     /translation="MESSRPVVVLQNLSADGPGYLGTWLAEQGIAFDVFDAEAGNPYP
                     WSIAGYRALALLGGEMSANDDLPSLRQAERLILQAIDADIPVIGHCLGGQLMARALGA
                     RVIASPKPEIGWQAMQVADTPAARRWFGEPGERIVYHWHGESFELPAGAELLAGNASC
                     PHQAFAIGRHLALQFHVELDACKLAAWSASCDPSFVRLQREYPATVQTGAAMRAQAGT
                     ALPAQLRLADRMYGRWLGIASPARFHDRGP"
     gene            4616..5254
                     /gene="aceA"
                     /locus_tag="VFA35_02525"
                     /pseudo
     CDS             4616..5254
                     /gene="aceA"
                     /locus_tag="VFA35_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009931004.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT          787 a         1738 c         1872 g          857 t
ORIGIN      
        1 actccgacag gtaggcgtcg cccttgcact cgggacccgg ctcgatcttg ttcttcatgc
       61 ccaggtagcc gcaggcgagc gtggcggcga gcgcgacgta cggattggcg tcggcgccga
      121 tcacgcggtt ctcgatgcgc cgtgccgcgg cggtcgcgac cggcgagcgg atgcccaccg
      181 tgcggttgtc ggtgccccac tggatgttga tcggcgccgc ggtcgagcgc gccagccgcc
      241 ggtacgagtt gacgtagggc gcgaacagcg ccatcgccgc cggcgtgtac ttctgcagcc
      301 cgccgatgta ccagtagaac gccttgctcg gcgagccgtc gtcgttgctg aagatgttct
      361 tgccggtgat cttgttcagg atgctctggt gcacgtgcat cgcgctgccc ggctcgccgg
      421 ccatcggctt ggccatgaag gtcgcgtaca tctcgtggcg cagcgcggcc tcgcggatgg
      481 tgcgcttgaa gaagaacacc tcgtcggcca ggcccagcgg gtggtcgtgg aagaagttga
      541 tctccatctg ccccgcgccg acctcgtgga tcaacgtgtc tacgttcagc tccatcttgc
      601 cgcagtagtc gtagatgtcc tcgaacagcg ggtcgaactc gttgaccgca tcgatcgagt
      661 aggcctggcg cgaggtctcg gcgcggccgc tgcggcccct gggcgggcgc agcggcgtgt
      721 tcggatcggc gttgcgctcg atcaggtaga actcgagctc gggcgcgacg accggctccc
      781 agccctcggc cgcatacagc tcgcagatgc ggcgcagcac cgagcgcggt gcgtacggga
      841 tcatctgccc atcgcggtcg aagcagtcgt ggatcacctg tgcggtcggg tcggcggccc
      901 agggcacgat gcgcacggtg ctcgggtcgg gccgcaggtg catgtcgcgg tcggtcgggt
      961 ggatcacgtc gtagtacggt ccgtcggtcg ggaagttgcc ggtcacgccg atgccgacga
     1021 tcgcttcggg cagccgcatg ccgcggtctt cggtgaactt ctcgcgcggc aggatcttgc
     1081 cgcgcgccac gccggtcagg tcgggcacga ggcactcgat ctcggtcacg cggtgttcgt
     1141 tgaaccagtt ctcgaggtcg gcgaaggaga agtttcgttt gtccaccatg gggcacctgt
     1201 tgcgtgggcg cgttcgtcgc gtgacgctgc gccgggatcg atcatcgcgt ctcgggcgtg
     1261 gcgccaggcc cgaagcccgg gccgaccacg caagacgcgc agctatcgat cgggctcggg
     1321 cttgcggtgc cggtcgcggt acgccacgca ggcggcgccg aaggcctcga gcaggcggat
     1381 cgacaccggg ttcgtctcgg cctgccactc ggggtgccat tgcacgccga ggttgaagcc
     1441 gcgggccgcg tcggcagaga aggcctcgac caggccgtcg ggcgcgagcg cctcgatgcg
     1501 caggccgcgc gcgagccggt tcacgccctg gccatggacc gagttgacgc ggatggtcgg
     1561 tgcgtcgagc acgcgcgcga gcacgccgcc cggctgcacg tgcaggtcgt gcgcgggccc
     1621 gtactgcgct tcagcgggcg cgttcttcgc ggcgcggtgg tcatgccggt ccggcacttc
     1681 ctgtaccgcc tggtgcagcg agccgccgag cgccacgttc atctcctgaa acccgcggca
     1741 gatcgcgaac agcgggatgc cgcgttcgag cgcgcgcggg atcagcggca gcgtccaggc
     1801 atcgcgatcg gcgtcgagcg gcagcgacgc gtcgtgcaca tcctcggcga aatgacgcgg
     1861 gtgcacgttc gatggcgagc cggtcagcag cacgccgtcg gccaggccga gcagcgcgtc
     1921 gagctcgtcg ccggacgcgc ccggcacgat cagcggcgtg cagccggcga ggcgcaccgc
     1981 gtcgacgtat ttcttgccag cgacatggaa agggtgatcg cccagcacgc ggttgcacgc
     2041 gggcacgagc accaccgggc gagggcggga ggagggtgct gtcggcatgc cccgcagcat
     2101 accgtggccc cgtcaggcgt tcttcttcag cacgtctgcg atcgtgctga ccatctggtc
     2161 gatgtgcgcg ctctcgacga tcagcggcgg cgacagcgcg atcgtgtcgc cggtggtgcg
     2221 gatcagcacg ccgcgctccc agcattcgag gaagacatcg aacgcgcgct tgcccggctg
     2281 gccggccacg ggctcgagct cgatgccgcc gaccaggccg atgttgcgca cgtcgatcac
     2341 gtgcggcacg ccgcgcagcg agtgcagcgc gttctcgaac accggcgcca tggttgcggc
     2401 gcgggtcagc aggccctctt cggcataggt gtcgagcgtg ccgagcgcag cggcgcaggc
     2461 gagcgggtgc gccgagtagg tgtagccgtg cgggaactcg atcaggtgct ccgggccggt
     2521 catgaagacg tcgtgtatcg cctgcctcgc gaacaccgcg cccatcggca cgcagccgtt
     2581 ggtgatgccc ttggcgacgg tcatcatgtc gggcgccacg tcgaactgct gcgccgcgaa
     2641 cggcgacccg acgcggccga aaccggtgat cacctcgtcg aagatcagca ggatgccgtg
     2701 cttgtcgcag atctcgcgca ggcgcttcag gtagcccttc ggcggcagca gcacgccggt
     2761 cgagccggcg accggctcga cgatcaccgc ggcgatcgtc gaggcgtcgt gcagcgccac
     2821 caggcgctcc agatcctcgg ccagttcggc gccgtgctcg ggcaggccgc gcgcgaaggc
     2881 attgcgggcg aggtcgtggg tgtggcggat gtggtcggtg cccggcagca gcgggccgaa
     2941 ggtcttgcgg ttgccgacga tgccgccgac cgtggtgccg ccgaaattca cgccgtggta
     3001 gccgcgctcg cggccgatca gccgggtgcg cgcgccttcg ccgcgggcgc ggtggtagcc
     3061 gatcgcgatc ttcagcgcag tctcgacggc ctccgagccg gagttggcga agaagacctt
     3121 gttcaggccg gccggcgtca gctcgaccag gcgctcggcc agctcgaagg ccttcgggtg
     3181 cgccatgttg aacggcggcg cgaagtccat ctcggccgcc tgcgccgcga tcgcctcgac
     3241 gatcttcggc cgcgcgtggc cggcgttgac gcaccacagg cccgccacgc cgtcgaggat
     3301 ctggcggccc tcgggcgtcc agtagtgcat gccttgcgcc ttcgcgagca ggcgcggcgc
     3361 cttcttgaac tggcggttcg cggtgaacgg catccagtac gcgtccatcg agggcggctt
     3421 ggcggcggtc gctgcggccg agctcgcggc gagcgtggcg gggtcgtctt tgtgcatgtt
     3481 gtggctccgg gagggatcgt cgtcgcagtg tggggtatgt cgaaccccat tctgagccaa
     3541 gtgcggaacc cgaccgcaga gcgaggccgc atttctcggg agtgtgaacg gagtcttgcg
     3601 cgtgacgcgt gacaatgggt cgctccatag gcgagggatg aaatggaaag ttcgcgcccg
     3661 gtcgtcgtgc tccagaacct ttcggcagat ggcccgggct atctcggcac gtggctggcg
     3721 gagcagggca tcgcgttcga cgtgttcgac gccgaggccg ggaacccgta tccctggtcg
     3781 atcgcgggtt accgcgcgct cgcgctgctg ggcggtgaga tgagcgcgaa cgacgatctg
     3841 ccgtcgttgc gccaggccga gcggctgatc ctgcaggcga tcgacgccga catcccggtg
     3901 atcggccact gcctcggcgg ccagttgatg gcgcgcgcgc tcggcgcgcg ggtgatcgcg
     3961 tcaccgaagc cggagatcgg ctggcaggcg atgcaggtcg ccgacacgcc ggccgcgcgc
     4021 cgctggttcg gcgaacccgg cgagcgcatc gtctaccact ggcacggcga gagcttcgag
     4081 ctgcccgcgg gcgccgagct gctcgccggc aacgcgtcgt gcccgcacca ggccttcgcg
     4141 atcgggcggc acttggcgct gcagttccat gtcgagctcg atgcctgcaa gctcgcggcg
     4201 tggtcggcct catgcgaccc gagcttcgtc cgcctgcagc gcgagtaccc ggcgacggtg
     4261 cagacgggcg ccgcgatgcg cgcgcaggcg ggcaccgcgc tgcccgcaca actgcggctc
     4321 gccgatcgga tgtacgggcg ctggctcggt atcgcgtcgc ccgcgcgctt tcacgatcgc
     4381 gggccgtgat tgcacatcgc ggaacgggcc gatgctgcag tgccgcaatc gctttcgcca
     4441 gcaagaagat gacaccgcat tgcggaatat cagctctaag ctattgattt aaatgattca
     4501 taaatttagt cttatataag atacaagtct tcctcatcgg cgcgtgactc gctattcttg
     4561 agcccagcaa cccgcttctt catcacccac ctcactggag ctcaccatga ccaacctgac
     4621 ccgcgaacaa cagattgccg ccctcgagaa ggactgggcc gagaacccgc gctggaaggg
     4681 catcaagcgc ggctactccg cggccgacgt ggtccgcctg cgcggctcga tcgcgatcga
     4741 gcacacgctc gcccgacgcg gcgccgagaa gctctggaat ctggtcaaca ccgagccctt
     4801 cgtcaacacg ctgggcgcgc tcaccggcaa ccaggcgatg cagcaggtca aggccggcct
     4861 gaaggcgatc tacctgtcgg gctggcaggt ggcgggtgac gccaacctgg ccggcgagat
     4921 gtacccggac cagtcgctgt acccggcgaa ctcggtgccg atggtcgtca agcgcatcaa
     4981 caacaccttc acccgcgccg accagatcca gtggtccgag ggcaagaacg acgtcgattt
     5041 cttcgccccg atcgtggcgg acgcggaagc cggcttcggc ggtgtgctca atgcattcga
     5101 gctgatgaag tcgatgatcg aagcgggcgc ggccggcgtg cacttcgaag accagctcgc
     5161 ctcggtcaag aagtgaggcc acatgggcgg caaggtgctc gtgccgacgc gcgaggcggt
     5221 ctccaagctc accgccgcgc gcctcgcggc cgac
//
LOCUS       DASRDG010000156         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_138513, whole genome shotgun sequence.
ACCESSION   DASRDG010000156 DASRDG010000000
VERSION     DASRDG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138513"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFA35_02530"
     CDS             <1..831
                     /locus_tag="VFA35_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZT55072.1"
                     /translation="GADHYCAAPIVHNLLIAAPAELRVGIGQKVRGMVAGAAPPASMI
                     EGMAKLGFDITHVYGLTEVYGPAAVAVKRKGWAAEDLSEQTRLNGRQGVRYALEEGMT
                     VMDAGNMAQGLQETPDDGETMGEIMFRGNIVMKGYLKNPAATEQAFAGGWFHTGDLAV
                     MEPDRYVKIKDRSKDIIISGGENISSLEVEDALYRHPAVLACAVVAKPDDKWGETPVA
                     FVELRADAQVGAAELVAHCKSLLAGYKVPREVRFEEIPKTSTGKIQKFALRERAKEKP
                     "
     gene            828..1625
                     /locus_tag="VFA35_02535"
     CDS             828..1625
                     /locus_tag="VFA35_02535"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZT55073.1"
                     /translation="MSTDALVLRTRDPRGVVTLTLNRPQAFNALSEALLTALQHELNA
                     IAEDELARVVVLAGAGKAFCAGHDLKEMRADPSLGYYQRLFAQCAAMMMSVQRLAVPV
                     IARVQGVATAAGCQLVAMCDLAVAASDARFAASGVNLGLFCSTPGVALSRNLQRKAAF
                     EMLVTGEFISATQALEKGLVNRVAAPDRLDAEVESLVASIIAKPRVAVALGKQLFYRQ
                     LETGIEAAYEDAAQTMACNMMDASALEGVQAFIEKRPPGWAPPAATG"
     gene            complement(1576..2142)
                     /locus_tag="VFA35_02540"
     CDS             complement(1576..2142)
                     /locus_tag="VFA35_02540"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZT55074.1"
                     /translation="MSDPSRFSEAFRLRSGVAALVRAIRPDDRERLHAAFLELDPESV
                     YLRYFSYKADLSEADLDRLCDPDFRERVVLVVTIAGAAGEVIIAAAGYVAYDTPEHER
                     AAEVAFSVEEDFHGQGISGKLLAVLTTIARADGIDRFEAEVLARNAPMLHVFERSGLP
                     MQHEPAEDGVVRVSLSLLAAPSPAAASR"
     gene            complement(2165..3850)
                     /locus_tag="VFA35_02545"
     CDS             complement(2165..3850)
                     /locus_tag="VFA35_02545"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HZT55075.1"
                     /translation="MTSEEILAQYGPRETMEYDVVVVGGGPGGLAAAIRLKQLATAQG
                     KEISVVVLEKGSEPGAHILSGAVMDPKALTELLPNWKELGAPLNQPVTADQVLFLTET
                     GAVSTPKVLIPECFHNEGNYVISLGNVVRWLATQAEGLGVEIFPGFAATEVLYNDNGS
                     VKGVATGNVGVDKNGQLTGEFQLGMELHAKYTVFAEGARGQLGRQLIAQFKLDEGRDP
                     QSYGIGIKELWEIDPAKHQPGLVVHTAGWPMDSMTYGGGFMYHLEDNKVTLGLVVGLD
                     YQNPWLSPFEEMQRWKTHPRIKAFLEGGKRIGYGARAITAGGLLSLPKLVFPGGALIG
                     CEAGTLNAARIKGSHAAIKTGMLAADAAFAAVTAGRQHDELSAYPQAFEASWLKAELD
                     QSRNFKQWFKKGVYVGSFMTGVEQWLLPMMGVKSPPWTIHRTEPDYARLKPAADCPQI
                     AYPKPDGKITFDRLSSVFISNTNHNEHQPAHLTLKDASVPVNINLAKYAGPESRYCPA
                     GVYEFVKNPDNTDRLQINAQNCVHCKTCDIKDPTQNIVWVAPEGGGGPNYAGM"
BASE COUNT          609 a         1340 c         1405 g          672 t
ORIGIN      
        1 ggcgccgacc attactgcgc ggcgccgatc gtgcacaacc tgctgatcgc ggcgcccgcc
       61 gaactgcgcg tcggcatcgg ccagaaggtg cgcggcatgg tggccggcgc ggcaccgcca
      121 gcctcgatga tcgagggcat ggcgaagctg ggcttcgaca tcacgcacgt ctacggcctg
      181 accgaggtgt acggcccggc cgcggtggcg gtcaagcgca agggctgggc cgcggaagac
      241 ctgtcggagc agacccgcct gaacggccgc cagggcgtgc gctacgcgct cgaagaaggc
      301 atgacggtga tggacgccgg caacatggcg cagggcctgc aggagacgcc ggacgacggc
      361 gagaccatgg gcgaaatcat gttccgcggc aacatcgtga tgaagggcta cctgaagaac
      421 ccggccgcga ccgagcaggc cttcgcgggc ggctggttcc acaccggcga cctcgcggtg
      481 atggagcccg accgctacgt caagatcaag gaccgcagca aggacatcat catctccggc
      541 ggcgagaaca tttcgtcatt ggaagtcgag gacgcgctgt accgccaccc cgcggtgctg
      601 gcctgcgcgg tcgtggcaaa gcccgacgac aagtggggcg agacgcccgt cgcgttcgtc
      661 gagttgcgcg cggacgcgca ggtcggcgcc gccgaactgg tggcgcactg caagagtctg
      721 ctcgcgggct acaaggtacc gcgcgaggtg cgtttcgaag agattccgaa gacctcgacc
      781 ggcaagatcc agaagttcgc gctgcgcgag cgggccaagg aaaaaccatg agcaccgatg
      841 ccctcgtgct gcgcacgcgc gacccgcgcg gcgtcgtgac gctcacattg aaccgcccgc
      901 aggccttcaa cgcgctctcc gaggcgctgc tgaccgcgtt gcagcacgag ctcaatgcca
      961 tcgccgaaga cgaactcgcc cgcgtcgtcg tgctcgccgg cgccggcaag gccttctgcg
     1021 ccggccacga cctgaaggag atgcgcgccg acccgtcgct cggctactac cagcgcctgt
     1081 tcgcgcaatg cgcagcgatg atgatgagcg tgcagcgcct ggccgtgccg gtgatcgcgc
     1141 gtgtgcaggg cgtcgcgacg gcggccggct gccagctcgt cgcgatgtgc gatctggcgg
     1201 tggccgcgag cgacgcgcgc ttcgcggcca gcggcgtgaa cctgggcctg ttctgctcga
     1261 cgccgggcgt ggcgctgtcg cgcaacctgc agcgcaaggc ggcgttcgag atgctggtga
     1321 ccggcgaatt catcagcgcc acgcaggcgc tcgagaaagg cctggtgaac cgcgtcgcgg
     1381 cgccggatcg gctcgacgcc gaggtcgagt cgctggtcgc gagcatcatc gcgaagccgc
     1441 gcgtcgcggt cgcgctcggc aagcagctgt tctaccgcca gctcgagacc ggcatcgaag
     1501 ccgcgtacga ggatgcagcg cagaccatgg cctgcaacat gatggacgcc agcgcgctcg
     1561 aaggcgtgca ggccttcatc gagaagcggc cgccgggctg ggcgccgcca gcagcgacag
     1621 gctgacgcgc acgaccccat cctctgcggg ctcgtgctgc atcggcagcc cgctgcgctc
     1681 gaacacgtgc agcatcggtg cgttgcgcgc cagcacctcg gcctcgaagc gatcgatgcc
     1741 gtcggcgcgc gcgattgtcg tcagcaccgc gagcagcttg ccgctgatgc cctgaccatg
     1801 aaagtcttct tcgacgctga acgcgacctc ggcggcgcgc tcgtgctcgg gcgtgtcgta
     1861 ggcgacgtag ccggcggcgg cgatgatgac ctcgcccgcc gcacccgcaa tggtgacaac
     1921 gagcacgacg cgctcgcgaa agtccgggtc gcacagccgg tcgaggtcgg cttcgctcag
     1981 atcggccttg tacgagaagt agcgcaggta gaccgactcg gggtcgagct cgagaaacgc
     2041 cgcatgcaga cgctcacggt cgtcgggacg gatcgcgcgc accagcgcag cgacgccgct
     2101 gcgcagcctg aacgcctcgc tgaaccggct cgggtcgctc atcgatgcgc tctcgtgccg
     2161 gcgcctacat gcccgcgtag ttcggcccgc caccgccttc gggcgcgacc cagacgatgt
     2221 tctgcgtcgg gtccttgatg tcgcaggtct tgcagtgcac gcagttctgc gcgttgatct
     2281 gcagccggtc ggtgttgtcc gggttcttca cgaactcgta gacgccggcc gggcagtagc
     2341 ggctctccgg gccggcgtac ttcgcgaggt tgatgttgac cgggacgctc gcgtccttca
     2401 gcgtcaggtg cgccggctgg tgctcgttgt ggttggtgtt gctgatgaac acgctggata
     2461 ggcgatcgaa cgtgatcttg ccgtcgggct tggggtaggc gatctgcggg caatcggcgg
     2521 ccggcttcag gcgcgcgtag tcgggctcgg tgcggtggat cgtccacggc ggcgacttca
     2581 cgcccatcat cggcagcagc cactgctcga cgccggtcat gaacgagccg acgtacacgc
     2641 ccttcttgaa ccactgcttg aagttgcgcg actgatcgag ctcggccttc agccagctgg
     2701 cctcgaaggc ctgcgggtag gcgctcagct cgtcgtgctg gcggcccgcg gtgaccgcgg
     2761 cgaacgcggc gtcagccgcg agcatgccgg tcttgatcgc cgcgtggctg cccttgatgc
     2821 gcgcggcgtt cagcgtgccg gcctcgcagc cgatcagcgc gccgccgggg aacaccagct
     2881 tgggcaggct cagcaggccg cccgcggtga tcgcgcgcgc gccgtagccg atgcgcttgc
     2941 cgccttcgag gaaggccttg atgcgcgggt gcgtcttcca gcgctgcatt tcctcgaacg
     3001 ggctcagcca cgggttctgg tagtcgaggc cgacgacgag gccgagcgtg accttgttgt
     3061 cttccaggtg gtacatgaag ccgccgccgt aggtcatcga gtccatcggc cagccggcgg
     3121 tgtgcacgac caggccgggc tggtgcttgg ccgggtcgat ctcccacagc tccttgatgc
     3181 cgatcccgta cgactgcggg tcgcggcctt cgtcgagctt gaactgggcg atcagctggc
     3241 ggccgagctg gccgcgtgcg ccttcggcga acacggtgta cttcgcgtgc agctccatgc
     3301 cgagttggaa ctcgccggtc agctggccgt ttttgtcgac gccgacattg ccggtcgcga
     3361 cgcccttgac gctgccgttg tcgttgtaga gcacctcggt cgcggcgaag ccggggaaga
     3421 tctcgacgcc cagcccctcg gcttgagtgg ccagccagcg caccacgttg cccaggctga
     3481 tgacgtagtt gccttcgttg tggaagcact ccgggatcag gaccttcggc gtgctgaccg
     3541 cgccggtctc ggttaggaac agcacctggt cggcggtgac gggctggttc agcggcgcgc
     3601 cgagttcctt ccagttcggc agcagctcgg tcagcgcctt cgggtccatc accgcgccgc
     3661 tgaggatgtg cgcgccgggc tccgagccct tctcgagcac gacgacggag atttccttgc
     3721 cttgcgccgt ggcgagctgc ttcagcctga tcgccgccgc gagcccgccc ggaccgccgc
     3781 cgacgacgac gacgtcgtac tccatggttt cgcgcgggcc gtactgggcg aggatttctt
     3841 cagacgtcat gcggcttcct tgagcttctc tttggcgcgg attctacggg tcgcgtgcta
     3901 tcgtgattca ctcgcgctcg gccaggccgg ggcgttgcat cggaggaggt tctgctcatg
     3961 agctacaaca tcgatctgtc cggccgtgtc gcgctggtga ccggcgcctc cagcggcctg
     4021 ggcacg
//
LOCUS       DASRDG010000157         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_825688, whole genome shotgun sequence.
ACCESSION   DASRDG010000157 DASRDG010000000
VERSION     DASRDG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825688"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFA35_02550"
     CDS             <1..573
                     /locus_tag="VFA35_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55076.1"
                     /translation="AWEWDGSNWHPQFVASPSPRGGPGLAYDSVRREIVLFGGGISGT
                     TFTDTWLYRTPLPASFTPYGAGCPGSAGTPVLAPAPYSLPWLGDSFVTHVASLPQTAG
                     GVFFLLGLGQTPPVSLAPFGLPGCDTWVTLDESVFAPVVAGTATRTLTVPNATALAGL
                     HVFEQGAVIDSAAPGGAALSNAGEIVLGIR"
     gene            670..1311
                     /locus_tag="VFA35_02555"
     CDS             670..1311
                     /locus_tag="VFA35_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZT55077.1"
                     /translation="MVTSAPGSRRERKKANVRQRILDAAVRLFGAHGIDAVTVDAIAE
                     AADVGKGTIYNHYQTKEDIVVAFMVAMEAQVQKRAAVADARAPAATVLTRFLQRQFKP
                     KEPHHRFVRVFLGEMLAHTEQFLPAMVAMQRVADPPLHELFTRLRARGAIRGDVPAPQ
                     FVLALKTLHLGLTALWAVEGPPFRNTYRTLRLLAQLFGDGLQPRREQTRRKRP"
     gene            1308..>2052
                     /locus_tag="VFA35_02560"
     CDS             1308..>2052
                     /locus_tag="VFA35_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55078.1"
                     /translation="MTNASSPALPSSRTLLCCLALGACAASTETGPRGTTPPDPGQPL
                     ARRYHGEQQFAYAMRAVNEDRHQTLRYSAVAAVHVRPDAGACVEEVDWGQLVVNGRAH
                     PLPASGPAVHQELSLAPDYRWSMPGIAHLDPALIGPVLDLMTFYVDLWLAMKTGKLAR
                     VGDRFVVPRSAPNSWADGRRVLLGEDAIDFDVALAAVDAAARTATVIVQHVPPAQPAI
                     RLPAAWMHAPVADTPNNWVEVERLAADRFV"
BASE COUNT          271 a          760 c          746 g          275 t
ORIGIN      
        1 gcctgggaat gggacggcag caattggcac ccgcagttcg tcgcgtctcc atccccgcga
       61 ggcggcccgg ggctggccta cgacagcgtg cggcgcgaga tcgtgctgtt cggcggcggc
      121 atcagcggca cgaccttcac cgacacctgg ctctaccgca cgccgctgcc ggcgtcgttc
      181 acgccgtacg gcgccggctg cccaggcagc gccggcacgc cggtgctggc gcccgcgccg
      241 tacagcctgc cgtggctcgg cgactcgttt gtcacgcatg tcgcgtcgct gccgcagacg
      301 gcgggcggcg tgttcttcct gctcgggctc gggcagacgc cgccggtgag cctcgcgccg
      361 ttcgggctgc cgggctgcga cacctgggtc acgctcgacg agtcggtgtt cgcgccggtc
      421 gtcgcgggca cggcgacgcg cacgctcacg gtgccgaacg cgaccgcgct cgcaggcctg
      481 cacgtgttcg agcagggggc ggtgatcgac tcggcggcgc ccggcggcgc ggcgctcagc
      541 aacgccggcg agatcgtgct cggcatccgc tgaggggcga ggccgaaggc gcctcccctc
      601 gggattgcgc ggcggcacaa aggagcgggc gttgacccgc ggcggagatg accgtagtca
      661 tattcgacca tggtcacctc tgcgcccgga tcgcgccgcg agcgcaagaa ggcgaacgtg
      721 cggcagcgca tcctcgacgc cgcggtgcgg ctgttcggcg cccacggcat cgacgcggtg
      781 accgtcgacg cgatcgccga ggcggccgac gtcggcaagg gcacgatcta caaccactac
      841 cagaccaagg aagacatcgt cgtcgccttc atggtggcga tggaggcgca ggtgcagaag
      901 cgcgccgccg tcgccgacgc gcgcgccccg gcggcgacgg tgctgacccg gttcctgcag
      961 cggcagttca agcccaagga accccatcac cggttcgtcc gcgtgttcct cggcgagatg
     1021 ctcgcgcaca ccgagcagtt cctgccggcg atggtggcga tgcagcgggt cgcggacccg
     1081 ccgctgcacg agctgttcac ccggctgcgc gcgcgcggcg cgatccgcgg cgacgtgccg
     1141 gcgccgcagt tcgtgctggc gctgaagacg ctgcacctcg gcctcacggc gctgtgggcg
     1201 gtcgaaggcc cgccgttccg caacacgtac cgcacgctgc gcctgctggc gcagctgttc
     1261 ggcgacggac tgcaacccag acgcgaacaa acccgacgca aacgcccatg acgaacgcct
     1321 cgtcgcccgc gctcccgtcg tcccggacgc tgctctgctg cctcgcgctc ggcgcctgcg
     1381 cggcgtccac cgagaccggg ccgcgcggga ccacgccgcc cgatcccggg cagccgctcg
     1441 cgcgccgcta ccacggcgag cagcagttcg cctacgcgat gcgcgcggtc aacgaggacc
     1501 gccaccagac gctgcgctac tcggcggtcg ccgcggtgca cgtgcggccg gacgccggcg
     1561 cctgcgtcga ggaggtcgac tgggggcagc tggtggtgaa cggccgcgcg cacccgctgc
     1621 cggcgagcgg cccggccgtc caccaggaac tgtcgctcgc gcccgactac cggtggtcga
     1681 tgccgggcat cgcgcacctc gacccggcgc tgatcggccc ggtgctcgac ctgatgacgt
     1741 tctacgtcga cctgtggctg gcgatgaaga cggggaagct cgcgcgcgtc ggcgaccgtt
     1801 tcgtcgtgcc gcgcagcgcg ccgaactcgt gggccgacgg ccgccgcgtg ctgctcggcg
     1861 aggatgcgat cgacttcgac gtcgcgctcg ctgccgtcga cgccgccgcg cgcaccgcca
     1921 ccgtgatcgt gcagcacgtg ccgcccgcgc agccggcgat ccggctgccg gcggcgtgga
     1981 tgcacgcgcc ggtcgcggac acgcccaaca actgggtgga ggtggagcgg ctcgcggccg
     2041 accggttcgt gt
//
LOCUS       DASRDG010000158         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_27059, whole genome shotgun sequence.
ACCESSION   DASRDG010000158 DASRDG010000000
VERSION     DASRDG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27059"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFA35_02565"
     CDS             <1..426
                     /locus_tag="VFA35_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HZT55079.1"
                     /translation="RLIDCKRQRAPAEVARALHLGASDPVIRVRRVLSFRDTPVVLDD
                     IWLPSALFKRLTVERLAAHRGPMYGLFETEFGVRMIRAEEKIRAVAADAASAELLALS
                     VGAPLLSVERLALTYGDKPVELRRGLYSTASHYYRNELS"
     gene            569..958
                     /gene="sdhC"
                     /locus_tag="VFA35_02570"
     CDS             569..958
                     /gene="sdhC"
                     /locus_tag="VFA35_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZT55080.1"
                     /translation="MKLIDALGYRLPVAGLVSILHRLSGLVMFVLLPFIIWMFDTSLT
                     SEITYGQFTSVFTAGTGLLPGWFYKLVALVLIWAYLHHLIAGLRHLWMDVSHAAVSKE
                     FGRASAILTLVVSITLTVALGAKLFFF"
     gene            970..1332
                     /gene="sdhD"
                     /locus_tag="VFA35_02575"
     CDS             970..1332
                     /gene="sdhD"
                     /locus_tag="VFA35_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HZT55081.1"
                     /translation="MAQSYGSKRTVVGAHYGLRDWLSQRVTATLMALFTLAVVVQVLL
                     PGEMGYDKWAGIFSKQWMRVLTFVVIVALLVHVWVGVRDVLMDYVKSVGARLALQVAT
                     IVWLVGCAGWAIQVLWRL"
     gene            1353..>2287
                     /locus_tag="VFA35_02580"
     CDS             1353..>2287
                     /locus_tag="VFA35_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT55082.1"
                     /translation="MTAGTASLSKRKFDVVIVGAGGSGMRASLQLSLAGLNVAVLSKV
                     FPTRSHTVAAQGGIGASLGNMSEDNWHYHFYDTVKGSDWLGDQDAIEFMCREAPKVVY
                     ELEHFGMPFDRNPDGTIYQRPFGGHTANYGEKPVQRACAAADRTGHAMLHTLYQQNVK
                     ARTNFFVEWMALDLIRDADGDVVGVTALEMETGEVFILEAKTVLLATGGAGRIFQAST
                     NAFINTGDGLGMAARAGLPLEDMEFWQFHPTGVAGAGVLLTEGCRGEGAILLNNQGER
                     FMERYAPTLKDLAPRDFVSRSMDQEIKEGRGCGPN"
BASE COUNT          365 a          764 c          763 g          395 t
ORIGIN      
        1 cggctgatcg actgcaagcg ccaacgcgcg cccgccgagg tcgcgcgggc gctgcatctg
       61 ggcgcgagcg acccggtgat ccgggtgcgg cgcgtgctgt cgtttcgcga cacgccggtc
      121 gtgctcgacg acatctggct gccctcggcg ctgttcaagc ggctgacggt cgagcggctg
      181 gcggcgcatc gcgggccgat gtatggcctg ttcgagaccg agttcggcgt gcgcatgatc
      241 cgcgccgaag agaagattcg cgcggttgcg gccgatgcgg ccagcgccga gctgctcgcg
      301 ctgagcgtcg gcgcgccgct gctcagcgtc gagcggctcg cgctgaccta cggcgacaag
      361 ccggtcgagc tgcggcgcgg cctgtacagc acggcctcgc actactatcg caatgaactc
      421 agttgatgct cggcctcgat ttcggatgcc gcacgcgcga aatcgtgctg cattgcaata
      481 agatcatcgg ctgcgcagcg gttgctacaa gtccgaacaa ggtttcctca tgacagagat
      541 ggccacaaag caaagacccg gcgggaacat gaaactgatc gacgcgctcg gctaccggct
      601 tccggtggcg ggcctggtgt cgatcctgca ccggctcagc ggcctggtga tgttcgtcct
      661 gctgccgttc atcatctgga tgttcgacac cagcctcacc tccgagatca cctacggcca
      721 gttcacctcg gtgttcaccg caggcaccgg cctcttgccg ggctggttct acaagctcgt
      781 cgcgctggtg ctgatctggg cctacctgca ccacctgatc gccgggctgc gacatctgtg
      841 gatggacgtg tcgcacgccg cggtcagcaa ggagttcggc cgcgcgtcgg cgatcctcac
      901 gctggtcgtc agcatcacgc tcacggtcgc gctcggcgcg aaactgttct tcttctgagg
      961 cgcctgcaca tggcacagag ttacggttcg aagcgcaccg tcgtcggcgc gcactatggt
     1021 ctgcgcgact ggctctcgca gcgcgtcacc gccaccttga tggcgctgtt cacgctcgcg
     1081 gtcgtcgtcc aggtgctgct gccgggcgag atgggctacg acaagtgggc cggcatcttc
     1141 tcgaagcagt ggatgcgggt gctgaccttc gtcgtgatcg tcgcgctgct cgtccacgtc
     1201 tgggtcggcg tgcgcgacgt gctgatggac tacgtgaaat cggtcggcgc gcgcctcgcg
     1261 ctgcaggtgg cgacgatcgt ctggctggtc ggctgcgccg gctgggccat ccaggttctg
     1321 tggagacttt gatgcaagcg ggcattcctc aaatgacggc gggtacggcc tcgctcagca
     1381 agcgcaagtt cgacgtggtg atcgtcggcg ccggcggctc gggcatgcgc gcgtcgctgc
     1441 agctctcgct cgcgggcctg aacgtcgccg tgctgtccaa ggtgttcccg acgcgctcgc
     1501 acacggtcgc tgcacaaggc ggcatcggcg cctcgctggg caacatgagc gaggacaact
     1561 ggcactacca cttctacgac accgtgaagg gctccgactg gctcggcgac caggacgcga
     1621 tcgagttcat gtgccgcgaa gcgccgaaag tcgtctacga gctcgagcac ttcggcatgc
     1681 cgttcgaccg caaccccgac ggcacgatct accagcgccc gttcggcggc cacaccgcga
     1741 actacggcga gaagccggtg cagcgcgcct gcgccgcggc cgaccgcact ggccacgcga
     1801 tgctgcacac gctgtaccag cagaacgtca aggcgcgcac caacttcttc gtcgaatgga
     1861 tggcgctcga cctgatccgc gacgccgacg gcgatgtggt cggcgtgacg gcgctcgaga
     1921 tggaaaccgg cgaggtcttc atcctcgaag ccaagaccgt gctgctcgcc accggcgggg
     1981 ccgggcgcat cttccaggcc tcgaccaacg ccttcatcaa caccggtgac ggcctgggca
     2041 tggcggcgcg tgccggcctg ccgctcgaag acatggagtt ctggcagttc cacccgaccg
     2101 gcgtcgccgg cgccggcgtg ctgctgaccg aaggctgccg cggcgaaggc gccatcctgc
     2161 tcaacaacca gggcgagcgc ttcatggagc gctacgcgcc gacgctgaaa gacctggcgc
     2221 cgcgcgactt cgtctcgcgc tcgatggacc aggaaatcaa ggaaggccgc ggctgcggcc
     2281 cgaacaa
//
LOCUS       DASRDG010000159         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_27079, whole genome shotgun sequence.
ACCESSION   DASRDG010000159 DASRDG010000000
VERSION     DASRDG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27079"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /gene="ilvA"
                     /locus_tag="VFA35_02585"
     CDS             <1..797
                     /gene="ilvA"
                     /locus_tag="VFA35_02585"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HZT55083.1"
                     /translation="ETFRLARELVDEYVVVDTDAACAAIKDVFQDTRSVLEPSGALGV
                     AAIKQYVETQRCKGKTFVAITSGANMNFDRLRFVAERAEVGEEREALFAVTIPEERGS
                     FRRFCELIGPRAVTEFNYRISDAKVAHVFVGLTTTNRAESGKLSKHFKRHGFDTLDLT
                     GDELAKEHVRHMVGGRSSLAQDERLYRFVFPERPGALMRFLSSMHPGWNISLFHYRNQ
                     GADHGRILVGIQVPREDKKGFKEFLEGLAYPCQDETDNPVYRLFLR"
     gene            877..1950
                     /locus_tag="VFA35_02590"
     CDS             877..1950
                     /locus_tag="VFA35_02590"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HZT55084.1"
                     /translation="MSVNRSLIAATSNPLLEQALRQKLARRAETTGGLGELEALAVRL
                     GLIQNTLKPRFRTPQIAIFAADHGLAVEGLGVAGSQSTALLAHQLLTSQLPVAVFARI
                     QGLELSVVDCGVSESVAPHARLLARKIAHGTRNARVTSAMSLDQAHAAIRAGMEIADS
                     LVGNAVVCAGIGVGSHESAALVLSRLSGANLSDLLISGPDMEEADLSHLLAVLQGAQG
                     RHKDVTDPVEVLAAFGGFEMAMMVGVMLVAGSKRHLIVVDGMPACAALIVAARIAPAV
                     TDYCVYCRSHSHEGLDQALALFHASALLELGMESTDGTGATLAWPLVRSAAALLTEVA
                     EGEEPGPTHPGAINTGTEPATPL"
     gene            complement(1951..>2169)
                     /locus_tag="VFA35_02595"
     CDS             complement(1951..>2169)
                     /locus_tag="VFA35_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55085.1"
                     /translation="LELPQPLPAATGSLPASPPGGLPNSPRGGLPASPPTPVPQYVPA
                     PMPGLAPAPNPAGATDAVAPAPTTTQTQ"
BASE COUNT          327 a          768 c          755 g          319 t
ORIGIN      
        1 tcgagacctt ccgcctggcg cgcgagctcg tcgacgagta cgtggtcgtc gacaccgacg
       61 cggcctgcgc cgcgatcaag gacgtgttcc aggacacccg cagcgtgctc gaaccctcgg
      121 gcgcgctcgg cgtcgcggcg atcaagcagt acgtggagac gcagcgctgc aagggcaaga
      181 ccttcgtcgc gatcaccagc ggcgccaaca tgaacttcga ccgcctgcgc ttcgtcgccg
      241 agcgcgccga ggtcggcgag gagcgcgagg cactgttcgc ggtgacgatc cccgaggagc
      301 gcggcagctt caggcgcttt tgcgaattga tcggcccgcg cgcagtgacc gagttcaact
      361 accgcatctc ggatgcaaag gtcgcgcacg tgttcgtcgg cctgacgacg acgaaccgtg
      421 ccgagtccgg caagctcagc aagcacttca agcgccacgg tttcgacacg ctcgacctga
      481 ccggcgacga gctcgcgaag gagcacgtgc gccacatggt cggcggccgc agcagcctcg
      541 cgcaggacga gcggctgtac cgcttcgtct tccccgaacg ccccggcgcg ctgatgcgct
      601 tcctgtcgag catgcacccg ggctggaaca tcagcctgtt ccactaccgc aaccagggcg
      661 cggaccacgg ccgcatcttg gtcggcatcc aggtgccgcg cgaggacaag aagggtttca
      721 aggaattcct cgagggcctg gcctaccctt gccaggacga aaccgacaac ccggtgtacc
      781 ggctgttctt gcgctgatcg tgcattgctc gcgcgccgat tgcgtcctac ggcgccgcgg
      841 ccccacaatt cgcgcgttgc gaatccacga acccccatgt cggtcaatcg ctcgctcatc
      901 gccgccacct cgaacccgct gctcgaacag gccctgcgcc agaagctcgc ccggcgcgcc
      961 gagaccaccg gcggcctcgg cgagctcgaa gcgctcgcgg tgcgcctcgg gttgatccag
     1021 aacacgctga agccgcgctt tcgcacgccg cagatcgcga tcttcgcggc cgaccacggc
     1081 ctcgcggtcg agggcttggg cgtcgccggc agccagtcga cggcgctgct cgcgcaccag
     1141 ttgctgacct cgcagctccc ggtcgccgta ttcgcgcgca tccaggggct ggagctgtcg
     1201 gtggtcgatt gcggtgtatc cgaatcggtc gcgccgcacg cgcggctgct ggcgcgcaag
     1261 atcgcgcacg gcacgcgcaa cgcacgcgtc acttcggcga tgtcgctcga ccaggcacac
     1321 gccgcgatcc gcgccggcat ggagatcgcc gattcgctgg tcggcaacgc ggtggtctgc
     1381 gccggcatcg gcgtcggctc gcatgagagc gcagcgctgg tgctgtcgcg cctgtcgggc
     1441 gcaaacctgt cggacctgct catctccggc ccggacatgg aagaagcgga cctgtcgcac
     1501 ctgcttgccg tgctgcaggg cgcgcagggc cgccacaagg acgtgaccga cccggtcgag
     1561 gtgctggccg cgttcggcgg cttcgagatg gcgatgatgg tcggggtgat gctggttgcc
     1621 ggcagcaagc ggcatctgat cgtcgtcgac ggcatgccgg cgtgcgccgc gctcatcgtc
     1681 gccgcgcgca tcgcgccggc cgtgaccgac tactgcgtgt attgccgcag ccacagccac
     1741 gaggggctgg accaggcgct cgcgctgttc cacgcctcgg cgctgctcga actcggcatg
     1801 gaaagcaccg acggcaccgg cgccacgctg gcctggccgc tggtgcgaag cgccgcggcg
     1861 ctgctgaccg aagtggccga aggcgaggag cccggcccga cacacccggg cgcgatcaac
     1921 accggcaccg agccggcgac gccgctctga ttactgggtc tgggtcgtgg tcggcgccgg
     1981 tgcgacagcg tcggtggcgc cggccgggtt cggcgccggt gcgagccccg gcatcggcgc
     2041 cggcacgtat tgcggcaccg gcgtcggcgg cgaagccggc aagccgcccc gcggcgaatt
     2101 cgggaggccg cccggcggtg aggccggcag gctgccggtg gccgcgggca gcggctgcgg
     2161 tagctccag
//
LOCUS       DASRDG010000160         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1055508, whole genome shotgun sequence.
ACCESSION   DASRDG010000160 DASRDG010000000
VERSION     DASRDG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055508"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VFA35_02600"
     CDS             complement(<1..1349)
                     /locus_tag="VFA35_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HZT55086.1"
                     /translation="MDKILLQIAAELKVRPAQVNAAVELLDGGATVPFIARYRKEATD
                     NLDDIQLRELEARLSYLRELEERRGAVLKSIEEQGKLTPALRAAIAAAPTKQELEDLY
                     LPFKQKRRTKAMIAREAGIEPLADKLFADPRLVPLDEAAAFVNAEAGFADAHAVLDGV
                     RDILSERWAEDAALVRRLRDWLWDEALFQSKLMDGKDGTQPDAAKFRDYFDYAEPIRS
                     VPSHRALAAFRGRALELLDAKLVTDEEIVPGKPVLAEGHIAVHLKWSHAKRPADELIR
                     KCIAWTWKVKLSLSLERDLFARLREEAESVAIRVFAENLRDLLLAAPAGPRVVMGLDP
                     GIRTGCKVAVVDATGKVLATHTVYPLEPRKDWEGSLHTLGLLCATHGVNLIAIGNGTG
                     SRETDKLAADLIKRIQQLAPDTKLDKVVVSEAGASVYSASEFASKELPELDVSLRGAV
                     "
     gene            complement(1447..1878)
                     /locus_tag="VFA35_02605"
     CDS             complement(1447..1878)
                     /locus_tag="VFA35_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55087.1"
                     /translation="MRRFTTSLSVRALAVTAFTLVGACALAATPSRDVQTRYEHERAR
                     CLSGQSGQVQDACLKEAVNARDAALKGQLADGNGRLRQNATQRCDVLAGDDKRDCLAR
                     ITGAANTSESGSVKGGGILRESVTITVEPTPAAPPPPSPMQ"
     gene            complement(2013..>2281)
                     /locus_tag="VFA35_02610"
     CDS             complement(2013..>2281)
                     /locus_tag="VFA35_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZT55088.1"
                     /translation="HQPQRLAEAIKALPLRVRAARPVDEAISTAGGVRFEALDANLMI
                     AALPGVFCAGEMLDWEAPTGGYLLTAAMASGVAAAHAVLRRWPG"
BASE COUNT          307 a          813 c          805 g          356 t
ORIGIN      
        1 acagcaccgc gcaggctcac gtcgagctcg ggcagctcct tgctcgcgaa ctccgaggcc
       61 gaatagacgg atgcgccggc ttcgctgacg acgaccttgt cgagcttcgt gtcgggcgcc
      121 aattgctgga tgcgcttgat cagatcggcg gcgagcttgt cggtctcgcg gctgccggtg
      181 ccgttgccga tcgcgatcag gttcacgccg tgcgtcgcgc acagcaggcc cagcgtgtgc
      241 agcgagccct cccagtcctt gcgcggctcc agcggataga cggtgtgcgt ggccagcacc
      301 ttgccggtcg cgtcgacgac ggccaccttg cagccggtgc ggatgcccgg gtccagcccc
      361 atcacgacgc gcgggccggc cggcgcggcc agcagcaggt cgcgcaggtt ctcggcgaag
      421 accctgatcg cgacgctttc cgcctcttcg cgcaggcgcg cgaacaggtc gcgctccagg
      481 ctcaggctga gcttgacctt ccaggtccag gcgatgcact tgcggatcag ctcgtcggcc
      541 gggcgcttcg catggctcca cttcaggtgc accgcgatat ggccttcggc gagcaccggc
      601 ttgccgggca cgatctcttc atcggtgacg agcttcgcgt cgagcagctc cagcgcgcgg
      661 ccgcggaacg ccgccagcgc gcggtgcgac ggcacgctgc ggatcggctc ggcgtagtcg
      721 aagtagtcgc ggaacttggc ggcgtcgggc tgcgtgccgt ccttgccgtc catcagcttg
      781 ctctggaaca gcgcctcgtc ccacaaccag tcgcgcagcc ggcgcaccag cgcagcgtct
      841 tcggcccagc gctccgagag gatgtcgcgc acgccgtcga gcaccgcatg ggcatcggcg
      901 aaaccggctt cggcgttgac aaacgcggcg gcctcgtcga gcggcaccag cctcgggtcg
      961 gcgaacagct tgtcggccag cggctcgatg ccggcctcgc gggcgatcat cgccttggtg
     1021 cggcgcttct gcttgaacgg caggtacagg tcctcgagct cctgcttggt cggcgccgcg
     1081 gcgatcgcgg cgcgcagcgc cggcgtcagc ttgccctgct cctcgatgct cttcagcacc
     1141 gcgccgcggc gctcttcgag ttcgcgcagg tacgacaggc gcgcttcgag ttcacggagc
     1201 tggatgtcgt cgaggttgtc ggtcgcttcc ttgcggtagc gggcgatgaa cggcaccgtg
     1261 gcgccgccgt cgagcaactc gacggcggca ttgacctggg ccggccgaac cttcagttcg
     1321 gcggcgattt gcagaagaat cttgtccaag ggagaaggcc gcgcccacag ggccgcggcg
     1381 ggcagcgaat cgaagcggcg cagtgtgcca caggcacccg gccgcgccgt gcactgccgc
     1441 gggcggtcac tgcatcggcg acggtggcgg tggagcggcc ggcgtcggct cgaccgtgat
     1501 cgtcacgctc tcgcgcagga tgccgccgcc cttcacgctg ccgctctcgc tggtgttggc
     1561 ggcgccggtg atgcgcgcca ggcagtcgcg cttgtcgtcg ccggcgagca cgtcgcagcg
     1621 ttgggttgcg ttctggcgca gcctgccgtt gccgtcggcc aactggccct tcagcgccgc
     1681 gtcgcgcgcg ttcacggctt ccttcaggca ggcgtcctgc acctggcccg attgcccgct
     1741 caggcagcgc gcgcgctcat gctcgtagcg cgtctgcacg tcgcgcgacg gcgtggcggc
     1801 cagggcacac gcgcccacga gcgtgaacgc cgtgacggca agggcgcgaa ccgacaggga
     1861 ggtggtaaag cggcgcatgg tcggctcctt tgattgatcg atgccaccac tgtagggaca
     1921 gcggcgcgcg ccgcgcgtcg gacaagcgcg cccgcccgcc ttgggcgtgc ccgcccgcgc
     1981 gcgtaggaca tcgccgacgc ggcaggccgc gctcagccgg gccagcggcg cagcaccgcg
     2041 tgcgccgcgg caacgccgct ggccatcgcc gcggtcagca agtagccgcc ggtgggcgcc
     2101 tcccagtcga gcatctcgcc ggcgcagaac acgcccggca acgccgcaat catcaggttt
     2161 gcgtcgagcg cctcgaagcg cacaccgcct gcggtgctga tcgcttcatc gaccggccgc
     2221 gcggcccgca cgcgcaacgg caacgccttg atggcctcgg ccaggcgctg cggctggtgc
     2281 a
//
LOCUS       DASRDG010000161         4357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1287109, whole genome shotgun sequence.
ACCESSION   DASRDG010000161 DASRDG010000000
VERSION     DASRDG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287109"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1602)
                     /locus_tag="VFA35_02615"
     CDS             complement(124..1602)
                     /locus_tag="VFA35_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55089.1"
                     /translation="MPVAKPRPKTRLAATPRWLWWALAACLLAGLWRLGALIVHDPLI
                     ALANSYDEVRYSACFGLYPDRPESVPPDRNSPAAPYSRYKFVAAKDPICYWSTELLFQ
                     GAAAGVFHAEEAATGAMAHSVRWIGAFKIAAMLALWFAFSVAWLRRREPWSALANGLL
                     LPLLFADPANTIYLNTFYAEWTALLALYAVAGLILIHEGRPARGHAFALLALAAAALA
                     VSKIQHIVLPFGIAVAMLALGRWRDRIWLWKGKALLIGALAGIIGQVVQLQRDSEAIR
                     AINVFNQADVVFTALLPNSSDPAATAQFLGLSPQCLQYSGKRAWQMPGFPADLCPELA
                     KVSRSRELVALLREPGMTLRIGWAGLANLHPWVAPGLGLVEGGDFSPLPAQFFSWSDL
                     FAERPVLRTVLFGAPFFAFVALLWRQRWRAWPRLLTVTVLTIVVTLGTLTVTVLGDGL
                     ADVPKQGHLVYNAALVWWIGALVVGWRSLFPATRRRRVPQAS"
     gene            1795..3849
                     /locus_tag="VFA35_02620"
     CDS             1795..3849
                     /locus_tag="VFA35_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZT55090.1"
                     /translation="MKQQLGHYDLVAELGRGGMGVVYKGYEASLNRYVAIKVLADSLA
                     HDPGIKERFLREARSMAALNDPHIIQIYFIGEDEGQTFFVMEFVDGESLSSVLKREHQ
                     LKPDQAAKVIYQTALGLATAHDRGVIHRDIKPGNIMIDSRGNVKIADFGIALSSADIS
                     KKLTSTGEFVGTPGYLSPEVCLGKIVDQRSDIFSLGIVLFEMLAGRMPFTDESPLGLM
                     LEVVKAEIPDVRALNADVDPELSRILSRMIAKEPADRYQSCQDLVADLARNPLLANGP
                     TLQLRTQPSPAAATMVGMKTPASGQSALPNTTPPPRPLPNVTPAPASVATPPPKQTTL
                     PAVTLPDGAHQSLLERQAAASKKKSASPALPLAITALLLLGLGGGAYAYRDQFGFVKQ
                     WLPAGLAGSTSSAATAPVTTATAPGTEPAATPPTASAAATVAATAAPIDTQPAAAPMT
                     TSTTAASANVENASKDANAANSTSTAAAASGASLAELTGGNAASHAAAATAGAATTSA
                     TSSAADNVAATAPVEPPKPLAPPPAAQIAKATPPPPPVAPPKPHVPTIGITFSGDRRA
                     AEPAARMLKAQLTQQGYVVEMVASRASARGRVDALVSVAAEPTGQMDVSAYGQANTAT
                     TAVLNVNVYEMKSGRELSGSSESIVYTGLAAKGQAEEAVQEMAPKIESQLRQYRPGSS
                     RY"
     gene            complement(3896..>4357)
                     /locus_tag="VFA35_02625"
     CDS             complement(3896..>4357)
                     /locus_tag="VFA35_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZT55091.1"
                     /translation="WLWLRAASAIPGVLPPVFHRGEVYVDGAVINNLPVDPMRAMQVG
                     DIIGIDIGADDVLRAQVEEFALPSALRLAWQRLRGHRVRPGILNILLRAGMVNSEAAS
                     VARRAQTSLLLAPPMHDIGLLDWDEYERAIAAGYRYAREVLGRGAKPRSLP"
BASE COUNT          783 a         1516 c         1457 g          601 t
ORIGIN      
        1 ccgcagcgaa gacgccgaac ccacgcgcgc cctgttgcgc gaaaaattgc cggcgcgggt
       61 ttccgcgctg ctgctgcagg gcgatatctg ccgcagcgag ttgctggttg agatcgacgg
      121 cgttcaggac gcctgaggaa cccgacgtcg ccgcgtcgcc ggaaacagcg agcgccagcc
      181 gacgacgagc gcgccgatcc accacaccag cgccgcgttg tagacgagat ggccctgctt
      241 gggcacgtcg gcgaggccgt cgccgagcac ggtgacggtg agcgtgccga gggtgacgac
      301 gatggtcagc acggtcacgg tgagcaggcg cggccaggca cgccagcgct ggcgccagag
      361 cagggcgacg aaggcgaaaa acggcgcgcc gaacaggacc gtccgcaaca cggggcgttc
      421 tgcgaacaga tcgctccagg agaagaactg cgccggcagc ggcgaaaaat caccgccctc
      481 gacgaggccg agtcccggcg ccacccacgg atgcaggttc gcaaggcccg cccagccgat
      541 gcgcagcgtc atgccgggtt cgcgcagcag cgccacgagc tcgcgcgagc gcgacacttt
      601 cgccagctcc gggcacagat cggccggaaa gccgggcatc tgccaggcgc gcttgcccga
      661 gtactgcagg cactgcggcg acaggccgag gaattgcgcg gtcgcggccg gatcgctcga
      721 attcggcagc agcgcggtga acacgacgtc ggcctggttg aacacgttga tcgcgcggat
      781 cgcctcgctg tcgcgctgca gttgcacgac ctggcctatg atgccggcca gtgcgccgat
      841 cagcagcgcc ttgcctttcc acagccagat gcggtcgcgc cagcggccga gcgcgagcat
      901 ggccacggcg atgccgaacg gcagcacgat gtgctggatc ttgctcaccg caagcgctgc
      961 ggcggccagc gcaagcaggg cgaacgcgtg gccgcgcgcg ggcctgcctt catggatcag
     1021 gatcagcccg gcgacggcgt agagcgcgag cagggcggtc cactcggcat agaaggtgtt
     1081 gagataaatc gtgttggccg gatcggcgaa cagcagcggc agcagcagtc cgttggccag
     1141 cgccgaccac ggttcgcgcc ggcgcagcca ggccacgctg aaggcgaacc acagcgcgag
     1201 catcgcggcg atcttgaacg cgccgatcca gcgcaccgaa tgcgccatcg cgccggtcgc
     1261 ggcttcctcg gcgtggaata cgccagccgc cgcgccctgg aacagcagtt cggtcgacca
     1321 gtagcagatc gggtctttcg cggcgacgaa tttgtaccgg gaatacggtg ccgcggggct
     1381 gttgcgatcg ggcggcaccg attcgggccg gtcggggtag aggccgaagc aggcgctgta
     1441 gcgcacctcg tcgtagctgt tggccagtgc gatcaacggg tcgtgcacga tcagtgcacc
     1501 gaggcgccag agcccggcga gcaggcaggc cgcgagcgcc caccacagcc agcgcggcgt
     1561 ggcggcgaga cgcgttttcg ggcggggctt ggcgacaggc ataagggtcg caagtctagg
     1621 gttgctttgt gacgggaaca atcattccgg ggactcacgc aaaaatgtga tccaatgcgc
     1681 agatgcggca tcgattctgc acggtcgttc ccgccaatcg caacgcggac gcgtggaatg
     1741 tgcagtgcta gactttttcg cacttgaacg ggcgccaggc gccttggagg ggtcatgaag
     1801 cagcaactgg ggcattacga tctggtcgcc gaactcggcc gtggcggcat gggtgtggtc
     1861 tacaaaggct acgaggcgtc gctgaaccgt tatgtcgcga tcaaggtcct cgccgactcg
     1921 cttgcccacg atccggggat caaggagcgt ttcctgcgcg aggcgcgcag catggccgca
     1981 ctcaacgacc cgcacatcat ccagatctac ttcatcggcg aggacgaggg gcagaccttc
     2041 ttcgtcatgg aattcgtcga cggcgaatcc ttgagttccg tactcaagcg cgaacaccag
     2101 ctcaagccgg accaggccgc gaaggtcatc taccagaccg cactcggcct cgccaccgcg
     2161 cacgaccgcg gcgtgatcca tcgcgacatc aagcccggca acatcatgat cgacagccgc
     2221 ggcaacgtga agatcgccga cttcggcatc gcgctgtcga gcgccgacat ttccaagaag
     2281 ctcacctcga ccggcgagtt cgtcggcacg ccgggctatc tctcgcccga ggtctgcctc
     2341 ggcaagatcg tcgaccagcg ttcggatatt ttttccctcg gtatcgtgct gttcgagatg
     2401 ctcgccgggc gcatgccgtt caccgacgag tcgccgctcg gtctcatgct cgaagtggtc
     2461 aaggccgaga ttcccgacgt gcgcgcgctc aacgccgacg tcgatccgga actctcgcgc
     2521 atcctttcgc gcatgatcgc caaggaaccg gccgaccgtt accagagctg ccaggacctc
     2581 gtcgccgacc ttgcccgcaa cccgctgctc gccaacggcc cgacgctgca gctgcgcacc
     2641 cagccctcgc cggccgcggc gaccatggtc ggcatgaaga cgccggcgtc gggtcagagc
     2701 gcgctgccga acacgacgcc accgccgcgc ccgctgccga acgtgacgcc cgcgccggcc
     2761 agcgtggcca cgccgccgcc caagcaaacc acgctgccgg cggtgacgtt gccggacggc
     2821 gcgcaccagt cgctgctcga acgccaggcc gccgcatcga agaagaaatc cgcctcgccc
     2881 gcgctgccgc tggcgatcac cgcgctgctg ttgcttggcc tcggcggcgg cgcgtatgcc
     2941 taccgcgacc aattcggctt cgtgaagcaa tggctgccgg caggcctggc cggctcgacg
     3001 tcgtcggccg cgacggcgcc agtcacgacg gcaaccgcac cgggcacgga gcctgccgca
     3061 actccgccaa ccgcatctgc ggcggcgacg gtcgccgcaa cggcggcgcc gatcgacacg
     3121 cagccggcgg cagcgccgat gacaacctcc accaccgccg catccgcaaa tgtggaaaat
     3181 gcatcaaaag atgcaaatgc tgcaaattcc acttctaccg cggcagctgc cagcggcgcc
     3241 tctctggccg agctgaccgg cggcaacgcg gcatcccacg ccgccgcggc gacggctggt
     3301 gctgccacga cttcagccac gtccagcgcg gccgacaatg tcgccgcgac cgctccggtc
     3361 gaaccgccga aaccgctcgc gccgccgccc gccgcgcaga tcgccaaggc aacgccgccg
     3421 ccaccgccgg tcgcgccgcc gaagccgcac gtgccgacga taggcatcac gttcagcggc
     3481 gatcggcgcg ccgcggagcc cgccgcgcgc atgctcaagg cgcaattgac gcagcaaggc
     3541 tatgtcgtcg aaatggtcgc ttcgcgcgcc agcgcgcgcg gccgtgtcga cgcactcgtc
     3601 agcgtcgccg ccgagccgac cgggcagatg gacgtctcgg cctatgggca ggccaacacg
     3661 gcaacgaccg ccgtgctcaa cgtgaacgtg tacgagatga aaagcggccg cgagctgagc
     3721 ggctccagcg aatcgatcgt ctacaccggc cttgccgcga agggacaggc cgaggaagcg
     3781 gtgcaggaga tggcgccgaa gatcgagtcg cagctgcgcc agtaccggcc cggttccagc
     3841 cggtactgat ccgtcgacct gcagggcgcc gggcaacggg cgcccggcga aggtttcacg
     3901 gcaggctgcg cggcttggcg ccgcgcccga gcacctcgcg tgcgtagcgg tagcctgcgg
     3961 cgattgcgcg ctcgtattcg tcccagtcga gcaggccgat gtcgtgcatc ggcggcgcga
     4021 gcagcaggct cgtctgcgcg cgtcgggcca cgctcgcggc ttccgaattg accatgcccg
     4081 cgcgcagcag gatgttgagg ataccgggcc gcacacggtg cccgcgcaga cgctgccagg
     4141 cgagccgcaa cgccgaaggc aaggcgaatt cctcgacctg cgcgcgcagc acgtcgtcgg
     4201 cgccgatatc gatgccgatg atgtcgccga cctgcatcgc gcgcatcgga tcgaccggca
     4261 ggttgttgat caccgcaccg tcgacgtaga cctcgccgcg atggaacacc ggcggcagca
     4321 cgcccggaat cgcgctcgcc gcgcgcagcc acaacca
//
LOCUS       DASRDG010000162         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_826610, whole genome shotgun sequence.
ACCESSION   DASRDG010000162 DASRDG010000000
VERSION     DASRDG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826610"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="VFA35_02630"
     CDS             complement(<1..1676)
                     /locus_tag="VFA35_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZT55092.1"
                     /translation="MIAQELEVSLHMAFVEARQQRHEFITVEHLLMALLDNPSAAEVL
                     RACSANADDLRKSLVGFIKENTPTVGGTDEVDTQPTLGFQRVIQRAIMHVQSTGSGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNFIAHGIRKSDPPEPAKPSEGSAG
                     GNETEKEEGEGKGSPLDQFTQNLNQLARDGKIDPLIGREHEVERVIQILCRRRKNNPL
                     LVGEAGVGKTAIAEGLAWRITQKEVPEVLADSVVYSLDMGALLAGTKYRGDFEQRLKG
                     VLKQLKDQPNAVLFIDEIHTLIGAGAASGGTLDASNLLKPALSSGAMKCIGATTFTEY
                     RGIFEKDAALSRRFQKVDVVEPSVEQTVEILKGLKSRFEEHHSVKYAVGALQAAAELS
                     AKFINDRHLPDKAIDVIDEAGAAQRILPKSKQKKTITRNEVEEIVAKIARIPPAAVSN
                     DDRSKLKTLDRDLNSVVFGQEPAVEAISAAIKMARSGLGRPDKPIGSFLFSGPTGVGK
                     TEVAKQLAYILGVELMRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEA"
     gene            complement(1673..2029)
                     /gene="clpS"
                     /locus_tag="VFA35_02635"
     CDS             complement(1673..2029)
                     /gene="clpS"
                     /locus_tag="VFA35_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HZT55093.1"
                     /translation="MPNEPTPPSAPPVAPPKPDDGQGAVVVERKVAKTEPPRMYQVVL
                     LNDDYTPMEFVVMVLQEYFKHDLETATQIMLKIHHDGRGVCGVYSKDVAATKVELVLA
                     AARRGGHPLQCIMEAA"
BASE COUNT          313 a          724 c          716 g          446 t
ORIGIN      
        1 gcctctgtga gcaggcctcc ttggtcgaag ccgacgtagc ccggaggcgc gccgatcagc
       61 cggctcaccg cgtggcgctc catgtactcg ctcatgtcga agcgcatcag ctcgacgccg
      121 aggatgtagg cgagctgctt cgcgacctcg gtcttgccga cgccggtcgg cccgctgaac
      181 aggaacgaac cgatcggctt gtcgggccgg cccaggcctg agcgcgccat cttgatcgcg
      241 gcggagatcg cctcgaccgc cggctcctgg ccgaacacga cgctgttcag gtcgcggtcg
      301 agcgtcttca gcttcgagcg gtcgtcgttc gacaccgcgg cgggcgggat gcgcgcgatc
      361 ttcgcgacga tctcttcgac ctcgttgcgc gtgatcgtct tcttctgctt gctcttgggc
      421 aggatgcgct gcgccgcgcc ggcctcgtcg atcacgtcga tcgccttgtc gggcaagtgg
      481 cggtcgttga tgaacttggc cgatagttcg gccgccgcct gcagcgcgcc gaccgcgtac
      541 ttgacgctgt gatgctcctc gaagcgcgac ttcagaccct tcaggatctc gaccgtctgc
      601 tcgaccgaag gttcgaccac gtcgaccttc tggaagcgcc gcgacagcgc cgcatccttc
      661 tcgaagatgc cgcggtactc ggtgaaggtg gtcgcgccga tgcacttcat cgcgccggag
      721 ctcagcgccg gcttgagcag gttgctcgcg tccagcgtgc cgcccgaggc cgcgccggcg
      781 ccgatcaacg tgtggatctc gtcgatgaac agcaccgcgt tcggctggtc cttgagctgc
      841 ttcaagacgc ccttcaggcg ctgctcgaaa tcgccgcggt acttggtgcc ggccagcagc
      901 gcgcccatgt cgagcgagta gacgaccgaa tcggccagca cttcgggcac ttctttctgg
      961 gtgatgcgcc aggccaggcc ctcggcgatc gcggtcttgc ccacacccgc ttcgccgacc
     1021 agcagcgggt tgttcttgcg ccgccggcac aggatctgga tcacgcgctc gacctcgtgc
     1081 tcgcggccga tcagtgggtc gatcttgccg tcgcgggcga gctggttcag gttctgcgtg
     1141 aactggtcga gcggcgagcc cttgccctcg ccctcttcct tctcggtctc gttgccgccc
     1201 gcgctgcctt cgctcggctt cgccggctcg ggcggatcgc tcttgcggat gccgtgggcg
     1261 atgaagttca ccacatcgag gcgggtcacg ccctgctggt gcaggtagta gacagcgtgt
     1321 gagtccttct cgccgaagat cgcgaccagc acgttcgcgc cggtcacttc cttcttgccg
     1381 ctgccggtcg actgcacgtg catgatcgcg cgctggatca cgcgctggaa gcccagcgtc
     1441 ggttgcgtgt cgacctcgtc ggtgccgccg acggtgggcg tgttttcctt gatgaacccg
     1501 accaggctct tgcgcaggtc gtcggcgttg gccgagcagg cgcgcagcac ttcggcagcg
     1561 gaagggttgt cgagcagcgc catcagcagg tgctcgacgg tgatgaactc gtggcgctgc
     1621 tggcgcgcct cgacgaacgc catgtgcagg ctgacttcaa gttcctgggc aatcatgcgg
     1681 cctccataat gcattgcaac gggtggccac cgcggcgcgc ggcggccagc accaattcaa
     1741 ccttcgtcgc tgcgacgtcc ttcgagtaga cgccgcagac accccgtccg tcatggtgga
     1801 tcttcagcat gatctgggtc gcggtttcga ggtcgtgttt gaagtactcc tgcagcacca
     1861 tcacgacgaa ctccatgggc gtgtagtcgt cgttgagcag gaccacctgg tacatgcggg
     1921 gtggttctgt ctttgcaacc ttgcgctcga caacgacagc cccctgcccg tcgtcgggct
     1981 tcggcggcgc caccggaggc gctgagggtg gggtcggctc gttgggcatg gattcattct
     2041 atcgagttga cgccgcagcg tttcacccga tcccatattg cgcccgcacc gcgtttcgca
     2101 aggtccgaac tgcgtcacgg cagtggtgct cgtcgggcgg tgtttccaat cttgacagtc
     2161 gatttcgggc ggcgggaaaa tgtgacgcgc gtcacgaat
//
LOCUS       DASRDG010000163         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_826873, whole genome shotgun sequence.
ACCESSION   DASRDG010000163 DASRDG010000000
VERSION     DASRDG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826873"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /gene="phnE"
                     /locus_tag="VFA35_02640"
     CDS             <1..787
                     /gene="phnE"
                     /locus_tag="VFA35_02640"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HZT55094.1"
                     /translation="ALGELQARPAAQRWRRAGIALALLAFYVLCWKLADIDLARLASG
                     LPKMGRWLASAWPPAWSELPVFLLRTAETVAMAALGTTAAVLLAVPMAVLAARNVTPW
                     PRAVGPARLLLNALRGIDSFLFALLFVAAVGLGPFAGVLGIALHTWGSAAKNFADHLE
                     NVSLEAFEAVRSTGASRSMALAYAVLPDVLPVLLSTALFWWEFNVRASTVLGVVGAGG
                     IGQELKSSMDLLDFPRLFTIIVVIVVVVTLLDRLSGWLRQRLT"
     gene            784..1530
                     /gene="phnC"
                     /locus_tag="VFA35_02645"
     CDS             784..1530
                     /gene="phnC"
                     /locus_tag="VFA35_02645"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HZT55095.1"
                     /translation="MTAPLLQVRELGHGYGDRPVLDGVNLDVHRGEFVALLGPSGAGK
                     TTLIKCITGLVRPQRGQVLVGGAEPASLHGRERRRVAVIFQQFNLVRRLSALDNVLAG
                     RLGHVPAWRGWTRQFERADQRLALECLERVGLLAAASQRADTLSGGQQQRVAIARALA
                     QQPELIVADEPVASLDPAISAGILELLQGICRDDGVTVVCSLHQLHLARRYAQRMVGL
                     AAGRVRFDARTDGIDAAATAALYDSAFAAA"
     gene            1551..>2704
                     /locus_tag="VFA35_02650"
     CDS             1551..>2704
                     /locus_tag="VFA35_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HZT55096.1"
                     /translation="MQPSATHTLHPDVAFDGGDLDCGNGLLLLIRKHIDPLPRGGLLE
                     IRSTEISVDEDLPAWCRMTKNELVSWTKEGKQRSFLVCKGALEERHAGLPAQPRAAAL
                     VAAPAATAAPHREPVAAPVIPPLAVTGMGSWPRPRWLVDAMHAHVQGRLDDAAFQETA
                     DDAVRLIAAAQQRAGATLFTDGGQRRDSYASFVATRLDNCQLVPLIDLLPLVEDPAAF
                     EAELRALDVPASEVRHPGVFGRIRRSRPIAVHELDFLRTLTPAPIKVALPGPYLLTRT
                     MWMDCIADSVYATREQIAADIVRVLREEVIDLLNAGASLVQLDEPVLSEVVFSGPKNK
                     RSFMCGALSESEGPEHELGFARELVNAVVDGLPRERIALHVCRGNWTPDES"
BASE COUNT          340 a          993 c          974 g          397 t
ORIGIN      
        1 ggcgctgggc gagttgcagg cccggcccgc ggcgcagcgc tggcggcggg ccggtatcgc
       61 cctggcgctg ctggcgttct acgtcctgtg ctggaagctg gccgacatcg acctcgcgcg
      121 cctggccagc ggcctgccga agatggggcg ctggctcgcg tcggcctggc cgccggcctg
      181 gtccgagctg ccggtgttcc tgctgcgcac ggccgagacg gtggcgatgg cggcgctcgg
      241 caccaccgcc gcggtgctgc tggcggtgcc gatggccgtg ctggcggcgc gcaacgtgac
      301 gccctggccg cgtgccgtgg gtccggcgcg gctgctgctc aacgcgctgc gcggcatcga
      361 ttcgttcctg ttcgcgctgc tgttcgtcgc cgcggtgggc ctgggcccgt tcgccggcgt
      421 gctgggcatc gcgctgcaca cctggggcag cgcggcgaag aattttgccg accacctcga
      481 gaacgtctcg ctcgaagcct tcgaggccgt gcgcagcacc ggcgcgtcgc gctcgatggc
      541 gctggcctac gcggtgctgc ccgacgtgct gccggtgctg ctgtcgaccg cgctgttctg
      601 gtgggagttc aacgtgcgcg cgtccaccgt gctcggcgtc gtcggtgccg gcggcatcgg
      661 gcaggagctg aagagcagca tggacctgct cgacttcccg cggctgttca cgatcatcgt
      721 cgtcatcgtc gtcgtcgtga cgctgctcga ccgcctctcg ggctggctgc gccagcggct
      781 gacgtgacgg cgccactgct gcaggtgcgc gaacttggcc atggctacgg cgatcggccg
      841 gtgctggacg gcgtgaacct cgacgtgcac cggggcgagt tcgtcgcgct gctgggcccg
      901 agcggcgcag gcaagaccac gctcatcaag tgcatcaccg ggctggtgcg gccgcagcgc
      961 ggccaggtgc tggtaggcgg ggccgagccg gcgtcgctgc acggccgcga acggcgccgc
     1021 gtggcggtga tcttccagca gttcaacctg gtgcgccgcc tgtccgcgct cgacaacgtg
     1081 ctggccggcc gcctcggcca tgtgcccgcg tggcgcggct ggacgcgcca gttcgagcgc
     1141 gccgaccagc gcctcgcgct ggagtgcctg gaacgcgtgg gcctgctggc cgccgccagc
     1201 cagcgcgccg atacgctgag cggtggccag cagcagcgcg tcgcgattgc ccgcgccctg
     1261 gcgcaacaac ccgagctgat cgtcgccgac gagccggtgg ccagcctcga ccccgccatc
     1321 agcgccggca tcttggagtt gctgcaaggc atctgccgcg acgacggcgt gacggtggtg
     1381 tgcagcctgc atcagctgca cctcgcgcgg cgctacgcgc agcgcatggt cgggctggcg
     1441 gccgggcggg tgcgcttcga cgcgcgcacc gacggcatcg atgccgcggc caccgcggcg
     1501 ctctacgact ccgctttcgc agcggcctga cccccacgag gacctcgacc atgcagccca
     1561 gcgccaccca caccctccac cccgacgtcg cgttcgacgg cggcgatctc gactgcggca
     1621 acggcctgct gctgctgatc cgcaagcaca tcgacccctt gccccgcggc gggctgctcg
     1681 agatccgctc gaccgagatc tcggtcgacg aggacttgcc ggcctggtgc cgcatgacca
     1741 agaacgagct cgtgtcctgg accaaggaag gcaagcagcg cagcttcctg gtctgcaagg
     1801 gtgcgctgga agagcgccat gccggcctgc ccgcacagcc gcgcgctgcg gccctggtcg
     1861 ctgcgcccgc cgcgacggcg gcgccgcacc gcgagcccgt cgcggcaccc gtcatcccgc
     1921 cgctggccgt caccggcatg ggcagctggc cgcgcccgcg ctggctggtc gacgcgatgc
     1981 acgcgcacgt gcaaggccgg ctggacgacg cggccttcca ggagaccgcc gacgacgcgg
     2041 tgcgcctgat cgccgccgcc cagcagcgcg ccggcgccac gctgttcacc gacggcgggc
     2101 agcgccgcga cagctacgcc agcttcgtcg cgacgcgcct ggacaactgc caactcgtgc
     2161 cgctgatcga cctgctgccg ctggtcgaag acccggccgc cttcgaggcc gaactgcgcg
     2221 cgctcgacgt gccggccagc gaggtgcgcc accccggcgt gttcggccgc atccggcgca
     2281 gccggccgat cgcggtgcac gagctcgact tcctgcgcac gctcacgccg gcgccgatca
     2341 aggtggcgct gcccggcccc tacctgctga cgcgcaccat gtggatggac tgcatcgccg
     2401 acagcgtcta cgccacgcgc gagcaaatcg ccgccgacat cgtgcgcgtg ctgcgcgagg
     2461 aggtgatcga cctgctgaac gccggcgcgt cgctggtgca gctcgacgag ccggtgctgt
     2521 ccgaagtcgt gttcagcggc ccgaagaaca agcgcagctt catgtgcggc gcgctgtccg
     2581 aatccgaggg gcccgagcac gagctggggt tcgcgcgcga gctggtcaat gccgtcgtcg
     2641 acggcctgcc gcgagagcgc atcgcgctgc atgtgtgccg cggcaattgg acgcccgacg
     2701 agtc
//
LOCUS       DASRDG010000164         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1176043, whole genome shotgun sequence.
ACCESSION   DASRDG010000164 DASRDG010000000
VERSION     DASRDG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176043"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFA35_02655"
     CDS             complement(<1..582)
                     /locus_tag="VFA35_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZT55097.1"
                     /translation="MTLKTASFTPPLRLTALLAALAAATALSACAPLLLGGAIVGSSM
                     VVTDRRTSGAQVDDQAIELKAVKRINETLGDRGSVSTTSYNRVVLLTGSVTDEADKAA
                     VERAVAGIESVKSVVNEVSIGPAASFGNRSNDTLLTSKVKASLVDAKDVQVNAYKVVT
                     ERGIVYLMGRVTEREANRAADIARGVSGVQKVVK"
     gene            complement(579..1175)
                     /locus_tag="VFA35_02660"
     CDS             complement(579..1175)
                     /locus_tag="VFA35_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HZT55098.1"
                     /translation="MLEQRIQQQFFDSADLKYAAAEVLAKPIADAVSALVGCITAGGK
                     VLVCGNGGSAADAQHFAAEFVGRFERERPGLAAIALTTDSSILTAIANDYDFNAIFSK
                     QVQALGAPGDVLLAISTSGNSSNVIAAIEAARAKEMVVIAMTGHKGGKIRDLLTETDV
                     LICVPHERTARIQEVHLLSLHCLCDAVDLQLLGEQENT"
     gene            complement(1247..1618)
                     /locus_tag="VFA35_02665"
     CDS             complement(1247..1618)
                     /locus_tag="VFA35_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZT55099.1"
                     /translation="MATATKESGDAGEARALAHLLAQGLTLVQRNYRVARGPHARGGE
                     IDLILRERDGTLVFAEVRARRGGTHGGAAASVGAAKQRSLIFAAQHFLRTQRSVPPCR
                     FDVVAIDGDRIEWLRGAFDAS"
     gene            1665..>2109
                     /locus_tag="VFA35_02670"
     CDS             1665..>2109
                     /locus_tag="VFA35_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZT55100.1"
                     /translation="MLQAAASAAGAQHYPPGALYVVATPIGNLADVTLRAIHVLALVD
                     AIACEDTRHSGPLLRQLGIDAKALLAVHEHNEREAAAGVLARLARGERVAYISDAGTP
                     AISDPGAALVAAVQAAGHRALPIPGASSAVAALSVAGDATGGPFTF"
BASE COUNT          276 a          747 c          752 g          334 t
ORIGIN      
        1 cttcacgacc ttctgcacgc cgctgacgcc gcgtgcgatg tcggctgcac ggttcgcctc
       61 gcgctcggtc acgcggccca tcaggtagac gatgccgcgc tcggtgacga ccttgtaggc
      121 gttcacctgc acgtccttgg cgtcgaccag gctggccttg accttgctcg tcagcagcgt
      181 gtcgttcgag cggttgccga acgacgccgc ggggccgatc gagacctcgt tgacgaccga
      241 cttgacgctc tcgatgccgg ccaccgcgcg ctcgaccgcg gctttgtcgg cctcgtcggt
      301 gacgctgccg gtcagcagca cgacgcggtt gtagctggtg gtgctgacgc tgccgcggtc
      361 gccgagcgtc tcgttgatgc gcttgacggc cttcaactcg atcgcctggt cgtcgacctg
      421 cgcgcccgag gtgcggcggt cggtcacgac catcgagctg ccgacgatgg cgccgccgag
      481 cagcagcggg gcgcaggcgg agagtgcggt ggcggcggcg agcgccgcga gcagcgcggt
      541 caggcgcagc ggtggagtga aagaagcggt cttcagcgtc atgtgttttc ctgttcgccc
      601 agcagttgca gatcgaccgc gtcgcacagg cagtgcaggc tcagcagatg cacctcctgg
      661 atgcgcgcgg tgcgctcgtg cgggacgcag atcagaacgt cggtctcggt cagcagatcg
      721 cggatcttgc cgcccttgtg gccggtcatc gcgatcacga ccatctcctt ggcgcgcgcg
      781 gcctcgatcg ccgcgatcac gttgctcgaa ttaccgctgg tgctgatcgc cagcagcacg
      841 tcgccgggtg cgccgagcgc ctgcacctgc ttcgagaaga tcgcgttgaa gtcgtagtcg
      901 ttggcgatcg cggtcaggat cgagctgtcg gtcgtcagcg cgatcgccgc caggcccggg
      961 cgctcgcgct cgaagcggcc gacgaactcg gcggcgaagt gctgcgcgtc ggcggccgag
     1021 ccgccgttgc cgcagaccag caccttgccg cccgcggtga tgcagccgac cagcgcgctg
     1081 accgcgtcgg cgatcggttt ggcgagcact tcggcggcgg cgtactttaa gtcggcgctg
     1141 tcgaagaact gctgttggat tcgttgttca agcatgggca cggatcatat gacagcggtg
     1201 caccaccgcc ggttccctcg gccggccgcc atcgggcgca tttgcgtcag gatgcgtcga
     1261 acgcgccgcg cagccactcg atgcggtcgc cgtcgatcgc gacgacgtcg aaacggcagg
     1321 gcgggacgct gcgctgcgtg cgcaggaagt gttgcgccgc gaagatcagg ctgcgctgtt
     1381 tggccgcgcc gacgctggcc gccgcgcccc cgtgcgtgcc gccgcggcgc gcgcgcacct
     1441 cggcgaacac cagcgtgccg tcgcgctcgc gcaggatcag gtcgatctcg ccgccgcgcg
     1501 cgtgcggccc gcgcgcgacc cgataattgc gctgcaccag ggtcaggccc tgggccagca
     1561 ggtgcgccag ggcgcgcgcc tcgccggcat cgccgctctc ttttgtcgcc gtcgccactg
     1621 tcgtcgttcc cggaaccctc gtgaccccac ccgccgcctc gtcgatgctg caagccgccg
     1681 cgagcgcggc cggcgcgcag cattacccgc ccggcgcact gtacgtggtc gccacgccga
     1741 tcggcaacct ggccgacgtc acgctgcgcg cgatccacgt gctcgcgctg gtcgacgcga
     1801 tcgcctgcga ggacacccgc cacagcggcc cgttgctgcg ccagctcggc atcgacgcca
     1861 aggcgctgct cgcggtgcac gagcacaacg agcgcgaggc ggcggctggc gtgctggcgc
     1921 ggctggcacg cggcgagcgc gtcgcttaca tcagcgacgc cggcacgccg gcgatcagcg
     1981 accctggcgc ggcgctggtc gccgcggtgc aggcggcggg gcaccgcgcg ctgccgatcc
     2041 ccggcgcgag cagcgcggtg gcggcgctca gcgtggccgg cgatgcgacc ggcgggccgt
     2101 tcacgttca
//
LOCUS       DASRDG010000165         4474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1176152, whole genome shotgun sequence.
ACCESSION   DASRDG010000165 DASRDG010000000
VERSION     DASRDG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176152"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFA35_02675"
     CDS             <1..826
                     /locus_tag="VFA35_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT55101.1"
                     /translation="AGGTLDKVKASGTITLAYRESSIPFSYLDDKGQPVGFGHEICLK
                     IVDEVKKATGRPDLKVALQSVTSANRIPLLQNGTIDIECGSTTNNSERGKQVQFATNY
                     FYTGTRFLVKTGTQVKSIDDLKGKTIVSTSGTTNYKILRNLNEEKKLGIDLLAAKDHA
                     ESALMVESGRALAFGMDDILLYGLRASSQNPAGLAVVGEPIQVEPYAIMLRLDDPAFK
                     KLVDATLAGLMKSGEFTKLYTKWFMSPIPPKGINLNAPMSKELVDNMKALSDKPAT"
     gene            876..1118
                     /locus_tag="VFA35_02680"
     CDS             876..1118
                     /locus_tag="VFA35_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55102.1"
                     /translation="MANMTIAVDDQLLRAARIKAVQQGTSVNEICRQAIEAFAQGAGD
                     ADARIARIKALAKKVRAGDEPLWPGRDALYAESPAR"
     gene            1115..1564
                     /locus_tag="VFA35_02685"
     CDS             1115..1564
                     /locus_tag="VFA35_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZT55103.1"
                     /translation="MSCFFDTNVLVYVFDRAEPAKQALAQELVTEHMSARDMVLSTQV
                     LQELYVTLTRKKRLGTADALEVVTTFAQERVVPASADTVLRGLALSQRHQLSAWDALI
                     LQAALDAGCTTLFSEDFQAGARFDDLVVVNPFAPQVQAPRAAYGKRR"
     gene            1649..3271
                     /locus_tag="VFA35_02690"
     CDS             1649..3271
                     /locus_tag="VFA35_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZT55104.1"
                     /translation="MSNPPRLALTAVALTALLAACSKPAEAPPHVLNTPAPPVATPPA
                     DHAESGIAWKHAASDADVDAAFALAKAENKPVFVYWGAKWCPPCNQVKATLFNRQDFI
                     ERSRAFVPVYVDGDSPGAQKLGSRFKVSGYPTMLLFNPQGTEMTRLPGEVEAERYTQV
                     LTLGMNAQRPVSEVLAQAKAGGAGMGANDWRLLAFYSWDTDQQLVPKGGVPALLKQLA
                     DACPADQNETATRLRLKALAAADANAPAKPDPAAQATLLKVLADAPTARAYLDVLTNY
                     ATDITRALSAKGTPQRAELVAAFNAALKRFEADTTLSRADRLGAIAARVDLARMDAPK
                     RAAVQLPDALVADAREHAARADREITDGYERQAVITSAAHLLTDVGLVAESDTMLKNS
                     LAKSHSPYYLMLALASNAKKRGDTADALHWYEESFAKSEGPATRLQWGAGYLNALIDL
                     APHDEARIEKAASQLFAEAAKQPDAFYERSGRSLQRVASKLQGWNKGGAHAAAIKRLQ
                     AQLDGVCAALVADDPKRATCAALLKPTAKTTA"
     gene            3338..>4474
                     /locus_tag="VFA35_02695"
     CDS             3338..>4474
                     /locus_tag="VFA35_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZT55105.1"
                     /translation="MPKPRARTPAPPLPSTAFDRQRRAVLAAAAAALGGSALPRAAAA
                     DDGPVLGVWTHAYAAFGPPKYPRGFAHFEYVNPAAPKGGTLYLRNPDRRSSFDKFNYY
                     TLKGNSPGGLQIFMFEPLAILSGDEPQTMYGLLAEEMLIAPDKASMTIRLHPKARFYN
                     GDPVTAADVKHSFDSLAGKFASPTLQSQMAAVGAAVVLDPRTVRFDLKERNTDTLFLV
                     GGLPVFSAKWGLEPDGKRKPFDQIVTEYPITTGPYTIGLVDSNRRIEFARNPEYWARD
                     LPVRRGFFNFDRVVYRYYKDNDVAREAFRAGEFDILKEYSARSWVRQHAGPKWQSGQI
                     IKDPFPTAFGEMQQAFDLNLRRPKFQDLRVRRAIDLTYDFEMVVN"
BASE COUNT          784 a         1610 c         1479 g          601 t
ORIGIN      
        1 ggccggcggc accctcgaca aggtcaaggc ctcgggcacg atcacgctgg cctaccgcga
       61 atcgtcgatc ccgttctcgt acctcgacga caagggccag ccggtcggct tcggccatga
      121 gatctgcctg aagatcgtcg acgaggtcaa gaaggccact ggccgccccg acctgaaggt
      181 ggcgctgcaa tcggtgacct cggcgaaccg catcccgctg ctgcagaacg gcaccatcga
      241 catcgagtgc ggctcgacca ccaacaacag cgagcgcggc aagcaggtgc agttcgcgac
      301 caactacttc tacaccggca cgcgctttct ggtgaagacc ggcacccagg tcaaatcgat
      361 cgacgacctg aagggcaaga ccatcgtctc gaccagcggc acgaccaact acaagatcct
      421 gcgcaacctc aacgaagaga agaagctcgg catcgacctg ctcgcggcga aggatcacgc
      481 cgaatcggcg ctgatggtcg agagcggccg cgcgctcgcg ttcggcatgg acgacatcct
      541 gctctacggc ctgcgcgcca gcagccagaa cccggctggg ctcgcggtgg tgggcgagcc
      601 gatccaggtc gagccctacg cgatcatgct gcggctggac gacccggcgt tcaagaagct
      661 cgtcgacgcc acgctggccg gcctgatgaa gagcggcgag ttcacgaagc tgtacacgaa
      721 gtggttcatg tcgccgatcc cgccgaaggg catcaacctg aacgcgccga tgagcaagga
      781 gctcgtcgac aacatgaagg cgctgagcga caaaccggcg acctgacaaa cacatgcccc
      841 gtgttacact gattcagtgt gaacgggggt ccttcatggc caacatgacg attgcggtcg
      901 acgaccagtt gctgcgcgcc gcccgcatca aggcggtgca gcagggcacg agcgtcaacg
      961 agatttgccg ccaggcgatc gaagccttcg cgcaaggcgc gggtgatgcc gacgcgcgca
     1021 tcgcccgcat caaggcgctc gcgaagaagg tgcgtgccgg cgacgaaccc ctgtggcccg
     1081 ggcgggatgc gctgtacgcc gaatcgcccg cgcgatgagc tgcttcttcg ataccaatgt
     1141 gctcgtctac gtgttcgatc gcgcggagcc cgcgaagcag gcgctcgcgc aggaacttgt
     1201 caccgagcac atgagcgcgc gcgacatggt gctgagcacg caggtgctgc aggagctcta
     1261 cgtcacgctg acgcgcaaga agcggctcgg caccgccgac gcgctcgaag tggtgaccac
     1321 cttcgcccag gaacgtgtcg tgcccgcgag cgccgatacc gtgctgcgcg gcctcgcgct
     1381 gagccagcgc caccagctct cggcgtggga cgcgctgatc ctgcaggccg ctctcgacgc
     1441 cggctgcacg acgctgttct ccgaagactt tcaggccggc gcgcgcttcg acgacctcgt
     1501 cgtcgtgaac ccgttcgcgc cgcaggtgca agcgccgcgc gcggcgtacg gcaagcggcg
     1561 ctgagcgcgc tgggcggttc gacaaacccc tgacgccggc aagcggcctg gctggttcgt
     1621 acactcgcgc tctgacccag gagttgtcat gtcgaacccg ccccgcctcg ccttgactgc
     1681 cgtcgcgctc acggcgctgc tcgccgcgtg ctcgaagccg gccgaagcgc cgccgcacgt
     1741 actgaacacg ccggcgccgc cggtcgccac gccgcctgcc gaccacgccg aatccggcat
     1801 tgcctggaag cacgccgcga gcgacgccga tgtcgacgcc gcgttcgcgc tggccaaggc
     1861 cgagaacaag ccggtgttcg tctactgggg cgccaagtgg tgcccgccgt gcaaccaggt
     1921 caaggcgacg ctgttcaacc gccaagactt catcgaacgc tcgcgcgcgt tcgtgccggt
     1981 ctacgtcgac ggcgacagcc cgggcgcgca gaagctcggg agccgcttca aggtcagcgg
     2041 ctacccgacg atgctgctct tcaacccgca gggcaccgag atgacgcggc tgccgggcga
     2101 ggtcgaggcc gagcgctaca cgcaggtgct gacgctgggg atgaacgcgc agcggcctgt
     2161 cagcgaagtg ctcgcacaag ccaaggccgg cggcgccggc atgggtgcga acgactggcg
     2221 cctgctcgcg ttctactcgt gggacaccga ccagcagctg gtgccgaagg gcggcgtgcc
     2281 ggcgctgctg aagcaactcg ccgatgcctg cccggccgat cagaacgaga ccgccacccg
     2341 cctgcgcctg aaggcgctcg ccgcggccga cgccaacgcg ccggccaagc ccgacccggc
     2401 cgcccaggcg acactgctga aggtgctggc cgacgcgccg accgcgcgcg cctacctcga
     2461 cgtgctgacg aactacgcca ccgacatcac gcgcgcgctc agcgcgaaag gcacgccgca
     2521 gcgcgccgag ctggtcgcgg cattcaacgc cgcgctgaaa cgcttcgagg ccgacacgac
     2581 gctgtcgcgc gccgaccgcc tcggcgcgat cgccgcgcgc gtcgacctcg cgcgcatgga
     2641 tgcgcccaaa cgcgcggccg tgcagctgcc cgacgcgctc gtcgccgatg cgcgcgagca
     2701 cgcggcgcgc gccgaccgcg agatcaccga cggctacgag cgccaggccg tgatcacctc
     2761 ggccgcgcac ctgctcaccg acgtggggct ggtggccgag tccgacacga tgctgaagaa
     2821 cagcctggcc aagagccatt cgccctacta cctgatgctg gccctggcca gcaacgccaa
     2881 gaagcgcggc gacaccgccg acgcgctgca ctggtacgaa gaatcgttcg ccaagagcga
     2941 aggcccggcg acccggctgc agtggggcgc gggctacctg aacgcgctga tcgatcttgc
     3001 gccgcacgat gaggcccgca tcgagaaggc tgcgagccaa ctgttcgccg aggccgccaa
     3061 gcagcccgac gcgttctacg agcgcagcgg ccgctcgctg cagcgggtgg ccagcaagct
     3121 gcagggctgg aacaagggcg gcgcgcacgc cgcggcgatc aagcggctgc aggcgcagct
     3181 cgacggcgtc tgcgccgcgc tggtcgcgga cgacccgaag cgcgcgacct gcgccgcgct
     3241 gctcaagccc acggccaaga ccaccgcgtg aagccggcac gaccggtcgg tgtcacgcgg
     3301 ccgcgtgcgg cctggcgctt gccttagcat gcgcgccatg ccgaaacccc gagcccgcac
     3361 gcctgcgccg ccgctgccgt cgaccgcgtt cgaccggcag cgccgcgccg tgttggccgc
     3421 cgccgcggca gcgctgggcg ggtcggcgct gccgcgggcc gcggcggccg acgacgggcc
     3481 ggtgcttggc gtctggaccc acgcctacgc ggcgttcggc ccaccgaagt acccgcgcgg
     3541 cttcgcgcac ttcgagtacg tgaacccggc cgcgcccaag ggcggcacgc tctatctgcg
     3601 caacccggac cggcgcagca gcttcgacaa gttcaactac tacacgctga aaggcaactc
     3661 gccggggggc ctgcagatct tcatgttcga gccgctggcg atcctgtcgg gcgacgagcc
     3721 gcagaccatg tacggcctgc tcgccgaaga gatgctgatc gcgcccgaca aggcgtcgat
     3781 gacgatccgg ctgcatccca aggcgcgctt ctacaacggc gacccggtca ccgcggccga
     3841 cgtcaagcac agcttcgatt cgctggccgg caagttcgcg tcgccgacct tgcagagtca
     3901 gatggccgcg gtcggcgccg ccgtcgtgct cgacccgcgc acggtgcgct tcgacctgaa
     3961 ggagcgcaac accgacacgc tgttcctcgt cggcggcctg ccggtcttct cggccaagtg
     4021 gggcctggag cccgacggca agcgcaagcc cttcgaccag atcgtcaccg agtacccgat
     4081 cacgaccggg ccgtacacga tcgggctcgt cgactcgaac cgccgcatcg agttcgcgcg
     4141 caaccccgag tactgggcgc gcgacctgcc ggtgcggcgc ggcttcttca acttcgaccg
     4201 cgtcgtctac cgctactaca aggacaacga cgtcgcgcgc gaggccttcc gcgccggcga
     4261 gttcgacatc ctgaaggagt acagcgcgcg cagctgggtg cgtcagcacg ccggcccgaa
     4321 gtggcaaagc ggccagatca tcaaggaccc gttcccgacc gcgttcggcg agatgcagca
     4381 ggccttcgac ctcaacctgc gccgcccgaa gttccaggac ctgcgggtgc gccgcgcgat
     4441 cgacctgacc tacgacttcg agatggtcgt gaac
//
LOCUS       DASRDG010000166         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1409740, whole genome shotgun sequence.
ACCESSION   DASRDG010000166 DASRDG010000000
VERSION     DASRDG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409740"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VFA35_02700"
     CDS             <1..368
                     /locus_tag="VFA35_02700"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZT55106.1"
                     /translation="RGRLIFGSIGCASCHTPVLVTGRSPVKALSERRVDAYSDLLLHD
                     MGEARADICLGQATPAEFRTEPLMGLRLLKQFLHDGAASTIEQAIDLHAGEAQRSKER
                     FDKLKPAERAAVLHFLGSL"
     gene            586..>2184
                     /locus_tag="VFA35_02705"
     CDS             586..>2184
                     /locus_tag="VFA35_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZT55107.1"
                     /translation="MAARSGSGLLLAAWLSGAALAQSSSPFGNIAPPGGQPNRLPTSA
                     PQPTAPAQPAPAAVNVLARVEGRPITQDQYDRIVSPYLARLRAQMGPAFTDDVLKMAR
                     KNAFDELVRHEVLVIESQRQQVQVTEADTDSLLRQDPMLQTNGKFDPVKLQEFKLSPQ
                     SNYLQLLPRLQEYAAVAKLDKQVHDRFTPSRAEVRAEFEKRSAQVRFDFFPLQSRDVS
                     LEPESTEPEWHAYFAAHAADYVKRAQLRLRYYRLPLPAASDSTHAAAEKRAVERGQRM
                     ADSLRAGTLADSSAETRDTGLFDVDAPVIPGIGRPPELAAAVARADSLPSLRVLGSFL
                     MPDAVLVPVVVERHPRRPQTFLEALPSVKRSADTEKRKAAAEADRRGYYDANRERYRA
                     ARAALTRVTLKLSTYKPRDIPAAELDRWYPLHGHTLYGLPDSARGWLPPLTDSLRRII
                     RGRIERSERDGWASSTMQKLASEMRTTRNVAGTARANGAVAETLSVVRGGPQADSLFN
                     AAVVDTILTTAAEQRARVQGPHTFA"
BASE COUNT          314 a          804 c          772 g          294 t
ORIGIN      
        1 aacgcggacg gctgatcttc ggcagcatcg gttgcgcctc gtgtcacacg ccggtgctgg
       61 tcacgggacg ctcgccggtc aaggcgctca gcgagcgacg cgtggacgcg tactcggacc
      121 tgctgctgca cgacatgggc gaggcgcgcg ccgatatctg cctcggccag gcgacgccgg
      181 ccgagttccg gaccgagccg ctcatgggtc tccggctcct gaaacagttc ctgcacgatg
      241 gcgccgccag caccatcgag caggcgatcg acctgcatgc gggcgaggcg cagcgttcga
      301 aggagcgctt cgacaagctg aagccggcgg agcgcgcggc ggtgctgcac ttcctcggct
      361 cgctctgacg cttgcctcgg ggctccggcg gtcgtgtcgc agcgaccgcc gggcccgcgg
      421 cgagctgtgc gcgcggcctc ggtcggcgcc ggaggagatc cggttttcac cccgcgaagc
      481 cggggtgcta ggctcggccg ccccccggcc cgagtccccg acgccccaca tccgatcgaa
      541 gaccccgagc gcatgcccgt cgcttcccgc acattcgcac gacaattggc tgcacgttcc
      601 ggctccggcc tcctcctggc cgcgtggctg agcggcgccg cgctcgccca gtcctcgtcg
      661 ccgttcggca acatcgcccc tccgggtggc cagccgaacc gcctgcccac gagcgcgccg
      721 cagcccacgg cgccggcgca gcccgccccg gcggcggtca acgtgctcgc gcgcgtcgag
      781 ggccgcccga tcacgcagga ccagtacgac cgcatcgtca gcccgtacct ggcgcggctg
      841 cgcgcccaga tgggcccggc gttcaccgac gacgtgctca agatggcgcg gaagaacgcg
      901 ttcgacgagc tggtccgcca cgaagtgctg gtgatcgagt cgcagcggca gcaggtgcag
      961 gtgaccgagg cggacacgga ttcgctgctg cgccaggacc cgatgctgca gacgaacggc
     1021 aagttcgatc cggtcaagtt gcaggagttc aagctgagcc cgcagtcgaa ctacctgcag
     1081 ctgctgcccc ggctccagga gtacgccgcc gtcgcgaagc tcgacaagca ggtgcacgat
     1141 cgcttcacgc cgtcgcgcgc cgaggtgcgt gcggagttcg agaagcggag cgcgcaggtg
     1201 cggttcgact tcttcccgct ccagagccgc gacgtctcgc tcgagccgga gtccaccgag
     1261 ccggagtggc atgcctactt cgccgcgcac gcggctgact acgtgaagcg ggcgcagctg
     1321 cggctgcgct actaccggct gccgcttccg gccgccagcg actcgacgca cgcggcggcg
     1381 gagaagcgcg cggtcgagcg cggccagcgc atggcggatt cgctgcgcgc cggcacgctc
     1441 gcggactcct ccgccgagac gcgggacacg ggcctgttcg acgtggacgc gccggtcatc
     1501 cccggcatcg gtcgcccgcc cgagctggcc gccgcggtcg cgcgcgccga ctcgctgccg
     1561 tcgctgcgcg tactggggtc gttcctgatg ccggatgcgg tgctggtgcc ggtggtggtc
     1621 gagcgccacc cgcggcggcc gcagacgttc ctcgaggcac tgccgagcgt caagcgcagc
     1681 gccgacaccg agaagcgaaa ggccgcggcc gaggcggatc gccgcggcta ctacgatgcg
     1741 aatcgcgagc gctatcgcgc cgcgcgcgcg gcgctgacgc gcgtcacgct caagctctcg
     1801 acgtacaagc cccgcgacat cccggcagcc gagctcgacc gctggtatcc gctgcacggc
     1861 cacacgctct acggcctgcc ggactccgcg cgcggctggc tcccgccgct caccgattcg
     1921 ctgcgtcgca tcatccgcgg gcggatcgag cggagcgagc gcgacggctg ggcgagcagc
     1981 acgatgcaga agctcgcatc ggagatgcgc accacgcgca acgtggccgg gaccgcgcgt
     2041 gcgaacggcg cggtcgcgga gacgctgtcg gtggtccgcg gcggcccgca ggcggattcg
     2101 ctgttcaacg cggccgtggt ggatacgatc ctcaccacgg cggctgagca gcgcgcacgc
     2161 gtgcagggac cgcacacctt cgcc
//
LOCUS       DASRDG010000167         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_827964, whole genome shotgun sequence.
ACCESSION   DASRDG010000167 DASRDG010000000
VERSION     DASRDG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827964"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFA35_02710"
     CDS             complement(<1..749)
                     /locus_tag="VFA35_02710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HZT55108.1"
                     /translation="MKLPTNTSPVGAALSVRNPWAMVPSLGNLDAYITAANRVPLLTH
                     DEEIAFATKLRATGDLEAAGKLVLSHLRLVVSISRKYLGYGLPHGDLIQEGNVGLMKA
                     VKRFDPGQGVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQSLK
                     DDAQDIDTHRSTLTQGEVDTLARQLNVKREDVLEMETRLSGGDVALEPQTDDSEESYA
                     PIAYLADESSEPTRVLEARDRDWLAGDGIALA"
     gene            1025..1942
                     /locus_tag="VFA35_02715"
     CDS             1025..1942
                     /locus_tag="VFA35_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HZT55109.1"
                     /translation="MFKNLIVYRVGPGWAANSAELEAGLDKMIFVPCGATQPVSAGWA
                     PPRGVAHGPLVESVGGQWLMKLVVEQRVLPSAVVKRRVDERAAQIEQATGRKPGKKQQ
                     KELKEEVMLELLPQAFTKQAAIKVWLDPKDRLLMIDASSPARADEVVTQLVKALDGLV
                     LTPLHTAQSPATAMSAWLTSTEAPAGFSVDRECELKSPDESRSVVRYARHALDIDEVR
                     GHIAGGKLPTQLALTWQGRVSLLLTEAMQIKKIAFLDGVFEGGAKPSKDEAFDADAAI
                     ATGELGQLIPDLIEALGGEQQPGVPALAA"
     gene            complement(1949..>2263)
                     /locus_tag="VFA35_02720"
     CDS             complement(1949..>2263)
                     /locus_tag="VFA35_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZT55110.1"
                     /translation="CPEATTSALHRAALKSPAAEHTALTNLFTGRPARGIVNRLMREL
                     GAISAAPSEFPLATAAIAALRAAAERAGSSDFSPLWSGQNARGCREVPAAQLTRELAA
                     GW"
BASE COUNT          340 a          759 c          811 g          353 t
ORIGIN      
        1 gccagcgcga tgccgtcgcc ggcgagccag tcgcggtcgc gtgcttcgag cacgcgggtc
       61 ggctcgctgc tctcgtcggc gaggtaggcg atcggcgcgt agctctcttc gctgtcgtcg
      121 gtctgcggct cgagcgccac atcgccgccg gacaggcgcg tttccatctc gagcacgtcc
      181 tcgcgcttga cattcaactg gcgggcgagc gtgtcgacct cgccttgcgt cagcgtgctg
      241 cggtgcgtgt cgatgtcttg cgcgtcgtcc ttcaggctct gcttcatcga gcgcaggttg
      301 aagaacagct tgcgctgggc cttggtcgtc gcgaccttga ccatgcgcca gttcttcagg
      361 atgtactcgt ggatctcggc cttgatccag tgcatcgcgt agctgaccag gcgcacgccc
      421 tggccgggat cgaagcgctt cacggccttc atcaggccga cgttgccttc ctggatcagg
      481 tcgccgtgcg gcaggccgta gcccaggtac ttgcgcgaga tcgaaaccac cagtcgcagg
      541 tgcgagagca cgagctttcc ggcggcttcg aggtcgccgg tggcgcgcag cttggtcgcg
      601 aacgcgatct cttcgtcgtg cgtcaacagc ggcacgcggt tggcggcggt gatgtaggcg
      661 tcgaggttgc cgagcgacgg caccatcgcc cacgggttgc gcacgctcag cgcggcgccg
      721 acaggggagg tgttcgtggg gagtttcatg gagagttcgg acacgcggac tcctttcaag
      781 ttccatcaat attagcactc tgaggttggg agtgctgatc gacctgggct gtcacgacgg
      841 cggtcgggcc gccacgcctc acgctgcgcc gcgcaacgct tttcaggtga gcaaacgctc
      901 acaacggcgc cgcaaagatc accgcgggta acggcggccg acgcggtccg ctttgcgtgg
      961 acaatcccgc ctccatcgcc cggcgagagc ctgccaggcg cattcagaca aggacgaggc
     1021 gaatttgttc aagaacctga tcgtgtaccg cgtcggcccg ggctgggccg ccaacagcgc
     1081 cgagctcgaa gccggcctcg acaagatgat cttcgtgccc tgcggcgcga cccagcccgt
     1141 ctcggccggc tgggcgccgc cgcgcggcgt ggcgcacggg ccgctggtcg agtccgtcgg
     1201 cgggcaatgg ctgatgaagc tggtggtcga gcagcgcgtg ctgccgtcgg cggtggtcaa
     1261 gcgccgcgtc gacgagcgcg ccgcgcagat cgagcaggcc accggccgca agcctggcaa
     1321 gaagcagcag aaggagctga aggaagaggt gatgctcgag ctgctgccgc aggccttcac
     1381 gaagcaggcc gcgatcaagg tctggctcga ccccaaggac cgcctgctga tgatcgacgc
     1441 gagcagcccg gccagggccg acgaggtcgt cacgcagctc gtgaaagcgc tcgacggcct
     1501 cgtgctgacg ccgctgcaca ccgcgcagtc gccggccacg gcgatgtcgg cgtggctgac
     1561 cagcaccgag gcgccggccg gcttcagcgt cgaccgcgaa tgcgagctga agtcgcccga
     1621 cgagagccgc tcagtggtgc gctacgcgcg ccacgcgctc gacatcgacg aggtgcgcgg
     1681 gcacatcgcg ggcggcaagc tgccgacgca gctcgcgctc acctggcagg gccgcgtctc
     1741 gctgctgctg accgaggcga tgcagatcaa gaagatcgcg ttcctcgacg gcgtgttcga
     1801 gggcggagcc aagccgtcga aggacgaggc cttcgacgcc gatgccgcga tcgcgaccgg
     1861 cgaactcggc caactgatcc ccgacctgat cgaggcgctc ggcggcgagc agcaaccggg
     1921 cgtgccggcc ctcgcggcct gaaaccgact accagcccgc ggcgagttcc cgggtgagct
     1981 gcgctgcggg gacctcgcgg cagccgcgtg cgttctgccc cgaccacagc ggcgagaagt
     2041 cgctgctgcc ggcgcgctcg gcggccgcgc gcagcgccgc gatcgcggcg gtcgcgagcg
     2101 ggaactcgga cggcgccgcg ctgatggcgc cgagttcgcg catcagccgg ttgacgatgc
     2161 cgcgcgccgg gcggccggtg aacaggttgg tcagcgcggt gtgctcggcg gccgggctct
     2221 tcagcgcggc gcggtgcagc gcgctcgtcg tcgcctccgg gca
//
LOCUS       DASRDG010000168         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_258229, whole genome shotgun sequence.
ACCESSION   DASRDG010000168 DASRDG010000000
VERSION     DASRDG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258229"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..188
                     /locus_tag="VFA35_02725"
     CDS             9..188
                     /locus_tag="VFA35_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HZT55111.1"
                     /translation="MLQRKFSVAAASKAASRVCACATAIALLAACGQKGPLRLPEPAT
                     GTAAPASSAASAARP"
     gene            185..1456
                     /gene="lysA"
                     /locus_tag="VFA35_02730"
     CDS             185..1456
                     /gene="lysA"
                     /locus_tag="VFA35_02730"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZT55112.1"
                     /translation="MTQPGSPHIAYRGDDLFIEGRSVAALAREHGTPLYAYAQTAMLD
                     ALASYQRALVGRDHLICYAMKANSNLAVIQTFARAGCGFDIVSGGELERVLAVGVPGS
                     KIVFSGVGKTRAEMKRALEVGVQCFNVESEAELALLSQVAAASGATARVSLRINPDVD
                     AGTHPYISTGLKGNKFGIAHEDALANYLRAAELPGLEVVGIDCHIGSQITDAGPYLDA
                     LDRLLDLIETIEARGVTIHHLDLGGGLGITYTDEAPPSAEALVTLLLERIDARGHGHR
                     KIMFEPGRSLVGNAGVLVSEVLYVKPGEQKNFCIVDAAMNDLMRPAMYQAWMKIVACH
                     ARPEPPMLFDVVGPVCESGDWLGKDRELAVRTGDFIAVLSAGAYSMSMASNYNTRGRA
                     AEVMVSGDQAWLIREREPVESLFAGEHLLPG"
     gene            complement(1453..2499)
                     /locus_tag="VFA35_02735"
     CDS             complement(1453..2499)
                     /locus_tag="VFA35_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZT55113.1"
                     /translation="MSAVPQSATSTPLVEVHDLAKTFDVSAPWLNRVVERAPRQFVHA
                     VDGISFSIRRGKTLALVGESGCGKSTVARLLVGLYAPTRGRVEFDGIDTATTFRSTGA
                     LALRRRMQMIFQDPYASLNPRWKVADIVGEPLKEHGLLQHASGSGAKADKNETKARVA
                     QLLQSVGLAAADVEKFPHQFSGGQRQRISIARALATQPEFLVCDEPTSALDVSVQAQV
                     LNIMKDLQRKQGLTYLFISHNLAVVRHVSDQVGVMYLGRIVELADKAELFARPRHPYT
                     RMLLDAIPDIHMSGRARTPVQGEVPNPLNPPSGCSFHPRCPHANARCKAERPALKMFG
                     GALIACHAVEEGRI"
     gene            complement(2496..>2637)
                     /locus_tag="VFA35_02740"
     CDS             complement(2496..>2637)
                     /locus_tag="VFA35_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HZT55114.1"
                     /translation="PAGCAFNPRCPRVFDRCRVERPDLLPAGATRAACWLYAPALREA
                     VQ"
BASE COUNT          401 a          934 c          913 g          389 t
ORIGIN      
        1 tccagaggat gttgcagcgc aagttcagtg tagcggcggc ctcgaaggct gcgtcgagag
       61 tgtgtgcatg cgcgaccgcc atcgcgctgc tcgcggcctg cggccagaag ggcccgctgc
      121 gcttgccgga gccggcaacc ggcacggctg cgccagcttc ctccgccgcc tccgccgccc
      181 gaccatgacc cagcccggct ctccgcacat cgcctaccgc ggcgacgacc tgttcatcga
      241 gggccgctcg gtcgccgcgc tcgcgcgcga gcacggcaca ccgctgtacg cctacgcgca
      301 gaccgcgatg ctcgatgcgc tcgccagcta ccagcgcgcg ctcgtgggcc gcgatcacct
      361 gatctgctac gcgatgaagg cgaactcgaa cctcgcggtg atccagacct tcgcgcgggc
      421 cggctgcggc ttcgacatcg tctcgggcgg cgaactcgag cgcgtgctcg cggtcggcgt
      481 gccgggctcg aagatcgtgt tctccggcgt cggcaagacg cgcgccgaaa tgaagcgcgc
      541 gctcgaggtc ggcgtgcagt gcttcaacgt cgagagcgag gccgaactgg ctctgctgtc
      601 gcaggtcgcg gcggccagcg gcgcgaccgc gcgcgtgagc ctacgcatca accccgatgt
      661 cgatgccggc acgcatccct acatctcgac cggcctgaaa ggcaacaagt tcggcatcgc
      721 gcacgaagat gcgctggcga actacctgcg cgcggccgaa ctgccgggcc tggaggtggt
      781 cggcattgac tgccacatcg gctcgcagat caccgacgcc gggccgtacc tcgacgcgct
      841 cgaccgcctg ctcgacctca tcgagacgat cgaggcgcgc ggcgtcacga tccaccacct
      901 cgacctcggc ggcggcctgg gcatcaccta caccgacgag gcgccgccct cggccgaagc
      961 gctcgtgacg ctcctgctcg agcgcatcga cgcgcgcggc catggccacc gcaagatcat
     1021 gttcgagccg ggccgctcgc tggtcggcaa cgccggcgtg ctggtcagcg aggtgctgta
     1081 cgtgaagccc ggcgagcaga agaacttctg catcgtcgac gccgcgatga acgacctgat
     1141 gcgcccggcg atgtaccagg cctggatgaa gatcgtcgcg tgccatgcgc gccctgagcc
     1201 gccgatgttg ttcgatgtcg tcggcccggt ctgcgaatcg ggcgactggc tcggcaagga
     1261 ccgcgagctc gcggtgcgca ccggcgactt catcgcggtg ctgtcggccg gcgcgtacag
     1321 catgagcatg gccagcaact acaacacgcg cggccgcgcc gccgaggtga tggtgtcggg
     1381 cgatcaggcc tggctgatcc gcgagcgcga gccggtcgag tcgctgttcg ccggcgagca
     1441 cttgctgccg ggctagattc gcccctcttc gaccgcgtgg cacgcaatca gcgcgccacc
     1501 gaacatcttc agcgccggcc gctcggcctt gcagcgcgcg ttcgcatgcg ggcagcgcgg
     1561 atggaagctg cagccgctcg gcgggttcag cgggttcggc acctcgccct gcactggcgt
     1621 gcgggcgcgg ccgctcatgt ggatgtcggg gatcgcgtcg agcagcatgc gcgtgtacgg
     1681 gtgccgcggg cgcgcgaaca gctcggcctt gtcggcgagt tcgacgatgc ggccgaggta
     1741 catcacgccg acctgatcgc tcacatggcg cacgaccgcg aggttgtgcg agatgaacag
     1801 ataggtcagg ccctgcttgc gctgcaggtc cttcatgatg ttcaggacct gggcctgcac
     1861 cgagacgtcg agcgccgagg tcggctcgtc gcagaccagg aactcgggct gcgtcgcgag
     1921 cgcgcgcgcg atcgagatgc gctggcgctg gccgcccgag aactggtgcg ggaacttctc
     1981 gacatcggcc gccgcgagcc cgaccgactg caaaagctgc gcgacccgcg ccttcgtctc
     2041 gttcttgtcg gccttggcgc ccgaaccgct ggcgtgctgc agcaagccat gctccttcag
     2101 cggttcgccg acgatgtcgg ccaccttcca gcgcgggttc aggctcgcgt acggatcctg
     2161 gaagatcatc tgcatgcgcc ggcgcagcgc gagcgcgccg gtcgacctga acgtggtcgc
     2221 ggtgtcgatg ccgtcgaact cgacgcggcc gcgcgtcggc gcgtacaagc cgacgagcag
     2281 ccgcgcgacg gtgctcttgc cgcagcccga ctcgcccacg agcgcgagcg tcttgccgcg
     2341 ccggatcgag aagctgatgc cgtcgaccgc atgcacgaac tggcgcggcg cgcgctcgac
     2401 cacgcggttg agccacggcg ccgacacgtc gaaggtcttc gccaggtcgt gcacctcgac
     2461 caagggcgtg ctcgtcgcgg actgcggcac ggcgctcact gcacagcctc ccgcaacgcc
     2521 ggcgcataca gccagcacgc cgcacgggtc gcgccggcgg gcagcagatc gggccgctcg
     2581 acccggcagc ggtcgaacac acgcgggcag cgcgggttga acgcgcagcc ggcggga
//
LOCUS       DASRDG010000169         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1057526, whole genome shotgun sequence.
ACCESSION   DASRDG010000169 DASRDG010000000
VERSION     DASRDG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057526"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..2399)
                     /locus_tag="VFA35_02745"
     CDS             complement(84..2399)
                     /locus_tag="VFA35_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT55115.1"
                     /translation="MSKAQRWAWLISTVAATGAGLVLAFLLSIATNNPTLYERHYVWL
                     FWVNVTVATLLVLVIGIAGVRLLLRVKRGKFGSRLLLKLAAIFALVGVVPGVLIYTVS
                     YQFVSRSIESWFDVKVESALDAGLNLGKGTLDAIVGDVATKTRLAAERLGETPGSAQS
                     LALERLREQLSAQDIAIVDKTGQTVLSTSGSTAAHLMPERPSATLLRQARNQRVVSEL
                     VGLDEDAAASGSANNARVRALAFIPTTTFALGEDERYLLVNQLLPRALAANALAVQAA
                     YREYQQRALQRGGLRKMYIGTLTLALVLAVFGALLLAATLGNQLARPLLLLAEGMRQV
                     AGGDLSAKPVFASRDELGGLTRSFADMTTQLAEARELVQRSVVQVEGARANLQTILDN
                     LTAGVIVFDRHGNIDTVNPGATRIMRLPLSAYRGQRLAAVPGLAEFAQAVGQRFELHS
                     TSPEAGERDHWQDSFELQTAAQPGRGQALERASITLLVRGAAMPQGATLMVFDDITEL
                     VSAQRSEAWSEVARRLAHEIKNPLTPIQLSAERLQHKLGPKLEGADQAMLARSVDTIV
                     NQVQAMKTLVNEFRDYARLPAANLMPIDLNALVAEVLGLYGAAQEAGQLHAELAPALP
                     QIVGDSTQLRQVIHNLVQNALDAVADQPDGRVRVSTEVARTEQGEPRAVRLQVIDNGP
                     GFSDKVLKRAFEPYVTTKSKGTGLGLAVVKKIADEHGARVRIANLGSLGEDGAGGAPT
                     RGAQVSLSFAKFAPMPGTPHSTTAPATTGAH"
     gene            complement(2512..>3041)
                     /locus_tag="VFA35_02750"
     CDS             complement(2512..>3041)
                     /locus_tag="VFA35_02750"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HZT55116.1"
                     /translation="LLALALLLPSLPAVRAAELELSGFELTHGEDGVFLSYAVNLELS
                     RSVDEALGKAVPLFFVAEAELFRNRFYWRDQRIGYAIRRWRIVFQPLTSTYRVTFDGG
                     LSQNYATRAEALAAISRSARWKIAEGPQIDENSRQYLEFSFKLDTSQLPRPMQIGIGG
                     EKDWAMSVQRMQKIN"
BASE COUNT          409 a         1057 c         1110 g          465 t
ORIGIN      
        1 ctcagccggc gccgggccgc cccaaggcgg ctgagcgccc cctcgggggg caggaacgaa
       61 gtgacgtggg ggtgctcatg tgttcagtgc gcgccggtgg tggcaggagc cgtcgtggaa
      121 tggggggtgc cgggcatcgg cgcgaatttc gcgaatgata gcgagacttg cgcaccccgg
      181 gtcggcgccc cgccggcgcc gtcttcgccg aggctgccga ggttggcgat gcgcacgcgc
      241 gcgccgtgct cgtcggcgat cttcttgacg accgccaggc ccaggccggt gcccttgctc
      301 ttggtcgtca cgtagggctc gaaggcgcgc ttgagcacct tgtcggagaa gcccggcccg
      361 ttgtcgatca cctgcagccg cactgcgcgc ggctcgccct gctcggtgcg cgcgacctcg
      421 gtgctcacgc gcacccggcc gtcgggctgg tccgcgacgg catcgagcgc gttctgcacg
      481 aggttgtgga tcacctggcg cagttgggtg ctgtcgccga cgatctgcgg cagcgccggc
      541 gccagctcgg cgtgcagttg cccggcctcc tgcgccgcgc cgtacaggcc gagcacctcg
      601 gcgaccagcg cgttcaggtc gatcggcatc aggttggcgg ccggcagccg cgcgtagtcg
      661 cggaactcgt tgaccagggt cttcatcgcc tgcacctggt tgacgatggt gtcgaccgag
      721 cgcgccagca tcgcctggtc ggcgccttcg agcttcgggc cgagcttgtg ctgcaggcgc
      781 tcggccgaca gctggatcgg cgtcagcggg ttcttgatct cgtgcgcgag cctgcgcgcg
      841 acctcgctcc aggcctcgct gcgctgggcc gagacgagct cggtgatgtc gtcgaagacc
      901 atcagcgtcg cgccctgcgg catcgcggcg ccgcgcacga gcagcgtgat cgacgcccgc
      961 tccaacgcct gcccgcggcc cggttgggcc gcagtctgca gctcgaacga atcctgccag
     1021 tggtcgcgct cgccggcctc ggggctggtg ctgtgcagct cgaagcgctg gcccacggcc
     1081 tgcgcgaatt cggccaggcc cggcaccgcg gccaggcgct gaccgcggta ggccgagagc
     1141 ggcagccgca tgatgcgggt cgcgcccggg ttcacggtgt cgatgttgcc gtggcggtcg
     1201 aacacgatca cgccggcggt caggttgtcg aggatggtct gcaggttcgc gcgcgcgccc
     1261 tcgacctgca cgacgctgcg ctgcaccagt tcgcgcgcct ccgcgagctg ggtcgtcatg
     1321 tcggcgaacg agcgcgtcag gccgccgagc tcgtcgcgcg acgcgaatac cggcttggcc
     1381 gacaggtcgc cgccggcgac ctgtcgcatg ccttcggcca gcagcagcag cggccgcgca
     1441 agctggttgc cgagcgtcgc agcgagcagc agcgcgccga acaccgcgag cacgagcgcg
     1501 agcgtcagcg tgccgatgta catcttgcgc aggccgccgc gctgcagcgc gcgctgctgg
     1561 tattcgcggt aggcggcctg caccgcgagc gcgttcgccg cgagcgcgcg cggcagcagc
     1621 tggttgacga gcagatagcg ctcgtcttcg ccgagcgcga aggtcgtggt cgggatgaac
     1681 gcaagcgccc gcacgcgcgc gttgttggcg ctgccgctgg cggcggcgtc ctcgtccagg
     1741 cccaccagct cgctgaccac gcgctggttg cgcgcctggc gcagcagcgt cgccgagggc
     1801 cgctcgggca tcaggtgcgc ggcggtgctg ccgctcgtgc tcagcacggt ctgcccggtc
     1861 ttgtcgacga tcgcgatgtc ctgcgccgag agctgctcgc gcaggcgctc gagcgcgagc
     1921 gactgcgccg agccgggcgt ctcgccgagc cgctcggcgg ccagccgcgt cttggtcgcg
     1981 acatcgccga cgatcgcgtc gagcgtgccc ttgccgaggt tcagcccggc gtcgagcgcg
     2041 ctctcgacct tgacgtcgaa ccagctctcg atgctgcgcg agacgaactg gtagctcacc
     2101 gtgtagatca gcacgccggg cacgacgccg acgagcgcga agatcgccgc gagcttgagc
     2161 agcagccggc tgccgaactt gccgcgcttc acacgcagca gcaggcgcac gccggcgatg
     2221 ccgatgacga gcacgagcag cgtggcgacc gtgacgttga cccagaacag ccagacgtag
     2281 tggcgctcgt acagcgtcgg gttgttggtc gcgatcgaga gcaggaacgc gagcaccagg
     2341 ccggcgccgg tggcggcgac ggtggagatc agccaggccc agcgctgcgc tttgctcatt
     2401 ggtgcccccc gttcgccgag tacggcgccc ggccccccgg gggggcggag cccggcttgg
     2461 ggcagcccgg cgctcgggct cccccggcat ccacgatcgg cttcatctcg gtcagttgat
     2521 cttctgcatg cgctggaccg acatcgccca gtccttctcg ccgccgatgc cgatctgcat
     2581 cgggcgcggc agctgcgagg tgtcgagctt gaagctgaac tcgaggtact ggcggctgtt
     2641 ctcgtcgatc tgcgggccct cggcgatctt ccagcgcgcg ctgcggctga tcgcggcgag
     2701 cgcctcggcg cgggtcgcgt agttctggct caggccgcca tcgaaggtga cacggtaggt
     2761 cgatgtcagc ggctggaaca cgatgcgcca gcgccggatc gcgtagccga tgcgctggtc
     2821 gcgccagtag aagcggttgc ggaacagctc ggcctcggcg acgaagaaca gcggcacggc
     2881 cttgccgagc gcctcgtcga cgctgcgcga gagctccagg ttcaccgcgt agctgaggaa
     2941 gacgccgtcc tcgccgtgcg tcagctcgaa gccactcagt tcgagctcgg ccgcgcgcac
     3001 ggcgggcagc gacggcagca gcaaggccag cgccagcagc a
//
LOCUS       DASRDG010000170         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_828608, whole genome shotgun sequence.
ACCESSION   DASRDG010000170 DASRDG010000000
VERSION     DASRDG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828608"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1730)
                     /locus_tag="VFA35_02755"
     CDS             complement(63..1730)
                     /locus_tag="VFA35_02755"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZT55117.1"
                     /translation="MKSIFLAFAALYATAGFAQAADDAVQRAMNDELRRSMQSLQLPG
                     EAKPYFIAYQVEDIHLLDVQASLGAVGFSGERGWRYARVDVRVGDARLDSSRMAGRQP
                     IFVELPVEDDYDGVRRALWLATDRAYKRALEDLAAKRGVLAAHDEPDRQPDQTPAPML
                     HAIGADAMPTRFDRAGTEAKLRELSRVFADARDLEQSQVWLSQRMRHVWFANSEGSRI
                     EKRVPEVQFGAAARARADDGLSVWDYDALSANDLAQLPAQADLAARLRALAQRVSAQR
                     KAVRLADDYTGPVLFTGDAAANLFGGHFAPQLWPAPRQYSNQAISAAGGSFERRIGSP
                     VLPPGVSVSDDPLRADFRGEALLSAFNVDDDGVAAAKKELIVNGRLKQVLCSRAPVGK
                     NCNAGNNRGAAAAGSTLELVSEHPKTETALRSELARAAHDAELPYALEVERLFSSDAL
                     QRLVFGSQPTWMGSGDQGEVVLRATLRYADGHGVPVRNVRLGNVDLRDFRTVVGIGDA
                     PALYAKGFGDRQIAFLVPPLLFEELTASDDRSGTVKPPLLKSPLAAP"
     gene            complement(1732..>2277)
                     /locus_tag="VFA35_02760"
     CDS             complement(1732..>2277)
                     /locus_tag="VFA35_02760"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZT55118.1"
                     /translation="NFLLGRTPIDGFAHSNGHGRSQSGFRPVSRQSNMIVGVEKPGDY
                     DALKKRLLAEIAEQGKPWGLIIRDLDGGFTETARASTGGAKLLPTMVYRLYADGREEL
                     VRGVDLVGTPLTLLAQIEAADGRFGVFHGWCGAESGYVPVSTVAPDLLIRRIEVQRKP
                     KSQERPPILPAPGTAANAEGR"
BASE COUNT          352 a          782 c          791 g          352 t
ORIGIN      
        1 ttcatcgagc ccgcttgcgt cgaatctgga agatcaaaaa ccgggcgcga tgaatcgcgc
       61 ccctacggcg ccgccagggg actcttgagc aacggcggct tcaccgtgcc gctgcgatca
      121 tcgctcgcgg tcagttcctc gaacagcagc ggcggcacga ggaacgcgat ctgccggtca
      181 ccgaaaccct tggcgtacag cgcgggcgcg tcgccgatgc cgacgacggt gcggaagtcg
      241 cgcaggtcga cgttgccgag gcgcacgttg cgcaccggca cgccgtgccc gtcggcgtag
      301 cgcagcgtcg cgcgcagcac gacctcgccc tggtcgccgc tgcccatcca cgtcggctgg
      361 ctgccgaaga cgaggcgctg cagcgcgtcg ctgctgaaca ggcgttcgac ttcgagcgca
      421 tacggcagct cggcgtcgtg cgcagcgcgc gcgagttcgc tgcgcagcgc ggtctcggtt
      481 ttcggatgtt cgctgacgag ctccagcgtc gaacccgcgg cggcggcgcc gcgattgttt
      541 ccggcgttgc aattttttcc gaccggtgcg cgcgagcaga gcacctgctt caagcgaccg
      601 ttgacgatga gctccttctt cgccgcggcg acgccgtcgt cgtcgacatt gaacgcggac
      661 aacagcgcct ctccgcggaa atccgcacgc agcggatcgt cgctgaccga cacgcccggc
      721 ggcagcacgg gtgagccgat gcgacgctcg aagctgccgc ccgccgcgct gatcgcctga
      781 ttggaatact ggcgcggcgc gggccagagc tgcggcgcga aatgaccgcc gaacaggttg
      841 gccgcggcgt cgcccgtgaa cagcaccggg ccggtgtagt cgtcggcgag gcgcaccgcc
      901 ttgcgctgcg cgctcacacg ctgcgcaagc gcgcgcaggc gcgcggcgag atcggcctgc
      961 gccggcaact gggcgaggtc attcgcggac aaggcgtcgt agtcccacac cgagaggcca
     1021 tcgtcggcgc gcgcgcgcgc cgcggcgccg aactgcactt cgggcacgcg cttctcgatg
     1081 cggctgccct cgctgttggc gaaccagacg tggcgcatgc gctgcgacag ccagacctgc
     1141 gactgttcga ggtcgcgtgc gtctgcgaac acacgcgaga gctcgcgcag cttcgcttcg
     1201 gtgccggcac gatcgaagcg cgtcggcatc gcgtcggcac cgatcgcgtg cagcatcggc
     1261 gccggcgtct gatcgggctg gcgatcgggc tcgtcgtgcg cggcaagcac gccgcgcttg
     1321 gccgcgaggt cttcgagcgc acgcttgtac gcgcggtcgg tggcgagcca cagcgcgcgc
     1381 cgcacgccgt cgtagtcgtc ctcgaccggc agttcgacga agatcggctg gcgtccggcc
     1441 atgcggctcg aatcgagcct ggcatcaccg acacgcacat ccacgcgcgc atagcgccag
     1501 ccgcgttcgc cggaaaagcc caccgcgccg agcgaggcct gcacgtcgag caagtggatg
     1561 tcctcgacct ggtaggcgat gaagtacggc ttcgcctcgc ccggcagttg caggctctgc
     1621 atcgagcgcc gcagctcgtc attcatcgcg cgctgcacgg catcgtcggc ggcctgggcg
     1681 aagccggccg tggcgtagag ggcggcaaag gcaagaaata tacttttcat ttcaacgacc
     1741 ctccgcgttc gctgccgtgc ccggcgcagg caggatcggc gggcgctcct gcgatttggg
     1801 cttgcgctgc acctcgatgc gacggatcag caggtcgggc gccacggtcg acaccggcac
     1861 gtagccggac tcggcgccac accagccgtg gaacacgccg aagcgcccgt ccgcggcttc
     1921 aatctgggca agcagggtca gcggcgtgcc gaccagatcg acgccgcgca cgagttcctc
     1981 gcggccgtcg gcatacaggc ggtagaccat cgtcggcagc agcttggcgc cgccggtgct
     2041 ggcgcgcgcg gtctcggtga agccgccgtc gagatcgcgg atgatcaggc cccagggttt
     2101 gccttgctcg gcgatttcgg cgagcaggcg tttcttcagc gcgtcgtagt cacctggttt
     2161 ttccacgccg acaatcatgt tcgattggcg cgacacggga cggaagccgg actgcgagcg
     2221 gccatggccg ttcgagtgcg cgaagccgtc gatcggcgtg cgcccgagca ggaaatt
//
LOCUS       DASRDG010000171         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_730713, whole genome shotgun sequence.
ACCESSION   DASRDG010000171 DASRDG010000000
VERSION     DASRDG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730713"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..639
                     /locus_tag="VFA35_02765"
     CDS             13..639
                     /locus_tag="VFA35_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HZT55119.1"
                     /translation="MLGFEERAEWRALSGTVRRRREWLFGRAAVKEAVRMVLFQQTGT
                     LLYPSDITVLHDDLGAPFVDGWWRGELAEAPQVSLSHTARACLVAVAAAESPVGVDFE
                     DLGRIQQPELMIGMLTAGERATVEGLAGPALDERLLRLWCAKEAAAKYLGVGLQGQPE
                     AFEVGFVDDACARARVVFEGATTEVRIVRDGAAVIAVAAGEPAAVEVH"
     gene            639..923
                     /locus_tag="VFA35_02770"
     CDS             639..923
                     /locus_tag="VFA35_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZT55120.1"
                     /translation="MDRFTQDTAEKTVCLIVEDLVQDWGLDLAAPVGPTTMLVADLDF
                     ASVDIIQLCVALEQSYERKLGFHDLLMKDGSYVGDLSLAQVAAHVESRLQ"
     gene            941..>2007
                     /locus_tag="VFA35_02775"
     CDS             941..>2007
                     /locus_tag="VFA35_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZT55121.1"
                     /translation="MTVRTLFIGMDGATFTVLNDLTARTGEGPVMPFLAKIFEEGTRA
                     KLRSTPNPLTPPAWVSLMTGRGPGHHGVFDFIRAEERAEDVFFTLYDSRDCRVETIWS
                     IASRQGLKVAALNFPFTAPPPKDLNGFMVPGFIPWRHLRRNSAPHDLYDRLKAIPEFD
                     AKELAWDFEQEKQAMEELTHEDRENWVKYHLPRERQWFRIADHLLREEAPDLMAVMFD
                     GVDKLQHQAWLYVDPKQQSGELSDYHKRMRVLCLDYFRQLDGFIEKLVTAAGPEVQVF
                     LASDHGFTATTEVVRINAYLHEKGYLKWKEVPDTDAGRRREDSMFAFLDWQHTVAYCR
                     TPSSNGINIRVARHPGESGVPP"
BASE COUNT          325 a          687 c          683 g          312 t
ORIGIN      
        1 ctggcgcacg ccttgctcgg cttcgaggag cgggccgaat ggcgcgcgct ttcgggcacg
       61 gtgcggcgca ggcgcgagtg gctgttcggc cgggcggccg tcaaggaggc ggtgcggatg
      121 gtcctgttcc agcagacggg aacgcttctc tatccatccg acatcacggt gctgcacgac
      181 gatctcggcg cccccttcgt cgacggctgg tggcgcggcg agctggccga ggcgccgcag
      241 gtgtcgctct cgcacaccgc acgcgcctgt ctggtggccg tcgccgccgc cgagtcgccg
      301 gtgggcgtcg acttcgagga cctgggccgg atccagcagc ccgagctcat gatcggcatg
      361 ctcaccgccg gcgagcgtgc cacggtcgaa ggcctggccg gccccgcgct cgacgagcgg
      421 ctgcttcgcc tctggtgcgc caaggaagcg gcggcaaagt acctgggcgt cggcctgcag
      481 ggccagcccg aagccttcga ggtcggcttc gtcgacgacg cctgcgcccg cgcgcgggtc
      541 gtcttcgagg gcgcgacgac cgaagtccgg atcgtccgcg acggcgccgc ggtgatcgca
      601 gtggccgcgg gcgagcccgc cgcggtagag gttcattgat ggacagattc actcaggaca
      661 cggcggagaa gacggtctgc ctcatcgtcg aggacctggt gcaggactgg ggcctagacc
      721 tcgccgcgcc ggtcgggccg acgacgatgc tggtggccga cctcgacttc gcgtcggtcg
      781 acatcatcca gctgtgcgtc gcgctcgagc agagctacga gcgcaagctc ggcttccacg
      841 acctgctgat gaaggacggc agctacgtcg gcgacctgtc gctggcccag gtggcggcgc
      901 acgtcgaatc cagattgcaa tagggaagag ggagctcgaa atgaccgttc gcacgctatt
      961 catcggcatg gacggtgcca cgttcacggt cctgaacgac ctgacggcac gcaccggcga
     1021 aggcccggtg atgcctttcc tggcgaagat cttcgaggag ggcacccgcg ccaagctgcg
     1081 ctcgacaccg aacccgctga cgccgccggc ctgggtgtcg ctgatgaccg ggcgcggccc
     1141 ggggcatcac ggcgtgttcg acttcatccg cgccgaggag cgcgccgaag acgtcttctt
     1201 cacgctctac gactcgcgcg actgccgggt cgagaccatc tggtcgatcg ccagccgcca
     1261 gggcctgaag gtcgcggcct tgaacttccc gttcaccgcg ccgccgccga aggatctgaa
     1321 cggcttcatg gtgcccggct tcatcccctg gcgccacctg cgccgcaatt cggcgccgca
     1381 cgacctttac gaccgcctca aggcgattcc cgaattcgac gccaaggagc tcgcctggga
     1441 cttcgagcag gaaaagcagg cgatggagga gctcacccac gaggatcgcg agaactgggt
     1501 gaagtaccac ctgccgcgcg agcgccagtg gttccgcatc gccgaccacc tgctgcgcga
     1561 ggaagcgccg gacctgatgg cggtgatgtt cgacggcgtc gacaagctgc agcaccaggc
     1621 ctggctctat gtcgatccga agcagcagtc gggcgagctg agcgactatc acaagcgcat
     1681 gcgcgtgctt tgcctcgact acttccgcca gctcgacggc ttcatcgaga agctggtcac
     1741 cgccgccggc cccgaggtgc aggtgttcct tgcctccgac cacggcttca ccgccaccac
     1801 cgaggtcgtg cgcatcaacg cctacctgca cgagaagggc tacctgaagt ggaaggaggt
     1861 gcccgacacc gacgccggcc ggcgccgcga agacagcatg ttcgccttcc tcgactggca
     1921 gcacacggtc gcctattgcc gcacgccgtc gagcaacggc atcaacattc gggtggcgcg
     1981 ccatccgggc gagtcgggcg tgccgcc
//
LOCUS       DASRDG010000172         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_596851, whole genome shotgun sequence.
ACCESSION   DASRDG010000172 DASRDG010000000
VERSION     DASRDG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596851"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1274)
                     /locus_tag="VFA35_02780"
     CDS             complement(171..1274)
                     /locus_tag="VFA35_02780"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZT55122.1"
                     /translation="MIPETVPLPDPFPLYRGGWLGAARIAYESWGRLNAARDNAILLF
                     TGLSPSAHAAATAADPTPGWWQPMIGPALAIDTNRYFVVCVNSLGSCFGSTGPASIDP
                     ATGERYRLDFPEIAVEDIASAGFEAVRALGIERLAVVMGSSLGGSLAVAFAALHPQGA
                     RRLVSISGSAAASPFAIALRSVQREAVMRDPDWQGGHYADDKPPRNGMTVARKLGTIT
                     YRSATEWRQRFGRAPLAGLALDSDPFGPRFAVEGYLEAQAERFIRVYDPNCYLYLSRA
                     MDRFDLAEHGGSYQAALAASGRDGGLEQALVLGVESDFLYPIHEQAAIAETLQQVGVR
                     TRFRRLPSIQGHDAFLVDIPRFDAEIRAFLAQP"
     gene            complement(1330..2271)
                     /gene="cysK"
                     /locus_tag="VFA35_02785"
     CDS             complement(1330..2271)
                     /gene="cysK"
                     /locus_tag="VFA35_02785"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZT55123.1"
                     /translation="MIYENILATIGRTPVVRIQRLAPQGVTMYVKCEFFNPMASVKDR
                     LAIAIIEDAERSGALKPGQTVVEATSGNTGIALAMVCAAKGYPFVGVMAETFSVERRK
                     IMRMLGAKVILTPAAERGQGMVRKAAELAKKNGWFLARQFENPANPAYHRNTTGPEIL
                     QDFVGRKLDFFVTGWGTGGTLTGAGEVIRLARPEVKIVVAEPEAAALLSGKPFSPHKI
                     QGWTPDFVPAVLNREVAHRILTVTDAEAISTSRELASKEGIFVGISSGATFAAALKVA
                     REAGAGASILAMLPDTAERYLSTPLFEGIPDGSDPET"
     gene            2377..>2761
                     /locus_tag="VFA35_02790"
     CDS             2377..>2761
                     /locus_tag="VFA35_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZT55124.1"
                     /translation="MNFHQLQYAVAIAHHNLSVTQAASALGTSQPAISRALKALESEL
                     GFDLFVREGRAFSRITPQGQKVLEYATRALAEIDSLKAVAADLNQDNRGTLSIATTHT
                     QARYVLPPVVQAFRERYPEVELHLHQ"
BASE COUNT          470 a          931 c          928 g          432 t
ORIGIN      
        1 tttgccgccg tgaccccgac gacctatacg ctgcaggccg tggcgactgg caaccagacc
       61 aaggacacgg ccgcctgcct gactttcacg atcaacgagc agggacagcg cacgccggcc
      121 gattcgacca actgctggcg ctgagttcgc gccgtcagtg tgaacaggcc ctagggctgc
      181 gccaggaagg cgcggatctc ggcgtcgaag cgcgggatgt cgaccaggaa cgcgtcgtgg
      241 ccctggatcg aaggcagccg cctgaagcgt gtcctcactc cgacctgctg cagcgtctcg
      301 gcgatggccg cctgctcatg gatcgggtac aggaaatcac tttcgacccc gagcaccagt
      361 gcctgctcga gaccgccgtc gcgaccgctc gcggccagcg cagcctgata tgagccgccg
      421 tgctcggcga ggtcgaatcg atccatcgcc cgcgacaggt agaggtagca gttcgggtcg
      481 tagacgcgga tgaagcgttc ggcctgcgcc tcgaggtatc cctcgacggc gaagcgcggc
      541 ccgaacgggt cggaatccag cgccagcccc gccagcggcg cacggccgaa gcgctgtcgc
      601 cattcggtgg cggatcgata ggtgatcgtg ccgagcttgc gtgccacggt catgccattg
      661 cgcggcggct tgtcgtccgc gtagtggccg ccctgccagt ccgggtcgcg catgacggcc
      721 tcgcgctgca ccgatcgcaa cgcgattgcg aacggcgagg cggcggcgga gcccgagatg
      781 ctgaccaggc ggcgcgcacc ctgcggatgg agcgcggcga aggcgaccgc tagcgagccc
      841 ccgagcgagg aacccatgac gaccgccaac cgctcgatgc cgagcgcgcg cacggcttcg
      901 aagccggcgc tggcgatgtc ctcgaccgcg atttcgggaa aatcgagccg gtagcgctca
      961 ccggtggccg gatcgatgct cgccggtccg gtcgagccga agcagctgcc gagcgaattc
     1021 acgcacacga cgaaatagcg gttggtgtcg atcgcgagcg ccggcccgat catcggctgc
     1081 caccagcccg gagtcggatc ggcggcagtc gcggcggcgt gtgccgaggg cgacagaccg
     1141 gtgaacagca ggatggcgtt gtcgcgcgcg gcgttgagcc ggccccagct ctcgtaggcg
     1201 atgcgcgcgg cacccagcca tccgccgcga taaagcggga atggatcggg cagcggcacc
     1261 gtctctggga tcacgcccgt ctcagtgggt gcgatctgcg gccggtatcg cgtggctggc
     1321 ggccgtgttt caggtttcag gatccgaccc atccgggatg ccctcgaaca gcggtgtcga
     1381 gaggtaacgc tcggccgtgt cgggcaacat cgccaggatg ctcgcgcccg cgccggcctc
     1441 gcgcgccact ttgagtgcgg cagcgaaggt ggcgccgctc gagatgccca cgaagatgcc
     1501 ttccttgctg gcgagttcgc gcgaagtcga gatcgcctcg gcgtcggtca cggtcaggat
     1561 ccggtgcgcc acttcgcggt tgagcaccgc gggcacgaaa tccggcgtcc agccctggat
     1621 cttgtgcggc gagaacggct tgcccgaaag cagcgccgcg gcctccggct cggcgacaac
     1681 gatcttcact tcgggccgcg ccaggcggat gacctcgccg gcgcctgtca aggtgccgcc
     1741 agtgccccag ccggtcacga agaaatcgag cttgcggccg acgaagtcct gcaggatctc
     1801 cgggccggtc gtgttgcggt gatacgccgg gttggccggg ttctcaaact gccgcgcgag
     1861 gaaccagccg ttcttcttgg cgagctccgc ggccttgcgc accatgccct gcccgcgctc
     1921 ggcggccggg gtcaggatca ccttcgcgcc gagcatgcgc atgatcttgc gacgctcgac
     1981 cgagaaggtc tcggccatga cgccgacgaa gggatagccc ttcgccgcgc acaccatcgc
     2041 gagcgcgatg ccggtattgc cggaagtggc ctcgaccacg gtctggcccg gcttcagcgc
     2101 tccgctgcgc tcggcgtcct cgatgatggc gatggcgagg cggtccttga ccgaagccat
     2161 cgggttgaag aactcgcact tcacgtacat cgtgacgccc tgcggcgcga gccgctggat
     2221 gcgcaccacc ggagtgcggc cgatcgtcgc cagtatgttt tcgtagatca tgccggcact
     2281 ctaatccggt gcgtcagcgc aaagcgataa gacaaaagcc attgactgat aacatgttgt
     2341 tataattagg gccacggcga cgccgggccc aggtacatga actttcatca gctccagtac
     2401 gcggtcgcga tcgcgcacca caacctttcg gtcacacagg ccgcctcggc gcttggcacc
     2461 tcgcagcccg caatcagccg cgcgctcaag gcgctcgaga gcgaactcgg cttcgacctg
     2521 ttcgtgcgcg aaggccgagc gttttcgcgc atcacgccgc agggccagaa agtgctcgag
     2581 tacgcgacac gcgcgctcgc tgagatcgac agcctcaagg ccgtggcggc cgacctgaac
     2641 caggacaacc gtggcacgct ctcgatcgcg accacgcaca cccaggcgcg ctatgtattg
     2701 ccgccggtcg tgcaggcgtt ccgcgagcgc tatcccgaag tcgaattgca cctgcaccag
     2761 g
//
LOCUS       DASRDG010000173         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_597090, whole genome shotgun sequence.
ACCESSION   DASRDG010000173 DASRDG010000000
VERSION     DASRDG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597090"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFA35_02795"
     CDS             <1..443
                     /locus_tag="VFA35_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204619.1"
                     /note="catalyzes the formation of malonyl-CoA from
                     malonate and CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HZT55125.1"
                     /translation="FKGYWRMPEKTAEEFSADGWFKTGDVGRFDARGYLTIVGRSKDL
                     IISGGFNVYPAEIEATLNEMAGVAESAVIGVPHPDFGEAVVAVVVPRPGSTPDAAALI
                     KALKSKVANFKVPKRVFVVAELPRNAMGKVQKNLLRETHTGLFA"
     gene            440..673
                     /locus_tag="VFA35_02800"
     CDS             440..673
                     /locus_tag="VFA35_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HZT55126.1"
                     /translation="MNAGPDPDRCPRCGEMFDCGANDAAPCPCTTLALDAKTLADLRA
                     RYTGCLCLRCLAELSNQPAPAASAAPPRAPGRA"
     gene            complement(612..2762)
                     /locus_tag="VFA35_02805"
     CDS             complement(612..2762)
                     /locus_tag="VFA35_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HZT55127.1"
                     /translation="MKPGARLALLVGALALALALLAAASVALVWATLNGAERDAVAGV
                     LASRAMLVAMLWLAVFGLAAWGLNWLCGRFIEAPAQLLEQAQVRLAADAAHEIEPRGS
                     AANRGLAETINTLAGQRRQLQHDVQAQVAAASRGIEQERSRLSALMAELTQSVVVCNL
                     DGRILLYNSRARDAFRAGARVTAGGAELIGLGRSIHAVFERALVAHALDTIEQHRRDG
                     AASPSAQFVTSTPGGRLLRVQMAPVRDPAGGGGLDGYVLMFDDIGREVEQEALRDRLL
                     LGLTEGNRASLGNLQAAVEMLELPDLEAPMRERFQGVVRDEVAAMTERLQAVAADVHA
                     GLKTRWPMEDMRGADLIGAALRQIEALGSVRASAADVDAGIWLRVDSYSLLRALAYLA
                     GRLVDEYEVHSLQLRLRPAGARAQLDLVWAGVAVSTETVTGWQMDAMRSGDAATPLTL
                     RDVVERHGAEFWFERERTTQSAFFRFLLPCAAAQEPHAAVAIERGDSRPEFYDFDLFQ
                     TSAQTRALDEHELARLSYTVFDTETTGLDPSGGDEIIQIGATRIVNGRLLRNECFERL
                     VDPRRELSAASTAIHGIAPEALAGQPTILEVLPVFHAFAFETVLVAHNAAFDMRFLQL
                     KEAASGLRFDHPVLDTLLLSAWVHPNQASHRLEAIAERLGVAVTGRHTALGDAMVTAE
                     VFLKLLPLLRAKGVVTLGQARAAALQTRQARVGY"
     gene            complement(2759..3133)
                     /locus_tag="VFA35_02810"
     CDS             complement(2759..3133)
                     /locus_tag="VFA35_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZT55128.1"
                     /translation="MNPKILIADDEPNIVVSLEYLMKREGYSVSIARDGEEALQAVRR
                     ERPALVLLDVMMPKKSGFEVCQEIRADAALKDTLILMLSAKGRDTDIAKGLGLGADAY
                     MTKPFSTKALVQKVRQMLEGGA"
     gene            complement(3130..>3295)
                     /locus_tag="VFA35_02815"
     CDS             complement(3130..>3295)
                     /locus_tag="VFA35_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT55129.1"
                     /translation="GDAAHRPQGTGLGLPISRQIVEHFGGRMWLRSAPGQGACFGFEL
                     PWPNPPGDSP"
BASE COUNT          487 a         1192 c         1157 g          459 t
ORIGIN      
        1 tgttcaaggg ctactggcgc atgcccgaga agaccgccga ggagttcagc gccgacgggt
       61 ggttcaagac cggcgacgtg ggccgcttcg acgcccgggg ctacctgacg atcgtcggcc
      121 gcagcaagga cctcatcatc agcggcggct tcaacgtcta ccccgccgag atcgaagcca
      181 cgctgaacga gatggccggt gtcgccgaga gcgcggtgat cggcgtgccg cacccggact
      241 tcggcgaagc ggtggtcgcg gtggtcgtgc cgcgccccgg cagcacgccc gacgcggccg
      301 cgctgatcaa ggcgctcaag tcgaaggtcg caaacttcaa ggtgcccaag cgcgtgttcg
      361 tcgtcgccga gctgccgcgc aacgcgatgg gcaaggtgca aaagaacctg ctgcgcgaaa
      421 cgcacacggg cttgttcgcg tgaacgccgg ccccgacccc gaccgctgcc cgcgttgcgg
      481 cgagatgttc gattgcggcg cgaacgacgc ggcaccctgc ccctgcacga cgctcgcgct
      541 cgacgcgaag acgctggccg atctgcgcgc gcgctacacg ggctgcctgt gcctgcgttg
      601 cctcgcggag ctcagtaacc aacccgcgcc tgccgcgtct gcagcgccgc cgcgcgcgcc
      661 tggccgagcg tgaccacgcc cttcgcgcgc agcagcggca gcagtttcag aaagacttcg
      721 gcggtcacca tcgcgtcgcc gagtgccgtg tgccggcccg tcaccgcgac gcccaggcgc
      781 tcggcaatgg cttcgagccg gtgcgacgcc tggttcgggt gaacccaggc cgagagcagc
      841 aaggtgtcga gcaccgggtg gtcgaagcgc aagccgctgg ccgcttcctt gagctgcaga
      901 aagcgcatgt cgaaggcggc gttgtgcgcg accagcacgg tctcgaacgc gaaggcatgg
      961 aacaccggca ggacctcgag gatcgtcggc tggccggcca gcgcctcggg cgcgatgccg
     1021 tggatcgccg tcgacgccgc cgagagttcg cgccgcgggt cgacgagccg ctcgaagcac
     1081 tcgttgcgca gcaggcggcc gttgacgatg cgggtcgcgc cgatctggat gatctcgtcg
     1141 ccgccggagg ggtccaggcc ggtggtctcg gtgtcgaaca cggtgtagct caaccgggcg
     1201 agttcatgct cgtcgagcgc gcgcgtctgc gccgaggtct ggaacaggtc gaagtcgtag
     1261 aactcggggc ggctgtcgcc gcgctcgatc gccaccgcgg cgtgcggctc ctgcgctgcg
     1321 gcgcaaggca gcaggaagcg gaagaacgcg ctctgggtcg tgcgctcgcg ctcgaaccag
     1381 aactcggcgc cgtggcgctc gaccacgtcg cgcagcgtca gcggcgtcgc ggcgtcgccg
     1441 ctgcgcatcg cgtccatctg ccagcccgtc acggtctcgg tgctcacggc cacgccggcc
     1501 cagaccaggt cgagctgcgc ccgcgcgccg gcggggcgca gccgcagctg caggctgtga
     1561 acttcgtact cgtcgacgag ccgccccgcc aggtaggcga gcgcgcgcag cagcgaatag
     1621 ctgtcgaccc ggagccagat gccggcatcg acgtcggccg cgctcgcgcg caccgagccc
     1681 agcgcctcga tctgccgcag tgcggcgccg atcaggtcgg cgccgcgcat gtcttccatc
     1741 ggccagcgcg tcttcaggcc ggcatgcacg tcggcggcga cggcctgcag ccgctccgtc
     1801 atggccgcga cctcgtcgcg cacgacgccc tggaaacgtt cgcgcatcgg ggcttcgagg
     1861 tccggcagtt cgagcatctc gaccgcggcc tgcaggttgc cgagcgaggc gcggttgcct
     1921 tcggtcaggc cgagcagcag gcggtcgcgc agcgcctcct gctcgacctc gcggccgatg
     1981 tcgtcgaaca tcagcacata gccgtcgagc ccgccaccgc ccgccgggtc gcgcaccggc
     2041 gccatctgca cgcgcagcaa ccggccgccc ggcgtgctgg tgacgaactg ggccgacggg
     2101 ctcgccgcac cgtcgcgccg gtgctgctcg atggtgtcga gcgcgtgcgc cacgagcgcg
     2161 cgctcgaaca ccgcgtggat cgagcgcccg aggccgatca gctcggcgcc gccggccgtc
     2221 acgcgcgcgc cagcgcggaa cgcgtcgcgg gcgcggctgt tgtagagcag gatgcggcca
     2281 tcgaggttgc acacgacgac gctctgcgtg agctcggcca tcagcgccga caagcggctg
     2341 cgctcctgct cgatgccgcg gctggcggcg gcgacttgcg cctgcacgtc gtgctgcaac
     2401 tggcggcgct ggcccgccag cgtgttgatc gtctcggcca gaccgcggtt ggcggcgctg
     2461 ccacgcggct cgatctcgtg cgcggcgtcc gcggcgagcc gcacctgggc ctgctcgagc
     2521 aactgcgccg gggcctcgat gaatcgaccg cagagccagt tcaggcccca agccgcgaga
     2581 ccgaagaccg ccagccagag catcgcgacc agcatcgcgc gcgacgcgag cacgccggcg
     2641 acggcgtcgc gctcggcacc attgagcgtc gcccagacga gcgcgacgct cgccgcggcc
     2701 aacaacgcca gcgccagcgc gagcgcgccg acgagcagcg ccaggcgcgc gccgggcttc
     2761 aagccccccc ctcgagcatc tggcgcacct tctgcaccaa cgccttggtc gaaaacggct
     2821 tggtcatgta ggcgtcggcg ccgagcccca ggcccttggc aatgtcggtg tcgcgtccct
     2881 tggcgctgag catcaggatc agcgtgtcct tgagcgccgc gtcggcgcga atctcctggc
     2941 acacctcgaa gccggacttc ttcggcatca tcacgtcgag cagcaccagc gcgggccgct
     3001 cgcgccgaac cgcttgcagc gcctcctcgc cgtcgcgggc gatgctgacg ctgtagccct
     3061 cgcgcttcat caggtattcg agcgagacca cgatgttcgg ctcgtcgtcg gcgatcagga
     3121 tcttcgggtt catggtgagt ctcccggtgg gttcggccac ggcaattcga acccaaaaca
     3181 cgcgccctgc ccgggcgccg agcgcagcca catccgaccg ccgaagtgct cgacgatctg
     3241 gcggctgatc ggcaggccga ggccggtgcc ctgcgggcgg tgcgcggcat cgccg
//
LOCUS       DASRDG010000174         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1410770, whole genome shotgun sequence.
ACCESSION   DASRDG010000174 DASRDG010000000
VERSION     DASRDG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410770"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFA35_02820"
     CDS             <1..600
                     /locus_tag="VFA35_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HZT55130.1"
                     /translation="VLLALLNRAKVTSIGPYVVVGLLVWVCVLKSGIHATLAGVVTAM
                     AIPLADGKGGAPLETAEHALHPWVAFVVLPVFAFANAGVSLAGVSLGTLAQGVPLGIA
                     VGLVAGKAIGVFGAAWLLMRATGAGLPAGSTTRQFFGVCVLCGIGFTMSLFIGGLAFA
                     GQNPAYETQVKLGVLGGSLIAGLLGVALLLSGPRPASPR"
     gene            complement(569..1192)
                     /locus_tag="VFA35_02825"
     CDS             complement(569..1192)
                     /locus_tag="VFA35_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HZT55131.1"
                     /translation="MAQAPDPHAPKPAVKLRPLPAFIFMSRWLQLPLYLGLILAQCVY
                     VFHFWVELVHLVEAAFGNRAALTALTEGMGYVFPDGSPGPKLTETVIMLAVLALIDVV
                     MISNLLIMVIVGGYETFVSRMNLEGHPDEPEWLSHVNASVLKVKLGTAIIGISSIHLL
                     KSFISAGDMKPEVLMWQTLIHITFLLSAIAIAYTDKLLNAAKPAEAH"
     gene            1339..>2208
                     /locus_tag="VFA35_02830"
     CDS             1339..>2208
                     /locus_tag="VFA35_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZT55132.1"
                     /translation="MSATETHVYNPSAAAMKDACVSGMDAYRALVAEAEADHEAFWAR
                     QARELLSWKTPFTKVLDTSDAPFFKWFEDGTLNASYNCLDRQVEAGLGDKVALIFEAD
                     DGKVTKVTYRELLTRTCQLANALRARGVKKGDRVAIYLAMSIEGVVAMQACARIGATH
                     TVIFGGFSAQSLRDRIADTGAVLVITADEQLRGGKQLPLKAIVDEALALEGCESVKDV
                     IVFKRTGGKIAWNDARDHWLHDEVKNQPTTCEPEWVEAEHPLFLLYTSGSTGAPKGVQ
                     HATGGYLLHAALTC"
BASE COUNT          364 a          724 c          772 g          348 t
ORIGIN      
        1 gtgctgctgg cgctcctgaa ccgcgcgaaa gtgacgagca tcgggcctta cgtcgtggtc
       61 ggcctgctgg tctgggtctg cgtgctgaag tcgggcatcc atgcgacgct cgccggggtc
      121 gtcaccgcga tggcgatccc gctcgccgac ggcaagggcg gcgcgccgct cgagaccgcc
      181 gagcacgcgc tgcacccgtg ggtcgcgttc gtcgtgctgc cggtgttcgc gttcgcgaac
      241 gcgggcgtga gcctggccgg cgtgtcgctc ggcacgctgg cgcagggcgt gccgctgggc
      301 atcgcggttg gcctggtggc cggcaaggcg atcggtgtgt tcggcgccgc gtggctgctg
      361 atgcgcgcga ccggcgccgg gctgccggcc gggtcgacga cgcgccagtt cttcggtgtg
      421 tgcgtgctgt gcgggatcgg cttcacgatg agcctgttca tcggcgggct cgcgttcgcc
      481 gggcagaacc cggcctacga gacccaggtc aagctcggcg tgctcggcgg ttcgctgatc
      541 gccgggctgc tcggcgtggc gctgctgctc agtgggcctc ggccggcttc gccgcgttga
      601 gcagcttgtc ggtgtaggcg atcgcgatcg ccgacagcag gaaggtgatg tggatcaggg
      661 tctgccacat cagcacctcg ggcttcatgt cgccggcgct gatgaagctc ttcagcaggt
      721 ggatcgagct gatgccgatg atcgcggtgc cgagcttgac cttcagcacc gaggcgttca
      781 catggctcag ccactcgggc tcgtccggat ggccttccag gttcatgcgg ctgacgaagg
      841 tctcgtagcc gccgacgatc accatgatca gcaggttcga gatcatcacg acgtcgatca
      901 gcgccagcac ggccagcatg atcacggtct cggtcagctt cgggccgggg ctgccgtccg
      961 ggaacacata gcccatgccc tcggtcagcg ccgtgagcgc ggcccggttg ccgaacgccg
     1021 cctcgaccag atggacgagc tcgacccaga aatgaaacac gtagacgcat tgcgccagga
     1081 tcagcccgag gtacagcggc agctgcagcc agcggctcat gaagatgaac gccggcaacg
     1141 ggcgcaactt gaccgcgggt ttcggagcgt gaggatcggg agcttgggcc atgcgcagag
     1201 gagcagacga gagagatact gcggaaaatt gtagcggccc ggagttcgcc gctagagtaa
     1261 gccgttcgta agagcaaatg cctaccctgc cagccaaagc agcagtctgg ggccgacccc
     1321 aacacttgga gacaaaccat gagcgcaacc gagacccacg tgtacaaccc gtccgccgcg
     1381 gccatgaagg acgcttgcgt gtccggcatg gatgcctacc gtgcactcgt cgccgaggcc
     1441 gaggccgacc acgaggcctt ctgggcgcgc caggcgcgcg agctgctcag ctggaagacg
     1501 ccgttcacca aggtgctcga caccagcgac gcgccgttct tcaagtggtt cgaggacggc
     1561 accctgaacg cctcgtacaa ctgcctggac cgccaggtcg aggcgggcct gggtgacaag
     1621 gtcgcgctga tcttcgaggc cgacgacggc aaggtcacga aggtcaccta ccgcgaactg
     1681 ctgacccgca cctgccagct cgcgaacgct ttgcgcgcgc gtggcgtcaa gaagggcgac
     1741 cgggtcgcga tctacctcgc catgtcgatc gagggcgtgg tcgcgatgca ggcgtgcgcg
     1801 cgcatcggcg cgacgcacac ggtgatcttc ggcggcttct cggcgcagtc cctgcgcgac
     1861 cgcatcgccg acaccggcgc ggtgctggtg atcaccgccg acgaacagct gcgcggcggc
     1921 aagcagctgc cgctgaaggc gatcgtcgac gaggcgctcg cgctcgaagg ctgcgagtcg
     1981 gtgaaggacg tgatcgtgtt caagcgcacc ggcggcaaga tcgcctggaa cgacgcgcgc
     2041 gaccactggc tgcacgacga ggtcaagaac cagccgacga cctgcgagcc cgagtgggtg
     2101 gaggcggagc acccgctctt cctgctctac acctccggct ccaccggcgc ccccaagggc
     2161 gtgcagcacg ccaccggcgg ctacctgctc cacgcggcgc tgacctgc
//
LOCUS       DASRDG010000175         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_141351, whole genome shotgun sequence.
ACCESSION   DASRDG010000175 DASRDG010000000
VERSION     DASRDG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141351"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFA35_02835"
     CDS             <1..186
                     /locus_tag="VFA35_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55133.1"
                     /translation="GAITGTAESDLFMMNSPKGVMPRLAMSRRVGLQRRLNVRDALTA
                     RHPAPRGTRRVDAAAVV"
     gene            complement(226..1029)
                     /locus_tag="VFA35_02840"
     CDS             complement(226..1029)
                     /locus_tag="VFA35_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55134.1"
                     /translation="MKYLRLWQLGLLVLIFVLWHVLTRPGLIPPFVFENDQQAAFFFG
                     EPLKVFGRIWNWFVVNADIYEHLWVTLLETVLAFGIGTVLGLACGLWLALSPMAAAIT
                     DPYIKALNSMPRVILAPIFAVWFGLGLASKVALGVTLVFFIVFFNVYQGVKEVSPVVL
                     ANARMLGASRRQLLRHVYLPSATSWVFSSLHTSVGLAFVGAVVGEYLGSSRGVGYLIL
                     QAEGSFDIDTVMAGILVLTVFALALDAAVGRIERRLMKWQPQASDAERL"
     gene            complement(1026..1838)
                     /locus_tag="VFA35_02845"
     CDS             complement(1026..1838)
                     /locus_tag="VFA35_02845"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT55135.1"
                     /translation="MPPDAASALSLSGVSCTFTDKDNPSRRYTAVRDVSLSVGAGEFV
                     SVVGPTGCGKSTLLNVAAGLLAPSAGEVTVFGEPLVGINRRAGYMFQAESLMPWRTAA
                     QNVVAGLEFRGAPDARAQADEWLKRVGLGAFGDRYPHQLSGGMRKRVSLAQTLVLDPD
                     IILMDEPFSALDVQTRQLMENEVLELWSSKRKAVLFITHDLDEAIAMSDRVVCLSAGP
                     GARPIGEFVIDLQRPRDVAEVRVTPRFVELHQAIWGVLRDEVLKGYRQQLAA"
     gene            2151..>2794
                     /locus_tag="VFA35_02850"
     CDS             2151..>2794
                     /locus_tag="VFA35_02850"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZT55136.1"
                     /translation="MPQFVRFAPQATPPATALAALRTLPVSTVLAASLLVALLLSTEY
                     LFQPFVWRHWPVDEVLLGWLEVLRDRVGVAATIGVALAVAAHLPVRSLAARSVALALA
                     IVAGAAAGELLLVAAGARGASDDAVAVFGRIARWAVIACSIGAMWFVWRRGAEASARA
                     QALELRRVRLEREATEAQLGALRGQIDPHFLFNTLATVRRLRQVEPARGARLLA"
BASE COUNT          435 a          980 c          966 g          413 t
ORIGIN      
        1 ggtgccatca ccgggactgc tgagagtgac cttttcatga tgaattctcc gaagggtgtg
       61 atgccgagat tggcgatgtc gcggcgtgta gggttgcagc gacgcctcaa tgtgcgcgac
      121 gcgttgacgg ctcgtcatcc cgcgccccgg gggactcggc gcgtcgatgc agcggcagtc
      181 gtgtgaagct ccacaccagg cgatgcagag cactcgaatc gggggtcaga gtctttcggc
      241 gtcactcgcc tgcggctgcc acttcatcag acgcctctcg atgcgcccga ctgcggcgtc
      301 gagcgccagc gcgaacaccg tcagcaccag gatgcccgcc atcacggtgt cgatgtcgaa
      361 cgagccctcg gcctgcaaga tcaggtagcc cacgccgcgt gacgagccga ggtattcgcc
      421 gaccaccgcg ccgacgaacg cgaggccgac gctggtgtgc agcgagctga acacccagct
      481 cgtcgcgctc ggcagataca cgtgccgcag caattgccga cgcgacgcgc cgagcatgcg
      541 cgcgttcgcg agcaccaccg gactcacctc cttgacgccc tggtagacgt tgaagaagac
      601 gatgaagaac accagcgtca caccgagcgc aaccttgctc gcgagcccca agccgaacca
      661 gaccgcgaag atcggcgcga ggatcacgcg cggcatgctg ttcagcgcct tgatgtacgg
      721 gtcggtgatg gccgcggcca tcgggctcag cgccagccac agcccacaag ccaggccgag
      781 cacggtgccg atgccgaacg cgagcacggt ctcgagcagc gtgacccaca agtgctcgta
      841 gatgtcggcg ttgacgacga accagttcca gatgcggccg aagaccttca gcggctcgcc
      901 gaagaagaac gcggcctgct ggtcgttctc gaacacgaac ggtgggatca gaccgggccg
      961 ggtcagcaca tgccacagca cgaagatcag caccagcagg ccgagctgcc acaggcgcag
     1021 gtacttcatg cggcgagctg ctgccggtag cctttcagca cctcgtcgcg cagcacgccc
     1081 cagatggcct gatgcagctc gacgaagcgc ggggtcacgc gcacctcggc cacgtcgcgc
     1141 gggcgctgca gatcgatcac gaattcgccg atcgggcgtg cgcccggccc ggcggaaagg
     1201 cacacgacgc ggtcgctcat cgcgatcgcc tcgtcgagat cgtgcgtgat gaacagcacc
     1261 gcttttcgct tggacgacca cagctcgagc acttcgtttt ccatcagctg ccgcgtctgg
     1321 acgtcgagcg ccgagaacgg ctcgtccatc aggatgatgt cggggtcgag cacgagcgtc
     1381 tgcgccaggc tcacgcgctt gcgcatgccg cccgagagct ggtgcggata gcggtcgcca
     1441 aaagcgccga ggccgacacg cttcagccac tcgtcggcct gcgcgcgtgc gtcgggcgcg
     1501 ccacgaaatt cgagccccgc caccacgttc tgcgccgcgg tgcgccacgg catcaggctc
     1561 tcggcctgga acatgtagcc ggcgcggcga ttgatgccca cgagcggctc accgaacacc
     1621 gtgacctcgc cggcgctcgg cgcgagcagg ccggcggcga cgttgagcaa ggtgctcttg
     1681 ccgcagccgg tcgggccgac gaccgagacg aactcgccgg cgccgacgct caagctcacg
     1741 tcgcgcaccg cggtgtagcg ccggctcggg ttgtctttgt cggtgaacgt acaactgacg
     1801 cctgaaaggc tcagcgcgga tgcagcatcg ggcggcatgg ggggactcga ggccaacggt
     1861 gaagacggcc gactgtacca atcgacgtgg catcgcttgc gccgcctggt tcgtcgcccc
     1921 ctggcggcat tcgtcgcggc cagggccgcg ttcgtcgcat cgcgcccgcg accgggcggc
     1981 cacggtttac ggtgacctcg ccgacacgcg catcacgcag gggcaccgca cccatgaagc
     2041 ttcgaattcc gctggccccg cctgcggcgc ccggctacga tgcggcgacg ccccggccca
     2101 cgcgcagctg cgtgccgaca cggtggcgcc gcgctcgggg aggcgattcg atgccgcagt
     2161 tcgtgcgatt cgcgccgcag gccacgccgc ctgcgaccgc cctggccgcc ctgcgcacgc
     2221 tgccggtgtc gaccgtgctc gcggcgagcc tgctggtcgc gctgctgctg tcgaccgaat
     2281 acctgttcca gcccttcgtc tggcgccact ggccggtcga cgaggtcttg ctcggctggc
     2341 tcgaggtgct gcgcgaccgc gtcggcgtcg ccgcgacgat cggcgtcgca ctcgccgtgg
     2401 cggcgcacct gccggtgcgc tcgctggccg cgcgctcggt ggcgctggcg ctggcgatcg
     2461 tcgccggcgc cgcggccggc gagctgctgc tggtcgcagc cggcgcgcgc ggcgcctccg
     2521 acgatgcggt ggcggtgttc ggccgcatcg cgcgctgggc cgtcatcgcg tgcagcatcg
     2581 gcgcgatgtg gttcgtgtgg cggcgcggtg ccgaagcgag cgcccgggcc caggcgctcg
     2641 agctgcggcg cgtgcggctc gagcgcgagg ccaccgaagc gcagctcggg gcgctgcgcg
     2701 gccagatcga tccgcacttt ctgttcaaca cgctggccac cgtgcgccgg ctgcgccaag
     2761 tcgagccggc gcggggcgcg cggctgctgg cgca
//
LOCUS       DASRDG010000176         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_829841, whole genome shotgun sequence.
ACCESSION   DASRDG010000176 DASRDG010000000
VERSION     DASRDG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829841"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..>2275
                     /locus_tag="VFA35_02855"
     CDS             31..>2275
                     /locus_tag="VFA35_02855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZT55137.1"
                     /translation="MKLALSAPLPLWTIPTAQFAAGLLRALQQQGPVHALVENPFRVQ
                     PGLAAGDLLQPLCELPELQRTGAVDVPLHLVGDDPLHLHQLRYLRMCPGVLVLPSLAL
                     GRLRDCLWTEVGGRRVPFVKGFDAHQDLAVVLAGMSRAVAVSSEADARALRASLPQAA
                     LPQAAVHVVPGLAPSQPDFAAAAARLREIAEKLPAGPAFVAEDPAQWPPVTAVVIGYN
                     SRDIVGPCLQTLLDQDYPNLEVVVVDNASADGTADFVRTKFPSIKLIASDENLGFAEG
                     NNLVFRTARSQYVVLLNQDAYARRNWVTELVRAAEIDERIASVGSKMLFYRCPTLLNS
                     TAIEINQAGWGWDRQVGEKDENPSPMPEAVFGGCGGAVLYRRSALEKVGGFDRAFFMY
                     YEDTDLAWRFRIAGYENLYAPLAVVRHDFHGDTGATPGRLFRRRFMSERNRWATLFKN
                     LDWGTMLRLLPKIRRHDKGRIAWLERAVRENHNPEFHGQIARIVKQAWRYNVLRLPQL
                     LLRRRRTQRLRAVPPAVSNRFLVAGINEGGHQGDVDNFHDRFSCQRTDAIRMGENDVG
                     TIGSGWHVLENPPGALRPYRWCKGRAWFYLQPKASTQALFLRLQSPMEPHRVEVFAES
                     ERLGSCTVDASGGEYCFPLPASLPRGEVLEFTLVSATLQPKAAGLSGDVRDLGVTVSE
                     MRCADAVPAGLPAGEHWAGTGFDRAQAPRRAVVRMGESDDGVLGGGFYGVERPPGAVQ
                     PYRWCGQR"
BASE COUNT          317 a          827 c          807 g          324 t
ORIGIN      
        1 ctggcaagct ggccgaagag actctgaccg atgaagctcg ccctctctgc gccgctgccg
       61 ctgtggacga tcccgacggc gcagttcgcg gcggggctgc tgcgcgcgct gcagcagcag
      121 gggccggtgc acgcgctggt cgagaacccc ttccgcgtgc agccggggct cgccgcgggc
      181 gacctgctgc agccgctgtg cgagctgccc gagctgcagc gcaccggcgc cgtcgacgtg
      241 ccgctccatc tcgtcggcga cgacccgctg cacctgcatc agctgcgcta cctgcgcatg
      301 tgccccggcg tgctcgtgct gccgtcgctc gcgctcggca ggctgcgcga ttgcctgtgg
      361 accgaggtcg gcggcaggcg cgtgccgttc gtcaagggct tcgacgcgca ccaggacctc
      421 gcggtcgtgc tcgcgggcat gagtcgcgcc gtcgcggtga gcagcgaagc cgacgcgcgc
      481 gcgctgcgcg cctcactgcc gcaggccgcg ctgccgcagg ccgcggtgca cgtcgtgccc
      541 gggctcgcgc catcgcagcc agacttcgcc gccgccgcag cgaggctgcg cgagatcgcc
      601 gaaaagctgc ccgcgggccc cgcgttcgtc gccgaggatc cggcgcagtg gccgccggtg
      661 accgcggtgg tgatcggcta caacagcagg gacatcgtcg gcccgtgcct gcagacgctg
      721 ctcgaccagg actacccgaa cctcgaggtg gtcgtcgtcg acaacgcgag cgcggacggc
      781 accgccgact tcgtgcgcac gaagttcccg tcgatcaagc tgatcgcgag cgacgagaac
      841 ctcggtttcg ccgagggcaa caacctggtg ttccgcaccg cgcgctcgca gtacgtcgtg
      901 ctgctgaacc aggacgccta tgcgcgccgc aactgggtga ccgagctcgt gcgcgcggcg
      961 gagatcgacg aacgcatcgc gtccgtcggc agcaagatgc tcttctatcg ctgcccgacg
     1021 ctgctcaact cgacggcgat cgagatcaac caggccggct ggggctggga ccgccaggtc
     1081 ggcgagaagg acgagaaccc ttcgccgatg ccggaggcgg tgttcggcgg ctgcggcggc
     1141 gcggtgctct accgcaggag cgcgctcgag aaggtcggcg gcttcgaccg cgcgttcttc
     1201 atgtattacg aggacacgga tctggcgtgg cgcttccgca tcgccggcta cgagaacctc
     1261 tatgcgccgc tcgcggtcgt ccgccacgac ttccacggcg acaccggcgc cacgccgggc
     1321 cggttgttcc gccgccgctt catgagcgag cgcaaccggt gggcgacgct gttcaagaac
     1381 ctcgactggg gcacgatgct gcgcctgctg ccgaagatcc gccgccatga caagggccgc
     1441 atcgcgtggc tcgagcgcgc cgtgcgcgag aaccacaatc ccgagttcca cggccagatc
     1501 gccaggatcg tcaaacaggc gtggcgctac aacgtgctgc gcctgccgca gctcctcctg
     1561 cgccgccgcc gcacgcagcg cctgcgcgcc gtgccgcccg ccgtcagcaa ccgcttcctc
     1621 gtcgccggca tcaacgaagg cggtcaccag ggggacgtcg acaacttcca cgaccgtttc
     1681 agctgccagc gcaccgatgc gatccgcatg ggcgagaacg acgtcggcac catcggctcg
     1741 ggctggcacg tgctcgagaa cccgcctggc gcgctgcgtc cgtaccgctg gtgcaagggc
     1801 cgcgcgtggt tctacctgca gccgaaggcg tcgacgcagg cgctgttcct gcgcctgcag
     1861 agcccgatgg aaccgcaccg cgtcgaggtg ttcgcggaga gcgagcgcct cggctcatgt
     1921 accgtcgacg cgtccggcgg cgagtactgc tttccgctgc cggcgtcgct gccgcgcggc
     1981 gaggtgctcg agttcacgct ggtcagcgcg acgctgcagc cgaaggccgc gggcctgagc
     2041 ggcgacgtgc gcgacctcgg cgtcaccgtc agcgagatgc gctgcgccga cgcggtgccc
     2101 gcgggcctgc ccgccggcga gcactgggcc ggcaccggct tcgaccgcgc gcaggcgccg
     2161 cgccgcgccg tcgtgcgcat gggcgagagc gacgacggcg tgctcggcgg cggcttctac
     2221 ggcgtcgagc ggccgcctgg cgcggtgcag ccgtaccgct ggtgcggcca gcgcg
//
LOCUS       DASRDG010000177         4275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1058822, whole genome shotgun sequence.
ACCESSION   DASRDG010000177 DASRDG010000000
VERSION     DASRDG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058822"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VFA35_02860"
     CDS             complement(<1..826)
                     /locus_tag="VFA35_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55138.1"
                     /translation="MDETGAPAPATKTRLWRRHPVGFVAATLVGLVLALLIAAAAGAW
                     WALRSERGSAWLLAALPGVQVEAPQGRLLGDFAARRVTIHLPGSADTVTLTGLAWRGL
                     RVQRAAAPQWASVTLDALAAERVDLQLGPSKNTGPMQAPADLALPIALDLRALRIGEL
                     HANALGAQPIRELVAQLQLGANQGAEHRIGAFSLGWDRLQISGAAQIASAAPMALAAQ
                     VALRQQAEGALPGWSANAALTGPLAAPVLQATLRAAPSPQHPAQALDARATLRPFAA"
     gene            complement(826..2697)
                     /locus_tag="VFA35_02865"
     CDS             complement(826..2697)
                     /locus_tag="VFA35_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZT55139.1"
                     /translation="MTLGRAACAAMVVALVGFCAGCASLGKRDAEAAAPAGAASAPAT
                     RAEYRLEVQAPDPLRALLLSYLDLARFQNAPATDSVDAIELERLLRAAPAQARGLLET
                     EGYFNAEVSAARAGTQDGLPLLRITVQPGPRVQVREASIGVVGELQASAAAGDAAAQR
                     ELAALRAQWPLRAGAAFRQAAWSDAKNTTLAKLRADGYAAAAWRSTSARVDAPANRAE
                     LALELDSGPLYKLGAIQIEGVQRYDEDAIRKLSTFGPGDAYNEKLLLDFQERLQKLGL
                     FEGASVELDADPRTANAAPVRVRVKELTQQQATVGIGYSANTGPRLSIEHTNRRVFGT
                     RWIAKNKLELGPSNKTWQGELNSYPLDGLYRNLVSGSATQLHADDQQLIGWNARIGRT
                     QDTPRIERLYFGELAHARVDTAQLSSQGDAVSANYHWIFRDIDNVLLPTKGLTTSAQA
                     GLGYARGSRSVLGDPVETAKGPFARAYARLTWYQPFGNGWFGTARAEAGQVFTHSAVG
                     IPDTLLFRAGGDESVRGYGYRTLGPEINGVTVGGRTLLTGSVEIARPISPKYPAYWWA
                     AFVDAGNAADQWKDLKPALGYGVGLRWRSPVGPLRVDLAYGQQVHRVRVHVSVGIAF"
     gene            complement(2694..3890)
                     /locus_tag="VFA35_02870"
     CDS             complement(2694..3890)
                     /locus_tag="VFA35_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZT55140.1"
                     /translation="MLIDFFLTLRAAKLKVSVKEYLMLLEAIKSGVLDADASDASGPT
                     VDKFYYLARSALVKDEASFDKFDRAFAAYFKGVEMLTDFTHELPLDWLRKKLELELSA
                     EEKAKIEAMGWDELMATLKKRFEEQKKRHEGGSKMIGTGGTSPFGAYGYNPQGIRIGQ
                     DKSRNKSAVKVWDQRQFKDYDDSLELGTRNIKVALRRLRRFAREGSEEELDLDDTIRS
                     TAANAGWLDIKMVPERHNKVKVLLLMDVGGTMDEHIHRVEEIFSAAKSEFKHLEFYYF
                     HNCVYDFMWKNNRRRYSEKFSTWDVIRKYNKDYKLIFVGDATMSPYEILQPGGSVEYN
                     NEEPGAEWLARLTQAFPKFAWINPEPQGVWGYRQSIAVVQQLMNQRMFPLTLAGLEGA
                     MRLLSK"
     gene            3982..>4275
                     /locus_tag="VFA35_02875"
     CDS             3982..>4275
                     /locus_tag="VFA35_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55141.1"
                     /translation="MLSSLTPTDVAFVMAGLMQAVPAVLWLIGGWALGRSSGTERAAV
                     HWSAYAAASALSFVFLVAAMQSRGDPQRAEWLRAVGNICGVIGLMALQRGIWLF"
BASE COUNT          526 a         1510 c         1551 g          688 t
ORIGIN      
        1 aggccgcgaa cgggcgcagc gtcgcgcgcg cgtcgagcgc ctgcgccggg tgctgcggcg
       61 acggcgcggc gcgcagcgtc gcctgcagca ccggcgccgc gagcgggccc gtgagcgccg
      121 cgttcgcgga ccagccgggc agcgcgcctt cggcctgctg gcgcagcgcg acctgcgcgg
      181 ccagcgccat cggcgcggcg ctcgcgatct gcgccgcgcc gctgatctgg agccggtccc
      241 agcccagcga gaacgcgccg atgcggtgct cggcgccctg attcgcgccg agctgcaatt
      301 gcgcgacgag ttcgcggatc ggctgcgcgc cgagcgcgtt cgcgtgcagc tcgccgatgc
      361 gcagcgcgcg caggtcgagc gcgatcggca gcgccaggtc ggccggtgcc tgcatcggcc
      421 cggtgttctt gctcgggccg agctgcaggt cgacgcgctc ggcggccagc gcatcgagcg
      481 tcacgctcgc ccactgcggc gcggcggcgc gctgcacgcg caggccgcgc cacgcgaggc
      541 cggtcagcgt gaccgtgtcg gcgctgcccg gcaggtggat cgtcacgcgt cgcgccgcga
      601 agtcgccgag cagccgccct tgcggcgcct ccacctgcac gcccggcagc gccgccagca
      661 gccacgcgct gccgcgctcg ctgcgcagcg cccaccacgc gccggccgcg gcggcgatca
      721 gcagtgcgag caccaggccg acgagcgtgg ccgcgacgaa ccccacgggg tggcgccgcc
      781 acagccttgt cttggtggcg ggcgcgggcg cgccggtctc gtccatcaga acgcgatccc
      841 cacgctgacg tgcacgcgca cccgatgcac ctgctggcca tacgccaggt cgacccgcag
      901 cggcccgacc gggctgcgcc agcgcaggcc gacgccgtag ccgagtgcgg gcttcaggtc
      961 cttccactgg tcggccgcgt tgccggcgtc gacgaacgcg gcccaccagt aggccgggta
     1021 cttcggcgag atcggccgcg cgatctcgac gctgccggtc agcagcgtgc ggccgccgac
     1081 ggtcacgccg ttgatctcgg ggccgagcgt gcggtagccg tagccgcgca ccgactcgtc
     1141 gccaccggcg cggaacagca aggtgtcggg gatgccgacg gcgctgtgcg tgaacacctg
     1201 gccggcctcg gcgcgcgcgg tgccgaacca gccgttgccg aagggctggt accaggtcag
     1261 ccgcgcatag gcccgcgcga acggcccctt cgcggtctcg accgggtcgc cgagcaccga
     1321 gcggctgccg cgcgcgtagc cgagcccggc ctgcgccgag gtcgtcagcc ccttggtcgg
     1381 cagcagcacg ttgtcgatgt cgcgaaagat ccagtggtag ttggccgaga cggcgtcgcc
     1441 ctggctcgac agctgcgcgg tgtcgacccg cgcgtgcgcg agttcgccga agtacaggcg
     1501 ctcgatgcgc ggcgtgtcct gggtgcggcc gatgcgcgcg ttccagccga tcagctgctg
     1561 gtcgtcggca tgcagctggg tggcgctgcc ggagacgagg ttgcggtaca gcccgtcgag
     1621 cgggtacgaa ttgagctcgc cctgccaggt cttgttgctg gggccgagct cgagcttgtt
     1681 cttcgcgatc cagcgcgtgc cgaacacgcg ccggttggtg tgctcgatcg acagccgcgg
     1741 cccggtgttc gcgctgtagc cgatgcccac ggtggcctgc tgctgcgtca gctccttcac
     1801 gcgcacccgc accggcgcgg cattggcggt ccgcgggtcg gcgtcgagct cgaccgaggc
     1861 gccttcgaac aggccgagtt tctgcagccg ctcctggaag tcgagcagca gcttctcgtt
     1921 gtacgcgtcg ccggggccga aggtcgagag cttgcggatc gcgtcctcgt cgtagcgctg
     1981 cacaccctcg atctggattg cgccgagctt gtacagcggg ccgctgtcga gctccagcgc
     2041 gagctcggcg cggttggccg gcgcgtcgac gcgcgcgctg gtgctgcgcc aggccgcggc
     2101 ggcgtagccg tcggcgcgca gcttcgcgag cgtggtgttc ttcgcgtccg accaggccgc
     2161 ctggcggaac gccgcgcccg cgcgcagcgg ccactgcgcc cgcagcgcgg cgagctcgcg
     2221 ctgcgcggcg gcgtcgccgg cggccgccga ggcctgcagc tcgccgacga cgccgatgct
     2281 cgcctcgcgg acctgcacgc gcgggccggg ctgcacggtg atgcgcagca gcggcaggcc
     2341 gtcctgcgtg ccggcgcgcg cggcgctcac ctcggcgttg aagtagccct cggtctcgag
     2401 cagcccgcgc gcctgcgccg gcgcggcgcg cagcaggcgc tcgagctcga tcgcgtcgac
     2461 gctgtcggtg gcgggcgcat tctggaagcg cgcgaggtcg aggtagctca gcagcagcgc
     2521 gcgcagcgga tcgggtgcct gcacctcgag ccggtactcg gcgcgggtcg cgggtgccga
     2581 ggcggcgccg gccggcgcgg cggcctcggc atcgcgcttg cccaggctgg cgcagcccgc
     2641 acagaacccg accagcgcca cgaccatcgc ggcgcacgcc gcgcggccca gggtcatttg
     2701 ctcagcagcc gcatggcccc ttccagcccg gcgagcgtga gcgggaacat gcgctggttc
     2761 atcaactgct ggaccaccgc gatgctttgc cggtagcccc atacgccttg cggctcgggg
     2821 ttgatccagg cgaacttcgg gaaggcctgc gtgagccgcg cgagccactc ggcgcccggc
     2881 tcctcgttgt tgtattcgac gctgccgccg ggctgcagga tctcgtacgg gctcatcgtc
     2941 gcgtcgccga cgaagatcag cttgtagtcc ttgttgtact tgcggatcac gtcccaggtg
     3001 ctgaacttct cgctgtagcg gcgccggttg ttcttccaca tgaagtcgta gacgcagttg
     3061 tggaagtaat agaactcgag gtgcttgaac tcgctcttcg cggccgagaa gatctcctcg
     3121 acgcggtgga tgtgctcgtc catcgtgccg cccacgtcca tcagcagcag caccttcacc
     3181 ttgttgtggc gctcgggcac catcttgatg tcgagccagc cggcgttggc cgcggtgctg
     3241 cggatggtgt cgtcgaggtc gagctcttct tccgagcctt cgcgcgcgaa tcggcgcagg
     3301 cggcgcagcg ccaccttgat gttgcgcgtg ccgagttcga gcgagtcgtc gtagtccttg
     3361 aactggcgct ggtcccagac cttcaccgcg ctcttgttgc gcgatttgtc ctggccgatg
     3421 cggatcccct gcgggttgta gccgtaggcg ccgaacgggc tggtgccgcc ggtaccgatc
     3481 atcttgctgc cgccttcgtg gcgcttcttc tgctcttcga agcgcttctt gagcgtggcc
     3541 atcagctcgt cccagcccat cgcctcgatc ttggctttct cttcggcgct gagttcgagt
     3601 tcgagcttct tgcgcagcca gtcgagcggc aactcgtgcg tgaagtcggt cagcatctcg
     3661 acgcccttga agtaggcggc gaacgcgcgg tcgaacttgt cgaagctggc ctcgtccttc
     3721 accagcgcgc tgcgcgcgag gtagtagaac ttgtcgacgg tcgggccgct cgcgtcggac
     3781 gcgtcggcgt cgagcacgcc gctcttgatc gcctcgagca gcatcaggta ctccttgacc
     3841 gagaccttca gcttggcggc tcgcagcgtg aggaagaagt cgatcagcat gggcgccagc
     3901 atacccgctg gcgcatcgcc gggtgtcggt gggcgccgcg gtagcgggca tacttccagc
     3961 gagcccgccg ggagccttcg gatgctgtcg agcctgaccc ccaccgacgt cgccttcgtg
     4021 atggccggcc tgatgcaggc ggtgcccgcg gtgctgtggc tgatcggcgg ctgggcgctg
     4081 ggccgcagca gcggcaccga gcgcgccgcg gtgcattggt cggcatacgc ggccgccagc
     4141 gcgctgtcgt tcgtgttcct ggtcgcggcg atgcagtcgc gcggcgatcc gcagcgcgcc
     4201 gagtggctgc gcgccgtcgg caacatctgc ggcgtgatcg ggctgatggc gctgcagcgc
     4261 ggcatctggc tgttc
//
LOCUS       DASRDG010000178         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1058834, whole genome shotgun sequence.
ACCESSION   DASRDG010000178 DASRDG010000000
VERSION     DASRDG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058834"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFA35_02880"
     CDS             <1..346
                     /locus_tag="VFA35_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55142.1"
                     /translation="RHSGAGAAGLVGHGQALAVRSLDWMPGWAMFAVVVLPWFVAAEW
                     AHPGFLHYFFVVQQLQRFASCGFTNAQPFWFYAPVLFLFTLPWSGWWLANLRKSTAAR
                     SSRATAVSPMCV"
     gene            complement(397..555)
                     /locus_tag="VFA35_02885"
     CDS             complement(397..555)
                     /locus_tag="VFA35_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55143.1"
                     /translation="MRERNTHDPGNAADGSKGRAVRHSVAALRDRSISGCAVSTTGSA
                     SGGSARST"
     gene            594..1016
                     /locus_tag="VFA35_02890"
     CDS             594..1016
                     /locus_tag="VFA35_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55144.1"
                     /translation="MVHGGVAFVDEYRYDVPFHLRDTSPVMVVSNWDPAEVRQHDNWR
                     KEMADAAGFAPTAGADRLLDENEFEAALCTGRIHWVIAGLDSAKHHHFLEVAQRIATD
                     RDSALWQVDAARPALAAAAGCAGAPLAKPDSPGRPRSR"
     gene            complement(1019..1519)
                     /locus_tag="VFA35_02895"
     CDS             complement(1019..1519)
                     /locus_tag="VFA35_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HZT55145.1"
                     /translation="MSRSSGATPRHSHHRLVRWHRIALYASGVALLLSGGIWLAVHYS
                     VGAGAGELPHPLEAWCMRVHGLAAFCGLFTLGALAATHIPQGWRLVHRRRWSGQRRTG
                     LMLCAAGAALALTGYALYYFAPEGVRPALGWAHAVVGVSMACLLQHHRRGQRERDEAA
                     ARPEPR"
     gene            complement(1524..2276)
                     /locus_tag="VFA35_02900"
     CDS             complement(1524..2276)
                     /locus_tag="VFA35_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZT55146.1"
                     /translation="MIAAPAAHGWRRRAQPLLGTLVEIGIVDGRADKHASDAGFAVIA
                     AVQACLSRYERASDIARFNGLRRGESLAMRPLTAEVLKAASQLERLTDGLFDISLGSA
                     PGGWRIEGNRLVKLAGCTRLDLGGIGKGFAIDHAVQALVAAGVGSGWVNAGGDLRAFG
                     DAELPIGLRDERRGGVRHFATLRDGAFATSHFGRETRSSLAHRGVRSVQAHVSVAAPC
                     CLWADALTKVVAASGDTAHPVLAHFGASAWQH"
     gene            complement(2273..>2799)
                     /locus_tag="VFA35_02905"
     CDS             complement(2273..>2799)
                     /locus_tag="VFA35_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZT55147.1"
                     /translation="SAIAAASVPASAFAVDYMTADQAAKLIFPDADAFEARSVALDAT
                     QLQQLDAQGVRGRSATWPVRVAKKAGATLGFVVTDEVIGKVELISYAVGIALDGNIRQ
                     VEILSYRESHGFEIRLPAWRRQFVGKGAGSALRVGDDIANISGATLSCTHVTDGVRRI
                     VAVVALARRGGGLP"
BASE COUNT          400 a          981 c          978 g          440 t
ORIGIN      
        1 tcgtcattcc ggcgctggtg ctgctggtct ggttgggcat ggccaggcgc tggccgttcg
       61 atcactggac tggatgccgg gatgggcgat gttcgccgtg gtcgtgcttc cctggttcgt
      121 cgctgcggag tgggcgcacc cggggttcct ccactacttc ttcgtggtgc agcagcttca
      181 gcgctttgcg tcgtgcggct tcaccaatgc gcagccgttc tggttctacg cgccggtgct
      241 gttcctcttc acgttgccgt ggtcgggctg gtggcttgcc aacctgcgca agtcgacggc
      301 ggcgcgatcc tcccgggcca ctgccgtgtc gccgatgtgc gtgtgactgg gggcggtgct
      361 cgtcttcttc tcgattccgg aatcgaagct cgtgggctac gtgctgcggg cgctcccgcc
      421 gctggcgctg cccgtcgtgc tcacggcgca gccgctgatc gagcggtcgc gcagggcagc
      481 gacgctgtgg cgcactgcac gacccttgct gccgtcggct gcgttgccgg ggtcgtgggt
      541 gttgcgctcg cgcattcgaa atcctcccgg gcgctggcgc tcggtcccag cacgtggtgc
      601 acggtggcgt cgcgttcgtc gacgagtacc gctacgacgt gccgttccat ctgcgtgaca
      661 cgtcgccggt gatggtggtg tcgaattggg atcccgccga ggttcgacag cacgacaact
      721 ggcgcaagga aatggccgac gccgcgggct ttgcgcccac cgccggtgcc gaccggctgc
      781 tcgacgaaaa cgagttcgag gcggccctct gcaccgggcg catccactgg gtcatcgcgg
      841 ggctcgacag cgcaaagcac catcacttcc tggaggtggc gcagcgcatt gccacggacc
      901 gcgacagcgc gttgtggcag gtcgatgcgg cgcgtccggc gcttgccgcc gccgcgggct
      961 gcgcaggcgc gccgctcgcg aagccggatt cgccaggccg accccggtct cgttgacttc
     1021 agcggggctc cgggcgtgcc gccgcctcgt cccgttcccg ttgcccgcgg cgatggtgct
     1081 gcagcaggca ggccatcgac acgccaacga ccgcgtgagc ccaaccgagg gcagggcgca
     1141 cgccctcggg cgcgaagtag tagagcgcgt agccggtgag cgcaagcgcc gcgccggctg
     1201 cacacagcat caagcccgtg cgacgctggc cggaccagcg acgccggtgg acgagccgcc
     1261 agccctgcgg aatgtgcgtt gccgcgagcg cacccagcgt gaacagcccg caaaacgccg
     1321 cgagcccgtg cacccgcatg caccaggctt cgagcggatg cggcagctcg cccgcgcccg
     1381 cgccgacgct gtagtggacg gcgagccaaa tgccccccga gagcagcagt gcaacaccac
     1441 ttgcatagag cgcgatgcgg tgccagcgaa cgagacggtg atggctgtga cgcggtgtcg
     1501 cgccagagga acggctcatg aattcagtgc tgccaagcgc tggcaccgaa gtgggccagc
     1561 accgggtgcg cagtatcgcc gctcgccgcg acgaccttcg tgagcgcatc ggcccacagg
     1621 cagcatggcg cggcgacgct gacatgtgcc tgcacgcttc gcaccccacg gtgggcaagc
     1681 gacgagcggg tctcgcgccc gaagtggctg gtggcgaatg cgccgtcgcg cagcgtcgcg
     1741 aaatgccgca cgccgccgcg ccgctcgtcg cgcaggccga tcggcagttc ggcgtctccg
     1801 aaggcgcgca gatcgccgcc cgcgttgacc cagcccgagc cgacgccggc ggcgaccagg
     1861 gcctgcacgg cgtggtcgat cgcaaagcct ttgccaatcc cgccgaggtc gagtcgcgta
     1921 caaccggcga gcttgaccag ccggttgccc tcgatgcgcc agccgccggg tgcgctgccg
     1981 aggctgatgt cgaacaagcc gtcggtcagg cgttccagtt gcgatgccgc cttcagcact
     2041 tcggcggtca gtgggcgcat ggcgagcgat tcaccgcgcc tgagcccgtt gaagcgcgcg
     2101 atgtcgctcg cgcgctcata gcgtgacagg cacgcctgca ccgccgcgat caccgcgaag
     2161 ccggcatccg acgcatgttt gtcggcgcgg ccgtcgacga tgccgatctc gaccagcgtg
     2221 cccagcagcg gctgagcgcg ccgccgccag ccatgcgcgg ccggcgccgc gatcatggca
     2281 gcccgccgcc gcgccgcgcc agcgcgacca cggcgacgat ccggcgcacg ccgtcggtca
     2341 cgtgcgtgca actgagcgtg gcgccgctga tgttggcgat gtcgtcgccg acgcgcagcg
     2401 ccgagcccgc acccttgccg acgaactgtc tgcgccacgc cggcagacga atctcgaagc
     2461 cgtggctctc gcggtacgac aggatctcga cctgccggat gttgccgtcc agcgcgatgc
     2521 cgaccgcgta gctgatcagt tcgaccttgc cgatgacctc gtcggtcacg acgaagccga
     2581 gcgttgcgcc ggccttcttc gcgacgcgca ccggccaggt cgccgagcgg ccgcgaacgc
     2641 cttgagcatc caactgctgc agttgcgtgg cgtcgagcgc gacgctgcgc gcctcgaacg
     2701 catcggcgtc gggaaatatc aatttggcgg cctgatccgc ggtcatgtag tcgaccgcga
     2761 acgcgctcgc gggcacagac gccgcggcga tggccgatg
//
LOCUS       DASRDG010000179         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_732492, whole genome shotgun sequence.
ACCESSION   DASRDG010000179 DASRDG010000000
VERSION     DASRDG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732492"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1283
                     /locus_tag="VFA35_02910"
     CDS             129..1283
                     /locus_tag="VFA35_02910"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZT55148.1"
                     /translation="MTANPGFDPARLDYLTAAVEREIAAGLYFGGVINVLRGGETGYC
                     KAFGHSSEARERAVALDSVFSLFSVTKAFTNVLVFQAIERGLLTLTTPLAKVIPEFGG
                     GLRERITVFHLLTHSAGLPTMFEARPGMYIDRLDEVIAAICEVALPIEPPGLSVGYAP
                     LFNHALLGEAVRRVDPRKRAYRDLMREDIFEPLGMRDTAMGVRRDLKARHLFPEFRGN
                     YPIKHLGHSDLGPNGAFAEEQAEMPWVGCVSTAADLMRFTEMLRLGGSLGGRRIVGRA
                     LIDRARTIWTGDKPNDFYASAIRAAGGIAPPANLGLGFSVRGPQMGTSMFGTLASQGT
                     FGAHGAGTTLFWVDPERDISFVGLMTGLMPSVDNLLRWQRLSDIVLSAAL"
     gene            1305..>2587
                     /locus_tag="VFA35_02915"
     CDS             1305..>2587
                     /locus_tag="VFA35_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZT55149.1"
                     /translation="MTRDGSFDYVIVGAGTAGCALAGLLAEDPATRVCVLEAGGSERH
                     PFVSTPAMVAPAIATARLNWRFETVPQPQLKGRRIPQPRGKVVGGSGSINGMVYMRGH
                     PRDYDDWAALGATGWDYAAVLPYFVRAENNEDLPASVYHGHGGRMNVRRPRRPNRLNS
                     AFIDATRALGYPPTSDFSGASSEGVGLRQGVIRGGRRESTARAYLRPALRRGNVCLLT
                     DALALRIKVGQGRATGVVYEQQGVQHEVGAGREVILCAGTLQSPQLLMLSGIGSTAEL
                     QRLGIAPVHELDGVGRNLHDHLACPVMMSSTDPTSYGISARALPRGILNVIEYLLLRS
                     GPLASNVFESVAFLKTAPGLDRPDVQFVFQPAARPKPTFPLPVGHGYAVSPVELYPRS
                     RGSVRLASADPHAAPLIDPALLSVESDIEPLVRAI"
BASE COUNT          378 a          938 c          860 g          411 t
ORIGIN      
        1 gcattgcacg cgaaaccttc gccgagctgg atgccgccgg ctgacgattg tatgtgttgc
       61 attcgaactg ggtggggaat agagtttccc cggcacccgc aaccgccaac aacgaggtac
      121 cggcgtacgt gaccgcgaac cccggcttcg atcccgcacg cctcgactac ctgaccgccg
      181 cggtcgagcg cgaaatcgcc gccggcctct atttcggcgg cgtcatcaac gtgctgcgcg
      241 gcggcgagac cggttactgc aaggccttcg gccacagcag cgaggcgcgc gagcgcgcgg
      301 tggcgctgga ttcggtgttc agcctgttct cggtcaccaa ggcgttcacg aacgtgctgg
      361 tgttccaggc gatcgagcgc ggcttgctga cgttgacgac gccgctcgcg aaagtgattc
      421 cggaattcgg cggcgggttg cgcgagcgca tcaccgtttt ccacctgctc acgcacagcg
      481 cggggctccc gaccatgttc gaggctcggc ccggcatgta catagaccgg ctggacgaag
      541 tcatcgccgc gatctgcgaa gtcgcgctgc cgatcgagcc gccggggctc agcgtcggct
      601 atgcgccgct gttcaaccac gcgctgctcg gcgaggccgt gcgccgcgtc gatccgcgca
      661 agcgtgccta tcgcgacctc atgcgcgagg acatcttcga accgctgggc atgagagata
      721 ccgcgatggg cgtgcggcgc gacctcaagg cgcgccacct gttcccggaa ttccgcggca
      781 attacccgat caagcacctc ggccactccg acctcgggcc caacggcgcc ttcgccgagg
      841 aacaggccga gatgccgtgg gtcggttgcg tctcgaccgc cgccgacctg atgcgcttca
      901 cggagatgct gcgcctcggc ggctcgctcg gcgggcggcg catcgtcggc cgcgcgctca
      961 tcgatcgcgc gcgcactatc tggaccggcg acaagcccaa cgatttctac gccagtgcga
     1021 ttcgtgcagc cggcggcatc gcgcctcccg ccaacctcgg cctcgggttc tcggtgcgcg
     1081 gcccgcaaat gggcacctcg atgttcggca cgctggcatc gcagggcacc ttcggcgcgc
     1141 acggcgccgg cacgacactg ttctgggtcg acccggagcg cgacatcagc ttcgtcggcc
     1201 tgatgaccgg gctcatgcct tcggtcgaca acctgctgcg ctggcagcgc ctgtcggaca
     1261 tcgtgctctc cgcagcgctc tagcccccgc gccgaacgcc cgcaatgacc cgcgatggca
     1321 gcttcgatta cgtcatcgtc ggtgccggga cggccggctg cgcgctggcg ggcctgctcg
     1381 ccgaagatcc cgcgacccgc gtctgcgtgc tcgaggccgg gggaagcgag cgccatccct
     1441 tcgtcagcac gcccgccatg gtggcgccgg ccatcgccac cgcgcgactg aactggcgct
     1501 tcgagaccgt cccgcaaccg cagctcaagg ggcgccgcat cccgcaaccg cgcggcaagg
     1561 tggtcggcgg ctcaggctcc atcaacggca tggtatacat gcgcggccat ccgcgcgact
     1621 acgacgactg ggcggcgctc ggcgccacgg gctgggacta cgccgcggtg ctgccgtact
     1681 tcgtgcgcgc ggagaacaac gaggacctgc cggcttcggt ctatcacggt cacggcggcc
     1741 gcatgaacgt gcgccggccg cggcgaccga accggctgaa ctccgctttc atcgacgcca
     1801 cccgcgcgct gggctatccg cccacctcgg acttcagcgg cgcaagctcg gaaggagtgg
     1861 gcctgcgcca gggcgtgatc cgcggcggtc gccgcgaatc gacggcgcgt gcctaccttc
     1921 gccccgcgct ccggcgcggc aacgtttgcc tgctgaccga cgcgctggcg ctgcgcatca
     1981 aggtcgggca gggccgcgcc accggcgtcg tgtacgagca gcagggagtg cagcacgagg
     2041 tcggcgccgg ccgcgaggtg atcctgtgcg ccgggaccct gcagtcgcca cagctgctga
     2101 tgctctcggg catcggttcg acggccgaat tgcagcgcct cggcatcgcg cccgtgcacg
     2161 agctcgatgg cgtcggccgc aacctgcacg atcacctggc ctgtcccgtg atgatgagca
     2221 gcaccgatcc gacctcttac ggcatctcgg cgcgtgcgct gccgcgcggc atcctgaacg
     2281 ttatcgagta cctgctgctg cgctcgggcc cgctggcgag caatgtcttc gagtcggtcg
     2341 cgttcctcaa gaccgcgccc gggctcgacc gcccggacgt gcagttcgtg ttccagccgg
     2401 ccgcgcggcc caagccgact ttcccgctgc cggtcggcca cggttacgcg gtcagcccgg
     2461 tggaactcta cccgcgcagt cgcggcagcg tgcgcctcgc cagcgccgat ccgcacgcgg
     2521 cgccgctcat cgatccggcc ttgctcagcg tcgagtcgga catcgagccg ctcgtgcgcg
     2581 cgatcaa
//
LOCUS       DASRDG010000180         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1059209, whole genome shotgun sequence.
ACCESSION   DASRDG010000180 DASRDG010000000
VERSION     DASRDG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059209"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFA35_02920"
     CDS             <1..394
                     /locus_tag="VFA35_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019452111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55150.1"
                     /translation="NRAAGAGEQRNAQASNVNADKRTNEVRNTSVNNVNVNNNNNVNV
                     NKNVNVNVQNNGGCCNNGWDNDYHPLATAAAVTATVAVTSAVIGSMVNTVPPSCVPVN
                     YGGMVYQQCGTTWYQSQGPQYVVVNPPY"
     gene            complement(447..1880)
                     /locus_tag="VFA35_02925"
     CDS             complement(447..1880)
                     /locus_tag="VFA35_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZT55151.1"
                     /translation="MEFRILSHAAMWVRCGGTSVVIDPWLVGSCYWRSWWNFPRAVFD
                     EREMAAVDAVILSHQHWDHWHGPSLRKLFAGKPVLIPDEPALRSGRDLRAIGFRDVRR
                     VPHGRTVEIGTLRITLYQFGRYVNDSAIVVEGGGVTLLNANDAKIAGWPLRHLVARHG
                     PIDFAFRSHSSANARVCWRLEGDTGYVVDDHEHYMRSFVQFMDAVQPRFAVPFASNHC
                     HLHDDVFAFNDMIATPIELREHVQSLPPRRWALQVMLPGSRWQQGPGGEPGRFDLAPE
                     TPYDDMPATLRAYRAEQAPALERFRQQENGVVVSDALLARFLDFFRAARARPPAPLGA
                     LKLVLRWPDGRRQAHAIDLDRWTVAPAQEAARAEPGLPMLEMPAIVFRDAVVKNMFGH
                     AGISKRCAFVASNHDDLRRLGAIVTHLDRHELGLYPVSWRYRARLVLAYVRRWRETVV
                     YAQALWLLKVKKLPIWLVEEAILKRSG"
     gene            1894..2145
                     /locus_tag="VFA35_02930"
     CDS             1894..2145
                     /locus_tag="VFA35_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55152.1"
                     /translation="MRRLLRRRLGHADCRSLRMNAPHDPRNAEAEIFRNFGLACRAER
                     DRLSLADLAELDRRFENGAIDRAIAELFVARLIATISRS"
BASE COUNT          387 a          760 c          749 g          302 t
ORIGIN      
        1 caatcgcgca gccggtgccg gcgagcaacg gaacgcgcag gccagcaacg tcaatgccga
       61 caagcgcacg aacgaggtgc gcaataccag cgtcaacaac gtcaacgtca acaacaacaa
      121 caacgtcaac gtcaacaaga acgtcaacgt caacgtccag aacaacggcg gctgctgcaa
      181 caacggctgg gacaacgact atcacccgct cgcaacggcc gcggccgtca ccgccaccgt
      241 agccgtgacc tcggccgtca tcggctcgat ggtgaacacc gtgccgccga gttgcgtacc
      301 ggtgaactac ggcggcatgg tctaccagca gtgcggcacc acctggtacc agtcgcaggg
      361 gccgcagtac gtcgtcgtca atccacctta ctgacaaacg cgcgcagcga gctgccaccg
      421 ggtcccgggg cggctcgctc actgcgctac ccgctgcgct tcaggatcgc ctcctccacc
      481 agccagatcg ggagcttctt caccttgagc agccacagcg cctgcgcgta gaccacggtc
      541 tcgcgccaac gccgcacgta ggcgagcacg aggcgcgcgc ggtagcgcca ggagaccggg
      601 tagaggccga gctcgtgccg gtccagatgg gtgacgatcg caccgagccg acgcaggtcg
      661 tcgtggttgc tcgcgacgaa ggcgcagcgc ttggagatgc cggcgtggcc gaacatgttc
      721 ttgacgacgg cgtcgcgaaa gacgatggcc ggcatctcga gcatcggcag gccgggctcg
      781 gcgcgggccg cctcctgcgc cggcgccacg gtccatcggt cgaggtcgat ggcgtgcgcc
      841 tggcggcggc catcgggcca gcgcagcacc agcttgagcg cgcccagcgg cgccggaggc
      901 cgggcgcggg cggcgcggaa gaagtcgaga aagcgcgcca gcagcgcatc gctcacgacc
      961 acgccgttct cctgctggcg gaaccgctcc agggccggcg cctgctcggc gcgataggcg
     1021 cgcagcgtcg ccggcatgtc gtcgtatggc gtctcgggcg ccagatcgaa gcggccgggc
     1081 tccccgcccg ggccttgctg ccaacgcgag ccgggcagca tcacctgcag ggcccagcgc
     1141 cgcggcggca ggctctgcac gtgctcgcgc agctcgatcg gggtggcgat catgtcgttg
     1201 aaggcgaaca cgtcgtcgtg caggtggcag tggttggaag cgaacggcac cgcgaagcgc
     1261 ggctgcaccg cgtccatgaa ctgcacgaac gagcgcatgt agtgctcgtg gtcgtcgacc
     1321 acgtagccgg tgtcgccctc gagccgccag cagacccgcg cgttcgccga cgagtgcgag
     1381 cggaacgcga agtcgatcgg cccgtgccgg gcgacgaggt ggcgcagcgg ccagccggcg
     1441 atcttggcgt cgttggcatt gagcagcgtg acgccgccgc cttcgacgac gatcgccgag
     1501 tcgttgacgt agcggccgaa ttggtagagc gtgatccgca gcgtgccgat ctccaccgtc
     1561 cgcccgtgcg gcacgcggcg cacgtcgcga aagccgatcg cgcgcaggtc gcggccgctg
     1621 cgcagcgccg gctcgtcggg aatcagaacg ggcttgccgg cgaagagctt gcgcaagctc
     1681 gggccgtgcc agtggtccca gtgctgatgg ctgaggatga ccgcatccac cgctgccatc
     1741 tcgcgctcgt cgaacacggc gcgagggaaa ttccaccacg accgccagta gcacgagccg
     1801 accagccacg gatcgatgac cacgctggtt ccgccgcagc gcacccacat cgcggcgtgc
     1861 gacaagatcc ggaactccat gtcactattg tgcgtgcggc ggctgctccg ccggcgcctt
     1921 gggcatgccg attgccggag cttgcgaatg aacgcacctc acgatccccg caacgcggaa
     1981 gccgagatct ttcgcaactt cgggctggcc tgccgggccg agcgcgaccg cctgtcgctg
     2041 gccgacttgg ccgagctgga ccgccggttc gagaacggag cgatcgaccg ggcgattgcc
     2101 gagctgttcg tcgcgcgcct gatcgcgacg atctcgaggt cctagatgaa cgagctcgac
     2161 gccctgcgcg ccaaggtcga ccagttgcaa ggcggcct
//
LOCUS       DASRDG010000181         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1059379, whole genome shotgun sequence.
ACCESSION   DASRDG010000181 DASRDG010000000
VERSION     DASRDG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059379"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /gene="tsf"
                     /locus_tag="VFA35_02935"
     CDS             complement(<1..424)
                     /gene="tsf"
                     /locus_tag="VFA35_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZT55153.1"
                     /translation="MAAITASMVGELRAKTDAPMMECKKALTEAEGDMERAEELLRVK
                     LGSKAGKAASRITAEGVIVAAVVGNAGALVEVNCETDFTSKNDMFLAMANACAKLVAE
                     NNPADVAALSALAYTQDGYGPTLEDVRKGLIGTIGENMT"
     gene            complement(521..1267)
                     /gene="rpsB"
                     /locus_tag="VFA35_02940"
     CDS             complement(521..1267)
                     /gene="rpsB"
                     /locus_tag="VFA35_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZT55154.1"
                     /translation="MTVTMREMLEAGVHFGHQTRFWNPKMAPYIYGHRNKIHIINLEK
                     TQPLFNDAMKFIRQTAAKRGTILMIGTKRQAREVINAEAKRAGMPFVDQRWLGGMLTN
                     FKTVKGSLKKLKDMKAQVEAGTEIRIKKEALMFERELLKLEKDIGGIQDMNALPDAMF
                     VIDVGYHKIAILEAKKLGIPVIGIVDSNHSPEGIDYVIPGNDDSSKAVALYAKAVADA
                     VLEGKANATNEVVQAAAAEGDEFVEVSEGA"
     gene            1503..>2299
                     /locus_tag="VFA35_02945"
     CDS             1503..>2299
                     /locus_tag="VFA35_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT55155.1"
                     /translation="MKVAVIGAGIIGVSTAYELAADGHEVAVFERRGSVAAEASFANA
                     GVIAPGYWMPWTAPGMPKTLLGQLLGRHAPVRVHAGLGAGEIAWLWRWRRASKLGGGQ
                     ANRLRMQRLASFSRERLHRLTRELKLDYERGDGCLMLLRTPKDQALAQTSVASLAELD
                     TRFEMLDAGQCRAIEPGLCDETPLHGGVYLPDAEVGNCRQFTTLLRTEAQRAGVRFRF
                     HTQVQQIVPGRTPQLVHLYAPPQDSTVLMSAAAESAPNDAQDTQPMAL"
BASE COUNT          356 a          773 c          733 g          437 t
ORIGIN      
        1 tcgtcatgtt ctcgccgatc gtgccgatca ggcccttgcg cacgtcttcg agcgtcgggc
       61 cgtagccgtc ttgcgtgtac gccagcgccg acagcgcggc cacatcggcc gggttgttct
      121 cggcgaccag cttcgcgcag gcgttggcca tcgccaggaa catgtcgttc ttcgacgtga
      181 agtcggtctc gcagttgacc tcgaccagcg cgcccgcgtt gccgaccacg gccgcgacga
      241 tcacgccctc ggcggtgatg cgcgacgcgg ccttgccggc cttgctgccg agcttgacgc
      301 gcagcagctc ttccgcgcgc tccatgtcgc cctcggcctc ggtcagcgcc ttcttgcatt
      361 ccatcatcgg ggcgtcggtc ttggcgcgca gttcgccgac catgcttgca gtgattgcag
      421 ccattctcag taactcctcg tgttcattgg aagaaaaggg gccagcggcc cctttctctc
      481 ggtgcccggc gctcgcgcaa agcgagccgg gctcggggca tcaagcgcct tcgctgactt
      541 cgacgaattc gtcgccctcg gcggcagcgg cctgcacgac ttcgttcgtc gcgttggcct
      601 tgccttcgag caccgcgtcg gccacggcct tcgcgtacag cgcgacggcc ttcgacgagt
      661 cgtcgttgcc cgggatcacg tagtcgatgc cttccggcga gtgattcgag tcgacgatgc
      721 cgatgaccgg aatgccgagc ttcttggctt cgaggatcgc gatcttgtgg tagccgacgt
      781 cgatcacgaa catcgcgtcg ggcagcgcgt tcatgtcctg gatgccgccg atgtccttct
      841 cgagcttcag cagttcgcgc tcgaacatca gcgcctcttt cttgatgcgg atttcggtgc
      901 cggcctcgac ctgcgccttc atgtccttca gcttcttcag cgagcccttg accgtcttga
      961 agttggtcag catgccaccg agccagcgct ggtcgacgaa gggcatgccg gcacgcttgg
     1021 cttcggcatt gatcacttcg cgcgcctggc gcttggtgcc gatcatcagg atcgtgccgc
     1081 gcttggcggc ggtctgacga atgaacttca tcgcgtcgtt gaagagcggc tgcgtcttct
     1141 cgaggttgat gatgtggatc ttgttgcgat ggccgtagat gtacggcgcc atcttcgggt
     1201 tccagaagcg ggtttggtga ccgaagtgga caccggcttc cagcatttca cgcatggtga
     1261 cagtcatgag gactccaaag gttggttcta gaatccggct cgaatcgcga catacttgtc
     1321 ggaacacccg cgacacactt gtcgcaagca ttcaccacag gcttgtcgca acacctttaa
     1381 gcagccagtt cgcgatttgc ttggggaaag aggtccgaca ccacgtcgca cccagccaag
     1441 ccgatcgatt atagcattct catgacccct gacctgaccg gctcgcgcga gcgcttccct
     1501 gcatgaaggt cgccgtcatc ggcgccggca tcatcggcgt gagcacggcc tacgagctcg
     1561 ctgccgatgg ccacgaggtg gcggtgttcg agcgccgcgg cagcgtcgcc gccgaagcga
     1621 gtttcgcgaa tgccggcgtc attgcacccg gctattggat gccatggacc gcccccggca
     1681 tgccgaagac gctgctcggc cagctgctgg gtcggcacgc gccggtgcgc gtgcacgccg
     1741 gcctcggcgc cggcgagatc gcgtggctgt ggcgctggcg gcgcgcgagc aagctcggcg
     1801 gcggccaggc caaccggctg cgcatgcagc gactcgccag cttcagccgc gagcggctgc
     1861 accggctgac gcgcgagctg aagctggact acgagcgcgg cgacggctgc ctcatgctgc
     1921 tgcgcacgcc gaaggaccag gcactggcgc aaaccagcgt ggcctcgctg gccgagctcg
     1981 acacgcgctt cgagatgctc gatgccgggc aatgccgcgc catcgaaccc ggcctgtgcg
     2041 acgagacgcc gctgcacggc ggcgtctacc tgcccgacgc cgaggtcggc aactgccggc
     2101 agttcacgac gctgctgcgc accgaagcgc agcgcgccgg cgtgcgcttt cgcttccaca
     2161 cgcaggtgca gcaaatcgta ccgggccgca cgccgcaact cgtccacctg tatgcgccac
     2221 cgcaggattc gaccgtgctg atgagtgcgg ctgccgagag cgcgccgaac gacgcgcagg
     2281 acacgcagcc gatggcgct
//
LOCUS       DASRDG010000182         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_831025, whole genome shotgun sequence.
ACCESSION   DASRDG010000182 DASRDG010000000
VERSION     DASRDG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831025"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VFA35_02950"
     CDS             <1..1476
                     /locus_tag="VFA35_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55156.1"
                     /translation="LAWRAFCAGNDTAALGLLQGVGGDDVQRRRIAEHERAIRAGART
                     ADAMVRALALGPPFPGLRRNGTVETLSAVQAADGVRLQATVASGAARTAHVTEFAFAD
                     LWREVLRRKPQLDVPASELELGRAVLAMIGDELGAGGSALQPGDGASATMPFLRDEVY
                     ERIVRVRRETDAGALAFVDALRAVDDAYARARKSHDHDLHDLDDALAVFSDRLLQAAT
                     AEDRAHLSEMREWRDAEHNRQRTRDLVRSGAPKGARVDTTANGTDMTVRLEGEQLQAI
                     ATDSWVLNRDTGLLEFRGVAAQRADEMVRGRLGDVGSGLPATVLRSTCTIDLVLPPAS
                     RGQRLYVFDYRGVGFLLLITADDFARAVTIEGDPRKEEAVHAAVARILGAPIDRELQL
                     LHPALGAPVADPRARVVPSAVHRVELSVDVSPARRDGALRNAVARLRFDAPAGSDVHP
                     DPYELCRDEQVEIDAARLPNLTIYPLQVMHLRAVEFLGHGL"
     gene            complement(1477..>2116)
                     /locus_tag="VFA35_02955"
     CDS             complement(1477..>2116)
                     /locus_tag="VFA35_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55157.1"
                     /translation="TEDDLLAARSDLQAARALDPTLPGVDYRIGQLDRALGQDELAVP
                     RLRLAAALAADPALRAAAADEAGQSLARRDDAARGQQLRRDAHRRLGAGDARSAEALA
                     LQARAEEPDPVADLRLRHRLAEAHNDVRGALGTALLLREYAGDARAEQLLADDLDQLG
                     CDGLARRAGLRARGLDMPAADLLAALAVYGDFANAAFLRWVDRTAGLAAPAR"
BASE COUNT          252 a          785 c          820 g          259 t
ORIGIN      
        1 ctcgcgtggc gcgcgttctg cgccggcaac gacacggccg cgctcgggct cctgcaaggc
       61 gtcggcggcg acgacgtcca gcggcgccgc atcgccgagc acgagcgcgc gatccgcgcc
      121 ggggcgcgca ccgccgacgc gatggtgcgc gcgctggcgc tcggaccgcc gttccccggg
      181 ctccggcgca acgggaccgt cgagacgctc agcgccgtgc aggccgccga cggcgtgcgg
      241 ctgcaggcga cggtcgcgag cggcgccgcg cgcaccgcgc acgtgacgga gttcgcgttc
      301 gcggacctct ggcgcgaggt cctgcggcgc aagccgcagc tcgacgtccc ggcgtcggaa
      361 ctggagctcg gccgcgcggt gctggcgatg atcggcgacg aactgggcgc gggaggcagc
      421 gcgctgcagc ccggcgacgg cgcgtcggcg acgatgccct tcctgcgcga cgaggtctac
      481 gaacgcatcg tccgcgtgcg ccgcgagacc gacgcgggcg cgctcgcgtt cgtcgatgcg
      541 ctgcgcgcgg tggacgacgc ctacgcccgc gcgcgcaagt cgcatgacca tgacctgcac
      601 gacctcgacg acgcgttggc cgtgttcagc gaccggctgc tgcaggcggc gaccgccgag
      661 gaccgcgcgc acctgtcgga gatgcgcgag tggcgcgatg cggagcacaa ccgccagcgc
      721 acgcgcgatc tcgtccgcag cggcgcaccc aagggcgccc gcgtcgacac caccgccaac
      781 ggcaccgaca tgaccgtgcg gctcgaaggc gaacagctgc aggcgatcgc gaccgacagc
      841 tgggtgctca accgcgacac gggcctgctc gagttccgcg gcgtggctgc ccagcgcgcc
      901 gacgagatgg tgcgcggccg cctcggcgac gtcggcagcg gcctgccggc gacggtcctg
      961 cgctccacct gcacgatcga cctggtgctg ccgccggcgt cgcgcggtca gcggctctac
     1021 gtgttcgact accgcggcgt cgggttcctg ctgctgatca cggccgacga cttcgcacgc
     1081 gccgtgacga tcgagggtga tccgcgcaag gaggaggccg tgcacgcggc ggtcgcgcgc
     1141 atcctcggcg cgccgatcga tcgcgagctg cagctgctgc atcccgcgct cggcgcgccc
     1201 gtcgccgatc cgcgcgcgcg cgtcgtgccg tccgccgtgc accgcgtgga gctgtcggtc
     1261 gacgtctcgc ccgcgcgccg cgacggtgcg ctgcgcaacg cggtcgcgcg gctgcggttc
     1321 gacgctcctg ccggatccga tgtgcatccc gatccgtacg agctgtgccg cgacgagcag
     1381 gtggagatcg atgccgcgcg gctgcccaac ctgacgatct atccgttgca ggtgatgcac
     1441 ctgcgcgcgg tcgagttcct cgggcacgga ctctgatcag cgcgccggcg ccgccaatcc
     1501 cgcggtgcgg tcgacccagc gcaggaacgc ggcgttggcg aagtcgccgt agacggcgag
     1561 cgcggccagc aggtcggcgg cgggcatgtc gaggccgcgc gcgcgcaggc cggcgcgccg
     1621 cgcgagaccg tcgcagccga gttggtcgag gtcgtcggcc aagagctgct cggcgcgcgc
     1681 gtcgccggcg tattcgcgca gcagcagcgc cgtgcccagc gcgccgcgga cgtcgttgtg
     1741 cgcttcggcg aggcggtgcc gcaggcgcag gtcggcgacg gggtcgggtt cctcggcgcg
     1801 cgcctgcagc gccagcgcct ccgccgaacg cgcgtcgccc gcgccgaggc ggcggtgcgc
     1861 gtcgcggcgc aactgctggc cgcgtgcggc gtcgtcgcgg cgcgcgagcg attgcccggc
     1921 ctcgtcggcg gccgcggcgc gcagcgccgg gtccgcggcc agagcggcgg cgaggcgcag
     1981 gcgcggcacg gcgagttcgt cctggcccag cgcgcgatcg agctggccga tccggtagtc
     2041 gacgccgggc agcgtcgggt cgagggcgcg cgccgcctgc aggtcgctgc gcgccgccag
     2101 caggtcgtcc tcggtg
//
LOCUS       DASRDG010000183         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_598137, whole genome shotgun sequence.
ACCESSION   DASRDG010000183 DASRDG010000000
VERSION     DASRDG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598137"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..477
                     /locus_tag="VFA35_02960"
     CDS             124..477
                     /locus_tag="VFA35_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZT55158.1"
                     /translation="MKIIVRWLLLAAALLLVAYLYPGVTVNSFASALIAAFVLGLLNT
                     LLRPVLVLLTLPVTLITLGLFLFVINALMFYFAASMLDGFHVAGFFAALVGSLLYSLC
                     GMVIDVAIERVFARG"
     gene            complement(478..2589)
                     /gene="pbpC"
                     /locus_tag="VFA35_02965"
     CDS             complement(478..2589)
                     /gene="pbpC"
                     /locus_tag="VFA35_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HZT55159.1"
                     /translation="MNRAAAALLLALGLGVTSANAAPLPSFAAVKTAHRPSDITLLDR
                     HGVPIQTVRVDKEVRRLAWVPLAEMSPALVTAIVLSEDQRFWEHSGVDWGAAARSAWG
                     NLIDTRTRGASTLTMQLAGLLDDGLARPGGGRSVGQKIGQAITAARLEARWTKAEILE
                     AYLNTVAFRGEVVGIAALSQTLFGKHPSGLDAQEAALAAALLRAPNARADAVAQRACG
                     VLRLQHAPCGGVVALTATALARQGGMPLGEQLAPHFARQVVAADGAVLQRSTLDAGLQ
                     RVAVTALRRQLGELAGRNVEDGAVVVLDNASGEVRAWVGSSGARSGADQVDGVLARRQ
                     PGSALKPFVYELAFEQRLITPASLIDDSPAQLATTNGLYLPQNYDHDFKGWVSARTAL
                     GASLNVPAVRVGAMLGPDALVERFNALGLALPKPGGFYGSSLALGSADVTLLALTNAY
                     RALANGGLYAPVALRSASTSVKPVRVAAAAAVFLVTDILADNNARARTFGLNSLLATG
                     GFAAVKTGTSKDMRDNWCVGYTERYTVGVWVGNAGGAPMHGVSGVSGAAPVWQALVAQ
                     LHAGQPSRAPRPPGGVVRARVAFETQREPARDEFFIAGTEQAVLRASAPRLGIASPRD
                     GSIFAIDPDMPPAAQRIRFEGEQGTWVLDGKRLGAATQLGWAPWPGRHELALLGRDGA
                     VTDSVRFEVRGAGVKTALERR"
     gene            complement(2586..>3098)
                     /locus_tag="VFA35_02970"
     CDS             complement(2586..>3098)
                     /locus_tag="VFA35_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55160.1"
                     /translation="TLTVAQQGSGKPWLTVQGLAAIPLKAPLAAGYSIARSVSAVEQK
                     DKARWSRGDIVRVRLEINAQADMTWVVVSDPVPGGATILGSGLGRDSALATRTEKRAG
                     SAWTAFEERGFEAYRSYYEYLPRGKHVVEYSVRLNNPGRFALPPTRVEAMYAPESFGE
                     VPNASIEVAP"
BASE COUNT          399 a         1156 c         1092 g          451 t
ORIGIN      
        1 attcgaggcg gaatcggtgg acggaggagg cggccaagaa atttaacaca aatgagaacg
       61 attcccattg caagcccgga ccgcgcgcgg ggacgctgca ccggccgcgg tattcttcgg
      121 gccatgaaaa tcatcgttcg ctggctcttg ctcgccgccg ccctgttgct ggtggcctac
      181 ctctaccccg gcgtcacggt gaacagcttc gcctcggcgc tgatcgccgc cttcgtgctc
      241 gggctgctga acacgctact gcggccggtc ctggtgctgc tgacgctgcc ggtcacgttg
      301 atcacgctcg gcctgttcct gttcgtgatc aacgcgctga tgttctattt cgccgcgtcg
      361 atgctcgacg gcttccacgt cgccggcttc ttcgccgcgc tggtcggctc gctgctgtac
      421 agcctgtgcg gcatggtgat cgatgtcgcc atcgagcgag tcttcgcccg cggctgatca
      481 gcgacgctcg agggccgtct tcacgcccgc gccgcgcacc tcgaagcgca cgctgtcggt
      541 caccgcaccg tcgcgcccca gcagggccag ttcgtgccgc cccggccacg gtgcccaccc
      601 gagctgggtc gccgcgccca gccgcttgcc gtcgagcacc caggtgcctt gctcgccctc
      661 gaagcggatg cgctgcgccg ccggcggcat gtcggggtcg atcgcgaaga tgctgccgtc
      721 gcgcgggctg gcgatgccca agcgcggcgc gctcgcgcgc agcacggcct gctcggtgcc
      781 ggcgatgaag aactcgtcac gcgccggctc gcgctgcgtc tcgaacgcga cccgggcgcg
      841 caccacgccg ccgggcgggc gcggtgcgcg cgagggctgc ccggcgtgca actgcgcgac
      901 cagcgcctgc cacaccggcg ccgcgccgct cacgccgctc acgccatgca tcggcgcgcc
      961 gccggcgttg ccgacccaca cgccgaccgt gtagcgctcg gtgtagccga cgcaccagtt
     1021 gtcgcgcatg tccttgctgg tgccggtctt caccgccgcg aagccgcctg tggcgagcag
     1081 gctgttcagg ccgaaggtcc gtgcgcgggc gttgttgtcg gcgaggatgt cggtgacgag
     1141 gaacacggcg gccgcagcgg cgacgcgcac cggcttcact gacgtcgatg ccgaccgcaa
     1201 cgcgaccggc gcgtacaggc ccccgttcgc gagcgcgcgg tacgcgttcg tcagcgcgag
     1261 cagcgtcacg tcggcgctgc cgagcgcgag cgagctgccg tagaagccgc ccggcttcgg
     1321 cagcgccagg ccgagcgcgt tgaagcgctc gaccagcgca tcgggcccga gcatcgcgcc
     1381 gacgcgcact gccggcacgt tcaggctcgc gccgagcgcg gtgcgggcgc tgacccaacc
     1441 cttgaagtcg tggtcgtagt tctgcggcag gtacaggccg ttcgtcgtcg cgagctgcgc
     1501 cggtgagtcg tcgatcaggc tcgccggcgt gatcagccgc tgctcgaacg cgagctcgta
     1561 gacgaagggc ttcaaggccg agccgggctg gcgccgcgcg agcacgccgt cgacctggtc
     1621 cgcgcccgac cgcgcgccgc tcgagccgac ccacgcgcgc acctcgccgc tcgcgttgtc
     1681 gagcacgacg accgcgccgt cttcgacgtt gcggccggcg agctcgccga gctggcgccg
     1741 cagcgcggtg acggcgacac gctgcagccc ggcatcgagc gtgctgcgct gcagcaccgc
     1801 gccgtcggcc gcgaccacct ggcgcgcgaa gtgcggcgcg agctgctcgc cgagcggcat
     1861 gccgccctgg cgcgcgagcg cggtcgcggt caacgcgacg accccgccgc acggcgcgtg
     1921 ctgcaaccgc agcacgccgc aggcgcgctg cgccaccgcg tcggcacgcg cgttcggcgc
     1981 gcgcagcagc gccgcggcga gcgcggcctc ctgcgcatcg agcccgctcg ggtgcttgcc
     2041 gaacagcgtc tggctcagcg cggcgatgcc gacgacctcg ccgcggaacg cgaccgtgtt
     2101 caggtaggcc tcgaggatct cggccttggt ccagcgcgcc tccagccgcg ccgcggtgat
     2161 cgcctggccg atcttctggc caacgctgcg cccgccgccc ggccgcgcga ggccatcgtc
     2221 gagcaggccg gcgagctgca tcgtcagcgt cgacgcgccc cgcgttcggg tgtcgatcag
     2281 attgccccag gcactgcggg ccgccgcgcc ccagtcgacg ccgctgtgct cccagaagcg
     2341 ctggtcctcg ctcaggacga tcgcggtcac gagggccggc gacatctcgg caagcggcac
     2401 ccaggcgagg cggcgcacct ccttgtcgac ccgcacggtc tggatcggca caccgtggcg
     2461 atcgagcaag gtgatgtcgg agggccggtg cgccgtcttc accgcggcga agctcggcag
     2521 cggcgccgcg ttggcgctgg tgacgccaag cccgagcgcg agcagcagcg cggcggcggc
     2581 gcgattcacg gcgcgacctc gatgctcgcg ttaggcactt cgccgaagct ctcgggcgcg
     2641 tacatcgcct cgacccgcgt cggcggcaag gcgaacctgc ccgggttgtt gaggcgcacg
     2701 ctgtactcga cgacgtgctt gccgcgcggc aggtattcgt agtagctgcg ataggcctcg
     2761 aagccgcgct cctcgaacgc cgtccaggcc gagccggcgc gcttctcggt gcgcgtcgcc
     2821 agcgccgagt cgcggcccag gcccgagccg aggatggtcg cgccgcccgg caccgggtcg
     2881 ctgacgacga cccaggtcat gtcggcctgg gcgttgatct cgagccgcac ccggacgatg
     2941 tcgccgcgcg accagcgcgc cttgtccttc tgctcgaccg cgctgacgct gcgcgcgatg
     3001 ctgtagccgg ccgcgagcgg cgccttcagc ggaatcgccg cgaggccctg caccgtgagc
     3061 cacggcttgc cgctgccctg ctgtgcaacc gtgagcgt
//
LOCUS       DASRDG010000184         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_953803, whole genome shotgun sequence.
ACCESSION   DASRDG010000184 DASRDG010000000
VERSION     DASRDG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953803"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VFA35_02975"
     CDS             <1..1275
                     /locus_tag="VFA35_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT55161.1"
                     /translation="LEPLFAGVGAIIGAVRMEAARIEAEQAVREGRVALVAAAAAQRA
                     NAAKTEFLSRMSHELRTPLNAVLGFSQLLRMDDSDPLTPTQTERVRHIENAGTHLLAM
                     IDDVLDLSRIESGSMPLSLDTVSLALAVNEALALVGAQAVERGVRVRVEPWQPDRPSG
                     GQVRADHLRLRQVLLNLIGNAVKYNRRGGTVVIAWRPSVNGAQVELEVRDTGLGMSPE
                     QLAHLFEPFNRLGAETSGVEGTGIGLVISQRLVQTMGGELEVSSEKGVGSCFRVSLPA
                     APADAMHGVGAESAAAHTTFDELDGVRTVLYADDNALNVEVVLAVLRTRPGLRVVVAR
                     NGREALAAARREQPDLLLLDMHLGDISGLEVMQTLALDPALAHIPCVALSADAMPASI
                     ERAERAGFKAYLTKPLNVKSFLHCIDRVLAAT"
     gene            1457..1768
                     /locus_tag="VFA35_02980"
     CDS             1457..1768
                     /locus_tag="VFA35_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HZT55162.1"
                     /translation="MGAAALPAHADVFVRVAPPPLRAEVVPAARPGYAWAPGYWNWNG
                     RRHVWVRGAWVRARPGYVYHQPAWEQHDGRWTMRRGGWDRDGDGVPNRADRHPDNPYR
                     R"
     gene            complement(1756..>3506)
                     /locus_tag="VFA35_02985"
     CDS             complement(1756..>3506)
                     /locus_tag="VFA35_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT55163.1"
                     /translation="HFYIVTDRIAGVHPMLPVMRDPDGFIYYKEEVGGLVMGGFEPQA
                     KPWKMDPVPSDFQFQLLDEDWDQFEPLMTAAMHRTPCLETAQVKMLLNGPESFTPDGN
                     FILGEAPELRNYFVAAGFNSAGIANSGGAGRLMAEWIIGGAAPSDLWDVDIRRFGPFM
                     ANRKALAERTGETLGLHYAMRWPRQELETARPLRTSPLYDLLADKGAVFGSKNGWERA
                     NYFKPAGAHPAEPGLGRPQWLDWVIAEQRATREAVALYDQTSFSKLLLQGRDALAVLQ
                     RLCANEIDIAVDAMVYTALLNERGGFESDLTVIRQAPERFLIVTGSAQTTRDQDWINR
                     HIAPADHAFLTDVSAMFSVLSVMGPKARELLSRVSPDDLSPEALKFSRTREIDVGFAR
                     VRAARMSYVGGPGFELYVPIEMARHVYLALMEAGDELGIRDAGYYALDALRIEAGRRA
                     WGAELGPDETPFEAGLAFAVKLDKPANFIGKTALLKAQGEPLRKKLVSVVLDSPSHYA
                     WGGEALTLDGAPVGELASVGWSPKANACVALAYLRGAAAQQAHAGTRAAIDLWGEPVG
                     ATVWDAWPPRPTGQRR"
BASE COUNT          484 a         1240 c         1265 g          517 t
ORIGIN      
        1 ctcgagccgc tgttcgccgg cgtcggcgcg atcatcggcg cggtgcgcat ggaggccgcg
       61 cgcatcgagg ccgagcaggc ggtgcgcgag ggccgggtcg cgctcgtcgc cgccgcggcc
      121 gcacagcgcg cgaacgccgc gaagaccgag ttcctctcgc gcatgagcca cgagttgcgc
      181 acgccgctca atgcggtgct gggcttctcg cagctgctgc gcatggatga ctccgacccg
      241 ctgacgccga cccagaccga gcgcgtgcgc cacatcgaga acgccggcac ccacctgctc
      301 gcgatgatcg acgacgtgct cgacctgtcg cgaatcgaga gcggcagcat gccgctgtcg
      361 ctcgatacgg tctcgctcgc gctggcggtg aacgaggcgc tggcgctggt cggcgcgcag
      421 gcggtcgagc gcggcgtgcg ggtccgcgtc gagccctggc agcccgatcg gccgagcggc
      481 ggccaggtgc gcgccgatca cctgcggctg cgtcaggtgc tgctcaacct gatcggcaat
      541 gccgtcaagt acaaccggcg cggcggcacg gtcgtgatcg cgtggcggcc ctcggtcaac
      601 ggcgcgcagg tcgagctcga agtgcgcgac accggcctgg gcatgtcgcc cgagcagctc
      661 gcgcacctgt tcgagccctt caaccgcctc ggcgccgaga cgagcggggt cgaaggcacc
      721 ggcatcggcc tggtgatctc gcagcgcctg gtgcagacga tgggcggcga gctcgaggtg
      781 agcagcgaga agggcgtcgg cagctgcttt cgcgtcagcc tgccggctgc gccggcggac
      841 gcgatgcacg gggtcggggc cgagagcgcc gcggcgcaca ccacgttcga cgagctggac
      901 ggcgtgcgca ccgtgctgta cgccgacgac aacgcgctca acgtcgaggt cgtgctcgcg
      961 gtgctgcgca cccggccggg gctgcgcgtg gtggtggcgc gcaacggccg cgaggcgctc
     1021 gcggcggcgc gccgcgaaca gcccgacctg ctgctgctcg acatgcacct gggcgacatc
     1081 tccggcctcg aggtgatgca gacgctggca ctcgacccgg cgctcgcgca tatcccgtgc
     1141 gtggcgctgt cggccgacgc gatgccggcc tcgatcgagc gcgccgagcg cgccggcttc
     1201 aaggcctacc tgacgaagcc gctgaacgtg aagagcttcc tgcactgcat cgaccgggtg
     1261 ctcgcggcaa cctagtcggc ccgagaccgg ccgccgttgc agccgcttgc aaggtctttg
     1321 caaagcttcg tgtgccgcgc tgccatccgc cgcgcgcctg cggcgttgat cgacatgaca
     1381 cctttgtcgt gcccttcacg gagcagaagt catgccgatc aagccttcgt tgtacgccgc
     1441 cgcgctcgca gccctgatgg gcgccgccgc gctacccgcc cacgccgacg tgttcgttcg
     1501 cgtcgcgccg ccgcccttgc gtgccgaggt cgtgccggcg gcccggcccg gctacgcgtg
     1561 ggcgccgggc tactggaact ggaacggccg ccgccacgtg tgggtccgcg gcgcctgggt
     1621 gcgcgcgcgc cccggctacg tctatcacca gccggcgtgg gagcagcacg acggccgctg
     1681 gacgatgcgc cgcggcggtt gggatcgcga cggcgacggc gtgccgaacc gcgccgaccg
     1741 gcacccggac aatccttatc gccgctgacc ggtgggtcgg ggcggccagg cgtcccacac
     1801 ggtcgcgccg accggctcgc cccacaggtc gatcgcggcg cgcgtgccgg cgtgcgcctg
     1861 ctgcgccgcc gcgccgcgca ggtaggcgag cgcgacgcag gcgttcgcct tcgggctcca
     1921 gccgaccgac gcgagctcgc cgactggcgc gccgtccagc gtcagcgcct cgccgcccca
     1981 tgcgtagtgc gacggcgaat cgagcacgac gctgacgagc ttcttgcgca gcggctcgcc
     2041 ctgcgctttc agcagcgctg tcttgccgat gaagttggca ggcttgtcga gcttgacggc
     2101 gaacgccaac ccggcctcga acggtgtctc gtcgggcccg agctcggcgc cccaggcgcg
     2161 gcggccggct tcgatgcgca gcgcgtcgag cgcgtagtag ccggcgtcgc ggatgccgag
     2221 ttcgtcgccc gcttccatca gcgcgaggta gacgtgccgc gccatctcga tcggcacgta
     2281 gagctcgaag cccggcccgc cgacatagct catgcgcgcc gcgcgcaccc gggcgaaacc
     2341 gacatcgatc tcgcgcgtgc gcgagaactt cagcgcctcg ggcgagaggt cgtcggggct
     2401 cacgcgcgac aacagctcgc gggccttcgg ccccatcacc gacagcaccg agaacatcgc
     2461 gctgacgtcg gtcaggaacg cgtggtcggc gggcgcgatg tggcgattga tccagtcctg
     2521 gtcgcgcgtc gtctgtgccg agccggtgac gatcaggaag cgctcgggcg cctggcggat
     2581 cacggtcagg tcgctctcga agccgccgcg ttcgttgagc agcgcggtgt agaccatcgc
     2641 atcgacggcg atgtcgatct cgttcgcgca caggcgctgc agcacggcga gtgcgtcgcg
     2701 gccctgcagc agcagcttcg agaacgaggt ctggtcgtag agcgcgaccg cttcgcgcgt
     2761 ggcgcgctgc tccgcgatga cccagtccag ccactgcggc cggcccaggc cgggctcggc
     2821 cggatgcgcg ccagccggct tgaagtagtt cgcgcgctcc cagccgttct tgctgccgaa
     2881 gaccgcgccc ttgtcggcga gcaggtcgta gagcggcgag gtgcgcagcg ggcgggcggt
     2941 ttcgagctcc tgccgcggcc agcgcatcgc gtagtgcagg ccgagcgtct cgccggtgcg
     3001 ctcggcgagc gccttgcggt tcgccatgaa cgggccgaaa cggcggatgt cgacgtccca
     3061 caagtcgctg ggcgccgcgc cgccgatgat ccactcggcc atcagccggc ccgcgccgcc
     3121 cgagttcgcg atgccggccg agttgaagcc ggccgcgacg aagtagttgc gcaactcggg
     3181 cgcctcgccg aggatgaagt tgccgtcggg cgtaaagctc tccgggccgt tgaggagcat
     3241 cttgacctgc gccgtctcga ggcagggcgt gcgatgcatt gccgcggtca tgagcggctc
     3301 gaactggtcc cagtcttcgt cgagcaactg gaactggaag tcgctcggga ccgggtccat
     3361 cttccacggc ttggcctgcg gctcgaagcc gcccatcacg aggccgccga cctcttcctt
     3421 gtagtagatg aagccgtcgg ggtcgcgcat caccggcagc atcgggtgca cgccggctat
     3481 gcggtcggtg acgatgtaga agtgct
//
LOCUS       DASRDG010000185         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_733351, whole genome shotgun sequence.
ACCESSION   DASRDG010000185 DASRDG010000000
VERSION     DASRDG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733351"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VFA35_02990"
     CDS             <1..762
                     /locus_tag="VFA35_02990"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429458.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZT55164.1"
                     /translation="RIGTVNSINWARLLAQVVYYFAGYFQATKDDGGTVSFAVPSGNF
                     GNICAGHVARMMGLPIRQLVLATNENDVLDEFFRTGTYRVRSSAETHETSSPSMDISK
                     ASNFERFVFDLLGRDAARTRQLFKTEIERSGAFTLTPEEFARIAGYGFVSGSSTHADR
                     LATIRDTDKRFGTVVDTHTADGLKVARQHLTPGIPTIVLETALPAKFAETIVEAIGRE
                     PERPAAMRGIEQLPKRFVVMPADAAQVKAFIAAES"
     gene            803..2017
                     /gene="glp"
                     /locus_tag="VFA35_02995"
     CDS             803..2017
                     /gene="glp"
                     /locus_tag="VFA35_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZT55165.1"
                     /translation="MLTLDEALARLIGAVAPLPSSQHETLSTFDALGRVLQADVRSTL
                     DVPPADNTSMDGYALRAADVPAAGTLLPVSQRIPAGVVGQPLQPGTAARIFTGAQVPA
                     GADAVVMQEQCEPVDEGVRVNAVPTPGQWIRRRGEDVRDGDVVLPAGARLTPQALGLA
                     ATVGAATLQVARRPRVALFSTGDELAMPGEPLKPGAIYNSNRFTLRAAIQSIGCECTD
                     LGIVPDRLDATRAALRRAAQGNDLIVTSGGVSVGEEDHLRPAVQAEGRIELWQIAIKP
                     GKPLAFGQVDRPDGSAAYVIGLPGNPVSSFLTFLAVVRPVLLRLQGATALTPKRSRQR
                     ADFDWPAPDKRREFLRVRRNDAGGLDLFPNQSSGVMTSVVWGDGVVDNPSGHAIAKGD
                     LVDFMTFAELLS"
     gene            2014..2265
                     /locus_tag="VFA35_03000"
     CDS             2014..2265
                     /locus_tag="VFA35_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZT55166.1"
                     /translation="MKLTVRYFASIREALGASEVVELAEGSSVGAVRDALIARGGVHA
                     SALARGKALRSALNQVLCDEAALVGEGAELAFFPPVTGG"
     gene            2267..2758
                     /locus_tag="VFA35_03005"
     CDS             2267..2758
                     /locus_tag="VFA35_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZT55167.1"
                     /translation="MNAPRVVVQTADFDLGAEVAALRSGDAGVGAVAAFIGTVRDRND
                     GLGVSAMELEHYPGMTERAIEAMIDEAQRRFEIRAARVIHRVGLLQPQNQIMMVAVTS
                     AHRGQAFLACEFLMDYLKTQAPFWKKEQTPEGARWVDARSSDDAAIARWGIEAGNAAP
                     EAP"
     gene            2755..3150
                     /gene="crcB"
                     /locus_tag="VFA35_03010"
     CDS             2755..3150
                     /gene="crcB"
                     /locus_tag="VFA35_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HZT55168.1"
                     /translation="MSGVSSLPWLQIVAVSIGAAFGAVARWLAGLWLNTRWEGFPLGT
                     LLVNCVGGLLIGMALAWFERTPNELLRLLLVTGFLGGLTTFSSFSGESLVMLHRGQFG
                     MALTHSGAHLLGSLFCAGFGYWVIDTLLD"
     gene            3225..>4053
                     /locus_tag="VFA35_03015"
     CDS             3225..>4053
                     /locus_tag="VFA35_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZT55169.1"
                     /translation="MRIDKLTTKFQEALADAQSIAVTNDNPYIEPVHVLAAMLAQTDG
                     PKALLDRAGVNSAALKTALDVALKGLPQVQGSGQVVQPSRDLAALLQTAEKEASKRGD
                     QFIASEMFLLALADAKTDLGGIVRANGMTRKALDAAIESVRGGQKVDNAEAEGQREAL
                     KKYTLDLTERARLGKLDPVIGRDDEIRRAIQVLQRRSKNNPVLIGEPGVGKTAIVEGL
                     AQRIVAGEVPDSLKHKRVLVLDMALLLAGAKFRGEFEERLKGVLKEVAQDEGQTIVFI
                     "
BASE COUNT          624 a         1424 c         1413 g          592 t
ORIGIN      
        1 cgcatcggca ccgtcaactc gatcaactgg gcgcgactgc tggcacaggt cgtctactac
       61 tttgccggct acttccaggc gacgaaggac gacggcggca ccgtgagctt cgcggttccg
      121 tcgggcaact tcggcaatat ctgcgcgggc cacgtggcgc gcatgatggg gctgccgatc
      181 cgccagctcg tgctcgcgac caacgagaac gacgtgctcg acgaattctt ccgcaccggc
      241 acctaccgtg tgcgcagcag cgccgagacc cacgaaacct cgagcccgtc gatggacatc
      301 tccaaggcct cgaacttcga gcgcttcgtg ttcgatctgc tcggccgcga cgcggcgcgc
      361 acgcgccagc tgttcaagac cgagatcgag cgcagcggcg cgttcacgct cacgcccgaa
      421 gagttcgcgc gcatcgccgg ctacggcttc gtctccggca gcagcaccca cgccgaccgg
      481 ctcgcgacga tccgcgatac cgacaagcgc ttcggcaccg tcgtcgacac ccacacggcc
      541 gacggcctga aggtggcgcg ccagcacctg acgccaggca tcccgacgat cgtgctcgaa
      601 accgcgctgc ccgcgaagtt cgccgaaacc attgtcgaag cgatcggccg cgaacccgag
      661 cgcccggccg cgatgcgcgg catcgagcag ttgccgaagc gcttcgtggt gatgccggcc
      721 gacgcggcgc aggtcaaggc cttcatcgct gccgaatctt gaacccgcga gcccgacccg
      781 catgcagccg caacgcgcac cgatgctgac gctggacgag gcgctcgccc gcctcatcgg
      841 cgcggtcgcg ccgctgcctt cgagtcagca cgagacgctc tcgaccttcg acgcgctcgg
      901 ccgcgtgctg caggccgacg tgcgctcgac cctcgacgtg ccgccggccg acaacacctc
      961 gatggacggc tacgcgctgc gcgcggccga cgtgcctgcg gccggcacgc tgctgcctgt
     1021 gagccagcgc atcccggccg gcgtcgtcgg ccagccgctg cagcccggca ccgccgcgcg
     1081 catcttcacc ggcgcgcagg tgccggccgg cgccgacgcg gtcgtgatgc aggagcagtg
     1141 cgagccggtc gacgagggcg tgcgcgtcaa cgcggtgccg acgcccggac aatggattcg
     1201 ccggcgcggc gaggacgttc gcgacggcga cgtcgtgctg ccggccggcg cgcggctcac
     1261 gccgcaggcg ctcggcctgg cggcgaccgt cggcgcggcc acgctgcaag tcgcgcgtcg
     1321 cccgcgcgtc gcactgttct cgaccggcga cgaactggcg atgcccggtg agccgctgaa
     1381 acccggcgcg atctacaact cgaaccgctt cacgctgcgc gccgcgatcc agtcgatcgg
     1441 ctgcgagtgc accgacctgg gcatcgtccc cgaccggctc gacgccaccc gcgccgcgct
     1501 gcgccgcgcg gcgcagggca acgacctgat cgtgacctcg ggcggcgtgt ccgtcggcga
     1561 agaagaccat ttgcgcccgg ccgtgcaggc cgagggccgc atcgaactct ggcagatcgc
     1621 gatcaagccg ggcaagccgc tcgcgttcgg ccaagtcgac cggccggacg gatcggcggc
     1681 ctacgtgatc gggctgccgg gcaacccggt gtcgagcttc ctcaccttct tggctgtggt
     1741 acggccggtg ctgctgcgcc tgcagggcgc gaccgcgttg acgcccaagc gcagccggca
     1801 gcgcgccgat ttcgactggc ccgcgcccga caagcgccgc gagttcctgc gcgtgcgccg
     1861 caacgatgcg ggcgggctcg atctgttccc gaaccagagt tcgggcgtga tgacctcggt
     1921 ggtctggggc gacggcgtcg tcgataaccc cagtggccac gccatcgcga agggcgatct
     1981 ggtcgacttc atgacgttcg cggagttgct gtcgtgaagc tcacggtgcg ttactttgcg
     2041 tcgatccgcg aggcgctcgg cgcgagcgag gtggtcgagc tcgccgaagg cagcagcgtc
     2101 ggcgcggtgc gcgatgcgct gatcgcgcgc ggcggcgtgc atgcgagcgc gctggcgcgg
     2161 ggcaaggcct tgcgcagcgc gctgaaccag gtgctgtgcg acgaagccgc gctcgtcggc
     2221 gaaggcgccg agctggcgtt cttcccaccg gtcaccggcg gctgaaatga acgcgccgcg
     2281 cgtcgtcgtg cagaccgccg atttcgacct cggcgccgag gttgccgcac tgcgttccgg
     2341 cgacgctggg gttggcgcgg tcgccgcatt catcggcacg gtgcgcgacc gcaacgacgg
     2401 cctcggcgtc agcgcgatgg agctcgaaca ctatcccggc atgaccgagc gcgcgatcga
     2461 ggcgatgatc gacgaggcgc agcgccgctt cgagatccgc gccgcgcgcg tgatccaccg
     2521 cgtcggcctg ctgcagccgc agaaccagat catgatggtc gccgtcacct cggcgcaccg
     2581 cggccaggcc ttcctggcct gcgagttcct gatggactac ctgaagaccc aggcgccgtt
     2641 ctggaagaag gaacaaacgc ccgaaggcgc gcgctgggtc gatgcgcgca gcagcgacga
     2701 cgcggcgatt gcgcgctggg gcatcgaagc cggcaacgcc gcgccagagg cgccgtgagc
     2761 ggcgtctcct cgctcccttg gctgcagatc gtcgcggtgt cgatcggtgc ggcgttcggc
     2821 gcggtcgcgc gctggctcgc cggcctgtgg ctgaacacac ggtgggaggg tttcccgctc
     2881 ggcaccttgc tcgtcaactg cgttggcggc ctgctgatcg gcatggcgct ggcgtggttc
     2941 gagcgcacgc cgaacgagtt gctgcgcctg ctgctggtca ccggatttct gggcgggttg
     3001 acgaccttct cgtcgttctc gggcgaatcg ctggtgatgc tgcaccgcgg ccagttcggc
     3061 atggcgctga cgcacagtgg cgcgcacctg ctcggctcgc tgttctgcgc cgggttcggc
     3121 tactgggtca tcgacacctt gctggattga gtgcgcgacg cgggccgccg gcttgaaaat
     3181 cggtcctgtt cccccatgtc cgtccccatc ggaggatttt tccaatgcga atcgacaagc
     3241 tgaccaccaa attccaggaa gcgctcgccg atgcccagag catcgcggtg accaacgaca
     3301 acccgtacat cgagccggtc catgtgctgg ccgcgatgct cgcgcagacc gacggcccga
     3361 aggcgctgct cgaccgcgcc ggcgtcaaca gcgcggcgct gaagaccgcg ctcgacgtgg
     3421 ccctgaaggg cctgccgcag gtgcagggca gcggccaggt cgtgcagccg agccgcgatc
     3481 tcgcggcgct gctgcagacg gccgagaagg aagcgagcaa gcgcggcgac cagttcatcg
     3541 cgagcgagat gttcctgctc gcgctggccg acgccaagac cgacctcggc ggcatcgtgc
     3601 gcgccaacgg catgacccgc aaggcgctcg acgccgcgat cgaatcggtg cgcggcggcc
     3661 agaaggtcga caacgccgag gccgagggcc agcgcgaggc gctgaagaaa tacacgctcg
     3721 acctgaccga gcgcgcccgt cttggcaagc tcgacccggt gatcggccgc gacgacgaga
     3781 tccgccgcgc gatccaggtg ctgcagcgcc gcagcaagaa caacccggtg ctgatcggcg
     3841 agccgggcgt cggcaagacg gcgatcgtcg aaggcctggc gcagcgcatc gtcgcgggcg
     3901 aagtgcccga ctcgctgaag cacaagcgcg tgctcgtgct cgacatggcg ctgctgctcg
     3961 ccggcgcgaa gttccgcggc gagttcgagg agcggctcaa gggcgtgctg aaggaggttg
     4021 cgcaggacga gggccagacc atcgtcttca tcg
//
LOCUS       DASRDG010000186         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1540295, whole genome shotgun sequence.
ACCESSION   DASRDG010000186 DASRDG010000000
VERSION     DASRDG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540295"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFA35_03020"
     CDS             complement(<1..253)
                     /locus_tag="VFA35_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HZT55170.1"
                     /translation="MATLQELIAQKEVLEREIERTRHQDRSDAIAKVRALMSQYGLTA
                     ADLSGKGGAKARAGTGNKVAAKYRNAATGETWTGRGLQPK"
     gene            complement(324..599)
                     /locus_tag="VFA35_03025"
     CDS             complement(324..599)
                     /locus_tag="VFA35_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HZT55171.1"
                     /translation="MARTVQCIYLKKEAEGLDYAPYPGELGKRIYNQVSKEAWQAWMK
                     HQTMLVNENRLNLADQRARQYLARQMENFFFGAGAEQVAGYVPPPGA"
     gene            complement(620..1960)
                     /gene="argA"
                     /locus_tag="VFA35_03030"
     CDS             complement(620..1960)
                     /gene="argA"
                     /locus_tag="VFA35_03030"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HZT55172.1"
                     /translation="MSLVFPHTFVPWFRAVAPHIHAYHGKTFVVAITGELIAAGKLNS
                     FAQDLAILHAMGIKLVLVHGFRPQVNEQLRSKGHAARFSHGLRITDAVALDCAQEAAG
                     QLRFEIEAAFSQGLPNTPMANAIVRLVSGNFLTARPVGIVDGVDFLHSGVVRKVDGAA
                     IRRAIDTDAMVLLSPFGFSPTGEAFNLTMEDVATSTAIALQADKLLFITEVPGIREDL
                     ADPDSPIDTELALADAEKLLAALPRPEKPTDPAFYLQHCVRACRGGVERSHILPFAMD
                     GALLMEVFTHDGIGTMVVDEKLESLREATSDDIGGVLQLIEPFEQDGTLVKRSRTEIE
                     RDIGHYTVIEHDGVIFGCAALYPYPEAKTAEMAALTVSPQVQSQGDGERILKRVEQRA
                     KAMGLDSIFVLTTRTMHWFIKRGFASVDPDWLPEARKRKYNWDRRSQVLVKKLS"
     gene            complement(2039..>2533)
                     /locus_tag="VFA35_03035"
     CDS             complement(2039..>2533)
                     /locus_tag="VFA35_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT55173.1"
                     /translation="LAHSPEGRRVFATMSVTDNLTLGAFPRLTGARPRGDVKADLERA
                     LELFPRLKERREQLAGTLSGGEQQMLAMARAVMLNPEVVLLDEPSMGLAPILVAEVFK
                     IIARLKGEGVTMLLVEQFAAAALGVADYGYVLENGRISVHGPAAKLRDDPAVKAAYLG
                     GGGH"
BASE COUNT          377 a          861 c          832 g          463 t
ORIGIN      
        1 acttcggctg caacccgcgg ccggtccagg tttcgccggt ggccgcgttc cggtatttcg
       61 cggcgacctt gttgcccgtg ccggcccgtg ccttggcgcc gcccttgccg gagaggtcgg
      121 cggcggtcaa tccatattgg ctcatcaagg cgcgcacctt cgcgatcgca tcgctgcggt
      181 cttgatggcg cgtgcgttcg atttcgcgct cgagcacttc cttctgggcg atcagttcct
      241 gcaacgtggc catgagaaat cccttttcct gaaacaaaat cggctgaaac tcgctcgatt
      301 ctattcagtt gccgccacga tattcaagcc ccgggcggcg ggacataccc ggccacctgc
      361 tcggcgccgg cgccgaagaa gaaattctcc atctggcggg ccagatattg gcgcgcgcgc
      421 tgatcggcca gattcaggcg attctcgttg acgagcatcg tctggtgctt catccacgcc
      481 tgccaagcct ccttgctgac ctggttgtag attctcttgc cgagttcgcc ggggtagggg
      541 gcgtaatcca agccctcggc ttccttcttc aggtagatgc attgaacggt gcgggccatc
      601 ttgaatatcc tctttcagat cagctgagtt tcttgaccag cacttgcgag cgccggtccc
      661 agttgtattt tcgcttgcgc gcctcgggca gccagtcggg gtcgaccgac gcgaaaccgc
      721 gcttgatgaa ccagtgcatc gtgcgggtcg tcaacacgaa gatgctgtcc agccccatcg
      781 ccttggcgcg ctgctcgacg cgcttgagaa tgcgctcgcc atcgccttgg ctctgcactt
      841 gcggcgatac ggtgagcgcc gccatctcgg cagtcttggc ctcggggtac gggtacagcg
      901 ccgcgcagcc gaagatcacg ccgtcgtgct cgatcacggt gtagtggccg atgtcgcgct
      961 cgatctcggt gcggctgcgc ttgacgagcg tgccgtcctg ctcgaacggt tcgatcagct
     1021 gcagcacgcc gccgatgtcg tcggacgtgg cctcgcgcag gctctcgagc ttttcgtcga
     1081 ccaccatcgt gccgatgcca tcgtgcgtga acacctccat cagcagcgcg ccgtccatcg
     1141 cgaacggcag gatgtggctg cgctcgacgc cgccgcggca ggccctgacg caatgctgca
     1201 ggtagaaagc cgggtcggtc ggcttctcgg gccgcggcag cgcggccagc agcttctcgg
     1261 cgtcggccag cgcgagctcg gtgtcgatcg ggctgtccgg gtcggccaga tcctcgcgga
     1321 tgccgggcac ttcggtgatg aacagcagct tgtcggcctg cagcgcgatc gcggtgctgg
     1381 tggccacgtc ttccatcgtc aggttgaaag cctcaccggt gggtgagaag ccgaacggcg
     1441 acagcagcac catcgcgtcg gtgtcgatcg cgcgccggat cgccgcgcca tccaccttgc
     1501 gcaccacgcc gctgtggagg aagtcgacgc cgtcgacgat gccgaccggc cgcgcggtca
     1561 ggaagttgcc cgacaccagg cgcacgatcg cgttcgccat cggcgtgttc ggcaggccct
     1621 gcgagaacgc ggcctcgatc tcgaaccgga gctggccggc cgcctcctgc gcgcaatcca
     1681 gcgcgaccgc atcggtgatg cgcaggccgt ggctgaagcg cgccgcgtgg cccttcgagc
     1741 gcagctgctc gttcacctgc ggccgaaagc cgtgcaccag caccagcttg atgcccatcg
     1801 cgtgcaggat cgccaggtct tgcgcgaacg aattcagctt gcccgcggcg atcagctcgc
     1861 cggtgatcgc gaccacgaag gtcttgccgt ggtacgcgtg gatgtgcggc gccacggcgc
     1921 gaaaccaggg cacgaaagtg tggggaaaga cgaggctcac ggcgcttgga ttcactcggt
     1981 ggggcggcga ccgcgattct gtcacgtggt gacgctgcgc tcggcggcag ctcgcgcgtc
     2041 agtgcccgcc gccgcccagg tacgccgcct tcaccgccgg gtcgtcgcgc agtttcgccg
     2101 ccgggccgtg taccgagatg cggccgttct cgagcacgta gccgtagtcg gccacgccca
     2161 gcgccgcggc cgcgaactgc tcgaccagca gcatcgtcac gccttcgccc ttgagccgcg
     2221 cgatgatctt gaagacctcg gccaccagga tcggcgcgag gcccatcgac ggttcgtcga
     2281 gcagcacgac ctcggggttc agcatcaccg cgcgggccat cgcgagcatc tgctgctcgc
     2341 cgcccgagag cgtgccggcg agctgctcgc gccgctcttt caagcgcggg aagagttcga
     2401 gcgcgcgctc caggtcggcc ttgacgtcgc ccctcggccg cgcgccggtc agccgcggga
     2461 acgcgcccag tgtcaggttg tcggtgacgc tcatcgtcgc gaacacgcgc cggccctcgg
     2521 gcgagtgcgc gag
//
LOCUS       DASRDG010000187         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1289702, whole genome shotgun sequence.
ACCESSION   DASRDG010000187 DASRDG010000000
VERSION     DASRDG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289702"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFA35_03040"
     CDS             complement(<1..210)
                     /locus_tag="VFA35_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HZT55174.1"
                     /translation="MSEPAPPPRTVPSALGAASGPAAGRPSVIQLVFREKGALYAAYM
                     PVFTDGGLFVPTTREYKLGDDIYLLL"
     gene            complement(207..1271)
                     /gene="holB"
                     /locus_tag="VFA35_03045"
     CDS             complement(207..1271)
                     /gene="holB"
                     /locus_tag="VFA35_03045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZT55175.1"
                     /translation="MVGADAQLPLPWLAPLLRQTLASQRAHALLLHGPQGVGQFEFAL
                     TLAQAWLCEVAEVGADAGTRPCGVCASCRLVQAHAHPDMLVLLPEALQEPLGWAADDE
                     AAAERASKTKAPSKEIRVEAVRAAVNFAQTTSARGRVKVVVLHPAERMNAVSANTLLK
                     TLEEPPGNARFVLSCAAPDALLPTVRSRCQSVPMALPPIEQATAWLAGQGVADPAVML
                     AAAGGQPQEALAWTQQGIDAALWLRLPALVGQGAATAFAAWPLARTVDALQKLCHDAA
                     CIAAGAAPRYFPAASFRGGAEPAALTAWMRELNRVARHAEHPWNAGLMAESLVQQGQR
                     ALAGAVGATPRRHSSVHSRP"
     gene            complement(1265..1879)
                     /gene="tmk"
                     /locus_tag="VFA35_03050"
     CDS             complement(1265..1879)
                     /gene="tmk"
                     /locus_tag="VFA35_03050"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZT55176.1"
                     /translation="MTTGRFITFEGIDGAGKSTHLSAVVERLRAAGARVVSTREPGGT
                     PLAERVREIFLHESTDVLTEALLAFAARRDHIRTVIAPALAAGDTVVCDRFTDASFAY
                     QGGGGGAAPAQLEQLEAWVQQGLQPDLTLWFDLPADIAAQRRAQARAADRFEQRDLAF
                     FERVRAGYAARAHAAPQRFARIDAALDRDAVGRQIDTALRARGW"
     gene            complement(1888..2883)
                     /gene="mltG"
                     /locus_tag="VFA35_03055"
     CDS             complement(1888..2883)
                     /gene="mltG"
                     /locus_tag="VFA35_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZT55177.1"
                     /translation="MRALRALLILLLLLIVSIGGAAFWWLHQPLELATDPVEVSVELG
                     TPPREIAQQWVQAGVQASPTLLYEWFRWSGLARKIRAGSYEIGRGVTPIALLNKMVRG
                     DETLAVVRLIDGWTFRQFRAELAKAEALKPTTALMTDAQIMAALGAPPDQSPEGRFHP
                     DSYAYSKGASDIAVLKRAYHAMQTRLEAAWQERAPDTPLHSADDALVLASIVEKETGV
                     GADRGKVAGVFVNRLRLGMPLQTDPTVIYGLGTAFDGNLRKRDLLADTPYNTYTRSGL
                     PPTPIAMPGKAALLAAVRPDPTKALYFVARGDGSSEFSETLADHNRAVNQFQRKK"
     gene            2940..>3515
                     /locus_tag="VFA35_03060"
     CDS             2940..>3515
                     /locus_tag="VFA35_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HZT55178.1"
                     /translation="MTTPLASSATDGIAPLTHLGLIRARGADAVKFLQGQLTNDVAGM
                     GLARARLAGFCSAKGRLQASFIVWKLADDELLLACAASVLPAVLKRLSMFVLRAQCKL
                     SDASAELKLLGAAGARATALVGDAPAWGVHATPGGTLVRLPDVDGIARALLAIAPADV
                     PVADLPAATWRLLDVRSGVPMIEAATVEQFVP"
BASE COUNT          455 a         1259 c         1306 g          495 t
ORIGIN      
        1 gagcagcagg tagatgtcgt cgccgagctt gtattcgcgc gtcgtcggca cgaacagccc
       61 gccatcggtg aacaccggca tgtacgcggc gtagagcgcg cccttctcgc ggaacaccag
      121 ctggatcacg ctcggccgcc cggcggccgg cccgctggca gcgcccaagg cggagggaac
      181 ggtgcgcggc ggcggtgcgg gttcgctcat gggcgcgagt gtaccgaaga gtgccgccgc
      241 ggcgtggccc cgacggcgcc ggcgagcgcg cgctggcctt gctgcacgag cgactcggcc
      301 atcagccccg cgttccacgg gtgctcggcg tgccgcgcga cgcggttcag ctcgcgcatc
      361 caggccgtca gcgcggccgg ctcggcgccg ccacggaacg atgcggctgg gaagtagcgc
      421 ggcgccgcgc ccgccgcgat gcaggccgcg tcgtggcaga gcttttgcag cgcgtcgacg
      481 gtacgcgcca gcggccaggc ggcaaacgcc gtggcagcgc cctgcccgac cagcgccggc
      541 aggcgcagcc acagggccgc gtcgatgccc tgctgcgtcc acgcgagcgc ctcctgcggc
      601 tggccgccgg ccgcggcgag catcaccgcc gggtcggcga cgccctgccc cgcgagccag
      661 gcggtggcct gctcgatcgg cggcaaggcc atcggcacgc tctggcagcg gctgcgcacg
      721 gtgggcagca gcgcgtcggg cgcggcgcaa ctcagcacga agcgcgcgtt gccgggtggc
      781 tcttcgagcg tcttcagcag cgtgttggcc gagaccgcgt tcatgcgctc ggcggggtgc
      841 agcacgacga ccttgacgcg gccgcgcgcc gaggtcgtct gcgcgaagtt cacggccgcg
      901 cgcaccgcct cgacccggat ctccttgctc ggcgccttgg tcttgctcgc gcgctcggcg
      961 gcggcctcgt cgtcggcggc ccagccgagc ggctcctgca gcgcttcggg cagcagcacc
     1021 agcatgtcgg ggtgcgcatg cgcctgcacg agccggcagc tggcgcagac gccgcagggg
     1081 cgcgtgccgg catcggcccc gacctcggcc acctcgcaca gccaggcctg cgcgagcgtg
     1141 agcgcgaatt cgaactggcc gacgccctgc ggcccgtgca gcagcagcgc atgggcgcgt
     1201 tgcgacgcca gggtctggcg cagcagtggc gccagccacg gcagcggcaa ctgggcgtcg
     1261 gcgcctacca cccgcgcgcc ctcagggcgg tgtcgatctg gcgcccgacc gcgtcgcggt
     1321 cgagcgcggc gtcgatgcgc gcgaagcgct gcggcgcggc gtgtgcgcgc gcggcgtagc
     1381 cggcgcgcac gcgctcgaag aacgcgaggt cgcgctgctc gaagcgatcg gccgcgcggg
     1441 cctgcgcgcg gcgttgcgcg gcgatgtctg cgggcaggtc gaaccagagc gtcaggtcgg
     1501 gctgcaggcc ttgctgcacc cacgcttcga gctgctcgag ctgcgccggc gccgcgccgc
     1561 caccgccgcc ctggtaggca aagcttgcgt cggtgaagcg gtcgcagacc accgtgtcgc
     1621 cggccgcaag cgcgggtgcg atcacggtgc ggatgtggtc gcggcgcgcg gcgaacgcga
     1681 gcagcgcttc ggtcagtacg tcggtcgatt cgtgcaggaa gatctcgcgc acgcgctcgg
     1741 ccagcggtgt gccgccgggc tcgcgcgtgc tcacgacacg cgcaccggcg gcgcgcagcc
     1801 gctcgaccac ggccgagagg tgggtcgact tgccggcgcc gtcgatgccc tcgaaggtga
     1861 tgaatcgacc ggtcgtcatc gctggtgtca cttcttgcgc tggaactggt tcaccgcccg
     1921 attgtggtcg gccagcgtct cgctgaactc gctgctgccg tcgccgcgcg cgacgaagta
     1981 cagcgccttg gtcgggtcgg gccgcaccgc ggccagcagc gcggccttgc ccggcatcgc
     2041 gatcggcgtc ggcggcaggc cgctgcgggt gtaggtgttg taaggcgtgt ccgcgagcag
     2101 gtcgcgcttg cgcaggttgc cgtcgaaggc cgtgcccagg ccgtagatca cggtcgggtc
     2161 ggtctgcagc ggcatgccca ggcgcaggcg gttgacgaac acgccggcga ccttgccgcg
     2221 gtcggcgccg acgccggtct ccttctcgac gatcgaggcc agcaccagcg cgtcgtcggc
     2281 gctgtgcagc ggcgtgtcgg gcgcgcgctc ctgccaggcg gcttcgaggc gcgtctgcat
     2341 ggcatggtag gcgcgcttga gcacggcgat gtcgctcgcg cccttgctgt aggcgtagct
     2401 gtcgggatgg aagcgccctt ccggcgactg gtccggcggc gcgccgaggg cggccatgat
     2461 ctgcgcgtcg gtcatcaaag cggtggtggg cttgagcgcc tcggccttcg cgagctcggc
     2521 gcggaactgg cggaaggtcc agccgtcgat caggcgcacg accgcgaggg tttcgtcgcc
     2581 gcgcaccatc ttgttgagca gcgcgatcgg ggtcacgccg cggccgatct cgtagctgcc
     2641 cgcgcggatt ttgcgcgcca gccccgacca gcggaaccat tcgtacagca aggtcggcga
     2701 ggcctgcacg ccggcctgca cccactgctg cgcgatctcg cgcggcggcg tgccgagctc
     2761 gaccgagacc tcgaccggat cggtcgccag ttcgagcggc tggtgcagcc accagaacgc
     2821 cgcgccgccg atcgagacga tcagcagcaa cagcaggatc aacaacgcac gcagggctct
     2881 catcagacca accgaaaccc cgagggcacg agaaacacgg gcgctgatga taatcgggca
     2941 tgaccacgcc cctcgcctca tccgccaccg acggcatcgc gccgctcacc cacctcggcc
     3001 tgatccgcgc gcgcggcgcc gacgccgtga agttcctgca gggccaactc accaacgatg
     3061 tcgccggcat gggcctcgcg cgcgcgcgcc tggcgggctt ttgttccgcc aagggccgct
     3121 tgcaggcgag cttcatcgtc tggaagctgg ccgacgacga gttgctgctc gcctgtgccg
     3181 cgagtgtgct gccggccgtg ctgaagcggc tgtcgatgtt cgtgttgcgc gcacagtgca
     3241 agctcagcga tgcgagcgcc gagttgaagc tgctcggtgc cgccggcgcg cgcgccacgg
     3301 cgttggtcgg cgacgcgccc gcctggggcg tgcacgccac gcccggcggc acgctggtgc
     3361 gcctgcccga tgtcgacggc atcgcgcgcg cgctgctcgc gatcgcaccg gctgatgtgc
     3421 cggtggccga tctgcccgcc gccacctggc ggctgctcga tgtgcgctcg ggcgtcccga
     3481 tgatcgaggc cgccacggtc gagcagttcg tgccg
//
LOCUS       DASRDG010000188         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_831493, whole genome shotgun sequence.
ACCESSION   DASRDG010000188 DASRDG010000000
VERSION     DASRDG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831493"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /gene="aceK"
                     /locus_tag="VFA35_03065"
     CDS             complement(<1..1754)
                     /gene="aceK"
                     /locus_tag="VFA35_03065"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HZT55179.1"
                     /translation="MTLAATFPTALSDPRAFDIAQAMLDGFDRHYTLFRETSAAAKRR
                     FEAADWHGQQRAQRERIEFYDLRVDEAAERLQTEFQASELPKDVWQQVKLHYIGLLTG
                     HLQPELAETFFNSVTTKILHRSYFNNDFIFMRPAVSTEYIENDEPASLPTYRAYYPTK
                     ATMRETWRRIVDDFALASPFEDLGRDIDDVLAAVDAQLGEYRMRANFQIQVLSSLFYR
                     NKGAYLVGKIINGFNETPFALPILHSERPAPGRPKAAERPSGADAAGVLGLHDTGLVI
                     DTVLFGEDELLRLFSFARAYFMVAMAVPSAYVQFLRSMMPRKPRSEIYSALGLAKQGK
                     NAFYRDFLHHLKHSSDRFRIAPGIKGMVMLVFDLPSYPYVFKVIKDFYPPPKDTTREL
                     IKSKYLLVKQHDRVGRMADTLEYSNVAFPRARFEDELVAELKHFCASLLEEDGDALVI
                     RHVYMERRMIPLNIYLQEASLAQIESVVVEYGNAIKDLLRANIFPGDMLWKNFGVTRD
                     GKVVFYDYDEIEYLTDCNVRKVPPPRYEEDEMSGEPWYTVGPHDIFPETYNTFLLGDP
                     RVREAFMRHHADFFDPAL"
     gene            complement(1759..2499)
                     /gene="can"
                     /locus_tag="VFA35_03070"
     CDS             complement(1759..2499)
                     /gene="can"
                     /locus_tag="VFA35_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HZT55180.1"
                     /translation="MTTQLQELLDNNRLWAAKTEQRSPGFFTGLLAQQAPQYLWIGCA
                     DSRVPANELVDLLPGELFVHRNVANVVVHSDLNCLSVIQFATDLLRVKHIIVVGHARC
                     GGVNAALNDIRVGLADNWIRHVQDVRNRHREWLDTLPAEEQTDALGELNVIEQARNVC
                     QTTVVQDAWARGQELVVHGWFYGLNNGLLRDLKFTVSNAADMADAFVAAIDSLHGRHA
                     LKTASVMGSLGGPELAPVPAGGGLAETP"
     gene            2634..>3289
                     /locus_tag="VFA35_03075"
     CDS             2634..>3289
                     /locus_tag="VFA35_03075"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZT55181.1"
                     /translation="MSFDGSAIKALLDGAVSKGAVHGIAAVVVDRSGPLFHHAAGEAG
                     ERTMFRNASMTKAVATTAALQLVEQGVLSLDAAVETILPEFGELQVLDGFDGDTPRLR
                     PPASQATVRQLMTHSAGLGYFFLNEKLLRYHQLTGEPNPLSGLKRSLSVPLVNDPGTV
                     WEYGVNTDWLGLIVEKLSGQTLGSYLAQNVYGPLGMNDSTFAPSAEQRGRLLRVMQRQ
                     A"
BASE COUNT          535 a         1046 c         1143 g          565 t
ORIGIN      
        1 cacagcgcgg gatcgaagaa atcggcgtga tggcgcatga aggcctcgcg cacacgcggg
       61 tcgccgagca ggaacgtatt gtaggtttcc gggaagatgt cgtgagggcc gacggtatac
      121 cacggctcgc ccgacatctc gtcttcctcg tagcgcggcg gcggcacctt gcgcacgttg
      181 caatcagtga ggtactcgat ctcgtcgtag tcgtagaaca cgaccttgcc gtcgcgcgtg
      241 acgccgaagt tcttccagag catgtcgccg gggaagatgt tggcgcgcag caggtccttg
      301 atcgcgttgc cgtattcgac caccaccgac tcgatctgcg cgagcgaggc ctcctgcagg
      361 tagatgttga gcgggatcat gcgccgctcc atgtagacgt ggcggatcac cagcgcgtcg
      421 ccgtcttcct cgagcaggct ggcgcagaag tgcttcagtt cggcgacgag ctcgtcctcg
      481 aagcgcgcgc gcggaaacgc gacgttgctg tactcgagcg tgtcggccat gcggccgaca
      541 cggtcgtgct gcttgacgag caggtacttg ctcttgatca gctcgcgcgt cgtgtccttg
      601 ggcggcgggt agaagtcctt gatgaccttg aagacgtacg gatagctcgg caggtcgaac
      661 accagcatca ccatgccctt gatgccgggc gcgatgcgga agcggtcgct cgaatgcttc
      721 aggtggtgga gaaagtcgcg gtagaacgcg ttcttgccct gcttcgcgag gccgagcgcg
      781 ctgtagatct cgctgcgcgg cttgcgcggc atcatcgagc gcaggaactg cacgtaggcc
      841 gacggcaccg ccatcgccac catgaagtag gcgcgcgcga agctgaacaa gcgcagcaac
      901 tcgtcctcgc cgaacagcac cgtgtcgatc accagcccag tgtcatggag ccccaagacg
      961 cctgcggcgt cggcccccga ggggcgttcg gccgccttgg ggcggcccgg cgccggcctc
     1021 tctgaatgga ggatcggcag cgcgaacggc gtctcgttga agccgttgat gatcttgccg
     1081 accaggtacg cgcccttgtt ccggtagaac agcgaactca gcacctggat ctggaagttc
     1141 gcgcgcatcc ggtactcgcc gagctgcgcg tcgaccgcgg cgagcacgtc gtcgatgtcg
     1201 cggccgaggt cttcgaacgg gctcgcgagc gcgaagtcgt cgacgatgcg ccgccaggtc
     1261 tcgcgcatcg tcgccttggt cgggtagtag gcgcggtagg tcggcagcga agcgggctcg
     1321 tcgttctcga tgtactcggt cgagaccgcc gggcgcatga agatgaagtc gttgttgaaa
     1381 tagctgcggt gcaggatctt cgtcgtcacc gagttgaaga aggtctcggc gagttcgggc
     1441 tgcaggtggc cggtgagcag gccgatgtag tgcagcttga cctgctgcca cacgtccttc
     1501 ggcagctcgg acgcctggaa ctcggtctgc aaccgctcgg ccgcctcgtc gacgcgcagg
     1561 tcgtagaact cgatgcgctc gcgctgggcg cgctgctggc cgtgccagtc ggccgcctcg
     1621 aagcggcgct tcgcggcggc gctggtctcg cggaacagcg tgtagtggcg gtcgaagcca
     1681 tcgagcatgg cctgcgcgat gtcgaacgcg cgcgggtcgc tcagcgcggt cgggaacgtc
     1741 gcggcgagcg tcatggcctc atggcgtctc ggcaagcccc ccgccggcag gcaccggggc
     1801 gagttcgggg ccgcccagcg agcccatcac cgaggcggtc ttcagcgcat ggcgcccgtg
     1861 caacgaatcg atcgccgcga cgaacgcgtc ggccatgtcg gcggcgttcg agaccgtgaa
     1921 cttcaggtcg cgcagcaggc cgttgttcag gccgtagaac cagccgtgca cgaccagctc
     1981 ctggccgcgc gcccaggcgt cctgaacgac cgtggtctgg cagacgttgc gcgcctgctc
     2041 gatcacgttg agctcgccga gcgcgtcggt ctgctcctcg gccggcaaag tatcgagcca
     2101 ttcccggtgg cggttgcgca cgtcctgcac gtggcggatc cagttgtcgg ccaggccgac
     2161 acggatgtcg ttcagcgccg cgttcacgcc gccgcagcgc gcgtggccga cgacgatgat
     2221 gtgcttgacg cgcaaaaggt cggtcgcgaa ctggatcacc gacaggcagt tcaggtcgga
     2281 gtgcaccacc acgttcgcga cgttgcggtg cacgaagagc tcgcccggca gcaggtcgac
     2341 cagttcgttc gccggcacgc ggctgtcggc gcagccgatc cacaggtact gcggcgcctg
     2401 ctgggcgagc agcccggtga agaagccggg gctgcgctgc tcggtcttcg cggcccatag
     2461 gcggttgttg tcgaggagct cttgaagctg ggtggtcatg gtgcggtgcg gcagagttgc
     2521 cggaagtgta cgcatcgcag aagccccgca tccggccgcc gcgcgcagaa tgacacgacc
     2581 gtaccggagg cgatctcgcg caccggtgcc aaccctgaac gaaggagatg accatgtcct
     2641 ttgacggttc ggccatcaag gcgctgctcg acggtgcggt ttcgaaaggt gccgtgcacg
     2701 gcattgcggc ggtcgtcgtc gaccgcagcg gccccctctt ccaccacgcc gccggcgagg
     2761 ccggcgagcg cacgatgttc cgcaatgcct cgatgaccaa ggcggtggcc accacggcgg
     2821 cgctgcaact cgtggaacaa ggcgtgctga gcctcgatgc cgccgtcgag acgatcctgc
     2881 ccgagttcgg cgaattgcag gtgctggacg gtttcgacgg cgacacgccg cgcctgcgcc
     2941 ctcctgcgag ccaggctact gtgcgccagc tgatgacgca ctcggccggc ctgggctact
     3001 tcttcctcaa cgagaagttg ctgcgctatc accagctgac gggtgaaccc aacccgctgt
     3061 cgggtctgaa gcgcagcctg tcggtgccgc tcgtcaacga cccggggacg gtgtgggagt
     3121 acggcgtcaa caccgactgg ctcgggctca ttgtcgagaa gctgtcgggg cagaccttgg
     3181 gcagctacct cgcgcagaac gtgtatgggc cgttggggat gaacgactcg acgttcgcgc
     3241 ccagcgccga gcagcggggc cggctgctgc gcgtgatgca gcgccaggc
//
LOCUS       DASRDG010000189         5604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_260144, whole genome shotgun sequence.
ACCESSION   DASRDG010000189 DASRDG010000000
VERSION     DASRDG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260144"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..589
                     /locus_tag="VFA35_03080"
     CDS             188..589
                     /locus_tag="VFA35_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HZT55182.1"
                     /translation="MATAKKAPAKKAAPAKKAAPAAKKAAPAKKAAAKKAAPAKAAAK
                     KAAPAKKRTPNAAFMKAMTPSAALAAIIGAKPVPRTEVTKKVWEYIKANKLQDAAKKT
                     MINADAKLKEIFKKAQVSMFEMTKLINAHLS"
     gene            complement(649..1170)
                     /gene="pal"
                     /locus_tag="VFA35_03085"
     CDS             complement(649..1170)
                     /gene="pal"
                     /locus_tag="VFA35_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZT55183.1"
                     /translation="MIKSLAITLVAAAALAACSSTPLDTPKAPVATATLPPAGGASTA
                     QSTVATVTLDPLDDPKSNLAARSVYFAYDDFTVAPKYQPLVQAHGQFLQSHAQVQIRV
                     EGNTDERGSREYNLALGDKRAQIVAKQLELMGAKAAQIEAVSYGEERPKAEGHDEAAW
                     AENRRADIRYVKR"
     gene            complement(1187..1702)
                     /locus_tag="VFA35_03090"
     CDS             complement(1187..1702)
                     /locus_tag="VFA35_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZT55184.1"
                     /translation="MKMDASPASLDVLQQLRVVVRLAGNHSARVERRTGIPGAQLWAL
                     HEIALADGLRVGELAQRLRVHQTTVSNLLSRLDAAALVRKGRSRHDQRIVHVHLTAAG
                     RKALKAAAAPARGLLLSVLDGMSAAQLRKVHDGLAVLVDHMGGFDAALAAKPLPFTES
                     TGRSSRLSSRS"
     gene            1836..3014
                     /locus_tag="VFA35_03095"
     CDS             1836..3014
                     /locus_tag="VFA35_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZT55185.1"
                     /translation="MDMELLGPLVAWPSMLLAAWLAGEWVFRRWNLPRVCAYAVVGLV
                     LGGTGLSQNLASHAAQGFMANVALALTLFELGYRINPRWFRHNPWVLAAGIAQAVLTF
                     GAVFWVAGVFALPIDHRLVLAALCVASSPAAIMRVTHELGSAGQVTERLLHLCALNCL
                     VAVLLLRLVVGYWHLETSGELGKAAFNSIYVIGLSLVAGAAFGVVAPLLNRIATSSQS
                     TTVTFALLVLLLTGLAQSLHLSPLLAALAFGVVARERRIMLSHAQRNFGALGDVLSVF
                     LFVFVGSLLSWSGLLASLLLGIAVLLTRTASHTLVNLSLAHVSGTTFRKGALTGLALM
                     PMAAFALLLLEESRLYGFDLARDSLPAIVGLLVLLDVLGPLATQRALMLAGESHPEGG
                     "
     gene            3017..4147
                     /locus_tag="VFA35_03100"
     CDS             3017..4147
                     /locus_tag="VFA35_03100"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HZT55186.1"
                     /translation="MPLGEFGKSQALSLGVELELQLVNTVDYDLTSAANDLLELLSRK
                     PFPGTVTPEMTQSMIEIATGIQTEHAPLLAELRGMRDTLAAACDRLNIGIAGGGTHPF
                     QRWSEQRVFDRPRYQEISQLYGYLAKQFTVFGQHVHVGCASGDDALFLLHSLSRYVPH
                     FIALSASSPFFQGVDTLFDSARLNSVFAFPLSGRAPFVLRWDEFANGYFTKMEATGVV
                     KSMKDFYWDIRPKPEYGTIELRVCDTPLTVERAAALACYLQAICRRLLERVEPMPTED
                     DYLVYTFNRFQACRFGLDGTMVDPRTRESRTIREDILATLRHVEPHGAVLQSLPALEE
                     IERCAALDGNHATYLRKVFDETRNVQSMVQESARALRAGPPC"
     gene            complement(4158..4739)
                     /locus_tag="VFA35_03105"
     CDS             complement(4158..4739)
                     /locus_tag="VFA35_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZT55187.1"
                     /translation="MHPIVSRPAADRARVRTLRLAFAAGLAVAAGLGVPRPAHPEAAT
                     RCNTTLSPEAVLQRLNATRSRGAVCHRPGTPTSAAPLAWNARLAEVAAAQAQDMARLD
                     HMSHRDSRNRGLGERLDAMGYRFSAAAENVAVGYASLDAVVDAWLDSEAHCVNLMNAK
                     VAELGLACSDGSANDSGTGRYWTLVLGAPSRSR"
     gene            4944..>5604
                     /locus_tag="VFA35_03110"
     CDS             4944..>5604
                     /locus_tag="VFA35_03110"
                     /inference="COORDINATES: protein motif:HMM:NF038119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHFG family PEP-CTERM protein"
                     /protein_id="HZT55188.1"
                     /translation="MKRSHLWVIAVLLGSIGAAHASEAVPPTLTLDHCSWDRPGVNPF
                     MGDVVAAVDRYRDIPPDARARLKDRMAKREYDDLVEIRRDTMHGRGGYEYGSAIRDMH
                     FGTHQVCRSVTRASWTPQMQERGLVYCESGHCILVPTVCRNVSRVTRRGVGPETLAGP
                     PLLGGDPADLALAPLPLMADAPEALAPVGGDGPLARPLEGGPIGHVDGPGGIVGPAWW
                     SD"
BASE COUNT          832 a         1907 c         1965 g          900 t
ORIGIN      
        1 ggtgacgcct ggccacgaca gagcgctgtc tacctcggag aattgcgcgt ttctccagtg
       61 aaatcttctt ctctttgccc tatgcgggca ttaccattcg ctccgcacgg tgctatggag
      121 aaatgagagt ccacgcggct ctcgcccatc ccgggcacat caacaactct tgatggagag
      181 aatcaacatg gcaactgcga agaaggcgcc cgcgaaaaaa gcggcgccgg cgaagaaggc
      241 tgctccggca gcgaagaaag cggcacctgc gaagaaggcc gcggccaaga aggcagcacc
      301 cgcgaaggcg gctgccaaga aggcggctcc ggccaagaag cgcactccca acgcggcctt
      361 catgaaggcc atgaccccga gcgccgcact cgccgcgatc atcggcgcca agccggtgcc
      421 gcgcaccgaa gtgacgaaga aggtctggga gtacatcaag gccaacaagc tgcaagacgc
      481 ggcgaagaag acgatgatca acgccgacgc caagctgaag gaaatcttca agaaggcgca
      541 agtctcgatg ttcgagatga ccaagctgat caacgctcac ctgagctgat cgcgcttcgc
      601 gcgtggaagg gccggtgctt gcaccggcct ttttcgtttc cgcgcgggtc agcgcttcac
      661 gtagcggatg tcggcgcggc ggttctcggc ccaggcggct tcgtcgtggc cctcggcctt
      721 cgggcgctcc tcgccgtagc tgacggcctc gatctgcgcc gccttggcgc ccatcagctc
      781 gagctgcttc gcgacgatct gcgcgcgctt gtcgccgagc gcgaggttgt actcgcgcga
      841 gccgcgctcg tcggtgttgc cctcgacgcg gatctgcacc tgcgcgtgcg actgcaggaa
      901 ctggccgtgc gcctgcacca gcggctggta cttcggcgcg accgtgaagt cgtcgtaggc
      961 gaagtagacg ctgcgtgcgg ccaggttgct cttcgggtcg tcgagcgggt cgagcgtgac
     1021 ggtcgcgacc gtgctctgcg cggtcgaggc gccgccggcc ggcggcagcg tggcggtcgc
     1081 gaccggcgcc ttcggcgtgt cgagcggcgt gctgctgcag gcggcgagcg cggcggcggc
     1141 gacgagggtg atggcgaggg acttgatcat gcgaggactc ctgaagtcat gaacgggaag
     1201 acaaacggga agaccgaccc gtcgattcgg tgaagggaag cggcttcgcg gccagcgccg
     1261 catcgaagcc gcccatgtgg tcgacgagca cggccaggcc gtcgtggacc ttgcgcagct
     1321 gcgcggcgct catgccgtcg agcacgctca gcagcaagcc gcgtgccggt gcggcggcgg
     1381 ccttcagcgc cttgcggccg gcggccgtca ggtggacatg gacgatgcgc tggtcgtgcc
     1441 gcgagcggcc cttgcgcacc agcgccgcgg cgtcgaggcg gctcagcagg ttgctgaccg
     1501 tggtctgatg cacgcgcagg cgctgcgcca gttcgccgac gcgcaggccg tcggcgagcg
     1561 cgatctcgtg cagggcccag agctgcgcgc ccgggatgcc ggtgcggcgc tcgacccgcg
     1621 ccgagtggtt gccggccagc cgcacgacca cgcgcaactg ctgcagcacg tccagactgg
     1681 ccggcgatgc atccattttc atttgcacaa agtatattgc tgcaaatgta ttggagttgt
     1741 ttcgggatgc gtcggcaaag gccactttcg caaggttgag atgccgcgac acgttaggct
     1801 gcgcgcccag aacaacaacg agaggggcgc cgtcgatgga catggagctg ctgggcccac
     1861 tggttgcgtg gccgtcgatg ctgctggccg cctggctggc cggggaatgg gtcttccggc
     1921 gctggaacct gccgcgcgtc tgtgcgtatg cggtggtcgg cctggtactg ggcggcaccg
     1981 gcctgtcgca gaacctggcc tcgcacgcgg cccagggctt catggcgaac gtcgcgctcg
     2041 cgctcacgct gttcgagctc ggctaccgca tcaacccgcg ctggtttcgc cacaacccgt
     2101 gggtgctggc ggccggcatc gcgcaggcgg tgctgacctt cggcgcggtg ttctgggtcg
     2161 ccggcgtctt cgcgctgccg atcgaccacc ggctcgtgct cgcggcgctg tgcgttgcga
     2221 gctcgccggc cgcgatcatg cgcgtgacgc acgagctggg cagcgccggg caggtgaccg
     2281 agcggctgct gcacctgtgc gcgctgaact gcctggtcgc ggtgctgttg ctgcggctgg
     2341 tggtcggcta ctggcacctg gagaccagcg gagagctcgg caaggcggcg ttcaacagca
     2401 tctacgtgat cggcctgtcg ctggtcgccg gcgcggcgtt cggcgtcgtc gcgccgctgc
     2461 tgaaccgcat cgcgacgagc agccagagca cgaccgtgac ctttgcgctg ctcgtgctgc
     2521 tgctgaccgg cctggcgcag agcctgcacc tctcgccgct gctcgccgcg ctcgcgttcg
     2581 gcgtggtcgc gcgcgagcgg cgcatcatgc tgagccatgc gcagcgcaat ttcggtgcac
     2641 tcggcgacgt gctctcggtg ttcctgttcg tcttcgtcgg cagcctgctg tcgtggtccg
     2701 gcctgctcgc gagcctgctg ctcggcatcg cggtgctgct cacgcgcacc gcatcgcaca
     2761 cgctggtgaa cctgagcctg gcgcacgtca gcggcacgac gtttcgcaag ggcgcgctga
     2821 ccggcctggc gctgatgccg atggcggcgt tcgcgctgct gctgctcgaa gagagccggc
     2881 tctacggctt cgacctggcg cgcgacagcc tgccggcgat cgtcggcctg ctggtgttgc
     2941 tggacgtgtt ggggccgctt gcgacgcagc gggcgctgat gctcgccggc gagtctcacc
     3001 ccgagggagg ttagccatgc cgctgggaga attcggaaag tcacaggcgc tgtcgctcgg
     3061 cgtcgagctc gaactgcagc tcgtcaacac cgtcgactac gacctgacct cggccgcgaa
     3121 cgacctgctg gagctgctct cgcgcaagcc cttcccgggc accgtcacgc ccgagatgac
     3181 gcaaagcatg atcgagatcg cgaccggcat ccagaccgag cacgcgccgc tgctggccga
     3241 gttgcgcggc atgcgcgaca cgctggcggc ggcgtgcgac cggctcaaca tcggcatcgc
     3301 cggcggcggc acgcacccgt tccagcgctg gagcgagcag cgcgtgttcg accggccgcg
     3361 ctaccaggag atttcgcagc tttacggcta cctcgccaag cagttcaccg tgttcggcca
     3421 gcacgtgcac gtgggctgcg cgtcgggcga cgacgcgctg ttcctgctgc attcgttgtc
     3481 gcgctacgtg ccgcacttca tcgcgctgtc ggcttcgtcg ccgttcttcc agggcgtcga
     3541 cacgttgttc gattcggcgc ggctgaactc ggtgttcgcg ttcccgctca gcggccgcgc
     3601 gccgttcgtg ctgcgctggg acgagttcgc gaacggctac ttcacgaaga tggaagcgac
     3661 cggcgtcgtc aagtcgatga aggacttcta ctgggatatc cgccccaagc ccgagtacgg
     3721 cacgatcgag ctgcgcgtgt gcgacacgcc gctcaccgtc gagcgcgccg ccgcgctcgc
     3781 gtgctacctg caggcgatct gccggcgcct gctcgagcgc gtcgagccga tgccgaccga
     3841 agacgactac ctcgtctaca ccttcaaccg cttccaggcc tgccgcttcg gcctggacgg
     3901 cacgatggtc gacccgcgca cgcgcgagag ccgcacgatc cgcgaagaca tcctcgccac
     3961 gctgcgccac gtcgagccgc acggcgcggt gctgcaaagc ctgcccgcgc tcgaagagat
     4021 cgagcgctgc gccgcgctcg acggcaacca tgcgacctac ttgcgcaagg tattcgacga
     4081 gacccgcaat gtgcagagca tggtgcagga gagcgcgcgc gcgctgcgcg ccgggccgcc
     4141 gtgttgacgc gccgcgctca gcgcgagcgc gacggcgcgc ccagcaccag ggtccagtag
     4201 cggcccgtgc cgctgtcgtt ggcgctgccg tcgctgcacg ccagaccgag ttcggcgacc
     4261 ttcgcgttca tcaggttgac gcagtgggcc tcgctgtcga gccaggcgtc cacgaccgcg
     4321 tcgagcgacg cgtagccgac cgcgacgttc tcggcggctg cgctgaagcg gtagcccatc
     4381 gcgtcgaggc gctcgccgag gccgcggttg cggctgtcgc gatggctcat gtgatcgaga
     4441 cgcgccatgt cctgcgcctg cgcggcggcg acctccgcca ggcgcgcatt ccaggcgagc
     4501 ggcgccgcgc tggtcggcgt acccggtcgg tgacagaccg cgccgcggct gcgcgtcgcg
     4561 ttcaggcgct gcagcacggc ctcgggcgac agcgtggtgt tgcagcgcgt ggccgcctcc
     4621 ggatgggccg ggcgcggcac gcccagcccg gccgccacgg ccaggcccgc ggcgaaggcg
     4681 aggcgcaggg tgcgaacacg ggcgcgatcg gcggcagggc gcgaaacaat tggatgcatg
     4741 gcgggacatc ggagcgaacg acacccgtct tgagcaaaaa cgacgccggt gtcgccttcc
     4801 agatgccatc ggcgcgcgcg ggcgccgccc aacgtgactt attgactccc ccgacccaag
     4861 gtcaaccccc gtaagcccgg ggcagagcca ctggcgtgcc gcgctacatt ccctttcatg
     4921 ttgcaatccg ttacggaact gccatgaagc gatcgcacct ctgggtgatc gccgtgctgc
     4981 tgggcagcat cggcgcggct cacgcgagcg aggctgtgcc tccgacactc acgctcgacc
     5041 actgcagctg ggaccgaccc ggcgtgaacc cgttcatggg cgatgtcgtt gccgccgtcg
     5101 accgctatcg cgacattccg cccgacgcgc gcgcgcggct gaaggaccgc atggccaagc
     5161 gcgagtacga cgacctggtc gagattcgcc gcgacacgat gcacgggcgc ggtggctacg
     5221 aatacggtag cgcgatccgc gacatgcact tcggcacgca ccaggtctgc cgctcggtga
     5281 cgcgtgcgtc gtggacgccg cagatgcagg agcgcggcct cgtctattgc gagagcggtc
     5341 actgcatcct ggtgcccacg gtgtgccgca acgtcagccg cgtcacgcgc cgcggtgtcg
     5401 gccccgagac gctcgccggc ccgccgttgc tgggcggcga tccggccgac ctggcgctcg
     5461 ccccgctccc gctgatggcg gatgcgcccg aggcgctggc gccggtcggg ggggatggcc
     5521 cgctcgctcg cccgctcgag ggcggcccga tcggccacgt ggatggccct ggcggcatcg
     5581 tcgggcctgc gtggtggagc gatg
//
LOCUS       DASRDG010000190         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_831553, whole genome shotgun sequence.
ACCESSION   DASRDG010000190 DASRDG010000000
VERSION     DASRDG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831553"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1903
                     /locus_tag="VFA35_03115"
     CDS             <1..1903
                     /locus_tag="VFA35_03115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZT55189.1"
                     /translation="HRDIKPANMILLADGSVKLADFGVARIDRSELTQTGMVLGTPMY
                     MAPEQMLGLEVDYRADLFACGVVLYQFLTGERPFTGSVTAVMQKVLNEPVDPPSHRNS
                     ALVPAWDAVLNMALAKKPEQRFATAQAFATAIQRATLLQQGADSKVSRPSDGTTIAWP
                     VATDAPSPAPPSRPAPVAALLALAGVAVGGYWWSQSGTRVPEGPGPTPPASAVAGLAP
                     YAASATSVTTAASTRTGTVEESASSAAASVVAKSGNGNQGSIGSARPEAARTQPRAGD
                     ASRATAEPAAAPPRPRASTPVALQVKTPAPAAVALPAPPPPTTTALEWQAARSRLAEA
                     NVLRSLGEALVRLLDLRSADERQLVIGLEQWLQRRPASALVLGAGDGWLQFSWRSAMP
                     TVGEARALAMQRCREFGSAPPAQACRVVWSDGQLDRAAFDEVASGLGERSLGSVRSGF
                     LTNLGKALSQVMAQAQASAQVPVSAPPRPPPPAGTAADSTTSALPHSAWLETRHSLQQ
                     GRGRQSLAQSLGKLLQVESADDQQLLKRFEQKMQRLPWKSALAMGEGNGLLGYGFTWN
                     ESRGDHAAERALGICNRVSTQPCVIVMTNGRLLDEGLWSVAMRLGARPPSAVREALLP
                     YLRRDSNRD"
     gene            2082..>2583
                     /locus_tag="VFA35_03120"
     CDS             2082..>2583
                     /locus_tag="VFA35_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55190.1"
                     /translation="MRPAAEALSDPDTRAGTASRFGFRWFVLELLKHKHVWRDVLLAS
                     LALQLVALATPFFTQAIIDKMVVQTVRRGRQRPTLRRDRKALTLAWCKQSSEHRATVV
                     ATAQVHDAETCDYEETFVYVTSSGGFTLREVFYTVPPRLIGHRLRVRLYDDRLELLLG
                     RPCSWRW"
BASE COUNT          444 a          868 c          866 g          405 t
ORIGIN      
        1 gcatcgcgac atcaagcccg cgaacatgat cctgctggcc gacggcagcg tcaaattagc
       61 cgacttcggc gtcgcgcgca tcgaccgttc cgagctcacg caaaccggga tggtgctggg
      121 cacgccaatg tacatggcgc ccgagcagat gctgggactc gaggtcgact accgtgccga
      181 cctgttcgcc tgcggcgtgg tgctttacca gttcctcact ggtgagcgcc cctttaccgg
      241 atcggtgacg gcggtgatgc agaaggtgct caacgagcct gtcgacccgc ccagccaccg
      301 gaattctgcg ctggtgccag cctgggacgc cgtgctgaat atggcgttgg ccaaaaagcc
      361 cgagcagcgc ttcgcgacgg cgcaggcgtt tgccactgcg atccagcgcg caacattgct
      421 ccagcagggc gccgacagca aggtgtcgcg cccatccgat ggaacgacga tcgcatggcc
      481 ggtcgcaacc gacgcgccga gcccggcacc gccatcgcga ccagcgccgg tggcggcgct
      541 gctggccttg gcaggggtgg cggtcggggg atattggtgg tcgcaatccg gaactcgggt
      601 gccggaagga cccggcccga ccccgccggc cagcgctgtc gccgggttgg caccgtacgc
      661 ggcctcggcc acctccgtga cgaccgccgc gtcgacccga actgggacag tagaggaaag
      721 tgcgtcgagc gctgcggcgt cggttgtcgc caaatcaggc aacggcaacc aaggctcgat
      781 tgggtccgcc cgaccagagg cagcgagaac tcagccaagg gcgggcgatg catctcgcgc
      841 aacggcggaa ccggctgccg ccccgccccg acccagagcg tccacgcccg ttgcgctgca
      901 ggtcaaaacg ccggcacctg cagcagtggc tctgccggcg ccgccaccgc ccacgactac
      961 ggcgctcgaa tggcaagccg cacgttctcg actggccgag gcgaacgttc tgcgctccct
     1021 gggggaagcc ctcgtccgcc tgctagacct gcgcagtgcc gacgagcggc aactcgtgat
     1081 cggtctagag cagtggttac aacgccggcc ggcgagcgca ttggtactcg gtgcgggcga
     1141 cggatggctg cagttctcct ggcgctccgc gatgccgacc gtcggcgaag cgcgggcgct
     1201 ggccatgcaa cgatgccgcg aattcggcag cgccccgccg gcacaggctt gccgcgtggt
     1261 gtggtccgat ggccagttgg accgcgcagc gttcgacgaa gtggccagcg gtttgggaga
     1321 gcgcagcctg ggttcggtgc gcagcggctt cctgaccaac ctgggcaagg ccctgtcgca
     1381 ggtgatggcg caggcacaag cgtcagccca ggtcccggtc tctgcaccgc cgcgcccgcc
     1441 gcccccggcg ggcaccgcag ccgactccac caccagcgct ttgccacatt cagcatggct
     1501 cgagacgcgg cactcgctgc aacaaggtcg tggccggcaa agcttggccc agtcgctcgg
     1561 gaagctactg caggtcgagt cggctgacga ccagcagttg ttgaagcgct tcgagcaaaa
     1621 gatgcagcgc ctgccatgga aaagtgcgct ggcgatgggc gaaggcaatg gcttgctggg
     1681 gtacggtttc acctggaacg agtcgcgcgg cgaccatgcc gccgagcgcg cgctgggcat
     1741 ctgcaacagg gtcagcacgc aaccctgcgt catcgtcatg accaatggca ggctgctcga
     1801 cgaaggcctg tggtctgtcg ccatgcggct tggtgccagg ccaccgtccg cggtgagaga
     1861 ggcgttgctg ccgtacctgc gacgcgacag caaccgagac tagcagtgcg gggacaggcc
     1921 cgctccacgc tggtcgggtc caggctacct cagtctaacg agcgcttcga cgctgggcat
     1981 tggcgcggtg gccagtggca tgacgccaca cagcggcatc cgaacatccg cgcgggaatc
     2041 tctacagcgc gcgtatcaag gtctgatcat caagcgcttg ggtgcggccc gcggccgagg
     2101 cactttccga tccggacacg cgggccggca ccgcgagcag attcggcttc aggtggttcg
     2161 tgctcgaatt gctcaagcac aagcacgtct ggcgcgacgt gctgcttgcg tcgctcgcgc
     2221 tgcaactcgt cgcgctcgcc acaccgttct tcacgcaggc gattatcgac aagatggtcg
     2281 tgcaaactgt tcggcgaggg cggcaacgac caactctacg gcgagaccga aaggcgctca
     2341 cgctggcgtg gtgcaagcaa tccagcgagc atcgcgccac cgtcgtcgca accgcgcagg
     2401 ttcacgacgc cgaaacctgc gactacgagg agacgttcgt ctacgttacc tcgtccggtg
     2461 gcttcacgct gcgcgaggtc ttctacaccg tgccgccccg gctgatcggc caccgcttgc
     2521 gggttcgcct gtacgacgac cggctcgaac tgctgctggg gcgaccttgc tcatggcgct
     2581 gga
//
LOCUS       DASRDG010000191         6824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_484295, whole genome shotgun sequence.
ACCESSION   DASRDG010000191 DASRDG010000000
VERSION     DASRDG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484295"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFA35_03125"
     CDS             complement(<1..372)
                     /locus_tag="VFA35_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZT55191.1"
                     /translation="MATQIEIAQRVVESILAQKLKPGERLGEQELADMFGVSRTLVRE
                     ALMQLQARGFVEVRTRKGWYVVEPSIAEARDAFAARRAVESGMLRESDVLRDPGKPLQ
                     SVIKRLRQHVREEEAAIASADV"
     gene            complement(411..1718)
                     /locus_tag="VFA35_03130"
     CDS             complement(411..1718)
                     /locus_tag="VFA35_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HZT55192.1"
                     /translation="MPRFLRSLFGQVVLALVVGVLLGLLVPEFALKLKPLGDSFIKLI
                     KMIIPVLVFCVVVHGIAGAGDLKRVGRVGVKALVYFEVVTSIALGLGLVLAFVFQPGA
                     GMNVDPHTLDAKAMSAYAENASKLTGGGTVDFLMKLIPTTVGDAFAKGDVLQVLLFAI
                     LFGCALALIGERGKAVAHFIDDLSHVIFKIMGILIKLAPLGVLGAIAFTVAKYGIGSL
                     RQLGMLVALFYAAVAIFVFVILGAILRVCGFSIVKLLKYLREELAVVFATTSSDSVLP
                     QIMAKLKYMGIRDSTVGLVIPTGYSFNLDAFSIYITLAAVFIAQATNTPISMSDLLTI
                     LAISLVTSKGAHGVPGSAIVVLAATLQAIPAIPAIGLVLVLSVDWFMGIARALGNLIG
                     NCVATVAVAAWEGDIDRERAHAVLDGRPVVDDAEARVLAAATH"
     gene            complement(1842..2054)
                     /locus_tag="VFA35_03135"
     CDS             complement(1842..2054)
                     /locus_tag="VFA35_03135"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HZT55193.1"
                     /translation="MPTIRVEMFEGRTPDQKRALVAALTDACVRTLGTSPDSVDILLF
                     DIAKQDWATGGTLWSDKGAAPKPGGS"
     gene            complement(2083..2823)
                     /locus_tag="VFA35_03140"
     CDS             complement(2083..2823)
                     /locus_tag="VFA35_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZT55194.1"
                     /translation="MKPARTERDVRIDLAAAYRLAAQRGWDDTVYTHLSASVPGEPGV
                     YLINPFGARFDEITAASLVKVDTRGEVVDGSGRRVNPSGFAIHGAVHAARPELECVVH
                     LHTTAGVALSMLPGGLQPTSQWAMRLHGRLGRHAYEGLAFGADERARLVANLGALDGL
                     ILENHGPLTVGRTVAEAWMLMYILERAAQAQLAALAATGGRVSVVSDALAALTYRQWV
                     GDGSERDGDLEWPALLRGLDASSPGYRD"
     gene            complement(2839..4197)
                     /locus_tag="VFA35_03145"
     CDS             complement(2839..4197)
                     /locus_tag="VFA35_03145"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HZT55195.1"
                     /translation="MSTLLIKNAQLLVTMDAQRREIAGGAVFVRDNVIEAVGTTADLP
                     DTADEVIDATDQIVIPGLVNTHHHMVQTLTRAFAQDDELFGWLRSLYPVWANLTPEMV
                     KVSTQAAMAELLLSGCTTSSDHLYLYPNGIRLEDSIEAAAEIGMRFHAARGAMSVGES
                     QGGLPPDRLVEDEAAILEDTQRLIERWHDPARFAMQRIVVAPCSPFSVSRALMRDAAL
                     LARQYGVSLHTHLAENDFDVAYTREKFGCTPAEYVEQLGWTGPDVWCAHCVKLDAHGV
                     ALFARTGTGVAHCPCSNMRLASGIAPIRAMRDAGVSVGLGVDGAASNDAGHLLAEARM
                     AMLLQRVGSEKGPAAMGAREVLEIATLGGAKVLNRDDIGALAVGMAADIVSVPLDGIA
                     TAGALHDPLAALVLCQIPRVKHTIVNGRVIVREGELTTLALPRLVARHNALARTLANA
                     AR"
     gene            complement(4264..5754)
                     /locus_tag="VFA35_03150"
     CDS             complement(4264..5754)
                     /locus_tag="VFA35_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HZT55196.1"
                     /translation="MSATPSAVHPVDERLPTGRLAALGLQHVLVMYAGAVAVPLIVGR
                     ALKLSPEQVTMLISADLFCCGLVTLIQALGATPWFGIKLPVMMGVTFASVAPMVAMAN
                     SAPEGQGAGLIFGSVIGAGVVSIAIAPLVSRMLRFFPPVVTGTIIATIGISLMRVGIN
                     WIFGNPVGPTAPTIVAPEHAKWLAEVASAAASAGVPAPPKGLALVGSVPNPKYAPLGP
                     IAISGVSLAAILLIAKFAKGFIANISVLLGIVAGGVLAAAMGMMSFEKVGKAAWFDLV
                     LPFEIALPVFEPVLILTMSLVMIVVMIESTGMFLALGDMTGTSVDQKMLTRGLRTDGL
                     GTLIGGIFNTFPYTSFSQNVGLVGVTGVKSRYVCVAGGVILMLLSLLPKMAALVESLP
                     TFVLGGAGLVMFGMVAATGIRILGTVDYKNNRNNLFIVAISIGVGMVPLIAPNFKQWM
                     PHGLHPLIESGILLASLCAFALNLFFNGASGNAAEAVEAAKHAEAH"
     gene            complement(5770..>6824)
                     /locus_tag="VFA35_03155"
     CDS             complement(5770..>6824)
                     /locus_tag="VFA35_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HZT55197.1"
                     /translation="KGVGGEQWGVHGGGFNHHQKYPVSPGYVPDQLHWSMWESYSTWL
                     TGFALFTVLYLFNASTFLIDKTLFDWTPTAAICTALAFFVAFWVLYDTICRTLGRGKH
                     GDMIVGGCVFVLVVVASWLSCHLFAGRAAFLLVGAMIATTMSGNVFFWIIPGQRTVVA
                     AIKAGQPVDPVHGQRGKQRSVHNTFFTLPVLFAMMSNHYGFTYGTKHNWLVLVAIMVA
                     GALIRFSFALRHKALAFGKPVPWHYAIVGTLLLLGTVWAVRPEPQPVAAAAAPATFAQ
                     VQGIVSERCVMCHGAAVQSKNVRLDSADEIARHAQQIYQQAVVLKTMPLNNATQITEA
                     ERALIGRWFTAGAATK"
BASE COUNT         1118 a         2347 c         2357 g         1002 t
ORIGIN      
        1 gacgtcggcg ctggcgatcg cggcctcttc ctcgcggaca tgctggcgca gccgcttgat
       61 caccgactgg agaggcttgc cggggtcgcg cagcacgtcg gattcgcgca gcatgccgct
      121 ctcgaccgcg cgccgcgccg cgaacgcgtc gcgcgcctcg gcgatcgacg gctcgaccac
      181 gtaccagccc ttgcgggtgc gcacctcgac gaagccgcgc gcctgcaact gcatcagcgc
      241 ctcgcgcacc agcgtgcggc tgacgccgaa catgtcggcc agttcctgct cgccgaggcg
      301 ctcgccgggc ttgagctttt gcgcgaggat cgactcgacg acgcgctggg cgatttcgat
      361 ctgggtggcc atgggggagc gaggttagca gcgcgccggc gctcgatcgc tcaatgcgtc
      421 gcggcggcca gtacccgggc ctcggcgtcg tcgacgaccg gccggccgtc gagcaccgcg
      481 tgggcgcgct cgcggtcgat gtcgccctcc caggctgcca ccgcgacggt ggccacgcag
      541 ttgccgatca ggttgcccaa ggcacgggca atgcccatga accagtcgac cgacagcacc
      601 agcaccagcc cgatcgcggg gatcgcggga atcgcctgca gcgtcgccgc gagcaccacg
      661 atcgccgagc ccggcacgcc gtgcgcgccc ttcgaggtga cgagcgagat cgccagtatc
      721 gtcagcaggt cgctcatcga gatcggcgtg ttggtggcct gcgcgatgaa caccgccgcc
      781 agcgtgatgt agatcgagaa cgcgtcgagg ttgaacgagt agccggtcgg gatcaccagg
      841 ccgacggtcg agtcgcggat gcccatgtac ttgagcttgg ccatgatctg cggcagcacg
      901 ctgtcggacg aggtggtcgc gaacacgacc gcgagctcct cgcgcaggta cttcagcagc
      961 ttgacgatcg agaagccgca aacccgcagg atcgccccca ggatcacgaa cacgaagatc
     1021 gcgaccgcgg cgtagaacag cgccaccagc atgccgagtt gcctgagcga accgatgccg
     1081 tacttggcga ccgtgaacgc gatcgcgccg agcacgccca gcggcgcgag cttgatcagg
     1141 atgcccatga tcttgaagat cacgtgcgac aggtcgtcga tgaagtgcgc gaccgccttg
     1201 ccgcgctcgc cgatcagcgc cagcgcgcag ccgaacagga tcgcgaacaa cagcacctgc
     1261 agcacgtcgc ccttcgcgaa cgcatcgccc acggtcgtgg ggatcagctt catcaggaag
     1321 tcgaccgtgc cgccgccggt gagcttgctc gcgttctcgg cataggcgct catcgccttg
     1381 gcatcgagcg tgtgcgggtc gacgttcatg ccggcgccgg gctggaacac gaaggccagc
     1441 accaggccga ggccgagcgc gatcgaggtc acgacctcga agtacaccag cgccttgacg
     1501 ccgacccggc ccacgcgctt caggtcgccc gcgcccgcga tgccgtgcac gacgacgcag
     1561 aacaccagca ccgggatgat catcttgatc agcttgatga agctgtcgcc gagcggcttg
     1621 agcttgagcg cgaactcggg caccaacagg ccgagcagca cgccgaccac cagcgcgagc
     1681 acgacctggc cgaacaggga acggaggaaa cggggcatgg gatgttctcc agcgggtggg
     1741 gcagatgggc acgttgacaa cgcttgcata caagaaatgt agtcaacttg cccaggatcg
     1801 cccatgcggg tttccccgcc ctcgtcgggc cgcgctggcg ctcaggagcc gccgggtttg
     1861 ggcgcggcgc ccttgtcgct ccacaaggtg ccgccggtcg cccagtcctg cttcgcgatg
     1921 tcgaacaaca aaatgtcgac gctgtcgggc gaggtgccga gcgtgcgcac gcaggcatcg
     1981 gtcagcgccg ccaccagcgc gcgcttctgg tccggcgtgc ggccttcgaa catctcgacg
     2041 cggatcgtgg gcatggggtc gtctccttcg gtcgtgggtt catcaatcgc gatacccggg
     2101 gctgctcgca tcgagcccgc gcagcagtgc cggccattcg aggtcgccgt cgcgctcgct
     2161 gccgtcgccg acccactggc ggtaggtgag cgccgcgagc gcgtcgctga ccacgctgac
     2221 gcgcccgccg gtcgcggcca gcgccgcgag ctgcgcctgg gcggcgcgtt cgaggatgta
     2281 catcagcatc caggcctcgg cgaccgtgcg gccgaccgtc agcgggccgt gattctcgag
     2341 gatcaggccg tcgagcgcgc ccaggttcgc gaccaggcgg gcccgctcgt cggcgccgaa
     2401 cgcgaggccc tcgtaggcgt ggcggccgag ccggccgtgc agccgcatcg cccactgcga
     2461 ggtcggctgc aggccgcccg gcagcatgct cagcgcgacg ccggcggtgg tgtgcaggtg
     2521 cacgacgcat tcgagctcgg gccgcgccgc gtgcaccgcg ccgtggatcg cgaagccgct
     2581 cgggttgacg cgccggccgc tgccgtcgac gacctcgccg cgggtgtcga ccttcaccag
     2641 gctggccgcg gtgatctcgt cgaagcgcgc gccgaacggg ttgatcaggt agacgccggg
     2701 ttcgcccggc acgctggccg acaggtgggt gtagaccgtg tcgtcccaac cgcgctgcgc
     2761 ggcaaggcgg taggcggcgg cgaggtcgat gcggacgtcg cgctcggtgc gggcgggctt
     2821 catcgcccgc agcgtacctc agcgtgcggc gttcgccagg gtgcgggcga gcgcgttgtg
     2881 gcgcgcgacc agccgcggca gcgcgagcgt ggtcagctcg ccctcgcgca cgatcacccg
     2941 gccgttgacg atcgtgtgtt tcacgcgcgg gatctggcaa agcaccagcg cggcgagcgg
     3001 gtcgtgcagc gcaccggcgg tggcgatgcc gtcgagcggc acgctgacga tgtcggccgc
     3061 catgccgacc gcgagcgcgc cgatgtcgtc gcggttcaac accttggcgc cgccgagcgt
     3121 cgcgatctcg aggacctcgc gcgcacccat cgccgccggg cctttctcgg aaccgacccg
     3181 ctgcagcagc atcgccatac gcgcttcagc gagcaggtgg ccggcgtcgt tcgaggccgc
     3241 gccgtcgacg ccgaggccga cgctcacgcc ggcatcgcgc atcgcgcgga tcggtgcgat
     3301 gcccgaggcg agccgcatgt tcgagcacgg gcaatgcgcg acgccggtgc cggtgcgcgc
     3361 gaacagcgcg acgccgtgcg cgtcgagctt gacgcaatgc gcgcaccaca cgtccgggcc
     3421 ggtccagccg agttgttcga cgtactcggc cggcgtgcag ccgaacttct cgcgcgtgta
     3481 ggcgacatca aagtcgttct cggccaggtg cgtgtgcagc gagacgccgt actgccgtgc
     3541 gagcagcgcc gcgtcgcgca tcagggcgcg actgaccgag aacggcgagc acggcgcgac
     3601 gacgatgcgc tgcatcgcga agcgcgccgg gtcgtgccag cgctcgatca gccgctgcgt
     3661 gtcttcgagg atcgccgctt cgtcttcgac cagccggtcc ggcggcaggc cgccctgcga
     3721 ttcgccgacg ctcatcgcgc cgcgcgcggc gtgaaagcgc atgccgatct cggcggcagc
     3781 ctcgatgctg tcctccagcc tgatgccgtt cggatagagg tacaggtggt cgctgctggt
     3841 ggtgcagccc gagagcagca gctcggccat cgccgcctgg gtgctgacct tcaccatctc
     3901 gggcgtcagg ttcgcccaca ccgggtagag gctgcgcagc cagccgaaca gttcgtcgtc
     3961 ctgcgcaaac gcgcgcgtca gcgtctgcac catgtggtgg tgcgtgttga ccaggccggg
     4021 gatcacgatc tgatcggtgg cgtcgatcac ctcgtcggcg gtgtccggca aatcggcggt
     4081 cgtgccgacg gcctcgatca cgttgtcgcg cacgaacacc gcgccgcccg cgatctcgcg
     4141 gcgctgcgcg tccatcgtga cgaggagttg cgcgttcttg atcagcaggg tggacatcgg
     4201 ggctcttgaa agatcaaggt caagagagtt gtcgcaaaga gaactgtgga gccgctcggt
     4261 ccctcagtgc gcctcggcgt gtttggccgc ttccaccgct tcggcggcgt tgccgctcgc
     4321 gccattgaag aacaggttca acgcgaacgc gcacagcgag gccagcagga tgcccgactc
     4381 gatcagcggg tgcaggccat gcggcatcca ctgcttgaag ttcggcgcga tcagcgggac
     4441 catgccgacc ccgatcgaga tcgcgacgat gaacaggttg ttgcggttgt tcttgtagtc
     4501 caccgtgccg aggatgcgga tgccggtggc cgcgaccatg ccgaacatca cgaggccggc
     4561 gccgccgagc acgaaggtcg gcaacgattc gaccagcgcg gccatcttgg gcagcaggct
     4621 gagcagcatc aggatcacgc cgccggccac gcagacgtag cggctcttca cgccggtgac
     4681 gccgaccagg ccgacgttct gcgagaagct ggtgtacgga aaggtgttga agatgccgcc
     4741 gatcagcgtg cccaggccgt cggtgcgcag gccgcgggtc agcatcttct ggtcgaccga
     4801 cgtgccggtc atgtcgccga gcgcgaggaa catgccggtc gactcgatca tcacgacgat
     4861 catcaccagc gacatcgtca ggatcagcac cggctcgaac accggcagcg cgatctcgaa
     4921 cggcagcacc agatcgaacc acgcggcctt gccgaccttc tcgaagctca tcatccccat
     4981 cgcggcggcg agcacgccgc cggcgacgat gccgagcagc accgagatgt tcgcgatgaa
     5041 gcccttcgca aacttcgcga tcagcaggat cgccgcgagc gagacgcccg agatcgcgat
     5101 cgggccgagc ggcgcgtatt tcgggttcgg cacgctgccg accagcgcga ggcccttcgg
     5161 cggcgccggc acgcctgccg aggcggctgc actcgcaacc tcagccagcc acttggcatg
     5221 ctcgggcgcg acgatcgtcg gcgcggtcgg gcccacggga ttgccgaaga tccagttgat
     5281 gcccacgcgc atcaggctga tgccgatggt cgcgatgatc gtgccggtga cgaccggcgg
     5341 aaagaagcgc agcatccggc tgaccagcgg cgcgatcgcg atcgagacca cgccggcgcc
     5401 gatcaccgag ccgaagatca gcccggcccc ctggccctcg ggcgcgctgt tcgccatcgc
     5461 caccatcggc gcgacggagg cgaaggtcac gcccatcatc accggcagct tgatgccgaa
     5521 ccatggggtc gcgccgagcg cctggatcag cgtcaccagc ccgcagcaga acaggtcggc
     5581 cgagatcagc atcgtcacct gctcgggact gagcttcagc gcccggccga cgatcagcgg
     5641 caccgcgacc gcgccggcgt acatcacgag cacgtgctgc aagccgagcg cggcgagccg
     5701 cccggtgggc aggcgctcat cgacggggtg cacggcggaa ggtgtggcgc tcatgtcgtt
     5761 cctctcgggc tacttcgtcg cggcgccggc cgtgaaccag cgcccgatca gcgcgcgctc
     5821 ggcctcggtg atctgggtcg cgttgttcag cggcatggtc ttcagcacca ccgcctgctg
     5881 gtagatctgc tgcgcgtgcc gggcgatctc gtcggccgag tcgaggcgca cgttcttgct
     5941 ctgcaccgcg gcaccgtggc acatcacgca gcgctcggag acgatgccct gcacctgcgc
     6001 gaacgtggcc ggcgccgcgg cggccgccac cggctgcggc tcgggccgca cggcccagac
     6061 cgtgccgagc agcagcaaag tgccgacgat cgcgtagtgc cagggcaccg gcttgccgaa
     6121 ggccagcgcc ttgtggcgca gcgcgaacga gaagcggatc agcgcgcccg ccaccatgat
     6181 cgcgaccagt accagccagt tgtgcttcgt gccgtaggtg aagccgtagt ggttgctcat
     6241 catcgcgaac aacaccggca acgtgaagaa ggtgttgtgc acgctgcgct gcttgccgcg
     6301 ctggccgtgc accgggtcga ccggttgccc cgccttgatc gcggcgacga ccgtgcgctg
     6361 gcccggaatg atccagaaga acacgttgcc gctcatcgtc gtcgcgatca tcgcgccgac
     6421 cagcaagaag gcggcgcgcc cggcgaacag gtggcacgag agccacgacg cgaccacgac
     6481 cagcacgaac acgcagccgc cgacgatcat gtcgccgtgc ttgccgcggc cgagcgtgcg
     6541 gcagatcgtg tcgtagagca cccagaacgc gacgaagaac gccagcgcgg tgcagatcgc
     6601 cgcggtcggc gtccagtcga acagcgtctt gtcgatcagg aaggtgctgg cgttgaacag
     6661 gtagagcacc gtgaacagcg cgaaaccggt caaccaggtc gagtagctct cccacatcga
     6721 ccagtgcagc tgatcgggca cgtagccggg cgagaccggg tacttctggt ggtggttgaa
     6781 gccgccgccg tgcacgcccc attgctcgcc gccgacgccc ttgg
//
LOCUS       DASRDG010000192         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_389669, whole genome shotgun sequence.
ACCESSION   DASRDG010000192 DASRDG010000000
VERSION     DASRDG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389669"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1289)
                     /locus_tag="VFA35_03160"
     CDS             complement(48..1289)
                     /locus_tag="VFA35_03160"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT55198.1"
                     /translation="MTRPHPRQPAFAALQHRGYRGFVLGNAVAMMADNTEHVISYWVL
                     YQKFHSPALGAFAVISHWVPFLLFSGMAGALADRFDPRRIIQLGMLLFMAVSVGWGLL
                     FLGGHPQLWQCWLLLALHGIAGVLWTPTSQVLVYDIVGPEHLQSAVRLNASGRYLGTL
                     LGPAAGNLLMIALGPEYGILLNALVYLPLLLWLRKTRFEHHAGAVPREARPAAGWGEI
                     VATFRSLTGNRQLMAMTMLAGATSLFVGNAYQAQMPGFAEDLGLRSVGTSYAALLGAD
                     AAGAIAAALLLEGGGLLPATPGGALWLGALWCLAIGGFALSGGYAVTLPLLFAAGFFE
                     LTFGAMAQTIVQLHAPPARRGRIIGLYVTASLGLRAFSGVSVGLGATLIGVHDALALS
                     AAALLACIGAIAWWYRRAAAR"
     gene            complement(1286..2272)
                     /locus_tag="VFA35_03165"
     CDS             complement(1286..2272)
                     /locus_tag="VFA35_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZT55199.1"
                     /translation="MASRRRSARPTRWSTACPSAERSIAMTSDTGPSPATPERTIPGL
                     TNARRERVRALFRFVSALSPGLAAWLAMQMFLTPLARRIAPSEARFLATAKPLHLQLP
                     SGRLHAYEWPAESPDAPTVLLVHGWISHAARMAELIRALRQRGLRVVAFDAPAHGRSG
                     GRQADLQSFRAAIDAVLAACGAVHGVLAHSFGALTTSTWLAEDRPGGVRAAVLVGMMR
                     DAGYVFESFALTMALQPDVLARFRELFRARYGIYPEELSTTALVRRLSLPVLVVHGEL
                     DELVPVEHASEIASQLHDGQLLIAPLLGHGAPLRDPATVTRICDFLVARLAT"
     gene            complement(2160..>2422)
                     /locus_tag="VFA35_03170"
     CDS             complement(2160..>2422)
                     /locus_tag="VFA35_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HZT55200.1"
                     /translation="PGTVQPGREITGLERAAQLPGKVFHAGTELSAGRIVTNGGRVLC
                     AVGLGDGVAQAQRAAYALVDCLSFRGAQYRHDIGHRAIARDS"
BASE COUNT          410 a          836 c          888 g          288 t
ORIGIN      
        1 cggcggagag ctgttcgtgc gcgcaagctt ctaggcgcgc gaactggtca acgcgcggcc
       61 gcgcgccggt accaccatgc gatcgcgccg atgcaggcga gcagcgccgc ggcgctcagc
      121 gccagcgcgt cgtgcacgcc gatcagcgtt gctccgagcc cgaccgatac gccgctgaag
      181 gcccgcaggc ccaggctggc cgtgacgtac aggccgatga tccgtccacg tcgcgcgggc
      241 ggggcgtgca gctgcacgat agtctgcgcc atcgcgccga aggtgagttc gaagaagccg
      301 gcggcaaaca acaacggcaa cgtcacggcg tagccgcccg agagcgcgaa gccgccgatg
      361 gcgaggcacc agagcgcgcc cagccacaag gcgccgcccg gcgtcgccgg cagcaatccg
      421 ccaccctcga gcagcagcgc tgcggcgatc gcgccggcgg cgtccgcgcc gagcagcgcg
      481 gcgtacgagg tcccgacgct gcgcaggccc aggtcttcgg cgaagcccgg catctgcgcc
      541 tggtaggcgt tgcccacgaa cagcgaggtg gcgccggcca gcatcgtcat ggccatgagc
      601 tggcgattgc cggtgaggct gcggaacgtg gcgacgatct cgccccagcc ggccgcgggc
      661 cgggcctcgc gcggcacggc gccggcgtga tgctcgaatc gcgtcttgcg gagccagagc
      721 agcagcggca ggtagaccag ggcgttgagg aggatgccat attcggggcc gagcgcgatc
      781 atgagcaggt tgccggcggc cgggcccagc agcgtcccga ggtagcggcc gctcgcgttc
      841 aggcgaacgg cgctctgcag gtgttcgggc ccgacgatgt cgtagaccag cacctgcgag
      901 gtaggcgtcc acagcacgcc ggcgatgccg tgcagcgcga gcagcagcca gcactgccaa
      961 agttgcggat ggccgccgag gaacagcagt ccccaaccga ccgagacggc catgaagagc
     1021 aacatgccga gctggatgat gcggcgcgga tcgaagcgat cggccagcgc gccggccatg
     1081 cccgagaaca gcaggaacgg gacccagtgc gagatcaccg cgaacgcgcc cagtgccggc
     1141 gagtggaact tctggtagag cacccagtag ctgatcacgt gctcggtgtt gtcggccatc
     1201 atcgcgacgg cgttgccgag cacgaagccg cggtagccgc gatgctggag tgcggcgaag
     1261 gcgggctggc gcgggtgcgg acgcgtcacg tcgcgaggcg cgcgaccagg aagtcacaga
     1321 tccgcgtcac cgtcgccgga tcgcgcaagg gtgcgccgtg cccgaggagc ggggcgatca
     1381 gcagctgacc gtcgtgcaac tgcgatgcga tctcgctggc gtgctcgacc ggaacgagct
     1441 cgtcgagctc gccgtgcacg accagcacgg gcagcgaaag gcgccgcacg agcgcggtcg
     1501 tcgacagctc ctccgggtat atgccgtagc gcgcgcggaa cagctcgcgg aaccgggcca
     1561 gcacatccgg ctgcagcgcc atcgtcagcg cgaaggattc gaacacgtag ccggcgtcgc
     1621 gcatcatccc gaccagtacc gccgcccgca cgccaccagg acgatcctcg gccaaccagg
     1681 tggaggtcgt gagcgcaccg aaggagtggg cgagcacgcc gtgcaccgca ccgcaggcgg
     1741 ccagcacggc gtcgatggcg gcgcggaacg actgcaggtc ggcctgccgt ccgcccgagc
     1801 ggccgtgtgc cggcgcgtcg aaggcgacca ctcgcagccc gcgctggcgc agcgcgcgaa
     1861 tcagctcggc catgcgggcg gcgtgtgaaa tccagccatg cacgagcagc acggttggcg
     1921 catccggaga ctcggccggc cattcgtagg cgtgcaatcg cccgctcggc aactgcaggt
     1981 gcagcggctt cgcggtggcg aggaagcgcg cctcggatgg cgcgatgcgg cgcgccagcg
     2041 gggtcaggaa catctgcatc gccagccatg cggcgaggcc gggactcagc gcgctgacga
     2101 atcggaacag cgcccgcacg cgctcgcgcc gcgcgttggt gagcccgggt atggtgcgtt
     2161 caggagtcgc gggcgatggc ccggtgtccg atgtcatggc gatactgcgc tccgcggaag
     2221 gacaggcagt cgaccagcgc gtaggccgcg cgctgcgcct gcgcgacgcc atcacccagt
     2281 ccgacggcgc acagcacgcg cccgccattc gtgacgatgc ggcccgcgct caactcggtg
     2341 ccggcgtgga acaccttgcc cgggagctgc gcagcgcgct ccaggcccgt gatctcgcgc
     2401 cctggctgca ccgtgcccgg at
//
LOCUS       DASRDG010000193         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1290135, whole genome shotgun sequence.
ACCESSION   DASRDG010000193 DASRDG010000000
VERSION     DASRDG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290135"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VFA35_03175"
     CDS             complement(<1..999)
                     /locus_tag="VFA35_03175"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZT55201.1"
                     /translation="MSQALHPMLNTAIKAARSAGAIINRASLDLDTLKVDTKSPNDFV
                     TEVDRRAEAVIIETLLGAYPGHGILAEESGREHGAKDSEYVWIIDPLDGTTNFIHGFP
                     VYSVSIALAFRGQVQQGVVYDPSRNDLFYASKGRGAFLNDRRLRVSKRTRLADCLVST
                     GFPYRKGDNFKRYLQMLEVTMPACAGLRRPGSAALDLCYVAAGYTDGFFENGLSPWDI
                     AAGSLMITEAGGLIGNFTGEADYLYQRECLAASPRIYGQLVALLSPFTHVIAADAALA
                     PGETSVGITPEAALAAGVTIQRDPGAPAPARKAATRISKESMAAEKQAAADAERKVP"
     gene            1111..1854
                     /locus_tag="VFA35_03180"
     CDS             1111..1854
                     /locus_tag="VFA35_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZT55202.1"
                     /translation="MPDPRAPDPPDATRFILIGTSHPGNVGAAARAMKVMGFSDLVLV
                     APRCADVLAQADAHAMASGATDVLARARIVRTLADALDGITWACATAMTPRDFGPPTR
                     APRELFAELAAGTHRVALVFGSERFGMSNDDVYRCHVCLSIPTLPEYGSLNLAQAVQL
                     LAYDWRQALGGFAVRPRTVDARRADDAAVQGVLDHWRQTLIDIDFLDPAVPRKLMPRL
                     QQLLNRAQLTEEEVHILRGVARAVDKARR"
     gene            1942..>2591
                     /gene="cysE"
                     /locus_tag="VFA35_03185"
     CDS             1942..>2591
                     /gene="cysE"
                     /locus_tag="VFA35_03185"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZT55203.1"
                     /translation="MFQRLREDIACILERDPAARSAWEVLTCYPGLHALILHRRARWF
                     HVNGMHWLARFTSHIGRFLTGIEIHPGAVIGRRVFIDHGMGVVVGETAEIGDDCTIYQ
                     GVTLGGTSLAKGAKRHPTLGRGVIVGANSQVLGGFTVGDGARVGSNAVVLKEVPPGAT
                     AVGNPARVLHKELDAVREAAAAKMGFSAYGITQGDDPLAHAMKGLIDNASGHEHQIA"
BASE COUNT          366 a          879 c          958 g          388 t
ORIGIN      
        1 cggcaccttg cgctcggcgt cggcggcggc ctgcttctcg gcggccatgc tttccttgct
       61 gatgcgggtg gcggccttcc tggccggtgc cggcgcgccg gggtcgcgct ggatcgtgac
      121 gccggcggcg agcgccgcct cgggcgtgat gccgacgctg gtctcgcccg gtgccagcgc
      181 cgcgtcggcc gcgatcacgt gcgtgaacgg gctcagcagc gcgaccagct ggccgtagat
      241 gcgcgggctc gcggccaggc attcgcgctg gtagaggtag tcggcctcgc cggtgaagtt
      301 gccgatcagc ccgccggctt cggtgatcat cagcgagccg gcggcgatgt cccaagggga
      361 caggccgttc tcgaagaagc cgtcggtgta gccggcggcg acgtagcaca ggtcgagcgc
      421 ggccgagccc gggcggcgca ggccggcgca ggccggcatc gtcacctcga gcatctgcag
      481 gtagcgcttg aagttgtcgc ccttgcggta cgggaagccg gtggagacca ggcagtcggc
      541 cagccgcgtg cgcttcgaga cgcgcagccg gcggtcgttc aggaacgcac cgcggccctt
      601 cgaagcgtag aacaggtcgt tgcgcgacgg gtcgtagacg acgccctgct gcacctggcc
      661 gcgaaacgca agcgcgatcg agaccgagta gaccgggaag ccgtggatga agttggtggt
      721 gccgtcgagc gggtcgatga tccagacgta ctcgctgtcc ttggccccgt gttcgcgccc
      781 cgattcttcg gcgaggatgc cgtggcccgg gtaggcgccg agcagcgtct cgatgatgac
      841 cgcctcggcc cggcggtcga cctcggtgac gaagtcgttc ggcgacttgg tgtcgacctt
      901 gagggtgtcg aggtcgagcg aggcccggtt gatgatcgcc ccggcgctgc gggccgcctt
      961 gatggcggtg ttgagcatgg ggtgcagggc ttgggacatg ggcgacctat cgagggcagg
     1021 agggcgtgaa acggggggct ggcggggaca aagagcggca cggcgcgcgg agcgctgcgg
     1081 ataatccaca ttttacctgc gagctcccac gtgcctgacc cacgcgcgcc agacccaccc
     1141 gacgcgaccc gcttcatcct gatcggcacg agccaccccg gcaatgtcgg cgccgcggcg
     1201 cgcgcgatga aggtgatggg cttctctgac ctcgtgctgg tcgcgccgcg ctgcgccgat
     1261 gtgctggccc aggctgacgc gcacgcgatg gcgagcggcg cgaccgacgt gctggcgcgc
     1321 gcccgcatcg tgcgcacgct cgccgacgcg ctcgacggca tcacctgggc ctgcgcgacc
     1381 gcgatgacgc cgcgcgactt cgggccgccg acgcgtgcgc cgcgcgagct gttcgccgag
     1441 ctcgcagccg gcacgcaccg cgtcgcgctg gtgttcggct cggagcgttt cggcatgagc
     1501 aacgacgacg tgtaccgctg ccatgtgtgc ctgagcatcc cgacgctgcc cgagtacggc
     1561 tcgctgaacc tggcccaggc ggtgcagttg ctggcctacg actggcgcca ggcgctcggc
     1621 ggctttgcgg tccggccgcg cactgtcgat gcgcgccgcg ccgacgacgc ggccgtgcaa
     1681 ggcgtgctcg accactggcg ccagacgctg atcgacatcg attttctcga ccccgccgtg
     1741 ccgcgcaagc tgatgccgcg gctgcaacag ctgctgaacc gggcgcagct gactgaagag
     1801 gaggtgcaca tcctgcgcgg cgtggcgcgc gcggtcgaca aggcgcggcg ctgacgccac
     1861 gccgggcggg tcttcgcgcg cccgcgctgc gccggccggg cgcagcggat aaactgcgcc
     1921 accccattca ccggactcgt catgttccag cggctgcgag aagacatcgc ctgcatcctc
     1981 gagcgcgacc ctgcagcgcg cagcgcgtgg gaggtgctga cgtgttaccc gggcctgcat
     2041 gcgctgatcc tgcaccgccg tgcgcgctgg tttcacgtca acggcatgca ctggctggcg
     2101 cgcttcacgt cgcacatcgg ccgcttcctg acgggcatcg agatccaccc cggcgcggtg
     2161 atcggccgac gcgtcttcat cgaccacggc atgggcgtgg tcgtcggcga gaccgccgag
     2221 atcggcgacg actgcacgat ctaccagggc gtgaccctgg gcggcacctc gctcgcgaag
     2281 ggcgcgaagc gccaccccac gctggggcgc ggcgtgatcg tcggcgccaa ttcgcaggtg
     2341 ctgggcggct tcacggtcgg cgatggcgca cgggtcggct cgaacgcggt cgtgctgaag
     2401 gaagtgccgc ccggcgcgac cgcggtcggc aacccggcgc gcgtgctgca caaggagctt
     2461 gatgcggtgc gcgaggccgc cgccgcgaag atgggtttct cggcctacgg catcacgcag
     2521 ggcgacgatc cgctggcgca cgcgatgaaa ggcctgatcg acaacgcctc ggggcacgaa
     2581 caccagatcg c
//
LOCUS       DASRDG010000194         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1060945, whole genome shotgun sequence.
ACCESSION   DASRDG010000194 DASRDG010000000
VERSION     DASRDG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060945"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFA35_03190"
     CDS             <1..1109
                     /locus_tag="VFA35_03190"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HZT55204.1"
                     /translation="VMIIDNLVQSSDAHAVAQRALAALHQPLRIGDKDVHSSASIGIA
                     LYPQDGTSVEALMAHADAAMYCVKQQGRNDVQFFTPGMDAATLDRGKLESDLHHALAR
                     KQFVLHYQPKVEADSGQLRGAEALIRWQHPERGLMTPASFIPLAEECGLIEAIGNWVI
                     REACRQARAWQDEGLTPTRVAVNLSAFQFRNGTLLGTIRDALRDNRLDPAYLEVEITE
                     SALMSDPEESAGILRKLSEMGVLVSVDDFGTGYSSMSYLQRFPIDKLKIDRTFISDVT
                     SSNDDASIVSAIVSLGHTLKLKVIAEGVETPEQLEFLQRLGCDQYQGYHYSKPMPAHE
                     FAELIRRGGHVQDLSAADDDPGRTQSRLSLKRPA"
     gene            complement(1185..1301)
                     /locus_tag="VFA35_03195"
     CDS             complement(1185..1301)
                     /locus_tag="VFA35_03195"
                     /inference="COORDINATES: protein motif:HMM:NF028380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HZT55205.1"
                     /translation="MGKGDRKTRRGKIYAGTFGKSRPKKPKRKTAAKKSVRK"
     gene            complement(1407..>2095)
                     /locus_tag="VFA35_03200"
     CDS             complement(1407..>2095)
                     /locus_tag="VFA35_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZT55206.1"
                     /translation="IHAIRRNLNMVYIIENNGVYGLTKGQFSASADIGSTAKKGEVNN
                     QSPIDPVQLALTLGASFVARSFSGDKAQLVPLIQAAMHHSGFALIDVLSPCVTFNDHE
                     GSTRSYQYSRDHYEEVVHADLVAPAAEISVQYAPGESVPVTMHDGSRIVLRKVDPDYD
                     PTDRGAALARIRERLRAQEYLTGLLHISAGQPEFHELNVTPDEALNEIPYERLNPGAA
                     ALAKTLARFR"
BASE COUNT          341 a          706 c          711 g          337 t
ORIGIN      
        1 tcgtcatgat catcgacaac ctggtgcagt ccagcgatgc gcacgccgtg gcgcagcgcg
       61 cactcgcggc cctgcaccag ccgctgcgca tcggcgacaa ggacgtccac agctcggcca
      121 gcatcggcat cgcgctctac ccgcaggacg gcaccagcgt cgaggcgctc atggcgcacg
      181 ccgatgccgc gatgtactgc gtcaagcagc agggccggaa cgacgtgcag ttcttcacgc
      241 ccggcatgga cgccgccacg ctcgatcgcg gcaagctcga aagcgacctg caccacgcgc
      301 tggcgcgcaa gcagttcgtg ctgcactacc agcccaaggt cgaggccgac agcggccagc
      361 tgcgcggcgc cgaggcgctg atccgctggc agcacccgga acgggggttg atgacgccgg
      421 cgagcttcat cccgctggcg gaggaatgcg gcttgatcga ggccatcggc aactgggtga
      481 tccgcgaggc ctgccgccag gcgcgcgcct ggcaggatga aggcctgacg ccgacacgcg
      541 tggccgtcaa cctgtcggcg ttccagttcc gcaacggcac gctgctcgga acgatccgcg
      601 acgcgctccg ggacaatcgg ctcgatccgg cctacctcga agtcgagatc accgagagcg
      661 ccctcatgag cgatcccgag gaatcggccg gcatcctgcg caagctcagc gagatgggcg
      721 tgctggtgtc ggtcgacgac ttcggcaccg gttactcgag catgagttac ctgcagcgct
      781 tcccgatcga caagctcaag atcgaccgca ccttcatcag cgacgtgacc tcgagcaacg
      841 acgacgcctc gatcgtcagc gcgatcgtgt cactcggcca cacgctcaag ctcaaggtca
      901 tcgccgaggg cgtcgagacc cccgagcagc tcgagttcct gcagaggctc ggttgcgacc
      961 agtaccaggg ctaccactac agcaagccga tgccggcgca cgaattcgcc gagctcattc
     1021 gccgtggcgg acacgtgcag gacctgagtg ccgccgacga cgatccaggc cgcacgcaga
     1081 gccggttgtc gctcaagcgg ccggcctgaa cctggcgacg cccgctgcgg cggggaacgc
     1141 ccgctgcggc gcgcggacgc gccgcggttc cgcggcggcg ccggctactt gcgaaccgat
     1201 ttcttcgccg cggtcttgcg cttgggcttc ttgggccgcg acttgccgaa ggtaccggcg
     1261 tagatcttgc cgcgacgcgt cttgcgatca cccttgccca ctgctgctgc tcccggtgct
     1321 gaggaagggc cggaagtcta gagactcgcg ccttgcggcg caaacccgca cagcgcgcga
     1381 acggtgacct ggagccaggc gccggcctag cggaagcgcg ccagtgtctt cgcgagtgcg
     1441 gccgcgccgg ggttgaggcg ctcgtaggga atctcgttaa gcgcctcgtc gggcgtgacg
     1501 ttgagctcgt ggaattccgg ctgtcccgcg ctgatgtgca ggagcccggt caggtattcc
     1561 tgcgcgcgca gccgctcgcg gatgcgcgcc agcgcggcgc cacggtcggt cgggtcgtaa
     1621 tccggatcca ccttgcgcag cacgatgcgg ctgccgtcgt gcatcgtcac cggcacgctt
     1681 tcgcccggcg cgtactggac gctgatctcg gccgccggcg ccaccaggtc ggcgtgcacc
     1741 acttcttcgt agtggtcacg gctgtactgg tagctgcgcg tcgagccctc gtggtcgttg
     1801 aaggtcacgc agggcgagag cacgtcgatg agcgcgaagc cggagtgatg catggccgcc
     1861 tggatcagcg gcacgagctg cgccttgtcg cccgagaagc tgcgtgccac gaacgaggcg
     1921 ccgagcgtga gcgcgagttg caccggatcg atcggcgact ggttgttgac ctcgcccttc
     1981 ttggcggtgc tgccgatgtc ggccgaggcc gagaattgcc ccttcgtgag gccgtagacg
     2041 ccgttgttct cgatgatgta gaccatgttg aggttgcggc ggatcgcgtg aatga
//
LOCUS       DASRDG010000195         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1540859, whole genome shotgun sequence.
ACCESSION   DASRDG010000195 DASRDG010000000
VERSION     DASRDG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540859"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFA35_03205"
     CDS             <1..798
                     /locus_tag="VFA35_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZT55207.1"
                     /translation="QISVAIRVDAAQRSAEIDFTGTSPQLVNNFNAPTAVCMAAVLYV
                     FRTLVDDDIPLNAGCLKPLRVIIPDGSMLNPHPPASVVAGNVETSTCITNALYGALGS
                     MAAGQCTMNNFTFGNARYQYYETISGGSGAGPGFDGTSVVQTHMTNSRLTDPEVLEFR
                     FPVRLDSYEIRKGSGGAGRYRGGDGGVRRVRFLEPMTASILSNGRRHGAFGMAGGAAG
                     QPGINRVERADGTREALDHIGSVEMAPGDVFVIETPGGGGFGVKSPP"
     gene            902..1207
                     /locus_tag="VFA35_03210"
     CDS             902..1207
                     /locus_tag="VFA35_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HZT55208.1"
                     /translation="MNTRLIASGALASVLAMGALSAAQAADPSKEKEKCYGVAKAGTN
                     DCANLSGTHSCAGQSKNAMALEDFNYVAKGSCAKMGGKSEDEAKAAIAAMAPEPMKK"
     gene            1207..2094
                     /locus_tag="VFA35_03215"
     CDS             1207..2094
                     /locus_tag="VFA35_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HZT55209.1"
                     /translation="MNPAARAGSGPGQPPVPAGIGLRQPHYAALFETTPALGFVEVHA
                     ENFFADGGAALAVLERAREHYPVSLHGVGLALGSAAGLDPWHLDRLARLAQRIEPARV
                     SDHCCFARAPLQAGAPVVHANDLLPIAFTDASLDIMARNVQQVQERLGRALLVENLAA
                     YVSFADDSIGEPAFFAQLCARTGCGMLLDLNNLMVNALNAGAPDPLAACCAFVDALPR
                     GSVAEIHLAGYAQTDSIAIDDHGSRVRPAVWALYEHALRRFGAALPTLVEWDTALPAF
                     EVLLAEAAQAGSIAARLSV"
     gene            2091..2876
                     /locus_tag="VFA35_03220"
     CDS             2091..2876
                     /locus_tag="VFA35_03220"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HZT55210.1"
                     /translation="MNEAARQQRLLQALAGAAPHDAAALGLRESGARAARGLEAYRAN
                     AEGVADRALASTFPTIRAMVGADDFARVARAFWHAQPPRRGDLGEWGDAFAGWLAADA
                     ALARWPWLADCARLDWALHACERAADAVLDAASLGQLESTDPAQLRLRLMPGAALLRS
                     AWPLAAIHRAHQLEGAAAEAAFDELRAAIAAARGEPVFVARRGWRAVVHPLDAPGAAW
                     MQDLLDGVDLAAALERAGPGFDFAAWLASALRESWLQGVSRAA"
     gene            complement(2860..>3635)
                     /gene="tolA"
                     /locus_tag="VFA35_03225"
     CDS             complement(2860..>3635)
                     /gene="tolA"
                     /locus_tag="VFA35_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HZT55211.1"
                     /translation="FGVNWRSDEPATVAAELWSATPQAAAPRAVEPPPPEVQKVEPVV
                     KPAPKPPEPVVAKPPAPALPDPQIAIEKAKKEAAKRAATEQEEAKKLKREKEELALKR
                     ERDKEKEKAEKLQAEQAKAEQAKREKAEKAEAARLAAIRDANLKRMAGLAGATGGATD
                     TGSALRSSGPSASYAGRVAARVKPNIVFTDTVDGNPQAVVQVRLAPDGTIVGKRLVQS
                     SGSKAWDEAVLRAIERTEILPLDNGRAPAPFEIGFRPRD"
BASE COUNT          437 a         1368 c         1297 g          533 t
ORIGIN      
        1 cagatcagcg tcgcgatccg cgtcgacgcg gcgcagcgca gcgccgagat cgatttcacc
       61 ggcacctcgc cgcaactcgt caacaacttc aacgcgccga cggccgtgtg catggccgcg
      121 gtgctgtacg tgttccgcac gctggtcgac gacgacatcc cgctgaacgc cggttgcctg
      181 aagccgctgc gggtcatcat ccccgacggc tcgatgctga acccgcaccc gccggcctcg
      241 gtggtggccg gcaacgtcga gacctcgacc tgcatcacca acgcgctgta cggcgcgctc
      301 ggcagcatgg ccgcgggcca gtgcacgatg aacaacttca ccttcggcaa tgcgcgctac
      361 cagtactacg agaccatttc cggaggctct ggtgccgggc cgggcttcga cggcacgagc
      421 gtcgtgcaga cccacatgac gaactcgcgc ctcaccgacc ccgaggtgct cgagttccgc
      481 ttcccggtgc ggctcgacag ctacgagatc cgcaagggct cgggcggcgc tggccgctac
      541 cgcggcggcg atggcggcgt gcggcgcgtg cgctttctcg agccgatgac cgcgtcgatc
      601 ctgtcgaacg ggcgccgcca cggcgcgttc ggcatggccg gcggcgcggc cggccagccg
      661 ggcatcaacc gcgtcgagcg cgccgacggc acgcgcgagg cgctggatca catcggctcg
      721 gtcgagatgg cgccgggcga cgtgttcgtg atcgagacgc cgggcggcgg cgggttcggc
      781 gtgaaatccc cgccgtaaga aaggacaggg cgtttcgaca gaagcgcatc gccgatcgag
      841 aatggcgagc cggccgaccg cctgttccgc aacccttcag ccccaccgga gaaccacgcc
      901 catgaacacc cgcctcatcg cctccggcgc cctcgcttcc gtgctcgcca tgggcgcgct
      961 ctccgctgcc caggccgccg acccgagcaa ggaaaaagag aagtgctacg gcgtcgccaa
     1021 ggccggcacc aacgactgcg ccaacctctc gggcacgcac tcgtgcgccg gccagtcgaa
     1081 gaacgcgatg gcgctcgaag acttcaacta cgtcgccaag ggcagctgcg cgaagatggg
     1141 cggcaagagc gaagacgaag ccaaggccgc gatcgcggcg atggcgccgg agccgatgaa
     1201 gaagtgatga acccggcggc gcgcgccggc agcgggccgg gccagccgcc cgtgccggcc
     1261 ggcatcggcc tgcgccagcc acactacgcg gcgctgttcg agaccacccc ggcgctcggc
     1321 ttcgtcgagg tccacgccga gaatttcttc gccgacggcg gcgccgcgct cgcggtgctc
     1381 gaacgtgcgc gcgagcacta cccggtcagc ctgcacggcg tcggcctcgc gctgggctcg
     1441 gccgcggggc tcgacccctg gcacctcgac cggctcgcgc ggctcgcgca gcgcatcgag
     1501 ccggcgcgcg tctccgacca ctgctgcttc gcgcgcgcgc cgctgcaggc cggcgcgccc
     1561 gtcgtgcatg cgaacgacct gctgccgatc gcgttcaccg acgcgtcgct cgacatcatg
     1621 gcgcgcaacg tgcagcaggt gcaggagcgg ctgggccgcg cgctgctggt cgagaacctg
     1681 gcggcctacg tctcgttcgc cgacgactcg atcggcgagc ccgcgttctt cgcgcagctc
     1741 tgcgcgcgca ccggctgcgg catgctgctc gacctcaaca acctgatggt caacgcgttg
     1801 aacgccggcg cgcccgatcc gctcgcggcg tgctgcgcgt tcgtcgatgc gctgccgcgc
     1861 ggcagcgtcg ccgagatcca tctcgccggc tacgcgcaga ccgattcgat cgcgatcgac
     1921 gaccacggca gccgcgtccg cccggccgtg tgggcgctgt acgaacatgc gctgcgccgc
     1981 ttcggcgccg cgctgccgac gctggtcgag tgggacaccg cgctgcccgc gttcgaggtg
     2041 ctgctcgccg aggccgcgca ggccgggtcg atcgcggcgc ggctctccgt atgaacgagg
     2101 ccgcacgcca gcagcgcctg ctccaggccc tggccggcgc cgcgccgcac gacgccgcgg
     2161 cgctcgggct gcgcgagtcg ggcgcgcgcg ccgcgcgcgg gctcgaggcc tatcgcgcca
     2221 atgccgaggg cgtcgccgac cgcgcgctgg cgtcgacctt cccgaccatt cgggcgatgg
     2281 tcggcgcgga cgacttcgcg cgcgtcgcgc gcgcgttctg gcacgcgcag ccgccgcggc
     2341 gcggcgacct cggcgaatgg ggcgacgcgt tcgccggctg gctcgcggcc gatgccgcgc
     2401 tcgcgcgctg gccctggctc gccgactgcg cgcggctcga ctgggcgctg cacgcctgcg
     2461 agcgcgccgc cgacgccgtg ctcgacgcgg cgtcgctggg ccagctggaa tcgaccgacc
     2521 cggcgcagct gcggctgcgg ctgatgcccg gcgccgcgct gctgcgttcg gcctggccgc
     2581 tcgcggcgat ccatcgcgcg caccagctcg aaggcgcggc ggccgaggcc gcgttcgacg
     2641 agctgcgcgc cgcgatcgcg gccgctcgcg gcgagccggt gttcgtcgcg cggcgtggct
     2701 ggcgcgccgt cgtgcatccg ctcgatgcgc ccggcgcggc gtggatgcag gacctgctcg
     2761 acggcgtcga cctcgccgcg gcgctggagc gcgccggccc gggcttcgac ttcgccgcct
     2821 ggctcgcgag cgcgctgcgc gagtcgtggc tgcagggcgt cagtcgcgcg gcctgaagcc
     2881 gatctcgaac ggcgcgggcg cgcggccgtt gtccagcggc aggatctcgg tgcgctcgat
     2941 cgcgcgcagc accgcctcgt cccaggcctt gctgccgctg ctttgcacca ggcgcttgcc
     3001 gacgatcgtg ccgtccggtg cgagccgcac ctgcacgacc gcctgcgggt tgccgtcgac
     3061 cgtgtcggtg aagacgatgt tcggcttgac ccgcgcggcg acgcggccgg cgtagctggc
     3121 cgacggcccg ctgctgcgca gcgcgctgcc ggtgtcggtc gcgccgccgg tcgcgccggc
     3181 gaggccggcc atgcgcttga ggttcgcgtc gcggatcgcg gcaaggcgcg cggcctcggc
     3241 cttctcggcc ttctcgcgct tcgcctgttc ggccttggcc tgctccgcct ggagcttctc
     3301 ggccttctct ttctccttgt ccctttccct cttcagcgcg agttcctcct tctcgcgctt
     3361 gagtttcttc gcctcttcct gctcggtggc ggcgcgcttc gccgcctcct tcttcgcctt
     3421 ctcgatcgcg atctgcgggt cgggcagcgc cggcgcgggc ggtttggcga cgaccggctc
     3481 gggcggtttg ggcgcgggct tgacgaccgg ttcgaccttc tgcacctcgg gcggcggcgg
     3541 ctcgaccgcg cgcggcgcgg cggcctgcgg cgtcgccgac cagagttcgg cggcgaccgt
     3601 cgcgggctcg tcgctgcgcc agttcacgcc gaagg
//
LOCUS       DASRDG010000196         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_484568, whole genome shotgun sequence.
ACCESSION   DASRDG010000196 DASRDG010000000
VERSION     DASRDG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484568"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..854
                     /locus_tag="VFA35_03230"
     CDS             150..854
                     /locus_tag="VFA35_03230"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZT55212.1"
                     /translation="MSLQDRPGQGCIMSDEDFSESRQRMLALIAAKTIYSCAHLGKAA
                     LSPRVMAALAKVPRHEFVPLEMQPHAYADTPLPVGFGKTISQPFIVAVMTDLLDLQPT
                     DTVLEIGTGLGYQTAVLAGLVRRVYSVELIEELASQAIHRLARQHCANVEIKVGNGWH
                     GWPEHAPFDKVIVTAAPELIPPSLIYQLKPGGKMVIPAGLPDAQQLIVLDKDAGDTVS
                     TREIFAVQFSLLDDDS"
     gene            complement(870..1652)
                     /locus_tag="VFA35_03235"
     CDS             complement(870..1652)
                     /locus_tag="VFA35_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZT55213.1"
                     /translation="MSFDVGFGLVGALLPLLLLVAASLRILREYERGVVFQLGRFWKV
                     KGPGLVILVPGIQQMVRVGLRVVVLDIPSQDVITRDNVSVKVNAVLYFRVVDPERAII
                     QVENYLAATSQLAQTTLRAVLGKHDLDDLLAERERLNLDIQKTLAAQTDAWGIKVTIV
                     EIKHVDLNDSMVRAIARQAEAERERRAKVIHAEGELQASVKLSEAAEILERHPQALQL
                     RYLETLTVIAADKNSTIVFPLPLDIIGPLLRLLDRRPASPGE"
     gene            complement(1649..>2371)
                     /locus_tag="VFA35_03240"
     CDS             complement(1649..>2371)
                     /locus_tag="VFA35_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZT55214.1"
                     /translation="GREIVLAGATLRLATAGVPVVEFEADWRSRFLSVITDPSLALIL
                     MLVGIYGLLFEFANPGFVLPGVVGGISLLLALFALQMLPVNYAGLALILLGVAFLIAE
                     AFLPTFGALGVGGIVAFAFGAVLLFDVDVPGFGIPKALIALLSATSAVFILGVAGMAA
                     KARRRPVMNMLRGSPALLGARGELIEFSGGEGWAMIRGEHWKVHGAGDLQAGRQVRVT
                     RAQGQALEVAADGADLSAGVPS"
BASE COUNT          417 a          839 c          763 g          352 t
ORIGIN      
        1 atcggcgcgc tcgcgggctg ttctactctg actcacagtg tgctcccgga tcggccgcaa
       61 tgccagaccg tgctgcgttt gccgccggca tggacgccgc ccgaagcgta ggatggatgt
      121 ggccgtgtgg cctcgcacga aggcgcgccg tgagcctgca ggaccggccg ggccaggggt
      181 gcatcatgag cgacgaagac ttctcggaat cacgccagcg catgctcgcg ctcatcgccg
      241 ccaagacgat ctattcgtgc gcccacctgg gcaaggcggc gctgagcccg cgggtcatgg
      301 cggcgctggc caaggtaccg cgccacgagt tcgtgccgct ggagatgcag ccccacgcct
      361 acgccgacac gcccttaccg gtcgggttcg gcaagaccat ctcgcagccg ttcatcgtcg
      421 ccgtgatgac cgacctgctc gacctccagc ccaccgacac ggtgctggag atcggcaccg
      481 ggctgggcta ccagacggcg gtgctggcag ggctcgtgcg gcgggtctac agcgtggagc
      541 tgatcgaaga gctggcctcg caagccattc accggctggc tcggcaacac tgcgccaacg
      601 tcgagatcaa ggtcggcaac ggctggcacg gctggcccga acatgcgccg ttcgacaagg
      661 tgatcgtgac ggcggcgccc gagctgatcc cgccctcgct gatctaccag ctgaagcccg
      721 gcggaaagat ggtgatccct gcgggcctgc ccgacgcaca gcaactcatc gtgctcgaca
      781 aggacgccgg cgatacggtg tcgacccggg agatctttgc cgtgcagttc tcgctgctcg
      841 acgacgacag ttgagtcgcg cagagggcgc tattcgccgg gcgacgccgg cctgcggtcg
      901 agcaggcgca gtagcggccc gatgatgtcc agcggcaacg gaaacacgat ggtcgagttc
      961 ttgtcggcgg caatcaccgt cagcgtctcc agatagcgca gttgcaaggc ctgcgggtgg
     1021 cgctcgagaa tctcggccgc ctccgacaac ttcacggagg cctgcagttc gccctcggca
     1081 tggatcacct tggcgcgtcg ctcgcgctcg gcctcggcct gccgggcgat ggcccgtacc
     1141 atgctgtcgt tcaggtcgac atgcttgatc tcgacgatgg tcaccttgat gccccaggca
     1201 tcggtctgcg ccgccagggt cttctggatg tccaggttca gccgctcgcg ctcggccagc
     1261 aggtcgtcga ggtcgtgctt gcccagcacc gcgcgcagcg tggtctgcgc gagctggctg
     1321 gtggccgcga ggtagttctc gacctggatg atggcgcgct cgggatcgac gacgcgaaag
     1381 tacagcaccg cgttgacctt caccgaaacg ttgtcgcgcg tgatcacgtc ctggctcggg
     1441 atgtccagta ccaccacgcg caggcccact cgcaccatct gctggatgcc cggaacgagg
     1501 atcaccagcc ccggcccctt gaccttccag aatcggccga gctggaacac cacgccgcgt
     1561 tcgtactcgc gcaagatgcg cagcgacgcc gccaccagga gcagcagcgg caacagcgcg
     1621 cccaccaacc cgaacccgac atcgaagctc atgatggcac ccccgcgctg aggtcggcgc
     1681 catctgcagc gacctccaat gcctggcctt gggcgcgggt cacgcgcacc tgacggcccg
     1741 cttgcaggtc gcctgcgccg tgcaccttcc agtgctcgcc acggatcatg gcccagcctt
     1801 cgccgcccga gaactcgatc agttcgcccc tcgcgccgag cagggcgggg ctgccacgca
     1861 gcatgttcat gaccgggcgc cgccgggcct tcgcggccat gcccgcgacg ccaagaatga
     1921 acaccgccga ggtcgcgctc agcagcgcga tcagcgcttt cgggatgccg aagcccggca
     1981 catcgacgtc gaacagcagc accgcaccga acgcgaacgc gacgatgcca cccaccccca
     2041 gcgcaccgaa ggtcggcagg aaggcttcgg cgatcaggaa cgccacgccg agcaggatca
     2101 gcgccagccc cgcgtagttg accggcagca tctgcaacgc gaacagggcc agcagcaggc
     2161 tgatgccgcc caccacaccc ggcagcacga aacccgggtt ggcgaactcg aacaacaagc
     2221 cgtagatgcc cacgagcatc aggatcaggg ccaggctcgg gtcggtgatc actgacaaga
     2281 accggctgcg ccagtcggcc tcgaactcga ccaccggcac gcccgccgtg gccagcctga
     2341 gcgttgcgcc ggcgagcacg atctcgcgcc c
//
LOCUS       DASRDG010000197         8105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_260721, whole genome shotgun sequence.
ACCESSION   DASRDG010000197 DASRDG010000000
VERSION     DASRDG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260721"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1528
                     /locus_tag="VFA35_03245"
     CDS             68..1528
                     /locus_tag="VFA35_03245"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZT55215.1"
                     /translation="MLKLKDPGLFSQQAYIAGEWVDADAGATVAVTNPATGEQLGTVP
                     MCGTAETARAIAAADVAQRAWRDVPAKERAAILRKLNDLMLANADDLALIMTSEQGKP
                     LAEAKGEIAYAASFIEWFADEARRIYGDTIPATSADKRIVAIKQPVGTTAAITPWNFP
                     TAMLTRKAGPALAAGCSMVVKPATQTPYSALAFAVLAERAGVPKGLLAVLTGSASQIG
                     GEMTRNPLVRKLSFTGSTEVGRTLMRQSADTIKKLSLELGGNAPFIVFDDADLDAAVD
                     GAMISKYRNTGQTCVCANRIYVQRGVAKAFTEKLVAKVKGLKVGNGLDAGVTQGPLID
                     AKAVAKIEEHVADARAKGGEVLTGGKRHALGGLFFEPTVIGGATAEMLFAHEETFGPL
                     APIFAFDTEAEVLALANDSEFGLAAYFYSRDIGRVMRVAERLESGIVGINTGLISTAE
                     APFGGVKQSGLGREGSKYGLDDYLEIKYLCFGGLDK"
     gene            1525..2397
                     /locus_tag="VFA35_03250"
     CDS             1525..2397
                     /locus_tag="VFA35_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZT55216.1"
                     /translation="MSVPLASQELFGIAGVQMNVSAFEENVDKMGAYLRHIRTRFPWV
                     RMVLFSELAPLGPKHAKAEALPGPTEARLCALARETGLWLIPGSLFERVDGVVYNTTP
                     VIDPRGEVIARFRKLFPFRPYERDVAGGKEFCVFDVPGAGRFGVSICYDMWFPETTRT
                     LVAMGAEVILHPTMTDTIDRDVELSIARASAVTNQVYFFDVNGVGDGGVGRSIICDPS
                     GYVLHQADGGAEIMPIEVDFARVRRERERGLRSNLGQPLKSFRDRECDFDVYRRDRGG
                     DAFLNTLGPLTKPD"
     gene            2467..5334
                     /locus_tag="VFA35_03255"
     CDS             2467..5334
                     /locus_tag="VFA35_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZT55217.1"
                     /translation="MAEKKSAVEARASADSLSRHRSVWEMRADGWISLVDDLGRLATL
                     PRDHADWAACFANVRRIVTALAPIESYWAFPGGRVFGELGTWIDRGELARAHQAARRI
                     HRMLAAQTYRHETSALDSEGELPSQIESDSERQAQLSRPYFEVLIVDEMTASEEDALR
                     RRVQHKRNPDDDFIFDVVIVPSFEDALIATLVNFNLQAVVIRHGFPFRSMYHNDMLRH
                     FVDGVDNSIEALPESERGPMLGRQIAQLRPELDLYLVTDVNVEEIAARAGELFKRIFF
                     REEDHIELYGSIMKGVHERHHTPFFSALREYAKQPTGVFHALPLARGKSIMNSNWIGD
                     LAQFYGMNLFMAETSATSGGLDSLLDPVGPLKVAQEYAARAFGAKRTYFATNGTSTCN
                     KIVVQALVRPDDIVLVDRNCHKSHHYGMVLAGAQVTYLDSYPLDQFSMYGAVPVRHIK
                     ETLLSFRKAGTLDRVRMVLLTNCTFDGLVYDVERVMMECLAIKPDLIFLWDEAWFAFA
                     RCHPIYRQRTGMATAARLSQTFADPAYAQRYAEFAKGFDADAWNDDERVLNTRLLPDP
                     AKARIRVYATHSTHKTLTALRQGSMIHVWDQDFREKAEEAFHEAYMTHTSTSPNYQIL
                     ASLDVGRRQVELEGYELVQRQLELAMSLREQVLNHPLLKRYFRFLRVGDLVPAQYRES
                     GVDNYYNIETGWNNLEAAWRTDEFALDPTRATLYIGLTGMDGDTFKNKELMDRYGIQI
                     NKTSRNTVLFMTNIGSTRSAVAYLLEVLVKIARDVDQRVADMSALERRIHDKRVRSLT
                     LELPPLPDFSSFHFAFRGRGVEGRVETRDGDIRSAFFLSYDEANCDYIGMQEAQQAVK
                     GGRELVSALFVIPYPPGFPILVPGQVISAEILQFMAALDVREIHGFRPELGFRIFTDV
                     ALERVAQATAARAAVALAGRAAQPQASDAKAAKSPSESKVS"
     gene            5370..6830
                     /locus_tag="VFA35_03260"
     CDS             5370..6830
                     /locus_tag="VFA35_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HZT55218.1"
                     /translation="MSTNKVKSISDLLLMFHRNERPIYFISATNFNLLGMDRWVNRFR
                     YISYIDCFDGRHPNVFVPSETPHAEFESIEDIVNHLLQHKEVIDMIERRGGKPAATFL
                     MFDEKTEALAKEIGLDIWFPPAKLRQRCDNKMETVRIGNKAGVFSVPNALEKVESYAA
                     LRQICDRAGLGHDLVIQSAFGDSGHTTFFIANEDDFNKHKDEIVNDPEVKIMKRINCR
                     GATLEACATQSGTLVGPLLTEVVGAKELTPYKGGWCGNEVFPGAFSEEVRAKARAMAE
                     KFGNQLLAEGYRGYFDLDFLIDQDNGEVYLGELNPRVCGASPMTNHAAFAYADAPLFL
                     FHLLEFSGVPFDLNVKELNERWAQPHFIDSWSQVVIKYTEDKVDQVTRAPATGIYRMA
                     EDGSVGYQRFDYNRKAIDSEREAFFMRITGPGDYRYEGADLGILITRGRSMNDDFKLN
                     LRARDWIRGIKQHYDGKPIVTTQHEAAPAPGSFKIL"
     gene            6847..7899
                     /locus_tag="VFA35_03265"
     CDS             6847..7899
                     /locus_tag="VFA35_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HZT55219.1"
                     /translation="MAIQVTFATLLEERPGAAWQAVFERLWPGYRAWFLRSGTVDRPS
                     YLQCRRALREHMPELVPTWERLVELAGGGDLEARFLSLWCPPRYITGCSQAVWLDPAG
                     AAEPALLRNYDFAPALLEGNWLATNWLGPRVVAMGDCLWGALDGINEAGLAASLSFGG
                     RTVSGAGFGIPLVLRYLLEVAQNTAEAIALLQRVPVSMSYTVTLLDARAEWATVFVAP
                     DRAVEVTRHRAVTNFQHRVEWPEHARATRAQERLACLQQQMDAAAPLPAAIDALLQPP
                     LFQSAWLRGYGTLYSAVYRPRSGGVELLWPGERWAQSVDDFEEGRREIVYAPGGYALA
                     GQEDHAVMQVLAESRR"
BASE COUNT         1365 a         2807 c         2714 g         1219 t
ORIGIN      
        1 atgccgtgtt cgaggaaggc ctggccattc tcgccaacgc gctcgaagcc tgatccggaa
       61 gatgccgatg ctcaagctca aagaccccgg cctgttcagc cagcaggcct acattgccgg
      121 cgaatgggtc gatgccgacg ccggcgccac ggtggccgtc accaacccgg ccaccggcga
      181 acaactcggc accgtgccga tgtgcggcac ggccgagacc gcgcgcgcga ttgccgccgc
      241 cgatgtggcc cagcgcgcct ggcgcgacgt gccggcgaag gagcgcgccg cgatcctgcg
      301 caagctcaac gacctgatgc tcgcgaacgc cgacgacctg gcgctgatca tgaccagcga
      361 gcagggcaag ccgctggccg aagcgaaggg cgagatcgcg tacgccgcgt cgttcatcga
      421 gtggttcgcc gacgaggcgc gccgcatcta cggcgacacg attccggcga cctcggccga
      481 caagcgcatc gtcgcgatca agcagccggt gggcacgacc gcggcgatca cgccgtggaa
      541 cttccccacc gcgatgctga cgcgcaaggc cggcccggcg ctggccgcgg gctgctcgat
      601 ggtcgtcaag ccggcgacgc agacgccgta ctcggcgctc gcgttcgcgg tgctggccga
      661 gcgcgccggc gtgcccaagg gcctgctcgc ggtgctgacc ggctcggcca gccagatcgg
      721 cggcgagatg acacgcaacc cgctggtgcg caagctcagc ttcaccggct cgaccgaggt
      781 cggccgcacg ctgatgcggc agagcgccga cacgatcaag aagctctcgc tcgagctcgg
      841 cggcaacgcg ccgttcatcg tgttcgacga tgccgatctc gatgccgcgg tcgacggcgc
      901 gatgatcagc aagtaccgca acaccggcca gacctgcgtc tgcgcgaacc gcatctacgt
      961 gcagcgcggc gtcgcgaagg cgttcaccga aaagctggtc gcgaaggtga agggcctgaa
     1021 agtcggcaac ggcctggacg ccggcgtcac gcaaggcccg ctgatcgacg cgaaggcggt
     1081 cgcgaagatc gaggagcacg tcgccgatgc ccgcgccaag ggcggcgagg tgctgaccgg
     1141 cggcaagcgc cacgcgctcg gcggcctgtt cttcgagccg accgtgatcg gcggcgcgac
     1201 cgccgagatg ctgttcgcgc acgaggagac cttcggcccg ctcgcgccga tcttcgcgtt
     1261 cgacaccgag gccgaggttc tcgcgctcgc gaacgactcc gagttcggcc tggccgcgta
     1321 tttctacagc cgcgacatcg gccgcgtgat gcgcgtcgcc gaacggctcg aatcgggcat
     1381 cgtcggcatc aacaccgggc tgatctcgac cgccgaggcg ccgttcggcg gcgtcaagca
     1441 gtcgggcctg ggccgcgaag gctcgaagta cgggctcgac gactacctcg aaatcaagta
     1501 cctgtgcttc gggggtctcg acaagtgagt gtcccgctcg cgagccagga gctgttcggc
     1561 atcgccgggg tgcagatgaa cgtctcggcg ttcgaggaga acgtcgacaa gatgggcgcc
     1621 tacctgcgcc acatccgcac ccgcttcccg tgggtgcgga tggtgctgtt ctcggaactg
     1681 gcgccgctcg gccccaagca cgcgaaggcc gaggcgctgc cggggccgac cgaggcgcgc
     1741 ctgtgcgcgc tggcgcgtga aaccgggctg tggctgatcc cgggctcgct gttcgagcgg
     1801 gtcgacgggg tggtctacaa caccacgccg gtgatcgacc cgcgcggcga ggtgatcgcg
     1861 cgctttcgca agctgttccc gtttcgcccc tacgagcgcg acgtcgccgg cggcaaggag
     1921 ttctgcgtct tcgacgtgcc cggcgccggc cgcttcggcg tctcgatctg ctacgacatg
     1981 tggttcccgg agaccacgcg cacgctggtc gcgatgggcg ccgaggtgat cctgcacccg
     2041 acgatgaccg acacgatcga ccgcgatgtc gagctgtcga tcgcgcgtgc ctcggcggtg
     2101 acgaaccagg tctacttctt cgatgtcaac ggcgtcggcg acggcggcgt cggccgctcg
     2161 atcatctgcg acccgtcggg ctacgtgctg caccaggccg acggcggtgc cgagatcatg
     2221 ccgatcgagg tcgatttcgc acgcgtgcgg cgcgagcgcg agcgcgggct gcgcagcaac
     2281 ctgggccagc cgctgaagag ctttcgcgat cgtgaatgcg atttcgacgt ctaccggcgc
     2341 gaccgcggcg gcgacgcctt tctgaacacc ttggggcccc tgaccaaacc cgattgaccg
     2401 cgcccgccgg gcgccaacac aaccgaagcc gggtgccatg gagccccggc tcgctggaga
     2461 tgagacatgg ccgagaagaa gtccgccgtc gaggcgcgcg cgagcgctga ttcgctgagc
     2521 cgtcaccgca gcgtctggga gatgcgcgcc gatggctgga tcagcctggt cgacgacctg
     2581 ggccgcctgg cgacgctgcc gcgcgaccac gcagactggg cggcgtgctt cgcgaacgtg
     2641 cgccgcatcg tcaccgcgct ggcgccgatc gagagctact gggccttccc gggcggccgc
     2701 gtgttcggcg agctcggcac ctggatcgac cgcggcgagc tcgcccgcgc gcaccaggcg
     2761 gcgcggcgca tccaccgcat gctcgcggcg cagacctacc gccacgagac cagcgcgctc
     2821 gacagcgaag gcgagttgcc gtcgcagatc gagagcgaca gcgaacgcca ggcccagctg
     2881 tcgcgcccgt acttcgaggt gctgatcgtc gacgagatga cggccagcga agaagacgcg
     2941 ctgcgccggc gcgtgcagca caagcgcaac cccgacgacg acttcatctt cgatgtcgtg
     3001 atcgtgccga gcttcgaaga cgcgctgatc gccacgctgg tcaacttcaa cctgcaggcg
     3061 gtcgtgatcc gccacggctt cccgttccgc tcgatgtacc acaacgacat gctgcggcac
     3121 ttcgtcgacg gcgtggacaa cagcatcgag gcgctgcccg aatccgaacg cggcccgatg
     3181 ctgggccggc agatcgcgca gctgcgtccc gagctcgatc tgtacctcgt gaccgacgtc
     3241 aacgtcgaag agatcgccgc gcgcgccggc gagctgttca agcgcatctt cttccgcgag
     3301 gaggaccaca tcgagctcta cggctcgatc atgaagggcg tgcacgagcg ccaccacacg
     3361 ccgttcttca gcgcgctgcg cgagtacgcg aagcagccca ccggcgtgtt ccacgcgctg
     3421 ccgctggcgc gcggcaagtc gatcatgaac tcgaactgga tcggcgacct ggcgcagttc
     3481 tacggcatga acctgttcat ggccgagacc tcggcgacct cgggcgggct cgattcgctg
     3541 ctcgacccgg tcggcccgct gaaggtggca caggagtacg cggcgcgcgc cttcggcgcg
     3601 aagcgcacct acttcgcgac caacggcacc agcacctgca acaagatcgt cgtgcaggcg
     3661 ctggtgcggc ccgacgacat cgtgctggtc gaccgcaact gccacaagtc gcaccactac
     3721 ggcatggtgc tcgcgggcgc gcaggtcacc tacctcgact cgtacccgct cgaccagttc
     3781 tcgatgtacg gcgcggtgcc ggtgcgccac atcaaggaga cgctgctgag tttccgcaag
     3841 gccggcacgc tcgaccgcgt gcgcatggtg ctgctgacca actgcacctt cgacggcctg
     3901 gtctacgacg tcgagcgcgt gatgatggaa tgcctggcga tcaagccgga cctgatcttc
     3961 ctgtgggacg aggcctggtt cgcgttcgcg cgctgccacc cgatctaccg ccagcgcacc
     4021 ggcatggcca ccgcggcgcg gctgagccag accttcgccg acccggccta tgcgcagcgc
     4081 tacgcagagt tcgcgaaagg cttcgacgcc gacgcctgga acgacgacga gcgcgtgctg
     4141 aacacgcggc tgctgcccga cccggccaag gcgcgcatcc gcgtctacgc gacccactcg
     4201 acgcacaaga cgctgaccgc gctgcgccag ggctcgatga tccatgtgtg ggaccaggac
     4261 ttccgcgaga aggctgagga ggccttccac gaggcctaca tgacccacac ctcgacctcg
     4321 ccgaactacc agatcctcgc gtcgctcgac gtcggccggc gccaggtcga gctcgaaggc
     4381 tacgagctgg tgcagcgcca gctcgagctc gcgatgagcc tgcgcgagca ggtgctgaac
     4441 cacccgctgc tcaagcgcta cttccgcttc ctgcgcgtcg gcgatctggt gccggcgcag
     4501 taccgcgagt cgggcgtcga caactactac aacatcgaga ccggctggaa caacctcgag
     4561 gccgcctggc gcaccgacga gttcgcgctc gacccgacgc gcgcgacgct gtacatcggc
     4621 ctgaccggca tggacggcga cacgttcaag aacaaggagt tgatggaccg ctacggcatc
     4681 cagatcaaca agacctcgcg caacaccgtg ctgttcatga ccaacatcgg cagcacgcgc
     4741 tcggcggtgg cctacctgct cgaggtgctg gtgaagatcg cccgcgatgt cgaccagcgc
     4801 gtggccgaca tgagcgcgct cgagcgccgc atccacgaca agcgcgtgcg ttcgctgacg
     4861 ctcgagctgc cgccgctgcc cgacttctcg agcttccact tcgcgttccg cggccgcggc
     4921 gtcgagggtc gcgtcgagac gcgcgacggc gacatccgca gcgcgttctt cctctcctac
     4981 gacgaagcga actgcgacta catcggcatg caggaggctc agcaggccgt caagggcggg
     5041 cgcgagctgg tgtcggcgct gttcgtgatc ccgtacccgc cgggcttccc gatcctggtg
     5101 ccggggcagg tgatcagcgc cgagatcctg cagttcatgg ccgcgctcga cgtgcgcgag
     5161 atccacggct tccggcccga gctgggcttt cgcatcttca ccgacgtggc gctcgagcgc
     5221 gtcgcgcagg cgaccgcggc gcgcgccgcg gtggcgctcg ccggccgcgc cgcccagccg
     5281 caggcgagcg atgcgaaagc cgcgaagtcg ccgtccgaat ccaaagtgtc ctgatgtcct
     5341 gatgtcctga gcctccaagg aaaaccacga tgagcacgaa caaggtcaag agcatttccg
     5401 atctgctgct gatgttccac cgcaacgagc ggccgatcta cttcatcagc gccaccaact
     5461 tcaacctgct cggcatggac cgctgggtca accgtttccg ctacatcagc tacatcgact
     5521 gcttcgacgg ccgccacccg aacgtgttcg tgccgagcga gacgccgcat gccgagttcg
     5581 aatcgatcga ggacatcgtc aaccacctgc tgcagcacaa ggaggtcatc gacatgatcg
     5641 agcgccgcgg cggcaagccg gcggcgacct tcctgatgtt cgacgagaag accgaggcgc
     5701 tggccaagga gatcgggctg gacatctggt tcccgccggc caagctgcgc cagcgctgcg
     5761 acaacaagat ggagaccgtg cgcatcggca acaaggccgg cgtgttctcg gtgccgaacg
     5821 cgctcgagaa ggtcgagagc tacgccgcgc tgcgccagat ctgcgaccgc gccgggctcg
     5881 gccacgacct ggtgatccag tcggccttcg gcgactcggg ccacaccacg ttcttcatcg
     5941 cgaacgagga cgatttcaac aagcacaagg acgagatcgt caacgacccc gaggtcaaga
     6001 tcatgaagcg catcaactgc cgcggcgcga cgctggaagc gtgcgccacg cagtcgggca
     6061 ctctcgtcgg gccgctgctg accgaggtcg tcggcgccaa ggagctgacg ccgtacaagg
     6121 gcggctggtg cggcaacgag gtcttccccg gcgcgttctc cgaggaggtg cgcgccaagg
     6181 cgcgcgcgat ggccgagaag ttcggcaacc agctgctggc cgaaggctac cgcggctact
     6241 tcgacctcga cttcctgatc gaccaggaca acggcgaggt ctacctcggc gagctgaacc
     6301 cgcgcgtctg cggcgccagc ccgatgacca accacgccgc gttcgcgtat gccgacgcgc
     6361 cgctgttcct gttccacctg ctcgagttct cgggcgtgcc cttcgacctc aacgtcaagg
     6421 agctcaatga gcgctgggcc cagccgcact tcatcgacag ctggagccag gtcgtcatca
     6481 agtacaccga agacaaggtc gaccaggtca cgcgcgcgcc ggccaccggc atctaccgga
     6541 tggccgagga cggcagcgtc ggctaccagc gcttcgacta caaccgcaag gccatcgact
     6601 ccgagcgcga ggccttcttc atgcgcatca ccggccccgg cgactaccgc tacgagggcg
     6661 ccgacctcgg catcctgatc acgcgcgggc gctcgatgaa cgacgacttc aagctcaacc
     6721 tgcgcgcgcg cgactggatc cgcggcatca agcagcacta cgacggcaag ccgatcgtga
     6781 cgacgcagca cgaggctgcg ccggcaccgg gttcgttcaa gattctctag gccgcccgtc
     6841 gacgccatgg ccatccaggt cacgttcgcg acgctgctcg aagagcggcc gggcgcggcg
     6901 tggcaggcgg tgtttgagcg cctgtggccg gggtaccgcg cctggttcct gcgcagcggc
     6961 acggtcgacc ggccgagcta cctgcaatgc cggcgtgcgc tgcgcgagca catgcccgag
     7021 ctggtgccga cctgggagcg cctggtcgag ctcgccggcg gcggcgacct ggaagcgcgc
     7081 ttcctgtcgc tgtggtgccc gccgcgctac atcaccggtt gctcgcaggc cgtgtggctc
     7141 gatccggccg gcgcggccga gccggcgctg ctgcgcaact acgacttcgc gccggcgctg
     7201 ctcgaaggca actggctcgc gacgaactgg ctgggcccgc gcgtcgtcgc gatgggcgac
     7261 tgcctgtggg gcgcgctgga cggcatcaac gaagcggggc tggcggcgtc gctgtcgttc
     7321 ggcgggcgca ccgtctcggg cgccggcttc ggcatcccgc tggtgctgcg ctacctgctc
     7381 gaagtggcgc agaacaccgc cgaagccatt gcgctgctgc agcgcgtgcc ggtcagcatg
     7441 agctacaccg tcacgctgct cgacgcccgc gccgagtggg cgacggtgtt cgtcgcgccc
     7501 gaccgcgcgg tcgaggtcac gcggcaccgg gccgtcacca atttccagca ccgggtcgag
     7561 tggcccgagc atgcccgggc cacgcgcgcg caggagcggc tggcatgcct gcagcagcag
     7621 atggatgcgg ccgcgccgct gcccgcggcg atcgatgcct tgctgcagcc gccgctgttc
     7681 cagtcggcct ggctgcgcgg ctacggtacg ctctacagcg ccgtctatcg cccgcgcagc
     7741 ggcggcgtcg aactgctctg gcccggggag cgctgggcgc agtccgtcga tgatttcgaa
     7801 gaaggtcgac gcgagatcgt ctatgcgccg ggcggctatg cgctggccgg ccaggaggat
     7861 catgcggtga tgcaggtgct ggccgaatcc cggcgttgag tggtggcacg agtcgtgctc
     7921 ggtttccaag aagctcggtt tctcaaaaag gatgtcccat gaacagtcag acgaccaaga
     7981 agggcctgcc gccggcggcg tggatcatga tcgcgatggt gctcggcatc gtgatcggct
     8041 acatgatctt cacgagcttc cccgacaaga agacggcggc gcagatcgcc ggctacgtct
     8101 cgatc
//
LOCUS       DASRDG010000198         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_389912, whole genome shotgun sequence.
ACCESSION   DASRDG010000198 DASRDG010000000
VERSION     DASRDG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389912"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VFA35_03270"
     CDS             complement(<1..871)
                     /locus_tag="VFA35_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55220.1"
                     /translation="MTTLDQALQQFGALRQRARSGIWMEAAAMVLLAISGYALATWPI
                     DRHLRLEWPFRVVLLAGLAFGIVRILRRRLLRPMSVALDDDEMALAVERRAPDLRQAL
                     ISSLQFERALDGRGRGRIVDSTELMSAVIADVRGRLGAVPFAAAIDAARVRRFTALAA
                     ALLALFVAWGVVDGSGLRIWFLRNLCLASVEWPRYTQLSFVGATGNTVRLNQGDPLAV
                     HVAVAGVVPDQVFLRYEYDGGERGIEPMGRTGEGEFSTVMDSVLREALLVAEGGDGIT
                     EPLRVTIVERPRVD"
     gene            complement(868..>2248)
                     /locus_tag="VFA35_03275"
     CDS             complement(868..>2248)
                     /locus_tag="VFA35_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55221.1"
                     /translation="AVPERHQKSQAVEVLGERTRVLLLAGGASHEYQILRNLLIRDKT
                     IDVSCWLQNADPKFPQDGDDGVRIEALPADQKECDPYDVVILMDLDASKVTPGFCEMM
                     RKHIVENGCGMWWVCGEKNSLDAMRPTATTKPLADLLPVITDLQYAETVWSLGLAHVV
                     EYPYALTAEGEDGIAHKIARLVDGRDESRLLWSSLRFRVAFPVKQPKPAATVIAEHRL
                     ADRQLMHEGHGMPLIATQIVGAGRVLFSGMDETYRWRSIHEDAYDKFWVQGIRYLFEG
                     RLHAGDSRLRLLLSDEKVELGDAVTITVEAKNELLQPLIADSFELQLERDGQGAEVLK
                     LLPVEEVPGTFQLHGWRPTQVGAYRVRSPKRDGKQIEAGFQVVPARVEREGPMDRAEL
                     SAVAAANGGRLLDTPAQLLKALDEIPSRSATDTYRTPHPLWDGWGTVMFMLTALALEW
                     LLRKRFNLL"
BASE COUNT          323 a          825 c          774 g          326 t
ORIGIN      
        1 cgtcgacgcg cggccgctcg acgatcgtca cgcgcagcgg ctcggtgatg ccgtcgccgc
       61 cttcggcgac cagcagcgcc tcgcgcagca ccgagtccat caccgtcgag aactcgcctt
      121 cgcccgtgcg gcccatcggc tcgatgccgc gctcgccgcc gtcgtactcg tagcgcagga
      181 acacctggtc cggcacgacg cccgcgaccg cgacgtgcac cgccagcggg tcgccctggt
      241 tgagccgcac cgtgttgccg gtcgcaccga cgaacgacag ctgcgtgtag cgcggccatt
      301 cgacgctcgc gagacacagg ttgcgcagga accagatcct gagcccgctg ccgtccacga
      361 cgccccaggc gacgaacagc gccagcaggg cggccgcgag cgccgtgaac ctgcgcacgc
      421 gcgcggcgtc gatcgcggcc gcgaacggca ctgcgccgag ccggccgcgc acgtcggcga
      481 tcacggcgct catcagctcg gtcgaatcga cgatccgccc gcggccgcgg ccgtcgagcg
      541 cgcgctcgaa ctgcagcgag ctgatcagcg cctgccgcag atcgggcgcg cggcgttcga
      601 cggcgagcgc catctcgtcg tcgtcgaggg cgacggacat cggccgcagc aggcgccgcc
      661 gcaggatgcg cacgatgccg aacgcgagcc ccgcgagcag cacgacgcgg aacggccact
      721 cgaggcgcag gtggcggtcg atcggccagg tcgcgagcgc gtagccgctg atcgccagca
      781 gcaccatcgc cgcggcctcc atccagatcc ccgaccgcgc gcgctggcgc agcgcgccga
      841 actgttgcaa cgcctggtcg agcgtggtca tagcaggttg aacctcttgc gcagcagcca
      901 ctcgagcgcg agtgccgtga gcatgaacat gaccgtgccc cagccgtccc acagcgggtg
      961 cggcgtgcgg taggtgtcgg tggcgctgcg cgacgggatc tcgtcgagcg ccttcagcag
     1021 ctgcgccgga gtgtcgagca ggcggccgcc gttcgccgcg gcgacggcgc tgagctcggc
     1081 gcggtccatg gggccctcgc gctcgacgcg cgcgggcacc acctggaagc cggcttcgat
     1141 ctgcttgccg tcgcgcttgg gcgagcgcac gcggtaggcg ccgacctgcg tcggccgcca
     1201 accgtgcagc tggaacgtgc ccgggacctc ctcgaccggc agcagcttca gcacttcggc
     1261 gccctggcca tcgcgttcga gctgcagctc gaacgaatcg gcgatcagcg gctgcagcag
     1321 ctcgttcttc gcttcgacgg tgatcgtcac cgcgtcgccg agctcgacct tctcgtcgct
     1381 cagcagcagg cgcaggcggc tgtcgccggc gtgcagacgg ccctcgaaca ggtagcggat
     1441 gccctgcacc cagaacttgt cgtaggcgtc ctcgtggatc gagcgccagc ggtaggtctc
     1501 gtccatgccg ctgaacagca cgcggccggc gccgacgatc tgcgtggcga tcagcggcat
     1561 gccgtggcct tcgtgcatca gctgccggtc ggcgagccgg tgctcggcga tcaccgtcgc
     1621 cgcgggcttc ggctgcttga cggggaacgc gacgcggaag cgcagcgagc tccacagcag
     1681 ccggctctcg tccctgccgt cgacgagccg cgcgatcttg tgcgcgatgc cgtcctcgcc
     1741 ctcggccgtc agcgcgtacg gatactcgac gacgtgcgcg aggccgagcg accacaccgt
     1801 ctccgcgtac tgcagatcgg tgatcacggg cagcaggtcg gcgagcggct tggtggtcgc
     1861 cgtcggccgc atcgcatcga gcgagttctt ctcgccgcag acccaccaca tgccgcagcc
     1921 gttctcgacg atgtgcttgc gcatcatctc gcagaagccg ggcgtgacct tcgacgcgtc
     1981 gaggtccatc aggatcacga cgtcgtacgg gtcgcactcc ttctggtcgg ccggcagcgc
     2041 ctcgatgcgc acgccatcgt cgccgtcctg cgggaacttc gggtcggcgt tctgcagcca
     2101 gcacgacacg tcgatcgtct tgtcgcggat cagcaggttg cgcaggatct ggtactcgtg
     2161 gctcgcgccg cccgcgagca gcagcacgcg cgtccgctcg ccgagcacct cgacggcctg
     2221 cgacttctgg tggcgctcgg gcaccgcg
//
LOCUS       DASRDG010000199         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1061373, whole genome shotgun sequence.
ACCESSION   DASRDG010000199 DASRDG010000000
VERSION     DASRDG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061373"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFA35_03280"
     CDS             <1..515
                     /locus_tag="VFA35_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT55222.1"
                     /translation="LAPLFAQVAVDAKLLSAGRHTLVFPTQVEAQVAGASNELLSAIT
                     NLVTNAVRYTPEGGRIELSWRVRADGAGQIAVTDTGPGIAREHLPRLTERFYRVDGSR
                     SRDTGGTGLGLSIVKHVVQRHGGELDIQSEVGQGSTFRLVFPAARVRDGGVPAATGVT
                     TSASEARALP"
     gene            complement(529..2211)
                     /locus_tag="VFA35_03285"
     CDS             complement(529..2211)
                     /locus_tag="VFA35_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55223.1"
                     /translation="MPPVNLPTPALVTQRAAQRLPRVALLLFCAAYVLPGVFGRDPWK
                     SADITAFGYMAGIAQGRTGWLAPTVGGLPADAALLPYWLGAAFIKLLGPWVDPALAAR
                     IPFALLLVLVLVLTWYSCYHLARTEAAQPLPFAFGGEADPIDYARAIADGALLALIAS
                     LGLLQLGHETTPELAQLASVALYVYALAASPFRGLQPKLAVLAALPALAASGAPAIAV
                     VLGIVGALICRASSYPSARRFVAWIAPSTLLSALLASALGAWGWRIGAAAATLPGAWE
                     VLRLLAWFTWPAWPLAIWTVWRWRGHLSARHIAIPLGCTAVAVLACIAMGGSERALML
                     GLPPLAVLAAFALPTLQRSTAAAIDWFSVCLFTIAAIVIWVVYVSMQTGVPAQPAANI
                     ARLSPGYTRSFSAFALALAILGTLAWLWLVKWRTGRNRHPLWKSLVLPASGVALCWLL
                     VMTLLLPPLDHARSYRSLVQRIARQVPTDACIAAPGVARAQVVALEHLGGYRVDAVTT
                     PAATRCEFLLQMETRGSPSAPGPLWKLLARERRNTSEDEVAAIYRRTAAARP"
BASE COUNT          345 a          787 c          871 g          248 t
ORIGIN      
        1 cgctggcgcc gctgttcgcg caggtcgcgg tcgacgcgaa gctgctgtcg gccgggaggc
       61 acacgctggt gttcccgacg caggtcgagg cgcaggtcgc cggcgcatcg aacgagctgc
      121 tcagcgcgat cacgaacctg gtgacgaacg cggtgcgcta cacgcccgag ggcggccgga
      181 tcgaactgag ctggcgcgtg cgcgccgacg gtgccggcca gatcgcggtg accgacaccg
      241 gccccggcat cgcgcgcgag cacctgccgc gcctgaccga gcgcttctac cgggtcgacg
      301 gcagccgctc gcgagacacc ggcggtaccg ggctcgggct gtcgatcgtc aagcatgtgg
      361 tgcagcggca cggcggcgag ctcgacatcc agagcgaggt cgggcagggc tcgacgttcc
      421 ggctcgtgtt cccggcggcg cgggtgcgcg acggcggggt gccggctgcc acgggcgtga
      481 cgacctctgc cagcgaagcg cgcgcgctgc cctgagcgcg cggtgcggct acggccgtgc
      541 ggccgcggtg cggcggtaga tcgcggcgac ctcgtcctcg ctggtattgc ggcgttcgcg
      601 tgcgagcagc ttccagagcg ggccgggtgc gctcggcgac ccgcgcgtct ccatctgcag
      661 caggaactcg cagcgcgtgg ctgccggggt tgtgaccgca tcgacgcggt agccgcccag
      721 gtgctcgagc gccacgacct gcgcgcgcgc cacgcccggc gcggcgatgc aggcgtcggt
      781 cggcacctgt cgtgcgatcc gttgcaccag cgagcggtag ctgcgcgcat ggtcgagcgg
      841 cggcagcagc agggtcatca ccagcagcca gcatagcgcg acaccgctgg ccggcagcac
      901 caggctcttc cacagcgggt gccggttgcg cccggtgcgc cacttcacga gccacagcca
      961 cgcgagcgtg ccgagaatcg ccagcgccag cgcgaacgcc gaaaagctcc gcgtgtagcc
     1021 gggcgagagc cgcgcgatgt tggcggccgg ctgggccggc acgccggtct gcatcgagac
     1081 gtagacgacc cagatcacga tcgccgcgat cgtgaacagg cagaccgaga accagtcgat
     1141 cgccgcggcg gtgctgcgct gcagcgtcgg cagcgcgaat gcggcgagca ccgccagcgg
     1201 cggcaggccg agcatcagtg cgcgctccga gccgcccatc gcgatgcacg cgagcacggc
     1261 aaccgcggtg cagccgagcg ggatcgcgat gtggcgggcc gagaggtggc cacgccagcg
     1321 ccagaccgtc cagatcgcga gcggccacgc cggccaggtg aaccaggcga gcagccgcag
     1381 cacttcccaa gcgcccggca gcgttgccgc tgcggcgccg atgcgccagc cccacgcgcc
     1441 gagcgcggag gccagcagcg ccgacagcag cgtgctcggc gcgatccagg ccacgaaacg
     1501 gcgggcggac gggtagctcg aggcgcggca gatcagggcg ccgacgatgc cgagcacgac
     1561 cgcgatcgcc ggcgcgccac tggcggccag cgcgggcagc gcggcgagca ccgcgagctt
     1621 gggctgcagc ccgcgaaacg gactcgccgc gagcgcgtag acgtacagcg cgacgctcgc
     1681 gagctgcgcc agctccggcg tggtctcgtg gccgagttgc agcagcccga gcgaggcgat
     1741 cagcgcgagc agcgcgccat cggcgatcgc gcgtgcgtag tcgatcgggt cggcctcgcc
     1801 gccgaacgcg aacggcagtg gctgcgcggc ttcggtgcgc gcgaggtggt agcagctgta
     1861 ccaggtcagc accagcacca gcacgaggag caacgcgaac gggatgcgcg cggcgagcgc
     1921 cggatcgacc cacggcccga gcagcttgat gaacgccgcg ccgagccagt agggcagtag
     1981 cgcggcgtcg gcgggcaggc cgccgacggt cggtgcgagc cagccggtgc ggccctgcgc
     2041 gatgccggcc atgtagccga acgcggtgat gtcggcgctc ttccacgggt cgcgcccgaa
     2101 cacgccgggc agcacgtagg cggcgcagaa cagcagcagc gcgacgcgcg gcaggcgctg
     2161 cgccgcgcgc tgggtgacga gggcgggagt tggcagattc acggggggca tcatgccgca
     2221 tgccgaggca tcgctccggc ggcgggctga a
//
LOCUS       DASRDG010000200         5087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_30914, whole genome shotgun sequence.
ACCESSION   DASRDG010000200 DASRDG010000000
VERSION     DASRDG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30914"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFA35_03290"
     CDS             complement(<1..467)
                     /locus_tag="VFA35_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZT55224.1"
                     /translation="MPSTPAHAAPPAVLALIGHTPLVKVTRFDTGPCTLFLKLESQNP
                     GGSIKDRIGVAMIDAAERDGRLQPGGTVVEATAGNTGLGLALVARAKGYRVVLVVPDK
                     MSTEKVLHLKALGAEVHGTRSDVGKGHPDYYQDRAARLAREIPGAFFADQFNNP"
     gene            884..1489
                     /locus_tag="VFA35_03295"
     CDS             884..1489
                     /locus_tag="VFA35_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZT55225.1"
                     /translation="MMIVMRTRMGNARAAAKDATHERIVAVAARAIRRSGYDGTGVAY
                     IMKEAGLTHGAFYAHFASREAMLAEAAGSAFAQAAAALAGIAARVPPDQALASMLEAY
                     LSKEHVAHVATGCPLAALGSETSRQTPDVRRVATRYVKEMIDLVARQLPDWGQPGAHQ
                     RALVTVSTMVGALLLARAVDDSGLSDSLREAALGHLTTSVR"
     gene            1547..2548
                     /locus_tag="VFA35_03300"
     CDS             1547..2548
                     /locus_tag="VFA35_03300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZT55226.1"
                     /translation="MKAFVLERYGKKALLRLADVPKPEPRDDEVLVQVHAAGVNQLDS
                     KIRDGEFKLILPYRLPVILGHDVAGVVVEVGPRVRQFKPGDEVYARVDDFRIGTFAEF
                     VPVKEGSLALKPKGLTMEEAAAVPLVALTAWQALVEKAKLARGQTVFIQAGSGGVGTF
                     AIQLAKHLGATVATTTGTANVAFVKSLGADIVIDYKTQDFADVLRDCDVVLNSQDAKT
                     LDQSLGVLKRGGKLISISGPPDPAFGAAIGAPALVKLVMRWLSWGIRRRAKRRAVDYS
                     FLFMRPDGSQLREITALLDAGAIRPVVDRVFPFESTNEALACVDAGRSRGKVVVKVK"
     gene            2643..3917
                     /locus_tag="VFA35_03305"
     CDS             2643..3917
                     /locus_tag="VFA35_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZT55227.1"
                     /translation="MLAMLATALAAIAETGYVGSFGVGSLAGMALVFPFVMLQMMLSG
                     GAMGGGVSSAVSRALGAGDRDRANALAVHALWIGTGAGALFMALMLALGPWLFSALGG
                     RGEALAQALAYANVAFLGSVFIWLVNTFSSVIRGSGNMSVPSATLLLVALGQVAVSGA
                     LGLGWGPFPRLGMPGVAAGQVLAYGAGAVFLYGYLRSGRAQIQLRLRATPLQWPLARD
                     ILKVGGFACISPLQTVLTILILTRLVAQFGTTALAGYGIGTRLEFLLVPVAFAIGVAS
                     VPLVGMAIGAGRVARARRAAWAASALAAALLGALGAVLALFPDLWTGLFTRDPAVLGA
                     AASYFHWAGPTYGLFGLGLSLYFSSLGAGKVLGPVLAGTLRLGLVAVGGWALALAQTP
                     PWTIFALVALGMAGYGLATALAVRYTAWGVER"
     gene            complement(4068..>5087)
                     /locus_tag="VFA35_03310"
     CDS             complement(4068..>5087)
                     /locus_tag="VFA35_03310"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZT55228.1"
                     /translation="YEGARQKVADFIGAGAVDEIVFVRGTTEGINLVANTWGETHIAA
                     GDEIIVSQLEHHANIVPWQMLATRKGAKLRVIPVDDTGQVILEEFKRLLSDKTKLIAV
                     TQVSNALGTVVPVREIIEIGHRAGATVLVDGAQSVSHMRVDVRALDADFFVFSGHKVF
                     GPTGIGAVYGKRAILDALPPWQGGGNMIQDVTFEKTVYHAPPTRFEAGTGNIADAVGL
                     GAALDYVQRLGIENIGRYEHDLLAYATQQLAPIPGVRLIGTAREKASVLSFVLDGHST
                     DEVGQALNREGIAVRTGHHCAQPILRRFGLEATVRPSLAFYNTCEEVDRFIAVVQRLA
                     GQRRR"
BASE COUNT          676 a         1738 c         1806 g          867 t
ORIGIN      
        1 gggttgttga actggtcggc gaagaatgcc cccgggatct cgcgcgcgag ccgcgcggcg
       61 cggtcctggt agtagtcggg gtggcccttg cccacgtcgc tgcgcgtgcc gtggacctcg
      121 gcgccgagcg ccttcaggtg cagcaccttc tcggtcgaca tcttgtcggg caccaccagc
      181 accacgcgat agcccttggc gcgcgccacc agcgccaggc ccaggccggt gttgccggca
      241 gtcgcctcga ccaccgtgcc gccgggctgc aggcggccgt cgcgctcggc ggcatcgatc
      301 atcgcgacgc cgatgcggtc cttgatcgag ccgccggggt tctgcgattc gagcttcagg
      361 aacagtgtgc acgggccggt gtcgaagcgc gtgaccttga cgagcggcgt gtggccgatc
      421 agcgcaagca ccgcgggcgg ggcggcgtgg gctggggtcg atggcatggc gtctcccttc
      481 tcgtgacgtg gcctcgtgag ccgcgtgtcg agcgatggta gtcgcgctgc acgcccctgc
      541 gcggcgttgt gggcatgcgc tggcttcgat gacctttcac atcgcttgca ggggactcca
      601 gcagtggcct acctgctttc gctggaaggc gagcaacgcg cacgacgttg agatcgtcga
      661 cgatcactga cctggccagg gtcgccaatg gcgtgacaaa gatgagcgtt gcacgcgagg
      721 aactgaaact tgcgcagccc gcatcggccc cacgctcacg gccaattccc ggcgcttgcg
      781 acgggcagct tcgagcaccc cgcattcgct ggcgacgctt gcaagccccc gggaatgacc
      841 tcgcccgcaa gacggtgcgt cgcacggcat gacgagaatc tgtatgatga tcgtcatgcg
      901 aacaagaatg ggcaacgcac gagcggcggc caaggacgcc acacacgaac gcatcgtggc
      961 agtggcggcg cgagcgattc gcaggagtgg ctacgacggc accggcgtcg cctacatcat
     1021 gaaggaggcc ggcctcacgc acggcgcgtt ctacgcccac ttcgcgtcgc gcgaggccat
     1081 gctggcagaa gcggccggca gtgcattcgc gcaggcggcg gcggcgctgg ccggcatcgc
     1141 cgcacgtgtc ccgccggacc aggccttggc gtccatgctc gaggcctacc tgtccaagga
     1201 acacgtcgcg cacgtcgcca ctggctgccc attggccgcg ctgggctccg aaacctcgcg
     1261 ccagacgccc gacgtgcggc gcgtggcgac ccgatacgtc aaggaaatga tcgacctggt
     1321 ggcccgccag ttgccggact ggggccagcc cggcgcgcat caacgcgccc tcgtgacggt
     1381 ctcgacgatg gtgggcgccc tgctgttggc gcgcgcggtc gacgattccg gcctgtcgga
     1441 cagcctgcgc gaagccgcgc tcgggcacct gaccaccagc gtgcgctgaa gccgccatgg
     1501 cccttgcccc gttgggttcg caccttcata tgatgagcat catttcgtga aagcattcgt
     1561 cctcgaacgc tacggcaaga aggccttgct gcggctggcc gacgtgccga aaccggagcc
     1621 gcgcgacgac gaggtcctgg ttcaggttca cgccgccggc gtgaaccagc tcgattccaa
     1681 gatccgcgac ggcgagttca agctcatcct gccgtatcgc ctgccggtca tcctggggca
     1741 cgacgtcgcc ggcgtggtcg tcgaggtcgg cccgcgggtg cggcagttca agccgggcga
     1801 cgaggtctat gcgcgggtcg acgatttccg catcggcacc ttcgccgagt tcgtgcccgt
     1861 caaggaaggc tcgctggcac tcaagccgaa gggcctgacg atggaagaag ccgcggccgt
     1921 cccgctggtg gcgctgacgg cgtggcaggc gctggtcgag aaggccaaac tcgcgagggg
     1981 gcaaacagtc ttcatccagg cgggctccgg cggcgtggga accttcgcca tccaactggc
     2041 caagcacctg ggcgccaccg ttgccacgac gaccggcacg gccaatgtcg cgttcgtgaa
     2101 gagtctgggc gccgacatcg tcatcgacta caagacgcag gacttcgccg acgtcttgcg
     2161 cgactgcgac gtggtgctga acagccagga cgccaagacg ctcgaccagt cgctcggtgt
     2221 gctcaagcgc ggcggcaagc tcatctccat ttccgggccg cccgatcccg cgttcggcgc
     2281 ggccatcggt gcacccgcgc tcgtgaagct ggtgatgcgg tggctgagct ggggcatccg
     2341 gcgccgggcg aagcgccgcg cggtcgacta ctcgttcctc ttcatgcggc ccgacggcag
     2401 ccagttgcgc gagatcaccg ccctgctcga tgccggcgcg atccggcccg tcgtggaccg
     2461 cgtcttcccg ttcgagtcga cgaacgaagc gctggcctgc gtggacgccg ggcgttcccg
     2521 gggcaaggtc gtcgtcaagg tcaagtgagc ccgttttgat gccatccacc gccgccctgt
     2581 cggacagccc gctgctgcac gccccgctgc tgccgacgct gctgcgcctt gccttgccca
     2641 acatgctggc catgctggcc accgcgctgg cggcgatcgc ggagaccggc tacgtgggca
     2701 gtttcggcgt cggctcgctg gccgggatgg cgctggtctt tccgttcgtg atgctgcaga
     2761 tgatgctgtc cggtggcgcg atgggcggcg gcgtgtcgtc ggcggtgagc cgtgcgctcg
     2821 gcgccggcga tcgggaccgt gccaacgcgc tggcggtgca tgcgttgtgg atcggcaccg
     2881 gcgcgggcgc gctgttcatg gccctgatgc tggccctcgg cccgtggctg ttttcggcgc
     2941 tcggcggccg gggcgaggcg ctggcccagg cgctggccta cgcgaacgtc gcgtttctcg
     3001 ggtcggtctt catctggctg gtcaatacct tctcgtcggt gatccggggg tcgggcaaca
     3061 tgtcggtgcc gtcggccacc ttgctgctgg tggcgttggg gcaggtggcg gtcagcgggg
     3121 cgctgggcct gggttggggg ccgttcccgc gcctgggcat gcccggcgtg gccgcggggc
     3181 aggtgctcgc ctatggcgcg ggcgcggtgt tcctgtacgg gtacctgcgc tccgggcggg
     3241 cgcagattca gttgcgcctg cgtgccacgc cgctgcaatg gccactggcg cgcgacatcc
     3301 tgaaggtcgg cggctttgcc tgcatctcgc cattgcagac ggtgctgacc atcctgatcc
     3361 tgacgcgcct ggtcgcgcag ttcggcacca ccgccctcgc gggctacggc atcggcacgc
     3421 gcttggagtt cctgctggtg ccggtggcct tcgcgatcgg ggtcgcgtcg gttccgctgg
     3481 tcggcatggc catcggcgcc gggcgggtcg cgcgcgcccg tcgggcggcc tgggccgctt
     3541 cggcgctggc cgccgcgctg ctgggcgcgc tgggcgccgt gctggcactg ttccccgatc
     3601 tctggacggg cctgttcacg cgggatccgg cagtcctggg cgccgccgcc agctatttcc
     3661 actgggccgg gccgacctac gggctgttcg gtctcgggct gtccctgtac ttctcgtcgc
     3721 tcggcgccgg caaggtgctc ggcccggtgc tggcgggcac gctgcggctg ggcttggtgg
     3781 cggtcggcgg ctgggcgctg gcgctggcgc aaacgccgcc gtggaccatc ttcgcgctgg
     3841 tcgcgctggg catggccggg tacggattgg ccaccgcact ggccgtgcgc tacaccgcct
     3901 ggggcgtcga gcgctgacgc tgttccaggc ggaccaactt ggccggcttt gcgccggcca
     3961 aggccgcgcg gtgcatcccg gtgggctccg cggcacgcga tcctgactgg attgcttctg
     4021 ggtcgccagc agacttcaga cgcctgatcc gctggcctgc acctggctca gcgccgccgc
     4081 tgccccgcca gccgctgcac caccgcaatg aaccgatcca cctcttcgca ggtgttgtag
     4141 aacgccagcg acggccgcac cgtcgcctcc aacccgaagc gccgcaggat cggctgcgcg
     4201 cagtggtggc cggtgcgcac cgcgatgccc tcgcggttca gcgcctggcc gacttcgtcg
     4261 gtcgagtggc cgtcgagcac gaacgacagc acgctggcct tctcgcgcgc ggtgccgatc
     4321 agccgcacgc cgggtatcgg cgccagctgt tgcgtcgcgt acgcgagcag gtcgtgctcg
     4381 tagcggccga tgttctcgat gccgaggcgc tgcacgtagt cgagcgccgc gcccaggccc
     4441 accgcgtcgg cgatgttgcc ggtgccggcc tcgaagcgcg tcggcggcgc gtggtagacg
     4501 gtcttctcga aggtcacgtc ctggatcatg ttgccgccgc cctgccacgg cggcagcgcg
     4561 tcgagaatcg cgcgcttgcc gtagaccgcg ccgatgccgg tcgggccgaa gaccttgtgg
     4621 ccgctgaaga cgaagaagtc ggcgtcgagc gcgcgcacgt cgacgcgcat gtgcgagacc
     4681 gattgcgcgc cgtcgaccag cacggtcgcg ccggcgcggt ggccgatctc gatgatctcg
     4741 cgcaccggca cgacggtgcc gagcgcgttc gagacctgcg tgaccgcaat cagcttcgtc
     4801 ttgtcggaca aaaggcgctt gaactcttcg aggatcacct gccccgtgtc gtcgaccggg
     4861 atcacgcgca gcttcgcgcc cttgcgggtg gccagcatct gccacggcac gatgttggcg
     4921 tggtgctcga gctgcgagac gatgatctcg tcgccggccg caatgtgcgt ttcgccccag
     4981 gtgttcgcga ccaggttgat gccttcggtc gtgccgcgca cgaacacgat ctcgtcgacc
     5041 gcgccggcgc cgatgaagtc ggccaccttc tggcgcgcgc cctcgta
//
LOCUS       DASRDG010000201         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1061654, whole genome shotgun sequence.
ACCESSION   DASRDG010000201 DASRDG010000000
VERSION     DASRDG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061654"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VFA35_03315"
     CDS             complement(<1..1126)
                     /locus_tag="VFA35_03315"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HZT55229.1"
                     /translation="MDELNSVWQWLARTMGPYAPRLIGALTIAVIAWIVARLLRSGLL
                     RLAGARQLDERLRSPGLGARLADVAYWLVWLLALPALLETLQMQGLLAPVQAMMARLL
                     GFLPSLFGAAVVLGVGLLVAQIVRQVVSGLLRAAGSERLAARLGLSAAVGEEGLAGLA
                     GLIVFVLVLLPVVAGALQPLGLDAVTSPVSRLLDSVIAVIPKLVAAAIIIVIAALVGR
                     ALSSLVTALLAGIGFNALPGKLGLPGSVRAGGRSAAELGGALAMAAVMLIAVTQACEV
                     LGFAALTEMVTTLGVVLARLLVAVLLFGVGLWLATLASNTIRSSAVANAPVLAGIARV
                     AILFFTAALALRQAGLPAEIVTIAFASVIGALAVGVAIAIG"
     gene            complement(1272..2318)
                     /gene="add"
                     /locus_tag="VFA35_03320"
     CDS             complement(1272..2318)
                     /gene="add"
                     /locus_tag="VFA35_03320"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZT55230.1"
                     /translation="MTSPPAASPNFHTLPKALLHEHIDGGLRPTTLLELAHARGLALP
                     TDDPAALADWMHANANSGSLERYLRGFALTVGAMATPEACERVAFEAAEDARRDGCVL
                     AEFRMAPLLLEPFGLRGEAVVEALAAGLARSALPCGLIVCAMRTDAPEETLRAADLAA
                     RYAGKSAGRGVIGFDLAGAERGFPPGAHAAAFARARGAGLGVTCHAGESDAGSRVLEA
                     AALGATRIGHGVHIMSGRSADETARWVDQARALGLHFEVCPSSNIHTGAFASLAEHPI
                     RKMVEAGLSVSCSTDNRLMSGVTLSDELAAVHAAPGLSTAQLAQTMRSAAAASFLPAD
                     VRAAALHAMQAWPD"
     gene            2520..3497
                     /locus_tag="VFA35_03325"
     CDS             2520..3497
                     /locus_tag="VFA35_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT55231.1"
                     /translation="MKISSLQLGLLALAASLSLAAQAEPAVVYDMGGKFDKSFNEAAY
                     RGIERWKKETGKPYLEFEIANETQREQALRRMAERGASPIIGIGFGQAAAIEKIAKEF
                     PKLQFALIDMVVQLPNVESVVFKEQEGSFLVGVMATAASKTGKVGFIGGMDIPLIRKF
                     QCGYEQGAKYANPKTEVFSNMTGTTGAAWADPARGSELAKAQFGKGADVVFAAAGGTG
                     MGVYQAAKDAGKLAIGVDSNQNHIQPGTMLTSMLKRVDVAVYEAAKGWKPGVTVLGLK
                     EGGIDYAMDEYNAKLVSADMKKKVEAAKADIIAGKIKVADYMADNACKL"
     gene            3529..>3988
                     /locus_tag="VFA35_03330"
     CDS             3529..>3988
                     /locus_tag="VFA35_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZT55232.1"
                     /translation="MSTIKHVAARAGVSFTTVSHVLNGTRRVSDSARERVERAIAEMG
                     YSPSAVARALKMSETGIIGVLVPNITNPFFAELTRGIEDCCRRTDYSVFLCNSDDDPE
                     RQARYLQTLLERRVDGLLLAAAAGEAAVLAQRLSTARVPTVVVDRAIPGLA"
BASE COUNT          658 a         1443 c         1400 g          487 t
ORIGIN      
        1 cgccgatcgc gatcgcgaca ccgaccgcga gcgcgccgat caccgacgcg aacgcgatcg
       61 tcacgatctc ggccggcagc ccggcctggc gcagcgccag cgccgcggtg aagaacagga
      121 tcgccacgcg cgcgatgccg gccaggaccg gcgcgttggc caccgcgctc gaccggatcg
      181 tgttcgacgc gagcgtcgcc agccacaagc cgacgccgaa cagcagcacc gccaccagca
      241 gcctggcgag cacgacgccg agcgtcgtga ccatctcggt cagcgcggcg aagccgagca
      301 cctcgcaggc ctgcgtgacg gcgatcagca tcaccgccgc catggccagc gcgccaccca
      361 gttcggccgc gctgcgaccg ccggcacgca cgctgcccgg gaggccgagc ttgccgggca
      421 gcgcgttgaa gccgatgccg gcgagcagcg cggtcacgag gctcgacagc gcgcgcccga
      481 ccagcgcggc gatcacgatg atgatggccg ccgcgaccag cttcgggatc accgcgatca
      541 cgctgtccag cagccggctc accgggctcg tgaccgcatc cagcccgagc ggctgcaagg
      601 cgcccgccac gaccggcagc agcaccagca cgaacacgat caagccggcc agcccggcca
      661 gcccctcctc gccgaccgcc gccgacaggc ccagccgggc cgcgaggcgt tcggagccgg
      721 cggcgcgcag caggccgctg acgacctgcc ggacgatctg cgcgaccagc aggccgacgc
      781 cgagaacgac cgcggcgccg aacagacttg gcaggaagcc cagcaggcgc gccatcatgg
      841 cctgcaccgg cgccagcagg ccctgcatct gcagcgtttc gagcagcgcc ggcagcgcca
      901 gcagccagac cagccagtag gcgacatcgg ccaggcgcgc gccgaggccg gggctgcgca
      961 ggcgctcgtc cagctgccgg gcgccggcca ggcgcagcag gcccgagcgc agcagccgcg
     1021 cgacgatcca ggcgatcacg gcgatcgtca gcgccccgat caggcgtggc gcatacgggc
     1081 ccatcgtgcg cgccagccat tgccagacgc tgttcagttc atccatcgca gcttcctcag
     1141 gggtatgggg tcgggtgatg acaggggtgc gtggcgacac gccgcacgag cctacgcggg
     1201 atcatagggt gacgcacccg cccgcagcat gggtgcgacc ccgtctcacg tctggccccg
     1261 ccgctgctca ctcaatcagg ccaggcctgc atcgcgtgca gcgcggcggc gcgcacgtcg
     1321 gcgggcagga acgacgccgc cgccgcgctg cgcatcgtct gcgcgagctg cgcggtgctc
     1381 aagcccggtg cggcgtggac cgccgccaac tcgtcgctca gcgtcacgcc cgacatcagc
     1441 cggttgtcgg tcgagcacga caccgacagg ccggcctcga ccatcttgcg gatcgggtgc
     1501 tcggccagcg acgcgaacgc gccggtgtgg atattgctgc tcgggcagac ctcgaagtgc
     1561 aggccgagcg cgcgcgcctg gtcgacccag cgcgcggtct cgtcggcgct gcggcccgac
     1621 atgatgtgca cgccgtggcc gatgcgcgtt gcgccgagcg cggccgcttc gagcacgcgc
     1681 gagcccgcgt cggattcgcc ggcatggcag gtcaccccga gcccggcgcc gcgagcgcgc
     1741 gcgaatgcgg cggcgtgcgc gccgggcggg aagccgcgct cggcgccggc caggtcgaag
     1801 ccgatcacgc cgcggccggc gcttttgccg gcataccggg ccgcgaggtc ggcggcgcgc
     1861 agcgtttctt cgggcgcatc ggtacgcatc gcgcagacga tcagcccgca tgggagcgcg
     1921 ctgcgcgcca ggcccgccgc cagcgcctcg acgacggcct cgccgcgcag cccgaacggc
     1981 tcgagcagca acggcgccat gcggaactcg gcgagcacgc agccgtcgcg ccgcgcgtct
     2041 tcggcggcct cgaacgcgac gcgctcgcag gcctcgggcg tggccatcgc gcccaccgtc
     2101 agcgcgaagc cgcgcaggta gcgttccagg ctgccggagt tggcgttcgc gtgcatccag
     2161 tcggccagcg ccgcggggtc gtcggtcggc agcgcgaggc cgcgcgcatg cgcgagttcg
     2221 agcagcgtcg tcgggcgcag gccgccgtcg atgtgctcgt gcagcagcgc cttcggcagg
     2281 gtgtggaaat tcggggaggc ggcaggcggc gaggtcacgg gcaaaggtct ttcgagcggt
     2341 gatcgggctg ggtcagcagg attcgcgcca ctgcgggggc aggccgccac gggaaaccac
     2401 ccgtcgaacg ggcccggttt ctgcttaaag tgcaaaactg gcaaacgttt gcgcccaatt
     2461 gctgcgcgca aaatcgcgcc atcatgcacc gccccgaacc ttgccacctg agacagacca
     2521 tgaaaatctc ctccctccag ctcggattgc ttgccctcgc cgcctcgctc agcctcgcgg
     2581 cgcaggccga gcccgccgtc gtctacgaca tgggcggcaa gttcgacaag tcgttcaacg
     2641 aggccgccta ccgcggcatc gagcgctgga agaaggagac cggcaagcct tacctcgagt
     2701 tcgagatcgc caacgagacc cagcgcgagc aggccctgcg ccgcatggcc gagcgcggcg
     2761 cgagcccgat catcggcatc ggcttcggcc aggccgcggc gatcgagaag atcgcgaagg
     2821 agtttccgaa gctgcagttc gcgctgatcg acatggtggt gcaactaccc aacgtggagt
     2881 cggtggtgtt caaggagcag gaaggcagct tcctggtcgg cgtgatggcg acggccgcga
     2941 gcaagaccgg caaggtcggc ttcatcggcg gcatggacat cccgttgatc cgcaagttcc
     3001 agtgcggcta cgagcagggc gcgaagtacg cgaacccgaa gaccgaggtg ttctcgaaca
     3061 tgaccggcac caccggcgcc gcctgggccg acccggcgcg cggcagcgag ctcgcgaagg
     3121 cgcagttcgg caagggcgcc gacgtcgtgt tcgcggccgc cggcggcacc ggcatgggcg
     3181 tgtaccaggc cgccaaggac gccggcaagc tcgcgatcgg cgtcgacagc aaccagaacc
     3241 acatccagcc tggcaccatg ctgacctcga tgctcaagcg cgtggatgtc gcggtgtacg
     3301 aggccgccaa gggctggaag ccgggcgtca ccgtgctcgg cctgaaggaa ggcggcatcg
     3361 actacgcgat ggacgagtac aacgccaagc tcgtcagcgc cgacatgaag aagaaggtcg
     3421 aggccgccaa ggccgacatc atcgccggca agatcaaggt ggccgactac atggccgaca
     3481 acgcctgcaa gctctgagtc ggcgttcgga ccagcacagg attccggctt gagcaccatc
     3541 aagcacgtcg cggcgcgcgc gggcgtgtcg ttcacgaccg tctcgcacgt gctcaacggc
     3601 acgcgtcggg tcagcgactc ggcgcgcgag cgcgtcgagc gtgcgatcgc cgagatgggc
     3661 tattcgccca gcgcggtggc gcgggcgctg aagatgagcg agaccggcat catcggcgtg
     3721 ctcgtgccca acatcacgaa cccgttcttc gccgagctca cgcgcggcat cgaagactgc
     3781 tgtcggcgca ccgactactc ggtgttcctg tgcaactccg acgacgaccc cgagcgtcag
     3841 gcgcgctacc tgcagaccct gctcgagcgc cgcgtcgacg gcctgctgct cgcggccgcc
     3901 gcgggcgagg ccgcggtgct cgcgcagcgg ctctcgaccg cgcgcgtgcc gaccgtcgtc
     3961 gtcgaccgcg cgatccccgg cctcgcgg
//
LOCUS       DASRDG010000202         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1541693, whole genome shotgun sequence.
ACCESSION   DASRDG010000202 DASRDG010000000
VERSION     DASRDG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541693"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFA35_03335"
     CDS             complement(<1..265)
                     /locus_tag="VFA35_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZT55233.1"
                     /translation="MMEDKKGPHGIPRVDLPDDVRLDTLAVREGLPRSAWGENSEALF
                     LTSSFVQPDAATAAARFANEEEAFIYSRFTNPTVTMMERRLAAL"
     gene            complement(262..1785)
                     /gene="purF"
                     /locus_tag="VFA35_03340"
     CDS             complement(262..1785)
                     /gene="purF"
                     /locus_tag="VFA35_03340"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZT55234.1"
                     /translation="MCGIVGVISRGAVNQLIYDALLLLQHRGQDAAGIVTMQGTKCFM
                     HKARGMVRDVFRTRNMRGLPGTVGLGQVRYPTAGNAYSEEEAQPFYVNAPFGIVLVHN
                     GNLTNAAALKQELVDIDRRHINTTSDTEVLINVVAHELALATRDQPLTPDLVFAAVRA
                     VHKRIKGSYAVVALIAGHGLLAFRDPFGIRPLCVGEGTGPEGREVMVASESVAIEGTG
                     HQVIRDVAPGEALFIDLEGTIHARQCADNPSLNPCMFEYVYLARPDSVMDGISVYQAR
                     LNMGETLAQRLISTIPPNEIDVVIPIPESSRPSAMQLAQRIGKPYREGFVKNRYVGRT
                     FIMPGQGARKKSVRQKLNAIGVEFKGRNVLLVDDSIVRGNTSKEIVQMAREAGARKVY
                     MASAAPPVRYPNVYGIDMPTSEELIAHNRSIEEIREFIGADALIYQDVNAMKRVVRAL
                     NPALQGFEASCFDGVYITGDVGGDYFDAMAAQRRSQGDEDEGEERSRLALQSATEGP"
     gene            complement(1823..2326)
                     /locus_tag="VFA35_03345"
     CDS             complement(1823..2326)
                     /locus_tag="VFA35_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZT55235.1"
                     /translation="MTIDLGWVDIGLAVFLLLSVLIGAARGFVFELLSLAGWFAAWFA
                     GWWLTPTFAGHIRIGAPGSSLNYGVTFACVFLVALVVWSLGARLVRALVRATPLSAVD
                     RLLGAGFGFVRGLVVLLVAATVIGISPWGHSIAWQRSQGVAWLNTVLHELRPLFINDT
                     PPQNPTA"
     gene            complement(2336..2986)
                     /locus_tag="VFA35_03350"
     CDS             complement(2336..2986)
                     /locus_tag="VFA35_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZT55236.1"
                     /translation="MLVVIGIIGFPLVFETQPRPIPVDIPIEVPRKEGAPALVMPAAR
                     PSPARPSASAAPAVPNDVITESREEAGREVAVAAAAPAPAAASKPAPKPAAKPPVAAA
                     VEAKPAAKTETKPEAKPSGGDSARARALLEGKPAAAADSGRFIVQVGAFADAGAARET
                     RLKAEKLGLKTYTQVASTASGNRIRVRVGPFGSRDEADKAMAKAKGAGLNAVVLTL"
     gene            complement(3228..4535)
                     /gene="folC"
                     /locus_tag="VFA35_03355"
     CDS             complement(3228..4535)
                     /gene="folC"
                     /locus_tag="VFA35_03355"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HZT55237.1"
                     /translation="MTTELPQTLADWLTRCERLHPKEIDMTLERTRRVKEQLNLHFSM
                     PMIAVAGTNGKGSTCAMLEAIALQAGYRVGLYSKPHLVHFEERCRINGRMVDGEALLP
                     HFLAVERARGDISLTYFEFTLLAIAGLLAETPLDLVIFEVGMGGRLDAVNAFDADCAV
                     ITSIDIDHAEFLGADRETIAIEKAGIMRAGRPVVVSDPVPPQSLADEAARIGADLWQF
                     GRDFNYAGDKQQWNWSGRARRFNALAYPALRGANQLLNASGVLAAFEALRQRLPITAQ
                     AVRQGFATVELPGRFQIVAGQPTLVLDVAHNPHAVAALAQNLDQMGFFPRTHAVFGAM
                     ADKDLVAILTRMAPLVDHWHFTDLPVARATPAAELAALHAGLGLKGPGPVTVECHGSP
                     GQALDAALGRADPADRIVVFGSFLTVGGVLTDGTPRLAGKHAA"
     gene            complement(4636..4734)
                     /locus_tag="VFA35_03360"
     CDS             complement(4636..4734)
                     /locus_tag="VFA35_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZT55238.1"
                     /translation="MITAVPEPSTYALMLAGLAGIGFVARRRTQQR"
BASE COUNT          743 a         1663 c         1644 g          760 t
ORIGIN      
        1 cgagcgcggc caggcggcgc tccatcatcg tcaccgtcgg gttggtgaag cgcgaataga
       61 tgaacgcctc ttcctcgttg gcgaagcgcg ccgccgcggt cgcggcatcg ggctgcacga
      121 agctgctggt caagaacagc gcctcggagt tctcgcccca ggcgctgcgc ggcaagccct
      181 cgcgcaccgc cagcgtgtcc agccgcacgt cgtcgggcag gtcgacgcgc ggaatgccgt
      241 ggggcccctt cttgtcttcc atcatgggcc ctcggtagcg ctttgcaggg cgagccgcga
      301 acgctcctcg ccctcgtcct cgtcgccttg cgagcggcgc tgcgcggcca tcgcgtcgaa
      361 gtagtcgccg cccacgtcgc cggtgatgta gacgccgtcg aagcacgagg cctcgaagcc
      421 ctgcaacgcc gggttcagcg cgcggacgac gcgcttcatc gcgttcacgt cctggtagat
      481 cagcgcatcg gcgccgatga actcgcggat ctcctcgatg ctgcggttgt gcgcgatcag
      541 ctcttcgctg gtcggcatgt cgatgccgta tacgttcggg tagcgcaccg gcggcgcggc
      601 cgaggccatg tagaccttgc gggcgccggc ttcgcgcgcc atctgcacga tctccttgct
      661 ggtgttgccg cgcacgatcg agtcgtcgac cagcagcacg ttgcggccct tgaactcgac
      721 gccgatcgcg ttgagctttt gccgcaccga cttcttgcgt gcgccctgcc ccggcatgat
      781 gaaggttcgg ccgacgtagc ggttcttcac gaacccctcg cggtacggct tgccgatgcg
      841 ctgcgcgagc tgcatcgccg aggggcgact cgattcgggg atcgggatca cgacgtcgat
      901 ctcgttcggc gggatcgtcg agatcagccg ctgcgcgagc gtctcgccca tgttcaggcg
      961 cgcctggtag accgagatgc cgtccatgac cgagtccggc cgcgccaggt acacgtactc
     1021 gaacatgcag gggttcaggc tcgggttgtc ggcgcactgg cgcgcgtgaa tcgtgccttc
     1081 gaggtcgatg aacagcgcct cgccaggggc cacatcacgg atcacctggt gccccgtgcc
     1141 ttcgatcgcg accgattcgc tcgcgaccat cacctcgcgc ccttccgggc ccgtgccttc
     1201 gccgacgcac agcggccgga tgccgaacgg gtcgcggaac gccagcagcc cgtggcccgc
     1261 gatcagtgcg acgaccgcat acgagccctt gatgcgcttg tgcaccgcgc gcaccgcggc
     1321 gaacacgaga tcgggcgtca gcggctgatc gcgcgtggcc agcgcgagct cgtgcgcgac
     1381 gacgttgatc agcacctcgg tgtcgctggt cgtgttgatg tggcgccggt cgatgtcgac
     1441 cagctcctgc ttcagcgccg ccgcgttggt caggttgccg ttgtgcacga gcacgatgcc
     1501 gaacggcgcg ttgacgtaga acggctgcgc ctcttcttcg ctgtaggcat tgccggccgt
     1561 cggatagcgc acctggccca ggcccacggt gcccggcagc ccgcgcatgt tgcgggtgcg
     1621 gaacacgtcg cgcaccatgc cgcgcgcctt gtgcatgaag cacttggtgc cctgcatcgt
     1681 gacgataccg gcggcgtcct gcccgcggtg ctgcagcagc agcagcgcgt cgtagatcag
     1741 ctggttgact gcgcccctgg agatgacgcc gacgatgccg cacatgattc gttgccctag
     1801 gttcaacccg cgatgcgtgg ggtcacgctg tcgggttttg aggaggagtg tcgttgatga
     1861 agagcggtcg cagctcgtgc agcaccgtat tcaaccaggc cacgccctgc gagcgttgcc
     1921 aggcgatcga atggccccac ggcgaaatgc cgatcaccgt ggcggcaacg agcagcacga
     1981 ccagcccgcg cacgaacccg aagccggcac cgagcagccg atcgaccgcg ctcagcggcg
     2041 tcgcccggac cagcgcccgc accagccgcg cacccaggct ccagacgacg agcgcgacca
     2101 ggaacacgca cgcgaaggtc acgccgtagt tcaagctcga acccggcgcg ccgatgcgga
     2161 tatggcccgc gaacgtcggc gtcagccacc agccggcgaa ccaggccgca aaccagccgg
     2221 ccagcgacag cagttcgaac acgaagccac gcgccgcgcc gatcagcacc gacagcagca
     2281 ggaacacagc gagcccgatg tccacccagc cgaggtcgat ggtcatgcgg ccgcctcaca
     2341 gcgtgagcac gaccgcgttc agcccggcac ccttggcctt ggccatcgcc ttgtcggctt
     2401 cgtcgcggct gccgaacggg cccacgcgca cgcgaatgcg gttgccgctc gcagtgctgg
     2461 ccacctgggt gtaggtcttc aggccgagct tctcggcctt cagccgggtc tcgcgcgcgg
     2521 cgcctgcgtc ggcaaacgcg ccgacctgca cgatgaagcg gccggaatcc gcggccgccg
     2581 ctggtttgcc ttcgagcagc gcgcgggcac gtgcactgtc gccgccggac ggcttggctt
     2641 cgggtttggt ttccgtcttg gccgcgggct tcgcctcgac ggcagccgcc acgggcggct
     2701 tcgctgccgg cttgggcgcg ggcttcgagg ccgccgccgg ggccggcgca gcggcggcca
     2761 cggccacctc gcgacccgct tcctcgcgcg actcggtgat cacgtcgttc ggcaccgcag
     2821 gcgccgccga agcggatggg cgggccggcg atgggcgcgc cgccggcatc acgagcgccg
     2881 gcgcgccttc cttgcgcggc acctcgatcg ggatgtcgac cgggatcggc cgcggctggg
     2941 tttcgaacac cagcggaaaa ccgatgatcc cgataaccac cagcactgcc gcaccgatca
     3001 gccgttggcg agcgcgtgtc cgagcgcgtt gcaccgcatc ggccggttcg ctcgccgccg
     3061 ggttgggagc ggcatcagcg cgcttgaaaa tggacagcag acccatgcgg tgcgagttcc
     3121 ggtgatgcga agtggtgcgt ggcttgtgag gactctcgcc cgcggccaca agccgcaggc
     3181 cggttgcgcc gggcccggcc ggcgcgcctg gcgcctgcga agggcgttca ggccgcgtgt
     3241 ttgccagcca aacggggggt gccgtccgtc agcacgccac cgaccgtgag gaacgatccg
     3301 aagacgacga ttctatcagc ggggtcggcg cggccgagcg ccgcatcgag cgcctgcccc
     3361 gggctgccat ggcattcgac cgtgaccgga ccggggcctt tcaagcccaa gccggcatgc
     3421 agcgccgcga gctcggcggc gggcgtggcc cgcgcaacgg gcaggtcggt gaagtgccag
     3481 tgatcgacga gcggcgccat gcgggtgagg atcgccacga ggtccttgtc ggccattgcg
     3541 ccgaatacgg cgtgggtgcg cgggaagaaa cccatctggt ccaggttctg cgccagcgcc
     3601 gccaccgcgt gcgggttgtg cgcgacatcg agcaccagcg tcggctggcc ggcgacgatc
     3661 tggaagcggc ccggcaactc gaccgtcgcg aagccctggc ggaccgcctg cgcggtgatc
     3721 ggcaagcgct ggcgcagcgc ctcgaacgca gccagcacgc ccgacgcgtt cagcaactga
     3781 ttcgcgcccc gcagtgccgg gtacgccagc gcattgaagc ggcgcgcacg gcccgaccag
     3841 ttccactgtt gcttgtcgcc cgcgtagttg aaatcgcggc cgaattgcca caggtcggcg
     3901 ccgatgcgcg ctgcctcgtc ggcgaggctt tgcggcggca ccgggtcgct gacgaccacc
     3961 ggtctgccgg cgcgcatgat gccggccttc tcgatcgcga tcgtctcgcg gtcggcgccg
     4021 aggaactcgg cgtgatcgat gtcgatgctg gtgatgacgg cgcagtcggc atcgaaggcg
     4081 ttcacggcat cgagccggcc gcccatgccg acttcgaaga tcacgagatc caacggcgtc
     4141 tcggccagca gccccgcgat cgccaacagc gtgaactcga aataggtcag gctgatgtcg
     4201 ccgcgcgctc gctcgacagc caggaagtgc ggcagcagcg cttcaccgtc gaccatccgg
     4261 ccgttgatgc ggcagcgctc ctcgaagtgc acgaggtgcg gcttcgagta cagccctacg
     4321 cggtagcccg cctgcaaggc aatcgcctcg agcatcgcgc aggtcgagcc tttgccgttc
     4381 gtgcccgcga ccgcgatcat cggcatgctg aagtgcaggt tcagttgctc cttgacgcgg
     4441 cgcgtgcgct ccagcgtcat gtcgatctct ttggggtgca atcgctcgca gcgcgtcagc
     4501 cagtccgcaa gggtttgggg cagttcggtc gtcatggcgc gatgttacgt ggccgaaaac
     4561 ggccaacgcg gccgaagccg cgctttagaa cgcgaaaagg cggccgaagc cgcctttgtc
     4621 gagatcgaga tgcgatcagc gctgctgggt gcgccggcga gccacgaaac cgatgccagc
     4681 caggccggcg agcatcagcg cgtaggtcga aggctccgga accgcggtga tcaccagcaa
     4741 atcctggtgc ccttcgctgt gcagttgatc gaccttatac aggctgggtg cgccggcgaa
     4801 ccccgcccaa
//
LOCUS       DASRDG010000203         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1062655, whole genome shotgun sequence.
ACCESSION   DASRDG010000203 DASRDG010000000
VERSION     DASRDG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062655"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VFA35_03365"
     CDS             <1..869
                     /locus_tag="VFA35_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZT55239.1"
                     /translation="HMLDASGLVTFYKTDKEGQDRLENLDELVNAAEAFVTQEGFGKD
                     AVALPVDEHGVAIEQAPDAETGEIMSPLAAFLTHASLEAGDNQAQAGQDAIQLMTVHS
                     AKGLEFDCVFITGLEEGLFPHENSAADPDGLEEERRLMYVAITRARKRLYLSFAQTRM
                     LHGQTRYNVKSRFLDELPEAALKWITPRNQGFGSGFQRDYQAAWARGSGLNSIVGAGN
                     VSGVPSAPPLVPKAPGHGLRSGQSVFHTKFGEGVIVTLEGSGADARAQVNFGRHGMKW
                     LQVSLAKLTPVD"
     gene            882..1508
                     /locus_tag="VFA35_03370"
     CDS             882..1508
                     /locus_tag="VFA35_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55240.1"
                     /translation="MNTMTRSRTVLAAVAVAAAALAGCATTQVDAQWRSVELPPSYLR
                     GASVLVSCETGEEVIKRICEDQLGADLRARGVRVLVAARGALPAVAPAGPTDAQYLPL
                     ARENGAKAVLSVSVGPSSQSVSPGISIGIGGFGFGRHSAGGIGVSAPIGGGQVSQGFA
                     ANGRVTDVASGRLMWTARASSPPSSDVNAQIAELSTSVLDAAATAGLF"
     gene            complement(1512..>2425)
                     /locus_tag="VFA35_03375"
     CDS             complement(1512..>2425)
                     /locus_tag="VFA35_03375"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HZT55241.1"
                     /translation="SDPALQAAHAAVPWLLVWDDHEVGNDYAGLQGQDLEPDFKAQRA
                     AAYRAYWEHMPFPKSARPLDADMRITGRLDWGLLARIHLLDDRQYRDPQVCPKPAKGG
                     GSNTVALKACPEMLDPKRTLLGAAQEQWLADGWDTHRPWNLLAQQTLMARLTSTAAAN
                     GGGTYWTDGWDGYPAARQRLLGVVAQRKVPGVVVLGGDVHSNFVADLKADFDDAEAPV
                     IASEFCGTSITSLSMAQSRLDAMRPFNPHLRYARSDQRGYVRFTLDAKQLQAQLRVVD
                     DALDPGSGITTAARFVVEASRPGAIAA"
BASE COUNT          350 a          836 c          883 g          356 t
ORIGIN      
        1 agcacatgct cgacgcctcg ggcctggtca cgttctacaa gaccgacaag gaaggccagg
       61 accgcctcga gaacctcgac gaactcgtca acgcggccga ggccttcgtg acgcaggagg
      121 gcttcggcaa ggacgcggtc gcgctgccgg tcgacgagca tggtgtcgcc atcgagcagg
      181 cgcccgatgc cgagaccggc gagatcatgt cgccgctggc cgcgttcctg acccacgcgt
      241 cgctcgaagc cggcgacaac caggcgcagg ccgggcagga cgcgatccag ctgatgaccg
      301 tgcactcggc caagggcctg gagttcgact gcgtcttcat caccggcctc gaagaaggcc
      361 tgttcccgca cgaaaactcg gccgccgacc ccgacgggct cgaagaagag cgccgcctga
      421 tgtacgtcgc gatcacgcgc gcgcgcaagc gcctgtacct cagcttcgcg cagacccgca
      481 tgctgcacgg ccagacgcgc tacaacgtca agagccgctt tctcgacgag ctgccggagg
      541 ccgcgctgaa gtggatcacg ccgcgcaacc agggcttcgg ctcgggcttc cagcgcgact
      601 accaggcggc gtgggcgcgt ggctcggggt tgaattcgat cgtcggcgcg ggcaacgtca
      661 gcggcgtccc gagtgctccg ccgctggtgc cgaaggcgcc gggccacggt ctgcgctcgg
      721 ggcagagcgt gttccacacc aagttcggcg agggcgtgat cgtcacgctc gaaggcagcg
      781 gcgccgatgc gcgcgcgcag gtcaatttcg gccggcatgg catgaagtgg ctgcaggtct
      841 cgcttgcgaa actcacgccg gtggactgaa ggagagaccc gatgaacacg atgacccgct
      901 ccagaaccgt tcttgccgcc gtcgcggtcg cggccgccgc gctcgccggc tgcgccacga
      961 cccaggtcga tgcgcagtgg cgcagcgtcg aattgccgcc gagctacctg cgcggcgcat
     1021 ccgtgctggt gagctgcgag accggcgaag aggtcatcaa gcgcatctgc gaagaccagc
     1081 tcggtgccga tctgcgcgcg cgcggcgtgc gcgtgctggt ggcggcgcgc ggcgcgttgc
     1141 cggccgtcgc acccgccggt ccgaccgacg cgcaatactt gccgctcgcg cgcgagaacg
     1201 gcgcgaaggc ggtgctcagt gtcagcgtcg gcccgagctc gcaaagcgtc agcccgggca
     1261 tctcgatcgg catcggcggc ttcggcttcg ggcgccattc ggccggcggc atcggcgtct
     1321 cggcgccgat cggaggcggg caggtcagcc agggctttgc tgcgaacggc cgcgtcaccg
     1381 atgtcgcgtc gggccgtctg atgtggacgg cgcgcgcgag ctcgccgccg tcgtcggacg
     1441 tgaacgcgca gatcgccgag ctgtcgacga gcgtgctcga cgcggccgca acggcggggc
     1501 tgttctgagg ctcaggccgc gatcgccccc ggccggctcg cctcgacgac gaagcgcgcc
     1561 gcggtcgtga tgccgctgcc cgggtcgagc gcatcgtcga cgacgcgcag ttgcgcctgc
     1621 agctgcttcg cgtcgagcgt gaagcgcacg tagccgcgct ggtcgctgcg cgcgtagcgc
     1681 aggtgcgggt tgaacgggcg catcgcgtcg agccgcgact gcgccatcga caggctcgtg
     1741 atcgaagtgc cgcagaactc gctcgcgatc accggggctt ccgcgtcgtc gaagtcggcc
     1801 ttcaggtcgg cgacgaagtt gctgtgcacg tcgccgccga gcacgacgac gccgggcacc
     1861 ttgcgctgcg cgaccacgcc gagcaggcgc tggcgcgcgg cggggtagcc gtcccagccg
     1921 tcggtccagt aggtgccgcc gccgttcgcc gcggccgtgc tcgtcaggcg cgccatcagt
     1981 gtctgctgcg cgagcaggtt ccaggggcgg tgcgtgtccc agccatcggc cagccattgc
     2041 tcctgcgcag cgccgagcag cgtgcgcttc gggtcgagca tctcggggca ggccttcagc
     2101 gcgaccgtgt tcgagccgcc gcctttcgcg ggcttcgggc agacctgcgg atcgcggtac
     2161 tggcggtcgt cgagcaggtg gatgcgcgcc agcaggcccc agtcgagccg gccggtgatg
     2221 cgcatgtcgg catcgagcgg gcgcgcggat ttgggaaacg gcatgtgctc ccagtacgcg
     2281 cggtaggcgg ccgcgcgctg cgccttgaag tcgggctcga ggtcctgccc ttgcaggccg
     2341 gcgtagtcgt tgccgacctc gtggtcgtcc cagaccagca accagggcac ggcggcgtgc
     2401 gcggcctgca gcgccgggtc gctct
//
LOCUS       DASRDG010000204         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_834424, whole genome shotgun sequence.
ACCESSION   DASRDG010000204 DASRDG010000000
VERSION     DASRDG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834424"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VFA35_03380"
     CDS             <1..677
                     /locus_tag="VFA35_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZT55242.1"
                     /translation="LVVGVLLAAGFGYLLEWAFFSFLYQRDHLQQVLMTYALILVFEE
                     ARSLLLGNDVHGVQAPSWLAGGFQLGALMSYPWYRVFASGACIVVALALYWVVNRTRL
                     GMMIRAGASNRDMARGLGVDIRRLYRVVFAGGVALAALAGMIAAPLSSVYPGMGGSVL
                     IVCFVVVVIGGIGSITGALIASLLVGFVDTFGKVFFAEYSGVGVYLLMAVVLVWRPEG
                     LMKRAC"
     gene            771..1718
                     /locus_tag="VFA35_03385"
     CDS             771..1718
                     /locus_tag="VFA35_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZT55243.1"
                     /translation="MRHPALTPIVAGAVLLALPPFLSPYLQDLVVRIAIYAVFALSLE
                     LLVGVAGLVSLGHAAFLGIGAYVTVLASGERGGSVATTLPLAMLAAAVYALFVGALSL
                     RTKGVYFIMVTLAFAQMAYFVFHDTKLAGGSDGLFLYAKPTLGALNLDDPRTMYYAAM
                     LALILTYALLALLMRARFGHALAGIRVNEQRMRAAGYPTYRYKLAAFVIAGALAGLAG
                     FLLAVKDGTVNPELLSWHESGAVLLMLILGGIGHLRGAVIGAIAFTLLKELFQLESLL
                     GPLAGHWQMTLGLTIIGFVALLPKGLIGLRQRLAGAAHG"
     gene            1711..>2277
                     /locus_tag="VFA35_03390"
     CDS             1711..>2277
                     /locus_tag="VFA35_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT55244.1"
                     /translation="MVEAVLSTNALTRRFGGVTAVDRVSIELRLGEVHALIGTNGAGK
                     STLIDMLSGELPASSGRVALDGRDIGTWPQPKRARAGIGRSYQRTTIFAEFSVLENCR
                     LAAQARAPRAWRIWEPAARDAEGIAVAQRALAAAGLDGDPKRVAATLSHGAKRQLEIA
                     MCLATEPRVLLLDEPLAGMGAEETDRMLE"
BASE COUNT          271 a          792 c          824 g          390 t
ORIGIN      
        1 ggctggtcgt cggggtgctg ctcgcggccg ggttcggcta cctgctcgag tgggcattct
       61 tcagctttct gtaccagcgc gaccacctgc agcaggtgct gatgacctat gcgctgatcc
      121 tggtgttcga ggaggcgcgc agcttgcttc tgggcaacga cgtgcacggc gtgcaggcgc
      181 cgtcgtggct cgcgggcggc tttcaactcg gcgcgctgat gtcgtacccg tggtaccggg
      241 tgttcgcctc cggcgcgtgc atcgtcgtcg cgctggcgct gtactgggtc gtgaaccgca
      301 cgcggctggg catgatgatc cgcgccggcg ccagcaaccg cgacatggcg cgcggcctcg
      361 gcgtcgacat ccggcggctg tatcgcgtcg tgttcgccgg cggcgtcgcg ctcgccgcac
      421 tggcggggat gatcgcggcg ccgttgtcgt cggtctatcc gggcatgggc ggcagcgtgc
      481 tgatcgtctg cttcgtcgtc gtcgtgatcg gcgggatcgg ctcgatcacc ggggcgctga
      541 tcgcgtcgtt gctggtcggc ttcgtcgaca ccttcggcaa ggtgttcttc gccgagtaca
      601 gcggggtcgg cgtgtacctg ctgatggcgg tggtgctggt gtggcggccc gaggggctga
      661 tgaagcgtgc ttgctgatcc ggctggcctc attcgacgct ccgagcgcgc ctggccgcgg
      721 gcgggtgcct gtggacgggc agcgctttgg aacgcgtgag caggcctgag atgcgccacc
      781 cggccctcac gccgatcgtc gcgggcgcag tgctgttggc cctgccgccg ttcctctcgc
      841 cctatctgca ggacctggtg gtcagaatcg cgatctacgc ggtcttcgcg ctgagcctcg
      901 agctgctcgt cggtgtggcc ggcctggtga gcctgggcca tgcggcgttc ctgggcatcg
      961 gcgcgtacgt gaccgtgctc gcgtcgggcg agcgcggcgg ctcggtcgcg acgacgctgc
     1021 cgctggcgat gttggcggcc gcggtctacg ccctgttcgt cggcgcgctg agcctgcgca
     1081 ccaagggcgt gtacttcatc atggtcacgc tcgcgttcgc gcagatggcc tacttcgtgt
     1141 ttcacgacac caagctggcc ggcggcagcg acggcctgtt cctctacgcc aagccgacgc
     1201 tcggcgcgct gaacctcgac gacccgcgca cgatgtacta cgcggcgatg ctcgcgctga
     1261 tcctcaccta cgcgctgctc gcgctgctga tgcgcgcgcg cttcggccac gcgctcgccg
     1321 gcatccgcgt caacgagcaa cgcatgcgcg ccgcgggcta cccgacctac cggtacaagc
     1381 tcgccgcgtt cgtgatcgcc ggcgcgctcg cggggctcgc gggctttctg ctcgcggtga
     1441 aagacggcac ggtgaacccc gagctgctga gctggcacga atcgggcgcg gtgctgctga
     1501 tgctgatcct cggcggcatc ggccacctgc gcggggcagt gatcggcgcg atcgcgttca
     1561 cgctgctgaa ggaactgttc cagctcgaat ccctgctcgg cccgctcgcg ggccactggc
     1621 agatgacgct ggggctgacg atcatcggct tcgtcgcgct gctgccgaag ggcctgatcg
     1681 ggctgcgcca gcggctcgcc ggggccgccc atggttgagg ccgtcctcag caccaacgcg
     1741 ctgacgcgcc gcttcggcgg cgtcaccgcc gtcgaccgcg tgtcgatcga gctgcgcctc
     1801 ggcgaggtcc acgcgctgat cggcaccaac ggcgccggca agtcgacgct gatcgacatg
     1861 ctgtcgggcg agctgcccgc gagcagcggc cgggtcgcgc tcgacggccg cgacatcggc
     1921 acatggccgc agccgaagcg cgcgcgcgcc ggcatcggcc gcagctacca gcgcacgacg
     1981 atcttcgccg agttcagcgt gctcgagaac tgccgcctcg ccgcgcaggc gcgcgcgccg
     2041 cgcgcctggc gcatctggga gccggcagcg cgcgacgccg aagggatcgc ggtcgcgcag
     2101 cgtgccctcg ccgccgccgg cctcgacggc gacccgaagc gcgtcgccgc gacgctcagc
     2161 cacggcgcca agcgccagct cgagatcgcg atgtgcctcg cgaccgagcc gcgcgtgctg
     2221 ctcctcgacg agccgctcgc cggcatgggc gcggaggaga ccgaccgcat gctggag
//
LOCUS       DASRDG010000205         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1179915, whole genome shotgun sequence.
ACCESSION   DASRDG010000205 DASRDG010000000
VERSION     DASRDG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179915"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFA35_03395"
     CDS             complement(<1..345)
                     /locus_tag="VFA35_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT55245.1"
                     /translation="MRRRTLLAGAALAPLAACRRAEPPGWGGDWVGANAPRGHRLRER
                     KAGGLLVPELTRRAGAIVVGAGIAGLAAARELHRAGIDDVQLLELEDEAGGNSRGHQI
                     AGMRCPLGAHYLP"
     gene            complement(345..1913)
                     /locus_tag="VFA35_03400"
     CDS             complement(345..1913)
                     /locus_tag="VFA35_03400"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HZT55246.1"
                     /translation="MTGTSGGAATDAAQVRPAEVALLASVFVVAACGLVYELAAGTLA
                     SYLLGDSILQFSTVIGSYLFAMGVGSYLSRFFERQLVAHFLRIELLVGLAGGLMPAAL
                     FALQSLASPSFRFALYAMVLLVGVLVGLEIPLVMRILKRQSGGGLKHLVSQVLTFDYL
                     GALAVSIAFPLLLVPQLGLIRTGAFFGLLNAGVAVWALWLFRGELRRVGAHATACALV
                     IAALLAAFAGADRVTTWAEDRFYADTVLFSESSKYQRVVVTASPQGVRLFLNGNLQFH
                     SRDEYRYHEALVHPAMAAHGAPKTVLVLGGGDGMAVREILKYPSVEQVTLVELDPRMT
                     QLFSTRPLLRQLNHDALLSPKLKIVNADAFAWLDDGVGKSAGSYDVIVVDFPDPTNFS
                     IGKLYTTSFYRLIDQHLGASGYAVVQTTSPLIARKSFWTVATTIEAAGLTATPYHAHV
                     PSFGEWGFIVASRRPYRLPATLPERLRFLSPADLPALFDFPLDMARVPTEVNRLSNQV
                     LVQTFEEEWGKVHQ"
     gene            complement(1924..2358)
                     /locus_tag="VFA35_03405"
     CDS             complement(1924..2358)
                     /locus_tag="VFA35_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HZT55247.1"
                     /translation="MIAVDTNLLVYAHRRESTFHDKALQAIAALAASGSAWAIPWPCL
                     HEFYGVVTNAHAFAPASTITQASTQLSAWFASPSLVMLHEGANHWAALHKLLVAGRVI
                     GGQVHDARIAAICIEHGVREFWSADRDFNRFPSLTVRNPLVG"
     gene            complement(2355..>2557)
                     /locus_tag="VFA35_03410"
     CDS             complement(2355..>2557)
                     /locus_tag="VFA35_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55248.1"
                     /translation="KIAARDGETLRSLVEQGLRKVVAERTAKAKPFKLRDGSVGTPGV
                     PSEYEKLSWEEKRALMYEGRGG"
BASE COUNT          421 a          894 c          899 g          343 t
ORIGIN      
        1 cggcaggtag tgcgcaccga gcgggcagcg catgccggcg atctggtggc cgcggctgtt
       61 gccgccggcc tcgtcttcga gttcgagcag ctgcacgtcg tcgatgccgg cgcggtgcag
      121 ttcgcgggcg gcggccaggc cggcgatccc ggcaccaacg acgatcgcgc cggcgcgacg
      181 cgtgagctcg ggcaccagca ggccgccggc cttgcgctcg cgcaggcgat ggccgcgcgg
      241 cgcgttcgcg ccgacccagt caccgcccca gcccggtggc tccgcgcgcc ggcaggccgc
      301 gagcggcgcg agcgccgcgc cggcgagcag cgtgcggcgg cgcatcactg gtggaccttg
      361 ccccattcct cttcgaaggt ctgcaccagc acctggttcg acaagcggtt cacctcggtc
      421 ggcacgcgcg ccatgtcgag cggaaagtcg aacagcgcag gcaggtcggc cgggctcaga
      481 aagcgcagtc gctcgggcaa ggtcgccggc aggcggtacg gccgccggct cgcgacgatg
      541 aagccccact cgccgaagct cggcacgtgc gcgtggtacg gcgtggccgt caggcccgcg
      601 gcttcgatcg tcgtcgcgac ggtccagaag ctcttgcgtg cgatcagcgg cgacgtggtc
      661 tgcaccaccg catagccgga ggcgccgagg tgctggtcga tcaggcggta gaagctcgtg
      721 gtgtagagct tgccgatcga gaagttggtc gggtcgggga agtcgaccac gatcacgtcg
      781 tagctgccgg cgctctttcc aaccccgtcg tccagccacg cgaacgcgtc ggcgttgacg
      841 atcttcagct tcggcgacag cagcgcgtcg tggttgagct ggcgcagcag cggccgggtc
      901 gagaacagct gcgtcatgcg cgggtcgagc tcgaccagcg tgacctgctc gaccgacggg
      961 tacttcagga tctcgcgcac cgccatgccg tcgcccccgc cgagcaccag cacggtcttg
     1021 ggcgcgccgt gcgccgccat cgccgggtgc accagcgcct cgtggtagcg gtactcgtcg
     1081 cgcgagtgga actgcaggtt gccgttcagg aacagccgca cgccctgcgg gctcgcggtg
     1141 acgacgacgc gctggtactt ggacgattcg ctgaacagca cggtgtcggc gtagaagcgg
     1201 tcctcggccc aggtggtgac gcggtcggca ccggcgaacg cggccaacag cgcggcgatc
     1261 acgagcgcgc acgcggtcgc atgcgcaccg acgcgccgca gctcgccgcg aaacagccac
     1321 agcgcccaga ccgcgacgcc cgcgttcagc agcccgaaga acgcaccggt gcggatcagc
     1381 ccgagctgcg gcaccagcag caacgggaac gcgatcgaga ccgcgagcgc gccgaggtag
     1441 tcgaaggtca gcacctgcga gaccaggtgc ttcagcccgc cgccggattg ccgcttgagg
     1501 atgcgcatca cgagtggaat ctcgagcccg accagcacgc cgaccagcag caccatcgcg
     1561 tacagcgcga agcggaacga cgggctggcg agcgactgca gcgcgaacag cgcggccggc
     1621 atcagcccgc ccgccaaccc gaccagcagc tcgatgcgca ggaagtgcgc gaccagttgc
     1681 cgctcgaaga agcgcgacag gtacgagccc actcccatcg cgaacaggta gctgccgatg
     1741 acggtcgaga actgcaggat cgagtcaccc agcaggtagc tcgcgagcgt gccggccgcg
     1801 agctcgtaga ccaggccgca ggcggcgacg acgaagacgg aagcgagcag cgcgacttcg
     1861 gcggggcgca cctgcgccgc atcggtcgcc gcgcccccgg aggtaccggt catgcgaatg
     1921 ccttcaaccc accagcgggt tgcgcaccgt caggctgggg aagcgattga agtcgcggtc
     1981 ggccgaccag aactcgcgca caccatgctc gatgcagatg gcggcgatgc gcgcgtcgtg
     2041 cacctgcccg ccgatcacgc ggccggcgac cagcagcttg tgcaaggccg cccagtgatt
     2101 cgccccctcg tgcagcatca cgagcgacgg cgacgcgaac cacgccgaca gctgcgtgct
     2161 cgcctgtgtg atggtcgagg ccggcgcgaa cgcgtgcgcg ttggtcacca cgccgtagaa
     2221 ctcgtgcagg cacggccacg ggatcgccca ggccgagccc gatgcggcga gcgcggcgat
     2281 ggcttgcagc gccttgtcat ggaaggtcga ttcgcgccgg tgcgcgtaga ccagcaggtt
     2341 ggtgtcgacg gcgatcaccc gccgcggccc tcgtacatca gcgcgcgctt ctcttcccag
     2401 gagagctttt cgtattcgga cggaacgccc ggcgttccga cgctgccgtc gcgcagcttg
     2461 aacggcttgg ccttggcagt tcgctccgcc acgaccttgc gcaggccttg ctcgacgagc
     2521 gagcgcaagg tctcaccgtc acgcgcagcg atcttcc
//
LOCUS       DASRDG010000206         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_955870, whole genome shotgun sequence.
ACCESSION   DASRDG010000206 DASRDG010000000
VERSION     DASRDG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955870"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFA35_03415"
     CDS             <1..814
                     /locus_tag="VFA35_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55249.1"
                     /translation="WVQERFAGYTALAAGLAFERHAASASRLERVALPPPPDAAQGAA
                     PAPAATGGDEIGDAVEYALVLPPMPGQPVRLGGRDDADPVAFAVDIAITDEFVDGSQR
                     SHKDADVVHLPETVVLGDRDHQEPLRLPVRLALADTGAVRRALQLTMALIPGYVGAEG
                     VRAPVRRTTIASVQFVQWPRGFQPIREKPLAALAAAQQLGDAAHFPHVRLAAEFAPPE
                     QRAEVEKRLIDWVRLGRPDQARVAMAALHALGVASVPIGDRDGWLAWWQARR"
     gene            825..2033
                     /gene="tyrS"
                     /locus_tag="VFA35_03420"
     CDS             825..2033
                     /gene="tyrS"
                     /locus_tag="VFA35_03420"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZT55250.1"
                     /translation="MTFPPVDEQLAVLRRGAVDLVHEDELRARLDASRRSGVPLRVKL
                     GIDPSSPDIHLGHTVVLRKLRDFQRLGHTAIVLWGTATAMVGDPTGRNKTRPPLTRAQ
                     VEANKRTYREQVAAVLDVRTAEERENGEWFDKATFMDGVALAGRQTVARVLERDDFQK
                     RHRDNQPISLHELLYPLMQGWDSVQLRCDVELGGTDQLFNLMVGRDLQKAEGQPPQVC
                     LTTPLLEGLDGSQKMSKSLGNYVGVAEPPGEKFQKLMRLPNGLLAKYFTLLTDLPSAD
                     AQALLARPLDAKFELARRIVAGYHGDAAAAAARAEFDRMHQKGGLPDEVPEVTAPADK
                     LKDGRLWLPAALALANLAASNSEGRRLVQGGGVRVGGEVCKDPDHALPPGRHLLQVGR
                     NKAAHVVVPG"
     gene            complement(2073..>2699)
                     /locus_tag="VFA35_03425"
     CDS             complement(2073..>2699)
                     /locus_tag="VFA35_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HZT55251.1"
                     /translation="ARAADGPRVTAYEPMLLQGSETKADAGQDEGARVTILKHRKFVA
                     DDGVLEKMADRSQPAGASYVGSDKCAGCHLADFTAWKASKHADAWQVLEKAEMDRAWP
                     VTRYPDCVSCHVVGYRKPGGFVSAKETPLLVNVGCERCHGPGSAHVDSGGATRLGKVA
                     GDLPKSTLCTQCHDFEQTPKFDYTSFWAIIQHGAKATPPKKPLKLGGR"
BASE COUNT          360 a          997 c          980 g          362 t
ORIGIN      
        1 ctgggtgcag gagcgcttcg ccggctacac ggcgctcgcc gcgggactcg cgttcgaacg
       61 ccacgcggcc tccgccagcc ggctcgaacg ggtggcgctg ccgccaccgc ccgacgccgc
      121 acagggcgcc gcgccggcgc cggcggcgac gggcggggac gagatcggcg atgccgtcga
      181 gtatgcactg gtgctgccgc cgatgccggg gcagccggtg cggctcggcg gccgcgacga
      241 cgcggatccg gtcgcgttcg cggtcgacat cgcgatcacc gacgagttcg tcgacggcag
      301 ccagcgcagc cacaaggacg cggacgtcgt ccacctgccg gagacggtgg tgctcggcga
      361 ccgcgaccac caggagccgc tgcggctgcc ggtgcggctc gcgctcgcgg acaccggcgc
      421 ggtgcgccgc gcgctccagc tcaccatggc gctgatcccc ggatacgtcg gcgccgaggg
      481 cgtgcgcgcg cccgtgcggc gcacgacgat cgcgtcggtg cagttcgtgc agtggccgcg
      541 aggcttccag ccgatccgcg agaagccgct ggcggcgctc gccgccgcgc agcagctcgg
      601 cgatgccgcg cacttcccgc acgtgcggct cgcagcggag ttcgcgccgc ccgagcagcg
      661 cgccgaggtc gagaagcggc tcatcgactg ggtgcgcctc ggccgccccg accaggcacg
      721 cgtcgcgatg gccgcgctgc acgcgctcgg cgtcgcgtcc gtgccgatcg gcgatcgcga
      781 cggctggctt gcctggtggc aggcacgccg ctagttctct cggcgtgacg ttcccacccg
      841 tcgacgagca gctggccgtg cttcgccgcg gcgccgtcga cctcgtgcac gaagacgaac
      901 tgcgcgcccg cctcgacgcc agccgccgca gcggcgtgcc gctgcgcgtg aagctcggca
      961 tcgatccgag ctcgcccgac atccacctcg ggcacacggt cgtgctgcgc aagctgcgcg
     1021 acttccagcg cctcggccac acggcgatcg tgctgtgggg caccgcgacg gcgatggtcg
     1081 gcgacccgac cgggcgcaac aagacgcggc cgccgctcac gcgcgcgcag gtcgaggcga
     1141 acaagcgcac ctaccgcgag caggtcgcgg cggtgctcga cgtgcgcacg gccgaggagc
     1201 gcgagaacgg cgagtggttc gacaaggcga cgttcatgga cggcgtcgcg ctcgcgggcc
     1261 gccagaccgt cgcgcgcgtg ctggagcgcg acgacttcca gaagcgccac cgcgacaacc
     1321 agccgatctc gctgcacgaa ctgctctacc cgctcatgca gggctgggac tccgtgcagc
     1381 tccggtgcga cgtcgagctc ggcggcaccg accagctgtt caacctgatg gtcgggcgcg
     1441 acctgcagaa ggcggaaggc cagccgccgc aggtctgcct gacgacgccg ctgctggaag
     1501 gcctcgacgg cagccagaag atgtcgaagt cgctcggcaa ctacgtgggc gtcgccgagc
     1561 cgccgggcga gaagttccag aagctgatgc gcctgccgaa cgggctgctc gcgaagtact
     1621 tcacgctgct caccgacctg ccctccgccg acgcgcaggc gctgctcgcg cgcccgctcg
     1681 acgcgaagtt cgagctcgcg cggcgcatcg tcgcgggcta ccacggcgac gccgcggccg
     1741 cggccgcgcg cgccgagttc gaccgcatgc accagaaggg cggcctgccg gacgaggtgc
     1801 ccgaggtgac ggcgcccgcc gacaagctga aggacggccg gctctggctg cccgcggcgt
     1861 tggcgctcgc gaacctcgcg gcgtcgaaca gcgagggccg gcggctcgtg cagggcggcg
     1921 gcgtgcgggt cggcggcgag gtgtgcaagg accccgacca cgcgctgccc cccggtcggc
     1981 acctgctgca ggtcggcagg aacaaggccg cgcacgtggt ggtgcccggc tgagcgcgag
     2041 gatcgcggcc tgcgggccgc gttcgcggcc ggctacctgc cgccgagctt gagcggcttc
     2101 ttcggcggcg tcgccttggc gccgtgctgg atgatcgccc agaagctcgt gtagtcgaac
     2161 ttcggcgtct gctcgaagtc gtggcactgc gtgcacagcg tcgatttcgg caggtcgccg
     2221 gcgaccttgc cgagcctggt cgcgccaccg ctgtcgacgt gcgcactgcc cggaccgtgg
     2281 cagcgctcgc agccgacgtt caccagcagc ggcgtctcct tcgccgagac gaagccgccc
     2341 ggtttgcggt agccgaccac gtgacagctc acgcagtcgg gatacctggt gaccggccag
     2401 gcgcgatcca tctccgcctt ctcgagcact tgccaggcat ccgcgtgctt gctcgccttc
     2461 cacgccgtga agtccgccag gtgacagccc gcgcacttgt cgctgccgac gtagctcgcg
     2521 cccgcgggct ggctgcggtc ggccatcttc tcgagcacgc cgtcgtcggc gacgaacttg
     2581 cggtgcttca ggatggtcac gcgcgccccc tcgtcctggc cggcatcggc cttggtctcg
     2641 ctgccctgca gcagcatcgg ctcgtaggcg gtgacacgcg gtccgtcggc ggcgcgcgc
//
LOCUS       DASRDG010000207         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_261946, whole genome shotgun sequence.
ACCESSION   DASRDG010000207 DASRDG010000000
VERSION     DASRDG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261946"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..528
                     /locus_tag="VFA35_03430"
     CDS             79..528
                     /locus_tag="VFA35_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZT55252.1"
                     /translation="MTPADLAGQVGQEAGVSAWITLDQQRIDEFAHCTGDDQWIHVDV
                     ERAVKESPFGGTIAHGFLTLSLLPVTGYEVLFKTLVVKQALNYGVEKVRFIAPVRAGK
                     RVRNRIKIAALEDKGGGRWLLTTENTIEIEGEGKPALVATALAMLMV"
     gene            561..2480
                     /locus_tag="VFA35_03435"
     CDS             561..2480
                     /locus_tag="VFA35_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZT55253.1"
                     /translation="MSKQIDARTPAARSAPRRSAVKKAAKTAAVPRRGAVKKAAQAAP
                     APRRPKAAAAEAASDRGAAADMPRAKAAAVAEAAAPRADDLAATASANTLTFNPLIGL
                     RADDISAGAGSLLKMVAASPRGAAQQYGRYLSELASILGGKSELAADPKDKRFADPGW
                     KSNPFYKALMQGHLAAQKELGAYIASSDSDKREKGRAQFFASLVTDALAASNWLFGNP
                     AAVRKIVDTGGKSLVDGAKNMLTDLREKRSLPASVDASGFKVGENVATTPGQVVLRIE
                     QLELIQYTPTTPQVYRRPIVMVPPQVNKFYAFDLSPEKSLLKWALDSGLQVFVISWRN
                     PTAEQRDWGIDTYAMAIDQAVDAAREITGSPDVNIWGSCSGGMTLAAYLGYLAGIGDR
                     KVANSSWAVCILDTQSATDETTLGLFSSPATIAAARKHSASKGVVDGAEMATMFAWLR
                     PNDLIWNYWVNNYLLGNKPPAYDVLAWNADTTRLPARFHSDLLGLCERNPYLEAGAFE
                     VAGVPIDLSKLDIGAYVIGGITDHITPWKGCYGTARLLGEDSTFVLANAGHLQSLLNP
                     PGAPKSFFFTAPASVPDPDEWAKSATRHEGSWWPHWREWMQQRSSELVNAPAQLGSRK
                     HKPLVAAPGEYVHVK"
     gene            2522..>2789
                     /locus_tag="VFA35_03440"
     CDS             2522..>2789
                     /locus_tag="VFA35_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZT55254.1"
                     /translation="MPTEIRMVEVDGQPLRVGVRSGTQEGPPLLIFNGIGANLELVEP
                     FTAALAGVETVVFDVPGAGGSPAPAWPYRFFHLARLADRLMTQLG"
BASE COUNT          485 a          974 c          957 g          373 t
ORIGIN      
        1 agcctgcgtc atccaccacc ccgaagggaa accggacatg agaacgaaca tcccctgctc
       61 taaaccgagg ggcacaccat gaccccggcc gatctggccg gccaggtggg gcaggaggcc
      121 ggcgtatcgg cctggatcac gctggaccag cagcgcatcg acgagttcgc gcattgcacc
      181 ggcgacgacc agtggatcca cgtcgatgtc gagcgcgctg tcaaggagag tccgttcggc
      241 ggcaccatcg cgcacggctt cctgacgctg tcgctgctcc cggtgaccgg ctacgaggtg
      301 ctgttcaaga cgctggtggt gaagcaggcg ctgaactacg gcgtcgagaa ggtacgcttc
      361 atcgcgccgg tgcgagccgg caagcgcgtg cgcaaccgca tcaagatcgc cgcgctcgag
      421 gacaagggcg gcggtcgctg gctgttgacg accgagaaca ccatcgagat cgagggcgag
      481 ggcaagccgg cgctggtcgc caccgcgctg gccatgctga tggtttgacg ccaggccccc
      541 acgcagaaca ggagacacga atgagcaagc agattgacgc gcgcacgccg gcagcgcgct
      601 ccgcgccgcg ccgcagcgcc gtgaagaagg ccgccaagac cgcagcggta ccgcgacgcg
      661 gtgccgtgaa gaaggccgcc caggccgcgc cggcaccgcg gcgcccgaag gcggcggccg
      721 ccgaagccgc ctcggaccgg ggcgcggcgg ccgacatgcc gcgcgccaag gccgctgcgg
      781 tggccgaggc cgccgcgccc cgcgccgacg acctggccgc gaccgccagc gcgaacacgc
      841 tgaccttcaa cccgttgatc gggctgcggg cagacgacat cagcgccggc gcgggctcgc
      901 tgttgaagat ggtcgccgcg tcgcctcggg gcgcggccca gcagtacggc cgttatctga
      961 gcgagctcgc cagcatcctc ggcggcaagt cggagctcgc ggccgatccg aaggacaagc
     1021 gttttgccga cccgggctgg aagtcgaacc ccttctacaa ggcgctgatg cagggccatc
     1081 tggccgcgca aaaggaactc ggcgcctaca tcgcgtcgag cgactcggac aagcgcgaga
     1141 aggggcgggc gcagttcttc gcgtcgctcg tcaccgacgc gctggcggcg agcaactggc
     1201 tgttcggcaa cccggcggcg gtgcgcaaga tcgtcgacac cggcggcaag agcctggtcg
     1261 acggcgccaa gaacatgctg accgacctgc gcgagaagcg cagcctgccc gcgtcggtcg
     1321 acgccagcgg gttcaaggtc ggcgagaacg tcgccaccac accgggccag gtggtgctgc
     1381 gcatcgagca actcgaattg atccagtaca cgcccaccac gccgcaggtg tatcgccggc
     1441 cgatcgtgat ggtgccgccg caggtgaaca agttctacgc cttcgacctg tcgccggaga
     1501 agagcctgct caagtgggcg ctcgacagcg gcctgcaggt gttcgtgatc agctggcgca
     1561 atccgaccgc cgagcagcgc gactggggca tcgataccta cgcgatggcc atcgaccagg
     1621 cggtggacgc cgcgcgcgag atcaccggca gcccggacgt caacatctgg ggctcctgct
     1681 cgggcggcat gacgctggcc gcctacctgg gttacctggc cggcatcggc gaccgcaagg
     1741 tcgccaactc gagctgggcg gtgtgcatcc tcgatacgca gtccgcgacg gacgagacca
     1801 cgctggggct cttcagttcg ccggccacca tcgccgccgc acgcaagcac tcggccagca
     1861 agggcgtcgt cgacggcgcc gagatggcga ccatgttcgc gtggctgcgg ccgaacgacc
     1921 tcatctggaa ctactgggtc aacaactacc tgctcggcaa caagccgccg gcctacgacg
     1981 tgctggcctg gaacgccgac acgacgcggc tgccggcgcg cttccactcc gacctgctcg
     2041 gcttgtgcga gcgcaacccc tacctcgagg ctggagcgtt cgaggtggcc ggggtgccga
     2101 tcgacctgtc caagctggac atcggcgcct acgtgatcgg cggcatcacc gaccacatca
     2161 caccctggaa aggctgctac ggcacggcgc ggctgctggg cgaggactcg accttcgtgc
     2221 tcgccaatgc cggccacctg caaagcctgc tcaacccgcc cggcgcgccg aagtccttct
     2281 tcttcaccgc gccggccagc gtgcccgacc cggacgagtg ggccaagagc gcgacgcgcc
     2341 acgaaggcag ctggtggccg cactggcgcg aatggatgca gcagcgatcg agcgagctcg
     2401 tcaacgcgcc cgcgcagctc ggctcgcgca agcacaagcc gctggtcgcc gcaccgggcg
     2461 aatacgtgca cgtcaagtga actccgatgc cgatcgacgc tcgagcgtgg gcagcacagt
     2521 gatgccgacc gaaatccgca tggtcgaggt ggacgggcag ccgctgcgtg tcggcgtgcg
     2581 ctccggcacg caggagggac cgccactgct gatcttcaac ggcatcggcg cgaacctcga
     2641 gctggtggag ccgttcaccg ctgcgctggc cggcgtcgag accgtggtgt tcgacgtgcc
     2701 cggcgccggc ggctcgcccg cgccggcgtg gccctatcgc ttcttccacc tcgctcggct
     2761 ggccgaccgg ctgatgacgc agctgggct
//
LOCUS       DASRDG010000208         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_835308, whole genome shotgun sequence.
ACCESSION   DASRDG010000208 DASRDG010000000
VERSION     DASRDG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835308"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2223
                     /locus_tag="VFA35_03445"
     CDS             <1..>2223
                     /locus_tag="VFA35_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZT55255.1"
                     /translation="NIRDFAGDGLFTAKTEEPNWAVAHRLLMPAFGPIGMRGMFDRML
                     DVADQMVTRWERFGPANVIEVTEDMTRMTLDTIALCAFDYRFNSFYQREMHPFVDAMV
                     DALSEAGARTRRPEIASKLLLRTRRAYEDDLALMRNIADELIAERKRDPEGAQKKDLL
                     GIMLQGRDPVTGEGLSDENIRFQLITFLIAGHETTSGMLSFATYFLVKNPQVLAKARA
                     EVDAVLGTEMPRVEQIGKLRYIEQVLMEALRIWPTAPAFALRANENTVIGGRYAISRR
                     DTLMVLSPMLHRDPKVWGEDVEAFRPERFAPDEMAKLPPNAWKPFGNGQRACIGRPFA
                     MQEAILLVAMILQRFDLIEDDPSYQLKVAETLTLKPAGFRIRVKRRGSASFKPRTVLA
                     PQPAAAPAAAPAAAPALAPGEQAMPLLVLYGSNTGSSEAFADRIASEAALQGYAAQAA
                     PLDDYTGGLPNEGAVVIITASYEGQPPDNARQFAAWLDNLAPDALKGVRYAVFGCGNR
                     QWARTYQAVPQRFDAALEAAGATRLRPRGETDAGGDFFGAFDEWYAGLWTDFGRALGK
                     QVQEAPGGVQLQVERVTSGRAALLRLGDLQHGEVVVNRELVDMKSPLGRSKRHIEIAL
                     PAGMSYRAGDYLSVLPHNPIGVVMRALKRFDLASDSQIIIRKPESSLTSLPVNYPVNA
                     SELLFHYVELAQPATRGQVAALADATTGPQQAELAHLAEDAAYQRDVLAKRVSVLELL
                     E"
BASE COUNT          352 a          807 c          762 g          302 t
ORIGIN      
        1 aacatccgcg acttcgccgg cgacggcctg ttcaccgcca agaccgagga gcccaactgg
       61 gccgtggcgc accggctgct gatgccggcc ttcggcccga tcggcatgcg cggcatgttc
      121 gaccgcatgc tcgacgtcgc cgaccagatg gtcacgcgct gggagcgctt cggcccggcc
      181 aacgtgatcg aggtgaccga ggacatgacg cgcatgaccc tcgacacgat cgcgctgtgc
      241 gccttcgact accgcttcaa cagcttctac cagcgcgaga tgcacccctt cgtcgacgcg
      301 atggtcgacg cgctctcgga ggccggcgcg cgcacgcggc ggcccgagat cgcgagcaag
      361 ctgctgctgc gcacacgccg cgcctatgag gacgacctgg cgctgatgcg caatatcgcc
      421 gacgagctga tcgccgagcg caagcgcgac cccgaggggg cgcagaagaa ggacctgctc
      481 ggcatcatgc tgcaggggcg cgacccggtc accggcgagg gcctctcgga cgagaacatc
      541 cgcttccagc tgatcacctt cctgatcgcc ggccacgaga ccaccagcgg catgctgtcg
      601 ttcgcgacct acttcctggt caagaacccg caggtgctgg ccaaggcgcg cgccgaggtc
      661 gacgcggtgc tgggcaccga gatgccgcgc gtcgagcaga tcggcaagct gcgctacatc
      721 gagcaggtgc tgatggaggc gctgcgcatc tggcccacgg cgccggcgtt cgcgctgcgc
      781 gccaacgaga acaccgtgat cggcggacgc tacgcgatca gccgacgcga cacgctgatg
      841 gtgctgtcgc cgatgctgca ccgcgacccc aaggtctggg gcgaggatgt cgaggccttc
      901 cgccccgaac gcttcgcgcc ggacgagatg gccaagctgc cgcccaacgc ctggaagccc
      961 ttcggcaacg gccagcgcgc ctgcatcggc cggcccttcg cgatgcagga ggcgatcctg
     1021 ctggtggcga tgatcctgca gcgcttcgac ctgatcgagg acgacccctc ctaccagctc
     1081 aaggtggccg agacgttgac gctcaagccc gcgggctttc gcattcgcgt caagcgccgc
     1141 ggcagcgcgt cgttcaagcc gcgcacggtg ctcgcgccgc agccggccgc tgcgccggcg
     1201 gccgcaccgg ccgccgcccc ggcgctggcg ccgggcgagc aggccatgcc gctgctggtg
     1261 ctgtacggct ccaacaccgg ctcgtccgag gcctttgccg atcgcatcgc cagcgaggcc
     1321 gccctgcaag gctacgcggc gcaggcggcg ccgctggacg actacacggg cgggctgccc
     1381 aacgaaggcg cggtggtgat catcaccgcc tcctacgaag gccagccgcc cgacaacgcg
     1441 cgccagttcg ccgcctggct cgacaacctg gcgcccgatg cgctgaaagg cgtgcgctac
     1501 gccgtgttcg gctgcggcaa ccgccaatgg gcacgcacct accaggcggt gccgcagcgc
     1561 ttcgacgccg cgctggaagc cgccggcgcc acccggctgc gcccgcgcgg cgaaaccgac
     1621 gccgggggcg acttcttcgg cgccttcgac gagtggtacg ccgggctctg gaccgatttc
     1681 ggccgggcgc tgggcaagca agtgcaggag gcgcccggcg gcgtgcaact gcaggtggag
     1741 cgggtcacga gcggccgcgc cgcactgctg cggctgggcg atctgcagca cggcgaggtg
     1801 gtcgtgaacc gcgaactggt cgacatgaag tcgccgctgg gccgctccaa gcgccacatc
     1861 gagatcgcgc tgcccgccgg catgagctac cgcgccggcg actacctctc ggtgctgccg
     1921 cacaacccga tcggcgtggt gatgcgcgcg ctcaagcgct tcgacttggc gtccgacagc
     1981 cagatcatca tccgcaagcc cgagagcagc ctgacctcgt tgccggtgaa ctacccggtc
     2041 aacgccagcg agttgctgtt ccattacgtc gagctggcgc agccggccac gcgcggccag
     2101 gtggctgcgc tcgccgacgc caccacgggc ccgcagcagg ccgagctggc gcatctggcg
     2161 gaggacgcgg cctaccagcg cgacgtgctc gccaagcgcg tcagcgtgct cgagctgctg
     2221 gag
//
LOCUS       DASRDG010000209         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1637127, whole genome shotgun sequence.
ACCESSION   DASRDG010000209 DASRDG010000000
VERSION     DASRDG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637127"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFA35_03450"
     CDS             <1..573
                     /locus_tag="VFA35_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HZT55256.1"
                     /translation="WNMTTRSTPKPDRMIFDLDPGEGVAWPAVREAAQLMKSFLDELG
                     LKSLLKTSGGKGLHVVVPLAPKHTWQAVRQFSQAIVVHMAQVVPQRFVAKSGPRNRVG
                     KIFIDYLRNGWGATPASAWSARARPGLGVSVPIDWSELAQVDGGAQWNVRNIAPRLAV
                     GNAPWGAFASSRQSLAAAMKALGVATEDVS"
     gene            811..1137
                     /locus_tag="VFA35_03455"
     CDS             811..1137
                     /locus_tag="VFA35_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HZT55257.1"
                     /translation="MPRNPWSAKRERQYEHIKQDLLERGKGTKLAEEIAARIVNKERA
                     QHGEAQAASPSSVNDISAGRRGGLRSHNGAAGRTLAQLRHEASDRGVKGRSKMNKAQL
                     EAALKR"
     gene            1396..2046
                     /locus_tag="VFA35_03460"
     CDS             1396..2046
                     /locus_tag="VFA35_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZT55258.1"
                     /translation="MPRGRAPGYDTQRGQILARAAELFARQGYTGTSMNQVALACGVS
                     KPSLYHYVRDKYQLLVEIAEDHVGRLEVLARQAPPRPLEPQARVRALIGSFLEVYADA
                     QAAHRVLIEEVKFLEAADRERVLDRQRKVVAAFAEAIAEARPELRATELHKPLTMLLF
                     GMMNWLFTWLQPGGRLSYAAIAPIVADLFFGGLNAVRLAEPALAPIAVKPPRRRHP"
     gene            2043..>2740
                     /locus_tag="VFA35_03465"
     CDS             2043..>2740
                     /locus_tag="VFA35_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT55259.1"
                     /translation="MKLKQSLIALAAAVAAASLAHADVVVGVTVSATGPAASLGIPEK
                     NTIALMPQTIGGQKINYIVLDDATDTTLAVSNTRKLITESKADIILGSTVTPNSLAMI
                     DVVAENQTPMISMAASARIIEPMDAKRRWVFKTPQNDIMMSLAIAASMADSGVKTVGF
                     IGFADAYGEGWYQEFAKAAALKRLTIVASERYARADTSVTGQVLKIVAAKPDAVLIAG
                     SGTPAVLPQRTLKER"
BASE COUNT          459 a          957 c          939 g          385 t
ORIGIN      
        1 tggaacatga cgacgcgctc gaccccgaag cccgaccgca tgatcttcga cctcgacccg
       61 ggcgagggcg tcgcgtggcc ggccgtgcgt gaagcagcgc agttgatgaa gtccttcctc
      121 gacgaactgg gcctgaagag cctgctcaag accagcggcg gcaagggcct gcacgtggtc
      181 gtgccgctcg cgccgaagca cacgtggcag gcggtgcggc agttctcgca ggcgatcgtg
      241 gtgcacatgg cgcaggtcgt gccgcagcgt ttcgtcgcga agagcgggcc gcgcaaccgc
      301 gtcggcaaga tcttcatcga ctacctgcgc aacggctggg gagcgacccc cgcctcggcc
      361 tggtcggcac gcgcgcggcc ggggctgggc gtctcggtgc cgatcgattg gagcgagctg
      421 gcgcaggtcg atggcggcgc gcagtggaac gtgcgcaaca tcgcgccgcg gctcgcggtc
      481 ggcaacgcac cgtggggcgc gttcgcgtca tcgcggcaat cgctcgcggc ggccatgaag
      541 gcgctcggcg tcgcgacgga ggacgtttca tgatgatcca gttcgaccac atcgagccgc
      601 cggtgatccc ggacacccac ccgaatcgcc cgccgccggc accggcgccc gacgtcgtgc
      661 cggtgcgcga tccggtgctc cccgagcacc ccgatccggt gcgtgagccg ccgacgcacg
      721 ggccgccgat ttccacctcc tggccgcagc gcggggcccg gcagccgcct cgtgcactcg
      781 cgatgtcatc cgcctgccga aggagcgaac atgccccgca acccatggag cgcgaagcgc
      841 gaacgccaat acgaacacat caagcaagat ctgctcgaac gcggcaaggg cacgaagctc
      901 gccgaggaaa tcgcggcccg catcgtcaac aaggagcgcg cgcagcacgg tgaagcgcag
      961 gcggcaagcc cgtcgtcggt caacgacatc tcggcgggcc ggcgcggtgg tctgcgctcg
     1021 cacaacggcg ctgccggccg cacgctcgcg caactgcgcc acgaggccag cgatcgcggc
     1081 gtgaaggggc gctcgaagat gaacaaggcg cagctcgagg ctgcgctgaa gcgctgaagc
     1141 gctgaagggc gtcccgtccc ttccccttcc aatagacccg cgggtaagcc ctgggctgcg
     1201 gcgcgctcga acgcgccgga ttcccactag acttttactg accggtcggt cgagtaagtt
     1261 tgtccgtgtg ggacaccggc acgcaggaga cccgatggca gaacccaagg cgctgagcag
     1321 gctcgccgcg cagcgcgacg acgacgtcgt agccgcatgc atggcgcggc ggcccgcggc
     1381 gttctcgggc cgctgatgcc gcgcggccgc gcccccggct acgacacgca gcgcgggcag
     1441 atcctcgcgc gcgcggcaga gttgttcgcg cggcagggct atacagggac ctcgatgaac
     1501 caggtcgcgc tcgcctgcgg cgtctccaaa ccctcgctgt atcactacgt gcgcgacaaa
     1561 taccagttgc tggtcgagat cgccgaagac catgtcggtc ggctcgaggt gctggcgcgg
     1621 caggcgccgc cgcggccgct cgagccgcag gctcgtgtac gcgcgttgat cggcagcttt
     1681 ctcgaggtct acgccgacgc gcaggccgcg caccgcgtgc tgatcgagga ggtgaagttt
     1741 ctcgaggcgg ccgaccgcga gcgcgtgctc gaccgccagc gcaaggtcgt cgcggccttc
     1801 gccgaggcga tcgccgaggc gcgccccgag ttgcgagcga ccgagctgca caagccgctc
     1861 acgatgctgc tgttcggcat gatgaactgg ttgttcacct ggctgcagcc cgggggcagg
     1921 ctgagctacg cggcgatcgc gccgatcgtt gccgacctct tcttcggcgg cttgaatgcg
     1981 gtgcgcctgg ccgaacccgc tctcgccccg atcgctgtga aacccccaag acggagacat
     2041 ccatgaagct caagcagtcc ctcatcgcgc tcgccgccgc ggtcgccgcc gcctcgctcg
     2101 cgcatgccga cgtggtcgtg ggcgtgaccg tctcggccac cggcccggcg gcctcgctcg
     2161 gcatccccga gaagaacacg atcgcgctga tgccgcagac gatcggcggc cagaagatca
     2221 actacatcgt gctcgacgac gccaccgaca cgacgctggc ggtcagcaac acccgcaagc
     2281 tgatcaccga gagcaaggcc gacatcattc tcggctcgac cgtgacgccg aactcgctcg
     2341 cgatgatcga cgtggtggcc gagaaccaga cgccgatgat ctcgatggcc gcgtcggcgc
     2401 gcatcatcga gccgatggac gcgaagcgcc ggtgggtctt caagacgccg cagaacgaca
     2461 tcatgatgtc gctcgcgatc gccgcgagca tggccgacag cggcgtcaag acggtcggtt
     2521 tcatcggctt cgccgacgcc tatggcgaag gctggtacca ggagttcgcg aaggctgccg
     2581 cgctgaagcg gctgacgatc gtcgccagcg agcgctacgc gcgcgccgac acctcggtca
     2641 ccggccaggt gctgaagatc gtcgccgcca agcccgatgc ggttctcatc gccggctcgg
     2701 gcacgcccgc ggtgctgccg cagcgcacgc tgaaggagcg
//
LOCUS       DASRDG010000210         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_392256, whole genome shotgun sequence.
ACCESSION   DASRDG010000210 DASRDG010000000
VERSION     DASRDG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392256"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..>2916
                     /locus_tag="VFA35_03470"
     CDS             58..>2916
                     /locus_tag="VFA35_03470"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZT55260.1"
                     /translation="MLLAAYYLSGRVGLSVPQFGTYITLIWPPAGIALAALLRFGLGV
                     WPGVWLGAFAIALSLGTSLWLAVLIAFGNTLGPLLGAWVLQRDGLHAELDRRRDLWLF
                     GSVGIGLSTLITASNGAFWLAVAGRIAWADLPRAWMYWWLGDAMGALVVGIPLLTLSR
                     AALHQALGEWRWVPAVLLALGTIASAALAALFAGGEHAVRSPLFFAPHLLLCVMAARS
                     GLFAASTTALLLAAGTVLATVHGLGPFHQPDGSQGVSLLVGYVCSLLAIPLLTTALNG
                     EIAANERRWQLALDASQIGIGEWDLRTRRVEFSPRWLALLGYSTQGFGDTLHAFWALI
                     HRDDVTSVQRAFEPLRAAGAINCRAECRMQCRDGSWRLFELHALVAERGPSGEPLRII
                     STARDISDIQAARERQDLSQTVFQHLHEGLLITDSQHRVLESNPTFSEITGYTRDELL
                     GGVPVLLQAHASDTQLTLQLEAMQLGLADEGSWRGELNHHRRNGEPCVLQLTVTAVRN
                     ANGSVRNHVLAIADVTHARAQFEQLQRQAHFDELTRLPNRVRLARMLHSAMQTSRREG
                     SLLTVCYLDLDHFKPVNDRFGHEAGDRLLVELAGRMKRSLRSWAGGDDVVARIGGDEF
                     VLLLRTATLEESRHAVERVLNQVSQPYGLGVGAGPVMVTASIGATVFPLDGADAETLL
                     RHADHAMYGAKQAGRNGYLFFDAEHDRRAEARFVALGRVQEALDANEFRLYFQPKVDM
                     RNAKVLGVEALLRWKHPEQGVISPAQFLPLIENTGLAISVGNWVLQQGIDQLARWLRL
                     GLDITVSINVSARHLQEPLFAQHLAGLLSRHQAPVAHRLIIEVLETAALADVDYTCEL
                     MEECRALGVRFALDDFGTGYSTFTYLKRLPIDMLKIDRSFVINMLGDRQDLAIVEGVI
                     GLSHTFGCSVVAEGVETRAQAERLIAIGCAIGQGNGIAA"
BASE COUNT          390 a         1040 c         1018 g          468 t
ORIGIN      
        1 tacctccccg cgatcgcctc gaccgtcggc tcacgcgact gggcttcgcg caccgcattg
       61 ctgctggcgg cctactacct gagcggccgg gtcggtctga gcgtcccgca gttcggcacg
      121 tacatcacgc tgatctggcc cccggccggc attgcgctgg cggccttgct gcgcttcggg
      181 ctcggcgtgt ggccgggggt gtggctcggg gcattcgcga tcgcgctgtc gctcggcacg
      241 tcgctctggc tcgcggtgct gattgccttc ggcaacacgc tcgggccgct gctcggcgcc
      301 tgggtgctgc agcgcgatgg cctgcatgcc gagctcgatc ggcgccgcga tctgtggctg
      361 ttcgggtcgg tcggcatcgg cctctcgacg ctgatcaccg ccagcaacgg cgcgttctgg
      421 ctcgccgtcg ccggccggat cgcgtgggcc gatctgccgc gcgcctggat gtactggtgg
      481 ctcggcgacg cgatgggcgc gctggtggtc ggcattccgc tgctgacgct gtcgcgcgcg
      541 gcgctgcatc aggcccttgg cgagtggcgc tgggtgcccg ccgtgctgct tgcgctgggc
      601 acgatcgcca gcgcggcgct ggccgcgctg ttcgccggcg gcgagcacgc ggtccggtcg
      661 cccctgttct tcgcgccgca cctgttgctg tgcgtcatgg cggcgcgcag cgggctgttc
      721 gcggcgtcga cgacggcgct gctgctcgcc gccggcacgg tgctggccac ggtgcatggg
      781 ctcgggccgt tccatcaacc ggacggcagc caaggtgtct cgctgctcgt cggctatgtc
      841 tgcagcctgc tcgcgatccc gctgctgacg acggcgctga acggcgagat cgccgccaac
      901 gagcggcgct ggcaactcgc gctggacgcc tcgcagatcg gcatcggcga gtgggatctg
      961 cgcacccgcc gcgtcgagtt ctcgccgcgc tggctggcct tgctcggcta ctcgacgcaa
     1021 ggcttcggcg atacgctgca tgcgttctgg gcgctgattc accgcgacga cgtgacgtcg
     1081 gtgcaacgcg cgttcgagcc gctgcgcgcc gccggcgcga tcaactgccg cgccgaatgc
     1141 cggatgcaat gccgcgacgg cagctggcgc ctgttcgagc tgcatgcgct ggtggccgag
     1201 cgcggcccga gcggcgagcc gctgcgcatc atcagcaccg cgcgcgacat cagcgacatc
     1261 caggccgcgc gcgaacggca agacctgtcg cagacggtgt tccagcacct gcacgagggc
     1321 ctgctgatca ccgactcgca gcatcgcgtg ctcgaatcga acccgacctt cagcgagatc
     1381 accggctaca cgcgcgacga actgctcggc ggcgtgccgg tgctgctgca ggcgcatgcg
     1441 tccgacacgc agctcacgct gcaactcgaa gcgatgcagc tcggcctggc cgacgaaggc
     1501 agctggcgcg gcgagctgaa ccaccaccgc cgcaacggcg agccctgcgt gctgcagctc
     1561 accgtcacgg ccgtgcgcaa cgcgaacggc agcgtgcgca accacgtgct cgcgatcgcc
     1621 gacgtcacgc acgcgcgcgc gcagttcgag caattgcagc gccaggcgca tttcgacgaa
     1681 ctcacgcggc tgccgaatcg ggtccggctc gcccggatgc tgcactcggc gatgcagacg
     1741 agccgccgcg aaggctcgct gctgacggtt tgctacctcg acctcgatca cttcaagccc
     1801 gtcaacgacc gtttcggtca cgaggccggc gatcgcctgc tggtcgagct cgccggccgc
     1861 atgaagcgct cgctgcgctc gtgggcgggt ggcgacgacg tggtcgcgcg catcggcggc
     1921 gacgagttcg tgctgctgct gcgcaccgcc acgctggagg agagccgcca cgcagtcgag
     1981 cgcgtgctga accaggtctc ccagccctat ggcctgggcg tcggcgccgg cccggtgatg
     2041 gtgaccgcga gcatcggcgc gaccgtgttc ccgctcgacg gcgccgacgc cgaaaccctg
     2101 ctgcgccatg ccgatcacgc gatgtacggg gccaagcagg ccggccgcaa cggctatctg
     2161 ttcttcgatg ccgagcacga ccgccgcgcc gaggcgcgct tcgtcgcgct cggccgggtc
     2221 caggaggcgc tcgacgcgaa cgagttccgc ctgtacttcc agcccaaggt cgacatgcgc
     2281 aatgccaagg tgctcggcgt cgaggcgctg ctgcgctgga agcaccccga gcagggcgtg
     2341 atctcgccgg cgcagttcct gccgctgatc gagaacaccg ggctggcgat cagcgtcggc
     2401 aactgggtgc tgcagcaggg catcgaccag ctcgcgcgct ggctgcggct cggcctggac
     2461 atcaccgtca gcatcaacgt ctcggcgcgc cacctgcagg agccgctgtt cgcgcagcac
     2521 ctggccgggc tgctgtcgcg ccaccaggcg ccggtggcgc accggctgat catcgaggtg
     2581 ctcgagaccg ccgcgctcgc cgacgtggac tacacctgcg agctgatgga agagtgccgc
     2641 gcgctcggcg tgcgcttcgc gctcgacgac ttcggcaccg gctactccac cttcacgtac
     2701 ctgaagcggc tgcccatcga catgctgaag atcgaccgct cgttcgtgat caacatgctc
     2761 ggcgaccggc aggatctggc gatcgtcgag ggcgtgatcg gcctgtcgca cactttcggc
     2821 tgctcggtgg tggccgaagg cgtcgagacg cgcgcgcagg ccgagcgcct gatcgcgatc
     2881 ggctgcgcca tcggccaggg caacggcatc gccgcc
//
LOCUS       DASRDG010000211         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1181950, whole genome shotgun sequence.
ACCESSION   DASRDG010000211 DASRDG010000000
VERSION     DASRDG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181950"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /gene="tssG"
                     /locus_tag="VFA35_03475"
     CDS             complement(<1..769)
                     /gene="tssG"
                     /locus_tag="VFA35_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HZT55261.1"
                     /translation="MSAQTTIVERSAGDSGDSVRAQRVFDEIESAPHRYDFFAVLRIV
                     ESLHRARPRLGRALRPRDEPLRLGQDPEMDFAPAAVSSFEQGPRGVRRLGQRFFGLFG
                     PMGPLPLHLTEYARERQRNHGDATLARFADQFHHRALLLFYRAWAQAQPTVHLDRRAD
                     DGFSRWLGSLAGIGDAAFTGRDSLSDDAKRHRVALLAHGAKTAEGLTKLLRGWFGTAV
                     RLESHVGHWLAIRPEDRTRLLPPTQPGLRNVLGISAVA"
     gene            complement(766..>2161)
                     /gene="tssF"
                     /locus_tag="VFA35_03480"
     CDS             complement(766..>2161)
                     /gene="tssF"
                     /locus_tag="VFA35_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HZT55262.1"
                     /translation="HPPARARRVERAAIPLSQLEFHISAPGEAGYRLHELIGGAAIGS
                     LVWSPHMRGVAALAQWRDAAGSVALSGHGNRQALLPETLRGFSAYRLLQELAALPQRF
                     LFFELRQLAERIVRVPAAEVDLIILFSRAEPTLEALVDAASVSLFCTPAINLFAKRLD
                     RIAVDAGAHEFHAVPDRTRPMDFEVHSIESVTGYGTGQVSTQEFWPLYAAFHTEPAQH
                     PGYFSLRRVPRLLSQRQTQQGTRSAYIGSETFLSLVQPDGEPYLEDLRQLAVSALVTN
                     RDLPILLGGSPNGEPNQGQMAWALDGVGVVQAVECLRGPTRPTARAAWGPHGWSLINQ
                     LTLNHLSIDGEDPRRAAAALRSVLALYGPDQDMNWSKQVEGIVAVRAVPVTRRLPQGG
                     PLTFGSGVQVEVEVDELGFQGASAYLMGSVLEHFFARQAAINTFTETVLRSSTRGVIG
                     HWPPRVGEQPLLSP"
BASE COUNT          348 a          757 c          750 g          306 t
ORIGIN      
        1 cggccaccgc gctgatgccc agcacgttgc gcagacccgg ctgcgtgggc ggcagcagcc
       61 gcgtgcggtc ttcggggcgt atcgcgagcc agtgaccgac gtgcgactcg agccgcacgg
      121 cggtgccgaa ccacccccgc aacagcttgg tcagcccttc ggcggtcttc gcaccgtggg
      181 caagcagcgc cacgcggtga cgcttggcgt cgtcgctcag gctgtcgcgg ccggtgaagg
      241 cggcgtcgcc gatgcccgcc agcgacccaa gccagcgcga gaacccgtcg tcggcacggc
      301 ggtccaggtg caccgtcggc tgcgcctgcg cccaggcgcg gtagaacagc agcagcgcgc
      361 ggtggtggaa ctggtctgca aagcgcgcca gcgtcgcatc gccatggttg cgctggcgct
      421 cgcgcgcgta ctcggtcagg tgcagcggca gcggccccat cggcccgaac aggccgaaga
      481 agcgctggcc caggcggcgc accccacgcg ggccttgttc aaacgacgac acggccgccg
      541 gtgcgaagtc catctccggg tcctggccca ggcgcagcgg ctcgtcgcgc ggccgcagcg
      601 ctcggcccag ccgcgggcgt gcgcgatgga gtgattcgac gatgcgcaac acggcaaaga
      661 agtcgtagcg gtgaggtgcg gattcgatct cgtcgaacac acgttgcgcg cgaacgctgt
      721 cgccgctgtc gccagcgctt ctctcgacga tcgtcgtctg cgcgctcatg gcgacagcaa
      781 gggctgctcg cccacccgtg gcggccagtg cccgatcacg ccgcgcgtcg aagagcgcag
      841 cacggtctcg gtgaaggtgt tgattgccgc ctgtcgcgcg aagaagtgtt cgagcacgct
      901 gcccatcaga taggcgctag cgccctgaaa ccccagttcg tcgacttcga cttcgacctg
      961 cacgccgctg ccgaatgtca gcggcccgcc ttgcggtagc cgccgcgtca ccggcacggc
     1021 acgcaccgcg acgatgccct cgacctgctt gctccagttc atgtcctggt caggaccgta
     1081 gagcgcgagc acgctgcgca gcgcggccgc ggctcggcgc gggtcctcgc cgtcgatcga
     1141 caggtggttc agcgtgagct gattgatcag cgaccagccg tgcgggcccc acgcggcgcg
     1201 tgcggtcggc cgcgtcgggc cacgcaggca ttcgaccgcc tgcaccacgc ccacgccgtc
     1261 gagggcccac gccatctggc cctggttggg ctcgccgttg ggcgagccgc cgagcagaat
     1321 cggcaagtcg cgattggtga ccagcgccga cacagcgagt tggcgcaggt cttcgaggta
     1381 cggctcgccg tcgggttgga ccagcgacaa gaacgtttcg ctgccgatat aggccgagcg
     1441 cgtgccctgc tgcgtctggc gctgcgacag caatcgcggc acgcgcctga ggctgaagta
     1501 ccccggatgc tgtgccggct cggtgtgaaa agcggcatac agcggccaga actcctgcgt
     1561 gctgacctga ccggtgccgt agccggtgac cgactcgatg ctgtgcacct cgaagtccat
     1621 cggccgggta cggtccggca ccgcatgaaa ctcgtgcgcg ccggcgtcca ccgcgatgcg
     1681 atcgagccgc ttggcaaaca ggttgatggc gggcgtgcag aacaacgaga cgctcgcagc
     1741 atcgaccagc gcctcgaggg tgggctcggc gcgcgagaac aggatgatca gatcgacctc
     1801 ggcggcaggc acgcgcacga tgcgctcggc cagctgccgt agctcgaaga acaggaagcg
     1861 ctgcggcagc gcggccaact cctgtagcag ccggtaggcc gaaaagccgc gcagggtttc
     1921 cggcagcagc gcctgccggt tcccgtggcc ggacaaggcc accgagccag ccgcatcgcg
     1981 ccactgcgca agcgccgcaa cgccgcgcat gtgcggcgac cagaccagac tgccgatcgc
     2041 cgcgccaccg atcagctcgt gcaggcgata gcccgcctcc cccggtgcgc tgatgtggaa
     2101 ctcgagttgc gacagcggaa ttgctgcgcg ttcaacccgc cgtgcacgcg caggcggatg
     2161 c
//
LOCUS       DASRDG010000212         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_957452, whole genome shotgun sequence.
ACCESSION   DASRDG010000212 DASRDG010000000
VERSION     DASRDG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957452"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFA35_03485"
     CDS             complement(<1..1162)
                     /locus_tag="VFA35_03485"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HZT55263.1"
                     /translation="MTRPFIAPGATLGVMGGGQLARMFVHAAQQMGYATAVLDADPDS
                     PAGRIAHHHIRADYLDEAGLARLAELSAAITTEFENVPARALAMLAAQRPVAPGAAAV
                     AICQDRAAEKAHFGRSAVACAPYTVIESAAQLAAVPGDLLPGILKTARLGYDGKGQLR
                     VANAAELASGWAALGGVTCVLEKMLPLAHELSVIVARGADGQVVNLPVQQNLHRDGIL
                     AITQVPAATVGAEAQQRAVESARQIAAELGYVGVLCVEFFVLADGALVANEMAPRPHN
                     SGHYSIDACDVSQFDLQVRALTGAPLVAPRLHSPCVMLNLLGDLWFDAAGAPLDAGPD
                     WAGVLALPGVHLHLYGKAQARVGRKMGHLTVTAATAALADRVAREAAARLGIE"
     gene            complement(1159..1623)
                     /gene="purE"
                     /locus_tag="VFA35_03490"
     CDS             complement(1159..1623)
                     /gene="purE"
                     /locus_tag="VFA35_03490"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZT55264.1"
                     /translation="MGSSSDWDTLRAATEILAEFGIPHEARVVSAHRMPDEMFAYAEQ
                     AAARGLKAIIAGAGGAAHLPGMLAAKTTVPVLGVPVASRHLQGVDSLHSIVQMPKGIP
                     VATFAIGAAGAANAALFAVAMLANDDPALRATLDAYRARQTAAARAMTADLK"
     gene            1761..2705
                     /locus_tag="VFA35_03495"
     CDS             1761..2705
                     /locus_tag="VFA35_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT55265.1"
                     /translation="MPDISLQNLETDLIQASTQQPVLLDIWAPWCGPCKALGPVLEKL
                     EADYAGRFVLAKLNSEDQPEIAAQLSQMFGVRSIPFCVLFKDGQPVDGFVGALPETEI
                     RAFLDKHVPSADALAAADDIDEANALLADGDTESALEKLQQAVAVNPGNDSARNDYLR
                     ALLGAGRIDEARRAYEPVASKALLDARLAAAGHWLAACERSAQARSPEQLAAAIGADK
                     RDFEARFELAQTHFAAQRFTQAMDELLEIVMRDKSWRDELARKTYVAILELMNKPAPA
                     KDEAGAQKGKLELAGKAAVVPSDPVVDAYRRKLSMALF"
     gene            complement(2723..>3184)
                     /locus_tag="VFA35_03500"
     CDS             complement(2723..>3184)
                     /locus_tag="VFA35_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55266.1"
                     /translation="TGAESTARFDDMLGVTSFSELSPMIHAMLFTVLGETADALQLYE
                     AHLDAAMQQGFDRMEGLLRADRAWCRVQAGQQEGARSDAELADQCLQRPGGWSDRAIG
                     HSRLVRVYQALGDGDAARRQAELAASAWAGHRALQAELVEALDRELGRIAS"
BASE COUNT          492 a         1139 c         1120 g          433 t
ORIGIN      
        1 cttcgatgcc gagccgtgcg gcggcctcgc gcgcaacgcg gtcggccagc gccgcggtgg
       61 cggccgtcac ggtcaggtgg cccatcttgc gcccgacgcg agcctgcgcc ttgccgtaca
      121 ggtgcaagtg cacgccgggc agcgcgagca cgccagccca gtccgggccg gcatcaagcg
      181 gcgcgccggc agcgtcgaac cacaggtcgc cgagcaggtt cagcatcacg catggcgagt
      241 gcaggcgcgg cgcgaccagc ggcgcgccgg tcagcgcgcg cacctgcagg tcgaactgcg
      301 agacatcgca ggcgtcgatg ctgtagtggc cggagttgtg cgggcgcggc gccatctcgt
      361 tggcgaccag cgcgccgtcg gcgagcacga agaactcgac gcagagcacg ccgacatagc
      421 cgagctcggc agcgatctgg cgcgcgctct cgaccgcgcg ctgttgcgcc tcggcgccga
      481 ccgtggcggc cggcacctgc gtgatcgcca ggatgccgtc gcgatgcagg ttctgctgca
      541 ccggcaggtt gacgacctgg ccgtcggcgc cgcgcgcgac gatcacgctg agctcgtgcg
      601 ccagcggcag catcttctcg agcacgcagg tgacgccgcc gagcgcggcc cagcccgacg
      661 ccagctcggc agcattggcg acgcgcagct ggcccttgcc gtcgtagccg agccgcgcgg
      721 tcttcaggat gccgggcagc agatcgccgg gcaccgcggc gagctgggcg gccgactcga
      781 tgaccgtgta cggcgcgcag gcgacagcgc tgcggccgaa atgcgccttc tcggcggcgc
      841 ggtcctggca gatcgcgacc gcggccgcgc cgggcgcgac cgggcgctgc gccgccagca
      901 tcgcgagcgc acgcgcgggc acgttctcga actcggtcgt gatggccgca ctgagctcgg
      961 cgagccgcgc gaggccggcc tcgtcgaggt agtcggcgcg gatgtggtgg tgcgcgatcc
     1021 gtccggccgg gctgtcgggg tcggcgtcga gcaccgcggt cgcgtagccc atctgctgcg
     1081 cggcatgcac gaacatgcgg gcgagctggc cgccgcccat gactcccagg gtcgccccgg
     1141 gggcgatgaa cggccgggtc atttcagatc ggcggtcatc gcacgcgccg cggcggtctg
     1201 acgggcgcga taggcatcga gcgtcgcgcg caaggccggg tcgtcgttgg ccagcatcgc
     1261 gacggcgaac agcgccgcgt tggcggcgcc ggccgcaccg atcgcgaagg tcgcgaccgg
     1321 gatgcccttg ggcatctgca cgatcgaatg gagcgaatcg acgccctgca gatgacggct
     1381 ggcgaccggc acaccgagca ccggcacggt cgttttcgcg gccagcatgc cgggcaggtg
     1441 cgcggcgccg ccggcgccgg cgatgatcgc cttcagaccg cgcgccgcgg cctgctcggc
     1501 atacgcgaac atctcgtcgg gcatgcgatg ggccgagacg acgcgcgctt catgggggat
     1561 gccgaactcg gcgagaatct cggtggctgc gcgcagggtg tcccagtcgc tgctggaacc
     1621 catcaccacg ccgaccacca cgggggcgct gctcaaagct cgctccaggg tgctgatgaa
     1681 cccgaattgt aggcggggcc tgcgcagcgc ttcgcccggc cgctctcgtc accttcaccc
     1741 gcgatgcggg aatccggacc atgccagaca tctcactgca aaacctcgaa accgacctga
     1801 tccaggcctc gacgcagcag cccgtgctgc tcgacatctg ggcaccgtgg tgcggcccgt
     1861 gcaaggcgct cgggcccgtg ctggagaagc tcgaagccga ctacgcgggc cgcttcgtgc
     1921 tggccaagct caactcggag gaccaacccg agatcgcagc ccaactctcg cagatgttcg
     1981 gcgtgcgcag catcccgttc tgcgtgctgt tcaaggacgg ccagccggtc gacggcttcg
     2041 tcggcgcgct gcccgaaacc gagatccgcg cctttctcga caagcacgtg cccagcgccg
     2101 acgcgctcgc cgctgcggat gacatcgacg aagcgaacgc gctgctggcc gacggcgaca
     2161 ccgagagcgc gctcgagaag ctgcagcagg cggtcgccgt caaccccggc aacgacagcg
     2221 cgcgcaacga ctacctgcgc gcactgctcg gcgccggccg catcgacgaa gcgcgccgcg
     2281 cctacgaacc cgtggcctcg aaagcgctgc tcgacgcgcg cctggccgcg gccggccatt
     2341 ggctggcggc ctgcgagcgc agcgcccagg cgcgctcgcc cgagcagctc gcggccgcga
     2401 tcggcgccga caaacgcgac ttcgaggccc gcttcgagct ggcgcagacc cacttcgcgg
     2461 cgcagcgttt cacgcaggcg atggacgagc tgctcgagat cgtgatgcgc gacaagagct
     2521 ggcgcgacga actggcgcgc aagacctacg tcgccattct cgagctgatg aacaagccgg
     2581 cgccggcgaa ggacgaggcc ggagcgcaaa agggcaagct cgaactggcg ggcaaggccg
     2641 cggtcgtgcc gtccgatccg gtggtcgacg cgtaccgccg gaagctgagc atggcgctgt
     2701 tctgacaggc cggatcgcgc gctcagctcg cgatccggcc gagctcccga tccagcgctt
     2761 ccaccagctc cgcctgcagc gctcgatgcc cggcccaggc gcttgctgcg agttccgcct
     2821 ggcgccgcgc cgcgtcgccg tcgcccagtg cctggtagac ccggaccagc cggctgtggc
     2881 cgatcgcccg gtcggaccag ccgcccgggc gctgcaggca ttgatcggcc aactcggcat
     2941 cggaacgtgc tccttcttgc tggccggctt gcacgcggca ccaagcccga tcggccctca
     3001 acaggccttc catccggtca aatccctgtt gcattgcggc atcaaggtgc gcttcataca
     3061 gttggagcgc atcggcggtc tcgccaagca cggtgaacaa catcgcgtgg atcatcggcg
     3121 acagctcgct gaaactggtt acccccaaca tgtcgtcgaa gcgggctgta gattcagcgc
     3181 cggt
//
LOCUS       DASRDG010000213         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_602394, whole genome shotgun sequence.
ACCESSION   DASRDG010000213 DASRDG010000000
VERSION     DASRDG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602394"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFA35_03505"
     CDS             complement(<1..566)
                     /locus_tag="VFA35_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZT55267.1"
                     /translation="MKPEPTARAEHHFSWHLARRAERMNPSVIREILKLTERPGIISL
                     AGGLPSPDSFPVEAMREATARVLRDNPREALQYAASEGFAPLREWVAAHMRAQGMAVD
                     AAQVLITTGSQQGLDLVGKVLIDAGSTLAVESPTYLGALQAFAPYEPEVLTVPCDDDG
                     PLHESLGVARGARFLYVIPNFQNPSGRC"
     gene            complement(566..1492)
                     /locus_tag="VFA35_03510"
     CDS             complement(566..1492)
                     /locus_tag="VFA35_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZT55268.1"
                     /translation="METNTLRLKRDGTRGLWLGLLGVVIFAMTLPMTRLAVGPATDPQ
                     LPPLFVTAGRAACAGLLSVLYLRFTNAPHVQRRHLPALFTSALGTVVGFPLFLSLALR
                     QVDAMHAAVVTGLMPLATAIGAAVFLRQRPSNGFWACAALGCAMVLAFAAYRGSGHLS
                     PADGLLLIAVGFTAVGYVAGARVAAELPAEQVICWVLVLSLPLTLPLAIATWPAAPVR
                     ASAWGGFAYVTLFSMWLGFFAWYRGLALGGTVRVSQVQLVQPFLALLFAVPVLGETLD
                     ALTVVFALAVIAVVFVGKKMPAGAPALQPRNP"
     gene            1585..>2081
                     /locus_tag="VFA35_03515"
     CDS             1585..>2081
                     /locus_tag="VFA35_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZT55269.1"
                     /translation="MPATTSPLAAAPLSRAADTTLTEQLASRFAERIRQRLLAPGARL
                     PSVRECARRHGVSPHTVVAAYDQLLALGLVEARRQRGFFVRESAAPRAAAGPGHAAHA
                     AERVPAHPLPISATALIRGMFQPPGARPMPGLGTLPADWLDLPLLAGALRKVSSGEQL
                     GGVTLR"
BASE COUNT          320 a          766 c          754 g          241 t
ORIGIN      
        1 atgcagcgcc cgctcgggtt ctggaagttc gggatgacgt acaagaaccg cgcgcccctg
       61 gccacaccga gcgactcgtg tagcgggccg tcgtcgtcgc acggcacggt gagcacctcc
      121 ggctcgtagg gcgcgaaagc ctgcagcgcg cccaggtagg tgggcgattc gaccgcgagc
      181 gtgctgcccg cgtcgatcag caccttgccg acgaggtcga ggccctgctg cgagccggtg
      241 gtgatcagca cctgcgcggc gtcgaccgcc atgccttgcg cgcgcatgtg cgcggcgacc
      301 cactcgcgca acggcgcgaa gccttcgctc gccgcgtact gcagcgcctc gcgcgggttg
      361 tcgcgcagca cgcgggcggt cgcctcgcgc atcgcctcga ccgggaagct gtcgggcgac
      421 ggtagcccgc cggccagcga gatgatgccg gggcgctcgg tgagcttcag gatctcgcgg
      481 atcaccgacg ggttcatgcg ctcggcgcgg cgcgccagat gccacgagaa atggtgctcg
      541 gcccgcgcgg tgggttcggg tttcatcacg gattccgggg ttggagggca ggcgcgccgg
      601 ccggcatctt cttgccgacg aacacgaccg cgatcacggc gagcgcgaag accacggtga
      661 gcgcgtcgag cgtctcgccg agcaccggca ccgcgaacag cagcgcgagg aacggctgca
      721 ccagttgcac ctggctgacg cgcaccgtgc cgccaagcgc gaggccgcgg taccaggcga
      781 agaagccgag ccacatcgag aacagcgtga cgtaggcgaa gccgccccac gcgctcgcgc
      841 gcaccggcgc cgccggccag gtggcgatcg cgagcggcaa agtcagcggc aggctgagca
      901 ccagcaccca gcagatcacc tgctcggccg gcagttcggc tgcgacgcgc gcgccggcga
      961 cgtagccgac cgcggtgaag cccaccgcga tcagcagcag gccgtcggcc ggcgacaggt
     1021 gcccgctgcc gcggtaggcc gcgaacgcca gcaccatcgc gcagcccagc gccgcgcaag
     1081 cccagaagcc gttcgacggc cgctggcgca ggaacactgc cgcgccgatc gcggtcgcca
     1141 gcggcatcag cccggtgacg accgcggcgt gcatcgcgtc gacctggcgc aacgccaggc
     1201 tcaggaacag cgggaagccg acgacggtgc cgagcgcgga agtgaacagc gccggcaagt
     1261 ggcgacgctg cacgtgcggc gcgttggtga accgcaggta cagcacgctg agcagcccgg
     1321 cgcaagcggc gcggccggcg gtgacgaaca gcggcggcag ttgcgggtcg gtggcggggc
     1381 cgaccgccag gcgcgtcatc ggcaaggtca tcgcgaagat cacgacgccg agcaggccga
     1441 gccacagacc gcgcgtgccg tcgcgcttca gccgaagggt gttcgtctcc atgcgatcag
     1501 tctatgccca cacaccagca cagagacagt acacttcgcg gcacaatatc aacctctgta
     1561 ctggctctat cgacgacacg cgagatgccc gccacgacct ccccgctcgc cgccgccccg
     1621 ctctcgcgcg cggccgacac cacgctgacc gagcagctcg cgagccggtt cgcggagcgc
     1681 atccgccagc gcctgctcgc gccgggcgcg cggctgccct cggtgcgcga gtgcgcgcgg
     1741 cgccatgggg tgagcccgca cacggtcgtc gcggcctacg accagctgct cgcgctcggc
     1801 ctggtcgagg cgcgccggca gcgcggcttc ttcgtgcgcg agagcgcggc gccgcgcgcc
     1861 gccgccggac cggggcacgc cgcccacgcc gccgaacgcg tgccggcgca cccgttgccg
     1921 atcagcgcca ccgcgctgat ccgcggcatg ttccagccgc ccggcgcgcg gccgatgccg
     1981 gggctgggca cgctgccggc cgactggctc gacctgccgc tgctggccgg cgcgctgcgc
     2041 aaggtgtcga gcggcgagca gctcggcggc gtcacgctgc g
//
LOCUS       DASRDG010000214         5906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1293054, whole genome shotgun sequence.
ACCESSION   DASRDG010000214 DASRDG010000000
VERSION     DASRDG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293054"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFA35_03520"
     CDS             <1..911
                     /locus_tag="VFA35_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZT55270.1"
                     /translation="PGDIDGWRAAIRPNTKLLFGETLGNPGLDVLDIPRVAELAHAHG
                     LPLLVDSTFTTPWLMQPFDHGADLLFHSATKFLSGHGTVIGGVLVDSGRFDWHAAHAR
                     HGRFGELAQPYDGFHGMVFSEESTVGAFLLRARREGLRDFGACMSPHTAWLILQGIET
                     LPLRMARHIENTRKVVAFLAAHPMVAGVAYPELESHLSHALARQLLPRGCGSVFSFDL
                     RGSRKQGVAFIEALKIFSHLANVGDCRSLVIHPASTTHFRMDDAALAQAGITAGTIRL
                     SIGLEDADDLIDDLRRALKIAEKAGA"
     gene            911..1723
                     /locus_tag="VFA35_03525"
     CDS             911..1723
                     /locus_tag="VFA35_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT55271.1"
                     /translation="MELLVEGPQGPAAAYAYTGGKAFDAALPCVVFVHGALHDHSDWT
                     LLARWCAHHGHGVLAVDLPGHGRSGGAPLASVEALAEWLLALLDAAGVERATLVGHSM
                     GSLIALEAAARAPQRVRALVMIGTAYPMKVSDALLSTAREAPLRAIDMVNAFSHSGIA
                     AKPSFPGPGTWLHGADRALMRRLQADQTALNLFHHDFSVCDRYSGGLQAAPGVACPVT
                     FILGARDQMTLAKHTGEFAQALRAQVRRVNAGHALMSEAPDAVLAALRCALA"
     gene            1743..2066
                     /locus_tag="VFA35_03530"
     CDS             1743..2066
                     /locus_tag="VFA35_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HZT55272.1"
                     /translation="MNTASVDLKAIKSFAEFYPLYLQEHRNPTCRRLHFIGSSLCLVC
                     LALLAVTGQPGFVLGGLLCGYGFAWVGHFVFEKNKPASFKRPLYSFMGDWLMYADIWR
                     GKIRF"
     gene            2069..3349
                     /locus_tag="VFA35_03535"
     CDS             2069..3349
                     /locus_tag="VFA35_03535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZT55273.1"
                     /translation="MVAAAEPRLTHTERLIAAIDLPIGRWDRAARLVFCNDPYLAWAG
                     RPREQLLGRTLQELFGQPAWAAAAAAFTQAFTGRTASYQRRLAHGPACGRWARIQVCP
                     DADAAGTVDTVFTLATDIHDDMVARESLLAAQTRLDRFADNIPYPLTYVDRGFVLHFV
                     NKAYTELVGQRRDELIGRHIGEVRGARRWAEHEPYFQRALSGESVQYTRLASTLPQGS
                     RWLRTSYVPDFDADGQVVGLYTVTVDVHELAVTQEKLKRRAEHDALTDVLSRRTMMDR
                     VELALADAACQPVALYFVDLDGFKAVNDRLGHREGDALLVAVGAALQQAVRAEDAVGR
                     FGGDEFLVMARVRDAAGAHALALHMLAAVQAVAGGRPLAGAVSASIGYALAPGDAHHP
                     LNLLQLADDAMYAAKRSGKNRAMHCAATPFEVAR"
     gene            3352..4620
                     /locus_tag="VFA35_03540"
     CDS             3352..4620
                     /locus_tag="VFA35_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT55274.1"
                     /translation="MPAQPRARRGWPARLLLPAGADADAWRLLAARGLRALADGTMAV
                     LLPAYLLALGLGTVEVGVVATTTLLGSALATLAVGTWGHRIAHARLLRAAAVVMAATG
                     LGFAGFSSLWPLLVVAFLGTLNPSAGDVSVFLPLEHAKLAAAAAGASRTALFARYSVI
                     GAVCAALGALATGLPDLLVRAFAWPRLDAMRAMFVLYAVIGGLVWLLYRQPPAQQVEA
                     RAAHAAPPAPPAPLGPSRGIVIRLAALFSVDAFAGGLVVNSLLSLWLLQRFGLSLAQA
                     GAFFFWTGLLSAASLLAAPGVARRFGLLNTMVFTHIPANVCLVLAALAPNLPLALGLL
                     FVRSALSQMDVPTRSAYVMAVVTPAERPAAASFTAVPRSLAAALSPALSGALFAAGWF
                     AAPLLACGVIKIAYDLALWRACLRDKLGEH"
     gene            complement(4634..5869)
                     /locus_tag="VFA35_03545"
     CDS             complement(4634..5869)
                     /locus_tag="VFA35_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HZT55275.1"
                     /translation="MKLTTTIKSAVQEQLPDLVQTLQTWPWLDTLRTLRARFREDRLG
                     LTASSLTFTTLISLVPLLTVMLAVFSAFPMFASFQDSLQKYFLQTLVPQDIASQVLKA
                     LTTFAAKAKGVGSVGLVLLVFAALALMLTIDRTLNGIWRVRTARPIAQRVLVYWAAAT
                     LGPLVLGVSLSLTSYALSASQGLVGGMPGGVSLLLEAIEFLLLVAAFAALFHYVPNTH
                     VRWRHALGGAAFSAAGYELAKKLLGWYMQTVPTYTVIYGALAAVPIFLIWVYLAWVIV
                     LLGAVVAAYAPSLQMRVKRWPDGPGARLQLALAVIRELARAHASGERGASGEQLAAAL
                     RTDPLQIEPILETLQQFDWVGRLDEPRDPRFVLLCDPARTPAQPLLAQLLLAPSPASH
                     GLWQRAGFDTMTLQELLAA"
BASE COUNT          826 a         2125 c         2072 g          883 t
ORIGIN      
        1 agccgggcga catcgacggc tggcgcgccg cgatccgacc gaacaccaag ctgctgttcg
       61 gcgaaacctt gggcaacccc gggctggacg tgctcgacat cccccgggtc gcggaactcg
      121 cccacgcgca cggcctgccg ctgctggtcg attcgacctt cacgacgccg tggctgatgc
      181 agcctttcga tcatggcgcc gacctgctgt ttcattcggc gacgaagttc ttgtcgggcc
      241 acggcaccgt gatcggcggc gtgctggtcg acagcggccg gttcgactgg cacgccgcac
      301 acgcgcggca tggccgcttc ggcgaactcg cgcagcccta cgacggcttt cacggcatgg
      361 tgttcagcga ggagagcacg gtcggcgcct tcctgctgcg cgcgcgccgc gaaggcctgc
      421 gcgacttcgg cgcctgcatg agcccgcaca ccgcgtggtt gatcctgcag ggcatcgaaa
      481 ccttgccgct gcgcatggcg cggcacatcg agaacacgcg caaggtggtg gcgtttctcg
      541 ccgcgcaccc gatggtcgcg ggcgtcgcct accccgagct cgaatcgcac ctcagccatg
      601 cactcgcgag gcagctgctg ccgcgcggct gcggctcggt cttcagcttc gacctgcgtg
      661 gctcgcgcaa gcaaggggtc gcattcatcg aggctttgaa gatcttctcg cacctcgcca
      721 acgtcggcga ctgccgctcg ctggtgatcc acccggcgtc gaccacgcac ttccgcatgg
      781 acgacgcggc gctcgcgcag gccggcatca ccgccgggac gatccgattg tcgatcgggc
      841 tcgaagacgc cgacgacctg attgacgacc tgaggcgcgc gctgaagatc gccgagaaag
      901 caggcgcgtg atggaactgc tcgtcgaagg gccgcaagga ccggctgcgg cctacgccta
      961 caccggtggc aaggcgttcg atgcggccct gccctgcgtc gtttttgtcc acggggcgct
     1021 gcacgaccac agcgactgga cgctgctcgc gcgctggtgc gcgcaccacg ggcacggcgt
     1081 gctggccgtc gacctgccag ggcacggccg cagcggcggc gcgccgctgg cgagcgtcga
     1141 ggcgctggcc gagtggctgc tcgcattgct cgatgccgcc ggggtcgaac gcgccacgct
     1201 ggtcggccac agcatgggct cgctgatcgc gctcgaagcc gcggcacgcg caccgcaacg
     1261 cgtgcgtgcg ctggtgatga tcggcaccgc ctacccgatg aaggtctcgg acgcgctgct
     1321 gagcacggcg cgcgaggcgc cactgcgggc gatcgacatg gtcaacgcgt tctcgcacag
     1381 cggcatcgcc gcgaagccct cgttccccgg gccgggcacc tggctgcacg gggccgaccg
     1441 ggcgctgatg cggcggctcc aggccgatca gacggcgctc aacctgttcc accacgactt
     1501 cagcgtctgc gaccgctaca gcggcggcct gcaggcggcg ccaggtgtcg cctgcccggt
     1561 caccttcatc ctcggcgcgc gcgaccaaat gaccttggcc aagcacaccg gcgagttcgc
     1621 gcaggcgctg cgggcgcaag tgcggcgtgt caacgcgggc cacgcgctga tgagcgaagc
     1681 gcccgatgcg gtgctggccg cgctgcgctg cgcgctggcc tgacccacga aggagacgaa
     1741 tcatgaacac cgccagcgtc gacctgaagg cgatcaagtc gttcgccgag ttctacccgc
     1801 tgtacctgca ggagcaccgc aacccgacct gccggcgcct gcacttcatc ggctccagcc
     1861 tgtgtctggt ctgcctggcg ctgctggcgg tcaccggcca gcccggcttc gtgctgggcg
     1921 ggctgctgtg cggctacggc ttcgcctggg tcggccactt cgtgttcgag aagaacaagc
     1981 cggcgtcgtt caagcgcccg ctgtacagct tcatgggcga ctggctgatg tacgccgaca
     2041 tctggcgcgg aaaaatccgg ttctgatcat ggttgccgcc gccgagcccc gcctcacgca
     2101 caccgagcgc ctcatcgcgg cgatcgatct gccgatcggc cgctgggatc gcgccgcccg
     2161 cctcgtcttc tgcaacgacc cctacctcgc ctgggccggc cggccgcgcg agcagctgct
     2221 ggggcgcacg ctgcaggagc tgttcggcca gcccgcctgg gccgccgcag cggccgcgtt
     2281 cacgcaggcc ttcaccggcc gcaccgccag ctaccagcgc cggctggcgc acgggccggc
     2341 atgcggccgc tgggcacgca tccaggtctg ccccgacgcc gatgccgccg gcacggttga
     2401 caccgtgttc acgctcgcga ccgacatcca cgacgacatg gtggcgcgcg agagcctgct
     2461 cgccgcgcag acccgcctcg accgcttcgc cgacaacatc ccgtacccgc tgacctatgt
     2521 cgaccgcggc ttcgtgctgc acttcgtcaa caaggcctac accgaactgg tcggccagcg
     2581 ccgcgacgag ttgatcggcc ggcacatcgg cgaggtgcgc ggcgcgcgcc gctgggccga
     2641 acacgagccc tactttcagc gtgcgctgag cggcgagtcg gtgcagtaca cgcggctggc
     2701 gagcacgctg ccgcagggct cgcgctggct gcgaaccagc tacgtgcccg acttcgacgc
     2761 cgacggccag gtcgtcggcc tgtacacggt cacggtcgac gtgcacgaac tcgcggtgac
     2821 gcaggaaaag ctgaagcgcc gtgccgagca cgacgcgctg accgacgtgc tcagccgccg
     2881 cacgatgatg gatcgcgtcg agctcgcgct ggccgatgcg gcatgccagc cggtcgcgct
     2941 ctacttcgtc gacctcgacg gcttcaaggc cgtgaacgac cgcctcggcc atcgcgaggg
     3001 cgacgcgctg ctggtcgcgg tcggcgcggc gctgcagcag gcggtgcgcg ccgaagacgc
     3061 ggtcggccgt ttcggcggcg acgagttcct ggtgatggcc agggtgcgcg acgccgcggg
     3121 cgcgcacgcg ctggcgctgc acatgctcgc cgcggtccag gccgtggccg gcggcaggcc
     3181 gctcgccggc gccgtcagcg cgagcatcgg ctacgcgctc gcgcccggcg atgcacacca
     3241 cccgctgaac ctgctgcagc tcgccgacga cgcgatgtac gccgccaagc gcagcggcaa
     3301 gaaccgcgcg atgcactgcg cggcaacgcc gttcgaggtg gcgcgatagg cttgccggcg
     3361 cagccccgag cgcgtcgcgg ctggcccgcc aggctgctgc tgcctgccgg ggccgatgcc
     3421 gatgcctggc ggctgctggc agcgcgcggc ctgcgcgcgc tggccgacgg caccatggcc
     3481 gtgctgctgc ccgcctacct gctggccctg gggctgggca cggtcgaggt cggcgtggtg
     3541 gcgacaacca cgctgctcgg ctcggccctg gccacgctgg cggtcggcac ctgggggcac
     3601 cgcatcgcac acgcacgcct gctgcgtgcc gccgccgtcg tgatggccgc caccggcctg
     3661 ggcttcgccg gcttcagctc gctgtggccg ctgctcgtcg tcgcattcct cggtacgttg
     3721 aacccgagtg cgggcgacgt cagcgtgttc ctgccgctgg agcatgcgaa gctcgctgcc
     3781 gcagcggccg gcgcgtcgcg caccgcactg ttcgcgcgct acagcgtgat cggggcggta
     3841 tgcgcggcgc tgggcgcgct ggcgacgggc ctgcccgacc tgctggtccg agcgttcgcg
     3901 tggccgcggc tggatgcgat gcgggcaatg ttcgtgctct acgccgtcat cggcggcctg
     3961 gtgtggttgc tgtaccggca gccaccagcg cagcaggtcg aggcacgagc cgcacatgcg
     4021 gcaccaccgg caccacccgc acccttgggg ccatcgcgcg gcatcgtgat ccggctcgcg
     4081 gcgctcttca gcgtcgatgc ctttgcgggc gggctggtgg tgaactcgct gctgtcgctg
     4141 tggctgctgc aacgcttcgg cttgtcgctg gcgcaggccg gcgcgttctt cttctggacc
     4201 ggcctgctga gcgcggcgtc gctgctcgcc gcgcccggcg tggcacgacg cttcggactg
     4261 ctgaacacga tggtgttcac ccacatcccg gccaatgtct gcctggtgct cgccgcgctc
     4321 gcgccgaatc ttccgctcgc gctcggcctg ctgttcgtgc gcagcgcgtt gtcgcaaatg
     4381 gatgtgccga cgcgttcggc ctatgtgatg gccgtggtca cgccggccga gcggccggcc
     4441 gcggccagct tcaccgcggt gccgcgcagc ctggctgcgg cactcagccc ggcgctcagc
     4501 ggggccttgt tcgcggcggg ttggttcgct gcgccgctgc tggcttgcgg cgtgatcaag
     4561 atcgcctacg acctcgcgct gtggcgcgcc tgcttgcgcg acaagctcgg cgagcactga
     4621 gcgttccggt gcctcacgcg gccagcagct cctgcagcgt catcgtgtcg aacccggcgc
     4681 gttgccacag cccgtgcgac gcgggggacg gcgcgagcag cagttgcgcg agcagcggct
     4741 gcgccggggt gcgcgccggg tcgcagagca gcacgaaacg cgggtcgcgc ggctcgtcga
     4801 ggcggccgac ccaatcgaac tgctgcagcg tctcgaggat cggctcgatc tgcagcgggt
     4861 cggtgcgcag cgccgctgcg agttgctcgc cgctcgcgcc gcgctcgccg ctcgcgtgcg
     4921 cccgcgccag ttcgcggatc accgcgagcg caagctggag ccgcgcgccg gggccgtcgg
     4981 gccagcgctt gacgcgcatc tgcaggctcg gcgcgtaggc cgcgacgacc gcgccgagca
     5041 gcacgatgac ccacgcgagg tagacccaga tcaggaagat cggcaccgcg gcgagcgcgc
     5101 cgtagatcac ggtgtaggtc ggtaccgtct gcatgtacca gcccagcagc ttcttggcga
     5161 gctcgtagcc ggccgcggag aacgcggcgc cgccgagcgc gtggcgccag cgcacgtgcg
     5221 tgttcggcac gtagtggaac aaggccgcga acgccgcgac cagcagcagg aactcgatcg
     5281 cttcgagcag cagcgagaca ccgcccggca tcccgccgac cagcccctgc gaggccgaca
     5341 gcgcgtacga cgtgaggctc aggctcaccc cgagcaccag cgggccgagc gtggccgcgg
     5401 cccagtagac cagcacgcgc tgcgcgatcg gccgcgcggt gcgcacgcgc cagatgccgt
     5461 tgagcgtgcg gtcgatcgtc agcatcagcg cgagcgcggc gaagaccagc agcacgaggc
     5521 cgacgctgcc gacgcccttg gccttggcgg cgaaggtggt cagcgccttc agcacctggc
     5581 tcgcgatgtc ctgtggcacc agcgtctgca ggaagtactt ctgcagcgag tcctggaagc
     5641 tcgcgaacat cggaaaggcc gagaacaccg cgagcatcac ggtcagcagc ggcaccagcg
     5701 agatcagcgt cgtgaaggtc aggctgctcg ccgtcaggcc gagccggtct tcgcgaaagc
     5761 gcgcgcgcag cgtgcgcagc gtgtcgagcc acggccaggt ctgcagggtc tggaccaggt
     5821 cgggcagctg ctcctgcaca gccgacttga tcgtggtcgt gagcttcatt gctggcatca
     5881 tagccagcga tgaatcacgc acccgc
//
LOCUS       DASRDG010000215         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1064952, whole genome shotgun sequence.
ACCESSION   DASRDG010000215 DASRDG010000000
VERSION     DASRDG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064952"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..564
                     /locus_tag="VFA35_03550"
     CDS             88..564
                     /locus_tag="VFA35_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZT55276.1"
                     /translation="MDIPLSLTVNGKSVTRTVDSRTLLVQFLRESLQLTGTHVGCDTA
                     QCGACTVHMNGRAIKSCSMLAAQVQGSQITTIEGLAQNGTMHPMQEAFRECHGLQCGF
                     CTPGMVMSAVQLLADNPQATEAEIREGLDGNICRCTGYQGIVKAIQFCQSGAKATA"
     gene            586..>2282
                     /locus_tag="VFA35_03555"
     CDS             586..>2282
                     /locus_tag="VFA35_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZT55277.1"
                     /translation="MSVPPLSPIGQSLKRREDQRFLTGAGQYTDDVVMHGQTYAAFLR
                     SPHAHARIKSIKLDAAKAAPGVVRIFTGQDLADAKVGGLPCGWLIHSKDGSPMKEPPH
                     PVLAQGKVRHVGDQVALVVAETLLQAKDACELIEVDYEELPAVIDITQADGHAGHVHD
                     DVPNNVCYDWGHGNKEAVDAAFAGAAHVSTLDIVNNRLIPNAMEPRAANASYTKHDES
                     YTLYVSNQNPHVERLLMCAFVLGLPESKVRVIAPDVGGGFGSKIFLYPEETALVWASK
                     HVGRPIKWTADRSEAFLTDAHGRDHVTKVELALDAKGMFLALRVKTIANLGAYLSTFA
                     SSVPTILYATLLAGQYKTPAIYAEVKAVFTNTAPVDAYRGAGRPEATYVVERIVETAA
                     RDLKLDPVAIRRQNFITEFPYATPVGLTYDTGNYEAHLAKAIAMADVADFAARRAASE
                     AKGKKRGLGYSCYIEACGLAPSNIAGALGARAGLFEAGEVRVHPTGTVTVFTGSHSHG
                     QGHETTFAQVVAAKLGIPVESIEIVHGDTGRVPFGMGTYGSRSLSVGGTAIVKAVDK"
BASE COUNT          404 a          802 c          759 g          317 t
ORIGIN      
        1 ctcgctccgc cgctctgaac aatgggtttt ccagggcgct tcccgtcaga gggtcggcgc
       61 cgagcgcaac gcagcaggag aaccggcgtg gacattcccc tttccctcac cgtcaacggc
      121 aagtccgtca ccaggacggt cgactcgcgc acgttgctcg tgcagttcct gcgcgagagc
      181 ctgcagctga ccggcaccca cgtcggctgc gacaccgcgc agtgcggtgc ctgcaccgtg
      241 cacatgaacg ggcgcgccat caagtcgtgc tcgatgctcg ccgcgcaagt gcagggcagc
      301 cagatcacga ccattgaggg gctggcccaa aacggaacaa tgcatccgat gcaggaggcc
      361 ttccgcgagt gccacggcct gcagtgcggc ttctgcacgc cgggcatggt gatgtcggcg
      421 gtgcagctgc tggccgacaa cccgcaggcg accgaggccg agatccgcga gggtctggac
      481 ggcaacatct gccgctgcac cggctatcag ggcatcgtca aggcgatcca gttctgccag
      541 tccggcgcca aggccacggc ctgaggagaa cccaccatga ccgacatgtc cgttcctccg
      601 ctgagcccga tcggccaatc gctgaagcgc cgtgaagacc agcgcttcct gaccggcgcg
      661 ggccagtaca ccgacgacgt cgtgatgcac ggccagacct acgccgcgtt cctgcgctcg
      721 ccgcacgcgc atgcgcgcat caagtcgatc aagctcgacg cggcgaaggc cgcgcccggc
      781 gtcgtgcgca tcttcaccgg ccaggacctg gccgacgcga aggtcggcgg cctgccgtgc
      841 ggctggctga tccacagcaa ggacggctcg ccgatgaagg agccgccgca cccggtgctg
      901 gcgcagggca aggtgcgcca cgtcggcgac caggtcgcgc tggtggtcgc cgaaacgctg
      961 ctgcaggcca aggacgcctg cgagctgatc gaggtcgact acgaggagct gcccgccgtc
     1021 atcgacatca cgcaggccga cggccatgcc ggccatgtgc acgacgacgt gccgaacaac
     1081 gtctgctacg actggggcca cggcaacaag gaggcggtcg acgccgcgtt cgccggcgct
     1141 gcgcatgtca gcacgctgga catcgtcaac aaccggctga tcccgaacgc gatggagccg
     1201 cgcgccgcga acgcgagcta cacgaagcac gacgagagct acacgctgta cgtctcgaac
     1261 cagaacccgc acgtcgagcg gctgctgatg tgcgcgttcg tgctcggcct gcccgaatcg
     1321 aaagtgcgcg tgatcgcgcc cgatgtcggc ggcggcttcg gctcgaagat cttcctgtat
     1381 cccgaagaga ccgcgctggt ctgggccagc aagcacgtcg gccgccccat caaatggacg
     1441 gccgatcgca gcgaggcctt cctgaccgat gcgcacggcc gcgaccacgt caccaaggtc
     1501 gagctggcgc tggacgcgaa gggcatgttc ctggcgctgc gcgtcaagac gatcgcgaac
     1561 ctcggcgcct acctgtcaac cttcgcgtcg agcgtgccga cgattcttta cgcgacgctg
     1621 ctggccggcc agtacaagac gccggcgatc tacgccgagg tcaaggcggt gttcaccaac
     1681 accgcgccgg tcgacgccta ccgcggcgcc ggccgccccg aggcgaccta cgtggtcgag
     1741 cgcatcgtcg aaacggcggc acgcgacctg aagctggacc cggtcgcgat ccggcgccag
     1801 aacttcatca ccgagttccc gtacgcgacg ccggtcggcc tgacctacga caccggcaac
     1861 tacgaagcgc atctggccaa ggcgatcgcg atggccgatg tggccgactt cgcggcgcgc
     1921 cgggccgcgt ccgaagccaa gggcaagaag cgaggcctcg gctacagctg ctacatcgag
     1981 gcctgcgggt tggcgccgtc gaacatcgcc ggtgcgctcg gcgcgcgcgc cggcctgttc
     2041 gaggccggcg aggtgcgcgt gcacccgacc ggcaccgtga cggtgttcac cggctcgcac
     2101 agccacggcc agggccacga gaccacgttc gcgcaggtcg tcgcggccaa gctcggcatc
     2161 ccggtcgaga gcatcgagat cgtgcacggc gacaccggcc gcgtgccgtt cggcatgggc
     2221 acctacggca gccgctcgtt gagcgtcggc ggcaccgcga tcgtcaaggc ggtcgacaag
     2281 at
//
LOCUS       DASRDG010000216         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_487442, whole genome shotgun sequence.
ACCESSION   DASRDG010000216 DASRDG010000000
VERSION     DASRDG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487442"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1224
                     /locus_tag="VFA35_03560"
     CDS             352..1224
                     /locus_tag="VFA35_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55278.1"
                     /translation="MASLPALERVAAGVWRVPAARGEPDAANGGATIQLVLVRDGARL
                     WLIGSGPTPAFGAALACAIERGTGRAVTDVVNTRAAPELALANAAFPAARLWALPDVA
                     ATMRARCGQCLARLQARIGPAGASLRPTLIRVPTRRVGTAGQRAGRLGPFDWLALARA
                     PGERVLVLRLRRPHTMRVVIAQGLLWAGDLPDLRDTRSDTFAASLRRLRTFGAGALLL
                     GEQGGPSGVATLTNHLAYLAVLHAAIVPQLAHGDAAAAQAPLPAFMHWPGYAATHPLN
                     VQRVWRELEPTLFR"
     gene            complement(1235..3325)
                     /locus_tag="VFA35_03565"
     CDS             complement(1235..3325)
                     /locus_tag="VFA35_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HZT55279.1"
                     /translation="MPAARAGAAAPSVAPRVEFIERSHQRSAALGLTRTENRGFDPMV
                     RSDLNLARERNQRLYLHAAPVMHMLFEQIVNTESMILLTDAQGTILHSVGDDDFMARA
                     DKVALAPGENWAEHAKGTNAIGTALFEEAPTLVHANEHFMLANSFLTCSAAPIFDPRG
                     NMLGVLDVTGDHRGYHQHTMGLVRMSARMIENHWLSDDYNNRLRLHFHSRAEFIGTLL
                     EGIIVVSPEGRILGANRGALDQLGIGGAELRAHSLTSLFGTTSAAVYDHFRMPMSAPM
                     QLYLPNGAQFFVSARFDWPSRPVVAGLGLADAGSATAPAPAAPALGDGAASATATALA
                     GHPGGPRFSGLQYLRTGDPQLDLLVQKIRRVLDRDIPLLILGETGTGKELLARAVHHD
                     SNRARQPFVAVNCASIPESLIEAELFGYEDGAFTGARRKGAPGRLVQANGGTLFLDEI
                     GDMPQPLQARLLRVLQERSVTPLGSTKSIAVDISVIGATHRNLREMIDRQEFREDLYY
                     RLNGLALRLPALRERSDLEAVAKRILQAERPHDTPELSAPVRELFRRYQWPGNIRQLA
                     NVLRTAAVMSVGERQITPLHLSDDFLEDARRGDPAPRTPPPLPVAPSADRIAAAGAQS
                     FLPPQATAPDARAAPEPAEARAPHTLEQAEIEMIRAALDAAGGNISAASKQLGISRNT
                     IYRKLRWNTPDRAG"
BASE COUNT          420 a         1211 c         1306 g          518 t
ORIGIN      
        1 aggccgcgct ggcgtggccg catgttcgtg tctccctttc ctgtcgccgc ttcttgtcgt
       61 cgtgccacag gcggcagggc gcttgcttgg caaggaggcc ttcgaccccg acccgagcgc
      121 cgagccgccg tgactgttgc ccttgttctg caatccctat gccggcaggc gatcgccggg
      181 aatcgacgcc tgcgcgcggc ggattgctcc aatccggagc agcgcgatgg aacgctggca
      241 cagggaaagc cctctgccgc cggctgttgc cgtgacgctg cacgttggta catctgcgca
      301 agcctcgcgt ggctccccgc gctcgccggc gccgcgtgtg ccgacgcgcc ggtggcgtcg
      361 ctgcccgcgc tggagcgcgt ggcggccggc gtctggcgcg tgcccgcggc gcgcggcgag
      421 cccgacgccg cgaacggcgg cgcgacgatc cagctcgtgc tggtgcgcga cggcgcgcgc
      481 ctgtggctga tcggcagcgg cccgacgccg gccttcggcg cggcgctcgc ctgcgcgatc
      541 gagcgcggca ccggccgcgc ggtgaccgac gtggtcaaca cccgcgccgc acccgagctg
      601 gcactcgcga acgccgcgtt cccggcggcg cggctgtggg cgctgcccga cgtggccgcg
      661 acgatgcgcg cgcgttgcgg ccaatgcctg gcgcggctgc aggcgcgcat cggcccggcc
      721 ggcgcgagcc tgcggcccac cttgatccgc gtgccgaccc ggcgcgtcgg cacggccggc
      781 cagcgcgccg gccgcctcgg cccgttcgac tggctggcgc tggcacgcgc acccggcgag
      841 cgcgtgctgg tgctgcgcct gcggcgaccg cacacgatgc gcgtggtgat cgcgcagggg
      901 ctgctgtggg cgggcgacct gcccgatctg cgcgacacgc gcagcgacac gtttgccgcc
      961 agcctgcgcc gcctgcgcac cttcggcgcc ggcgcgctgc tgctcggcga gcaaggcggc
     1021 ccgagcggcg tggccacgct gaccaaccac ctcgcctatc tggcggtgct gcacgcggcg
     1081 atcgtgccgc agctcgcgca cggcgacgcg gcagcggcgc aggcgccgct gcctgcgttc
     1141 atgcactggc cgggctatgc ggcgacgcac ccgctcaacg tgcagcgcgt ctggcgcgaa
     1201 ctcgagccga cgctgttccg ctgaaggtgc aaggtcagcc ggcgcggtcg ggcgtgttcc
     1261 agcgcagctt gcggtagatc gtgttgcggc tgatgccgag ttgcttcgac gcggccgaga
     1321 tgttgccgcc ggcggcgtcg agcgcggccc ggatcatctc gatctcggcc tgctcgagcg
     1381 tgtgcggggc gcgcgcctcg gccggctcgg gcgcggcacg cgcgtcgggc gccgtggcct
     1441 gcgggggcag gaagctctgc gcgccggcgg ccgcgatcct gtcggccgac ggcgccaccg
     1501 gcagcggcgg cggcgtgcgc ggcgcggggt cgccgcggcg agcgtcctcg agaaaatcgt
     1561 ccgacaggtg cagcggcgtg atctggcgct cgccgaccga catcacggcc gcggtgcgca
     1621 gcacgttggc gagctggcgg atgttgcccg gccactggta acgccggaac agctcgcgca
     1681 ccggcgcgct cagctcgggc gtgtcgtgcg gccgctcggc ctgcaggatg cgcttggcga
     1741 cggcctcgag gtcgctgcgt tcgcgcagcg ccggcagccg cagcgcgaga ccgttcaggc
     1801 ggtagtagag gtcttcgcga aactcctgcc ggtcgatcat ctcgcgcagg ttgcgatgcg
     1861 tcgcgccgat cactgagatg tcgaccgcga tcgacttggt gctgcccagc ggcgtcacgc
     1921 tgcgctcttg cagcacgcgc agcaggcgcg cctgcagcgg ctgcggcatg tcgccgatct
     1981 cgtcgaggaa cagcgtgccg ccgttggcct gcacgagccg ccccggcgcg cccttgcggc
     2041 gtgcgccggt gaacgcgccg tcctcgtagc cgaacagttc ggcctcgatc agcgactcgg
     2101 ggatcgaggc gcagttcacc gcgacgaagg gctggcgcgc gcggttcgag tcgtggtgca
     2161 cggcgcgcgc gagaagctcc ttgccggtgc cggtttcgcc gaggatcagc agcgggatgt
     2221 cgcggtcgag cacgcgccgg atcttctgca ccagcaggtc aagctggggg tcgccggtgc
     2281 gcaggtattg caagcccgag aaacgcggcc cgcctgggtg gcccgcgagg gccgtggccg
     2341 tggccgaggc ggcgccatcg ccgagcgccg gggcggcggg cgccggcgca gtcgccgaac
     2401 ccgcgtcggc caagccgagg ccggcgacga ccggccgcga aggccagtcg aaacgcgcgc
     2461 tgacgaagaa ctgcgcgccg ttcggcagat acagctgcat cggcgccgac atcggcatgc
     2521 ggaagtggtc gtagaccgcc gccgacgtgg tgccgaacag gctcgtcaag ctgtgcgcgc
     2581 gcagctcggc gccgccgatg ccgagctggt cgagcgcgcc acggttcgcg ccgaggatgc
     2641 ggccctcggg gctgacgacg atgatgcctt cgagcagcgt gccgatgaac tcggcgcggc
     2701 tgtggaagtg caggcgcagc cggttgttgt agtcgtcgga gagccagtgg ttctcgatca
     2761 tccgcgccga catccgcacc agccccatcg tgtgctggtg gtagccgcgg tggtcgccgg
     2821 tcacgtcgag cacgccgagc atgttgccgc gcgggtcgaa gatcggcgcg gccgaacagg
     2881 tcaggaagct gttcgcgagc atgaagtgct cgttcgcgtg caccagcgtc ggcgcctcct
     2941 cgaacagcgc ggtgccgatc gcgttcgtgc ccttggcgtg ctcggcccag ttctcgcctg
     3001 gcgcgagcgc gaccttgtcg gcgcgcgcca tgaagtcgtc gtcacccacc gagtgcagga
     3061 tcgtgccctg cgcgtcggtc agcaggatca tgctttcggt gttgacgatc tgctcgaaca
     3121 gcatgtgcat gaccggcgcg gcgtgcaggt acaggcgctg attgcgttcg cgcgcgaggt
     3181 tcaggtcgct gcgcaccatc gggtcgaagc cgcgattctc ggttcgcgtc aggccgagcg
     3241 cggcgctgcg ctggtgcgaa cgctcgatga actcgacgcg cggcgcaacg ctcggcgccg
     3301 cggcgcccgc gcgcgccgcg ggcacggggc gggtggggtc gcgtcgctct gagctcatag
     3361 gctacctctg caatggcagg ttgtctcgat cgtcggtcgt gtatcagcac cgttgcgggc
     3421 ggtgccgccg acctcttgtg cgcgcatggc ttcga
//
LOCUS       DASRDG010000217         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1638006, whole genome shotgun sequence.
ACCESSION   DASRDG010000217 DASRDG010000000
VERSION     DASRDG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638006"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2487)
                     /locus_tag="VFA35_03570"
     CDS             complement(<1..2487)
                     /locus_tag="VFA35_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZT55280.1"
                     /translation="MEALHLMLAGATAVRRGDGAVKPLAPIDAALLAFVALEGATSRE
                     RLLGLLWPEQPPESARNVLRQRLFRLRRGLGAGVLAQGAELLALAPGVTHDLDDGPGE
                     LLAGLHFPECDELDAWLVAQRAALKQRQRQQQEARIDALERDGRLAEGSEIAERLVSA
                     DPLDEAAARRLMHLRYLQGERAAALAVYDRFAAALAEALEAKPARSTCELRETIRAAP
                     APQPIARRDIPTSLLRPPRLVGRSRERAALATAWAARRAFWLLGEAGLGKTRLISEFA
                     AEATAGQAAAQVLLVSARPGDAGVPYASLGRALRAVIGRAPSVLEPAQRDELARVLPE
                     IGPVVVAAAGATRRPTLFAALEALLLDARADGLAALVIDDLHFADSASLEMLQGLVLA
                     ESLADLRWGFAQRPAEGEAAVEALATALEEAHRVEFVALGALDEPAMRELVESLGLDG
                     HDPARLAPLLVRHTGGNPLYALETLKHLLATGATSLDAGLPRPPSIRHLIERRLRQLS
                     PAAIALARIAALAGVDFSIELAEQVLATRALALADPWRELEEAQVLRDHAFAHDLVLE
                     ATLAGVPAPIAAHAHQAIAEFLEAHGHAPARVASHWIASGQAQRARAALHAAADAARQ
                     AMRRKEEAGFLSRVAQIEAESGDAAQFESLRAMIEALWIADRGGLDAATYERFAAAAT
                     TAQQRGVVLGLRAIWVHEQGDVEGAKQLCRQSIELAEATGDEATAAQAREQLALFLHF
                     SGECDAAVALLQQLQPWISEHASDARKVDFYGDLGIVLDNADRGREARTYHKLSIDLA
                     RRIGAWSEVVTVLANLAISWATAGYMARSIE"
BASE COUNT          344 a          952 c          981 g          349 t
ORIGIN      
        1 ctcgatcgag cgcgccatgt aacccgccgt cgcccagctg atggcgaggt tggccaggac
       61 ggtcaccacc tcgctccagg cgccgatgcg ccgcgccagg tcgatcgaga gcttgtgata
      121 cgtgcgcgcc tcgcggccgc ggtcggcgtt gtcgagaacg atgccgagat cgccgtagaa
      181 atcgaccttg cgtgcgtcgg acgcgtgctc cgagatccac ggctgcagtt gctgcagcag
      241 ggcgacggcg gcgtcgcact cgccgctgaa atgcaggaac agcgcgagct gctcgcgcgc
      301 ctgcgccgcg gtggcttcgt cgcccgtggc ttcggcgagc tcgatcgact gccggcagag
      361 ctgctttgcg ccctcgacgt cgccttgctc gtgaacccag atggcacgca gtccgaggac
      421 cacgccgcgc tgctgcgccg tcgtcgccgc ggcggcgaag cgctcgtagg tcgccgcgtc
      481 gaggccgccg cggtcggcga tccagagcgc ctcgatcatg gcgcgcaggc tctcgaactg
      541 cgcggcgtcg ccgctttcgg cctcgatctg tgcgacgcgc gaaagaaatc ccgcctcttc
      601 cttgcgccgc atcgcctgcc gcgccgcgtc ggccgcggcg tgcagcgcgg cgcgagcgcg
      661 ttgcgcctgg ccggaggcga tccagtgcga agcgacgcgc gccggcgcat ggccgtgagc
      721 ctcgaggaat tcggcgatcg cctggtgcgc atgcgccgcg atcggcgccg gcacgccggc
      781 caaggtcgcc tccagcacca ggtcgtgggc gaacgcgtgg tcgcgcagga cctgcgcctc
      841 ctcgagctcg cgccacgggt cggcgagcgc cagggcgcgg gtcgcgagca cctgctcggc
      901 aagctcgatg gagaagtcga cgccggcgag cgcggcgatg cgcgccagcg cgatcgccgc
      961 cggcgagagt tgcctgaggc ggcgctcgat caggtggcga atgctgggcg gtcgcggcag
     1021 gcccgcgtcg agcgaggtcg cgccggtggc gagcaggtgc ttgagggtct ccagcgcata
     1081 gagcgggttg ccgccggtgt ggcggacgag cagcggcgct agccgcgccg ggtcgtggcc
     1141 gtcgaggccg agcgattcga ccagctcgcg catcgccggt tcgtcgaggg cgccgagggc
     1201 gacgaactcg acccggtggg cttcctcgag cgccgtcgcc agggcttcga ccgcggcttc
     1261 gccctcggcc ggacgctgcg cgaagcccca gcgcagatcg gcgagactct cggcgagcac
     1321 cagcccctgc agcatctcga ggctggcgct gtcggcgaag tgcaggtcgt cgatgaccag
     1381 cgccgccaag ccatcggcac gggcatcgag cagcagggcc tcgagcgcgg cgaacagcgt
     1441 cgggcgccgg gtggcgcccg ctgcagcgac gacgacagga ccgatttccg gcagcacccg
     1501 cgccagctcg tcccgctgcg ccggttcgag cacggacggg gcgcgtccga tcacggcccg
     1561 cagcgcccgg ccgaggctgg cgtagggcac gccggcatcg cccggccgcg ccgagacgag
     1621 gagaacctgc gccgcggcct gcccggccgt ggcctcggcc gcgaattcgc tgatcaaccg
     1681 cgtcttgccc aggccggctt cgccgagcag ccagaaggcg cgccgtgcgg cccaggcggt
     1741 cgcgagcgcg gcacgttcgc gactccggcc gacgaggcgc ggcgggcgca gcaggctggt
     1801 cggaatgtcg cgccgcgcga tcggctgcgg cgccggcgcg gcgcggatcg tctccctcag
     1861 ctcgcaggtg ctccgcgccg gctttgcctc cagcgcttcc gcgagcgcgg cggcgaaccg
     1921 gtcgtacacg gccagcgccg ccgcccgttc gccctgcagg tagcgcaggt gcatcaggcg
     1981 gcgcgccgcg gcctcgtcga gcgggtcggc cgagaccagg cgctccgcga tctcgctgcc
     2041 ttcggcgagc cggccatcgc gttcgagcgc gtcgatgcgg gcctcctgct gctggcgctg
     2101 gcgctgcttc aacgcggcgc gctgtgcgac cagccatgcg tcgagctcgt cgcattcggg
     2161 aaagtgcagg ccggcgagga gctcgccggg gccgtcgtcg agatcgtggg tgacgccggg
     2221 cgccagcgcc agcagctcgg caccttgcgc caggacgccg gcgccgagcc ctcggcgcag
     2281 gcggaacagt cgctggcgca ggacgttgcg cgcgctttcg ggcggctgct ccggccagag
     2341 cagaccgagc agccgttcgc gcgacgtcgc cccttcgagg gcgacgaagg cgagcagagc
     2401 cgcgtcgatc ggcgccagcg gcttgaccgc gccgtcgccg cggcgcaccg ccgtcgcgcc
     2461 ggcgagcatc agatgcaggg cttccacccc tgtcatctga tgcgatcgcg gtcttcgcgt
     2521 caatggcgat ccgcctcagg gaacgaggca gcccgactgg tcgacgccgg cgacgacaca
     2581 accgccgacg atcaggttct tgctctgcgt gcagaacttc gccccg
//
LOCUS       DASRDG010000218         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_264167, whole genome shotgun sequence.
ACCESSION   DASRDG010000218 DASRDG010000000
VERSION     DASRDG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264167"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1807)
                     /locus_tag="VFA35_03575"
     CDS             complement(248..1807)
                     /locus_tag="VFA35_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZT55281.1"
                     /translation="MPIPSHALPADADGLLELAARSMFDTLANAAMGMFVIDRAHRIV
                     WISEGYKQFLPALEREEADFVGRRVEEVVPNTLMAQVVDTGQAMLVDLLTNKAGTFLV
                     SRLPLRNADGTVIGAIGMVLLDHPETTMRPLTAKFAQLERELASAREELAAHLTQQRR
                     PKHTIASFVGASPAAVEVKRQARRVAQTDSTVLLLGETGTGKELLAHGIHAASRRAAK
                     PFIAVNIAAVPETLLEAEFFGVAPGAYTGAERKGRDGKFKLADGGTLLLDEIGDMPLA
                     LQSKLLRVLQEQEVEPLGSNAVQRIDVRIIAATSRDLPAMVAAGSFRADLFYRLNVLP
                     IRLPALRERPTDLEALVDSLSDDIARRSGLAHKTLSADALDLLGRQRWPGNIRELRNA
                     LEQASLMTDDLLLTAAHFASLAIEAHEVVSAPAPRAPAPAPAPEPALGGVAVKPLSEA
                     VAELEGRAIRAAMAATGGNKLAASRLLGISRATLYEKLALAQREPARRLRIATDVDVD
                     VQAPPRLDSSG"
     gene            1866..2654
                     /locus_tag="VFA35_03580"
     CDS             1866..2654
                     /locus_tag="VFA35_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZT55282.1"
                     /translation="MSGIVHFQPAGSDGIAHVVLDNPGKLNAIDIAMWRALRRGFDAL
                     QALPAHTAPRAVIVAGAGGNFASGGDIVEFASFRFDAEPLRDFHEHTVAPALHAMLAC
                     DIPLFAQIDGACIGGGFEIAACCDIRVAGASSRFGAPIAKLGFPMAPGELELLARIAA
                     PAVLREMLLEARLFDAARALQLGIVHAVVADAELAAGTLQRARAAAALAPQAARINKR
                     TLRQLAAGGPDPAERLAHFDYAAGAEHREGVTAFIEKRAARFRP"
     gene            2651..>3131
                     /locus_tag="VFA35_03585"
     CDS             2651..>3131
                     /locus_tag="VFA35_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZT55283.1"
                     /translation="MSTHASTNANLFSALRAGFPADLDAIAIETADASADTAPLCYSW
                     RDLDRATAMLANLLGSLELPAGSRVAVQTEKSVEALMLYLAVLRAGLVYLPLNTAYQR
                     DEIAYFIGNAEPAVVVCSPKNFGWISRIAFAAGTAHVFTLGDDRSGRLLQRAAVHSDV
                     "
BASE COUNT          417 a         1128 c         1136 g          450 t
ORIGIN      
        1 cctttgtggt tgcaagcagg gcgcgagcgc gcgcatgcgc cgcggtcacg cgaccgcggt
       61 ggcagagcat tcgaagagga tgagcggcag gtcggggcac gctggctgcc gatggcggcg
      121 cctgactgcc cacacggggc aagaacgtcc cgatgacgcg ctccgatcct ggagaaccat
      181 cggctgtgcc cgtgcgcggc cagggtctcg ggtcgagacc gatccacacg ggatacggca
      241 cgcagggtca gcccgatgag tctaggcgcg gtggggcctg cacgtcaacg tcgacatcgg
      301 tcgcgatgcg cagccgacgc gcgggttcgc gctgtgcgag cgcgagcttc tcgtacagcg
      361 tcgcgcgcga aatgccgagc aggcgcgagg ccgcgagctt gttgccgccg gtcgcggcca
      421 tcgccgcgcg gatcgcgcgg ccttcgagtt cggcgaccgc ctccgacagc ggcttgaccg
      481 cgacgccgcc cagcgccggc tcgggtgccg gcgccggcgc cggcgcccgc ggtgccggcg
      541 cggacaccac ttcgtgcgcc tcgatcgcga gcgacgcgaa gtgcgcggcg gtcagcagca
      601 ggtcgtcggt catcaggctc gcctgttcga gcgcattgcg cagctcgcgg atgttgccgg
      661 gccagcgctg ccggccgagc aggtcgagcg cgtcggccga caaggtcttg tgcgccaggc
      721 cgctgcggcg cgcgatgtcg tccgagagcg agtcgaccag cgcttccagg tcggtgggcc
      781 gctcgcgcag cgccggcagg cggatcggca gcacgttcag gcggtagaac aggtcggcgc
      841 ggaagctgcc ggccgcgacc atcgccggca ggtcgcggct cgtcgcggcg atgatgcgca
      901 cgtcgatgcg ctgcaccgcg ttgctgccga gcggctcgac ctcctgctcc tgcagcacgc
      961 gcagcagctt gctctgcagc gcgagcggca tgtcgccgat ctcgtcgagc agcagcgtgc
     1021 cgccgtcggc gagtttgaac ttgccgtcgc ggcccttgcg ctcggcgccg gtgtaggcgc
     1081 ccggcgcgac gccgaagaac tcggcttcga gcagcgtctc gggaacggcc gcgatgttga
     1141 ccgcgatgaa gggtttcgcg gcgcgccggc tcgccgcgtg gatgccgtgc gcgagcagct
     1201 ccttgccggt gccggtctcg cccagcagca gcaccgtgct gtcggtctgc gcgacgcgcc
     1261 gcgcctggcg cttgacctcg accgcggccg ggctcgcgcc gacgaagctc gcgatcgtgt
     1321 gcttgggccg gcgctgctgc gtgaggtgcg cggccagctc ttcgcgcgcc gacgcgagct
     1381 cgcgctcgag ctgcgcgaac ttggcggtca gcgggcgcat cgtcgtctcg gggtggtcga
     1441 gcagcaccat gccgatcgcg ccgatcacgg tgccgtcggc attgcgcagc ggcagccggc
     1501 tgacgagaaa ggtgccggcc ttgttggtca gcaggtcgac gagcatcgcc tggccggtgt
     1561 cgaccacctg cgccatcagc gtgttcggca cgacctcctc gacgcggcgg ccgacgaaat
     1621 cggcctcctc gcgttcgagc gccggcagga attgcttgta gccctcgctg atccagacga
     1681 tgcggtgcgc gcggtcgatc acgaacatgc ccatcgccgc gttcgccagc gtgtcgaaca
     1741 tgctgcgcgc ggcgagttcg agcaggccgt cggcgtcggc cggcagggcg tgggagggga
     1801 tgggcatgcg ggcggtgcgg atggtccggc gaactgtagc gcggccgggg cgcgacaatc
     1861 ggcgcatgag cggcatcgtc catttccaac ccgcgggcag cgacggcatc gcgcacgtcg
     1921 tgctcgacaa ccccggcaaa ctcaatgcga tcgacatcgc gatgtggcgc gcgctgcgcc
     1981 gcggcttcga cgcgctgcag gcgctgccgg cccacacagc accgcgcgcg gtgatcgtcg
     2041 ccggcgccgg cggcaacttc gcgtcgggcg gcgacatcgt cgagttcgcg agcttccggt
     2101 tcgacgcgga gccgctgcgc gacttccacg aacacacggt cgcgccggcc ttgcacgcga
     2161 tgctggcctg cgacatcccg ctgttcgcgc agatcgacgg cgcctgcatc ggcggcggct
     2221 tcgagatcgc ggcctgctgc gacatccgcg tggccggcgc gtcgagccgc ttcggcgcgc
     2281 cgatcgcgaa gctgggtttt ccgatggcgc cgggcgagct cgagctgctc gcgcgcatcg
     2341 cggcgccggc ggtgttgcgc gagatgctgc tcgaggcgcg gctgttcgac gcggcgcgcg
     2401 cgttgcagct cgggatcgtg catgcggtcg tcgccgatgc cgagctcgcc gcgggcacgc
     2461 tgcagcgcgc gcgcgccgcg gccgcgctcg cgccgcaggc cgcgcgcatc aacaagcgca
     2521 cgctgcgcca gctcgccgcg ggcggccccg accccgccga acgccttgcc cacttcgact
     2581 acgccgccgg cgccgaacac cgcgaaggcg tgacggcctt catcgagaaa cgcgccgccc
     2641 gcttccggcc atgagcaccc acgccagcac caacgccaac ctcttcagcg cgctgcgcgc
     2701 cggctttccc gccgacctcg acgcgatcgc gatcgagacc gccgacgcca gcgccgacac
     2761 cgcgccgctg tgctactcgt ggcgcgacct cgaccgcgcc accgcgatgc ttgcgaacct
     2821 gctcggctcg ctcgagctgc ccgcgggcag ccgggtcgcg gtgcagaccg agaaatcggt
     2881 cgaagcgctg atgctgtacc tcgcggtgct gcgcgccggc ctggtctacc tgccgctgaa
     2941 caccgcctac cagcgcgacg agatcgccta cttcatcggc aacgccgagc cggcggtcgt
     3001 cgtctgttcg ccgaagaact tcggctggat cagccggatc gcgttcgccg ccggcaccgc
     3061 ccacgtcttc acgctcggcg acgaccgcag cggcagactg ctgcaacgcg ccgcggtgca
     3121 cagcgatgtg c
//
LOCUS       DASRDG010000219         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_837473, whole genome shotgun sequence.
ACCESSION   DASRDG010000219 DASRDG010000000
VERSION     DASRDG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837473"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFA35_03590"
     CDS             <1..775
                     /locus_tag="VFA35_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZT55284.1"
                     /translation="TERHVIIAGYGRSGQNLARILEGEHIPYMALDLDPDRVRQAAAA
                     GQNVVFGDAARLQSLMAAGLARASAVVVSYHDVASALKILHLVQEHAPRVPVIVRTID
                     DTDIERLQAAGATEVVPEEIEGSLMLASHALALVGVPMRRVIRVVQEQRDARYGLLRG
                     YFHGADDDTVDELQTARLLSVTLPAAGNHLGRDLAGLALDALGVAVVSVRRANGAVVK
                     PDDALVLAGGDTLVLAGRPEALALAEGRLLGKSKKQKQE"
     gene            complement(809..1276)
                     /locus_tag="VFA35_03595"
     CDS             complement(809..1276)
                     /locus_tag="VFA35_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZT55285.1"
                     /translation="MEIDQLVQAIQTLNAEDAFRARLSLEQWRSIVPYLTRHEIRAGD
                     LLIKQGDTDRTMYFLAQGSLQVFVTGGPPGSNRIAILRTGSAVGEPGLFGDNPRMANV
                     EAMTPCVVYALRGPRLEELAQRLPALALDLLRAAGGVMATRMRANLAKQTPFT"
     gene            complement(1434..2825)
                     /gene="mnmE"
                     /locus_tag="VFA35_03600"
     CDS             complement(1434..2825)
                     /gene="mnmE"
                     /locus_tag="VFA35_03600"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZT55286.1"
                     /translation="MLARHHDPIVAVATASGRGAVGIVRVSGADLSAFVVALLGRALQ
                     PRHASYLPFNDAAGAPIDHGLAIHFPAPHSYTGEDVLELQAHGGPVVLQLLLARCLEL
                     ANAQLPGLRLARPGEFTERAFLNDKLDLAQAEAVGDLIEASTEAAARSAGRSLTGAFS
                     DEVNALRDQLIALRMLVEATLDFPEEEIDFLEKANARGRLQAIAAQVDTVLDRARQGA
                     LLREGITVVLAGQPNVGKSSLLNALAGAELAIVTPIAGTTRDKVSSTIQIEGVPLHVI
                     DTAGLRDSTDAVEKIGVARSWSEIERADAVLLLHDLTRLGEPAYDAGEAQIEARLPAA
                     LRHSERLLHVFNKSDAVPAASAAVPAAGLALSARTGAGIDALRAKLLALAGWHAPPEG
                     LFIARTRHVQALRETRAHLAAALAQAARADAALDLLAEELRLAHERLGEITGAFTSDE
                     LLGEIFSRFCIGK"
     gene            complement(2830..>3206)
                     /gene="yidC"
                     /locus_tag="VFA35_03605"
     CDS             complement(2830..>3206)
                     /gene="yidC"
                     /locus_tag="VFA35_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZT55287.1"
                     /translation="KVNPIGGCLPIFLQMPFFIALYWVLLSSVEMRNAPWIGWITDLS
                     AKDPYFILPILMTLTSLLQTWLNPTPPDPMQAKMMWIMPLMFSVMFFVFPSGLVLYWL
                     TNNILSIAQQYVINKRLGVLNQ"
BASE COUNT          464 a         1106 c         1177 g          459 t
ORIGIN      
        1 taccgagcgc cacgtgatca tcgcgggcta cggccgcagc gggcagaacc tcgcgcgcat
       61 cctcgagggc gagcacatcc cgtacatggc gctcgacctc gaccccgacc gggtgcgcca
      121 ggccgccgcg gccggccaaa acgtggtgtt cggcgacgcc gcgcgcctgc aaagcctgat
      181 ggccgcaggc ctggcgcgcg ccagcgcggt cgtcgtcagc taccacgacg tggcctcggc
      241 gctgaagatc ctgcacctcg tgcaggagca cgcgccgcgc gtgccggtga tcgtgcgcac
      301 gatcgacgac accgacatcg agcggctcca ggccgccggc gcgaccgagg tcgttcccga
      361 ggagatcgag ggctcgctga tgctcgcgag ccacgcgctc gcgctcgtcg gcgtgccgat
      421 gcggcgcgtg atccgcgtcg ttcaggagca gcgcgacgcg cgctacggcc tgctgcgcgg
      481 ctacttccac ggcgccgacg acgacacggt cgacgagctc cagacggcgc ggctgctgag
      541 cgtgacgctg ccggccgcgg gcaaccacct cgggcgagac ctggcggggc tggcgctgga
      601 tgcgctcggc gtcgcagtgg tgtcggttcg gcgcgccaat ggcgcggtcg tgaagcccga
      661 cgacgcgctg gtgctcgccg gcggcgacac gctggtgctc gccggcaggc ccgaggcgct
      721 ggcgctggcc gaggggcggc tgctcggcaa gagcaagaag cagaagcagg agtagtcgag
      781 gccgggatcg aggccaagac cgtcgctgct acgtgaacgg cgtctgcttg gcgaggttcg
      841 cgcgcatgcg cgtggccatc acgcccccgg cggcgcgcag cagatcgagc gccagcgccg
      901 gcaggcgctg cgccaactct tcgaggcgcg ggccgcgcag cgcgtagacc acgcacggcg
      961 tcatcgcctc gacattggcc atgcgcgggt tgtcgccgaa caggcccggc tcgccgacgg
     1021 ccgagccggt gcgcaggatc gcgatgcggt tgctgccggg cgggccgccg gtgacgaaga
     1081 cctgcaggct gccctgcgcg aggaagtaca tcgtgcggtc ggtgtcgccc tgcttgatca
     1141 gcaggtcgcc cgcgcgaatc tcgtggcgcg tgaggtaggg cacgatgctg cgccactgct
     1201 cgaggctcag gcgcgcgcgg aaggcgtcct cggcgttgag cgtctgaatg gcttgcacga
     1261 gctggtcgat ttccatctgc ctcctccgcg cagcgccccg gccgcggggc gcttgtcggc
     1321 tcgattatcg cggcctgccg cagcgcctca caaccgccgc agcatgcggc cagcgccctt
     1381 catcgccgtc ggccgcccgt tcgtcggctt gcgtgccacg cgccgacgcg ggcttacttg
     1441 ccgatacaga agcggctgaa gatctcgccg agcagctcgt ccgacgtgaa cgcgccggtg
     1501 atctcgccga ggcgctcgtg ggcgaggcgg agttcttcgg cgagcaggtc gagcgcggcg
     1561 tcggcgcgcg cggcctgcgc aagcgcggcg gccaggtgcg cgcgcgtctc gcgcagggcc
     1621 tgcacgtggc gcgtgcgcgc gatgaacagg ccctcgggcg gcgcgtgcca gccggcgagc
     1681 gcgagcagct tcgcgcgcag cgcatcgatg ccggcgccag tgcgggccga aagcgcgagg
     1741 ccggcggcgg gcaccgcggc cgatgccgcc ggtacagcgt ccgacttgtt gaacacgtgc
     1801 agcaggcgct cgctgtgccg cagcgcggcg ggcaggcgtg cctcgatctg cgcctcgccg
     1861 gcgtcgtagg ccggctcgcc gaggcgcgtc aggtcgtgca gcagcagcac cgcgtcggcg
     1921 cgctcgatct cgctccagct gcgcgcgacg ccgatcttct cgaccgcgtc ggtcgagtcg
     1981 cgcaggccag cggtgtcgat cacgtgcagc ggcacgccct cgatctggat cgtgctgctg
     2041 accttgtcgc gcgtggtgcc ggcgatcggc gtgacgatcg cgagctcggc cccggccaac
     2101 gcgttcagca acgagctctt gcccacgttc ggctggccgg cgagcacgac cgtgatgccc
     2161 tcgcgcagca gcgcgccctg gcgcgcgcgg tcgagcaccg tgtcgacctg cgcggcgatc
     2221 gcctgcagcc ggccgcgcgc gttggccttc tcgaggaagt cgatctcttc ttcggggaag
     2281 tcgagcgtcg cctcgaccag catgcgcagc gcgatgagct ggtcgcgcag cgcgttgacc
     2341 tcgtccgaga acgcgccggt gagcgaacgc ccggccgagc gcgcggccgc ctcggtgctc
     2401 gcttcgatca ggtcgccgac ggcctcggcc tgcgcgaggt cgagcttgtc gttgaggaac
     2461 gcgcgttcgg tgaactcgcc gggccgggcc aggcgcagac ccggcagctg cgcgttggcg
     2521 agttcgaggc agcgcgcgag cagcagctgc agcacgaccg ggccaccgtg ggcctgcagt
     2581 tcgagcacgt cttcgccggt gtatgagtgc ggcgccggga agtggatcgc caggccatga
     2641 tcgatcggcg cgccggccgc gtcattgaac ggcaggtagc tcgcgtggcg cggctgcagc
     2701 gctctgccga gcagtgcgac gacgaaggcc gagagatcgg cccccgaaac ccgcacgatc
     2761 ccgaccgcgc cgcgcccgct cgccgtcgcg acggcgacga tcgggtcgtg atggcgcgcc
     2821 agcatcgcgt tattggttga gcacgcccag ccgcttgtta atcacgtact gctgcgcgat
     2881 cgacaggatg ttgttggtga gccagtacag caccaggccg gacgggaaca cgaagaacat
     2941 caccgagaac atcagcggca tgatccacat catcttggcc tgcatcggat cgggcggcgt
     3001 cgggttcagc caggtctgca gcaggctggt cagcgtcatc aggatcggca ggatgaagta
     3061 cgggtctttc gccgacaggt cggtgatcca gccgatccac ggcgcgttgc gcatctccac
     3121 gctcgacagc agcacccagt agagggcgat gaaaaaaggc atctgcagga agatcggcag
     3181 gcagccgccg atcggattga ccttct
//
LOCUS       DASRDG010000220        10539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_837578, whole genome shotgun sequence.
ACCESSION   DASRDG010000220 DASRDG010000000
VERSION     DASRDG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10539
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837578"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="VFA35_03610"
     CDS             complement(<1..1718)
                     /locus_tag="VFA35_03610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HZT55288.1"
                     /translation="MNLLLRSTLALAATLLVAAGASAQNAPPPTLGSGIDRSLIDPSV
                     RPQDDLYAHANGRWLKSAVFPPDKAYIGAAEQMHEATQAQLRELVEEAGRRRGDREAV
                     KIGDFYASFMDEAAIEKLGLAPLAAELALVDAVADTRQFAALMPRLARLGAGMPIGMS
                     VGQDDRDATRYVPSLVQSGLGLPNRDYYLKTDDAKFAGVRAKYVEYLAKLLTLAGDKV
                     GAGTAARAVLALETELARVQWSAVENRDPIKAYNRIELAALPALAPGLDWPAYLDSAR
                     LVGKTPDVLVRQPSYLTGMAALLDATPLATWKAYARVRLVGAYAPFLSKAFVDTRFAF
                     AGTVLSGTLEDRPRWKRGIAIVDQSIGEGLGKLYVAKHFPPESKRRMDELVANLLAAC
                     RDSIATLDWMTPATRREALAKLAKFNAKIGYPKRWIDYAALQVRRDDLVGNVMRARAF
                     ENARELAKLGKPIDRDEWGMTPQTINAYYDASLNEIVFPAAFLQAPNFDAAADDAANY
                     GAIGAVIGHEISHGFDDKGSQFDGAGLLRLWWTPEDRERFDAKTRILIDAYSVFSPVP
                     GYTINGA"
     gene            complement(1872..4094)
                     /locus_tag="VFA35_03615"
     CDS             complement(1872..4094)
                     /locus_tag="VFA35_03615"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HZT55289.1"
                     /translation="MNARTTDRTPVHGLLVATALHRFVEEQVLPGTGVDAAAFWKGFD
                     SIVADLAPKNAALLAERERLQAELDAWHKKHPGPIKNPAKYRAFLQKIGYLVPVPPVV
                     KATTKNVDAELALQAGPQLVVPITNARYALNAANARWGSLYDALYGTDAISEADGAEK
                     GAGYNPVRGAKVIDYARHVLDRTAPLEKGSHVDSTGYRVEGGALVVTLADGTTTGLAK
                     PAQFVGYQGAAATPSAVLLQHHGLHLDLRIDRSTAIGKNDPAGVSDLILESALSTILD
                     LEDSVAVVDADDKVQAYGNWLGILKGTLTEEVSKGGKTFTRGLNPDRVYIGPQGEDVR
                     LHGRSLLFVRNVGHLMTNPAILWGADAQEIPEGIMDAVITTAIAIHDLKRRGEGSAGA
                     AGIVNSRKGSVYIVKPKMHGPAEVAFAAELFGRVEQLLGLPKATVKLGIMDEERRTSV
                     NLKACIAAAADRVAFINTGFLDRTGDEMHTAMHAGPMIRKGDMKSSAWIAAYERSNVL
                     VGLSCGLRGKAQIGKGMWAMPDLMAAMLQQKIVQPRAGANTAWVPSPTAATLHALHYH
                     QVDVLAVQKALEKTDAAAAAPAILEGLLTVPVVKKAKWSETERQQELDNNAQGVLGYV
                     VRWVDQGVGCSKVPDIHNVGLMEDRATLRISSQHMANWMLHGVVSKKEVNATLKRMAK
                     VVDQQNAGDPLYQPMAGRFKESAAYRAAHDLVFKGLTQPSGYTEPLLHAWRLKVKAAA
                     "
     gene            4250..5161
                     /locus_tag="VFA35_03620"
     CDS             4250..5161
                     /locus_tag="VFA35_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZT55290.1"
                     /translation="MDKLKQLQAFASVATRGSLTAAAAAEGVAPAVIGRRIDALEARL
                     GVKLLVRTTRRISLTHEGSAFLEDCQRLLADWANAEASVSAGGVKASGHLRITSPAGF
                     GRRHVAPLVPRFLALHPEVSLSLNLSDRVVDIVNESFDCAVRVGELPDSSLVSVRLAD
                     NRRLCVATPGYLKRAGTPMHPAELARHECLALSSDASQTRGWAFSVDGGVIRWRPRGR
                     LDCTDGQVLHAWCSAGLGIAWRSAWEVEQEIAAGELVSVLDAFAAPPNGVYAVFPHAK
                     HLALRVRLWIDFLKHSYGDPAYWARRN"
     gene            complement(5203..5496)
                     /locus_tag="VFA35_03625"
     CDS             complement(5203..5496)
                     /locus_tag="VFA35_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HZT55291.1"
                     /translation="MKHLMTVLALSVACLSTSAFAADAASAPMGKQQTKMGECNKDAA
                     DKKGDERKAFMKTCLSAKKATQQEKMKACNADPKAKASKGDERKAFMKECLSK"
     gene            complement(5615..6964)
                     /locus_tag="VFA35_03630"
     CDS             complement(5615..6964)
                     /locus_tag="VFA35_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZT55292.1"
                     /translation="MRFDPGPPPDAPALPSDIGKYRVLERLGEGATAEVFLCHDDFKD
                     IDVAIKRVRPNFSGDAMDGRYGERFFAAEAALVGRLNHPNVVQIFDAVADPVAPYLVM
                     EYVAGSTLRPYCRADQLLGLELIVEIGFKCAMALGYVYRQGLIHRDVKPANLLAVLNN
                     GMVTDVKISDFGSVLNLSADTTQIYRVGSLAYMSPEQLDGGALDARADMYSLGAVLYH
                     LIAGRPPFDAQVQSAMMHQIYHLAPASLATLREGVSPGLNAMIQRALAKNPAERYANW
                     EEFAQALSGLITSRQVPRGQLQGVLDSERFNLLRSLDFFASFGDVELWEVVHRAKWQR
                     FRFGHALYRKGEEGNSFHILAQGEVEVYRDGKKVAQLGSGASIGEMVYLAPNPELKRH
                     STDVIVTEPATTISFTPETLMQVSPGCRHLFDEAFIRVLVRRLHAAHEALAHPRRIL"
     gene            7124..8836
                     /gene="argS"
                     /locus_tag="VFA35_03635"
     CDS             7124..8836
                     /gene="argS"
                     /locus_tag="VFA35_03635"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZT55293.1"
                     /translation="MIQAKQDLLAALGAAIKEVAPQASLAPAFESPKQAAHGDLACTA
                     AMPLAKALKKNPRELASALVDALQRQPAVQAWVDAVEIAGPGFINLRLKPAAKQAVVA
                     EINQGGDCFGRQPSKPDRMLVEFVSANPTGPLHLGHARQAALGDAICNLFETQGWQVS
                     REFYYNDAGVQIATLANSVQARLRGLGPGDAGWPEPAYHGEYIADIAADFLAKQGVKA
                     DDREFTASGDVDDLDAIRQFAVAYLRHEQDLDLRAFGVRFDHYFLESSLYASGQVDAT
                     VQRLIESGKTFEDGNALWLRTTDYGDDKDRVMRKQDASYTYFVPDVAYHLDKHARGFG
                     KAINIQGGDHHGTVARVRAGLQAANRGVPAGYPEYVLHKMVTVMKGGAEVKISKRAGS
                     YVTLRDLIDWTSRDAVRFFLLSRKADSEFTFDVDLALQRNDENPVFYVQYAHARICSV
                     IRHFAEQGGTLGSTPDLALLTAPTEAALMLELAEFPAMLANAAAGLAPHDLTFYLRDV
                     AGAFHSYYAAERVLVDDPALARARIALLVATRQVIRNALAVLGVSAPERMDRDTPESK
                     PEST"
     gene            8833..9525
                     /locus_tag="VFA35_03640"
     CDS             8833..9525
                     /locus_tag="VFA35_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZT55294.1"
                     /translation="MNDRKKSQRGGFAVGLVIGLLIGLALALGVALYVTKVPIPFVNK
                     VPQRSADQDAAETQKNKNWDPNSPLYGKNPAKPNGAASGTVAPLPPVATPQPGALPAP
                     AVTGSVTPAAPRASAPSSARDPAAILADKPAAPMPAPKITASASAAGGTDPFSYFVQA
                     GAYGRTEDAEAQRAKLAMLGMEGRLTEREQAGRTVYRVRLGPFEKKADAEAAKERLAG
                     AGVEAALVRVQR"
     gene            9612..10262
                     /locus_tag="VFA35_03645"
     CDS             9612..10262
                     /locus_tag="VFA35_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZT55295.1"
                     /translation="MKRRQFSIALVGAGAAALTLPTATSAQSSPIDGRNYVRLSTPVP
                     VAAPPGKFEVIEFFWYGCPHCAAFEPVLESWVKQLPEDVAFRRVPVAFRAEPYAAHQR
                     LFYALDSLGLVPALQRKVFYAIHVERQRLDNPGEISAFATKNGIDAAKLLEAYSSFGT
                     QAKLKQANQLVDGYKIDGVPALGIQGRFYTSGTLAGDNERALAVTDFLLQRLRKPG"
     gene            10317..>10539
                     /locus_tag="VFA35_03650"
     CDS             10317..>10539
                     /locus_tag="VFA35_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55296.1"
                     /translation="MTDTLSFSRPRLVHPAFAQVLAGLMLAAALAVPAHAERSDRLQK
                     INIAADQSGQIDLQNQVVAYTGNVVISRGT"
BASE COUNT         1588 a         3660 c         3647 g         1644 t
ORIGIN      
        1 gcgccattga tggtgtagcc gggcaccggc gagaacaccg agtacgcgtc gatcaggatc
       61 cgcgtcttcg cgtcgaagcg ctcacggtct tcgggcgtcc accacaggcg cagcagcccc
      121 gcgccgtcga actggctgcc cttgtcatcg aagccgtggc tgatctcgtg gccgatgacc
      181 gcgccgatcg cgccgtagtt ggcggcgtcg tcggccgcgg cgtcgaagtt cggcgcctgc
      241 aggaacgcgg ccgggaacac gatctcgttg agcgaggcgt cgtagtaggc gttgatggtc
      301 tgcggcgtca tgccccactc gtcgcggtcg atcggcttgc cgagcttggc gagctcgcgc
      361 gcgttctcga acgcgcgtgc gcgcatcacg ttgccgacca ggtcgtcgcg gcgcacctgc
      421 agcgcggcgt agtcgatcca gcgtttcggg tagccgatct tggcattgaa cttcgcgagc
      481 ttggcgagcg cctcgcggcg cgtggccggc gtcatccagt cgagcgtggc gatgctgtcg
      541 cggcaggcgg ccagcaggtt ggcgaccagc tcgtccatgc ggcgtttgct ctcgggcgga
      601 aaatgcttcg ccacgtagag cttgcccagg ccctcgccga tcgactggtc gacgatcgcg
      661 atgccgcgct tccagcgcgg ccggtcctcg agcgtgccgc tcagcacggt gccggcgaac
      721 gcgaaacggg tgtcgacgaa cgccttcgac aggaacggtg cgtaggcgcc gaccaggcgg
      781 acgcgggcat aggccttcca ggtcgcgagc ggcgtcgcgt cgagcagtgc ggccatgccc
      841 gtcaggtagc tgggctggcg caccagcacg tcgggcgtct tgccgaccag gcgggcgctg
      901 tcgaggtacg cgggccagtc gaggccgggg gcaagcgcgg gcagcgcagc gagctcgatg
      961 cggttgtagg ccttgatcgg atcgcggttc tcgaccgcgc tccactgcac gcgcgcgagc
     1021 tcggtctcga gcgcgagcac ggcccgggcc gcggtgccgg cgccgacctt gtcgcccgcc
     1081 agcgtcagca gcttcgcgag gtactcgacg tacttcgcgc gcacgccggc gaacttggca
     1141 tcgtcggtct tcaggtagta gtcgcggttc ggcagcccga ggcccgattg caccagcgag
     1201 ggcacgtagc gggtcgcgtc gcggtcgtcc tggccgacgg acatgccgat cggcatgccg
     1261 gcgcccaggc gcgccaggcg cggcatcagc gcggcgaact ggcgcgtgtc ggccaccgcg
     1321 tcgacaagcg ccagctcggc ggccagcggc gcgaggccga gcttctcgat cgccgcctca
     1381 tccatgaagc tggcgtagaa gtcgccgatc ttcaccgcct cgcggtcgcc gcggcggcgg
     1441 ccggcttcct cgacgagctc gcgcagctgg gcttgcgtcg cctcgtgcat ctgctcggcg
     1501 gcgccgatgt aggccttgtc gggcgggaac acggcgctct tcagccagcg gccgttggcg
     1561 tgggcgtaga ggtcgtcctg cgggcgcacg gacgggtcga tcaggctgcg gtcgatgccg
     1621 ctgccgaggg tgggtggcgg cgcgttctgc gcgctcgcgc cggcggcgac gagcagggtg
     1681 gcggcgagcg ccagcgtcga tctgagcagc aagttcacga agtctcctgg gcgcgccggt
     1741 gggtgcggcg ccggttgcgg cacgggggcc gaatctgcac cgggtaggcg cagcgcgcca
     1801 ctgtagcggc ccgtgccggc accgcacccc cggggtgcga tgaaccgcgg cttcgtgcgg
     1861 atgagctgcg actacgctgc ggccttgacc ttcagccgcc acgcatgcag cagcggctcg
     1921 gtgtagccgc tgggctgcgt caagcccttg aagaccaggt cgtgcgcggc ccggtacgcg
     1981 gcggactcct tgaagcggcc agccatcggc tggtacagcg ggtcgccggc gttttgctgg
     2041 tcgacgacct tcgccatgcg cttgagcgtc gcgttgactt ccttcttgct caccacgccg
     2101 tgcagcatcc agttcgccat gtgctggctg ctgatgcgca aggtggcgcg gtcttccatc
     2161 aggccgacgt tgtggatgtc gggcaccttc gagcagccca cgccctggtc gacccagcgc
     2221 accacgtagc cgaggacgcc ctgcgcgttg ttgtcgagct cctgctggcg ctcggtctcg
     2281 ctccacttcg ccttcttgac gaccggcacg gtcagcaggc cttcgaggat cgccggcgcg
     2341 gcggccgccg catcggtctt ctcgagcgcc ttttgcaccg cgagcacgtc gacctggtgg
     2401 tagtgcagcg cgtgcagcgt cgccgcggtc gggctcggca cccaggcggt gttggcgccg
     2461 gcgcgcggct gcacgatctt ctgttgcagc atcgccgcca tcaggtcggg catcgcccac
     2521 atgcccttgc cgatctgcgc cttgccgcgc agaccgcagc tcaggccgac gagcacgttg
     2581 ctgcgctcgt aggccgcgat ccatgcgctg ctcttcatgt cgcccttgcg gatcatcggc
     2641 ccggcgtgca tcgcggtgtg catctcgtcg ccggtgcggt cgaggaagcc ggtgttgatg
     2701 aacgcgacgc ggtcggccgc cgcggcgatg caggccttca ggttgacgct ggtgcggcgc
     2761 tcctcgtcca tgatgccaag cttgacggtc gccttcggca gcccgagcag ctgctcgacg
     2821 cggccgaaca gctcggccgc gaacgcgacc tcggccggcc cgtgcatctt cggcttgacg
     2881 atgtagaccg agcccttgcg cgagttgacg atgcccgcgg cgcctgccga tccttcgccg
     2941 cgccgcttca ggtcgtgaat cgcgatcgcg gtcgtgatga cggcgtccat gatcccttcc
     3001 ggaatctcct gggcgtcggc gccccacagg atcgccggat tcgtcatcag gtggccgaca
     3061 ttgcgcacga acagcagcga gcggccatgc agccgcacat cctcgccctg cgggccgatg
     3121 tagacgcggt cggggttcag cccgcgcgtg aaggtcttgc cgcccttgct gacctcttcg
     3181 gtcagcgtgc ctttcaggat gccgagccag ttgccgtagg cctgcacctt gtcgtcggcg
     3241 tcgacgaccg cgaccgagtc ctcgaggtcg aggatggtcg acagcgccga ttcgagaatg
     3301 aggtcggaaa cgccggccgg gtcgttcttg ccgatcgcgg tcgagcggtc gatgcgcagg
     3361 tccaggtgca ggccgtggtg ctgcagcagc acggcggacg gcgtggcggc ggcgccctgg
     3421 tagccgacga actgcgcggg cttggccagg ccggtggtcg tgccgtcggc cagcgtgacg
     3481 acgagcgcgc cgccttcgac ccggtagccg gtcgagtcga cgtgcgagcc cttctcgagc
     3541 ggcgcggtac ggtcgagcac gtggcgcgcg tagtcgatga ccttcgcgcc gcgcaccggg
     3601 ttgtagccgg cgcccttttc ggcgccgtcg gcctcggaga tcgcgtcggt gccgtacagc
     3661 gcgtcgtaga gcgaacccca gcgcgcgttg gcggcgttca acgcgtagcg cgcgttggtg
     3721 atcggcacga ccaactgcgg cccggcctgc agcgcaagct cggcatcgac gttcttcgtc
     3781 gtcgccttca cgaccggcgg caccggcacg aggtagccga tcttctgcag gaaggcgcgg
     3841 tacttggcgg ggttcttgat cggcccgggg tgcttcttgt gccaggcgtc gagctcggcc
     3901 tgcaggcgct cgcgctcggc gagcagcgcg gcgttcttcg gcgcgaggtc ggcgacgatc
     3961 gaatcgaagc ccttccagaa cgccgcggcg tcgacaccgg tgccgggcag aacctgctcc
     4021 tcgacgaagc ggtgcagcgc ggtcgcgacc aggaggccgt ggacgggggt tctgtcagtc
     4081 gtgcgtgcgt tcatggtcgt gcgatggcgg attgatcgaa gaaggaaggc acatcgcgca
     4141 aggcgcgtgc cgaggcgcgt ggcacgcgcc attggagaat ttattcgata agccaaccgg
     4201 cattaagtac gatccaatcg ctggattagt gaccaagacg gactgaatca tggacaagct
     4261 gaagcaactc caggccttcg cgtcggtcgc gacccgcggc agcctgaccg ctgcggccgc
     4321 tgccgagggc gttgcgccgg ccgtgatcgg gcggcgcatc gacgcactcg aagcgcgcct
     4381 gggcgtcaag ctgctggtgc gcacgacgcg caggatcagc ctgacgcacg agggcagcgc
     4441 cttcctcgaa gactgccagc gcctgctcgc cgactgggcc aacgccgagg ccagcgtcag
     4501 cgccggcggc gtgaaggcga gcgggcacct gcgcatcacc tcgccggccg gcttcgggcg
     4561 ccgccacgtc gcaccgctgg tgccgcgctt cctggcgctg cacccggagg tcagcctctc
     4621 actgaacctg tcggaccgcg tcgtcgacat cgtcaacgag agcttcgact gcgcggtgcg
     4681 ggtcggcgag ctgcccgaca gcagcctcgt cagcgtgcgc ctggccgaca accggcgcct
     4741 gtgcgtcgcc acacccgggt acctgaagcg cgccggcacg ccgatgcacc cggcggagct
     4801 ggcacgccac gagtgcctgg ccctgagcag cgacgcgagc cagacgcgcg gctgggcctt
     4861 cagcgtcgac ggcggggtga tccgctggcg cccgcgcggg cggctcgact gcaccgacgg
     4921 ccaggtgctg cacgcctggt gctcggccgg gctcggcatc gcgtggcgct cggcctggga
     4981 ggtggagcag gagatcgccg ccggcgaact cgtcagcgtg ctcgacgcct tcgcggcgcc
     5041 gccgaacggc gtctacgcgg tgttcccgca cgccaagcac ctggcgctgc gggtgcggct
     5101 gtggatcgac tttctgaagc acagctatgg cgatccggcc tactgggcac gcagaaactg
     5161 aaaagcccgc cgaagcgggc ctttcaggca agaagcatca gctcacttgc tgaggcattc
     5221 cttcatgaac gccttgcgct cgtcgccctt cgaggccttg gccttggggt cggcgttgca
     5281 ggccttcatc ttttcctgct gggtcgcctt cttggccgac aggcaggtct tcatgaaggc
     5341 cttgcgctcg tcgcccttct tgtcggcagc gtccttgttg cattcgccca tcttggtttg
     5401 ttgcttgccc atcggggcgc tggcggcatc ggcggcgaag gccgaagtcg agagacaagc
     5461 gacggacagg gccagaacgg tcatcagatg tttcatcgga gctcctagag agggttgcat
     5521 gggggcaggt gccggcgagc attgaagcat gcgccccatg cgccagcaag cgcacaacgc
     5581 gccaacgggg cctgcggtgg acacgccacc gtcgtcagag gatgcgccgc gggtgcgcca
     5641 aggcctcgtg cgcggcgtgc agccgacgca ccagcacgcg gatgaacgcc tcgtcgaaca
     5701 ggtgcctgca gcccgggctg acctgcatca gcgtctcggg cgtgaacgag atcgtcgtcg
     5761 ccggctcggt cacgatcacg tcggtgctgt ggcgcttcag ctccgggttc ggcgcgaggt
     5821 agaccatctc gccgatcgag gcgccgctgc cgagctgtgc gaccttcttg ccgtcgcgat
     5881 acacctcgac ctcgccctgc gcgaggatgt ggaagctgtt gccctcctcg cccttgcggt
     5941 acagggcatg accgaagcgg aagcgctgcc acttcgcgcg gtgcaccact tcccagagct
     6001 ccacgtcgcc gaagctcgcg aagaagtcga gcgaacgcag caggttgaag cgctccgagt
     6061 cgagcacgcc ctgcagctgg ccgcgcggca cctgccggct cgtgatcagg cccgacagtg
     6121 cctgcgcgaa ctcctcccag ttggcgtagc gctcggcggg gttcttcgcc agcgcgcgct
     6181 ggatcatcgc gttcagcccg gggctcacgc cttcgcgcag cgtcgccagc gaggccggcg
     6241 ccagatgata tatctggtgc atcatggccg actgcacctg cgcatcgaac ggcgggcgcc
     6301 cggcgatcag gtggtacagc accgcgccga gcgagtacat gtcggcacgc gcatcgagcg
     6361 cgccgccgtc gagctgctcc ggcgacatgt aggcgagcga gccgacgcgg tagatctgcg
     6421 tcgtgtcggc cgagaggttc agcacgctac cgaagtcact gatcttcaca tcggtcacca
     6481 tgccgttgtt cagcaccgcg agcaggttgg ccggcttcac gtcgcggtgg atcaggccct
     6541 ggcgatacac gtaacccagc gccatcgcgc acttgaaccc gatttcgacg atgagttcga
     6601 gcccgagcag ctggtcggcg cggcagtacg gccgcagcgt gctgccggcc acgtactcca
     6661 tcaccaggta cggcgcgacc gggtcggcca ccgcgtcgaa gatctggacc acgttcgggt
     6721 ggttcaggcg gcccaccagc gcagcctcgg cagcgaagaa gcgctcgccg tagcgaccgt
     6781 ccatcgcgtc gccgctgaag ttcggccgca cgcgcttgat cgcgacgtcg atgtccttga
     6841 agtcgtcgtg gcacagaaag acctcggcgg tcgcaccttc gccgagccgc tcgagcacgc
     6901 ggtacttgcc gatgtcgctc ggcaacgccg gcgcgtccgg cggtgggccc ggatcgaagc
     6961 gcatcgggac agtgggcatg gcaatcggca tgggaagctg catcttcgca ccgggccatc
     7021 gcccgcatcg cgacaaagaa ggtgccaaac cgcaactatt cccgcaccgt ggcgctgccg
     7081 atcgtgcccg gtcgggctcg gggtggcccc gtagaatcga cccatgatcc aagccaaaca
     7141 ggacctcctc gcggcgctcg gcgccgcgat caaagaggtc gcgccgcagg cgtcgctcgc
     7201 cccggccttc gagtccccga agcaggccgc gcacggcgac ctcgcctgca ccgcggcgat
     7261 gccgctcgcc aaggccttga agaagaaccc gcgcgaactc gcgagcgcac tggtcgacgc
     7321 gctgcagcgc cagccggctg tgcaggcctg ggtcgatgca gtcgagatcg ccggccccgg
     7381 cttcatcaac ctgcgactga aaccggccgc gaagcaggcg gtcgtcgccg aaatcaacca
     7441 gggcggcgac tgcttcggcc gccagccttc gaagcccgac cggatgctgg tcgagttcgt
     7501 ctcggccaac ccgaccggcc cgctgcacct cggccacgcg cgccaggccg cgctcggcga
     7561 cgcgatctgc aacctgttcg agacgcaggg ctggcaggtc agccgcgagt tctattacaa
     7621 cgacgccggc gtgcagatcg ccacgctcgc gaactcggtc caggcgcggc tgaggggcct
     7681 cgggcccggc gacgcgggct ggcccgagcc ggcctaccac ggcgagtaca tcgccgacat
     7741 cgcggccgac tttttggcga agcaaggcgt caaggccgac gaccgcgaat tcaccgcgtc
     7801 gggcgatgtg gacgatctcg acgccatccg ccagtttgcg gtggcctacc tgcgccacga
     7861 gcaagacctc gacctgcggg ccttcggcgt gcgcttcgat cactactttc tcgaatcgag
     7921 cctgtacgcg tcgggccagg tcgacgccac cgtccagcgc ctgatcgaat ccggcaagac
     7981 cttcgaggac ggcaacgcgc tgtggctgcg caccaccgac tacggcgacg acaaggaccg
     8041 cgtgatgcgc aagcaggacg cgagctacac gtacttcgtg ccggacgtcg cctaccacct
     8101 cgacaagcac gcgcgcggct tcggcaaggc gatcaacatc cagggcggcg accaccacgg
     8161 caccgtcgcg cgggtgcgcg ccgggctgca ggccgcgaac cggggcgtgc cggccggcta
     8221 ccccgagtac gtgctgcaca agatggtcac cgtgatgaag ggcggcgcgg aagtcaagat
     8281 cagcaagcgc gccggcagct acgtgacgct gcgcgacctg atcgattgga ccagccgcga
     8341 cgcggtgcgc ttctttctgc tcagccgcaa ggccgacagc gagttcacct tcgacgtcga
     8401 cctggcgctg cagcgcaacg acgagaaccc ggtgttctac gtgcagtacg cgcatgcgcg
     8461 catctgctcg gtgatccgcc atttcgccga gcagggcggc acgctcggct cgacgcccga
     8521 cctcgcgctg ctgaccgccc cgaccgaagc cgcgctgatg ctcgagctcg ccgagttccc
     8581 ggcgatgctc gcgaatgcgg ccgcgggcct ggcgccgcac gacctgacct tctacctgcg
     8641 cgacgtggcc ggcgcgttcc acagctacta cgcggccgag cgcgtgctgg tcgacgaccc
     8701 ggcgctggcg cgcgcgcgca tcgcgctgct ggtcgcgacg cgccaggtga tccgcaacgc
     8761 gctcgcggtg ctcggcgtca gcgcgcccga gcggatggac cgcgacaccc ctgaatcgaa
     8821 gccggaatcg acatgaacga cagaaagaaa tcccaacgcg gcggctttgc cgtgggtctg
     8881 gtcatcggcc tgctgatcgg cctcgcgctg gcactgggcg tggcgctgta cgtgaccaag
     8941 gtgccgatcc cgttcgtcaa caaggtgccg cagcgcagcg ccgaccagga cgccgccgag
     9001 acccagaaga acaagaactg ggacccgaac agcccgctgt acggcaagaa cccggccaag
     9061 cccaacggtg ccgcgagcgg cacggtcgca ccgctgccgc cggtcgccac gccgcaaccg
     9121 ggtgcgctgc cggcgccggc agtgaccggc agcgtcacgc cggccgcgcc gcgcgcgtcg
     9181 gcgccgtcca gcgcgcgcga cccggcggcg atccttgccg acaagcctgc cgccccgatg
     9241 cccgcgccga agatcaccgc gtcggcgtcg gccgcgggcg gcaccgaccc gttcagctac
     9301 ttcgtccagg ccggcgccta cggccgcacc gaagacgccg aggcgcagcg cgccaagctc
     9361 gcgatgctgg gcatggaggg ccgactcacc gagcgcgaac aggccgggcg caccgtctac
     9421 cgggtgcgcc tggggccgtt cgagaagaag gccgacgccg aagccgcgaa ggaacggctc
     9481 gccggtgccg gcgtcgaggc ggcactggtg cgggtgcagc gctgacgccg cattgggatt
     9541 cgggcctgaa ctttgctgcg cccgatcgac tctgtcacgt ccgcccaccc acacgcaccg
     9601 ccacccttcc catgaaacga cgccagttct cgatcgccct ggtcggcgcc ggcgccgccg
     9661 cgctgaccct gccgactgcc accagcgcgc agagcagccc gatcgatggc cgcaactacg
     9721 tccggctgag cacgccggtg ccggtggccg cgccgccggg caagttcgag gtgatcgagt
     9781 tcttctggta cggctgcccg cattgcgcgg cgttcgagcc ggtgctggaa agctgggtca
     9841 agcagttgcc ggaagatgtc gcgtttcgcc gcgtgccggt cgcgttccgc gccgagccct
     9901 acgcggcgca ccagcgcctg ttctacgcgc tcgacagcct gggcctcgtg ccggccctgc
     9961 aacgcaaggt gttctacgcg atccatgtcg agcgccagcg gctcgacaac ccgggcgaga
    10021 tcagcgcgtt cgcgaccaag aacggcatcg atgcggccaa gctgttggag gcctacagct
    10081 ccttcggcac gcaggccaag ctgaagcagg cgaaccagct cgtcgacggc tacaagatcg
    10141 atggcgtgcc ggcgctcggc atccaggggc gcttctacac ctcgggcacg cttgccggcg
    10201 acaacgagcg cgcgctcgcg gtcaccgact tcctcctgca acgcctgcgc aagcccggct
    10261 gaccccattg gcatgaatca ctgtgggcta gaatggcctg caagtttcat gcctttatga
    10321 ccgacaccct ctccttctcc cggcccaggt tggtgcaccc cgcgtttgcg caggtgctgg
    10381 ccgggctgat gctggccgca gcgctggccg tgccggcgca cgccgaacgc tccgaccggc
    10441 tgcagaagat caacatcgcg gccgaccaga gcggccagat cgacctgcag aaccaggtcg
    10501 tcgcctacac cggcaacgtc gtgatcagcc gcggcacga
//
LOCUS       DASRDG010000221         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_958839, whole genome shotgun sequence.
ACCESSION   DASRDG010000221 DASRDG010000000
VERSION     DASRDG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958839"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFA35_03655"
     CDS             <1..324
                     /locus_tag="VFA35_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZT55297.1"
                     /translation="LGGRLALVCLDRELMERMNRADFDSDGLIDTLRSLDGIEVVAVL
                     KERFDGSVKLSMRSKQDVDVQQIAASFGGGGHRKAAGASVPMSLREAAAAVEERVRAA
                     VEGRA"
     gene            321..1070
                     /locus_tag="VFA35_03660"
     CDS             321..1070
                     /locus_tag="VFA35_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZT55298.1"
                     /translation="MTTFAIISDLHANLEAVEAVLQRIDQLGVRETVCLGDVVGYGAD
                     PGPTTRLVVARCKWTIQGNHDWGLFHSLEEFNPLAREALYFTRRRLKPTVFAPGRRVV
                     WQFLRDLPDRREDHGYLFFHGSPRDPVMEYVLKSDGFLEPEKMQQIFARIDRPCFVGH
                     THWPGVHRGDFRFVSATDEQREFALDGPCVVNVGSVGQPRDGDPRASFAVVAEGKVEI
                     HRVPYDFRRTQAKILAAGLHPALAERLARGK"
     gene            1067..1408
                     /gene="cutA"
                     /locus_tag="VFA35_03665"
     CDS             1067..1408
                     /gene="cutA"
                     /locus_tag="VFA35_03665"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZT55299.1"
                     /translation="MSGDEVVLVLCTAPSQPAPGQRSARELAQALVEARVCACVNVVP
                     GVASVFRWEGRVDHAAEELLLMKTTRAMVPHLRERIAALHPYSVPEMLVFAAVDGLDA
                     YLRWVGEAVAR"
     gene            1405..>2957
                     /locus_tag="VFA35_03670"
     CDS             1405..>2957
                     /locus_tag="VFA35_03670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZT55300.1"
                     /translation="MTGAAPAGRDGRAAQSTGLLQSFLGLLLLFASLAPGNFENEDSG
                     MTMVAARALWRHGDSGLRARDAGAESEAEAYITGLIEGVGDKAPSYGKPGLDGAHRYV
                     WFPMGHVWLMVPFVAAGEALGRAFPQVEERYRALTGPNYVYGSFVFDQGLVALLLPAL
                     CGAATALLLFRIARALGCTPREAVVAAAAVVFATQCFPLAREALSDGPGLCFLLAALW
                     ATVRVCNGDGARRTLLLGGAAAGAAVLTRYPHALLLPPLALAIATALRRRGELRHLLW
                     FALGGLPCALLFAAVNHARYGSFTDTGYPPYASWFNYPLWFGLTKLLIAAGKGILWFS
                     PLLWLLLPLAAQRANVPRLRWLAWTLFLIPLLLFGQTSGWQSGRCWGARYVTPGVVAL
                     LALVLPQCRPWQRWPRGFAALLAAGAFVNVTSLVAPMRGETLLAGQAVMAMYDARHRR
                     GEISDQDWDSVKSEQADHFYFQPRFSPLHANWTYAWRSLTGGFEDELGNLRNGVQYTI
                     EPLFGVTSDV"
BASE COUNT          355 a         1046 c         1101 g          455 t
ORIGIN      
        1 ctcggcggcc ggctcgcgct ggtctgcctc gatcgcgagc tgatggaacg catgaaccgc
       61 gccgacttcg acagcgacgg tttgatcgac acgctgcgct cgctcgacgg catcgaggtc
      121 gtggcggtcc tcaaggagcg cttcgacggc tcggtcaagc tgtcgatgcg cagcaagcag
      181 gacgtcgacg tgcagcagat cgcggcctcc ttcggcggcg gcggccaccg caaggcggcg
      241 ggggccagcg tcccgatgtc gttgcgcgag gctgcggccg cggtcgagga gcgcgtgcgg
      301 gcggcggtcg aggggcgcgc gtgaccacgt tcgcgatcat ctccgacctg cacgcgaacc
      361 tggaagcggt ggaagccgtc ctgcagcgca tcgaccagct cggcgtgcgc gagacggtgt
      421 gcctcggcga cgtggtcggc tacggcgccg atcccggccc gacgacgcgg ctggtcgtcg
      481 cgcgctgcaa gtggacgatc cagggcaacc acgactgggg cctgttccac agcctcgagg
      541 agttcaaccc gctcgcgcgc gaagcgctct acttcacgcg gcgcaggctc aagccgacgg
      601 tgttcgcgcc cgggcgccgc gtcgtctggc agttcctgcg cgatctgccg gaccgccgcg
      661 aggaccacgg ctacctgttc ttccacggct cgccgcgcga tcccgtgatg gagtacgtgc
      721 tgaagagcga cggcttcctc gagccggaga agatgcagca gatcttcgcg cgcatcgacc
      781 ggccctgctt cgtgggccac acgcactggc ccggcgtgca ccggggcgac ttccgcttcg
      841 tctccgcgac cgacgagcag cgcgagttcg cgctcgacgg accgtgcgtc gtcaacgtcg
      901 gcagcgtcgg ccagccgcgc gacggcgatc cgcgcgcgtc gttcgcggtg gtcgcggagg
      961 gcaaggtcga gatccaccgc gtgccgtacg acttccggcg cacgcaggcc aagatcctgg
     1021 cggccggact ccatcccgcg ctcgcggagc ggctcgcgcg cggcaaatga gcggcgacga
     1081 ggtcgtgctc gtgctgtgca cggcgccatc ccagccggcg cccggccagc gctcggcgcg
     1141 cgagctggcg caggcgctgg tcgaagcgcg cgtctgcgcg tgcgtcaacg tcgtgcccgg
     1201 ggttgcgtcg gtgttccggt gggagggccg ggtcgatcat gccgccgagg agctgctgct
     1261 gatgaagacg acccgcgcga tggtgccgca cctgcgcgaa cgcatcgcgg cgctgcatcc
     1321 gtactcggtg cccgagatgc tcgtgttcgc cgccgtcgac gggctcgacg cgtatctgcg
     1381 ctgggtcggc gaggcggtcg cgcgatgacg ggtgcggcgc ccgcgggacg cgacggccgc
     1441 gcggcgcaga gcaccgggct gctgcagtcg ttcctcgggt tgctgctgtt gttcgcgtcg
     1501 ctcgcgccgg gcaacttcga gaacgaggac tcgggcatga cgatggtcgc ggcccgcgcg
     1561 ctgtggcggc acggcgactc gggtctccgc gcgcgcgacg cgggcgccga gtcggaggcg
     1621 gaggcctaca tcacaggcct gatcgagggc gtcggcgaca aggccccgag ctacggcaag
     1681 ccggggctgg acggtgcgca ccgctacgtg tggttcccga tgggccacgt ctggctgatg
     1741 gtgccgttcg tcgccgccgg cgaagcgctc ggccgcgcgt tcccgcaggt cgaggagcgc
     1801 taccgcgcgc tcaccggccc gaactacgtg tacgggtcgt tcgtgttcga ccagggcctg
     1861 gtcgccctgc tgctgccggc gctgtgcggc gccgccacgg ccctgctgct gttccggatc
     1921 gcgcgcgcac tgggctgcac gccgcgcgag gcggtggtcg ccgcggccgc ggtcgtgttc
     1981 gcgacgcagt gcttcccgct ggcgcgcgag gcgctcagcg acggccccgg gctgtgcttc
     2041 ctgctcgccg cgctgtgggc gaccgtgcgc gtctgcaacg gcgacggcgc acgccgcacc
     2101 ctgctgctcg gcggcgcggc ggccggcgcc gcggtgctga cgcgctaccc gcacgcgctg
     2161 ctgctgccgc cgctcgcgct ggcgatcgcc acagcgctgc gccgccgcgg cgaactgcgg
     2221 cacctgctgt ggttcgcgct cggcggcctg ccgtgcgcgc tgctgttcgc ggccgtcaac
     2281 cacgcgcgct acggcagctt caccgacacc ggctatccgc cgtacgcgag ctggttcaac
     2341 tacccgctct ggttcgggct caccaagctg ctgatcgccg cgggcaaggg catcctgtgg
     2401 ttctcgccgc tgctgtggct gctgctgccg ctggccgctc agcgcgcgaa cgtgccgcgg
     2461 ctgcgctggc tcgcgtggac gctgttcctg atcccgctgc tgctgttcgg ccagacgagc
     2521 ggctggcagt cggggcgttg ctggggcgcg cgctacgtga cgccgggcgt cgtcgccctg
     2581 ctggcgctcg tgttgccgca gtgccggccg tggcagcgct ggccgcgcgg gttcgcggcg
     2641 ctgctcgccg ccggcgcgtt cgtgaacgtc acgtcgctgg tcgcgccgat gcgcggcgag
     2701 acgctgctcg cggggcaggc ggtcatggcg atgtacgacg cgcgccaccg gcgcggcgag
     2761 atcagcgacc aggactggga cagtgtgaag agcgaacagg ccgaccactt ctatttccag
     2821 ccgcgcttct cgccgctgca cgccaactgg acctacgcgt ggcgctcgct caccggcggc
     2881 ttcgaggacg aactcggcaa cctgcgcaac ggcgtgcagt acaccatcga gccgctgttc
     2941 ggcgtcacga gcgacgt
//
LOCUS       DASRDG010000222         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1184676, whole genome shotgun sequence.
ACCESSION   DASRDG010000222 DASRDG010000000
VERSION     DASRDG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184676"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1893)
                     /locus_tag="VFA35_03675"
     CDS             complement(<1..1893)
                     /locus_tag="VFA35_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZT55301.1"
                     /translation="MNTAPSTQHLAAQPVAEDDGPLSPWWLRAVAIVMVLGFAGLILI
                     TTLSYRNAPPIPARTVDPQGVALFSAEDVGEGQAVFLRYGLMANGSIWGHGAYLGPDF
                     SATALHRMGMVAAESISSEQYHQAFGTLNESQAAAVQAEAIVSLKKNRYDESTGTLQF
                     SGAQAKAFNADIAHWAAYFHDPARNAGLKADLITDPVELRQFTAFVTWAAWASVAARP
                     GEAHSYTNNFPYDPSVGNVPISGALLWSALSLMVLLAGISTVLLAFGKFDYLGWITRG
                     HNVKPQMLPGRASRGQQALVKFFVVVSVLLLAQALVGGAVAHYRADPGSFYGFQLERI
                     FPSNLMRTWHLQLAIFWIATAYVAAALFLGRSLRTDEPSWLAGWTHVLFTAFAVVIFG
                     SLLGEWAGIAQLLGSWWFWLGNQGWEYLELGRVWQVLLVVGLFVWFALLWSLVRPRAL
                     ADRASQPIVRMFLVAALAIPVFYIPAMFFGAKTNYTVVDTWRFWIIHLWVEGFFEFFA
                     TTIVALTFYQLGLTRRNVALRVIYLDAILYFLGGLMGTGHHWYFTGQTNVNMALSALF
                     SALEVVPLTLLTLDAWDFVRTTRGDRDAAGKQMVVPHKWTFYFLMAVGFWNFVGAGIF
                     GFLINLP"
     gene            complement(1890..2585)
                     /locus_tag="VFA35_03680"
     CDS             complement(1890..2585)
                     /locus_tag="VFA35_03680"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZT55302.1"
                     /translation="MKFSIYRFDPEQDRKPYMQDFDEPLNDGDRMLLDVILRIKAQDN
                     TLTIRKSCREGVCGSDAININGRNGLACITRVWDLKEPVVLRPLPSYPVIRDLVVDMS
                     RFYAQYHSIKPYLVNNEPPPEKERLQSPQERERLNGLYECILCGCCTSQCPSSWWNPE
                     KFVGPAGLLQADRFIADSRDRATHERLDDLNDVYRLFRCRSIMNCTEVCPKGLAPSRA
                     IESIRLTMLKEAL"
     gene            complement(2636..>2834)
                     /gene="sdhD"
                     /locus_tag="VFA35_03685"
     CDS             complement(2636..>2834)
                     /gene="sdhD"
                     /locus_tag="VFA35_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HZT55303.1"
                     /translation="GHIGVSAASGVFIAALLAHAWVGLRDVTLDYVAPLAARVAVLSL
                     IAATLVAVAAFAFRILFLAHG"
BASE COUNT          562 a          895 c          923 g          454 t
ORIGIN      
        1 cggcaggttg atcaggaaac cgaagatgcc cgcgccgacg aagttccaga agcccaccgc
       61 catcaggaag tagaaggtcc atttgtgcgg gacgaccatc tgcttgcccg cagcatcacg
      121 gtcgccgcgc gtggtgcgca cgaagtccca cgcgtcgagc gtcagcaagg tcagcggcac
      181 cacctccagt gccgagaaca gcgctgacag cgccatgttg acgttggtct ggccggtgaa
      241 gtaccagtga tgacccgtgc ccatcagccc gccgagaaag tacaggatcg cgtcgaggta
      301 gatcacgcgc agtgcgacgt tgcggcgggt gaggccgagt tggtagaacg tcagcgccac
      361 gatcgtggtc gcgaagaact cgaagaagcc ttcgacccac agatggatga tccagaagcg
      421 ccaggtgtcg accaccgtgt agttggtctt cgcgccgaag aacatcgccg ggatgtagaa
      481 caccgggatc gcgagcgccg ccaccaggaa catcctgacg atcggctgcg aggcgcggtc
      541 ggccagcgcg cgcggacgaa ccagcgacca cagcagcgcg aaccagacga acagccccac
      601 gaccagcagc acttgccaga cacgcccgag ttcgaggtat tcccaaccct ggttgccgag
      661 ccagaaccac cacgacccga gcagttgcgc aatgcccgcc cactcgccca gcaggctgcc
      721 gaagatgacc accgcgaacg ctgtgaagag aacgtgcgtc caacccgcga gccaactcgg
      781 ctcgtcggtt cgcagcgagc ggccgaggaa cagtgcggcg gccacgtaag cggtcgcgat
      841 ccagaagatc gccaactgca gatgccaggt gcgcatcaag ttgctgggga agatgcgttc
      901 gagctgaaaa ccgtagaagc tgcccggatc ggcacggtag tgcgcgaccg cgccgccgac
      961 cagcgcctga gccagcagca gcactgacac gaccacgaag aacttcacga gtgcctgttg
     1021 cccacggctg gcccgccccg gcaacatctg cggcttgacg ttgtggccgc gcgtgatcca
     1081 gcccaggtag tcgaacttgc cgaaggccag cagcacggtc gatattcctg cgagcagcac
     1141 catcaggctg agcgcgctcc acaggagcgc acccgagatg ggcacgttgc cgacgcttgg
     1201 atcgtacgga aagttgttgg tgtaggagtg cgcttcgccg ggcctggcgg ccacggaggc
     1261 ccaggcggcc caggtcacga aagctgtgaa ttgccgaagc tcgaccggat cggtgatcag
     1321 gtcggccttc aggcccgcgt tgcgcgccgg gtcgtgaaag taggcggccc agtgcgcgat
     1381 atcggcgttg aacgccttcg cttgcgcgcc gctgaactgc agcgtgccgg tggactcgtc
     1441 gtagcgattc ttcttcagcg acacgatcgc ctctgcctga acggcggcgg cctggctttc
     1501 gttcagcgtt ccgaaagcct ggtgatactg ctcgctcgag atgctttctg cggcgaccat
     1561 ccccatccga tgcagtgcgg tggccgagaa atcgggcccg aggtacgcgc cgtggcccca
     1621 gatgctgccg ttggccatca ggccgtagcg caggaacacc gcctgccctt cgccgacatc
     1681 ttctgcgctg aacagcgcga cgccttgtgg gtccacggtc cgcgccggaa tcggcggcgc
     1741 attgcgataa gaaagcgtgg tgatcaggat caaccccgca aaaccgagca ccatcacgat
     1801 cgccacggcc cgcagccacc acggtgacag tgggccgtcg tcttctgcga ccggttgcgc
     1861 agccaggtgc tgcgtcgacg gcgcggtgtt cacagtgcct ccttcagcat ggtcagacgg
     1921 atgctctcga tcgcccgcga cggcgcaagc cccttggggc agacctccgt gcaattcatg
     1981 atcgagcggc agcggaacaa gcggtagacg tcgttcaggt catcgagccg ctcgtgcgtg
     2041 gcacggtcgc ggctgtcggc gatgaagcgg tctgcctgca agaggccggc cggccccacg
     2101 aatttttcgg ggttccacca cgacgacggg cactggctgg tgcagcaacc gcacaggatg
     2161 cactcgtaca ggccgttcag tcgctcgcgt tcttgcggcg actgcaagcg ctctttctcg
     2221 ggcggcggtt cgttgttgac tagatagggc ttgatggagt ggtactgcgc atagaagcgg
     2281 gacatgtcga caacgagatc gcggatcacc ggataactcg gcagcgggcg cagcaccacc
     2341 ggctccttca gatcccagac ccgggtgatg caggccaggc cgttgcggcc gttgatgttg
     2401 atcgcgtcag agccacaaac gccctcacgg caagacttgc ggatcgtcag cgtgttgtcc
     2461 tgtgccttga tccgcagaat cacgtccagc agcattcggt cgccgtcgtt gagaggttcg
     2521 tcgaagtcct gcatgtaggg cttgcggtct tgctccgggt cgaagcgata gatggaaaac
     2581 ttcatgcgag cctttcaggg cgatcgaagg tgtcgcgggc gaagttcacc tgcgttcacc
     2641 cgtgagcgag aaacagaatc cgaaaggcga atgcggccac agcgaccagc gttgcggcaa
     2701 tcagcgacag aacggcaacg cgtgcggcga gcggggcaac gtagtcgagc gtgacgtcac
     2761 gcaggccgac ccaggcgtgc gccaggagcg ctgcgatgaa cacgccggac gccgcgctga
     2821 caccgatgtg gccc
//
LOCUS       DASRDG010000223         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1417059, whole genome shotgun sequence.
ACCESSION   DASRDG010000223 DASRDG010000000
VERSION     DASRDG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417059"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /locus_tag="VFA35_03690"
     CDS             complement(<1..91)
                     /locus_tag="VFA35_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZT55304.1"
                     /translation="MKTDMAINALAALAQESRLAIFRYLVEAGP"
     gene            331..>3087
                     /locus_tag="VFA35_03695"
     CDS             331..>3087
                     /locus_tag="VFA35_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55305.1"
                     /translation="MKSICTTPSRTRRLPCLPPASALAALLAVFALPATANTFTVTTG
                     TDGSQQVGNTATCVEVNGTNTPTGNGCSLRAAIMLGNNITGPHTINFNPAVTMVTVVN
                     GAMAQMRAQYTVTGAFPLRTAINGNGHGCLDLTDSGTEQASPAIHNGKGATGSTISNL
                     VIGNCSGAGISANGHGYTFNNNYIGVDPTGLAAWPNGGNGVWLSASIAYDDQFVDTTA
                     LGNLNSALPVQPASAVDISNFSTGLAGVLVNLQPNTIKDNVISGNTLNGIELFSKTLA
                     ATLMSNNMIGTNSTGTLPIANGQSGVHLVGSTFGNLIGPNNVIAGNTQNGIQVDAGAV
                     YLPNFIMGNRIGLSNVNGAHVGNMQSGIVVDTKPSSDPAYFNPSLTGLVIGPLNVIAD
                     NQGTNNALNLDDQNDTQGGILITGASTGIKVLGNTIGIAEFPAGTPLASKAYANLGDG
                     IIVTTSGNQIGGSAATAGNIVAANARHGIVVHGIATTGNSFAGNSVGVHPGLGGNLAL
                     GNGVDGFHIDNASATTIGGSGSSDFNTIAANGRNGVKIVNGNAQNGWGNLLQRNRIYH
                     NAVAMAGVGIDLDRAQNASNPLITLPPEIPANYTNLDQAQPVICTGPGDAGACAGSAA
                     PLSNGSSVTLQWTITTHGPNASLRMEFFALDKADANLAGQLAWFDEQTVITDANGKPT
                     GANCSNGRCSMTLTGALSGTWLAMTATDITALTNVAGAPGDWKAGLKCFIGQFGDPTG
                     IISACPANNTSEFSNVANVPAMAPSVTTNAASAITTTTATLNGLVNDNGATTALAFEY
                     GATANYGTPATAVPASLAAGAGSTAITGPISGLTCGTTYHFRINASNGIGGISFGNDM
                     SFTTSACPAQAPTATTTAATMITASGAQLNGTVNDNGATTAISFEYGPDTNYGTLVSA
                     VPASL"
BASE COUNT          620 a         1109 c          897 g          461 t
ORIGIN      
        1 ccggcccggc ttccacgagg tagcgaaaaa ttgccaggcg actttcctgg gcgagcgcag
       61 ccaatgcgtt gatggccata tctgttttca tatttccaat aatatcgaaa tatcatgctc
      121 cgtcaagcaa ctcgaatcga aactccgatg gcttccagca gatgagcgat tcgccagccg
      181 catgcgcgca ggtgcaaccg ttgcaagcgg ctgggtcgac cggttagcca cggactgacc
      241 gacccggcgc ggccaaccca ttgccggggc cgtgatccgt ttttccggtt ttccgcgtcc
      301 tgctacgcga tcgcaaaccg gaaaatcgcc atgaagtcca tctgcactac gccctcgcgc
      361 acgcgccgtc tgccgtgcct cccaccggca tcggccctcg ccgcattgct tgccgtcttc
      421 gcgctgcccg ccacggcgaa tacgttcacc gtcaccaccg gcaccgacgg ctcgcagcag
      481 gtcggcaaca cggcgacctg cgtcgaggtc aacggcacga atacgccgac cggcaacggc
      541 tgcagcctgc gcgcggcgat catgctcggc aacaacatca ccgggccaca cacgatcaac
      601 ttcaatcccg cggtgacgat ggtgaccgtg gtcaacggcg ccatggcgca gatgcgcgcg
      661 caatacacgg tcaccggcgc gttcccgctg cgcaccgcga tcaacggcaa cggccacggc
      721 tgccttgatt tgaccgattc gggcaccgag caggcttccc cggcgatcca caacggcaag
      781 ggcgcgaccg gatcaacgat cagcaacctc gtgatcggca actgcagcgg tgcgggcatc
      841 agcgcgaacg ggcatggcta tacgttcaac aacaactaca tcggtgtgga tccgaccggt
      901 ctcgccgcct ggccgaatgg cggcaacggc gtctggctgt ccgcgtcgat cgcctacgac
      961 gaccagttcg tcgataccac ggcgttgggc aatctcaaca gcgcactgcc ggtgcagccg
     1021 gccagcgccg tcgatatcag caatttctcc accggtctcg ccggcgtgct ggtgaacctg
     1081 cagccaaaca cgatcaagga caacgtcatc tcgggcaaca cgctcaacgg catcgagctg
     1141 ttcagcaaga ccctggccgc gacgctgatg tcgaacaaca tgatcggcac caactcgacc
     1201 ggtactctgc cgatcgcgaa cggccagagc ggcgtgcatc tggtcggcag cacgttcggc
     1261 aacctgatcg gcccgaacaa cgtcatcgcc ggcaatacgc agaacggcat ccaggtcgac
     1321 gccggtgcgg tctacctgcc gaacttcatc atgggcaacc gcatcggcct gtccaacgtc
     1381 aatggcgcgc acgtcggcaa catgcagagc ggcatcgtcg tcgacaccaa gccttcgtcc
     1441 gacccagcct acttcaaccc gagcctgacc gggctcgtga tcggcccgct caacgtgatc
     1501 gccgacaacc agggcacgaa caacgcgctc aaccttgacg accagaacga cacccagggc
     1561 ggcatcctca tcaccggtgc gagcaccggc atcaaggtgc tcggcaacac gatcggcatc
     1621 gccgagttcc cggccggcac gcctctggca tcgaaggcct acgccaatct cggcgacggc
     1681 attatcgtca ccacctcggg caaccagatc ggcggcagcg ctgcaaccgc cggcaacatc
     1741 gtcgcggcca atgcgcgcca cggcatcgtc gtccacggca tcgcgacaac cggcaacagc
     1801 ttcgccggca actcggtcgg cgtgcatccg ggcctcggcg gcaatctcgc gctcggcaac
     1861 ggcgtcgacg gtttccacat cgacaacgcc agcgcgacca cgatcggcgg cagcggctcg
     1921 agcgatttca acacgatcgc cgcgaacggc cgcaacggcg tcaagatcgt caacggcaac
     1981 gcgcagaacg gctggggcaa cctgctgcag cgcaatcgca tctaccacaa cgcggtcgcc
     2041 atggccggcg tcggcatcga tctcgatcgc gcgcagaacg caagcaatcc gctcattacc
     2101 ctgccgcctg agattccggc gaactacacc aacctcgacc aggcgcagcc ggtgatctgc
     2161 accggccccg gcgatgcggg cgcgtgcgcg ggctccgctg cgccgctgtc gaacggcagt
     2221 tcggtgacgt tgcagtggac catcaccacg cacggcccca acgcgagcct gcgcatggaa
     2281 ttcttcgctc tcgacaaggc cgacgcgaac ctggcgggac aactcgcctg gttcgatgaa
     2341 cagaccgtca tcaccgatgc gaacggcaag cccaccggtg ccaattgctc gaacggacgc
     2401 tgcagcatga ccctcacggg cgcgctcagc ggcacctggc tggcgatgac ggcgaccgac
     2461 atcaccgcgc tgaccaatgt ggccggcgcg cccggcgact ggaaggccgg tctcaagtgc
     2521 ttcatcggcc agttcggcga cccgaccggt atcatctccg cctgtccggc gaacaacacc
     2581 tccgaattct ccaatgtcgc caacgtgccg gcgatggcgc cgagcgtgac gacgaacgcg
     2641 gcgagcgcga tcacgacgac tacggcgacg ctcaacggcc tcgtcaacga caacggtgcg
     2701 accaccgcgc tcgccttcga gtacggcgcg accgcgaact acggcacgcc ggccacggcg
     2761 gtgcccgcct cgctggctgc cggcgcgggc agtactgcga tcaccgggcc catcagcgga
     2821 ctgacctgtg gcacgacgta tcacttccgc atcaacgcga gcaacggcat cggcggcatc
     2881 tccttcggca acgacatgag cttcacgact tcggcctgtc ccgcgcaggc tccgactgcc
     2941 acgacgaccg cagcgaccat gatcaccgca agcggggccc agctcaacgg cacggtcaac
     3001 gacaatggcg cgaccaccgc gatcagcttc gagtacggtc cagacacgaa ttacggcacg
     3061 ctggtcagcg ccgttcccgc ttcgctg
//
LOCUS       DASRDG010000224         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_605791, whole genome shotgun sequence.
ACCESSION   DASRDG010000224 DASRDG010000000
VERSION     DASRDG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605791"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFA35_03700"
     CDS             complement(<1..228)
                     /locus_tag="VFA35_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HZT55306.1"
                     /translation="MVGILGIFVSLALLIYLAHRGWNVIFIAPVCAAVAILFNWGDLP
                     LMASYTQIFMPALGTYFATYFPLFLLGAVFGK"
     gene            454..1920
                     /locus_tag="VFA35_03705"
     CDS             454..1920
                     /locus_tag="VFA35_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZT55307.1"
                     /translation="MPHCAPPATRPPADDWPGAVLGHGADTIRDIAMRSLFDHLEGLC
                     EGAVAVDMDGRIVWIHEKYARKAGLACAAAALGREIEEVIPNSQLREVATSGVPIVLD
                     IMALAGEHLVVTRMPLRDADGRPVGAIGFVLYNRVEGLKPLIAKVTQLQARLKAAEQE
                     LADNRRAKYSLASFLGMSAAAIEIKRQARRAAQLDATVLLLGETGTGKELLAHAIHNV
                     SPRAQRPFISVNAAAIPEPLLEAELFGAAPGAYTGADRRGRDGKFKLADGGTLFLDEV
                     GDMPLALQAKLLRVLQEHEIEPLGANKLLRLDVRVIAATSVDLEARVADGRFRADLYY
                     RLNVLTLALPPLRERLDDLGLLVERLLDDIGLRLGHPIEIDAAALQVLAQHAWPGNVR
                     ELGNVLERALLRHETQRLTADQIASVLPAAVLALPAAASAETSAAVEPLAMAVATAER
                     RSIGAALRATGGNKVAAAQALGISRAALYQKLAALGLR"
     gene            complement(1930..>2094)
                     /locus_tag="VFA35_03710"
     CDS             complement(1930..>2094)
                     /locus_tag="VFA35_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HZT55308.1"
                     /translation="KQAFELLAAKRGSAHAAQHEVMECLGEMVWASQRSGAPPDGARY
                     LECVRRRATR"
BASE COUNT          319 a          741 c          747 g          287 t
ORIGIN      
        1 cttgccgaac accgcgccga gcaggaacag cgggaagtag gtcgcgaaat acgtgcccag
       61 cgccggcatg aagatctgcg tgtagctcgc catcaacggc aggtcgcccc agttgaacag
      121 gatcgcgacg gcggcgcaga ccggcgcgat gaagatgacg ttccagccgc ggtgcgcgag
      181 atagatcagc agcgcgagcg agacgaagat gccgagaatg ccgaccatga aacgagactc
      241 ctgaagcgcc gagggatggg ccggtattgc gaggcgcgtg ccagcgcgcg cagcgcgccg
      301 attcgtcgtg gttaacccgc gattccaggc cgattcggcg tgattcgggg tgcggtcgac
      361 gccgccgttg tctgcgcgcc ggcacagcgc cgtcaagctg cgcgtacagt tggcgcgcag
      421 acggtgacca gccgcatttc aggagacaag cccatgcccc actgcgcgcc gcccgccacg
      481 cgcccgcccg ccgacgactg gcccggtgcc gtgctcggcc acggcgccga cacgatccgc
      541 gacatcgcga tgcgctcgct gttcgaccat ctcgagggcc tgtgcgaagg tgccgtcgcg
      601 gtcgacatgg acggccgcat cgtctggatc cacgagaagt acgcgcgcaa ggccggcctg
      661 gcctgcgcgg ccgcggcgct gggccgcgag atcgaagagg tcatcccgaa cagccagctg
      721 cgcgaggtcg cgacgagcgg cgtgccgatc gtgctcgaca tcatggcgct cgctggcgag
      781 cacctggtcg tgacgcgcat gccgctgcgc gacgccgatg gccggccggt cggcgcgatc
      841 ggcttcgtgc tctacaaccg cgtcgagggg ctgaagccgc tgatcgccaa ggtcacgcag
      901 ttgcaggcgc gcctcaaggc cgccgagcag gagctggccg acaaccggcg cgcgaagtac
      961 agcctcgcgt cgtttctcgg catgtcggcc gccgcgatcg agatcaagcg ccaggcgcgc
     1021 cgcgccgcgc agctcgacgc gacggtgctg ctgctcggcg agaccggcac cggcaaggag
     1081 ctgctcgcgc acgcgatcca caacgtctcg ccgcgcgcgc agcgaccgtt catcagcgtc
     1141 aacgccgcgg cgattcccga gccgctgctc gaagccgaac tgttcggtgc cgcgcccggc
     1201 gcctacacgg gcgccgatcg gcgcggccgc gacggcaagt tcaagctcgc cgacggtggc
     1261 acgctgttcc tcgacgaggt cggcgacatg ccgctggcgc tgcaagccaa attgttgcgg
     1321 gtgttgcagg agcacgagat cgagccgctc ggcgccaaca agctgctgcg cctcgatgtg
     1381 cgcgtgatcg ccgcgaccag tgtcgatctc gaggcgcgcg tcgccgacgg gcgctttcgt
     1441 gccgacctgt actaccggct caacgtgctg acgctcgcgc tgccgccgct gcgcgagcgg
     1501 ctcgacgacc tgggcctgct cgtcgagcgg ctgctcgacg acatcggcct gcgcctgggc
     1561 cacccgatcg agatcgatgc cgcggcgctg caggtgctgg cgcagcacgc ctggcccggt
     1621 aacgtgcgcg aactcggcaa cgtgctcgaa cgcgcgttgc tgcgccacga gacgcaacgg
     1681 ctgacggccg atcagatcgc gagcgtgctg ccggcggctg tgctggcgct gccggcggcg
     1741 gccagcgccg agacatcggc tgcggtcgag ccgcttgcga tggcggtcgc cacggccgag
     1801 cgccggtcga tcggcgcggc gctgcgcgcg accggcggca acaaggtcgc cgcggcgcaa
     1861 gcgctgggca tctcgcgcgc cgcgctgtat cagaagctgg ccgcgctcgg cctgcgctga
     1921 gctggccgat cagcgcgtcg cgcgccgccg cacgcattcg aggtagcgcg cgccgtcggg
     1981 gggcgcgccg ctgcgctgcg aggcccagac catctcgccc aggcactcca tcacctcgtg
     2041 ctgcgccgcg tgcgccgagc ctcgcttcgc ggcgagcagc tcgaatgcct gctt
//
LOCUS       DASRDG010000225         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_605898, whole genome shotgun sequence.
ACCESSION   DASRDG010000225 DASRDG010000000
VERSION     DASRDG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605898"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1943
                     /locus_tag="VFA35_03715"
     CDS             <1..1943
                     /locus_tag="VFA35_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HZT55309.1"
                     /translation="YLIAYRFYSKFIAAKVFALDAERATPATRLSDGRDFVPTNKWVV
                     FGHHFAAIAGPGPLVGPTLAAQFGFLPGALWIIVGVAVGGAVQDLVILCASIRRDGRS
                     LGQMARDEIGPVGGVTALLAVFGIMIVLIAVLGLVVVNALAQSPWGTVTIGLTIPIAL
                     LMGAYLHWLRPGRVLEATAIGLLLLVLALYAGKWVSESATLAPMFTWEKRTLAFCIMG
                     YGFAASVTPVWMLLAPRDYLSAFVKIGVVLALALGILVVLPPLHMPAVTRFVDGTGPV
                     FAGRLFPFLFVTIACGAISGFHSLVASGTTPKLIARETDARMIGYGSMLTESLVATLA
                     LIAACVLTPGMYFAINAPPSALGTTAESAAQAIQQWGFTIAPGDIDALAHQVGETTLL
                     SRTGGAPSLAVGMANIFAQALGGSGALALWYHFAIMFEALFILTTLDAGTRVGRFLLQ
                     DLGKHVWAPFGRVSWYPAVVLASALFVAIWGWFLYQGVVDPYGGIKALWPLFGISNQL
                     LAAVALCVGTTVIVKMGKARFAPLTIAPLLWLMTVNLTAGWQLVFSKAAPVGFVPHAQ
                     ERSAQLDAGTLPAGVATAGELRRLIFNDWLDACVAGCFMLAAVVILLASAREWWLVAS
                     GRKPAVSSEVPFAARPAGALAE"
     gene            1940..2155
                     /locus_tag="VFA35_03720"
     CDS             1940..2155
                     /locus_tag="VFA35_03720"
                     /inference="COORDINATES: protein motif:HMM:NF016234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HZT55310.1"
                     /translation="MNAPGSNALSRIAAQLRRLAAALRRIAGAPDYDAYRAHMRRHHP
                     TAAPLSRDAFEKEQWQRRASRPGNRCC"
     gene            complement(2238..2522)
                     /locus_tag="VFA35_03725"
     CDS             complement(2238..2522)
                     /locus_tag="VFA35_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZT55311.1"
                     /translation="MARTLDIVGERWTLLVLRDLFLHGPRRYQDLQESLAGVAPNTLS
                     ARLKALEEHGFVERKLYNEHPPRLEYHLTEKGRSFGPVMKALRDWGQRNT"
     gene            2634..3257
                     /locus_tag="VFA35_03730"
     CDS             2634..3257
                     /locus_tag="VFA35_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55312.1"
                     /translation="MDARQFTRWALAAWFVAVLAVGAAGGFVTSPNVPPLPIVAGVAL
                     PLAAFVTAYSRWGGFRSLVLSADLRLLTGIQAWRWAGLGFLFLYAYGVLPGLFALPAG
                     LGDMAIGLTAPWMVARLASDPAFAASRRFAVWNALGILDLAVAVGLGGAASGIIPGLT
                     SVLTTPMARLPLILIPAFLVPFFVMLHLTAFFQARRASAVGYGAVPA"
BASE COUNT          374 a         1182 c         1167 g          570 t
ORIGIN      
        1 tctacctgat cgcgtaccgc ttctactcga agttcatcgc ggcgaaggtc ttcgcgctgg
       61 acgccgagcg ggcgacgccg gccacgcgcc tgtccgacgg gcgtgatttt gtgcccacga
      121 acaagtgggt ggtgttcggg caccacttcg cggccatcgc ggggccgggg ccgctggtcg
      181 gcccgacgct cgcggcgcag ttcggcttcc tccccggcgc gctgtggatc atcgtcggcg
      241 tggcggtcgg cggcgccgtg caggacctcg tgatcctgtg cgcgtcgatc cggcgcgacg
      301 gccgctcgct cgggcagatg gcgcgcgacg agatcgggcc ggtcggcggc gtcaccgcgc
      361 tgctcgcagt gttcggcatc atgatcgtgc tgatcgcggt gctcgggctg gtcgtggtca
      421 acgcgctggc gcagagcccg tggggcacgg tcaccatcgg gctcacgatc ccgatcgcgc
      481 tgctgatggg cgcgtacctg cactggctgc ggcccgggcg cgtgctggag gcgaccgcga
      541 tcgggcttct gctgctggtg ctcgcgctct acgcgggcaa gtgggtctcg gagagcgcga
      601 cgctcgcgcc gatgttcacg tgggagaagc ggacgctcgc gttctgcatc atgggctacg
      661 gcttcgcggc gtcggtgacg ccggtgtgga tgctgctcgc gccgcgcgac tacctgagcg
      721 cgttcgtgaa gatcggcgtc gtgctggcgc tggcgctcgg catcctggtg gtgctgccgc
      781 cgctgcacat gccggcggtg acgcggttcg tcgacggcac cgggccggtc ttcgcgggcc
      841 ggctgttccc gttcctgttc gtgacgatcg cgtgcggcgc catctcgggc ttccattcgc
      901 tggtcgcgtc cggcaccacg ccgaagctga tcgcccgcga gaccgacgcg cgcatgatcg
      961 gctacggcag catgctcacc gagtcgctgg tcgccacgct ggcgctgatc gccgcgtgcg
     1021 tgctgacgcc ggggatgtac ttcgcgatca acgcgccgcc gagcgcgctc ggcaccaccg
     1081 ccgagagcgc ggcgcaggcg atccagcagt ggggcttcac gatcgcgccc ggcgacatcg
     1141 atgcgctggc gcaccaggtc ggcgagacca cgctgctgtc gcgcaccggc ggcgcgccgt
     1201 cgctcgcggt cggcatggcg aacatcttcg cgcaggcgct cggcgggtcc ggcgcactcg
     1261 cgctctggta ccacttcgcg atcatgttcg aggcgctgtt catcctgacg acgctcgacg
     1321 ccgggacgcg cgtcgggcgc ttcctgctgc aggacctggg caaacacgtg tgggcgccgt
     1381 tcggccgcgt gtcgtggtat cccgccgtcg tgctcgccag cgcgctgttc gtcgccatct
     1441 ggggctggtt cctctaccag ggcgtggtcg acccgtacgg cggcatcaag gcgctgtggc
     1501 cgctgttcgg gatctccaac cagctgctcg ccgcggtcgc gctgtgcgtc ggcacgacgg
     1561 tgatcgtgaa gatgggcaag gcgcgtttcg cgccgctgac gatcgcgccg ttgctctggc
     1621 tgatgacggt caacctcacc gccggctggc agctcgtgtt ctccaaggcg gcgccggtcg
     1681 gtttcgtgcc gcacgcgcag gagcgaagcg cacagctcga cgccggcacg ctgcccgccg
     1741 gcgtggcgac ggccggcgag ctgcggcgcc tgatcttcaa cgactggctc gacgcctgcg
     1801 tcgccggctg cttcatgctc gccgcggtcg tgatcctgct ggcctcggcg cgcgaatggt
     1861 ggctcgtcgc cagcggccgc aagccggccg tgtcgtccga ggtgccgttc gcggcgcggc
     1921 cggcgggagc gctcgccgaa tgaacgcgcc cggcagcaac gcgctctcgc gcatcgccgc
     1981 gcagctgcgc cgactggccg cggcgctgcg ccgcatcgcc ggcgcgccgg actacgacgc
     2041 gtaccgcgcg cacatgcggc ggcatcatcc gacggcggcg ccgctgtcgc gcgacgcctt
     2101 cgagaaggag cagtggcagc gccgcgcctc gcggccgggc aaccgctgct gctgatcgcg
     2161 ctcctgatcg acacgccggc gacgacactc gacgtcggcc cgcgcgcctc gtgtcgcgct
     2221 ggccagccag tcgtcgctca ggtgttgcgc tggccccagt cgcgcagcgc cttcatcacc
     2281 ggcccgaagc tgcggccctt ctccgtcagg tggtactcga ggcgcggcgg gtgctcgttg
     2341 tagagcttgc gctcgacgaa gccgtgctct tccagcgcct tcaggcgcgc ggagagcgtg
     2401 ttcggtgcga cgccggcgag cgactcctgc aggtcctggt accggcgcgg gccgtgcagg
     2461 aacaggtcgc gcaggacgag cagcgtccag cgctcgccga cgatgtcgag cgtgcgcgcc
     2521 acggggcagc gcttctggcg gtaggagcgg gacacgcggc aaagataccc cattgactcc
     2581 attccaacaa gtgactacag aaaacatagt aactatcctt cgaggagacg ctcatggacg
     2641 ctaggcaatt cactcgctgg gcgctcgccg cctggttcgt cgcagttctc gccgtcggcg
     2701 cggccggcgg cttcgtcacg tcacccaatg tgccgccgct gccgatcgtc gccggcgtcg
     2761 ctctgccgct cgccgcgttc gtcaccgcgt attcccgttg gggcgggttc cggtccctcg
     2821 tgctgtccgc cgacctgcgc ctgctcaccg gcatccaggc gtggcgctgg gccgggctcg
     2881 gcttcctctt cctctacgcg tacggcgtcc tgcccggtct gttcgccttg cccgcgggcc
     2941 tcggcgacat ggccatcggc ctgacggcgc cgtggatggt cgccaggctg gccagcgacc
     3001 cggcattcgc cgcctcgcgc cgctttgccg tctggaacgc gctcggcatc cttgacctgg
     3061 ccgtcgccgt cggtctcggc ggggccgcct ccgggatcat tcccggcctc acgtcggtcc
     3121 tcaccacgcc gatggcgcgg ctcccgctga tcctgattcc ggccttcctc gtgccgttct
     3181 tcgtcatgct gcatctcacc gccttcttcc aggcgcggcg cgcgtcggcg gtcggctacg
     3241 gcgcggtgcc cgcctgagcc gaacggccgt cagctgcgcc gcgccgcgac cag
//
LOCUS       DASRDG010000226         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_839087, whole genome shotgun sequence.
ACCESSION   DASRDG010000226 DASRDG010000000
VERSION     DASRDG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839087"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFA35_03735"
     CDS             <1..322
                     /locus_tag="VFA35_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT55313.1"
                     /translation="VDGGLLLVIAAENLSGGMGTAAFLAFLMSLCNQRFTATQFALLS
                     AFASVGRVWVGPLAGVLAESIGWPVFFIVSTLVALPALVMLWWMRATVRALEYDPATA
                     PVDD"
     gene            370..1257
                     /locus_tag="VFA35_03740"
     CDS             370..1257
                     /locus_tag="VFA35_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZT55314.1"
                     /translation="MNHDTPARQHVGCRCAFHSRRRFAGGLLLGGAALLTLPAAAREG
                     VEVGSRSSFSQLVSADQIEKAAQQNYLQLMQEASAKGAVAGPSNPQLVRLRAIAQRLI
                     PYTYEWNERARQWRWEVNLINSPQINAFCMPGGKIVFFTGILDKLKLTDDEVAMIMGH
                     EMTHALREHARERMGKTAATQGAIEIGAAIFGLGSGGRYLAGMGGQLLTLKFGRDDES
                     EADLVGMELAARAGYDPHAGVTLWQKMAAASKGAPPQWISTHPSGPTRIRDIERSLPK
                     VEPLYARADKPAQRFDGGR"
     gene            1424..3223
                     /locus_tag="VFA35_03745"
     CDS             1424..3223
                     /locus_tag="VFA35_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZT55315.1"
                     /translation="MTISPDPTPEPIDWPELSRQRRAIVVVDVVESVRLMQANEADVI
                     DRWRRFVNEVRTQVLPAHGGRLVKSLGDGLLLEFGTVPAAVAASLDVQRRIMPFNVGR
                     DANAVMGLRIGVHVAEVVVDALDIYGAGVNLAARLADLAAPGEIAASAEARDALAEGV
                     DADLHDLGECFLRRIDEPVRAYRIETPDSDRSFLALSDKPPDLTPTVAFIPPLPLDSS
                     NANPGLGELIADGVIGRLSRNALLKVISRLSSSALKGRGLAAGEIGKLLDAAYVLSGS
                     YTVGNQRIVVDLELCDTRSGVAVWGDRLAAPLADLIETDSVLIGAAADAVSHHVVEHA
                     LKGAMQRSLPTLSSYAVYLGALSLMHRASRTEFERAKELLDHLIDRHRRVPTPRAWLA
                     QWYVLRLTRGWSREAASDVGLAMNQAQRAIDLDSSCGLAYAMAGFVQCHLKQDLEAAD
                     ELYGQALDANPNEAIAWLFKGVLHTFKGEGPAALEASDRALSLSPLDPLRYYYYSLAA
                     SAAVAAGNYGRAIDLATRSLRSNRTHTSTYRALAMAQALSGREQEARATATQLLVLEP
                     DFTVSGFLRRSPSGRTAAGALYADALRVAGVPE"
BASE COUNT          507 a         1113 c         1194 g          542 t
ORIGIN      
        1 ggtcgacggc gggctgctgc tggtgatcgc ggccgagaac ctctcgggcg gcatgggcac
       61 cgcggccttc ctggcgttcc tgatgagctt gtgcaaccag cgcttcacgg cgacccagtt
      121 tgcgctgctc agcgcgttcg cgtcggtcgg gcgggtctgg gtcggcccgc tcgcgggcgt
      181 gctcgccgag tcgatcggct ggccggtgtt cttcatcgtc tcgacgctgg tcgcgctgcc
      241 cgcgctggtg atgctgtggt ggatgcgcgc cacggtgcgg gcgctcgaat acgatccggc
      301 cacggcgccc gtcgacgatt gaagcggcgc ggcccgcccc gagatacagg ccggactttc
      361 gaggacgcga tgaaccacga cacccctgcg cggcagcacg tcggctgccg ctgcgccttc
      421 cactcgcgcc ggcgcttcgc cggcgggctg ctgctcggcg gcgccgcgct gctgacgctg
      481 ccggcggcgg cgcgcgaggg cgtcgaggtc ggctcgcggt cgagcttcag ccagctcgtc
      541 tcggccgacc agatcgagaa ggcggcgcag cagaactacc ttcagctgat gcaggaggcc
      601 agcgccaaag gcgcggtcgc cggcccgtcg aacccgcagc tcgtgcggct gcgcgcgatc
      661 gcgcagcggc tgatcccgta cacctacgag tggaacgagc gggcgcgcca gtggcgctgg
      721 gaagtgaacc tgatcaacag cccgcagatc aacgccttct gcatgccggg cggcaagatc
      781 gtgttcttca ccggcatcct cgacaagctc aagctcaccg acgacgaggt cgcgatgatc
      841 atgggccacg agatgaccca cgcgctgcgc gagcacgccc gcgagcgcat gggcaagacc
      901 gcggccaccc agggcgcgat cgagatcggc gcggcgatct tcggcctcgg cagcggcggg
      961 cgctacctgg ccggcatggg cggccagctg ctgacgctca agttcggccg cgacgacgaa
     1021 tccgaggccg acctggtcgg catggagctc gcggcgcgcg ccggctacga cccgcacgcc
     1081 ggcgtcacgc tgtggcagaa gatggcggcg gccagcaagg gcgcgccacc gcagtggatc
     1141 tcgacccacc cctccgggcc gacgcgcatc cgcgacatcg agcgcagcct gccgaaggtc
     1201 gagccgctgt acgcgcgcgc cgacaagccg gcgcagcgct tcgacggggg gcggtgacgg
     1261 cggcgggctc ggcggtaggc caggccccgc ggtaggcagg cccgcgccat tgatgcgcgg
     1321 cagaggcgca tcgccgagcg cgcgccatcc ctaatccgga tgaaacatct ggatacttcg
     1381 tggtcgctcc gtcagaatgg ggatgacacc cgaccccacc ctcgtgacga tctcccccga
     1441 ccccacgcca gagccgatcg actggccgga gctgagccgg caacgccgag ccatcgtggt
     1501 ggtggatgtc gtcgagtcgg tgcgactgat gcaggccaac gaggccgacg tcatcgaccg
     1561 ctggcggcgc ttcgtcaacg aggtccgaac ccaggtgctg ccggcgcacg gcggtcggct
     1621 cgtcaagagc ctgggggatg ggctgctgct ggagttcggg actgtgcctg ctgcggtagc
     1681 tgcttcgctg gacgttcagc gcaggatcat gccgttcaac gtcggccgtg atgcgaacgc
     1741 cgtgatgggc cttcgtatcg gggttcatgt ggcagaagtg gtcgttgacg cgctggacat
     1801 ctatggcgcc ggggtcaacc tggctgcgcg gttggccgat cttgcggcgc ccggtgagat
     1861 cgcggcttcg gcggaggccc gcgatgcctt ggccgaagga gtggacgcgg acctgcacga
     1921 tctcggcgaa tgcttcttgc gccgcatcga cgagccggtg cgcgcctacc gaatcgagac
     1981 gcccgattcg gaccgcagtt ttctggcgct gagcgacaag cctcctgacc tgaccccgac
     2041 ggttgcgttc atcccaccgt tgccgcttga ttctagcaac gccaaccccg ggctcggcga
     2101 gctgatcgcg gacggcgtga tcgggcggct gtctcggaac gcgctgctga aggtcatttc
     2161 gcgcctctcg tcttcggcac tgaaaggccg cgggcttgcg gcgggtgaga tcggcaaatt
     2221 gctggatgcg gcctacgtgc tcagcggcag ctataccgtg ggcaaccagc ggatcgttgt
     2281 cgacctggag ctgtgtgata ccaggtcggg cgtggccgtc tggggcgacc gcctcgcggc
     2341 gccgctcgcc gatctcatcg aaaccgacag cgtcctcatc ggggccgccg ccgacgcggt
     2401 gagccaccat gtcgtggagc acgccctgaa aggcgcgatg cagcgttcgc tgccgacctt
     2461 gtcgagttac gcggtctacc tcggcgcgct gtcgctgatg caccgcgcga gccgaactga
     2521 attcgagcgc gccaaggagt tgctcgatca cttgatcgat cggcatcgcc gcgtgccgac
     2581 gccgcgcgcg tggctcgcgc aatggtacgt gctgcggctg actcggggct ggtcacgcga
     2641 agcggcgagc gatgtcggcc tggccatgaa ccaggcgcaa cgcgccatcg acctcgattc
     2701 gtcatgcggc ctagcctatg ccatggcagg cttcgtccaa tgccatctga agcaggatct
     2761 cgaggcggcc gacgagctct acggccaagc actcgatgcg aatccgaacg aggcgatcgc
     2821 ctggctgttc aaaggggtgc tgcacacctt caagggcgag ggccccgctg cgttggaggc
     2881 cagcgacagg gcgctgtcgc tctcgccgct cgatccactg cgctactact actactcgct
     2941 cgccgcatcg gctgccgtgg cggccgggaa ctacggacga gccatcgatc tggcgacacg
     3001 gtccttgcgc tcgaaccgga cgcatacatc gacctatcgg gcgctcgcga tggcgcaggc
     3061 gctcagtgga cgagagcaag aggccagggc gactgcgact cagctgttgg tgttggaacc
     3121 tgacttcacg gtctcggggt tcctgcgcag atcgcccagc ggccgcaccg cggcgggcgc
     3181 tctctatgct gatgcactcc gggtggccgg cgtcccggaa tagcggctct cgccgccgat
     3241 cgattcatgt caaagggaga gaacgtatga gcgcattggg cggcctgggt ggtctggggg
     3301 gccttggcgg tctcggaggg ctcggagggc tcggcgggtt gggtggcctc ggcggc
//
LOCUS       DASRDG010000227         3743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_36287, whole genome shotgun sequence.
ACCESSION   DASRDG010000227 DASRDG010000000
VERSION     DASRDG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36287"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFA35_03750"
     CDS             <1..597
                     /locus_tag="VFA35_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55316.1"
                     /translation="GLTLRAHDHRERHAPWTGSLFALGHGVAIGAIAIGISLLAASFT
                     LPPTLVQIVDWLPIVLLTLLGLWNLRALLAPGAYRPSSVRMKLIPAGLRDRTDLWSTV
                     AIGLLFALVIDTVAHVSAWSVFATHQGGWWAGAVAGLLFSGGMLIASTADSQLVSRVL
                     RSAGSEAAASRLRRGIGWFVVILSFGVVIQTVAAKIAI"
     gene            complement(602..1525)
                     /locus_tag="VFA35_03755"
     CDS             complement(602..1525)
                     /locus_tag="VFA35_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZT55317.1"
                     /translation="MDHRLDTRRVRYFLQVLDCGSIRAAAEALDMDASAVSRAIGTLE
                     RDCGARLLERRGRGVVPTDAGELLAGYMRREHDQKRELLARIDSIQKAERGHIDIVAG
                     EGVVDWLMRASLRIFMAAHPGITVDLDVASTDEIVQRIVDERAHIGIVFRPPKDERLR
                     VHHSHAAPIEALVLETHPLARLGRPLRLADLAPYPGAALHRSFGVRQHVEAAQLSEGV
                     RLQSVLTTSSFAAVGQFVVAGLGYALTVRTSMPSRVDRARLVALSMANPLLSQGQVQV
                     VSRHGRLLSPAAAELLRQIVTDIRQLAPPEV"
     gene            1699..3042
                     /locus_tag="VFA35_03760"
     CDS             1699..3042
                     /locus_tag="VFA35_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HZT55318.1"
                     /translation="MNPTLSSTAAPPHVAILAGGKLRLVVVALVVVLLAELIGPAKFK
                     VGPGLVVLLPMLWALLIAAAWGIAQRRVPAVARIDTGLQGMAGGLLNAALLLFIAKLG
                     LTVGGALPMVKQAGWALFFQEFGHALGTLALGLPLALILGIKREAVGSTFSVGREGNI
                     AIIGEAYGMASPEGRGVLAEYITGTVLGALFIAILAGFITSLHIFDPRSLAMGAGVGS
                     GSLMAAALGAITAQQPAEAVPQLTAIAAASNLLTSVVGFYFTLFLSLPICSWLYHRLE
                     PVLGRMTRRGREPVAEDTPDMSHATAAHTMSFADTVIAWVLMTGGVMIGNLLSFKVPL
                     LDSFVGMLVVVAIVALVEGLKRAFPRVPLILVLSVVATGIGIPGVLPFTGTVLALTGK
                     LNFLAFTTPVLALAGFSVAKDLPLFRRLGWRIVVVSLTATAGTFLGATLIAEFFH"
     gene            3069..>3743
                     /locus_tag="VFA35_03765"
     CDS             3069..>3743
                     /locus_tag="VFA35_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZT55319.1"
                     /translation="MYPDTEIADDIRQSMVAWRRDIHANPETAFEEHRTANVVAHALK
                     LMGLSVHRGLAVTGVVATLKNGDGPSIALRADLDALNMQELGTQAHASKCVGKMHGCG
                     HDGHTAMLLGAAAHLARHKPFRGTVHFVFQPGEENEGGGRVMVEEGLFDQFPADAVYG
                     MHNFPGLPRGQFAIRTGTMTAYLDTFEIVITGKGSHGAMPETGIDSVVVASQLVNALQ
                     TIVSRRT"
BASE COUNT          497 a         1373 c         1280 g          593 t
ORIGIN      
        1 gggctcacgc tgcgcgccca cgaccaccgc gagcgccacg cgccgtggac cggtagcctc
       61 ttcgcgctcg gccacggggt ggcgatcggg gcgatcgcga tcggcatcag cctgctggcc
      121 gcgtcgttca ccttgccgcc gacgctggtg cagatcgtcg actggctgcc gatcgtgctg
      181 ctgacgctgc tcggcctgtg gaacctgcgc gcgctgctcg cgcccggcgc gtaccggccg
      241 agttcggtgc ggatgaagct gatcccggcg ggcttacgcg atcgcaccga cctgtggtcg
      301 acggtcgcga tcgggctgct gttcgcgctg gtgatcgaca ccgtcgcgca cgtctcggcc
      361 tggagcgtgt tcgccaccca ccagggcggc tggtgggccg gcgcggtcgc ggggctgctg
      421 ttctcgggcg gcatgctgat cgccagcacc gccgactcgc agctcgtctc gcgcgtgctg
      481 cgcagcgcgg gcagcgaggc cgccgcgtcg cgcctgcgcc gcggcatcgg ctggttcgtc
      541 gtgatcctgt cgttcggcgt cgtgatccag acggtggccg cgaagatcgc catctgatac
      601 gtcagacctc cggcggcgcc agctgccgga tgtccgtcac gatctggcgc agcagttccg
      661 ccgcggccgg cgacagcagc cggccgtgac gcgacaccac ctgaacctgc ccctgcgaca
      721 gcaacgggtt cgccatcgac agggcgacga ggcgtgcgcg atcgacccgc gacggcatcg
      781 aggtccgcac cgtcagcgca taccccaagc cggcgacgac gaactgtccg accgcggcga
      841 acgagctcgt cgtcagcacg gattgcaggc gcacgccctc gctgagttga gccgcctcga
      901 catgctggcg cacgccgaag ctgcggtgca gcgcggcgcc ggggtacggc gccaggtcgg
      961 cgagccgcag cggccgcccc agccgcgcga gcgggtgcgt ctcgagcacc agggcctcga
     1021 tcggcgccgc gtgcgaatgg tgcacgcgca gccgttcgtc cttcggcggc cggaacacga
     1081 tcccgatgtg cgcgcgttcg tcgacgatgc gctgcacgat ctcgtcggtg ctcgcgacgt
     1141 cgagatcgac cgtgatgcca gggtgcgcgg ccatgaagat gcgcaggctc gcccgcatca
     1201 gccagtcgac gacgccctcg ccggcgacga tgtcgatgtg gccgcgctcg gccttctgga
     1261 tgctgtcgat ccgggccagc agttcccgct tctggtcgtg ctcgcggcgc atgtagcccg
     1321 ccagcagttc gccggcatcg gtcggcacga cgccgcggcc gcggcgctcg agcagccgcg
     1381 cgccgcagtc gcgttcgagc gtgccgatcg cccggctcac cgccgaagcg tccatgtcga
     1441 gcgcctccgc cgcagcgcgg atcgagccgc agtccagcac ctgcaggaag tagcgaaccc
     1501 ggcgtgtgtc gaggcgatgg tccatcgcga tgctgtccgt caccggcgtg cgttgcattt
     1561 catgcaacga atatcccatc ggcgtgtcat tgatgcaaga gggcgctccc cggacacttt
     1621 cattgccgcg ggcctacgcg gcatcgggcg ccggatcccc tggcgcagcc tcttccctct
     1681 tgatcgagaa cagcgcccat gaacccaacc ctctcctcga ccgcggcccc gccgcacgtc
     1741 gccatcctcg ctggcggcaa gctcaggctc gtcgtcgtcg ccctcgtcgt cgtgctgctg
     1801 gccgagctga tcggcccggc gaagttcaag gtcggccccg gcctggtggt gctgctgccg
     1861 atgctgtggg cgctgctgat cgccgccgcc tggggcatcg cgcagcgccg cgttccggcg
     1921 gtcgcgcgca tcgacaccgg cctgcaaggc atggccggcg gcctgctcaa cgccgcgctg
     1981 ctgctgttca tcgcgaagct cggcctgacg gtcggcggcg cgctgccgat ggtcaagcag
     2041 gccggctggg cgctgttctt ccaggagttc ggccacgcgc tcggcacgct cgcgctcggg
     2101 ctgccgctcg cgctgatcct gggcatcaag cgcgaggcgg tcggctcgac cttctcggtc
     2161 ggccgcgagg gcaacatcgc gatcatcggc gaggcctacg gcatggcctc gcccgagggc
     2221 cgcggcgtgc tcgccgagta catcaccggc accgtgctgg gcgcgctgtt catcgcgatc
     2281 ctcgccggct tcatcaccag cctgcacatc ttcgacccgc gttcgctggc gatgggcgcc
     2341 ggcgtcggct cgggcagcct gatggccgcg gcgctcggcg cgatcaccgc gcagcagccg
     2401 gccgaggcgg tgccgcagct caccgcgatc gccgcggcgt cgaacctgct gacctcggtg
     2461 gtcggcttct acttcacgct gttcctgtcg ctgccgatct gctcgtggct gtaccaccgg
     2521 ctcgagcccg tgctcggccg catgacccgg cgcggccgcg agccggtcgc cgaagacacg
     2581 cccgacatga gccacgccac cgcggcgcac acgatgtcgt tcgccgacac cgtcatcgcc
     2641 tgggtgctga tgaccggcgg cgtgatgatc ggcaacctgc tgtcgttcaa ggtgccgctg
     2701 ctcgactcgt tcgtcggcat gctcgtcgtc gtcgcgatcg tcgctctcgt cgaggggctg
     2761 aagcgcgcgt tcccgcgcgt gccgctgatc ctggtgctgt cggtggtcgc caccggcatc
     2821 ggcatcccgg gcgtgctgcc gttcaccggc accgtgctcg cgctgaccgg caagctgaac
     2881 ttcctggcgt tcacgacgcc ggtgctcgcg ctggccggct tctcggtggc gaaggacctg
     2941 ccgctgttcc gccggctcgg ctggcgcatc gtggtggtgt cgctgaccgc gaccgccggc
     3001 accttcctcg gcgcgacact gatcgccgag ttcttccact gaccaccgac cgacccgagg
     3061 accgcaccat gtaccccgat accgagattg ccgacgacat ccgccagagc atggtcgcgt
     3121 ggcggcgcga catccacgcg aaccccgaaa ccgccttcga ggagcaccgc accgccaacg
     3181 tcgtcgcgca tgcactgaag ctgatgggcc tgtcggtgca ccgcggcctg gcggtgaccg
     3241 gcgtcgtcgc cacgctgaag aacggcgacg gcccgagcat cgcgctgcgc gccgacctcg
     3301 acgcgctgaa catgcaggag ctgggcacgc aggcgcatgc gtcgaagtgc gtcggcaaga
     3361 tgcacggctg cggccacgac gggcacaccg cgatgctgct cggcgccgcc gcgcacctgg
     3421 cgcgccacaa gccgttccgc ggcaccgtgc acttcgtctt ccagcccggc gaggagaacg
     3481 agggcggcgg ccgcgtcatg gtcgaggaag gcctgttcga ccagttcccg gccgatgcgg
     3541 tgtacggcat gcacaacttt ccgggcctgc cgcgcgggca gttcgcgatc cgcaccggca
     3601 ccatgacggc ctacctcgac accttcgaga tcgtcatcac cggcaagggc agccacggtg
     3661 ccatgcccga gaccggcatc gactcggtgg tggtggcatc gcagctcgtc aacgcgctgc
     3721 agaccatcgt cagccgccgc acc
//
LOCUS       DASRDG010000228         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_490211, whole genome shotgun sequence.
ACCESSION   DASRDG010000228 DASRDG010000000
VERSION     DASRDG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490211"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFA35_03770"
     CDS             <1..809
                     /locus_tag="VFA35_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZT55320.1"
                     /translation="TVGKGIVLPGVVDHHVVFGALIGAIFWNVITWWWGIPSSSSHAL
                     IGGIVGAVIAKAGASQLIAAGILKTVLFIFLSPLLGFLLGSLLMVLVANLCRHTSPLR
                     VDNLFRRLQLVSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASGADTMPPGWVIWACY
                     LAIGAGTMFGGWRIVKTMGQKITKLKPVGGFCAEAGGAITLFLATALGVPVSTTHTIT
                     GAIVGVGSVQRASAVRWGVAGNIVWAWIFTIPASAFVAGVFYWIGLAVLP"
     gene            complement(779..1249)
                     /locus_tag="VFA35_03775"
     CDS             complement(779..1249)
                     /locus_tag="VFA35_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HZT55321.1"
                     /translation="MTADSPGHKSKTLATWLALLGGSVGLHRFYLHGFRDAWGWLFVA
                     PSLVGLYGVQRMRHLGVDDHVAWVLIPFLGAVLSAAMLSAIVYGLTPDEKWNARFNPA
                     GPDHRMNWATVIGVATALIVGGGVLMATLAFSAQRYFEYQAETAAQGKTASPIQ"
     gene            complement(1246..1788)
                     /locus_tag="VFA35_03780"
     CDS             complement(1246..1788)
                     /locus_tag="VFA35_03780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT55322.1"
                     /translation="MSSPALLIRPSTPADLPAITAIYGWNVLHGTGTFELDAPDQAEM
                     ARRRDDVRGKGLPWLVVERGGAVQGYAYANHFRPRRAYRFCLEDSLYLAADAKGQGLG
                     RLLLAELMAQCEALGARQMLAVIGDSHNLGSVGVHRALGFEHIGVMKSAGWKFDRWLD
                     VVIMQKALGPGDTTGGKDLA"
     gene            1968..2216
                     /gene="rpsP"
                     /locus_tag="VFA35_03785"
     CDS             1968..2216
                     /gene="rpsP"
                     /locus_tag="VFA35_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZT55323.1"
                     /translation="MVVIRLSRGGAKKRPFFNIVVADSRTRRDGRFIERVGFYNPVAS
                     GGEQPLRVAFDRIEHWTSTGAQMSPTVQRLVAQAKTAA"
     gene            2251..>2459
                     /locus_tag="VFA35_03790"
     CDS             2251..>2459
                     /locus_tag="VFA35_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55324.1"
                     /translation="MNAAARPPGADPAWPVDAIEVGRIGEAWGLKGWIKVHPFAANPQ
                     ALFSSRRWFLLPPEKAGPLRPAAGSA"
BASE COUNT          348 a          822 c          861 g          428 t
ORIGIN      
        1 cgactgtcgg caagggcatc gtgctgccgg gcgtcgtgga ccatcacgtc gtgttcgggg
       61 ccctcatcgg cgcgatcttc tggaacgtga tcacctggtg gtggggcatt ccgtcgagtt
      121 cttcgcacgc gctgatcggc ggcatcgtcg gcgcggtgat cgcgaaggcc ggcgccagcc
      181 aactgatcgc ggccggcatc ttgaagacgg tgctgttcat ctttctctcg ccgctgctcg
      241 gctttctgct cggctcgctg ctgatggtgc tggtcgcgaa cctgtgtcgg cacacctcgc
      301 cgctgcgggt cgacaacctg ttccggcgcc tgcaactggt ctcggccggg ctctacagcc
      361 tcggccacgg cggcaacgac gcgcagaaga ccatcggcat catctggatg ctgttgattg
      421 ccagcggtta cgcgagcggc gccgacacga tgccgccggg ctgggtgatc tgggcctgct
      481 acctcgcaat cggcgccggc acgatgttcg gcggctggcg catcgtcaag accatggggc
      541 agaagatcac gaagctcaag ccggtgggtg gcttctgcgc cgaggcgggc ggggcgatca
      601 cgctgtttct cgcgaccgcg ctgggcgtgc cggtgtcgac gacgcacacg atcaccgggg
      661 caatcgtcgg cgtcggctcg gtgcagcgcg cgtcggccgt gcgctggggc gtggccggca
      721 acatcgtctg ggcgtggatc ttcacgatcc cggcctcggc cttcgtcgcc ggcgtcttct
      781 actggatcgg gctcgcggtc ttgccttgag cagccgtttc ggcctggtac tcgaagtagc
      841 gctgggcgct gaaagcgagg gttgccatca gcacgccgcc gccgacgatc aaggcggtcg
      901 ccacaccgat caccgtggcc cagttcatcc ggtgatcggg gccggcgggg ttgaaccgcg
      961 cgttccactt ctcgtccggc gtcaggccgt agacgatcgc gctgagcatc gcggccgaca
     1021 gcactgcgcc gaggaacggg atcagcaccc aggcgacgtg atcgtccacc ccgaggtggc
     1081 gcatgcgctg cacgccgtac aggcccacca ggctgggcgc gacgaacagc cagccccatg
     1141 cgtcgcgaaa gccgtgcagg tagaagcggt gcaggccgac gctgccgccg agcagcgcga
     1201 gccaggtcgc cagcgtcttc gacttgtggc ccggcgaatc cgcggtcatg cgagatcctt
     1261 gccgccggtc gtgtcacccg ggccgagcgc cttttgcatg atcacgacgt cgagccagcg
     1321 gtcgaacttc cagcccgccg acttcatcac gccgatgtgc tcgaaaccca gggcgcggtg
     1381 cacgccgacc gagccgaggt tgtgcgaatc gccgatcacc gcgagcatct ggcgtgcgcc
     1441 cagcgcctcg cactgcgcca tcagttcggc cagcaacagc cggcccaatc cctggccctt
     1501 ggcatcggcg gcgaggtaga gcgagtcttc gaggcagaag cggtaggccc tgcgcgggcg
     1561 gaagtgattg gcgtaggcgt agccctgcac cgcgccgccg cgctcgacca cgagccaggg
     1621 caggcccttg ccgcgcacgt cgtcgcggcg gcgcgccatc tcggcttggt cgggtgcgtc
     1681 gagctcgaag gtgccggtgc cgtgcagcac gttccagccg tagatcgcgg tgatggcggg
     1741 caggtcggcg ggggtgctgg ggcggatcag caaagcgggg ctgctcatgg cgagtcctgt
     1801 aggtcggtgc ttgagtttat aatgcgcggt ttcgttggtg gtcggccgag cggccggcgt
     1861 atctacaccg tcgattggcg agaaccttct gcctgccctg cgcgctttct ctcggaagcg
     1921 cggtgtcctc ggcgcggtgg acgctttgaa catattcagg aacagtcatg gtcgtgatcc
     1981 gtctttctcg tggtggtgcc aagaagcgcc cgttcttcaa catcgtcgtc gccgactcgc
     2041 gcacccgacg cgatggccgc ttcatcgagc gcgtgggttt ctacaacccg gtcgcctcgg
     2101 gtggcgagca gccgctgcgc gtcgcgttcg accgcatcga gcactggacc agcaccggcg
     2161 cgcagatgtc gccgacggtg cagcgcctcg tcgcgcaagc caagaccgcg gcctgagccg
     2221 cggtcgggcg cctggatcgg gtcgacggcg gtgaacgctg ctgcccgccc gcccggcgca
     2281 gacccggcct ggcccgttga tgcgatcgaa gtcggccgca tcggtgaggc ctggggcctg
     2341 aagggctgga tcaaggttca tccctttgcc gccaacccac aggcgctgtt ctcctcgcgc
     2401 cgctggttcc tgctgccgcc cgagaaggcc ggcccgctgc ggccggccgc aggcagcgc
//
LOCUS       DASRDG010000229         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_36549, whole genome shotgun sequence.
ACCESSION   DASRDG010000229 DASRDG010000000
VERSION     DASRDG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36549"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFA35_03795"
     CDS             <1..848
                     /locus_tag="VFA35_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZT55325.1"
                     /translation="GNGGEPPARGASAAVGPRGIEPAQVSATAPSAPAGEIARQDLLP
                     AGPRAVLALGRTAVGLGAAPPDELLADLGCTATAMDDRDAIELVLAGRAAFAVVGQMP
                     SAQDVRAGLRGTLLGVELWSLAVPAGSPLASLRCEQVRAVLAGQARSWQDLGFLGGAI
                     TLVVPDDRQTAERAARALLPGDPLAVAAMRVAGDDAVLDQVARTDGALGVVRATAAAR
                     SPAVRLLRLEGVLPSFAAFAQGQYPAGVPLVVVAPAGALAAAAQRYLGAVRAAPGAGW
                     LTLPP"
     gene            complement(877..1644)
                     /locus_tag="VFA35_03800"
     CDS             complement(877..1644)
                     /locus_tag="VFA35_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55326.1"
                     /translation="MSSRRCALLLALSALAGCVPGHYRVDAGAAFTALDGRIGLQNSS
                     GTLVLADNMNDFAGNLGLGDTESSPYVRAEADFGDHRVKVSYLANHGTGTGTLGGAFG
                     DIPAGTAVTTDLDFTNVTASWSYDLAPTSLLRVAPGVQVGYYNFDISVRSTTPVAFET
                     VNTDLIAPMPYLDVEVDLGIVDAGVNIGLMSANLHDATSRYTDAEAFARVTPFPGLEL
                     IAGYRYILMDAHGSASGRDFDADFVVEGFFLGGGIHF"
     gene            complement(1723..>2520)
                     /locus_tag="VFA35_03805"
     CDS             complement(1723..>2520)
                     /locus_tag="VFA35_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55327.1"
                     /translation="HADARMNRTAPAHFAAAFAAAFAAALGCGSCTQMHGTAQAGYSQ
                     MAIDGRVALSSTGGGASIDQDVDSAFGIGDPQGAPYVRAQFDFGTPVITASAFTFDER
                     GSGTLNANFGSITSGTPVDTDLRFTDAKVSLAFDIPLGPIKIAPGLAADLIYLEMNVR
                     STLSASSEELKVFAPVPMGFVRAEADLGLVGLVGEFGYVKNPNIGDVEGTLWDGEVMA
                     ELRAGRALLLFAGYRTISVNGSGTVDNQDFSTDLRISGWTIGGGLRF"
BASE COUNT          319 a          869 c          980 g          352 t
ORIGIN      
        1 tcggcaacgg tggcgaaccg ccggcgcgcg gggcgtccgc cgcggtcggc ccgcgcggga
       61 tcgagcccgc gcaggtgtcg gcgacagcgc cgtcggcgcc ggccggcgag atcgcgcgcc
      121 aggacctgct gcccgccggg ccgcgcgccg tactcgcgct gggccgcacg gccgtcggcc
      181 tcggcgccgc gccgccggac gaactgctcg cggacctcgg ctgcaccgcg accgcgatgg
      241 acgatcgcga cgcgatcgag ctggtgctcg cggggcgcgc cgcgttcgcg gtggtcggcc
      301 agatgccgtc cgcgcaggac gtgcgcgccg gtctgcgcgg cacgctgctc ggcgtcgagt
      361 tgtggtcgct cgcggtgccg gccggcagtc cgctggcctc gttgcgctgc gaacaggtgc
      421 gcgcggtgct cgcgggccag gcgcgctcgt ggcaggacct cggtttcctc ggcggcgcga
      481 tcacgctggt cgtgcccgac gaccggcaga cggccgaacg cgcggcgcgc gcgctgctcc
      541 ccggcgatcc gctggcggtg gcggcgatgc gcgtcgccgg cgacgacgcg gtgctggacc
      601 aggtggcgcg caccgacggc gcgctcggcg tcgtgcgcgc gaccgccgcc gcgcgatcgc
      661 ccgcggtgcg gctgctgcgg ctcgagggtg tcctgccgtc gttcgcggcg ttcgcgcagg
      721 gtcagtaccc ggccggcgtg ccgctggtcg tggtcgcgcc ggcgggcgcg ctcgcggccg
      781 cggcgcagcg ctacctcggt gcggtccgcg ccgcgccggg cgccggctgg ctcacgctgc
      841 cgccctgacc gcagcgctgc cagcgcgcgg cgcgcgtcag aagtggatgc cgccgccgag
      901 gaagaaccct tcgaccacga agtcggcgtc gaagtcgcgg cccgaggcgc tgccgtgcgc
      961 gtccatcagg atgtagcggt agccggcgat cagttcgagg ccggggaacg gggtcacgcg
     1021 tgcgaacgcc tcggcgtccg tgtagcgcga cgtcgcatcg tgcaggttgg ccgacatcag
     1081 gccgatgttc acgccggcgt cgacgatgcc gaggtcgacc tcgacgtcga ggtacggcat
     1141 cggcgcgatg aggtcggtgt tgacggtctc gaacgcgacc ggcgtcgtcg agcgcaccga
     1201 gatgtcgaag ttgtagtagc cgacctgcac gccgggggcg acgcgcagca ggctcgtcgg
     1261 cgccaggtcg tagctccacg acgcggtgac gttggtgaag tcgaggtcgg tggtgacggc
     1321 ggtgccggcc ggaatgtcgc cgaacgcgcc gccgagcgtg ccggtgccgg tcccgtggtt
     1381 cgccaggtac gagaccttga cgcggtggtc gccgaagtcg gcctccgcgc gcacgtacgg
     1441 cgacgactcg gtgtcgccga ggccgaggtt gccggcgaag tcgttcatgt tgtccgccag
     1501 cacgagcgtg ccgctgctgt tctgcaggcc gatgcggccg tcgagcgcgg tgaaggcggc
     1561 gccggcgtcg acgcggtagt ggcccggcac gcagcccgcg agggcggaga gggcgagcag
     1621 caacgcacag cgtctcgaac tcatgttctg tccttcctgg agttgtccgc tcggagactg
     1681 agaagagaat cccgcgccga ggccgccttc ggcgtccccg actcagaagc gcaggccgcc
     1741 gccgatggtc cagcccgaga tgcgcaggtc ggtcgagaag tcctggttgt cgaccgtgcc
     1801 gctgccgttg accgagatcg tgcggtagcc ggcgaacagg agcagcgcgc ggccggcgcg
     1861 cagctcggcc atcacctcgc cgtcccagag cgtgccctcc acgtcgccga tgttggggtt
     1921 cttgacgtag ccgaactcgc cgaccagccc gacgaggccg aggtccgcct cggcgcggac
     1981 gaagcccatc ggcaccggcg cgaacacctt gagttcctcg ctcgacgcac tcagggtgct
     2041 gcgcacgttc atctccaggt agatgaggtc ggcggcgagg cccggcgcga tcttgatcgg
     2101 gccgagcggg atgtcgaacg cgagcgacac cttcgcgtcg gtgaagcgga ggtcggtgtc
     2161 gaccggcgtg ccgctcgtga tgctgccgaa gttcgcgttg agcgtgcccg agccgcgctc
     2221 gtcgaacgtg aacgcggacg ccgtgatcac gggggtgccg aaatcgaact gcgcgcgcac
     2281 gtacggcgcg ccctgcgggt cgccgatgcc gaatgcgctg tcgacgtcct ggtcgatcga
     2341 ggcgccgccg ccggtgctcg acagcgcgac gcggccgtcg atcgccatct gcgagtaacc
     2401 ggcctgggca gtcccgtgca tctgcgtgca cgaaccgcag ccgagcgcgg cggcgaacgc
     2461 agcggcgaac gcagcggcga aatgggcggg agcggtgcgg ttcatgcggg cgtcggcgtg
//
LOCUS       DASRDG010000230         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_395795, whole genome shotgun sequence.
ACCESSION   DASRDG010000230 DASRDG010000000
VERSION     DASRDG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395795"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..605
                     /gene="soxY"
                     /locus_tag="VFA35_03810"
     CDS             147..605
                     /gene="soxY"
                     /locus_tag="VFA35_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HZT55328.1"
                     /translation="MQNRREMLTRSAAVAALLAGAGVLPARAQAAYNKAAFEAKSIDD
                     VVKALGIARPVESKDIVFTAPDIAENGAVVPIAASTALPGVKRIAFLVEKNPTTLAGV
                     FDFSDAVETSVKTRIKMGQSSNVLAVAILGDGKVLYAQRDVKVTLGGCGG"
     gene            623..934
                     /gene="soxZ"
                     /locus_tag="VFA35_03815"
     CDS             623..934
                     /gene="soxZ"
                     /locus_tag="VFA35_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HZT55329.1"
                     /translation="MSDPMRIRAQAQGGGAVVRVLMSHEMETGQRKDAAGKTIPAWHI
                     TEVSAALNGKTVLTAQWGTAIAKNPFTEFVVKGAKAGDKITVSWVDNRGDKRSDEAVI
                     A"
     gene            931..1731
                     /gene="soxA"
                     /locus_tag="VFA35_03820"
     CDS             931..1731
                     /gene="soxA"
                     /locus_tag="VFA35_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HZT55330.1"
                     /translation="MTQARSLIAAACLAAAGAVQAQTSTADGIAQYRALLADGNPAEL
                     WEIKGEGLWKQRRGPRNASLEACDLGKGPGVVPGAFVELPRFFADTRRVQDLESRLLS
                     CMQALQGFDAAAIAATPFGRGEQANLEALVAYIAAASRGLRFNLPQGHAEERKMAEVG
                     KRVFFFRAGPYDFACASCHDAAGKRIRLQELPNLTLNPGDGVGFAAWPAYRVSSGELW
                     TMQRRLNDCYRQQRFPYPGYASDASIALGVYMGVQARGAVSSAPSIKR"
     gene            1739..2380
                     /gene="soxX"
                     /locus_tag="VFA35_03825"
     CDS             1739..2380
                     /gene="soxX"
                     /locus_tag="VFA35_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HZT55331.1"
                     /translation="MSILPRIRIRIGALALLAGCAGAPGPQDLDRATQAVMQNSFRAQ
                     GIATLDRLVQDEVQQACSAETPPPPARAAQLEAAQLASVQWPQDGVYLGDWREGEKLA
                     QNGRGGTWSDAPGVPAGGNCYNCHQLAKAEIAFGTIGPSLYQYGKLRGVADPADPAAA
                     AVVRYTWGKLWNAKAYNACSAMPRFGHQGVLTPAQIKDLMALLLDPKSPVNAP"
     gene            2418..3401
                     /locus_tag="VFA35_03830"
     CDS             2418..3401
                     /locus_tag="VFA35_03830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZT55332.1"
                     /translation="MTTPPQRSSRPAISCVVPAYNEAANLGLLLSGLTAQLAELTDRW
                     EVIVIDDGSRDATPAAMAPWVVEPGVRYVRLSRNFGKEAALTAGIDRAVGDVVLLMDA
                     DLQHPASLVPEMVAAWRDGADMVCAARASRADESWPKRLGTAIFYRLVNHNSPVPIPV
                     DAGDFRLMDRRVVDALKALPERNRFMKGLYAWVGFHNVIIPYVPAERHAGVSTFSLRS
                     LSKLAFTGVTAFTNAPLRLWSALGVIVALFALAFGAWIVVEHFIYGSDVPGWATLVCG
                     MMFFSGVQLLSIGILGEYVGRIFDEVKQRPVYLIGSEAGRGAIRAPGEPPA"
     gene            3398..>4089
                     /locus_tag="VFA35_03835"
     CDS             3398..>4089
                     /locus_tag="VFA35_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HZT55333.1"
                     /translation="MTPPDRAPGRPKPDRVPPGDRPRYTAGEGHRVLGLCADDFGLAP
                     GISTAIATLAHARRLTAISCITNSPHWEASAALLHDLPDTVDVGLHLNFTEGRPLSAR
                     LARRWPQLPSLPRLLVQAHLRLLPLAQVRNEVHAQLAAFNRALGRPPAFIDGHQHVHH
                     LPGLRRVILDMVEHVQPLPTMRNTGRVLGPGAGFKRRVIEATGGRALAAELVQRVIAH
                     NPALLGAYDFAAP"
BASE COUNT          603 a         1508 c         1430 g          548 t
ORIGIN      
        1 caggcgcaga tcgaggcggc ggtggcccgc gaagccccgg aatatccctg agcccgggcg
       61 gttctgcgac accgcccggg gcaagggcgc gatttcggcc ctacagttcc gggtcgcgcg
      121 aatggccgcg ccaggaactc cgccccatgc aaaaccgacg agagatgctg acccgcagcg
      181 ccgccgtggc cgcgctgctg gccggcgctg gcgtgctgcc ggcccgggcc caggccgcgt
      241 acaacaaggc cgcgttcgaa gccaagtcga tcgacgatgt ggtcaaggcc ctcggcatcg
      301 ccaggccggt cgagagcaag gacatcgtct tcaccgcgcc cgacatcgcc gagaacggcg
      361 ccgtcgtgcc gatcgccgcg agcaccgcgc tgcccggcgt caagcgcatc gccttcctgg
      421 tcgagaagaa cccgaccacg ctggccggcg tgttcgactt cagcgacgcc gtcgagacca
      481 gcgtcaagac gcgcatcaag atgggccaga gctcgaacgt gctcgcggtc gcgatcctcg
      541 gcgacggcaa ggtcttgtac gcgcagaggg acgtcaaggt cacgctcggt ggctgcggcg
      601 ggtagccatt agggagaacg acatgtccga cccgatgcgc attcgcgccc aggcccaagg
      661 cggcggcgcc gtcgtgcgcg tgctgatgag ccacgagatg gagaccggcc agcgcaagga
      721 cgccgccggc aagacgattc ccgcctggca catcaccgag gtcagcgcgg cgctgaacgg
      781 caagaccgtg ctgaccgcgc agtggggcac ggcgatcgcg aagaacccgt tcaccgagtt
      841 cgtcgtgaag ggcgcgaagg ccggcgacaa gatcaccgtc agctgggtcg acaaccgcgg
      901 cgacaagcgc tcggacgagg ccgtgatcgc gtgacgcaag cgcgcagcct catcgccgcc
      961 gcgtgcctcg cggcggccgg cgcggtgcag gcgcagacca gcaccgccga cggcatcgcg
     1021 cagtaccgcg cgctgctcgc cgacggcaac ccggccgagc tctgggagat caagggcgaa
     1081 gggctgtgga agcaacggcg cggcccgagg aacgcctcgc tcgaagcctg cgacctcggc
     1141 aagggcccgg gcgtggtccc cggcgcgttc gtcgaactgc cgcgcttctt tgccgacacg
     1201 cggcgcgtgc aggacctcga gtcgcgcctg ctgagctgca tgcaggcact gcagggcttc
     1261 gacgccgccg cgatcgccgc gacgccgttc ggccggggcg agcaggccaa cctggaggcg
     1321 ctggtcgcct acatcgcggc cgcctcgcgc gggctccggt tcaatctgcc gcagggccac
     1381 gccgaagaac gcaagatggc cgaagtgggc aagcgcgtgt tcttcttccg cgccggcccg
     1441 tacgacttcg cgtgcgcgtc gtgccacgac gcggccggca agcgcatccg cctgcaggag
     1501 ctgccgaacc tgacgctgaa ccccggcgac ggcgtcggct tcgcggcgtg gccggcctac
     1561 cgcgtgtcga gcggcgagct ctggaccatg cagcggcgcc tgaacgactg ctaccgccag
     1621 cagcgcttcc cgtacccggg ctacgcgtcg gacgcttcaa tcgcgctcgg cgtctacatg
     1681 ggcgtgcagg cccgcggcgc ggtctcgtcc gcgccgtcga tcaagcgttg acgtgccgat
     1741 gagcatcttg ccccgcatcc gcatccgcat cggcgcgctc gcgctgctcg ccggctgcgc
     1801 cggcgcgccg ggcccgcaag acctcgaccg cgccacgcag gcggtgatgc agaactcgtt
     1861 ccgcgcgcag ggcatcgcca cgctcgaccg gctcgtgcag gacgaggtgc aacaagcctg
     1921 ctcggccgag acgccgccgc cgcccgcgcg cgcggcccag ctcgaagccg cgcagctcgc
     1981 cagcgtgcag tggccgcagg acggcgtcta cctcggcgac tggcgcgaag gcgagaagct
     2041 cgcgcagaac ggccgcggcg gcacctggag cgacgcgccc ggcgtgcccg cgggcggcaa
     2101 ctgctacaac tgccaccagc ttgccaaggc cgagatcgcg ttcggcacga tcgggccgag
     2161 cctgtaccag tacggcaagc tgcgcggcgt cgccgacccg gccgacccgg ccgcggccgc
     2221 ggtcgtccgc tacacctggg gcaagctctg gaacgcgaag gcctacaacg cctgctcggc
     2281 gatgccgcgc ttcggccacc aaggcgtgct gaccccggcg cagatcaagg acctgatggc
     2341 gctgctgctc gatccgaagt cgccggtgaa cgcgccttga acagccccgc gccgcgccag
     2401 cgtaaagtgg cgccagcatg acgaccccac cccagcgctc ctcccgtccc gcgatcagct
     2461 gcgtcgtacc cgcctacaac gaggctgcca acctcggcct gctgctgagc ggcctgaccg
     2521 cgcagctcgc cgagttgacc gaccgctggg aggtcatcgt gatcgacgac ggcagccgcg
     2581 acgccacgcc cgcggcgatg gcgccgtggg tcgtcgagcc gggcgtgcgc tatgtgcgcc
     2641 tgtcgcgcaa tttcggcaag gaggccgcgc tcaccgccgg catcgaccgc gccgtcggcg
     2701 atgtcgtgct gctgatggac gccgacctgc agcaccccgc ctcgctcgtg cccgagatgg
     2761 tggcggcgtg gcgcgacggc gccgacatgg tctgcgccgc gcgcgcctcg cgcgccgacg
     2821 agagctggcc gaagcgcctg ggcaccgcga tcttctaccg gctcgtgaac cacaactcgc
     2881 cggtgccgat tccggtcgat gcgggcgact tccggctgat ggaccgacgc gtcgttgacg
     2941 cgctgaaggc gctgcccgag cgcaaccgct tcatgaaggg cctgtacgcc tgggtcggct
     3001 tccacaacgt gatcattccg tacgtgccgg ccgagcggca cgcaggcgtc agcaccttct
     3061 cgctgcgcag cctgtcgaag ctcgcgttca ccggcgtcac cgcgttcacg aacgcgccgc
     3121 tgcgcttgtg gagcgcgctc ggcgtgatcg tcgcgctgtt cgcgctcgcg ttcggcgcgt
     3181 ggatcgtggt cgagcacttc atctacggct ccgatgtgcc gggctgggcc acgctggtgt
     3241 gcgggatgat gttcttcagt ggcgtgcagc tgttgtcgat cggcatcctc ggcgagtacg
     3301 tcgggcgcat cttcgacgag gtcaagcagc ggccggtcta cctgatcggc agcgaggccg
     3361 ggcgcggcgc gatccgcgcg ccgggcgaac cgccggcatg acgccaccgg accgagcgcc
     3421 gggccgcccc aagccggacc gggtcccccc gggggaccga ccgcggtaca ccgcgggcga
     3481 ggggcaccgt gtcctcgggc tctgcgccga cgacttcggt ctcgcgccgg gcatctccac
     3541 ggccatcgcg acgctggcgc atgcgcggcg gctcaccgcg atctcgtgca tcacgaacag
     3601 cccgcactgg gaagcgtccg ccgcgctgct gcacgacctg cccgacacgg tcgatgtcgg
     3661 cctgcacctg aacttcaccg agggccggcc gctcagcgcg cggctcgcgc gccgctggcc
     3721 gcagctgcct tcgttgccgc ggctgctggt gcaggcgcac ctgcggctgc tgccgctcgc
     3781 gcaggtgcgc aacgaggtgc atgcgcagct cgcggcgttc aaccgcgcgc tcggccggcc
     3841 gcccgcgttc atcgacggcc accagcacgt gcaccacctg ccgggcctgc gccgggtcat
     3901 tctcgacatg gtcgagcacg tgcagccgct gccgacgatg cgcaacaccg gccgggtgct
     3961 cggccccggc gcgggcttca agcgccgcgt gatcgaagcc accggcgggc gcgcgctcgc
     4021 ggccgaactc gtgcagcgcg tgatcgccca caacccggcg ctgctgggcg cctacgactt
     4081 cgctgcgcc
//
LOCUS       DASRDG010000231         5458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_266539, whole genome shotgun sequence.
ACCESSION   DASRDG010000231 DASRDG010000000
VERSION     DASRDG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266539"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFA35_03840"
     CDS             complement(<1..511)
                     /locus_tag="VFA35_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55334.1"
                     /translation="MPTLLALPNPCRPATGCAPRWVPALLAALCGASLAAAPPIPTAD
                     FVRRGNFFAPVMSPDGKLLVVTQRTPKEPRDEYATVVYQLDGMKLLSAVRSQAGDLPL
                     RHAWVTDTRIVTMVGREFGTVGEPLATGELIAMNSDGRGIQYLYGYRRGMSEATFGKD
                     SFGNLANDSG"
     gene            complement(688..1023)
                     /locus_tag="VFA35_03845"
     CDS             complement(688..1023)
                     /locus_tag="VFA35_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HZT55335.1"
                     /translation="MPDIHIQREHKLGLAKARKVALQWAIDAEEKFGMACTIVEGKTS
                     DTVEFTRSGVTGELLVEAHRFTVDAKLGFLFGAFSKTIVAEIEKNLDALLAKSKPATK
                     KAAAKTSKR"
     gene            complement(1035..2096)
                     /locus_tag="VFA35_03850"
     CDS             complement(1035..2096)
                     /locus_tag="VFA35_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZT55336.1"
                     /translation="MAFLGALGVAVLAAATVCLASGCSTIGYYAQSVGGHLAIVRAAR
                     PVPEWLADPHTSADLRARLELSQRIRDFAVSELKEPDNASYRRYADLRRGAAVWNVVA
                     APELSLRLQTWCFPVVGCVGYRGYYDPAEANAFAASLKPLGLEVSVYAVPAYSTLGLL
                     PGDALADPLLNTFIDDTEGELARLIFHELAHQIAFAKGDTVFNESFATAVERIGSARW
                     LATHASSQARDEYEQVASRRRDFRELTARYRARFDVLYDSPASDADKRAGKAALMREL
                     RAEFDATKRARWGGYSGYDGWLARANNASFGVLAAYNELVPNFERLFEREGRDFTRFY
                     AEVRRLAALPLAERRQALP"
     gene            complement(2120..4201)
                     /locus_tag="VFA35_03855"
     CDS             complement(2120..4201)
                     /locus_tag="VFA35_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HZT55337.1"
                     /translation="MNTTIVRAACPHDCPDTCAIRVTVEDGRAIKVQGDPDHPPTHGA
                     LCTKVSRYPERTYHAERVLHPMKRVGPKGGGRFARVSWDEALADIASRLKAIAARDAQ
                     AIVPYSYAGTMGLLQGESMAARFFNKLGASVLDRTICSSAGGEALAATYGAKVGMHLE
                     FYAESRLIVIWGSNSIASNLHFWTFAQAAKRRGAKLICIDPRRTETADKCHQHIALLP
                     GTDGALALGLMHELIVHNWLDHDYIADHVDAAGWPALRERALQWPPERVAAVCGITAD
                     EVRTLARDYGSTRPVAIRMNYGMQRVRGGGNATRLIALLPALVGAWRQRGGGLLLSSS
                     GWFRGVRNDAALQRPDLLNGRTPRTINMSTIGDDLLRASSAAFGPKIEALIVYNSNPV
                     AVAPESPKVVRGFARDDLFTVVLEHFMTDTADHADYVLPATTQLEHLDLHTSYGHTYV
                     MLNEPAIAPLGEAKPNTQIFRELAARMGFDEPCFADDDEALARAAFNDPIDLVALRAT
                     GWFKLTVADAPFAAGNFPTADGRCIIDSVAHGVPDHVPNHESAASAPELAQRFPLAMI
                     SPPARNFLNSSFVNVKSLRDIEGEPLVEIHAADASGRGIVDGAMVRVFNDRGSYLCRA
                     RISRRARPGVVNGLGVWWRKLGAGGSNVNELTHQRLTDIGRAASFYDCLVDVQPADAG
                     AGEVRSADAVS"
     gene            4374..>5458
                     /locus_tag="VFA35_03860"
     CDS             4374..>5458
                     /locus_tag="VFA35_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT55338.1"
                     /translation="MKRRDAIKWLAGSSAALALPALAQTNRIVLGQSAAFSGPAAQLG
                     IQMNMGAKIYFAALNAGGGVNGSAIELRTLDDGYEPDRCKANTEKFIKDEVFGLFGYV
                     GTPTCLAALPLVVDAKIPFFGPFTGAEALRDPFHKSVFHLRASYYDETALIVKQLTSL
                     GLKKIAVFYQNDAYGKAGLEGVNRALKAQDLVPIALGTVERNTVNVAQAVKDITAKMP
                     DAVVQISAYKSCAAFIREARKAGYGGTFFNVSFVGTQALADELGKEGRGIMVSQVMPF
                     PFSTTTAISREYLDAVRKAGGDAQPNYSSMEGYLAAKVLTEGLKRAGRNASRDSLVSA
                     LEWIQATAFGGFNVNFGPRQHVASRFV"
BASE COUNT          857 a         1868 c         1875 g          858 t
ORIGIN      
        1 agccgctgtc gttggcgagg ttgccgaacg agtccttgcc gaatgtggcc tcgctcatcc
       61 cgcgtcggta cccgtagagg tactggatac cgcgcccgtc gctgttcatc gcaatcaatt
      121 cgccagtggc aagaggctcg cccaccgtcc cgaactcgcg acccaccatg gtgacgatgc
      181 gcgtatcggt cacccaggca tgccgcagcg gaagatcgcc cgcctgcgag cggaccgcgc
      241 tcagcagctt catcccgtcg agctgataga cgaccgtcgc gtactcgtca cgcggctcct
      301 tcggcgtgcg ctgggtgacg acgagcagct tcccgtcggg tgacatgacc ggcgcgaaga
      361 agttgccgcg ccgcacgaag tcggcagtcg ggatcggcgg ggcggccgcc aggctcgcgc
      421 cgcagagcgc ggcgagcagc gccggcaccc agcgcggcgc gcagccggtg gccggccgac
      481 aggggttggg caatgcgaga agggtgggca tcgcggcagg actcctcgaa aaggggaagg
      541 gaaagttcgg atcgcgctcg tgccgcatgc ggtcgggcgc aggttcaacg gcaccatcgc
      601 gcggatccgc ctgcccgcgg gatcgtcgcc ctacttgcgc gggtacgcgc cgagcgcgcc
      661 ggccccgcga atctctcaaa atgggcgtca cctcttggat gtcttcgccg ccgccttctt
      721 ggtggccggc ttgctcttgg cgagcaacgc atcgaggttc ttctcgatct cggccacgat
      781 ggtcttgctg aaggccccga acaggaaacc cagcttcgca tccaccgtga accggtgcgc
      841 ctcgacgagc agttcgcccg tcacacccga gcgcgtgaac tccaccgtgt cgctggtctt
      901 gccttcgacg atggtgcatg ccatcccgaa cttctcttcg gcatcgatgg cccattgcag
      961 cgcgaccttg cgggccttgg cgaggccaag tttgtgttcg cgttggatgt ggatgtctgg
     1021 catgtcgttt ttcctcaggg caatgcttgg cggcgttcgg ccagcggcag agccgcgagc
     1081 cgccggacct cggcgtagaa gcgcgtgaag tcgcgccctt cgcgctcgaa caggcgctcg
     1141 aaattcggca ccagttcgtt gtacgcggcg agcacgccga aggacgcatt gttggcgcgc
     1201 gcaagccagc cgtcataccc ggaatacccg ccccagcgcg cgcgcttcgt cgcatcgaac
     1261 tcggcgcgca actcgcgcat cagcgcggcc ttgccggcgc gcttgtcggc gtcgctcgcc
     1321 gggctgtcgt agagcacatc gaagcgcgca cggtagcgcg cggtcagctc gcgaaagtcg
     1381 cgccggcgcg acgcaacctg ttcgtactcg tcacgcgcct gcgagctcgc gtgcgtggcc
     1441 agccagcgcg cgctgccgat gcgctcgacg gcggtcgcga acgattcgtt gaacaccgtg
     1501 tcgcctttcg cgaacgcgat ctggtgcgcg agctcatgga agatcagccg cgcgagctcg
     1561 ccttcggtat cgtcgatgaa ggtgttcagc agcgggtcgg caagagcatc accgggcagc
     1621 aggccgagcg tcgaatatgc ggggacggcg tagacgctca cctccagccc gagcggcttc
     1681 aggctcgcgg cgaacgcgtt cgcctcggcc gggtcgtagt agccgcggta gccgacgcag
     1741 ccgacgaccg ggaagcacca ggtctgcagg cgcaggctca attcgggcgc tgccaccacg
     1801 ttccagaccg ctgctccgcg cctcaggtcg gcgtagcggc ggtagctcgc gttgtcgggc
     1861 tccttcaact cgctgaccgc gaagtcgcgg atgcgctggc tgagttcgag acgcgcgcgc
     1921 aagtccgcgc tcgtgtgcgg atcggccagc cactcgggca ccggcctggc cgcgcgcacg
     1981 atggccaagt gcccgccgac cgattgcgcg tagtagccga tcgtgctgca gcccgaggcc
     2041 agacacaccg tggccgccgc cagcaccgcg acgccgagcg caccgagaaa ggccatgccc
     2101 agaatcagcc agccgcgctt cacgacacgg cgtcggccga ccggacctcg cctgcaccgg
     2161 cgtcagccgg ctgcacgtcg accaggcagt cgtagaagct ggcggcgcgg ccgatgtcgg
     2221 tgagccgctg gtgcgtcagt tcgttgacgt tgctgccgcc ggcaccgagc ttgcgccacc
     2281 acacacccag gccgttgacg acgccgggtc gcgcacgccg gctgatgcgc gcacggcaca
     2341 ggtagctgcc gcggtcgttg aacacgcgca ccatcgcgcc gtcgacgatg ccgcgcccgc
     2401 tggcatcggc cgcgtggatc tcgaccagcg gctcgccctc gatgtcgcgc aggctcttga
     2461 cgttgacgaa gctggagttc aagaagttgc gcgctggcgg cgagatcatc gccagcggaa
     2521 agcgctgcgc gagttcgggc gcgctcgcag ccgactcgtg gttcggcacg tgatcgggca
     2581 cgccgtgcgc cacggagtcg atgatgcagc ggccgtcggc ggtcgggaag ttgcctgcgg
     2641 cgaacggcgc gtcggccaca gtcaacttga accagccggt cgcgcgtagc gcgacgagat
     2701 cgatcgggtc gttgaacgca gcgcgcgcga gcgcttcgtc gtcgtcggca aagcagggtt
     2761 cgtcaaagcc catgcgcgcc gcgagctcgc gaaagatctg tgtgttcggc ttggcctcgc
     2821 cgagcggcgc gatcgcgggc tcgttcagca tcacgtaggt gtggccgtag ctggtgtgca
     2881 ggtcgaggtg ctcgagttgc gtggtcgcgg gcagcacgta gtcggcgtgg tcggcggtgt
     2941 cggtcatgaa gtgttcgagc acgacggtga acaggtcgtc gcgcgcaaag ccgcgcacca
     3001 ccttcggcga ctcgggcgcc accgcgaccg ggttgctgtt gtagacgatc agcgcttcga
     3061 tcttcggccc aaacgctgcc gacgacgcac gcagcagatc gtcaccgatc gtgctcatgt
     3121 tgatcgtgcg cggcgtcctg ccgttcagca gatcgggccg ctgcaacgcg gcgtcgttgc
     3181 gcaccccgcg aaaccagccc gagctcgaca acagcagccc gccaccgcgc tggcgccagg
     3241 cgccgaccag cgccggcagc agcgcgatca gccgcgtcgc gttgccgccg ccgcgcacgc
     3301 gctgcatgcc gtagttcatg cggatcgcca ccgggcgcgt gctgccgtag tcgcgcgcca
     3361 gcgtgcgcac ctcgtcggcc gtgatgccgc agaccgcggc gacgcgctcg ggcggccact
     3421 gcagcgcgcg ctcgcgcaac gccggccagc cggccgcgtc gacatggtcc gcgatgtagt
     3481 cgtggtcgag ccagttgtgg acgatcaact cgtgcatcag cccgagcgcg agcgcgccgt
     3541 cggtgcccgg cagcagcgcg atgtgctggt ggcacttgtc ggcggtctcg gtgcggcgcg
     3601 ggtcgatgca gatcagcttg gcgccgcggc gcttggcggc ctgcgcgaag gtccagaaat
     3661 gcaggttcga cgcgatcgag ttgctgcccc agatcacgat caatcggctc tcggcgtaga
     3721 actcgaggtg catgccgacc ttggcgccgt aggtcgcggc gagcgcttcg ccgccggcgc
     3781 tcgagcagat cgtgcggtcg agcaccgagg cgccgagctt gttgaaaaag cgcgccgcca
     3841 tgctctcgcc ctgcagcaag cccatcgtgc cggcgtagct gtagggcacg atcgcttgcg
     3901 cgtcgcgcgc cgcgatcgcc ttcaggcgtg acgcgatgtc ggccagcgct tcgtcccagc
     3961 tcacgcgggc gaaccggccg cctcctttcg ggccgacgcg cttcatcggg tgcagcacgc
     4021 gctcggcgtg ataagtgcgc tccgggtagc gcgagacctt ggtacacaat gccccgtgcg
     4081 tcggtgggtg gtcggggtcg ccctgcacct tgatcgcgcg gccgtcctcg acggtgacgc
     4141 ggatcgcgca ggtgtcgggg caatcgtgcg ggcaggcggc gcggacgatg gtcgtgttca
     4201 taggtagggg tgaactattg cacaccgagg cagcgcacaa tcgtatgcgt cgggccacag
     4261 ccgcgtgacg cgggccgtgc cagcctctat gctgcgcgca tgccgggaaa caccggacgg
     4321 gcccggcgcg cgagcgccgg cggcgacccc gacacatcat cgttgaggaa tccatgaaac
     4381 gtcgtgacgc catcaagtgg ctggccggat cgagcgccgc actcgcgctg cccgcgctgg
     4441 cccagaccaa ccgcatcgtg ctgggccaat cggccgcgtt cagcgggccg gccgcgcagc
     4501 tcggcatcca gatgaacatg ggcgcgaaga tctacttcgc cgcactcaac gctggcggcg
     4561 gcgtcaacgg cagcgcgatc gagctgcgca cgctcgacga cggctacgag cccgaccgct
     4621 gcaaggccaa caccgagaag ttcatcaagg acgaggtctt cggtttgttc ggctatgtcg
     4681 gcacgcccac ctgcctggcg gcgctgccgc tggtggtcga cgcgaagatc ccgttcttcg
     4741 gcccgttcac cggcgccgag gcgctgcgcg atccgttcca caagtcggtg ttccatctgc
     4801 gcgcgtcgta ctacgacgag accgcgctga tcgtcaagca gctcacctcg ctcggcctga
     4861 agaagatcgc ggtcttctac cagaacgacg cctacggcaa ggccggcctc gaaggcgtga
     4921 accgcgcgct gaaggcacag gacctcgtgc cgatcgcgct cggcacggtc gagcgcaaca
     4981 ccgtcaacgt cgcgcaggcg gtcaaggaca tcaccgcgaa gatgcccgac gcggtggtgc
     5041 agatcagcgc ctacaagtcg tgcgccgcct tcatccgcga ggcccgcaag gccggctatg
     5101 gcggcacctt cttcaacgtc tcgttcgtcg gcacgcaggc gctcgccgac gagctcggca
     5161 aggaaggccg cggcatcatg gtcagccagg tgatgccgtt cccgttctcg accacgaccg
     5221 cgatctcgcg cgagtacctc gacgcggtgc gcaaggccgg cggcgacgcg cagccgaact
     5281 actccagcat ggagggctac ctcgccgcca aggtgctaac cgagggcctg aagcgcgccg
     5341 gccgcaacgc gagccgcgac agcctggtgt cggcgctgga gtggatccag gccaccgcct
     5401 tcggcggctt caacgtgaac ttcggcccca ggcagcacgt cgcctcgcgt ttcgtcga
//
LOCUS       DASRDG010000232         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_36939, whole genome shotgun sequence.
ACCESSION   DASRDG010000232 DASRDG010000000
VERSION     DASRDG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36939"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1950
                     /locus_tag="VFA35_03865"
     CDS             <1..1950
                     /locus_tag="VFA35_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZT55339.1"
                     /translation="AGVTFLAAEGGEEDIRLRGFSLAASGDIYVDSLRDPAFYERDTF
                     SFDRIELLRGSASMLFGRGSTGGVVNQVSKQAFLANASQVDFTVGDGSYLRFTGDFNL
                     KTSETSAARINVMTTNASGGGGNDKLDKQGLAPTFRWGIGTPDEFYVSGYYLRNNNGI
                     NYGLPWLRASSTQASANPSGIIEGLDPKNYYGAASDYNAGGAALATAGWTHRFADGGE
                     LKSAIRQGRYDRDQRAGTIRFTNPTGADNISDATLLTRGSQNKIQNMLTTYAQSDYSK
                     QFDWFGVKNEVLTGVDLAHEVFKGFAAVLPAGVVLDKNTPRTTIGTPDDGTGWVDESK
                     RLVRQQAAFDAKSLGIYFQDLVQITPTWKLLGGLRWDYFKGSYQTFQTAAGATVPVGT
                     VTADRGRSDNLFSHRLGALYQPNDRMSFHFSYGTSFNTSGDTYQYDAPGSNTPPEGSR
                     NFELGAKLDLLEGKLSTRLALFHSTKFNERNRDSPDGTPLTDYVLSGRRHTAGVELDL
                     AGRITPKWEVFGSYAWTPIAKIDVGPPSGVALSGERVGDRPSLTPRHSGTIWTTYQLL
                     TSLRLGAGLNARSSQTPNRNPPGIVAPSFVTGDLLAEYTLSEAVAFKLNVNNVTNKLY
                     ADSLYTGHYIPGPGRKVQVTMTARF"
     gene            1961..2650
                     /locus_tag="VFA35_03870"
     CDS             1961..2650
                     /locus_tag="VFA35_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HZT55340.1"
                     /translation="MLLHVKDVHNADELREARAILKGATWGDGRVTAGVQSAQAKNNE
                     QLPQDGAETKALQRIVLGGLNRHAVFFSAALPRRVFPPLFNRYGGASNSFGDHVDNAI
                     RFLPGGHGERLRTDVSCTLFLADPHEYDGGELIVEDSFGAQRIKLPAGDMVLYPGTSV
                     HRVEPVTRGHRLASFFWLESMVRSSEQRRLLFDMDSHLMRLRASVGETDPAVIGLTGT
                     YHNLLRLWADA"
     gene            2683..2949
                     /locus_tag="VFA35_03875"
     CDS             2683..2949
                     /locus_tag="VFA35_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZT55341.1"
                     /translation="MKRQLLTLCLALAAAGAFAQHAEKCAPIPKEEWKPQAELERKLK
                     NEGWTISRVKIENGCYEVYGKNATGKKMETFFHPKTLEVVTAPK"
     gene            2963..3514
                     /locus_tag="VFA35_03880"
     CDS             2963..3514
                     /locus_tag="VFA35_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZT55342.1"
                     /translation="MHAEPAPTVRVWDRAVRALHWTLVGAVALSALGLIALFGVHRPA
                     GYVALAVVLVRAGWGAVGGRYARFAQFVRGPRAALRYARAVAARREPRFIGHNPLGGW
                     MVVALMSCVVALAASGWLYTTDAFWGDETVETIHRALAWTLLGLVVAHVAGVLFTSLR
                     HRENLVAAMFSGRKRAPARDDIA"
     gene            3716..>3921
                     /locus_tag="VFA35_03885"
     CDS             3716..>3921
                     /locus_tag="VFA35_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55343.1"
                     /translation="MATAKKAPAKKAAPAKKAAPAKKAAAKKAAPAKKAAPAKKAAPA
                     KKAAPAKKRTPNAAFMKAMTPSAAL"
BASE COUNT          688 a         1395 c         1295 g          543 t
ORIGIN      
        1 gccggcgtga ccttcctcgc ggccgaaggc ggcgaggaag acatccgcct gcgcggcttc
       61 tcgctcgcgg ccagcggcga catctacgtc gacagcctgc gcgacccggc cttctacgag
      121 cgcgacacct tcagcttcga ccgcatcgag ctgctgcgcg gctcggcctc gatgctgttc
      181 gggcgcggct cgaccggcgg cgtggtgaac caggtcagca agcaggcgtt tctggcgaac
      241 gcgagccagg tcgatttcac ggtcggcgac ggcagctacc tgcgcttcac cggcgacttc
      301 aacctgaaga cgagcgagac ctcggcggcg cgcatcaacg tgatgaccac caatgccagc
      361 ggcggcggcg gcaacgacaa gctcgacaag cagggcctgg cgccgacctt ccgctggggc
      421 atcggcacgc ccgacgagtt ctacgtctcg ggctactacc tgcgcaacaa caacggcatc
      481 aactacggcc tgccgtggct gcgcgcgagc tcgacccagg ccagcgcgaa cccgtccggc
      541 atcatcgaag ggctggatcc gaagaactac tacggcgccg cgagcgacta caacgccggc
      601 ggcgcggcgc tcgcgaccgc cggctggacg caccgcttcg ccgacggcgg cgagctcaag
      661 tcggcgatcc gccaaggccg ctacgaccgc gaccagcgcg ccggcacgat ccgcttcacc
      721 aaccccaccg gcgcggacaa catcagcgac gcgacgctgc tgacgcgtgg atcgcagaac
      781 aagatccaga acatgctgac gacgtacgcg cagagcgact acagcaagca gttcgactgg
      841 ttcggcgtga agaacgaggt gctgaccggc gtcgacctcg cgcacgaggt gttcaagggc
      901 tttgctgcgg tgctgccggc cggcgtcgtg ctcgacaaga acacgccgcg cacgacgatc
      961 ggcacgccgg acgacggcac cggctgggtc gacgaaagca agcgcctggt gcgccagcag
     1021 gccgccttcg acgccaagtc gctcggcatc tactttcagg acctggtgca gatcacgccg
     1081 acctggaagc tgctcggcgg cctgcgctgg gactacttca agggcagcta ccagaccttc
     1141 cagaccgccg ccggcgcgac cgtgccggtc ggcaccgtga cggccgatcg cggccgctcc
     1201 gacaacctgt tcagccaccg cctcggcgcg ctgtaccagc cgaacgaccg gatgtcgttc
     1261 cacttctcgt acggcacctc gttcaacacc tcgggcgaca cctaccagta cgatgcgccg
     1321 ggctcgaaca ccccacccga aggcagccgc aacttcgagc tgggcgccaa gctcgacctg
     1381 ctcgaaggca agctgagcac gcgcctggcg ctgttccact cgaccaagtt caacgagcgc
     1441 aaccgcgact ccccggacgg cacgccgctg accgactacg tgctctcggg ccgccgccac
     1501 accgcgggcg tcgagctcga cctcgccggc cgcatcacgc cgaagtggga ggtgttcggc
     1561 tcgtacgcat ggaccccgat cgcgaagatc gacgtcggcc cgccgagcgg cgtggcgctg
     1621 tcgggcgagc gcgtcggcga ccggccgtcg ctgacgccgc gccacagcgg cacgatctgg
     1681 accacctacc agctgctgac cagcctgcgc ctgggcgccg gcctgaacgc acgcagctcg
     1741 cagacgccga accgaaaccc gccgggcatc gtcgcgccga gcttcgtcac cggcgacctg
     1801 ctggccgagt acacgctgag cgaggccgtc gcgttcaagc tcaacgtcaa caacgtgacc
     1861 aacaagctgt atgccgattc gctctacacc ggtcactaca tccccggccc gggtcgcaag
     1921 gttcaggtca cgatgaccgc gcgcttctag aagaaggccg atgctgctgc acgtcaagga
     1981 cgttcacaac gccgacgaat tgcgcgaggc gcgcgccatt ctgaagggcg cgacctgggg
     2041 cgacggccgg gtcacggccg gcgtgcaatc ggcgcaggcc aagaacaacg agcagctgcc
     2101 gcaggacggc gccgagacga aggcgctgca gcgcatcgtg ctcggcggcc tgaaccggca
     2161 tgcggtgttc ttctcggcgg cgctgcccag gcgcgtgttc ccgccgctgt tcaaccgcta
     2221 cggcggcgcg agcaattcgt tcggcgacca tgtcgacaac gcgatccgct tcctgcccgg
     2281 cggacacggc gagcgcttgc gcaccgacgt cagctgcacg ctgttcctcg ccgacccgca
     2341 cgagtacgac gggggcgagc tgatcgtcga ggacagcttc ggcgcgcagc gcatcaagct
     2401 gccggccggc gacatggtgc tgtaccccgg caccagcgtg catcgggtcg agccggtgac
     2461 gcgcggccac cggctcgcga gcttcttctg gctcgagagc atggtgcgca gcagcgagca
     2521 gcgccgcctg ctgttcgaca tggactcgca cctgatgcgc ctgcgcgcgt cggtcggcga
     2581 gaccgatccc gcggtgatcg gcctgaccgg cacgtatcac aacctgctgc ggctctgggc
     2641 cgacgcctga gcgcagcttt ttcaccgctg aaaggaagcc cgatgaaacg ccaactcctg
     2701 accctgtgcc tggccctggc cgccgccggc gcgttcgcgc agcacgccga gaagtgcgcg
     2761 ccgatcccga aggaagagtg gaagccgcag gccgagctcg agcgcaagct gaagaacgag
     2821 ggctggacga tcagccgcgt caagatcgag aacggctgct acgaggtcta cggcaagaac
     2881 gcgaccggca agaagatgga gaccttcttc catcccaaga ccctcgaggt cgtgaccgcg
     2941 ccgaagtgac cggcccccgc cgatgcacgc cgagcccgca ccgaccgtgc gcgtctggga
     3001 ccgcgcggtc cgcgcgctgc actggacgct ggtcggcgcg gtcgcgctct cggcgctcgg
     3061 cctgatcgcg ctgttcggcg tgcaccggcc ggccggctac gtggcgctcg cggtggtgct
     3121 ggtgcgcgcc ggttggggcg cggtcggcgg ccgctacgcg cgcttcgcgc agttcgtgcg
     3181 cgggccgcgc gccgcgctgc gctacgcgcg cgcggtggcg gcgcgacgcg agccgcgctt
     3241 catcggccac aacccgctcg gcggctggat ggtcgtcgcg ctgatgagct gcgtcgtcgc
     3301 gctcgccgcg agcggctggc tctacacgac cgatgcgttc tggggcgacg agaccgtcga
     3361 aacgatccat cgtgcgctcg cgtggacgct gctcggcctc gtcgtcgcgc acgtcgccgg
     3421 cgtgctcttc acgagcctgc ggcaccgcga gaacctggtc gcggcgatgt tcagcggccg
     3481 caagcgggca ccggcgcgag acgacattgc ctgagcgcag cacgaggcgg tgacgcctgg
     3541 ccacgacaga gcgctgtcta cctcggagaa ttgcgcgttt ctccagtgaa atcttcttct
     3601 ctttgcccta tgcaggcatt accattcgct ccgcacggtg ctatggagaa atgagagtcc
     3661 acgcggctct cgcccatccc gggcacatca accactcttg atggagagaa tcaacatggc
     3721 aactgcgaag aaagcgccgg caaagaaggc ggcaccagcg aagaaggccg ctccggccaa
     3781 gaaggcggct gcaaagaagg ccgcgcctgc caagaaggca gcgcccgcca agaaggcggc
     3841 tcctgcgaag aaggcggctc cggccaagaa gcgcactccc aacgcggcct tcatgaaggc
     3901 catgaccccg agcgccgcac t
//
LOCUS       DASRDG010000233         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1641362, whole genome shotgun sequence.
ACCESSION   DASRDG010000233 DASRDG010000000
VERSION     DASRDG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641362"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..653
                     /locus_tag="VFA35_03890"
     CDS             300..653
                     /locus_tag="VFA35_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZT55344.1"
                     /translation="MTHYLLTIYQPDGGTPPPEFLARVGRDLHALNQEIKAAGAWVFS
                     RGLHAPSTATVVRARGREVLTTDGPYAEGKEHVGGFWIVNARDLDQALDWARKAARAT
                     TLPIEVRPFQDVAEH"
     gene            662..1888
                     /locus_tag="VFA35_03895"
     CDS             662..1888
                     /locus_tag="VFA35_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HZT55345.1"
                     /translation="MADANPAAIEALFRAEYGRAVAALARTCGDLDAAEEAVADAFAI
                     ALQRWPADGLPPSPIGWIVATARNRAIDRRRRALARPVGDEPVELLHARAAAPVDEVL
                     PDDLLRLVFTCCHPALARVSQVALTLRLCAGLSTAAIARAFLVPEPTMAQRLARAKGK
                     IRDARIPYRVPAAAELPERLPPVLAVVYLVFNEGHTASAGSGLVRGELCDEAIRLARL
                     LAALMPDEAEVQGLLALLLLVDSRRAARTDAHGAFVPLAEQDRSRWDRARIAEGQELL
                     RGCLRRDRPGPYQLQAAIQAAHGDARSAVATDWRQIVQLYDHLLALDPGPVVALHRAV
                     AVAEVDGAPAGLALVDALAVELDGHHLLHAIRADLLRRLDRDAEAARAYARAIDLTAN
                     EVEREFLRKRSESLAR"
     gene            complement(1974..>2154)
                     /locus_tag="VFA35_03900"
     CDS             complement(1974..>2154)
                     /locus_tag="VFA35_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55346.1"
                     /translation="ERATGRERENDWLTAADVRLFSGCYGSTRFQLGYAYAFDTIRDG
                     ERGGGEFVVQVTGRF"
BASE COUNT          276 a          806 c          791 g          281 t
ORIGIN      
        1 caaggcgcgg tgaccgcgcc cgccgcgcga atccacggtt gattccacac ctggacgcgc
       61 ggcaactccc gctgccatgg cagcgggagc gacgttctcc acgccgcacc tgcgctatcc
      121 acaacgtgga gaggctgtgg aatcgggccg gcgcgcgctg cggacgcgac gatgtcgaat
      181 cgggatgtcg aatcgcggcg gtgccgttcg tgttgcggtc ggcggccgcc gcatgacggg
      241 tcgcggcgaa cccgcagccg tcggaccggc gcccgccctt tcgccaacga gacgaaccga
      301 tgactcacta cctgctcacc atctaccagc ccgacggcgg gacgccgccg ccggagttcc
      361 tggccagggt cggccgcgac ctgcacgcgc tcaaccagga gatcaaggcc gccggcgcct
      421 gggtgttcag ccgcggtctg cacgcgccga gcaccgcgac ggtcgtgcgc gcgcgcggcc
      481 gcgaagtgct caccaccgac ggtccgtacg ccgagggcaa ggagcacgtc ggcggcttct
      541 ggatcgtgaa cgcgcgggat ctcgaccagg cgctcgactg ggcgcgcaag gccgcgcgcg
      601 cgacgacgct gccgatcgag gtgcggccgt tccaggacgt cgccgagcat tgagccgcgc
      661 catggccgac gcgaatcctg ccgcgatcga agcgctgttc cgcgccgagt acggccgcgc
      721 ggtcgcggcg ctggcgcgga cctgcggcga cctcgacgcc gccgaggagg cggtcgccga
      781 cgcgttcgcg atcgcgctgc agcgctggcc ggccgacggc ctgccgccga gtccgatcgg
      841 ttggatcgtc gcgaccgcgc gcaatcgcgc gatcgaccgc cggcggcgcg cgttggcgcg
      901 gccggtcggc gacgaacccg tcgaactgct gcacgcgcgc gcggccgcgc ccgtcgacga
      961 agtgctgccc gacgacctgc tgcggctcgt cttcacgtgc tgccatcccg cgctcgcgcg
     1021 cgtgtcgcag gtggcgctga cgctgcggct ctgcgccggg ctgtcgaccg cggcgatcgc
     1081 gcgcgcgttc ctcgtgcccg agccgacgat ggcgcagcgg ctcgcgcgcg ccaagggcaa
     1141 gatccgcgac gccaggatcc cgtaccgcgt gcccgccgcg gccgagctgc ccgaacggct
     1201 gccgccggtg ctcgcggtcg tctacctggt gttcaacgaa ggccacaccg cgagcgcggg
     1261 cagcggtctc gtgcgcggcg aactgtgcga cgaggcgatc cggctcgcgc gcctgctcgc
     1321 ggcgctgatg cccgacgaag ccgaggtgca ggggctgctc gcgctgctgc tgctcgtcga
     1381 ttcgcggcgc gcggcgcgca ccgacgcgca cggcgcattc gtgccgctcg ccgagcagga
     1441 tcgttcgcgc tgggaccgcg cccgcatcgc cgaaggccag gaactgctgc gcggctgcct
     1501 gcgccgcgac cggcccgggc cgtaccagct gcaggccgcg atccaggccg cgcacggcga
     1561 cgcgcgttcg gcggtggcga ccgactggcg gcagatcgtg cagctctacg accacctgct
     1621 cgccctcgac ccgggtccgg tcgtcgcgct gcaccgcgcg gtcgcggtcg ccgaggtcga
     1681 cggtgctccg gcggggctcg cgctcgtcga cgcgctcgca gtcgagctcg acggccatca
     1741 cctgctgcac gcgatccgcg ccgacctgct gcgccggctc gatcgcgacg ccgaggccgc
     1801 gcgggcctac gcgcgcgcga tcgacctgac ggcgaacgag gtcgagcgcg agttcctgcg
     1861 caagcgcagc gaatcgctcg cgcgctgact ccttcgcgcg gtcgatgcgg gaacgcatgc
     1921 cgtgactgtg tccgaaccgc ggcgcgcgtt gcagtccccg cgcggattcg cgctcagaag
     1981 cggccggtga cctgcacgac gaactcgccg ccgccgcgct cgccgtcgcg gatcgtgtcg
     2041 aaggcgtagg cgtagccgag ctggaagcgc gtgctgccgt agcagcccga gaacaggcgc
     2101 acgtcggcgg cggtgagcca gtcgttctcg cgctcgcggc cggtcgcgcg ctcg
//
LOCUS       DASRDG010000234         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1547326, whole genome shotgun sequence.
ACCESSION   DASRDG010000234 DASRDG010000000
VERSION     DASRDG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547326"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFA35_03905"
     CDS             complement(<1..466)
                     /locus_tag="VFA35_03905"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HZT55347.1"
                     /translation="MTPNNLPLRTTLDAALHQIRATLTAVAARLAEKPGAMWPSGGGT
                     GERDLLNSAQSDLRRNIGVLNQVFADELGRRIGQELSPRVDAKRTLAAASWQTLSLVD
                     DSEMEERMYADRIAQQISHACEPELRELAAYMGALLETGRADQDRNPLRAEVL"
     gene            complement(617..1048)
                     /gene="mscL"
                     /locus_tag="VFA35_03910"
     CDS             complement(617..1048)
                     /gene="mscL"
                     /locus_tag="VFA35_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZT55348.1"
                     /translation="MSFASEFKEFALKGNVVDLAVGVIIGAAFGKIVDSLVKDLIMPI
                     VGRVFGGLDFSNYFVALASPPANYNGPMTYEALTKAGVPLFAYGNFITVAINFVILAF
                     IIFMMVKQINRLKLNEALAPAAAAPPEDVLLLREIRDSLKK"
     gene            1213..1815
                     /gene="petA"
                     /locus_tag="VFA35_03915"
     CDS             1213..1815
                     /gene="petA"
                     /locus_tag="VFA35_03915"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZT55349.1"
                     /translation="MSDQQIDSSKRTWIIASSCAGAVGAGFAAVPFVSSFQPSERARA
                     AGAAVEADISGLKPGEKMTVEWRGKPVWIVRRTEAQLAELPKLDKELADPASKRTNFA
                     STPEYARNEWRSIKKEYLVVVGICSHLGCSPSDKFVPGPQPSLPNDWEGGFLCPCHGS
                     TFDMAGRVFKDKPAPDNLEVPPYMYLSDTRLIIGEDNKKA"
     gene            1878..>2333
                     /locus_tag="VFA35_03920"
     CDS             1878..>2333
                     /locus_tag="VFA35_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZT55350.1"
                     /translation="MAEYHEAPANATAARKLLNWVDNRFPLSKLYNEHMGEYYAPKNF
                     NFWYIFGSLAMLVLVIQIVTGIFLVMHYKPDAAQAFASVEYIMRDVPGGWIIRYMHST
                     GASAFFIVVYLHMFRGLMYGSYRKPRELVWIFGCGIFLCLMAEAFMGYLL"
BASE COUNT          401 a          763 c          778 g          391 t
ORIGIN      
        1 cgagcacttc ggcgcgcagc gggttgcggt cctggtcggc ccgtcccgtc tcgagcagtg
       61 cgcccatgta ggcggcgagc tcgcgcaact cgggttcgca ggcgtgtgag atctgctggg
      121 cgatgcggtc ggcgtacatc cgctcttcca tctcgctgtc gtcgaccagg ctcagggtct
      181 gccagctggc ggcggcgagc gtgcgcttgg cgtccacgcg cggcgagagt tcctggccga
      241 tgcgacgacc gagctcgtcg gcgaacacct ggttgaggac gccgatgttg cggcgcagat
      301 cggactgggc cgagttcagc aggtcgcgct cgccggtgcc accgccggat ggccacatcg
      361 cgcccggttt ctctgccagg cgggcggcca ctgccgtcag cgtcgcccgg atctggtgga
      421 gcgccgcgtc gagcgtggtt cgcaacggga ggttgttcgg ggtcatctcg gcgtggttgg
      481 cgcaccgtca ccggcgcgag cggcgtcgcc accagcgtag cgcacgcccg ccaagtatga
      541 gcccgaaaat gcggctgaaa agtcgcgctg ctcgggcgct tgcgcgcgac gcaacagcga
      601 ccgctcccga agcgcgctac ttcttcagcg aatcgcggat ttcgcgcaac agcagcacat
      661 cttccggcgg cgcggcggcg gcgggcgcga gcgcttcgtt cagcttcagg cggttgatct
      721 gcttgaccat catgaagatg atgaaggcca ggatcacgaa gttgatcgcg accgtgatga
      781 agttgccgta ggcgaacagc ggcacgccgg ccttggtgag cgcctcgtag gtcatcggcc
      841 cgttgtagtt ggcggggggg ctcgccagcg cgacgaagta gttcgagaag tcgaggccgc
      901 cgaagaccct gccgacgatc ggcatgatca ggtccttgac gagcgagtcg acgatcttgc
      961 cgaatgcggc gccgatgatc acgccgaccg cgaggtcgac cacattgccc ttcagggcga
     1021 actctttgaa ctcggacgcg aacgacatgg gtacggctcc tgtgggatgg cgaggacagc
     1081 cagggcccgc atgcggtggc tgcgggcggg ctgccgcgta gtattccgga ggtcgcgcgc
     1141 aagtcaagtg ccttgacacg tccgctagaa tgccgggttg accgtgaccg aagccaagtt
     1201 ccgaggaaac ctatgagtga ccagcagatc gacagcagca agcgcacctg gatcatcgct
     1261 tccagctgcg ccggtgccgt cggtgcaggc ttcgcggccg ttccgttcgt cagcagcttc
     1321 cagccgtcgg agcgcgcgcg cgctgccggg gcggccgtcg aagccgacat cagcggcctg
     1381 aagcccggcg agaagatgac cgtcgaatgg cgcggcaaac ccgtgtggat cgtgcggcgc
     1441 accgaagcgc agctcgccga actgcccaag ctcgacaagg aactggccga cccggcctcc
     1501 aagcgcacca acttcgcgag cacgcccgag tacgcgcgca acgagtggcg ttcgatcaag
     1561 aaggagtact tggtcgtcgt cggcatctgc agccacctcg gctgctcgcc gagcgacaag
     1621 ttcgtgccgg gcccgcagcc ctcgctgccg aacgactggg agggcggctt tctgtgcccg
     1681 tgccacggct cgaccttcga catggccggc cgcgtcttca aggacaagcc cgcccccgac
     1741 aacctcgaag tgccgccgta catgtacctg tcggacaccc ggctgatcat cggcgaagac
     1801 aacaagaaag cctgagcgag cgccgcgcgc gacgctgcat ccaggtacca agacggcacc
     1861 ccaggagtac ccccgacatg gccgaatacc acgaagcccc cgcgaacgcg acggcggcgc
     1921 gcaagctgct gaactgggtc gacaaccggt tcccgctctc caagctctac aacgagcaca
     1981 tgggcgagta ctacgccccg aagaacttca acttctggta catcttcggc tcgctcgcga
     2041 tgctggtgct ggtgatccag atcgtgaccg gcatcttcct cgtgatgcac tacaagccgg
     2101 atgcggcgca ggcgtttgca tcggtcgagt acatcatgcg cgacgtgccc ggcggctgga
     2161 tcatccgcta catgcactcg accggcgcga gcgcgttctt catcgtcgtc tacctgcaca
     2221 tgttccgcgg gctgatgtac ggctcgtacc gcaagccgcg cgagctggtc tggatcttcg
     2281 gctgcggcat cttcctgtgc ctgatggccg aggccttcat gggctacctg ctg
//
LOCUS       DASRDG010000235         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_961397, whole genome shotgun sequence.
ACCESSION   DASRDG010000235 DASRDG010000000
VERSION     DASRDG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961397"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VFA35_03925"
     CDS             <1..1324
                     /locus_tag="VFA35_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55351.1"
                     /translation="FDPRPVVADSRLSYDKPIAFSWLASPFVAALGNHHGLMAVSLLG
                     ALAMLAAALHLLRTALPGPGAERTRALALLLGLLSPLAVYQGWSAHPDGIETALVLSS
                     FAQTWRLAAEPDRAPLRRCALLAATIEAGALFKNYGLVLLPALPLAFAPRLLACWRAG
                     HRRLFAGALGAWLLVSASTALALCGLHPLQRIAGEGGGAEQYGRGDLLASAGGNLLCL
                     LVALLQGPGLATPFALACAPRRHVLWPLLAFAAIWVLGLLPFPHGYYNPRYLLPVLPI
                     VGLCAALGSQRLPRAFARAVAVSALAGAAALTVAFDVGAVHARIAPFVPALALRGPRM
                     PGLLDNLRLPLHEQARAWLGELDALPCGAVVYLLDFAYYGDAQHGVYERAGLIRGDLA
                     IRYATRRGFAPAAGDFYACFWTAPDAQVLEPFGEVAKVHDDLFLVRRR"
     gene            complement(1335..2405)
                     /locus_tag="VFA35_03930"
     CDS             complement(1335..2405)
                     /locus_tag="VFA35_03930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZT55352.1"
                     /translation="MSRPRPKWRSPRFLLKVLLANALVFAVVFALGEFAVRAWREGGL
                     VAGLQSFAWPNDRPHGAGTGDWFRADAELGYTLDPGRPEVSSLRIRHGELGPKEKGAF
                     RLLLIGDSVTWPADGFAALLAARLCAERPQVETINAAVPGYTAHQERLHLARLLAPVA
                     PDVVVVQYCCNDNHAFLHQLVGGGLLLTQAARTALLPPGDGPWARFQRWSYLAFELRR
                     RLAGPPAAAGPFPWRDDAAFEPAWRDDSWPMLLAEYDAMLALGNGAGARLLVLAMPHE
                     AQLADAALAADAAYTCMPQSRLGAWCTAHAVPFVDLHPAFVRHRDEHLFVRGDPVHLS
                     AAGHALVAAELHEALVAAHLLP"
     gene            complement(2418..2768)
                     /locus_tag="VFA35_03935"
     CDS             complement(2418..2768)
                     /locus_tag="VFA35_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55353.1"
                     /translation="MLRDLQRVLLAACLQKDPAAFLRDALARGEHGLSAGEQAMLRAA
                     SADGLRLCHVLVTKLRLERLLRGDAQAAAAARADPVAFMARFARYAAAVPPTAVFPSE
                     EAARFAAFAAQDRG"
     gene            complement(2762..>3076)
                     /locus_tag="VFA35_03940"
     CDS             complement(2762..>3076)
                     /locus_tag="VFA35_03940"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HZT55354.1"
                     /translation="YTQCLALGCALDAWLDRVPWPRVLEIHLSGGSDSDPDWLPSGRV
                     LRLDSHDGPVPGPVWAAFGRALPRAPNLRAVVLEWLPEGMTAAAAAQFAADFARARSM
                     LC"
BASE COUNT          355 a         1190 c         1148 g          383 t
ORIGIN      
        1 cttcgatccg cggcccgtgg tcgccgactc gcggctctcg tacgacaagc cgatcgcgtt
       61 cagctggctg gcgtcgccgt tcgtcgcggc gctcggcaac caccatggcc tgatggcggt
      121 gtcgctgctc ggcgcgctgg cgatgctggc cgccgcgctg cacctcctgc gcacggcgct
      181 gccggggccg ggcgccgaac gcacgcgcgc gctcgcgctg ttgctcggac tcctgtcgcc
      241 gctcgcggtc taccagggct ggtcggcgca ccccgacggc atcgagacgg cgctcgtgct
      301 gtcgtccttc gcgcagacct ggcggctcgc cgccgaaccg gaccgcgcgc cgctgcggcg
      361 ctgtgcgctg ctcgcggcga cgatcgaggc cggcgcgctg ttcaagaact acggcctcgt
      421 gctgctgccc gcgctgccgc tcgcgttcgc gccgcggctc ctcgcgtgct ggcgcgccgg
      481 ccaccgccgc ctgttcgccg gcgcgctcgg ggcgtggctg ctggtctccg cgtcgacggc
      541 gctggcgctg tgcggcctgc acccgctgca gcggatcgcc ggcgaaggcg gcggcgccga
      601 gcagtacggg cgcggcgacc tgctcgccag cgccggcggc aacctgctct gcctgctggt
      661 cgcgctgctg cagggaccgg gcctggcgac gccgttcgcg ctcgcctgcg cgccgcgccg
      721 gcacgtgctg tggccgctgc tcgcgttcgc cgcgatctgg gtgctcggcc tgctgccgtt
      781 cccgcacggc tactacaacc cgcgctacct gctgccggtg ctgccgatcg tcggcctgtg
      841 cgcggcgctc ggcagccagc gcctgccgcg cgcgttcgcg cgcgccgtcg cggtcagcgc
      901 gctcgcgggt gccgcggcgc tgacggtcgc cttcgacgtc ggcgccgtgc acgcgcgcat
      961 cgcgccgttc gtgccggccc tcgcgctgcg cggaccgcgc atgcccggcc tgctcgacaa
     1021 cctgcgcctg ccgctgcacg agcaggcgcg cgcatggctc ggcgagctcg acgcgctgcc
     1081 gtgcggcgcc gtcgtctacc tgctcgactt cgcctactac ggcgacgcgc agcacggcgt
     1141 ctacgaacgg gcggggctga tccgcggcga cctcgcgatc cgctacgcga cgcgccgcgg
     1201 cttcgcgccg gcggcgggcg acttctacgc ctgcttctgg acggcgcccg acgctcaggt
     1261 cctggagccg ttcggcgagg tcgcgaaggt gcacgacgac ctgttcctcg tgcggcgccg
     1321 ctgacggccg ccaatcacgg cagcagatgc gcggcaacga gcgcctcgtg cagctcggcg
     1381 gcgacgagcg cgtgcccggc ggcgctcagg tgcaccggat cgccgcgcac gaacagatgc
     1441 tcgtcgcggt ggcgcacgaa cgccggatgc aggtcgacga acggcacggc gtgcgcggtg
     1501 caccacgcgc cgagccgcga ctgcggcatg cacgtgtagg cggcgtcggc cgcgagcgcc
     1561 gcgtcggcga gctgcgcctc gtgcggcatc gcgagcacca gcagccgcgc gccggcgccg
     1621 ttgccgagcg ccagcatcgc gtcgtactcc gcgagcagca tgggccagct gtcgtcgcgc
     1681 cacgcgggtt cgaacgccgc gtcgtcgcgc cacgggaacg ggccggccgc cgccggcgga
     1741 ccggccaggc gtctgcgcag ctcgaacgcg aggtagctcc agcgctggaa gcgcgcccag
     1801 ggaccgtcgc cgggcggcag cagcgcggtg cgcgccgcct gcgtcagcag gagcccgccg
     1861 ccgacgagct ggtgcaggaa cgcgtggttg tcgttgcagc agtactgcac gacgacgacg
     1921 tcgggcgcga ccggcgccag caggcgcgcg aggtgcagcc gttcctggtg cgcggtgtag
     1981 cccgggaccg cggcgttgat cgtctcgacc tgcggccgct cggcgcagag ccgcgccgcg
     2041 agcagcgcgg cgaagccgtc ggcgggccag gtgaccgagt cgccgatcag cagcagccgg
     2101 aaggcgccct tctccttcgg gccgagctct ccgtggcgga tgcgcagcga gctgacctcg
     2161 ggccgccccg gatcgagcgt gtagccgagc tcggcgtcgg cgcggaacca gtcgccggtg
     2221 ccggcgccgt gcggccggtc gttcggccac gcgaacgact gcaggcccgc aacgaggccg
     2281 ccctcgcgcc acgcgcgcac tgcgaactcg ccgagcgcga acacgaccgc gaacaccagc
     2341 gcgttggcga gcaggacctt cagcaggaag cgcgggctgc gccatttcgg ccgcgggcgg
     2401 gacatcccgc gcgcatgcta cccgcggtcc tgcgccgcga acgccgcgaa ccgcgccgcc
     2461 tcctcgctcg ggaagaccgc ggtcggcggc accgccgcgg cgtagcgcgc gaagcgcgcc
     2521 atgaacgcga ccggatcggc gcgcgccgcg gccgcggcct gcgcgtcgcc gcgcagcagc
     2581 cgctcgaggc gcagcttcgt caccagcacg tggcagaggc gcaggccgtc ggcagaggct
     2641 gcgcgcagca tcgcctgctc gccggccgac aggccgtgtt cgccgcgcgc cagcgcgtcg
     2701 cgcaggaacg ccgcgggatc cttctgcaga cacgcggcca gcagcacgcg ctgcaggtcc
     2761 ctcagcacag catgctccgc gcgcgcgcga agtcggcggc gaactgcgcg gcggcggccg
     2821 cggtcatgcc ctcgggcagc cattcgagca ccaccgcgcg caggttcggc gcgcgcggca
     2881 gcgcgcgccc gaacgcggcc cacaccggcc cgggcaccgg cccgtcgtgc gaatcgagcc
     2941 gcagcacgcg gccgctcggc agccagtcgg gatcgctgtc gctgccgccg ctcaggtgga
     3001 tctcgagcac gcgcggccac ggcacgcggt cgagccacgc gtcgagcgcg cagccgagcg
     3061 cgagacactg cgtgta
//
LOCUS       DASRDG010000236         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_149809, whole genome shotgun sequence.
ACCESSION   DASRDG010000236 DASRDG010000000
VERSION     DASRDG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149809"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VFA35_03945"
     CDS             complement(<1..1289)
                     /locus_tag="VFA35_03945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZT55355.1"
                     /translation="MDHLSSADAAFLHFETPETPMHVGSLVVLDLPKGYDGDFYEDAK
                     AYLASRLHLLPAFRRKLVLMPFDLANPVWVEDRELDLDHHIRHVILPRPGSFAKLEQL
                     VGRLHAPLMDRSRPLWELYIIEGLASGQVALYSKLHHAGIDGQAGIALAKVLYSLSAS
                     PPPVKPPRIKPRARHDALGVAELAGAAIRNAGRQYASILKALPKTAKSVLDTLAPVSD
                     DDGRRRPGRLNRPKVAPKTLFNVSISNQRAFAARSVPFAEVREMAKRSACSVNDVVMA
                     VCAAALRRYLSEHRELPTEPLVAAIPVSLREQGNTDANNQVSMMLVGLATDVADPVER
                     LRTIHESAATAKKQIGNIRELPTDFPSFGAPWLLAGLAALAGRSKLAERLPSLINVLI
                     SNVPGPQVPLYFAGARIVANYPVSIPAHGTALNMTVQ"
     gene            complement(1312..>2894)
                     /locus_tag="VFA35_03950"
     CDS             complement(1312..>2894)
                     /locus_tag="VFA35_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZT55356.1"
                     /translation="PALEFLGRTINYRELGELADRAAKGLQQLGVGPGVHVGLFMPNT
                     PHYPIAFFGVLKAGGIVVNYSPLDAERVLEHKVEDSETDVLVTLDLAALYPQMARLLG
                     RSRLKRLVVGTLGEYSAAPDAVRGQLQAGKLLAEVGSDARHLSFARLLDNDGRFQPHP
                     IPDLTNAVAVLQYTGGTTGQPKGAMLTHGNLSAACAQYVETTKGNPPILDEGSERVLV
                     VLPLFHIYSLSVNMLLGMRLGALLVLQAKFDLDAAIKEIAAKKISVFAGVPTMYTAMI
                     NHPNAASLDLKSLKFCGSGGAPLPVELAQRFFALTGCRLNEGWGMTETSPTGTFTPAR
                     GKRKAGSCGLPIPGIRFKFEKVGSPGIDAVPGEPGELCIKGPNVMLGYWNNPQATAES
                     MTADGYFRTGDIARMDDEGFVFIVDRSKDMLLCGGFNVYPRVLEEAIQEHPAVAEVCV
                     IGIPDPYRGQSPKAFVALRPGAKPFGLDELKDFLKDRLGKHEMVQELEIRAELPKTPI
                     GKLSRKALVDEEARRRSS"
BASE COUNT          415 a          963 c         1037 g          479 t
ORIGIN      
        1 ctctgcaccg tcatgttgag cgcggtgccg tgcgccggga tcgacaccgg atagttggcg
       61 acgatgcgcg cgccggcaaa gtacagcggc acctgcggtc cgggcacgtt ggagatcagc
      121 acgttgatca gcgacggcag ccgctcggcc agcttcgagc gcccggcgag cgcggcgagc
      181 ccggcgagca accacggtgc gccgaacgac gggaagtcgg tcggcagctc gcggatgttg
      241 ccgatctgct tcttcgccgt cgcggccgat tcgtggatcg tgcgcagccg ctcgaccggg
      301 tcggcgacgt cggtggccag gccgacgagc atcatcgaga cttgattgtt tgcatccgtg
      361 ttgccctgct cgcggagcga caccggaatg gccgcgacca gcggctcggt cggcagctcg
      421 cggtgctcgc tcaggtagcg gcgcagcgcc gctgcgcaga ccgccatcac cacgtcgttg
      481 acgctgcagg cactgcgctt ggccatctcc ctgacctcgg cgaacggcac cgagcgcgcg
      541 gcgaaggcgc gctggttcga gatcgagacg ttgaacagcg tcttcggtgc gaccttcggg
      601 cggttgaggc ggcccgggcg acgccggccg tcgtcgtcgg acaccggggc gagcgtgtcg
      661 agcacgctct tcgcggtctt cggcaacgcc ttcaggatgc tcgcgtactg gcggccggcg
      721 tttcgaatcg ccgcccccgc aagctcggcg acgccgagcg cgtcgtgccg ggcgcgcggc
      781 ttgatccgcg gcggcttgac cggcggtggc gaggcgctga gcgagtacag caccttggcc
      841 agcgcgattc cggcctggcc gtcgattccg gcgtggtgca gcttcgagta cagcgccacc
      901 tgtccgcttg ccaggccctc gatgatgtac agctcccaca gcgggcggct gcggtccatc
      961 agcggggcgt gcagccggcc gacgagttgc tcgagcttcg cgaagctgcc cggccgcggc
     1021 aggatcacgt ggcggatgtg atggtcgagg tcgagctcgc gatcctcgac ccagaccggg
     1081 ttggccaggt cgaagggcat cagcacgagc ttgcgccgga atgccggcag caggtgcagg
     1141 cgcgacgcga ggtatgcctt ggcgtcttcg tagaaatcgc cgtcgtagcc cttgggcagg
     1201 tcgagcacga ccaggctgcc cacgtgcatc ggcgtttcgg gcgtctcgaa gtgcaggaag
     1261 gcagcatccg cgctgctcag gtggtccatt gcgggctccc gcttgcggtc ttcagctgct
     1321 tcgccggcgc gcttcctcgt ccacgagggc cttgcgcgac agcttgccga tcggcgtctt
     1381 gggcagctcg gcgcgtatct ccagctcctg caccatctcg tgcttgccga ggcggtcctt
     1441 caggaagtcc ttcagctcgt cgaggccgaa cggcttcgcg cccggcctga gcgcgacgaa
     1501 ggccttcggc gactggccgc ggtagggatc ggggatgccg atcacgcaga cctcggccac
     1561 cgccggatgc tcctggatcg cctcttcgag cacgcgcggg tagacgttga agccgccgca
     1621 cagcagcatg tccttgctgc ggtcgacgat gaagacgaag ccttcgtcgt ccatgcgcgc
     1681 gatgtcgccg gtgcggaagt acccgtccgc ggtcatcgac tcggccgtgg cctgagggtt
     1741 gttccagtag ccgagcatca cgttcggccc cttgatgcac agctcgcccg gctcgccggg
     1801 cacggcgtcg atgccggggc tgccgacctt ctcgaacttg aaccggatgc cggggatcgg
     1861 caggccgcac gagccggcct tgcgcttgcc gcgtgccggc gtgaaggtgc cggtgggcga
     1921 ggtctcggtc atgccccagc cctcgttcag ccggcagccg gtgagggcga agaagcgctg
     1981 ggccagctcc accggcagcg gtgcgccccc cgagccgcag aacttgagcg acttcaggtc
     2041 gagcgacgcc gcgttcgggt ggttgatcat cgccgtgtac atcgtcggca cgccggcgaa
     2101 cacgctgatc ttcttggccg cgatctcctt gatcgccgcg tcgaggtcga acttggcctg
     2161 cagtacgagc agcgcgccca gccgcatgcc gagcagcatg ttgacgctga gggaatagat
     2221 gtggaacagc ggcagcacga ccagcacgcg ctcggaaccc tcgtcgagga tcggcgggtt
     2281 tcccttcgtc gtttcgacgt actgcgcgca tgcggccgac aggttgccgt gggtcagcat
     2341 ggcgcccttg ggctgcccgg tggtgccacc ggtgtactgc aggacggcga ccgcgttcgt
     2401 gaggtcaggg atcgggtgcg gctggaagcg gccgtcgttg tcgagcaggc gggcgaagct
     2461 caggtgccgg gcgtcgctgc cgacctcggc gagcagcttg ccggcctgca actggccgcg
     2521 cactgcgtcg ggtgccgccg agtactcgcc gagcgtgccc acgaccagcc tcttcagccg
     2581 cgagcggccg agcaggcgcg ccatctgcgg gtacagcgcc gccaggtcga gggtgaccag
     2641 gacgtcggtc tcgctgtcct cgaccttgtg ctcgagcacg cgctcggcgt cgagcggcga
     2701 atagttgacg acgatgccgc cggccttcag cacgccgaag aacgcgatcg gatagtgcgg
     2761 tgtgttcggc atgaacagcc cgacgtgcac accggggccg acgccgagct gctgcaggcc
     2821 cttcgcggcg cggtcggcca actcgccgag ctcgcggtag ttgatcgtgc ggccgagaaa
     2881 ctcgagcgcc gggc
//
LOCUS       DASRDG010000237         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_840481, whole genome shotgun sequence.
ACCESSION   DASRDG010000237 DASRDG010000000
VERSION     DASRDG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840481"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..914
                     /gene="ugpE"
                     /locus_tag="VFA35_03955"
     CDS             63..914
                     /gene="ugpE"
                     /locus_tag="VFA35_03955"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HZT55357.1"
                     /translation="MVERRATLGILAHAIMILGILIVAFPLYLAFVASTHTSQEIVQA
                     PMPLLPGSHFWDNYSAALTGHGGGAGSNAPVGRMMWVSLVTALVITFGKIAVSLLSAF
                     ALVYFRFPLRGLFFWAIFVTLMLPVEVRILPTYKVVADLGLLNSYAGLTIPLIASATA
                     TFLFRQFFLTVPEELVEAARIDGAGPMRFFWDILLPLSKTSIAALFVIQFIYGWNQYL
                     WPLLATTSEDMYPVVVGIKRMIAGGDSGNEWNIVMATAIMAMLPPAIVVVLMQRWFVK
                     GLVDTEK"
     gene            934..2001
                     /locus_tag="VFA35_03960"
     CDS             934..2001
                     /locus_tag="VFA35_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HZT55358.1"
                     /translation="MASISLRNVVKRYGHGPKANPVIHGVSAEIADHEFIVIVGPSGC
                     GKSTLLRMVAGLEEISGGEIAIGGRVVNDTEPSERDIAMVFQNYALYPHMTVFDNMAY
                     GLKIAKVPLDEIKVRVDKAAKILELGPYLQRKPRELSGGQRQRVAMGRAIVRQPQVFL
                     FDEPLSNLDAKLRAQTRLEIQKLHADLGVTSLFVTHDQVEAMTLAQRMIVMNAGRMEQ
                     IGTPDEVYHRPATTFVANFIGSPPMNLIKGSADGSRFTAGGQTLALPASAPRSGELIL
                     GLRPEHAELSQTGSAGWQLKVEALEMLGAERLVYGHVGDTLFTVRLDATLAPPKVGDT
                     VALTTTPEHLHWFDAATTKRV"
BASE COUNT          343 a          694 c          654 g          342 t
ORIGIN      
        1 gatcgtcgtg tcgctcaccg tcctccaatt ccggtacgtc gaaaagaaag tgaactactg
       61 agatggtcga gcgccgcgcc acgctgggca tcctggccca cgccatcatg attctcggga
      121 tcctgatcgt cgcgttcccg ctgtaccttg ccttcgtcgc atccacccac acctcgcaag
      181 agatcgtgca ggcgccgatg ccgctgctgc ccggcagcca cttctgggac aactacagcg
      241 ccgccctcac cggccacggc ggcggcgccg gctcgaacgc gccggtcggc cggatgatgt
      301 gggtcagcct ggtcaccgcg ctggtcatca ccttcggcaa gatcgcggtc tcgctgctgt
      361 cggcgttcgc gctggtgtac ttccgcttcc cgctgcgcgg cctgttcttc tgggcgatct
      421 tcgtcacgct gatgctgccg gtcgaggtgc gcatcctgcc gacctacaag gtcgtcgccg
      481 acctcggcct gctcaacagc tatgccggcc tgacgatccc gctgatcgcc tcggcgaccg
      541 ccacattcct gttccgccag ttcttcctga cggtgcccga agaactggtc gaggccgcgc
      601 gcatcgacgg cgccggcccg atgcgcttct tctgggacat cctgctgccg ctgtcgaaga
      661 cctcgatcgc ggcgctgttc gtgatccagt tcatctacgg ctggaaccag tacctgtggc
      721 cactgctcgc gaccaccagc gaagacatgt acccggtcgt cgtcggcatc aagcgcatga
      781 tcgccggcgg cgattcgggc aacgagtgga acatcgtgat ggccaccgcg atcatggcga
      841 tgctgccgcc ggcgatcgtc gtggtcttga tgcagcgctg gttcgtcaag ggcctggtgg
      901 acaccgaaaa atagacaccg aaaagacaag cacatggcat ccatctccct caggaacgtc
      961 gtcaagcgct acggccacgg gccgaaggcg aacccggtga tccacggggt cagcgccgag
     1021 attgccgatc acgagttcat cgtcatcgtc gggccgtcgg gctgcggcaa atcgacgctg
     1081 ctgcgcatgg tcgccggcct cgaagagatt tcgggcggcg agatcgcgat cggcgggcgc
     1141 gtcgtcaacg acaccgagcc gagcgagcgc gacatcgcga tggtgttcca gaactacgcg
     1201 ctctacccgc acatgacggt gttcgacaac atggcctatg gcctgaagat cgccaaggtg
     1261 ccgctggacg agatcaaggt gcgcgtcgac aaggccgcga agatcctcga gctcggcccc
     1321 tacctgcagc gcaagccgcg cgagctctcc ggcgggcagc gccagcgcgt cgcgatgggg
     1381 cgcgcgatcg tgcgccagcc gcaggtcttc ctgttcgacg agccgctgtc caacctcgac
     1441 gcgaagctgc gcgcgcagac gcggctggag atccagaaac tgcatgccga cctgggcgtg
     1501 acctcgctgt tcgtcacgca cgaccaggtc gaggcgatga cgctggcgca gcgcatgatc
     1561 gtgatgaacg ccggtcgcat ggagcagatc ggcacgcccg acgaggtcta tcaccgcccg
     1621 gcgacgacct tcgtcgcgaa cttcatcggc tcgccaccga tgaacctgat caagggcagc
     1681 gccgacggtt cgcgcttcac cgcgggcggg cagacgctcg cgctcccggc ttcggcgccg
     1741 cgcagcggcg agctgattct cggcctgcgg cccgagcacg ccgaattgag ccagaccggc
     1801 tcggccggct ggcagctgaa ggtcgaggcg ctcgaaatgc tcggcgccga gcggctggtc
     1861 tacggccacg tcggcgacac gctgttcacg gtgcggctcg atgcgacgct ggcgccgccg
     1921 aaggtcggcg acacggtcgc gctgacgacc acgcccgagc acctgcactg gttcgacgcg
     1981 gcgacgacga agcgggtctg aaggatggaa agctggccct acccgctctg gat
//
LOCUS       DASRDG010000238         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_491398, whole genome shotgun sequence.
ACCESSION   DASRDG010000238 DASRDG010000000
VERSION     DASRDG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491398"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFA35_03965"
     CDS             complement(<1..381)
                     /locus_tag="VFA35_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55359.1"
                     /translation="MSRTAVCVYTLVVALLLLDVPRSAAPDGLDVAWQGLLQHDWNTG
                     ARFGVESVFTFGPAGFLFGGGVLFDREGMWAGFAAALIKALGTSVALTALAGRLPRPA
                     WRAGYLFCLPVLAMFGFGSDVFTCL"
     gene            complement(467..2086)
                     /locus_tag="VFA35_03970"
     CDS             complement(467..2086)
                     /locus_tag="VFA35_03970"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55360.1"
                     /translation="MTPPSARPRNAVQRVFDRLLVALFAAALAAPFVDRIVRSDDARG
                     PEPERRLPAPAPAFPRTLAALLAFPREFEKHWDDSFGLRDVLLRWHSYLKVSWFGVSP
                     DDRHVIGKDLWVYNQNSDVIDNWRGAIPFSGTQLEAWRHRLERRRDAARAAGAHYLFA
                     IAPDKPQIYPEYVPPRFAKIGPSRMDQLFEHMRAHSDVDVLDLRPALRAERANDRPDD
                     FVYYRLGTHWQMRGAIAGFNAFVDHLRPRFPGLRNLPLSAHTERAERTNRDSELYNMY
                     IGDFQFQTDHWWLLTGPDHHRRAENDEKLRRQVFVNDDQELPRVVMFHDSFGPYFENQ
                     WAEVCSRFVMSWSYDFDLGLVRRERPDCVIEFIVERALVFQDPTELTPGEADPESRFA
                     AARPLVALDVEHPALRQLFGGTFATGRDDTGPYVQLTTAGPLDSFWIDGVPPVEGQML
                     LARLDIETSEPGSVALHWRAGAEADWSPDRKVEAPVLAGRNTVHLVLPAQRLPLEGLV
                     LRPLDHPGNCRLRSLAVRAVDPAAGTVPASH"
     gene            complement(2091..>2429)
                     /locus_tag="VFA35_03975"
     CDS             complement(2091..>2429)
                     /locus_tag="VFA35_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55361.1"
                     /translation="VMIGWVFFRAENLAKAVDFLGNMVGIGSHAEPGRYSLSLWLDPV
                     LATSLAAAVIAATPVLPAALRRFGAAARPAFVVATAETAALLALAAMFVVSCTLMASG
                     SYNPFIYFRF"
BASE COUNT          400 a          830 c          890 g          309 t
ORIGIN      
        1 gaggcaggtg aacacgtcac tgccgaagcc gaacatggcg aggacgggca ggcagaacag
       61 gtagccggcg cgccacgcag ggcgcggcag ccggccggca agagccgtca gcgcgacgct
      121 cgttccgagc gccttgatca gcgccgccgc aaagcccgcc cacatgccct cgcgatcgaa
      181 caggacgccg ccgccgaaca ggaagccagc ggggccgaac gtgaacaccg actcgacacc
      241 gaacctcgcg ccggtgttcc agtcgtgctg cagcaacccc tgccacgcca catcgagccc
      301 gtcgggtgca gcggagcgcg ggacatccag caggagcagg gcaacgacga gcgtgtagac
      361 gcagacggcg gtgcgggaca tgaggggccg caatgatggg cgacactccg cactgccgcc
      421 agctgcccgc gcgtcgcgcg ccgcgaggac ttgcgctggc aggaggtcag tgactcgccg
      481 gcacggtgcc cgcggccggg tcgacggcgc ggaccgcgag cgagcgcaat cggcagttcc
      541 ccgggtgatc gagcggccgc agcaccagcc cttcgagcgg cagccgctgg gccggcaaca
      601 cgaggtggac cgtgttgcgg ccggcgagca cgggcgcctc gaccttgcgg tcgggcgacc
      661 agtcggcctc cgccccggcg cgccagtgga gcgcgacgct gcccggctcg gaggtctcga
      721 tgtccagccg cgccagcagc atctggccct cgaccggcgg cacgccatcg atccagaacg
      781 agtcgagcgg gccggcggtg gtcagctgca cgtacggacc ggtgtcgtcg cgtccggtcg
      841 cgaacgtgcc gccgaacaac tgccgcagcg ccgggtgctc gacgtcgagt gcgaccagcg
      901 gtcgcgcggc agcgaagcgg ctctcggggt cggcctcgcc gggcgtgagc tcggtcgggt
      961 cctggaacac cagcgcgcgc tcgacgatga actcgatcac gcagtcgggc cgttcgcggc
     1021 gcacgagccc gagatcgaag tcgtagctcc aggacatcac gaaccgcgag cagacctctg
     1081 cccactggtt ctcgaagtac ggtccgaacg agtcgtggaa catcacgact cgcggcagct
     1141 cctggtcgtc gttgacgaac acctgccggc gcagcttctc gtcgttctcg gcgcggcgat
     1201 ggtggtcggg accggtcagc aaccaccagt gatccgtctg gaactggaag tcgccgatgt
     1261 acatgttgta cagctcggag tcgcgattgg tcctctcggc gcgctcggtg tgcgccgaca
     1321 gcggcaggtt gcgcaacccg gggaaacgcg gccgcaggtg gtcgacgaac gcgttgaagc
     1381 cggcgatggc gccgcgcatc tgccagtgcg taccgagccg gtagtagacg aagtcgtcgg
     1441 ggcggtcgtt ggcgcgctcg gcccgcagcg ccgggcggag gtcgagcacg tcgacgtccg
     1501 aatgcgcgcg catgtgctcg aacagctggt ccatgcgcga aggcccgatc ttcgcgaacc
     1561 gcggcggcac gtactcgggg tagatctgcg gcttgtcggg cgcgatcgcg aacaggtagt
     1621 gggcgccggc cgcgcgcgcg gcgtcgcggc gccgctcgag gcgatgccgc cacgcctcga
     1681 gctgggtccc ggagaacggg atcgcgccgc gccaattgtc gatgacgtcg ctgttctggt
     1741 tgtagaccca gaggtccttg ccgatcacgt ggcggtcgtc gggcgacacg ccgaaccagg
     1801 acaccttcag gtagctgtgc cagcgcagca gcacgtcgcg cagcccgaag ctgtcgtccc
     1861 agtgcttctc gaactcgcgc ggaaacgcca gcagcgccgc cagcgtcctg ggaaacgccg
     1921 gcgcaggagc ggggagccgg cgttcgggtt ctggtccgcg cgcatcgtcg ctgcggacga
     1981 tgcggtcgac gaacggcgcc gccagcgccg cggcgaacag cgccacgagc aggcggtcga
     2041 agacgcgttg gacggcgttc ctggggcgcg cgctcggcgg ggtcatcggc tcagaaccgg
     2101 aagtagatga acgggttgta ggaccctgac gccatcagcg tgcagctgac gacgaacatc
     2161 gccgcgagcg ccagcagcgc cgcggtctcg gcggtggcga cgacgaacgc cggccgcgcc
     2221 gcggcgccga atcggcgcag cgccgccggc agcacgggcg tcgcggcgat cacggcggcc
     2281 gcgagcgacg tcgccagcac cgggtcgagc cacagcgaca ggctgtagcg ccccggttcg
     2341 gcgtggctgc cgatgccgac catgttgccg aggaagtcga cggccttcgc caggttctcc
     2401 gcgcggaaga acacccagcc gatcatcac
//
LOCUS       DASRDG010000239         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_150164, whole genome shotgun sequence.
ACCESSION   DASRDG010000239 DASRDG010000000
VERSION     DASRDG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150164"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFA35_03980"
     CDS             complement(<1..322)
                     /locus_tag="VFA35_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZT55362.1"
                     /translation="MADSRRVVFYHSPQSRSAGALILLEELGADYELRPLNLQKGESR
                     ASEYLGVNPMGKVPAIVHNGALVTEQGAVYTYLADLYPEVGITPAIGDPLRGPYLRWM
                     FFYNA"
     gene            complement(362..1129)
                     /locus_tag="VFA35_03985"
     CDS             complement(362..1129)
                     /locus_tag="VFA35_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZT55363.1"
                     /translation="MLELLTDPQAWIALATLTALELVLGIDNIVFISILVDKLPVAQR
                     ERARRIGLFMAMFMRIGLLLVLSWIVGLVEPLFSVFGKGISGRDLILIAGGLFLIYKA
                     TGEIHQSLEGEEGHASSAVEATFTAVILQIMVIDIVFSLDSIITAVGMVDRLAVMIAA
                     VVASVGLMMLFAGAIGRFVSDHPTIKVLALSFLVVVGVVLIAEGFGQHVPKGYIYFAM
                     AFAVGVEMLNITMRGRAKRAAARREEATAYREESAPR"
     gene            complement(1179..2222)
                     /locus_tag="VFA35_03990"
     CDS             complement(1179..2222)
                     /locus_tag="VFA35_03990"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZT55364.1"
                     /translation="MTIAFDTIPRERVPELLRTMPKAELHIHIEGSLEPELIFALAQR
                     NGVSLPYANVEALRAAYAFTDLQSFLDIYYAGASVLLHEQDFFDMGMAYFRRAAADHV
                     VHAELFFDPQTHTERGVAIGTVIDGLTRACETAQRELGVSGALILCFLRHLSEEAAFA
                     TLAAALPYRERFIGVGLDSAEKGNPPEKFAHVFARCRELGLHLVAHAGEEGPPAYIRS
                     ALDVLHVERIDHGVACLGDPALVERLAAERIPLTVCPLSNVKLCVYPTMREHDLAALL
                     AAGLCVSVNSDDPAYFGGYINQNFIEAFAALPQLTAKHAHQLAANSFEASFVDAATKA
                     RWSRALDKVFAAG"
BASE COUNT          391 a          751 c          817 g          358 t
ORIGIN      
        1 aggcgttgta gaagaacatc cagcgcaggt acgggccgcg cagcgggtcg ccgatcgccg
       61 gcgtgatgcc gacctccggg tagagatcgg ccaggtaggt atagacggcg ccctgctcgg
      121 tgacgagagc gccgttgtgg acgatcgccg gcaccttgcc catcggattg acgccgaggt
      181 actcgctcgc tcgcgactcg cctttttgca ggttgagcgg gcgcagctcg tagtcggcgc
      241 cgagctcctc gagcaggatc agcgcgccgg cggagcgcga ctgcggcgag tgatagaaga
      301 cgaccctgcg actgtcggcc atcgcgatct cccgaagttg ggatcggtgt tgcgcgcgcc
      361 ctcagcgcgg cgcggactct tccctgtagg ccgtcgcttc ctcccgccgg gctgcggccc
      421 gcttggcacg accgcgcatg gtgatgttga gcatctcgac gccgacggcg aacgccatcg
      481 cgaagtagat gtagcccttg ggcacgtgct ggccgaagcc ctcggcgatc agcacgacgc
      541 cgacgacgac gagaaaggac agcgccagca ccttgatcgt cgggtggtcg gagacgaacc
      601 gcccgatcgc gccggcgaac agcatcatca gcccgaccga ggcgaccacc gcggcgatca
      661 tcaccgcgag gcggtcgacc atgccgaccg cggtgatgat cgagtcgagc gagaacacga
      721 tgtcgatcac catgatctgc aggatcaccg cggtgaaggt ggcctcgacc gcgctcgacg
      781 catgcccctc ctcgccttcg agcgactggt ggatctcgcc ggtcgccttg tagatcagga
      841 acaggccgcc ggcgatcagg atcaggtcgc ggcccgagat gcccttgccg aacacgctga
      901 acagcggctc gaccagcccg acgatccacg acagcaccag caacaggccg atgcgcatga
      961 acatcgccat gaacaggccg atgcgccgcg cgcgctcgcg ctgcgcgacc ggcagcttgt
     1021 cgaccaggat cgagatgaag acgatgttgt cgatgcccag caccagctcg agcgcggtca
     1081 gcgtggccag tgcgatccag gcctgtggat cggtcagaag ttcgagcatc ggagtcgtcg
     1141 ccttgggttg agacggcgcc gatgataggt gggcaaactc agccggcggc aaagaccttg
     1201 tcgagcgccc ggctccagcg cgccttggtg gcggcgtcga cgaagctggc ctcgaagctg
     1261 ttggccgcca gctggtgcgc gtgcttggcc gtcagctgcg gcagcgccgc gaaggcctcg
     1321 atgaagttct ggttgatgta gccgccgaag tacgccgggt catcggagtt gacgctcacg
     1381 cacaggccgg cggcgagcag cgcggccaga tcgtgctcgc gcatcgtcgg gtagacgcag
     1441 agcttgacgt tcgacaacgg gcacaccgtc agcggaatgc gttcggcggc gaggcgctcg
     1501 accagcgccg gatcgcccag gcacgcgacg ccgtggtcga tgcgctcgac atgcagcacg
     1561 tcgagcgcgc tgcggatgta ggccggcggt ccctcctcgc cggcgtgtgc gacgaggtgc
     1621 aggccgagct cgcggcagcg cgcgaacacg tgtgcgaact tctcgggcgg gttgcccttc
     1681 tctgcactgt cgaggccgac gccgatgaag cgctcgcggt acggcagcgc ggcggccagc
     1741 gtcgcgaacg cggcctcttc gctgaggtgg cgcaagaagc acaggatcag cgcgccgctg
     1801 acgccgagct cgcgctgcgc ggtctcgcag gcccgcgtga ggccgtcgat cacggtgccg
     1861 atggccacac cgcgctcggt gtgggtctgc gggtcgaaga acagctcggc gtgcacgacg
     1921 tggtcggccg cggcgcgccg gaagtacgcc atgcccatgt cgaagaagtc ttgctcgtgc
     1981 agcagcacgc tggcgccggc gtagtagatg tcgagaaagc tttgcaggtc ggtgaacgcg
     2041 taggccgcgc gcagcgcctc gacgttcgcg tacggcaggc tgacgccgtt gcgctgcgcg
     2101 agcgcgaaga tcagctcggg ttcgagcgag ccttcgatgt ggatgtgcag ctcggccttg
     2161 ggcatcgtgc gcaacagctc gggcacgcgc tcgcgcggga tcgtgtcgaa ggcgatggtc
     2221 attgttcacc cctcaaagag aaggggtcgc cagaggcgac cccgctgtcc atgtcggcgt
     2281 agccgctccc gaagcctact tcttatcgcc gcccggg
//
LOCUS       DASRDG010000240         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_607657, whole genome shotgun sequence.
ACCESSION   DASRDG010000240 DASRDG010000000
VERSION     DASRDG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607657"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..2327)
                     /gene="rpoD"
                     /locus_tag="VFA35_03995"
     CDS             complement(129..2327)
                     /gene="rpoD"
                     /locus_tag="VFA35_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZT55365.1"
                     /translation="MTAKKSAPAPAPKAAPASKTAAKAKAEDTSKKAVKPAAKAAEVV
                     AKGKPGRKAKVDDKAKKSEDNDVDLSDLESDLEGEPEAEAAADETKVKAKPLRMKVSR
                     AKERALMREFGLEETALTEEEATKRRQELKTLIKMGKTRGFLTHQEINDHLPEKLVDA
                     EILEAIVSMLNDMGIAVYEQAPDAATLLIAGGGTTTATEEEAEEAAEAALSTVDSEFG
                     RTTDPVRMYMREMGTVELLTREGEIEIAKRIEGGLQSMMFAISASPTTIAEILTYADK
                     IRAGDMKISEAVDGFVTEDEADDYVAEEDVDFFDEEDDDDGQGGSKALTKKLEELKTA
                     AIQKFDALSVNFDKMRKAFEKDGYQSPSYNKAQQAISNELMTIRFTVKTIEKLCGILR
                     SQVDDVRRYERELRKILVDKCGMPQEYFIKHFPPNILNLKWAEKEVAANKPYSVILAR
                     NLPPVQELQKKLTDQQAKAVVPLDDLKRINKRMNEGEKASRDAKKEMIEANLRLVISI
                     AKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQAR
                     TIRIPVHMIETINKMNRLSRQHLQEFGFEPDAPTLAEKMEIPEDKIRKIMKIAKEPIS
                     METPIGDDDDSHLGDFIEDTNNTAPIEAAMQAGLRDVVKDILDSLTPREAKVLRMRFG
                     IEMSTDHTLEEVGKQFDVTRERIRQIEAKAIRKLKHPSRSDKLRTYLDTL"
     gene            complement(2642..4465)
                     /gene="dnaG"
                     /locus_tag="VFA35_04000"
     CDS             complement(2642..4465)
                     /gene="dnaG"
                     /locus_tag="VFA35_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZT55366.1"
                     /translation="MIPPTFIQDLLSRVDIVEVVGRHVQLKKGGANLSGLCPFHAEKS
                     PSFTVSPSKQFYHCFGCGANGDAIRFLTEYSGMSFIDAVKDLAQQVGLQLPEEDSSPA
                     ERAHAAAQKQRRSTLTEVLAKAADAYRKHLKQSPRAIDYLKGRGLSGEIAQQFGLGYA
                     PDGWRSLASVFPSYDDPLLEESGMVIAKDGEDGAADEADRKRYDRFRDRIMFPIRSVQ
                     GDVIGFGGRVLDRGEPKYLNSPETPVFSKGRELYGLFEGRTAIRQRGYALVVEGYMDV
                     VALAQSGFGNAVATLGTACTPDHLQKLFRFTESVVFSFDGDKAGRRAAGRALEASLPH
                     ARDTRTIRFLFLPAEHDPDSYVREFGPAAFEACVAQAVPLSRQLIEAAGVGCELATAE
                     GRAHMLANAKPLWSALPDGTLKRQLLADIAAAARDDADQLLHQWGHAPARVAPREGGT
                     SAYRAPARRTGRVSKGAANLLDRAIWLLLHRTELWAQLDGEAHDLLAAQPAPYDAFFG
                     CIERCVHEHGPLAPAAMLAELRHPSAADGEGAAVLSRIAAFHDPEPASDIAHELSLVI
                     VKLRLNAVDEELKQLFESGTASPDTHKRAGELMALRKRLKA"
BASE COUNT          609 a         1513 c         1558 g          942 t
ORIGIN      
        1 gcccacgcgg gtcagacgtg ggcttgtgcc ggtcgatgtc tcgtggttgc gacgccgacg
       61 cctgccgcgc gaattgcgag accgcttcgc ggccaacccg ctgtcgcggg tcacccatca
      121 ggtcaccctt acagggtgtc cagataggtg cgcaatttgt ccgagcggct cgggtgcttg
      181 agcttgcgga tcgccttcgc ctcgatctgg cggatgcgct cgcgggtcac gtcgaactgc
      241 ttgcccactt cctcgagcgt gtggtcggtg ctcatctcga tgccgaagcg catgcgcagc
      301 accttggctt cgcgcggcgt cagcgagtcg aggatgtcct tcacgacatc gcgcaggccg
      361 gcctgcatcg ccgcctcgat cggtgcggtg ttgttggtgt cctcgatgaa gtcgcccagg
      421 tgcgaatcgt cgtcgtcgcc gatcggcgtt tccatcgaga tcggctcctt cgcgatcttc
      481 atgatcttgc ggatcttgtc ttccgggatc tccatcttct cggccagcgt cggcgcatcg
      541 ggctcgaagc cgaactcctg caggtgctgg cgcgacaggc ggttcatctt gttgatcgtc
      601 tcgatcatgt gcaccggaat gcggatcgtt cgcgcctgat cggcgatcga gcgcgtgatc
      661 gcctggcgga tccaccaggt cgcgtaggtc gagaacttgt agccgcggcg gtattcgaac
      721 ttgtcgaccg ccttcatcag gccgatgttg ccctcttgaa tcaggtcgag gaactgcagg
      781 ccgcggttgg tgtacttctt cgcgatcgag atcaccaggc gcagattcgc ctcgatcatc
      841 tccttcttcg cgtcgcggct ggccttctcg ccttcgttca tgcgcttgtt gatgcgcttg
      901 aggtcgtcga gcggcaccac ggccttggcc tgctggtcgg tcagcttctt ctgcagttcc
      961 tgcaccggcg gcaggttgcg cgccaggatc acgctgtacg gcttgttggc cgcgacttcc
     1021 ttctcggccc acttcaggtt caggatgttc ggcgggaagt gcttgatgaa gtattcctgc
     1081 ggcatgccgc atttgtcgac caggatcttg cgcagctcgc gctcgtagcg gcgcacgtcg
     1141 tcgacctgcg atctcaggat gccgcacagc ttctcgatcg tcttgaccgt gaagcggatc
     1201 gtcatcagct cgttgctgat cgcctgctgc gccttgttgt acgagggcga ctggtagccg
     1261 tccttctcga aggccttgcg catcttgtcg aagttgacgc tgagcgcatc gaacttctga
     1321 atcgcggccg tcttcaactc ttcgagcttc ttcgtcagtg ccttgctgcc gccctggcca
     1381 tcgtcgtcgt cttcctcgtc gaagaagtcg acgtcttctt cggccacgta gtcgtcggcc
     1441 tcgtcttcgg tgacgaagcc gtcgaccgcc tcggagatct tcatgtcgcc ggcgcgaatc
     1501 ttgtcggcgt aggtgaggat ctcggcgatc gtcgtcggcg acgcgctgat cgcgaacatc
     1561 atcgactgca ggccaccttc gatgcgcttg gcgatctcga tctcgccttc acgcgtcagc
     1621 agttcgaccg tgcccatctc gcgcatgtac atgcgcaccg ggtcggtggt gcggccgaac
     1681 tcggagtcga cggtcgacag cgctgcttcg gctgcctctt cggcttcttc ttcggtcgcg
     1741 gtggtcgtgc cgccgccggc gatcagcaac gtcgcggcgt cgggtgcctg ctcgtagacc
     1801 gcgatgccca tgtcgttgag catcgagacg atggcctcga ggatctcggc gtcgaccagc
     1861 ttctcgggca ggtggtcgtt gatttcctgg tgcgtcagga agccgcgcgt cttgcccatc
     1921 ttgatgagcg tcttgagctc ctggcgccgc ttggttgcct cttcttcggt cagcgcggtc
     1981 tcttcgaggc cgaactcgcg catcagcgcg cgctccttgg cgcgcgaaac cttcatccgc
     2041 agcggcttgg ccttgacctt ggtctcgtcg gcggctgctt cggcttcggg ctcgccttcc
     2101 aggtcggact cgagatccga caggtcgacg tcgttgtctt ccgacttctt cgccttgtcg
     2161 tcgaccttcg ccttgcggcc cggcttgccc ttggcgacca cctctgccgc cttggccgcg
     2221 ggcttcacgg ccttcttgct cgtgtcctcg gccttcgcct tggcggcggt cttgctggca
     2281 ggcgcggctt tcggggccgg tgcgggggcg ctcttcttcg cggtcatgcg ggaatccttg
     2341 gatgatggtg gccagcgcgg tggcggcccg gccattgagt tgcgcacaaa gtggcgcaat
     2401 tcaagtcaag ccaacgcagc gtttgatcgg cgcggggcca atcggttgca gcgggcaagc
     2461 tcgggcgaac atttggggtg cagcccttgc gggaaaaggt ggcctgaaac ccgcgattgc
     2521 ggtgcctctt tcggagggtg gccgggcccg caggagatgc tgctgtcgcg cccttgtcga
     2581 tcttgccgcg ctggcgtaat cgaggattat accgtcggca aggtcggaat gccaatgacg
     2641 atcaggcttt caggcgcttg cgcaaggcca tcagctcgcc cgcgcgcttg tgcgtgtcgg
     2701 gcgacgccgt gcccgactcg aacagctgct tcagctcttc gtcgaccgcg ttcaggcgca
     2761 gtttgacgat caccagcgac agctcgtgcg cgatgtcgga cgccggctcc gggtcgtgga
     2821 acgcggcgat ccgcgacagc accgcagcgc cttcgccatc ggccgcggac gggtggcgca
     2881 gctcggcaag catcgccgcc ggcgcaagcg ggccgtgctc gtgcacgcag cgctcgatgc
     2941 agccgaagaa cgcgtcgtag ggcgccggct gcgcggcgag caggtcgtgt gcctcaccgt
     3001 cgagctgcgc ccagagttcg gtgcggtgca gcagcagcca gatcgcgcgg tcgagcaggt
     3061 tcgccgcgcc cttgctgacg cgcccggtgc ggcgcgcggg ggcgcggtag gcgctcgtgc
     3121 caccctcgcg cggcgcgacc cgcgccggcg cgtggcccca ctggtgcagc agctggtcgg
     3181 cgtcgtcgcg cgcggcggcg gcgatgtcgg cgagcaactg gcgtttcagg gtgccgtcgg
     3241 gcagggcgct ccacagcggc tttgcgttgg ccagcatgtg ggcgcggccc tcggcggtcg
     3301 cgagttcgca gccaacgcct gcggcctcga tcagctggcg cgacagcgga acggcctgcg
     3361 cgacacacgc ctcgaacgcg gccggcccga actcgcgcac ataggaatcg gggtcgtgct
     3421 cggccggcag gaacaggaag cggatcgtgc gcgtgtccct cgcatgcggc aggctggcct
     3481 cgagcgcgcg cccggcggcg cggcgcccgg ccttgtcgcc gtcgaagctg aacacgaccg
     3541 attcggtgaa gcgaaacagc ttctgaaggt ggtcgggcgt gcaggcggtg ccgagcgtcg
     3601 cgaccgcatt gccgaagccc gattgcgcga gcgcgaccac gtccatgtag ccttcgacga
     3661 cgagcgcgta gccgcgctgg cggattgccg tgcggccctc gaacaggccg tagagttcgc
     3721 ggcccttgct gaacaccggc gtctcgggcg aattcaggta cttgggctcg ccgcggtcga
     3781 gcacgcggcc gccgaagccg atcacatcgc cctgcacgga gcggatcgga aacatgatgc
     3841 ggtcacgaaa ccggtcatac cgttttcggt cggcttcgtc ggcggcgcca tcctcgccgt
     3901 ccttcgcgat caccatgccg ctctcttcga gcagcgggtc gtcgtagctc gggaacacgc
     3961 tcgcgaggct gcgccagccg tcgggcgcgt agcccaggcc gaactgctgc gcgatctcgc
     4021 ccgagaggcc gcggcctttc aggtagtcga tcgcgcgcgg gctttgcttc aggtgcttgc
     4081 ggtacgcgtc ggcggctttc gcgagcacct cggtcagggt cgaacggcgc tgcttctgtg
     4141 cggcggcgtg cgcgcgctcg gcgggcgagc tgtcttcctc cggcagttgc aggccgactt
     4201 gttgcgccag gtctttcacc gcgtcgatga agctcatgcc gctgtactcg gtgagaaagc
     4261 ggatcgcgtc gccgttcgca ccgcagccga agcagtgata gaactgcttg ctcgggctga
     4321 ccgtgaagct cggcgacttc tcggcgtgga acgggcacag cccgctcagg ttcgcgccgc
     4381 ccttcttcag ctgcacgtgg cggccgacga cctcgacgat gtcgacgcgc gacagcaggt
     4441 cctggatgaa ggtcggcggg atcaccggtc gagtctaact tcgcgccttt cgggcgctgc
     4501 gccgtcgcgc cggtccgggt gttcggcctc agaccggcgt cagcacctcg gcccggtgcg
     4561 gcatgccctt gtaggcgcgc acccagtcgg cgacctgttc ggccggcatc gccgcggcga
     4621 tg
//
LOCUS       DASRDG010000241         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_396973, whole genome shotgun sequence.
ACCESSION   DASRDG010000241 DASRDG010000000
VERSION     DASRDG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396973"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VFA35_04005"
     CDS             <1..1476
                     /locus_tag="VFA35_04005"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HZT55367.1"
                     /translation="DLIHGKDFADRIKRLMAMFNRYAGRELYIDNAIFLSERTTGHRN
                     RAIGHMLRNFEILDDPDPALDLYFRQCSVAVDCRDLSLIAATLANAGVHPVTGEQALD
                     PAHVERVLSVMTTCGLYDAAGEWVFRVGMPAKSGVGGGIMVVLPGQFGIGVFSPRLDA
                     HGNSVRGLQVCRDLSRELDLHVLRAARSSRSVFRARYCIADVNSKRRRPEAERAAIRR
                     AGSAVRVYELQGDLLFPAIEAVVSAVVDRAADLATAVLDLKRVSAVGGPAARILFDLI
                     ESMHTRGQQLVLASCERHGRLLRSLGEQRAAAGAEWRLVCLPDLDSAIEWCENGLLMG
                     AGAGKGGGQVPVVPLADNDLCRHLAPAAIAVLEPLLATRSYAPGAYIVSHGEPADALY
                     LITRGQVTVALPSVATGPKRLSTLSAGLSFGELAMINGGTRSADVIAVTAVECRVLDA
                     PALARLGASHPEIEIALLKNMLRSAHEIVNRLSQEVTALAG"
     gene            1526..>2527
                     /locus_tag="VFA35_04010"
     CDS             1526..>2527
                     /locus_tag="VFA35_04010"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZT55368.1"
                     /translation="MLRDLLNAYAVPDSRFDEMLASAGVPRPHWDAFLRALVAREGGE
                     IGDTLSLMEREIRENGITYNVYADPKGADRPWEVDPLPLLLSASEWETIESGIAQRAD
                     LLNRVLADIYGPQALLRSGAIPAPVVFGHSGFLHPLKGVRPAGGVHLFQYAADLARAP
                     DGSWWVVSDRTQAPSGAGYALENRLVVSRVFPQMFSDLNVQHLASFFAGVHDSLLHWA
                     PKPAPSDDTRAGAPLIVLLTPGPYNETYFEHALLARYLGFPLVEGSDLTVRNGCVWMK
                     TVEGLKRVHAILRRQDDDYCDPLELRSDSALGVAGLTDCVRRGNVFVGNALGSGVIES
                     "
BASE COUNT          350 a          907 c          889 g          381 t
ORIGIN      
        1 gacctcatcc acggcaagga cttcgccgac cgcatcaagc gcctcatggc gatgttcaac
       61 cgctacgccg gccgcgagct ctacatcgac aacgcgatct tcctctccga gcgcaccacc
      121 ggccaccgca accgcgcgat cggccacatg ctgcgcaact tcgagatcct cgacgacccc
      181 gatccggcgc tcgacctgta ctttcgccag tgctcggtgg cggtcgactg ccgcgacctg
      241 agcctgatcg cggccacgtt ggccaacgcc ggcgtgcacc cggtgacggg cgagcaggct
      301 ctcgaccccg cgcacgtcga gcgcgtgctc agcgtgatga ctacctgcgg cctctacgac
      361 gcggccggcg agtgggtgtt tcgcgtcggc atgccggcta agagcggcgt cggcggcggg
      421 atcatggtcg tgttgccggg ccagttcggc attggcgtgt tctcgccgcg gctcgatgcg
      481 catggcaaca gcgtgcgcgg cctgcaggtc tgccgcgacc tctcgcgcga gctcgacctg
      541 cacgtgctgc gcgcggcgcg ttcgtcacgc tcggtgttcc gcgcgcgcta ctgcattgcc
      601 gatgtgaact cgaagcgccg ccgccccgag gccgagcgcg cggcgatccg ccgcgccggg
      661 agcgcggtgc gggtctacga gttgcagggc gacctgctgt tcccggcgat cgaggcggtg
      721 gtcagcgcgg tggtcgatcg cgcggccgac ctcgcgactg cggtcctcga cctcaagcgc
      781 gtcagcgcgg tcggcggccc ggccgcgcgc atcctgttcg acctgatcga atcgatgcac
      841 acgcgcgggc agcaactcgt gctcgcctcc tgcgagcgcc acgggcgcct gctgcgctcg
      901 ctcggcgagc aacgcgccgc ggccggtgcc gaatggcgcc tcgtgtgcct gcccgacctc
      961 gacagcgcga tcgaatggtg cgagaacggg ctgctgatgg gtgcgggcgc gggaaagggc
     1021 ggagggcagg tgccggtcgt gccgctcgcc gacaacgatc tgtgccggca cctcgccccg
     1081 gccgcgatcg cggtcctcga gccactgctc gcgacgcgca gctacgcgcc cggcgcgtac
     1141 atcgtgagcc acggcgagcc ggcggatgcg ctctacctga tcacgcgcgg gcaggtcacg
     1201 gtggcgctgc cgtcggtcgc gaccgggccg aagcggctgt cgacgctgtc cgcgggcctg
     1261 agcttcggcg aactcgcgat gatcaacggc ggcacccgct cggccgacgt gatcgcggtc
     1321 accgcggtcg aatgccgggt gctggacgcg ccggcactgg cgcgcctggg cgcgagccat
     1381 cccgagatcg agatcgcttt gctgaagaac atgttgcgca gcgctcacga gatcgtgaac
     1441 cgtctgagcc aggaagtcac cgcgctcgcg ggctgagcaa cggtgctgcg gcgacaatgc
     1501 gcgctctgac gatgccgacc gcccgatgct gagagacctg ctcaacgcct acgccgtgcc
     1561 cgacagccgc ttcgacgaga tgctggcgtc ggcaggcgtg ccccgcccgc actgggacgc
     1621 gttcctgcgc gcgctcgtcg cgcgcgaggg tggcgagatc ggcgacacgc tgtcgctgat
     1681 ggagcgcgag atccgcgaga acgggatcac ctacaacgtc tatgccgacc caaagggcgc
     1741 cgaccggccg tgggaggtcg acccgctgcc gctgctgctg tcggcgagcg aatgggagac
     1801 gatcgaatcc ggcatcgcgc aacgcgccga cctgctgaac cgcgtgctgg ccgacatcta
     1861 cggcccgcag gcgctgctgc gcagcggcgc gatccccgcg ccggtggtct tcggccacag
     1921 cggcttcctg cacccgctga agggtgtgcg gccggccggc ggcgtgcacc tgttccagta
     1981 cgcggccgat ctggcgcgcg cgcccgacgg cagctggtgg gtcgtcagcg atcgcacgca
     2041 ggcgccttcg ggcgcgggct acgcgctcga gaaccggctc gtcgtctcgc gcgtgttccc
     2101 gcagatgttc agcgacctga acgtgcagca cctcgcgtcg ttcttcgccg gcgtgcacga
     2161 ctcgctgctg cactgggcgc ccaagcccgc ccccagcgac gacacccgcg ccggcgcgcc
     2221 gctgatcgtg ctgctgacgc cgggcccgta caacgagacc tacttcgagc acgcgctgct
     2281 ggcgcgctac ctcggctttc cgctcgtcga gggcagcgac ctgacggtgc gcaacggctg
     2341 cgtctggatg aagacggtcg agggcctgaa gcgcgtgcac gcgatcctgc gccgccagga
     2401 cgacgactac tgcgacccgc tcgagctgcg ttcagactcg gcgctcggcg tcgccggcct
     2461 gaccgactgc gtgcgccgcg gcaatgtgtt cgtcggcaac gcgctcggct cgggcgtgat
     2521 cgagtcg
//
LOCUS       DASRDG010000242         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1642307, whole genome shotgun sequence.
ACCESSION   DASRDG010000242 DASRDG010000000
VERSION     DASRDG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642307"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFA35_04015"
     CDS             <1..734
                     /locus_tag="VFA35_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZT55369.1"
                     /translation="RARPDGYTLMIGYVATHATNPAVKRRPGYDAIKDFTPIAMIGGT
                     PNLLVVARNVPVATLKEFIDYAKANPGKVDYGTSGVGTLNHLVMEQFKHASGVPSMAV
                     PYRSIGQAFVDAAGGQIQVIFPGLAAAMPHVRSGAFRPLAVTGARRHPLLPAVPTFKE
                     SGFDGFEGVTWYGIVGPAGLPDAIVRRLHEEVVKALAAPDLRETFAAQAIDVMPMTTA
                     QFGQYIADEIAHWTAVARASKIEGD"
     gene            753..2120
                     /locus_tag="VFA35_04020"
     CDS             753..2120
                     /locus_tag="VFA35_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZT55370.1"
                     /translation="MTLPSISVSQRLAAAFAGQTAALPPAVAQLCDAVLMDVTGLCVA
                     ARHSDYVRATLQATAGPGVCTAIGHAGSFNVATAALCNGTAAHGEDYDDTFVGGPVHA
                     GAVIVPAVLAAAEAHGLCGADVERGIAVGCELMCRLCRVAPKRVHQAGFHPTAVFGAL
                     GAAAGVASAMRLDLTAWCHALGIAGSLASGIIEYLAEGAWTKRMHPGWAAQAGYRAAR
                     LAQAGFTGPRTLFDGEHGFFHAFANSDGCDFSSMLDGAGHEWLCSDIAFKPYACGTMA
                     HPYIDCARRLVGEGLDPNDVASIECKTAEGIVHRLWEPLEAKQNPANGYAAKFSIPYA
                     IAVGMLRGDAGLGDYGEAVVHDPAVRRLAGRVRYVIDPDNPYPRQFTGHLRVALHSGE
                     VREASQGHFRGGRDEPMSPATLESKFLANCAYGGWDGQRARRALAVLRGLRAAPRVAL
                     DALRA"
     gene            2140..>2729
                     /locus_tag="VFA35_04025"
     CDS             2140..>2729
                     /locus_tag="VFA35_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZT55371.1"
                     /translation="MDAAPLKGRVALVTGAGRNIGRAIALALADAGAQVAVNVRASRL
                     EGQGVVDEILARGGDALLALADVTQRAQVDAMVAAITQRFGRLDIVVNNAAIRLEAPF
                     DELRYADWQAAHAVCVDGAFHCTQSALPHLRRSDAGSVINIGGMTAHTGAIHRAHVVS
                     AKAALGGLTRALAHELADSAITVNCVSPGLIDTVRQP"
BASE COUNT          396 a          964 c          975 g          394 t
ORIGIN      
        1 cacgtgcccg ccccgacggc tacacgctga tgatcggcta cgtcgcgaca cacgccacca
       61 acccggccgt gaagcgccgg cccggctacg acgcgatcaa ggacttcacg ccgatcgcga
      121 tgatcggcgg cacgcccaat cttctggtgg tcgcgcgcaa cgtgccggtc gccacgctga
      181 aggagttcat cgactacgcc aaggccaacc ccggcaaggt ggactacggc acctcaggcg
      241 tcggcacgct gaaccatctg gtgatggagc agttcaagca cgcctcgggc gtgccgagca
      301 tggcggttcc ctaccgcagc atcggccagg cgttcgtcga tgcggccggc ggccagatcc
      361 aggtgatctt ccccggcctc gccgcggcga tgccgcacgt gcgctcgggc gccttcaggc
      421 cgctggccgt caccggcgca cgacgccatc cgctgctgcc cgcggtgccg acgttcaagg
      481 agtcgggctt cgacggtttc gaaggcgtca cctggtacgg catcgtcggc ccggccggac
      541 tgccggacgc gatcgtgcgg cgcctgcatg aagaggtcgt caaggcgctc gcagcgcccg
      601 atctgcgcga gacgtttgca gcgcaggcga tcgatgtgat gccgatgacg acggcgcagt
      661 tcggccagta catcgccgac gagatcgcgc actggacagc ggtcgcgcgg gccagcaaga
      721 tcgaaggcga ctgaatcccc acttcacctg ccatgaccct gccctcgatc tccgtgtcgc
      781 agcgcctcgc cgccgcgttc gcggggcaga ccgcggcgct gccacccgcc gtggcgcaac
      841 tgtgcgatgc ggtgctgatg gacgtgaccg gcctgtgtgt ggcggctcgg cacagcgact
      901 acgtgcgggc cacgctgcag gcgaccgcgg ggcccggagt ctgtaccgcc atcggtcatg
      961 ccggcagctt caatgtggcg acggccgcgc tgtgcaacgg cacggcggcg cacggcgagg
     1021 attacgacga caccttcgtg ggcggcccgg tgcacgccgg cgccgtgatc gtcccggccg
     1081 tgctcgctgc ggccgaggcg cacggcctgt gcggcgccga tgtcgagcgg ggcatcgccg
     1141 tcggctgcga gctgatgtgc cggctgtgcc gggtggcgcc caagcgcgtg caccaggcgg
     1201 gctttcatcc gaccgcggtg ttcggtgcgc tgggggcggc cgccggcgtc gcttcggcga
     1261 tgcggctgga cctcaccgcc tggtgccatg ccttgggcat cgccggcagc ctggcgtcgg
     1321 gcatcatcga ataccttgcc gaaggcgcat ggaccaagcg catgcatccg ggctgggcgg
     1381 cacaggcggg ctatcgtgcc gcccgcctcg cacaggcggg cttcaccgga ccgcgcacgc
     1441 tgttcgacgg cgagcacggc ttcttccacg ccttcgccaa cagcgacggc tgcgatttca
     1501 gctccatgct ggacggcgcg ggccacgaat ggctgtgcag cgacatcgcg ttcaagccct
     1561 atgcctgcgg caccatggcc cacccctaca tcgattgcgc gcgccgactg gtcggcgaag
     1621 ggctggaccc gaacgacgtg gcgagcatcg agtgcaagac cgccgaaggc atcgtccatc
     1681 ggctgtggga gccgctcgag gccaagcaga acccggccaa cggctatgcc gcgaagttca
     1741 gcattcccta cgcgatcgcg gtcggaatgc tgcgcggcga cgccggcctc ggcgactacg
     1801 gggaggcggt cgtgcacgat ccggccgtgc gccggctggc cggccgggtg cgctacgtga
     1861 tcgacccgga caacccctat ccgcgccaat tcaccggcca tctgcgcgtg gcgctgcaca
     1921 gcggcgaggt gcgcgaggcg agccaggggc actttcgtgg cggccgcgac gaaccgatgt
     1981 cgccggcgac actcgagtcg aagtttctcg ccaactgtgc ctacggcggc tgggacgggc
     2041 agcgcgcgcg gcgggcgctc gctgtcttgc ggggcctgcg cgccgccccg cgcgtggcgc
     2101 tggacgcgct gcgcgcctag acgtcgcgac gagatgccca tggacgccgc accgctgaag
     2161 ggtcgggtcg cgctggtcac cggggccggc cgcaacatcg ggcgcgcaat cgcgctggcg
     2221 ttggccgacg cgggtgcgca ggtcgcggtc aacgtgcgcg ccagccggct ggaaggccag
     2281 ggggtggtcg acgagatcct cgcccgcggc ggcgacgcgt tgctggcgct cgccgacgtg
     2341 acccagcgtg cccaggtcga cgcgatggtc gcggcgatca cgcagcgctt cggccggctg
     2401 gacatcgtcg tcaacaacgc ggcgatccgg ttggaggcgc cgttcgacga gctgcgctat
     2461 gccgactggc aggctgccca cgcggtctgc gtcgacggcg ccttccattg cacgcaatcc
     2521 gcgttgccgc acctgcgccg cagcgatgcc ggcagcgtga tcaacatcgg cggcatgacg
     2581 gcgcacaccg gggcaatcca ccgcgcgcac gtggtgtcgg ccaaggcggc gctcggcggc
     2641 ctcacgcggg cgctggcgca cgaactggcc gactcggcga tcaccgtcaa ctgcgtgtcg
     2701 ccggggctga tcgacaccgt gcgccaacc
//
LOCUS       DASRDG010000243         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1297793, whole genome shotgun sequence.
ACCESSION   DASRDG010000243 DASRDG010000000
VERSION     DASRDG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297793"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /gene="xdhA"
                     /locus_tag="VFA35_04030"
     CDS             complement(<1..1433)
                     /gene="xdhA"
                     /locus_tag="VFA35_04030"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HZT55372.1"
                     /translation="MHHTLSPRPIRFFHRGAVVEVSDAAPTRTVLDWLREDARCTGTK
                     EGCNEGDCGACTVVIGETAAAGEPGAVRGLRLETVNACIQFLPTLDGKALFTVEDLKA
                     MTPDAALHPVQQALVECHGSQCGFCTPGFVMSLWSSYEHGRACGAQPTRQQLADALSG
                     NLCRCTGYRPILDAGQRMFELPPVALDTAPVIEALKGMAADATFTYAGRSADTFHAPT
                     TLAELAALREARPDAQLLAGSTDIGLWVNKQFRPLGDIIFVGKVIELKRIEARPDELY
                     IGAGASLEAAWAALAQRWPALTDVWLRFASPPIRHAGTMGGNVANGSPIGDSAPVLMA
                     LDAQLELRKGARVRCLPLATFYLDYMKNRLEPGEFVQGLAVPLAVMSRQVRAYKISKR
                     FDCDISAVCAGFSIELDGGLVKTVRLAYGGMAATVKRGKGAEAAIVGQPWTQATVNAA
                     QAALAQDFQPLSDMRASAAYRLQVARNL"
     gene            1529..2527
                     /locus_tag="VFA35_04035"
     CDS             1529..2527
                     /locus_tag="VFA35_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZT55373.1"
                     /translation="MTQRVDFDKIELYLIRVLKTLITERSVSRAAMRLQSTQPAVSAQ
                     LKRLRALTGDPLLVRAGNNMAPTDTALRLLEPATRLLQDADRLFSPRARQRAFDPLAS
                     GTTFRIAASDYLDPLFLPELVAHLKRAAPKVRLELLPLSGEFDYRRSLAAGEVDLVIG
                     NWLKPPEELHLGRLLSDEIVCLVAEDHPVVRAASTRAWSPERYLGCEHVAPTPLHAGV
                     GGAAPGVIDEHLASIGVTRDVMVRSAHFGLIPLMVAQSLLVLTTGRLFCSRYVDSLPV
                     RIVRCPMPFPPLTYYQLWHELTHASASGRWLRDQVRDVARNLAGHGMLRRVEGSPT"
     gene            2524..>2847
                     /locus_tag="VFA35_04040"
     CDS             2524..>2847
                     /locus_tag="VFA35_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348463.1"
                     /note="Catalyzes the deamination of guanine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HZT55374.1"
                     /translation="MSSNSRIALRGDLLDFTHEPGWGEVESDAVRFRPDHWLLIENGR
                     IQGAQRAEPDAGWQRHDHAGKLILPGFIDTHVHSPQLDVIASYGTELLDWLTTHTFPA
                     EARHAD"
BASE COUNT          396 a         1021 c         1012 g          418 t
ORIGIN      
        1 aggttgcgcg cgacctgcag ccggtacgcg gcgctcgcgc gcatgtccga gagcggttgg
       61 aagtcctgcg ccagtgccgc ctgcgcggcg ttcacggtcg cctgtgtcca gggctgaccg
      121 acgatcgcgg cctcggcccc cttgccgcgc ttcacggtcg cggccatgcc gccgtaggcc
      181 aggcgcacgg tcttgacgag gccgccgtcg agctcgatcg agaagcccgc gcacacggcc
      241 gagatgtcgc aatcgaagcg cttgctgatc ttgtacgcgc gcacctggcg cgacatgacg
      301 gcgagcggca cggccagccc ctgcacgaac tcgccgggct cgagccggtt cttcatgtag
      361 tcgaggtaga acgtggccag cggcaggcag cgcacgcgcg cgcctttgcg cagctcgagc
      421 tgcgcgtcga gcgccatcag caccggcgcc gaatcgccga tcggcgagcc gttcgcgacg
      481 ttgccgccca tcgtgccggc gtggcggatc ggcggtgacg cgaagcgcag ccacacgtcg
      541 gtcagcgccg gccagcgctg cgcgagcgcg gcccacgcgg cttcgagcga ggcgccggcg
      601 ccgatgtaga gctcgtcggg ccgcgcttcg atgcgcttca gctcgatgac tttgccgacg
      661 aagatgatgt cgccgagcgg gcggaactgc ttgttgaccc acaggccgat gtcggtcgag
      721 ccggcgagca gctgcgcgtc cggcctggcc tcgcgcagcg cggcgagttc ggccagcgtg
      781 gtcggcgcgt ggaaggtgtc ggccgatcgg cccgcgtagg tgaacgtcgc gtcggcggcc
      841 atgcccttca gcgcttcgat caccggtgcc gtgtcgagcg cgaccggcgg cagctcgaac
      901 atgcgctgcc cggcgtcgag gatcggccgg tagccggtgc agcggcacag gttgcccgag
      961 agcgcgtcgg cgagctgctg gcgcgtcggc tgcgccccgc acgcgcggcc gtgctcgtag
     1021 ctcgaccaca gcgacatcac gaagcccggc gtgcagaacc cgcactgcga gccgtggcac
     1081 tcgaccagcg cctgctggac cgggtggagc gccgcgtcag gcgtcatggc cttcagatcc
     1141 tcgaccgtga acagcgcctt gccgtcgagc gtcggcagga actggatgca ggcgttcacc
     1201 gtctccaggc gcaggccgcg caccgcgccg ggctcgcccg cggccgccgt ctcgccgatc
     1261 accaccgtgc aggcgccgca gtcgccttcg ttgcagccct ccttggtgcc ggtgcagcgc
     1321 gcgtcctcgc gcagccagtc gagcacggtg cgcgtcggcg ccgcgtcgct cacctcgacc
     1381 acggccccgc ggtggaagaa gcggatcggg cgggggctca gcgtgtggtg catggggggc
     1441 ggtcccgggt tgggcgatac ccgaaactta gtcgcttccg ggcatgtggg tatacagtgc
     1501 aaagcatagt gcttatgggt ctgggcttat gacgcagcgc gtggacttcg acaagatcga
     1561 gctctacctc atccgggtgt tgaagacctt gatcaccgaa cgcagtgtct cccgagccgc
     1621 gatgcgcctg caaagcacgc agcccgccgt gagtgcccag ctgaagcggc tgcgcgcact
     1681 gacgggtgac ccgttgctgg tgcgcgcggg caacaacatg gcgcccaccg acaccgcgct
     1741 gcgcctgctc gagccggcga cgcggctgct gcaagatgcg gaccggctgt tcagcccgcg
     1801 cgcccggcag cgcgccttcg acccgctggc cagcggcacg accttccgca tcgcggcgag
     1861 cgactacctc gacccgctgt tcctgcccga gctcgtcgcg cacctgaagc gggccgcgcc
     1921 gaaggtgcgg ctcgagctgc tgccgctgtc gggcgagttc gactaccggc gcagcctcgc
     1981 ggccggcgag gtcgatctgg tgatcggcaa ctggctgaag ccgccggagg aactgcacct
     2041 cgggcggctg ctgtcggacg agatcgtctg cctggtcgcc gaggaccacc cggtcgttcg
     2101 cgccgccagc acccgcgcct ggtcgcccga gcgctacctc ggctgcgagc acgtcgcgcc
     2161 gacgccgctg cacgcggggg tcggcggcgc ggccccgggc gtgatcgacg agcacctcgc
     2221 gtcgatcggc gtcacgcgcg acgtgatggt gcgaagcgcg cacttcgggc tgatcccgct
     2281 gatggtggcg cagagcttgc tggtgctgac caccggccgg ctgttctgtt cgcgctacgt
     2341 cgacagcctg ccggtgcgca tcgtgcgctg cccgatgccg ttcccgccgc tgacctacta
     2401 ccaactctgg cacgagctga cgcacgcgtc ggcctcgggc cgctggctgc gcgatcaggt
     2461 gcgtgacgtg gcgcgcaacc tggcggggca cggcatgctg cgacgtgtgg aggggtcccc
     2521 gacatgagct cgaactcgcg catcgcactg cgcggcgacc tgctcgactt cacccacgag
     2581 ccgggctggg gcgaggtcga gtccgacgcg gtgcgctttc ggcccgacca ctggctgctg
     2641 atcgagaacg gccgcatcca gggcgcgcag cgcgccgagc ccgacgccgg ctggcagcgc
     2701 cacgaccacg cgggcaagct gatcctgccc ggcttcatcg acacccacgt gcacagcccg
     2761 cagctcgacg tgatcgccag ctacggcacc gagctgctcg actggctgac gacccacacc
     2821 ttcccggccg aggcgcgcca cgccgac
//
LOCUS       DASRDG010000244         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1548386, whole genome shotgun sequence.
ACCESSION   DASRDG010000244 DASRDG010000000
VERSION     DASRDG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548386"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFA35_04045"
     CDS             <1..636
                     /locus_tag="VFA35_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZT55375.1"
                     /translation="TQIEPHDAIAELFDALVRTHTILIDLARDIWGYVSLGYFKQKTR
                     PGEVGSSTMPHKVNPIDFENAEGNFGLANALLTHLSQKLPISRWQRDLTDSTVLRNMG
                     VALGYALLGYDSLLRGLNKLEVNEAALAADLDAAWEVLAEPIQTVMRRYALPNPYERL
                     KELTRGKAITREAIAAFIETLEIPAAERARLLAMTPGSYVGQAVELAARIG"
     gene            706..975
                     /locus_tag="VFA35_04050"
     CDS             706..975
                     /locus_tag="VFA35_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZT55376.1"
                     /translation="MRIEFDSAKDASNQAKHGVSLALAGELDWEAALVWVDDRFEYAE
                     LRMIALAPKTEILYCVAFVDRGAARRIISLRRANRREVKHYVENL"
     gene            992..1264
                     /locus_tag="VFA35_04055"
     CDS             992..1264
                     /locus_tag="VFA35_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HZT55377.1"
                     /translation="MPTSSADKAITAAAKADPDAQPLTAKQLKAMVPMRTLRGRPKSE
                     NPKQLVSVRYSQEVLTYFKSTGEGWQSRMDGVLREYVVRQSRRAQR"
     gene            1355..1720
                     /locus_tag="VFA35_04060"
     CDS             1355..1720
                     /locus_tag="VFA35_04060"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZT55378.1"
                     /translation="MNKQHILTEIARTTAANGGIALGRERFYRETGSTTEAIGFVYLL
                     KTTKYFKVGRSSSFERRSRELAIQLPEKAETVHVIRTDDPVGIEPYWRRRFESKRRNG
                     EWFELNAQEVKAFKRRKFM"
     gene            1758..>2044
                     /locus_tag="VFA35_04065"
     CDS             1758..>2044
                     /locus_tag="VFA35_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HZT55379.1"
                     /translation="MNFTQQLARAEKANDSMLCVGLDPDPAKFPGSWKDDPNRIFDFC
                     ATIVDATKDLVIAFKPQIAYFAAQRAEDQLERLIAHIHKAAPAVPVILDAKR"
BASE COUNT          420 a          658 c          631 g          335 t
ORIGIN      
        1 acgcagatcg agccgcacga cgcgatcgcc gagctgttcg atgcgctggt ccgcacccac
       61 acgatcctga tcgacttggc gcgcgacatc tggggctacg tcagcctcgg ctacttcaag
      121 cagaagacgc ggccgggcga ggtcggctcg tcgacgatgc cgcacaaggt caacccgatc
      181 gacttcgaga acgccgaggg caacttcggc ctcgccaatg cgctgctcac gcacctgagc
      241 cagaagctgc cgatcagtcg ctggcagcgc gacctgaccg acagcacggt gctgcgcaac
      301 atgggcgtcg cgctcggcta cgcgctgctc ggctacgact cgctgctgcg cggcctgaac
      361 aagctcgagg tcaacgaagc cgcgctcgcc gccgacctcg acgccgcctg ggaagtgctg
      421 gccgaaccga tccagaccgt gatgcgccgc tacgcgctgc cgaaccccta cgagcgcctg
      481 aaggagctga cgcgcggcaa ggccatcacg cgcgaggcga tcgcggcgtt catcgagacg
      541 ctggagattc ccgcagcgga acgcgcgcgg ctgctggcaa tgacgccggg gagttatgtc
      601 gggcaggcgg tcgaattggc cgcgcgcatc gggtgagagt ctggtgctcg ggtcgggcga
      661 ttggacggcg tttgacaggt caatttactg tagctacagt aaagaatgcg aatcgagttc
      721 gattcagcca aagacgcgag caaccaagcg aagcacggtg tctcgctggc cttggctggc
      781 gagctcgatt gggaagccgc gctggtttgg gttgacgacc gcttcgagta cgccgagttg
      841 cgcatgatcg cgctggcacc gaagaccgag atcctttact gcgtggcatt tgtggatcgc
      901 ggcgcagcgc gaagaatcat cagcctgcgc cgcgccaatc gtcgcgaggt caaacactat
      961 gtcgaaaatc tctaggcgcc cggtcatcgc gatgccgact tcgtcggcgg acaaggcgat
     1021 cacggccgcc gccaaggccg acccggacgc gcagccgctc acggcgaagc agttgaaggc
     1081 gatggttccg atgcgaacgt tgcgcggccg cccgaagtcc gagaacccga agcagctggt
     1141 ctcggttcgc tacagtcaag aggttctgac gtacttcaaa tcgaccggtg agggttggca
     1201 atcccgcatg gacggcgtgc tgcgcgaata cgtcgtccga cagtcccgca gagcgcaacg
     1261 atgacgccgc gctgttctcc ggcgaccgac cccactccag caggcttcgc ctacctccgc
     1321 ggtccgcagc gtcacgcgtc ttagcctcgt ctccatgaac aagcagcaca tcctgaccga
     1381 gatcgcaaga acgacagcag caaacggtgg catcgccctt ggacgagaga ggttctatcg
     1441 tgagacaggc tcgaccacag aggccattgg cttcgtgtat ctcttgaaga ctacgaagta
     1501 cttcaaggtc ggtcgcagta gctcctttga gcgtcgttcg cgcgagcttg cgattcagct
     1561 acccgagaag gccgagacag ttcacgtcat tcgcaccgac gatccagtcg gaatcgagcc
     1621 gtattggcgc cgccgcttcg agtcgaagag aaggaacggt gagtggttcg aactgaatgc
     1681 gcaagaagta aaggcattca aacggcgaaa gttcatgtga aagacgccta acctcccatc
     1741 aagcggacat tcgaccgatg aacttcaccc aacaactcgc ccgcgccgaa aaggccaacg
     1801 actcgatgct ctgcgtcggc ctcgaccccg acccggccaa gttccccggc tcgtggaagg
     1861 acgacccgaa ccgcatcttc gacttctgcg cgaccatcgt cgacgccacc aaggacctgg
     1921 tgatcgcgtt caagcctcag atcgcctact tcgccgcgca gcgcgccgag gaccagctcg
     1981 agcgcctgat cgcgcacatc cacaaggccg cgcccgcggt gccggtgatc ctcgacgcga
     2041 agcg
//
LOCUS       DASRDG010000245         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_38322, whole genome shotgun sequence.
ACCESSION   DASRDG010000245 DASRDG010000000
VERSION     DASRDG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38322"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1194
                     /gene="sucC"
                     /locus_tag="VFA35_04070"
     CDS             31..1194
                     /gene="sucC"
                     /locus_tag="VFA35_04070"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZT55380.1"
                     /translation="MKIHEYQGKEILRQFGVPVPRGYPAFSVREASEAAQRLGGPVWV
                     VKAQIHAGGRGKGGGVKLARSMADVEKLSGEILGMQLKTHQTGPEGQKVRRLLIEEGA
                     DIKKEYYVAAVTDRATQQVAMMASSEGGMDIEKVAHDTPEKILKVFIDPATGLTDAQG
                     AELANGIGVPAASQAQAIDVFKKLYQCYMDTDCSLAEINPLILEGNGNIKALDAKFNF
                     DSNALFRHPEIVGYRDLDEEDPAEIEASKFDLAYIQLDGNIGCLVNGAGLAMATMDTI
                     KLFGGEPANFLDVGGGATAEKVTEAFKIMLGNPKVKAILVNIFGGIMRCDTIAEGVVA
                     ACKAVNLNVPLVVRMKGTNEDLGKKILKDSGLPIISAESMADAAKAVMAALGK"
     gene            1210..2103
                     /gene="sucD"
                     /locus_tag="VFA35_04075"
     CDS             1210..2103
                     /gene="sucD"
                     /locus_tag="VFA35_04075"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZT55381.1"
                     /translation="MSILIDKNTKVITQGITGKTGQFHTRMCRDYANGKNCFVAGVNP
                     KKAGEDFEGIPIFANVTEAAKATGATVSVIYVPPAGAAAAIWEAVEADLDLAICITEG
                     IPVKDMLIVRNKMKEKEAKGGKKTLLLGPNCPGLITPDEIKIGIMPGHIHMAGRVGVV
                     SRSGTLTYEAVAQLSELGIGQSTAVGIGGDPINGLKHIDIMRLFNDDPNTDAVIMIGE
                     IGGPDEAEAARWCQKHMKKPIVGFIAGVTAPAGKRMGHAGALISGGADTADAKLAVME
                     ECGFTVTRNPSEMGKLLKALL"
     gene            2123..2806
                     /locus_tag="VFA35_04080"
     CDS             2123..2806
                     /locus_tag="VFA35_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZT55382.1"
                     /translation="MDLTSGAFWAALGSIILANVVLSGDNAVVIAMAARALPAQQQKK
                     AIFWGSAAAIVMRIVLTLIAVEMLKWPYLKIVGALLLLYIGISLLAEDDGEGDHQEIS
                     GMAAAIRTILVADLVMSLDNVLAVAAAANGNTPLLIIGLAVSIPLIIFGSTLLLKVME
                     RFPIIITLGAALLGFLAGEMLLTDPAVEARFGELSHSVVNAAGAIGAVIVVAAGLWVQ
                     RRARAKAAA"
     gene            3044..>3710
                     /locus_tag="VFA35_04085"
     CDS             3044..>3710
                     /locus_tag="VFA35_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZT55383.1"
                     /translation="MELFSAGWLSALLAIVLIDLVLAGDNAIVIALAARKLPAHLQTK
                     AIVWGTVGAIVVRTAMTVGVVWLLKIPGLMLLGGLGLVWIAYKLLAPDGGGPSHEPSA
                     TTFWGAMKTIVIADALMGVDNVLGVAGAAKGSIDLVVIGLAVSVPIVVWGSTLVLKLV
                     ERFSVIVYAGAGVLAYTAAHMIVSEPLLDDLFDPHAPIRIATHAVLIVGVLGAGWWTT
                     HRAA"
BASE COUNT          677 a         1231 c         1231 g          571 t
ORIGIN      
        1 ccgaaaactg acaatccgag agacacgacc atgaagatcc acgagtacca aggcaaggaa
       61 atcctgcgcc agttcggcgt gccggtgccg cgcggctacc ccgcgttcag cgtgcgcgag
      121 gccagcgagg ccgcgcaaag gctcggcggc ccggtctggg tcgtgaaggc gcagatccac
      181 gccggcggtc gcggcaaggg tggcggcgtg aagctggcgc gttcgatggc cgatgtcgag
      241 aagctgtcgg gcgagatcct cggcatgcag ttgaagaccc accagaccgg ccccgagggt
      301 cagaaggtgc gccgccttct aatcgaggag ggcgcggaca tcaagaagga gtactacgtc
      361 gccgccgtca ccgaccgcgc gacgcagcag gtcgcgatga tggccagctc cgaaggcggg
      421 atggacatcg agaaagtggc ccacgacacg cccgagaaga tcctgaaggt tttcatcgac
      481 ccggccaccg gcttgaccga cgcgcaaggc gccgaactcg cgaacggcat cggcgttccg
      541 gccgcgagcc aggcgcaggc gatcgacgtg ttcaagaagc tctaccagtg ctacatggac
      601 accgactgct cgctcgcgga gatcaacccg ttgatcctgg aaggcaacgg caacatcaag
      661 gcgctcgacg ccaagttcaa cttcgactcg aatgccctct tccgccaccc cgagatcgtc
      721 gggtaccgcg atctggatga agaagacccg gccgagatcg aagccagcaa gttcgacctc
      781 gcctacatcc agctcgacgg caatatcggc tgcctggtca acggcgccgg cctggcgatg
      841 gcgacgatgg acacgatcaa gctgttcggc ggcgagccgg cgaactttct cgacgtcggc
      901 ggcggcgcga ccgccgagaa ggtcaccgag gccttcaaga tcatgctcgg caacccgaag
      961 gtcaaggcga tcctggtcaa catcttcggc ggcatcatgc gctgcgacac gatcgccgaa
     1021 ggcgtggtcg ccgcgtgcaa ggcggtcaac ctcaacgtgc cgctggtggt gcgcatgaag
     1081 ggcaccaacg aagacctggg caagaagatc ctgaaggact cgggcctgcc gatcatcagc
     1141 gccgagtcga tggccgacgc cgccaaggcc gtgatggccg cgctcggcaa gtaagaagag
     1201 gaaatcccca tgagcatcct gatcgacaag aacaccaagg tcatcaccca gggcatcacc
     1261 ggcaagaccg gccagttcca cacccgcatg tgccgcgact acgcgaacgg caagaactgc
     1321 ttcgtcgccg gcgtgaaccc gaagaaggcc ggcgaagact tcgaaggcat cccgatcttc
     1381 gcgaacgtga ccgaggcggc caaggccacc ggcgcgaccg tcagcgtcat ctacgtgccg
     1441 ccggccggcg ccgccgcggc gatctgggag gcggtcgagg ccgacctcga cctggcgatc
     1501 tgcatcaccg aaggcatccc ggtgaaagac atgctgatcg tgcgcaacaa gatgaaggaa
     1561 aaggaggcca agggcggcaa gaagacgctg ctgctcgggc cgaactgccc cgggctgatc
     1621 acgcccgacg agatcaagat cggcatcatg ccgggccaca tccacatggc cggccgcgtc
     1681 ggcgtggtct cgcgctcggg cacgctcaca tatgaagcgg tcgcgcagct cagcgagttg
     1741 ggcatcggcc agtcgaccgc ggtcggcatc ggcggcgacc cgatcaacgg gctgaagcac
     1801 atcgacatca tgcggctctt caacgacgac ccgaacaccg acgcggtgat catgatcggc
     1861 gagatcggcg gccccgacga agccgaggcc gcgcgctggt gccagaagca catgaagaag
     1921 ccgatcgtcg gtttcatcgc cggcgtgacc gcgccggccg gcaagcgcat gggccacgcc
     1981 ggcgcgctga tttccggcgg cgccgacacg gcggacgcca agctcgcggt gatggaggag
     2041 tgcggcttca ccgtcacgcg caacccgtcc gagatgggca agctgctcaa ggccttactg
     2101 tgacgcaggg ctgacgcgtc gcatggacct cacctcaggc gccttctggg ccgcgctcgg
     2161 ttcgatcatc ctcgccaacg tcgtgctgtc gggcgacaac gccgtcgtga tcgcgatggc
     2221 ggcgcgggcg ttgccggcgc agcagcagaa gaaggcgatc ttctggggca gcgccgcggc
     2281 gatcgtgatg cggatcgtgc tgacgctgat cgcggtcgag atgctgaagt ggccgtacct
     2341 gaagatcgtc ggcgcgctgc tgctgctcta catcggcatc tcgctgctcg ccgaggacga
     2401 cggcgaaggc gaccaccagg agatcagcgg catggccgcg gcgatccgca cgatcctcgt
     2461 tgccgacctg gtgatgagcc tggacaacgt gctcgcggtc gccgccgcgg ccaacggaaa
     2521 caccccgctg ctgatcatcg gcctggccgt cagcatcccg ttgatcatct tcggcagcac
     2581 cttgctgctc aaggtgatgg agcgtttccc gatcatcatc acgctcggcg cggcgctgct
     2641 cggttttctc gcgggcgaga tgctgctgac cgacccggcc gtcgaggcgc gcttcggcga
     2701 gctgtcgcac agcgtcgtca acgcggctgg cgcgatcggc gcggtgatcg tcgtcgcggc
     2761 cgggctctgg gtgcagcgcc gtgcgcgcgc aaaagccgcg gcttgacatg ggcagggacg
     2821 ccctgtccta atgaggcttg aaggggagta gctcaatgtt cgccgggtgc cagcgtcgct
     2881 ggagtcggcc aacgtttcgg tgtgccgtca agacgaagcg gggtccacga cccgcttccg
     2941 gtgcaccggg ccacaacatt cactccatgc cgggcaagac cttcgatgca agcagttgct
     3001 tgcatcgttc gtcgtgcatc gctgctgtcc tcatggagac cgtatggaac tcttctctgc
     3061 cggctggctg tccgccttgc tggccatcgt cctgatcgac ctcgtgctcg ccggcgacaa
     3121 cgcgatcgtg atcgcgctcg ccgcgcgcaa gcttcccgcc catctgcaaa ccaaggccat
     3181 cgtctggggc accgtcggcg cgatcgtcgt gcgcaccgcg atgaccgtcg gcgtcgtctg
     3241 gctgctgaag atccccggcc tgatgctgct cggcggcctc ggcctggtct ggatcgccta
     3301 caagctgctc gcgccggacg gcggcgggcc gtcgcacgaa ccgagcgcga ccacgttctg
     3361 gggtgcgatg aagaccatcg tgatcgccga cgcgctgatg ggcgtcgaca acgtgctcgg
     3421 cgtcgccggc gccgccaagg gcagcatcga cctcgtcgtg atcggcctgg cggtcagcgt
     3481 gccgatcgtc gtctggggca gcacgctcgt gctcaagctg gtcgagcgct tctccgtcat
     3541 cgtctacgcc ggcgccggcg tgctcgccta caccgcggcg cacatgatcg tcagcgagcc
     3601 cttgctcgac gacctgttcg acccgcacgc cccgatccgc atcgccacgc atgccgtgct
     3661 gatcgtcggc gtgctcggtg cgggctggtg gaccacgcac cgcgccgcaa
//
LOCUS       DASRDG010000246         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_608106, whole genome shotgun sequence.
ACCESSION   DASRDG010000246 DASRDG010000000
VERSION     DASRDG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608106"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2342
                     /locus_tag="VFA35_04090"
     CDS             <1..2342
                     /locus_tag="VFA35_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZT55384.1"
                     /translation="QQIQVLAAESRSIEEQERALRNRRERLAGERQQLATPDLNKLAD
                     LRERTAQADALKDETEARLHECGEQVPMLDEQRRTQQDVLNRDQARQSDLTARLEALR
                     ALQEKVQTEGKLKPWLAKHGLDALQGLWTKIHIESGWETALEAALRERMGALEVGRLE
                     TIRAFANDAPPAKLAFYTPPQAAISNTHQTLSKLSDLLRLANSDQAGLKALLNDWLEG
                     VYTAANIDEALAERAKLTHGEVIMTRDGHAVSQYAVAFYAPDSEQAGMLARAQEIENL
                     ERQLRAQVLIAEEARSSLVRAEASYTDASQRLIGARREAGEAQTRAHQLQVELLRLSQ
                     LAEQTSTRRAQLDEELAEIDAQLEELAERGATGEARFEELDMQLATTQECHAELDDAV
                     IGAERRLAEAREQLRALERQSQEAQFAARSLAARRGELQRGIETAAQQLAANDQAHVS
                     LQEELTRLTDASAQAGLQEALALKLAREAALGAKRSEYDDLTLRLRRADEQRLQHEQS
                     LQPLRERITKLQLEEQAAQLGGAQYFDQLTAANADFEALAAGIEAGGVKLAGLQGEID
                     RINREINALGAVNLAALDELTASRERKTFLDAQNADLIEAMTTLEDAIHKIDLETRDL
                     LASTFDQVNTHFGRMFPELFGGGNAKLVMTGDEILDAGVQVMAQPPGKKNSTIHLLSG
                     GEKALTAIALVFAIFQLNPAPFCLLDEVDAPLDDANTERYAKLVKQMALSTQFLFISH
                     NKIAMEMAEQLIGVTMQEQGVSRVVAVDMDAAVSLAEAA"
BASE COUNT          436 a          848 c          820 g          261 t
ORIGIN      
        1 agcagcagat ccaggtcctc gcggccgaga gccgcagcat cgaggagcag gagcgcgcgc
       61 tgcgcaaccg gcgcgagcgc cttgccggcg agcgccagca gctcgccacg cccgacctga
      121 acaagctcgc cgacctgcgc gagcgcaccg cccaggccga tgcgctgaag gacgagaccg
      181 aggcgcgcct gcacgaatgc ggcgagcagg tgccgatgct cgacgagcag cgccgcacgc
      241 agcaggacgt cctgaaccgc gaccaggcgc gccagtccga cctcaccgcc cgcctcgagg
      301 cgctgcgcgc gctgcaggag aaggtgcaga ccgaaggcaa gctcaagccc tggctcgcga
      361 agcacgggct cgatgcgctg caggggctgt ggacgaagat ccacattgaa tctgggtggg
      421 agaccgcgct cgaagccgcg ctgcgcgagc gcatgggcgc actcgaagtg gggcggctgg
      481 agacgatccg cgcgttcgcg aacgacgcgc cgccggccaa gctcgcgttc tacacgcccc
      541 cgcaggcggc gatctcgaac acgcaccaga ccctgtccaa gctcagcgac ctgctgcgcc
      601 tggcgaattc cgatcaggcc ggcctgaagg cgctgctgaa cgactggctc gaaggcgtct
      661 acaccgcggc gaacatcgac gaggcgctgg ccgagcgcgc gaagctgacc cacggcgagg
      721 tcatcatgac ccgcgatggc catgccgtga gccagtacgc ggtcgcgttc tacgcgcccg
      781 actccgagca ggccggcatg ctggcgcgcg cgcaggagat cgagaacctc gagcgccagc
      841 tgcgcgccca ggtgctgatc gccgaggagg cgcgcagcag cctggtgcgc gccgaggcga
      901 gctacaccga cgcctcgcag cgcctgatcg gggcgcgccg cgaggcgggc gaggcgcaga
      961 cgcgcgcgca ccagctgcag gtcgagctgc tgcgcctgtc gcaactggcc gagcagacca
     1021 gcacgcgccg cgcgcagctc gacgaggagc tggccgagat cgacgcgcag ctcgaagaac
     1081 tcgccgagcg cggcgcgacc ggcgaggcgc gcttcgaaga gctcgacatg cagctcgcga
     1141 ccacgcagga gtgccacgcc gaactcgacg acgcggtgat aggcgccgaa cgccggctcg
     1201 ccgaggcgcg cgagcagctg cgcgcgctcg aacgccagtc gcaggaggcg cagttcgccg
     1261 cccgctcgct ggccgcgcgc cgcggcgaac tgcagcgcgg catcgagacc gcggcccagc
     1321 agctcgccgc caacgaccag gcccacgtct cgctgcagga agaactgacg cggctgaccg
     1381 acgcgtcggc ccaggccggc ctgcaggaag cgctcgcgct gaagctggcc cgcgaggccg
     1441 cgctcggtgc caagcgcagc gagtacgacg acctgacgct gcgcctgcgg cgcgccgacg
     1501 agcagcgcct gcagcacgag cagagcctgc agccgctgcg cgagcgcatc accaagctgc
     1561 agctcgaaga gcaggccgcg cagctcggcg gcgcgcagta cttcgaccag ctgacggcgg
     1621 cgaacgccga cttcgaggcg ctcgccgccg gcatcgaggc gggcggcgtc aagctcgccg
     1681 ggctgcaagg cgagatcgac cgcatcaacc gcgagatcaa tgcgctcggc gccgtcaacc
     1741 tcgccgcgct cgacgagctg accgcgtcgc gcgaacgcaa gaccttcctc gacgcgcaga
     1801 acgccgacct gatcgaggcg atgacgacgc tcgaagacgc gatccacaag atcgacctcg
     1861 aaacccgcga cctgctcgcc agcaccttcg accaggtcaa cacgcacttc ggccggatgt
     1921 tccccgagct cttcggcggc ggcaacgcga aattggtgat gaccggcgac gaaatcctcg
     1981 acgccggcgt gcaggtgatg gcgcagccgc ccggcaagaa gaacagcacg atccacctgc
     2041 tgtcgggcgg cgaaaaggcg ctgaccgcga tcgcgctcgt gtttgcgatc ttccagctga
     2101 acccggcgcc gttctgcctg ctcgacgagg tcgacgcgcc gctcgacgac gccaacaccg
     2161 agcgctacgc caagctcgtc aagcagatgg cgctctccac gcagttcctg ttcatcagcc
     2221 acaacaagat cgcgatggag atggccgagc agctgatcgg cgtgacgatg caggagcaag
     2281 gcgtctcgcg cgtggtggcg gtcgacatgg acgcggccgt cagcctggcg gaggccgcct
     2341 gaccatgagc gcgccagtgc tgcac
//
LOCUS       DASRDG010000247         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1548544, whole genome shotgun sequence.
ACCESSION   DASRDG010000247 DASRDG010000000
VERSION     DASRDG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548544"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /gene="clsB"
                     /locus_tag="VFA35_04095"
     CDS             complement(<1..1184)
                     /gene="clsB"
                     /locus_tag="VFA35_04095"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HZT55385.1"
                     /translation="MNAPARTRALRPDHRLTLLKGGEALFPALVEAIAAARTEVLFET
                     YLFEFAGSVIPVAEAIEAAARRGVCVRVVVDGVGTGDVPAEWQRRWQAAGVHWRVFNP
                     ARGWRLLLPKRWRRLHRKLCVVDGRVGFCGGINLLDDFFDPNHGALAQPRFDFAVRVD
                     GPLVVDMHDTMTRLWARLQVAREARQRDFGGALQAVLEAAHAGTDMGDADLERDVERL
                     PAPGSEQGALAALVLRDNVRFRRSIEGIYRLAIGLARSEILIANAYFVPGVQLQRALL
                     RAARRGVRVTLLLQGRYEYFMQFYASRAVYAAMLEAGIEIIEYEASFLHAKVAVMDAP
                     DGAIATVGSSNLDPLSLLLAREANVFVRDDAFAAELRTHLVDAIAQGRRVAPDAITRW
                     SP"
     gene            complement(1181..1975)
                     /locus_tag="VFA35_04100"
     CDS             complement(1181..1975)
                     /locus_tag="VFA35_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZT55386.1"
                     /translation="MRHSTLHTSMFPGDVLRVATYNIHKGVRGVGPRKRLEIHNLALG
                     IEALDTDLVFLQEVRAVNRAEARKFPDTVIGWPRMPQADYLAPEGYEVAYRTNAKTTH
                     GEHGNALLSRWPLGDIGHHDVSDHRFEQRGLLHVPVNWNGTMVHAIVAHFGLIHASRV
                     RQAERLARFIEAEVPKKEPLIVAGDFNDWGLKLDEPMRQFGLRRAGKPGDPPARFNTF
                     PSRLPVFSMDRIYLRGLQCLSTSVPRGAAWARMSDHLPLIAELTRG"
     gene            complement(2059..>2158)
                     /locus_tag="VFA35_04105"
     CDS             complement(2059..>2158)
                     /locus_tag="VFA35_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HZT55387.1"
                     /translation="HLRHEWLDWRAAADKCYSPSNAEAVLQLPRFS"
BASE COUNT          322 a          768 c          770 g          298 t
ORIGIN      
        1 atgggcgacc agcgcgtgat cgcatcgggc gcgacacgcc ggccctgcgc gatcgcgtcc
       61 accaggtggg tgcgcagttc ggccgcgaag gcgtcgtcgc gcacgaagac gttcgcctcg
      121 cgcgcgagca gcaggctcag cgggtcgagg ttcgacgagc cgacggtggc gatcgcgcca
      181 tccggcgcgt ccatcaccgc gaccttggcg tgcaggaagc tcgcctcgta ctcgatgatc
      241 tcgatgcccg cctccagcat tgcggcatag accgcgcggc tggcgtagaa ctgcatgaag
      301 tactcgtagc gcccctgcag caacagcgtg acgcgcacgc ctcgccgcgc cgcgcgcagc
      361 agcgcgcgct gcaactgcac gccggggacg aagtaggcgt tggcgatcag gatctcgctt
      421 cgcgccagcc cgatcgcgag ccggtagatg ccctcgatgc tgcgccggaa gcgcacgttg
      481 tcgcgcagca ccagcgcggc cagcgcgccc tgctccgacc ccggcgccgg cagccgctcg
      541 acatcgcgct ccaggtcggc atcgcccatg tcggtgccgg catgggccgc ctcgagcacc
      601 gcctgcagcg cgccgccgaa gtcgcgctgg cgcgcctcgc gcgcgacctg caggcgcgcc
      661 cacaggcgcg tcatcgtgtc gtgcatgtcg acgaccagcg ggccgtcgac gcgcaccgcg
      721 aagtcgaagc gcggctgggc gagcgcgccg tggttcgggt cgaagaagtc gtcgagcagg
      781 ttgatgccgc cgcagaagcc gacgcggccg tcgaccacgc acagcttgcg gtgcagccgg
      841 cgccagcgct tgggcagcag caggcgccag ccgcgcgccg ggttgaacac gcgccagtgc
      901 acgccggcgg cctgccagcg ccgttgccac tcggccggca cgtcgccggt gccgacgccg
      961 tcgacgacga cccgcacgca cacgccgcgc cgcgccgcgg cctcgatcgc ctcggccacc
     1021 gggatcaccg agcccgcgaa ctcgaacagg taggtctcga acagcacctc ggtacgcgcc
     1081 gcggcgatcg cctcgaccag cgccgggaac aaggcctcgc cgcccttcag cagcgtcagc
     1141 cggtggtccg gacgcagcgc gcgggtgcgg gccggcgcgt tcatccgcgc gtcagttcgg
     1201 cgatcagcgg caggtggtcc gacatgcgcg cccaggccgc accacggggc accgaggtcg
     1261 acaggcactg cagcccgcgc aggtagatgc ggtccatcga gaacaccggc agccgcgacg
     1321 ggaaggtgtt gaagcgcgca ggcggatcgc ccggcttgcc ggcgcgtcgc aggccgaatt
     1381 gacgcatcgg ctcgtcgagc ttcaagcccc agtcgttgaa gtcgccggcg acgatcagcg
     1441 gttccttctt cggcacctcg gcctcgatga agcgcgccag ccgctcggcc tggcgcacgc
     1501 gactcgcatg gatcaacccg aagtgggcga cgatcgcgtg caccatggtg ccgttccagt
     1561 tcaccggcac gtgcagcaat ccgcgctgct cgaagcgatg gtcggacacg tcgtggtggc
     1621 cgatgtcgcc gagcggccag cgcgacagca gcgcgttgcc gtgctcgcca tgggtcgtct
     1681 tcgcgttggt gcggtacgcc acctcgtagc cctcgggcgc gaggtagtcg gcctgcggca
     1741 tgcgcggcca gccgatcacc gtatcgggaa acttgcgcgc ctcggcgcgg ttgacggcac
     1801 gcacctcctg caggaacacg aggtcggtgt cgagcgcctc gatgccgagc gcgaggttgt
     1861 ggatctccag ccgcttgcgc gggccgacgc cgcgcacgcc cttgtggatg ttgtaggtgg
     1921 ccacgcgcag cacgtcgccg gggaacatcg aggtgtgcag ggtgctgtgg cgcatgtcag
     1981 tggcgcccgg ccgtccgaag ccactgacgc gccccctcgg ggggcagcga acgaagtgag
     2041 cgtgggggtt ctctcatttc agctgaagcg agggagttgc agcacggctt cggcgttgga
     2101 cggcgagtag cacttgtccg ctgccgcgcg ccagtccagc cattcgtggc gcaggtgc
//
LOCUS       DASRDG010000248         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1190023, whole genome shotgun sequence.
ACCESSION   DASRDG010000248 DASRDG010000000
VERSION     DASRDG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190023"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..795)
                     /locus_tag="VFA35_04110"
     CDS             complement(22..795)
                     /locus_tag="VFA35_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55388.1"
                     /translation="MAPWIAFPHDNAEYTFDADTLRRHWARLHAGDAEPPPSDTKVLA
                     AWALFHAGEFRKATEAGLQAGGAGITVANKAQAIYANYLERSERAKLALFMEVAERAE
                     TQLATDPKNANAYYFMAYALGRYSHGISIAKALAQGLGSKVKSALETAIKLAPQHADA
                     HIALGAFQAEVIDKVGSLLGRTQGASKDAGLAMFKTALKLNPSSAIAMVEYANGLVML
                     EGDKKMKEATKLYEDAAACVPMDAMERLDVEMARAELAG"
     gene            complement(876..1541)
                     /locus_tag="VFA35_04115"
     CDS             complement(876..1541)
                     /locus_tag="VFA35_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZT55389.1"
                     /translation="MAKQTKKPRRTAERILEVTLALFNRFGEPNVSTTLISAELGISP
                     GNLYYHYPAKDELINSLFDRYEHALSELLRAADGVANVEDAWLLFHMLFELIWEYRFL
                     YRDLNDLLSKNRRLETHFQFVLKNKGKAVQQVLDGLARSNAMRMAAADVEPVATAMVV
                     VLTYWLSFEYVRDPRRALEPEAASAALLRGAFHVLSLLSPYLETAQREHLHTLVGNYK
                     KGD"
     gene            1659..>2550
                     /locus_tag="VFA35_04120"
     CDS             1659..>2550
                     /locus_tag="VFA35_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT55390.1"
                     /translation="MPAISFQNVAKTYLGKSGATRALDDVSFDIDAGEFFGLLGPNGA
                     GKTTLISILAGLARASSGTVSVLGHDVVKDYAAARRSLGIVPQELVFDPFFSVREALR
                     IQSGYFGVRHNDGWIDELLANLGLADKADANMRQLSGGMKRRVLVAQALVHRPPVIVL
                     DEPTAGVDVELRQTLWQFVARLNREGHTVLLTTHYLEEAEALCGRIAMLKLGRVVALD
                     RTSALLAGTASTMMRFKMDTPLPSELAAQSRVTGRIVQIKAHDAREVERVLAILRGAG
                     ARPEDLEIGRADLEDVFLEIM"
BASE COUNT          366 a          856 c          879 g          449 t
ORIGIN      
        1 ttcgcggcct ggccgagcgg gtcagcccgc cagctccgcc ctcgccatct ccacatccag
       61 ccgctccatc gcgtccatcg gcacgcatgc cgcggcgtct tcgtagagct tcgtcgcctc
      121 cttcatcttc ttgtcgcctt cgagcatcac gaggccgttc gcgtactcga ccattgcgat
      181 cgccgaactg gggttcagct tcagcgcggt cttgaacatc gcgaggccgg cgtccttgct
      241 cgcgccctgg gtgcggccga gcagcgagcc gaccttgtcg atcacctcgg cctggaaggc
      301 gccgagcgcg atgtgcgcgt cggcgtgttg cggcgcgagc ttgatcgcgg tttcgagcgc
      361 gctcttgacc ttgctgccca gcccctgcgc gagcgccttc gcgatggaga tgccgtggct
      421 gtagcggccg agcgcgtagg ccatgaagta gtaggcgttg gcgttcttcg ggtcggtggc
      481 gagctgcgtt tcggcgcgct cggccacctc catgaagagc gcgagcttgg ccctttcgct
      541 cctctcgagg tagttcgcgt agatggcctg cgccttgttc gcgacggtga tgcccgcccc
      601 gccggcctgc agcccggcct cggtcgcctt gcggaactcg ccggcgtgga acaacgccca
      661 cgcggccaac accttcgtgt cgctcggcgg cggctcggca tcgccggcgt gcaggcgtgc
      721 ccaatggcgc cgaagcgtgt ccgcatcgaa ggtgtactcg gcgttgtcgt gcggaaacgc
      781 gatccagggg gccatctcag tgccgcccgg ccgttccgaa ggtactgagc gcgccgtcgg
      841 ggggcagcga gcggagcgag cttgggggtt tcatcctagt ctcccttctt gtagtttccg
      901 acgagcgtgt gcaggtgttc gcgttgtgcg gtctcgaggt agggcgacag caggctcagc
      961 acgtggaagg cgccgcgcag cagcgccgcg cttgccgctt cgggctcgag cgcgcggcgc
     1021 gggtcgcgca cgtattcgaa gctcagccag taggtcagca cgacgaccat cgcggtcgcg
     1081 acgggttcga cgtcggcggc ggccatgcgc atcgcgttgc tgcgtgccag gccgtcgagc
     1141 acctgttgca ccgccttgcc cttgttcttc agcacgaact ggaagtgcgt ctcgagccgg
     1201 cggttcttcg agagcaggtc gttcaggtcg cggtacagga agcggtactc ccagatcagc
     1261 tcgaacagca tgtggaagag cagccaggcg tcttcgacgt ttgccacgcc gtcggccgca
     1321 cgcagcagct ccgacagcgc gtgctcgtag cggtcgaaca ggctgttgat cagctcgtcc
     1381 ttcgccgggt agtggtagta caggttgccg gggctgatgc cgagctcggc cgagatcagc
     1441 gtggtcgaca cgttcggctc gccgaagcgg ttgaacagcg ccagcgtgac ttcgagaatg
     1501 cgttcggccg tgcggcgcgg cttcttcgtt tgcttggcca tgtgtcgtcg agagtccccg
     1561 cgggcaatct agcatcaagc gttcgggtca cgcatcggca tcggccccgg ggtcgtcacg
     1621 cgcacgaagg cggcgcgctg cctacaatcc gcggccacat gccagccatc tccttccaga
     1681 acgtcgcgaa gacgtacctc ggcaaaagcg gcgcgacccg cgccctcgac gacgtgagct
     1741 tcgacatcga cgccggcgag ttcttcggcc tgctcgggcc gaacggcgcg ggcaagacca
     1801 cgctgatcag catcctggcc ggtctggccc gcgccagcag cggcacggtg agcgtgctcg
     1861 gccacgatgt ggtcaaggac tacgccgccg cgcgccgcag cctcggcatc gtgccgcagg
     1921 agctggtgtt cgacccgttc ttcagcgtgc gcgaggcgct gcgcatccag agcggctact
     1981 tcggtgtccg ccataacgat gggtggatcg acgagctgct cgcgaacctg ggtctggccg
     2041 acaaggccga cgccaacatg cgccagctct ccggcggcat gaagcgccgc gtgctggtcg
     2101 cgcaggcgct ggtgcaccgg ccgccggtga tcgtcctcga cgagcccacg gccggtgtcg
     2161 acgtcgaact gcgccagacg ctgtggcagt tcgtcgcgcg cctgaaccgc gaaggccaca
     2221 ccgtgctgct gaccacgcac tacctcgaag aggccgaggc gctgtgcggg cgcatcgcga
     2281 tgctcaagct gggccgcgtc gtcgcgctcg accgcacatc ggcgctgctg gcgggcaccg
     2341 ccagcacgat gatgcgcttc aagatggaca cgccgctgcc gagcgagctg gcggcgcagt
     2401 cgcgcgtcac cggccgcatc gtgcagatca aggcgcacga cgcacgcgag gtcgagcggg
     2461 tgcttgcgat cctgcggggt gccggtgcgc ggcccgagga tctggagatc ggccgtgccg
     2521 acctcgagga tgtcttcctc gagatcatgc
//
LOCUS       DASRDG010000249         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_268691, whole genome shotgun sequence.
ACCESSION   DASRDG010000249 DASRDG010000000
VERSION     DASRDG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268691"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFA35_04125"
     CDS             <1..561
                     /locus_tag="VFA35_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HZT55391.1"
                     /translation="DVNTGGFVGARNFDDTIFKTNLEASQAIARQLRLRNLGGIIIID
                     FIDMTREDHQGAVLAELRKQLARDRTKITVSGFTQLGLVEMTRKRTRESLAHMLCEVC
                     PVCEGRGQVKTPRSVCYDILREILREARQFNPKEFRIIASAAVVEMLLDEESQHLAGL
                     SDFIGKPISLQAEAMASPEQYDIVLM"
     gene            611..847
                     /locus_tag="VFA35_04130"
     CDS             611..847
                     /locus_tag="VFA35_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55392.1"
                     /translation="MPGREMKNITLSAEESLIEAARERARLEHTTLNEQFRRWLADYA
                     HTRERMQRYDAVMAKLRGRVKVGHKLTRDEMNER"
     gene            837..1262
                     /locus_tag="VFA35_04135"
     CDS             837..1262
                     /locus_tag="VFA35_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZT55393.1"
                     /translation="MSAEVFIDTNVFIYQLDKTDARKHKLAERIVRDGLAAGNACISF
                     QVVQECLNTVLRKAQVALDPESARSYLDTVLIPLLQVPASPALYHRALDVQGRWRFSF
                     YDSLIVAAALTAGCSRLLSEDLQHGQRIETLTIHNPFEH"
     gene            complement(1285..>2099)
                     /locus_tag="VFA35_04140"
     CDS             complement(1285..>2099)
                     /locus_tag="VFA35_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZT55394.1"
                     /translation="AVAGYAGPHTVRLNRNPVNLGIGAHLSRLVELSRGEMLFVTAGD
                     DVSLPARCERVMRSWQEHGRRPDLIATALTDIDAQGVDHGAIVPSDLADYRSAADWLA
                     RPPFVVGAAQAWTRRLFDRFGPLRPGVVGEDLILVLRAIGSGGAITLAEPLVRYRRGG
                     VSRRVRNLHAADVVARLRKSSRGALAELPQLLADTAVMGQLAAVEPTLRRRLARAQFV
                     HDVFAADTAGAKLRVLWRAHDVALATRLRIGVYAVCPGLYAPFFFVKRLVRR"
BASE COUNT          368 a          717 c          717 g          297 t
ORIGIN      
        1 gacgtcaaca ccggcggctt cgtcggcgcg cgcaacttcg acgacacgat cttcaagacc
       61 aacctcgagg cgagccaggc gatcgcgcgc cagctgcggc tgcgcaacct cggcggcatc
      121 atcatcatcg acttcatcga catgacgcgc gaggaccacc aaggcgcggt gcttgccgaa
      181 ctgcgcaagc agctcgcgcg cgaccgtacc aagatcaccg tgagcggctt cacccagctc
      241 gggctggtcg agatgacgcg caagcgcacg cgcgaatcgc tcgcgcacat gctgtgcgag
      301 gtctgcccgg tgtgcgaagg gcgcggtcag gtgaagacgc cgcgcagcgt ctgctacgac
      361 atcctgcgcg agatcctgcg tgaggcgcgc cagttcaacc cgaaggagtt ccgcatcatc
      421 gcctcggccg cggtcgtcga gatgctgctc gacgaagaga gccagcatct cgcggggctg
      481 agcgacttca tcggcaagcc gatctcgctg caggccgagg cgatggcgtc gccggagcag
      541 tacgacatcg tgttgatgtg aggcgccgtt gattgtgtac actgctttcg tgtacgcttg
      601 attcgtgtaa gtgccgggcc gcgagatgaa gaacatcaca ctgagcgccg aagagtctct
      661 gatcgaggcc gcgcgcgagc gtgcgcggct cgagcacacg accttgaacg aacagttccg
      721 ccgctggctg gccgactacg cccacacgcg cgagcgcatg cagcgttacg acgctgtgat
      781 ggccaagctg cgcggccgag tgaaggtcgg gcacaagctc acgcgggacg agatgaatga
      841 gcgctgaagt cttcatcgac accaacgtct tcatctatca actcgacaag accgacgccc
      901 gcaagcacaa gctggccgag cgcatcgttc gcgatggcct ggcggccggc aacgcgtgca
      961 tcagctttca ggtcgtgcag gagtgtctga acacggtgct gcgcaaggcg caagtcgcgc
     1021 tcgatcccga gtccgcacga agctacctcg acaccgtgct gatcccgctg ctgcaggttc
     1081 ccgccagccc ggcgctgtac caccgcgccc tggacgttca aggtcgctgg cgcttcagct
     1141 tctacgactc gctcatcgtg gccgccgcgc tgacggcagg gtgcagccga cttctgagcg
     1201 aagaccttca gcatggccag cgcatcgaga cgctgacgat ccacaacccg ttcgagcatt
     1261 gacgcgctgc tccgacggcg cggctcagcg ccgcacgagc cgcttgacga aaaagaacgg
     1321 ggcgtagagc cccgggcaga ccgcatagac gccgatgcgc aagcgcgtgg cgagcgcgac
     1381 atcgtgggcg cgccagagca cgcgcagctt cgcgccggcg gtgtcggcgg cgaacacgtc
     1441 gtgcacgaac tgcgcgcgcg ccagccgcct gcgcagcgtg ggttcgaccg ccgcgagctg
     1501 gcccatcacg gcggtgtcgg cgagcagctg cggcagctcg gcgagcgcgc cgcggctgct
     1561 cttgcgcagc cgcgcgacga cgtcggccgc atgcaggttg cgcacccggc gcgacacgcc
     1621 gccgcgccgg tagcgcacca gcggctcggc gagcgtgatc gcgccgccgg agccgatcgc
     1681 gcgcagcacc aggatcaggt cctcgccgac gacaccgggc cgcaacgggc cgaagcgatc
     1741 gaacaggcgg cgcgtccagg cctgcgcggc gccgacgacg aagggcgggc gcgccagcca
     1801 gtccgcggca ctgcggtagt ccgcgagatc gctcggcacg atcgcgccgt ggtcgacgcc
     1861 ctgcgcatcg atgtccgtca acgccgtggc gatcaggtcg ggccgccggc cgtgttcctg
     1921 ccaggaccgc atcacgcgct cgcagcgcgc cggcagtgag acatcgtcgc cggccgtgac
     1981 gaacagcatc tcgccgcgcg agagttcgac gagccggctc aggtgggcgc cgatgccgag
     2041 gttgaccgga ttgcggttca ggcggaccgt gtgcgggccg gcgtagcctg cgacggcac
//
LOCUS       DASRDG010000250         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1190219, whole genome shotgun sequence.
ACCESSION   DASRDG010000250 DASRDG010000000
VERSION     DASRDG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190219"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFA35_04145"
     CDS             <1..382
                     /locus_tag="VFA35_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZT55395.1"
                     /translation="PRVFVLDTRDSTIWVDGSISLATEALELRVVTTPKDFSPLALRT
                     PVLVRGTFANPRVSVDKGKLGTRLGAAALLALVNPLAALIPLMDVGDSADAQRGADAC
                     RALSQRIKAKPLLPAPGPGRRKPR"
     gene            493..1044
                     /locus_tag="VFA35_04150"
     CDS             493..1044
                     /locus_tag="VFA35_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55396.1"
                     /translation="MITLRHCKTGLAIAALCVAGTAFGATGNKTDYNAAKDRIAAEYK
                     ADKAACDAFSGNKKDICVEQAKGKEKVAKAEAEYNYTGKPDDQNRIAVEKADAEYAVA
                     KEMCDDKAGHDKDVCTTDAKSAHTKAIADAKMNKKVGEARKDAAEDKRDADYKAAVEK
                     CDALAGDAKSACLGAAKARFGKG"
     gene            1114..1749
                     /locus_tag="VFA35_04155"
     CDS             1114..1749
                     /locus_tag="VFA35_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55397.1"
                     /translation="MRSSTPCACAQFALLALGLAGAGAACAQGDDGDEAKDGIALALV
                     RETVPGAGANDAPRLIRHEAPVEPQRALPGYRHETTELGYRWWLSRGRADLGLGVGTM
                     SYGVRPIGPAPAPGSDAASGVLAVGTVVTVGMRYRTSDSSTLYADAAGVRGPAFDGGE
                     AVVGKVGVEFKAAQSRFDIAYGGLGLRLAGDTRMTVRVKRGALGVVMRRRF"
     gene            complement(1825..>2089)
                     /locus_tag="VFA35_04160"
     CDS             complement(1825..>2089)
                     /locus_tag="VFA35_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZT55398.1"
                     /translation="AHGTLGSALAMAAFIVLLSTLVGTVVTAWLQGRAAKMNAAAVFI
                     GVVFWGALWGVWGLLLGPALVVLLKVVAEHTRSGRRLAHLMRG"
BASE COUNT          351 a          746 c          736 g          256 t
ORIGIN      
        1 gccgcgcgtg ttcgtgctcg acacgcgcga ttcgacgatc tgggtcgacg gctcgatctc
       61 gcttgcgacc gaagcgctcg agctgcgcgt cgtcacgacg ccgaaggact tcagcccgct
      121 cgcgctgcgc acgccggtgc tcgtgcgcgg cacgttcgcg aacccgcgcg tgtcggtcga
      181 caagggcaag ctcggcacgc ggctgggcgc cgcggcgctg ctcgcgctcg tcaatccgct
      241 cgccgcgctc atcccgctga tggacgtggg cgacagcgcc gacgcgcagc gcggcgccga
      301 tgcgtgccgg gcgctgtcgc agcgcatcaa ggccaagccg ctgttgccgg cacccggccc
      361 ggggcggcgc aagccccggt aggagaagaa ctacgccctg ctgcggcggc gtccgctggg
      421 gcaggttcgg gtcgcttcgc atacttcgga caccgcggaa atcgaaccgc gcactcgcaa
      481 aggacgaccg ccatgatcac gctccgacac tgcaagaccg gcctggccat tgccgccctg
      541 tgcgtggccg gcacggcgtt cggcgccacc ggcaacaaga ccgactacaa cgccgcgaag
      601 gaccggatcg ccgccgagta caaggccgac aaggccgcgt gcgatgcgtt cagcggcaac
      661 aagaaggaca tctgcgtcga gcaggccaag ggcaaggaaa aagtcgccaa ggccgaggcc
      721 gagtacaact acaccggcaa gcccgacgac cagaaccgga tcgccgtcga gaaggccgac
      781 gccgagtacg ccgtcgcgaa ggagatgtgc gacgacaagg ccggccacga caaggacgtg
      841 tgcacgaccg acgccaagtc ggcccacacc aaggcgatcg ccgatgccaa gatgaacaag
      901 aaggtcggcg aggcccgcaa ggacgccgcc gaagacaagc gcgacgccga ctacaaggcc
      961 gcggtcgaga agtgcgacgc actcgccggc gatgcgaagt cggcctgcct gggcgccgcc
     1021 aaggcgaggt tcggcaaggg ttgagcgaat ccccggagcg aacgaactcc gggcgtgcga
     1081 ccgagtcgaa cccggctcgg aaaggatgtc gcaatgcgct cttcgacgcc ttgtgcttgc
     1141 gcgcagttcg cgctgctggc cctcggcctg gccggggcag gtgcggcgtg tgcccaaggc
     1201 gacgatggcg atgaagccaa ggatggcatc gcgctggcgc tcgtgcgcga aaccgtaccc
     1261 ggtgctggcg cgaacgacgc gccgcgcctg atccgtcacg aagcgcccgt cgaaccgcag
     1321 cgtgcgctgc ccggttaccg ccacgagacg accgagctcg gctaccgatg gtggttgtca
     1381 cggggccgcg ccgacctcgg cctcggcgtg ggcacgatga gctacggcgt gcgcccgatc
     1441 ggccctgcgc cggcgccggg cagcgacgcc gcgtccggcg tgctcgcggt gggcaccgtg
     1501 gtgacggtgg gcatgcgcta tcgcacctcg gacagctcga ccctctatgc cgacgccgcc
     1561 ggcgtgcgcg ggcccgcctt cgacggcggc gaagcggtcg tcggcaaggt cggggtcgag
     1621 ttcaaggcgg cccagtcgcg cttcgacatc gcctacggcg gcctcggcct gcggctggcc
     1681 ggcgacaccc gcatgaccgt gcgggtcaag cgcggcgccc tcggcgtcgt catgcgccgc
     1741 cggttctgac cgtgccggcc acttgctccc tcttccgctt gcgggagagg gccgcggtga
     1801 gggtgctcgt gcgtcgacgg agcctcaccc ccgcatcagg tgcgcgaggc gtcggccgga
     1861 ccgcgtgtgt tccgccacca ccttgagcag caccaccagc gccgggccga gcagcaggcc
     1921 ccacacgccc cacagcgcgc cccagaacac cacgccgatg aacacggcgg cggcattcat
     1981 cttcgccgcg cggccctgca gccacgcggt gacgacggtg ccgaccagcg tcgagagcag
     2041 cacgatgaac gccgccatcg ccagggccga gccgagcgtg ccgtgcgca
//
LOCUS       DASRDG010000251         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1549432, whole genome shotgun sequence.
ACCESSION   DASRDG010000251 DASRDG010000000
VERSION     DASRDG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549432"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..731)
                     /locus_tag="VFA35_04165"
                     /pseudo
     CDS             complement(327..731)
                     /locus_tag="VFA35_04165"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
     gene            complement(728..1234)
                     /locus_tag="VFA35_04170"
     CDS             complement(728..1234)
                     /locus_tag="VFA35_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HZT55399.1"
                     /translation="MNQPNRPALEVAVVIEREASPNRWEDWRFRIVEVLPDEPAFGRT
                     PRRLHDDGRFAHWLHPGFRVELFTDDAKGYYLNLSTGTPVWFVMWRIDQDDPSIASPQ
                     GVSLSYNDAARWMDNAEERVDSLPLPPDVREWLQAYVDVNYRPVVEPRRKPQSFLNPE
                     ERARGARK"
     gene            complement(1257..2351)
                     /gene="apbC"
                     /locus_tag="VFA35_04175"
     CDS             complement(1257..2351)
                     /gene="apbC"
                     /locus_tag="VFA35_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HZT55400.1"
                     /translation="MPALTDAVVLDALKTVIDPNTGTDFVSTRQLKNLRIEGGDVAFD
                     VELGYPAKSQIDALRKALIAAARTASGVRNVSANLTVKITAHAVQRGVQLLPNVKNIV
                     AVASGKGGVGKSTTAVNLALALAAEGASVGILDADIYGPSQPMMMGITGRPESADGKT
                     MAPMENYGVQVMSIGFLVEQDNPMIWRGPMATQALEQLLRQTNWRELDYLIVDMPPGT
                     GDIQLTLSQRVPLTGAVIVTTPQDIALLDAKKGIKMFEKVGVPILGIVENMAVHVCEK
                     CGHVEHIFGEEGGKRLAAEYKMDYLGALPLNLSVREQADAGRPTVVSDPEGEIAATYK
                     AVARKVAVTIAEKAKDYSAKFPTISISKTT"
BASE COUNT          335 a          839 c          886 g          440 t
ORIGIN      
        1 ctgcttcgcg gcgacagagc gtgctgtgga cggccgacag cccctcagcc ttctcggggt
       61 cgagcgcctt cgccagcgcc ggagcgtcca gcggcatcgt ccggttgcag tcgcagatca
      121 gcgtcttcat gtggggtgga attatcggtt ggcggcagca ccggctgctc gaccatggga
      181 ttggacgcct cggcggtcgt ctcggcgggc gcagcgggcg cgtccggggt ctgggcgctc
      241 tgctcctgct cttcctcgtg gtcgaacagg ccgaggaact tggattgcac gagctggcgc
      301 agcatcgcgg gtggaatcgg gtcgggctgg ctgtagtcgt cgatgtagat gtccagccgg
      361 tccatcacgt tgaaatgcgg atcggtgaac agcttgcgga acgccgtgtt cttcacgtgc
      421 ggatcgacgc cgggcgcgat gaagcgcgag atctcgtcgc cgggaacgat ggcgtcggcc
      481 tcggcctggg tcggcggcgg cggacgctcc tcgaccggcg ccgagacgac cgcgtggtcc
      541 tgttcgacgg ccagcggcgc ggcactcgtc gtcggcagcg tcgcggcggc cggggccggt
      601 ggctcggcgg gcgccggcgc gcgcggcggt tcgggtgcga cgcggccctg gcgctgctcg
      661 ttcttgcggc gcgcccagcg cgccaggaag ccgccgcctt cgtgcgcgtc gcgctcgtcc
      721 atcgccgtca tttccgagcg cctctggcgc gctcttcggg gttgaggaaa gactgcggct
      781 tgcggcgcgg ctccacgacc gggcggtagt tgacgtcgac gtaggcctgc agccactcgc
      841 gcacgtcggg cggcagcggc aggctgtcga cgcgctcctc ggcgttgtcc atccagcgcg
      901 cggcgtcgtt gtacgacagg ctcacaccct gcgggctcgc gatcgacgga tcgtcctggt
      961 cgatgcgcca catcacgaac cacaccggcg tgccggtgct gaggttgagg taatagccct
     1021 tggcgtcgtc ggtgaacagc tcgacccgaa aaccgggatg cagccagtgc gcaaagcggc
     1081 cgtcgtcgtg caggcggcgc ggtgtgcggc cgaacgccgg ctcgtcgggc agcacctcga
     1141 cgatgcgaaa acgccagtcc tcccagcggt tcgggctggc ctcgcgctcg atgaccaccg
     1201 cgacctcgag cgccgggcgg ttcggctggt tcatggtgag cctcggcctg cgtccatcag
     1261 gtggtcttgg agatgctgat cgtcgggaac ttggccgagt agtccttggc cttctcggcg
     1321 atcgtcaccg ccaccttgcg cgccacggcc ttgtaggtcg cggcgatctc gccttcggga
     1381 tcgctcacca ccgtgggccg gccggcgtcg gcctgctcgc gcaccgacag gttcagcggc
     1441 agcgcgccca gatagtccat cttgtactcg gccgccaggc gcttgccgcc ctcctcgccg
     1501 aagatgtgct cgacatggcc gcacttctcg cacacgtgaa ccgccatgtt ctcgacgatg
     1561 cccaggatcg gcacgccgac cttctcgaac atcttgatgc ccttcttcgc gtcgagcagc
     1621 gcgatgtcct gcggggtcgt gacgatcacc gcgccggtca gcggcacgcg ctggctcaag
     1681 gtgagctgga tgtcgccggt gcccggcggc atgtcgacga tcaggtaatc gagctcgcgc
     1741 cagttggtct ggcgcagcaa ctgctcgagc gcctgcgtgg ccatcgggcc gcgccagatc
     1801 atcgggttgt cctgctcgac cagaaaaccg atcgacatga cctgcacgcc gtagttctcc
     1861 atcggcgcca tcgtcttgcc gtcggcactt tccggccggc cggtgatgcc catcatcatc
     1921 ggctggctcg ggccgtagat gtcggcgtcg aggatgccca ccgacgcgcc ttcggccgcg
     1981 agcgcgagcg cgaggttgac cgcggtcgtg ctcttgccga cgccgccctt gcccgaagcg
     2041 accgcgacga tgttcttgac gttcggcagc agttgcacgc cgcgctgcac cgcgtgcgcg
     2101 gtgatcttga ccgtgaggtt cgcgctgacg ttgcgcaccc cggaggcggt gcgcgccgcc
     2161 gcgatcagcg ccttgcgcag cgcgtcgatc tggctcttcg ccgggtagcc gagctcgacg
     2221 tcgaaggcca cgtcgccccc ctcgatgcgc aggttcttca gctgacgcgt gctgacgaag
     2281 tcggtgccgg tgttcgggtc gatgacggtc ttgagcgcgt cgaggacgac ggcgtcggtg
     2341 agagcgggca tgggtggctt ctgcaggggt gagcgcgcaa gtctagcggc acgccgagac
     2401 ggctcgtcgg ggggccgcgc aaaagcgcgc aagaatgaag gccatgcaaa gacgactcct
     2461 cctccgcacc gccgccgctc tgggcaccgg cagcctgctg
//
LOCUS       DASRDG010000252         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_269098, whole genome shotgun sequence.
ACCESSION   DASRDG010000252 DASRDG010000000
VERSION     DASRDG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269098"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /gene="kdsA"
                     /locus_tag="VFA35_04180"
     CDS             complement(<1..783)
                     /gene="kdsA"
                     /locus_tag="VFA35_04180"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZT55401.1"
                     /translation="MKLCGFDVGITRPFFLIAGPCVIESEQLQMDVAGQLKEMSSALG
                     IPFIFKSSFDKANRSSGGTFRGPGMDKGLEILAKVKRELGVPVLTDIHDESQIEQVAR
                     VVDVLQTPAFLCRQTDFIRAVAQSGKPVNVKKGQFLAPHDMKNVVDKARAAAREKGLN
                     EDNFMACERGASFGYNNLVSDMRSLAIMRETDAPVVFDATHSVQLPGGHGTSSGGQRE
                     FVPVLARAAVAAGVAGVFMETHPNPAKALSDGPNAWPLARMRG"
     gene            complement(806..>2126)
                     /locus_tag="VFA35_04185"
     CDS             complement(806..>2126)
                     /locus_tag="VFA35_04185"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZT55402.1"
                     /translation="HITDEIKERVRSLAEAEQADLLVVEIGGTVGDIESLPFIEALRQ
                     LRLEVGAQNAISVHLTLVPYIAAAGELKTKPTQHTVQKMREIGIQPDALLCRADRKIP
                     DDERDKISLFTNVGLQGVISMWDVDTIYKVPRMLHEQRLDEIICMKLQLLTKPADLKR
                     WDKLVYELAHPQHEVTIAMCGKYTDLSDSYKSLNEALRHAGIHNHARVTIEYVDSETL
                     GDDNIDQLAKYDAILVPGGFGKRGVEGKIRTAQYAREHKIPYLGICLGMQVATIEVAR
                     HLAGLDGANSTEFEPDTKHPVIALIDEWQDSDGTIQKRDANSDLGGTMRLGAQSSDVA
                     PGTLAHQIYGKVVTERHRHRYEANERYLERLQKAGLLISAITQREKLTEIIELPKEQH
                     PWFVGVQFHPEFKSTPWDGHPLFTSFVKAALDHQARRQAEPVEKVAA"
BASE COUNT          296 a          707 c          719 g          404 t
ORIGIN      
        1 cccgcgcatg cgcgcgagcg gccaggcgtt gggtccgtcc gacagcgcct tggcagggtt
       61 cggatgcgtc tccatgaaca cgccggccac gcccgcggcg accgccgcgc gcgccagcac
      121 cggcacgaac tcgcgctggc cgcccgaact cgtgccgtgg ccgccgggca actgcactga
      181 atgggtcgcg tcgaacacga ccggcgcgtc ggtctcgcgc atgatcgcca ggctgcgcat
      241 gtcggagacg aggttgttgt agccgaagct cgcgccgcgt tcgcaggcca tgaagttgtc
      301 ttcgttcagg cccttctcgc gcgccgcggc acgggccttg tcgaccacat tcttcatgtc
      361 gtggggcgcg agaaactggc ccttcttgac gttcacgggc ttgccgcttt gcgccaccgc
      421 gcggatgaaa tcggtctggc ggcacaggaa ggcgggcgtc tgcagcacgt cgaccacgcg
      481 cgcgacctgc tcgatctgcg actcgtcgtg gatgtcggtc agcaccggca ccccgagctc
      541 gcgcttgacc ttggcaagga tctcgaggcc cttgtccatg cccgggccgc gaaaggtgcc
      601 gcccgacgaa cggttcgcct tgtcgaaact gctcttgaag atgaacggga tgccgagcgc
      661 cgagctcatc tccttcagct ggccggccac gtccatctgc aactgctcgg actcgatcac
      721 gcacggcccg gcgatcagga agaacggccg cgtgatgccg acatcgaagc cgcagagctt
      781 cattggagcc ccctcggcgc ggggctcatg ccgcgacctt ctcgaccggc tcggcctggc
      841 ggcgcgcctg atgatccagc gccgccttga cgaagctggt gaacagcggg tggccgtccc
      901 agggcgtgct cttgaactcg gggtggaact gcacgccgac gaaccatggg tgctgctctt
      961 tcggcagctc gatgatctcg gtcagcttct cgcgttgcgt gatcgccgag atcagcagcc
     1021 cggccttctg gaggcgctcg aggtagcgct cgttcgcctc gtagcggtgg cggtggcgct
     1081 cggtcacgac cttgccgtag atctggtgtg cgagcgtgcc gggcgcgaca tcggagctct
     1141 gcgcacccag gcgcatcgtg ccgcccaggt cggagttcgc gtcgcgcttc tggatcgtgc
     1201 cgtcgctgtc ctgccactcg tcgatcaagg cgatcaccgg gtgcttcgtg tcgggctcga
     1261 actcggtgct gttcgcgccg tccaggcccg ccagatggcg cgcgacctcg atcgtcgcga
     1321 cctgcatgcc caggcagatg cccaggtacg ggatcttgtg ttcgcgcgcg tattgcgcgg
     1381 tgcggatttt gccctcgacg ccgcgcttgc cgaagccgcc gggcaccagg atcgcgtcgt
     1441 acttggcgag ctggtcgatg ttgtcgtcgc cgagtgtctc ggagtcgacg tactcgatcg
     1501 tcacgcgcgc atggttgtgg atgccggcgt ggcgcagtgc ttcgttcagc gacttgtacg
     1561 aatcggacag gtcggtgtac ttgccgcaca tcgcgatcgt cacctcgtgc tgcgggtgcg
     1621 cgagctcgta gacgagcttg tcccagcgct tcaggtcggc cggcttcgtg agcagctgca
     1681 gcttcatgca gatgatctcg tcgagccgct gctcgtgcag catgcgcggc accttgtaga
     1741 tcgtgtcgac gtcccacatc gagatcacgc cctgcaggcc gacgttggtg aacagcgaga
     1801 tcttgtcgcg ctcgtcgtcg gggatcttgc ggtcggcgcg gcacagcagc gcgtcgggct
     1861 ggatgccgat ctcgcgcatc ttctgcacgg tgtgctgcgt gggcttggtc ttgagctcgc
     1921 cggcggccgc gatgtagggg accagcgtca ggtgcacgct gatggcgttc tgcgcgccca
     1981 cctcgagacg cagctggcgc agcgcctcga tgaacggcag cgactcgatg tcgccgacgg
     2041 tgccgccgat ctcgacgacc agcaggtcgg cctgctccgc ctcggccagc gaccgcaccc
     2101 gctccttgat ctcgtcggtg atgtgt
//
LOCUS       DASRDG010000253         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_842508, whole genome shotgun sequence.
ACCESSION   DASRDG010000253 DASRDG010000000
VERSION     DASRDG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842508"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..790
                     /locus_tag="VFA35_04190"
     CDS             62..790
                     /locus_tag="VFA35_04190"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZT55403.1"
                     /translation="MPAPRFFVDQPLQPGAAFSLPAGAARHVQVLRLQPGAAITLFDG
                     TGGEWAASVVQMGRSEVSVQLGVHAEVERELPLPVTLALGMPANERMDTVIEKATELG
                     AVAIQPLLCERSVLRLSGERAQKKVSHWKAVALAASEQSGRTRVPQVAPVQSLGEWLR
                     ALPRDDRSRWMLSFDAAQPLASLVPSRAGVVSLSGPEGGLSPAEEALARAAGFAPVSL
                     GARVLRADTAPLALLAHLAIGASG"
     gene            844..1305
                     /locus_tag="VFA35_04195"
     CDS             844..1305
                     /locus_tag="VFA35_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HZT55404.1"
                     /translation="MSLLDSVIGALANSQPGVQPPASGATQGTPGSVLLSTVISMLAN
                     GQHPQGAGVGGLGDLIGRFTQGGLGDVIGSWIGNGQNAPISGDQLSKVLGSDAIGQIA
                     AQLGVSQGDAAGQLSQMLPEVISRLTPQGAAPSGGFGDDMGALLGQLMAKR"
     gene            complement(1302..2528)
                     /locus_tag="VFA35_04200"
     CDS             complement(1302..2528)
                     /locus_tag="VFA35_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HZT55405.1"
                     /translation="MPLPLFLPCAAGVEALLAEEVQRVLPKARVFEARGGVALEGDPH
                     EVMVLNLESRLAQRVLIEVAHGSYRVEDDIYELARSVDWTQWITPQHTLRVDTTAHRS
                     PLKSLNFAALRVKDAVCDQLREATGERPSVDTRQPDLQIVLHLGELEATLYVDSSGES
                     LFKRGWREDKGDAPLKETLAAAMLAAAGWRGTPEAGGALHDPCCGSGTIAIEAAQIAC
                     GIAPGLKRRFAFERLLPFVGEAQRAELQRLRSHAQARIHPSAVPIYASDVSFRMVDFA
                     RRNAERAGVAGAITFNGGDALERPAPELPTDLPGTLMINPPYGERIEVGGKAARESAQ
                     DRDTPSDFFPRLSAHWKRAYTQHPAGWTAYVLSPDMKLPGAMRLKESRRVPMWNGPIE
                     CRLFRFDLVAGSARKV"
     gene            complement(2528..>3022)
                     /locus_tag="VFA35_04205"
     CDS             complement(2528..>3022)
                     /locus_tag="VFA35_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZT55406.1"
                     /translation="NGAAAGLNSRLQEARWFVKNNLQRFDTIQRVSQAIVERQKSFFT
                     HGAIAMKPLVLREIADELGLHESTISRVTTAKYMATPFGTFELKYFFGSSLGTDAGGT
                     ASSTAVRALIRQIVAAEDPAKPLSDSQISQMLDEQGIHVARRTVAKYREALKIAPTQL
                     RKAM"
BASE COUNT          392 a         1052 c         1119 g          459 t
ORIGIN      
        1 ccgcggcggc acgctggcgg cggcggctgg ctgccctggt tcgctgaggc acactcgccg
       61 catgcctgcg ccgcgcttct tcgtcgacca gccgctgcag cccggcgccg cgttcagctt
      121 gccggccggt gccgcgcgcc acgtgcaggt gctgcgcctg cagcccggtg ccgcgatcac
      181 cttgttcgac ggcacgggcg gcgagtgggc ggcgagcgtc gtgcagatgg ggcgcagcga
      241 ggtcagcgtg cagctcggcg tgcatgccga ggtcgagcgc gaactgccgc tgccggtcac
      301 gctcgcgctc ggcatgccgg ccaacgagcg catggacacg gtgatcgaga aggcgaccga
      361 gctcggcgcc gtcgcgatcc agccgctgct gtgcgagcgt tcggtgttgc gcctgagtgg
      421 cgagcgcgca cagaagaagg tttcgcactg gaaggcggtg gcgctcgcgg cgagcgagca
      481 gagcgggcgc acgcgcgtgc cgcaggtggc gccggtgcaa tcgttgggcg agtggttgcg
      541 cgcgctgccg cgcgacgacc gctcgcgctg gatgctgagt ttcgacgcgg cgcagccgct
      601 cgcgtcgctc gtgccatcgc gcgccggtgt cgtgtcgttg agcggccccg aaggcggcct
      661 gtcgcccgcg gaggaagcgc tcgcgcgcgc cgccggcttc gcgccggtct cgctcggcgc
      721 gcgcgtgctg cgcgccgaca ccgcgccgct cgcgttgctc gcgcacctgg cgatcggcgc
      781 gtccggctga ccccacgttg cgcggcgatg ccagaatggg cctccaacct gaggagttcc
      841 gcgatgagcc tgcttgattc ggtgatcggt gcgctggcca acagccagcc cggtgtgcaa
      901 ccgcccgcgt ccggtgcgac gcagggcacg cccggctcgg tgctgctgag cacggtgatt
      961 tcgatgctcg ccaacgggca gcatccgcag ggcgcggggg tcggcggcct gggcgacctg
     1021 atcggccgct tcacgcaggg cggcctgggc gacgtgatcg gctcgtggat cggcaacggc
     1081 cagaacgcgc cgatctcggg cgaccagctc tccaaggtgc tcggctcgga tgcgatcggc
     1141 cagatcgccg cgcagctcgg cgtgtcgcag ggcgacgccg cgggtcagct ctcgcagatg
     1201 ctgccggaag tgatcagccg cctgacgccg cagggcgcgg cgccgagcgg cggttttggc
     1261 gacgacatgg gcgcgctgct cggccagctg atggcgaagc gctagacctt gcgggccgag
     1321 cccgcgacaa gatcgaagcg gaacagccgg cactcgatcg gcccgttcca catcggtacg
     1381 cggcgcgatt ccttcagccg catcgcgccc ggcagcttca tgtcggggct cagcacgtag
     1441 gcggtccagc cggccgggtg ctgggtgtag gcgcgcttcc agtgcgcgga caggcgcggg
     1501 aagaagtcgc tcggtgtgtc gcgatcctgc gcggattcac gcgccgcctt gccgccgacc
     1561 tcgatgcgct cgccatacgg cgggttgatc atcaaggtgc cgggcaggtc ggtcggcagc
     1621 tcgggcgccg ggcgctccag cgcgtcgccg ccgttgaagg tgatcgcgcc tgccacgccc
     1681 gcgcgctcgg cgttgcgccg cgcgaagtcg accatgcgga acgacacgtc gctggcgtag
     1741 atcggcaccg cactcgggtg gatgcgcgcc tgtgcatggc tgcgcaaccg ctgcaactcg
     1801 gcgcgctgtg cctcgccgac gaacggcagc aagcgctcga acgcgaagcg ccgcttcagc
     1861 cccggcgcga tgccgcaggc gatctgcgcg gcctcgatcg cgatcgtgcc cgagccgcag
     1921 cacgggtcgt gcagcgcgcc gccggcctcg ggcgtgccgc gccagccggc ggcggcgagc
     1981 atcgcggcgg ccaacgtctc cttcagcggc gcgtcgccct tgtcttcgcg ccagccgcgc
     2041 ttgaacagcg actcgcccga gctgtcgaca tagagcgtcg cctcgagctc gccgaggtgc
     2101 agcacgatct gcaggtcggg ctggcgcgtg tcgacgctcg ggcgctcgcc ggtcgcctcg
     2161 cgcagctggt cgcagaccgc gtccttcacg cgcagcgcgg cgaagttcag gctcttgagc
     2221 gggctgcggt gcgcggtggt gtcgacacgc agtgtgtgct gcggcgtgat ccactgcgtc
     2281 cagtcgacgc tgcgcgcgag ctcgtagatg tcgtcctcga cgcggtacga gccgtgcgcg
     2341 acctcgatca gcacgcgctg cgcgagccgg ctctcgaggt tcagcaccat cacctcgtgc
     2401 gggtcgcctt cgagcgcgac gccgccgcgc gcctcgaaca cgcgcgcctt cggcagcacg
     2461 cgctgcacct cctcggcgag caaggcttcg acgcccgcgg cgcagggcag aaacaacggc
     2521 aacggcatca catcgccttg cgcaactgcg tcggcgcgat cttcagcgcc tcgcggtact
     2581 tggcgaccgt gcgccgggcg acgtggatgc cttgctcgtc gagcatctgc gagatctggc
     2641 tgtcggagag cggcttggcc ggatcctcgg cggcgacgat ctggcggatc agcgcccgca
     2701 ccgcggtgct cgacgcggtg ccgccggcgt cggtgccgag cgacgagccg aagaagtact
     2761 tgagctcgaa ggtgccgaac ggcgtcgcca tgtacttggc cgtcgtcacg cgcgagatcg
     2821 tcgattcgtg caggccgagc tcgtcggcga tctcgcgcag gacgagcggc ttcatcgcga
     2881 tcgcgccgtg cgtgaagaag ctcttttgcc gctcgacgat cgcctgcgag acgcgctgga
     2941 tggtgtcgaa gcgctgcagg ttgttcttga cgaaccagcg cgcctcctgc agccgcgagt
     3001 tgaggcccgc ggcggcgccg tt
//
LOCUS       DASRDG010000254         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1421094, whole genome shotgun sequence.
ACCESSION   DASRDG010000254 DASRDG010000000
VERSION     DASRDG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421094"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="VFA35_04210"
     CDS             <1..156
                     /locus_tag="VFA35_04210"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZT55407.1"
                     /translation="GLRASMHYCGCASIADMHEKAEFVEISSAGMRESHVHDVQITKE
                     APNYRME"
     gene            195..1421
                     /locus_tag="VFA35_04215"
     CDS             195..1421
                     /locus_tag="VFA35_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT55408.1"
                     /translation="MPFIMATVLIDMISIGLIIPVLAPLIGTFTASQSEQAFWYGVIT
                     IVFGIANFFGSPILGRLSDRYGRRPVLLLGFSGLALSFFVTGMATALWMLVVVRLFSG
                     AMQSNVAVANAYVADITPPEQRAKAFGKLGAMLGLGFILGPVIGGLLGGISLHLPFFV
                     AGGLACVNWLYGYFVLPESLPVERRQPFVLREANPVNSLRGLTALKGVGSLVVVIALS
                     GLAQFMTHTTWVLYTTFKFGWGPTQNGWSLFAIGVMSVLVQGVLLGKLLKRFAPQRLA
                     IMGLVSSSLCFVLWGVATEGWMLYAVIFVNVLGFAVSASIQSLISNAADPSTQGRTAG
                     AVGSLNSMMAVVAPAIGAPLLATVSHLPHGDWRIGAPFYFCALLQAASAVIALRHFKR
                     ARLKRETRAAGALPTT"
     gene            1441..3003
                     /gene="guaA"
                     /locus_tag="VFA35_04220"
     CDS             1441..3003
                     /gene="guaA"
                     /locus_tag="VFA35_04220"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZT55409.1"
                     /translation="MHDKILILDFGSQVTQLIARRVREAHVFCEIHPNDVSDEFIRAF
                     APKGVILSGSHASTYEEHELRAPQAVWDLGVPVLGICYGMFTMAVQQGGQVEASDHRE
                     FGYAEVRAHGHTKLLQGIEDFSTAEGHGMLKVWMSHGDKVTALPPGFKLMASTPSCPI
                     AGMADESRGYYGVQFHPEVTHTEKGRELLNRFVLEIAKARPDWVMGDYIEEAVARIRE
                     QVGSEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGAMVMEMFAGRL
                     HAKVIAVDASEQFLGHLAGVTDPEAKRKIIGREFVEVFQREAKELPNARWLAQGTIYP
                     DVIESGGAKSKKATSIKSHHNVGGLPETLGLKLLEPLRELFKDEVRELGLALGLPREM
                     VFRHPFPGPGLGVRILGEIKPEYVQLLQRADDIFIQELRSSGWYDKTGQAFAVFLPVR
                     SVGVMGDGRTYEFVVALRAVQTTDFMTAHWAELPYELLGRASNRIINEVRGINRVTYD
                     ISSKPPATIEWE"
BASE COUNT          494 a         1021 c         1074 g          542 t
ORIGIN      
        1 ggcctgcgcg cctcgatgca ctactgcggc tgcgccagca tcgccgacat gcacgagaag
       61 gccgagttcg tcgagatttc gtcggccggc atgcgcgaga gccatgtgca cgacgtgcag
      121 atcaccaaag aggcgccgaa ctaccgcatg gaatgatgga gtcgatcgcg cagccggcgg
      181 gccgcaaagc ggccatgccg ttcatcatgg ccacggtctt gatcgacatg atctcgatcg
      241 gcctgatcat cccggtgctc gcgccgctga tcggcacctt cacggcctcg cagtcggagc
      301 aggcgttctg gtacggcgtg atcacgatcg tgttcgggat cgcgaacttc ttcggctcgc
      361 cgatcctcgg ccgcctgtcc gaccgctacg gccgccggcc ggtgttgctg ctcggcttca
      421 gcggcctcgc gctgagcttc ttcgtcaccg gcatggcgac ggcgctgtgg atgctcgtcg
      481 tcgtgcgcct gttcagcggc gcgatgcagt cgaacgtcgc ggtcgcgaac gcctacgtcg
      541 ccgacatcac cccgccggag cagcgcgcca aggccttcgg caagctcggc gcgatgctgg
      601 gcttgggctt catcctcggg ccggtgatcg gcggcctgct cggcggcatc agcctgcacc
      661 tgccgttctt cgtcgcgggc ggcctggcct gcgtgaactg gctctacggc tacttcgtgc
      721 tgcccgagtc gctgccggtc gaacgccgcc agcccttcgt gctgcgcgag gcgaacccgg
      781 tgaactcgct gcgcggcctg accgcgctga agggcgtcgg ctcgctcgtc gtcgtgatcg
      841 cgttgagcgg cctggcgcag ttcatgaccc acaccacctg ggtgctctac acgaccttca
      901 agttcggctg gggcccgacc cagaacggct ggtcgctgtt cgcgatcggc gtgatgtcgg
      961 tgctggtcca gggcgtgctg ctgggcaagc tgctgaagcg cttcgcgccg cagcggctgg
     1021 cgatcatggg gctggtctcg tcgagcctgt gcttcgtgct ctggggcgtc gcgaccgagg
     1081 gctggatgct gtatgcggtg atcttcgtca acgtgctcgg ctttgcggtc tcggcgtcga
     1141 tccagagcct gatctcgaac gccgccgatc cctcgacgca aggccgcacc gccggcgcgg
     1201 tgggctcgct gaacagcatg atggcggtgg tggcgcccgc gatcggcgcg ccgctgctcg
     1261 cgaccgtctc gcacctgccg cacggcgact ggcgcatcgg cgccccgttc tacttctgcg
     1321 cgctgctgca ggccgcctcg gcggtgatcg cgctgcgcca cttcaaacga gcccgcttga
     1381 agcgcgaaac gcgcgccgcc ggcgccttgc cgacgacctg acactcatct tcccatcgcc
     1441 atgcacgaca agatcctgat cctcgacttc ggctcccagg tcacgcagtt gattgcgcgc
     1501 cgcgtgcgcg aggcgcacgt gttctgcgag atccacccga acgacgtgag cgacgaattc
     1561 atccgcgcgt tcgcgccgaa gggcgtgatc ctgtcgggca gccacgcgtc gacctacgag
     1621 gagcacgaac tgcgcgcgcc gcaggcggtg tgggatctgg gcgtgccggt gctcggcatc
     1681 tgctacggca tgttcacgat ggcggtgcag cagggcggcc aggtcgaggc cagcgaccac
     1741 cgcgagttcg gctacgccga ggtgcgcgcg cacggccaca ccaaactgct gcagggcatc
     1801 gaggacttca gcacggcgga aggccacggc atgctgaagg tctggatgag ccacggcgac
     1861 aaggtcaccg cgctgccgcc gggcttcaag ctgatggcga gcacgccgag ttgcccgatc
     1921 gccggcatgg ccgacgaatc gcgtggctac tacggcgtgc agttccaccc cgaggtcacg
     1981 cacaccgaga agggccgcga gctgctgaac cgcttcgtgc tcgagatcgc gaaggcccgg
     2041 cccgactggg tgatgggcga ctacatcgaa gaggcggttg cgcgcatccg cgagcaggtc
     2101 ggcagcgagg aggtgatcct cggtctctcg ggcggcgtcg attcgagcgt ggccgcggcg
     2161 ctgatccacc gcgcgatcgg cgaccagctg acctgcgtgt tcgtcgacca cggcctgttg
     2221 cgcctgaacg agggcgcgat ggtgatggag atgttcgcgg ggcgcctgca cgcgaaggtc
     2281 attgcggtcg acgcgagcga gcagtttctc ggtcacctgg ccggggtgac cgaccccgag
     2341 gccaagcgca agatcatcgg gcgcgagttc gtcgaggtgt tccagcgcga ggcgaaagaa
     2401 ctgccgaatg ccagatggct cgcgcaaggc acgatctacc cggacgtgat cgagagcggc
     2461 ggcgcgaaga gcaagaaggc cacgtcgatc aagagccacc acaacgtcgg cggcctgccc
     2521 gagacgctgg ggctgaagct gctcgagccg ctgcgcgagc tgttcaagga cgaggtgcgc
     2581 gagctcggcc tcgcgctcgg cttaccgagg gagatggttt tccggcatcc attccctggc
     2641 cccggcttgg gcgtcagaat tctcggggaa ataaagcccg aatatgttca gttgctgcag
     2701 cgagctgacg atatcttcat ccaggaactg agaagctccg gctggtacga caagaccggc
     2761 caggcgttcg ccgtcttcct gccggtgcgc tccgtgggcg tgatgggcga cgggcgcacg
     2821 tacgagttcg tggtggcgct gcgcgccgtg caaaccacgg acttcatgac ggcgcactgg
     2881 gcggagctgc cctacgagtt gctggggcgg gcgtcgaatc gcatcatcaa cgaggtgcgc
     2941 ggcatcaacc gcgtgaccta cgacatctcc tcgaagccgc ccgccaccat cgagtgggaa
     3001 taacgcatgg ttaccgcctg acgaatccgg ggtagaggta aggatttgtt agtcggtggc
     3061 tttggcaccc cggatcggct aggctctgcg ggtcccatca gaacaaaagt ggcatgcgcc
     3121 gcttcttcgc c
//
LOCUS       DASRDG010000255         3963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1643873, whole genome shotgun sequence.
ACCESSION   DASRDG010000255 DASRDG010000000
VERSION     DASRDG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643873"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..637
                     /gene="rpiA"
                     /locus_tag="VFA35_04225"
     CDS             2..637
                     /gene="rpiA"
                     /locus_tag="VFA35_04225"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HZT55410.1"
                     /translation="MVGQAAIAYVEPGTVIGVGTGSTVNCFIDALAQMPVRIAAAVSS
                     SQQSTARLRALGIEVLDANDIESLAVYIDGADEIDHAGCMIKGGGAALTREKIIADLA
                     ARFICIADESKLVKTLGRFPLPVEVIPMAAAQIARQLRKLGGEPVLRAGLVTDNGGHI
                     IDVRGLVINDPDAMEREINQWPGVVSCGIFARNKASACLLGTAQGVKTLTF"
     gene            732..914
                     /locus_tag="VFA35_04230"
     CDS             732..914
                     /locus_tag="VFA35_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55411.1"
                     /translation="MAKPNYSFEKRQRELAKKKKKEEKLKEKAGRKAGEAAPDDTPDD
                     EAATDAAPAEVQPPAP"
     gene            complement(932..2734)
                     /gene="mnmC"
                     /locus_tag="VFA35_04235"
     CDS             complement(932..2734)
                     /gene="mnmC"
                     /locus_tag="VFA35_04235"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HZT55412.1"
                     /translation="MSVLLAGAGLPQRWRGRERFVVLDTEFAAGHRFLAAWQAWRDDP
                     LRCARLVFIALQPHVLGRIEPRAALSDAQPAGLADALLCAWPTQTPDLHRLSFDDGRV
                     VLLLAPGDPARWLRELVAEIDAFCVAGAALANAERVCKAFGRLAAPGATLAAGADDIT
                     TLRAPLTSAGFEFRDGPEGGLMTARFAPAFAPRRVPACARDAVERHALIVGAGIAGCA
                     AAWALAEQGWRSTLLERGPRIAGAASGNPAGLFHGIVNAQDGTHARFNRAAALAAHGA
                     VQAAIERGAAGSARGLLRLEAKLDVAAMRALLQRLALPPEYVCALAAGEASEHAGITL
                     AQPCWYYPGGGWVDPAGLARSFLDRAGAAAQLRTGADAHRLEPGAAGWRVLDARGQLL
                     AEAQVVVLANAGDAPRLLDAPGWPIDSIRGQISIAPASALVSARLPVAGSGYLLPATD
                     GKVMFGATAQRGDPDPAVREADHGLNLAQLERLLGHATGLRPAELEGRTGWRCSAADR
                     LPVIGAVPDPSARGLHLDRPRLVPRQRGLYVFTALGSRGITWSALGAEVLASWVTGAP
                     APIGAGLLDAIDPARFVSREARRFRREPALGAGA"
     gene            complement(2731..3666)
                     /locus_tag="VFA35_04240"
     CDS             complement(2731..3666)
                     /locus_tag="VFA35_04240"
                     /inference="COORDINATES: protein motif:HMM:NF023354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent oxidative N-demethylase subunit
                     alpha family protein"
                     /protein_id="HZT55413.1"
                     /translation="MPFDFATVSAPFRMQPGLRRIAPGARQLTPNALGDRALREKLGV
                     LRQHPAQALLAAPGFDAAPALNALLRQAATEHADAFAWDGALALDATRLRWSVRGSAV
                     HGDGPAEIGACLRALPPEWRLPGLLSLALAEDFAVIDGATARIPWLAVCLPSRWAPEE
                     KIGRHFAEVHAPVADNQVLLAAADHLARLVTGTDRWERFVWTINAEPRLMQHPQHVPP
                     TAWPAALDAEGLAAVASFRTEHQTFIPLADAHQAVFTIGVDSQPLIEAVDSPERARRL
                     HDALASMSDAVLAYRGLTDARDRLLAWLAATATAA"
     gene            complement(3736..>3963)
                     /locus_tag="VFA35_04245"
     CDS             complement(3736..>3963)
                     /locus_tag="VFA35_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZT55414.1"
                     /translation="VEVVFYDGKAISVELPTTLVREIADTEPAVKGDTSGKVLKSARL
                     KTGLEIAVPLFVQTGDKVEIDTRTHEYRKRV"
BASE COUNT          593 a         1435 c         1466 g          469 t
ORIGIN      
        1 cctggtcggg caggccgcca ttgcttatgt cgagccgggc acggtgatcg gcgtgggcac
       61 cggctcgacg gtgaactgct tcatcgatgc gctggcgcag atgccggtgc gcattgcggc
      121 ggcggtgtcg agctcgcagc agagcacggc ccggctgcgc gcgctcggca tcgaggtgct
      181 cgatgcgaac gacatcgaat cgctcgccgt ctacatcgac ggcgccgacg agatcgacca
      241 cgccggctgc atgatcaagg gcggcggcgc ggcgctgacg cgcgagaaga tcatcgccga
      301 cctggcggcg cgcttcatct gcatcgccga cgaatcgaag ctggtcaaga cgctggggcg
      361 gtttccgctg ccggtcgagg tgatcccgat ggcggcggcg cagatcgcgc ggcagctgcg
      421 caagctgggc ggcgagccgg tgctgcgcgc cggcctggtc accgacaacg gcggccacat
      481 catcgacgtg cgcggcctcg tcatcaatga cccggacgcg atggagcgcg aaatcaacca
      541 gtggccgggg gtcgtgagct gcggcatctt cgcgcgcaac aaggcgagcg cctgcctgct
      601 cggcaccgcg caaggcgtca agacgctgac tttctgacgc cgctgaaaat cgcgccgccg
      661 aaaagcgcgt aacctcgggc ctggcgcagc gttggcgccc gactcgacac ccgacacacg
      721 aaggacggcc catggccaaa ccaaattact cattcgaaaa acgccagcgg gaactggcga
      781 agaagaagaa gaaggaagag aagctgaagg agaaggccgg ccgcaaggcc ggcgaggccg
      841 cccccgacga cacccccgac gacgaagccg cgaccgacgc ggcaccggcc gaggtccagc
      901 cgcccgcgcc ctgagaagct ctcgctgacg gctacgcgcc cgccccgagc gccggttctc
      961 gacggaaccg ccgcgcctcg cggctgacga agcgggccgg gtcgatcgca tcgagcaggc
     1021 cggcgccgat cggcgccggc gcgccggtca cccacgacgc aagcacttcg gcaccgagcg
     1081 cactccaggt gatgccgcgc gagccgagcg cggtgaacac gtacaggccg cgctggcgcg
     1141 gcacgaggcg cggccgatcc aggtgcaggc cccgtgcgct ggggtcgggc actgcgccga
     1201 tcaccggcaa gcgatcggcg gcgctgcagc gccagccggt gcggccctcg agctcagccg
     1261 gccgcaagcc ggtcgcatgg ccgagcagac gttccagttg cgcgaggttc agcccatggt
     1321 cggcctcgcg cacggccggg tcggggtcgc cgcgctgcgc ggtcgccccg aacatgacct
     1381 tgccgtcggt ggcgggcaac aggtagccgc tgccggccac cggcagccgc gccgagacca
     1441 gcgccgacgc gggcgcgatg ctgatctggc cgcggatgct gtcgatcggc cagccggggg
     1501 cgtcgagcag gcgcggcgca tcgccggcgt tcgcgagcac gacgacctgc gcctcggcga
     1561 gcaactggcc gcgcgcatcg agcacgcgcc agccggccgc gccgggttcg aggcgatgcg
     1621 catcggcgcc ggtgcgcagt tgcgccgcgg cgcccgcgcg gtcgagaaag ctgcgcgcca
     1681 gaccggccgg atcgacccag ccaccgccgg ggtagtacca gcacggctgc gccagcgtga
     1741 taccggcatg ctcgctggcc tcgccggcgg cgagcgcgca gacgtactcg ggcggcagcg
     1801 cgagccgctg cagcagcgcg cgcattgcgg cgacatcgag cttcgcttcc agccgcagca
     1861 acccgcgcgc gctgccggcg gcaccacgct cgatcgcggc ctgcaccgcg ccgtgcgcgg
     1921 caagcgccgc ggcgcggttg aagcgggcgt gcgtgccgtc ctgcgcattg acgatgccgt
     1981 ggaacaagcc ggccgggttg ccggacgccg cgccggcgat gcgcggcccg cgttcgagca
     2041 aggtgctgcg ccagccctgt tccgccagcg cccacgcggc ggcgcagccg gcgataccgg
     2101 cgccgacgat cagcgcgtgc cgttcgactg catcgcgcgc gcacgccggc acacgccgcg
     2161 gcgcgaacgc cggcgcgaac cgtgcggtca tcaacccgcc ctcgggcccg tcgcggaact
     2221 cgaagcccgc gctggtcagc ggcgcgcgca gcgtggtgat gtcgtcggcg ccggcggcca
     2281 gcgtcgcgcc cggcgcggcg aggcggccga aggccttgca cacgcgctcg gcgttggcca
     2341 gcgcagcgcc ggcaacgcaa aacgcgtcga tctcggcgac cagctcgcgc agccatcgcg
     2401 cggggtcacc gggcgcgagc agcagcacga cccggccgtc gtcgaagctc aggcggtgca
     2461 ggtcgggggt ttgcgtcggc cacgcgcaca acagcgcgtc ggccagcccg gcgggctgtg
     2521 cgtcgctcaa cgccgcgcgc ggttcgatcc gacccagcac gtgcggctgg agcgcgatga
     2581 agacgaggcg tgcgcagcgc agtgggtcgt cgcgccatgc ctgccacgcg gcgagaaagc
     2641 ggtggccggc cgcgaattcg gtgtcgagca cgacgaagcg ctcgcggccc cgccagcgct
     2701 gcggcaagcc ggcgccggcc agcaagacgc tcatgctgcc gtggccgtgg ccgcgagcca
     2761 cgccagcagg cgatcgcgcg cgtcggtcag cccgcggtac gcgagcaccg cgtcgctcat
     2821 gctcgcgagc gcgtcgtgca gccggcgcgc gcgctcggga ctgtcgaccg cttcgatcaa
     2881 cggctggctg tcgaccccga tcgtgaagac cgcctggtgc gcatcggcca gcgggatgaa
     2941 ggtctggtgc tcggtgcgaa agctcgccac ggccgcgagc ccctcggcat cgagcgccgc
     3001 cggccacgcg gtcggcggca cgtgctgcgg atgctgcatc aggcgcggct cggcgttgat
     3061 cgtccagacg aagcgctccc agcgatcggt gccggtgacg agccgcgcga ggtggtcggc
     3121 ggcggcgagc agcacctggt tgtcggcgac cggcgcgtgc acctcggcga agtgccggcc
     3181 gatcttctct tcgggcgccc agcgcgacgg caggcagacc gcgagccacg ggatgcgcgc
     3241 ggtcgcgccg tcgatcaccg cgaagtcctc ggccagcgca aggctcagca ggccgggcag
     3301 gcgccactcg ggcggcagcg cgcgcaggca ggcgccgatc tcggccgggc cgtcgccgtg
     3361 cacggcgctg ccgcgcacgg accagcgcag gcgcgtcgcg tcgagggcga gggcgccgtc
     3421 ccacgcgaac gcgtcggcgt gctcggtcgc ggcctgccgc aacagcgcgt tcagcgccgg
     3481 cgccgcgtcg aaaccggggg cggcgagcag cgcctgcgcc gggtgctgcc gcagcacgcc
     3541 gagcttctcg cgcagcgcgc ggtcgcccag cgcattgggc gtgagctggc gcgcgcccgg
     3601 cgcgatccgc cgcaggcccg gctgcatccg aaacggcgcg ctgacggtgg cgaaatcgaa
     3661 cggcatgcgc gaatcatgag gcaccaaaca gaagggcccg caaagcgggc ccgccgtcgt
     3721 ctcaggggtt tgtactcaaa cccttttccg atactcgtgc gtgcgcgtgt cgatctcgac
     3781 cttgtcgccg gtctggacga agagcggcac cgcgatctcg agcccggtct tcagccgcgc
     3841 gctcttcagc accttgcccg acgtgtcgcc cttgacggcc ggctcggtgt cggcgatctc
     3901 gcgaacgagc gtcgtcggca gctcgaccga gatcgccttg ccgtcgtaga agacgacctc
     3961 gac
//
LOCUS       DASRDG010000256         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_151942, whole genome shotgun sequence.
ACCESSION   DASRDG010000256 DASRDG010000000
VERSION     DASRDG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151942"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1599)
                     /locus_tag="VFA35_04250"
     CDS             complement(223..1599)
                     /locus_tag="VFA35_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55415.1"
                     /translation="MTAAGVELARDPVAWLQAHGGWLFERELGACIVGSHALAIACRR
                     AGIAAPTPRDLDLAWSLDVDEGRALLERRGVFVPTTDGSVQRGTLALKIDHQRLEVTA
                     FRDARPRNPLAQRIADDAAARDMTVGALAVELATGVVHDPTGGLGHWQQRRIVPVGDP
                     GERVREHPVRWLRYYRKAHEWSFELDRSVRRLRLPPHVLASVPREALGAELRAALLYC
                     ASPGRFFVDLHEIGVLAWLAPELDLQFDGRPAGPQRWHPEIAQSLHLVLALEWAAANS
                     QHLDERDRLAVMVAVLCHDLGKGFTDAAELPGHPGHERLGVPHVHAFLRRFPGLLDRR
                     GAALAQDVCALHVEIRGMHELRPGTLARLYDQHFRARDYPIEPFALAVAADSGGRLGL
                     ADAGRRVHEQVASDLRWLRRCCEAIDATALRTRCADLAAFRAALHEARARALGGGPPR
                     AQKPDAKA"
     gene            complement(1596..>2015)
                     /locus_tag="VFA35_04255"
     CDS             complement(1596..>2015)
                     /locus_tag="VFA35_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55416.1"
                     /translation="PEIVRLLKSNGTQGQLAVQAALVMALAQCGYQTADWAAVGRLFG
                     PDYPAERVPLQEAILGLAAQHKEKQSIELLLDNIDEPVPQNVDAANNPPASYWEARWK
                     AWKAWREKVKDALFALTGQRFGSAAEARAWLAKNPLK"
BASE COUNT          251 a          741 c          764 g          259 t
ORIGIN      
        1 tcggcggcaa cttcacgctg aacgggctgt cgccgggccg ctacaaggtg cgcgcgcagc
       61 agctcggccc cggcgagtcg gcgcccggcc ccgaggtcga agtcgaggtc gccgcgggca
      121 aggtcgcggg cggcgtcgaa ctgcgcgtgg cagcaccccg ctgaccgtcg cgctgaccgt
      181 cgcgctgcca tcgtgtttgc cgtcgcgctg gcttgggccg cgtcacgcct tcgcgtcagg
      241 cttctgagcg cgcggcgggc cgccgccgag cgcgcgcgcg cgcgcctcgt gcagcgccgc
      301 ccggaaggcg gcgaggtccg cgcagcgggt gcgcagcgcc gtggcgtcga tcgcctcgca
      361 gcagcggcgc agccagcgca gatcgctggc cacctgttcg tgcacgcgcc gccccgcgtc
      421 ggcgaggccg aggcggccgc cagaatcggc ggcgaccgcg agcgcgaacg gttcgatcgg
      481 gtagtcgcgc gcgcggaagt gctggtcgta gaggcgggcg agcgtgccgg ggcgcagttc
      541 gtgcatgccg cggatctcga cgtgcagcgc gcagacgtcc tgcgccagcg ccgcgccgcg
      601 ccggtcgagg aggccgggga agcggcgcag gaaggcatgg acgtgcggca cgccgagccg
      661 ttcgtggccg gggtggcccg gcagctcggc ggcgtcggtg aagcccttgc cgaggtcgtg
      721 gcacagcacc gccaccatca cggcgagccg gtcgcgttcg tcgaggtgct gcgagttggc
      781 cgcggcccac tcgagcgcga gcacgaggtg cagcgactgc gcgatctccg ggtgccagcg
      841 ctgcgggccc gccggccggc cgtcgaactg gaggtcgagc tccggcgcga gccacgcgag
      901 cacgccgatc tcgtgcaggt cgacgaagaa gcgccccggc gaggcgcagt agagcagcgc
      961 cgcgcgcagc tcggcgccca gcgcctcgcg cggcacggac gcgagcacgt gcggcggcag
     1021 gcgcaggcgg cgcacgctgc ggtcgagctc gaacgaccat tcgtgtgcct tgcggtagta
     1081 gcgcagccag cgcaccggat gctcgcgcac gcgctcgcca gggtcgccga cgggcacgat
     1141 ccgccgttgc tgccagtgcc cgaggccgcc cgtcgggtcg tgcacgacgc cggtcgcgag
     1201 ctcgaccgca agcgcgccga ccgtcatgtc gcgcgccgcc gcgtcgtcgg cgatgcgctg
     1261 cgccagcggg ttccgcggcc gcgcatcgcg gaacgcggtg acctcgagcc gctggtggtc
     1321 gatcttgagc gccagcgtgc cgcgctgcac gctgccgtcg gtggtgggca cgaacacgcc
     1381 gcggcgctcg agcagcgcgc ggccctcgtc gacgtcgagc gaccacgcga ggtcgaggtc
     1441 gcgcggcgtc ggcgccgcga tccctgcgcg ccggcaggcg atcgcgagcg cgtgactgcc
     1501 gacgatgcat gccccgagct cgcgctcgaa cagccagccc ccgtgcgcct gcagccaggc
     1561 gaccggatcg cgcgcgagtt cgacgccggc ggcggtcact tcagcgggtt cttggccagc
     1621 caggcccgcg cctcggcggc ggaaccgaag cgctggcccg tgagcgcgaa cagcgcgtcc
     1681 ttgaccttct cgcgccacgc cttccatgcc ttccagcgcg cctcccagta gctcgccggc
     1741 gggttgttgg ctgcatcgac gttctgcggc accggctcgt cgatgttgtc gagcagcagc
     1801 tcgatggact gcttctcctt gtgctgcgcc gcgaggccga ggatcgcctc ctggagcggc
     1861 acgcgttcgg ccgggtagtc cgggccgaac agccggccga ccgcagccca gtccgccgtc
     1921 tggtagccgc actgcgcgag tgccatgacc aacgcggcct gcaccgcgag ctggccctgc
     1981 gtgccattgc tcttcagcaa ccgcacgatc tccgg
//
LOCUS       DASRDG010000257         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1190777, whole genome shotgun sequence.
ACCESSION   DASRDG010000257 DASRDG010000000
VERSION     DASRDG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190777"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFA35_04260"
     CDS             <1..704
                     /locus_tag="VFA35_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZT55417.1"
                     /translation="MIAAGFLAAGARRVYISSRKAADCAATAQELSAQGECIALPADV
                     SSMEGIEALAAAYTAREATLDILVNNAGAAWGEAFDTFPEKGWDKVVDLNLKTPFFLT
                     QALIGPLRAAAKTRPAKVINIASIDGISINPQETYSYAASKAGLIQLTRRMALRLAED
                     NIVVSSIAPGAFASEMNRVARDHEEETAKRIPAKRIGRDEDMAGGAIYLASRAGDYVM
                     GSTLVIDGGVTHARG"
     gene            782..1195
                     /locus_tag="VFA35_04265"
     CDS             782..1195
                     /locus_tag="VFA35_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HZT55418.1"
                     /translation="MNAILAPSAPPVELDSAAAAQAALRTFWRIGEAWQLDATEQTTL
                     LGVGRTTLYQWKQGKVGAGLDRHVLERLSYLFGIYAALQILLPDGSQADAWVRKPNAA
                     PLFGGKSALARMLGGQVADLYVVRHYLDAQRGGKA"
     gene            1195..1899
                     /locus_tag="VFA35_04270"
     CDS             1195..1899
                     /locus_tag="VFA35_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HZT55419.1"
                     /translation="MSVAPKSIPLSVTEWRPSYRLIPSRYPTVGLYDRVADPADLDVV
                     FAIEALTNPRIRDEIGQLQLVPPQERVSGAGSTMVMAAFTHLNPEGSRFSDGSYGVYY
                     AAHALDTAVAEVSHHRALFLSRTNEPALDIDLRVIAATVNAELHDLRGLGKRGVALLD
                     PNRYAAPQTLGRQLRAAGSWGVVFPSVRHAGGVCVGVFRPKALRQARSGAHIALHWDG
                     ARITHWYEKRGPHALP"
     gene            1964..>2301
                     /locus_tag="VFA35_04275"
     CDS             1964..>2301
                     /locus_tag="VFA35_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HZT55420.1"
                     /translation="MSDRLKVLPQYLLPKQALTRYAGWRASREWGARTTRLIGGFVAK
                     YGVDMSEAADPDIASYKSFNAFFTRALKPGARPLAAADLICPVDGAVSQFGAIKNDQI
                     FQAKGHAYSTT"
BASE COUNT          368 a          823 c          775 g          335 t
ORIGIN      
        1 gcatgatcgc ggcgggcttt ctcgcggcgg gcgcccggcg tgtctacatc tcgtcgcgca
       61 aggcggccga ctgcgccgcg accgcgcagg agctgtcggc acaaggcgag tgcatcgcgc
      121 tgccggccga cgtgtcgtcg atggaaggca tcgaggcgct ggcggcggcc tacacggcac
      181 gcgaggcgac gctcgacatc ctcgtcaaca acgccggtgc ggcctggggc gaggccttcg
      241 acaccttccc cgagaaaggc tgggacaagg tcgtcgacct gaacctgaag acgccgttct
      301 tcctgacgca ggcgctgatc gggccgctgc gcgccgccgc gaagacgcga ccggccaagg
      361 tcatcaacat cgcctcgatc gatggcatct cgatcaaccc gcaggagacc tactcctacg
      421 ccgcgagcaa ggccggcctg atccagctga cgcgacgcat ggcactgcgc ttggccgaag
      481 acaacatcgt cgtcagcagc atcgcgccgg gcgcgttcgc ctccgagatg aaccgcgtcg
      541 cgcgcgacca tgaagaagag accgccaaac gcatccccgc caaacgcatc ggccgcgacg
      601 aagacatggc gggcggcgcg atctacctgg cctcgcgcgc cggcgactac gtgatgggct
      661 cgacgctggt gatcgacggc ggcgtgacgc acgcgcgggg gtaagccagt gctgaaccat
      721 ttgcccgttc gttttcggac aaattattga tattcgtccg taggtggact acccttcgtc
      781 catgaacgcg atcctcgcgc ccagcgcacc gcccgtcgag ctcgattccg ccgccgctgc
      841 gcaggccgca ttgcgcacct tttggcgcat cggcgaggcc tggcagctcg acgccaccga
      901 gcagaccacg ctgctcggcg tcggccgcac cacgttgtac cagtggaagc agggcaaggt
      961 cggcgccggg ctcgatcgcc acgtgctgga gcgcctgtcc tacttgttcg gcatctacgc
     1021 cgcgctgcag atcctgctac cggacggcag ccaagccgac gcgtgggtgc gcaagccgaa
     1081 cgccgcgccg ctgttcggcg gcaagtcggc gctggcgcgc atgctgggcg ggcaggtcgc
     1141 tgacctgtac gtggtgcgcc actacctcga tgcgcagcgg ggcggcaagg cctgatgtcg
     1201 gttgcgccga agagtatccc gctgagcgtc acggagtggc gccccagtta ccgcctgatt
     1261 ccgtcccgct acccgaccgt cggtctctac gaccgcgtcg ccgatccggc cgacctcgac
     1321 gtcgtgttcg cgatcgaggc gctgacgaat ccgcgcatcc gcgacgagat cggccagttg
     1381 caactcgtgc cgccgcagga gcgcgtcagc ggcgccggca gcacgatggt gatggcggcg
     1441 ttcacgcacc tgaatccgga aggctcgcgc ttctcggacg gcagctacgg cgtctactac
     1501 gccgcgcacg cgctggacac ggcggtggcc gaagtcagcc accaccgcgc gctgttcctg
     1561 tcgcgcacga acgagccggc gctcgacatc gacctgcgcg tgatcgccgc gaccgtcaac
     1621 gccgagctgc acgacctgcg cggcctcggc aagcgcggcg tcgcgctgct cgacccgaac
     1681 cggtacgcgg ccccgcagac gctcggccgc cagctgcgcg cggccggcag ttggggcgtg
     1741 gtgttcccga gcgtgcgcca tgcgggcggc gtgtgcgtcg gcgtgttccg gccgaaagcg
     1801 ctgcgccagg cccgctcggg cgcgcacatc gcgctgcact gggacggcgc gcgcatcacc
     1861 cactggtacg agaagcgcgg gccgcacgcg ctgccgtgat gcgacccgat gaagacacgc
     1921 gcgtcgatac aatcgcgccc tcctgtcacc cgccgcgccg cccgtgtccg accgcctgaa
     1981 agtgctcccg caatacctgc tgccgaagca ggcgctgacg cgctatgccg gctggcgcgc
     2041 gtcgcgcgaa tggggcgcgc gcacgacccg gctgatcggc ggctttgtcg cgaaatacgg
     2101 cgtcgacatg agcgaggccg ccgaccccga catcgcgagc tacaagagct tcaacgcgtt
     2161 cttcacgcgc gcgctcaagc ccggtgcgcg gccgctcgcg gcggccgacc tgatctgccc
     2221 ggtcgacggt gcggtgagcc agttcggcgc gatcaagaac gaccagatct tccaggccaa
     2281 gggccatgcg tactcgacca c
//
LOCUS       DASRDG010000258         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_269715, whole genome shotgun sequence.
ACCESSION   DASRDG010000258 DASRDG010000000
VERSION     DASRDG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269715"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1457
                     /locus_tag="VFA35_04280"
     CDS             99..1457
                     /locus_tag="VFA35_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZT55421.1"
                     /translation="MTSSTVTADDRLLRAPVGAAVLRLGLPLAFGMASHALVNLVDLA
                     LVGQLGPDAVAAAHVASTINFLPMILGNGISVATLGALSRLLGNGDRAAAAGFARRAL
                     WFALWCGALVGVATALPAAPCVDSVGVTGAVRSGAVHYLVVSNLGCLSMFALMQATAL
                     MRAAGETLMPIVLLIGANLLNLAFSVVLLFGWPALGIPSVGVAGAAYAAVAARTAAAV
                     VGVLWLLRARHPLRLRGGRGGPPVAGVLLAGAWPQCLQIGLRAALVWVLTVFVQQKSG
                     DAGVAAIGVTTRLDTMVLFAGVGFASAATTLAGRAVSAGMTDRARAAGLWAGAQALVF
                     GSLLVSLFRWFAVPILHRFLPDAGAPIVDAGVHYLTIAAVAQPLAACALGAMGAVHGA
                     GRMIPPLLVDLVGFCGLGLAMALAARLELDFVYAAMVGGTALVAALHLVLVRAGRWAV
                     PD"
     gene            1636..>2036
                     /locus_tag="VFA35_04285"
     CDS             1636..>2036
                     /locus_tag="VFA35_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015551592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZT55422.1"
                     /translation="MSKIVGIDLGTTNSVVSVMEGGEPKVIANMEGGRTTPSVVAFTP
                     SGERLVGAPAKRQAITNPANTIYSIKRFMGRRHREVGDEEKMVPYKVVGGAEELVKVE
                     VNGQQYTPPQISADILRSLKDAAEAYLGEKV"
BASE COUNT          225 a          734 c          758 g          319 t
ORIGIN      
        1 cgcgacctgc tgggcccgac cgtcgtccac ttctgctggc gcacggacgg cggcaccgag
       61 ccgccgcgcc gctcggcgct cgactggttc gtgctgcgat gacttcgagc accgtcactg
      121 ccgacgaccg cctgctgcgc gcgcccgtgg gggccgcggt gctgcgcctc ggtctgccgc
      181 tcgcgttcgg catggcgtcg cacgcgctcg tcaacctggt cgacctcgcg ctggtgggcc
      241 agctcggccc cgacgcggtc gccgccgcgc acgtcgcctc gacgatcaac ttcctgccga
      301 tgatcctcgg caacgggatc tcggtcgcga ccctgggcgc gctgtcgcgg ctgctcggca
      361 acggcgatcg cgcggcggcc gccggcttcg cgcgccgcgc gctctggttc gcactctggt
      421 gcggcgccct ggtcggcgtc gccacggcgc tgccggcggc gccgtgcgtc gacagcgtcg
      481 gcgtcaccgg cgcggtgcgc agcggcgccg tgcactacct cgtggtgagc aacctcggct
      541 gcctgtcgat gttcgccctc atgcaggcga ctgcgctcat gcgtgcggcg ggggagacgc
      601 tgatgccgat cgtgctgctg atcggggcca acctgctgaa cctggcgttc agcgtcgtgc
      661 tgctgttcgg gtggccggcg ctcggcatcc cgtccgtcgg cgtcgccggc gcggcgtacg
      721 cggcggtcgc cgcgcgcacc gcggccgccg tcgtgggcgt gctgtggctg ctgcgcgcgc
      781 gccatccgct gcggttgcgc ggcgggcgcg gcgggccgcc ggtcgccggc gtgctgctcg
      841 cgggcgcgtg gccgcagtgc ctgcagatcg gcctgcgcgc ggcgctcgtc tgggtgctca
      901 cggtcttcgt gcagcagaag agcggcgacg ccggcgtcgc ggcgatcggc gtcacgacga
      961 ggctcgacac gatggtgttg ttcgcgggcg tcggcttcgc gagcgcggcg acgacgctgg
     1021 ccggccgcgc ggtgagcgcc ggcatgacgg accgcgcgcg cgccgcgggg ctgtgggccg
     1081 gtgcgcaggc gctggtgttc ggcagcctgc tcgtgtcgct gttccgctgg ttcgccgtgc
     1141 cgatcctgca ccggttcctg cccgacgccg gcgcgccgat cgtcgacgcc ggcgtgcact
     1201 acctcacgat cgcggcggtc gcgcagccgc tggccgcgtg cgcgctcggc gcgatgggcg
     1261 ccgtgcacgg cgccggccgc atgatcccgc cgctgctcgt cgacctggtc ggcttctgcg
     1321 ggctcggcct cgcgatggcg ctcgccgcgc ggctcgagct cgacttcgtc tacgcggcga
     1381 tggtcggcgg caccgcgctg gtcgccgcgc tgcatctggt gttggtgcgc gccggtcgct
     1441 gggcagtgcc agactgacag ccgtgcgtcg ccgcttcgct gccagattgt cggcgagcac
     1501 cctcggctcc gaaccggaac ctgcgtgcat ccaagtgctt gcgtgcttcg ggcgcgaggt
     1561 acgccgtttg actagccgcg cgattcccaa cgcggccagg gtcgccagca cggtcctggc
     1621 caggagacca atcgcatgtc gaagatcgtc ggtatcgacc tcggaaccac caactcggtc
     1681 gtgtcggtga tggagggcgg cgagcccaag gtcatcgcca acatggaggg cggtcgcacc
     1741 acgccgtcgg tcgtcgcctt cacgccgagc ggcgaacggc tggtcggcgc gccggccaag
     1801 cgtcaggcga tcaccaaccc cgcgaacacg atctactcga tcaagcggtt catgggccgc
     1861 cgccaccgcg aggtcggcga cgaggagaag atggtcccgt acaaggtcgt cggcggcgcc
     1921 gaagagctgg tcaaggtcga ggtcaacggc cagcagtaca cgccgccgca gatcagcgcc
     1981 gacatcctgc gctcgctgaa ggacgcggcc gaggcctacc tcggcgagaa ggtcga
//
LOCUS       DASRDG010000259         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1550189, whole genome shotgun sequence.
ACCESSION   DASRDG010000259 DASRDG010000000
VERSION     DASRDG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550189"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFA35_04290"
     CDS             complement(<1..623)
                     /locus_tag="VFA35_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HZT55423.1"
                     /translation="MQIGPIRLPNHLFAAPMAGVTDRPFRQLCKRLGAGYAVSEMVTS
                     RKDLWTSLKTSRRANHDGEAAPIAVQIAGTDAQMMGEAAAYNIDRGAQIIDINMGCPA
                     KKVCNKWAGSALMQDEPLALQIIEAVVAACAPRNVPVTLKMRSGWCEAERNALRIARA
                     AEGAGVAMVTVHGRTRAQGYKGSAEYDTVGAVKAAVRIPVVANGDIDS"
     gene            740..1225
                     /locus_tag="VFA35_04295"
     CDS             740..1225
                     /locus_tag="VFA35_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HZT55424.1"
                     /translation="MEAWVESMLALLALPKFGLSTVFVVSFISATLLPLGSEPAVFGL
                     VKLNPEMFWPAVLVATVGNTLGGAVTYWMGYGAERAYESMSHHKPPNARALRWLGRWG
                     TRGCLLSWLPIVGDPLCAVAGWLNLPFWPCVFYMAIGKFARYVTMTSAMLWFWPGQFT
                     H"
     gene            1314..>2563
                     /locus_tag="VFA35_04300"
     CDS             1314..>2563
                     /locus_tag="VFA35_04300"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HZT55425.1"
                     /translation="MNAAYDELTGIWNRLHHFRHLQAIAGWDQAAMMPPKGNDARAGA
                     MAEMEGLLHGMRTDPKLAGLLRRAAAEGLDDFARANLGEMRREWRAANALPQPLVEAL
                     SLAGARCEHAWRSQRPANDWLGFLENFRPVLALGREVAQRLADATGLAPYVALLDQFE
                     PGTTRADVDRVFGDLQQWLPRLVQQVREKQAGEAVVEPVGPFPKATQRALNHDVMALL
                     GFDFDGGRLDESAHPFSGGVPEDTRLTTRYREDDLMQALMGTIHETGHARYEQNLPRA
                     WLGQPVAHARSMGIHESQSLSFEMQLGRSPAFAGLLAPLLRARFGDQAAFEPAKLHRL
                     MTRVKPAFIRVDADELTYPAHVILRYGIERELIAGSLEADDIPALWDTKMAELLKRDT
                     RGNFKDGCLQDVHWSEGLLGYFPC"
BASE COUNT          357 a          908 c          899 g          399 t
ORIGIN      
        1 gaatcgatgt cgccgttcgc gaccaccggg atgcgcaccg cggccttgac agcaccgacc
       61 gtgtcgtact cggccgaacc cttgtagccc tgcgcacggg tgcggccgtg caccgtgacc
      121 atcgcgacgc cggcgccctc ggccgcgcgc gcgatgcgca gcgcgttgcg ttcggcctcg
      181 caccagccgc tgcgcatctt cagcgtcacc ggcacgttgc gcggcgcgca ggccgcgacc
      241 acggcttcga tgatctgcag cgcgagcggt tcgtcctgca tcagcgccga gccggcccac
      301 ttgttgcaga ccttcttcgc cgggcaaccc atgttgatgt cgatgatctg cgcgccgcgg
      361 tcgatgttgt aggccgcggc ctcgcccatc atctgcgcat cggtgccggc gatctgcacc
      421 gcgatcggcg cggcctctcc gtcgtggttc gcgcggcggc tggtcttcag gctcgtccac
      481 aggtccttgc gcgaggtcac catctcgctg accgcgtagc ccgcgccgag ccgcttgcac
      541 agctggcgga acggccggtc ggtcacgccc gccatcggcg cggcgaacag gtggttcggc
      601 aatcggatgg ggccgatctg catggggggt ggcgcgcgcg ggggtgctga aaaaggaggc
      661 agaagtatag cggcgggttg cccccgcgcg tcctgcgtcg gggcgatgcg cgcgcggttc
      721 atgttcataa tcccgcccga tggaagcatg ggtcgaatcg atgttggcgt tgctggccct
      781 gccgaagttc ggcctgagca cggtgttcgt cgtctcgttc atctcggcca ccttgctgcc
      841 gctgggctcg gagccggcgg tgttcggcct cgtcaagctc aaccccgaga tgttctggcc
      901 cgcggtgctg gtcgcgaccg tcggcaacac gctgggcggc gcggtgacgt actggatggg
      961 ctacggcgcc gagcgcgcgt acgagagcat gagccaccac aagccgccca atgcgcgcgc
     1021 gctgcgctgg ctcgggcgct ggggcacgag gggctgcctg ctgtcgtggc tgccgatcgt
     1081 tggcgatccc ttgtgcgcgg tcgccggctg gctcaacctg ccgttctggc cctgtgtgtt
     1141 ctacatggcg atcggcaagt tcgcgcgcta tgtcacgatg acgagcgcga tgctctggtt
     1201 ctggcccggg cagttcacac actgaaacat gacgaccccc acgtcgcttc gctcctgccc
     1261 cccgaggggg cgctcatccg ctttggggcg gcccggcggc gcctgagact gcaatgaacg
     1321 ccgcctacga cgaactcacc ggcatctgga accggctgca ccacttccgg cacctgcagg
     1381 cgatcgccgg ctgggaccag gccgcgatga tgccgcccaa gggcaacgac gcgcgcgccg
     1441 gggcgatggc cgagatggag gggctgctgc acggcatgcg caccgacccg aagctggccg
     1501 ggttgctgcg gcgcgccgcc gccgagggcc tcgacgactt cgcgcgcgcc aacctcggcg
     1561 agatgcgccg cgagtggcgc gccgcgaatg cgttgccgca gccgctggtc gaggcgctct
     1621 cgctcgccgg cgcgcggtgc gagcacgcct ggcgcagcca gcggccggcg aacgactggc
     1681 tcggctttct cgagaacttc cgcccggtgc tcgcgctcgg ccgcgaggtc gcgcagcgcc
     1741 tggccgacgc gaccggcctt gcgccctacg tcgcgctgct cgaccagttc gagcccggca
     1801 ccacgcgcgc cgatgtcgac cgtgtgttcg gcgatctgca gcagtggctg ccgcgcctcg
     1861 tccagcaggt gcgcgagaag caggccggcg aggcggtggt cgagccggtc ggcccgttcc
     1921 cgaaagccac gcagcgcgcg ctcaaccacg acgtgatggc gctgctcggt ttcgacttcg
     1981 acggcggccg gctcgacgag agcgcgcacc cgttcagcgg cggcgtgccc gaagacacgc
     2041 gcctgaccac gcgctaccgc gaagacgacc tgatgcaggc gctgatgggc acgatccacg
     2101 agaccggcca cgcgcgctac gagcagaacc tgccgcgcgc gtggctcggg cagccggtcg
     2161 cgcacgcgcg ctcgatgggc atccacgaga gccagagcct gagcttcgag atgcagctcg
     2221 gccgcagccc ggcgttcgcc ggcctgctgg cgccgctgct gcgcgcgcgc ttcggcgatc
     2281 aggccgcgtt cgagcccgcc aaactgcacc ggctgatgac gcgcgtcaaa cccgctttca
     2341 tccgcgtcga tgccgacgag ctgacctacc cggcgcacgt gatcctgcgc tacggcatcg
     2401 aacgcgagct gatcgccggc agcctcgagg ccgacgacat cccggcgctg tgggacacga
     2461 agatggccga gctgctgaag cgcgacacgc gcggcaactt caaggacggc tgcctgcagg
     2521 acgtgcactg gagcgaaggg ctgctcggct acttcccgtg cta
//
LOCUS       DASRDG010000260         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1421989, whole genome shotgun sequence.
ACCESSION   DASRDG010000260 DASRDG010000000
VERSION     DASRDG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421989"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2067
                     /gene="infB"
                     /locus_tag="VFA35_04305"
     CDS             <1..2067
                     /gene="infB"
                     /locus_tag="VFA35_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZT55426.1"
                     /translation="LVAKKPEEPKPAATEIKGTIHKKAAVPGAATTTAAAKPGDKKAV
                     KSEKLSSSWADDAKKRSALKPGQRAPAGGNRPGWRAPRVGRRGERSDGSPSTFVAPQE
                     AQVQEVHVPETISVADLAHKMSVKASEVIKQLMKLGQMVTINQQLDQETAMIVVEEMG
                     HTAFAAKLDDPDAFLEEEAADTAHELLPRAPVVTVMGHVDHGKTSLLDYIRTSRVAAG
                     EAGGITQHIGAYHVDTPGGMITFLDTPGHAAFTAMRARGAKATDIVILVVAADDGVMP
                     QTKEAIHHAKAAGVPIVVAMNKIDKPEANVERLKSELVAEEVVPEDFGGDSPFVAVSA
                     RTGQGIDTLLEQVLLQAEVLELKAATDSMAKGLVIEAQLDKGRGPVATVLVQSGTLKR
                     GDVVLAGSSFGRVRAMLDENGKPISEAGPSIPVEIQGLTEVPQAGDEFMVLGDERRAR
                     EIATFRQGKYRDVKLAKRQAANLENLMDQMGQGATQTLPLIIKADVQGSQEALAQSLL
                     KLSTAEVKVQIVHAAVGGISESDVNLAIASKAVIIGFNTRADAGSRKLAENNGVDIRY
                     YNIIYDAVDEVKAAMTGMLAPEQREEVIGTAEIRQVFVASKIGTVAGSMVTAGVVRRG
                     ARFRLLREHVVVYTGEIESVRRLKDDVREVAAGFECGIKLKNYNDIKEGDQLEFFEVK
                     EVARTL"
     gene            2138..2503
                     /gene="rbfA"
                     /locus_tag="VFA35_04310"
     CDS             2138..2503
                     /gene="rbfA"
                     /locus_tag="VFA35_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZT55427.1"
                     /translation="MRHKRSIPNRGFRVADQIQRDVAELIRDLKDPRIGMVTINAVEV
                     TPDYAHATVLFSLLIGDPAECEIALNEAAGFIRNGLFKRLQIHTVPTLHFKFDRTTER
                     ALELSELIAKANATRAKED"
     gene            2506..3459
                     /gene="truB"
                     /locus_tag="VFA35_04315"
     CDS             2506..3459
                     /gene="truB"
                     /locus_tag="VFA35_04315"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZT55428.1"
                     /translation="MQPQRVRTPKRAVHGVLLLDKPLGLSSNDALQKAKRLYRAEKAG
                     HTGTLDPLATGLLPLCFGAATKFSQVSLDADKRYLTTLKLGVTTTTADAEGEVLETRD
                     VNVTRAQLQAACARFTGPIQQVPPMHSALKRDGKALYEYARAGIEVEREARAVTIHAI
                     DIVGGQDDTWNLDVRCSKGTYIRTLAEDIGAALGCGAHLAALRRTGSGALSLDGALTL
                     EQLGDMSEAERDAALRDADALLADWPVVRLGTEDAGRFLTGLRRRTDLPDAANVRVYG
                     PEPRAFLGGGHIAAGELISTRLLSPVEVQGLLNPTQAVTTP"
     gene            3456..>4102
                     /locus_tag="VFA35_04320"
     CDS             3456..>4102
                     /locus_tag="VFA35_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZT55429.1"
                     /translation="MTRQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRENEKVAERVMD
                     NNDLEKERGITILSKNCAVEWKGTHVNIVDTPGHADFGGEVERVLSMVDSVLLLVDAV
                     EGPMPQTRFVTKKALALGLNPIVVVNKVDRPGARPDYVINATFDLFDKLGANEQQLDF
                     PVIFASGLNGWATLTQGEVGTDLAPLFDTILSHVPAHAGEPDAPLQLQICSLDYST"
BASE COUNT          762 a         1412 c         1375 g          553 t
ORIGIN      
        1 ctcgtcgcca agaagcccga ggagcccaag ccggcagcga ccgagatcaa gggcacgatc
       61 cacaagaagg ccgccgtgcc gggcgccgcg accacgaccg ccgccgcgaa gccgggcgac
      121 aagaaggccg tcaagtccga gaagctctcg tcgagctggg ccgatgatgc caagaagcgc
      181 agcgcgctga agccgggcca gcgcgcaccg gcgggcggca accgcccggg ctggcgcgcg
      241 ccgcgtgtcg gccgccgcgg cgagcgcagc gacggttcgc cgtcgacctt cgttgcgccg
      301 caggaagcgc aggtgcagga agtgcacgtg cccgagacca tcagcgtggc cgatctggcg
      361 cacaagatgt cggtgaaggc ctccgaggtg atcaagcagc tgatgaagct gggccagatg
      421 gtcacgatca accagcagct cgaccaggag accgcgatga tcgtggtcga ggaaatgggc
      481 cacaccgcgt tcgccgccaa gctcgacgac cccgatgcct tcctcgagga agaagcggcc
      541 gacaccgccc acgaactgct gccgcgcgcg ccggtcgtga ccgtgatggg ccacgtcgac
      601 cacggcaaga cttcgctgct cgactatatc cgcacctcgc gggtggccgc gggcgaagcc
      661 ggcggcatca cccagcacat cggcgcgtac cacgtcgaca caccgggcgg catgatcacc
      721 ttcctcgaca cgccgggcca cgccgcgttc accgcgatgc gggcgcgcgg cgcgaaggcg
      781 accgacatcg tgatcctcgt ggtcgcggcc gatgacggcg tgatgccgca gaccaaggaa
      841 gcgatccacc acgcaaaggc ggccggcgtg ccgatcgtcg tcgcgatgaa caagatcgac
      901 aagccggagg cgaacgtcga gcgcctgaag tccgagctcg tggccgagga ggtcgtgccc
      961 gaggacttcg gcggcgattc gccgttcgtc gccgtgtcgg cacgcaccgg ccagggcatc
     1021 gacacgctgc tggagcaggt gctgctgcag gccgaagtgc tggaactgaa ggccgcgacg
     1081 gactcgatgg ccaagggcct cgtgatcgaa gcgcaactcg acaagggccg cgggccggtc
     1141 gcgaccgtgc tggtgcaaag cggcacgctc aagcgcggcg acgtggtgct ggccggttcg
     1201 agcttcggcc gcgtccgcgc gatgctcgac gagaacggca aaccgatcag cgaagccggc
     1261 ccgtcgatcc cggtcgagat ccagggcctg accgaggtgc cgcaagccgg cgacgagttc
     1321 atggtgctgg gcgacgagcg ccgcgcgcgc gagatcgcga ccttccgcca gggcaagtac
     1381 cgcgacgtga agctggccaa gcgccaggcc gcgaacctcg agaacctgat ggaccagatg
     1441 ggccagggcg cgacccagac cttgccgctg atcatcaagg ccgacgtgca gggctcgcag
     1501 gaggcgttgg cgcaatcgct gctcaagctc tcgaccgccg aggtcaaggt gcagatcgtg
     1561 cacgccgcgg tcggcggcat cagcgagtcc gacgtcaacc tggcgatcgc ctcgaaggcc
     1621 gtcatcatcg gcttcaacac gcgcgccgat gccggctcgc gcaagctcgc cgagaacaac
     1681 ggcgtcgaca tccgctacta caacatcatc tacgacgcgg tcgacgaggt gaaggcggcg
     1741 atgaccggca tgctcgcccc cgagcagagg gaggaggtca tcggcaccgc cgagatccgc
     1801 caggtgttcg tcgcctcgaa gatcggcacg gtcgccggct cgatggtcac cgccggcgtg
     1861 gtgcgccgcg gcgcgcgttt ccgcctgctg cgcgagcacg tggtcgtcta caccggcgag
     1921 atcgaatcgg tgcgccgcct gaaggacgac gtgcgcgagg tcgcggccgg cttcgaatgc
     1981 ggtatcaagc tgaagaacta caacgacatc aaggaaggcg accagctcga attcttcgaa
     2041 gtcaaggaag tggcccgaac gctataaggc ggttttggtc tcgtccgaac cccgccctcc
     2101 aaaagacggc ggggtttgtt tttgaaggca cccatccatg cgacacaaac gctccattcc
     2161 caaccgcggc ttccgcgttg ccgaccagat ccaacgcgat gtggccgagc tgatccgcga
     2221 cctgaaggac ccgcgcatcg gcatggtcac gatcaacgcg gtcgaggtca cgcccgacta
     2281 cgcgcacgcg accgtcctgt tctcgctgct gatcggggac ccggccgagt gcgagatcgc
     2341 gctcaacgaa gccgcgggct tcatccgcaa cggcttgttc aagcggctgc agatccacac
     2401 cgtgccgacg ctgcatttca agttcgaccg cacgaccgag cgcgcgctgg aactcagcga
     2461 actgatcgcg aaggccaacg cgacgcgggc caaggaagat tgaaggtgca gccgcaacgc
     2521 gtccgcacgc cgaagcgcgc cgtccatggc gtgctcttgc tcgacaagcc gctcggcctg
     2581 tcgagcaacg atgcgttgca aaaggccaaa cgcctgtacc gcgccgagaa ggccggccac
     2641 accggcacgc tcgacccgct cgcgaccggc ttgctgccgc tgtgcttcgg cgcggcgacc
     2701 aagttctcgc aggtcagcct cgacgccgac aagcgctacc tcacgacgct gaagctcggc
     2761 gtcacgacga cgaccgccga cgccgagggt gaggtgctcg agacgcgcga cgtgaacgtc
     2821 acccgcgccc agctgcaagc cgcgtgcgcg cgcttcaccg ggccgatcca gcaggtgccg
     2881 ccgatgcact cggcgctgaa gcgcgacggc aaggcgctgt acgaatacgc ccgcgccggc
     2941 atcgaggtcg agcgcgaggc gcgcgcggtg acgattcacg cgatcgacat tgtcggcggg
     3001 caggatgaca cttggaacct cgacgtgcgt tgctccaagg gcacctacat ccgcaccctc
     3061 gccgaggaca tcggcgccgc gctcggttgc ggcgcgcacc tggccgcgct gcgccgcacg
     3121 ggcagcggtg cgctctccct cgacggcgcg ctgacgctgg agcaactcgg cgacatgtcc
     3181 gaagcggaac gcgacgccgc gctgcgcgat gccgacgcgt tgctggccga ctggccggtg
     3241 gtgcggctcg gcacggagga tgcgggccgc tttctcaccg gcctgcgccg gcgcaccgat
     3301 ctgcccgacg cggccaacgt tcgcgtctac ggccccgagc cgcgcgcctt tctcggcggc
     3361 ggccacatcg cggccggtga attaatttcg acgaggctgc tgagcccggt ggaggtgcaa
     3421 ggccttctga atccaaccca agctgtgacc acaccatgac ccgccaaatc cgcaacatcg
     3481 ccatcatcgc ccacgtcgac cacggcaaga ccacgctggt cgaccagctg ctgcgccaga
     3541 gcggcacctt ccgcgagaac gagaaggtcg ccgagcgcgt gatggacaac aacgacctcg
     3601 agaaggaacg cggcatcacg atcctgtcga agaactgcgc ggtcgaatgg aagggcacgc
     3661 acgtcaacat cgtggacacc ccgggccacg ccgacttcgg cggtgaagtg gagcgcgtgc
     3721 tgtcgatggt cgactcggtg ctgctgctgg tcgacgcggt cgagggcccg atgccgcaga
     3781 cccgcttcgt gaccaagaag gcgctggcgc tggggctgaa cccgatcgtc gtcgtcaaca
     3841 aggtcgaccg ccccggcgcg cgacccgact acgtgatcaa cgcgaccttc gatctgttcg
     3901 acaagctcgg cgcgaacgag cagcagctcg acttcccggt catcttcgct tcgggcctga
     3961 acggctgggc cacgctgacg cagggcgagg tcggcaccga cctggcgccg ctgttcgaca
     4021 cgatcctgag ccacgtgccg gcgcacgccg gcgagcccga cgcgccgctg cagctgcaga
     4081 tctgctcgct cgactactcg ac
//
LOCUS       DASRDG010000261         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1422419, whole genome shotgun sequence.
ACCESSION   DASRDG010000261 DASRDG010000000
VERSION     DASRDG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422419"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2765
                     /gene="putA"
                     /locus_tag="VFA35_04325"
     CDS             <1..>2765
                     /gene="putA"
                     /locus_tag="VFA35_04325"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HZT55430.1"
                     /translation="EGLGLAIQGYGKRCMAVIDLLAEMAKAQQRRIPTRLVKGAYWDS
                     EIKRAQLDGLDGYPVYTRKAYTDVSYLACARKLLAAPREVYPQFATHNAHTLAAIFEM
                     ADPAAYRPGQYEFQCLHGMGEPLYEQVVGEVELGKLGRPCRIYAPVGTHETLLAYLVR
                     RLLENGANTSFVNRIADPTIPLAELVQNPIEAVEQMAAREGAVGLPHPAIALPVDLCG
                     EGRANSAGLDLANDNCLRDLGDALRISAATQWEASPLLAGDGSPFGDASSWRSVLNPA
                     DPADVVGHVREASTADVDEALRRADDFASTWAGTPPAVRASALEDAADQLELGMPRLI
                     GLLMREAGKTASNAIAEVREAVDFLRFYALQARREFSNDTHQPLGPVVCISPWNFPLA
                     IFVGQVAAALAAGNPVLAKPAEQTPLVAAEAVRALWRAGVPRGALQLLPGRGETVGAR
                     LVGDARTMGVMFTGSTEVARILQRAIAGRVDRCGRAVTLIAETGGQNAMIVDSSALVE
                     QVVGDVVSSAFDSAGQRCSALRVLCVQDEVADRVLEMLHGAMAELRMGDPSVLSTDVG
                     PVIDAEAKANIDQHIDAMRAKGRKVLQRAHPASMPGDGTFVAPTVIELDGFKDLQREI
                     FGPVLHVVRYRRRELDALLEQINGTGYGLTLGLHTRIDETIERVVSMAHAGNVYVNRN
                     MVGAVVGVQPFGGEGLSGTGPKAGGPLYLYRLLSQRPDDALRRALAIAGDEDAPTLAE
                     PTGALQDLQRWAQQTDRPALAQSCKRFAQQSRSGASRVLRGPTGENNLYTLLPREAVL
                     CLADDAADRLVQLAATLAVGSQAVWPIQADALRTALPAAVQERIVLAQDWTAPTVRFD
                     AVLLHGTEQHLQAVCATLAARTGPIIGVQRLAPGASDVKLERLLVERALSVNTAAAGG
                     NASLMTI"
BASE COUNT          419 a          984 c          975 g          387 t
ORIGIN      
        1 gggaagggct gggcctcgcg atccagggtt acggcaaacg ctgcatggcg gtcatcgact
       61 tgctggccga gatggcgaag gcacagcagc ggcgcattcc gacgcgcctg gtcaagggcg
      121 cgtactggga cagcgagatc aagcgtgccc agctcgacgg cctcgacggc tacccggtct
      181 acacccgcaa ggcctacacc gatgtctcgt acctcgcctg cgcgcgcaag ctgctggccg
      241 cgccgcgcga ggtctatccg cagttcgcga cccacaacgc gcacaccttg gcggcgatct
      301 tcgagatggc cgacccggcc gcgtaccgac ccggacagta cgaattccaa tgcctgcacg
      361 gcatgggcga accgctgtac gagcaggtcg tcggcgaggt cgaactcggc aagctggggc
      421 ggccgtgccg catctacgcg ccagtcggca cgcacgagac gctgctcgca tacctggtgc
      481 gccggctgct cgagaacggc gccaacacct cgttcgtcaa ccggatcgcc gaccccacga
      541 tcccgctggc cgaactggtg cagaacccga tcgaagccgt cgaacagatg gcggcgcgcg
      601 agggcgcggt cggcctgccg caccctgcga ttgccctgcc cgtcgacctt tgtggcgaag
      661 gccgcgccaa ctcggccggt ctcgacctcg ccaacgacaa ttgcctgcgc gatctcggcg
      721 acgccttgcg catcagcgcg gccacgcagt gggaagcctc gccgctgctg gccggcgacg
      781 gcagcccgtt cggtgatgcg agttcctggc gatcggtgct gaatccggcc gaccctgccg
      841 atgtcgtcgg ccacgtgagg gaggccagca cggccgacgt ggacgaggcg ctgcgccgcg
      901 ccgacgactt cgcatcgacc tgggcgggca cgcctccggc ggtgcgcgcc tcggcgctcg
      961 aagacgcggc cgaccagctg gagttgggca tgccgcgcct gatcggcctg ttgatgcgcg
     1021 aggccggcaa gaccgcctcg aacgcgatcg ccgaggtgcg cgaggcggtc gacttcctgc
     1081 gcttctacgc gcttcaggcg cgccgcgagt tcagcaacga cacgcaccag ccgctcggcc
     1141 cggtggtctg catcagccct tggaattttc cgctggcgat cttcgtcggc caggtcgccg
     1201 cggcgctggc ggccggcaac ccggtgctcg cgaagccggc cgagcagaca ccgctcgtcg
     1261 ccgccgaggc cgtgcgcgcg ctctggcgtg ccggcgtgcc gcgcggcgca ttgcagctgc
     1321 tgcccgggcg cggcgagacc gtcggcgccc gcctcgtcgg cgacgcccgc acgatgggcg
     1381 tgatgttcac cggctcgacc gaggtggcgc gcattcttca gcgcgccatc gccggccggg
     1441 tcgacagatg cggccgtgcc gtcaccctga ttgcggagac cggcgggcag aacgcgatga
     1501 tcgtcgactc gtcggcgctg gtcgagcagg tggtcggcga cgtcgtgtcc tcggcgttcg
     1561 acagcgccgg ccagcgctgc tccgcgctgc gcgtgctgtg cgttcaggac gaggtcgccg
     1621 accgcgtgct cgagatgctg cacggcgcga tggccgagtt gcgcatgggt gaccccagcg
     1681 tgctgtcgac cgatgtcggc ccggtcatcg acgccgaagc gaaggcgaac atcgaccagc
     1741 acatcgacgc gatgcgcgcc aagggccgca aggtgctgca acgcgcgcac ccggcttcga
     1801 tgcctggcga cggcaccttc gtcgcgccga ccgtgatcga gctcgacggt ttcaaagacc
     1861 tgcagcgcga gatcttcggc ccggtgctgc acgtcgtccg ctaccggcgg cgcgagctcg
     1921 acgcgctgct cgagcagatc aacggcaccg gctacggcct gacgctcggc ctgcacacgc
     1981 ggatcgacga gacgatcgaa cgcgtcgtgt cgatggcgca tgccggcaac gtctacgtca
     2041 accgcaacat ggtcggtgcg gtcgtcggcg tgcagccgtt cggcggcgaa ggtctgtcag
     2101 ggaccggccc caaggccggc gggccgctct acctctaccg cctgctgtcg cagcgaccgg
     2161 acgacgcact gcggcgcgcc ctggcgatcg ccggcgacga ggatgcaccg acgctcgccg
     2221 aaccgaccgg ggccctgcaa gacctgcagc gctgggcgca gcagaccgac cgcccggcgc
     2281 tggcacagag ctgcaagcgc ttcgcacaac agtctcgcag cggcgcgtcc cgtgtgctgc
     2341 gaggcccgac cggcgagaac aacctctaca cgttgctgcc gcgtgaggcc gtgctctgcc
     2401 tcgcggacga cgcggccgac aggttggtgc agctggccgc cacgctcgca gtcggcagcc
     2461 aagcggtctg gccaatccag gcagacgcgc ttcgcacggc gctgccggcc gcggtgcagg
     2521 agcggatcgt gctggcccag gactggaccg cgccgacggt gcgcttcgac gcggtgctgc
     2581 tgcacggcac ggagcagcac ctgcaagccg tctgcgcgac gctggccgcg cgaacgggcc
     2641 cgatcatcgg ggtgcaacgc ctcgcccccg gcgcaagcga tgtgaagctg gagcggctgc
     2701 tcgtcgagcg ggcgctcagc gtcaacacgg cggcggcggg aggcaacgcg agtctgatga
     2761 cgatc
//
LOCUS       DASRDG010000262         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1192043, whole genome shotgun sequence.
ACCESSION   DASRDG010000262 DASRDG010000000
VERSION     DASRDG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192043"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VFA35_04330"
     CDS             <1..1201
                     /locus_tag="VFA35_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HZT55431.1"
                     /translation="FKADRAGNLYTGASTEDTPALVEAAAFRDGIVVAQVHEIVDDVA
                     DLPRVDIPGSWIDFVVQADKPFFIEPLFTRDPRLIKPVHVLMAMMAIRGIYERHQVQS
                     LNHGIGFNTAAIELILPTYGEQLGLKGKICRHWALNPHPSLIPAIESGWVESVHSFGG
                     ELGMERYVAARPDVFFTGADGSMRSNRMLCQLAGQYAVDLFIGSTLQVDGDGHSSTVT
                     RGRLSGFGGAPNMGHQPGGRRHSSDAWLSMLQTHSPLERGRKLVVQMVETFQAGGKPT
                     FVDELDAVQVAKDSGMPLAPVMIYGDDVTHVLTEEGIAYLYKAQSLAQRRAMIAAVAG
                     VTPVGVRHDPAQTQRLRSDGLVALPEDLGVDRNQATRSLLAAKSMADLVEWSGGLYDP
                     PARFRSW"
     gene            1201..>2082
                     /locus_tag="VFA35_04335"
     CDS             1201..>2082
                     /locus_tag="VFA35_04335"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HZT55432.1"
                     /translation="MVAHAPSPAIAPDLPALDTQAAALSQAVVSSLVDEARLSPKPGL
                     VDRRGSGAHDDLSLALMSRSAHSLGPAFMAMARAGQRANAPDVTLRETLAQIGRHAEA
                     TMMAATGGVNTHRGAIWALGLLVAAAAIEPGNTVAAQTASIAAAIAAHPDRFGPSRTG
                     HKGEQACLRFRVAGARGQAQAGFPHVIELGLPELKRSRARGDGETSARLNALLAIMSR
                     LDDTCVLSRGGLAALTQLQDAAAAVLAAGGTATLSGRRALRRLDADALASKLSPGGAA
                     DLLAATLFLDRIEELYEA"
BASE COUNT          323 a          722 c          717 g          320 t
ORIGIN      
        1 cttcaaggcc gaccgcgccg gcaatctgta caccggcgcg agcaccgaag acacgccggc
       61 gctggtcgaa gccgccgcct ttcgcgacgg catcgtggtc gcgcaggtcc acgagatcgt
      121 cgacgatgtc gcggacctgc cgcgggtaga catccccggc tcctggatcg atttcgtggt
      181 gcaggctgac aagcccttct tcatcgaacc gctcttcacg cgcgacccgc gcctgatcaa
      241 gccggtccat gtactgatgg cgatgatggc catccgcggt atctacgagc gccatcaggt
      301 gcagtcgctg aaccacggca tcgggttcaa caccgcggcg atcgagctga tcctgcccac
      361 ctacggcgag cagctcgggc tcaagggcaa gatctgccgc cattgggcgc tcaacccgca
      421 cccgtcgctg atcccggcga tcgagagcgg ctgggtcgag agcgtgcatt ctttcggcgg
      481 cgaactgggg atggagcgat acgtcgccgc gcgacccgac gtcttcttca ctggcgccga
      541 cggctcgatg cgctcgaacc gcatgctctg ccagcttgcg ggccagtatg cggtcgacct
      601 gttcatcggc tcgaccctgc aggtcgacgg cgatggccat tcgtcgacag tgacacgcgg
      661 gcgcctctcc ggcttcggcg gcgcccccaa catgggccac cagcccggcg gccggcgcca
      721 ttcgagcgat gcgtggctca gcatgctgca gacccacagt ccgctggagc gagggcgcaa
      781 gctggtggtg cagatggtcg agaccttcca ggcgggcggc aagccgacct tcgtcgacga
      841 gctcgatgcg gtgcaggtgg cgaaggactc cggcatgccc ctggcgccgg tgatgatcta
      901 cggcgacgat gtgacccacg tgctgaccga agagggcatc gcctacctgt acaaggcgca
      961 gtcgctggca cagcgccgcg cgatgatcgc ggcggtggcc ggcgtcacgc cggtcggcgt
     1021 gcgccatgac ccggcgcaga cgcagcgcct gcgcagtgac gggctagtgg ctctgcccga
     1081 agacctcggc gtggaccgca accaagctac acggtcgttg ctggcagcca agagcatggc
     1141 cgatctggtc gagtggtcgg gtgggttgta cgacccgccg gcacgattca ggagctggtg
     1201 atggtggccc acgccccatc gccggcgatc gcgccggatc tcccagcgct cgacacccag
     1261 gcggctgcgc tcagccaggc ggtggtgtcc agcctggtcg acgaagcccg cctgtcgccc
     1321 aagcccggct tggtggaccg gcgcggaagc ggggcgcacg acgacctgag tctggcgctg
     1381 atgtcgcgct cggcccattc gctgggaccg gccttcatgg ccatggcccg agccggccag
     1441 cgtgccaacg cacccgacgt gactctgcgc gagaccttgg cccagatcgg tcgccacgcc
     1501 gaagccacga tgatggcggc caccggaggc gtgaacacgc accgtggcgc gatctgggcg
     1561 ctgggcttgc tggttgcggc agcggcgatc gaaccgggca ataccgtcgc tgcgcagacc
     1621 gcctcgatcg cggcagccat cgccgctcat cccgaccgtt tcggcccgtc tcgcaccggc
     1681 cacaagggtg agcaggcttg tctgaggttc agggtggcag gcgcacgcgg acaggcgcag
     1741 gctggctttc cgcacgtgat cgagctcggc ctgcccgagc tgaagcgcag ccgcgcacgc
     1801 ggagacggcg agacatcagc gcgcctgaat gcactgctcg cgatcatgag ccgcctcgac
     1861 gacacctgcg tgctgtcgcg cggcgggctc gccgccttga cgcagttgca ggatgcagcc
     1921 gcggcggtgc tcgcggccgg cggcacggcg acgctgtcgg gccggcgtgc gctgcgccga
     1981 ctcgatgccg acgccctcgc atcgaagctg tcccccggcg gggccgccga cctgctcgcc
     2041 gcgactttgt ttctcgaccg catcgaagag ctctacgagg cg
//
LOCUS       DASRDG010000263         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_495024, whole genome shotgun sequence.
ACCESSION   DASRDG010000263 DASRDG010000000
VERSION     DASRDG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495024"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFA35_04340"
     CDS             complement(<1..291)
                     /locus_tag="VFA35_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55433.1"
                     /translation="MGGRRQRWESAAASTRSTSGRPAADGMGVISAALLVVLSSPQGT
                     DAAPPQSSQQAPARAARAAAPDPPPQRLGVEFDPRTGWPRTAVPAPRAAPEAP"
     gene            367..2118
                     /locus_tag="VFA35_04345"
     CDS             367..2118
                     /locus_tag="VFA35_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55434.1"
                     /translation="MRLRTGLLLLLAGCASGAPPRADLERLRAAVPVARELPPGAVVR
                     LLPVSGALPANAPAAAVDGLWREALRQSARLDIAAGPVAAGSAVAGNDPPPALQLAFD
                     ATALTLTATLTAPGTSLHLAAVPLGARWSAALDELAARTRLALGDRLDADPVPVGLAY
                     AEDMTLVAGCERALDGAQDGEFGAAWRQLTVLRPRDGGSPFLLEALASVASLRGQPAD
                     AVEIATEALQMRQRLSPVTQHRLLRTLLLAQASLDAGNARRRDQELLTLATVARRERP
                     YDPEPRLTLGLALNFLGRFDEARAELQALVPRLSRSTTVRYHLGWAELALGDARAASA
                     EFTAIAPLLPQAALIVPRALARYESGDQEGLRSWLQQLIEEHAIASGDALHEVRRMQA
                     AHALLTGRRDEAADRLLQDVGWLLEQPSRLRYRAGEFAETGEVLVRIGHGEGLRPALA
                     TLGRLQTDAALADAVSFVSAMVDTAATRRLAVAAAEGLQQRGATVWGHVLLAYGHEVL
                     GELAAERAALAQAAQQSSSPLVKAALLQNLHRSGDVTGAAALRAALHRELAAIDLRRR
                     SQHPLLSPECALAWLAE"
     gene            complement(2129..>2655)
                     /locus_tag="VFA35_04350"
     CDS             complement(2129..>2655)
                     /locus_tag="VFA35_04350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT55435.1"
                     /translation="LLQSLAQRLNGSPLVHRDQVYHGCMPAQTVARLAHKEARRAQRA
                     DRDRLIAATLELNRADLNVDPRHVDRRWLRDMVDARIADGTTLVVGPPGDPFAKVDLG
                     SRGGFGLVLEGVFTFPEHRGRGLATGLVAAAAGDATMPFVCLHVAADNERARRAYERA
                     GLVETGRCRLLLLG"
BASE COUNT          313 a         1013 c         1025 g          304 t
ORIGIN      
        1 cggcgcctcg ggcgcggcgc gcggcgcagg caccgcagtg cgcggccagc cggtgcgcgg
       61 atcgaactcg acgccgaggc gctgcggcgg cggatccggg gcggcggcgc gggcagcgcg
      121 cgctggcgcc tgctgcgacg actgcggggg cgcagcgtcc gttccctgcg gcgaggagag
      181 cacgaccagc aacgcggcgg agatcacgcc catgccatcg gcagcagggc ggcccgaggt
      241 tgacctggtc gacgccgcgg cgctttccca gcgctgcctg cggccgccca acagccgaat
      301 ctccgcgccg cgcgaggaaa cgcggcagta gtagaacagg ccgcgtcggc gcgcgatgct
      361 tgcgccgtgc gtctccggac cggtctgctg ctccttctcg ccggctgcgc gtcgggcgcg
      421 ccgccgcgcg ccgatctgga gcggctccgc gccgcggtgc cggtggcgcg cgagctgccg
      481 ccgggcgccg tcgtgcgcct gttgcccgtg tcgggggccc tgcccgccaa cgcgccggcc
      541 gccgccgtcg acggactgtg gcgcgaagcg ctgcggcagt cggcgcgcct ggacatcgcg
      601 gcggggccgg tcgcggccgg ctccgcggtc gccggcaacg acccgccgcc cgcgctgcag
      661 ctcgcgttcg acgcgacggc cctcaccctg acggcgacgc tgaccgcgcc gggcacgtcg
      721 ctgcacctcg ccgcggtgcc gctcggcgcg cgctggtcgg ccgcactcga cgaactggcc
      781 gcacgcacgc gcctcgcgct cggcgaccgg ctcgacgccg acccggtccc ggtgggcctc
      841 gcgtatgccg aggacatgac gctcgtcgcc ggctgcgaac gcgcgctcga cggcgcccag
      901 gacggcgagt tcggcgcggc gtggcggcag ctcacggtcc tgcgaccgcg cgacggcggc
      961 agtccgttcc tgctcgaggc gctggcgagc gtcgcgtcgc tgcgcggcca gcccgcggac
     1021 gcggtcgaga tcgcgaccga ggccctgcag atgcgccagc gcctgtcgcc cgtgacgcag
     1081 catcgcctgt tgcgcacgct gctgctcgcg caggcgagcc tcgacgcagg caacgcgcgg
     1141 cggcgcgacc aggaactgct cacgctcgcg acggtcgcgc ggcgcgagcg accgtacgac
     1201 cccgagccgc ggctcacgct cgggctcgcg ctcaacttcc tcggccgttt cgacgaggcg
     1261 cgggccgagc tgcaggcgct cgtaccgcgc ctgtcgagga gcacgaccgt gcgctaccac
     1321 ctcggctggg cagaactcgc gctcggcgac gcgcgcgccg cgagcgccga gttcaccgcg
     1381 atcgcgccac tgctgccgca ggcggcgctg atcgtgccgc gggcgctcgc gcgctacgag
     1441 agcggcgacc aggagggcct gcgcagctgg ctgcagcagc tcatcgagga gcacgcgatc
     1501 gccagcggcg acgccctgca cgaggtgcgc cgcatgcagg ccgcgcacgc gctgctgacg
     1561 gggcggcgcg acgaggcggc cgaccgcctg ctccaggacg tcgggtggct gctcgagcag
     1621 ccgagccgcc tccgctatcg cgccggagag ttcgcggaaa ccggcgaggt gctggtccgg
     1681 atcgggcacg gcgaagggtt gcggcccgcg ctggcgacgc tgggccggct gcagaccgac
     1741 gccgcgctcg ccgacgccgt gtcgttcgtc agcgcgatgg tcgacacggc cgcgacccgc
     1801 cggctcgccg ttgccgccgc cgaaggcctg cagcagcgcg gcgccaccgt gtggggtcac
     1861 gtgctgctcg cgtacgggca cgaggtgctc ggcgaactcg cggcggagcg cgccgcgctc
     1921 gcgcaggcag cgcagcagtc gtcgagcccg ctggtcaagg cggcgctgct gcagaacctg
     1981 caccggagcg gcgacgtcac gggcgccgcc gcgctgcgcg cggcgctgca ccgcgagctc
     2041 gcggcgatcg acctgcggcg ccggagccag catccgctgc tctcgcccga gtgcgcgctc
     2101 gcgtggctcg ccgagtgacc ggcgagaatc agccgagcag cagcagccgg cagcgccccg
     2161 tctcgacgag gccggcgcgt tcgtaggcgc gccgcgcccg ctcgttgtcg gcggccacgt
     2221 gcaggcacac gaacggcatc gtcgcgtccc ccgcagccgc ggccaccagc cccgtcgcga
     2281 gcccgcggcc gcggtgctcg gggaacgtga acacgccctc gagcacgagc ccgaacccgc
     2341 cgcggctgcc gaggtcgacc ttggcgaacg gatcgcccgg cggaccgacg accagcgtcg
     2401 tgccgtcggc gatccgggcg tcgaccatgt cgcgcagcca gcggcggtcg acgtggcgcg
     2461 gatcgacgtt gaggtcggcg cggttgagct cgagcgtcgc ggcgatcaag cggtcgcggt
     2521 ccgcgcgctg cgcgcgccgc gcctccttgt gggcgaggcg cgcgacggtc tgcgccggca
     2581 tgcagccgtg gtagacctga tcgcggtgca cgagcgggct gccgttgagc cgctgcgcga
     2641 gcgactgcag cagcg
//
LOCUS       DASRDG010000264         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1551394, whole genome shotgun sequence.
ACCESSION   DASRDG010000264 DASRDG010000000
VERSION     DASRDG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551394"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFA35_04355"
     CDS             <1..553
                     /locus_tag="VFA35_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HZT55436.1"
                     /translation="TLVANFVAPDIEVWLQSENGMLGIGPFPTEDQVDADLINAGKQT
                     VTTLPGSSIFGSHDSFAMIRGGKINLSILGAMQVSAKGDLANWMIPGKMVKGMGGAMD
                     LVAGVARVLVLMEHVAKKKDGTIDLKIIPECTLPLTGVGVVNRIITDLGVLDVTPAGL
                     KLVELADGVTREELQAKTGVPVL"
     gene            complement(580..1464)
                     /locus_tag="VFA35_04360"
     CDS             complement(580..1464)
                     /locus_tag="VFA35_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55437.1"
                     /translation="MKTSWHRAAVLAVTLAFASVPAPAQDAGALRAKHDSLREKLANN
                     PFQRPLVLESTQSAGDLKGEVYAVVEQPFGVVGPALQDTSHWCDLLILHLNVKGCSAA
                     GKPPTEVLSVVLGRKFDQPLDDGYRVDFAYTMPAASADYLRMQMAADAGPVGTRNYRL
                     ALEAVPLDDRRSFIHMSYAYAYGTTARLAMQAYLSTAGREKVGFSVVGKTEDGKPLYI
                     DGVRGVVERNTMRYYLAIEVYLGSLAAPPGEQQEKRLRDWFAATERHALQLHEIERDD
                     YLKMKRSELQRQKAGALR"
     gene            complement(1489..>2646)
                     /locus_tag="VFA35_04365"
     CDS             complement(1489..>2646)
                     /locus_tag="VFA35_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZT55438.1"
                     /translation="EQAISDSPLLAGNKVQLLQDGPSTYQAMFAAIRAARDHINIESY
                     ILDDDEVGRRFADALIARQGQGVQVNLIRDSVGTLGTPAAFFERLAGAGIQVLEFNPV
                     NPLTAKAGWDVNQRDHRKLLIIDGRTAFLGGINVSSVYSGGSFNQGSKQRPEGKQPWR
                     DTDLQIDGPVVAEFQKLFLETWAKQKGAPLAKRNFFPALTRQGSEVVHAIGSSPDDPY
                     SLMYATLISAIDSAERDVLLTNAYFAPDPQLLASLKAAVARGVDVKLVLPGSSDSALV
                     FNVGRSYYDELLRSGIKIYERQNALLHVKTALIDGVWATVGSTNLDWRSFLHNQEVNA
                     VVLGDDFGQRMRAAFDADLAESKQITLEQWNRRSPIQRIKEQFGRLWQYWL"
BASE COUNT          409 a          874 c          894 g          469 t
ORIGIN      
        1 gacgctggtc gcgaacttcg tcgcgcccga catcgaggtc tggctgcaga gcgagaacgg
       61 catgctcggc atcggcccgt tcccgaccga agaccaggtc gacgccgacc tcatcaacgc
      121 cggcaagcag accgtgacca cgctgccggg ctcgtcgatc ttcggctcgc atgacagctt
      181 tgcgatgatc cgcggcggca agatcaacct gtcgatcctc ggcgcgatgc aggtcagcgc
      241 caagggcgac ctggcgaact ggatgatccc cggcaagatg gtcaagggca tgggtggcgc
      301 gatggacctg gtcgccggcg tcgcgcgcgt gctggtgctg atggagcacg tcgcgaagaa
      361 gaaggacggc acgatcgacc tgaagatcat ccccgagtgc acgctgccgc tgaccggcgt
      421 gggcgtggtc aaccgcatca tcaccgacct cggcgtgctc gacgtgacgc ccgccgggct
      481 gaagctggtc gagctcgccg acggcgtgac gcgcgaggaa ctgcaggcca agaccggggt
      541 gccggtgctc tgaatcctgt gggcctgttc tagcaggcgc tacctgagtg cgccggcctt
      601 ctgccgctgc agttcgctgc gcttcatctt caggtagtcg tcgcgttcga tctcgtgcag
      661 ctgcagcgcg tgacgttcgg tcgccgcgaa ccagtcgcgc aggcgctttt cctgctgctc
      721 gcccggcggc gcggcaagcg agccgagata gacctcgatc gcgaggtagt agcgcatcgt
      781 gttgcgctcg accacgccgc gcacgccatc gatgtacagc ggcttgccgt cctcggtctt
      841 gccgaccacg ctgaagccga ccttctcgcg cccggcggtc gacaagtaag cctgcatcgc
      901 gagccgcgcg gtcgtgccgt aggcataggc atacgacatg tggatgaagc tgcgccggtc
      961 gtcgagcggc accgcctcga gcgcgagccg gtaattgcgc gtgccgaccg ggccggcgtc
     1021 ggcggccatc tgcatgcgca agtagtcggc gctcgccgcc ggcatcgtgt aggcgaagtc
     1081 gacccggtag ccgtcgtcga gcggctggtc gaacttgcgg ccgagcacca cgctcagcac
     1141 ctcggtgggc ggcttgccgg ccgcgctgca acctttcacg ttgaggtgca ggatcagcag
     1201 gtcgcaccag tggctcgtgt cctgcagcgc cgggccgacg acgccgaacg gttgttcgac
     1261 gaccgcgtag acctcgccct tcaggtcacc cgcgctctgc gtcgattcga gcaccagcgg
     1321 gcgctggaac gggttgttcg cgagtttctc gcgcaggctg tcgtgcttgg cgcgcagcgc
     1381 gcctgcgtct tgcgccggcg ccggcaccga agcgaacgcg agagtcacgg cgagcacggc
     1441 ggctcggtgc cacgatgtct tcatgagctt gtctcctcga tcgatcggtc agagccagta
     1501 ctgccaaaga cgcccgaatt gctccttgat gcgctggatc ggcgagcgcc tgttccattg
     1561 ctccagcgtg atctgcttcg actcggccag gtcggcatcg aacgcggccc gcatgcgctg
     1621 gccgaagtca tcgccgagca cgacggcatt gacttcctga ttgtgcagaa agctgcgcca
     1681 gtcgaggttg gtcgaaccca cggttgccca cacgccgtcg atcagcgcgg tcttgacgtg
     1741 cagcagcgcg ttctggcgtt cgtagatctt gatgccgctg cgcagcagtt cgtcgtaata
     1801 ggagcggccg acattgaaca ccagcgccga atcgctgctg cccggcagca ccagtttcac
     1861 gtcgacgccg cgcgcgaccg cggccttcag cgacgcgagc agctgcgggt cgggagcgaa
     1921 gtaggcgttc gtcagcagca catcgcgctc ggccgaatcg atcgccgaga tcagcgttgc
     1981 gtacatcagg ctgtacgggt cgtcgggcga gctgccgatc gcatgcacga cctcgctgcc
     2041 ctggcgcgtc agcgccggga agaagttgcg cttcgccagc ggcgctccct tctgcttggc
     2101 ccaggtttcc aggaacagct tttgaaactc ggcgacgacc gggccgtcga tctgcaggtc
     2161 ggtgtcgcgc cacggctgct tgccttccgg ccgctgcttg gagccctggt tgaacgagcc
     2221 gcccgagtac acgctgctga cgttgatgcc gccgaggaag gcggttctgc cgtcgatgat
     2281 caacagcttg cggtggtcgc gctggttcac gtcccagccc gccttggcgg tgagcgggtt
     2341 caccgggttg aattcgagca cctggatgcc cgcgccggcc aggcgctcga agaacgcggc
     2401 cggcgtgccg agcgtgccca cgctgtcgcg aatcaggttg acctgcacgc cctggccctg
     2461 cctggcgatc agcgcatcgg cgaagcgccg gccgacctcg tcgtcgtcga ggatgtagct
     2521 ctcgatgttg atgtggtcgc gcgccgcgcg gatcgccgcg aacatcgcct ggtaggtgct
     2581 cggcccgtct tgcaacagct gcaccttgtt gccggccagc agcgggctgt cggaaatggc
     2641 ctgctc
//
LOCUS       DASRDG010000265         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1192347, whole genome shotgun sequence.
ACCESSION   DASRDG010000265 DASRDG010000000
VERSION     DASRDG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192347"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VFA35_04370"
     CDS             complement(<1..1296)
                     /locus_tag="VFA35_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55439.1"
                     /translation="MMPNVAPHRARAACLLLSLFAAPLAAQSTHDILQSDYRKARDAA
                     LFDAGQEHLQLGTWARKQGLVPQATTEFLRAVEVSEGKNAGAQQVLGIMRGLGDAFWR
                     GEPKHPAKATVADYGRRAAALELHTRKARIDLAQGAAKAGMPDESREQWLAVLRLGGK
                     LEYDDKDAPRIDGVAVPAEFAGWLQEQTTKIDGRRTFEPGGAKAPRVSGMHETASDLL
                     IVRTDLDEERGKALLALGTALWPALQERLDGAPTRRLAMLVFAHKSDYDAYLDVRGLG
                     VLKAARGAADYGNGQTLICGEGLGDAELSAITLHELSHLFFYGTAPAFLPDWYAEGFA
                     ETFGGQGTFTWDGKALQTGGLLDRGRLDDLRQAPLPLRELLAADAAKLLATDHDKGLR
                     FYAEAWALQRFLRGEKTPWQAKFRQWEAQCRGLVLGAPQG"
     gene            1429..>2051
                     /locus_tag="VFA35_04375"
     CDS             1429..>2051
                     /locus_tag="VFA35_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZT55440.1"
                     /translation="MRIAFVPWCLFVAVLAAQEPGKERLTFADTQKPIGWLSPGPSVA
                     IAWDGKHVDIDEKKAKTWIDPASGKAVDPRPAPAGAPSTSVEGWRARIDDDGDLVLEK
                     TVGAANDRGAGRRRGGRDGAVRAVEGSQQKLAIEGQRTPRRREAHASGDGAFASFVQG
                     NNLYVVDCKTNSVWPVTTDGGPELFHGLLDWVYQEEVYGRGTFKAHW"
BASE COUNT          256 a          753 c          756 g          286 t
ORIGIN      
        1 gccctgcggc gcgccgagca cgagaccgcg gcactgggcc tcccactggc ggaacttcgc
       61 ctgccacggc gtcttctcgc cgcgcaggaa ccgctgcagc gcccacgcct cggcgtagaa
      121 gcgcagcccc ttgtcgtggt cggtcgcgag cagcttcgcg gcgtcggcgg cgagcagctc
      181 gcgcagcggc agcggcgcct gccgcaggtc gtcgaggcgg ccgcggtcga gcagtccgcc
      241 ggtctgcagc gctttgccgt cccaagtgaa cgtgccctgg ccgccgaagg tctccgcgaa
      301 accctcggcg taccagtcgg gcaggaacgc cggcgcggtg ccgtagaaga acaggtgcga
      361 cagttcgtgc agcgtgatcg cggacagctc cgcgtcgccg aggccttcgc cgcagatcag
      421 cgtctggccg ttgccgtagt cggcggcgcc gcgggccgcc ttcagcacgc cgaggccgcg
      481 cacgtcgagg taggcgtcgt agtcgctctt gtgcgcgaac acgagcatcg cgagccggcg
      541 cgtcggcgcg ccgtcgaggc gctcctgcag cgcgggccac agcgccgtgc cgagcgcgag
      601 cagcgccttg ccgcgctcct cgtcgagatc ggtgcgcacg atcagcaggt cgctggcggt
      661 ctcgtgcatg ccgctcacgc gcggcgcctt ggcgccgccc ggctcgaacg tgcgcctgcc
      721 gtcgatcttc gtcgtctgct cctgcagcca gccggcgaac tcggcgggca ccgcgacgcc
      781 gtcgatgcgc ggcgcatcct tgtcgtcgta ctccagcttg ccgccgagcc ggagcaccgc
      841 gagccactgc tcgcggctct cgtcgggcat gcccgccttg gccgcgccct gcgcgaggtc
      901 gatgcgcgcc ttgcgcgtgt gcagctcgag ggccgcggcg cggcggccgt agtcggcgac
      961 cgtcgccttc gcggggtgct tcggctcgcc gcgccagaac gcatcgccga ggccgcgcat
     1021 gatgccgagc acctgctgcg cgccggcgtt cttgccctcg ctcacctcga cggcgcgcag
     1081 gaactccgtc gtcgcctgcg gcacgagtcc ctgcttgcgc gcccacgtgc ccagctgcag
     1141 gtgctcctgg ccggcgtcga acagcgccgc gtcgcgcgcc ttgcggtagt cgctctgcag
     1201 gatgtcgtgc gtgctctggg cggcgagcgg cgccgcgaag agcgacagca gcaggcacgc
     1261 ggctcgcgcg cggtgcggtg cgacgttggg catcacggac ggctctcctc cggtggctcg
     1321 cctcccgggg cgggccggcg tgtgcgggat tctagccgcg gaccgctagg atcgggcgcc
     1381 ctcgccccgg cgctcgccgg tggccatcgc cagccgtaga gaccccgcat gcgaatcgcg
     1441 ttcgttccgt ggtgcctgtt cgtcgccgtc ctcgccgcgc aggagcccgg caaggagcgc
     1501 ctgaccttcg ccgacacgca gaagccgatc ggctggctca gccccgggcc gtcggtcgcc
     1561 atcgcctggg atggcaagca cgtcgacatc gacgagaaga aggcgaagac ctggatcgat
     1621 cctgcgtccg gcaaggccgt cgacccgcgg cccgcccccg cgggcgcgcc atcgacctcg
     1681 gtcgagggct ggcgcgcgcg catcgacgac gacggcgacc tcgtgctgga gaagaccgtc
     1741 ggggcggcga acgaccgcgg tgccggccgg cggcgcggcg gccgcgacgg cgcggtgcgc
     1801 gcggtggaag gcagccagca gaagctcgcg atcgagggcc agcgcacgcc gcgccgccgg
     1861 gaggcgcacg cgagcggcga cggcgcgttc gcgtcgttcg tgcagggcaa caacctctac
     1921 gtcgtcgact gcaagacgaa ctccgtgtgg ccggtgacga ccgacggcgg gcccgagctg
     1981 ttccacggtc tgctcgactg ggtctaccag gaggaggtct acggccgtgg gaccttcaag
     2041 gcgcactggt g
//
LOCUS       DASRDG010000266         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_41380, whole genome shotgun sequence.
ACCESSION   DASRDG010000266 DASRDG010000000
VERSION     DASRDG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41380"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFA35_04380"
     CDS             <1..360
                     /locus_tag="VFA35_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZT55441.1"
                     /translation="FDPQPFHLDDEAAKAALFGALSASGWHTCAMAMRMMCDAYLLES
                     SSLGSPGIDSLRWTQPVRVGDVLSMRMTVLEARPMKSRPGVGLILSTWEVLNQNRETV
                     LTMQGWGMFGRRRPAEA"
     gene            complement(374..1558)
                     /locus_tag="VFA35_04385"
     CDS             complement(374..1558)
                     /locus_tag="VFA35_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZT55442.1"
                     /translation="MPEDPIATFVARLEAALADASFAKLVLAKPRAAGADLVRVTVRP
                     VTLKGEACLSLVHTHKTRDITKNARHADGVQQLRALLETAFGHAHLFTASEEIQLLTG
                     KKGRRTLLRGAASHAAVPAAHDRAKHRFLDLDRPFLTELGVTDAAHRLVPAMARKWKQ
                     INKFVEVLDHALAATALKTAPRLDVIDFGSGKGYLTFAMHDYLRGTLGLPAQVAGVEL
                     RPDMVALCNGAVQRLALDGLRFEQGDVRSVVPEAIDIMIALHACDTATDFALHTGVRA
                     GAQIIMCSPCCHKELRPQLLAPHPLRPVLKHGVHLGQEAEMLTDSLRAMLLDACGYDA
                     QVFEFVSLEHTNKNKMILAVKRTAGRPADEAWRQIDEVKAFYGIRDQCLETLLKADRV
                     AA"
     gene            1578..1916
                     /locus_tag="VFA35_04390"
     CDS             1578..1916
                     /locus_tag="VFA35_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55443.1"
                     /translation="MATRRSRKHHYSVYVVELADTVWNSPAFRNANPEHVLGKPFVYV
                     GMTGLDPDLRFDRHKAGIQANRFVREHGLRLLPQLYAVYNPMPYHGACEMEVELAIAL
                     REQGFGVWQG"
     gene            1961..2506
                     /locus_tag="VFA35_04395"
     CDS             1961..2506
                     /locus_tag="VFA35_04395"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZT55444.1"
                     /translation="MDTGFTLFDTPIGTCALVWGEHGLVGAALPEPSAAAMRAAVRRR
                     FVAAREAAPPPDVQRVIGRVLALLHGATDDLASVALDLRGVPAFNQRVYAVARAIPPG
                     RTLTYGEVAARMGDGPQAARAVGQALGANPFPIIVPCHRVLAAGSRAGGFSAPGGTRT
                     KLRLLEIEGAPLGGSPGLFDT"
     gene            complement(2522..3004)
                     /locus_tag="VFA35_04400"
     CDS             complement(2522..3004)
                     /locus_tag="VFA35_04400"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZT55445.1"
                     /translation="MSFFNPKRLSCGVVILNPDRELLLCHVTGQNHWDLPKGGIDPGE
                     TPLEAAIRETREESGLRLRAECLHDLGRFAYTNKKNLHLFACLLPRFDLKELRCESRY
                     LDRFSGRQLPEMDGFGWFAFERIAALCTPKMSLVLGSRIGLDDALARLTPPQPSAMAA
                     "
     gene            complement(3153..>3334)
                     /locus_tag="VFA35_04405"
     CDS             complement(3153..>3334)
                     /locus_tag="VFA35_04405"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZT55446.1"
                     /translation="VPMQDVLGLPGTHRMNLPGTATGNWSWRFDWDMVGSEPGRVLGR
                     MTAASGRGPFGLLWR"
BASE COUNT          478 a         1184 c         1187 g          485 t
ORIGIN      
        1 ttcgacccgc agccgttcca cttggacgac gaggcagcga aggccgcgct gttcggcgcg
       61 ctgtcggcga gcggctggca cacctgcgcg atggcgatgc gcatgatgtg cgacgcctac
      121 ctgctcgaat cgagcagcct cggctcgccc ggcatcgaca gcctgcgctg gacccagccg
      181 gtgcgcgtcg gcgacgtgct cagcatgcgc atgacggtgc tcgaggcgcg gccgatgaag
      241 agccggcccg gcgtcggact gatcctgtcg acctgggaag tgctgaacca gaaccgcgag
      301 acggtgctga cgatgcaggg ctggggcatg ttcggccgtc gccgaccggc cgaggcctga
      361 aggcgagggc ccgtcacgcc gccacgcggt cggccttcag caacgtctcg aggcactggt
      421 cgcggatgcc gtagaaggcc ttgacctcgt cgatctgccg ccacgcctcg tccgccggcc
      481 ttccggctgt gcgcttcacc gcgaggatca tcttgttctt gttggtgtgc tccagcgaca
      541 cgaactcgaa cacctgcgcg tcgtagccgc aggcgtcgag cagcatcgcg cgcagcgagt
      601 cggtcagcat ctcggcctcc tggccgaggt gcacgccgtg cttcaggacc ggccgcagcg
      661 ggtgcggcgc aagcagctgc ggccgcagct ccttgtggca gcacggcgag cacatgatga
      721 tctgcgcgcc ggcgcgcacg ccggtgtgga gagcaaagtc ggtcgcggtg tcgcaggcgt
      781 gcagcgcgat catgatgtcg atggcttcgg gcacgacgct gcgcacgtcg ccctgctcga
      841 agcgcaggcc gtcgagcgcg aggcgctgca ccgcgccgtt gcacagcgcg accatgtcgg
      901 gccgcagctc gacgccggcc acctgcgcgg gcaggccgag cgtgccgcgc aggtagtcgt
      961 gcatcgcgaa ggtcaggtag cccttgccgc tgccgaagtc gatcacgtcg aggcgcggtg
     1021 cggtcttcag cgcggtcgcg gcgagagcgt ggtcgagcac ctcgacgaac ttgttgatct
     1081 gtttccactt gcgcgccatc gccggcacga gccggtgcgc cgcgtcggtc acgccgagct
     1141 cggtcagaaa cggccggtcg aggtcgagaa agcgatgctt ggcgcggtcg tgcgcggccg
     1201 gcacggcggc gtggctcgcg gcgccgcgca gcagcgtgcg ccggcccttc ttgccggtca
     1261 gcagctgaat ctcctcgctc gcagtgaaca ggtgggcatg gccgaacgcg gtctcgagca
     1321 gcgcgcgcag ctgttgcacg ccgtcggcgt ggcgcgcgtt cttcgtgatg tcgcgcgtct
     1381 tgtgggtgtg caccaacgac aggcaggctt cgcccttcag cgtgaccggc cgcaccgtga
     1441 cgcgcacgag gtcggcgccg gccgcgcgcg gcttggccag cacgagcttc gcgaacgacg
     1501 cgtcggcgag cgcggcttcg aggcgcgcga cgaaggtggc gatggggtct tcgggcatgg
     1561 ctgctagtgt agagagcatg gccacgcgtc gaagccgcaa gcaccactac agcgtctacg
     1621 tcgtcgagct cgccgacacg gtgtggaact cgccagcctt ccgcaacgcg aaccccgaac
     1681 acgtgctcgg caagcccttc gtctacgtcg gcatgaccgg cctcgatccc gacctgcgct
     1741 tcgatcgcca caaggccggc atccaggcga accgcttcgt gcgcgagcac ggcctgcggc
     1801 tgctgcccca gctctacgcg gtctacaacc cgatgccgta ccacggcgcc tgcgagatgg
     1861 aagtcgagct cgcgatcgcg ctgcgcgagc agggcttcgg cgtctggcag ggctaggtgc
     1921 gtgtgagcgc gcccgcgccg tccgcgtcat catcggcggc atggacacgg gcttcacgct
     1981 gttcgacacc ccgatcggca cctgcgcgct ggtctggggc gaacacgggc tggtgggcgc
     2041 ggcactgccg gagccgagcg cggcggcgat gcgggctgcg gtgcggcggc gcttcgtcgc
     2101 ggcgcgcgag gccgcgcccc cgcccgacgt gcaacgcgtg atcggccgcg tgctggcgct
     2161 gctgcatggc gccaccgacg acctcgcgag cgttgcactc gacctgcgcg gcgtgcccgc
     2221 cttcaaccag cgcgtctacg cggtcgcgcg cgcgatcccg cccggccgca cgctgaccta
     2281 cggcgaggtc gccgcccgca tgggcgacgg gccgcaggcc gcgcgcgccg tcggccaggc
     2341 gctgggcgcg aatccgttcc cgatcatcgt gccgtgccac cgcgtgctcg cggccggcag
     2401 ccgcgccggc ggcttctcgg cgccgggcgg cacgcgcacc aagctgcgcc tgctcgagat
     2461 cgaaggggcg ccgctcggcg gctcgccggg gctgttcgac acctgacggc tggaggagcg
     2521 ctcaggccgc catcgcgctc ggctgcggcg gcgtcagccg cgccagcgca tcgtcgaggc
     2581 cgatgcggct gcccagcacc aggctcatct tcggcgtgca cagcgcggcg atgcgctcga
     2641 acgcgaacca gccgaagccg tccatctcgg gcaactgccg gcccgaaaag cggtcgaggt
     2701 agcggctctc gcagcgcagc tccttcaggt cgaagcgcgg cagcaggcag gcgaacaggt
     2761 gcaggttctt cttgttcgtg tacgcgaacc ggcccaggtc gtgcaggcac tcggcccgca
     2821 accgcagccc gctttcctcg cgcgtctcgc ggatcgccgc ttcgagcggc gtctcgccgg
     2881 ggtcgatgcc gcccttgggc agatcccagt ggttctggcc ggtcacgtgg cacagcagca
     2941 gctcgcgatc cgggttcagg atcacgacac cgcaactcag acgcttggga ttgaagaacg
     3001 acatggccac actcgggacg aacgcccaca gtctcgcacg ggcgcgcgcc cgccatcggg
     3061 cgaacagccc gcgaaaaacg tgcgagatcg cgcagcagcg gctcggctga atccctagac
     3121 cgggagccgc tgccgcgcag gtcagcgcga cctcaccgcc acagcaaccc gaacggcccg
     3181 cgcccgctcg ccgccgtcat ccggcccagc acgcggccgg gttcgctgcc gaccatgtcc
     3241 cagtcgaagc gccagctcca gttgccggtt gcggtgccgg gcaggttcat gcggtgcgtg
     3301 ccgggcaggc cgagcacgtc ctgcatcggc acga
//
LOCUS       DASRDG010000267         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_611416, whole genome shotgun sequence.
ACCESSION   DASRDG010000267 DASRDG010000000
VERSION     DASRDG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611416"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..993)
                     /locus_tag="VFA35_04410"
     CDS             complement(124..993)
                     /locus_tag="VFA35_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZT55447.1"
                     /translation="MNTPAPASKRPARALIADDERLMREQLRARLAEVWPELEIVAEA
                     KNGIEAVELTESQRPDLVFLDIRMPGMTGVEAARAIAQLPVAEDAEDAEDGWNGCEIV
                     FITAYDQYAIEAFEQGVVDYVLKPAERERLAVTVERIKKRLAQRDAGGAPSGDDDGDA
                     APAALQQLLQKLAARMDPSRAPQLRWIQATVGQTIQMIPVDEVLFFISDEKYTRVQTA
                     TIEALIRKPIKELIEELDAEVFWQIHRSTLVNTKFIAGVSRDLRGRQLVSVRGHPEKL
                     EVSRSFTGLFKGM"
     gene            complement(990..1403)
                     /locus_tag="VFA35_04415"
     CDS             complement(990..1403)
                     /locus_tag="VFA35_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55448.1"
                     /translation="MKRTLGFLGWALLASLVLGAALVAVAVATLGAADGVTIQLDGAP
                     IGLADLDAGHGLLAVGGVALALLIVALVVPLAVLIPLAMAAVALVGALLVVAGVLAVL
                     CSPLILLALAVWLIVRLARRDNARNRPAASATIAG"
     gene            complement(1400..2848)
                     /locus_tag="VFA35_04420"
     CDS             complement(1400..2848)
                     /locus_tag="VFA35_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZT55449.1"
                     /translation="MSDFATTTASTWKRVSSTAVRLFHQYANWLVGISWWRFGALAIL
                     LLILTAIVQKLPPFRTSWSEIVEDVPTVPIPPVPKAAAPVPPKPPEVKIEAPNPKGGK
                     DEDVVISIDKNGVRITPRAAAVASAASGAEPAAAASGAASAAASGAGSSIEIALPGAT
                     RETIREAVRAAKEAAREAAAEARQQAEEARREAQEAVEEARRDAEEARRDAEEANAQA
                     RHYKRTRVVHLGDSLTDLAFFWIVASAVLKFTYKGRVQADARAAEATETAESEQLKRQ
                     VVEARMAAMQAQVEPHFLFNTLASIDHLIETDPPRASQMQKNLIALLRASMPAMRENH
                     PAAHNLGREMAVIRPYLEILKVRMEDRLQTEISVPDGLLSADFPSMMIQSLVENAIKH
                     GLEPKAEGGKLTVRAEIVHGKLAVTVADTGLGFGKAATAGTGVGLNNIRERLQLLYGH
                     KASMTVAENTPTGTLVTLTVPYQAQTREGMDT"
     gene            complement(3005..3580)
                     /locus_tag="VFA35_04425"
     CDS             complement(3005..3580)
                     /locus_tag="VFA35_04425"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZT55450.1"
                     /translation="MKAFARLGGFALASAAAAQPAPPPPTWTLDPTHTFVHWEVVHMG
                     TSTIRGRFDKVTGSVQFDPKAQQLDLSITVDTASVSSGVTVLDAALKGSAMLDTAANP
                     QAFFVARGASFEGETPREVRGEFTLRGTSQPLSLRALRWHCGLNPLFRRTVCGGDFEA
                     EIVRSSFGITHSLPFVADTVRLVIQVEAISP"
     gene            complement(3577..>3835)
                     /locus_tag="VFA35_04430"
     CDS             complement(3577..>3835)
                     /locus_tag="VFA35_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HZT55451.1"
                     /translation="GTAFDPFGRSAERRTERALITEYQASIDELLGSLNAANLALATE
                     IARIPEDIRGYGHVKERHLASARPKWAALMNEWRAGQQRAA"
BASE COUNT          503 a         1343 c         1378 g          611 t
ORIGIN      
        1 caccgctgtg gatcctgtcg agctactggc tcgcgcaatg ctgcatcgcg gcggcgctgg
       61 ccggacgggc gagcacacgg gacgagcgtt gagatgacgc ccgccgctgc gccgccaccc
      121 gatctacatc cccttgaaca acccggtgaa gctgcggctg acctcgagct tttccggatg
      181 ccccctgacg ctgacgagct ggcggccgcg cagatcgcgg ctgacgccgg caatgaactt
      241 cgtgttgacc agcgtcgagc ggtggatctg ccagaacacc tcggcgtcga gctcctcgat
      301 cagctccttg atcggcttgc ggatcagcgc ttcgatcgtc gccgtctgaa cccgggtgta
      361 cttctcgtcg gagatgaaga acagcacctc gtcgaccggg atcatctgga tcgtctggcc
      421 gacggtcgcc tggatccagc gcagctgcgg cgcgcggctc gggtccatgc gcgcggcgag
      481 cttttgcagc agttgctgca gtgccgcggg cgctgcgtcg ccgtcgtcgt cgccgctcgg
      541 cgcgccgccg gcatcgcgct gcgcgagccg cttcttgatg cgttcgaccg tgacggcgag
      601 ccgctcgcgt tcggcgggct tgagcacgta gtcgaccacg ccctgctcga aggcctcgat
      661 cgcgtactga tcgtaggcgg tgatgaagac gatctcgcaa ccgttccaac cgtcttcggc
      721 gtcttcagcg tcctcggcga ccggcagctg cgcgatcgcg cgcgccgcct cgacgccggt
      781 catgcccggc atgcggatgt cgaggaagac gaggtcgggg cgctgcgatt cggtgagctc
      841 gaccgcctcg atgccgttct tcgcctcggc gacgatctcg agctcgggcc agacctcggc
      901 cagccgcgcg cgcaactgtt cgcgcatcag gcgctcgtcg tcggcgatca gggcgcgggc
      961 agggcgcttc gaagcgggcg caggcgtgtt catccggcga tcgtagcgct ggcggcgggc
     1021 cggttcctcg cgttgtcgcg gcgcgccagg cgcacgatca gccagaccgc cagcgccagc
     1081 aggatcagcg gcgagcacag caccgcgagc acgccggcga cgacgagcaa cgcgcccacc
     1141 agcgccaccg cggccatcgc gagcgggatc agcaccgcca gcggcacgac cagcgcgacg
     1201 atcagcagcg ccagtgccac gccgccgacc gccagcagcc cgtggccggc gtcgagatcg
     1261 gccaggccga tcggcgcgcc gtcgagctgg atcgtcacgc cgtcggcggc gccgagggtt
     1321 gcgaccgcca ccgcgaccag cgcggcgccg agcacgaggc tcgcgagcag ggcccagccg
     1381 aggaagccga gggtgcgttt catgtgtcca ttccttcgcg ggtctgtgcc tggtacggca
     1441 cggtgagggt cacgagcgtg ccggtcggcg tgttctcggc caccgtcatc gaggccttgt
     1501 ggccgtacag cagttgcagg cgctcgcgga tgttgttcag gcccacgccg gtgccggcgg
     1561 tggcggcctt gccgaagccc aggccggtgt cggccaccgt caccgcgagc ttgccgtgca
     1621 cgatctcggc gcgcaccgtg agcttgccgc cctcggcctt gggctcgagg ccgtgcttga
     1681 tcgcgttctc gaccaggctc tggatcatca tcgacgggaa gtcggccgag agcaggccgt
     1741 ccggcacgct gatctcggtc tgcagccggt cttccatgcg caccttcagg atctcgaggt
     1801 agggccggat caccgccatc tcgcggccga ggttgtgcgc cgccgggtgg ttctcgcgca
     1861 tcgccggcat cgaggcgcgc agcagcgcga tcaggttctt ctgcatctgg ctcgcgcgcg
     1921 gcgggtcggt ctcgatcagg tggtcgatgc tcgccagcgt gttgaacaag aagtgcggct
     1981 cgacctgcgc ctgcatcgcc gccatgcgcg cctcgacgac ctggcgtttg agctgctcgg
     2041 actcggccgt ctcggtcgcc tcggcggcgc gcgcgtcggc ctgcacgcgg cccttgtagg
     2101 tgaacttcag caccgccgac gcgacgatcc agaagaacgc gaggtcggtc agcgagtcgc
     2161 ccaggtgcac gacccgcgtg cgcttgtagt ggcgcgcctg ggcgttcgcc tcctcggcgt
     2221 cgcgtcgcgc ctcttcggca tcgcgccgcg cttcctcgac cgcctcctgc gcctcgcggc
     2281 gggcctcctc ggcctgctgg cgcgcttcgg cggcggcttc gcgggccgcc tccttcgcgg
     2341 cgcgcacggc ctcgcggatg gtttcgcggg tcgcgccggg cagcgcgatc tcgatgctgc
     2401 tgcccgcgcc gctggccgcc gcggaagccg cgcccgatgc ggccgcagcc ggttcggcgc
     2461 ccgaggccgc cgacgcgacc gccgccgcgc gcggtgtgat gcgaacgccg ttcttgtcga
     2521 tcgagatcac gacgtcctcg tccttgccgc ccttcgggtt cggcgcctcg atcttgacct
     2581 cggggggctt cggcggcacc ggggccgcgg ccttcggcac cggcgggatc ggcacggtcg
     2641 gcacgtcttc gacgatctcg ctccacgagg tgcggaacgg cggcagcttc tgcacgatgg
     2701 cggtcaggat cagcagcagg atcgcgagcg cgccgaagcg ccaccagctg atgccgacca
     2761 gccagttcgc atactggtga aacagcctga cggccgtgct ggagacgcgc ttccaggtgc
     2821 tggcagtggt ggtggcgaag tcggacatgg aagaggctcg ggcagggcgc ggtttggcga
     2881 tggatggcac tctaagggcg gcgcttgccc ctgcccagcg cgctgcgacg gtttgccgcg
     2941 gcgggccgac agggtgcagc ggccgcggac aggccggttg cgcggcggcc actgacggtc
     3001 atcgctacgg cgagatcgcc tccacctgga tgacgagccg caccgtgtcg gccacgaacg
     3061 gcaggctgtg ggtgatgcca aagctgctgc gcacgatctc ggcctcgaag tcgccgccgc
     3121 agacggtgcg gcggaacagc gggttcaggc cgcagtgcca gcgcagcgcg cgcaagctca
     3181 gcggctggct ggtgccgcgc aaggtgaact cgccgcggac ctcgcgcggc gtctcgccct
     3241 cgaagctcgc gccgcgcgcg acgaagaagg cctgcgggtt cgcggccgtg tcgagcatcg
     3301 cgctgccttt cagcgccgca tcgaggacag tcacgccgct gctgaccgaa gccgtgtcga
     3361 ccgtgatgct gaggtcgagc tgctgcgcct tcgggtcgaa ctgcacgctg ccggtgacct
     3421 tgtcgaagcg gccgcggatc gtcgaggtgc ccatgtgcac gacctcccag tgcacgaagg
     3481 tgtgcgtcgg gtcgagcgtc caggtcggcg gtggcggcgc gggctgcgcc gccgcggcgc
     3541 tggccagcgc gaacccgccg agccgcgcga acgctttcaa gccgcccgct gctggccggc
     3601 gcgccattcg ttcatcagcg cagcccactt cgggcgcgcg ctcgcgaggt gccgctcctt
     3661 cacgtggccg tagccgcgga tgtcttccgg gatgcgcgcg atctcggtag cgagcgccag
     3721 attggcggcg ttcagcgagc cgagcagttc gtcgatcgag gcttggtact cggtgatcag
     3781 cgcgcgctcg gtcctgcgct ccgcgctgcg gccgaacggg tcgaacgcgg tgccg
//
LOCUS       DASRDG010000268         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_965694, whole genome shotgun sequence.
ACCESSION   DASRDG010000268 DASRDG010000000
VERSION     DASRDG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965694"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFA35_04435"
     CDS             <1..621
                     /locus_tag="VFA35_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55452.1"
                     /translation="QNTTLTQYLPGSGLLAGCNGVSLSADGRRLAVTSTAPSLLLIDV
                     RTGALLQNIALPGASNIYTTAWQDLRPGAVYAPYGAGCAGSLGVPTLGAAAPPALGTF
                     FQANVGNLPGGVAFMVTGFSATANGPTPLPLDLGPLGMPGCSLLAEAALTTLLLGGTT
                     ATWQLAIPNDPTYLGFTFYNQGFALDPPANALGLTVSNGAHAVIGI"
     gene            complement(835..910)
                     /locus_tag="VFA35_04440"
     tRNA            complement(835..910)
                     /locus_tag="VFA35_04440"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Lys,seq:ctt)
     gene            1052..1128
                     /locus_tag="VFA35_04445"
     tRNA            1052..1128
                     /locus_tag="VFA35_04445"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1086..1088,aa:His,seq:gtg)
     gene            1267..1476
                     /locus_tag="VFA35_04450"
     CDS             1267..1476
                     /locus_tag="VFA35_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55453.1"
                     /translation="MIYRHDRTLDDAIDLKRAHPMSRLRAALWAAVLLLLLAAYGLRV
                     HQRDESPASTKAGSSMPPASAPAGR"
     gene            complement(1495..>2158)
                     /locus_tag="VFA35_04455"
     CDS             complement(1495..>2158)
                     /locus_tag="VFA35_04455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZT55454.1"
                     /translation="ARAADLGCGTGWFGLRLLRAGLRTVFLDPSGKMLEQARAAAAAE
                     AARGLDATFVQCGLEDMDAVADQSLDFATAQGDPLSFCSDAERALRQLHRALRPDAHV
                     VLSVDNRCAGVRALLDEGAADAALELLRTGRTEWRAQRDAERFAMQMFDAGELGSLLA
                     RCGFAVASCIGKTCLVQRRHEAALADAAARQRWLSAEESVHAQPHWWAAASHLQIAAR
                     RR"
BASE COUNT          290 a          848 c          740 g          280 t
ORIGIN      
        1 cagaacacga cgctcacgca gtacctgccc ggctcgggcc tgctggccgg ctgcaacggc
       61 gtctcgctgt ccgccgacgg ccggcggctc gcggtcacct cgaccgcgcc gtcgctgctg
      121 ctgatcgacg tgcgcaccgg cgcgctgctg cagaacatcg cgctgccggg cgcgagcaac
      181 atctacacga ccgcctggca ggacctgcgg ccgggtgccg tctacgcccc gtacggggca
      241 ggatgcgcgg gctcgctcgg cgtgccgacg ctcggcgccg cagcgccgcc ggcgctcggc
      301 acgttcttcc aggccaacgt cggcaacctg ccgggcggcg tcgccttcat ggtcaccggc
      361 ttctccgcca ccgcgaacgg cccgaccccg ctgccgctcg acctcggtcc cctcggcatg
      421 ccgggctgct cgctgctcgc cgaggccgcc ctcacgacgc tgctgctcgg gggcaccacc
      481 gcgacctggc agctcgcgat cccgaacgat ccgacgtacc tcggcttcac gttctacaac
      541 cagggcttcg cgctcgaccc gcccgcgaac gcgctcggtc tcaccgtcag caacggcgcg
      601 cacgccgtca tcggcatctg atgcaccgcc cgccgtcccg ggccggccgg cggccgccag
      661 tcggtctcca gatgcggacg ccggactgcc gatccgtgcc ccggtgatgg ccccggtgac
      721 cactcctccg cacacgccct gcacgcacct cgcccgcgcg ctgcggcgcg gcgagcacgc
      781 gcgcgagtcc ggccgagaaa gcgctgtttc ccggccgttc tcgcgccttc ggagtggtac
      841 ctccagaagg gctcgaacct tcgacccgcg gattaagaat ccgctgctct accaactgag
      901 ctatggaggc gcgcttgggg gcgcgaaagc tagcgacgcc ccgacgcgcg cgcaagcgcg
      961 cgcgggccac tttcgcgccg cgaccttgac ccctgctggc gacagcggca cagtgctccg
     1021 cccggatcgt gccgcacggc ccggacgcac ggtggctgta gctcagttgg ttagagcatc
     1081 aggttgtggt cctgagggtc gcgggttcga atcccgtcag ccaccccagt caataggggc
     1141 ccagtcgaca ggtgcccagt cgacaggggc gcagtcgaga gcggcacagt cgagagggga
     1201 actgtcaccc gcgccaggca cgacccgcgg ggctggcgga ccggcacgga gcgcacggag
     1261 gccgcagtga tctaccgtca cgaccgcacg ctcgacgacg ccatcgacct caagcgcgcg
     1321 caccccatgt cgcgcctgcg cgcggccctg tgggcagcgg tgctgctgct gctgctcgcc
     1381 gcctacggcc tgcgcgtgca ccagcgcgac gagagtcccg cgtcgaccaa ggccggcagc
     1441 tcgatgccgc ccgcgagcgc gccggctggc cgctgaatcg cgcacacggg cgcgtcagcg
     1501 gcgcctggcg gcgatctgca ggtgcgacgc ggcggcccac cagtgcggct gcgcatgcac
     1561 ggactcctcg gccgacagcc agcgctgccg cgccgctgcg tcggccagcg ctgcctcgtg
     1621 ccgtcgctgc acgagacagg tcttgccgat gcagctcgcc accgcgaagc cgcaccgcgc
     1681 gagcagcgac ccgagctcgc cggcgtcgaa catctgcatc gcgaagcgct ccgcatcgcg
     1741 ctgcgcgcgc cactcggtgc ggccggtgcg cagcagttcc agcgccgcgt cggccgcgcc
     1801 ctcgtcgagc agcgcgcgca cgccggcgca gcggttgtcg accgacagca cgacgtgcgc
     1861 gtccggccgc agtgcgcggt gcagctggcg cagcgcgcgc tcggcgtcgc tgcagaacga
     1921 cagcggatcg ccctgtgccg tggcgaagtc gaggctctgg tcggcgactg cgtccatgtc
     1981 ctcgaggccg cactgcacga acgtcgcgtc gagcccgcgc gccgcttcgg ctgccgccgc
     2041 ggcgcgcgcc tgctcgagca tcttgccgct cgggtcgagg aacaccgtgc gcagacccgc
     2101 gcgcagcagc cgcaggccga accagccggt gccgcagccg aggtcggccg cgcgcgcc
//
LOCUS       DASRDG010000269         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_845306, whole genome shotgun sequence.
ACCESSION   DASRDG010000269 DASRDG010000000
VERSION     DASRDG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845306"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFA35_04460"
     CDS             <1..541
                     /locus_tag="VFA35_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZT55455.1"
                     /translation="ALIICHDGMFPEMARECAYKGAEIMIRTAGYTAPIRESWRFTNQ
                     ANAFCNLAYTASVCMCGSDGSFDSMGEGMFCDIDGTILVEGGGRADEIITCELRPDLV
                     REARSGWGVENNIYQLYHRGYVAVRGGAMDCPYTFMQDMVAGTFRLPWEADVVHTDGS
                     SCGFEPPTRLHAPRNRLPE"
     gene            562..1254
                     /locus_tag="VFA35_04465"
     CDS             562..1254
                     /locus_tag="VFA35_04465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HZT55456.1"
                     /translation="MSTTFIDANPYPWPYNGDLRPDNTALVVIDMQTDFCGVGGYVDK
                     MGYDLSLTRAPIAPIKTLMTRLRTLGFTIIHTREGHRPDLSDLPANKRWRSRRIGTNG
                     VGIGDAGPCGRILVRGEPGWQIIDELAPLPGEIVIDKPGKGSFCATDLELILRTRGIE
                     NLLLAGITTDVCVHTTMREANDRGFECLLLDDCTAATDRGNHLAALKMIEMQGGVFGA
                     HAMSSAVLEGLV"
     gene            1381..2979
                     /locus_tag="VFA35_04470"
     CDS             1381..2979
                     /locus_tag="VFA35_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT55457.1"
                     /translation="MTAPLDPKPTATGALTLETFDLTKRFGSFTAMDAVSMRVEAGSV
                     HALLGENGAGKSTLVKCVAGSQRPEGGSIMVDGREHDIATPVIARALGIGMVYQHFTL
                     APGMNVAENLLLAGGRTPALIDWKKERTALQAFLETTPFKLDLDATPAGLAAGEKQKL
                     ELLKQLYLKPRLLILDEPTSVLTPQEADEVLAYVRAVAKRGECTVLMITHKFREVMAY
                     ADEVTVLRRGKRVHGAHVAGTTPEQLAQAMIGDSAASASAIVAEGSAAPRAATPLDAA
                     IALQVDGLSAMGDRGTLAVQQLSLTVKKGEILGIAGVSGNGQRELVEALVGQRPRTAG
                     TVQVVGRPYLAKRAENRVLQLRALPEEPLRNACVGELSVAQNMALRDFDEAPLKVGWL
                     RFGVWRERARAWIAEYGVKTRGENAPIASLSGGNVQRAVLARELAGSINVLIAANPVF
                     GLDFAAVAEIHARILQVRAKGGAVLLISEDLDELLELSDRIAVMSEGRIVFETPAAGA
                     DRRVIGAHMGGGHHDEAHALPEAA"
     gene            2976..>3119
                     /locus_tag="VFA35_04475"
     CDS             2976..>3119
                     /locus_tag="VFA35_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HZT55458.1"
                     /translation="MSGSLIEVPATPFPYPFATGGKTALVVIDMQRDFVEPGGFGASL
                     GNDV"
BASE COUNT          517 a         1102 c         1065 g          435 t
ORIGIN      
        1 cgcgctgatc atctgccatg acggtatgtt tccggagatg gcgcgcgagt gcgcctacaa
       61 gggcgccgag atcatgatcc gcaccgccgg ctacaccgcg ccgatccgcg aaagctggcg
      121 cttcaccaac caggccaacg ccttctgcaa cctggcctat accgccagcg tgtgcatgtg
      181 cggcagcgac ggcagcttcg actcgatggg cgagggcatg ttctgcgaca tcgacggcac
      241 gatcctcgtc gaaggcggcg gccgcgccga cgagatcatc acctgcgagc tgcgccccga
      301 cctcgttcgc gaagcgcgca gcggctgggg cgtcgagaac aacatctacc agctctatca
      361 ccgcggctat gtggcggtga ggggcggcgc gatggactgc ccgtacacct tcatgcagga
      421 catggtggcc ggcacgttcc gcctgccgtg ggaagccgat gtcgtgcaca ccgacggcag
      481 ctcgtgcggc ttcgagccgc cgacccgcct gcatgcgccg cgcaaccggc tgcccgagtg
      541 agccccagga gcgagcaccc cgtgagcacg accttcatcg acgccaaccc ctacccctgg
      601 ccctacaacg gcgacctgcg cccggacaat accgcgctgg tcgtgatcga catgcagacc
      661 gacttctgcg gcgtcggcgg ctatgtcgac aagatgggct acgacctctc gctgacgcgc
      721 gcaccgatcg cgccgatcaa gacattgatg acgcggctgc gcacgctcgg cttcacgatc
      781 atccacaccc gcgaagggca tcggcccgac ctgtcggacc tgcccgcgaa caagcgctgg
      841 cgctcgcggc ggatcggcac caacggcgtc ggcatcggcg acgccgggcc gtgcggccgc
      901 atcctggtgc gcggcgagcc gggctggcag atcatcgacg agctcgcgcc gctgccgggc
      961 gagatcgtca tcgacaagcc cggcaagggc tcgttctgcg cgaccgacct ggaactgatc
     1021 ctgcgcaccc gcggcatcga gaacctgctg ctggccggca tcacgaccga cgtgtgcgtg
     1081 cacaccacca tgcgcgaggc gaacgaccgc ggcttcgagt gcctgctgct cgacgactgc
     1141 accgccgcga ccgaccgcgg caaccacctc gcggcgctga agatgatcga gatgcagggc
     1201 ggggtgttcg gcgcgcatgc gatgtcttcg gcggtgttgg aggggttggt gtgaacggga
     1261 cgcctctttc tgaccgcaaa gaacgcaaag aaagaaaagg ctgtcttctt cttggcgctc
     1321 tttgcgtcct ttgcgttctt tgcggtcaat aaaagcgtgg cacgccaccg gagcccccac
     1381 atgaccgccc ccctcgaccc caagcccacc gccaccggcg ccctcacgct cgaaaccttc
     1441 gacctcacca agcgcttcgg cagcttcacc gcgatggacg ccgtgagcat gcgggtcgag
     1501 gccggctcgg tgcacgcgct gctcggcgag aacggcgccg gcaagagcac gctggtgaag
     1561 tgcgtcgccg gctcgcagcg gcccgagggc ggcagcatca tggtcgacgg ccgcgagcac
     1621 gacatcgcca cgccggtgat cgcgcgcgcg ctcggcatcg gcatggtcta ccagcacttc
     1681 acgctcgcgc cgggcatgaa cgtcgccgaa aacctgctgc tcgccggcgg ccgcacgccg
     1741 gcgctgatcg actggaagaa ggagcgcacc gcgctgcagg cgtttctgga aacgaccccg
     1801 ttcaagctcg acctcgacgc cacacccgcg ggcctcgcgg ccggcgagaa gcagaagctc
     1861 gaactgctga agcagctcta cctgaaaccg cgcctgctga tcctcgacga gccgacctcg
     1921 gtgctgacgc cgcaggaggc cgacgaggtg ctcgcctacg tgcgcgcggt cgcaaagcgc
     1981 ggcgagtgca ccgtactgat gatcacgcac aagttccgcg aggtgatggc ctatgccgac
     2041 gaggtgacgg tgctgcgccg cggcaagcgc gtgcacggcg cgcacgtggc gggcaccacg
     2101 cccgagcaac tcgcgcaggc gatgatcggc gactcggccg cgagcgcgtc ggcgatcgtc
     2161 gccgaaggca gcgcggcgcc gcgcgccgcg acgccgcttg acgccgcgat cgcgctgcag
     2221 gtcgacggcc tgtcggcgat gggcgaccgc ggcacgctcg cggtgcagca gctgagcctg
     2281 acggtgaaga agggcgagat cctcggcatc gccggcgtct cgggcaacgg ccagcgcgag
     2341 ctggtcgagg cgctggtcgg gcaacggccg cgcaccgccg gcaccgtgca ggtcgtcggc
     2401 cggccttatc tcgcgaagcg cgccgagaac cgcgtgctgc aactgcgcgc gctgcccgag
     2461 gagccgctgc gcaacgcctg cgtcggcgag ctcagcgtcg cgcagaacat ggcgctgcgt
     2521 gacttcgacg aagcgccgct caaagtcggc tggcttcgct tcggcgtgtg gcgcgaacgc
     2581 gcgcgcgcct ggatcgccga gtacggcgtg aagacgcgcg gcgagaacgc gccgatcgcc
     2641 agcctctcgg gcggcaacgt gcagcgcgcg gtgctcgcgc gcgagctcgc cggcagcatc
     2701 aacgtgctga tcgccgcgaa cccggtgttc ggcctggact tcgcggccgt cgccgagatc
     2761 cacgcgcgca tcctgcaggt gcgcgcgaag ggcggcgcgg tgctgctgat cagcgaagac
     2821 ctcgacgagc tgctcgaact ctcggaccgc atcgcggtga tgagcgaagg ccgcatcgtg
     2881 ttcgagacgc cggccgccgg cgccgaccgg cgcgtgatcg gcgcgcacat gggcggcggg
     2941 catcacgacg aagcacacgc gctgccggag gctgcatgag cggcagcttg atcgaggtcc
     3001 cggcgacgcc cttcccgtac ccgttcgcga ccggcggcaa gaccgccctg gtcgtgatcg
     3061 acatgcagcg cgacttcgtc gaacccggcg gcttcggcgc ctcgctcggc aacgacgtg
//
LOCUS       DASRDG010000270         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_401458, whole genome shotgun sequence.
ACCESSION   DASRDG010000270 DASRDG010000000
VERSION     DASRDG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401458"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFA35_04480"
     CDS             complement(<1..209)
                     /locus_tag="VFA35_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55459.1"
                     /translation="MTPNPNTPSKPVYGRTLFGAVAAVLALCLALIAAAPPAHAQAGD
                     QTIVDAREAFRKKDRARLAALRSVA"
     gene            222..803
                     /locus_tag="VFA35_04485"
     CDS             222..803
                     /locus_tag="VFA35_04485"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZT55460.1"
                     /translation="MNSASHGAPSGPRQALRTRLLAQREQFVAGDGFEAAFAALARHL
                     TQVLSELEPQSLGLYWPHRSEFNAVGALLADAGFAKLPLALPFAQRAPVQMHYRAWDR
                     QPPAAVDECGIATSAGAPVVPDVALVPCVGFTAAGYRLGYGGGYFDRWLAEHPHVTAV
                     GVAWAIAEVDAASFAPAPHDQPLTLIVTERGVV"
     gene            complement(813..1775)
                     /locus_tag="VFA35_04490"
     CDS             complement(813..1775)
                     /locus_tag="VFA35_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZT55461.1"
                     /translation="MSGSSRRPLSLGNLRAFEAVARRLSFSEAAEELFVTQSAISRQI
                     KGLEDELGAALFVRGTRHVEMTQSGQLLLRAVEAGLPKIDASVRQIRAARSRKRVSVT
                     TFASFGSLWLLPRIEAFQREHPDIDIRVSAADAIADLDDPELDLALRYCSPAQAPAGA
                     TRLFGEVLTPVVSRSLAEQIRVGKAPPLSKPADLAQHTLAEEDDTKTSTEYLSWRRWL
                     TLQGQPGLQPKRWLYLNFTYQQVQAALAGHGVALARVALVFEALQRGELVEPFGPAGR
                     MDSPYLYWLVVAPASRTRPEVVQFCAWVLAQAADTRRAVGEGEG"
     gene            complement(1807..>2014)
                     /locus_tag="VFA35_04495"
     CDS             complement(1807..>2014)
                     /locus_tag="VFA35_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55462.1"
                     /translation="DEVVAEVVGARQPDGAVEHGQFTAQAPEVSGVGPELEGSAMKQV
                     ELRIHHQSPAFGIAMISHITIGIQ"
BASE COUNT          285 a          710 c          702 g          317 t
ORIGIN      
        1 atcgcgacgc tgcgcagcgc ggccagccgg gcgcggtcct tcttgcggaa ggcctcgcgc
       61 gcgtcgacga tcgtctggtc gccggcctgc gcgtgcgcgg gcggcgcggc cgcgatcagg
      121 gccaggcaca gcgcaagcac ggcggcgacc gcaccgaaca aggtgcgccc atatactggt
      181 ttggagggtg tgttcgggtt cggcgtcatc acgggatcat cttgaattca gcatcacacg
      241 gcgcgccgtc ggggccgcgg caggcactgc gcacgcgctt gctggcgcag cgcgagcagt
      301 tcgtcgccgg cgacggcttc gaggcggcct ttgctgcgct cgcgcggcat ctgacccagg
      361 tgttatcgga gctggagccg caatctttgg gtctgtattg gccgcatcgg tccgaattta
      421 acgcagtggg cgcgctcctc gccgatgccg gcttcgcaaa gttgccactc gcgctgccat
      481 ttgcacagcg cgcgccggtg cagatgcact accgcgcgtg ggaccggcag ccgccggccg
      541 ccgtcgacga gtgcggcatc gcgaccagcg ccggcgcacc ggtcgttccc gacgtggcgc
      601 tggtgccgtg cgtgggcttc acggcggcgg gctaccggct cggctacggc ggcggctact
      661 tcgaccgctg gcttgccgag cacccgcacg tgacggcggt cggcgtggcc tgggccatcg
      721 ccgaggtcga cgcggcgtcg ttcgcgcctg caccgcacga ccagccactg acgctgatcg
      781 tcaccgagcg cggcgtggtc tgacaggctt ttctagccct caccctcgcc gaccgccctg
      841 cgcgtgtcgg ccgcctgcgc cagcacccag gcgcagaact gcaccacctc gggccgcgtg
      901 cggctcgcgg gcgcgacgac gagccagtac aggtacgggc tgtccatgcg cccggcaggg
      961 ccgaacggct cgaccagttc gccacgctgc aaggcctcga acacgagcgc gacgcgcgcc
     1021 agcgcaaccc cgtggccggc cagcgccgcc tgcacctgct ggtaggtgaa gttcaggtac
     1081 agccagcgtt tcggctggag cccgggctgg ccctgcagcg tcagccagcg gcgccagctc
     1141 aggtactcgg tgctggtctt ggtgtcgtct tcttcggcga gcgtgtgctg cgccagatcg
     1201 gccggcttgg acaagggcgg cgccttgccc acgcggatct gctcggccag cgagcggctc
     1261 acgaccggcg tgagcacctc gccgaacagc cgggtcgcac cggccggcgc ctgcgccggg
     1321 ctgcagtagc gcagcgcgag gtcgagctcg gggtcgtcca ggtcggcgat cgcgtccgcg
     1381 gccgagacgc ggatgtcgat gtcggggtgc tcgcgctgga acgcctcgat gcgcggcagc
     1441 agccagagcg agccgaacga cgcgaaggtg gtcacgctga cgcgcttgcg gctgcgcgcc
     1501 gcgcggatct gccgcacgct cgcgtcgatc ttcggcaggc cggcctcgac cgcgcgcagc
     1561 agcagttggc cgctctgcgt catctcgacg tggcgcgtgc cgcgcacgaa caacgccgcg
     1621 ccgagttcgt cttcgagccc cttgatctgc cggctgatcg ccgactgcgt gacgaacagc
     1681 tcctcggccg cctcgctgaa gctcaggcgc cgcgcgaccg cctcgaaggc gcgcagattg
     1741 cccagcgaga gcgggcgccg cgagctgcca ctcatatcaa atccgcatca atggccgacc
     1801 tgagtttcat tggattccaa tggtgatatg gctgatcata gcgataccaa acgcaggaga
     1861 ttgatgatga atgcgcaatt ccacctgttt catggcggaa ccttccagct ccggtccgac
     1921 gccgctcacc tcgggcgcct gggcggtgaa ctgaccgtgc tcgacggccc cgtctggctg
     1981 acgcgcgcca acgacctcgg cgaccacttc gtcg
//
LOCUS       DASRDG010000271         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1074057, whole genome shotgun sequence.
ACCESSION   DASRDG010000271 DASRDG010000000
VERSION     DASRDG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074057"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VFA35_04500"
     CDS             complement(<1..1019)
                     /locus_tag="VFA35_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HZT55463.1"
                     /translation="MCLAILVAAGLAACAAAPVTAAPSPPAAAGDAAADDAAVQRGFA
                     RWVANYRVDARAAGIDEATLGAAFAEVHYLARVVELDRAQPEFSRTAWDYLDAAVSPQ
                     RVALGQDKLLQFRAEADAAAARYGVPAALVVAIWGIESNYGANHGSIPTIDALATLGF
                     DGRREAWARGQLLAALKIIANGDIARAQMIGSWAGAMGQTQFLPSVFLAYAVDADGDG
                     RRDIWGSLADVMASTANFLKASGWQAGQPWGVEVELPPGFDLGRADDALRQSGALWAA
                     EGVRTADGAALPELGDAAILLPAGARGPAFLVGRNFRAILRYNNSTSYALAVSLLAQR
                     IAGGPG"
     gene            1215..2054
                     /locus_tag="VFA35_04505"
     CDS             1215..2054
                     /locus_tag="VFA35_04505"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HZT55464.1"
                     /translation="MNADDRSRHALNTALTRLLRPLFRLLLRYGVSYGSFEELARQTF
                     VDVAMDEAGIDGKKPSISRISVLSGLTRKEVQRLSTSVAVGPAADQHNRAARVLTGWV
                     RDADFHDARGQPRVLDYDGEGGFSLLVKRHSGDVPTRAVLDELVRVGAVGLRPDGRIE
                     LIVRAYVPSQSTVEKLRILGNDVADMIDTIDHNLVHGATDPRFQRKVMYRSMPAGALP
                     GFRKLSAKQSQLLLEKMDRWLAAHDLDNPPDQPDLPRARVGLGVYYFEEPAAAQRPQG
                     EDS"
     gene            2051..>2546
                     /locus_tag="VFA35_04510"
     CDS             2051..>2546
                     /locus_tag="VFA35_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HZT55465.1"
                     /translation="MRTTRFAAALAAMAMSFGLAGCGGGGGIEGTGSAQGTMRMSMTD
                     APACGFDAVHVTVDRVRVHQSSTAADADAGWSEVVLNPVKRIDLLTLSNGVLEPLGQT
                     DLPAGKYTQLRLVLASNTAAAPLANSVTPTGGVETELTTPSGQQSGLKLNVNIDIPVG
                     KVADF"
BASE COUNT          387 a          919 c          896 g          344 t
ORIGIN      
        1 cccgggccgc cggcgatgcg ctgggccagc aggctcaccg ccagcgcgta gctggtggag
       61 ttgttgtagc gcaggatcgc gcggaagttg cgtccgacca ggaacgccgg cccgcgcgca
      121 ccggcgggca gcaggatcgc ggcgtccccc agttccggca gcgcggcgcc gtcggccgtg
      181 cgcacgcctt cggcggccca tagcgcgcca ctctggcgca gcgcgtcgtc ggcgcggccg
      241 aggtcgaagc cgggcggcag ctcgacctcc acgccccagg gctgccccgc ctgccagccc
      301 gacgccttca ggaagttcgc ggtcgaggcc atcacgtcgg ccaggctgcc ccagatgtcg
      361 cgccgaccgt cgccgtcggc atccaccgcg taggcgagga acaccgacgg caggaactgc
      421 gtctgcccca tcgcgccggc ccacgagccg atcatctgcg cgcgcgcgat gtcgccgttg
      481 gcgatgatct tcagcgccgc gagcaactgg ccgcgcgccc aggcctcgcg ccgcccgtcg
      541 aagcccagcg tcgccagcgc gtcgatcgtc gggatgctgc cgtggttggc gccgtagttg
      601 ctctcgatgc cccagatcgc gaccacgagc gcggccggca cgccgtagcg cgcggccgcc
      661 gcgtcggcct cggcgcggaa ctgcagcagc ttgtcctgcc ccagcgcgac gcgctgcggc
      721 gacacggcgg cgtcgaggta gtcccaggcc gtgcggctga attcgggctg cgcccgatcg
      781 agttcgacga cgcgcgccag gtagtgaacc tcggcgaacg ccgcgccgag cgtggcctcg
      841 tcgatgccgg cggcgcgcgc atcgacgcga tagttcgcga cccagcgggc gaagccccgt
      901 tgaacggccg cgtcgtcggc cgcggcgtcg ccggcggcgg caggcggcga cggtgcggcg
      961 gtcaccggcg cggccgcgca ggcggccaga cccgccgcca ccaggatcgc caggcacatc
     1021 cgcatgagcc cgccgggccg ccccaagggc gaattccgga gtgcgcggca cgaaggtact
     1081 ccaatgagtg cgcgcgacag cgcaagcgtg gatgattcag gcatgcgccc gattctgaac
     1141 gctgtcccgg tcggccaggg ggtaacgtct ggtgacttga caagccccca tgctgggaat
     1201 aatttcccaa ctccatgaac gccgacgatc gctcgcgcca cgcgctgaac accgcactca
     1261 cgcggctgct gcggccgttg ttccggctgc tgctgcgcta cggcgtgtcg tatggctcgt
     1321 tcgaggagct cgcgcgccag accttcgtcg atgtcgcgat ggacgaggcc ggcatcgacg
     1381 gcaagaagcc gtcgatctcg cgcatctcgg tcctgtcggg cctgacgcgc aaggaggtgc
     1441 agcgcctgtc cacgtcggtc gcggtcgggc ctgctgccga ccagcacaac cgcgcggcac
     1501 gggtgctgac cggctgggtg cgcgacgccg actttcacga cgcgcgcggc cagccccgcg
     1561 tgctcgacta cgacggcgag ggcggcttct cgctgctggt gaagcgccac agcggcgacg
     1621 tgccgacgcg cgccgtcctc gacgaactgg tccgtgtcgg cgcggtcggc ctgcgccccg
     1681 acggccggat cgaattgata gtgcgcgcct acgtgccgag ccagagcacg gtcgagaaac
     1741 tgcgcatcct gggcaacgac gtggccgaca tgatcgacac gatcgatcac aacctggtgc
     1801 acggcgcgac cgacccgcgt ttccagcgca aggtgatgta ccgcagcatg ccggccggcg
     1861 cgctgccagg ctttcgcaag ctcagcgcga agcagtcgca gttgctgctc gagaagatgg
     1921 accgctggct cgccgcgcac gacctcgaca acccgcccga ccagcccgac ctgccacgcg
     1981 cacgcgtcgg tctgggcgtc tactatttcg aagagccggc cgccgcccag cgaccgcaag
     2041 gagaagactc atgagaacca cccgatttgc cgcggcgctc gccgcgatgg ccatgtcgtt
     2101 cgggctcgcc ggctgcggcg gcggcggcgg catcgaaggc accggctcgg cgcagggcac
     2161 gatgcggatg tcgatgaccg acgcgcccgc gtgcggcttc gatgccgttc acgtcacggt
     2221 cgaccgggtg cgcgtgcacc agagcagcac cgcagcggat gccgacgcag gctggtcgga
     2281 ggtggtgctg aacccggtca agcggatcga tctgctgacg ctgtcgaacg gcgtgctcga
     2341 gccgctcggc cagaccgatc tgccggccgg caagtacacg caactgcggc tggtgcttgc
     2401 gtcgaacacg gccgcggccc cgcttgcgaa ctcggtcacg cccaccggcg gcgtcgagac
     2461 cgagctgacc acgccgagcg gtcagcaaag cgggctgaag ctgaacgtga acatcgacat
     2521 cccggtcggc aaggtcgccg acttcg
//
LOCUS       DASRDG010000272         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_966673, whole genome shotgun sequence.
ACCESSION   DASRDG010000272 DASRDG010000000
VERSION     DASRDG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966673"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..859)
                     /locus_tag="VFA35_04515"
     CDS             complement(155..859)
                     /locus_tag="VFA35_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZT55466.1"
                     /translation="MNTPVLTIAERSNIDAGSWFSRLSPPLRHAILSRAAVRRLSDGT
                     MLSARGAPAEEWCGVAKGAVRISSVSLSGKQITLTYVEPGTWFGDISLFDGLPRTHDS
                     NAHGETTLLVVRKADFRELLAQHSELYEALLRLNCRRLRLMFDAVEDLNTRPLASRLA
                     KQILLLARSYGIAQGEEIRIGLQLAQEDLAQLLGASRQRVNQELKGFERDGAVRIEPT
                     RLVVLSKEKLLAIAGK"
     gene            959..1738
                     /locus_tag="VFA35_04520"
     CDS             959..1738
                     /locus_tag="VFA35_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZT55467.1"
                     /translation="MNPPTLDISSRKGQVSAEEWQTRVDLAAAYRLVAMFKWDDLVFT
                     HISARVPGRDDAFLINPYGLMFDEITASSLIKVDAQGHKLDASEYDVNPAGFTIHSAI
                     HAARHDAQCVLHVHTLNGVAVSAQKGGVLPISQQSIFVLSGLSYHGYEGVALRDDEKP
                     RLVADLGPENNFLMLRNHGLLTLGATIADAFLNMYLFETVCTIQVRAMAGGTELIPVD
                     ARIIAGAQQQAKQVTRGVGGGALAWPGLLRRLDRADPSYRT"
     gene            1947..>2745
                     /locus_tag="VFA35_04525"
     CDS             1947..>2745
                     /locus_tag="VFA35_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55468.1"
                     /translation="MRAVSTLTASCAIGTALWLAASSADAVGFGRVTNTTQLGQPLNF
                     VAAVRLDPDELLPRECVSAEVQSGDNRLQPQQIRVTLEGAADAADRNVRVTTSALIDE
                     PVVTVSVTLGCTSKVTRRFVAFIDPPLINPAQTAPAEALPPQRMDSQVAPIVTMVQGA
                     DGSAAVARHADRGGTAPRSRPAARVGASAGSGVARAPAAPRAEAPKRAAPASRQPIVA
                     RAAPAGARLQLEAAAPVSARSASAAASAPIAVNSAAPAPINVTAASAA"
BASE COUNT          409 a          991 c          944 g          401 t
ORIGIN      
        1 tttcgaggta agactgcagc gcgccggtgt cgaaggcgtg cgagtcggcg acgggcttgg
       61 tcccggtgtt ggcttccatg gcgctcgatc cgtgtgggtg actacctatc gaccggccgc
      121 ccggcacggc gcgcccgcgc cttgccctgg cggcctattt gccggcgatc gccagcagct
      181 tctctttgga cagaaccacc agccgcgtcg gctcgatgcg caccgcgcca tcgcgctcga
      241 agcctttcag ctcctggttg acgcgctggc gcgaggcgcc gagcaattgc gccaggtctt
      301 cctgcgcgag ctgcaggccg atgcggatct cttcgccctg cgcgatgccg tagctgcggg
      361 cgagcagcag gatctgcttg gccaggcgcg acgccagcgg ccgcgtgttc aggtcttcga
      421 ccgcatcgaa catcagccgc agccggcggc agttcaggcg cagcagcgct tcgtacagct
      481 cgctgtgctg cgccagcagc tccctgaagt cggccttgcg cacgaccagc aaggtggtct
      541 cgccatgggc gttggagtcg tgcgtgcgcg gcaggccgtc gaacagcgag atgtcgccga
      601 accaggtgcc cggctcgacg taggtgagcg tgatctgctt gcccgacagc gacaccgagc
      661 tgatgcgcac cgcgcccttc gcgacgccgc accactcctc ggccggtgcg ccgcgtgccg
      721 agagcatcgt gccgtcgctc aggcgccgca ccgccgcgcg tgacaggatc gcatggcgca
      781 gcgggggaga gagtctggaa aaccaagagc cggcgtcgat gttggagcgt tcggcgattg
      841 taagaacggg ggtattcatg atgagtcgct gatgtgacag catggggtcg ccgcgacggc
      901 ctattgtgtc cgacgcgggc ggcatcggcc gccgatcgca gacaacggag cccagcccat
      961 gaacccgccc accctcgaca tctcgagccg caagggccag gtcagtgccg aagaatggca
     1021 gacccgcgtc gacctcgcgg ccgcctaccg gctggtcgcg atgttcaagt gggacgacct
     1081 cgtcttcacc cacatcagcg cgcgcgtgcc gggccgcgac gacgcgttcc tgatcaaccc
     1141 ctacggcctg atgttcgacg agatcaccgc gtcgagcctg atcaaggtcg acgcgcaggg
     1201 ccacaagctc gatgcctcgg aatatgacgt gaacccggcc ggcttcacga ttcacagcgc
     1261 gatccacgcc gcgcgccacg atgcgcagtg cgtgctgcac gtgcacacgc tgaacggcgt
     1321 cgcggtctcg gcgcagaagg gcggggtgct gccgatctcg cagcagtcga tcttcgtgct
     1381 ctcgggcctg agctatcacg gctacgaagg cgtggcgctg cgcgacgacg agaagccgcg
     1441 gctggttgcc gacctcggcc ccgagaacaa cttcctgatg ctgcgcaacc atggcctgct
     1501 gaccctgggc gcgacgatcg ccgatgcgtt cctgaacatg tacctgttcg agaccgtctg
     1561 cacgatccag gtccgcgcga tggccggcgg caccgagctg atcccggtcg atgcgcgcat
     1621 catcgcgggc gcccaacagc aggcgaagca ggtcacgcgc ggcgtcggcg gcggcgcgct
     1681 ggcgtggccc ggcttgctgc gccggctcga cagggccgac ccgagctatc gcacttgacg
     1741 gcgccggccc gaccggggcg cgatcggccg atctgggcgg tgtttgcagg ccaatcatcc
     1801 gcgttggcaa ggccgcaacg atcgcggcgg gaactgcgtc acgccggcgc gttttgccgg
     1861 gctgcgcggg gccgtcgcgt gatgtcttca tgctagagtc gcccgcgaat ccaccagcga
     1921 cgaccgccaa ccatcgcccc caacgcatgc gcgcagtttc gaccctcacc gcgagctgcg
     1981 cgatcggcac tgcgctctgg ctggccgcct cgtcggccga cgcggtcggc ttcggtcgcg
     2041 tcaccaacac cacccagctc ggccagccgc tgaacttcgt cgcggccgtg cggctcgacc
     2101 ccgatgaact gctgccgcgc gagtgtgttt cggccgaagt gcagtcgggc gacaaccggc
     2161 tgcagccgca gcagatccgc gtcacgctcg aaggcgccgc cgatgcggcc gaccgcaacg
     2221 tgcgcgtcac gacgagcgca ctgatcgacg aaccggtcgt caccgtcagc gtcacgctcg
     2281 gctgcacatc aaaggtcacg cggcgcttcg tcgccttcat cgacccgccg ctgatcaacc
     2341 ccgcgcagac cgcgccggcc gaggccttgc cgccgcaacg catggactcg caggtcgcgc
     2401 cgatcgtcac gatggtgcag ggcgccgacg gctcagccgc ggtcgcgcgc cacgccgacc
     2461 gcggcggcac ggcgccacgc tcgcggccgg cggcgcgggt cggcgcgagc gcgggctcgg
     2521 gtgttgcgcg cgcgccggca gcgccgcgcg cagaggcccc gaaacgcgcc gcgccggcat
     2581 cgcgacagcc catcgtcgcg cgcgcggcgc cggcgggtgc gcggcttcag ctcgaggccg
     2641 ccgcgccggt cagcgcccgc agcgcctcgg ccgccgcctc cgcgccgatc gcggtgaaca
     2701 gcgccgcgcc ggcgccgatc aacgtcacgg ccgcgtccgc ggccg
//
LOCUS       DASRDG010000273         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_846104, whole genome shotgun sequence.
ACCESSION   DASRDG010000273 DASRDG010000000
VERSION     DASRDG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846104"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..2350)
                     /locus_tag="VFA35_04530"
     CDS             complement(44..2350)
                     /locus_tag="VFA35_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZT55469.1"
                     /translation="MRPKVALLLLAAAPAVPAQDRPVQDQADAPLPAPFAARCADCHG
                     ADAQKGGLDLATRARAGGVDWLLTLARARDRVAAGEMPPPGEAPLAADDKAQVLAFCA
                     ATLAQQVPRLPAQPGRVTVRRLSRTQWDNTVRDLFGVQSAKSAAFPADDLGYGFDTIG
                     DALSFSTLHLEQYLAAAKDVAAAVFDGEDPRAPAVRRVEAEHMTRVAGDGVSLDGDVA
                     GLWTNATIGTGVALPRDGTYRLRINAGADQAGNEPARMAVALDGKRLRVFDVPQRELR
                     VQELTAEMAGGVRRVELSFVNDFWDPKNPDPARRDRNLRIDWFELVGPLDARPLPPQR
                     RWLAQADRPDAPPPQRLRALAAALLPRAWRRPASGAEVERIAAVGEQALRRGEAFHLA
                     AQRALEAALVSPFFLFRIEGAANDGGGGGGGGDGATALPPAQLAVRLSCFLWSSAPDD
                     QLAAAAADGRLRDAAGRRAQVERMLADPRAESLATDFAAQWLELRALRDRTPDPARFP
                     CDAALKASMRRQTELLFLAVLRQRLDVRALLDCDFTFVDRRLAAFYGLDGSFGDEFVQ
                     VALPPAQRARGGLLGQASVLTVTSNPTRTSPVKRGKWILENLLGQAPPPPPPGNDSLK
                     DEPAASTPTGLRAQMALHRGKAACAGCHLRMDALGFALERFDAIGRPRERDEQGAIDD
                     SFALPDGRQLRGIDDLSGVLCADPAFPRTLAHKLFVFAVGRDLSAVDRLRLDLAVNAL
                     RARGAVTIADLVVAVVESDAFLLRGKTP"
     gene            2497..3480
                     /locus_tag="VFA35_04535"
     CDS             2497..3480
                     /locus_tag="VFA35_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZT55470.1"
                     /translation="MTRSSSLLFAVLLAFNLALVAWWTWFQVNETHELEAAGRLLANG
                     DVAGAARTFGAADVADLPARAAARRRMFVSEGVTFAIIALLGGLSVGAALRRDYSRRA
                     DQDRFLAGATHELKTPLATIQLLLESLQDDRLPPEKRARYMQSGLLEAQRLERGLTNV
                     LTAAGLRAHDRRLAARSDGDLAADVERAVAVMRPRAEAAGIELHAKLAPLAMTRDAEA
                     LQLVLHNLLDNAVKYSPRGSHVEIALRDEDGHAVVSVRDQGQGMDAEALRNAFVPFWR
                     GRDSAAGGTGLGLHLVRELVRAHGGTVAAASGGPGRGSEFTVRLPRRGGNA"
     gene            3477..4163
                     /locus_tag="VFA35_04540"
     CDS             3477..4163
                     /locus_tag="VFA35_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZT55471.1"
                     /translation="MSRWILVAEDEGPLGEMLCDNLALESYHAELVQTGPDALLRMQR
                     GGIDLLVLDIMLPGCDGFEVLRQLRARGDDTPVLVLSARNSDRDRIKGLELRADDYLT
                     KPFNLRELLLRVDALLRRRPAPSPGADTLEFGGNAVDFRAMRARDFRGEEHELTATEV
                     KLLKLLAAHAGTVVSRKIVVEHLFGSTAPLTVRTLDNVVLRLRKLFEPEPAAPKHLHT
                     VRGLGLRFQP"
     gene            complement(4180..>4517)
                     /locus_tag="VFA35_04545"
     CDS             complement(4180..>4517)
                     /locus_tag="VFA35_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZT55472.1"
                     /translation="GLRGSIANGRLGKEAITMVEPVEVLRGATLVACRLQTGRTHQIR
                     IHLAEHGHMLLGERGYVREHRGPALAAPRILLHAAELGFVHPDNGEPLRFSSPLPDDM
                     AAVLARLRP"
BASE COUNT          566 a         1687 c         1725 g          539 t
ORIGIN      
        1 cgcaggaagg tgcggcggtc gagcgcgcgg ttgcggaacg gcgtcacggc gtcttccccc
       61 gcagcaggaa cgcgtcgctc tcgaccaccg cgaccacgag gtccgcgatc gtcaccgcgc
      121 cgcgcgcgcg cagcgcgttg acggcgaggt cgagacgcag gcggtcgacg gcagagaggt
      181 cgcggccgac cgcgaacacg aacagcttgt gcgcgagcgt gcgcgggaac gcgggatcgg
      241 cgcacagcac gccgctcaga tcgtcgatgc cgcgcagctg ccggccgtcg ggcagcgcga
      301 acgaatcgtc gatcgcgccc tgttcgtcgc gttcgcgcgg ccggccgatc gcgtcgaagc
      361 gttcgagcgc gaagccgagc gcgtccatgc gcaggtgaca gcccgcgcac gcggccttgc
      421 cgcggtgcag cgccatctgc gcgcgcagac cggtcggcgt cgacgcggcg ggctcgtcct
      481 tcagcgagtc gttgccgggc ggcggcggcg gcggcgcctg gccgagcagg ttctcgagga
      541 tccacttgcc gcgcttgacg ggcgacgtgc gcgtcggatt ggacgtcacg gtgagcacgc
      601 tcgcctggcc gagcagcccg ccgcgcgcgc gctgcgcggg cggcagcgcc acctgcacga
      661 actcgtcgcc gaagctgccg tcgaggccgt agaacgccgc gagccgccgg tcgacgaacg
      721 tgaagtcgca gtcgagcagc gcgcgcacgt cgaggcgctg ccgcagcacg gcgaggaaca
      781 gcagctccgt ctggcgccgc atcgacgcct tcagcgccgc gtcgcacggg aagcgcgcgg
      841 gatccggcgt gcggtcgcgc agcgcgcgca gctcgagcca ctgcgccgcg aagtcggtcg
      901 ccagcgactc ggcgcgcgga tccgcgagca tgcgctcgac ctgcgcgcgg cgccccgcgg
      961 cgtcgcgcag ccggccgtcc gcggccgccg cggcgagttg gtcgtcgggc gcgctgctcc
     1021 agaggaagca gctcaggcgc accgcgagct gcgcgggcgg cagcgccgtg gccccgtcgc
     1081 cgccgccacc gccgccgccg ccgtcgttcg cggcgccttc gatccggaac aggaagaacg
     1141 gcgacaccag ggccgcctcc agcgcgcgct gcgctgcgag gtggaacgct tcgccgcgcc
     1201 gcagcgcctg ctcgccgacg gcggcgatgc gttcgacctc ggcgccgctc gcgggccttc
     1261 gccatgcgcg cggcagcagc gccgccgcga gcgcgcgcag ccgctgcggc ggcggcgcat
     1321 cggggcggtc ggcctgcgcg agccagcgcc gctgcggcgg cagcggccgc gcgtcgagcg
     1381 gaccgaccag ctcgaaccag tcgatgcgca ggttgcggtc gcggcgcgcg gggtcggggt
     1441 tcttcgggtc ccagaagtcg ttgacgaacg acagctcgac gcggcgcacg ccgccggcca
     1501 tctccgcggt gagctcctgt acgcgcagct cgcgctgcgg cacgtcgaac acgcggagcc
     1561 gcttcccgtc gagtgcgacg gccatccgcg ccggctcgtt gcccgcctga tcggcgccgg
     1621 cattgatgcg cagccggtag gtgccgtcgc gcggcagcgc gaccccggtg ccgatcgtcg
     1681 cgttggtcca cagccccgcg acgtcgccgt cgagcgacac gccgtcgccc gcgacgcgcg
     1741 tcatgtgctc ggcctcgacg cggcgcaccg ccggcgcgcg cgggtcctcg ccgtcgaaca
     1801 cggcggcggc gacgtccttg gccgcggcga ggtactgctc gaggtgcagc gtggagaacg
     1861 acagcgcgtc gccgatggtg tcgaagccgt agccgaggtc gtcggcgggg aacgcggccg
     1921 acttcgcgct ctgcacgccg aacaggtcgc gcacggtgtt gtcccactgc gtgcgcgaca
     1981 gccggcgcac ggtcacgcgg cccggctgcg ccggcagccg cggcacctgc tgcgcgagcg
     2041 tcgcggcgca gaacgcgagc acctgcgcct tgtcgtccgc ggcgagcggg gcctcgcccg
     2101 gcggcggcat ctcgccggcg gcgacgcggt cgcgggcgcg cgcgagcgtc agcagccagt
     2161 cgacgccccc cgcgcgtgcg cgcgtggcga ggtcgaggcc ccccttctgc gcgtcggcgc
     2221 cgtggcagtc ggcgcagcgg gcggcgaacg gtgccggcaa cggcgcgtcg gcctgatcct
     2281 ggactggccg atcctgggcc gggacggccg gcgccgcggc gagcaacagc aacgcgacct
     2341 ttgggcgcac gtcgcggcca ggatagcccg ccccgagcgc cgtggcacga gcgccgtggc
     2401 acgggcgccg tggcacgggc ggttcgcggc cgccggcgcc gaggagcgcg gcccgcgccg
     2461 ttcggctacg ctgtggcgga ggactgatcg ccgcgcgtga cccgttcgag ttcgctgctg
     2521 ttcgccgtgc tgctcgcgtt caacctcgcg ctggtcgcct ggtggacgtg gttccaggtc
     2581 aacgagaccc acgagctcga ggccgcgggc cggctgctcg cgaacggcga cgtcgcgggc
     2641 gccgcgcgca cgttcggcgc ggcggacgtc gccgacctgc cggcgcgcgc ggcggcgcgg
     2701 cggcgcatgt tcgtcagcga aggcgtcacg ttcgcgatca tcgcgctgct cggcggcctg
     2761 tcggtcggcg ccgcgctgcg ccgcgactac agccgccgcg ccgaccagga ccgcttcctc
     2821 gccggggcca cgcacgagct caagacgccg ctggcgacga tccagctgct gctcgaatcg
     2881 ctgcaggacg accgcctgcc tcccgagaag cgcgcgcgct acatgcagtc gggactgctc
     2941 gaggcgcagc ggctcgagcg cggcctcacc aacgtgctca ccgccgccgg gctgcgcgcg
     3001 cacgaccgcc ggctcgcggc gcgcagcgac ggcgacctcg ccgccgacgt cgaacgcgca
     3061 gtcgcggtga tgcggccgcg cgcggaggcg gcgggcatcg agctgcacgc gaagctcgcg
     3121 ccgctggcga tgacgcgcga cgccgaggcg ctgcagctcg tgctgcacaa cctgctcgac
     3181 aacgccgtca agtactcgcc gcgcggcagc cacgtcgaga tcgcgctgcg cgacgaggac
     3241 ggccacgcgg tggtgtcggt gcgcgaccag ggccagggca tggacgccga ggcgctgcgc
     3301 aacgcgttcg tgccgttctg gcgcgggcgc gactcggccg ccggcggcac ggggctcggc
     3361 ctccacctgg tgcgggagct ggtgcgcgcg cacggcggca cggtcgccgc ggcctcgggc
     3421 ggcccgggcc gcggcagcga attcacggtg cggctgccgc gccgcggagg caacgcatga
     3481 gccgctggat cctggtcgcc gaggacgagg gaccgctcgg cgagatgctg tgcgacaacc
     3541 tcgcgctcga gagctaccac gcggagctcg tgcagaccgg ccccgacgcg ctccttcgca
     3601 tgcagcgcgg cggcatcgac ctgctggtgc tcgacatcat gctgccgggc tgcgacggct
     3661 tcgaggtgct gcggcagctg cgcgcgcgcg gcgacgacac gccggtgctc gtgctgagcg
     3721 cgcgcaactc cgaccgcgac cgcatcaagg gcctcgagct gcgcgccgac gactacctga
     3781 cgaagccgtt caacctgcgc gagctgctgc tgcgcgtcga cgcgctgctg cggcggcggc
     3841 cggcgccgtc gcccggtgcc gacacgctcg agttcggcgg caacgcggtc gacttccgcg
     3901 ccatgcgcgc gcgcgacttc cgcggcgagg agcacgaact caccgcgacc gaggtcaagc
     3961 tgctcaagct gctcgccgcg cacgcgggca cggtggtgag ccgcaagatc gtcgtcgagc
     4021 acctgttcgg cagcacggcg ccgctgaccg tgcgcacgct cgacaacgtc gtgctgcggc
     4081 tgcgcaagct gttcgagccg gaacccgcgg cgccgaagca cctgcacacc gtgcgcgggc
     4141 tcggtctgcg cttccagccg tgatgcgcag acggcgcgct cacggacgca gccgcgcgag
     4201 caccgccgcc atgtcgtcgg ggagcggcga gctgaagcgc agcggctcgc cgttgtcggg
     4261 atgcacgaag ccgagctcgg cggcgtgcag caggatgcgc ggcgcggcca gcgccgggcc
     4321 gcggtgctcg cgcacgtagc cgcgctcgcc gagcagcatg tggccgtgct cggcgaggtg
     4381 gatgcggatc tggtgcgtgc gccccgtctg cagccggcag gcgaccagcg tcgcgccgcg
     4441 cagcacctcg accggctcga ccatcgtgat cgcctccttg ccgagcctgc cgttggcgat
     4501 cgacccacgc agcccgt
//
LOCUS       DASRDG010000274         4677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_612909, whole genome shotgun sequence.
ACCESSION   DASRDG010000274 DASRDG010000000
VERSION     DASRDG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612909"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VFA35_04550"
     CDS             <1..905
                     /locus_tag="VFA35_04550"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZT55473.1"
                     /translation="RLLYGAGAYGHPAGGTPSSLARVTRAELVALHAARYRPDNAALV
                     LAGDLDAASARRLADRHFGAWQAGGGTAPSSAAPAGAALPQAAAVIDMPQSGQAAVVA
                     AAKLPPLGADRATAAVLNAVLGGGFSSRLNQEIRIRRGLSYSAGSALDARPGGGSLRA
                     VVQTKNESAAEVVTLVQAELDRLAAAPVGADELAARKATLIGNFSRSVETTAGLGAAV
                     KSLIVAGLPPDDLRTRIEALSAVTAADVQRYAASNLGAAGRRVVVAGESAKFVEALRT
                     ELPGVVVVPAARLDLDRDSGLSAP"
     gene            1070..1327
                     /locus_tag="VFA35_04555"
     CDS             1070..1327
                     /locus_tag="VFA35_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55474.1"
                     /translation="MATRRPPSPTASDTPQKLRQTQAEYVAARPAGEPGVKPMMSSSK
                     MYVCRRCQANFKTGDGKPDARLPGLGKCNKCLAAATTALAA"
     gene            complement(1344..3056)
                     /gene="ybaL"
                     /locus_tag="VFA35_04560"
     CDS             complement(1344..3056)
                     /gene="ybaL"
                     /locus_tag="VFA35_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HZT55475.1"
                     /translation="MRHDVSLISTIAAGFGLAMVFGYIAARIKVPPLVGYLFAGILIG
                     PATPGFVADVDLAGQLAEIGVMLLMLGVGLHFSLKDLLAVRRIAIPGALLQIAVATAL
                     GMGTAALWGWGLGAALVFGLALSVASTVVLLRALESRGVLESVNGRIAVGWLIVEDLV
                     MVLVLVLLPALAPLLGGGAQGHPAPDSNIWATLGITLAKVGAFIALMLVVGRRVFPKL
                     LWLVARTGSRELFTLCVIAGAVSVAYGSAKLFDVSFALGAFFAGMMMRESEFSHRAAE
                     ESLPLRDAFSVLFFVSVGMLFDPRVLLEEPLRVLAVVAIVMLGKTLAAVVLVVAFRYP
                     LNSALTVGASLAQIGEFSFILAGLGVALKLLPVEGQSLILAGALISIALNSLLFGAIE
                     PVQAWLRERSALARRLERPDDPLAELPTSTDETLLTGQVVLVGHGRVGKRIAQVLTAR
                     RVPFVVAEQNREFVEALRAQGVPAVSGDASEPSVLIQAHIARAAMLVIATPDTLGVRK
                     MVAVARALNPGIEVVVRSHNEEEAKLLEAEHVGKVFIGEHELAHGMAGHVLARMVRGL
                     AADH"
     gene            complement(3152..3403)
                     /locus_tag="VFA35_04565"
     CDS             complement(3152..3403)
                     /locus_tag="VFA35_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55476.1"
                     /translation="MHFDERKIGEYRVFTGALEGPMGDGYIAALIVQREQGGGVPREI
                     VRDESLACGHRWPSPDAALAYAMGKAQDLIRKQAPALAC"
     gene            complement(3521..4051)
                     /locus_tag="VFA35_04570"
     CDS             complement(3521..4051)
                     /locus_tag="VFA35_04570"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HZT55477.1"
                     /translation="MSDPGPIQMSGDMHSFDAHYDAEGSARAERAFHVRAIRELRPWS
                     TFGLPALLSVLVAAGSAIGAPGWFLLSFGLLLLASVLVPAFFYFARPRAAARLARQHP
                     VRRISLSAQALTMCVGERSVVVEWREVIRIWEAPGYLLLVVGKFTAVSIPVSSLPTGA
                     REFIESSAKSAKAVGG"
     gene            complement(4083..4457)
                     /locus_tag="VFA35_04575"
     CDS             complement(4083..4457)
                     /locus_tag="VFA35_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55478.1"
                     /translation="MIVAVFDSVDVAEKAGRDFRGIEDKDEGFKIERGVLVQKDATGR
                     LIVLDQPTRPFWASVLGAITGGLADHAIKDLLDGEWVASISGQLAPGAVVFVLQAQEA
                     SPFAIDNIVLGYRGTVFRKAFV"
BASE COUNT          799 a         1704 c         1558 g          616 t
ORIGIN      
        1 aacggctgct gtacggcgcg ggcgcctacg gccacccggc cggcggcacg ccgagctcgc
       61 tggcgcgggt cacgcgggcc gagctggtgg cgctgcacgc ggcacgctat cggcccgaca
      121 acgcggcgct ggtgctggcc ggcgatctcg atgccgcatc ggcgcgccgg ctcgccgacc
      181 gccacttcgg cgcgtggcag gcgggcggcg gcaccgcgcc ttcgagcgcg gcgcccgccg
      241 gcgcggcgct gccgcaggcc gccgcggtga tcgacatgcc gcagtccggc caggccgcgg
      301 tcgtcgccgc agccaagctg ccaccgctgg gcgccgaccg cgcgaccgcc gcggtgctga
      361 acgcggtgct cggcggcggc ttctcgtcgc ggctgaacca ggagatccgc atccggcgcg
      421 gcctgagcta ttcggccggc agtgcgctcg acgcccgccc gggcggcggt tcgctgcgcg
      481 cggtggtcca gaccaagaac gaatcggcgg ccgaggtcgt gacgctggtg caggccgaac
      541 tcgaccggct ggccgccgca ccggtcggcg ccgacgagct cgcggcgcgc aaggccacgc
      601 tgatcggcaa cttcagccgc agcgtcgaga cgacggccgg gctcggcgcg gcggtcaagt
      661 cgctgatcgt cgccgggctg ccgccggacg atctgcgcac ccgcatcgag gcgctgtcgg
      721 cggtcaccgc cgccgatgtg cagcgctatg ccgcgtcgaa cctgggcgcg gcgggccggc
      781 gcgtggtcgt cgcgggggaa tcggccaagt tcgtcgaggc gctcaggacc gaattgccgg
      841 gcgtcgtggt cgtgccggca gcccggctcg atctcgaccg cgacagtggc ctgagcgccc
      901 cgtgagcgcg gccggagcgt gcagtgcgcg tgaccaactg aaacacaatc cctcgaatcg
      961 cagcgaccct cgatcgcgcg tggcatcggg attgctggga caatcgagga ttgcccgtga
     1021 ttggcgtcaa gccccacggt cgtccgcact tctcttttca ggaccgccca tggccacccg
     1081 tcgtcccccc tcccccaccg cctcggacac cccccaaaaa ctgcgtcaaa cccaggccga
     1141 atacgtcgcc gcccggccgg ccggcgaacc cggcgtcaag ccgatgatgt cgagctcgaa
     1201 gatgtacgtc tgccgccgct gccaggccaa cttcaagacc ggcgacggca agcccgatgc
     1261 acgcctgccg ggcctcggca agtgcaacaa gtgcctcgcg gccgcgacca ccgcactcgc
     1321 cgcctgatcg gcggcagcac cctctaatgg tccgcggcaa gcccgcggac catacgcgcc
     1381 agcacatgcc cggccatgcc gtgcgccagt tcgtgctcgc cgatgaagac cttgccgaca
     1441 tgctcggcct ccagcagctt cgcctcctcc tcgttgtggc tgcgcacgac cacctcgatg
     1501 cccggattca gcgcgcgcgc caccgccacc atcttgcgca cgccgagcgt gtcgggcgtc
     1561 gcgatcacca gcatcgcagc gcgcgcgatg tgcgcctgga tcagcaccga gggctcgctc
     1621 gcatcgccgc tgaccgccgg cacgccctgc gcgcgcagcg cctcgacgaa ctcgcggttc
     1681 tgttcggcga cgacgaacgg gacgcgtcgc gccgtgagca cctgcgcgat gcgcttgccg
     1741 acgcggccat gaccgaccag cacgacctgc ccggtcaaca gcgtctcgtc ggtgctcgtc
     1801 ggcaactcgg cgagcgggtc gtcgggccgc tccagccgcc gcgccagcgc cgagcgctcg
     1861 cgcagccagg cctgcaccgg ttcgatcgcg ccgaacagca gtgaattcaa cgcgatcgag
     1921 atcaatgcgc cggccaggat caggctctgc ccttcgaccg gcagcagttt cagcgcgaca
     1981 cccaggcccg cgagaatgaa cgagaactcg ccgatctgtg cgaggctcgc gccgacggtc
     2041 agcgcgctgt tcagcgggta gcggaacgcg accaccagca cgaccgcggc gagcgtcttg
     2101 ccgagcatga cgatcgcgac caccgcgagc acgcgcagcg gctcctcgag cagcacgcgc
     2161 gggtcgaaca gcatgccgac cgagacgaag aacagcaccg agaacgcgtc gcgcagcggc
     2221 agcgactcct cggcggcgcg gtggctgaac tccgactcgc gcatcatcat cccggcgaag
     2281 aacgcgccga gcgcaaacga cacatcgaac agcttggccg acccatacgc gacgctgacc
     2341 gcgccggcaa tcacgcacag cgtgaacagc tcgcgcgagc cggtgcgcgc gaccagccac
     2401 agcagcttcg ggaacacgcg ccggccgacg accagcatca gcgcgatgaa cgcgccgacc
     2461 ttcgcaagcg tgatgccgag cgtggcccag atgttcgagt cgggcgcggg gtgaccttgc
     2521 gcgccgccac cgagcagcgg cgcgagggcc ggcagcagca ccagcacgag caccatcacc
     2581 aggtcttcga cgatcagcca gccgaccgcg atccggccgt tgaccgattc gagcacgccg
     2641 cgcgattcga gcgcacgcag cagcacgacc gtgctcgcga ccgagagcgc gaggccgaac
     2701 acgagcgcag ccccgaggcc ccagccccac agcgcggccg tgcccatgcc gagcgcggtc
     2761 gcgaccgcaa tctgcagcaa cgcaccgggg atcgcgatgc gcctgaccgc gagcagatcc
     2821 ttcagcgaga agtgcagccc gacgcccagc atcagcagca tcacgccgat ctcggccaac
     2881 tgcccggcga gatcgacatc ggcgacgaag cccggcgtgg ccggcccgat caggatgccg
     2941 gcaaacaggt agccgaccag cggcggcacc ttgatgcgcg ccgcgatgta gccgaagacc
     3001 atcgccaagc cgaatcccgc ggcaatcgtg gagatcaggc tgacgtcatg gcgcatcggg
     3061 ggagactttc tgcgcacaag ctcgggttcg cgcagagtct aaaggcagcc ctcgtggggg
     3121 tgccgggcga aggcccggcc cttgcgggcg ctcagcaggc gagcgccggc gcctgcttgc
     3181 ggatcaggtc ttgcgccttg cccatcgcgt aggccagcgc cgcatcgggc gagggccagc
     3241 ggtgaccgca ggccaggctc tcgtcgcgca cgatctcgcg cggcacgccg ccgccctgct
     3301 cgcgctgcac gatcaacgca gcaatgtagc catcgcccat cgggccttcc agcgcgccgg
     3361 tgaagacgcg gtactcgcca atcttgcgtt cgtcgaagtg catgtgatcc tctccggccg
     3421 gcatcccatc gcccggcaag gcacggatca tctgcgcttt cgccgcggcg cggtatccac
     3481 cgaatggggg aaaatgtcac gcagcgccgt cacggcgtcg tcacccgcca acggcctttg
     3541 cgctctttgc cgaggattcg atgaactctc gcgctcccgt gggcaggctg gacaccggga
     3601 tgctgacggc ggtgaacttg cccacaacca gcaacaggta gccgggcgcc tcccagatgc
     3661 ggatgacctc gcgccactcg acgaccacgc tgcgctcgcc gacgcacatc gtcagcgcct
     3721 gcgccgacag gctgatgcgc cgcaccgggt gctggcgggc cagcctggcc gccgcgcgcg
     3781 gccgcgcgaa gtagaagaag gcaggcacca gcacggacgc cagcagcaac aggccgaacg
     3841 acaacaggaa ccagcccggc gcgccgatcg ccgagcccgc cgccaccagc accgacagga
     3901 gcgccggcag gccgaacgtg gaccaaggcc gcagttcgcg gatcgcgcgg acgtggaatg
     3961 cgcgctcggc gcgcgccgag ccttctgcgt catagtgagc gtcgaaggaa tgcatgtcac
     4021 ccgacatttg aatggggccg ggatcgctca cggcaggttg cccggcacct tggccggggg
     4081 aatcacacga atgctttgcg aaacacggtc ccgcgatagc cgagcacgat gttgtcgatg
     4141 gcgaacggcg atgcctcttg cgcctggaga acgaagacaa ccgcgccagg ggcgagctgg
     4201 ccggatatcg atgcgaccca ttcgccgtcg agcaggtctt tgatcgcatg gtcggccagg
     4261 ccgcccgtga tcgcacccag cacgctggcc cagaagggtc gcgtcggctg gtcgagcacg
     4321 atcaatcggc ccgtggcgtc cttctgaacc agcacgccgc gctcgatctt gaagccttcg
     4381 tccttgtcct cgatgccgcg gaagtcgcga ccggcctttt ccgcgacatc cacgctgtcg
     4441 aacaccgcga cgatcaactg ttgtgccatg ggatggttcc tcgcatcgat gacgcacccg
     4501 acgcctgaaa tcattgtagt cagcgtctct ggtcggcccc gtcgaaccga gcgctcgagg
     4561 tggcatgatt aatgaaagcc ggacgctgta tccggatgcc tcgaagagtc accatgccga
     4621 aacagctcta cagccaactc gtcgaggacg tcgtcaagag cgtggacgac ggggtcc
//
LOCUS       DASRDG010000275         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_846755, whole genome shotgun sequence.
ACCESSION   DASRDG010000275 DASRDG010000000
VERSION     DASRDG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846755"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..>2311)
                     /locus_tag="VFA35_04580"
     CDS             complement(25..>2311)
                     /locus_tag="VFA35_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZT55479.1"
                     /translation="SRVNPDTATRPGSRCAEADFQSLAETLSSLIVLIRGDKILYVNP
                     AACALLGRPREWFLGRDFWDAVYPDDREAAIARGRARTAGIAQPKRLTERLLHADGSA
                     VWMDFSIDVVLFEGQPTTLCTGHDVTERLRVEEELRRSEARLAEAQRIGNVGSWEWDV
                     ASGRVTWSDEMCRIFAFCPRQFGASVAGYLELVHPEDRPIAQLVMEQALERGGSFAFE
                     HRIVRQDGVVRTIFGRGEVFRGADGKPVRVAGTGQDISEKKRIAQELQQSEERFGNLC
                     TQAPVMLMSFDHDKRIRDVSNFWLQTTGYERHEVIGRNGFDFITPDSRERLLQAIEQN
                     RSNNEVVLRNLPLRGVCKDGSTIDLLSTSVAEIGDGGEHKGAICVQINLTDLRRAEAA
                     LHESAERYRALVEHAPEAIMVLDVDLGRFVDANAHAERLFGWSRERLLTMGPLDFCPE
                     QQADRRASCEVIEDEHRKVLAGGTAEFEFAHVDAAGREIIYQTRLSRLPAAGRQLIRA
                     TITDVTELKHLQEKVRHAEKLAAVGVLAAGVAHEIGNPLMALSMAAQSLERRTQDAYA
                     QGKLKLIREHIDRISRIVWQMSDLARPPSGRRGVHDLNHIVRRAVEMVRYDRRARNAE
                     VHYQLAEQLPGVEIVEDELTQVCINLALNAFDAMAANPPGRPRRLHIRSASADGLLRV
                     TFADTGPGVPRELRTKLFQPFFTTKEVGLGSGLGLSVSYRILQEHRGTLALDENVTDG
                     AAFAFELPVGLPIGLPKGGGR"
BASE COUNT          308 a          810 c          834 g          359 t
ORIGIN      
        1 cgtcgacgac caggatgcgg aacgtcatcg accgccaccc ttgggcaagc cgatgggcag
       61 tccgacgggc agctcgaagg cgaacgccgc gccgtcggtg acgttctcgt cgagcgccag
      121 cgtgccgcgg tgctcctgga ggatgcggta gctgaccgac agcccgaggc cgctgccgag
      181 gcccacctcc ttggtggtga agaacggctg gaacagcttg gtgcgcagct cgcgcggcac
      241 gcccggcccc gtgtcagcga acgtgacgcg cagcaggccg tcggcgctcg ccgagcggat
      301 gtgcaggcgg cggggccgcc cgggcgggtt ggcggccatc gcgtcgaacg cgttcagcgc
      361 caggttgatg cagacctgcg tcagctcgtc ctcgacgatc tcgacgccgg gcagctgctc
      421 ggcgagctgg tagtggacct cggcgttgcg cgcccggcgg tcgtagcgga ccatctcgac
      481 cgcgcgccgc acgatgtggt tgaggtcgtg cacgccgcgc cgcccgctcg gcgggcgcgc
      541 caggtcgctc atctgccaca cgatgcgcga gatgcggtcg atgtgttcgc ggatcagctt
      601 cagcttgccc tgcgcgtagg cgtcctgcgt gcgccgctcg aggctctgcg ccgccatcga
      661 cagggccatc agcgggttgc cgatctcgtg cgcgacgccg gcggcgagca cgccgacggc
      721 agccagcttc tcggcgtggc gcaccttctc ctgcaggtgc ttgagctccg tgacgtcggt
      781 gatcgtggcg cggatcagct ggcggccggc ggccggcagg cgcgacagcc gcgtctggta
      841 gatgatctcg cggccggcgg cgtcgacgtg cgcgaactcg aactcggcgg tgccgcccgc
      901 gagcaccttg cggtgctcgt cctcgatgac ctcgcaggac gcgcgccggt cggcctgctg
      961 ctccggacag aagtcgagcg gccccatcgt cagcagccgc tcgcgcgacc agccgaacaa
     1021 acgctccgcg tgcgcattgg cgtcgacgaa gcggccgagg tcgacgtcga gcaccatgat
     1081 cgcctcgggc gcgtgctcga cgagcgcgcg gtagcgctcg gcgctctcgt gcagcgccgc
     1141 ctctgcgcgg cgcaggtcgg tgaggttgat ctgcacgcag atcgcgccct tgtgctcgcc
     1201 gccgtcgccg atctcggcca cgctcgtgct gagcaggtcg atcgtgctgc cgtccttgca
     1261 gacgccgcgc aggggcaggt tccggagcac gacctcgttg ttgctccggt tctgctcgat
     1321 cgcctgcagc agccgttcgc gcgaatcggg cgtgatgaag tcgaagccgt tgcggccgat
     1381 cacctcgtgg cgctcgtagc cggtcgtctg cagccagaag ttgctgacgt cgcggatgcg
     1441 cttgtcgtgg tcgaacgaca tcagcatcac cggcgcctgc gtgcagaggt tgccgaaccg
     1501 ctcctcgctc tgctggagct cctgcgcgat gcgcttcttc tcgctgatgt cctggccggt
     1561 gcccgcgacc cgcaccggtt tgccgtccgc gccgcggaac acctcgccgc ggccgaagat
     1621 cgtgcgcacg acgccgtcct ggcgcacgat ccggtgctcg aacgcgaacg aaccgccgcg
     1681 ctccagcgcc tgctccatga ccagctgcgc gatcggccgg tcctcggggt gcaccagctc
     1741 gaggtagccg gcgaccgacg cgccgaactg ccgcgggcag aaggcgaaga tgcggcacat
     1801 ctcgtcggac caggtgacgc ggccgctggc cacgtcccac tcccagctgc cgacgttgcc
     1861 gatgcgctgc gcctcggcga gccgggcctc gctgcgccgc agctcctcct cgacgcgcag
     1921 gcgctcggtg acgtcgtggc cggtgcacag cgtcgtcggc tgcccttcga acaacacgac
     1981 gtcgatcgag aagtccatcc agaccgcgct gccgtcggcg tgcagcagcc gttcggtgag
     2041 tcgcttgggc tgcgcgatgc cggccgtgcg cgcgcggccg cgcgcgatcg ccgcctcgcg
     2101 gtcgtcggga tacaccgcgt cccagaagtc gcgcccgagg aaccactcgc gcgggcggcc
     2161 gagcagggcg caggcggcgg ggttgacgta gaggatcttg tcgccgcgga tcaggacgat
     2221 cagcgaggag agggtctcgg cgagggactg gaagtccgct tcggcgcacc gggacccggg
     2281 acgggtggcg gtatctgggt tcacgcggct t
//
LOCUS       DASRDG010000276         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1194466, whole genome shotgun sequence.
ACCESSION   DASRDG010000276 DASRDG010000000
VERSION     DASRDG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194466"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VFA35_04585"
     CDS             <1..581
                     /locus_tag="VFA35_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZT55480.1"
                     /translation="GIDFEAEVAVVTGDIPMGTDPEAAIDGIRLVMLANDVSLRNLIP
                     AELAKGFGFVQSKPATAFSPVAVTPDELLHAWRGGRVHLNLESTWNGKRVGLCDAGDE
                     MTFHFGQLIAHICKTRNVRAGSIVGSGTVSNKDWSRGYSCIAEKRAIETIEGGTPKTA
                     FMQFGDTIRIEMKGLDGMSVFGAIEQRVARTA"
     gene            595..1086
                     /locus_tag="VFA35_04590"
     CDS             595..1086
                     /locus_tag="VFA35_04590"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HZT55481.1"
                     /translation="MNNDLPRGLKLITIWLLVGAAVFVAIQWWQHRAQQTRFQVSGGT
                     LEIRRGDDGHYHWPGRVNGRRVDFLIDTGATGTAIPASLAHDLKLESLGTVRSNTAGG
                     IATGQVVRTDIELQGGVRVERLEIVALPGLRDSPLLGMDVLGKLHWQQRDGVLRVELA
                     PPR"
     gene            1201..1869
                     /locus_tag="VFA35_04595"
     CDS             1201..1869
                     /locus_tag="VFA35_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HZT55482.1"
                     /translation="MTDPSPFSKLVPGFDFLQGLVKNAGAALPGIGQWVAPTIDPAEL
                     EKRIEELRTVQFWLEQNARMLGATIQALEVQRMTLSTLKTMNVQMEGLRDSLQIRMPT
                     VSRPAPAPEPEPEPEPAPAPAEPAASDAAPAADAAVPAPGVVDPLQWWGALTKQFTEL
                     ATTAMKDTASDAARKLASAVVKTSLDAAPRAAKAPKAAAPAAKKRKPAAKKKAPAKKA
                     ASRA"
     gene            1888..>2517
                     /locus_tag="VFA35_04600"
     CDS             1888..>2517
                     /locus_tag="VFA35_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HZT55483.1"
                     /translation="MNRFLHGHATHPDWRVALALAAAQIDAQREQPGTAAAPTLGWVY
                     LTDALAAHAAALLAELGRRWPGVEWVGASGVGIAAGGVEYFDEPALSLLLTDLAPAHF
                     RVFSGARPLAGFAAHTANVHADPGTADLADLIHDMSARTATGYLFGGLASGRSHPLHV
                     AGGVLSGGLSGVAFDRSVPLVSRVTQGCQPVGPQRRVSAAERNVVVTLDG"
BASE COUNT          397 a          914 c          887 g          319 t
ORIGIN      
        1 ggggcatcga cttcgaggcc gaggtcgcgg tggtcaccgg cgacatcccc atgggcacgg
       61 accccgaggc ggcaatcgac ggcatccgcc tggtgatgct ggccaacgac gtgagcctgc
      121 gcaacctgat cccggccgaa ctcgcgaagg gcttcggctt tgtgcagagc aagcccgcga
      181 ccgcgttcag cccggtcgcg gtgacgcccg acgagctctt gcatgcgtgg cgcggcgggc
      241 gcgtgcacct gaacctcgaa agcacctgga acggcaagcg cgtcggcctg tgcgacgccg
      301 gtgacgagat gaccttccac ttcggccagc tgatcgcgca catctgcaag acccgcaacg
      361 tgcgcgccgg cagcatcgtc ggcagcggca cggtcagcaa caaggactgg tcgcgcggct
      421 actcgtgcat cgccgagaag cgcgcgatcg agacaatcga gggcggcacg ccgaagacgg
      481 cgttcatgca gttcggcgac acgatccgga tcgagatgaa ggggttggac gggatgagcg
      541 tgttcggggc gatcgagcag agggtggcac gcaccgcctg atgacgcgcc cagcatgaac
      601 aacgacctcc cccgcggcct caagctgatc acgatctggc tgctcgtcgg tgccgccgtg
      661 ttcgtcgcga tccagtggtg gcagcaccgg gcgcagcaga cacgcttcca ggtcagtggc
      721 ggcacgctcg agattcgccg tggcgacgac ggccactacc actggccggg gcgcgtcaac
      781 ggccggcgcg tcgacttcct gatcgacacc ggcgcgaccg gcaccgcgat cccggcgtcg
      841 ctggcgcacg acctgaagct cgaatcgctc ggcacggtgc gctcgaacac ggcgggcggc
      901 atcgccaccg gccaggtcgt gcgcaccgac atcgagctgc agggcggcgt gagggtcgag
      961 cggctcgaga tcgtcgcgct gcccgggctg cgcgacagcc cgctgctcgg catggacgtg
     1021 ctcggcaagc tgcactggca gcagcgcgac ggcgtgctgc gcgtcgaact cgcgccgccg
     1081 cgctgagcgc cgcgatggtt cgcatgctcg gcgcgaccgg tttaccgcca cggatggccg
     1141 agcgcgggta tcgtgtcgaa cgcatcgtcc acaaccaaca aaccacagga gacactcacc
     1201 atgaccgatc cgtccccatt ctcgaagctc gtgccggggt tcgacttttt gcagggcctg
     1261 gtgaagaacg ccggggccgc gctgccgggc atcggccagt gggttgcgcc gacgatcgac
     1321 cccgccgagc tcgaaaagcg catcgaggaa ctgcgcaccg tgcagttctg gctcgagcag
     1381 aacgcgcgca tgctcggcgc gacgatccag gcgctcgagg tgcagcgcat gacgttgtcg
     1441 acgctgaaga cgatgaacgt gcaaatggaa gggctgcgcg attcgcttca gatccgcatg
     1501 ccgaccgtct cgcggcccgc accggccccc gaaccggagc ccgaacccga accggccccg
     1561 gcgcccgcgg aacccgcggc gtcggacgcc gcgcctgccg ccgacgcggc agtgcccgcg
     1621 cccggcgtcg tcgacccgtt gcagtggtgg ggcgcgctga ccaagcagtt caccgaactc
     1681 gcgaccaccg cgatgaagga cacggcgtcg gacgccgcgc gcaagctggc cagcgcagtg
     1741 gtcaagacct cgctcgacgc ggcgccgcgt gccgcgaaag cgccgaaggc cgccgcgccg
     1801 gcggcgaaga agcgcaaacc cgcggcgaag aagaaggcgc cggccaagaa ggcggccagc
     1861 cgcgcctgac ccgccgatga cgcaccgatg aaccgcttcc tgcacggtca cgcgacccac
     1921 cccgactggc gcgtcgcgct cgcgctcgcc gcggcgcaga tcgacgcgca gcgcgagcag
     1981 cccggcacgg ccgcggcgcc gacgctcggc tgggtctacc tcaccgacgc cttggcggcg
     2041 cacgccgccg cgctgctggc cgaactgggc cggcgctggc ccggcgtcga atgggtcggc
     2101 gcgagcggcg tgggcatcgc cgccggcggc gtcgagtact tcgacgaacc cgcgctgtcg
     2161 ctgctgctga ccgacctcgc gcccgcgcac ttccgcgtgt tctcgggcgc gcggccgctg
     2221 gcggggttcg cggcccacac cgcgaacgtg cacgccgacc ccggcaccgc cgacctcgcc
     2281 gacctgatcc acgacatgag cgcgcgcacc gccaccggct acctgttcgg cgggctcgca
     2341 tcgggccgct cgcacccgct gcacgtcgcg ggcggcgtgc tctcgggcgg cctgtcgggg
     2401 gtcgcgttcg accgcagcgt gccgctggtc tcgcgcgtca cgcaaggctg ccagccggtc
     2461 ggcccgcagc gccgcgtgag cgcggccgag cgcaacgtgg tcgtcacgct cgacggc
//
LOCUS       DASRDG010000277         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_402741, whole genome shotgun sequence.
ACCESSION   DASRDG010000277 DASRDG010000000
VERSION     DASRDG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402741"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFA35_04605"
     CDS             complement(<1..459)
                     /locus_tag="VFA35_04605"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HZT55484.1"
                     /translation="MNDPTEPFAAPAPRRSIRSFVVRAGRMGPGQTRALAELGPRFVL
                     PYAAAPFDFAARFGRRAPQVLEIGFGMGDATAAIAQALPGTDFIGVEVHTPGVGALLK
                     QIGARGLTNLRLIQHDAVDVLEHMVAPASLAGVHVFFPDPWHKKKHNKRRL"
     gene            585..2063
                     /gene="dacB"
                     /locus_tag="VFA35_04610"
     CDS             585..2063
                     /gene="dacB"
                     /locus_tag="VFA35_04610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZT55485.1"
                     /translation="MSKLPSILPTVVCSALAALVLAATAAPRAAPAAANLPPAVASAI
                     RASGLPAQSFGLYAQEVIGDRTAFALNADQPYTMASTTKIVTSLAALDLLGPYYRWRT
                     SAFALGTLADGKLIGDLLIVGGGNAQLTSADLAKWFKRMHAQGLREIEGDIVLDRYAF
                     QLRPADHKGTPMQSAGEPRHVWPDAMTLDEGQLAVLVQPARGARAAAISLRPALSDVR
                     LDNRLTAGGGCSAAPSWRNERDGRATVVVQGSWDAGCGARSISFVPPPDSGVAARALP
                     AMWAATGGVLRGKVVSARVVPGVSPIPLGADGEPVKPLSFDRSKPLTELVRDINKVSD
                     NVGARNLMLSLSPGFPARPATLAGAQRTIDLWLRDQGLADGDIEVENGSGLSHTERAK
                     PRAMVQLLRKAWRAEQAQAFFDSLPIAGVDGTMKGRLVGSRAAGQAYLKTGTLNDTRA
                     LAGYVHGMSGKMYAVALMVNSADPAAGRPALDAMVAWLARNG"
     gene            2104..2177
                     /locus_tag="VFA35_04615"
     tRNA            2104..2177
                     /locus_tag="VFA35_04615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2136..2138,aa:Gly,seq:ccc)
     gene            2217..>2587
                     /locus_tag="VFA35_04620"
     CDS             2217..>2587
                     /locus_tag="VFA35_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55486.1"
                     /translation="MPPSDPDIAPPALQIVTQAALPAALADSPFGPFVLQPCAGRDAV
                     LARLAAGGCDALVLDDGADAIDLLAEAERDVAILVVAASPDPAAVLAWLARGAQDVLR
                     IDELCAASLPQRLRAAIERKRL"
BASE COUNT          348 a          934 c          952 g          353 t
ORIGIN      
        1 cagccggcgc ttgttgtgct tcttcttgtg ccacgggtcg gggaagaaca cgtgcacgcc
       61 ggccagcgag gccggcgcga ccatgtgctc gagcacgtcg accgcgtcgt gctggatcag
      121 gcgcaggttg gtgaggccgc gcgcgccgat ctgcttcagc agcgcgccga cgcccggcgt
      181 gtgcacctcg acgccgatga agtcggtgcc cggcagcgcc tgcgcgatcg cggcggtcgc
      241 gtcgcccatg ccgaagccga tctcgagcac ctgcggcgcg cggcggccga agcgcgcggc
      301 gaagtcgaac ggcgcggccg cgtagggcag cacgaagcgg ggccccagtt cggcgagcgc
      361 gcgcgtctgc cccggcccca tgcggccggc gcgcacgacg aagctgcgga tgctgcggcg
      421 cggtgccggt gcggcgaagg gttcggtggg gtcgttcatc ggtgtgcaag cgcggaaaat
      481 cggcaggctg gattgtgacg gcggccacgc aaacggccgc gcatgccgcg accacactgg
      541 accaggggtt caagcgccgc gcccggcctg ctagcctcgc ggccatgtcg aaactgccga
      601 gcatcctgcc caccgtcgtt tgcagcgcgc tggccgcgct tgtgctggcc gccaccgcgg
      661 caccgcgcgc cgcgccggcc gctgccaacc tgccgcccgc ggtcgcgagc gcgatccgcg
      721 cctcgggcct gccggcgcag agtttcgggc tgtacgcaca agaggtgatc ggcgaccgca
      781 ccgcgttcgc gctgaacgcc gaccagccct acacgatggc ttcgacgacc aagatcgtca
      841 cctcgctggc cgcgctcgac ttgctcgggc cgtactatcg ctggcgcacg tccgcgttcg
      901 cgctcggcac gctggccgac ggcaagctga tcggcgacct gctgatcgtc ggcggcggca
      961 acgcccagct cacctcggcc gacctggcga aatggttcaa gcgcatgcac gcgcaaggcc
     1021 tgcgcgagat cgagggcgac atcgtgctcg accgctacgc cttccagctc cgtccggccg
     1081 accacaaggg cacgccgatg cagtcggccg gcgagccgcg ccatgtgtgg cccgacgcga
     1141 tgacgctcga cgaaggccag ctggcggtgc tcgtccagcc cgcgcgcggc gcgcgggccg
     1201 ctgcgatctc gctgcggccc gcgctgagcg acgtgcggct cgacaaccgg ctcaccgcgg
     1261 gcggcggctg cagcgccgcg ccgagctggc gcaacgagcg tgacggccgc gcgaccgtgg
     1321 tggtgcaggg cagctgggac gcgggctgcg gcgcgcgcag catcagcttc gtgccgccgc
     1381 ccgacagcgg cgtcgccgcg cgcgcgctgc cggcgatgtg ggccgcgacc ggcggcgtgc
     1441 tgcgcggcaa ggtcgtttcg gcgcgggtcg tgccgggcgt cagcccgatc ccgctgggtg
     1501 cggacggcga gccggtcaag ccgctgtcgt tcgaccgctc gaagccgctg accgaactcg
     1561 tgcgcgatat caacaaggtc agcgacaacg tcggcgcgcg caacctgatg ctgtcgctgt
     1621 cgcccggctt cccggcgcgg ccggcgacgc tggcgggcgc gcagcgcacc atcgacctgt
     1681 ggctgcgcga ccagggcctg gccgacggcg acatcgaggt cgagaacggt tcgggcctgt
     1741 cgcacaccga gcgcgccaag ccgcgcgcga tggtgcagct gctgcgcaag gcctggcgcg
     1801 ccgagcaggc gcaggcgttc ttcgattcgc tgccgatcgc cggcgtcgac ggcacgatga
     1861 agggccggct ggtcggcagc cgagcggccg ggcaggcgta cctgaagacc ggcaccttga
     1921 acgacacgcg cgcgctcgcc ggctacgtgc acggcatgag cggcaagatg tacgcggtcg
     1981 cgctgatggt caacagcgcc gacccggcgg cggggcggcc ggcgctggat gcgatggtgg
     2041 cgtggctggc gaggaacggg tgagcgtgcc gccgcgacgg cgggctagaa tggcgcccga
     2101 tctgcgggtg tagttcaatg gcagaacggc agcttcccaa gcttcataca ggggttcgat
     2161 tcccctcacc cgctccagac aagacagata ctttcggcca gccctgttct gtggccatgc
     2221 ccccttcaga ccccgacatc gcaccccccg ccttgcagat cgtgacccag gcggcgttgc
     2281 cggctgcgct ggccgactcg cccttcgggc cgttcgtgct gcagccgtgc gccgggcgcg
     2341 acgccgtact cgcacgtctg gccgcgggcg gctgcgacgc actggtgctc gacgacggcg
     2401 ccgacgcgat cgacctcttg gccgaggccg agcgcgacgt cgcgatcctg gtcgttgcgg
     2461 cgtcgcccga ccccgccgcg gtgctcgctt ggctcgcgcg cggcgcgcag gacgtgctgc
     2521 gcatcgacga actctgcgcc gcctcgttgc cgcagcgcct gcgcgcggcg atcgagcgca
     2581 agcgcct
//
LOCUS       DASRDG010000278         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1425255, whole genome shotgun sequence.
ACCESSION   DASRDG010000278 DASRDG010000000
VERSION     DASRDG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425255"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFA35_04625"
     CDS             complement(<1..896)
                     /locus_tag="VFA35_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZT55487.1"
                     /translation="MSAQPSVKVALAQINATVGDLAGNGARIVEAARRAHAQGARLVL
                     TPELGLCGYPPEDLLLRPAFMLACAEALADCARALADLPGLFVVVGHPHQFGDRGDVR
                     SNSVSVPQRYNAASVISEGRVLATYCKRELPNYQVFDERRYFASGRDAGQGAVVFEVG
                     GLKFGLNICEDAWFDEPGVTAKAAGAQVLCVLNASPFHLGKLGEREQRMAQTAGALCM
                     PLLYAHLAGGQDEVVFDGASFALDAEGRVTARAPMFDEALTLVTFDGAVPHGPIAEVP
                     SIEAQAWAALVVGVRDYVNKNG"
     gene            907..2127
                     /locus_tag="VFA35_04630"
     CDS             907..2127
                     /locus_tag="VFA35_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT55488.1"
                     /translation="MMKNAGVRERDAQSEDAGSKASNDYVIRVFDDPAAIDAAQWNAL
                     LDAQPSSTPFMRHQYLLALHQSASAVARTGWLPQFLAVFDGDALIAACPLYLKDHSYG
                     EYVFDWAWADAYQRHGLHYYPKLLDAVPFTPVPGPRLLAADPAGRRMLLRAMQQLARN
                     AKLSSAHLLFLDEADQQAARMEGWMMRSTVQFHWTQREAAPYADFADLLASLQRDKRK
                     KIQQERRKVADAGVVFTELQGAQITPDDWDFFYRCYAQTYREHRSTPYLTRDFFARMA
                     TTMPENWLLFVACAGGERIAASLICIDPARRTAFGRYWGATRHIACLHFEACYYRPLA
                     WCIANGYQRFEGGAQGEHKMARGLLPVQTWSAHWLAHPQFAQAVGDFLEREGAGVAGY
                     ISELNDRRPFKAAG"
     gene            complement(2147..2683)
                     /gene="ppa"
                     /locus_tag="VFA35_04635"
     CDS             complement(2147..2683)
                     /gene="ppa"
                     /locus_tag="VFA35_04635"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZT55489.1"
                     /translation="MSLHNVTPGAKAPDQFNVIIEIPMNADPIKYEVDKESGALFVDR
                     FMTTAMHYPCNYGYIPQTLSDDGDPVDVLVITPYALTPGVVVTCRALGVLKMEDEAGG
                     DAKLLAVPIDKVLPIYTHWQKPDDINQMRLKAIQHFFEHYKDLEPGKWVKVQGWEGPE
                     SAKAEIVSGIAAYKKIAK"
BASE COUNT          481 a         1054 c          954 g          474 t
ORIGIN      
        1 aagccgttct tgttcacata gtcgcgcacg ccgaccacca gcgcagccca cgcctgcgcc
       61 tcgatcgaag gcacctcggc gatcgggccg tgcggcaccg cgccgtcgaa cgtgaccagc
      121 gtcagcgcct cgtcgaacat cggtgcgcgc gcggtcacgc ggccctcggc atcgagcgcg
      181 aacgaggccc catcgaacac gacctcgtcc tgcccgcccg ccaggtgcgc gtacagcagc
      241 ggcatgcaca gcgcgcccgc ggtctgcgcc atgcgttgct cgcgctcgcc cagcttgccg
      301 aggtggaacg gcgatgcgtt cagcacgcac agcacctgcg cgccggcggc cttcgcggtg
      361 acgccgggct cgtcgaacca cgcgtcttcg cagatgttca ggccgaactt caggccaccc
      421 acctcgaaca cgaccgcgcc ctgtcccgca tcgcggcccg acgcgaaata gcggcgctcg
      481 tcgaacacct ggtagttcgg caactcgcgc ttgcagtacg tcgcgagcac ccggccttcg
      541 ctgatcaccg aggccgcgtt gtagcgctgc ggcaccgaca ccgagttcga cctaacatcg
      601 ccccgatcac cgaactgatg cggatgcccc accaccacga acaagcccgg caagtcggcc
      661 aaggcgcgcg cgcagtcggc cagcgcctcg gcgcacgcca gcatgaaggc ggggcgcagc
      721 agcaggtctt cgggcgggta gccgcacagg ccgagttcgg gcgtgagcac gagcctggcg
      781 ccctgcgcgt gagcacggcg tgccgcctcg acgatgcgcg cgccgttgcc ggccagatcg
      841 ccgaccgtgg cgttgatctg cgccagggcc actttgaccg aaggttgtgc tgacatgggt
      901 ggcgaaatga tgaagaacgc aggcgtgcgc gagcgggatg cacagagcga agacgcgggc
      961 tcgaaggcct cgaacgatta tgtcattcgg gtcttcgacg accccgccgc gatcgacgcc
     1021 gcgcagtgga acgccctgct cgacgcgcag ccgagcagta cgccgttcat gcgccaccaa
     1081 tacctgctgg cgctgcatca gtcggcgagc gcggtcgcgc ggaccggctg gttgccgcag
     1141 ttcctcgccg tgttcgacgg cgacgcactg atcgcggcct gcccgctgta cctcaaagac
     1201 cactcctacg gcgagtacgt gttcgactgg gcctgggccg acgcctacca gcgccatggt
     1261 ctgcactact acccgaagct gctcgacgcg gtgccgttca ccccggtgcc cggcccccgc
     1321 ctgctggcag ccgacccggc gggccggcgc atgctgctgc gcgcgatgca gcagctggcg
     1381 cgcaacgcga agctctcgtc ggcgcacctg ctgttcctcg acgaggccga ccagcaggcc
     1441 gcgcgcatgg agggctggat gatgcgcagc accgtccagt tccactggac ccagcgcgag
     1501 gcggcgccct acgccgactt cgccgacctg ctcgcgagcc tgcagcgcga caagcgcaag
     1561 aagatccagc aggagcgccg caaggtcgcc gacgcgggcg tcgtgttcac cgaactgcaa
     1621 ggcgcgcaga tcacgcccga cgactgggac ttcttctacc gctgctacgc gcagacctac
     1681 cgcgagcacc gttcgacgcc ttatctgacg cgcgacttct tcgcgcgcat ggcgaccacg
     1741 atgccggaga actggctgct cttcgtcgcg tgcgcaggcg gcgagcgcat cgccgcatcg
     1801 ctgatctgca tcgaccccgc gcgccgcacc gcgttcggcc gctactgggg cgcgacccga
     1861 cacatcgcgt gcctgcactt cgaggcctgc tactaccggc cgctcgcctg gtgcatcgcg
     1921 aacggctacc agcgcttcga aggcggcgcg cagggcgagc acaagatggc gcgcggcctg
     1981 ctgccggtgc agacctggtc ggcgcactgg ctcgcgcacc cgcagttcgc ccaggcggtc
     2041 ggtgacttcc tcgaacgcga gggcgcaggc gtggccggct acatcagcga gctcaacgac
     2101 cggcggccat tcaaggctgc cgggtgatca ggtctcagct cggcggctac ttcgcgatct
     2161 tcttgtacgc agcgatgccc gagacgatct cggccttcgc cgactccggg ccttcccagc
     2221 cctgcacctt gacccacttg ccgggttcca ggtccttgta gtgctcgaag aaatgctgga
     2281 tcgccttcag gcgcatctgg ttgatatcgt cgggtttctg ccagtgcgtg tagattggca
     2341 gcaccttgtc gatcggcacc gcgagcagct tggcatcgcc accggcctcg tcttccatct
     2401 tcagcacccc gagggcgcgg caggtcacga cgacgccggg cgtcagcgcg tacggcgtga
     2461 tcacgagcac gtcgaccggg tcgccgtcgt ccgacagcgt ctgcgggatg tagccgtaat
     2521 tgcacgggta gtgcatcgcc gtcgtcatga agcggtcgac gaacagcgcg ccgctttcct
     2581 tgtcgacctc gtatttgatc gggtcggcgt tcatcgggat ctcgatgatg acgttgaact
     2641 ggtcgggcgc cttggcgccg ggggtcacgt tgtgcaggct catggtggct ctttggtacg
     2701 cgggggatgc cgatcttaag gccacggccg ggccgccaaa ccgcgcagca accgctggaa
     2761 aacaccatcc cggcgacgtt gctttgtcca gacccatctg gcagcatgcc gcgaggccgg
     2821 gccacggata aacgcacacg ctgcgtgacg ctcgaagtcg gcctcgaacc ccccacgcat
     2881 tcgacaagga aggacttccc ttgatcagca catcgatggc gctctacttt gcattggcct
     2941 gcggcctcgc cgcagtcgtc tac
//
LOCUS       DASRDG010000279         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_613790, whole genome shotgun sequence.
ACCESSION   DASRDG010000279 DASRDG010000000
VERSION     DASRDG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613790"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..574)
                     /locus_tag="VFA35_04640"
     CDS             complement(167..574)
                     /locus_tag="VFA35_04640"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZT55490.1"
                     /translation="MTELEQARLLRAARDAQTHAYAPYSHFAVGAAVLDENGRIHAGC
                     NVENAAYPEGVCAEAGALSAMVLAGGRRVRALAVVGDGAGLVTPCGGCRQKLREFAAA
                     DTPVLVGDREQLRAEFTLAQLLPSSFGPEHLAP"
     gene            complement(582..1562)
                     /locus_tag="VFA35_04645"
     CDS             complement(582..1562)
                     /locus_tag="VFA35_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55491.1"
                     /translation="MDEALVGALIASTLRVATPLILCALAGLLAERSGVVDIGLEGKM
                     LFAAFAAAAAGAAFDSTAIALLLACGVAILLSWLHGFATVSHSGDQVVSGMAINIIAA
                     GLTVVLGIAWFHQGGQTTPVSDAVRLHALWPGAQAALQGVPWVGGLLGAALGEGLLSH
                     TALVYVALALVGGVWWLVYRTRFGLRLRAVGENPHMVDAAGVSVIRLRYAALTLNGVL
                     CGLAGSYLVLAQNAAFAPNMTAGRGYMALAALIFGNWRPVPALLACLLFGFLDAAAIR
                     LQGVSLPGIGEVPVQAIQALPYLLTVILLAGFIGRTVAPKALGLPYVKER"
     gene            complement(1555..>2249)
                     /locus_tag="VFA35_04650"
     CDS             complement(1555..>2249)
                     /locus_tag="VFA35_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55492.1"
                     /translation="NFLASTLLVYLLVNRLKAPGGMSVESVPFGPAAHLPGMHEALAW
                     FGVEWPSSPLNLSLFLALAAAVGVWAFLWKSRAGYRLRAVGSSEGAAAYAGIRPKRQV
                     ILAMALSGALAGMVGMNEIAGVNGKLLLDFVGGAGFTGIAVAVMGRNHPLGIVLASLL
                     FGALFQGGAEVSFEIPGFSRDMVVTLQGFIVLFSGAMAYVIAPWLARLLRLGAPRRVA
                     GAAAAAGGASNG"
BASE COUNT          359 a          862 c          781 g          247 t
ORIGIN      
        1 cgcggaaagc cctcgagctg gtcgtaaccg atgcgcacgg gctcggcgac gcgctcggtc
       61 agcgcgcccc agcccgagcc gagcacgacg gcgacgcgcg gccgcgcgtt cggcgcgagc
      121 gcgcgcacct gctgcgcggc gggcaagtgg gtctggctcg cgctcgtcat ggcgccaggt
      181 gctccgggcc gaagctcgac ggcagcaact gcgcgagcgt gaactcggcg cgcagctgct
      241 cgcggtcgcc gaccagcacc ggggtgtcgg ccgcggcgaa ctcgcgcagc ttctggcggc
      301 agccgccgca cggcgtcacg agcccggcac cgtcgccgac gaccgcgagc gcgcgcaccc
      361 gccggccgcc ggccagcacc atcgcgctga gcgcgccggc ctcggcgcat accccttccg
      421 ggtaggcggc gttctcgaca ttgcagccgg cgtggatccg gccgttttca tcgagcaccg
      481 cggcgccgac cgcgaagtgc gagtacggcg catacgcatg cgtctgcgcg tcgcgcgcgg
      541 cgcgcagcag ccgggcttgt tcgagttctg tcatcttttt ctcaacgctc tttgacatag
      601 ggcaggccca aggctttcgg cgcgaccgtg cggccgatga agcccgccag caggatcacc
      661 gtcagcaagt acggcagcgc ctggatcgcc tgcaccggca cctcgccgat gccgggcagg
      721 ctcacgccct gcaggcggat cgccgcggca tcgagaaagc cgaacagcag gcaggcgagc
      781 agcgcaggca ccggccgcca gttgccgaag atcagcgcgg ccagcgccat gtagccgcgc
      841 ccggcggtca tgttcggcgc gaacgcggcg ttctgcgcca gcaccaggta gctgccggcc
      901 aggccgcaca gcacaccgtt gagcgtgagc gcggcgtagc gcaggcggat caccgacacg
      961 ccggcggcgt cgaccatgtg cgggttctcg ccgaccgcgc gcagtcgcag gccgaagcgc
     1021 gtgcgataca ccagccacca gacgccgccg accagcgcga gcgccacgta gaccagcgcc
     1081 gtgtggctca gcagcccttc gccgagcgcc gcgccgagca gcccgccgac ccacggcacg
     1141 ccttgcagcg ccgcttgcgc gccgggccac agcgcgtgca gccgcaccgc gtcgctgacc
     1201 ggcgtggtct ggccgccctg gtgaaaccac gcgatcccga gcacgacggt gaggccggcc
     1261 gcgatgatgt tgatcgccat ccccgagacg acctggtcgc cgctgtggct gaccgtcgcg
     1321 aagccgtgca gccaggacag caggatcgcc accccgcagg ccagcagcag cgcgatcgcg
     1381 gtcgagtcga acgcggcgcc ggcagcggcc gccgcgaagg ccgcgaacag catcttgccc
     1441 tccagcccga tgtcgaccac gcccgagcgc tcggccagca gcccggcgag cgcgcacagg
     1501 atcagcggcg tggccacgcg cagcgtggac gcgatcagcg caccgaccag cgcctcatcc
     1561 attcgacgct ccgccggcgg cggccgcggc gcccgccacg cggcgcggcg cgcccaggcg
     1621 cagcagccgc gccagccacg gcgcgatcac gtaggccatc gcgcccgaga acagcacgat
     1681 gaagccctgc agcgtgacca ccatgtcgcg gctgaacccc gggatctcga agctcacctc
     1741 ggcgccgccc tggaacagcg cgccgaacag caggctcgcg agcacgatgc ccagcggatg
     1801 gttgcgcccc atcaccgcga ccgcgatgcc ggtgaagccg gcgccgccga cgaagtcgag
     1861 cagcagcttg ccgttgacgc cggcgatctc gttcatgccg accatgccgg cgagtgcgcc
     1921 cgacagcgcc atcgccagga tcacctggcg cttcgggcgg atgccggcgt aagccgccgc
     1981 gccctcgctc gaaccgaccg cgcgcagccg gtagccggcg cggctcttcc acaggaacgc
     2041 ccagacgccg accgccgcgg cgagcgccag gaacaggctg aggttcagcg gcgacgacgg
     2101 ccactcgacg ccgaaccacg cgagcgcctc gtgcatgccc ggcaggtgcg cggccggccc
     2161 gaacggcacg ctctcgaccg acatgccgcc cggcgccttc agccggttca cgagcaggta
     2221 gaccagcaag gtgctggcga gaaagttga
//
LOCUS       DASRDG010000280         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1648656, whole genome shotgun sequence.
ACCESSION   DASRDG010000280 DASRDG010000000
VERSION     DASRDG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648656"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1461)
                     /locus_tag="VFA35_04655"
     CDS             complement(97..1461)
                     /locus_tag="VFA35_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZT55493.1"
                     /translation="MTRKPDRRFETSIQIRAARDAVWQALTEAREIARWFAPQAEVDP
                     RVGGRMLWEWGDAHSWPQTIEAFEPGRHLRTRYDSGVADGQGGKRPLFVDFHLEGDRG
                     VTTLRVTHSGFGPEADFDGEFDGISNGWPVELRSLRLYLEQHRGVDRSLVWALRRTAA
                     PPEQVWARLVGPDGLAALDLAATREGERFTVAVPGAGALSGTALLSRPGREFTGIADD
                     FGSAWLRIYCEPHGDGTHVWLWLASYGQPEAKVKTFRAAFERLLDRVIAPSASPPSAS
                     RRAAPAPLTAEWRALLERTPPMLDAWLRTLPAAWLECDEGPSTWCPLQVLGHLIEGER
                     SDWLPRVRHLLEHGERVAFPPFDREAGQRRGPRTVAALLDEFAAARRQSLREIDDLRL
                     RAADLERKGRHPEFGAVTLGQHLATWAAHDLTHILQIARAMGRSLEGAVGPWRAYLRV
                     VREP"
     gene            complement(1470..2066)
                     /locus_tag="VFA35_04660"
     CDS             complement(1470..2066)
                     /locus_tag="VFA35_04660"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZT55494.1"
                     /translation="MVSAFTHAAAAVAIGTLVPRERLPRRAWLVGAVAAVLPDLDYFL
                     HRSGFWGEHPYGHRGFTHSLTFAAALGGASTALAAWRRWPGGRWWWFAYLTVCTASHG
                     VLDAFTNGGEGIAFLWPFTNARWHFPWTPIEVAPLSIDGFFTARGAEVFASELLWVWL
                     PAAALVLAGAVARRHRQTRLSVVDSSPTNDTVGAIDRP"
BASE COUNT          297 a          787 c          806 g          272 t
ORIGIN      
        1 cgaggatctg caggcgcgtc gggtgcagca gcacctgcgc gcggttgcgg tccgcgacca
       61 cctcgacggc ctcggtcggg ggcgcggcgc ggcggctcac ggttcgcgca ccacgcgcag
      121 gtacgcgcgc caaggaccga ctgcgccttc gaggctgcgg cccatcgcgc gcgcgatctg
      181 caggatgtgc gtgaggtcgt gcgccgccca ggtggcgagg tgctggccca gcgtgaccgc
      241 gccgaactcg gggtggcggc ccttgcgctc gagatccgcg gcgcgcagac gcaggtcgtc
      301 gatctcgcgc aggctctgcc gccgcgcggc cgcgaactcg tcgagcagcg ccgcgaccgt
      361 gcgcggcccg cggcgctgac ccgcctcgcg gtcgaacggc gggaacgcga cgcgctcgcc
      421 gtgctcgagc aggtggcgca cgcgcggcag ccagtcgctg cgttcgccct cgatcagatg
      481 gccgagcacc tgcagcggac accaggtcga cgggccctcg tcgcactcga gccacgcggc
      541 tggcagcgtg cgcagccacg cgtcgagcat cggcggcgtg cgctcgagca gcgcgcgcca
      601 ttccgcggtc agcggcgcgg gcgccgcgcg gcgcgatgcg gacggcggcg atgcggacgg
      661 cgcgatgacg cggtcgagca gccgctcgaa cgcggcgcgg aaggtcttca ccttcgcctc
      721 gggctggcca tagctcgcga gccacagcca gacgtgcgtg ccgtcgccat ggggctcgca
      781 gtagatgcgc agccacgcgc tgccgaagtc gtcggcgatg ccggtgaact cgcgcccggg
      841 ccgggacagc agcgcggtgc cggagagcgc gccggcgccg ggcaccgcga ccgtgaaccg
      901 ctcgccctcg cgagttgcgg cgaggtccag ggccgcgagg ccgtctggtc cgacgagccg
      961 tgcccagacc tgctcgggag gcgcggccgt cctgcgcagc gcccagacga ggctgcgatc
     1021 cacgccgcgg tgctgctcga ggtagagccg caggctgcgc agctcgacgg gccagccgtt
     1081 gctgatgccg tcgaactcgc cgtcgaagtc ggcttcgggc ccgaagcccg agtgcgtgac
     1141 gcgcagcgtc gtcacgccgc gatcgccttc gaggtggaag tcgacgaaca gcgggcgctt
     1201 gccgccctgg ccgtcggcga cgcccgagtc gtagcgggtg cgcaggtggc ggcccggctc
     1261 gaacgcctcg atcgtctgcg gccaggagtg ggcgtcgccc cactcccaca gcatgcggcc
     1321 gccgacgcgc ggatcgactt cggcctgcgg ggcgaaccag cgcgcgatct ccctggcttc
     1381 ggtgagcgcc tgccacacgg cgtcgcgcgc ggcgcggatc tggatcgagg tctcgaaacg
     1441 gcggtcgggc ttgcgggtca tggtcggtcc tatggtcggt cgatcgcgcc gacagtatcg
     1501 tttgtcggtg agctgtcaac caccgacagt ctggtttgtc ggtggcggcg cgcgaccgcg
     1561 ccggccagca ccagcgccgc ggccgggagc cacacccaca gcagctcgct ggcgaacacc
     1621 tcggcgccgc gcgccgtgaa gaaaccgtcg atgctcagcg gcgcgacctc gatcggcgtc
     1681 cacgggaagt gccagcgcgc gttcgtgaac ggccagagga acgcgatgcc ttcgccgccg
     1741 ttggtgaacg cgtcgagcac gccgtgcgac gcggtgcaca ccgtgaggta ggcgaaccac
     1801 caccagcgtc cgcccggcca gcgccgccac gccgccaatg cggtcgacgc gccgccgagc
     1861 gccgccgcga aggtcagcga atgcgtgaag ccgcggtgcc cgtacggatg ttcgccccag
     1921 aacccgctgc gatgcaggaa gtagtcgagg tcgggcagca ccgccgcgac cgcgcccacg
     1981 agccatgcgc ggcgcggcag ccgctcgcgc gggaccagcg tgccgatcgc gaccgccgcc
     2041 gccgcgtgcg tgaacgcgga gaccatcagc cggcctcgcc gcggtaccag gccagcaggt
     2101 cctcgatcac cttcaccgcc ttgccgtgga agtggttgtt ggcgaccacc agcgtcgtct
     2161 tc
//
LOCUS       DASRDG010000281         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_403135, whole genome shotgun sequence.
ACCESSION   DASRDG010000281 DASRDG010000000
VERSION     DASRDG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403135"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFA35_04665"
     CDS             <1..753
                     /locus_tag="VFA35_04665"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HZT55495.1"
                     /translation="DNGLLLRDGFASDGLAALTERFSAALLGFGYPRCPGNIMLSNPR
                     WCQPLGEFKQTLREWMFAGAADGVMNLAIFVDAAAVAGDAGLLRQARRFVDDFLVDDD
                     AFFARFASAADQFGDGSGWGSLLSGVRGKDEQVVDLKKLGSFPIVHGVRTLALQHHVG
                     ELGTAARLQVLAAQHHLPEPMARDLRDALHFFMRLKLDLHLRQRSSGVAADNLVRAAT
                     LGALERAGLHEALAIAKRFRQHLRRHYRFDVL"
     gene            779..976
                     /locus_tag="VFA35_04670"
     CDS             779..976
                     /locus_tag="VFA35_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55496.1"
                     /translation="MHQGLGRQRLIVLFVAGWLLLNFPLLALWDRDATVWGLPLFPAA
                     LFMLWAVLVVLLGLIAERMPD"
     gene            980..>2561
                     /locus_tag="VFA35_04675"
     CDS             980..>2561
                     /locus_tag="VFA35_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZT55497.1"
                     /translation="MLSAPLVVGASFAYLLLLFAVAAFGDARARQGRSVIGNAWVYAL
                     SMAVYCTAWTYFGSVGRAASAGVWFLPIYLGPTLAMALAWAVLRKMIRIAKAYRITSI
                     ADFIASRYGKSPLLAGTVTLITVVGIVPYIALQLKAVSSGYALLTTELGTPVAAPLSW
                     WRDSTFYFALALAGFTCAFGARHLDSAEKHEGMVAAIAFESVVKLLAFVAVGAFVTWG
                     VFDGLTDLFARARAVPELHKLLNLSQSPGQKFAYEQWFALTLLSMLSVIFLPRQFQMM
                     VVENVAEQHVKRAAWVFPLYLLLINLFVLPIALGGLLHFGAGRMDADAFVLSLPLAHG
                     HGGLALLVFVGGLSAATGMVIVEAIAVSTMVCNDLVMPMLLRTRRFGARAGGDLSRLL
                     LGIRRAAIVLILLLGYAYFAIAGEAYALVSIGLISFAAVAQFAPAMLAGLYWKGGTRL
                     GAFAGLLLGFALWAYTLMLPSVAKSGWLAGDVGVGFLRDGPWGVTLLKPEQLLGLSGL
                     DNLTHALFWSLLANVVAFV"
BASE COUNT          303 a          882 c          901 g          475 t
ORIGIN      
        1 gacaacgggc tgctgttgcg cgacggcttc gcctccgatg gcctggccgc cctgaccgaa
       61 cgtttcagcg ctgcgctgct gggcttcggc tacccgcgct gcccgggcaa catcatgctg
      121 agcaacccgc ggtggtgcca gccgctcggc gaattcaagc agaccttgcg cgaatggatg
      181 ttcgccggcg cggccgacgg cgtgatgaac ctggcgatct tcgtcgacgc cgccgcggtc
      241 gccggcgatg ccggcctgct gcgccaggcg cgccgcttcg tcgacgactt cctggtcgac
      301 gacgatgcct tcttcgcccg tttcgccagc gcggccgacc agttcggcga cggcagcggc
      361 tggggcagcc tgctcagcgg cgtgcgcggc aaggacgagc aggtcgtcga cctgaagaag
      421 ctcggcagct tcccgatcgt gcacggcgtt cgcaccctgg cgctgcagca tcatgtgggc
      481 gaactcggca cggcggcgcg gctgcaggtg ctggccgcgc agcaccacct gcccgagccg
      541 atggcgcgcg acctgcgcga cgcgctgcac ttcttcatgc ggctcaagct cgacctccac
      601 ctgcgccagc gcagcagcgg cgtggcggcc gacaacctgg tgcgcgccgc gacgctgggc
      661 gcgctcgagc gcgccgggtt gcacgaggcg ctggccatcg ccaagcgctt ccggcagcat
      721 ctgcgccggc actaccgatt cgacgtgctg tgagcgcacg cgcgacaagg agacccgaat
      781 gcaccaaggt ctgggccgcc agcggctgat cgtcctgttc gtcgccggct ggctgctgct
      841 gaattttccg ttgttggcgc tgtgggaccg tgacgcgacc gtttgggggc tgccgctgtt
      901 cccggccgcg ctgttcatgc tctgggcggt gctggtcgtg ctgctcggcc tgatcgcgga
      961 gcgcatgccg gactgaacca tgttgtcggc cccgctcgtc gtcggcgcct cgttcgccta
     1021 cctgttgctg ctgttcgcgg tggccgcgtt cggcgatgcg cgcgcgcgcc aggggcgctc
     1081 ggtcatcggc aacgcctggg tctacgcgct gtcgatggcg gtgtactgca cggcctggac
     1141 gtatttcggc agcgtcggcc gcgccgccag cgctggggtc tggttcctgc cgatctacct
     1201 cggcccgacc ctggcgatgg cgctggcgtg ggcggtgctg cgcaagatga tccgcattgc
     1261 caaggcctac cgcatcacct cgatcgccga cttcatcgcg agccgctacg gcaagagccc
     1321 gctgctcgcc ggcacggtga cgctgatcac cgtggtcggc atcgtgccct acatcgcgct
     1381 gcaactgaag gcggtgtcga gcggctatgc gctgctgacc accgagctgg gcacgccggt
     1441 ggccgcgccg ctgagctggt ggcgcgacag cacgttctat ttcgcgctcg cgctcgccgg
     1501 cttcacgtgt gcgttcggcg cgcgccacct cgacagcgcc gaaaagcacg aaggcatggt
     1561 cgcggcgatc gcgttcgagt cggtcgtcaa gctgctggcg ttcgtcgccg tcggcgcgtt
     1621 cgtgacctgg ggcgtgttcg acggcctgac cgacctgttc gcgcgggcgc gggccgttcc
     1681 cgagctgcac aagctgctca acctgtcgca gtcgccgggc cagaagttcg cctacgaaca
     1741 atggttcgcg ctgaccctgc tgtcgatgct gtcggtgatc ttcctgccgc gccagttcca
     1801 gatgatggtg gtcgagaacg tcgccgagca gcacgtgaag cgcgctgcct gggtgttccc
     1861 gctttacctg ctgctgatca acctgttcgt gctgccgatc gcgctcggcg gcctgctgca
     1921 cttcggcgcc gggcggatgg acgccgatgc cttcgtgctc tcgctgccgc tcgcgcacgg
     1981 gcacggcggc ctggcgctgc tggtcttcgt cggcgggctg tcggccgcga ccggcatggt
     2041 gatcgtcgag gcgatcgcgg tgtcgaccat ggtctgcaac gacctcgtga tgccgatgct
     2101 gctgcgcacg cggcgcttcg gtgcgcgtgc cggcggcgac ctgagccggc tgctgctggg
     2161 catccgccgc gccgcgatcg ttctgatcct gctgctcggc tacgcctact tcgcgatcgc
     2221 cggcgaggcg tatgcgctgg tcagcatcgg cctcatcagc tttgccgcgg tggcacagtt
     2281 cgcgccggcg atgctggccg ggctgtactg gaaaggcggc acgcgcctgg gcgcgttcgc
     2341 cgggctgctg ctcggctttg cgctgtgggc ctacacgctg atgctgccgt cggtggccaa
     2401 gtcgggctgg ctcgcgggcg atgtcggggt cgggtttctg cgcgacggcc cctggggcgt
     2461 cacgctgctc aagcccgagc aactgctcgg cctgagcggc ctggacaacc tgacccacgc
     2521 gctgttctgg agtctgctgg ccaacgtcgt cgccttcgtc g
//
LOCUS       DASRDG010000282         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_156164, whole genome shotgun sequence.
ACCESSION   DASRDG010000282 DASRDG010000000
VERSION     DASRDG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156164"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFA35_04680"
     CDS             <1..223
                     /locus_tag="VFA35_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZT55498.1"
                     /translation="CAQCHGTDGRAPPGSAVPALAGMPAAYLAEQMQAFKAGTRPGTV
                     MPQLAKGYSDAQIAQLAAYFAQPLTGGKP"
     gene            220..1506
                     /locus_tag="VFA35_04685"
     CDS             220..1506
                     /locus_tag="VFA35_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZT55499.1"
                     /translation="MKRRDVLRLAGAATGLGTLGLAGCAMNAIGTPDRARVLVVGGGF
                     GGATAAKYVRLLSKDKIDVVLVEPEAAFVSCPLSNLVLGGYRTLTEITRPYDTLGAAH
                     GVRVVRDMVASIDPVRRIAVLASGGTLRYDKLVLSPGVEMMFDGVAGLGAASRQGRIL
                     QAWKAGPETLALRRQLEAMPDGGIFAIAIPEAPYRCPPGPYERASVIAGYFKAAKPKS
                     KVLILDANDDVTSKGALFKKVWAESYKGMVEYRPQHKATAVDATTNTVKFEVQDDVRA
                     DVLNVLPPMRAGRVAVQTGLATSNARWCKVDYLNFESTEAQHIHVIGDAIQIAPLMPK
                     SGHMASSHAKVAAAAIVAELSGWELNPAPMLTNTCYSFVDDKRVVHVASVHGYVAAEK
                     TFKTVAGSGGLSAAPNELEGALALSWARSIWADMLS"
     gene            1716..3551
                     /locus_tag="VFA35_04690"
     CDS             1716..3551
                     /locus_tag="VFA35_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT55500.1"
                     /translation="MQATDIHAPDYFHRVVDCQWACPAHTPVPEYIRLIAAGRYTDAY
                     MVNWASNVFPGILGRTCDRPCEPACRRGRVEQAQAAKPEPVAICRLKRVAADFKDTNI
                     AERLPRAPQVMNGKRVACVGAGPAALTVARDLAPLGYAVTVFDADPKAGGMIRTQIPR
                     FRLPEAVIDEETGYVLRLGVEFRAGTRIDSLAALLEDKTNGGWDAVFIGSGAPRGRDL
                     KIPGRDEAAAHIHIGIEWLASVSFGHVTQIGRRVIVLGGGNTAMDCCRSAKRLGGDDV
                     KVIVRSPFDEMRASPWEKEDAAHEGIAILDCLVPTAFVHEGGKLTGMRFEKVRALRDA
                     AGRRQLVPTGEPEHVYACDEVLVAVGQENAFPWIERDAGIAFDAHGMPVLDAVTLQSS
                     RPNVFFGGDAALGPKNIIWAVAQGHAAAISIDKFLHGENVAVRPAPGVTLVSQKMGIH
                     EWSYDNAVTSDTRHAVPWREVGKALRDIKVEVELGFDAELAWNEAQRCLNCDVQTVFN
                     TRQCIECDACVDICPTDCISFTANGIEADLRTRLRAPARNIDQALYVSDELKTGRVMV
                     KDEDVCLHCGLCAERCPTGAWDMQKFLIDMTHAGPACRNPKRKAA"
BASE COUNT          589 a         1264 c         1258 g          478 t
ORIGIN      
        1 ctgcgcccag tgccacggca ccgacggccg tgcgccgccc ggctcggccg tgcccgcgct
       61 cgccggcatg ccggcggcct acctggccga gcagatgcag gcgttcaagg ccggcacccg
      121 ccccggcacc gtgatgccgc agctcgccaa gggctacagc gacgcgcaga tcgcgcaact
      181 ggccgcgtac ttcgcgcagc cgctcaccgg aggcaagcca tgaagcgccg cgacgtactc
      241 aggctcgccg gagccgccac cggcctgggc acgctcggcc tcgccggctg cgcgatgaac
      301 gcgatcggca cgcccgaccg cgcacgcgtg ctcgtcgtcg gcggcggctt cggcggcgcg
      361 accgcggcga agtacgtgcg cctgctgtca aaggacaaga tcgacgtggt gctggtcgaa
      421 cccgaggccg cgttcgtctc gtgcccgctg tcgaacctgg tgctcggcgg ctaccgcacg
      481 ctgaccgaga tcacgcggcc ctacgacacg ctcggcgcgg cgcacggcgt gcgcgtggtg
      541 cgcgacatgg tcgcgagcat cgacccggtc aggcgcatcg ccgtgctcgc gtcgggcggc
      601 accctgcgct acgacaagct ggtgctctcg cccggggtcg agatgatgtt cgacggcgtc
      661 gccgggctcg gcgccgcgag ccggcagggc cgcatcctgc aggcctggaa ggccggcccc
      721 gagacgctcg cactgcgccg ccagctcgag gcgatgcccg acggcggcat cttcgcgatc
      781 gcgatccccg aggcgccgta ccgctgcccg ccggggcctt acgagcgcgc gtcggtgatc
      841 gccggctact tcaaggccgc gaagccgaag tcgaaggtgc tgatcctcga cgcgaacgac
      901 gacgtgacga gcaagggcgc gctgttcaag aaggtctggg ccgagtcgta caagggcatg
      961 gtcgagtacc ggccgcagca caaggcgacc gcggtcgacg ccacgaccaa caccgtgaag
     1021 ttcgaggtcc aggacgacgt gcgcgccgac gtactgaacg tgctgccgcc gatgcgcgcc
     1081 ggcagggtcg cggtgcagac cggactggcg acgtcgaacg cgcgctggtg caaggtcgac
     1141 tacctgaact tcgagtcgac cgaagcccag cacatccatg tgatcggcga cgcgatccag
     1201 atcgcacctt tgatgcccaa gagcggccac atggcgagca gccatgccaa ggtggcagcc
     1261 gcggcgatcg tcgccgagct gagcggctgg gagttgaatc ccgcgccgat gctgaccaac
     1321 acctgctaca gcttcgtcga cgacaagcgc gtcgtgcacg tggccagcgt gcacggctac
     1381 gtggcggccg agaagacctt caaaaccgtg gccggctcgg gcgggctgtc ggccgcgccg
     1441 aacgagctgg aaggcgctct ggcgctgagc tgggcgcgct cgatctgggc cgacatgctg
     1501 agctgacgcc ggcccccgcc gggcgcgcgt gacggtactc acgcgaatac cgcgcggcgt
     1561 gccttgccat ggcgcaaacc ctcgctgaca gcgccggggc gcaggcctag catgctcgac
     1621 atcgcaccta cgcaacagcg gcgccacagc gccgcaacag cgcgtcggcc cccgcgtggc
     1681 acgcccaccg gcacgagcgc gcggagagaa acggcttgca agcaaccgat atccacgcac
     1741 ccgactactt ccacagagtc gtcgactgcc agtgggcgtg cccggcccac accccggttc
     1801 ccgaatacat ccgcctgatc gcggcgggcc gctacaccga tgcctacatg gtcaactggg
     1861 cctcgaacgt gttccccggc atcctcggcc gcacctgcga ccggccctgc gaaccggcct
     1921 gccggcgcgg ccgggtcgag caggcgcagg cggccaagcc cgagccggtc gcgatctgcc
     1981 ggctgaagcg cgttgccgcc gacttcaagg acaccaacat cgccgagcgc cttccgcgcg
     2041 cgccgcaggt gatgaacggc aagcgcgtcg cctgcgtcgg cgccgggccg gcggcgctga
     2101 cggtggcgcg cgacctggcg ccgctgggct acgcggtcac ggtgttcgat gccgacccga
     2161 aggccggcgg gatgatccgc acccagattc cgcgctttcg cctgcccgag gcggtgatcg
     2221 acgaagagac cggctacgtg ctgaggctgg gcgtcgagtt tcgcgccggc acacggatcg
     2281 actcgctggc cgcgctgctg gaggacaaga cgaacggcgg ctgggacgcg gtcttcatcg
     2341 gctcgggcgc gccgcgtggc cgcgacctga agatccccgg ccgcgatgag gccgcggcgc
     2401 acatccacat cggcatcgag tggctcgcgt cggtctcgtt cggccacgtc acgcagatcg
     2461 gtcgacgcgt gatcgtgctc ggcggcggca acaccgcgat ggactgctgc cgcagcgcca
     2521 agcgcctcgg cggcgacgac gtgaaggtga tcgtgcgctc gcccttcgac gagatgcgcg
     2581 cctcgccgtg ggagaaggaa gacgcggcgc acgagggcat cgcgatcctc gactgcctgg
     2641 tgccgaccgc gttcgtgcac gagggcggca agctgaccgg catgcgcttc gagaaggtgc
     2701 gcgcgctgcg cgacgccgcc ggccgccgcc agctcgtgcc gaccggcgag cccgagcatg
     2761 tttacgcgtg cgacgaggtg ctggtcgcgg tcggccagga gaatgcgttc ccgtggatcg
     2821 agcgcgacgc cggcatcgcg ttcgacgcgc acggcatgcc ggtgctcgac gctgtcaccc
     2881 tgcagtcgag ccggccgaac gtgttcttcg gcggcgacgc ggcgctcggc ccgaagaaca
     2941 tcatctgggc agtcgcccag ggccacgcgg cggcgatctc gatcgacaag ttcctgcacg
     3001 gcgaaaacgt cgcagtgcgg ccggcgccgg gcgtcacgct ggtgtcgcag aagatgggca
     3061 tccacgagtg gagctacgac aacgcggtca cgagcgacac gcgtcacgcg gtgccgtggc
     3121 gcgaggtggg caaggcgctg cgcgacatca aggtcgaggt cgaactcggc ttcgacgccg
     3181 aactcgcgtg gaacgaagcg cagcgctgcc tgaactgcga cgtgcagacc gtgttcaaca
     3241 cgcgccagtg catcgagtgc gacgcctgcg tcgacatctg cccgaccgac tgcatcagct
     3301 tcacggcgaa cggcattgag gccgacctgc gcacgcgcct gcgcgcgccg gcgcgcaaca
     3361 tcgaccaggc gctgtacgtc tcggacgagc tcaagaccgg ccgcgtgatg gtgaaggacg
     3421 aagacgtgtg cctgcactgc ggcctgtgcg ccgagcgctg cccgaccggc gcgtgggaca
     3481 tgcagaaatt tctcatcgac atgacgcacg cgggccctgc gtgccgtaac ccgaaacgga
     3541 aagccgcatg aagcaggcag tcaacgattt cgtcgtcaag ttcgccaac
//
LOCUS       DASRDG010000283         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_614498, whole genome shotgun sequence.
ACCESSION   DASRDG010000283 DASRDG010000000
VERSION     DASRDG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614498"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFA35_04695"
     CDS             <1..409
                     /locus_tag="VFA35_04695"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZT55501.1"
                     /translation="PTSPNPFGDLGQMIEQFKLPGVDMGAIVASGRKDMEALLATNKA
                     MLDSMQELGKRQAEVFAQAMKSAQGSIQTFAAGGGLPDPAKQAELARTAYEKAVVEMN
                     ELGQMARKAQADAMAGITERAQQSVEALKKFGK"
     gene            508..2196
                     /gene="phaC"
                     /locus_tag="VFA35_04700"
     CDS             508..2196
                     /gene="phaC"
                     /locus_tag="VFA35_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HZT55502.1"
                     /translation="MSLPFNMPDEYVQGLMKAGQNLLQTLAKPPGLPGETSTSLGELQ
                     LNYFRQQFALWSRMMGGAGAADEPVVAADRGDRRFHATEWRENPAYSLLKQSYLLNSR
                     LISDVVEASDVDDATKHKLRFFAKQFIDAMSPANFAATNPDVLKLAMETKGESLKAGF
                     ENMLADLRKGGLTITDENAFEVGKNVAVSEGAVVFENELFQLIQYAPLTDKVAARPLV
                     IVPPCINKFYILDLQPENSFVRYATEQGQTVFLVSWRNTDASGGNVTWDDYIEHGAMK
                     ALAVAHEVSGADKVNAVGWCVGGTILSSTLAVLRTRGDESVASLTLLTTLLDFREPGD
                     LGVLIDEQGVQQREQTIGKGGVYSGAELGFVFQTLRANDLIWPNVINNYLKGKSPEAF
                     DLLYWNADATNLPGPMYAYYLRNMYLENNLRVPDKLTMCGTPVDLGRIDMPSFVLATK
                     EDHIVPWRSAYYTTQLVGGKSQFVLGASGHIAGVINPASKNKRSYWSGGKQGDDAEAW
                     LASATETPGSWWNHWIKWLQPHTGKPVAARKRLGNAKHKPIEPAPGRYVKVKAS"
     gene            2209..2997
                     /locus_tag="VFA35_04705"
     CDS             2209..2997
                     /locus_tag="VFA35_04705"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZT55503.1"
                     /translation="MNYDNILTAEHGEGALRTGVITLHRPKQLNALNDALMDDLGAAL
                     KAYDADDGIGCIVVTGSEKAFAAGADIAAMAEFDHMQVYKTGYITRNWETITTIRKPV
                     IAAVAGFALGGGCELAMMCDFVIAADNAKFGQPEIKLGILPGAGGTQRLPRAVGKAKA
                     MDMVLTSRMIDAAEAERAGLVSRVVPADKLMDEALGAAATICGLSLPSVMMAKEAVNR
                     AFEAPLGEGIRFERRLFHSLFGSADQREGMAAFLGKRKPSFQHR"
     gene            3020..>3341
                     /locus_tag="VFA35_04710"
     CDS             3020..>3341
                     /locus_tag="VFA35_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT55504.1"
                     /translation="MSTPEASSGTPHRVVIIGGGAAGLELATTLGNKLGKRGRVQVTL
                     IDKARSHVWKPKLHEIAAGSMDIGVHEVSYSAQAHWHHFRYRIGELIGLDRQRRQVHI
                     APFVD"
BASE COUNT          629 a         1129 c         1125 g          458 t
ORIGIN      
        1 ccccacctcc cccaacccgt tcggcgacct cggccagatg atcgagcagt tcaagctgcc
       61 cggcgtcgac atgggcgcca tcgtcgcgtc cggccgcaag gacatggagg cgctgctggc
      121 cacgaacaag gccatgctcg attcgatgca ggagctgggc aagaggcagg ccgaggtctt
      181 cgcgcaggcc atgaagagcg cgcaggggag catccagacc ttcgccgcgg gcggcggcct
      241 gcctgatccc gccaagcagg cggagctggc gcgcacggcc tacgagaagg cggtcgtcga
      301 gatgaacgaa ctcgggcaga tggcgcgcaa ggcccaggcc gacgcgatgg ccggcatcac
      361 cgagcgcgcg cagcagagcg tcgaggcgct gaagaagttc ggcaaatagg cgcgacgccc
      421 tgcgcgtcgc ggtgacagca cccgcgcaac ggatgcgcgg tgcccggagg gctgccctcc
      481 ggaaattctt cttgatggag gaagcctgtg agcttgccgt tcaacatgcc cgacgagtac
      541 gtccagggcc tcatgaaagc ggggcaaaac ctgctgcaga ccttggccaa gcccccgggc
      601 ttgccgggtg agacgagcac gtcgctgggc gagctccagc tgaactactt ccgccagcag
      661 ttcgcgctgt ggtcccggat gatgggcggc gccggcgccg cggacgagcc ggtcgtcgcc
      721 gcggaccgtg gcgaccgccg cttccacgcc accgagtggc gcgagaaccc ggcgtacagc
      781 ctgctcaagc agagctacct gctcaactcg cggctgatca gcgacgtcgt cgaggcctcg
      841 gacgtggacg acgcgaccaa gcacaagctg cgctttttcg ccaagcagtt catcgacgcg
      901 atgagcccgg cgaacttcgc ggcgaccaac ccggacgtgc tcaagctggc gatggagacc
      961 aagggcgaga gcctgaaggc cggcttcgag aacatgctcg ccgacctgcg caagggcggc
     1021 ctcacgatca ccgacgagaa cgcgttcgag gtcggcaaga acgtcgcggt atccgagggc
     1081 gcggtggtct tcgagaacga gctgttccag ctgatccagt acgcgccgct gaccgacaag
     1141 gtggccgcgc gcccgctggt gatcgtgccg ccgtgcatca acaagttcta catcctcgac
     1201 cttcagcccg agaactcgtt cgtgcgctat gccaccgagc aagggcagac cgtgttcctc
     1261 gtgtcctggc gcaacacgga cgccagcggc ggcaacgtga cctgggacga ctacatcgag
     1321 cacggcgcga tgaaggcgct cgcggtggcg catgaggtca gcggggccga caaggtcaat
     1381 gcggtcggct ggtgcgtcgg cggcacgatc ctgtcgtcca cacttgcggt gctgcgcacg
     1441 cgcggcgacg agtccgtcgc cagcctgacg ctgctgacca cgctgctcga cttccgcgag
     1501 cccggcgacc tgggcgtgct catcgacgag cagggcgtgc agcagcgcga gcagaccatc
     1561 ggcaagggcg gcgtctattc cggcgccgag ctcggcttcg tgttccagac cctgcgcgcc
     1621 aacgacctga tctggcccaa cgtcatcaac aactacctga agggcaagtc gcccgaggcc
     1681 ttcgacctgc tgtactggaa cgccgacgcg accaacctgc ccgggccgat gtacgcgtac
     1741 tacctgcgca acatgtacct ggagaacaac ctgcgcgtgc cggacaagct gacgatgtgc
     1801 ggcacaccgg tcgacctcgg ccgcatcgac atgccgagct tcgtgctggc gaccaaggaa
     1861 gaccacatcg tgccgtggcg ctcggcctac tacacgacgc agctggtcgg cggcaaatcg
     1921 cagttcgtgc tcggcgcgag cgggcacatc gccggcgtca tcaacccggc gtcgaagaac
     1981 aaacgcagct actggagcgg cggcaagcag ggcgacgacg ccgaggcttg gctggccagc
     2041 gcgacggaaa ccccgggcag ctggtggaac cactggatca agtggctgca accgcacacc
     2101 ggcaagccgg tggccgcgcg caagcggctc ggcaacgcga agcacaagcc gatcgaaccg
     2161 gcgccggggc gctacgtgaa ggtcaaagcc agctgaggca gctacccaat gaactacgac
     2221 aacatcctga ccgccgagca tggcgagggt gcgctgcgca ccggcgtgat cacgctgcac
     2281 cgcccgaagc agctcaacgc gctgaacgac gcgctgatgg acgacctggg cgcggcgctg
     2341 aaagcctatg acgccgacga cggcatcggc tgcatcgtcg tcaccggcag cgagaaggca
     2401 ttcgccgccg gcgccgacat cgcggcgatg gccgagttcg accacatgca ggtctacaag
     2461 acgggctaca tcacgcgcaa ctgggaaacc atcacgacga tccgcaagcc ggtgatcgcg
     2521 gccgtcgccg gcttcgcgct cggcggcggc tgcgaactcg cgatgatgtg cgacttcgtc
     2581 atcgccgccg acaacgcgaa attcggccag cccgagatca agctcggcat cctccccggc
     2641 gccggcggca cgcagcgcct gccgcgcgcc gtgggcaagg ccaaggcgat ggacatggtg
     2701 ctgacctcgc gcatgatcga cgcggccgag gccgaacgcg cggggctcgt ctcgcgcgtc
     2761 gtgccggccg acaaactgat ggacgaggcg ctcggcgcgg cggccaccat ctgcggcctg
     2821 agcctgccga gcgtgatgat ggccaaggaa gccgtcaatc gcgcgttcga ggcgccgctc
     2881 ggcgaaggca tccgcttcga gcggcggctg ttccactcgc tgttcggcag cgccgatcag
     2941 cgggagggca tggcggcctt cctcggcaag cgcaagccgt cgtttcaaca ccgctgaacc
     3001 cgcatcgagg acccccatcg tgtcgacacc cgaggcatct tccggcacgc cgcatcgcgt
     3061 cgtgatcatc ggcggcggcg ccgccggtct ggaactggcg accacgctgg gcaacaagct
     3121 cggcaagcgc ggccgcgtgc aggtgacgct gatcgacaag gcgcgcagcc atgtctggaa
     3181 gcccaagctg cacgagatcg ccgcgggcag catggacatc ggcgtgcacg aggtgtcgta
     3241 cagcgcgcag gcacattggc accacttccg ttatcggatc ggcgagctga tcggcctgga
     3301 tcggcaacgc cgtcaggtgc acatcgcacc gttcgtcgac a
//
LOCUS       DASRDG010000284         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_498394, whole genome shotgun sequence.
ACCESSION   DASRDG010000284 DASRDG010000000
VERSION     DASRDG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498394"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFA35_04715"
     CDS             <1..729
                     /locus_tag="VFA35_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZT55505.1"
                     /translation="AWLSGCHAAGAVLASACSGALLLARTGLLNDLDATTHWAYCETL
                     QREHPRTRWHAERGLVVAGEGQRLVMAGSGVAWHVLVLYLIARFAGPEEAMQVARINL
                     MDLNATSPVAYASLTHGAQSSDPVISRCQQWAAMNYQVESPVTQMVALSGLPERTFKR
                     RFTQVTGMSPLEYVHMLRLEEAKQMLEASDEPIEAIAVEVGYQDTSFFGRLFRRKVAL
                     SPAQYRRRFKPLGEQLRAAAPRNP"
     gene            744..1322
                     /locus_tag="VFA35_04720"
     CDS             744..1322
                     /locus_tag="VFA35_04720"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZT55506.1"
                     /translation="MTLRTIVCTLALAATTAAFAALNAGDPAPAFSAPASLAGKPFTY
                     SLKDGLAKGPVVVYFYPSAYTVGCNVQAHEFSTKIDEFAAAGATVIGVSLDSIERLNE
                     FSADPSYCAGKLAVASDGDGKIARAFGLRVSGAKSGAVDTRGKEIDHGFTERTTFVVK
                     RDGTVFAAVGGVSPEANVAQALELVRTLSATR"
     gene            complement(1319..2650)
                     /locus_tag="VFA35_04725"
     CDS             complement(1319..2650)
                     /locus_tag="VFA35_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZT55507.1"
                     /translation="MTPQQPQGEPERLDGSALAINVRHLHALSAVAAAGSVAGAAEAL
                     CRVSSAVTRSIAELERSLGRPLFERRARGMAITSYGELVLARTRHIEREFEAAREHLA
                     ARATGAASTDVHSLFASILNGRRLAVIAGLADRRSMPAVAREFGLSQPAISAALKDLE
                     SRLGLALFERSARGLVATVAGEIVAFHFKRVLAELRHIVPDIAASEGTLQGRVRVGAL
                     PLGRTQVLPLAIASLLARHPNLHVATDESPYDTLAVSLRSGDIDFILGALRSGAAAKD
                     LQQEPLFEDRMALICRAGHPLARARRIDFELLRRATWVLSRRGAPARDLLEQFFLAGR
                     QVPPIPAVETGDLAVLRGLLLESDMVTAISAHQLRYEIRDQTLVVLDFPLDQTRREIG
                     IAQRQGCSPSPGARALMDEIRGLVAGSADFQTHAVARRDCEAAGRSAKGGG"
     gene            2775..>2938
                     /locus_tag="VFA35_04730"
     CDS             2775..>2938
                     /locus_tag="VFA35_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HZT55508.1"
                     /translation="MALFIGGIAASHTPTIGFAFDRDKRDDPVWAPIFANFAPLRTWL
                     EQQRPEVLLFV"
BASE COUNT          445 a         1026 c         1047 g          420 t
ORIGIN      
        1 gcctggctga gcggctgcca cgccgccggc gcggtgctcg cctccgcctg ctcgggcgcc
       61 ctgctacttg cgcgcaccgg gctgctgaac gacctcgacg ccacaacgca ctgggcctac
      121 tgcgagacgc tgcagcgcga gcatccgcgc acccgctggc atgccgagcg cgggctggtc
      181 gtggccggcg aaggccagcg cctcgtgatg gccggcagcg gcgtcgcctg gcacgtgctg
      241 gtgctctacc tgatcgcccg cttcgccggg cccgaagagg cgatgcaggt ggcccgcatc
      301 aacctcatgg acctcaacgc cacgagcccg gtggcctatg cgtcgctgac gcacggcgcg
      361 cagtccagcg atccggtgat ctcgcgctgc cagcaatggg cggcgatgaa ctaccaggtc
      421 gagtcgccgg tgacgcagat ggtggcgttg tcggggctgc ccgagcgcac cttcaagcgc
      481 cggttcacgc aggtcacggg aatgagcccg ctcgaatacg tgcacatgct gcgcctcgag
      541 gaggccaagc agatgctcga ggcgagcgac gagccgatcg aggcgatcgc ggtcgaggtc
      601 ggttaccagg acacgagctt tttcggccgg ctgtttcggc gtaaggtagc cctttcgccc
      661 gcgcagtacc ggcgacgctt caagcccctc ggcgagcaac tgcgcgcggc ggcgccacga
      721 aacccctgac ggaggtcacc agcatgacac tacgcacgat cgtctgcacg ctcgccctgg
      781 ccgcgaccac ggccgctttc gccgcgctga atgcgggcga tccggcccca gcgttcagcg
      841 cccccgcctc gctggcgggc aagcctttca cctactcgct gaaggacggc ctggccaagg
      901 gtccggtggt cgtctacttc tatccgtcgg cctacaccgt gggatgcaat gtccaggcgc
      961 acgagttctc gaccaagatc gacgagttcg cggccgccgg cgcgacggtg atcggcgtct
     1021 cgctcgacag catcgaacgg ctgaacgagt tctcggccga cccctcctat tgcgccggca
     1081 agctcgcggt cgcgtccgac ggcgacggca agatcgcgcg cgccttcggc cttcgggtga
     1141 gcggcgcgaa aagcggtgcc gtcgacaccc gcggcaagga aatcgaccac ggcttcaccg
     1201 agcgcacgac cttcgtcgtc aagcgcgacg ggaccgtctt cgcggcggtg ggtggcgtct
     1261 cgccggaagc caacgtcgcg caggcgctgg agctggtgcg gacgctgtcg gccacgcgct
     1321 agccgccgcc tttcgccgat cggcctgcgg cctcgcagtc gcgtcgggcg accgcgtgcg
     1381 tctggaaatc cgcagagccc gcgaccaggc cgcggatctc gtccatcagg gcgcgtgcgc
     1441 cgggcgatgg cgagcagccc tggcgctggg cgatgccgat ctcccggcgc gtctggtcga
     1501 gcgggaagtc gagcacgacc agcgtctggt cgcggatctc gtagcggagc tggtgcgccg
     1561 agatcgccgt gaccatgtcg ctttcgagga gaaggccgcg cagaaccgcg agatcgccgg
     1621 tctcgaccgc cgggatcggc ggcacctgac ggccggcgag gaagaactgc tccagcaagt
     1681 cgcgcgcggg cgcgccgcga cgcgacagca cccaggtggc acggcgcaga agctcgaagt
     1741 cgatccggcg agcgcgggcc agcggatggc cggcgcggca gatcagtgcc atgcggtcct
     1801 cgaacagcgg ctcctgctgc aggtctttcg cggccgcgcc gctgcgcaac gcaccgagga
     1861 tgaagtcgat gtcgccgctg cgcagcgaga ccgccagcgt gtcgtacggg ctctcgtcgg
     1921 tggcgacgtg caggttcgga tgtcgggcca gcagcgaggc gatggccagc ggcagcacct
     1981 gcgtgcgacc gagcggcagc gcgccgacgc gaaccctgcc ctgcagcgtg ccctcgctgg
     2041 cggcgatatc ggggacgatg tggcgcagct cggccaggac gcgcttgaaa tggaaggcga
     2101 cgatctcgcc ggccacggtg gcgaccagcc cgcgcgccga gcgttcgaac agggcgagcc
     2161 ccaggcggct ttcgagatcc ttcaaggccg cgctgatcgc cggctgactg aggccgaact
     2221 cgcgcgccac cgccggcatg ctgcgccggt cggcgaggcc ggcgatgacg gcgaggcggc
     2281 ggccgttgag gatcgaggcg aacagcgagt ggacgtcggt cgaggccgcg ccggtcgcac
     2341 gcgccgccag gtgctcccgg gccgcctcga actcgcgctc gatgtgccgt gtgcgcgcga
     2401 gcaccagctc gccgtagctg gtgatggcca tgccgcgcgc ccgacgctcg aagagcggcc
     2461 ggccgaggct gcgctcgagc tcggcgatcg agcgcgtcac cgccgacgag acgcggcaga
     2521 gcgcttcggc agcgccggca acgctccccg cggcggccac cgccgacagg gcgtgcaggt
     2581 ggcgcacatt gatggcaagg gccgagccgt cgagtcgttc gggttcgcct tgcggttgct
     2641 gcggagtcac ccttcgaatc tcgtgcggcg tagcgcgggt gtctacgccg ggttaacccg
     2701 aggacataag aaagtctgac ggcagcgaag ggcgaactca ccccgttcgc catgtccggc
     2761 gacgaaactg gtgcatggcc ctcttcatcg gcggcatcgc cgcctcgcac acgccgacca
     2821 tcggcttcgc cttcgatcgc gacaagcggg acgatccggt ctgggcgccg atcttcgcca
     2881 acttcgcgcc gctgcgcacc tggctcgagc agcagcggcc cgaggtgctg ctcttcgt
//
LOCUS       DASRDG010000285         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_968787, whole genome shotgun sequence.
ACCESSION   DASRDG010000285 DASRDG010000000
VERSION     DASRDG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968787"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2316)
                     /gene="dnaE"
                     /locus_tag="VFA35_04735"
     CDS             complement(<1..2316)
                     /gene="dnaE"
                     /locus_tag="VFA35_04735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZT55509.1"
                     /translation="MADAPFVHLRTRSHYSLLSAPVAIDDLVAAAAADGQTALALTDN
                     GNLFGAVEFYKACQQKGVKPVLGMTTFVAGRTMREPFGPDNPTHDLTLLAADARGFAN
                     LRQLASKAWLQGFSYRPRVDLDALHSHREGLIALSGSISGQVAQAIQQDDVEAAKAAA
                     GRLRELFGADNFFLEVAATGAEVQRKVTAGKQRLSAALGIPCVATNDVHYLRPDDWLA
                     HDIMLCIRNGKTVADQDRFRMSSRELYLKSRAQMQEAFGDWQAPVQQSAAIAARCNVA
                     MGFGVYHVPVFQPETGETPSAYFARRCREGAVMRYGSLRPEVEQRLEYEIQVVEKSGF
                     VSYFLIVQDFILKAKSLGIPVGPGRGSAAGSLVAYALQITDVDPLRYALLFERFLNPG
                     RVSMPDIDIDFCGDRRGEVIDYVRRKYGDDCVCQIITFGTMASRGVLRDVGRVLDFDL
                     GEVDQLCKKVPQGPGASLEAALQADPELKAIYQGSPQHKRLFDLALQLEGLARHSSVH
                     AAGVVIADRPLQDYVPLCKNGEDVITQWQMTDLEDVGLLKMDFLGLKTLTILAEGARL
                     VREVHGTTLDLSTLPLDDRDTYALMARGETQGVFQLESGGMRELLGRLKPDTFEDVIA
                     VLALYRPGPLGSGMVETFVRRKHGEEKTVYPHADLEPVLKPTYGVIVYQEQVMQIANV
                     IGGFSMTDADNLRKAMGKKKPEVMAKYKDQFVDGAKGRGYGEKFAKDLFETMEFFAGY
                     GFNKSHSTAYALVTYQTAWLKAHYPLAFLASN"
BASE COUNT          371 a          879 c          856 g          401 t
ORIGIN      
        1 gttcgaggcc aggaacgcga gcgggtagtg cgccttgagc caggcggtct ggtaggtgac
       61 cagcgcgtac gccgtcgagt gcgacttgtt gaagccgtag ccggcgaaga actccatcgt
      121 ctcgaacagg tccttggcga acttctcgcc gtagccgcgt cccttggcgc cgtcgacgaa
      181 ctggtccttg tacttcgcca tcacctcggg cttcttcttg cccatcgcct tgcgcaggtt
      241 gtcggcgtcg gtcatcgaga acccgccgat cacgttggcg atctgcatca cctgttcctg
      301 gtagacgatg accccgtagg tcggcttcag caccggctcg aggtcggcgt gcgggtagac
      361 ggtcttctcc tcgccgtgct tgcgccgcac gaacgtctcc accatgcccg agccgagcgg
      421 gccgggccgg tagagcgcca gcacggcgat cacgtcctcg aacgtgtcgg gcttgagccg
      481 gccgagcagc tcgcgcatgc cgcccgattc gagctggaac acgccttgcg tctcgccgcg
      541 cgccatcagc gcgtaggtgt cgcggtcgtc gagcggcagc gtcgacaggt cgagcgtggt
      601 gccgtgcacc tcgcgcacga gacgcgcgcc ctcggcgagg atcgtcagcg tcttgaggcc
      661 gaggaagtcc atcttgagca ggccgacgtc ctcgaggtcg gtcatctgcc actgcgtgat
      721 cacgtcctcg ccgttcttgc acagcggcac gtagtcctgc agcggccggt ccgcgatcac
      781 gacgcccgcc gcgtgcaccg acgagtggcg cgcgagacct tcgagctgca gcgccagatc
      841 gaacaggcgc ttgtgctgcg gcgagccctg gtagatggcc ttcagctcgg ggtccgcctg
      901 cagcgccgcc tcgagcgacg cgccgggacc ctgcggcacc ttcttgcaca gctggtcgac
      961 ctcgccgagg tcgaagtcga gcacgcggcc gacgtcgcgc agcacgccgc ggctcgccat
     1021 ggtgccgaac gtgatgatct ggcagacgca gtcgtcgccg tacttcctgc gcacgtagtc
     1081 gatcacctcg ccgcgccgat cgccgcagaa gtcgatgtcg atgtcgggca tcgacacgcg
     1141 gcccgggttc aggaaacgct cgaacagcag cgcgtagcgc aacgggtcga cgtcggtgat
     1201 ctgcagcgcg tacgcgacca gcgagccggc cgcggagccg cggccgggac cgaccgggat
     1261 cccgagcgac ttcgccttca ggatgaagtc ctgcacgatc aggaagtagc tgacgaagcc
     1321 ggacttctcg acgacctgga tctcgtactc gagccgctgc tcgacctcgg gccgcagcga
     1381 gccatagcgc atcacggcgc cctcgcggca gcggcgcgcg aagtacgcgc tcggcgtctc
     1441 gccggtctcc ggctggaaca ccggcacgtg gtagacgccg aaccccatcg cgacgttgca
     1501 gcgcgccgcg atcgccgccg actgctgcac gggcgcctgc cagtcgccga acgcctcctg
     1561 catctgcgcc cgcgacttca ggtacagctc gcgcgagctc atgcggaacc ggtcctggtc
     1621 ggcgacggtc ttgccgttgc ggatgcacag catgatgtcg tgcgccagcc agtcgtcggg
     1681 ccgcaggtag tgcacgtcgt tggtcgcgac gcacgggatg ccgagcgctg ccgacagccg
     1741 ctgcttgccg gccgtgacct tgcgctgcac ctcggcgccg gtcgccgcga cctcgaggaa
     1801 gaagttgtcg gcgccgaaca gctcgcgcag ccgtcccgcc gcggccttcg cggcctcgac
     1861 gtcgtcctgc tggatcgcct gcgcgacctg accgctgatc gagccgctca acgcgatcag
     1921 gccttcgcgg tggctgtgca gcgcgtcgag gtcgacgcgc ggccggtagc tgaagccctg
     1981 cagccacgcc ttcgacgcca gctgccgcag gttggcgaag ccgcgcgcgt cggcggcgag
     2041 cagcgtgagg tcgtgcgtcg ggttgtccgg tccgaacggc tcccgcatcg tgcggccggc
     2101 gacgaacgtc gtcatcccga gcacgggctt cacgcccttc tgctggcacg ccttgtagaa
     2161 ctcgacggcg ccgaacaggt tgccgttgtc ggtcagcgcg agcgccgtct gcccgtccgc
     2221 ggccgcagcg gcgaccaggt cgtcgatcgc gaccggcgcg ctgagcaggc tgtagtgcga
     2281 gcgcgtgcgc aggtggacga aaggcgcgtc ggccatcgcg cacagactac gggccgcttc
     2341 cgcggccaga aagagcgcgc gtcagctgcc ggcggccttg gcgcgcaccg agcccttaag
     2401 cggcagcacg gacgccttct tgcccgcctc gacgaagcgc gacagcacca ggccgatctg
     2461 ctcgtcggtg tgcgtcgcca tgaaggacgt gcggatcagg tccatgc
//
LOCUS       DASRDG010000286         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_848194, whole genome shotgun sequence.
ACCESSION   DASRDG010000286 DASRDG010000000
VERSION     DASRDG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848194"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="VFA35_04740"
     CDS             complement(<1..1756)
                     /locus_tag="VFA35_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZT55510.1"
                     /translation="MSSLENHLWTHAKALRRLASCLVGESAADDLVQETALEALQMPE
                     ARPRALQAWLRAVLRNLAGKHRRGRRCRERHERQAPPPAASMPPDRLAEQREIVRRLD
                     GALLELPEPYQGTLLRRFFGGLSPSEIAAQTRVPLATVKSRLQRGLALLRERLQRDGA
                     DSGWWQALAVFGGRTGGIGTAAAATTTTGVLLMGTGAKLAIGATAAVLAVATSLWMAS
                     GAPAPQQLPLSADARLAVAPATAGAGAPVAAAGTAASVTDAPDAQRERLAAAAPANGE
                     DSADPPLRIRVVDAEGRPVGDAPVGLRDGVQATAAVVWRGRTAADGIADSKHPRALLR
                     DAGGPVFATLLVPIRPLPAAQLDPAALPREPVQLVLPATGGVQVWIDDLTPAEAEQVT
                     VTLGAADAQATNEFMPVASQHPAGGRADFPFVGLGLSLLVRAQRSGQNDDTHVVVTGP
                     AAPGEIVPVRLHVQPLPERIVLVVHLLGADAQAFRRQKIDVALAARGNGGSTTGTFDI
                     DTDGDGVLRIAVEPTWRTMPDRSLRLRGRCGEPPEGCEAMVPLPPAFAPGENDLGTVH
                     LVPPPLVCAGIVVNRDGAA"
BASE COUNT          259 a          783 c          754 g          242 t
ORIGIN      
        1 ccgcggcgcc atcgcggttc acgacgatgc ccgcgcagac gagcggcggc ggcaccaggt
       61 gcacggtgcc gagatcgttc tcgccgggcg cgaacgccgg cggcagcggc accatggcct
      121 cgcacccctc gggcggctcg ccgcaccggc cgcgcaggcg cagcgaccgg tcgggcatcg
      181 tccgccaggt gggctcgacg gcgatgcgga gcacgccgtc gccgtcggtg tcgatgtcga
      241 acgtgcccgt ggtcgagccg ccgttgccgc gcgctgcgag cgcgacgtcg atcttctgcc
      301 ggcggaacgc ctgcgcgtcg gcgccgagca ggtgcacgac gagcacgatc cgttcgggca
      361 gcggctgcac gtgcagccgc accggcacga tctcgccggg ggcggcaggt ccggtgacga
      421 cgacgtgcgt gtcgtcgttc tggccgctgc gctgcgcgcg cacgagcagc gacaacccga
      481 gccccacgaa cgggaagtcc gcgcggccgc cggccggatg ctgcgacgcg accggcatga
      541 actcgttcgt ggcctgcgcg tccgccgcgc cgagcgtgac ggtcacctgc tccgcttccg
      601 caggcgtgag atcgtcgatc cagacctgca cgccgccggt cgccgggagc accagctgca
      661 cgggttccct gggcaatgcc gccgggtcga gctgcgccgc cggcagcggc cggatcggca
      721 cgagcagcgt cgcgaacacg ggaccgccgg cgtcgcgcag cagcgcacgc ggatgcttgc
      781 tgtccgcgat gccgtcggcg gcagtgcgcc cgcgccacac gaccgccgcg gtcgcctgca
      841 cgccgtcgcg gagaccgacc ggcgcgtcgc cgaccggccg gccttcggca tcgacgacgc
      901 ggatccgcag cggcgggtcg gcggaatcct cgccgttcgc cggcgcggcg gcagcgagcc
      961 gctcgcgctg cgcgtccggt gcgtcggtca cgctggccgc cgtccccgcg gcggcgaccg
     1021 gcgcgcccgc gcctgcggtc gccggcgcga ccgcgagccg tgcgtcggca gacaggggca
     1081 gttgctgcgg cgcaggcgcg ccggacgcca tccacagcga ggtcgccacg gccaacactg
     1141 ccgccgtggc cccgatcgcg agcttcgctc ccgttcccat cagcaagact cctgtcgtcg
     1201 tagtggccgc cgcagcggtc ccgatgccgc cggtccgccc gccgaacacg gccaatgcct
     1261 gccaccagcc gctgtcggcg ccgtcccgct gcagccgttc gcgcagcagc gcgagcccgc
     1321 gctgcaggcg gctcttcacc gtcgccagcg gcacgcgcgt ctgcgcggcg atctcgctcg
     1381 gcgacagccc gccgaagaag cgccgcagca acgtgccctg gtacggctcg ggcaactcga
     1441 gcagtgctcc gtcgaggcgg cgcacgatct cgcgctgctc ggccagccgg tcgggcggca
     1501 tgctcgcggc cggcggcggt gcctgccgtt cgtgccgttc ccggcaccgg cgcccgcgcc
     1561 gatgcttgcc cgcgaggttg cggagcacgg cgcggagcca cgcctgcaac gcccgcggcc
     1621 gcgcctcggg catctgcagc gcctcgagcg cggtctcctg caccaggtcg tcggccgcac
     1681 tctcgccgac caggcagctg gcgagccggc gaagcgcctt ggcgtgcgtc cagaggtggt
     1741 tctcgaggga agacatggcg gcgcgcacct gcgcgcgtgc atcctgtgta ccccggcgaa
     1801 ggatcgggga tgcagacgag aggggactcc gcgccgcgag gctccgccgg gcccgacggc
     1861 agggcgtcag cggcgttgcg gctcgaacgc tccgtccgca tcgcgccgga accgcgcgcc
     1921 gcagtgcagg caccagcggt tcggatggat gcggaacacg acccagctca cgggcggcag
     1981 gcaccacgcg acgaggaacc agaatgcact cggccagtcg cgcgcgcagt tcgtcgac
//
LOCUS       DASRDG010000287         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1195695, whole genome shotgun sequence.
ACCESSION   DASRDG010000287 DASRDG010000000
VERSION     DASRDG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195695"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFA35_04745"
     CDS             complement(<1..295)
                     /locus_tag="VFA35_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT55511.1"
                     /translation="MISLQQLTKGFGGPALAVDRLSLEVPAGQILGLLGPNGAGKTTT
                     LKMVTGMLAPDFGTALICGVDIVRQPLEAKRLLGFVPDSAAVYEALSGLEFLLM"
     gene            387..1235
                     /locus_tag="VFA35_04750"
     CDS             387..1235
                     /locus_tag="VFA35_04750"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZT55512.1"
                     /translation="MTTIALLGTGLLGSGMVENLLRKGNTVRVWNRSATKVAPLVQQG
                     AVACKDPADAVRGCERAHLVLAEDAAVDAVITALRPGLDKNVPLIDHSTNLPAKVAAR
                     FTALRAAGVRYVPAPVFMSPQNAREASGLMLLSAPKADADALSPALSAMTGKLWHVGE
                     RPDLGALLKLAGNGMLIAMVGTIGDLLAMAKSNGVPAEQVLGLFDVFKAGASLPFFGQ
                     RVLKAGEGPASFELQMARKDVRLMLETAGKAPTIVLPALAAAMDKALARGRGSQDFAV
                     FARAEG"
     gene            complement(1256..>2013)
                     /locus_tag="VFA35_04755"
     CDS             complement(1256..>2013)
                     /locus_tag="VFA35_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZT55513.1"
                     /translation="GDPLKLDLKIGSQYYRVVGVLPDYATANPDRSVLGLDDRALEVY
                     VPFATVVDRLGLTKGNYESGNYEISRVELHQIVCTVGEEADVPVAARDIHAILERFHE
                     RKDYEVTVPIELLASRARTQRTFDIVLPVIAGISLLVGGIGILNIMLASVTERTREIG
                     IRRAIGATELDITVQFLIETVTLAVVGGLLGIVLGVAGTRLIERATDWQPVITPGAVL
                     LAMGISSATGVVFGLYPARRAARMDPVRALRHE"
BASE COUNT          299 a          708 c          734 g          272 t
ORIGIN      
        1 ccatgagcag gaactcgagc ccgctgagcg cttcgtagac ggcggccgag tcgggcacga
       61 agccgagcag gcgcttggcc tcgagcggct gccgcacgat gtcgacgccg cagatcagcg
      121 cggtgccgaa gtcgggcgcg agcatgccgg tgaccatctt cagcgtcgtc gtcttgccgg
      181 cgccgttcgg cccgagcagg ccgaggatct ggcccgctgg cacctccagc gacagccggt
      241 ccaccgccag cgccgggccg ccgaacccct tcgtcagctg ctgcagcgag atcacggggc
      301 ggcatcatgc gcgcgccgcc gccgccgaca agggccgcgg cgtctcaccg gttgcggggc
      361 agcgaccgcg gtgccaacct gtgcgcatga ctaccatcgc actcctgggc acaggtctcc
      421 tcggcagcgg catggtcgag aacctgttgc gcaagggcaa caccgtccgc gtctggaacc
      481 gttcggcgac gaaggtggcg ccgctggtcc agcagggcgc cgtcgcgtgc aaggatcccg
      541 ccgacgccgt gcgcggctgc gagcgcgcgc acctcgtgct cgccgaggac gccgcggtcg
      601 acgccgtgat caccgcgctg cgccccggcc tcgacaagaa cgtgccgctg atcgaccact
      661 cgaccaacct gccggcgaag gtcgccgcgc gcttcaccgc gctgcgcgcc gccggcgtgc
      721 gctacgtgcc ggcgccggtg ttcatgtcgc cgcagaacgc gcgcgaggcg agcgggctga
      781 tgctgctgtc ggcgccgaag gccgacgccg acgcgctctc gcccgcgctg tcggcgatga
      841 cgggcaagct gtggcacgtc ggcgagcggc ccgacctcgg cgcactgctc aagctcgcgg
      901 gcaacggcat gctgatcgcg atggtcggca cgatcggcga tctgctggcg atggccaaga
      961 gcaacggcgt gcccgccgaa caggtgctcg gcctgttcga cgtgttcaag gccggcgcgt
     1021 cgctgccgtt cttcggccag cgcgtgctga aggccggcga ggggccggcg agcttcgagc
     1081 tgcagatggc gcgcaaggac gtgcggctga tgctcgagac cgccggcaag gcgccgacga
     1141 tcgtgctgcc cgcgctcgcc gcggcgatgg acaaggcgct cgcgcgcggc aggggcagcc
     1201 aggacttcgc ggtgttcgcg cgcgccgagg gctgaacgcg gcccgagcgg gcggttcact
     1261 cgtgccgcag cgcccgcacc ggatccatgc gcgccgcgcg ccgggccgga tagaggccga
     1321 acacgacacc ggtggcggag gagatcccca tcgcaagcag cacggcgccg ggcgtgatca
     1381 ccggctgcca gtcggtcgcg cgctcgatca gccgggtgcc ggccaccccc agcacgatgc
     1441 cgagcaggcc gccgaccacc gcgagcgtca cggtctcgat caggaactgc acggtgatgt
     1501 cgagctcggt cgcgccgatc gcgcggcgga tgccgatctc gcgcgtccgt tcggtgacgc
     1561 tcgcgagcat gatgttgagg atgccgatgc cgccgacgag cagcgagatg ccggcaatga
     1621 ccggcagcac gatgtcgaac gtgcgctgcg tgcgcgcacg cgacgccagc agctcgatcg
     1681 gcaccgtgac ctcgtagtcc ttgcgctcgt ggaagcgctc gaggatcgcg tggatgtcgc
     1741 gcgcggccac cggaacgtcg gcctcctcgc cgacggtgca gacgatctgg tgcagctcga
     1801 cgcgcgagat ttcgtagtta ccggactcgt agttgccctt ggtcagcccg agccggtcga
     1861 ccacggtcgc gaacggcacg tagacctcga gcgcgcggtc gtcgaggccg agcaccgagc
     1921 ggtcggggtt ggccgtcgcg tagtccggca gcacgccgac gacgcggtag tactgcgacc
     1981 cgatcttgag gtcgagcttg agcggatcgc cga
//
LOCUS       DASRDG010000288         3992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1076510, whole genome shotgun sequence.
ACCESSION   DASRDG010000288 DASRDG010000000
VERSION     DASRDG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076510"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /gene="pcnB"
                     /locus_tag="VFA35_04760"
     CDS             complement(<1..1282)
                     /gene="pcnB"
                     /locus_tag="VFA35_04760"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HZT55514.1"
                     /translation="MIKKFIDKLLGKAPKTGAAAQDGGAAPAGRRVEVPKSEHHIDPS
                     LVDERAVKVVSTLADAGYEAYIVGGAVRDLLVGMRPKDFDVATNATPEQVKALFRRAF
                     IIGRRFRIVHVVFGRGRQDRGQSEVIEVSTFRARVDNVAAEQVSGNESTSKSELAGKS
                     HVVDAEGRVLRDNVWGPQIDDAARRDFTINAMYYDPVKEIVVDYHGGIKDVQRKLLRM
                     IGDPATRYREDPVRIIRVVRFAAKLGFEIEAKTRAPIKEMAALLDNVPPSRTFDEMIK
                     LLQTGHALASIQELRKQGLHRGVFPVLDVALDESQRVDGREKFVRLALADTDRRVQEG
                     KPVAPSFMLACMLWHDVQGGWNKLKAQGEPAFPALQQAVDTVFDARIGDISGRGKLAA
                     DMREIWLMQTRFERRQGNSVATLVEQPRFRAGLDF"
     gene            complement(1306..1986)
                     /locus_tag="VFA35_04765"
     CDS             complement(1306..1986)
                     /locus_tag="VFA35_04765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZT55515.1"
                     /translation="MSNAADLCLFDLDDTLLPMDSDHAWGEFMVRSGWVDAAEFARRN
                     DAFYAQYKAGRLDIHEYIAFATEPLRVRTTLEADAAHRRFMAEVIAPNLRPAALELVR
                     EHQARGDLVALVTATNDFITASIAQAFGIADLIAVRLARGAGGTITGCIEGTPSYREG
                     KVTRVQEWLAERGKTWADFGRISVYSDSVNDLPLLERATDPVATNPTPALRQIARSRG
                     WRVLKLFD"
     gene            complement(1983..2654)
                     /gene="hda"
                     /locus_tag="VFA35_04770"
     CDS             complement(1983..2654)
                     /gene="hda"
                     /locus_tag="VFA35_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HZT55516.1"
                     /translation="MRQIPLAIGPEAPHTFDTFVAGANAPAIAHLQALGAQAAPVYLW
                     GPSGSGKTHLLAALVHRLAAQGLRSGWFSAADAAPWPFDDAWSLVVIDAADALDAAAQ
                     HAAFSVFVEATTRGVPLVAAGRVPPIDLPLREDLRTRLGWGHVFAVQPLGEAEARATL
                     RRVADRRGIFLSDDVMDYLLTRFARDLKHLIAQLDRLDEFSLSTQRRITVPLLKQMLA
                     EEGAA"
     gene            complement(2651..3712)
                     /locus_tag="VFA35_04775"
     CDS             complement(2651..3712)
                     /locus_tag="VFA35_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZT55517.1"
                     /translation="MTFTTAQRQTLTWIVIGAAIVVVVWLLGPVLTPFIVAAVLAYVL
                     HPLVERLARRRVPRALAVTVVEVAAIVIVLAVLLLIVPILSKQLPLLREQIPLLADRA
                     NTQLSPWLARFGVDVALDTASIKAFVLKYLDANLEDWLATALSSARIGGSILIALVGN
                     LILVPMVLFYLLLDWNELVQRSAALIPPRLRERTHNFMDECDATLGQYLRGQLLVMLS
                     LAVYYSVGLALFGFDLAVPVGVFTGLAVFIPYVGFGLGLTLALISGVLQFSGWHGVLG
                     VAVVYGIGQLLEGFVLVPRLVGERIGMSALTVIFALLAFGHLFGFVGVLIALPVSAVL
                     VVAARRLRAAYVESELFHG"
     gene            complement(3792..>3992)
                     /locus_tag="VFA35_04780"
     CDS             complement(3792..>3992)
                     /locus_tag="VFA35_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT55518.1"
                     /translation="RLVVLLPQAWEERRDRGLLYADLGLDAAAARDLSGYLEHAPDAS
                     DRALVADRLAALGGDGGRLRLH"
BASE COUNT          631 a         1391 c         1405 g          565 t
ORIGIN      
        1 ggaagtcgag cccggcacgg aagcgcggct gctcgaccag cgtggccacg ctgttgccct
       61 ggcggcgctc gaagcgcgtc tgcatcagcc agatctcgcg catgtcggcg gccagcttgc
      121 cgcggcccga gatgtcgccg atgcgcgcgt cgaacaccgt gtcgaccgcc tgctgcagcg
      181 ccgggaacgc cggctcgccc tgggccttga gcttgttcca gccgccctgc acgtcgtgcc
      241 acagcatgca ggcgagcatg aagctcggtg caaccggctt gccttcctgg acgcggcggt
      301 cggtgtcggc gagcgcgagc cgcacgaact tctcgcggcc atcgacacgc tgcgactcgt
      361 cgagcgccac atcgagcacc gggaacacgc cgcggtgcag gccctgcttg cgcagctcct
      421 ggatgctcgc cagcgcatgg ccggtctgca gcagcttgat catctcgtcg aaggtgcgcg
      481 atggcggcac gttgtcgagc agcgcggcca tctccttgat cggcgcgcgc gtcttggcct
      541 cgatctcgaa gccgagcttg gccgcgaaac gcacgacgcg gatgattcgc accgggtctt
      601 cgcggtagcg cgtggccggg tcgccgatca tgcgcagcag cttgcgttga acgtccttga
      661 tgccgccgtg gtagtcgacg acgatctcct tgaccgggtc gtagtacatc gcgttgatcg
      721 tgaagtcgcg gcgcgcggca tcgtcgatct gcgggcccca gacgttgtcg cgcagcaccc
      781 ggccttcggc gtcgacgaca tgactcttgc cggcgagctc gctcttcgag gtcgattcgt
      841 tgccgctgac ctgctcggca gcgacgttgt cgacccgggc gcggaaggtc gagacctcga
      901 tcacctcgct ctggccgcga tcctgccggc cgcgcccgaa cacgacatgc acgatgcgaa
      961 agcgccggcc gatgatgaag gcgcggcgaa acagcgcctt gacctgctcg ggtgtggcgt
     1021 tggtcgcgac gtcgaagtcc ttcggccgca ttcccaccag caggtcgcgc accgcgccgc
     1081 cgacgatgta ggcctcgtag ccggcgtcgg ccagcgtgct gacgaccttc accgcgcgct
     1141 cgtcgaccag cgacgggtcg atgtggtgct cgctcttcgg cacctcgacg cgccggcccg
     1201 cgggcgcggc gccgccgtct tgcgcggcgg ccccggtctt gggcgccttg ccgagcagct
     1261 tgtcgatgaa cttcttgatc atgggcggtg tggtttcgtg ctcgatcagt cgaacagttt
     1321 caggacgcgc cagccgcgcg agcgcgcaat ctgccgcagc gccggcgtcg ggttggtcgc
     1381 aaccgggtcg gtggcgcgct cgagcagcgg caggtcgttg accgagtcgc tgtagacgct
     1441 gatgcgcccg aagtcggccc aggtcttgcc gcgctcagcc agccactcct gcacccgcgt
     1501 caccttgcct tcgcggtagc tgggtgtgcc ttcgatgcaa ccggtgatgg tacccccggc
     1561 gccgcgggcc agccgcacgg cgatcagatc ggcgatgccg aaggcctgcg cgatcgacgc
     1621 ggtgatgaaa tcgttggtcg cggtgacgag cgccaccagg tcgccgcgcg cctggtgctc
     1681 gcgcaccagt tcgagcgccg ccgggcgcag gttcggggcg atcacctcgg ccatgaaccg
     1741 gcggtgcgcg gcgtcggcct cgagcgtcgt gcgcacgcgc agcggctcgg tcgcgaacgc
     1801 gatgtactcg tggatgtcga gccggccggc cttgtactgg gcgtagaagg cgtcgttgcg
     1861 gcgcgcgaac tcggccgcgt cgacccagcc cgagcggacc atgaactcgc cccaggcgtg
     1921 gtccgagtcc atcggcagca aggtgtcgtc gagatcgaac aaacagaggt cggcagcgtt
     1981 gctcatgccg caccttcttc ggccagcatc tgtttcagca gcggcacggt gatgcgccgc
     2041 tgggtcgaga gcgaaaactc gtcgagccgg tcgagttgcg cgatcaggtg cttcagatcg
     2101 cgcgcgaaac gggtcagcag gtagtccatc acgtcgtcgg agagaaagat gccgcgccga
     2161 tcggcgacgc ggcgcagcgt ggcgcgcgcc tcggcctcgc cgagcggctg caccgcgaac
     2221 acgtggcccc agccgaggcg cgtgcgcagg tcttcgcgca gcggcaggtc gatcggcggt
     2281 acgcggccgg cggccaccag cggcacaccg cgcgtcgtcg cttcgacgaa gaccgagaac
     2341 gcggcgtgct gcgcagcggc atcgagcgcg tcggcagcat cgatcacgac cagcgaccag
     2401 gcgtcgtcga acggccaggg cgccgcgtcg gccgcgctga accagccgct gcgcagccct
     2461 tgcgcggcga ggcgatgcac cagcgctgcg agcaggtggg tcttgccgct gccgctcggg
     2521 ccccacaggt agaccggcgc ggcctgcgcg cccagggcct gcaggtgcgc gatcgccggc
     2581 gcattcgcgc ccgccacgaa ggtgtcgaat gtgtgcggcg cctcggggcc gatcgccagc
     2641 gggatctgcc tcatccgtgg aacagctcgc tttccacata ggccgcgcgc agccgccgcg
     2701 ccgcgaccac cagcaccgcg ctgaccggca gcgcgatcag cacgccgacg aagccgaaca
     2761 ggtggccgaa cgccagcagc gcgaagatca cggtcagcgc actcatgccg atgcgctcgc
     2821 cgacgagcct gggcacgagc acgaagccct cgagcagctg gccgatgccg tacaccacgg
     2881 ccacgccgag cacgccgtgc cagccgctga actgcagcac gccggagatc agcgccagcg
     2941 tcagcccgag gccgaagccg acataaggga tgaacaccgc cagcccggtg aacacgccca
     3001 ccggcaccgc caggtcgaag ccgaacagcg ccagcccgac gctgtagtag accgcgagcg
     3061 acagcatcac cagcagctga ccgcgcaggt actggccgag cgtggcgtcg cactcgtcca
     3121 tgaagttgtg ggttcgctcg cgcagccgcg gcgggatcag cgcggcgctg cgctgcacca
     3181 gctcgttcca atcgagcagc aggtagaaca gcaccatcgg caccaggatc aggttgccga
     3241 cgagcgcgat caggatgctg ccgccgatgc gcgccgaact caacgcggtg gcgagccagt
     3301 cttcgaggtt cgcgtcgagg tacttcagca cgaaggcctt gatgctggcg gtgtcgagcg
     3361 ccacgtcgac gccgaagcgc gcgagccagg gcgagagctg ggtgttcgca cgatccgcca
     3421 gcagcgggat ctgctcgcgc agcagcggca gctgcttcga caagatcggc acgatcagca
     3481 gcagcaccgc gagcacgatc acgatcgccg cgacctcgac gaccgtgacc gcgagcgcac
     3541 gcggcacgcg ccggcgcgca agccgctcga ccagcggatg cagcacgtag gccagcaccg
     3601 cggcgacgat gaacggcgtc agcaccgggc cgagcagcca caccaccacg acgatggcgg
     3661 cgccgatgac gatccaggtg agggtttggc gctgcgcggt cgtgaaggtc atggtgatcg
     3721 gtggcgcgcg ggcgcccacc atgtcaaggg tggcggaatt ctacgggtcg gcgcgcatgc
     3781 gccgggccgg ctcaatgcag gcgcaagcgc ccgccgtcgc cgccgagcgc ggccagccgg
     3841 tcggcgacca gcgcgcgatc actcgcgtcg ggcgcgtgct cgaggtagcc cgacaggtcg
     3901 cgcgccgccg cggcgtcgag gccgaggtcg gcatagagca ggccgcggtc acgccgctct
     3961 tcccaggcct ggggcagcag cacgaccagg cg
//
LOCUS       DASRDG010000289         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1195904, whole genome shotgun sequence.
ACCESSION   DASRDG010000289 DASRDG010000000
VERSION     DASRDG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195904"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1870
                     /locus_tag="VFA35_04785"
     CDS             <1..1870
                     /locus_tag="VFA35_04785"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HZT55519.1"
                     /translation="EGTVEFAPNYDGSTEEPRLLPARLPFVLLNGASGIAVGLATEIP
                     SHNLREVAAAAIALIRNEMLSDDEFATLLPGPDYPGGGQIISSAEEIRAAYASGRGSL
                     KVRAKWKIEELARGQWQLVVTELPPGASAQKVLEEIEELTNPKVRAGKKTVSTEQAQL
                     KTTVLAVLDAVRDESSKDAPVRIVFEPKSRTTAQPELITTLLAHTSLESSAPINLTMI
                     GADGRPTQKSLREILAEWVAFRAVTVQRRTRHRLDKVLARIHVLEGRQLVLLNIDEVI
                     RIIRESDEPKAALIARFDLSEIQADDILDIRLRQLARLEAIKIEQELKELRVEQGKLE
                     EILSSPSALKRTMIKEIEADAKAHGDDRRTLIQAEKKAVASVLVIDEPVTVVVSLKGW
                     VRGLRGHEVDTAALAFKAGDGLYGTFACRSVDVLLVFGSNGRVYSTPVGQLPGGRGDG
                     QPITSLIDLESGTQPAHYHAGHAQQTLLLAGTGGFGLLARVGDLVARQRGGKSFLSLE
                     VDEKPLPPSLADQQTQVACLSLAGRLLVFALDELKLQSNGGRGLTLIDLDAKDALVSV
                     AAVDQALQIVGTGRGGKPKDEVLKNSALAEYVGKRARKGKKQDVMVKAMRVLRAVR"
     gene            complement(1896..2516)
                     /locus_tag="VFA35_04790"
     CDS             complement(1896..2516)
                     /locus_tag="VFA35_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55520.1"
                     /translation="MRIGSAWPAGAALALLATCASPLLAQTMYRCGNGTGASYLSDKP
                     CSSTGGGVASGNTRLGAYGPVQAPTRGAPTYYPSMAKAPEHLAYMGTECAQLSEGIRT
                     GPARGLAGSTMSELRENYARRCSEDEQQARQRVGQDRSDERAQRKATDQAQRDARNRA
                     VLNREQCYEMLRILHGKRQRAAGMNAGEKADLELFEANYKNRCVAG"
     gene            complement(2581..3054)
                     /locus_tag="VFA35_04795"
     CDS             complement(2581..3054)
                     /locus_tag="VFA35_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HZT55521.1"
                     /translation="MSAAQRFAGAGIASLAAVGAALVSQHVFGMQPCPWCVLQRVIFL
                     AIAIACGIGLVWRGAAGRTLAGGLGALLALGGVAAALWQHFRAAASASCNLTLADTIV
                     SGWLGLDSRWPDVFSARASCADAAVDLLGVPYDVWSLVLFAILGLVLLHTLRRPP"
     gene            complement(3051..>3131)
                     /locus_tag="VFA35_04800"
     CDS             complement(3051..>3131)
                     /locus_tag="VFA35_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZT55522.1"
                     /translation="HARSAFGVAQIPLGACVEIELIAQVQ"
BASE COUNT          504 a         1065 c         1128 g          434 t
ORIGIN      
        1 cgagggcacg gtcgagttcg cgcccaacta cgacggctcg accgaggagc cgcgcctcct
       61 gcccgcgcgc ttgccgttcg tgctcctgaa cggcgcgagc ggcatcgcgg tcgggctcgc
      121 gaccgagatc ccgagccaca acctgcgcga ggtcgcggcc gccgcgatcg cgctgatccg
      181 caacgagatg ctgtcggacg acgagttcgc cacgctgctg cccgggccgg actacccggg
      241 cggcggccag atcatcagca gcgccgaaga gatccgcgcg gcctacgcct cgggccgcgg
      301 cagcctgaag gtgcgcgcga agtggaagat cgaggaactc gcgcgcggcc agtggcagct
      361 cgtcgtcacc gagctgccgc cgggcgcgag cgcgcagaag gtgctcgaag agatcgagga
      421 gctgaccaac ccgaaggtca gggccggcaa gaagacggtc tcgaccgagc aggcacaact
      481 caagacgacc gtgctcgcgg tgctcgacgc cgtgcgcgac gagtcgagca aggacgcgcc
      541 ggtgcgcatc gtgttcgagc cgaagtcgcg cacgaccgcg cagcccgagc tgatcacgac
      601 gctgctcgcc cacacctcgc tcgagagctc ggcgccgatc aatctgacga tgatcggcgc
      661 cgacgggcgg ccgacgcaga agagcctgcg cgagatcctg gccgagtggg tcgcgttccg
      721 cgcggtcacg gtgcagcgcc gcacgcggca ccgcctcgac aaggtgcttg cgcgcatcca
      781 cgtgcttgaa ggccggcaac tggtgctgct gaacatcgac gaggtgatcc gcatcatccg
      841 cgagtccgac gagccgaagg cggcgctgat cgcgcgcttc gatctgagcg agatccaggc
      901 cgacgacatc ctcgacatcc ggctgcgcca gctcgcgcgg ttggaggcga tcaagatcga
      961 gcaggagctg aaggaactgc gcgtcgagca gggcaagctc gaagagattc tgagcagccc
     1021 gagcgcgctg aagcgcacga tgatcaagga gatcgaggcc gacgcgaagg cgcacggcga
     1081 cgatcgccgc accttgatcc aggccgagaa gaaggcggtc gcctcggtgc tcgtgatcga
     1141 cgagccggtc accgtcgtcg tcagcctgaa gggctgggtg cgcgggctga ggggccacga
     1201 ggtcgacaca gccgcgctcg cgttcaaggc gggcgatggt ctgtacggca ccttcgcgtg
     1261 ccgcagcgtc gatgtgctgc tggtgttcgg cagcaacggg cgcgtgtatt cgacgccggt
     1321 cggccaactg ccgggcgggc gcggcgacgg ccagccgatc acctcgctga tcgacctgga
     1381 gagcggcacg cagccggcgc actaccacgc cggccacgcg cagcagaccc tgctgctggc
     1441 gggcaccggc ggcttcgggc tgctcgcgcg cgtcggtgat ctggtcgcgc ggcagcgcgg
     1501 cggcaagagc ttcctgagcc tcgaggtcga cgagaagccg ctgccgccga gcctggccga
     1561 tcagcagacc caggtcgcct gcctgtcgct cgcgggccgc ttgctcgtgt tcgcgctcga
     1621 cgagctgaag ctgcaatcga acggcgggcg cggactgacc ctgatcgacc tcgacgcgaa
     1681 ggacgcgctg gtcagcgtcg ccgcggtcga tcaagccttg cagatcgtcg gcaccggccg
     1741 cggcggcaag ccgaaggacg aggtgctgaa gaacagtgcg ctcgctgagt atgtcgggaa
     1801 gcgtgcacgc aagggcaaga agcaggacgt gatggtcaag gcgatgcgcg tgttgcgcgc
     1861 ggtgcgctga cgcatcggcg tcgaaggtcg ccgcttcaac cggcgacgca gcggttcttg
     1921 tagttcgcct cgaacagctc gagatcggcc ttctcgccgg cgttcatgcc cgccgcgcgt
     1981 tgccgcttgc cgtgcagtat gcgcagcatc tcgtagcact gttcgcggtt gagcacggcg
     2041 cggttgcgcg cgtcgcgctg ggcctggtcg gtggccttgc gctgcgcgcg ctcgtcgctg
     2101 cgatcctggc cgacgcgctg gcgggcctgc tgctcgtctt cactgcagcg cctggcgtag
     2161 ttctcgcgca gctcggacat cgtcgacccg gcaaggccgc gcgccggtcc ggtgcggatg
     2221 ccttcgctga gctgcgcgca ttcggttccc atgtaggcga ggtgctcggg tgccttggcc
     2281 atcgacgggt agtaggtcgg agcgccgcgg gtcggagcct gcaccgggcc gtatgcgccc
     2341 aggcgcgtgt tgccggacgc gacgccgccg ccggtgctgc tgcatggctt gtcggacaga
     2401 tagcttgcgc cggtgccgtt gccgcagcgg tacatggtct gcgccaacag tggtgacgcg
     2461 caagtggcca gcaatgccag cgcggcgccg gccggccaag cggatccgat cctcatctca
     2521 cctccctcgt ccatggcttg ttttctacaa agtgtagcca agccgcagcc ttgggcgctg
     2581 tcagggcggc cgccgcagcg tgtgcagcaa caccagcccg aggatcgcga acagcacgag
     2641 gctccagacg tcgtagggca cgccgagcag gtcgaccgcc gcatcggcgc agctggcgcg
     2701 cgccgagaac acgtcgggcc accggctgtc caggccgagc cagccgctga cgatcgtgtc
     2761 ggccagcgtc aggttgcacg aggccgaggc cgcggcgcgg aagtgctgcc acagtgccgc
     2821 ggcgacgccg ccaagcgcga gcagcgcgcc gagcccgccg gcgagcgtgc gcccggcggc
     2881 gccgcgccag accagcccga tgccgcaagc gatcgcgatc gccagaaaga tgacccgctg
     2941 cagcacgcac caagggcagg gctgcatgcc gaagacgtgc tgcgagacca gcgccgcacc
     3001 gaccgcggcc aggctggcga tgcccgcgcc ggcgaagcgc tgcgctgcgc tcactggacc
     3061 tgcgcgatca gctcgatttc gacgcaggcc ccgagcggga tctgcgccac gccgaacgcg
     3121 ctgcgcgcat g
//
LOCUS       DASRDG010000290         4181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_498968, whole genome shotgun sequence.
ACCESSION   DASRDG010000290 DASRDG010000000
VERSION     DASRDG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498968"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFA35_04805"
     CDS             <1..306
                     /locus_tag="VFA35_04805"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465799.1"
                     /note="catalyzes the formation of arginosuccinate from
                     citrulline and aspartate in arginine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZT55523.1"
                     /translation="ETAQRWVASAITGEVTLELRRGNDYSLLNTESANLTYQPERLSM
                     EKVEGAFSPQDRIGQLTMRNLDIADTRAKLGIYAGTGLLAAGGAADLLRLGKTGADK"
     gene            334..660
                     /locus_tag="VFA35_04810"
     CDS             334..660
                     /locus_tag="VFA35_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55524.1"
                     /translation="MRSFTPVLKNMLARGLLALAFLFAQQTASLHWLSHAVEATQHKN
                     AKAPAADHCDECLALSALGAAAPAHAAALPFAAAQHAPLAAPIAAASQAALRLAFRSR
                     APPILV"
     gene            728..2887
                     /locus_tag="VFA35_04815"
     CDS             728..2887
                     /locus_tag="VFA35_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZT55525.1"
                     /translation="MYKLTTLASAAALAMAGAPARAADEFVAQAGPAPTASASAPAAA
                     QLDRVEVKGTRRRLDDARNALSPETGSTVYRFDKDDIAKLPLGDATPLNQVLLRAPGV
                     VGDSYGQLHVRGDHGNLQYRINGVVIPEAISGFGQALDTRFADSVNVLTGALPAQYGY
                     RTAGVVDIATKGTDLANAGSVDLVLGSRGHREASANIGGSAGDLQYFVTGSILRNDIG
                     IENPSAENNAIHDRTRQTKGFAYLSYLLGEGSRVSLMLGTAQSRFQIPNLTGESPRFS
                     VAGATPPESAQLDANQRERNDFQVLSFQSSLADGTDYQVSAFNRSSRVHYRPDPVGDL
                     AYNGVAADVLRRNVATGLQFDASRVLNAQHTLRAGLFVQRERGAVANASTVFPADGDG
                     NQTSNVPLTIQDDSRLKGHLFGAYLQDEWQPTKALTVNYGLRYDKVDTVVAESQWSPR
                     LGLVYELGRDTRVHAGYARYFTPPPTEKIDTTSVAKFLGTTNALPSDANTAVRSERSN
                     YFDLGISHQLTPRITLGADAYYRDVKHLQDEGQFGNALIYSAFNFERGRIFGIEFSGA
                     YKDERLSAYANLGFSVARGRGIETGQFNFDADELAYIQSHWVHLDHEQRLAASAGVSY
                     RIGDSTTAGGDVLYGSGLRRGFANTEHLPAYVQVNASVAHTVRLDGLGNVDLRISALN
                     LFDRSYQLRDGSGIGVGAPQFAPRRTFYLALSKPFTF"
     gene            2917..3447
                     /locus_tag="VFA35_04820"
     CDS             2917..3447
                     /locus_tag="VFA35_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZT55526.1"
                     /translation="MEPTAADRRSFFGAAAAAAWLLAGLGRPAWALDAASGKVLLTVT
                     GPLANPNDHGAASFDLALLQALPQQSFSTKTPWYAQPRKFTGVALRDLLAAVGAPAGA
                     ALKAVALNDYRVDIPADDVATHGAMLAHLLDDKPMSVREKGPLVIIYPFDDQPELRTA
                     VYYSRAIWQLRSLEIR"
     gene            3444..>4181
                     /locus_tag="VFA35_04825"
     CDS             3444..>4181
                     /locus_tag="VFA35_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55527.1"
                     /translation="MTSGDPRLHPRRVWLLAVVGVLALVLLAVGYVQWRQYKLLNTAS
                     HHQNDALGWSFSQLETEQLRLRNQLERYLDNPGKPDADAVQLRYDIFVSRIGLVDHPR
                     GATIMAGQSAYQPAMVQVRSFVARADRVLGPKPAQPLDAASAASLLEMLDTLNAPLHD
                     LSLGASHLLYERATERNQAVRQQSILGVALTAFQVVLLLTLAFIVLRQFRTLTERRRH
                     LEALADSLSTARVDAEAANRAKSAFLAK"
BASE COUNT          675 a         1572 c         1370 g          564 t
ORIGIN      
        1 gagaccgcgc agcgctgggt cgccagcgcg atcaccggcg aggtcacgct cgagctgcgc
       61 cgcggcaacg actactcgct gctgaacacc gaatcggcca acctcacgta ccagcccgag
      121 cgcctgagca tggagaaggt cgaaggcgcg ttctcgccgc aggaccgcat cggccagctg
      181 acgatgcgca acctcgacat cgccgacacg cgcgccaagc tcggcatcta cgccggcacc
      241 gggctgctcg cggcgggcgg cgcggcggat ctgctgcggc tcggaaagac cggcgcagac
      301 aagtagcgcg ccgaccgacg tagactccgc gcgatgcgtt cgttcacgcc cgtgctgaag
      361 aacatgctcg cccgcggcct gctggcgctg gcgttcctgt tcgcgcagca gacggcgtcg
      421 ctgcactggc tgtcgcatgc ggtcgaggcg acgcagcaca agaacgccaa ggcgccggcc
      481 gccgaccact gcgacgagtg cctcgcgctc agcgcgctcg gcgccgcggc gcccgcccac
      541 gcggcggcgc tgcccttcgc agcggcgcaa cacgcgccgc tcgctgcgcc gatcgcggcg
      601 gcatcccagg ccgcgctgcg gctggcgttc cgttcgagag ccccaccgat cctggtctga
      661 gcccgctcgt gcgagcgcgt gcccggtgca tgcgcttcgc ccttcccgct ttcagatcag
      721 gatcgccatg tacaagttga ccaccctggc cagcgccgcc gcgctcgcca tggccggcgc
      781 acccgcgcgc gcggccgatg aattcgtggc gcaggctggg cctgcgccga ccgcttcggc
      841 cagtgcaccc gcggccgcgc agctcgaccg cgtcgaggtc aagggcacgc gccggcgcct
      901 cgacgacgcc cgcaacgcgc tctcgcccga gaccggcagc acggtctacc gcttcgacaa
      961 ggacgacatc gcgaagctgc cgctcggcga cgcgacgccg ctgaaccagg tgctgctgcg
     1021 cgcgcccggc gtcgtcggcg actcctacgg ccagctgcac gtgcgcggcg accacggcaa
     1081 cctgcagtac cgcatcaacg gcgtcgtcat ccccgaggcc atcagcggtt tcggccaggc
     1141 gctggacacg cgtttcgccg acagcgtgaa cgtgctgacc ggcgcgctgc cggcgcagta
     1201 cggctaccgc accgccggcg tggtcgacat cgcgaccaag ggtaccgatc tggcaaatgc
     1261 cggcagcgtc gacctcgtgc tcggcagccg cggccaccgc gaggccagcg cgaacatcgg
     1321 cggcagcgcc ggcgacctgc agtacttcgt gaccggctcg attctgcgca acgacatcgg
     1381 catcgagaac ccgagcgccg agaacaacgc gatccacgac cgcacgcgcc agaccaaggg
     1441 cttcgcctac ctgagctacc tgctcggcga gggcagccgc gtcagcctga tgctgggcac
     1501 ggcgcaaagc cgcttccaga tcccgaacct gacgggcgaa tcgccgcgct tcagcgtcgc
     1561 cggcgcgacg ccgcccgaat cggcgcaact cgacgcgaac cagcgcgagc gcaacgactt
     1621 ccaggtgctg agcttccagt cgagcctcgc cgacggcacc gattaccagg tgtcggcgtt
     1681 caaccgcagc agccgcgtgc actaccggcc cgacccggtg ggcgacctgg cctacaacgg
     1741 cgtcgccgcc gacgtgctgc ggcgcaacgt ggcgaccggc ctgcagttcg atgcgagccg
     1801 ggtgctgaac gcgcagcaca cgctgcgcgc cggcctgttc gtgcagcgcg agcgcggcgc
     1861 ggtcgcgaac gcatccaccg tgttcccggc cgatggcgac ggcaaccaaa cgagcaatgt
     1921 accgctgacg atccaggacg acagccgctt gaagggccac ctgttcggcg cctacctgca
     1981 ggacgaatgg cagccgacga aggcgctgac cgtcaactac gggctgcgct acgacaaggt
     2041 cgacacggtc gtcgcggagt cgcagtggag cccacgcctc gggctcgtct acgagctcgg
     2101 ccgcgacacc cgcgtgcacg ccggctacgc gcgctacttc acgccgccgc cgaccgagaa
     2161 gatcgacacg acctcggtcg cgaaattcct cggcacgacc aacgcgctgc cgtcggacgc
     2221 gaacaccgcc gtgcgctcgg agcgctcgaa ctacttcgac ctcggcatct cgcaccagct
     2281 gaccccgcgg atcacactcg gcgccgacgc ctactaccgc gacgtgaagc acctgcagga
     2341 cgaaggccag ttcggcaacg cgctgatcta ctcggcgttc aacttcgaac gcggcaggat
     2401 cttcggcatc gagttctcgg gcgcgtacaa ggacgagcgc ctctcggcct acgcaaacct
     2461 cggcttcagc gtggcccgag gccgggggat cgaaaccggc cagttcaact tcgacgccga
     2521 cgagctcgcc tacatccaga gccactgggt gcacctcgac cacgagcaga gactcgccgc
     2581 ctcggccggc gtgtcgtacc gcatcggcga cagcacgaca gccggcggcg atgtgctcta
     2641 cggcagcggc ctgcggcgcg gcttcgcgaa caccgagcac ctgccggcct acgtgcaagt
     2701 gaacgcgtcg gtggcgcaca cggtccggct cgacggcctc ggcaatgtcg acctgcgcat
     2761 ctcggcgctg aacctgttcg accgcagcta ccagttgcgc gacggcagcg gcatcggcgt
     2821 gggcgcaccg cagttcgcgc cgcggcgcac gttctacctg gccttgagca agccgttcac
     2881 gttctgaagc cgcgagcccc gtgacagaat tggcgcatgg agccaaccgc agcggatcga
     2941 cgcagctttt tcggcgccgc tgccgcggcg gcgtggctgc tggccggcct cggccggccg
     3001 gcgtgggcgc tcgacgcggc ctcgggcaag gtgctgctga ccgtcaccgg gccgctcgcg
     3061 aacccgaacg accacggcgc cgcgtcgttc gacctggcgc tgctgcaggc gctgccgcag
     3121 cagagcttct cgaccaagac accgtggtac gcgcagccgc gcaagttcac cggcgtggcg
     3181 ttgcgtgacc tgctcgccgc cgtcggcgca cccgccggtg ccgcgctgaa ggcggtggcg
     3241 ctgaacgact accgcgtcga catccccgcc gacgacgtgg ccacgcacgg cgcgatgctc
     3301 gcgcacctgc tcgacgacaa gccgatgtcg gtacgcgaga aaggcccgct ggtcatcatc
     3361 tacccgttcg acgaccagcc cgagctgcgc accgcggtgt actacagccg cgcgatctgg
     3421 cagctcagaa gcctcgaaat ccgatgacct cgggcgatcc acggctgcac ccccggcgcg
     3481 tctggctgct cgccgtcgtc ggcgtgctgg cgctggtgct gctggcggtc ggctacgtgc
     3541 agtggcgcca gtacaagctg ctcaacaccg cctcgcacca ccagaacgac gcgctcggct
     3601 ggagcttctc gcagctcgag accgagcagc tgcggctgcg caaccaactc gaacgctacc
     3661 tggacaaccc gggcaagccg gatgccgacg cagtgcagct tcgctacgac atcttcgtca
     3721 gccgcatcgg cctggtcgac cacccgcgcg gcgcgaccat catggccggg cagtccgcct
     3781 atcagccggc gatggtgcag gtgcgcagct tcgtcgcgcg cgccgaccgc gtgctcggtc
     3841 ccaagcccgc gcagccgctc gacgcggcct cggccgcgtc gctgctggag atgctcgata
     3901 cgctgaacgc gccgctgcac gacctctcgc tgggcgcctc gcacctgctc tacgagcgcg
     3961 ccaccgagcg caaccaggcg gtgcgccagc agagcatcct gggcgtcgcg ttgaccgcgt
     4021 tccaggtcgt gctgctgctc acgctcgcct tcatcgtact gcgccagttc cgcacgctga
     4081 cggagcggcg gcgccacctc gaggcgttgg ccgacagcct gagcacggcg cgcgtcgacg
     4141 ccgaggccgc gaaccgcgcc aagagcgcct tcctcgccaa a
//
LOCUS       DASRDG010000291         4659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_45351, whole genome shotgun sequence.
ACCESSION   DASRDG010000291 DASRDG010000000
VERSION     DASRDG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45351"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFA35_04830"
     CDS             complement(<1..518)
                     /locus_tag="VFA35_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT55528.1"
                     /translation="MAGDKRVAIVGGGWAGLAAAVEATRRGHRVTLFEMAPQLGGRSR
                     AVDVAGLELDNGQHILIGAYLETLALLRTVGVDVGAAFLRTPLRLTYPDGVGLHLKPG
                     GALLAFGGAVLRQRGWGIGERIALLAAAAGWVVRGFRCDPARTVAQLTQRLPRAIRDE
                     LIDPLCVAALNTP"
     gene            complement(518..1354)
                     /gene="hpnD"
                     /locus_tag="VFA35_04835"
     CDS             complement(518..1354)
                     /gene="hpnD"
                     /locus_tag="VFA35_04835"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HZT55529.1"
                     /translation="MTPEQYVQDKAAKSGSSFYYAFLFLPPPRRAAITAFYAFCREVD
                     DVVDEVSDPSVAAAKLAWWRKEVGAAFAGQPSHPVTQALMPHAAAFDIRAEHLLAVIE
                     GCQMDLEQSRYLDYPGLARYCHLVAGVVGEVAANIFGRTRPETTAYAHKLGQAMQLTN
                     IIRDVGDDARRGRIYLPVSELKQFDVKAQEVLNRGYSERFTALMKFQAERAHRCYDEA
                     FALLPEADRLAQKPGVMMANIYRTLLREIEADDFQVLHQRTSLTPLRKLWIAMRTNWR
                     GR"
     gene            complement(1351..2205)
                     /gene="hpnC"
                     /locus_tag="VFA35_04840"
     CDS             complement(1351..2205)
                     /gene="hpnC"
                     /locus_tag="VFA35_04840"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HZT55530.1"
                     /translation="MSVDHYENFPVASLLCPPALRPAVAAIYHFARTADDIADEGDAP
                     PAQRLADLAAYRADLAAACNGDAISARWPQVFDPLRTMLAGHALPVELLGDLLDAFEQ
                     DVVKTRYADRAELLDYCRRSANPVGRLLLHLYGVGDAQALRRSDAICTALQLANFWQD
                     LGVDTRRGRLYAPASDCARSGVDAAALLAQRDAPNVRALVGELVGWTRELMLAGAPLV
                     HAIPGRAGWELRLVVQGGLRILEKIAALDFATLQSRPKLHWHDAPLLLWRALWMRAPR
                     AVVASTSA"
     gene            2327..3466
                     /locus_tag="VFA35_04845"
     CDS             2327..3466
                     /locus_tag="VFA35_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZT55531.1"
                     /translation="MRHALLVTLAAVAALAACSKTEPAPDPVRAVRTLTVSPQTTAGA
                     YEYAGEVRARTESRLSFRVGGKMLKRLVNLGDTVKAGQLLAQLDPQDLRLGQDAARAT
                     VAAAQASHEQSAADFKRYQELAEKGFIGPAELERREMAMRTARAQLDQARAQAGVQGN
                     QAGYASLVADAPGVITGTELEPGMVAAAGTPVLRLAHDGPRDVVFSVPEDKVTIVKAQ
                     AAVPGRFTVRLWGEGGAPLPATIREIAAAADPVTRTFLVKADIGNAAASGVRLGQTAS
                     VSMALPQLAGVTKLPLSALREAQGRSAVWLVDRGSMTVKSQDVQLAGADGNEAVITAG
                     LAPGQIVVTAGVHVLSPGQKVKFYVDPGLAPASASAPVATPVGVK"
     gene            3517..>4659
                     /locus_tag="VFA35_04850"
     CDS             3517..>4659
                     /locus_tag="VFA35_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT55532.1"
                     /translation="MSRFNISKWALDHAALTRYLLVVLLVLGAAAYFQLGQDEDPPFT
                     FRAMALRVFWPGATAQQVAEQVIDKLEKTLQEVPHADKIRSYSKPGEALIIFQVRDSS
                     PPKEMAQIWYTVRKKIGDMRATLPAGVIGPYFNDEFGDVYGSIYALSADGFSYQELKD
                     QADRVRQRLLKVQDVSKVGIFGAQDEKLFVEIPQRRLAELGLDFNQVLAQLGQQNAVE
                     SAGVVNAPTDFVQVRVGGQFNTVDELRQFPIRANGASFKLGDIASVRRGYVDPPQVKV
                     RHQGAEVIALGISMARGGDIVRMGKALDATVAQIRAELPAGIELHQFQNQSKVVTSSV
                     NEFVGTLIEAVVIVLGVSFVALGLHFKPFRLDWRPGLVVGITIPLVL"
BASE COUNT          692 a         1632 c         1678 g          657 t
ORIGIN      
        1 ggcgtgttca gcgccgcgac gcacagcggg tcgatcagct cgtcgcggat cgcccggggc
       61 aggcgctgcg tcagctgcgc gaccgtccgt gccggatcgc agcgaaaccc acgcacgacc
      121 cagcccgcgg ccgcggcgag cagcgcgatg cgttcgccga tgccccagcc gcgctggcgc
      181 agcaccgcgc cgccgaacgc gagcagcgcc ccgccgggct tcaggtgcag cccgacgccg
      241 tcgggatagg tcagccgcag cggcgtgcgc aggaacgctg cgcccacgtc gacgccgacc
      301 gtgcgcagca gcgcgagcgt ctcgagatac gcgccgatca ggatgtgctg gccgttgtcg
      361 agttccaggc ccgcgacgtc gaccgcgcgc gagcggccgc cgagctgcgg cgccatctcg
      421 aacagcgtga cgcggtggcc gcgccgtgtc gcctcgaccg ccgccgccaa gccggcccag
      481 ccgccgccga cgatggcgac gcgcttgtcg cccgccacta gcgcccgcgc cagttcgtgc
      541 gcatcgcgat ccagagcttg cgcaacggcg tcagcgaggt gcgctggtgc agcacctgga
      601 agtcgtcggc ctcgatctcg cgcagcaagg tgcggtagat gttggccatc atcacgcccg
      661 gcttctgcgc cagccggtcg gcctcgggca gcagcgcgaa ggcctcgtcg tagcagcggt
      721 gcgcgcgttc ggcctggaac ttcatcagcg cggtgaagcg ctcgctgtag ccgcggttga
      781 gcacttcctg cgccttcacg tcgaactgct tcagctccga gacgggcagg tagatgcggc
      841 cgcggcgcgc atcgtcgccg acgtcgcgga tgatgttggt caactgcatc gcctggccca
      901 gcttgtgcgc gtaggcggtg gtctcgggcc gcgtgcggcc gaagatgttc gccgcgacct
      961 cgccgaccac gccggccacc aggtggcagt agcgcgcgag gccggggtag tcgaggtagc
     1021 gcgattgctc caggtccatc tggcagcctt cgatcaccgc gagcaggtgc tcggcgcgga
     1081 tgtcgaacgc ggcggcgtgc ggcatcagcg cctgggtcac cgggtggctg ggctggccgg
     1141 caaaggccgc gccaacctct ttgcgccacc acgcgagttt ggccgcggcc accgacggat
     1201 cgctgacctc gtcgaccacg tcgtcgacct cgcggcagaa cgcgtagaac gcagtgatcg
     1261 cggcgcggcg cggcggcggc aggaagagaa aagcgtagta gaagctcgag ccgctcttgg
     1321 cggccttgtc ttgcacgtac tgctcggggg tcatgcggat gtgctcgcga cgacggcacg
     1381 cggcgcgcgc atccacagcg cgcgccagag caacagcggc gcgtcgtgcc aatgcagctt
     1441 cgggcggctt tgcagcgtgg cgaagtccag cgcggcgatc ttctccagga tgcgcagccc
     1501 gccctgcacg acgaggcgca gctcccagcc ggcgcggccc gggatcgcgt gcaccagcgg
     1561 cgcgccggcg agcatcagct cgcgggtcca gccgaccagc tcgccgacca gcgcgcgcac
     1621 gttcggcgcg tcgcgttgcg cgagcagcgc agcggcgtcg acgccgctgc gcgcgcagtc
     1681 gctcgccggc gcgtagaggc gaccgcggcg ggtgtcgacg cccaggtcct gccagaagtt
     1741 cgcgagctgc agcgcggtgc agatcgcatc ggatcggcgc agcgcctgcg cgtcgccgac
     1801 gccgtacagg tgcaagagca ggcggcccac cgggttggcc gagcggcggc agtagtcgag
     1861 cagctcggcg cggtcggcgt agcgggtctt gacgacgtcc tgctcgaacg cgtcgagcag
     1921 atcgcccagc agctcgaccg gcagcgcgtg gccggccagc atcgtgcgta gcgggtcgaa
     1981 cacctgcggc cagcgcgccg agatggcatc gccattgcat gcggcggcca gatcggcgcg
     2041 gtacgccgcc aggtcggcca ggcgttgcgc gggcggtgca tcgccctcgt cggcgatgtc
     2101 gtcggccgtg cgcgcaaagt gatagatcgc ggccacggcc ggccgcagcg ctggcggaca
     2161 cagcagcgat gccacgggga agttctcgta atggtcgacg ctcacggcga cgattgtcca
     2221 cgttgtgcgg gcggcgggcc ggcgccggcc gcccacgggg tttgacaggt gcagactcgc
     2281 cgaatacatt actgaccggt cagtcattta tatgccgagg actcccatgc gtcacgcgct
     2341 gctcgtcacc ctggccgctg ttgcggcgct cgcggcttgc tcgaagaccg agcccgcgcc
     2401 cgatccggtg cgcgcggtcc gcacgctgac ggtctcgccg caaaccaccg ccggtgcgta
     2461 cgagtatgcc ggcgaggtca gggcgcgcac cgagtcacgc ctgagcttcc gcgtcggcgg
     2521 caagatgctg aagcgcctgg tcaacctcgg cgacacggtc aaggccggcc agttgctcgc
     2581 gcagctcgac ccgcaggact tgcggctggg acaagacgcc gcccgcgcca cggtcgccgc
     2641 cgcgcaggcc agccacgagc agagcgcggc cgacttcaag cgctaccagg agcttgccga
     2701 gaagggcttc atcggcccgg ccgagctcga gcgccgcgag atggcgatgc ggactgctcg
     2761 cgcccagctc gaccaggccc gggcccaggc cggcgtgcag ggcaaccagg ccggctacgc
     2821 atcgctggtg gccgatgccc cgggcgtgat caccggcacc gagctcgagc ccggcatggt
     2881 cgccgcggcc ggcacgccgg tgctgcgctt ggcccacgat ggcccgcgcg acgtggtgtt
     2941 ctcggtgccc gaagacaagg tcaccatcgt caaggcgcag gccgccgtgc cgggccgctt
     3001 cacggtgcgg ctgtggggcg agggcggcgc gccgctgccg gcgacgattc gcgagatcgc
     3061 ggccgcggcc gacccggtca cgcgcacctt cctggtcaag gccgacatcg gcaacgcggc
     3121 cgcgagcggc gtgcgcctcg gccagaccgc gagcgtgtcg atggcgttgc cgcagttggc
     3181 cggcgtgacc aagctgccgc tgtcggcgtt gcgtgaagcg cagggccgca gcgcggtctg
     3241 gctggtcgat cgcggcagca tgacggtcaa gtcgcaagac gtgcagctcg ccggcgccga
     3301 cggcaacgag gccgtgatca cggccggtct tgcacccggg cagatcgtcg tgaccgccgg
     3361 cgtgcacgtg ctgagcccgg ggcagaaggt gaagttctac gtcgacccgg ggctggcgcc
     3421 ggcttccgcg tccgctcccg tcgccacacc ggtcggcgtc aagtgagcgc cgacgcgaac
     3481 ggcgccggac acgccgcacc ggctgacagc ggcgcgatct cgcgcttcaa catctcgaag
     3541 tgggcgctcg accatgccgc gctgacccgc tacctgctcg tcgtgctgct cgtgctcggc
     3601 gcggccgcgt acttccagct cgggcaggac gaagacccgc cgttcacctt ccgcgcgatg
     3661 gcgctgcgcg tgttctggcc cggcgcgacc gcgcagcagg tcgccgagca ggtcatcgac
     3721 aagctcgaga agacgctgca ggaggtgccg cacgccgaca agatccgcag ctactccaag
     3781 cccggcgagg ccttgatcat cttccaggtg cgcgactcgt cgccgcccaa ggagatggcg
     3841 cagatctggt acacggtgcg caagaagatc ggcgacatgc gggcgacgct gccggccggc
     3901 gtgatcggcc cgtacttcaa cgacgagttc ggcgacgtct acggctcgat ctacgcgctc
     3961 tcggccgacg gcttcagcta ccaggagctg aaggaccagg ccgaccgcgt gcgccagcgc
     4021 ctgctcaagg tgcaggacgt gagcaaggtc ggcatcttcg gcgcccagga cgagaagctt
     4081 ttcgtcgaga tcccgcagcg caggctcgcc gagctggggc tcgacttcaa ccaggtgctc
     4141 gcgcaactcg gccagcaaaa cgcggtcgag tcggccggcg tcgtcaacgc gccgaccgac
     4201 ttcgtgcagg tgcgcgtcgg cggccagttc aacacggttg acgaactgcg ccaattcccg
     4261 atccgcgcga acggcgcgag cttcaagctc ggtgacatcg cctcggtgcg ccgcgggtac
     4321 gtcgacccgc ctcaggtgaa ggtgcggcac cagggcgccg aggtcatcgc gctcggtatc
     4381 tcgatggccc ggggcggcga catcgtgcgc atgggcaagg cgctggatgc cacggtcgcg
     4441 cagattcgcg ccgagctgcc cgcgggcatc gaactgcatc agttccagaa ccagtcgaag
     4501 gtggtgacca gttcggtcaa cgagttcgtc ggcacgctga tcgaggcggt ggtgatcgtg
     4561 ctcggggtca gcttcgtcgc gctcggcctg cacttcaagc ctttccggct cgactggcgg
     4621 ccggggctgg tcgtcggcat cacgatcccg ctggtgctc
//
LOCUS       DASRDG010000292         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_157618, whole genome shotgun sequence.
ACCESSION   DASRDG010000292 DASRDG010000000
VERSION     DASRDG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157618"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="VFA35_04855"
     CDS             <1..1525
                     /locus_tag="VFA35_04855"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZT55533.1"
                     /translation="KAVLGKLLFWEEQMSSNNRVACGTCHRPAQGGGDPRRAPNPGLD
                     GVQPSPDDTFGSPGIIRSDAANSYLPSSLFGLQPQVTGRAAPSAINGAWFGEAFWDGR
                     ARGQFLDPETGLVSLPAGGALESQATGPVLSGVEMAHDARNWTEVEQKLQTARPMAVA
                     TNLPPDMAAAIAGGRSYPDLFNAAFGDPAITAARIAFAIATYERTLVPDQTPFDQFVR
                     GNPAALSPQQVNGMNLFNGPARCNLCHTPGLFSDGGFHNLGLRPIAEDSGRLAVSGLF
                     GDRGRFKTPSLRNAGLRSSFMHTGQFTQLGQVLGFYNGGGGPNLDNKDPLLQPLGLQP
                     NLLGDLGNFIANGLTDARVRDGLFPFDRPTLASERIPPAGFLFGPGSPGTGGRVPQIL
                     AEVPANVGNVDFKLGVANAVGGAASTLVVALQRAPIGAQLNGINVDVDIHVTPALVGV
                     LLNGPPGAAGAGYGTFAVALPALPGLVGITVFAQWFVWDTGVPAGGASTQGAEVRFF"
     gene            1575..>2291
                     /locus_tag="VFA35_04860"
     CDS             1575..>2291
                     /locus_tag="VFA35_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55534.1"
                     /translation="MDPGGDRQERPAAPDASQIADALATAIKACAVWPRRNPRVQQSS
                     AALAALLRECIEHPGVLEVRLRGSELLVGRERASPSLVVGWLIARFRDVGLAGLELAA
                     GVDREALIDFGTALARAGRQKTDCTLDWPARHPTLRPLDLVFEGAHSAEGGGTGAGTS
                     MNDVLRRQLIETLHESAAIRSRLRSMHDTLAGESRGAIALDVLACVVEALPIEIARDA
                     DLARAAVERVLAETHVGAMAQ"
BASE COUNT          307 a          853 c          829 g          302 t
ORIGIN      
        1 caaggcggtg ctcggcaagc tgctgttctg ggaggagcag atgtcgagca acaaccgcgt
       61 cgcctgcggc acctgccacc ggcctgccca gggcggcggc gatccgcggc gcgcgccgaa
      121 cccgggactc gacggcgtgc agccgtcgcc cgacgacacg ttcggctcgc cgggcatcat
      181 ccgcagcgac gccgcgaaca gctacctgcc gagctcgctg ttcggcctgc agccgcaggt
      241 gacgggccgc gcggcgccga gcgcgatcaa cggcgcgtgg ttcggcgaag cattctggga
      301 cgggcgcgcg cgcggccagt tcctcgatcc cgagacgggg ctcgtgagcc tgcctgccgg
      361 cggcgcgctc gagagccagg ccacaggccc ggtcctgagc ggcgtcgaga tggcgcacga
      421 cgcgcgcaac tggacggagg tcgagcagaa gctgcagacg gcgcggccga tggcggtcgc
      481 gaccaacctg ccgcccgaca tggcggcggc gatcgcgggc ggccgctcgt atcccgatct
      541 gttcaacgcc gcgttcggcg atcccgcgat caccgccgcg cgcatcgcgt tcgcgatcgc
      601 gacctacgag cgcacgctgg tgcccgacca gacgccgttc gaccagttcg tgcgcggcaa
      661 ccccgcggcg ctgtcgccgc agcaggtgaa cggcatgaac ctgttcaacg gccccgcgcg
      721 ctgcaacctc tgccacacgc ccggactgtt cagcgacggc gggttccaca acctcggcct
      781 gcggccgatc gcggaggaca gcggtcgcct ggcggtcagc ggcctgttcg gcgaccgcgg
      841 ccgcttcaag acgccgagcc tgcgcaacgc cggcctgcgg tcgagcttca tgcacacggg
      901 ccagttcacg cagctcggcc aggtcctcgg cttctacaac ggcggcggcg gccccaacct
      961 cgacaacaag gatccgctgc tgcaaccgct cggcctgcag ccgaacctgc tcggcgacct
     1021 cggcaacttc atcgccaacg gcctcaccga cgcgcgcgtg cgcgacggcc tgttcccgtt
     1081 cgaccggccg acgctcgcca gcgagcgcat cccgcccgcg ggcttcctgt tcggccccgg
     1141 ttcgccgggc accggcggcc gcgtgccgca gatcctcgcc gaggtgcccg ccaacgtcgg
     1201 caacgtcgac ttcaagctcg gcgtcgccaa tgcggtcggc ggcgcggcgt cgacgctggt
     1261 ggtcgcgctg cagcgcgcgc cgatcggcgc gcagctcaac ggcatcaacg tcgacgtcga
     1321 catccacgtg acgcccgcgc tcgtcggcgt gctgctcaac gggccccccg gcgcggccgg
     1381 cgccggctac ggcacgttcg cggtcgcact gcctgctttg ccgggcctcg tcggcatcac
     1441 ggtgttcgcg cagtggttcg tctgggacac cggcgtgccg gccggcggcg cgagcacgca
     1501 gggggccgaa gtccgcttct tctgaccggt tctcctgaac tggacgctcc cgtgctgccg
     1561 atgagtccgg gcgaatggac ccgggcggcg acagacagga gcggccggcg gctcccgatg
     1621 cctcgcagat cgccgatgcg ctggcgacgg cgatcaaggc gtgcgcggtg tggccgcgca
     1681 ggaacccccg cgtgcagcag agcagcgccg cgctggccgc gctgctgcga gagtgcatcg
     1741 agcaccccgg cgtgctcgag gtgcgcctgc gcggcagcga gctgctggtc ggccgcgaac
     1801 gcgcgtcccc gtcgctggtc gtcggctggc tgatcgcgcg gttccgcgac gtcgggctcg
     1861 ccggcctcga actcgcggcg ggcgtcgacc gcgaggcgct gatcgacttc ggcaccgcac
     1921 tcgcgcgcgc gggccgtcag aaaaccgatt gcacgctcga ctggccggcg cggcatccga
     1981 cgctgcgccc gctcgacctc gtgttcgagg gcgcgcactc ggccgaaggc ggcggcacgg
     2041 gcgcgggcac gagcatgaac gacgtgctgc ggcggcagct gatcgagacg ctgcacgagt
     2101 cggcggcgat ccgttcgcgg ctgcgcagca tgcacgacac cctcgcgggc gagtcgcgcg
     2161 gcgcgatcgc gctcgacgtg ctcgcgtgcg tggtcgaggc gctgccgatc gagatcgcgc
     2221 gcgacgccga cctcgcgcgc gccgcggtcg aacgcgtgct ggccgagacg cacgtcggcg
     2281 cgatggcgca g
//
LOCUS       DASRDG010000293         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1649962, whole genome shotgun sequence.
ACCESSION   DASRDG010000293 DASRDG010000000
VERSION     DASRDG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649962"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFA35_04865"
     CDS             <1..372
                     /locus_tag="VFA35_04865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZT55535.1"
                     /translation="PIADTNDFLLIEATPGRAVFQGRPLLRHYNPLGTVHGGWIATLL
                     DSCVGCAVHASLPAGKAYTTAELKVNYVRALTERVGLVRAIGSVIHLGNRMATAEGRL
                     VGPDGKLYAHASTTCFIFDTP"
     gene            369..758
                     /gene="gloA"
                     /locus_tag="VFA35_04870"
     CDS             369..758
                     /gene="gloA"
                     /locus_tag="VFA35_04870"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HZT55536.1"
                     /translation="MRLLHTMLRVGDLDRSIRFYTDVMGMTLLRQSENPSQHYSLAFL
                     GYGKNPEHAEIELTYNHGVSSYELGTAYGHIAIGVPDVAAACEKIRRGGGNVTREAGP
                     VKGGTTVIAFVTDPDGYKIELIERPNH"
     gene            767..2068
                     /locus_tag="VFA35_04875"
     CDS             767..2068
                     /locus_tag="VFA35_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT55537.1"
                     /translation="MLAQLVSGVLDRRGLHYAWVIAAVTFLAMLTTSAALGLPGAMLQ
                     PLAREFGWTTDQLSSVFALRFALFGLLGPFAAVFIGRFGLRTIMVTAATFIATAMVLA
                     TQVTQLWQLFLLWGLVLGCGTGLTALVLGAMVANRWFTSNRGLVVGLFAASTATGQLL
                     FLPLAAWMIEHLGWRYAVIPVFAACVVVGLLATLLMRDHPGSIGLRAFGEPETAPIEA
                     PPARAATLNFLGPFQVLAAAARNRTFWILAGTFFVCGLSTNGLLQTHFISLCGDNGMT
                     AVPAASVLAMMGAFDLVGTTLSGYLSDRYDNRKLLFWYYGLRGLSLFWLPFSTFTLYG
                     LSIFAMFYGLDWIATVPPTVKLAAQEFGKEKAGMIFGWVFAAHQLGAATAAYGAGLTR
                     TLLLTYNPALFAAGAACLIAAAAVLLIRLQARPAAVAVAAC"
     gene            complement(2082..>3740)
                     /locus_tag="VFA35_04880"
     CDS             complement(2082..>3740)
                     /locus_tag="VFA35_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55538.1"
                     /translation="ELAKRALDYYRRLPAELRSPETERNQALAQVRYGTVLRVQSRID
                     EARAALGTAVTALEALRARGDRSEAAAIGLALGLTEQSRAVTPAGYDRDAVPPAERAA
                     ALLAPAAAASGASVALRRAHAAALTQLGFVQTRQRRFEPGLAALEAALATWRGIDPLL
                     AESDAAVNFGVATARLVEAYDRANRPEDAARAGEEGLRATSTLIERQPSNMLALRTRA
                     LILDSLAAVEWNQLQPGRRLAAANDAARDWALLSRIDPSDAVLKNSLMNARTIASAAL
                     WDQGRPRQSLAKVLENDEFGPAAEGSYVVASALYYSIDSAAYLAAELGQAVAADKHLV
                     DRQHLFDISMRSNGPPSFLLQYFRIQTRIRPVESAMLLGDLTRARAAAQGLRERLLAL
                     PTTNDFERLRMAAALNQLHNTLGRVALQARDFAAAQAEFRLVAETRKQLPAETLVDRR
                     DAADDASLLAITLAHAGRLDEARALAEPALALQREVHARQTDDQMHKLGLVLALVAAA
                     RATPDKASALLAQAQAAFDSLPAEARGLRSSQLLQGLIADAPRP"
BASE COUNT          508 a         1328 c         1287 g          617 t
ORIGIN      
        1 ccgatcgccg acacgaacga cttcctcctg atcgaggcca cgccgggccg cgccgtgttc
       61 cagggccggc cgctgctgcg ccactacaac ccgctcggca cggtgcacgg cggctggatc
      121 gcgacgctgc tggattcgtg cgtcggctgc gcggtgcacg cctcgctgcc ggcgggcaag
      181 gcctacacga ccgccgagct caaggtgaac tacgtgcgcg cgctcaccga gcgggtcggc
      241 ctggtgcgcg cgatcggcag cgtgatccac ctcggcaacc gcatggcgac ggccgagggc
      301 cgcctcgtcg gcccggacgg caagctgtac gctcacgcct ccaccacctg cttcatcttc
      361 gacaccccat gagactgctc catacgatgc tgcgcgtcgg cgacctcgat cgctcgatcc
      421 gcttctacac cgacgtgatg ggcatgacgc tgctgcgcca gtccgagaac ccgtcgcagc
      481 actactcgct cgcctttctc ggctacggca agaaccccga gcacgccgag atcgagctga
      541 cctacaacca cggcgtatcg tcgtatgagc tgggcaccgc ctacggccac attgcgatcg
      601 gcgtgcccga tgtcgcggcg gcctgcgaga agatccgccg cggcggcggc aacgtcacgc
      661 gcgaggccgg cccggtcaag ggcggcacga ccgtgatcgc cttcgtcacc gaccccgacg
      721 gctacaagat cgaattgatc gaacgcccga atcactgagt cgccgcatgc tcgcccaact
      781 cgtttccggc gtgctcgacc ggcgcggcct ccactacgcg tgggtgatcg ccgcggtcac
      841 cttcctcgcg atgctgacga cctcggccgc gctcggcctg ccgggggcga tgctgcagcc
      901 gctcgcccgc gagttcggct ggaccaccga ccagctctcg tcggtgttcg cgctgcgctt
      961 cgcgctgttc gggctgctcg ggccgttcgc ggcggtgttc atcgggcgct tcgggctgcg
     1021 cacgatcatg gtcaccgcgg ccacgttcat cgccacggcg atggtgctgg ccacgcaggt
     1081 gacgcagctc tggcagctgt tcctgctgtg gggcctggtg ctcggttgcg gcaccggcct
     1141 gacggcgctg gtgctcggcg cgatggtcgc gaaccgctgg ttcacctcga accgcgggct
     1201 ggtggtcggc ctgttcgccg cgagcaccgc gaccggccag ttgctgttcc tgccgctcgc
     1261 cgcgtggatg atcgagcacc tgggctggcg ctacgcggtg atcccggtgt tcgccgcctg
     1321 cgtggtcgtc gggctgctcg cgacgctgct gatgcgcgac cacccgggca gcatcggcct
     1381 gcgcgcgttc ggcgagcccg agacggcacc gatcgaggcg ccgccggcgc gcgccgcgac
     1441 gctgaacttc ctcggcccgt tccaggtgct cgccgcggcg gcgcgcaacc gcacgttctg
     1501 gatcctcgcc ggcaccttct tcgtctgcgg cctcagcacc aacggcctgc tgcagaccca
     1561 cttcatctcg ctgtgcggcg acaacggcat gacggccgtg ccggcggcct cggtgctggc
     1621 gatgatgggc gcgttcgacc tcgtcggcac gacgctgtcg ggctacctct ccgaccgcta
     1681 cgacaaccgc aagctgctgt tctggtacta cggcttgcgc gggctgtcgc tgttctggct
     1741 accgttctcg accttcacgc tctatggcct gtcgatcttc gcgatgttct atggcctgga
     1801 ctggatcgcc accgtgccgc cgaccgtcaa gctcgccgcg caggagttcg gcaaagagaa
     1861 ggccggcatg atcttcggct gggtcttcgc cgcgcaccag ctcggcgccg cgaccgcggc
     1921 ctacggcgcg ggcctgacgc gcacgctgct gctgacctac aacccggcgc tgttcgcggc
     1981 cggcgccgcc tgcctgatcg cggcggcggc ggtactgctg atccggctgc aggcgagacc
     2041 ggcagcggta gcggtggccg cctgctgatc accgagccgg atcaaggccg cggcgcgtcg
     2101 gcgatcagac cttgcaggag ctggctgctg cgcaggccgc gcgcctcggc cggcaggctg
     2161 tcgaacgcgg cctgggcctg cgcgagcagg gcgctcgcct tgtcgggcgt ggcccgcgcc
     2221 gcggccacca gcgcgagcac caagccgagc ttgtgcatct ggtcatcggt ctggcgcgcg
     2281 tgaacctccc gctgcaaggc cagcgccggc tcggccaacg cacgcgcttc gtcgagtcgc
     2341 ccggcatggg ccagggtgat cgccagcagc gatgcatcgt cggccgcatc gcgccggtca
     2401 acgagggttt cggccggcag ttgtttgcgg gtttcggcga cgagacggaa ctcggcttgc
     2461 gccgcagcga aatcccgggc ctgcaatgcc acccggccca gggtgttgtg cagttggttc
     2521 aatgcggcgg ccatgcgcag ccgctcgaag tcattcgtgg tcggcaacgc caacagccgc
     2581 tcgcgcaggc cctgggcagc agcacgcgcg cgcgtcaggt cgcccaagag catcgccgac
     2641 tccacgggtc tgatcctggt ttgtatgcgg aagtattgga ggagaaagga cggggggcca
     2701 ttgctgcgca tgctgatgtc gaagagatgc tgcctgtcga caaggtgctt gtcggccgcc
     2761 acggcttggc cgagctcagc ggcgaggtag gcggccgagt cgatgctgta atagagtgcg
     2821 ctggcgacca cgtacgagcc ctcggcagca ggcccgaact catcgttctc gagaaccttg
     2881 gccaggcttt ggcgtgggcg accctgatcc cacaaggcag cggacgctat cgtgcgggcg
     2941 ttcatcaggc tgttcttcaa gaccgcgtca ctcgggtcga tgcgcgacag caaggcccag
     3001 tcacgcgccg catcgttggc tgcggccaaa cggcgccccg gctgcaattg attccactcc
     3061 acggcggcca gagaatccaa aatcaaggcg cgcgtgcgca aggccagcat gttgctcggc
     3121 tgcctctcga tcagcgtcga tgtcgcgcgc aggccctctt cgccggcgcg ggcggcgtct
     3181 tcaggtcgat tcgccctgtc gtaggcctcg accagccggg ccgtcgcgac accgaagttg
     3241 accgccgcgt cgctctcggc cagcagcggg tcgatgccgc gccatgtggc cagcgccgcc
     3301 tccagtgccg ccaggccggg ttcgaagcgc cgctgccgtg tttgcacgaa cccaagttgc
     3361 gtcagcgctg ccgcgtgggc gcgccgcaag gcgacgctcg cgccgctggc cgcggcggcc
     3421 ggcgcgagca gcgccgcggc ccgctcggcc ggtggcacgg cgtcgcggtc gtagcccgcc
     3481 ggggtcactg cacgcgattg ctcggtcagc ccgagtgcga gaccgatcgc ggcagcttcg
     3541 ctgcgatcac cgcgggcgcg cagtgcttcc agggccgtga cggcggtgcc gagtgccgcc
     3601 cgcgcctcgt cgatccggct ttgaacacgc agcacggtgc cgtagcgcac ttgcgcgagt
     3661 gcctggttgc gctcggtctc gggtgagcgc aactcggcgg gcaggcggcg gtagtagtcg
     3721 agcgcgcgct tggccagttc
//
LOCUS       DASRDG010000294         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1426955, whole genome shotgun sequence.
ACCESSION   DASRDG010000294 DASRDG010000000
VERSION     DASRDG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426955"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /locus_tag="VFA35_04885"
     CDS             <1..98
                     /locus_tag="VFA35_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT55539.1"
                     /translation="RTTPRFVELHQAIWDVLREEVLAGYRQQLAA"
     gene            118..921
                     /locus_tag="VFA35_04890"
     CDS             118..921
                     /locus_tag="VFA35_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55540.1"
                     /translation="MRYLRLWQVGLLVVVFVVWHLLTTPGLLPTFVFENDRQAAFFFG
                     EPLKIFDRIWQWFIGHADIYGHLWITLVETVLAFAIGTVLGLGFGLWLALSPLAGAIA
                     DPYIKALNSMPRVILAPIFAVWFGLGVASKVALGVTLVFFIVFFNVYQGVKEVSPVVL
                     ANARMLGASQRDLLRHVYLPSATSWVFSSLHTSVGLAFVGAVVGEYLGSSRGVGYLIL
                     QAEGAFDINTVMAGILVLTVFALALDAAVGRIERRLMKWQPQAGETEKI"
     gene            1022..1465
                     /locus_tag="VFA35_04895"
     CDS             1022..1465
                     /locus_tag="VFA35_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55541.1"
                     /translation="MNAEARIALGLGLALIVTCAALPQTTSAVPEASPQPAVQRAGSV
                     EYLNGGAGEEERATMSAQRSAFPLRIVFSQPGGAYAVADHVDVSQGTARVLEVDNAGP
                     ILMLKLAPGDYAVDARYAGKTERRQVRVGRDGTQLDWRLPEEPRR"
     gene            complement(1495..>2484)
                     /gene="mmsA"
                     /locus_tag="VFA35_04900"
     CDS             complement(1495..>2484)
                     /gene="mmsA"
                     /locus_tag="VFA35_04900"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZT55542.1"
                     /translation="SFILKPSERDPSPSIFMAELLQEAGLPKGVFNVVQRDRVAVEAL
                     LVHPDVKALSFVGSTPIARYIYETGARHGKRVQALGGAKNHMVVMPDANLEQAVDALI
                     GAAYGSAGERCMAISVAVLVGDVADRIVPLLAERATKLVVKNGMETDAEMGPLVTREA
                     LQRVESYIASGVADGATLAADGRGHAVAGHEQGFFTGGTLFDHVTPQMKIYREEIFGP
                     VLSCVRVPDFEAALKLINEHEFGNGVACFTSDGNAAREFARRVEIGMVGINVPIPVPM
                     AWHGFGGWKSSMFGDTHAYGEEGVRFYTRQKSVMQRWPASTPKGAEFTMPTSK"
BASE COUNT          357 a          872 c          843 g          412 t
ORIGIN      
        1 tgcgaacgac gccgcgcttc gtggagctgc accaggcgat ctgggacgtg ctgcgcgagg
       61 aggtgcttgc cggctaccgg cagcagcttg cggcatgacg accccgggcg ccgcggcatg
      121 agatacctgc gcctctggca ggtcgggctg ctcgtcgtcg tcttcgtcgt ctggcacctg
      181 ctgacgacgc caggcctctt gcccaccttc gtgttcgaga acgaccgcca ggccgcgttc
      241 ttctttggcg agccgctgaa gatcttcgac cggatctggc aatggttcat cggccatgcc
      301 gacatctacg gccacctctg gatcaccctg gtcgagacgg tgctggcctt cgccatcggc
      361 accgtgctcg gcctgggctt cggcctctgg ctggctctca gccccctggc cggagcgatc
      421 gccgacccgt acatcaaggc actgaacagc atgccgcggg tgatcctggc gccgatcttc
      481 gccgtctggt tcggcctcgg cgtggcaagc aaggtcgcgc tcggcgtgac cctggtgttc
      541 ttcatcgtct tcttcaacgt ctaccagggc gtcaaggagg tgagcccggt ggtgctcgcc
      601 aacgcgcgca tgctcggcgc ctcgcagcgc gacctgctgc gccacgtcta cctgccgagc
      661 gcgacgagct gggtgttcag ctcgctgcac acctcggtgg gcctggcgtt cgtcggtgcg
      721 gtggtcggcg aatacctcgg ctcctcgcgc ggcgtcggct acctgatcct gcaggccgag
      781 ggcgccttcg acatcaacac cgtgatggcc gggatcctgg tcctgacggt gttcgcgctc
      841 gccctcgacg cggcggtcgg gcggatcgag cgccgcctga tgaaatggca gccgcaggcg
      901 ggagagaccg agaagatcta gactggtcgg acagctccga caggttcgcg cgggcggcgc
      961 cgatggtgcc gccgcttcgc gtcgcttacc ttcaggcgat cctcatccca tcgacagaag
     1021 catgaatgcc gaagcacgca tcgctctggg cctcggtctc gcgctgatcg tcacctgcgc
     1081 cgcgctgccg cagacgacgt ccgccgtgcc ggaggcgtcg ccacagcccg cggtgcagcg
     1141 cgccggcagc gtcgaatacc tcaacggcgg cgccggcgag gaagagcgtg ccaccatgtc
     1201 ggcccagcgc agcgcgtttc cgttgcgcat cgtcttcagc cagccgggcg gcgcgtatgc
     1261 cgtcgccgat catgtcgacg tgagccaggg cactgccagg gtgctcgagg tcgacaacgc
     1321 ggggccgatc ctgatgctga agctggcccc gggcgactac gcggtcgatg cccgttatgc
     1381 gggcaagacc gagcgccgcc aggtgcgggt cggccgcgac ggcacgcaac tcgactggcg
     1441 cttgccggaa gagcctcgcc gctgacgagg cggggcacgg cagccgcgcc ggctctactt
     1501 cgaggtcggc atcgtgaact cggcgccctt gggcgtcgag gccggccagc gctgcatcac
     1561 gctcttttgc cgggtgtaga agcgcactcc ctcctcgccg taggcgtggg tatcgccgaa
     1621 catgctcgac ttccagccgc cgaagccgtg ccaggccatc ggcaccggta tcggcacgtt
     1681 gatgccgacc atcccgatct cgacccggcg cgcgaactcg cgggcggcgt tgccgtcgct
     1741 ggtgaagcag gcgacgccgt tgccgaactc gtgctcgttg atcagcttga gcgcggcctc
     1801 gaagtcggga acgcgcacgc aggagagcac cggcccgaag atctcctccc ggtagatctt
     1861 catctgcggc gtcacgtggt cgaacagcgt gccgccggtg aagaagccct gctcgtggcc
     1921 ggcgaccgca tggccgcggc cgtcggccgc gagcgtcgcg ccgtcagcca cgcccgaggc
     1981 gatgtacgac tcgacccgct gcagcgcttc gcgggtgacc agcggcccca tctccgcgtc
     2041 ggtctccatg ccgttcttca ccaccagctt ggtcgcccgc tcggcgagca gcggcacgat
     2101 ccggtcggcg acgtcgccga ccagcaccgc caccgagatc gccatgcagc gctcgccggc
     2161 cgagccgtag gcggcaccga tcagcgcgtc gaccgcctgc tcgagattgg cgtcgggcat
     2221 caccaccatg tggttcttgg cgccgccgag cgcctgcacg cgcttgccgt ggcgcgcgcc
     2281 cgtctcgtag atgtagcgcg cgatcggcgt cgagccgacg aagctcagcg ccttgacgtc
     2341 gggatggacg agcagcgcct cgaccgcgac ccggtcgcgc tgaacgacgt tgaacacgcc
     2401 cttggggagg cccgcctcct gcagcagctc ggccatgaag atcgagggcg agggatcgcg
     2461 ctcgctcggc ttcaggatga agct
//
LOCUS       DASRDG010000295         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1650663, whole genome shotgun sequence.
ACCESSION   DASRDG010000295 DASRDG010000000
VERSION     DASRDG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650663"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFA35_04905"
     CDS             <1..686
                     /locus_tag="VFA35_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HZT55543.1"
                     /translation="TIAGHHQLLRYATQKALIDTPRTTVVACLLQGNAAYWAHCGDSR
                     LYLVRGDKLIARTRDHSYSELQQTMSQVVPMPDRFNRNVLFTCLGSPGKPVVDTVGPL
                     LLQAGDRLLLCSDGLWGTVDDATIADQLARNVISDAVPELVEQALRNGGPKSDNVSVL
                     AIEWESAEDSDSTTGISTQQLGEEVFASTIQASVLGSDAPDELDDAEIERSIREINEA
                     IRRSSQKKG"
     gene            795..1520
                     /gene="rph"
                     /locus_tag="VFA35_04910"
     CDS             795..1520
                     /gene="rph"
                     /locus_tag="VFA35_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZT55544.1"
                     /translation="MTFERTDNRPAASLRPVTITRRYTKHAEGSVLIEFGATKVLCTA
                     SVEEKVPPHKRGSGEGWVTAEYGMLPRATHTRSDREAARGKQSGRTQEIQRLIGRSLR
                     CVFDLSRLGERTISLDCDVIQADGGTRTAAITGAFVAAHDAVGWLQAQGKLSASPIRD
                     FVAAISVGIVEGTPLLDLEYVEDAACDTDMNVVMTGAGGFVEVQGTAEGAAFSRDEMG
                     TLLGLADKGIRELVAAQRKALGL"
     gene            1523..>2103
                     /gene="rdgB"
                     /locus_tag="VFA35_04915"
     CDS             1523..>2103
                     /gene="rdgB"
                     /locus_tag="VFA35_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZT55545.1"
                     /translation="MRLVLASNNAKKLLELQALLAPAGVELVAQGSLGVSEADEPHHT
                     FIENALAKARHAAKHVNGAAIADDSGLCVDALGGAPGVYSAHFAEVDARAADREGHRA
                     IQDAANNALLLERLHGITDRRARFISTLVAVRSADDPEPLVAVGRWQGEILESPRGAG
                     GFGYDPLMFIPALSKTIAELPADVKNAHSHRAIA"
BASE COUNT          349 a          744 c          730 g          280 t
ORIGIN      
        1 ccacgatcgc tggccaccat cagctgctgc gctacgcgac gcagaaggcg ctgatcgaca
       61 cgccgcgcac gaccgtggtc gcctgcctgc tgcaaggcaa cgcggcgtac tgggcgcact
      121 gcggcgactc gcgcctgtac ctggtgcgcg gcgacaagct gatcgcccgc acccgcgacc
      181 attcctattc cgaattgcag cagacgatga gccaggtcgt gccgatgccc gaccgcttca
      241 accgcaacgt gctgttcacc tgcctgggca gcccgggcaa gccggtggtc gacaccgtcg
      301 gcccgctgtt gctgcaggcc ggcgaccgcc tgctgctgtg ctccgatggc ctgtggggca
      361 cggtcgacga cgcgaccatc gccgaccagc tcgcgcgcaa cgtgatctcg gacgccgtgc
      421 ccgagctcgt cgagcaggcg ctgcgcaacg gcgggccgaa gagcgacaac gtctcggtgc
      481 tcgcgatcga gtgggagtcg gccgaagaca gcgacagcac gaccggcatc tcgacccagc
      541 agctcggcga agaggtgttc gcgtcgacga tccaggccag cgtgctcggc agcgatgcgc
      601 ccgacgaact cgacgacgcg gagatcgaac gctcgatccg cgaaatcaac gaggcgatcc
      661 ggcgttcgtc gcagaagaag ggctgatcgc caaagcgtgg accgcgggcc gagcgccggg
      721 ccgccccaag ccggcccgca ccccctcggg gggtgaccgc cgtactcggc ggtcgggggc
      781 gccacaatac gcggatgaca ttcgaacgca ctgacaaccg ccccgcggcc tcgctccgcc
      841 ccgtcaccat cacccgccgc tacaccaagc acgccgaagg ctcggtgctg atcgagttcg
      901 gcgcgaccaa ggtgctgtgc accgcgtcgg tcgaagagaa ggtgccgccg cacaagcgcg
      961 gatcgggcga aggctgggtc acggccgaat acggcatgct gccgcgcgcc acccataccc
     1021 gcagcgaccg cgaggccgcg cgcggcaagc agagcgggcg cacgcaggag atccagcgcc
     1081 tgatcggccg ttcgctgcgc tgcgtgttcg acctctcgcg gctcggcgaa cgcacgattt
     1141 cgctcgactg cgacgtgatc caggccgacg gcggcacgcg caccgcggcg atcaccggcg
     1201 cgttcgtcgc cgcgcacgac gcggtcggct ggctgcaggc gcagggcaag ttgagcgcgt
     1261 cgccgatccg cgatttcgtc gcggcgatct cggtcggcat cgtcgagggc acgccgctgc
     1321 tcgacctcga atacgtcgaa gatgcggcct gcgacaccga catgaacgtc gtgatgaccg
     1381 gcgccggcgg cttcgtcgag gtgcagggca cggccgaagg cgcggccttc tcgcgcgacg
     1441 agatgggcac gctgctcggc ctggccgaca agggcatccg cgagctggtc gcggcgcagc
     1501 gcaaggcact cggactctga cgatgcggct ggtgctggcg tcgaacaacg cgaagaagct
     1561 gctcgagttg caggcgctgc tcgcgcccgc cggggtcgag ttggtggcgc agggcagcct
     1621 cggcgtcagc gaggccgacg agccgcacca caccttcatc gagaacgcgc tggcgaaggc
     1681 gcgccatgcg gcgaagcatg tgaacggcgc cgcgatcgcc gacgattcgg gcctgtgcgt
     1741 cgacgcgctc ggcggcgcgc cgggcgtcta ttcggcgcac ttcgccgagg tcgacgcgcg
     1801 cgctgccgat cgcgaagggc atcgcgcgat acaggacgct gccaacaacg cgctgttgct
     1861 cgaacgcctg catggcatca ccgaccgccg cgcgcgcttc atcagcacgc tggtggccgt
     1921 aaggtcggcc gacgaccccg agccgctggt tgccgtcggc cgctggcagg gcgagattct
     1981 cgaatcgccg cgcggcgctg gcggcttcgg ctacgacccg ctgatgttca tcccggcact
     2041 cagcaagacc atcgccgagc tgccagcgga cgtgaagaac gcgcacagcc accgtgcgat
     2101 cgc
//
LOCUS       DASRDG010000296         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_46293, whole genome shotgun sequence.
ACCESSION   DASRDG010000296 DASRDG010000000
VERSION     DASRDG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46293"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1400)
                     /locus_tag="VFA35_04920"
     CDS             complement(135..1400)
                     /locus_tag="VFA35_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT55546.1"
                     /translation="MQTRKLIGALAATTLLIGLGGEAAAQTKLRVFAGGSGQRPDLMR
                     KLFDQYQAKNPGVTVEMETGGATSELQRQYLSTVLNAKDPAIDVYMIDIVNPAQYYGA
                     GWLEPLDAYVGAPAVALKSYLPVYATADVVDGKVAALPAFADAQFMYYRKDLLEKYKL
                     AEPKTWDELTAAAKKIQGGEGNANLQGLSIQGAPIEGAVCTFLIPYWSQGKEFNDAAG
                     KMTLDKAAAAKGLSQWLGMVDAGVIKKNVAEVKTPDTVNEFKAGQVVFAVNWGFAWDR
                     FQTDKDSQVQGKVGVMPLPAMTGGKSATCVGGWQWALSAFSKHKAEGAKLVKFLASPE
                     ASKLLAIEGSLLPTSQAVYTDAAVVKAVPWFKDAGNVVVAGRSRPMSRDYGQVSDAIR
                     TTTSAVLARTKKPDEGVAEIEGRLRRVMR"
     gene            complement(1436..>2399)
                     /locus_tag="VFA35_04925"
     CDS             complement(1436..>2399)
                     /locus_tag="VFA35_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT55547.1"
                     /translation="VGPSGCGKSTLLRMIAGLETISGGELRIGGDLVNDKPPRARDIA
                     MVFQDYALYPHKSLYDNMAFGLRLRKTPEDEIKKRVMDAARLLRIDHMLERRPAALSG
                     GQRQRVAIGRAIVRQPKVFLFDEPLSNLDAQLRQEMRSEIKRLHERLGATMIYVTHDQ
                     VEAMTLADRIAVLSAGHKMQYDTPDAIYNRPAAVFVAGFTGAPPMNLMDANVSAGSAD
                     LGEGVRMALPAALAARNGACKLGMRPENLRLAPEAGDIAVPAHVSLLEPLGSETLVSL
                     KLGAAEVIARFAANFREPPGTPVQLHLNPAHLHLFDSKSGVALV"
BASE COUNT          332 a          812 c          822 g          433 t
ORIGIN      
        1 cgcccgatcg ggtagaccac gatcagcacg agcagcacga acgcgggggc gagcagcatc
       61 gcggcgagcg tcttctcgcc ggggtctttg aagcggtcga gtttcatggt gtcggccctg
      121 tgtgcggcac gcgctcagcg catcacgcgg cgcaggcggc cctcgatctc ggcgacgcct
      181 tcgtcgggct tcttggtgcg tgcgagcacc gcgctggtcg tcgtgcggat cgcatcgctg
      241 acctgcccgt agtcgcgcga catcggacgg ctgcggccgg cgacgacgac gttgcccgcg
      301 tccttgaacc agggcacggc cttgacgacg gccgcgtcgg tgtagacggc ttgcgaggtc
      361 ggcaacagcg atccttcgat cgcgaggagc ttgctcgcct cggggctggc caggaacttc
      421 accagcttcg cgccttcggc cttgtgcttc gagaacgcgc tcaacgccca ttgccagccg
      481 ccgacgcagg tcgcgctctt gccgccggtc atcgcgggca gcggcatcac gccgaccttg
      541 ccctgcacct ggctgtcctt gtcggtctgg aagcgatccc acgcaaagcc ccagttgacg
      601 gcgaacacga cctggccggc cttgaactcg ttgaccgtgt cgggcgtctt cacctcggcg
      661 acgttcttct tgatcacgcc cgcatcgacc atgccgagcc actgcgagag gcccttggcc
      721 gcggcggcct tgtcgagcgt catcttgccg gcggcgtcgt tgaactcctt gccctgactc
      781 cagtacggaa tcaggaaggt gcagaccgcg ccttcgatcg gcgcgccttg aatggacagg
      841 ccttgcaggt tcgcgttgcc ttcgccgccc tggatcttct tcgcagccgc ggtgagctcg
      901 tcccaggtct tgggctcggc gagcttgtac ttctcgagca ggtccttgcg gtagtacatg
      961 aactgcgcgt cggcgaaggc cggcagcgcc gcgaccttgc cgtcgacgac gtcggcggtc
     1021 gcgtagaccg gcaggtacga cttcagcgcg acagccggcg cgccgacgta ggcgtccagc
     1081 ggctcgagcc agccggcgcc gtagtactgc gcggggttga cgatgtcgat catgtagacg
     1141 tcgatcgccg ggtccttcgc gttcagcacc gtgctcaggt actggcgctg cagctccgag
     1201 gtcgcgccgc cggtctccat ctcgacggtg acgccggggt tcttcgcctg gtactggtcg
     1261 aagagcttgc gcatcaggtc cgggcgctgg cccgagccgc cggcgaagac gcgcagcttg
     1321 gtttgcgccg cggcctcgcc gccgaggccg atcagcagtg tcgtcgcggc gagggcgccg
     1381 atcagtttgc gtgtttgcat gaatgtctcc taaggggtgg gttggcggtg gcccttcaga
     1441 cgagggcgac tccgctcttc gaatcgaaca ggtgcaggtg cgccggattc agatgcaact
     1501 ggaccggcgt gcccggcggc tcgcgaaagt tcgccgcgaa gcgcgcgatg acttcggccg
     1561 cgccgagctt caggctcacc agcgtctccg agccgagcgg ctccagcagc gacacatgtg
     1621 ccggcacggc gatgtcgccg gcctccggcg cgagtcgcag gttctcgggc cgcatgccga
     1681 gcttgcacgc gccgttgcgc gcggcgagcg ccgccggcag cgccatgcgc acgccctccc
     1741 ccagatcggc cgaccccgca ctgacgttcg cgtccatcag gttcatcggc ggcgcgccgg
     1801 tgaagccggc gacgaagacc gccgcgggcc ggttgtagat cgcgtcgggc gtgtcgtact
     1861 gcatcttgtg gccggcgctc agcaccgcga tgcgatcggc cagggtcatc gcctcgacct
     1921 ggtcgtgcgt cacgtagatc atcgtcgcgc cgaggcgctc gtgcagtcgc ttgatctcgc
     1981 tgcgcatctc ctggcgcagc tgggcgtcga gattcgacag cggttcgtcg aacaagaaga
     2041 ccttgggttg ccgcacgatc gcgcggccga tcgcgacgcg ttgtcgctgg ccgccggaga
     2101 gtgccgccgg gcgacgctcg agcatgtggt cgatgcgcag caggcgcgcc gcgtccatca
     2161 cgcgcttctt gatctcgtct tccggcgtct tgcgcaggcg caggccgaac gccatgttgt
     2221 cgtacaggct cttgtgcgga taaagtgcgt agtcctggaa caccatcgcg atgtcgcgtg
     2281 cacgcggcgg cttgtcgttg acgaggtcgc cgccgattcg cagctcgccg ccgctgatcg
     2341 tctccaggcc ggcgatcatg cgcagcaagg tgctcttgcc gcagcccgac gggccgacg
//
LOCUS       DASRDG010000297         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_500203, whole genome shotgun sequence.
ACCESSION   DASRDG010000297 DASRDG010000000
VERSION     DASRDG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500203"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VFA35_04930"
     CDS             <1..887
                     /locus_tag="VFA35_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZT55548.1"
                     /translation="TAFGEKMVMRIFDPDTTVKPLEGLGFGSHDGKRWEQLVANPHGI
                     ILVTGPTGSGKTTTLYSTLRRLATDEVNVCTIEDPIEMIQPALNQTQVQPAIDLNFAE
                     GLRALMRQDPDIIMVGEIRDLDTAQMAVQASLTGHLVLSTLHTNDAPSAITRLLDLGV
                     PHYLIASTLNGVLAQRLVRTLCPNCKQPDDGLTREKLDEVVKPWRLSGAIKPCKPVGC
                     LECRMTGYRGRAGLYELLTVTDAARGAIHPVTDAAKLRHQSLADGLRPLRLAGAMKVA
                     EGLTTLDEVLRATPAWES"
     gene            933..1397
                     /locus_tag="VFA35_04935"
     CDS             933..1397
                     /locus_tag="VFA35_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZT55549.1"
                     /translation="MKIKSQRDFFSGLMFIVVGVIFAVGATNYSMGSSAKPGAGYFPL
                     ILSVLMAILGAIVLFKSLTIETEGGDAIGDIAWRPLLVIVASIAVFGLALPRLGMFMT
                     IPLLIVMVSFAGDEFSWKGVIANSLVLTLGSWVIFVWGLNLTIPLKPGFMGG"
     gene            1399..>2218
                     /locus_tag="VFA35_04940"
     CDS             1399..>2218
                     /locus_tag="VFA35_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZT55550.1"
                     /translation="MDLLSNLALGFGVAFTVQNLLYAFFGCVLGTLIGVLPGLGPVAT
                     IAMLLPSIYALDATPALIMLAGIYYGAQYGGSTTAILINVPGESSSVVTAIDGYAMAR
                     KGRAGSALAAAGLGSFFAGCVGTVIIAAFAPPLTELAFKFGPAEYFSLMVLGLIGAVV
                     LASGSLVKAIAMIILGLLLAQINTDVISGTARYSFDIPELTDGIGFVVIAMGVFGFGE
                     IIANLGQPAEHREVFTKEVKGLWPTKRDFMEAWPSVLRGTALGCILGVLPGGGAL"
BASE COUNT          328 a          763 c          745 g          382 t
ORIGIN      
        1 cgaccgcgtt cggcgagaag atggtgatgc gcatcttcga ccccgacacg accgtcaagc
       61 cgctcgaagg actcggattc ggtagccacg acggcaagcg ctgggagcag ctcgtcgcca
      121 acccgcacgg catcatcctc gtgaccggtc cgaccggctc gggcaagacg acgacgctgt
      181 actcgacgct gcgccgcctg gcgaccgacg aggtcaacgt ctgcacgatc gaggacccga
      241 tcgagatgat ccagccggcg ctgaaccaga cccaggtgca gccggcgatc gacctcaact
      301 tcgcggaggg cctgcgcgcg ctgatgcgcc aggacccgga catcatcatg gtcggcgaga
      361 tccgcgacct cgacaccgcg cagatggcgg tccaggcctc gctgaccggc cacctcgtgc
      421 tgtcgaccct gcataccaac gacgcgccct cggcgatcac ccggctgctc gacctcggcg
      481 tgccgcacta cctgatcgcc tcgaccctca acggcgtgct cgcgcagcgc ctggtgcgca
      541 cgctgtgccc gaactgcaag cagcccgacg acgggctcac gcgcgagaag ctcgacgagg
      601 tcgtcaaacc ctggcgcctg agcggcgcga tcaagccgtg caaaccggtc ggctgcctcg
      661 aatgccgcat gaccggctac cgcggccgtg ccgggctgta cgaactgctg accgtgaccg
      721 acgcggcgcg gggcgcgatc cacccggtca ccgacgcggc caagctgcgc caccagtcgc
      781 tcgccgatgg cctgcgcccg ctgcggctgg ccggcgcgat gaaggtggcc gagggcctga
      841 ccacgctcga cgaggtcttg cgcgccacgc cggcgtggga gagctgacgc gcgctcctag
      901 aatccgccgc aacgcagagt cggagttcct tcgtgaagat caagagccag cgagatttct
      961 tttccggatt gatgttcatc gtcgtcggcg tgatcttcgc cgtcggcgcc accaactatt
     1021 cgatgggcag ctcggccaag ccgggggccg gctacttccc gctgatcctg tcggtgctga
     1081 tggcgatcct cggcgcgatc gtgctgttca agtcgctgac gatcgaaacc gagggcggcg
     1141 acgcgatcgg cgacatcgcc tggcggccgc tgctggtgat cgtcgcgtcg atcgcggtgt
     1201 tcggcctggc gttgccgcgc ctgggcatgt tcatgacgat cccgctgctg atcgtgatgg
     1261 tcagcttcgc gggtgacgag ttcagctgga aaggcgtgat cgccaacagc ctggtgttga
     1321 cgctgggttc ctgggtcatc ttcgtttggg gcctgaacct gacgattccg ctgaagcccg
     1381 gcttcatggg cggctgagat ggacctgctc agcaacctcg cgctcggctt cggcgttgcg
     1441 ttcaccgttc agaacctgct ttacgcgttt ttcggctgcg tgctcggcac gctgatcggc
     1501 gtgctgcccg ggctcggccc ggtcgcgacg atcgcgatgc tgctgccctc gatctacgcg
     1561 ctcgacgcga cgccggcgct gatcatgctc gccggcatct actacggcgc ccagtacggc
     1621 ggctcgacca cggcgatcct gatcaacgtg ccgggcgagt cgagctcggt cgtgaccgcg
     1681 atcgacggct acgcgatggc gcgcaagggc cgcgccggct cggcgctcgc cgccgccggg
     1741 ctgggctcgt tcttcgccgg gtgcgtcggc accgtcatca tcgcggcgtt cgcgccgccg
     1801 ctgaccgaac tcgcgttcaa gttcgggccg gccgagtact tctcgctgat ggtgctgggc
     1861 ctgatcggcg cggtggtgct ggcctcgggg tcgctcgtga aggcgatcgc gatgatcatc
     1921 ctgggcctgc tgctggcgca gatcaacacc gacgtgatct cgggcaccgc gcgctacagc
     1981 ttcgacatcc cggagctgac cgacggcatc ggcttcgtcg tgatcgcgat gggcgtgttc
     2041 ggcttcggcg agatcatcgc caacctgggc cagccggcag agcatcgcga ggtcttcacc
     2101 aaggaggtca agggcctctg gccgaccaag cgggacttca tggaggcctg gccgtcggtc
     2161 ctgcgcggca ccgcgctcgg ctgcatcctc ggcgtcctgc ccggcggcgg cgccttgc
//
LOCUS       DASRDG010000298         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_46430, whole genome shotgun sequence.
ACCESSION   DASRDG010000298 DASRDG010000000
VERSION     DASRDG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46430"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..>2053)
                     /gene="recG"
                     /locus_tag="VFA35_04945"
     CDS             complement(180..>2053)
                     /gene="recG"
                     /locus_tag="VFA35_04945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZT55551.1"
                     /translation="EKGFRFRPQLRIAIADESQHTLLLRFFHFNNAQVNQLAVGTRLR
                     CYGEVRRGQHSLEMVHPQYQRLAEDAASEVEERLTPIYPTTEGLGQKRLGGLIQRALA
                     LLPQDELLELIPPALRETNAAISLRDALLYMHRPPPDADLVQLGLGTHPAQRRLAFEE
                     LLAQHLSLRQLRNRLRAHAAPALRGDGKLRKKFLGALPFAPTKAQARVAAEVARDIGE
                     TAPMLRLVQGDVGSGKTVVAALAALAAVEAGRQAALMAPTELLAEQHFINFHKWISPL
                     GIEVVWLAGKLKGKARNAALTSLRGAAQIAIGTHALMQEGVEFDRLGLVIIDEQHRFG
                     VDQRLALRDKGRAGDEIPHQLVLTATPIPRTLAMTAYADLDVSIIDELPPGRTPVATV
                     VLNGTRRSEVVERIRAACAEGRQAYWVCTVIEESEVLEAQAAEVAHAELAAALPDMRI
                     GLVHGRMKPVEKQAAMEAFKRGEISLLVATTVIEVGVDVPNASLMVIENAERLGLAQL
                     HQLRGRVGRGSAKSSCVLLYQAPLSATARARLDVLRETNDGFRIAEKDLELRGPGEVL
                     GTRQTGEAAFRVADLARDAHLLPAVQRAGEILMREHPDLVARLIARWIGQAARFAGA"
BASE COUNT          332 a          707 c          697 g          317 t
ORIGIN      
        1 caggacgtcc acctgcgcaa cggcctcaac gtcgccgacg gcaaggtcac ctgcgaaccg
       61 gtcgccgaag cgcacggcct gccctacgtc aaagcggaaa ccctgatcgg cctgtaatcg
      121 cctcgatcac agcgacccgc ctcgcttgac cggaggcggg ccgactgcgc aagactcgcc
      181 taggcgccgg cgaagcgcgc ggcctgaccg atccaacgcg cgatcaggcg cgcgaccaaa
      241 tcagggtgct cgcgcatgag gatttcgccc gcgcgctgta ccgcgggcag caggtgcgcg
      301 tcgcgcgcga gatcggcgac gcggaatgcg gcctcaccgg tctggcgcgt gccgagcact
      361 tcgccaggac cgcgcaactc cagatccttc tcggcgatgc ggaagccgtc gttggtctcg
      421 cgcagcacgt ccaggcgcgc gcgcgccgta gccgacagcg gcgcctggta taaaagtaca
      481 cagctggatt tcgcactgcc gcggccgacg cggccgcgca gctggtgcaa ctgggcgagg
      541 ccgaggcgtt cggcgttctc gatcaccatc agactcgcgt tcggcacgtc gacgccgacc
      601 tcgatcaccg tcgtcgcgac gagcagcgaa atctcgccgc gcttgaacgc ctccatcgcc
      661 gcctgcttct cgaccggctt catgcgccca tgcacgaggc cgatgcgcat gtccggcaac
      721 gccgcggcca gttcggcgtg ggccacctcg gccgcctgcg cttcgagaac ttcggattcc
      781 tcgatcaccg tgcagaccca gtacgcctgc ctgccctcgg cacacgcggc acgaatgcgc
      841 tcgacgacct cgctgcgacg cgtgccgttg agcacgacgg ttgccacggg cgtgcgtccg
      901 ggcggcagct cgtcgatgat cgagacgtcg agatcggcat acgcggtcat cgccagcgtg
      961 cgcgggatcg gcgtcgcggt cagcacgagc tggtgcggaa tctcgtcgcc ggcgcgaccc
     1021 ttgtccctca gcgccaggcg ttgatcgacg ccgaagcgat gctgctcgtc gatgatgacg
     1081 aggccgagac ggtcaaactc gacgccctcc tgcatcagcg catgcgtgcc gatcgcgatc
     1141 tgcgcggcgc cgcgcaggct ggtcagcgcg gcgttgcgtg ccttgccctt gagtttgccg
     1201 gccagccaga cgacctcgat accgagtgga gaaatccact tgtggaaatt gataaaatgc
     1261 tgctcggcga gcagttcggt cggcgccatc aacgcggcct ggcggccggc ctcgaccgcg
     1321 gcgagcgcgg ccagcgcggc gacgacggtc ttgcccgagc caacgtcacc ctggacaagg
     1381 cgcagcatcg gcgcggtttc gccgatatcg cgcgccactt ccgcggcgac acgcgcctgc
     1441 gccttggtcg gcgcgaacgg cagcgcacca aggaattttt tcctcagctt gccgtcgccg
     1501 cgcagcgcgg gtgccgcgtg cgcgcgcagg cgattgcgca actggcgcag cgaaagatgc
     1561 tgggcgagca gttcctcgaa cgcgagccgg cgctgcgccg ggtgcgtgcc gaggccaagt
     1621 tgcacgagat ccgcgtcggg cggcggccga tgcatgtaga gcagcgcatc gcgcagcgag
     1681 atcgctgcgt tcgtctcgcg cagcgcggga ggaatcagtt ccagcaactc gtcctgcggc
     1741 aacagcgcga gcgcacgctg gatcaggccg ccgaggcgct tctggccgag gccttcggtg
     1801 gtcggataga tcggcgtcag gcgctcctcg acttcgctcg cggcatcctc ggccaggcgc
     1861 tggtactgcg gatgcaccat ctcgaggctg tgctggccgc gacgcacctc gccatagcag
     1921 cgcaggcgcg taccgacggc gagctggttg acctgtgcgt tgttgaagtg gaagaagcgc
     1981 agcagcaagg tgtgctggct ctcgtcggcg atcgcgatgc gcagttgcgg acggaagcgg
     2041 aaacccttct cga
//
LOCUS       DASRDG010000299         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_849772, whole genome shotgun sequence.
ACCESSION   DASRDG010000299 DASRDG010000000
VERSION     DASRDG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849772"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..430)
                     /locus_tag="VFA35_04950"
     CDS             complement(56..430)
                     /locus_tag="VFA35_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55552.1"
                     /translation="MAASACGAVAVAGTPPHFATQTITVTAAGLRPGGEVRIPAFGTV
                     VFKNELAEGDVVVRIARPFEPSENCSTSLGFTGAGVESASKPIPPRGVAALCFHDAGA
                     FPFTVQAPGGEWRGTIAVGGAP"
     gene            complement(537..>2475)
                     /locus_tag="VFA35_04955"
     CDS             complement(537..>2475)
                     /locus_tag="VFA35_04955"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZT55553.1"
                     /translation="PGRSRAAARPAGAVVNLLSVNWLWTNTAVALLLALLVAAGCRIA
                     RPRPAIAHALWLLVLLRLCLPPWLAVPLPIVWRGAGAAAPQVVDLSSDAVRLAAPPAP
                     VVAPIVAIGAVPFTSTGTSTGTSAGTWLWFGGSSAMALLALASMWRGFRRYGAAPQPA
                     VWVAAEVRTLAAQLGVRAPALADDPSASTPFVWPFGRPLLVVSTHALAAMAPPARMAV
                     LAHELGHLARRDHWWLHGELLCAIAFFWHPLFWLLRARVREQAELACDAWALAAAPAA
                     RRSYASALIDALAREHPAPAVPVLAARPAARRLIERRLSMILHESVPCRLPRLALAGV
                     ASLALCLAVAPVRAQGRGESPRVEIRVDGRRLEDLSDAERERVLQRLGVTGEALRLVQ
                     KARALEHTGTSADEPQEPKPKPRPKPKANRPAADVDLQDTTAGVQDQVRAALDEARRE
                     IRSDADLRELGITKQVEGLIDSLGGRGDFQQSLGEVIAGAMKGAAKMAKREVQQDPDL
                     KKLGIADSLGSLIDGLAGDRRLQEGLQQIVQQALKSAAVAAKVEIAEDGDLKKLGITA
                     DVSALLDDLGNGKLDLGGHLPKLIEKAMQGALQGAKFEVRIGDDEPDGEQPVEKRKEK
                     AKDDKAKDRPKAKDQPKRRVF"
BASE COUNT          330 a          962 c          878 g          305 t
ORIGIN      
        1 cccgcgcagc ccgcgagcgc gagcagcgcg ccgccgcagc agcaccgcgc ggcgctcacg
       61 gcgccccgcc cacggcgatc gtgccgcgcc actcgccgcc cggcgcctgc accgtgaagg
      121 ggaacgcgcc cgcgtcgtgg aagcacagcg ccgccacgcc ccgaggcggg atcggcttcg
      181 acgccgactc cacgcctgcc ccggtgaagc cgagcgacgt cgagcagttc tcggacggct
      241 cgaacggccg cgcgatgcgt acgacgacgt cgccttccgc gagttcgttc ttgaacacca
      301 cggtgccgaa cgcggggatg cgcacctcgc cgccgggccg caggcccgcc gcggtcacgg
      361 tgatcgtctg cgtcgcgaag tgcggcggcg tgccggcgac cgccacagcg ccgcaagcgc
      421 tcgccgccac ggcgagggga aggcaacagg cgaccgccgc gcgccgccac aggtcgggac
      481 ggaccatggc agaaggatgc cgccgggcag ctccggttgc acgcgccgcg gcggcgtcag
      541 aagacgcggc gcttcggctg gtccttcgcc ttgggcctgt ccttggcctt gtcgtccttg
      601 gccttctcct tgcgcttctc caccggctgc tccccgtccg gttcgtcgtc gccgatgcgc
      661 acctcgaact tcgccccctg cagcgcgccc tgcatcgcct tctcgatcag cttcggcagg
      721 tgcccgccga gatcgagctt gccgttgccg aggtcgtcga gcagcgccga cacgtcggcg
      781 gtgatgccga gcttcttcag gtcgccgtcc tcggcgatct cgaccttcgc ggcgacggcc
      841 gcgctcttca gcgcctgctg cacgatctgc tgcaggccct cctgcaggcg gcggtccccg
      901 gcgagaccgt cgatcagcga accgagcgaa tcggcgatgc cgagcttctt caggtccggg
      961 tcctgctgca cctcgcgctt tgccatcttc gcggcgccct tcatcgcgcc cgcgatgacc
     1021 tcgccgagcg actgctggaa gtcgcccctg ccgcccagcg agtcgatcag gccttcgacc
     1081 tgcttcgtga tgccgagttc gcgcagatcc gcgtcgctcc ggatctcgcg cctcgcctcg
     1141 tcgagcgccg cgcgcacctg atcctgcact ccggccgtcg tgtcctgcag gtcgacgtcc
     1201 gccgccggcc gattcgcctt cggcttcggc ctgggcttcg gtttcggctc ctgcggctcg
     1261 tcggcggatg tgccggtgtg ctcgagggcg cgcgccttct gcaccaggcg cagcgcctcg
     1321 ccggtcacgc cgagccgctg cagcacgcgt tcgcgctcgg cgtcgctcag gtcctcgagc
     1381 cgtctgccgt cgacgcggat ctcgacgcgg ggcgactcgc cgcgcccctg cgcgcgcacg
     1441 ggcgcgacgg cgaggcacag ggcgagcgaa gcaacgccgg cgagcgcgag acgcggcagc
     1501 cggcagggaa ccgattcgtg caggatcatc gacaacctcc gttcgatcag gcgccgggcc
     1561 gccggcctcg ctgcgaggac cgggaccgcg ggcgcgggat gctcgcgcgc gagcgcgtcg
     1621 atcaacgcgc tcgcgtacga ccggcgcgcc gcaggggcgg cggcgagcgc ccaggcatcg
     1681 cacgcgagtt cggcctgttc gcgcacgcgc gcgcgcagga gccagaacag cggatgccag
     1741 aagaaggcga tcgcgcacag cagttcgccg tgcagccacc agtggtcgcg ccgcgccagg
     1801 tggccgagct cgtgcgcgag cacggccatg cgcgccggcg gcgccatcgc cgcgagcgca
     1861 tgcgtgctga cgaccagcag cggccggccg aacggccaca cgaacggcgt gctcgccgaa
     1921 ggatcgtcgg cgagcgccgg cgcgcggacc cccagctgcg cggcgagcgt ccgcacctcg
     1981 gccgccaccc agaccgccgg ctgcggcgcc gccccgtagc ggcggaaccc gcgccacatg
     2041 ctcgcgagcg cgagcagggc catggcgctg ctccccccga accagagcca ggtcccagcc
     2101 gaggtcccag tcgaggtccc agtcgaggtg aacggaaccg ccccgatggc gacgatcggc
     2161 gcgacgaccg gtgccggcgg cgccgcgagc cgcacggcgt ccgacgacag atcgacgacc
     2221 tgcggggccg ccgccccggc gccgcgccag acgatcggca gcggcacggc gagccacggc
     2281 ggcaggcaga gccgcagcag cacgagcagc cacagcgcgt gcgcgatcgc cggccgcggc
     2341 cgcgcgatgc ggcagccggc cgcgaccagc agcgcgagca gcagcgccac cgcggtgttg
     2401 gtccagagcc agttcacaga gaggaggttc acgacggccc ccgccggccg cgcggcggcg
     2461 cgcgatcgtc caggt
//
LOCUS       DASRDG010000300         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1428231, whole genome shotgun sequence.
ACCESSION   DASRDG010000300 DASRDG010000000
VERSION     DASRDG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428231"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFA35_04960"
     CDS             <1..392
                     /locus_tag="VFA35_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT55554.1"
                     /translation="LSDRYDSRVLLCVYYGLRGLSLLYLPWSDFSIGSLGLFTVFYGL
                     DWIATVPPTVRLTTDRFGPQLAPLVYGWIGAGHQLGAATAAYGAGVLRVQTNHYVEAF
                     LVAGAACLVAAVMALSIRRNTMALPAH"
     gene            405..1232
                     /locus_tag="VFA35_04965"
     CDS             405..1232
                     /locus_tag="VFA35_04965"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZT55555.1"
                     /translation="MPDPALQQQLIHQLQHAANYVLFGGFVLALAGEMIFPRFADDGP
                     QRRVAHGLHNLLLWIAGIVVMSLAFGSSAWFALNWLQAHGVGVLAMLHLTGALHAVLA
                     FLLLDLCDYFFHRLSHNVRWLWLLHAVHHSDPAVDVTTNLRQHPAHILLTQLWKLLVA
                     AAVGVPMWVFLLHETLAIGMAHLHHAAIRWPRWIDKAFAWLVITPRMHWNHHSPEAAL
                     TNSNYAVVLSIWDRSFRTFTPATADSRFGLDSLRDTRWHSTWGMLATPWRARKLPRL"
     gene            complement(1264..1830)
                     /locus_tag="VFA35_04970"
     CDS             complement(1264..1830)
                     /locus_tag="VFA35_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55556.1"
                     /translation="MFGKSLLRLFSILVALAFCVPSWAGVPILVTVTSPDDATNFNSS
                     SVGTPVSATITFTGHSNTPGQVAQLSGIGLSGTNAADFAITGGTCANGTNLADGNTCT
                     VTIRYLPSSVPKETAQLNLNCTIVAVLGGFSLSCSNAGASGSYALYGFAAAALTAVSA
                     PLLDPKLLTALSAMLLGLGVFFAARRNA"
     gene            complement(1963..>2275)
                     /locus_tag="VFA35_04975"
     CDS             complement(1963..>2275)
                     /locus_tag="VFA35_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the deimination of
                     N-formimino-L-glutamate to ammonia and
                     N-formyl-L-glutamate; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HZT55557.1"
                     /translation="ANRSVGETLYAAALRGGARAAGAATGAIETGARADLIVLDNNSP
                     QLAGRDEASLVDTFLFAGNANLVRHVMANGDWVVRDFRHRDEERIAARYRRTLLALAD
                     R"
BASE COUNT          357 a          781 c          733 g          404 t
ORIGIN      
        1 ggctgtccga ccgctatgac agccgcgtgc tgctgtgcgt ctactacggc ctgcgcggac
       61 tctcgctgtt gtacctgccg tggtcggatt tcagcatcgg cagcctcggc ctgttcaccg
      121 tgttctacgg cctcgactgg atcgccaccg tgccgccgac cgtgcgcctg accaccgacc
      181 gtttcggtcc gcagctcgcc ccgctcgtct acggctggat cggcgccggc caccagctcg
      241 gcgccgccac cgcagcctat ggcgcgggcg tgctgcgcgt gcagaccaac cactacgtcg
      301 aggctttcct ggtcgccggt gcggcctgtc tcgtcgccgc ggtgatggcg ctgtcgatcc
      361 gccgcaatac aatggcgctg cccgcccact gacttttcct tggcatgccg gatccggccc
      421 tccaacagca gttgatccac cagctccagc acgccgcgaa ctatgtcctg ttcggcggct
      481 tcgtgctcgc gctcgccggc gagatgatct ttccgcggtt cgcggacgac ggcccgcagc
      541 gccgcgtcgc gcacggcctg cacaacctgc tgctgtggat cgccggcatc gtggtgatgt
      601 cgctcgcctt cggcagttcg gcatggttcg cgctgaactg gttgcaggcg cacggcgtcg
      661 gcgtgctcgc gatgctgcac ctgaccggtg cgctgcatgc cgtgctggcc ttcctgctgc
      721 tcgacctgtg cgactacttc ttccatcggc tcagtcacaa cgtgcgctgg ctgtggctgc
      781 tgcacgccgt gcatcactcc gatcccgccg tcgacgtgac cacgaacctg cgccagcatc
      841 ccgcgcacat tctcttgacc cagctctgga aactgctggt tgccgcggcg gtcggcgtgc
      901 cgatgtgggt gttcctgctg cacgagacgc tggcgatagg catggcgcac ctgcaccacg
      961 ccgcgatccg ctggccgcgc tggatcgaca aggcgttcgc ttggctcgtg atcacgccgc
     1021 gcatgcattg gaaccatcac tcgcccgagg cggcgttgac gaattcgaac tatgcggtcg
     1081 tgctttcgat ctgggatcgg tcgtttcgca cgttcacgcc cgcgacggcg gattcgcgct
     1141 tcggtctcga ctcattgcgc gacacgcgct ggcacagcac ctggggcatg ctcgcgacgc
     1201 cttggcgcgc gcgcaaactc ccgcggcttt gacgcggcgg gagcggcgga atccgacggc
     1261 ggatcaggcg ttgcgacgcg cggcgaagaa gacgccgagg ccgagcagca tcgccgacag
     1321 cgcggtcagc agcttcggat cgagcagcgg cgccgatacc gcagtcaggg ccgccgccgc
     1381 aaaaccgtac agcgcgtagg agccgctcgc gcccgcattg ctgcagctca acgagaaccc
     1441 gcccaatacc gcaactatcg tgcagttgag attcaactgc gcggtttcct tgggaacgga
     1501 cgaaggcagg tagcgaatgg tcacggtgca ggtgtttccg tccgcgagat tcgtgccgtt
     1561 cgcgcaggtg ccgcccgtga tcgcgaaatc ggccgcgttc gtgcctgaca gaccgattcc
     1621 gctcaactgc gcgacttgac caggcgtatt cgaatggccg gtgaacgtga tcgttgccga
     1681 taccggagtg ccgaccgagg acgagttgaa attcgtggca tcgtcgggcg aagtgacggt
     1741 caccaggatg ggcacgcccg cccacgaggg cacacaaaat gccagggcga cgagaatcga
     1801 aaaaagacgc aatagagact tgccgaacat ttccccactc ctcgttcgaa tagccgcccc
     1861 gcaatctacc acaaccatgt tcgggcggcg caaatcgccg gtcgcggcgc cggcgcggaa
     1921 ctggccgccg ggcagggtcg cgtgacggcc gcccgggacg aatcagcgat ccgcaagcgc
     1981 gagcagtgtt cggcgatagc gcgcggcgat gcgctcctcg tcgcgatggc ggaaatcgcg
     2041 cacgacccag tcgccgttcg ccatgacgtg gcgcaccaga ttcgcgttgc cggcaaacag
     2101 gaatgtgtca accagactgg cttcgtcgcg gccggcgagt tgcggcgagt tgttatcgag
     2161 cacgatcaga tccgcgcgtg cgccggtttc gatcgcgccc gtcgccgcgc cggccgcacg
     2221 cgcgccaccg cgcagcgccg cggcgtacaa ggtttcgccg acgctgcggt tcgcc
//
LOCUS       DASRDG010000301         4868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_500864, whole genome shotgun sequence.
ACCESSION   DASRDG010000301 DASRDG010000000
VERSION     DASRDG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500864"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFA35_04980"
     CDS             <1..324
                     /locus_tag="VFA35_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55558.1"
                     /translation="HRAVMHDHPNLMEWQVYACGAPIMVESAQHDFVVKCGLPDDAWT
                     GRTGLVHHAVMHDHPDLMNHQVYACGAPIMVESAHKDFVMKCGLPDDEFYADSFTSEA
                     DKHGA"
     gene            403..972
                     /locus_tag="VFA35_04985"
     CDS             403..972
                     /locus_tag="VFA35_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZT55559.1"
                     /translation="MNRQADPNTYGAVAIALHWLLALAIAGTFGVGLYMSDLPVSPLR
                     LKLYNWHKWAGVTILALSALRLVWRLAHRAPPLPERVLRAMPGWQRHAHRATHALLYG
                     LFFAVPLSGWAYSSAAGFPVVWFGVLPLPDWVAVDKDFADAVLKPLHQTCAFALAGLV
                     VLHVAATLKHQFVDRDGLFWRMWPRGTGR"
     gene            981..1523
                     /locus_tag="VFA35_04990"
     CDS             981..1523
                     /locus_tag="VFA35_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZT55560.1"
                     /translation="MALGLVAGAGHAQPVATLVPAQSEIVFTSKQMGVPVEGRFRRFD
                     VKVALDPKQPAGGSVALTIDTASATLGLPETDAELARPNWFDTAKFAQATFNSSSIKG
                     LGGGRFEVAGTLSVKGSAQPVVVPVTLTQSAGTAVATGAFTVKRLAYRIGEAEWADTS
                     LVADDVQVRFRLAFSGLAPP"
     gene            complement(1542..2363)
                     /locus_tag="VFA35_04995"
     CDS             complement(1542..2363)
                     /locus_tag="VFA35_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HZT55561.1"
                     /translation="MLSACVATMPGQPEQGASKTAVTGAAGGATAEGASGNLERCDAS
                     LGTIGVVEDQNAGWYQTLRSYKLGSTVPVLRMMIQQSNCFVVVERGAAMHNMNTERNL
                     QKSGEMRAGSNFGAGQMVAADYTMNPQITFSQNTGGVGGGLAGFSRSLGVVGALAGGL
                     KTNEASTTLIMIDNRSGVQLAAAEGSAKNTDFNVFGSTFGWAGGAGAGGYSNTPEGKI
                     IIAAFADSYNQLVRAVRNYKAQTVKGGLGTGGTLGVQGGKTSASKAVDQDAKKKK"
     gene            complement(2568..3362)
                     /locus_tag="VFA35_05000"
     CDS             complement(2568..3362)
                     /locus_tag="VFA35_05000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZT55562.1"
                     /translation="MPPTSAAPGAAPDPADSGTPDLVHLPHTPLPDYYADAPARAQFL
                     RETFDSTAPDYNRLEKLLGFGSGSWYRRTALQRAGLKPGADVLDVGMGTGLVAHEILA
                     LTNEPQRLIGVDPSPGMMAQARLPATVRCLRGTAERLPVPDASVDFLVMGYALRHITD
                     LEAAFREFHRVLRPGGTLLVLEISKPRGALANALLKAYMRGAVPLLARLVARQPATPM
                     LWRYYWDTIEACAAPEAVMRTLEGAGLREVRREVALGIFSEYLATR"
     gene            3552..3821
                     /locus_tag="VFA35_05005"
     CDS             3552..3821
                     /locus_tag="VFA35_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZT55563.1"
                     /translation="MTTAQTDLQREVAALMVEALNLDVAPESIAPDAPLYGEGLALDS
                     IDILEVALVVSQRYGFQLRSDDEDNVRIFTSLASLTEHIAAHRVS"
     gene            3829..4464
                     /locus_tag="VFA35_05010"
     CDS             3829..4464
                     /locus_tag="VFA35_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55564.1"
                     /translation="MAPRLGIRLVAIALASVAYILGCHWLMTQAHASPWNAVGVLTPM
                     LAAIAIGAWRAGQHLPAGLAALAIGGLCAQALFGVQVPPPLLYLAQHAGIHLFLAGVF
                     GSTLRAGHTPLITTLARRVHRRFTPEMAAYTRKCTIAWVGYFVAMAVASVVLYAFAAF
                     DTWALFANLVTPVTLAAMFGAEYLLRYRLHPEFERASFADAIRSYLHKAAD"
     gene            4476..>4868
                     /locus_tag="VFA35_05015"
     CDS             4476..>4868
                     /locus_tag="VFA35_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ligase"
                     /protein_id="HZT55565.1"
                     /translation="MALPLILADDLDAPLAWRDGMPVSRRQYLADVAALAARLPGAGP
                     MLNFSADRYRFAVGLGAAMRCGQASLMPPNHTPDTVARLRTLFPATYALADAGAPALQ
                     LPTLHHAAAPATAAEVAAQTAVPEIDEAA"
BASE COUNT          648 a         1782 c         1669 g          769 t
ORIGIN      
        1 caccgggccg tgatgcacga ccacccgaac ctgatggagt ggcaggtgta cgcctgcggc
       61 gcgccgatca tggtcgagtc ggcgcagcac gacttcgtcg tcaagtgcgg gctgcccgac
      121 gacgcgtgga ccggtcgcac cggcctcgtc caccacgcgg tgatgcacga ccacccggac
      181 ctgatgaacc accaggtcta tgcctgcggc gcgccgatca tggtcgagtc ggcgcacaaa
      241 gacttcgtga tgaagtgcgg actgcccgac gacgagttct acgccgactc gttcacgtcg
      301 gaggccgaca agcatggcgc gtagcggcat gtttgcggtg cagcctgact tcgcgcagcg
      361 aagtcaaggc acgacgtgcc ggccgaaacc acaacacggc gcgtgaaccg ccaggccgac
      421 cccaacacct atggcgccgt cgccatcgcg ctgcactggc tgctcgcgct cgcgatcgcc
      481 ggcaccttcg gcgtcggcct ctatatgagc gacctgcccg tctcgccgct gcgcctgaag
      541 ctgtacaact ggcacaagtg ggccggcgtg acgatcctcg cgctctccgc gctgcgcctg
      601 gtgtggcggc ttgcgcaccg cgcgccgccg ctgcccgagc gggtcttgcg cgcgatgccg
      661 ggctggcaac ggcatgcgca tcgcgccacc cacgcgctgc tctacggcct gttcttcgcc
      721 gtgccgctct cgggctgggc ctacagctcg gcggcgggct tccccgtcgt ctggttcggc
      781 gtgctgccgc tgcccgactg ggtggcggtc gacaaggact tcgccgacgc ggtgctcaag
      841 cccttgcacc agacctgcgc gttcgcgctg gccggcctcg tcgtgctgca cgtggcggcg
      901 acgctcaagc accagttcgt cgaccgcgac gggctgttct ggcgcatgtg gccgcgagga
      961 acggggcggt gaaggcgctc atcgcgctcg gcctcgtcgc cggcgccggg catgcccagc
     1021 cggtcgcgac gctcgtgccc gcgcaaagcg agatcgtgtt cacgagcaag cagatgggcg
     1081 tgccggtcga gggtcgcttt cggcgcttcg acgtgaaggt cgcgctcgac ccgaagcagc
     1141 ccgccggtgg cagcgtcgcg ctcacgatcg acactgcgag cgcgaccctg ggactgcccg
     1201 agaccgacgc cgagcttgcc cggccgaact ggttcgacac cgcgaagttc gcgcaggcca
     1261 cgttcaactc gagcagcatc aaaggcctgg gcggcggccg gttcgaggtc gccggcacgc
     1321 tgtcggtcaa gggcagcgcg cagccggtcg tcgtcccggt cacgttgacg cagtccgcag
     1381 gcaccgcggt cgcgaccggc gccttcacgg tcaagcggct ggcctaccgg atcggcgagg
     1441 ccgagtgggc cgacacctcg ctggtcgcgg acgacgtgca ggtgcgcttc aggctcgcgt
     1501 tcagcgggct ggcgccgccg tgacgcgcgc cccgcggcac ctcacttctt tttcttggcg
     1561 tcctggtcga cggccttcga ggccgaggtc ttgccaccct gcacgcccag ggtgccgccg
     1621 gtgccgaggc cgcccttgac ggtctgcgcc ttgtagttgc gcaccgccct gaccagctgg
     1681 ttgtagctgt cggcgaacgc ggcgatgatg atcttgcctt cgggcgtgtt gctgtagccg
     1741 cccgcgccgg cgccgccggc ccagccgaag gtgctgccga agacgttgaa gtcggtgttc
     1801 ttggcgctgc cctcggccgc cgcgagctgc acgcccgagc ggttgtcgat catgatcagc
     1861 gtggtcgagg cctcgttggt cttgaggccg ccggccagcg cgcccaccac accgaggctg
     1921 cggctgaagc cggccagccc gcccccgacc ccgccggtgt tctggctgaa ggtgatctgc
     1981 gggttcatcg tgtagtcggc cgcgaccatc tggcctgcgc cgaagttgct gccggcgcgc
     2041 atctcgcccg acttctgcag gttgcgctcg gtgttcatgt tgtgcatcgc cgcgccgcgc
     2101 tcgaccacga cgaagcagtt gctctgctgg atcatcatgc gcagcaccgg cacggtggag
     2161 ccgagcttgt agctgcgcag cgtctggtac cagccggcgt tctggtcttc gaccacgccg
     2221 atcgttccca gcgatgcgtc gcagcgctcc aggttgccgc tggcaccctc ggcggtcgcg
     2281 ccgccggcgg cgccggtcac cgcggtcttc gaggcgccct gctcgggctg tcccggcatc
     2341 gtggcgacgc acgcgctcag catgaacccg aggctgccgg ctgcggccag ccgcagccct
     2401 gccgcgccgt ggcgggcgcc ggatgtccaa cgatctgcgt tcataacatc ttcctacgct
     2461 ggttaccagc cgctcactat gagccgcttg cgctggcgat ggtaaccgtc gcgttggcgt
     2521 gattccaccc ccaacacgcc agtatttggg cgccggcgga acacgcttca gcgcgtcgcg
     2581 aggtactccg agaagatgcc gagcgccacc tcgcggcgca cctcgcgcag gccggcgcct
     2641 tcgagcgtgc gcatcaccgc ctcaggtgcg gcgcaggcct cgatcgtgtc ccagtaatag
     2701 cgccacagca tcggcgtggc cggctggcgc gcgaccagcc gcgccagcag cggcaccgcg
     2761 ccgcgcatgt aggccttcag cagcgcgttg gcgagcgcgc cgcgcggctt cgagatctcg
     2821 agcaccagca gcgtgccgcc cgggcgcagc acgcggtgga actcgcggaa cgcggcttcg
     2881 aggtcggtga tgtggcgcag cgcgtagccc atgacgagga agtcgacgct cgcgtcgggc
     2941 accggcaggc gctcggccgt gccccgcagg cagcgcacgg tcgccggcag ccgcgcctgc
     3001 gccatcatgc cggggctggg gtcgacgccg atcaggcgct gcggctcgtt cgtcagcgcc
     3061 aggatctcgt gcgcgaccag gccggtgccc atgcccacgt cgagcacgtc ggcgccgggc
     3121 ttcagcccgg cgcgctgcag ggcggttcgg cggtaccacg agccgctgcc gaagccgagc
     3181 agcttctcga ggcggttgta gtccggcgcg gtgctgtcga aggtctcgcg caggaactgc
     3241 gcgcgcgccg gcgcgtcggc gtagtagtcg ggcagcgggg tgtgcggcag gtggaccaga
     3301 tccggcgtgc cggagtcagc gggatccgga gcggcgccag gggcggctga agtcggtggc
     3361 atgcgggcgc ttctcgtcgt tgttgtcgtc gtcgtcgacg ggcatcgtac cgcgccggcc
     3421 gcgggcgcct gctttggtaa actcgtgcgc cgtgtgacga tggccgttcc ggtctcacga
     3481 ttttctttcc gacgatgccg gcccggcccg cgtcgccagc agcccgcgaa ccccacggac
     3541 ccgaagcacg catgaccacc gcccaaaccg atctgcagcg cgaagtggcc gcgctgatgg
     3601 ttgaagccct caacctcgac gtcgcgcccg agagcatcgc ccccgacgcg cccctgtacg
     3661 gcgaaggcct ggccctcgat tcgatcgaca tcctcgaagt cgcgctcgtc gtttcgcagc
     3721 gctacggctt ccagctgcgc tcggacgacg aagacaacgt gcgcatcttc acctccctgg
     3781 cgagcctgac cgagcacatt gccgcgcacc gcgtgagctg agcccagcat ggcaccccgc
     3841 ctcgggatcc gcctcgtcgc gatcgcgctg gccagcgtcg cctacatcct gggctgccac
     3901 tggctgatga cgcaggcgca cgcgtcgcca tggaacgcgg tcggcgtgct caccccaatg
     3961 ctcgcggcga tcgcgatcgg cgcctggcgc gctggccagc acctgccggc cgggctcgcg
     4021 gcgctcgcga tcggcgggct ctgcgcgcag gcgctgttcg gtgtacaggt gccgccgccg
     4081 ctgctctacc tggcgcagca cgccggcatc cacctgttcc ttgcaggcgt gttcggcagc
     4141 acgctgcgtg ccggacacac gccgctgatc acgacgctgg cgcggcgcgt gcaccgccgc
     4201 ttcacgcccg agatggccgc ctacacccgc aagtgcacga tcgcctgggt cgggtacttc
     4261 gtcgcgatgg cggtggcgtc ggtcgtgctc tacgcgttcg ctgcgttcga cacctgggcg
     4321 ctgttcgcca acctggtgac accggtcacg ctggcggcga tgttcggcgc agagtacctg
     4381 ctgcgctacc ggctgcaccc cgagttcgaa cgcgcgagct ttgccgacgc gatccgctcg
     4441 tacctgcaca aagccgccga ctgacccgag ccgcgatggc gctgccgctg atcctcgccg
     4501 acgacctcga cgccccgctt gcctggcgcg acggcatgcc cgtctcgcgc cgccagtacc
     4561 tcgccgacgt ggccgcgctc gccgcgcgcc tgcccggggc cgggccgatg ctgaacttca
     4621 gcgccgaccg ctaccgtttc gcggtcggcc tcggcgcagc aatgcggtgc ggccaggcca
     4681 gcctgatgcc gccgaaccac acgcccgaca cggtcgcgcg gctgcgcacg ctgttccccg
     4741 ccacctatgc actcgcggat gccggcgcgc cggcgctgca actgccgacc ctgcatcacg
     4801 ccgccgcgcc tgccaccgcg gccgaggtcg ccgcgcagac cgccgtgccc gagatcgacg
     4861 aggccgcg
//
LOCUS       DASRDG010000302         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_754060, whole genome shotgun sequence.
ACCESSION   DASRDG010000302 DASRDG010000000
VERSION     DASRDG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754060"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="ugpQ"
                     /locus_tag="VFA35_05020"
     CDS             <1..627
                     /gene="ugpQ"
                     /locus_tag="VFA35_05020"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZT55566.1"
                     /translation="KLSADGVPFLLHDAELERTSSGTGIASQLDWGELARLDAGGWHS
                     RGFAGEPLPSLDGIARYCIRNGFALNIEIKPTPGDEARTGRVVAAAAARLWAGHATPP
                     LLSSFRPDALQAALEAAPALPRALLLDTLRDGWFDEAGALGCVAVVTNFKVMDAALIA
                     KLHAARLRALVYTVNDPAEARRLAALGIDGLITDAVDRFSPGASGVHD"
     gene            complement(610..1212)
                     /locus_tag="VFA35_05025"
     CDS             complement(610..1212)
                     /locus_tag="VFA35_05025"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZT55567.1"
                     /translation="MEPLLEDVPERIETERLVLRMTRGDDATVLNAAVCESLDDLRAY
                     MPWAQAAPSLAQSHADCRRMQAKFLLREDLAMFMFERGADGSEGAFIGGTGLHRIDWI
                     VRRFELGYWCRTSRQGRGCVTEAAQALTRFAFDRLRARRVEVRMDDGNERSWKVAERA
                     GFALEGVLRSDSLTPLGAPRDTRVYALIGAGPASESIVNA"
     gene            complement(1227..>2477)
                     /locus_tag="VFA35_05030"
     CDS             complement(1227..>2477)
                     /locus_tag="VFA35_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT55568.1"
                     /translation="SILISSFGGQVTMLALPLTAAVLLHASPTQMGLLTAMEIAPFVL
                     FSLPVGVWLDRVRKLPVYVAGEALLALVVASVPLAWWMGWLSMGWLYVCGFLIGTTAT
                     IAGSAAQIVLTQVVARERLVEAHAKNALAVSGAEVAGPALAGALIKLVGAPLALLVDA
                     VLLLVSALILRGIVVFEVLRPRADAHFMRDLRAGVRFVAQHRLLVALACAVGIWQMCH
                     NAATVVQILFATRTLGLSEQAVGLSYMGMGLGTIVASVFGSRVSRRIGPGPCLVLGFA
                     VCGAGWLLLAVAPANAFGVAAFALMLMLFSTGAVLVFINFLALRQAVTPPHLLGRMTS
                     TMRWLILIPAGPGALLGGWLGEHFGLRSALAFAGSSALLLALVAGRAAVIRNVRSLPK
                     PEVERDWIGAEADVRPARPAVEPV"
BASE COUNT          402 a          957 c          820 g          298 t
ORIGIN      
        1 aagctgagcg ccgacggcgt gccatttctg ctgcacgacg ccgagctcga acgcacgtcg
       61 tccggcacgg gcatcgcgtc gcagctggac tggggcgaac tcgcgcgcct cgacgccggc
      121 ggctggcaca gccgcggctt cgcgggcgaa ccgctgccaa gcctcgacgg catcgcccgg
      181 tactgcatcc gcaacggctt cgcgctgaac atcgagatca agccgacgcc gggcgatgag
      241 gcgcgcaccg gccgcgtcgt cgccgccgca gccgcgcggc tctgggccgg gcacgccacg
      301 ccgccgctgc tcagttcgtt ccgccccgac gcgttgcagg ccgcccttga agcggccccg
      361 gccttgccgc gggccttgct gctcgacacc ttgcgcgacg gctggttcga cgaggccggg
      421 gcgctgggct gcgtcgcggt cgtcacgaac ttcaaggtca tggacgcggc gctgatcgcg
      481 aagctgcacg ccgcgaggct gcgcgcgttg gtctacaccg tcaacgaccc ggccgaggcg
      541 cgccggctcg ccgcgctcgg catcgacggc ctgatcaccg acgcggtcga ccgcttctcg
      601 cccggcgcgt caggcgttca cgattgactc gctggccggc cctgcgccga tcaacgcata
      661 gacccgcgtg tcgcgcggtg cgcccagcgg cgtcaggctg tcgctgcgca gcacaccttc
      721 gagcgcgaag ccggcgcgct cggccacctt ccagctgcgc tcgttgccat cgtccatgcg
      781 cacctcgacg cgccgcgcac gcagccgatc gaacgcaaac cgagtcagcg cctgcgccgc
      841 ctcggtgacg caaccgcgac cctgccggct ggtgcgacac cagtagccga gctcgaaacg
      901 ccgcacgatc cagtcgatgc gatgcaaccc ggtaccgccg atgaacgcgc cttcgctgcc
      961 gtcggcgccg cgctcgaaca tgaacatcgc caggtcttcg cgcagcagga acttcgcctg
     1021 catgcggcgg cagtcggcgt ggctctgcgc cagcgagggc gcggcctgcg cccacggcat
     1081 gtaggcacgc aggtcatcga gcgattcgca caccgcggca tttagcaccg tggcatcgtc
     1141 gccgcgcgtc atgcgcagga ccaggcgctc ggtctcgatg cgctcgggga cgtcttccag
     1201 caagggttcc atcgtcgtct cctccatcaa acaggttcga ccgccgggcg cgccggccgc
     1261 acgtcggcct cggcgccgat ccagtcgcgc tcgacctccg gcttcggcag gctgcgcaca
     1321 ttgcggatca ccgcggcgcg ccccgccacc agcgcgagca gcaacgcgct gctgccggcg
     1381 aacgccagcg ccgagcgcag cccgaagtgt tcgccgagcc agccaccgag cagcgccccc
     1441 ggcccggccg ggatcaggat cagccagcgc atcgtgctcg tcatccgccc gagcaggtgc
     1501 ggcggcgtga ccgcctggcg cagcgcgagg aagttgatga acaccagcac cgcgccggtc
     1561 gagaacaaca tcagcatcag cgcgaaagca gccacgccga acgcgttcgc cggcgccacc
     1621 gcgagcagca gccagcccgc accgcagacc gcgaagccga gcaccaggca cggccccggc
     1681 ccgatgcgcc ggctgacccg gctgccgaac acgctggcga cgatcgtgcc caggcccatg
     1741 cccatgtagc tcaggccgac ggcctgttcc gacagcccca gcgtgcgcgt tgcgaacagg
     1801 atctgcacga cggtcgccgc gttgtggcac atctgccaga tgccgaccgc acacgccagc
     1861 gccaccagca gccggtgctg cgcgacgaag cgcacgccgg cacgcaggtc gcgcatgaag
     1921 tgcgcgtcgg cgcgcggccg cagcacttcg aagaccacga tgccgcgcag gatcagcgcc
     1981 gacacgagca gcagcaccgc gtcgaccagc agcgcgagcg gcgcgccgac gagcttgatc
     2041 aacgcgccgg ccagtgccgg ccccgcgacc tcggcgcccg agaccgcgag cgcgttcttc
     2101 gcatgcgcct cgaccagccg ctcgcgcgcg acgacctggg tcagcacgat ctgcgcggcc
     2161 gagcccgcga tcgtcgcggt cgtgccgatc aggaagccgc acacgtacag ccagcccatc
     2221 gacagccagc ccatccacca cgcgagcggc acgctcgcga caacgagcgc gagcagcgcc
     2281 tcgccggcga cgtagaccgg cagcttgcgc acccggtcga gccagacgcc gaccggcagc
     2341 gagaacagca cgaagggcgc gatctccatc gccgtcagca gacccatctg cgtcgggctc
     2401 gcgtgcagca acacggccgc cgtcagcggg agtgcgagca tggtcacctg cccgccgaac
     2461 gagctgatca ggatcga
//
LOCUS       DASRDG010000303         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_406328, whole genome shotgun sequence.
ACCESSION   DASRDG010000303 DASRDG010000000
VERSION     DASRDG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406328"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="era"
                     /locus_tag="VFA35_05035"
     CDS             complement(<1..732)
                     /gene="era"
                     /locus_tag="VFA35_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZT55569.1"
                     /translation="MTNDTEDTSPSNAPAPATQRCGLVAIVGRPNVGKSTLLNALVGQ
                     KVSITSAKAQTTRHRIIGIRTVGESQFVFVDTPGFQTKHSAALNRTLNRTVTGVLGDV
                     DVVLFVVEAGRFGLDDAKVLALMQGIGKPVFLIANKLDTVLRRAELAPWLKGMQERHA
                     FAEFVPLSARREADVQRLLGIVKPYLPEQAWLHDEDALTDRNERFLASEIIREKLFRL
                     TGDELPYTSTVVIDEFKEEGELRRIS"
     gene            complement(742..1446)
                     /gene="rnc"
                     /locus_tag="VFA35_05040"
     CDS             complement(742..1446)
                     /gene="rnc"
                     /locus_tag="VFA35_05040"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZT55570.1"
                     /translation="MDTRLDALQRRIGHRFDDARLLSRALTHRSFGADHNERLEFLGD
                     AVLSLVISGLLFERFAGSDEGDLTRVRAHLVREESLHRVALQLALPEVLHLGEGEARG
                     GGAQRASILADALEALIGATFLDGGFDAARTAVTGLFGEIIATTDIASWSKDAKTELQ
                     EWLQARRLPVPTYRIRATHGQAHAQTFEVECVVPALSLVEQGVGRSRRVAEQEAARRM
                     LAALKRSDQPGGPLQS"
     gene            complement(1460..1828)
                     /locus_tag="VFA35_05045"
     CDS             complement(1460..1828)
                     /locus_tag="VFA35_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HZT55571.1"
                     /translation="MRVERQSARRQRGVTLFGLLLWAIVVGAVALIVMKVLPTMNEYF
                     TIQKAVNKIASEGATTVPEIRAAFERAKDIEYSIQSITAKDLTITKENDKVVISFAYD
                     KEIELIKPVFLLIKYEGRSR"
     gene            complement(1874..>2198)
                     /gene="lepB"
                     /locus_tag="VFA35_05050"
     CDS             complement(1874..>2198)
                     /gene="lepB"
                     /locus_tag="VFA35_05050"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZT55572.1"
                     /translation="DEDQRQYFPEYLERLGDVTHRILLNTHSQPFYGPEDKITFPFRE
                     NCRYSAEGVTCKVPPGNYFMMGDNRDNSQDSRFWGFVPDENIVGKAFWIWMNFSSPSR
                     FGGFH"
BASE COUNT          341 a          725 c          745 g          387 t
ORIGIN      
        1 agagatgcgg cgcaactccc cctcctcctt gaactcgtcg atcacgacgg tcgaggtgta
       61 gggcagctcg tcgccggtga gccggaacag cttctcgcgg atgatctcgc tggcgagaaa
      121 gcgctcgttg cgatcggtca gcgcatcttc gtcgtgcaac cacgcctgct caggcaggta
      181 gggcttgacg atgccgagca gccgctgcac atcggcctcg cggcgcgccg acagcggcac
      241 gaactcggcg aacgcgtggc gctcctgcat gcccttgagc caaggcgcca gttcggcgcg
      301 gcgcagcacg gtgtcgagct tgttggcgat caggaagacg ggcttgccga tgccctgcat
      361 caacgccagc accttcgcat cgtccaagcc gaagcggccg gcttcgacga cgaaaagcac
      421 cacgtcgacg tcgcccagca cgccggtcac ggtgcggttc agcgtgcggt tcagcgccgc
      481 actgtgcttg gtctggaagc ccggcgtgtc gacgaacacg aattgcgact cgccgaccgt
      541 gcggatgccg atgatgcggt gccgcgtggt ctgcgccttg gccgaggtga tgctgacctt
      601 ctgcccgacg agcgcgttca gcaaggtcga cttgcccacg ttcggccggc cgacgatcgc
      661 gacgaggccg cagcgttgcg tggccggcgc gggggcgttg gagggcgagg tgtcttcggt
      721 gtcgttggtc atggtcacgg ctcaagattg gagcgggccg ccaggctggt cgctgcgctt
      781 gagcgcggcg agcatgcggc gggccgcttc ctgctcggcg acgcggcgcg agcggcccac
      841 gccttgctcg accaggctca aggccggcac cacgcactcg acctcgaagg tctgcgcgtg
      901 cgcctggccg tgggtcgcgc ggatgcggta ggtcggcacc ggcaggcggc gcgcctgcag
      961 ccactcctgc agttcggtct tcgcgtcctt cgaccagctc gcgatgtcgg tcgtcgcgat
     1021 gatctcgccg aacaggccgg tgaccgcggt gcgcgcggcg tcgaagccgc cgtcgagaaa
     1081 ggtcgcgccg atcagcgctt cgagcgcgtc ggccaggatc gacgcgcgct gcgcgccgcc
     1141 gccgcgggcc tcgccttcgc ccagatgcag cacctcgggc agcgcgagct gcagcgcgac
     1201 gcggtgcagg ctctcctcgc gcacgaggtg ggcgcgcacg cgggtcaggt cgccttcgtc
     1261 ggaaccggcg aaacgctcga acagcaggcc cgagatcacc aggctgagca ccgcgtcgcc
     1321 caggaactcg agccgctcgt tgtggtcggc gccgaagctg cggtgcgtca gcgcgcggct
     1381 cagcaggcgc gcatcgtcga agcggtggcc gatgcgccgc tgcaatgcgt ccaggcgggt
     1441 gtccatcgtc ggctcgagcc tacctggagc ggccctcgta cttgatcagc aggaagaccg
     1501 gcttgatcag ctcgatctcc ttgtcgtagg cgaagctgat gacgaccttg tcgttctctt
     1561 tggtgatggt caggtccttc gcggtgatcg actggatcga atactcgatg tccttggcgc
     1621 gctcgaacgc ggcgcggatc tcgggcaccg tggtcgcacc ctcgctggcg atcttgttca
     1681 cggccttctg gatcgtgaag tactcgttca tggtcggcag caccttcatc acgatcagcg
     1741 cgaccgcgcc gacgacgatg gcccacaaca acaggccgaa cagcgtgacg ccgcgttgcc
     1801 gcctggccga ttgacgctca accctcatgc tcgaatgctc ctgtgtcgct gcgacccgcc
     1861 attctcgacc gggtcaatgg aacccgccga accgactcgg gctgctgaag ttcatccaga
     1921 tccagaatgc cttgccgacg atgttttcgt cgggcacgaa accccaaaaa cgtgaatcct
     1981 gggagttgtc gcggttgtcg cccatcatga agtagttgcc cggcggcacc ttgcaggtca
     2041 cgccctcggc actgtagcgg cagttctcgc ggaacggaaa ggtgatcttg tcctccggcc
     2101 cgtaaaacgg ctggctgtgc gtgttcagca ggatgcggtg cgtcacgtcg ccgagccgct
     2161 ccaggtactc gggaaagtac tggcgctggt cttcgtcg
//
LOCUS       DASRDG010000304         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_617554, whole genome shotgun sequence.
ACCESSION   DASRDG010000304 DASRDG010000000
VERSION     DASRDG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617554"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFA35_05055"
     CDS             <1..353
                     /locus_tag="VFA35_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55573.1"
                     /translation="LSAFGIPTPNCFCDLQPGTILAAIVVPFVPETDPLVQPRGGIAD
                     FRLHLPNVSWVLGTQLATQWFELSQMASSNTWLWSVANAAPTLDMALVEGHPSEAKGE
                     VSVHLAHVLRLEYQ"
     gene            356..634
                     /locus_tag="VFA35_05060"
     CDS             356..634
                     /locus_tag="VFA35_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55574.1"
                     /translation="MKVLAIILSAGVVAGLAVFTLLDVLAQLTVAGPLLTLPINAFIA
                     TLCGACFGAAGLALVFRAAMARADRSAMRKLEDRIARLEGRGVIEKAG"
     gene            complement(642..2114)
                     /locus_tag="VFA35_05065"
     CDS             complement(642..2114)
                     /locus_tag="VFA35_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZT55575.1"
                     /translation="MLEGLGFAAADRQKDVAVLSGGERSRAQLAVLMTTPADLLILDE
                     PTNHLDLAGIEFVEDFVRRYPGAVIAISHDRRFLDAVAQSIVVVGDGVAARYKGNFSH
                     YAAQRDLELLTQARQYRNQREFVDKEMDYIRRNMAGRMSAQAKGRLKRLQRLQLIEKP
                     KGTRATMRLSLGDAKGLRGQTVLDAEELTVRVDQRTLIDRGELRVWFGETVALLGRNG
                     AGKTTLLRLLAGLGAPAGGRIRRAPNLRIGYFSQEVTDLPQQGTVLDALRELDRTVDE
                     KQLRDHLALFLFCGDDVDKPVAELSGGEKQRLSLARLLRSQFDLLCMDEPTNHLDVAG
                     TEGLEQALKEYPGTVVLITHDRRLVEEVADRVLWVENGAIRTFERGLEQCLRVLAEER
                     SAARAAETEAREKAARKAQQAAAPAPKAIETGKVRNPLMFQKLEERIMALEEQLKQAR
                     DAMMLPENYASTSRMKELQQKEAALQQQLAEAYQQWENWQ"
BASE COUNT          277 a          787 c          725 g          361 t
ORIGIN      
        1 cgctgtcggc gttcggcatc ccgacgccga actgcttctg cgatctgcag cccggcacca
       61 tcctggcggc gatcgtcgtg cctttcgtgc ccgaaacgga tccgctggtg cagccgcgcg
      121 gcggcatcgc cgacttccgg ctgcacctgc cgaacgtcag ctgggtcctc ggcacgcagc
      181 tcgcgaccca atggttcgag ctgtcgcaga tggcgagcag caacacctgg ctctggagcg
      241 tcgccaatgc ggcgcccacg ctcgacatgg cgctcgtcga gggccacccg agcgaagcga
      301 agggtgaggt gagcgtgcac ctcgcgcacg tgctgcggct cgagtaccag taaccatgaa
      361 ggtcctcgcg atcatcctct cggccggcgt ggtcgccggt ctcgccgtct tcaccctcct
      421 ggatgtgctg gcgcagctca ccgtggcagg gccgctgctc acgctgccga tcaacgcgtt
      481 catcgcgacg ttgtgcgggg cgtgcttcgg cgcggcgggg ctcgcgctcg tgttccgcgc
      541 cgccatggcg cgcgccgacc gcagcgcgat gcgcaagctc gaagaccgca tcgcgcggct
      601 cgagggtcgg ggcgtgatcg agaaggccgg ctgacagcgc gctactgcca gttctcccac
      661 tgctggtacg cctcggcgag ctgctgctgc agcgccgctt ccttctgctg cagctccttc
      721 atcctgctcg tcgacgcgta gttctcgggc agcatcatcg cgtcgcgcgc ctgcttcagc
      781 tgctcctcga gcgccatgat gcgctcctcg agcttctgga acatcagcgg attgcgcacc
      841 ttgccggtct cgatcgcctt cggcgccggc gcggcggcct gctgcgcctt cctcgccgcc
      901 ttctcgcgcg cctcggtctc ggcggcgcgc gcggccgagc gctcctcggc gagcacgcgc
      961 agacactgct cgagcccgcg ctcgaaggtg cggatcgcgc cgttctcgac ccacagcacc
     1021 cggtcggcga cctcctcgac gagccgccgg tcgtgcgtga tcagcacgac cgtgcccggg
     1081 tactccttga gcgcctgctc gagaccttcg gtgccggcga cgtcgaggtg gttggtcggc
     1141 tcgtccatgc acagcaggtc gaactgcgaa cgcagcaggc gcgcgagcga caggcgctgc
     1201 ttctcgccgc cgctcagctc ggcgaccggc ttgtcgacgt cgtcgccgca gaacaggaac
     1261 agcgcgaggt ggtcgcgcag ctgtttctcg tcgacggtgc gatcgagctc gcgcagcgcg
     1321 tcgagcaccg tgccctgttg cggcaggtcg gtgacctcct ggctgaagta gccgatgcgc
     1381 aggttcggcg cgcgccggat gcggccgccg gccggcgcgc cgaggccggc gagcagccgc
     1441 agcaacgtcg tcttgccggc gccgttgcgg cccagcagcg ccaccgtctc gccgaaccag
     1501 acgcgcagct cgccgcggtc gatcagcgtg cgctggtcca cgcgcacggt cagctcctcg
     1561 gcgtcgagca ccgtctggcc gcgcaggccc ttcgcgtcgc cgagcgacag ccgcatcgtc
     1621 gcgcgcgtgc ccttcggctt ctcgatcagc tgcagccgct gcagccgctt gagccggccc
     1681 ttcgcctgcg ccgacatgcg ccccgccatg ttgcggcgga tgtaatccat ctccttgtcg
     1741 acgaactcgc gctggttcct gtactggcgc gcctgcgtca gcagctcgag gtcgcgctgc
     1801 gccgcatagt gcgagaagtt gcctttgtag cgcgcggcca cgccgtcgcc gacgacgacg
     1861 atcgactgtg cgaccgcgtc gaggaaccgg cggtcgtggc tgatcgcaat gaccgcgccc
     1921 ggataacggc gcacgaagtc ctccacgaac tcgatgccgg cgaggtcgag gtggttggtc
     1981 ggctcgtcga ggatcagcag gtcggcgggc gtcgtcatca ggaccgcgag ctgcgcgcgc
     2041 gaacgctcgc cgccgctcag caccgcgacg tccttctgcc ggtccgccgc cgcgaagccg
     2101 agcccttcga gcacgcccta gaccttgtgg tcgtggtcgt agccgccgcc
//
LOCUS       DASRDG010000305         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_277597, whole genome shotgun sequence.
ACCESSION   DASRDG010000305 DASRDG010000000
VERSION     DASRDG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277597"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..676)
                     /locus_tag="VFA35_05070"
     CDS             complement(107..676)
                     /locus_tag="VFA35_05070"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HZT55576.1"
                     /translation="MQAIAGIQDVLGSWRRLGRHRLPNGTELIAQAPDGDGSQWLHAI
                     YPKLSSEEIRELESAVGRPLPLDLRTFYRTIGGLSLFCGAFCIHSRGSHYLTLGEEAL
                     RPPDLARFNHELDSAGWLPADAVAFAENGWDLTVHVCGMGATPTEVVRVDRHTGELLQ
                     RHPTIWNLVAEKLLTYEHLLLGDAARPRV"
     gene            885..1637
                     /locus_tag="VFA35_05075"
     CDS             885..1637
                     /locus_tag="VFA35_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZT55577.1"
                     /translation="MEIVVCIKRVPTTDTAIKIAADGRSIDTQGVQFELNAYDEFALE
                     QALRIKEQLNAGSVTVVTLGPKEAQKELRDALARGADRAVLLVTDKWVHDSWSCAQAL
                     VAWLRKVKCDLVLCGKQAVDADNNQLPPRLAAMLGFACVTEAGKLLLANGAFTAERDI
                     EGAREVVACRTPAVISCNKGLNEPRYANLKGIMAAKKKPFEELPTALAEPAMAIESLT
                     LPASRKGGRILGNGVDAIPALIGALRNEAKVL"
     gene            1690..>2596
                     /locus_tag="VFA35_05080"
     CDS             1690..>2596
                     /locus_tag="VFA35_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZT55578.1"
                     /translation="MSNIVVVAEVRAGKLKRPSLEAITAAQQLAAKCGGKVAAVAVGA
                     GLDAAGAELARCAAQVVLADAPAFAAYSGDAYARVVAEQCKALAASAVLMPHTAMGKD
                     LLPRVAALLDTGLVTDAIGVDWQGGKFAAQKPVFAGKALLKVTAARTPFMATLRPNNF
                     AAATGGGSGAAPQKTAPAAGELLSVVKEIVASGADKVPLQEAKVIVAGGRGLKGPEHY
                     HLIEKLAAAFGPGVAAVGSTRAVVDAGWRPHSEQVGQTGKVVSPTLYFAIGISGAIQH
                     LAGMRTARTIVAINKDPEAPIFKVAD"
BASE COUNT          400 a          925 c          930 g          341 t
ORIGIN      
        1 aggcgggttc gcctcgggat tccttctcag tctccgagcc gaacacgccg ggcgggtgcg
       61 cctcgggatc ctccctcact ccctgacgcg cggcgaccgt cggcggctag acgcgcggcc
      121 gcgccgcgtc gccgagcagc aggtgttcgt aggtgagcag cttttcggcg acgaggttcc
      181 agatcgtcgg gtgccgctgc agcagctcgc ccgtgtggcg gtccacgcgc acgacctcgg
      241 tgggcgtggc gcccattccg cagacgtgca cggtgagatc ccagccgttc tccgcgaacg
      301 ccacggcgtc ggccggcagc cagcccgcgg agtcgagttc gtggttgaag cgcgcgaggt
      361 cgggcggccg cagcgcctcc tcgccgagcg tgaggtagtg cgagccgcgg ctgtggatgc
      421 agaacgcgcc gcagaacaac gacaggccgc cgatcgtgcg atagaaggtg cgcaggtcga
      481 ggggcagcgg ccgaccgacc gccgactcga gctcgcggat ctcctcgctc gacagtttcg
      541 gatagatcgc gtgcagccac tgcgagccgt cgccgtcggg agcctgcgcg atgagctcgg
      601 tgccgttggg cagacggtgc cgtcccagcc ggcgccagga cccgagcacg tcctggatgc
      661 cggcgatcgc ttgcatgacc ccacgcgtca tgggtcgagg atcggcaacc accgcggcgc
      721 cgacgcatca gggttttccg ccgttcgcgg ccccggcgcg gcaggcatgc gggcggccgg
      781 acacgaagcg gcggtcgcgc cgggaaaacg gtgcggaaaa ccggcggcgc aggctctaca
      841 atcgccgccc tttttcctgg accaacgact cgcaccggaa acccatggag atcgtcgtct
      901 gcatcaagcg cgtgccgacc accgacaccg cgatcaagat cgccgccgat ggcaggtcca
      961 tcgacacgca gggcgtgcag ttcgagctga acgcctacga cgagttcgcg ctcgagcagg
     1021 cgctgcgcat caaggagcag ctcaacgccg gttcggtgac cgtggtcacg ctcggcccca
     1081 aggaggcgca gaaagagctg cgcgacgcgc tcgcgcgcgg cgccgacagg gcggtgctgc
     1141 tcgtcaccga caagtgggtg cacgacagct ggtcgtgcgc gcaggcgctc gtcgcctggc
     1201 tcaggaaggt caagtgcgac ctcgtgctct gcggcaagca ggcggtcgac gccgacaaca
     1261 accagctgcc gccgcgcctc gccgcgatgc tcggcttcgc gtgcgtgacg gaggccggca
     1321 agcttctgct ggcgaacggc gccttcacgg ccgagcgcga catcgagggc gcgcgcgagg
     1381 tcgtcgcgtg ccggacgcct gcggtcatca gctgcaacaa gggcctcaac gagccgcgct
     1441 acgccaacct caagggcatc atggcggcga agaagaagcc gttcgaggag ctgccgaccg
     1501 cgctcgccga gccggcgatg gcgatcgaat cgctgacgct gccggcgtcg cgcaagggcg
     1561 gccggatcct cggcaacggc gtcgatgcga tccccgcgct gatcggcgcg ctccgcaacg
     1621 aagccaaggt cctctgaggc gcggacgcgc gccccgggtt cccttctcag ttcccacccg
     1681 aacgcatcca tgagcaacat cgtcgtcgtc gccgaagtcc gcgcgggcaa gctgaagcgc
     1741 ccgtcgctcg aagccatcac ggccgcccag cagctggcgg cgaagtgcgg gggcaaggtc
     1801 gcggcggtcg ccgtcggcgc cggtctcgac gccgccggcg ccgagctggc ccgctgcgcg
     1861 gcgcaggtcg tgctcgccga tgcgcccgcg ttcgccgcct actcgggcga cgcgtacgcg
     1921 cgcgtcgtcg ccgagcagtg caaggcgctc gccgccagcg ccgtgctgat gccgcacacg
     1981 gcgatgggca aggacctgct gccgcgcgtc gcggcgctgc tcgacacggg cctcgtcacc
     2041 gacgcgatcg gcgtcgactg gcagggcggc aagttcgcgg cgcagaagcc ggtgttcgcc
     2101 ggcaaggccc tgctcaaggt caccgccgcg cgcacgccgt tcatggcgac gctgcgcccg
     2161 aacaacttcg ccgccgcgac cggcggcggc agcggcgccg cgccgcagaa gaccgcgccg
     2221 gcggccggtg agctgctgtc ggtggtcaag gagatcgtcg cctcgggcgc cgacaaggtg
     2281 ccgctgcagg aggcgaaggt gatcgtcgcc ggcggccgcg gcctcaaggg ccccgagcac
     2341 tatcacctga tcgagaagct cgcggcggcg ttcggccccg gcgtcgccgc cgtcggcagc
     2401 acgcgcgccg tggtcgacgc cggctggcgc ccccacagcg aacaggtcgg ccagaccggc
     2461 aaggtcgtga gtccgacgct ctacttcgcg atcggcatca gcggcgcgat ccagcacctg
     2521 gcgggcatgc gcaccgcgcg cacgatcgtc gcgatcaaca aggatcccga ggcgccgatc
     2581 ttcaaggtcg ccgact
//
LOCUS       DASRDG010000306         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1429374, whole genome shotgun sequence.
ACCESSION   DASRDG010000306 DASRDG010000000
VERSION     DASRDG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429374"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2462)
                     /locus_tag="VFA35_05085"
     CDS             complement(<1..>2462)
                     /locus_tag="VFA35_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZT55579.1"
                     /translation="IVDEIHAVAGSKRGSHLALSLERLSSLVGRRLQRVGLSATQKPI
                     EEMARFLTGVTDPADAGVIIDSGHDRARDLALVLPDAPLEAVMSNEVWQTLYDKIAAL
                     IAEHRTTLVFANTRRMVERVTRHLSERIGAENVAAHHGSLAREQRLDAERRLKAGSLR
                     ALVATASLELGIDIGDVELVCQLATPRSIATFLQRVGRANHAVTGVPKGRLFPLSRDE
                     LVECTALLDAVRAGELDRLHIPPRPLDVLAQQVVAEVSAREYEMDELFAQVRRAWPYR
                     NLERTEFEEVVRMLAEGYAGRRGRQATYLHHDAVNHRLRARRGARLTAITCGGAIPSN
                     ADYQVVLEPQGIIVGTLNEDFAVESLAGDIFQLGNTSYQILRVEAGRVRVQDAAGQPP
                     TIPFWLGEAPARSNELSAAVSALRARIDELLPAVDAETVEKAILGTMHEHVLPEFAAR
                     QLVEYLAAAKAALGCVPTQQQLVIERFFDEAGGMQLVVHAPFGSRINRALGLGLRKRF
                     CQSFNFELQAAATEDAIVLSLGETHSFPLDEVPRYLNSRTVTHVLTQAVLDAPLFTTR
                     WRWNATVSLAVRTSRSGKKVPAPLLRMASEDLLSMVFPDQLACAENLRGEREVPEHPL
                     VRQTLRDCLCEAMDLDGLIALLQSIESGQRRVITQDLPHPSPLAMEILGARPYAYLDD
                     APLEERRTQAVAARRWLDPQSAAEFGKLDPQAVAQVRSEAWPTVESADELHDALMLLG
                     VLAEAEAPEWQPWFGHLLQEHRAARLVMPDATYCVAVEQLPMLGALYVRASVAPPSVV
                     PAEYAAREWTTDTACVELVRGRL"
BASE COUNT          392 a          835 c          849 g          386 t
ORIGIN      
        1 agccggccgc gaacgagctc gacgcaggcg gtgtcggtgg tccactctcg cgcggcatat
       61 tccgcgggaa ccactgaggg cggtgccacg ctcgcgcgaa cgtaaagcgc gccgagcatc
      121 ggcaactgct cgacggcgac gcagtacgtt gcatccggca tcaccagccg cgccgcccgg
      181 tgctcctgga gcaagtgccc gaaccagggt tgccactcgg gcgcttcggc ctccgcgagc
      241 acaccgagca gcatcagcgc gtcgtgcagc tcatcggcac tttcaacggt cggccacgcc
      301 tcagaacgca cctgtgcgac ggcctgcggg tcgagtttcc cgaactcggc ggcactctgc
      361 ggatcgagcc agcggcgggc cgcgacggcc tgcgtgcggc gctcttcgag cggagcatcg
      421 tcgaggtagg cgtaggggcg cgccccgagt atctccatgg ccagcggcga gggatgcggc
      481 aggtcctgcg tgatcacgcg gcgctgcccg gactcgatcg attgcagcag ggcgatgagt
      541 ccgtcgaggt ccatcgcctc gcacaggcaa tcgcgcagcg tctgccgcac cagcggatgc
      601 tcgggaacct cgcgctcacc gcgcaggttt tcggcacaag cgagctgatc ggggaagacc
      661 atcgacagca ggtcttccga tgccatgcgc agcagcggcg ccggcacctt cttgccgctg
      721 cgcgaggtcc ggaccgcgag cgacacggtc gcgttccagc gccagcgcgt cgtgaacaat
      781 ggcgcgtcga gcaccgcctg ggtcagcacg tgggtcacgg tgcggctgtt gaggtagcgc
      841 ggcacttcat cgagcggaaa gctgtgcgtt tcgccgagcg acaggacgat cgcgtcctcg
      901 gtcgcggcgg cctgcagctc gaaattgaag ctctggcaaa agcgcttgcg cagtccgagg
      961 ccgagtgccc gattgatgcg cgagccaaac ggcgcatgca ccaccaattg catgcccccg
     1021 gcctcgtcga agaagcgctc gatcacgagc tgctgctgcg taggcacgca tcccagcgcc
     1081 gctttggccg ccgccaggta ttcgaccagt tgccgggcgg cgaactcggg cagcacgtgc
     1141 tcgtgcatcg tgccaaggat cgctttctca accgtctcag cgtcgaccgc tggcagcagc
     1201 tcatcgatcc tggcgcgcag tgccgatacc gccgccgaca gttcgttgct gcgtgccggc
     1261 gcctcgccga gccagaacgg aatggtcggc ggctggccgg ccgcgtcctg gacgcgcacg
     1321 cggccggctt cgacacgcag gatctggtaa gaggtgttgc ccaactggaa gatatcgccg
     1381 gccaggctct cgacggcgaa atcctcgttc agcgtgccga cgatgatgcc ctgcggttcg
     1441 agcacgacct ggtagtcggc gttggaaggg atcgcacccc cgcaggtgat ggcggtaagt
     1501 cgggcgccgc gccgcgcgcg caaccggtga ttgacggcgt cgtggtgcag gtacgtggcc
     1561 tggcggccgc gccggccggc gtagccttct gccagcatgc gcaccacttc ttcaaattcg
     1621 gtccgctcga ggttgcgata cggccaggcg cggcgcacct gcgcgaacag ctcatccatt
     1681 tcatactcgc gcgcactgac ctcggccacg acctgctgtg ccagcacgtc gagcggccgc
     1741 ggcgggatgt gcagccggtc gagctcgccg gcgcgcaccg catcgagcag ggccgtgcat
     1801 tccacgagct cgtcgcgact caacgggaag aggcgcccct tcggcacgcc cgtgacggca
     1861 tgattggcgc gcccgactcg ctgcaggaac gtggcgatgg accgcggcgt ggcgagctgg
     1921 cacacgagtt ctacatcacc gatgtcgatg ccgagctcga gcgatgccgt cgcgacgagc
     1981 gcgcgcagcg aaccggcctt gaggcgccgc tcggcgtcca ggcgctgctc gcgcgccagg
     2041 ctaccgtggt gggcggccac gttctccgca ccgatccgct cgcttaaatg tcgcgtaacg
     2101 cgctcgacca tgcgccgcgt gttggcgaat acgagcgtgg tgcggtgttc ggcgatgagc
     2161 gcggcgatct tgtcgtagag cgtctgccag acctcgttgg acatcacggc ctcgagcggc
     2221 gcgtcgggta gcaccagtgc caggtcgcgc gcgcgatcat ggccgctgtc gatgatcacg
     2281 ccagcgtcgg caggatcggt caccccagtg agaaaacggg ccatctcctc gatgggcttc
     2341 tgcgtcgcgg acaggcccac gcgctgcagc cgccggccca cgagcgatga caggcgctcc
     2401 aggctcaggg ccaggtgcga gccgcgcttg ctgccggcga cggcgtggat ttcgtcgacg
     2461 at
//
LOCUS       DASRDG010000307         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_617876, whole genome shotgun sequence.
ACCESSION   DASRDG010000307 DASRDG010000000
VERSION     DASRDG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617876"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFA35_05090"
     CDS             complement(<1..559)
                     /locus_tag="VFA35_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HZT55580.1"
                     /translation="MQRIDAPRRRLLQIGAATALLGRAAFARAAETPAQLRIGFQKSA
                     VNLVILKQQGALEKRFPGARVSWIEFPAGPQLLEALAVGSLDFGLTGDSPPVFAQAAG
                     KDLLYVGAEPPKPLSSAILVKPDSPLRTLADLKGKRVAFQKGSSAHYLTVRAIEKAGL
                     QWNDFAPIYLAPADARAAFEKGAVDA"
     gene            complement(562..1584)
                     /locus_tag="VFA35_05095"
     CDS             complement(562..1584)
                     /locus_tag="VFA35_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT55581.1"
                     /translation="MAVSSPVSQAPGGAARRSWLRRAVVLGAAVSGLLLVGAVPAQTP
                     RAELRIGYQKAASLFVLQKAQGTLEKRLAALNVGVRWVEFPAGPQLLEGLNVGSVDVG
                     YVGEAPPIFAQAAGANFVYIGFEPAAPEAEAIVVVKDSPIRSVADLKGKKIALNKGSN
                     VHYLLVRALEKAGLKYADVQPVFLAPADARAAFEKGAVDAWAIWDPFLAAVQKQTGAR
                     IVADGRGGVANNFPFYLAERGFAAKHGDVIKALFSDAIEQGQWLKSNLKQAAAIIAPL
                     QGLDTEVVELALTRYQFGVAPLTPQVAAEQQKLADVFHELKLIPRPIRIADALPGSAA
                     VKTASN"
     gene            complement(1709..2323)
                     /gene="ssuE"
                     /locus_tag="VFA35_05100"
     CDS             complement(1709..2323)
                     /gene="ssuE"
                     /locus_tag="VFA35_05100"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0044273 - sulfur compound catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HZT55582.1"
                     /translation="MSLSIRTPPAMHALLIAASPSEHSRSAALLDAAGLALEAAGVGV
                     RRLALRELPAEALLRARTGTPELGAAVAQVASASAVVFATPIYKAAYSGLLKVFLDLL
                     PQDGLHGRWVWPLATGGSPAHTLALDHSLRPVLDNLGAHHVLPSVFALDAQVAPQAAG
                     GGALIDAAIQARLAAGAERLAGAFDAQRQLRASAAARAAARCSA"
     gene            complement(2320..3315)
                     /locus_tag="VFA35_05105"
     CDS             complement(2320..3315)
                     /locus_tag="VFA35_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT55583.1"
                     /translation="MTFGTAAAIAFGALPVRADTTLLNVSYDPTRELYQAFNAAFAKQ
                     WKARTGETVTIRQSHGGSGKQARAVIDGLEADVVTLALAYDIDAIHDHGNQIPADWQK
                     RLPNNASPYTSTIVFLVRKGNPKKITDWADLARSGVEVITPNPKTSGGARWNYLAAWG
                     YALKQPGGNAESAKNFVKRLYANTKVLDSGARGSTTTFVERGIGDVLIAWENEAYLSV
                     KELGADKFEIVTPSLSILAEPPVSVVDKVVDKRGTRRAATAYLDYLYSPEGQEIAAKN
                     YYRPRDAKVAAKYAGQFKPVKLLTIDADFGGWRAAQKTHFDDGGVFDQIYAPSAK"
BASE COUNT          485 a         1198 c         1257 g          516 t
ORIGIN      
        1 acgcatcgac cgcgcccttc tcgaacgcgg cgcgcgcgtc ggccggcgcg aggtagatcg
       61 gcgcgaagtc attccactgc aggccggcct tctcgatcgc gcgcacggtc aggtagtgcg
      121 cgctcgagcc tttctggaac gcgacccgct tgcctttcag gtcggcgagc gtgcgcaaag
      181 gcgaatcggg cttcacgagg atcgccgagc tcagcggctt cggcggttcg gcgccgacgt
      241 agagcaggtc cttgccggcc gcctgcgcga acaccggcgg cgagtcgccg gtcaggccga
      301 agtccaggct gccgaccgcg agggcttcga gcagctgcgg gcccgcaggg aactcgatcc
      361 agctgacccg cgcgccggga aagcgctttt ccagcgcgcc ctgctgcttc aggatcacga
      421 ggttcaccgc gctcttctgg aagccgatgc gcagttgcgc cggcgtttca gcggcgcgcg
      481 cgaacgcggc gcggccgagc agggcggtgg ccgcgccgat ctgcagcagg cggcggcggg
      541 gggcgtcgat gcgttgcatg ctcagttgct cgcggtcttg acggcggcgc tgcccggcag
      601 cgcatccgcg atgcggatcg gcctcgggat cagcttcagc tcgtggaaca cgtcggccag
      661 cttctgctgc tcggccgcga cctgcggcgt cagcggcgcg acgccgaact ggtagcgcgt
      721 cagcgcgagc tcgacgacct cggtgtccag cccctgcagc ggcgcgatga tcgcggcggc
      781 ctgtttgagg ttcgacttca gccactggcc ctgctcaatc gcatcgctga acagcgcctt
      841 gatcacgtcg ccgtgcttgg ccgcgaagcc gcgctcggcc aggtagaacg gaaagttgtt
      901 cgcgacgccg ccgcggccgt cggcgacgat gcgcgcgccg gtctgcttct gcaccgcggc
      961 gaggaacggg tcccagatcg cccaggcgtc gaccgcgccc ttctcgaacg cggcgcgcgc
     1021 gtcggccggc gcgaggaaga ccggctgcac atcggcgtac ttcaagcccg ccttctccag
     1081 cgcgcgcacc agcaggtagt gcacgttcga gcccttgttc agcgcgatct tcttgccctt
     1141 caggtcggcg accgaccgga tcggcgagtc cttcacgacc acgatcgcct cggcctcggg
     1201 cgcggccggc tcgaagccga tgtagacgaa attcgcgccg gccgcctgcg cgaagatcgg
     1261 cggcgcctcg cccacgtagc cgacatcgac cgagccgacg ttgagccctt cgagcagctg
     1321 cggcccggcc gggaactcga cccagcgcac gccgacgttc agcgccgcga gccgcttctc
     1381 gagcgtgccc tgcgccttct gcagcacgaa caggctggcg gccttctggt agccgatgcg
     1441 cagctcggcg cgcggcgtct gcgccggcac cgcgccgacg agcaacagcc ccgacacagc
     1501 ggcgccgagc accacggcgc ggcgcagcca gctgcggcgc gcggcgccgc cgggcgcctg
     1561 ggagacaggg gacgaaacag ccatggtcgg gttccttgaa agaaggtctc gcgcacgctg
     1621 cgcgggggcc gcgcacgcga gaccgaatcc gcctcgagag gcagaggaga cagacgggag
     1681 acgggcgcat cgcgccgcgt cgaggggatc aggcgctaca tcgcgcggcg gcgcgcgcag
     1741 cggccgatgc gcgcagctgg cgttgcgcgt cgaacgcgcc ggcgagccgt tcggcgccgg
     1801 ccgcgagccg cgcctggatc gccgcatcga tgagcgcgcc gccgccggcc gcctgcggcg
     1861 cgacctgcgc gtcgagcgcg aacaccgacg gcagcacgtg gtgcgcgccg aggttgtcga
     1921 gcaccggccg cagcgagtga tcgagcgcga gcgtgtgcgc cgggctgccg ccggtggcga
     1981 gcggccagac ccagcggccg tgcaggccgt cctgcggcag caggtcgagg aagaccttca
     2041 gcagcccgct gtacgccgcc ttgtagatcg gcgtggcgaa gacgaccgcg ctcgcgctcg
     2101 ccacctgcgc gaccgccgcg ccgagctcgg gcgtgccggt gcgcgcgcgc agcagcgcct
     2161 cggccggcag ctcgcgcagc gcgagccggc gcacgccgac gccggcggct tcgagcgcga
     2221 ggccggcggc atcgagcagc gcggccgagc gcgaatgctc ggacgggctg gcggcgatca
     2281 gcagggcgtg catggcgggc ggcgtgcgga tcgacaagct cacttcgcgc tcggtgcgta
     2341 gatctgatcg aacacgccgc cgtcgtcgaa gtgcgtcttc tgcgcggcgc gccaaccgcc
     2401 gaagtcggcg tcgatcgtca gcagcttgac cggcttgaac tggccggcgt acttggccgc
     2461 gaccttcgcg tcgcgcgggc ggtagtagtt cttcgccgcg atctcctggc cctcgggcga
     2521 gtacaggtag tcgaggtagg cggtcgcggc cctgcgcgtg ccgcgcttgt cgacgacctt
     2581 gtcgaccacg ctgacgggcg gctcggccag gatcgacagg ctcggcgtga cgatctcgaa
     2641 cttgtcggcg ccgagctcct tgaccgacag gtaggcctcg ttctcccacg cgatcagcac
     2701 gtcgccgatg ccacgttcga cgaaggtcgt ggtcgagccg cgcgcgcccg agtcgagcac
     2761 cttggtgttg gcgtacaggc gcttgacgaa gttcttcgcg ctctcggcgt tgccacccgg
     2821 ctgcttcagc gcgtagcccc aggcagcgag gtagttccag cgtgcgccgc ccgaggtctt
     2881 cgggttcggc gtgatcacct cgacgccgct cctggcgagg tcggcccagt cggtgatctt
     2941 cttcgggttg cccttgcgca ccaggaacac gatcgtcgag gtgtagggcg aggcgttgtt
     3001 gggcaggcgc ttttgccagt cggccggaat ctggttgccg tggtcgtgga tcgcgtcgat
     3061 gtcgtaggcg agcgcgagcg tcacgacatc ggcctcgagc ccgtcgatca ccgcgcgggc
     3121 ctgcttgccc gagccgccgt gcgactggcg gatcgtgacc gtctcgccgg tcctggcctt
     3181 ccattgcttc gcgaaggcgg cgttgaaagc ctggtagagc tcgcgggtcg ggtcgtagct
     3241 gacgttgagc agcgtggtgt cggcgcgtac cggcaacgcg ccgaaggcga ttgcagccgc
     3301 ggtaccgaac gtcagcgctc ggcgggagag gggtttcggg gtcataggca gtggcggatc
     3361 gatggacgaa ggccgcactg taggcagcgc ccccgctgcc gtgaaccaac aaatcctgct
     3421 tagcttttca cgcggtccag gatccacgcc gccgcg
//
LOCUS       DASRDG010000308         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_617932, whole genome shotgun sequence.
ACCESSION   DASRDG010000308 DASRDG010000000
VERSION     DASRDG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617932"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2121
                     /locus_tag="VFA35_05110"
     CDS             <1..2121
                     /locus_tag="VFA35_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55584.1"
                     /translation="LGFTRPTLFERRTDWSSTADLSRRIDLAPLDKTGSRMLANELLK
                     KLPEVPAALRELVTSSAEGNPFYMEELVKMLIDQGAIQTRSDGGEAWSVNAERLLVTQ
                     VPPTLTGVIQARLDGLPPAEKRALQQASIVGTVLWDQALAAIEAQAAELLPALVQREL
                     TLPRTDAALDGLRAGLREYAFRHQVLHQVTYDTVLKRHKREGHAKVAQWLAALTEHGS
                     LRAGDFLGTAAEHFEQAGDAPNAAEFHARAAEQAGQRFAHDRVLAHVGRALALLGETP
                     APPPAQAALRWRLLSARERTLNLQGRRDEQAADLNALEQLADLLDDDRRRAQAAWRRG
                     FRALRMADWAAMESAARHGLACAARAGDDELRLQALRLLASARVVQGDIEAGRALAQQ
                     GLAEARSLGLRGVEVRLLNALSIAAEMQGDLVGSLDLDRQNLLICRETGDRLNEAIAL
                     SNQGDGWLKLGDLAQSRRDLDAALQMLRANGDRTIEGAALANLSAVALWQGEETRALA
                     LARQALDIAVAAKARDFEVLAGLRLGAAELALGRRAAARDAYAQAHARAAEIDSARQH
                     DAIAGLARVALAEGDAAAAPAAMAALKPLLDHIAAGGTLDGTEDPRGIELSCHLALAR
                     TSDPRAADWLIRAHGALMAQADAISRSTTDARLRQGFLQNIPHHREIVERFGAWGGLG
                     SKAAPPSAVDRHQRPFVSVRVINL"
     gene            2338..2889
                     /locus_tag="VFA35_05115"
     CDS             2338..2889
                     /locus_tag="VFA35_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZT55585.1"
                     /translation="MVLAMAMLVLTTGARAGPTLEGDTVDAAMIRTIDTGYGLGRIVG
                     YGLEGPFVVVNGPADVQQYSEHYLLDIDGDHFTVDFLGQFTSGWQEGTVLRLSDLDFS
                     GASFLSGLDIDTNLVGYTLAFGSNYIDIGLGGTEFSGSTYFNATFKVSQVTAIPEPAT
                     LALMAVGLLGVASAAKRSRRRLR"
     gene            complement(3007..>3575)
                     /gene="gstA"
                     /locus_tag="VFA35_05120"
     CDS             complement(3007..>3575)
                     /gene="gstA"
                     /locus_tag="VFA35_05120"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HZT55586.1"
                     /translation="PHIALHEAGLPFEAVLASTKSHKLQDGTDYYTINPKGYVPLLEF
                     DDGTRLAEGPAIVQWIADQVPARRLAPPAGTMERYRLQEWLTFIGTEIHEQFSPLFNP
                     ALPEEAKPPLRQKIVDRLAYVDRHLAIREYVLGDFTVADGYLYTVARWAAPLKLDIAG
                     LEVLNAFMARMRARPAVQRALEAEGLPG"
BASE COUNT          588 a         1189 c         1269 g          529 t
ORIGIN      
        1 ctgggcttca cgcggccgac gctgttcgag cgccgcacgg attggagcag cacggcggat
       61 ctctcccggc gcatcgacct ggcaccgctg gacaagaccg gcagcaggat gttggccaac
      121 gagttgctca agaagctgcc cgaggtgccc gccgcactca gagagctcgt caccagcagt
      181 gccgaaggca accccttcta catggaagag ctggtgaaga tgctgatcga ccagggtgcc
      241 atccagaccc ggtcagacgg cggcgaggcc tggtcggtca acgccgaacg cttgctcgtg
      301 acccaggtgc cgcccacgct caccggcgtc attcaagcca ggctcgacgg cctgccaccg
      361 gcggagaagc gcgcgctgca gcaagccagc atcgtcggca cggtgttgtg ggaccaggcc
      421 ctggccgcca tcgaagcgca ggcggccgag ctgctgccgg cgctggtgca gcgcgagctg
      481 acgctgccca gaaccgatgc ggcactcgat ggcctgcgcg cgggcctgcg cgagtacgcc
      541 ttccgccacc aggtgctgca ccaggtgacc tacgacacgg tgctcaagcg ccacaagcga
      601 gaaggccacg ccaaggtggc gcagtggctg gccgcactga ccgagcacgg cagcctgcgc
      661 gcgggcgact tcctgggcac cgcggccgaa cacttcgagc aggccggcga cgcgcccaac
      721 gcggccgagt tccatgcccg cgcggccgag caggcggggc agcgcttcgc gcacgaccgc
      781 gtgctggcgc atgtggggcg ggcgctggca ctgctggggg aaacccccgc gccaccgccg
      841 gcacaggccg cgctgcgctg gcggctgctg agtgcgcgcg agcggacgct gaacctgcag
      901 gggcggcgcg acgagcaggc ggccgacctg aacgcgttgg agcagctggc cgacctgctg
      961 gacgacgacc gccggcgcgc ccaagcagcc tggcgccgag gcttccgtgc gctgcgcatg
     1021 gccgactggg cggcgatgga gagcgcggcg cgccacggcc tggcctgtgc cgcgcgggcc
     1081 ggcgatgatg agctgcggct gcaggcgctg cggcttctgg cgtcggcgcg ggtggtgcag
     1141 ggcgacatcg aagccggccg ggcgctggcg cagcagggct tggccgaggc gcgaagcctg
     1201 ggtctgcgcg gcgtcgaggt acggctgctc aacgcgctct cgattgccgc cgagatgcag
     1261 ggcgacctgg tgggctcgct tgacctggac cggcagaacc tactgatctg ccgcgagact
     1321 ggcgaccggc tcaacgaagc catcgccctg tctaaccagg gcgatggctg gctgaagctg
     1381 ggcgacctgg cgcagtcgcg gcgcgatctg gacgcggcgc tgcagatgct gcgcgccaat
     1441 ggcgacagga ccatcgaagg cgcggcgctc gccaacctgt cggccgtggc gctgtggcaa
     1501 ggcgaggaga cgcgggcgct ggcgctggcg cggcaggcgc tggacatcgc ggtggcggca
     1561 aaggcgcgcg attttgaagt gttggccggg ctgcgactgg gcgctgccga actcgcactg
     1621 ggccggaggg ccgccgcgcg ggatgcgtat gcgcaagcgc acgcgcgggc ggcggagatc
     1681 gacagtgcac ggcagcacga cgccatcgct ggcctggcgc gggtggcgct ggccgaaggt
     1741 gacgccgccg cggcgccggc cgcgatggca gcactgaagc cgctgctgga ccacatcgct
     1801 gccggcggca cgctggacgg taccgaagac ccgcgcggga tcgaactcag ctgccacctg
     1861 gccctggccc gcactagcga cccgcgcgct gccgactggc tgatccgcgc ccacggcgcg
     1921 ctgatggccc aggccgacgc gatcagccgc agcaccaccg acgccaggct gcgccagggc
     1981 ttcctgcaga acatcccgca ccaccgcgag atcgtggaac ggttcggagc gtggggcggc
     2041 ttggggtcga aagctgcccc gccgagcgca gttgaccgcc accagcggcc gttcgtgagc
     2101 gttcgagtca tcaatctcta gcgctctggc gcgcgaacga cccctggtgc gatccagtag
     2161 gtcattggtc gcattgctcg ccgaggccca cccttttcat caggcgtgcg ttccaaggcg
     2221 ctcatgtcgg acgcccggcg tcgatcagtg gaagccccat aggagaacga ccgggtccgg
     2281 gcaaactgct cttcaaatcc aaaatacggg gaggaaggct tcatgagagt tgccggattg
     2341 gtcctggcga tggccatgct tgttcttact acaggcgcaa gggctggccc cacactcgaa
     2401 ggcgacaccg tcgatgcggc gatgatccga accatcgaca ccggctacgg actggggcga
     2461 atcgtcggct acggcctgga gggcccgttt gtcgttgtca acggtcccgc agacgtgcag
     2521 cagtacagcg agcattactt gctggacatc gacggcgacc acttcaccgt ggacttcctg
     2581 ggccagttca ccagtggatg gcaagaggga accgtgctga ggctgagcga cctcgacttc
     2641 tctggcgcga gctttctctc cgggctggac atcgacacca acctcgtggg ctacacctta
     2701 gccttcggct cgaactacat cgacatcggc ctgggcggga ccgagttttc cggctcgaca
     2761 tacttcaacg ccaccttcaa ggtgtcgcag gtcacagcaa tcccggagcc cgccacgctg
     2821 gcgctcatgg cggtcggatt gctgggcgtg gcttctgcgg ccaagcgatc acggaggcgg
     2881 cttcgctgag agcgcattcc cgaagcggtc agtggctgga cgagctgcca gccccaagag
     2941 ccgccaaaca gatcttcgcg ccggcggaac cggagtcctc cgaaatgcca ggactggcgc
     3001 tggctctcag ccgggaaggc cctcagcctc cagtgcccgc tgcaccgccg gacgcgccct
     3061 catgcgggcc atgaaggcgt tgagcacctc gaggccggcg atgtcgagct tcagcggcgc
     3121 ggcccatcgt gccaccgtgt agaggtagcc gtcggccacg gtgaagtcgc cgagcacgta
     3181 ctcgcgtatc gccaggtgcc ggtccacata ggccagcctg tcgacaatct tctggcggag
     3241 tggcggcttg gcctcttcgg gcaaggcagg gttgaacagc gggctgaact gttcatggat
     3301 ctcggtgccg atgaacgtga gccactcctg caggcggtag cgctccatcg tgccggccgg
     3361 aggcgcgagc ctcctcgccg gtacctggtc ggcgatccac tgcacgatgg ccggaccttc
     3421 agctagccga gtgccatcgt cgaactccag aagcggcacg tagcccttgg gattgatcgt
     3481 gtagtagtcg gtgccgtcct gcagcttgtg cgacttggtg ctggccagga ccgcctcgaa
     3541 tggcaggcct gcctcatgga gggcgatgtg cggag
//
LOCUS       DASRDG010000309         3961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1079439, whole genome shotgun sequence.
ACCESSION   DASRDG010000309 DASRDG010000000
VERSION     DASRDG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079439"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFA35_05125"
     CDS             <1..223
                     /locus_tag="VFA35_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HZT55587.1"
                     /translation="MYAAQWFAAIRAQHADLDALVARGELAPAFDWLRTNIWTQGSRW
                     PTAELATRASGEALNPAHFRRHLEARYLG"
     gene            complement(247..747)
                     /locus_tag="VFA35_05130"
     CDS             complement(247..747)
                     /locus_tag="VFA35_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZT55588.1"
                     /translation="MVKKIKQTADKQAAASGRLFDSALAGQVKDSAQQIWLAGMGAFS
                     RAQAEGTKVFEALVKEGTNLQKKTTSAAEDRISEVTGKMTSMAGGVQARAGQQWDKLE
                     GIFEERTAKALNKLGVPSAKDVDALTARIDALSAKVAKLSKTTAPARAAAAKAAPAKR
                     AARKSA"
     gene            complement(866..1474)
                     /locus_tag="VFA35_05135"
     CDS             complement(866..1474)
                     /locus_tag="VFA35_05135"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HZT55589.1"
                     /translation="MKLLVKLAPAALAALLAACAASSPAPVLLTLPPVALPVPTAASS
                     GTPAAAPLLAVRRVNIPEYLVARRVRYRADAATLAEWPNTYWAERIEIGVSREFVAAL
                     RAQLPGWSICDTNCGDQVPTLTLQVSLLPMDYLRSGQMLHAHARIALSGAGVPAKLLD
                     TQALSVDIASNADTAQGHAQAITTLIEQVAAAAAPMVRGAKP"
     gene            complement(1471..2457)
                     /locus_tag="VFA35_05140"
     CDS             complement(1471..2457)
                     /locus_tag="VFA35_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZT55590.1"
                     /translation="MKRNALLIGAFVLAALAMMVLGVLWLSGNDLFKKQQEAMIFYRG
                     NVTGLYVGAPVGFRGVAIGQVENIDIQVDRDTLKALVPVRIRLRTAALHFHGTEKGTP
                     VDLPTLVQRGLRARLVAQSFVTGQKSIELDFVPNTPSTLLGDSSKPEIPALAERFGAL
                     IDQVAELPLRDTVQDVRDTVKELRGTLASVQQTLDGTQKVLASAASEFTQAGAASRAT
                     LKAAAEAIRQVQVNSAATLASITQLSDAARQTVLTAQPDLQRTLAGTREAAESARVAM
                     NRVAEMTAPDAALRADLDAALRDLSQAARGLRDWSELLEEKPNAVIFGNKRE"
     gene            complement(2454..3254)
                     /locus_tag="VFA35_05145"
     CDS             complement(2454..3254)
                     /locus_tag="VFA35_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT55591.1"
                     /translation="MTLLAVDDLEMRFGDRLIQTDINFKVEKGTIFAIMGGSGCGKST
                     LLKHLVGLLAPTKGHVRYDGVDYWAADDAGQSKLRAGFGMLFQSAALWSSMTILENVC
                     LPLELQGKLDEAQREARARELLEWVDLGGFADYYPADLSGGMKKRAGLARAIGPQPPL
                     LFLDEPSAGLDPISSKRLDDLILDIRARTGCAVVFVSHELPSLFGIADDGIFLDAESK
                     RPIAHGSPRELRDTCTNPIVHAFLTREVPPPTTPAAQAGPTAVPSATI"
     gene            complement(3256..>3961)
                     /locus_tag="VFA35_05150"
     CDS             complement(3256..>3961)
                     /locus_tag="VFA35_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55592.1"
                     /translation="SAADGLRGKARFRVKDLGRVTADASARAVPIVTIVNLLVGAILA
                     FVGAVQLVKFGAGIYVADLVAIAEAREMAAIITAVVMAGRTGAAFAAELATMQANEEI
                     DALEVLGLSAVDFLVLPRVFALLLMMPLLYVYGCVTGLLGGMVVAAGMLDISPAAYLD
                     RTLDALDWSHLGLGFSKSFVFGALIGMVGCYCGLYAQRNAAGVGIATTNAVVVTIVGI
                     IVLDAVFAICANALGI"
BASE COUNT          551 a         1398 c         1396 g          616 t
ORIGIN      
        1 gatgtacgcc gcgcagtggt tcgccgcgat tcgcgcgcag cacgccgacc tcgacgcgct
       61 ggtcgcgcgc ggcgaactgg cgccggcgtt cgactggctg cgcaccaaca tctggaccca
      121 gggcagccgc tggccgaccg ccgagctcgc gacgcgggcc agcggcgagg cgctgaaccc
      181 ggcgcacttt cggcgccacc tcgaggcgcg ctacctcggg tgacggttcc gccggtcagg
      241 cccggttcag gccgacttgc gcgccgcgcg cttcgccggt gcggccttgg cggcggccgc
      301 ccttgccggc gccgtcgtct tcgacagctt cgcgaccttc gcgctcagcg cgtcgatgcg
      361 cgccgtcagc gcgtcgacgt ccttggccga cggcacgccc agcttgttca gcgccttcgc
      421 ggtgcgttcc tcgaagatgc cctcgagctt gtcccactgc tggccggccc gggcctgcac
      481 gccgccggcc atactggtca tcttgcccgt gacttcgctg atcctgtctt cggcggccga
      541 ggtcgtcttc ttctgcaggt tcgtgccttc cttgaccagc gcctcgaaga ccttggtgcc
      601 ctcggcctgc gcacgcgaga acgcgcccat gccggcgagc cagatctgct gcgcagagtc
      661 cttcacttga ccggccagcg cgctgtcgaa caggcgaccg gacgcggcgg cttgcttgtc
      721 agccgtctgc ttgattttct tgaccatggt gcgaatcctc gttgtgttga tgcgggccga
      781 ctataggcag cggccgtagg gagcgcaccc caattccgtg gagaggcggc tctacttcgg
      841 cgccggccca tcgcgggcca ggctcctagg gtttcgcgcc gcggaccatc ggcgccgccg
      901 cggccgcgac ctgctcgatc agcgtggtga tcgcctgcgc gtggccttgg gcggtgtcgg
      961 cgttcgacgc gatgtcgacg ctcagcgcct gcgtgtcgag cagcttcgcg ggcacgccgg
     1021 ccccgctgag cgcgatgcgc gcgtgcgcgt gcagcatctg gccgctgcgc aggtagtcca
     1081 tcggcagcag gctgacctgc agcgtcagcg tgggcacctg gtcgccgcag ttcgtgtcgc
     1141 agatcgacca gcccggcaac tgcgcgcgca gcgccgcgac gaactcgcgc gacacgccga
     1201 tctcgatgcg ctcggcccag taggtgttcg gccactcggc cagcgtcgct gcatcggcgc
     1261 ggtagcgcac gcgccgcgcg accaggtact cggggatgtt gacgcggcgc accgcgagca
     1321 acggcgcggc ggccggcgtg ccggaactcg cggcggtggg caccggcagc gccaccggcg
     1381 gcagcgtcag cagcaccggc gccggcgacg acgcggcgca cgccgcgagc agggccgcca
     1441 gcgcggccgg cgcgagtttc acgaggagct tcattcgcgt ttgttcccga agatgacggc
     1501 gttcggcttc tcttccagca gttcgctcca gtcgcgcagg ccgcgcgcgg cttgcgagag
     1561 gtcgcgcagg gcggcgtcga ggtcggcgcg cagcgcggcg tcgggcgcgg tcatctcggc
     1621 cacacggttc atcgccacgc gggccgattc ggccgcctcg cgcgtgccgg cgagcgtgcg
     1681 ctgcagatcg ggctgggcgg tcagcacggt ctgccgcgcc gcgtcggaga gctgggtgat
     1741 cgaggccagc gtggccgccg agttgacctg cacctggcgg atcgcctcgg ccgcggcctt
     1801 cagcgtcgcg cgcgaggcgg cgccggcctg cgtgaattcg ctggcggcgc tggcgagcac
     1861 cttctgcgtg ccgtcgagcg tctgctgcac cgaggccagc gtgccgcgca gttccttgac
     1921 cgtgtcgcgc acgtcctgca ccgtgtcgcg cagcggcagc tcggcgacct ggtcgatcag
     1981 cgcgccgaag cgctcggcca gcgccgggat ctcgggcttg ctgctgtcgc cgagcagcgt
     2041 cgacggtgtg ttcggcacga agtcgagctc gatcgacttc tgcccggtca cgaagctctg
     2101 cgccaccagc cgcgcgcgca ggccgcgctg caccagcgtg ggcaggtcga ccggcgtgcc
     2161 cttctcggta ccgtggaagt gcagcgcggc ggtgcgcagc cggatgcgca ccggcaccag
     2221 cgccttcagt gtgtccctgt cgacctggat gtcgatgttc tcgacctggc cgatcgcgac
     2281 gccccgaaaa cccaccggcg cgcccacgta cagcccggtg acgttgcccc tgtagaagat
     2341 catcgcctcc tgctgcttct tgaacaggtc gttgccgctg agccagagca cgcccagcac
     2401 catcatcgcg agcgcggcga ggacgaaagc gccgatcagc agggcgttgc gtttcatatg
     2461 gttgcggatg gtacggcggt tgggccggct tgggcggcgg gcgtcgtcgg cggcggcacc
     2521 tcgcgggtca ggaacgcgtg cacgatcggg ttcgtgcagg tgtcgcgcaa ctcgcgcggg
     2581 ctgccgtgcg cgatcggccg cttgctctcg gcatcgagaa agatgccgtc gtcggcgatc
     2641 ccgaacaggc tcggcagctc gtggctcacg aacaccaccg cgcagccggt gcgcgcgcgg
     2701 atgtcgagga tcaggtcgtc gagccgcttc gagctgatcg ggtcgaggcc ggccgagggt
     2761 tcgtcgagaa acagcagcgg cggctggggc ccgatcgcgc gggcgaggcc ggcgcgcttc
     2821 ttcatgccgc cgctgaggtc ggcggggtag tagtcggcga agccgccgag gtcgacccat
     2881 tcgagcaact cgcgcgcgcg cgcctcgcgc tgcgcttcat cgagtttgcc ttgcagctcg
     2941 agcggcaggc agacgttctc gaggatcgtc atcgacgacc acagcgccgc gctctggaac
     3001 agcatgccga agccggcgcg cagtttcgac tggcctgcgt cgtcggcggc ccagtagtcg
     3061 acgccgtcgt agcgcacgtg gcctttggtt ggcgccagca ggccgaccag gtgcttcagc
     3121 aaggtgctct tgccgcagcc gctgcccccc atgatcgcga agatcgtgcc cttctcgacc
     3181 ttgaagttga tgtcggtctg gatcagccga tcgccgaagc gcatctccag gtcgtcgacc
     3241 gcgagcagcg tcacgtcaga tccccagcgc gttcgcgcag atcgcgaaaa ccgcatccag
     3301 cacgatgatg ccgacgatgg tgacgaccac cgcattggtc gtcgcgatgc cgacgccggc
     3361 cgcgttgcgc tgcgcgtaca ggccgcagta gcagccgacc ataccgatca gcgccccgaa
     3421 tacgaacgac ttcgagaaac ccaggccgag gtgcgaccag tcgagcgcgt ccagggtccg
     3481 gtcgaggtag gcagcgggcg agatgtcgag catgccggcc gcgacgacca tgccgccgag
     3541 cagcccggtg acgcagccgt acacatacag cagcggcatc atcagcagca gcgcgaagac
     3601 gcgcggcagc accaggaagt cgaccgcgct caggcccagc acctcgagcg cgtcgatctc
     3661 ttcgttggcc tgcatcgtcg cgagctcggc cgcgaaggcg gcgccggtgc gcccggccat
     3721 cacgaccgcg gtgatgatcg cggccatctc gcgcgcctcg gcgatcgcga ccagatccgc
     3781 cacatagatg ccggcgccga acttcacgag ctgcaccgcg ccgacgaacg cgaggatcgc
     3841 gcccaccagc aggttgacga tcgtgacgat gggcaccgcg cgcgcgctcg catcggcggt
     3901 gacgcggccg agatccttga cgcggaagcg cgccttgccg cgcaggccgt cggcggccga
     3961 c
//
LOCUS       DASRDG010000310         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_407134, whole genome shotgun sequence.
ACCESSION   DASRDG010000310 DASRDG010000000
VERSION     DASRDG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407134"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="VFA35_05155"
     CDS             <1..1746
                     /locus_tag="VFA35_05155"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT55593.1"
                     /translation="GRWVRGEHYAEITIPPTIQALLEARLDRLAHEERITVEPAAVIG
                     MQFALPAVAALSPEAARAHVGERLASLAHKQFIQPTESTEGDVLYRFHHHLVRETVYG
                     GLLKRTRASLHIDFVRWADRINAERGRALEFEEILGYHLEQAHRYLGELGPLDAAAVA
                     VGADAARRLAGAARRAFARGDMHAAANLYRRAAALLPDADANRLALLPELGETLLELG
                     DFDDARKVLDEAALAAEQAGNQRVRASARIVRMLVRMYSAEPGDWSSAALQVAEETIP
                     ALEREGAHDELANAWRLSGRVHGIAARFGQVVNAIEKSITHARLAGDARMVARAGMAL
                     SSTALYGPMPVPQAIAQCERIIAEGLTDRQVEAIVMCTLAQLHAMNAEFETARGLYRR
                     GRALLRELGQGVNAASTGLGVGRVEWLAGDLATAERELRGDLDFLAQRGETYFLSTMA
                     AQLSCIVRDLGDADAALALSKTAEEAATEDDTVSQILWRVARATLVARRGDLAEAEAL
                     ARRAVELSRSTDAPIFQADALAELAAVLGLGGRVNDALATLDAALGLYRDKGDRASAA
                     RAAALAERLRSLPPT"
     gene            complement(1775..2722)
                     /locus_tag="VFA35_05160"
     CDS             complement(1775..2722)
                     /locus_tag="VFA35_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55594.1"
                     /translation="MSITNNSGNPTTHVQFSGGTDVSGDLTQANGNAGATATYVETIP
                     KSACTPTTPGGTTIQCDPGKMNTGANKSFVVIFAAPSLAAADHWASDAKINFTWTFDY
                     GSGNTSGTPSSLLCNGVVASPPPCIETSSTGLVTTATNEILEKFLTYIPSFGGTFWTG
                     NGSSVLGPDGSNLRPTALTKLKVPTGLNLTTAQVTLTVASGAVSGNTTTTNTAVVEVP
                     NGGNLFGSFVTIELRRDASTILKGAKIANAVVSYSHDDPNALNPVQPCPTGGIPTIAQ
                     PVCLFGSPIEFTKKNAPTPDDVGDWLFVIHALENGVLNW"
BASE COUNT          400 a          947 c         1003 g          429 t
ORIGIN      
        1 gggcgctggg tgcgcggcga acactacgcc gagatcacga tcccgccgac gatccaggcc
       61 ctgctcgaag cgcggctcga tcggctcgcg cacgaagagc ggatcaccgt cgagccggcc
      121 gccgtgatcg gcatgcagtt cgcgttgccc gcggtggcgg cgctgtcgcc cgaggcggcg
      181 cgcgcccacg tcggcgagcg cctggcgagc ttggcccaca agcagttcat ccagccgacc
      241 gagtcgaccg agggcgacgt gctctaccgc ttccaccacc acctggttcg ggagacggtc
      301 tacggcggcc tgctcaagcg cacgcgcgcg agcctgcaca tcgacttcgt gcgctgggcc
      361 gaccgcatca acgccgagcg cgggcgcgcg ctcgagttcg aggagatcct gggctaccac
      421 ctcgagcagg cgcaccgcta cctgggcgag ttggggccac tcgacgccgc cgcggtcgcg
      481 gtcggcgccg atgccgcgcg gcgcctggcc ggcgcggcgc ggcgcgcctt cgcgcgcggc
      541 gacatgcatg ccgcggcgaa cctgtaccgg cgcgcggcag cgctgctgcc ggacgccgat
      601 gcgaaccggc tcgcactgct gcccgagctg ggcgagacct tgctggagct cggcgacttc
      661 gacgatgcgc gcaaggtgct cgacgaagcg gcgctcgccg ccgagcaggc cggcaaccag
      721 cgcgttcggg cgtcggcgcg catcgtgcgg atgctggtgc gcatgtacag cgccgagccc
      781 ggcgactgga gttcggcggc cctgcaggtc gccgaagaaa ccatccccgc gctggaacgc
      841 gaaggcgcac acgacgagct ggcgaacgcc tggcggctca gcggtcgggt ccatggcatc
      901 gccgcgcgct tcgggcaggt cgtgaacgcg atcgaaaagt cgatcacgca cgcgcgcctg
      961 gccggcgatg cccggatggt cgcgcgcgcc gggatggcgc tgtcgagcac ggcgctgtac
     1021 gggccgatgc cggtgccgca agcgatcgcg cagtgcgagc gcatcatcgc cgagggcctg
     1081 accgatcggc aggtcgaggc catcgtgatg tgcacgctgg cgcagttgca tgcgatgaac
     1141 gccgagttcg agacggcgcg cggcctgtat cgccgcgggc gcgcgctgct gcgcgagttg
     1201 ggccagggcg tgaacgcggc atcgaccggg ctgggcgtcg ggcgtgtcga gtggctcgcg
     1261 ggcgatctgg ccacggccga acgcgaactg cgcggcgacc tggactttct cgcgcaacgc
     1321 ggcgagacct actttctctc gacgatggcg gcgcaactct cctgcatcgt gcgggacctg
     1381 ggcgacgcgg acgcggcgct ggcgctctcg aagaccgccg aggaggccgc caccgaagac
     1441 gacaccgtct cgcagattct ttggcgcgtg gcgcgcgcaa cgctggtggc gcgccgcggc
     1501 gacctggccg aggctgaggc gctggcgcgc cgggcagtcg agctgtcgcg cagcaccgac
     1561 gcgccgatct tccaggccga cgcgctcgcc gaactcgccg cggtgctggg cctgggcggg
     1621 cgcgtcaacg acgcgctcgc gacgctcgac gcggcgctcg ggctctaccg cgacaagggc
     1681 gaccgcgcct cggccgcgcg cgccgccgcg ctggccgagc ggctcaggtc attgccgccg
     1741 acctgaacca cgccggcggc gccacgcggc cgcatcacca gttcaagacg ccgttttcga
     1801 gcgcatggat gacgaacagc cagtcgccga catcgtcggg cgttggcgcg ttcttcttgg
     1861 tgaattcgat cggcgagccg aacaggcaca ctggctgcgc aatggtcggg atgccgcccg
     1921 tcggacacgg ctgcaccggg ttcagcgcat tggggtcgtc gtgcgagtac gagaccacgg
     1981 cattggcgat cttcgcaccc ttcagaatcg tgctggcatc gcgccgcaac tcgatggtca
     2041 cgaagctgcc gaacaagttc ccgccgttcg gcacctcgac gaccgccgtg ttcgtcgtgg
     2101 tcgtgttgcc ggagactgcg ccggacgcca ccgtcaaggt cacctgcgcc gtggtcagat
     2161 tcagtccggt cggcaccttg agcttggtca gcgccgtcgg gcgcagattg cttccgtccg
     2221 gtcccaacac cgacgagccg ttgccggtcc agaacgttcc gccgaagctc gggatgtagg
     2281 tcaggaactt ttcgaggatc tcattggtcg cggtcgtgac cagccccgtc gaactggttt
     2341 cgatgcaggg cggcgggctg gccacgacgc cgttgcagag cagggacgac ggcgtacccg
     2401 aggtgttgcc gctcccatag tcgaaggtcc acgtgaagtt gatcttcgcg tcactcgccc
     2461 agtggtcggc ggcagcgagg ctgggcgccg cgaagatcac cacgaacgac ttgttggcgc
     2521 ccgtgttcat cttccctggg tcgcactgga tcgtcgtgcc gccgggcgtc gtgggcgtgc
     2581 aggctgattt cgggatcgtc tcgacgtaag tcgcggtcgc gccggcgttg ccattcgcct
     2641 gcgtcaagtc gccgctcaca tccgtgccgc cgctgaactg gacatgggtg gtcgggttcc
     2701 cagagttgtt ggtgatgctc accgtgtagg ccgcgtaggt ggtcagcccg gggcggctca
     2761 ccgacactgt ttctgggat
//
LOCUS       DASRDG010000311         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_407151, whole genome shotgun sequence.
ACCESSION   DASRDG010000311 DASRDG010000000
VERSION     DASRDG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407151"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..1345
                     /locus_tag="VFA35_05165"
     CDS             416..1345
                     /locus_tag="VFA35_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZT55595.1"
                     /translation="METKWFEDFVSLAETRSFSRSAQLRHVTQPAFSRRIQSLEAWAG
                     IDLVDRSSYPTRLTPAGETLHAQALEILGALQATRNMMRGHKAGGNDMIEFAVPHSLA
                     FTFFPHWVMDLRKRFGAMKSRLIALNVHDAVLHLVEGSCDLLLAYHHPSQPLQLNPDR
                     YEMLSLGRETLVAYARGDADGQPMFRLPAAPGRSVPYLSYAPGAYLGQLVEVIAKQAS
                     EPLHLEPIYETDMAEGLKAMAIEGHGLAFLPATSVRKELKSKRLVRAAPAGMHELQMD
                     IRIYRERPEMARRSKPGALALWAFLANPAGAHE"
     gene            complement(1361..2863)
                     /locus_tag="VFA35_05170"
     CDS             complement(1361..2863)
                     /locus_tag="VFA35_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZT55596.1"
                     /translation="MEPINIAPVQATASLPDFVLRQFDQASPQPDHRQLYGILQRGIR
                     DAVLPAGLMLPPTRQLCEALGIARNTVVHVYEQLALEGYVQAGIGRGTFVAAVAPRLV
                     AERAAPPASPSAARGALLSRRGGHTIGAAGAARVQWGAFTPGVPEVRLFPAQIWNRLH
                     ARLWRSASPEQLTYATGAGDAGLRESVAEYLQGTRGVVCTPEQVVITSGTQQSLYLVQ
                     QLLADPGDAVWLEDPGYWGARSVFSAGALKLVPVGVDAEGLAPSPEQLRRPPRLMFVS
                     PSHQYPTGVLMSYGRRRQLLDYAAAHGVWVVEDDYDSEFRYGARPLPALQGLDRHGRV
                     IYLGTFSKTLFPALRLAYLVLPPDLVEPFARALNELFREGQTLQQAVLARFLAEGHYA
                     THIRRMRAVYGARHDALVQAIARHFGDHLPVIGGDAGLHLVLGLPPQVDDDAVAQQAQ
                     RAGVTTRPLSLYHMRRPAAAKGLLLGYGAVREEEVAPSFAKLAKVLQRSL"
BASE COUNT          457 a         1077 c         1072 g          437 t
ORIGIN      
        1 ggacccgata tcccgagggg ccgctcgtca aaaccagcaa tctccctgtc ttggcgtccc
       61 atcgacccat gtcggtgacg agaacgcggc cgtcctgcaa aggcagcact ccgcgcggcg
      121 caccgaggcg ctgaccagcc aatccaacgc agaacccttc cgccacccgc actttgactg
      181 caggcacgcc ggcgcatgtg ccttcagtgg cataggcgcc ccccgatgcc gtggcggccg
      241 aggtggcgca gaacagtgtg gaggcgacaa tgaatatcag gactggcgaa gtctgacgag
      301 ccggtggcat gaatgatcgc tggtgacgtc gatgccggca ctgtaccggc tggcgctgcc
      361 gccacttcgt gggcttctgc gatcgcccgg ccggcgcctc gctacactgc ccggcgtgga
      421 aaccaagtgg ttcgaggact tcgtcagcct ggccgagacg cgcagcttct cgcgttcggc
      481 gcagttgcgg cacgtgacgc agccggcgtt ctcgcgccgc atccagtcgc tcgaggcctg
      541 ggccggcatc gacctggtcg accgctcgtc gtacccgacg cgcctgaccc cggccggcga
      601 gacgctgcat gcgcaggcgc tcgagatcct gggcgcgctg caggccaccc gcaacatgat
      661 gcgcggccac aaggcgggcg gcaacgacat gatcgagttc gcggtgccgc attcgctcgc
      721 gttcacgttc ttcccgcact gggtcatgga tctgcgcaag cgcttcggcg cgatgaagag
      781 ccggctgatc gcgctgaacg tgcacgacgc ggtgctgcac ctggtcgaag gcagctgcga
      841 tctgctgctt gcgtaccacc acccctcgca gcccctgcag ctcaaccccg atcgctacga
      901 gatgctgagc ctgggccgtg agacgctcgt cgcctacgcc cgcggtgacg ccgacggcca
      961 gccgatgttc cgcctgcccg cggcccccgg gcgcagcgtg ccctacctct cgtatgcgcc
     1021 gggcgcctac ctcggtcagc tggtcgaggt catcgccaag caggcgagcg agccgttgca
     1081 cctggaaccg atctacgaga ccgacatggc cgagggcctg aaggcgatgg cgatcgaggg
     1141 ccacggcctg gccttcctgc ccgccacctc ggtgcgcaag gagctgaaga gcaagcgcct
     1201 ggtgcgcgcc gcgcccgccg gcatgcacga gctgcagatg gacatccgca tctaccgcga
     1261 acgccccgag atggcgcgcc gcagcaagcc cggtgcgctc gcgctgtggg cgtttctggc
     1321 caacccggcc ggcgctcacg agtagccctc acaagcagcc tcacaagctg cgttgcagca
     1381 ccttcgccag cttcgcgaag ctcggcgcga cctcttcctc gcgcaccgcg ccgtagccga
     1441 gcagcaggcc cttggccgcg gccggccggc gcatgtggta cagcgagagc gggcgggtcg
     1501 tcacgccggc gcgctgggcc tgctgcgcga ccgcgtcgtc gtcgacctgc ggcggtagcc
     1561 cgagcaccag gtgcaggccg gcatcgccgc cgatcaccgg caggtggtcg ccgaaatgcc
     1621 gcgcgatcgc ctgcaccagc gcgtcgtggc gggcgccgta gaccgcgcgc atgcgccgga
     1681 tgtgggtcgc gtaatggccc tcggcgagga agcgcgccag caccgcctgc tgcagggtct
     1741 ggccctcgcg gaacagttcg ttcagcgcgc gcgcgaaggg ctcgaccaga tcgggcggga
     1801 gcaccaggta ggccaggcgc agcgccggga acagcgtctt cgagaaggtg ccgaggtaga
     1861 tcacgcggcc gtgccggtcg agcccctgca gcgccggcag tggccgggcg ccgtagcgga
     1921 actcgctgtc gtaatcgtcc tcgacgaccc agacgccgtg cgccgcggcg tagtcgagca
     1981 gctggcggcg ccggccgtag ctcatcagca cgccggtcgg gtactggtgc gagggcgaga
     2041 cgaacatcag ccgcggcggc cggcgcaact gctcgggcga gggcgccagc ccttcggcgt
     2101 cgacgccgac cggcaccagc ttcagcgcgc cggcgctgaa cacgctgcgt gcgccccagt
     2161 agcccgggtc ttcgagccag accgcgtcgc ccgggtcggc gagcagctgc tgcaccaggt
     2221 acagcgactg ctgggtgccg ctggtgatca ccacctgctc cggggtgcag accacgccgc
     2281 gcgtgccttg caggtactcg gcgaccgact cgcgcagccc agcatcgcca gcgccggtgg
     2341 cgtaggtcag ctgctcgggg ctggcactgc gccacagtcg cgcgtgcagg cggttccaga
     2401 tctgcgccgg gaacagccgc acttccggaa cgccgggcgt gaatgcgccc cactggaccc
     2461 gcgccgcccc ggcggcaccg atggtgtgcc cgccgcgccg cgacagcagc gcgccgcgcg
     2521 ctgcgctggg cgatgccggc ggggccgcgc gctcggcgac caggcgcggc gcgaccgcgg
     2581 cgacgaaggt gccgcgaccg atgcccgcct gcacatagcc ttcgagcgcg agctgttcgt
     2641 agacgtggac gacggtgttg cgcgcgatgc ccagcgcctc gcacagctgg cgcgtcggcg
     2701 gcagcatcag gcccgccggc aggaccgcgt cgcggatgcc gcgctgcagg atgccgtaga
     2761 gctggcggtg gtcgggttgt gggctggcct ggtcgaactg gcgcagcacg aagtcgggca
     2821 gcgaggccgt ggcctgcacc ggggcgatgt tgattggttc catggcgcgt atcttgattg
     2881 gctctggaag cgggatcaat agcgaactag actgcgccgc attcctctgc acggagacat
     2941 gacgcgatga atgcaccact tcgcttttat gacctgggcg ccgcgctgct ggccctgctg
     3001 gttctcggcg gctgcggcgg cggcgacgat tcggcggcag aca
//
LOCUS       DASRDG010000312         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_502303, whole genome shotgun sequence.
ACCESSION   DASRDG010000312 DASRDG010000000
VERSION     DASRDG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502303"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFA35_05175"
     CDS             complement(<1..194)
                     /locus_tag="VFA35_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55597.1"
                     /translation="MAERTQAVLGAAVSLGTRFAYKRRMALDSTLILTALVKRYGDTR
                     AVDGIDLTLQRGEVLALVGES"
     gene            292..996
                     /locus_tag="VFA35_05180"
     CDS             292..996
                     /locus_tag="VFA35_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZT55598.1"
                     /translation="MQIRVINPNTTARMTALIDAAARAAAAPGTEITAVNPSFGAPSI
                     ECHHDDVWAAAGVAEQVRLGEADGGVDAYVIACFGDPGLHAARELARGPVIGIAEAAF
                     HAASLVATGFSVVTTLTRTCVIAEHLVLQYGFERRCRGIHGTDLAVLELDDPASGAYA
                     RILACAQQALAHDRSGAIVLGCAGMAELCHRLTAELGVPVIDGVAAAVKFAEALVALS
                     LRTSKHGDYAWPPAKA"
     gene            1063..1530
                     /locus_tag="VFA35_05185"
     CDS             1063..1530
                     /locus_tag="VFA35_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55599.1"
                     /translation="MNHRQSPLGPLALLLAALATPAFGASSTASLASDSASSAASSGS
                     DSLKKSSDSSSKTTTAAAGDYEVMAVTAAAERPGTLRLTLQAVADRSAQGEYFLYVPQ
                     QTVDRGRLAPGQIVTARARPYGLEFAKGDPGQAFFLVIDDDWAADLPSHPISL"
     gene            1527..2447
                     /locus_tag="VFA35_05190"
     CDS             1527..2447
                     /locus_tag="VFA35_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HZT55600.1"
                     /translation="MTGCERSARALLLAALGSIACGAGHAGALQFCDHPAALNAAQKD
                     TLLRFGAIIKTELENSGARLALVARSGLDLSRFGLRYSHAGFSLKASPNTPWSVRQLY
                     YACDEGRPRIFDQGLAGFLLGTDDPAIGYVSVVLMPAAQAGELERAALNNARALQLLN
                     AAYSANAYAWGLQYQNCNQWVVEMLASAWGRIDDDADAPRAQAQRWLAAHDYAPSRVE
                     VGNRALIWASAFVPWLHSDDHPRGDLAQRVYRVSMPAAIESFVRSTLPAASRIEFCHT
                     DRHVVVHRGWEPIAEGCTPGAQDTVIPLAD"
     gene            complement(2453..>3212)
                     /locus_tag="VFA35_05195"
     CDS             complement(2453..>3212)
                     /locus_tag="VFA35_05195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HZT55601.1"
                     /translation="ATFRHPQADREVELHGHIVGYAFKRARRRSIGFIVGTEGLSVNA
                     PKWVGLHDIEAALREKSRWILKKLHEQRERAQRMQSAKVEWRDGTSIPFLGEPVVVAL
                     DARATGAVLDTAADALPGVPRLTLRIGLPPGAAPEQIRDAVQSWLQRQARRAFEARCA
                     HFAPQLKVRVTKLSLSSAQTRWGSASADGSIRLNWRLIHFAMSSIDYVVAHELAHLRH
                     MDHSPRFWDVVRSVLPDYEQARISLKDGVVPVFD"
BASE COUNT          471 a         1201 c         1096 g          444 t
ORIGIN      
        1 gactcgccga cgagcgcgag cacctcgccg cgctggagcg tcaggtcgat gccgtcgacc
       61 gcccgggtgt cgccatagcg cttgaccagc gcggtgagga tgagggtcga gtcgagggcc
      121 atgcggcgct tgtatgcaaa gcgtgtgcca agcgacacag cggcgccgag caccgcttgt
      181 gtgcgttcgg ccacgtcgca tcaagtgttt gctcatgatt ggggcggtcc tcgccttaaa
      241 acgggcgcac gacttgctac gatctccggc cacgctttgt atacaaacac catgcagatc
      301 cgcgtcatca accccaacac caccgcccgc atgacggcgc tgatcgacgc cgcggcgcgc
      361 gcggccgcgg cgcccggcac cgagatcacc gccgtgaacc cgagcttcgg cgcacccagc
      421 atcgaatgcc accacgacga cgtgtgggcc gccgccggcg tggccgagca ggtgcgcctg
      481 ggcgaggcgg acggcggcgt cgacgcctac gtcatcgcct gcttcggcga cccgggcctg
      541 cacgccgcgc gcgagctcgc gcgcggcccg gtgatcggca tcgccgaggc cgcgttccac
      601 gccgcgagcc tggtcgcgac cggcttctcg gtcgtcacca cgctgacgcg cacctgcgtg
      661 atcgccgagc acctggtgct gcagtacggc ttcgagcgcc gctgccgcgg catccacggc
      721 accgacctcg cggtgctcga actcgacgac ccggccagcg gcgcctacgc gcgcatcctg
      781 gcgtgcgcgc agcaggccct ggcgcatgac cgcagcggcg cgatcgtgct cggctgcgcc
      841 ggcatggccg agctgtgcca ccggctcacg gccgagctcg gcgtgccggt gatcgacggc
      901 gtggcggcag cggtcaagtt cgccgaggcg ctggtggcct taagcctgcg cacctccaag
      961 cacggcgact acgcgtggcc gccggcgaag gcctgaacgc aggcctttga tagcatgcgc
     1021 cgacccgcgg caccaaccgc tgcaccaacc catcggagcg ccatgaacca ccgccagtcc
     1081 ccgctcggcc ccctcgcgct gctgctcgcg gcgctcgcca cgccggcgtt tggcgccagc
     1141 tccaccgcgt cgctcgcgtc cgacagcgca tcgtccgcgg cgagcagcgg ttcggactcg
     1201 ctgaagaagt cgagcgacag ctcctcgaag acgacgacgg ccgccgccgg cgactacgag
     1261 gtgatggccg tcaccgccgc ggccgagcgc cccggcacgc tgcggctgac gctgcaggcc
     1321 gtggccgacc gcagcgcgca gggcgagtac tttctctacg tgccgcaaca gaccgtcgac
     1381 cgcggccgcc tcgcccccgg ccagatcgtg accgcgcgcg cgcggcccta cggcctcgag
     1441 ttcgccaagg gcgacccggg ccaggccttc ttcctcgtga tcgacgacga ctgggccgcc
     1501 gacctgccct cgcatcccat ctcgctttga cgggctgcga gcgcagcgcc cgcgcgctgc
     1561 tgctggccgc gctgggcagc atcgcgtgcg gcgcgggtca cgcgggcgcg ctgcagttct
     1621 gcgaccaccc ggcagcactg aacgcggcgc agaaagacac gctgctgcgc ttcggcgcga
     1681 tcatcaagac cgagctcgag aactccggcg cgcggctggc gctggtggcg cgctcgggct
     1741 tggacctgag ccgcttcggc ctgcgctact cgcacgccgg cttcagcctg aaggcgagcc
     1801 cgaacacgcc gtggtcggtg cgccagctct actacgcctg cgacgagggg cggccacgga
     1861 tcttcgacca gggcctggcg ggcttcctgc tcggcaccga cgacccggcc atcggctacg
     1921 tctcggtcgt gctgatgccg gcggcgcaag ccggcgagct cgaacgtgcc gcgctcaaca
     1981 acgctcgcgc gctgcagctg ctcaacgcgg cctacagcgc gaacgcctat gcctggggcc
     2041 tgcaatacca gaactgcaac cagtgggtcg tcgagatgct cgccagcgcc tggggccgga
     2101 tcgacgacga cgccgatgcg ccgcgcgcgc aggcgcagcg ctggctcgcc gcgcacgact
     2161 acgcgcccag ccgcgtcgag gtcggcaacc gcgcgctgat ctgggccagc gccttcgtgc
     2221 cctggctgca cagcgacgac cacccgcgcg gcgacctcgc gcagcgcgtc taccgcgtca
     2281 gcatgccggc cgccatcgaa agcttcgtgc gcagcacgct gcccgcagcg agccgcatcg
     2341 agttctgcca caccgaccgg cacgtcgtcg tgcaccgtgg ctgggagccg atcgccgaag
     2401 gttgcacgcc gggcgcgcag gacacggtga tcccgctggc cgattgatca gctcaatcga
     2461 acaccggcac gacaccgtcc ttcaagctga tgcgcgcctg ctcgtagtcg ggtagcaccg
     2521 agcgcaccac gtcccagaag cgcgggctgt ggtccatgtg gcgcaggtgc gcgagttcgt
     2581 gcgcgacgac gtaatcgatg ctgctcatcg cgaaatggat caggcgccag ttcaggcgga
     2641 tcgagccgtc ggcactcgcg ctgccccagc gggtctgcgc cgacgacagg ctgagcttcg
     2701 tcacgcgcac cttcagctgc ggcgcgaagt gcgcgcagcg cgcctcgaac gcgcgccgcg
     2761 cctggcgctg cagccagctc tgcacggcgt cgcggatctg ctcgggtgcg gcgccgggcg
     2821 gcaggccgat gcgcagggtc agccgcggca cgccgggcag cgcgtcggcc gcggtgtcga
     2881 gcacggcgcc ggtggcacgc gcatcgagtg ccacgaccac gggctcgccg aggaacggaa
     2941 tcgacgtgcc gtcgcgccac tcgaccttgg ccgactgcat gcgctgcgcg cgctcgcgct
     3001 gctcatgcag cttcttcagg atccagcgcg acttctcgcg cagcgcggct tcgatgtcgt
     3061 gcagcccgac ccacttcggc gcgttcacgc tcaagccctc ggtaccgacg atgaagccga
     3121 tgctgcgccg gcgcgcgcgc ttgaacgcat agccgacgat gtgaccgtgc agctccacct
     3181 cgcgatccgc ctgcggatgg cggaaggtgg cc
//
LOCUS       DASRDG010000313         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_407544, whole genome shotgun sequence.
ACCESSION   DASRDG010000313 DASRDG010000000
VERSION     DASRDG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407544"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1688
                     /locus_tag="VFA35_05200"
     CDS             51..1688
                     /locus_tag="VFA35_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55602.1"
                     /translation="MVKTAARTQRPSAPSTRRPRIGVFSGYDPRPWVMRDCADQDIRT
                     LEALIARLEQSGAFELVFPGRNKKGWDKLCYTTELCEEYAAAFAQAGVDGLLNVHPTW
                     TFPQTSQKVVAAYAQKLKSKDATARPRLCMVSIQDTTVPGMVSGMATGGSFNQNGEAF
                     VHVYGDFDDDGTLAELHTALQMFGRRAGVQDKVRKVVDSLHKLHALEFGSFSLQMPTT
                     RINQEELTKRWGITSESLDQQVFLDRAFAMFEWTGEPGLSPISKTKDKRVGEAVAKNF
                     DTHPDKFTAIKGRTVTRDKYALQVAMYFATSDIAREKGAGAVTIKCQDECSGKYATCC
                     MATSFLGNDVDPLGNKKPMIPTSCETDLPTMYSQFLLKELADKPAGFGDFRYVKTDTK
                     GRTLLAIVNCGQHPVYYAGRESDSTAQKFAATDHPGQEHFYAAGGASVRMRTAGGQEV
                     TVARLGVENGRLYLVATLMQTTDVPESRHAKYNPSWPIIEGLVPVTDKVLGRRWPSNH
                     LGFVYGDHLAALIELAERMGIGYWIWDRDGTEYWKPS"
     gene            complement(1709..>2098)
                     /locus_tag="VFA35_05205"
     CDS             complement(1709..>2098)
                     /locus_tag="VFA35_05205"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HZT55603.1"
                     /translation="FLAGLRGPGDLDHWPGRGRSFEGLVVEELIQRLSLHARRPYFAF
                     WRTQAGAEVDLLFDLGRGLVPVEIKLGPVDRHGVQGLRHCMADLRLPRGYVVTASVDE
                     PWDMGNGIRVLPWRDLARGNLPPQWLR"
BASE COUNT          372 a          717 c          727 g          282 t
ORIGIN      
        1 cgcgctgccg gtcgcgcgcg caggccgcct ttgcgtagga tgcgttgccc atggtcaaga
       61 ccgccgcccg cacgcagcgc ccctccgctc cttcgacgcg acgcccgcgc atcggcgtct
      121 tctccggcta cgacccgcgg ccgtgggtga tgcgcgactg cgccgaccag gacatcagga
      181 cgctcgaggc gttgatcgcg cggctcgagc agagcggcgc gttcgagctc gtctttcccg
      241 gccgcaacaa gaaggggtgg gacaagctct gctacaccac ggagctctgc gaggagtacg
      301 cggccgcgtt cgcccaggcc ggcgtcgacg ggttgttgaa cgtgcacccg acctggacct
      361 ttccgcagac gagccagaag gtcgtcgcgg cctacgcaca gaagctgaag agcaaggacg
      421 cgaccgccag gccgcggctg tgcatggtgt cgatccagga cacgacggtg cccggcatgg
      481 tgagcggcat ggcgaccggc ggctcgttca accagaacgg cgaggcgttc gtgcacgtct
      541 acggcgactt cgacgacgac ggcaccctcg ccgagctgca cacagcgctg cagatgttcg
      601 gccgccgcgc cggcgtgcag gacaaggtgc gcaaggttgt cgactcgctg cacaagctgc
      661 acgcgctcga gttcggcagc ttctcgctgc agatgccgac cacgcgcatc aaccaggagg
      721 agctgaccaa gcgctggggc atcaccagcg aaagcctcga ccagcaggtg ttcctcgacc
      781 gcgcgttcgc gatgttcgag tggaccggcg agccgggcct gtcgccgatc agcaagacga
      841 aggacaagcg ggtcggcgaa gcggtggcga agaacttcga cacgcacccc gacaagttca
      901 ccgccatcaa ggggcgcacc gtgacgcgcg acaagtacgc gctgcaggtg gcgatgtact
      961 tcgcgacctc cgacatcgcg cgcgagaagg gcgccggcgc ggtgacgatc aagtgccagg
     1021 acgaatgctc gggcaagtac gcgacctgct gcatggcgac ctcgttcctc ggcaacgacg
     1081 tcgacccgct cggcaacaag aagccgatga tcccgaccag ctgcgagacc gacctgccga
     1141 cgatgtactc gcagttcctg ttgaaggagc tcgccgacaa gccggcgggc ttcggcgact
     1201 tccgctacgt gaagaccgac acgaagggcc ggacgctgct ggcgatcgtc aactgcggcc
     1261 agcatccggt ctactacgcg ggcagggaga gcgactcgac ggcgcagaag ttcgcggcca
     1321 ccgaccatcc gggccaggag cacttctacg cggccggcgg cgcgtcggtg cgcatgcgca
     1381 ccgcgggcgg ccaggaggtc acggtggcca ggctgggcgt ggagaacggc cgcctctacc
     1441 tcgtcgcgac gctgatgcag accaccgacg tgcccgagtc gcgccatgcg aagtacaacc
     1501 cgagctggcc gatcatcgag ggcctggtgc cggtcaccga caaggtgctc ggcaggcgct
     1561 ggccgagcaa ccacctcggc ttcgtctacg gcgaccacct cgcggcgctg atcgagctcg
     1621 ccgagcgcat gggcatcggc tactggatct gggaccgcga cggcaccgag tactggaagc
     1681 cgagctgaat cgcgcgcaac cgtgatgttc accgcagcca ttgcgggggc aggttgccgc
     1741 gcgcgaggtc ccgccacggc agcacgcgga tgccgttgcc catgtcccac ggttcgtcga
     1801 ccgacgcggt caccacgtag ccgcgcggca atcgcaggtc ggccatgcaa tggcggagcc
     1861 cctggacgcc gtggcggtcg accgggccga gcttgatctc gaccggcacg aggccgcggc
     1921 cgagatcgaa cagcaggtca acctcggcgc cggcctgcgt gcgccagaac gcgaagtagg
     1981 gccgccgggc gtgcaggctc agccgctgga tcagttcctc gaccacgagt ccctcgaacg
     2041 agcggccgcg tccgggccag tggtcgaggt cgccgggccc gcgcaggccg gcgaggaa
//
LOCUS       DASRDG010000314         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1080378, whole genome shotgun sequence.
ACCESSION   DASRDG010000314 DASRDG010000000
VERSION     DASRDG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080378"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2584)
                     /locus_tag="VFA35_05210"
     CDS             complement(<1..2584)
                     /locus_tag="VFA35_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZT55604.1"
                     /translation="MPIAILRPPRLVGRERERAQLAAALASVRSTLVGGEPGIGKTRL
                     LEDHAARVDGAIVVPARPGDARLLYAVMARALRALAARFGEPQPGWLRGELARVLPEL
                     GTANPGRLDAGAFQRAVAESLSAWARAGLSLVVLDDAQFADEATLAALPALCGGTGAP
                     IAWCIGCRAGDVPTLLDEWRHAGDRDALAEIRLGPLDPAAVETLLEALEIPGLDARRW
                     AQPLWCHTGGNPLFVLETLRALIAGGTQLLEALPEDLPTPHDVGALIARRLEQLSPGA
                     LALARVIAIAGLDFSIELAAHVVGRPVVELADAWRELESGHVIRDCGFAHDLLYEVVL
                     RSVPEAIAQALHRAVAEFLQSAGKPAARIASHWREAQEWAAAATAFETAADVALRHSR
                     REDELSLIEQAIDCAGRGGDAARAFALRARAVEPMLIVRTIDAALALTDRLMVDATTD
                     EQRLQAQLARASTLLMASRFVDAAEVAGGARTAARRLRQSRPELDAVRLEALALANSG
                     RASQAVEMLHKVESRFEKSRDLLLRYKFATDYGHALSQAARWPEAIRLISRAVTLAEE
                     LGDVAETIVNLTNLAGALGYVGRMAGAVAQAERARALRDRMGIAVGAPMAHNDTVLGM
                     LYVALGRYREALQAFELAGEHFRAGGAPTWIAVNENHRALALVHLGQEARALQLLARD
                     DGLPRSTRARRWTIHARAEQARGRPGAMQLRAALELLGAEGSPPLRMGAQLDLARELA
                     PDEGVALCRQTRAEATRLGLLALAQSARIREVDGLVRLGDSVAAAALAQTVLAQLEAC
                     HPSDLYLAEAWSTAYRALDSDPATRGAGRETLRRGQAWVREVAAHHVPEEFRDSFLHR
                     NPVNR"
BASE COUNT          343 a          953 c         1001 g          310 t
ORIGIN      
        1 accggttgac cggattgcga tgcaggaagc tgtcgcgaaa ctcctcgggc acgtggtgcg
       61 cggcgacttc gcggacccat gcctggccgc ggcgcaacgt ctcgcgcccc gcgccgcgcg
      121 tggccgggtc cgagtcgagc gcgcggtatg ccgtcgacca cgcctcggcg aggtacaggt
      181 ccgacgggtg gcacgcttcg agctgcgcga gcacggtctg cgcgagcgcg gcggcagcca
      241 cgctgtcgcc gaggcgcacg aggccatcga cctcgcggat gcgcgcgctt tgcgccagcg
      301 ccagcaggcc gagccgggtg gcctcggcgc gcgtctgccg acacagtgcc acgccctcat
      361 cgggggccag ctcgcgcgcc aggtcgagct gggcacccat gcgcagcggc gggctgcctt
      421 cggcacccag cagctcgagc gcggcccgga gctgcatcgc gcccgggcgc ccacgcgcct
      481 gctcggcgcg cgcatggatg gtccagcgtc gcgcccgcgt cgaccgtggc aggccatcgt
      541 cgcgcgccag cagctgcagc gcccgggcct cctggccgag gtgcacgagg gcgagcgcgc
      601 ggtggttctc gttgaccgcg atccacgtcg gggcgccgcc ggcgcggaag tgctcgcccg
      661 cgagctcgaa cgcctgcagg gcctcgcggt agcgaccgag ggcgacgtac agcatgccga
      721 gcaccgtgtc gttgtgcgcc atcggcgcgc ccaccgcgat gcccatgcga tcgcgcaggg
      781 cgcgcgcgcg ctcggcctgc gccaccgcac cggccatgcg gccgacgtag ccgagcgcgc
      841 cggccaggtt ggtgaggttg acgatggtct cggcgacgtc gcccagctcc tcggcgagcg
      901 tcaccgcgcg cgagatcagg cgaatcgcct ccggccagcg cgcggcctgg ctcagggcgt
      961 ggccatagtc ggtggcgaac ttgtagcgca gaagcagatc gcggctcttc tcgaagcgcg
     1021 actccacctt gtgcagcatc tccacggcct gcgacgcgcg gccgctgttg gccagggcca
     1081 gcgcctcgag gcgcacggcg tcgagctcgg ggcgtgactg ccgcaggcgg cgcgcagcgg
     1141 tgcgcgcgcc accggcgacc tcggcggcat cgacgaagcg actggccatc agcagcgtgc
     1201 tggcgcgagc cagctgcgcc tgcagccgct gctcgtcggt cgtcgcatcg accatgagcc
     1261 ggtcggtgag ggcgagcgcg gcgtcgatgg tgcgcacgat gagcatcggc tcgacggcgc
     1321 gcgcgcgcag ggcgaaggcg cgcgcggcgt cgccgccgcg accggcgcag tcgatggcct
     1381 gctcgatcag gctcagctcg tcttcgcgcc gcgagtggcg cagcgcgacg tcggcggcgg
     1441 tctcgaaggc cgtggccgcc gcggcccatt cctgcgcctc gcgccagtgc gaggcgatgc
     1501 gcgcggcggg cttgccggcc gactgcagga actcggcgac ggcgcggtgc aacgcctgtg
     1561 caatggcctc gggcaccgag cggagcacga cttcgtagag caggtcgtgg gcgaagccgc
     1621 agtcgcggat cacgtgcccg gattccagct cacgccaggc gtcggcgagc tcgaccaccg
     1681 ggcggccgac aacgtgagcg gccagctcga tgctgaagtc gagacctgca atcgcgatca
     1741 cccgcgcgag ggcgagcgcc ccggggctca gctgctcgag gcggcgagcg atcagcgcgc
     1801 cgacgtcgtg cggcgtgggc aggtcttcgg gcagcgcttc gaggagctgc gtgccgccgg
     1861 cgatcagtgc gcgcaaggtt tccagcacga acagcgggtt gccgccggtg tggcaccaca
     1921 gcggctgcgc ccagcgccgt gcgtcgagcc cgggaatctc cagcgcttcg agcagcgttt
     1981 cgaccgcggc cggatcgagc ggcccgagcc gaatctcggc gagcgcgtca cgatcgccgg
     2041 cgtgccgcca ttcgtcgagc agagtcggca cgtcgcccgc gcggcagccg atgcaccagg
     2101 cgatcggcgc gcccgtgcca ccgcacagcg ccggcagcgc ggcgagcgtg gcctcgtcgg
     2161 cgaattgcgc gtcgtcgagg acgacgagcg acaagcccgc tcgcgcccac gccgacagcg
     2221 actcggcgac ggcgcgctgg aacgcaccgg cgtcgagccg gccgggattc gccgtgccca
     2281 gctcgggcag cacgcgcgcg agctcgccgc gcagccagcc cggctgcggc tcgccgaagc
     2341 gcgccgccag ggcgcgcagc gcccgcgcca tcacggcgta gagcaggcga gcgtcgccag
     2401 gccgcgcggg cacgacgatc gcgccgtcga cgcgggcggc gtggtcttcg agcaggcgcg
     2461 tcttgccgat gccgggctcg ccgccgacca gggtggagcg caccgaggcg agtgcggcgg
     2521 cgagctgcgc gcgctccctt tcgcgcccca ccagccgggg cgggcgcagg atggcgatcg
     2581 gcatgggcgc cgccggcagc gggggta
//
LOCUS       DASRDG010000315         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1200198, whole genome shotgun sequence.
ACCESSION   DASRDG010000315 DASRDG010000000
VERSION     DASRDG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200198"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFA35_05215"
     CDS             complement(<1..710)
                     /locus_tag="VFA35_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZT55605.1"
                     /translation="MHDIIQQLEAKRARARLGGGEKRIAAQHGKGKLTARERIELLLD
                     EDTFEEWDMFVEHRNHDFGMQDDRPPGDGVVTGYGMINGRLVFVFSQDFTVFGGSLSE
                     AHAEKICKVMDQAMKVGAPVIGLNDSGGARIQEGVASLGGYAEVFQRNVMASGVVPQI
                     SMIMGPCAGGAVYSPAMTDFIFMVKDTSYMFVTGPEVVKTVTHEDVTAEDLGGGITHT
                     TKSGVADLAFENDVDALQ"
     gene            complement(728..1732)
                     /gene="meaB"
                     /locus_tag="VFA35_05220"
     CDS             complement(728..1732)
                     /gene="meaB"
                     /locus_tag="VFA35_05220"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZT55606.1"
                     /translation="MAVDPVDQALVSGVRSGDRRALAKSITLLESSRADHRARADALL
                     NALLPFTGQAMRLGISGVPGVGKSTFIEALGLALIAKGHRVAVLAIDPSSSISGGSIL
                     GDKTRMERLSVSPAAYIRPSPASGTLGGVAEKTREAMLVCEAAGYDVVIVETVGVGQS
                     ETAVAGMTDMFVLLQLPNAGDDLQAIKKGVMELADLVVINKADLDPDAATRAQACIDS
                     ALRTFGGRAASGHGTTRVLQLSALNGEGIDTFWAAVEAFHAKRRASGEFDARRKTQAL
                     AWMWDIVHARLHADFRRHPAVRGALPTTIRDVAEARVAPSSAARSLLNLFEQEPRQAT
                     "
     gene            complement(1739..>2751)
                     /locus_tag="VFA35_05225"
     CDS             complement(1739..>2751)
                     /locus_tag="VFA35_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZT55607.1"
                     /translation="LIIQEETHITSVVDPWAGSYMMESLTQEMADKAWAIIEEVDAMG
                     GMVKCVDSGWAKLKIEASAAEKQARIDSGKDVIVGVNKYKLATEDAIETLDIDNHMVR
                     DNQGARLKRLRANRDAAKTQAALDALTRCAETGEGNLLDLSIKAVRLRATVGEVSDAL
                     EKVWGRHRADTQKVTGVYAAAYDSAEGWDKLKAEIAQFAEDQGRRPRVMIAKLGQDGH
                     DRGAKVVATAFADLGFDVDMGPLFQTAEECARQAIENDVHAVGVSTLAAGHKTLVPAI
                     IESLKAQGADDIIVFVGGVIPRQDYEMLFDAGVKGIYGPGTPIPASAKDVLEQIRANV
                     KA"
BASE COUNT          380 a          975 c          897 g          499 t
ORIGIN      
        1 atctgcagcg catccacatc gttctcgaat gccagatcgg ccacgccgct cttggtggtg
       61 tgcgtgatgc cgccgcccag gtcttcggcg gtcacgtctt catgcgtcac ggtcttcacg
      121 acttcggggc cggtgacgaa catgtacgag gtgtccttca ccatgaagat gaagtccgtc
      181 atggccggcg aatacaccgc gccgcccgcg caggggccca tgatcatgct gatctgcggc
      241 accacgcccg aggccatcac gttgcgctgg aagacctcgg cgtagccgcc gagcgaggcc
      301 acgccctcct ggatgcgcgc gccgcccgaa tcgttcagcc cgatcaccgg cgccccgacc
      361 ttcatcgcct gatccatcac cttgcagatc ttctcggcgt gggcctcact gagcgagccg
      421 ccgaagaccg tgaagtcctg gctgaacacg aacaccagcc ggccattgat catgccgtag
      481 ccggtgacga cgccgtcgcc gggcggccgg tcgtcctgca tgccgaagtc gtggttgcgg
      541 tgctcgacga acatgtccca ttcctcgaac gtgtcttcgt cgagcagcag ctcgatgcgc
      601 tcgcgcgcgg tcagcttgcc cttgccgtgc tgggcggcga tgcgtttctc gccgccgccc
      661 aggcgggcgc gagcgcgctt ggcttcgagc tgctggatga tgtcgtgcat ggtggtcctt
      721 ctctcgctta tgtcgcttgt ctcggctctt gttcgaacag gttcagcagg ctgcgcgccg
      781 ccgacgacgg cgcgacgcgc gcctcggcca cgtcgcgaat cgtcgtcggc agtgcaccgc
      841 gcaccgcggg gtggcggcgg aagtcggcgt gcaggcgcgc atgcacgatg tcccacatcc
      901 aggccagcgc ctgcgtcttg cggcgcgcgt cgaattcgcc gctggcgcgg cgcttcgcat
      961 ggaacgcttc gaccgccgcc cagaaagtat cgatcccctc gccgttcagc gcgctgagct
     1021 gcagcactcg cgttgtccca tggccggacg ccgcgcggcc gccgaacgtg cgcagcgcgc
     1081 tgtcgatgca ggcctgcgcg cgcgtcgcgg catcggggtc gaggtcggcc ttgttgatga
     1141 cgacgaggtc ggcgagctcc atcacgccct tcttgatcgc ctgcaggtcg tcgccggcgt
     1201 tcggcagctg cagcagcacg aacatatcgg tcatgcccgc gaccgcggtc tcgctctggc
     1261 cgacgccgac cgtttcgacg atcaccacgt cgtagcccgc cgcttcgcag accagcatcg
     1321 cctcgcgcgt cttctcggcg acgccgccga gcgtgcccga cgccgggctc gggcggatgt
     1381 aggccgccgg gctgaccgaa agccgctcca tgcgcgtctt gtcgccgagg atcgagccgc
     1441 cggagatgct cgacgacggg tcgatcgcga gcaccgcaac ccgatggccc ttggcgatca
     1501 acgcgaggcc gagcgcctcg atgaaggtcg acttgcccac gccgggcaca cccgagatgc
     1561 cgagccgcat tgcctggccg gtgaacggga gcagcgcgtt gagcagcgcg tcggcacgcg
     1621 cgcggtggtc ggcacggctg gattcgagca gcgtgatcga cttcgccagg gcccggcggt
     1681 cgccgctacg cacgccgctc accaaggcct gatcgacggg atcgactgcc acggggcttc
     1741 aggctttgac gttcgcgcgg atctgctcga gcacgtcctt cgcgctcgcc gggatcggcg
     1801 ttcccggccc gtagatgcct ttcacgccgg catcaaacag catctcgtag tcctggcgcg
     1861 ggatcacgcc gccgacgaac acgatgatgt cgtcggcgcc ctgcgccttc agcgactcga
     1921 tgatcgccgg caccagcgtc ttgtggccgg cggcgagcgt gctgacgccg accgcgtgca
     1981 cgtcgttctc gatcgcctgg cgcgcgcact cctcggcggt ctggaacaac ggccccatgt
     2041 cgacgtcgaa gccgaggtcg gcgaacgcgg tcgcgacgac cttggcgccg cggtcgtggc
     2101 cgtcctggcc gagcttggcg atcatcacgc gcgggcgccg gccctggtct tcggcgaact
     2161 gggcgatctc ggccttcagc ttgtcccaac cttcggcgct gtcataagcg gctgcgtaca
     2221 caccggtcac cttttgcgta tcggcgcgat ggcgccccca gaccttctcg agcgcgtccg
     2281 aaacttcgcc gaccgtcgcg cgcaggcgta ccgccttgat cgacaggtcg agcaggttgc
     2341 cctcgccggt ctcggcgcat cgtgtcagcg cgtcgagcgc ggcctgggtc ttggcggcat
     2401 cgcggttcgc gcgcagccgc ttcagccgcg cgccctggtt gtcgcggacc atgtggttgt
     2461 cgatgtccag cgtctcgatc gcgtcctcgg tcgcgagctt gtacttgttg acgccgacga
     2521 tcacgtcctt gccggagtcg atgcgggcct gcttctcggc cgcgctggcc tcgatcttca
     2581 gcttggccca gcccgaatcg acgcacttga ccatgccgcc catcgcgtcg acctcctcga
     2641 tgatcgccca ggccttgtcg gccatctcct gcgtcagcga ctccatcatg tagctgccgg
     2701 cccacggatc gacgacgctg gtgatgtggg tctcttcctg gatgatcagc t
//
LOCUS       DASRDG010000316         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1653869, whole genome shotgun sequence.
ACCESSION   DASRDG010000316 DASRDG010000000
VERSION     DASRDG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653869"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFA35_05230"
     CDS             <1..573
                     /locus_tag="VFA35_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HZT55608.1"
                     /translation="TILLPLAYWGHLINGNKDFFDDPIWDRWIPKNQIVPSPGSQEFL
                     RFCKENGVEVFYVTSRDQDEHTFDYALEHLKVLGAPYADKDHLTVLRDTSNKQPRQDE
                     IAKRFDVVVYLGDSLNDFRRKYYVKNVDERMKLMEADRELFGRKYVLFPNPTDGHWMA
                     AIFGNSEPPPSDENRRLFKKAATRSAWDGR"
     gene            complement(639..1832)
                     /locus_tag="VFA35_05235"
     CDS             complement(639..1832)
                     /locus_tag="VFA35_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZT55609.1"
                     /translation="MTQEPLVQGVEIADIDTAQMRRASRELLSLALMNARNHSLRWAA
                     AFEASAAGAAPLRWQLGRLGWFQEYWIARNIERQRGECCDATRPKLASILPQADAMFE
                     SAATAPLIDLQTIRQYLVDTLETTLELLDGAAEDDDALYFYRLALLREDACVERFAVL
                     AQTLGFDTGLRAPPVTRPMRAPLLFPATRLQLGSASGGFVPDREKWAHEVALPEFQID
                     SQAITWAQYGEFVEDGGYDERQHWSEPGWAWVQAQGRRTPRFVDQLRQGVLQRRFGQL
                     LRVPLAQPAMHVSWFEADAWCRWAGRRLPGEAEWEAAAVQGATRGFRWGEVREWTAGT
                     LRPYPGAPATSSPGQSEPAGIGPRRVLRGASFATRGRLRHPKARAAEEPGFDEGFCGF
                     RSCAV"
     gene            1902..>2448
                     /locus_tag="VFA35_05240"
     CDS             1902..>2448
                     /locus_tag="VFA35_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZT55610.1"
                     /translation="MPRLIAHLDMDAFYASVELLRYPDLRGRAVVIGGGRRHQPVLLA
                     DGTRRYALLRDYTGRGVITTSTYEARALGVFSAMGTMKAAKLAPDAVLLPVDFDQYRH
                     YSRLFKAAVRAVAPVVEDRGIDEIYIDLTDLPGAQLDAGRAVAQRLKDAVLAATGGLT
                     CSIGVTPNKLLSKLASELDKPN"
BASE COUNT          388 a          909 c          814 g          337 t
ORIGIN      
        1 acgatcctgc tgccgctcgc gtactggggc cacctgatca acggcaacaa ggacttcttc
       61 gacgatccga tctgggatcg ctggatcccg aagaaccaga tcgttccgtc gcccggctcg
      121 caggagttcc tgcgcttctg caaggagaac ggcgtcgagg tcttctacgt gaccagccgc
      181 gaccaggacg agcacacgtt cgactacgcg ctcgaacacc tgaaggtcct gggtgcgccc
      241 tacgccgaca aggaccacct gacggtgctg cgcgacacca gcaacaagca gccgcgccag
      301 gacgagatcg ccaagcgatt cgacgtggtc gtgtacctgg gcgacagcct caacgacttc
      361 cgtcgcaagt actacgtcaa gaacgtggac gagcgcatga agctgatgga ggccgatcgc
      421 gagttgttcg ggcgcaagta cgtgctgttc ccgaacccga ccgatggcca ctggatggcg
      481 gcgatcttcg gcaactccga gccgccgccg agcgacgaga accgccggct gttcaagaag
      541 gcagcgaccc gatcagcctg ggacggtcga taaccatccc ccggcaaagc ccgggggcat
      601 tcgggatgtg agccgctcgc ctgtctccgc ctcgcggctc agaccgcgca gctgcgaaag
      661 ccgcagaaac cctcgtcgaa gccgggctcc tcggcggcgc gcgccttcgg gtggcgcagc
      721 cggccgcggg tcgcgaacga ggcgccgcgc agcacccgcc gcgggccgat gccggcgggc
      781 tcgctctggc cgggcgatga cgtcgccggc gcgcccggat acggccgcag cgtgccggcc
      841 gtccactcgc gtacctcgcc ccagcggaag ccgcgcgtcg cgccctgcac cgccgccgcc
      901 tcccactcgg cctcgccggg caggcgccgg ccggcccagc ggcaccaggc atcggcctcg
      961 aaccagctca catgcatcgc cggctgggcg agcggcacgc gcagcaactg gccgaagcgc
     1021 cgctgcagca cgccctggcg cagctgatcg acgaagcgcg gcgtgcgccg gccctgcgcc
     1081 tgcacccagg cccagccggg ctcgctccag tgctggcgct cgtcgtagcc gccgtcctcg
     1141 acgaactcgc cgtattgcgc ccaggtgatc gcctgcgagt cgatctggaa ttcgggcaat
     1201 gcgacctcgt gcgcccactt ctcgcggtcg ggcacgaaac cgcccgacgc gctgccgagc
     1261 tgcagccgcg tggcggggaa cagcagcggc gcgcgcatcg gccgcgtgac gggcggcgcg
     1321 cgcaagccgg tgtcgaaacc gagcgtctgc gcgagcaccg cgaagcgctc gacgcacgcg
     1381 tcctcgcgca gcagcgccag gcggtagaag tacagcgcgt cgtcgtcctc ggccgcgcca
     1441 tcgagcagct cgagcgtcgt ctcgagggtg tcgacgaggt attggcggat cgtctgcaga
     1501 tcgatcagcg gcgcggtcgc ggccgactcg aacatcgcat ctgcctgcgg caggatcgac
     1561 gcgagcttcg gccgcgtcgc gtcgcagcac tcgccgcgct ggcgctcgat gttgcgggcg
     1621 atccagtatt cctggaacca gcccagccgg ccgagttgcc accgcagcgg cgcagcgccg
     1681 gccgccgacg cctcgaacgc cgcggcccag cgcaggctgt ggttgcgcgc gttcatcagc
     1741 gccagcgaga gcaactcccg gctggcgcgg cgcatctgcg ccgtgtcgat atccgcgatc
     1801 tccacaccct gcaccagcgg ctcctgcgtc aagcgttgcg gacgattgtg gcagtgcgct
     1861 gatactgtac ataatcccag cctcgcccca caatccaccg catgccccgc ctgatcgccc
     1921 acctcgacat ggacgcgttc tacgcctcgg tcgagctgct gcgctacccg gacctgcgcg
     1981 gccgcgcggt cgtgatcggc ggcgggcgcc ggcaccagcc ggtcctgctg gccgacggca
     2041 cgcggcgcta cgcgctgcta cgcgactaca ccggccgcgg cgtgatcacc accagcacct
     2101 acgaggcgcg cgcgctcggc gtgttctcgg cgatgggcac catgaaggcg gccaagctcg
     2161 cgcccgacgc ggtgctgctg ccggtcgact tcgaccagta ccggcactac tcgcggctgt
     2221 tcaaggccgc ggtgcgcgcg gtcgcgcccg tcgtcgaaga ccgcggcatc gacgagatct
     2281 acatcgacct gaccgacctt cccggcgcgc aactcgacgc cggccgcgcg gtcgcgcagc
     2341 ggctgaagga cgcggtcttg gccgccaccg gcggcctcac ctgctcgatc ggcgtgacgc
     2401 cgaacaagct gctgtccaag ctcgcctccg agctcgacaa gccgaacg
//
LOCUS       DASRDG010000317         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_279414, whole genome shotgun sequence.
ACCESSION   DASRDG010000317 DASRDG010000000
VERSION     DASRDG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279414"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFA35_05245"
     CDS             <1..1029
                     /locus_tag="VFA35_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZT55611.1"
                     /translation="DSIVKGLTMDQAVKRMRGEPNTKVVLTIFRKTENRSFPVTIMRE
                     EIRVQSVRAKVIEPGYAWLRVSQFQDRTVDDFARKLEEIYKAEPNLKGLVLDLRNDPG
                     GLLEGAVAISAAFLPADVVVVSTNGQIADSKQTFKASPSDYLRRGGSDPLRRLPEALK
                     KVPLIVLVNEGSASASEIVAGALQDHKRATLMGAQTFGKGSVQTVRQLSPETALKITT
                     ARYYTPSGASIQAKGIVPDVMLDETAEGNLFAALRTREADLEKHLQSGQGPEVKDEAR
                     DKAREEARKKLEEEAAKRAAAPAKPPVEFGSTEDFQLARALNRLKGKPVLVSKTAVER
                     KPETDTVN"
     gene            1094..1846
                     /locus_tag="VFA35_05250"
     CDS             1094..1846
                     /locus_tag="VFA35_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HZT55612.1"
                     /translation="MTDDQLLRYSRHILLDELGIEGQQRLLGAHALVIGAGGLGSPVA
                     LYLATAGVGTLTIVDGDTVDLTNLQRQIVHRVDRIGEPKAESAARTLQAINPEVNVIA
                     LRERADAQRLRELVADADVVVDCSDNFATRHGVNAACVAWAKPLVSGAAIGFDGQISV
                     YDTRDATKPCYACLFPPDAVFEEVRCATMGVFAPLVGIIGSVQAAEALKLLAGVGSSL
                     AGRLQMLDARTMEWTEIAMARTPGCPVCGHRG"
     gene            1935..2705
                     /locus_tag="VFA35_05255"
     CDS             1935..2705
                     /locus_tag="VFA35_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZT55613.1"
                     /translation="MSAPLFEAIAPEAAGMPLYQVVKRSLLNAIESGGCAPGETLPSE
                     TEIAGAMGVSIGTLRRAVDELAAEHILVRRQGRGTFVATHGTDRFLFQFFHVERADGL
                     REAPVVDLVSFERARADGEPAAALHLRVGDPVIQIENRLHLQGAAVIYDRLTLPSTLF
                     KGLTEKRFRERPGTIYQLYQSDFGISVLRANERARATSADRSAVRVLSLALGQPVMQV
                     RRTALTFGDRPVEHRVSIIDTAHHDYVNVLSRPQGVNS"
BASE COUNT          469 a          965 c          941 g          389 t
ORIGIN      
        1 gacagcatcg tcaagggcct gacgatggac caggccgtca agcgcatgcg cggcgagccc
       61 aacacgaagg tcgtgctcac gatcttccgc aagaccgaga accgcagctt ccccgtcacg
      121 atcatgcgcg aggagatccg cgtgcagagc gtgcgcgcca aggtcatcga gcccggctat
      181 gcctggctgc gcgtgagcca gttccaggac cgcaccgtcg acgacttcgc ccgcaagctc
      241 gaagagatct acaaggccga gccgaacctg aagggcctgg tgctcgacct gcgcaacgac
      301 ccgggcggcc tgctcgaagg cgcggtcgcg atctcagccg cgttcctgcc cgccgacgtg
      361 gtggtggtct cgaccaacgg ccagatcgcc gactcgaagc agaccttcaa ggcctcgccg
      421 agcgactacc tgcgccgcgg cggcagcgac ccgctgcgcc gtctgcccga ggcgctgaag
      481 aaggtgccgc tgatcgtgct ggtcaacgaa ggctcggcct cggccagcga gatcgtcgcc
      541 ggagcgctgc aagaccacaa gcgcgccacg ctgatgggcg cgcagacctt cggcaagggc
      601 tcggtgcaga ccgtgcgcca gctctcgccc gagaccgcgc tgaagatcac gaccgcgcgc
      661 tactacacgc cgagcggcgc gtcgatccag gccaagggca tcgtgcccga cgtgatgctc
      721 gacgaaaccg ccgagggcaa cctgtttgcc gcgctgcgca cccgtgaggc cgacctcgaa
      781 aagcacctcc aaagcggcca gggccccgag gtgaaggacg aagcgcgcga caaggcgcgc
      841 gaagaggccc gcaagaagct cgaggaagag gccgcgaagc gcgccgccgc gccggccaag
      901 ccgccggtgg agttcggcag caccgaggac ttccagctcg cgcgggcgct gaaccgcctg
      961 aagggcaagc cggtgctggt cagcaagacc gccgtcgaac gcaagcccga gaccgacacg
     1021 gtgaactaaa ggccccgcgt ttcacccgag cgcccgcgcg aaccgcgggc gcttttgttt
     1081 gtatgctcga atcatgactg acgaccagtt gctgcgctac tcccgccaca tcctgctcga
     1141 cgagctcggc atcgaaggcc agcagcggct gctgggcgcg cacgcgctgg tgatcggggc
     1201 cggcgggctg ggctcgccgg tggcgttgta cctcgcgacg gccggggtcg gcacgctgac
     1261 gatcgtcgac ggcgataccg tcgacctgac gaacctgcag cggcagatcg tgcaccgcgt
     1321 cgatcgcatc ggcgagccga aggccgagtc cgccgcgcgg acgctgcagg caatcaaccc
     1381 cgaggtgaac gtgatcgcgc tgcgcgagcg cgccgatgcg cagcgcctgc gcgaactggt
     1441 cgccgacgcc gacgtggtcg tcgactgcag cgacaacttc gcgacccgcc acggcgtcaa
     1501 cgcggcgtgc gtcgcgtggg ccaagccgct cgtctcgggc gcggcgatcg gcttcgacgg
     1561 gcagatctcg gtctacgaca cgcgcgacgc gacgaagccc tgctacgcct gtctgttccc
     1621 gcccgacgcg gtgttcgagg aagtgcgctg cgcgacgatg ggcgtgttcg cgccgctggt
     1681 gggcatcatc ggcagcgtgc aggcggccga ggcgctgaag ctgctcgccg gcgtgggcag
     1741 ctcgctcgcc gggcggctgc agatgctcga tgcgcgaacg atggaatgga ccgagatcgc
     1801 gatggcgcgc acgcccggct gcccggtatg cggccaccgg ggctgaattc gaccgaatcg
     1861 tttcaaatag atcatataga tgacttactt cacacaaatg atctgcatgt atacttcggg
     1921 tcatgcagac tgccgtgtcc gccccgctgt tcgaggccat cgcgcccgag gcggcgggca
     1981 tgccgctgta ccaggtcgtc aagcgctcgc tgctgaacgc gatcgagtcg ggcggttgcg
     2041 cgccgggtga gacgctgccg agcgagaccg agatcgccgg cgcgatgggc gtctcgatcg
     2101 gcacgctgcg ccgcgccgtc gacgagctcg ctgccgagca catcctggtg cggcgccagg
     2161 gacgcggcac tttcgtcgcg acccacggca ccgaccgctt cctgttccag ttcttccacg
     2221 tcgagcgcgc cgacggactg cgcgaggccc cggtcgtcga cctggtctcg ttcgagcggg
     2281 cgcgcgccga cggcgaaccc gccgcagcgc tgcatctgcg cgtcggcgac cccgtgatcc
     2341 agatcgagaa ccggctgcac ttgcagggcg cagcggtgat ctacgaccgg ctcaccctgc
     2401 cgtcgaccct gttcaagggc ctgaccgaga agcgcttccg cgagcgcccg ggcacgatct
     2461 accagctcta ccagtcggac ttcggcatca gcgtgttgcg cgcgaacgag cgcgcccgcg
     2521 cgacctcggc cgaccgcagc gcggtgcgcg tgctgagcct ggcgctcggc caacccgtga
     2581 tgcaggtgcg ccgcaccgcg ctgaccttcg gcgaccgccc tgtcgaacat cgggtttcga
     2641 tcatcgacac ggcacatcac gactacgtga acgtgctgtc tcgaccccag ggggtgaact
     2701 catgaccggc tcgacgaccg atctcctgaa ggcggctcgg gccgcggagc aggcggccta
     2761 cgcc
//
LOCUS       DASRDG010000318         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_408752, whole genome shotgun sequence.
ACCESSION   DASRDG010000318 DASRDG010000000
VERSION     DASRDG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408752"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFA35_05260"
     CDS             <1..183
                     /locus_tag="VFA35_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZT55614.1"
                     /translation="EIAQAFTTERARDFHVVPAVEALAKAHVAQADTLVRGDYLRLWP
                     HRHGTDGFFAAVWERR"
     gene            309..1532
                     /locus_tag="VFA35_05265"
     CDS             309..1532
                     /locus_tag="VFA35_05265"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZT55615.1"
                     /translation="MDSLTLVWDALINWLANGLLGASWWQVLLFALVATHVTIASVTI
                     FLHRAQAHRALELHAIPSHFFRLWLWLTTGMVTKEWVAIHRKHHAKCETEDDPHSPQT
                     RGIKAVLLTGSEMYRAEAKVQDTLDKFGHNTPNDWIERNLYSRYSWQGVGVMLLIDLA
                     LFGAIGAAVWALQMAWIPVTAAGIINGLGHWWGYRNFEAPDASTNISPWGILIGGEEL
                     HNNHHTYPTSAKLSVKPYEFDIGWAYIRALEMAGLAQVRKTVPLLKLGTVKPVADDKT
                     LEAIIANRYEVMAKYARNLRHASRAEVARLKAEGAASAAKLENMRLANRWLHRDADKI
                     PMDVKARVAAAVEHSPALAKLVAMREELRLLWTRTNVSAEQLVADLQAWCKNAEESGI
                     AALQEFALKLRAAKA"
     gene            complement(1665..1832)
                     /gene="rpmG"
                     /locus_tag="VFA35_05270"
     CDS             complement(1665..1832)
                     /gene="rpmG"
                     /locus_tag="VFA35_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZT55616.1"
                     /translation="MAKGGREKIKLESSAGTGHFYTTDKNKKTTPEKLEMVKFDPKAR
                     KHVTYKETKLK"
     gene            complement(1848..2081)
                     /gene="rpmB"
                     /locus_tag="VFA35_05275"
     CDS             complement(1848..2081)
                     /gene="rpmB"
                     /locus_tag="VFA35_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZT55617.1"
                     /translation="MARVCQVTGKGPMVGNNVSHANNKTKRRFLPNLQYRRFWVEAEN
                     RWVRLRISNAALRLIDKVGIEKVLVDLRARGEI"
     gene            complement(2178..>2823)
                     /locus_tag="VFA35_05280"
     CDS             complement(2178..>2823)
                     /locus_tag="VFA35_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT55618.1"
                     /translation="TYTCDALIIATGASAKYIGLPSEEAFMGRGVSGCATCDGFFYRE
                     QDVCVVGGGNTAVEEALYLSNIASKVHLIHRRDKFRAEPILVDKLHARVAEGKMTLHL
                     WQALDEVLGDDSGVTGVRLKSTENGATSELKLTGCFIAIGHQPNTDIFKGQLEMKDGY
                     LITKSGLSGMATMTSVSGVFAAGDVQDHVYRQAITSAGTGCMAALDAQRFLEQQ"
BASE COUNT          490 a          908 c          911 g          514 t
ORIGIN      
        1 gagatcgcgc aggccttcac gaccgagcgc gcacgcgatt ttcatgtcgt gccggccgtc
       61 gaggcactgg ccaaggcgca cgtcgcgcag gccgacaccc tggtgcgcgg cgactacctg
      121 cgactgtggc cgcaccgcca cggtaccgac ggttttttcg ccgcggtgtg ggagcggcgt
      181 tgagattgcg ccgttcgcct tgaagtggcc ctcaaccggc aaggtcatat ccgccggagc
      241 gaaggggttt acccggccct gtcccttaca atggcgtacc tgcgtttcaa gcaggttcaa
      301 aggtaagaat ggattcgttg acattggttt gggatgcgct gatcaactgg ctggccaatg
      361 gcctgctcgg tgcatcgtgg tggcaggtgc tgttgttcgc gctggtcgcg acacacgtga
      421 cgatcgcgtc ggtgacgatc ttcctgcacc gcgcgcaggc gcaccgcgcg ctcgagttgc
      481 acgcgatccc gtcgcatttc ttccggctct ggctgtggct gaccaccggc atggtgacca
      541 aggagtgggt cgcgatccac cgcaagcacc acgccaagtg cgagaccgaa gacgatcccc
      601 acagcccgca gacccgcggc atcaaggccg tgctgttgac cggcagcgag atgtaccgcg
      661 ccgaagccaa ggtgcaggac acgctcgaca agttcggcca caacacgccg aacgactgga
      721 tcgagcgcaa cctctacagc cgctacagct ggcagggcgt gggcgtgatg ctgctgatcg
      781 acctggcgtt gttcggcgcg atcggcgcgg ccgtgtgggc gctgcaaatg gcgtggatcc
      841 cggtcaccgc cgccggcatc atcaatggcc tcggccactg gtggggctac cgcaatttcg
      901 aggcgcccga tgcgtcgacc aacatctcgc cgtggggcat cctcatcggc ggcgaagagt
      961 tgcacaacaa ccaccacacc tatccgactt cggccaagct gtcggtcaag ccctacgagt
     1021 tcgacatcgg ctgggcctac atccgcgcgc tcgagatggc gggtctggcc caggtgcgca
     1081 agaccgtgcc gctgctgaag ctcggcaccg tcaagccggt cgccgacgac aagacgctcg
     1141 aggcgatcat cgcgaaccgc tacgaggtga tggccaagta cgcgcgcaac ctgcgccacg
     1201 ccagccgcgc cgaagtggcg cgcctgaagg ccgagggcgc agcgagcgcc gcgaagctcg
     1261 agaacatgcg cctggcgaac cgctggctgc accgcgacgc ggacaagatc ccgatggacg
     1321 tcaaggcacg cgtggccgcg gccgtcgagc acagcccggc gctggccaag ctggtcgcga
     1381 tgcgcgagga actgcgcctg ctctggaccc gcaccaacgt gtcggccgag caactggttg
     1441 ccgatctgca agcctggtgc aagaacgcgg aagagagcgg catcgccgct ctgcaggagt
     1501 tcgccttgaa gctgcgcgct gccaaggcgt aggacacggc gcctaccgag ccgaccaaac
     1561 gaaagggccg cgaatgcggc cctttcgttt ggagacagcc aaagagaaag aaccaaagag
     1621 aagggcccgc aacgcgggcc cgccatcgtc tccagccgga ggcgctactt caatttcgtt
     1681 tctttgtagg tcacgtgctt gcgtgccttc gggtcgaact tgaccatctc cagcttttcg
     1741 ggcgtggtct tcttgttctt gtcggtcgtg tagaaatgac cggtcccggc gctggattcc
     1801 agcttgatct tctcgcgtcc accttttgcc atgatcagct cccggggtta gatttcgccg
     1861 cgggcgcgga ggtcgacgag gaccttctcg atgcccacct tgtcgatcag gcgcagcgcc
     1921 gcgttcgaga tgcgcagacg cacccagcgg ttctcggcct cgacccagaa gcggcggtac
     1981 tgcaggttcg gcagaaagcg acgcttcgtt ttgttgttcg cgtgggaaac gttgttaccc
     2041 accatcgggc ccttgcccgt gacctgacag acgcgtgcca tgacgcttct ccaattcaat
     2101 caaatgatct agctgccgga atttcggcaa agactgcgat tatagaccga tttgcggggc
     2161 cgccaaccgg cggccggtca ctgttgctct aggaagcgct gcgcgtccag cgcggccatg
     2221 cagccggtgc cggcgctggt gatcgcctga cggtagacat gatcctgcac atcgccggcg
     2281 gcgaacacgc ccgacacgct ggtcatcgtc gccatgcccg agaggccgct tttcgtgatc
     2341 aggtagccgt ccttcatctc gagctggccc ttgaagatgt cggtgttcgg ttggtggccg
     2401 atcgcgatga agcagccggt cagtttcagc tcgctggtcg cgccgttctc ggtactcttc
     2461 aggcgcacgc cggtcacgcc ggagtcgtcg cccagcactt cgtcgagcgc ttgccacagg
     2521 tgcaacgtca tcttgccttc ggcgaccctg gcgtgcagct tgtcgaccag gatcggctcg
     2581 gcgcggaact tgtcgcgccg atggatcagg tgcaccttgc tggcgatgtt cgacaggtac
     2641 agcgcttctt cgacggcggt gttgcccccg ccgacgacgc agacgtcctg ctcgcggtag
     2701 aagaagccgt cgcaggtggc gcagccgctg acgccgcgcc ccatgaaggc ctcttcactc
     2761 ggcagcccga tgtacttggc gctggcgccg gtggcgatga tcagcgcgtc gcaggtgtag
     2821 gtg
//
LOCUS       DASRDG010000319         5812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_161869, whole genome shotgun sequence.
ACCESSION   DASRDG010000319 DASRDG010000000
VERSION     DASRDG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161869"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VFA35_05285"
     CDS             <1..923
                     /locus_tag="VFA35_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZT55619.1"
                     /translation="EDLGGGDVHTRLSGVADHLAQNDTHALAIARASVANLNWRKEHG
                     QRLVAPRPPLFAPEELHGVIPADTKKPYDVREIIARIVDGSEFDEFKTRYGATLVTGF
                     AHIEGMPVGIIANNGVLFSESAQKGAHFIELCCQRKIPLVFLQNITGFMVGKKYEAEG
                     IAKHGAKMVTAVSTATVPKFTVIIGGSFGAGNYGMCGRAYSPRLLWMWPNARISVMGG
                     EQAASVLATVKRDGIEAKGGAWSKDEEAAFKQPILDQFEHQAHPYYASARLWDDGVID
                     PAQTRTVLALAISASLNAPIPDAKFGVFRM"
     gene            964..1275
                     /locus_tag="VFA35_05290"
     CDS             964..1275
                     /locus_tag="VFA35_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HZT55620.1"
                     /translation="MRTNIDIDDALMSEAMKAGPFQTKKEAVEAGLKLLARQVAYREI
                     LKWRGKLKWEGDESIDWTRPETPVVSEALPAAWPKPKRAGREPAPAAEPAAPRKRRAG
                     R"
     gene            1262..1663
                     /locus_tag="VFA35_05295"
     CDS             1262..1663
                     /locus_tag="VFA35_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HZT55621.1"
                     /translation="MLVVDSSVWIDFFNDAGTPACDLLDHLLDNGEVRIVVPDLVLFE
                     VLRGFRSERDHRQAKRLMESLSVEGTAGHQLALAAAGHYRSLRAQGITVRGPIDVLVA
                     SFCIERDYALLHGDKDFGAFESLRGLRGWRH"
     gene            1665..2453
                     /locus_tag="VFA35_05300"
     CDS             1665..2453
                     /locus_tag="VFA35_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZT55622.1"
                     /translation="MKPTALQIEREGHVARVWLNRPEVRNAFNEGVIAEMHAAFTAFA
                     ADATLRAIVLGGRGKAFCAGADLAWMRAMAGYDWERNRADAQALADMLWAIYRCPVPV
                     VGRVHGDCYAGGVGLAAVCDVLVAADGANFCLSEAKLGLLPGTIAPYVIRALGEQAAR
                     RYFVTAERFGAAQAHARGFVHELVAAEALDAKVDEIVAALVANGPMAVRACKQLVQDV
                     AGAPLDAALRDETARRIADIRASDEGREGVQSFLNKRPPNWLEG"
     gene            2459..3082
                     /locus_tag="VFA35_05305"
     CDS             2459..3082
                     /locus_tag="VFA35_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HZT55623.1"
                     /translation="MNALPIDTTGLLAIAAALGWASGLRLYAVVFLTGLAGHLGWVQL
                     PAGLHVLQSNAMLAASGLMLFVEFFADKIPGLDSLWDMVHTVVRIPAGAALAAAVFGA
                     DQASWAMAAALMGGTLAATSHVAKATTRAALNTSPEPFSNVAMSLLGDSVVPASLWLS
                     WTHPYLFFAALAVALVVMLALTWVFAKFLRRLARRVSGWFGAGQPSV"
     gene            3090..5087
                     /locus_tag="VFA35_05310"
     CDS             3090..5087
                     /locus_tag="VFA35_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZT55624.1"
                     /translation="MFQKILIANRGEIACRVAATARRLGIRTVAVYSDADANAKHVLA
                     CDEAVHIGGSAPRDSYLRGDRVIAAALATGAQAVHPGYGFLSENAAFAQACADAGLVF
                     IGPPPSAIEAMGLKAESKRLMAQAGVPLVPGYHGAGQDAPMLKGEADRIGYPVLIKAS
                     AGGGGKGMRIVAKADEFDAALASCQREAINSFGSAAVLIERYVTRPRHIEIQVFADTH
                     GDCVYLFERDCSVQRRHQKVLEEAPAPGISEARRAEMGAAAVAAAQAVGYVGAGTVEF
                     IAEPGEDGDLRFFFMEMNTRLQVEHPVTEAITGLDLVEWQLRVAAGEPLPLRQDELRI
                     HGHAIEARICAENPDANFLPATGRLEVYRTPAGAVSFERGDVRVDAGVREGDAVLPFY
                     DSMIAKLIVWGADRAQALARLDAALAATHIVGLHTNVAFLRRVAASESFAHADLDTAL
                     IERERAALFNAAPLPIELAAAGVVAHTLAAEQALQTADPWSRRDGWRLHGRATRRFDI
                     AAQGTHHPFTLERGAGAQTLANAAQRWSLAIAPRAPNTCDVTLGEQRTTLTVYATGER
                     FSVFSPDGAAVVEEFDPIAHAADGAVETGGLAAPMPGKVIAFLAQAGDHVKQGQPLAV
                     MEAMKMEHTIAAPRDGTIAELLYAVGDQVGEGGELLRLAPA"
     gene            5114..>5812
                     /locus_tag="VFA35_05315"
     CDS             5114..>5812
                     /locus_tag="VFA35_05315"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZT55625.1"
                     /translation="MLPTHVTLVEVGPRDGLQNEELPVDAATKIELVHRLQDAGLREI
                     EVTSYVSPKWVPQMADNAQVMRGVRRKSGVRYSVLTPNLKGFEAAMADPAIRPDEIVV
                     FGAASEAFSQRNINCGIEESVERFRPVVEAAHAQKIKVRCAISVALGCPYQGEVTADA
                     VERVVVLMKAIGVDHCGIADTIGVGTPRRARAALERALKHFPLAEVSGHFHDTYGQAL
                     ANVYACLEMGIHTFD"
BASE COUNT          881 a         1984 c         2101 g          846 t
ORIGIN      
        1 ccgaggacct gggcggtggc gacgtgcaca cccggctgtc gggcgtggcc gaccacctgg
       61 cgcaaaacga cacccacgcg ctggcgatcg cacgcgcgtc ggtcgcgaac ctgaactggc
      121 gcaaggagca cggccagcgc ctggtcgcgc cgcgcccgcc actgttcgcg cccgaagagc
      181 tgcacggcgt gatcccggcc gacaccaaga agccgtacga cgtgcgcgag atcatcgcgc
      241 gcatagtcga cggctcggag ttcgacgagt tcaagacgcg ctacggtgcg acgctggtga
      301 ccggtttcgc gcacatcgag ggcatgccgg tgggcatcat cgcgaacaac ggcgtgctgt
      361 tcagcgagtc ggcgcagaag ggcgcgcact tcatcgagct gtgctgccag cgcaagatcc
      421 cgctggtgtt cctgcagaac atcaccggct tcatggtcgg caagaagtac gaggccgagg
      481 gcatcgccaa gcacggcgcg aagatggtca ccgcggtctc gaccgcgacc gtgcccaagt
      541 tcaccgtgat catcggcggc agcttcggcg ccggcaacta cggcatgtgc gggcgcgcgt
      601 actcgccgcg cctgctgtgg atgtggccga acgcgcgcat cagcgtgatg ggtggcgagc
      661 aggcggcgag cgtgctcgcg accgtcaagc gcgacggcat cgaggccaag ggcggcgcat
      721 ggtcgaagga cgaggaggcc gcgttcaagc agccgatcct cgaccagttc gagcaccagg
      781 cccacccgta ctacgccagc gcgcgcctgt gggacgacgg cgtgatcgac ccggcgcaga
      841 cccgcaccgt gctcgcgctg gcgatctcgg cgagcctgaa cgcgccgatc ccggacgcga
      901 agttcggggt cttccggatg tgatggtgta aactgtgtac atgttaggga gttcatacac
      961 atcatgcgca ccaacatcga tatcgacgac gccttgatga gcgaggcgat gaaggccggg
     1021 cctttccaaa ccaagaagga agcggtcgag gcggggttga agttgcttgc acggcaagtc
     1081 gcctaccgcg agattctcaa gtggcgcggc aagctcaagt gggaaggcga cgagtcgatc
     1141 gactggaccc ggcccgagac gcccgtcgtc tcggaggcct tgccggccgc gtggccgaag
     1201 ccgaagcgcg ccggtcgcga gcccgctccg gccgccgagc ccgctgcacc gaggaagcgc
     1261 cgtgctggtc gttgactcgt cggtctggat cgacttcttc aacgatgcgg gcacgcctgc
     1321 gtgcgacttg ctcgatcacc tgctggacaa cggcgaagtc cggatcgtcg tcccggatct
     1381 cgtgctgttc gaggtcttgc gcggcttccg ctccgaacgg gatcaccgcc aggccaagcg
     1441 gctgatggag tccctgtcgg tggaagggac ggccgggcat cagttggcgc ttgcggctgc
     1501 cgggcactat cgcagcctgc gcgcgcaagg catcacggtg cgcggcccga tcgacgtgct
     1561 ggtggcgagc ttttgcatcg agcgtgacta cgccttgctg cacggtgaca aggacttcgg
     1621 cgcattcgag tcgctgcgcg gcttgcgcgg ctggagacac tgaagtgaaa cccaccgcat
     1681 tgcagatcga gcgcgagggc catgtcgcgc gcgtctggct caaccgcccc gaggtgcgca
     1741 atgccttcaa cgagggcgtg atcgccgaga tgcacgccgc gttcaccgca ttcgcggccg
     1801 acgccacgct gcgcgcgatc gtgctcggcg gccgcggcaa ggccttctgc gccggcgccg
     1861 atctggcgtg gatgcgggcg atggccggct acgactggga acgcaaccgc gccgatgcgc
     1921 aggcgctggc cgacatgctg tgggcgatct accgctgccc ggtgccggtg gtcggccgcg
     1981 tgcacggcga ctgctatgcc ggcggcgtcg gcctcgcggc ggtctgcgac gtgctggtcg
     2041 cggccgacgg cgcgaacttc tgcctcagcg aagccaagct cgggctgctg cccggcacga
     2101 tcgccccgta cgtgatccgc gcgctcggcg agcaggcggc gcgacgctac ttcgtcaccg
     2161 ccgagcgctt cggcgcggcg caggcgcacg cgcggggctt cgtgcacgag ctcgtcgcgg
     2221 ccgaggcgct cgacgcgaag gtcgacgaga tcgtcgcggc gctggtcgcc aacggaccga
     2281 tggccgtgcg tgcctgcaag cagctcgtgc aggacgtggc cggcgcgccg ctcgacgccg
     2341 cgctgcgcga tgagaccgcg cgccgcatcg ccgatatccg cgcgagcgac gagggccgcg
     2401 agggcgtgca gagcttcctg aacaagcgcc cgccgaattg gttggagggg tgagcccgat
     2461 gaacgcgttg ccgatcgaca ccaccggcct gctcgcgatc gccgctgcgc tcggctgggc
     2521 gagcggactg cgcctctatg cggtcgtgtt cctgaccggt ttggccggtc acctgggctg
     2581 ggtgcagctg cccgcgggcc tgcacgtgct gcagtccaac gcgatgctcg cggcgagcgg
     2641 cctgatgctg ttcgtcgagt tcttcgccga caagatcccc gggctcgatt cgctgtggga
     2701 catggtccac acggtcgtgc gcattccggc cggcgcggcg ctcgcggcgg cggtgttcgg
     2761 cgccgaccag gccagctggg cgatggccgc ggcgctgatg ggcggcacgc tggccgcgac
     2821 cagccacgtc gccaaggcca cgacgcgcgc cgcgctcaac acctcaccgg agccgttttc
     2881 gaacgtcgcg atgtcgctgc tcggcgacag cgtcgtgccg gcgagcctgt ggctgtcgtg
     2941 gacgcatccg tacctctttt tcgcggcgct cgcggtggcg ctcgtcgtga tgctggcgct
     3001 gacctgggtg ttcgcgaaat tcctgcgccg ccttgcgcgg cgcgtgtcgg gctggttcgg
     3061 ggcgggccaa ccgtcagtgt gaggatcaca tgttccagaa gattctgatt gccaaccgcg
     3121 gcgagattgc ctgccgggtc gcggcgaccg cccgccggct cggcattcgc accgtcgccg
     3181 tctattccga cgccgacgcg aacgccaagc acgtgctggc ctgcgacgag gcggtgcaca
     3241 tcggcgggtc ggcgccgcgc gacagctacc tgcgcggcga ccgcgtgatc gccgcggcgc
     3301 tggcgaccgg cgcgcaggca gtgcaccccg ggtacggctt cctgagcgag aacgcggcct
     3361 tcgcgcaggc ctgcgccgat gccgggctgg tgttcatcgg cccgccgccg tcggcgatcg
     3421 aggcgatggg cttgaaggcc gagtcgaagc ggttgatggc gcaggccggc gtgccgctcg
     3481 tgccgggcta ccacggtgcc gggcaggatg cgccgatgct gaaaggcgag gccgaccgca
     3541 tcggctaccc ggtgctgatc aaggccagcg ccggcggcgg cggcaaaggc atgcgcatcg
     3601 tggcaaaggc cgacgagttc gacgccgcgc tcgcctcgtg ccagcgcgag gcgatcaaca
     3661 gcttcggcag cgcggcggtg ctgatcgagc gctacgtgac ccggccgcgc cacatcgaga
     3721 tccaggtgtt cgccgacacg cacggcgact gcgtctacct gttcgagcgc gactgctcgg
     3781 tgcagcgccg ccaccagaag gtgctcgagg aggcgccggc gccgggcatc agcgaagcgc
     3841 gccgcgccga gatgggcgcg gcggcggtcg cggcggcgca ggcggtcggc tatgtcggcg
     3901 cgggcacggt cgagttcatc gccgaacccg gggaagacgg cgacctgcgc ttcttcttca
     3961 tggagatgaa cacgcggctg caggtcgagc acccggtcac cgaggcgatc accgggctcg
     4021 atctggtcga gtggcaattg cgcgtcgccg cgggcgagcc gctgcctttg cgacaggacg
     4081 agttgcgcat ccacggccac gcgatcgagg cgcgcatctg cgccgagaac ccggacgcga
     4141 acttcctgcc cgccaccggc cggctcgagg tctaccgcac gccggcgggc gcggtcagct
     4201 tcgagcgggg tgacgtgcgg gtcgacgccg gcgtgcgcga gggcgatgcg gtgctgccgt
     4261 tctacgactc gatgatcgcc aagctgatcg tctggggtgc cgaccgcgcg caggcgctgg
     4321 cgcggctcga cgcggcgctg gcggcgacgc acatcgtcgg gctgcacacc aacgtcgcgt
     4381 tcctgcgccg cgtcgcggcg agcgaatcgt tcgcgcacgc cgatctcgac accgcgctga
     4441 tcgagcgcga gcgcgcggca ctgttcaacg cggcgccgct gccgatcgaa ctggcggcgg
     4501 ccggcgtcgt cgcgcacacg ctcgcggccg agcaggcgct gcagaccgcc gatccgtggt
     4561 cgcgccgcga cggctggcgc ctgcacggcc gcgcgacgcg ccgcttcgac atcgccgcgc
     4621 aaggcacgca ccacccgttc acgctcgaac gcggtgcggg cgcgcagacg ctcgcgaacg
     4681 ccgcgcagcg ctggtcgctc gcgatcgcgc cgcgcgcgcc caacacctgc gacgtgacgc
     4741 tcggcgagca gcgcacgacg ctgaccgtct acgccaccgg cgagcgcttc agcgtgttct
     4801 cgcccgacgg cgcggcggtc gtcgaggagt tcgacccgat cgcccatgcg gccgatggcg
     4861 cggtcgagac cgggggcctc gctgcgccga tgccgggcaa ggtgatcgcg ttcctcgccc
     4921 aggccggcga tcatgtcaag cagggccagc cgctcgcggt gatggaggcg atgaagatgg
     4981 aacacacgat cgcggcgccg cgcgacggca cgatcgccga gctgctctat gccgtgggcg
     5041 accaggtcgg cgagggcggc gagctgctgc gcctggcccc cgcgtgacaa tagccactgg
     5101 gagacacctt gccatgctgc caacccacgt gaccctggtc gaagtcggtc cgcgcgacgg
     5161 cctgcagaac gaggagctgc cggtcgacgc ggcgaccaag atcgagctgg tgcaccggct
     5221 ccaggatgcc ggcctgcgcg aaatcgaggt caccagctac gtcagcccga agtgggtgcc
     5281 gcagatggcc gacaacgcgc aggtgatgcg cggcgtgcgg cgcaagagcg gggtgcgcta
     5341 ctcggtgctg acgccgaacc tgaagggctt cgaggccgcg atggccgacc cggccatccg
     5401 ccccgacgag atcgtcgtgt tcggcgccgc cagcgaggcc ttcagccagc gcaacatcaa
     5461 ctgcggcatc gaagaaagcg tcgagcgctt tcgcccggtg gtcgaagcgg cccacgcaca
     5521 gaagatcaag gtgcgctgtg cgatctcggt cgcgctcggc tgcccgtacc agggcgaggt
     5581 cacggccgac gcggtcgagc gtgtcgtggt cctgatgaaa gcgatcgggg tcgaccattg
     5641 cggcatcgcc gacacgatag gcgtcggcac gccgcgccgc gcccgggccg cgctcgagcg
     5701 cgcgctcaag cacttcccgc tcgccgaggt cagcggccat ttccacgaca cctacggcca
     5761 ggcgctggcc aacgtctacg cctgcctcga gatgggcatc cataccttcg at
//
LOCUS       DASRDG010000320         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1654647, whole genome shotgun sequence.
ACCESSION   DASRDG010000320 DASRDG010000000
VERSION     DASRDG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654647"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1858
                     /locus_tag="VFA35_05320"
     CDS             <1..1858
                     /locus_tag="VFA35_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HZT55626.1"
                     /translation="GFGDGTAFLALQTAGDAAPDWSCDRRECFDARGRLVVPDEFGQR
                     SGAGLDPCALLATAIELPAGETAECIFLLGFGATPTDARQLATTAATVPAKQRLAASR
                     AMWDALLDAASVKTPDPLFDALVNRWLLYQTVACRLWAKAGFYQAGGAFGFRDQLQDA
                     MALAWGQPDMLRRQIVLNASRQFPEGDVQHWWHAPTGAGVRTHFADDLLWLPHACAHY
                     LRCTGDASLLDEPVAFIEGGPVPPGAEDAYEVPTVSGTSASVYEHGARALDHSLKVGA
                     HGLPLMGSGDWNDGMNRVGAEGRGESVWLGWFLCDLVDAYAPIARGRGETARARRWEE
                     AARKWREALQREAWDGQWYRRAFFDDGTPLGSSRNSECRIDLIAQAWAVISNAAPPAF
                     QRIAMHALDAQLVDADAGLIKLLTPALQTQQPSAGYIQAYPPGVRENGGQYSHAGVWA
                     LIAQAMRGDADTAYRYFTYLSPAHRAAHATRQRPYAIEPYVMAGDVYAEPPYVGRGGW
                     SWYTGSAAWMHRAAIEHLFGLQQRGGEVGFVPCLPSHWPQAELTLKRDGRSLRVIFCR
                     SGCEPAAIDAEITLAPGQMLRWAELGTHATALLRLEPTAPAETAAAAPATVP"
     gene            1994..2167
                     /locus_tag="VFA35_05325"
     CDS             1994..2167
                     /locus_tag="VFA35_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HZT55627.1"
                     /translation="MLKWALIFAVIAVVAGLFGFTGIAAGAAGIAKILFIVFLVLFVV
                     VVLLALLGIGAVT"
BASE COUNT          325 a          875 c          834 g          324 t
ORIGIN      
        1 cggcttcggc gacggcaccg cgttcctcgc gctgcagacc gccggcgacg cggcgcccga
       61 ctggagctgc gaccgccgcg agtgcttcga cgcgcgtggc cgcctggtcg tgcccgacga
      121 gttcggccag cgcagtggcg ccggcctcga cccctgcgcg ctgctcgcca ccgcgatcga
      181 gctgccggcg ggcgagacgg ccgagtgcat cttcctgctc ggcttcggcg cgacgccgac
      241 cgacgcgcgc cagctcgcga ccaccgccgc gaccgtgccg gcgaagcagc gcctggcggc
      301 cagccgtgcg atgtgggacg cgctgctcga cgccgcgagc gtcaagacgc ccgacccgct
      361 gttcgacgcg ctggtgaacc gctggctgct gtaccagacc gtcgcctgcc gcctgtgggc
      421 caaggccggc ttctaccagg ccggcggcgc gttcggcttc cgcgaccagc tgcaggatgc
      481 gatggcgctg gcttggggcc agcccgacat gctgcgccgc cagatcgtgc tgaacgcgtc
      541 gcgccagttc cccgagggcg acgtgcagca ctggtggcat gcgcccaccg gcgcgggcgt
      601 gcgaacccat ttcgccgacg acctgctgtg gctgccgcac gcctgcgcgc actacctgcg
      661 ctgcaccggc gacgcgagcc tgctcgacga gccggtcgcg ttcatcgagg gcggccccgt
      721 cccgcccggc gccgaagacg cctacgaggt gccgaccgtc agcggcacca gcgcgagcgt
      781 ctacgagcac ggcgcgcgcg cgctcgacca cagcttgaag gtcggcgcgc acggcctgcc
      841 gctgatgggc agcggcgact ggaacgacgg catgaaccgg gtcggcgccg aggggcgcgg
      901 cgagtcggtg tggctcgggt ggttcctgtg cgacctggtc gacgcctacg cgccgatcgc
      961 gcgcgggcgc ggcgaaaccg cgcgcgcgcg gcgctgggaa gaggcagcgc gcaagtggcg
     1021 cgaggcgctg cagcgcgagg cctgggacgg ccagtggtac cggcgcgcgt tcttcgacga
     1081 cggcacgccg ctcggctcga gccgcaacag cgagtgccgc atcgacctga tcgcgcaggc
     1141 ctgggccgtg atctcgaacg ctgcgccgcc ggcgttccag cgcatcgcga tgcacgcgct
     1201 cgacgcgcag ctggtcgacg ccgacgccgg cctgatcaag ctgctgacac cggcgctgca
     1261 gacgcagcag ccgagcgccg gctacatcca ggcctacccg cccggcgtgc gcgagaacgg
     1321 cggccagtat tcgcacgccg gcgtctgggc gctgatcgcg caggcgatgc gcggcgatgc
     1381 cgacaccgcc taccgctact tcacctacct gagcccggcg caccgcgccg cgcacgcaac
     1441 gcgccagcgg ccctacgcga tcgagcccta cgtgatggcc ggcgacgtat acgccgagcc
     1501 gccgtatgtc ggccgcggcg gctggagctg gtacaccggc tcggcggcgt ggatgcaccg
     1561 cgccgcgatc gaacatctgt tcggcctgca gcagcgcggc ggcgaggtcg gcttcgtgcc
     1621 gtgcctgccc tcgcactggc cccaggccga gctgacgctg aaacgcgatg ggcgcagcct
     1681 gcgcgtgatc ttctgccgca gcggctgcga gcccgcggcg atcgacgccg agatcacgct
     1741 cgcgccgggc cagatgctgc gctgggccga actcggcacc catgccaccg cgctgctgcg
     1801 gctcgagccc accgcgccgg ccgagactgc tgccgcagcg cccgcgaccg tgccctgaac
     1861 gctttcagtg cgtcgggatg ccgtcctaca ggggctggcg tgctgcgccg atgggcgccc
     1921 gaaccccagg ttcttacagt ccccccatcg accacagagg tgagcgcaag cgcccgcctc
     1981 ccaggagaaa accatgttga agtgggctct gatatttgcc gtgatcgccg tcgtcgcggg
     2041 cctgttcggc ttcaccggca tcgccgccgg cgcggccggc atcgcgaaga tcctgttcat
     2101 cgtcttcctc gtgctgttcg tcgtggtcgt gttgctggcc ctgctgggca tcggcgcggt
     2161 cacctgagcg aacgccgccg ttcatcgatc accgaatcac ccgaaggagc ccctcccatg
     2221 agcgcgaaga aagatgcccg caacgtcacg cagtttccga tcgacaccgg cgagcgcctg
     2281 gtgctcgatc cgcaaggcct ggcggacgcg aagaagtcgc tcgaccaggg cgcggtcgtg
     2341 ccgagcttcg gcccgtgg
//
LOCUS       DASRDG010000321         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_279883, whole genome shotgun sequence.
ACCESSION   DASRDG010000321 DASRDG010000000
VERSION     DASRDG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279883"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="VFA35_05330"
     CDS             <1..1463
                     /locus_tag="VFA35_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55628.1"
                     /translation="YRKDIFRNELAEKFKLDAFGEGGELETRQQWSGLAAEQLRLTVA
                     RLDPPRLQRVLLTNLERRYPGIAFIGLPELADEVDLNRITISARFKVPKLAAADNGNW
                     VMRFSPANMQGTIAVPPTPTRQFPLALPGFPMTVLYSAEMQWPASVSAALDPAHRRIA
                     NAAFNAEVTRSFRGNLATATLRFEPLTNSVPAKDVPALVTDARALERAIGGVMVVAPS
                     QVKDGGFLGIGRKTLQDNLLARANATVERTGKSIAGGQLGGDDLAQALCLRSEAQTEL
                     GNTAEALKDAQEAVRQAPSLGAAWFCQGNANWARADFAAASNDFGKALAFGAAASDVY
                     YRRGQARFFDGKLEPAADDFAKAVLDRPDARDKAYAQLWQAWTLQRLNRPLPPELSAA
                     AGADPTGAWPRPALAMFVGQMTPEQVIEQIGRKTGDDQELALAEGWFYIGEYQLNARQ
                     PDKARDAFEKARAKGITRYIEHAAAGFELQRLGARP"
     gene            complement(1482..1718)
                     /locus_tag="VFA35_05335"
     CDS             complement(1482..1718)
                     /locus_tag="VFA35_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZT55629.1"
                     /translation="MNVIDFALRGDHIALDALLKATGLAPSGGIAKAMVADGLVQVDG
                     RDELRKTCKIRAGQVVSLRGARVRVFAPEPGTSA"
     gene            1988..2197
                     /locus_tag="VFA35_05340"
     CDS             1988..2197
                     /locus_tag="VFA35_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZT55630.1"
                     /translation="MTTQTGTVKWFNESKGFGFIAQDAGGADLFAHFRDIQGNGFKTL
                     LENQRVEFEVKQGQKGLQAANIRVL"
     gene            2346..>3196
                     /locus_tag="VFA35_05345"
     CDS             2346..>3196
                     /locus_tag="VFA35_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZT55631.1"
                     /translation="MTSFKALTNHQIRLKARPNGLPTRADWEFTTEPVSEPTDGGVLV
                     KTLAISLDPAMRGWMNEGKSYIAPVALGEVMRAGGIGEVIASKNDAFKVGDMVSCTPG
                     VQEYALIPADQIKRNGLFKIDTRLGTPTQWLNVLGMSGMTAYFGLLDVGMPQPGQTVV
                     VSGAAGAVGQTVGQLAKIKGCRVVGIAGGPAKCAWVVKELGFDACVDYKAGDLKSALR
                     DACPKGVDVYFDNVGGEILDAALAQLAYKARVVICGAISQYNATGPIKGPSNYLALLV
                     ARASMTG"
BASE COUNT          549 a         1106 c         1089 g          452 t
ORIGIN      
        1 cgtaccgcaa ggacatcttc cgcaacgagc tcgccgagaa gttcaagctc gatgccttcg
       61 gcgaaggcgg cgaactcgag acccgccagc aatggagcgg cctggccgcc gagcagctgc
      121 gcctgacggt cgcacgcctc gacccgccgc gcctgcagcg cgtgctgctg acgaacctgg
      181 agcggcgcta ccccggcatc gccttcatcg gcctgcccga actcgccgac gaagtcgact
      241 tgaaccgcat cacgatcagc gcccggttca aggtgcccaa gctcgccgcc gccgacaacg
      301 gcaactgggt gatgcgcttc agccccgcca acatgcaggg cacgatcgcg gtgccgccga
      361 cgccgacgcg ccagttcccg ctcgcgctgc cgggcttccc gatgacggtg ctgtacagcg
      421 ccgagatgca gtggccggcg agcgtcagcg cggcacttga cccggcccac cgacgcatcg
      481 cgaacgccgc gttcaacgcc gaggtgacgc gcagcttccg cggcaacctc gcgaccgcga
      541 cgctgcgctt cgagccgctg accaacagcg tgccggccaa ggacgtgccc gcgctcgtca
      601 ccgacgcgcg ggcgctggag cgcgcgatcg gcggcgtgat ggtggtcgcg ccaagccagg
      661 tcaaggacgg cggcttcctc ggcatcggtc gcaagacgct gcaagacaac ctgctcgcac
      721 gcgcgaacgc gacggtcgag cgcaccggca agtcgatcgc gggcggccag ctcggcggcg
      781 acgacctcgc gcaggcgctg tgcctgcgct ccgaggcgca gaccgaactc ggcaacacgg
      841 ccgaggcgct gaaggacgcg caggaggcgg tgcgccaggc gccgtcgctc ggcgcggcct
      901 ggttctgcca gggcaacgcg aactgggcgc gcgcggactt cgcggccgcg tcgaacgact
      961 tcggcaaggc gctggcgttc ggcgcggcgg cgtccgatgt ctactaccgg cgcggccagg
     1021 cgcgcttctt cgacggcaag ctcgagccgg ccgccgacga cttcgcgaag gccgtgctcg
     1081 accggcccga cgcccgcgac aaggcctatg cgcagctctg gcaggcctgg acgctgcagc
     1141 gcctgaaccg cccgctgccg cccgagctga gcgccgctgc cggcgccgac ccgacaggcg
     1201 cttggccgcg cccggcgctg gcgatgttcg tggggcagat gacgcccgag caggtcatcg
     1261 agcagatcgg ccgcaagacg ggcgacgacc aggaactcgc cctggccgag ggctggttct
     1321 acatcggcga gtaccagttg aacgcgaggc agcccgacaa ggcgcgcgac gccttcgaga
     1381 aggcacgcgc caagggcatc acgcgctaca tcgagcacgc ggcggcgggc ttcgagttgc
     1441 agcgcctcgg cgcgcggccg tgagtccgaa gccccagggc atcaggccga ggtgccgggc
     1501 tccggtgcga acacgcgcac gcgcgcgccg cgcaggctga cgacctggcc cgcgcggatc
     1561 ttgcaggtct tgcgcaactc gtcgcggccg tcgacctgca ccaggccgtc ggcgaccatc
     1621 gccttcgcga tgccgccgct gggcgcgagc ccggtggcct tgagcagcgc gtcgagcgcg
     1681 atgtggtcgc cgcgcagcgc gaaatcgatg acgttcatgc gaggaggttc atgatgcgtg
     1741 tcgattctga accacagcgc gcagtgctgc cggaagggaa atgaaccagg cgccggcaca
     1801 tcacggcatt tcgtgtacag tccgccccac agttatgcgc aagacagtgg ttgctagaga
     1861 ttcttcccct tgccgacgcg gttctcgcgg cggtcgtcga tagaaatccc cctgatcgtt
     1921 tcttgagcgt cgctgcatgc gccgcatgtg ctgcgcatta ttcgcaaata gataggataa
     1981 gttctccatg acgacccaaa ccggtaccgt gaagtggttc aacgagagca agggcttcgg
     2041 cttcatcgct caggacgcgg gcggcgccga cctgttcgcc cacttccgtg acatccaggg
     2101 caatggcttc aagaccctgc tggaaaacca gcgcgtcgaa ttcgaagtca agcaaggcca
     2161 gaagggcctg caagccgcga acatccgcgt gctgtgagcc acgcggggca cgagccccgc
     2221 acacgaagaa gagccgcggc gatgccgcgg cttttttttg cccgcccgtg gcgtcgcatc
     2281 ggcgactttg caggcttgtc gcgcggccta agatctgttg ccgacctcgc cggagaaccc
     2341 aggccatgac ctcattcaaa gcgctgacca accaccagat ccgcctgaag gcgcgcccga
     2401 acggcctgcc cacgcgcgcc gactgggaat tcacgaccga gccggtcagc gagccgaccg
     2461 atggtggcgt gctcgtcaag acgctcgcga tctcgctcga cccggcgatg cgcggctgga
     2521 tgaacgaggg caagagctac atcgcgccgg tggcactcgg cgaggtgatg cgcgcgggcg
     2581 gcatcggcga ggtgatcgcg tcgaagaacg acgcgttcaa ggtcggcgac atggtcagct
     2641 gcacgccagg cgtgcaggag tacgccctga tcccggccga ccagatcaag cgcaacggcc
     2701 tgttcaagat cgacacccgc ctgggtacgc cgacgcagtg gctcaacgtg ctcggcatgt
     2761 ccggcatgac cgcgtacttc ggcctgctcg atgtcggcat gccgcagccc ggccagacag
     2821 tggtggtttc gggcgcggcc ggcgcggtcg gccagaccgt cggccagctc gcgaagatca
     2881 aaggctgccg cgtcgtcggc atcgcgggtg gcccggcgaa gtgcgcatgg gtcgtcaagg
     2941 aactcggctt cgacgcctgc gtcgattaca aggccggcga cttgaagagt gcgctgcgcg
     3001 acgcctgccc gaaaggcgtc gacgtgtact tcgacaacgt cggcggcgag atcctcgacg
     3061 cggcgctggc gcagctggcc tacaaggcgc gcgtcgtgat ctgcggcgcg atctcgcagt
     3121 acaacgcgac cggcccgatc aagggcccgt cgaactacct ggcgctgctc gtcgcccgcg
     3181 cgagcatgac cggcat
//
LOCUS       DASRDG010000322         3368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1201965, whole genome shotgun sequence.
ACCESSION   DASRDG010000322 DASRDG010000000
VERSION     DASRDG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201965"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1215)
                     /locus_tag="VFA35_05350"
     CDS             complement(190..1215)
                     /locus_tag="VFA35_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZT55632.1"
                     /translation="MTTPIQATLIPGDGIGPEIVDATLAVLDALKAPFVWDRQVAGLA
                     GVHASGDPLPQAALDSIRRTRLALKGPLETPSGGGYRSSNVRLREEFQLYANLRPART
                     LVPGGRFDKIDLVVVRENLEGLYIGHEHYVKIDDDPHAVAMATGINTRAGSRRLLEYA
                     FDTAVATGRKKVTIVHKANIMKALTGIFLETGLDLYERKYKGQFILDTVIIDACAMKL
                     VLNPWQFDMLVTTNLFGDILSDLVAGLVGGLGMAPGANIGTDAAIFEAVHGSAPDITG
                     KGLANPTAIMLAAALMLDHVKLPELGTRLRTAIDETLNIDKVRTGDLGGTASTAAFTK
                     ALVSRIG"
     gene            complement(1290..2498)
                     /locus_tag="VFA35_05355"
     CDS             complement(1290..2498)
                     /locus_tag="VFA35_05355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZT55633.1"
                     /translation="MKASVAVTGLGAVAPHGDDPQAMFAALWRGESALQPVFPELPKP
                     AAAATVAFDPARWFTKLQLAGVDRVSQLAVAAADLALRDAGTSTASFDAERIGVYAGC
                     GMGGAAALDTAYRSGGRVPPLTIPAFMPNAPAAHVAMRQGVLGPVLTYSVACASSAVA
                     IAEAAAAIERGDIDLALAGGTEALVVPGVVLAWQAMQTLASFEPGAAAHAVQPFAPGR
                     SGFALGEGAAFIVLESAERARRRGARICAQLAGWGLSSDATHLTKPDAPGQARALRAA
                     LRRAGLQPRDVGYCNAHGTATRIGDVVERDALALVWGDALDTLRVSSTKALHGHLLGA
                     AGALEAVITVLALQRGELPPNINGGEVDPACAMNLVLEPGTAARGLHAAISNSFAFGG
                     TNSVLLFTRA"
     gene            complement(2495..2734)
                     /locus_tag="VFA35_05360"
     CDS             complement(2495..2734)
                     /locus_tag="VFA35_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZT55634.1"
                     /translation="MSSLKELQDLIHEKFGIDPAELTPEASLREKGFDSLALVEFLFA
                     IEDHFKISMRDEDSNIDTLGQLAAAVDDIRAKQAA"
     gene            complement(2731..>3368)
                     /locus_tag="VFA35_05365"
     CDS             complement(2731..>3368)
                     /locus_tag="VFA35_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZT55635.1"
                     /translation="AWVWAFFIGYFWLLRNPAYPVTVMPLTALDHLIPFQPVTLVAYL
                     SLWFYVGIAPGLQLTFRELLVYGLWAGALCLAGLGLFYFWPTAVPPPGFDVSGHPGFK
                     MLQGVDAAGNACPSMHVAIALFTAVWIEHLLRGARAPAALRAVNVGWFLAIAWSTLAI
                     RQHVVIDVLAGGVLGLAFALASLRWRPRAAPRPVPQIGADIIERMSTDAAS"
BASE COUNT          484 a         1162 c         1243 g          479 t
ORIGIN      
        1 tggagcggca caggcgcgtt cgggctgggc gcgtcgcttc gcctcggcgg tcgatgctgc
       61 acatcgactg ctctgcggtg cgcggccacg tgccctgcgc tcctcgacgc ctcccacgaa
      121 gcgccacgcc cggcccgaac gcgcctttgc accaccgccg tggcacgcgc gaacagcgct
      181 ccgcaaatct cacccgatgc gactgaccag cgccttcgtg aacgcggccg tgctggccgt
      241 gccgcccagg tcgccggtgc gcaccttgtc gatgttgagc gtctcgtcga tcgcggtgcg
      301 cagccgcgtg ccgagctccg gcagcttgac gtggtcgagc atcagcgccg cggcgagcat
      361 gatcgccgtc gggttcgcca ggcccttgcc ggtgatgtcg ggcgccgagc cgtgcacggc
      421 ctcgaagatc gccgcatcgg tgccgatgtt cgcgcccggc gccatgccca ggccgccgac
      481 caggccggcg accaggtcgg acaggatgtc gccgaacagg ttggtcgtca ccagcatgtc
      541 gaactgccac gggttcagca ccagcttcat cgcgcaggcg tcgatgatca cggtgtcgag
      601 gatgaactgg cccttgtatt tgcgttcgta cagatcgagc ccggtctcga ggaagatgcc
      661 ggtcagcgcc ttcatgatgt tggccttgtg cacgatcgtg accttcttgc ggccggtcgc
      721 gaccgcggtg tcgaaggcgt attcgagcag gcggcggctg ccggcgcggg tgttgatgcc
      781 ggtggccatc gcgaccgcgt gcgggtcgtc gtcgatcttc acgtagtgct cgtggccgat
      841 gtacaggcct tcgaggttct cgcgcacgac caccaggtcg atcttgtcga agcgcccgcc
      901 gggcaccagc gtgcgcgccg ggcgcaggtt ggcgtagagc tggaactcct cgcgcaggcg
      961 cacgttcgac gagcggtagc cgccgcccga cggcgtttcg agcgggcctt tcagcgccag
     1021 ccgggtacgg cggatgctgt cgagcgcggc ctggggcagc gggtcgccgc tggcgtgcac
     1081 gccggcgagg ccggcgacct ggcggtccca gacgaacggg gctttcagcg cgtcgagcac
     1141 ggccagcgtc gcgtcgacga tttctgggcc gatgccgtcg ccagggatca gggtggcttg
     1201 gatgggggtt gtcatcgttg gctcgctcgt caaagaagtc agtgggtgtg atcggccgga
     1261 agcatacgct gtgcccgcgc tcggcgccct caggcgcggg tgaacagcag caccgagttc
     1321 gtgccgccga acgcgaacga gttgctgatc gcggcgtgca gtccgcgtgc ggccgtgccg
     1381 ggttcgagca cgaggttcat cgcgcaggcc gggtcgacct cgccgccgtt gatgttcggc
     1441 ggcagttcgc cgcgctgcag cgcgagcacc gtgatcaccg cttcgagcgc gccggccgcg
     1501 ccgagcaggt ggccgtgcag cgccttggtc gagctgacgc gcaaggtgtc gagcgcgtcg
     1561 ccccagacca gcgccagcgc atcgcgctcg accacgtcac cgatgcgcgt ggcggtgccg
     1621 tgtgcgttgc agtagccgac gtcgcgcggc tgcaggccgg cgcggcgcag cgcggcacgc
     1681 agcgcgcgcg cctggcccgg cgcgtcgggc ttggtcaggt gcgtggcgtc gctgctgagg
     1741 ccccagccgg cgagttgcgc gcagatgcgt gcgccgcgcc ggcgcgcgcg ctcggccgat
     1801 tcgagcacga tgaaggccgc gccttcgccg agcgcgaagc cgctgcggcc gggggcgaac
     1861 ggctgcaccg cgtgggcggc cgcgccgggc tcgaagctcg cgagggtctg catcgcctgc
     1921 cacgcgagca ccacgccggg cacgaccaga gcctctgtgc cgccggcgag cgccaggtcg
     1981 atgtcgccgc gttcgatcgc cgcggccgcc tcggcgatcg ccaccgccga cgatgcgcag
     2041 gcgaccgagt acgtcagcac cggaccgagc acgccttggc gcatcgcgac gtgcgccgcc
     2101 ggggcgttcg gcatgaaggc aggaatcgtc agcggcggca cgcggccgcc gctgcggtag
     2161 gcggtatcga gcgccgcggc gccgcccatg ccgcaaccgg cgtagacgcc gatccgttcg
     2221 gcgtcgaacg acgcggtcga ggtgccggca tcgcgcagcg ccaggtcggc ggccgccacc
     2281 gcgagctggc tgacgcggtc gacgccggcg agctgcagct tcgtgaacca gcgtgcgggg
     2341 tcgaacgcga cggtggccgc cgcggcgggc ttgggcaact cgggaaagac cggctgcagc
     2401 gccgattcgc cgcgccacag cgccgcgaac atcgcctgcg gatcgtcgcc gtgcggcgcg
     2461 acggcgccca ggccggtgac cgcgaccgag gctttcacgc cgcctgcttg gcccggatgt
     2521 cgtcgaccgc ggcggccagc tggccgagcg tgtcgatgtt cgagtcttcg tcgcgcatcg
     2581 agatcttgaa gtggtcttcg atcgcgaaca ggaattcgac cagcgcgagc gaatcgaagc
     2641 ccttctcgcg cagcgacgcc tcgggcgtga gctcggccgg gtcgatgccg aacttctcgt
     2701 ggatcaggtc ttgcagttcc ttcagcgagc tcatgatgcg gcgtccgtcg acatgcgttc
     2761 gatgatatct gctccgatct gcggcaccgg gcgcggcgcg gcccgcggtc gccagcgcag
     2821 cgaggccagc gcgaacgcca gcccgagcac gccgccggcc agcacgtcga tcacgacgtg
     2881 ctggcggatc gccagcgtcg accaggcgat cgcgaggaac cacccgacgt tgacggcgcg
     2941 cagcgccgcc ggcgcgcggg cgccgcgcag caggtgctcg atccagaccg ccgtgaacag
     3001 cgcgatcgcg acgtgcatcg acgggcacgc gttgccggcg gcgtccacgc cctgcagcat
     3061 cttgaagccg gggtggcccg agacgtcgaa gcccggcggc ggcacggcgg tcggccagaa
     3121 gtagaacagg ccgaggccgg ccaggcacag cgcgccggcc cacagcccgt acaccagcaa
     3181 ctcgcggaag gtcagctgca aacccggcgc gatgccgacg tagaaccaca gcgagaggta
     3241 ggcgaccagc gtgacgggct ggaacgggat caggtggtcg agcgcggtca gcggcatcac
     3301 cgtgaccggg tacgccgggt tgcgcagcag ccagaagtag ccgatgaaga aggcccagac
     3361 ccaggcgg
//
LOCUS       DASRDG010000323         5052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1432631, whole genome shotgun sequence.
ACCESSION   DASRDG010000323 DASRDG010000000
VERSION     DASRDG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432631"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..717)
                     /locus_tag="VFA35_05370"
     CDS             complement(76..717)
                     /locus_tag="VFA35_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZT55636.1"
                     /translation="MSSTPAEPPSPLAGGLPLFPLQTPLFPGGRLGLTVFEARYLDLI
                     AACLRSRQPFGVVALRSGGEVRRSGEAVSFEPVGTLAELISADCERPGILLAQCRGTT
                     RFRVEASRQQADGLWLAQAGAIDDDEAVAPDASLLDTVRALAATIPTLKNSKNESPFL
                     PPYRFDDAGWVANRWCEILPISQAAKQKLMELPDPMVRLELVDDFLRSKGVVK"
     gene            complement(698..1600)
                     /gene="rapZ"
                     /locus_tag="VFA35_05375"
     CDS             complement(698..1600)
                     /gene="rapZ"
                     /locus_tag="VFA35_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZT55637.1"
                     /translation="MSNAPVPATPSPREIVLVTGISGSGKSVALHALEDAGFFCVDNL
                     PPELLREFLRLEHGRQDRRLAIAVDVRSAGSLPHLLPLIQQLRAEGTGIQVIFLDAST
                     EALVRRFSETRRPHPLSAEVSSDEPDGRHALVDAIEIERGLLAELRELASVVDTSQLR
                     PAQLRIWVRDMVRAGGNRLTLVFESFAFKHGVPLDADYVFDVRVLPNPHYVHELRPLT
                     GRDVPVAEYLRAQPEVGEMLGQIEAFIARWLPAFERDQRSYLTVAIGCTGGQHRSVYF
                     VQTLAERFRARSATLVRHRELDAR"
     gene            complement(1597..3294)
                     /gene="recN"
                     /locus_tag="VFA35_05380"
     CDS             complement(1597..3294)
                     /gene="recN"
                     /locus_tag="VFA35_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZT55638.1"
                     /translation="MLRRLSLRDFVIVAELEVEFDAGFSVLTGETGAGKSILVDALQL
                     ALGSRGDAGVVREGAARAEVGAEFDTPASLARWLDDAGFDAGETMLLRRTIDAQGKGR
                     AWINGSAATIAQLREAADHLVDIHGQHAWQSLTRGPSVRALLDAFAGVDAAPLAALWS
                     HWKRAAEALAAAGTRRAELERERERLVWQIGELDKLAPGEGEWAELEAEHKKLANAQS
                     LIDAARSALDALADADSNAEALTGRALHALESVASFDAQLAALIDVLQGAQAQLQDAA
                     HTLSGYLNHTDLEPQRLQALDERLSAWLSLARRYRRPADELPALLAQWKDALRALDAA
                     ADLDGLKRELARALAAFEAEAARVSAARRAGARQLASAVTQAMQQLGMAGGRFEVALL
                     TQDAPQSFGVESAEFHVAGHAGSTPRPLGKVASGGELSRIALAIAVTTSRLKTRDDAT
                     AATLIFDEIDAGVGGAVAETVGRLMKQLGRDRQVLAVTHLPQVAACADHHFVVSKRSR
                     DGLTLSDVNAVSGELRVAEVARMLGGERLSNTSLAHAQEMLAVGADAAPAARGRKRS"
     gene            complement(3317..4192)
                     /locus_tag="VFA35_05385"
     CDS             complement(3317..4192)
                     /locus_tag="VFA35_05385"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HZT55639.1"
                     /translation="MSSPFRHAALVGKYQAQGIRGELEGIAQFLVRQGLEVSLERQTA
                     LNTGIEGFDALTVDELGRNCDLAVVVGGDGTMLGIARQLARFNTPLVGINQGRLGFIT
                     DINSAQFAETLAPMIAGDYEEEHRTMLEGDVWRSGVSIFESFAMNDVVVSRGAAASLV
                     ELKVDIGAEFVANFRSDGLIIGSPTGSTAYALSAGGPILHPGIAGWVLVPIAPHDLSN
                     RPIVLPDTGEVSIEIVAGRDASVSFDMQSLASLLHGDRIRVRRSANQVRFLHPRGWSY
                     YATLRRKLHWNAGMN"
     gene            4263..>5052
                     /gene="hrcA"
                     /locus_tag="VFA35_05390"
     CDS             4263..>5052
                     /gene="hrcA"
                     /locus_tag="VFA35_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZT55640.1"
                     /translation="MLDERAKTLLKALVERYIADGQPVGSRTLSRASGLELSPATIRN
                     VMADLEDLGLIASPHTSAGRIPTARGYRLFVDTMLTAQPLAGVPSVPPQLPHDQPQRV
                     IANAAQLLSNLSSFVGVITAPRKASVFHHIEFMRLSERRVLVILVAPDGDVQNRVIFT
                     AQDYTQSQLVEASNHLTANYRGLTIEAVRERLKTEVDALRGEIATLMHAAVQAGTEAH
                     ADSQEQVVISGERNLLAVRDFSSDMGSLRKLFDVFEHKTQLMRLL"
BASE COUNT          727 a         1831 c         1778 g          716 t
ORIGIN      
        1 tcggcttcga cgaagcgctc gccgccgggc tgccgtcgcc gctgcgcaag ctgctgctcg
       61 cgtcgcctca atcgatcact tgaccacgcc cttgctacgc agaaagtcgt cgacgagctc
      121 gagccgcacc atcgggtcgg gcagctccat cagcttctgt ttcgcggcct gcgagatcgg
      181 gaggatctcg caccagcggt tcgcgaccca gccggcgtcg tcgaagcggt agggtggcag
      241 gaacggcgac tcgttcttgc tgttcttcag cgtcgggatc gtcgcggcca gcgcgcgcac
      301 cgtgtcgagc agcgatgcgt cgggcgccac ggcttcgtcg tcgtcgatcg cgccggcctg
      361 cgccagccac aggccgtcgg cctgctgccg gctcgcctcg acgcgaaacc gcgtcgtccc
      421 gcggcactgc gcgagcagga tgccgggccg ctcgcagtcg gcgctgatca gctcggccag
      481 cgtgcccacc ggctcgaagc tcaccgcctc gccgctgcgc cggacctcgc cgccgctgcg
      541 cagcgcgacc acgccaaagg gctggcggct gcgcaggcac gcggcgatca ggtcgaggta
      601 gcgcgcctcg aacactgtca ggccgagccg cccgccgggg aacagcggcg tttgcagcgg
      661 gaacagcggc agtccgcccg ccagcggcga gggcggctca gcgggcgtcg agctcacggt
      721 ggcgcaccag cgtggccgag cgcgcgcgaa agcgctcggc gagcgtctgc acgaagtaca
      781 ccgagcggtg ctgaccgccg gtgcagccga tcgcgaccgt gaggtagctg cgctggtcgc
      841 gctcgaacgc gggcagccag cgcgcgatga aggcctcgat ctggcccagc atctcgccga
      901 cctcgggttg cgcgcgcagg tactcggcca ccggcacgtc gcggccggtc agcgggcgca
      961 gctcatgcac gtaatgcggg ttcggcaaca cacgcacgtc gaacacgtag tcggcatcga
     1021 gcggcacgcc gtgcttgaac gcgaagctct cgaacaccag cgtcagccgg ttgccgccgg
     1081 cgcgcaccat gtcgcgcacc cagatgcgca gctgcgccgg gcgcagctgg ctggtgtcga
     1141 cgacgctggc gagctcacgc agctcggcca gcaggccgcg ctcgatctcg atcgcgtcga
     1201 ccagcgcgtg gcgaccgtcg ggctcgtcgc tgctcacctc ggccgacagc gggtgcggcc
     1261 ggcgcgtttc ggaaaaacgc cgcaccagtg cctcggtgct cgcgtcgaga aagatcacct
     1321 ggatgcccgt gccctcggcg cgcagctgtt ggatcagcgg cagcaggtgc ggcagcgagc
     1381 cggcgctgcg cacgtcgacc gcgatcgcga ggcgccggtc ctggcgccca tgttcgagcc
     1441 gcaggaactc gcgcagcagt tcgggcggca ggttgtcgac acagaagaag cccgcgtctt
     1501 cgagcgcgtg cagcgcgacc gacttgccgg agccggatat cccggtcacc agcacgatct
     1561 cgcgcggcga gggcgtcgca ggaacgggtg cgttgctcat gaccgtttgc gcccgcgtgc
     1621 cgccggcgct gcatcggcgc cgaccgcgag catctcctgc gcgtgcgcga ggctggtgtt
     1681 cgacaggcgt tcgccgccga gcatgcgcgc gacctcggcg acccgcagct cgccgctgac
     1741 cgcgttcaca tcgctgagcg tgaggccgtc gcgcgaccgc ttcgagacga cgaagtggtg
     1801 gtcggcgcag gccgcgacct gcggcaggtg cgtgaccgcg agcacctgcc ggtcgcggcc
     1861 gagctgcttc atcaggcggc cgaccgtctc ggcgacggcg ccgccgacgc cggcgtcgat
     1921 ctcgtcgaag atcagcgtcg ccgcggtggc atcgtcgcgg gtcttcagcc ggctcgtcgt
     1981 caccgcgatc gccagcgcga tgcgcgacag ctcgccgccc gaggcgacct tgcccagcgg
     2041 ccgcggcgtg ctgcccgcgt gcccggcgac gtggaactcg gccgactcca cgccgaacga
     2101 ctgcggcgcg tcctgcgtca gcagcgcgac ctcgaagcgg ccgcccgcca tgccgagctg
     2161 ctgcatcgcc tgcgtgaccg cgctcgcgag ctggcgcgcg ccggcgcggc gcgcggccga
     2221 gacgcgcgcg gcctcggcct cgaaggcggc gagcgcgcgc gcgagttcgc gcttcaggcc
     2281 gtcgagatcg gccgccgcat cgagcgcgcg caacgcgtcc ttccattgcg cgagcagcgc
     2341 cggcagttcg tcggccggcc ggcgatagcg ccgcgccagg ctcagccagg ccgacaggcg
     2401 ttcgtcgagc gcctgcaggc gctgcggctc cagatcggtg tggttcaggt agccgctcag
     2461 cgtgtgcgcc gcgtcctgca actgcgcctg cgcgccttgc agcacgtcga tcagcgcggc
     2521 gagctgcgcg tcgaacgacg cgacgctttc gagcgcatgc agcgcgcggc cggtcagcgc
     2581 ttcggcgttg ctgtcggcat cggcgagcgc atcgagcgcg ctgcgcgccg cgtcgatcag
     2641 cgactgcgcg ttggccagct tcttgtgctc ggcctcgagc tcggcccatt cgccctcgcc
     2701 gggcgcgagc ttgtcgagct cgccgatctg ccacacgagg cgctcgcgtt cgcgctcgag
     2761 ctcggcccgg cgcgtgccgg ccgcggcgag cgcctcggcg gcgcgcttcc agtgcgacca
     2821 cagcgcggcg agcggcgcgg catcgacgcc ggcgaacgcg tcgagcagcg cgcgcaccga
     2881 cggcccacgc gtcaggctct gccaggcgtg ctggccgtgg atgtcgacga ggtggtcggc
     2941 ggcctcgcgc agctgcgcga tcgtcgcggc gctgccgttg atccaggcgc ggcctttgcc
     3001 ctgcgcatcg atggtccggc gcagcagcat ggtctcgccg gcatcgaagc cggcgtcgtc
     3061 gagccagcgc gccaggctcg ccggcgtgtc gaactcggcg ccgacctcgg cgcgcgccgc
     3121 accttcgcgc accacgccag catcgccgcg gctgccgagc gcgagctgca gtgcgtcgac
     3181 caggatcgac ttgcccgcgc cggtctcgcc ggtcagcacc gagaagccgg catcgaactc
     3241 gacttcgagt tcggcgacga tgacgaagtc gcgcagtgac aagcggcgca gcatgttcaa
     3301 gcctccgggc gcctcatcag ttcattccgg cgttccagtg cagcttgcgc cgcagcgtgg
     3361 cgtagtagct ccagccgcgc ggatgcagga agcgcacctg gttcgccgag cgccgcacgc
     3421 ggatgcggtc gccgtgcagc aggctcgcga gcgactgcat gtcgaagctg acgctggcgt
     3481 cgcgcccggc gacgatctcg atgctgactt cgccggtgtc gggcagcacg atcggccggt
     3541 tcgacaggtc gtgcggcgcg atcggcacca gcacccagcc ggcgatgccc gggtgcagga
     3601 tcggcccgcc ggccgagagc gcgtacgcgg tcgagccggt gggcgagccg atgatcaggc
     3661 cgtcggagcg gaagttcgcg acgaactcgg cgccgatgtc gaccttcagc tcgaccaggc
     3721 tggcggccgc gccgcggctg acgaccacgt cgttcatcgc gaagctctcg aagatcgaga
     3781 ccccgctgcg ccagacatcg ccttcgagca tcgtgcggtg ctcttcctcg tagtcgccgg
     3841 cgatcatcgg cgcgagtgtc tcggcgaact gcgcgctgtt gatgtccgta atgaacccga
     3901 ggcgcccctg gttgatgccg accagcggcg tgttgaagcg ggccagctgg cgcgcgatgc
     3961 cgagcatcgt gccgtcgccg ccgacgacga ccgcgaggtc gcagttgcgg ccgagttcgt
     4021 cgaccgtcag cgcgtcgaag ccctcgatgc cggtgttcag cgcggtctgg cgttcgagcg
     4081 agacctcgag gccctggcgc acgaggaact gcgcgatccc ttcgagctcg ccgcggatgc
     4141 cctgcgcctg gtactttccc acgagcgcgg catgacgaaa ggggctggac atgcgcggaa
     4201 ttacaccaca gcgatcctcc atggccgcgg ccccgaatga cccgccgcct acaatgagaa
     4261 acatgctgga cgaacgcgcc aagacgctgc tcaaagccct ggtcgaacgc tacatcgccg
     4321 acggccaacc ggtgggctcg cgcacgcttt cgcgcgccag cgggctggag ctttcgcccg
     4381 cgacgatccg caacgtgatg gccgacctcg aagacctcgg cctgatcgcc agcccgcaca
     4441 ccagcgccgg gcgcatcccg accgcgcgcg gctatcgcct gttcgtcgac acgatgctga
     4501 ccgcgcagcc gctcgccggc gtgcccagcg tgccgccgca actgccgcac gaccagcccc
     4561 agcgcgtgat cgcgaacgcg gcgcagctgc tgtcgaacct gtccagcttc gtcggcgtga
     4621 tcaccgcgcc gcgcaaggcg agcgtgttcc accacatcga gttcatgcgt ctatcggagc
     4681 ggcgcgtgct ggtgatcctg gtcgcgcccg acggcgacgt gcagaaccgc gtcatcttca
     4741 ccgcccagga ctacacccag tcgcaactcg tcgaggccag caaccacctg accgcgaact
     4801 accgcggtct gacgatcgag gcggtgcgcg agcgcctgaa gaccgaggtc gatgcgctgc
     4861 gcggcgagat cgccacgctg atgcacgccg cggtgcaggc cggcaccgag gcccacgccg
     4921 acagccagga gcaggtcgtg atcagcggcg agcgcaacct gctcgcggtg cgcgactttt
     4981 cgagcgacat ggggagcctg cgcaagctgt tcgacgtgtt cgagcacaag acccagctga
     5041 tgcgcctgct cg
//
LOCUS       DASRDG010000324         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_51344, whole genome shotgun sequence.
ACCESSION   DASRDG010000324 DASRDG010000000
VERSION     DASRDG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51344"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFA35_05395"
     CDS             <1..726
                     /locus_tag="VFA35_05395"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZT55641.1"
                     /translation="TTDRAAVGLLIASPEAVDVIIPRGGKGLIERIAREAKVPVIKHL
                     DGNCHTYVDEHVDLALAVKVTDNAKPQKYSPCNATESLLVHADVAAAFLPRIGALFAA
                     KGVEMRGDPAALALLQDVPNAKLAAATEQDWAEEYLAPIISVKVVTGLDEAIAHINRF
                     GSHHTDAILTESHANAQRFLREVDSASVMVNASTRFADGFEYGLGAEIGISTDKFHAR
                     GPVGLEGLTSMKWVVLGHGETRG"
     gene            707..1447
                     /gene="queC"
                     /locus_tag="VFA35_05400"
     CDS             707..1447
                     /gene="queC"
                     /locus_tag="VFA35_05400"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZT55642.1"
                     /translation="MARHVADARRALVLFSGGQDSTACLAWALERYAQVETIGFDYGQ
                     RHRVELDCRLQVRAALHAQFPRWARKLGDDHLLDLALLGQISDTALTDTRAIEMTAQG
                     LPNTFVPGRNLMFFNFAAAVAYRRGASVLVGGMCETDFSGYPDCRDNTLRALQVAISL
                     GLDAPMTIETPLMWLDKAQTWALTEQLGGAPLTELVVEHTHTCYLGDRTQRHAWGYGC
                     GRCPACELRRKGFEGWLGRARSDVESAP"
     gene            1444..1971
                     /locus_tag="VFA35_05405"
     CDS             1444..1971
                     /locus_tag="VFA35_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZT55643.1"
                     /translation="MTTTQIVGCVLAAVLLFWAVGAYNRLVRLRAAIVRQFAPVDAQF
                     RARHALLLQLTDALAPVLTNAASRIEALRAACGQVEAACAQAKLRPGATGKITSLRLA
                     DEILAEARARLPVQSAPGSELAALNAQLQASDATLAFARRQFDAAVIDYNHAVQQFPT
                     LLLVGLFGFRPAATL"
BASE COUNT          300 a          730 c          705 g          289 t
ORIGIN      
        1 accaccgacc gcgccgcggt cgggttgctg atcgcgtcgc ccgaggcggt cgacgtgatc
       61 atcccgcgcg gcggcaaggg tctgatcgag cgcatcgcgc gcgaggccaa ggtgccggtg
      121 atcaagcacc tggacggcaa ctgccatacc tatgtcgacg agcacgtcga cctcgcgctc
      181 gcggtgaagg tgaccgacaa cgcgaagccg cagaagtaca gcccgtgcaa cgcgaccgag
      241 tcgctgctgg tgcacgccga cgtcgcagcc gcgttcctgc cgcgcatcgg cgcgttgttc
      301 gcggccaagg gcgtcgagat gcgcggcgac ccggccgcgc tcgcgctgct gcaagacgtg
      361 ccgaacgcga agctcgccgc cgcgaccgag caggactggg ccgaggaata cctcgcgccg
      421 atcatcagcg tgaaggtcgt caccggcctc gacgaggcga tcgcgcacat caaccgcttt
      481 gggtcgcacc acaccgacgc gatcctgacc gagtcgcacg cgaacgcgca gcgcttcctg
      541 cgcgaggtcg attcggccag cgtgatggtc aacgcctcga cccgctttgc cgacggcttc
      601 gagtacggcc tgggcgccga gatcggcatc agcaccgaca agttccatgc gcgcgggccg
      661 gtcgggctcg aaggcctgac ctcgatgaag tgggtcgtgc tggggcatgg cgagacacgt
      721 ggctgatgca cgccgcgcgc tggtgctgtt ctccggcggt caggattcca cagcctgtct
      781 cgcgtgggcg ctcgagcgct acgcgcaagt cgagacgatc ggcttcgact acggccagcg
      841 ccaccgcgtc gagctcgact gccggctgca ggtgcgcgcg gcgctgcacg cgcagttccc
      901 gcgctgggcg cgcaagctcg gcgacgacca cctgctcgac ctcgcgctgc tcggccagat
      961 ctccgacacc gcgctgaccg acacgcgcgc gatcgagatg accgcgcagg gcctgcccaa
     1021 caccttcgtg ccgggccgca acctgatgtt cttcaacttc gccgcggccg tggcctaccg
     1081 gcgcggcgcg agcgtgctgg tcggcggcat gtgcgagacc gacttttccg gttaccccga
     1141 ttgccgcgac aacacgctgc gcgcactgca ggtcgcgatc agcctcgggc tcgatgcgcc
     1201 gatgacgatc gagacgccgc tgatgtggct cgacaaggcg cagacctggg cgctgaccga
     1261 gcagctcggc ggcgcgccgc tgaccgagct cgtcgtcgag cacacccaca cctgctacct
     1321 gggcgaccgc acgcagcgcc acgcctgggg ctacggctgc ggccggtgcc cggcctgcga
     1381 gctgcggcgc aagggcttcg aggggtggct cgggcgcgcc cgatccgacg tggagtccgc
     1441 cccatgacca cgacccaaat cgtcggctgc gtgctcgccg cggtgctgct gttctgggcc
     1501 gtcggcgcct acaaccggct ggtgcgcttg cgcgccgcga tcgtgcgtca gttcgcgccg
     1561 gtcgacgcgc agttccgcgc ccgccatgcg ctgctgctgc agctgaccga cgcgctcgcg
     1621 ccggtgctga cgaacgccgc gtcgcgcatc gaggcgctgc gcgcggcctg cgggcaggtc
     1681 gaggcggcct gcgcgcaagc caagctgcgt ccgggcgcaa ccggcaagat cacgagcctg
     1741 cggctggccg acgagatcct cgccgaggcg cgcgcgcgcc tgccggtgca gagcgcgccc
     1801 ggcagcgagc tcgcggcgct gaacgcgcag ttgcaagcca gcgacgcgac gctcgcgttc
     1861 gcgcgccgcc agttcgacgc cgcggtgatc gactacaacc atgcggtgca gcagttcccg
     1921 acgctgctgc tggtggggct gttcgggttc cgccccgccg cgaccctctg aaacagcgcg
     1981 acactgcggc gtcagcgcac aggaggttcc cgatgaagct cgtc
//
LOCUS       DASRDG010000325         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1562078, whole genome shotgun sequence.
ACCESSION   DASRDG010000325 DASRDG010000000
VERSION     DASRDG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562078"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFA35_05410"
     CDS             <1..411
                     /locus_tag="VFA35_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HZT55644.1"
                     /translation="VGLNLWAWHQRNAIDAKQAAMQSLVKATYPNVNPLDVQRDADAV
                     MQRETQALRTLAGKPGDADLEPMLQAAASAWPADRPPVDTMRFEPGRLTLTAPGWSDA
                     QIAQFRSLLRPSGWAVEASEGRLTLSRARPGASS"
     gene            408..926
                     /gene="gspM"
                     /locus_tag="VFA35_05415"
     CDS             408..926
                     /gene="gspM"
                     /locus_tag="VFA35_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HZT55645.1"
                     /translation="MNLGSTASAPAMASLRQQAGTWWGARTPRERQAVALVAAILGAF
                     IVWSLLVQPALTTARTAPAQLDQLDAQYQQMQRIATESSALRAAPRVAPGQAQLALKA
                     ATDRLGERGRLVVQGDRATLTLTTGVSPEALRAWLAEARSGARTRPVEAQLQRGALGY
                     TGSIGVTLGAGP"
     gene            923..1816
                     /gene="gspN"
                     /locus_tag="VFA35_05420"
     CDS             923..1816
                     /gene="gspN"
                     /locus_tag="VFA35_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HZT55646.1"
                     /translation="MIRLRRKRTRGLFAPTVAATGWGESTLAEISWDKSRGAARRWGV
                     AGAAVGALVALIAFAPAAWLAGRVASTTGQRLLLADARGTVWNGSAVAVLTGGEGSRD
                     ASALPGRLSWTLRPRGLGLELQLQHACCLNGTVALQVRPGFGRTEYTLVPPAGWVGQW
                     PAALLGGLGTPFNTMQLGGSVRLESKGLRFESVQGRLIVDGRADFDIIEASSRLSTLP
                     SLGSYRMSVSGDAANPGISLLSLSTGGGALQLSGSGTWGPAGVRFRGEARASVGEEAA
                     LSNLLNIIGRRDGARSVISIG"
     gene            1819..>2999
                     /locus_tag="VFA35_05425"
     CDS             1819..>2999
                     /locus_tag="VFA35_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZT55647.1"
                     /translation="MKPSPQLKHRALPAAVAAAVLLAALAPAPLAFAQQGTKVPRAAR
                     GGETVTLNFVNAEIDGVARAMGAILKQQFVVDPRVKGTMTLYSDTPLTPRAAYSNFLA
                     TLRGLGFTVVEVGGLYKIVPEADAKLQTGTVSVDDKNRRGGDQVITQIFRLTHENANN
                     LVGVLRPLISPNNTINANPGNNTLVITDYADNLQRIGKIIAAMDTPASGDVEVIALKY
                     AVAADIAPLVQRMTDSGGAGGAGAAPTGAAGAGATSVQVDARSNTLLVRAPNPARMAS
                     IRALIDKLDKPVQSAGAAGNVWVVYLRNADAVKLAQVLRAAYGGAAGGGGAGSGGGYA
                     SGGSTQPASPLTTTGSANTGASTQTTAPLSTSAGPSTGGFVQADPASNALIITAPEPL
                     YR"
BASE COUNT          421 a         1101 c         1117 g          360 t
ORIGIN      
        1 gtcggcctga acctgtgggc ctggcaccag cgcaacgcga tcgacgcgaa gcaggcggcg
       61 atgcaaagcc tggtcaaggc gacctacccg aacgtcaacc cgctggacgt gcagcgcgac
      121 gccgacgcgg tgatgcagcg cgagacgcaa gcgctgcgca cgctcgccgg caagcccggc
      181 gacgccgacc tcgagccgat gctgcaggcc gccgcctcgg cctggccggc cgaccgcccg
      241 ccggtcgaca cgatgcgctt cgagcccggc cggctgacac tcaccgcccc gggctggagc
      301 gacgcgcaga tcgcgcagtt ccgcagcctg ctgcgcccca gcggctgggc tgtcgaggcg
      361 agcgaggggc gcctgacgct gagccgcgcg cggccgggag cgagttcgtg aacctcggca
      421 gcaccgcttc tgcccccgcg atggcgagcc tgcgccagca ggccggcacc tggtggggcg
      481 cgcgcacgcc gcgcgagcgc caggcggtcg cgctggtcgc ggccatcctc ggcgccttca
      541 tcgtctggag cctgctcgtg cagcccgcgc tgaccaccgc gcgcaccgcg ccggcgcagc
      601 tcgaccagct cgacgcgcag taccagcaga tgcagcgcat cgcgaccgag agcagcgcgc
      661 tgcgcgccgc gccgcgggtc gcgccgggcc aggcgcagct cgcgctgaag gcggcaaccg
      721 accggctcgg cgagcgcggc cggctcgtgg tgcagggtga tcgcgcgacg ctgacgctca
      781 cgaccggcgt cagccccgag gccttgcgcg cctggctcgc cgaggcgcgc agcggcgcgc
      841 gcacgcgccc ggtcgaggcg cagttgcagc gcggcgcgct cggctacacc ggcagcatcg
      901 gcgtgacatt gggggctggc ccgtgatccg gctgcgccgc aagcgcacgc gcgggctgtt
      961 cgcgccgacc gtcgccgcga ccggctgggg cgaatcgacc ctcgccgaga tctcgtggga
     1021 caagtcgcgc ggcgcggcgc ggcgctgggg ggtggccggc gccgccgtcg gcgcgctcgt
     1081 cgcgctgatc gcgttcgcgc cggcggcctg gctcgccggg cgcgtcgcgt cgacgaccgg
     1141 gcagcgcctg ctgctcgccg acgcccgcgg caccgtctgg aacggcagcg cggtcgcggt
     1201 gctcaccggc ggcgaaggca gccgcgacgc gagcgcgctg cccggccgcc tgagctggac
     1261 gctgcgcccg aggggcctcg gcctcgagct gcagctgcaa catgcgtgct gcctgaacgg
     1321 cacggtggcg ctgcaggtgc ggcctggctt cggccgcacc gagtacacgc tggtgccgcc
     1381 ggccggctgg gtcggccagt ggccggcggc gctgcttggc gggctgggca cgccgttcaa
     1441 cacgatgcag ctcggcggct ccgtgcggct cgagtcgaag gggctgcggt tcgagtcggt
     1501 gcagggccgg ctgatcgtcg atggccgggc cgatttcgac atcatcgaag cctcgtcgcg
     1561 gctgagcacc ctgccctcgc tcggcagcta ccgcatgagc gtctcgggcg acgccgccaa
     1621 ccccggcatc tcgctgctct cgctgagcac cggcggcggc gcgctgcagc tcagcggcag
     1681 cggcacctgg ggtccggccg gcgtgcgctt tcgcggcgaa gcccgcgcca gcgtcggcga
     1741 agaggcggcg ctgtcgaacc tgctgaacat catcgggcgg cgcgacggcg cccgctccgt
     1801 catttcgatc ggatgagcat gaagccaagc ccccaactca aacaccgcgc gctgccggct
     1861 gcggtcgcgg ccgcggtgct actggccgcg ctggcgccgg caccgctcgc gttcgcgcaa
     1921 cagggcacga aggtgccgcg tgcggcgcgc ggcggcgaaa ccgtgacgct gaacttcgtc
     1981 aatgccgaga tcgacggcgt ggcgcgcgca atgggcgcga tcctgaagca gcagttcgtc
     2041 gtcgacccgc gcgtcaaggg cacgatgacg ctgtacagcg acacgccgct gacgccgcgc
     2101 gcggcctaca gcaactttct cgcgacgctg cgcggcctgg gcttcacggt cgtcgaggtc
     2161 ggcggcctgt acaagatcgt tccggaggcc gatgcgaagc tgcagaccgg cacggtcagc
     2221 gtcgacgaca agaaccgccg cggcggcgac caggtcatca cccagatctt caggctgacg
     2281 cacgagaacg cgaacaacct ggtcggcgtg ctgcgcccgc tgatcagccc gaacaacacg
     2341 atcaacgcga accccggcaa caacacactg gtgatcaccg actacgccga caacctgcag
     2401 cgcatcggca agatcatcgc ggcgatggac acgccggcct cgggcgacgt ggaagtgatc
     2461 gcgctgaagt acgcagtggc ggccgacatc gcgccgctgg tgcagcgcat gaccgacagc
     2521 ggcggtgccg gcggcgcggg cgcggcgccg acgggcgcgg ccggcgcggg cgcgacgtcg
     2581 gtgcaggtcg acgcgcgcag caacacgctg ctggtgcgcg cgcccaaccc ggcgcgcatg
     2641 gcgtcgatcc gcgcgctgat cgacaagctc gacaagccgg tgcagtcggc cggcgcggcg
     2701 ggcaacgtgt gggtcgtgta cctgcgcaat gccgacgcgg tgaagcttgc gcaggtgctg
     2761 cgtgcggcct atggtggcgc tgcgggcggc ggtggtgcag gcagcggcgg cgggtacgcg
     2821 tccggcggca gcactcagcc ggcatcgccg ctgaccacca ccggcagcgc gaacaccggc
     2881 gcctcgaccc agacgaccgc gccgctgtcg acctcggcgg gcccgtcgac cggcggcttc
     2941 gtgcaggccg acccggcgag caacgcgctc atcatcaccg cgcccgagcc gctgtatcg
//
LOCUS       DASRDG010000326         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_281846, whole genome shotgun sequence.
ACCESSION   DASRDG010000326 DASRDG010000000
VERSION     DASRDG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281846"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VFA35_05430"
     CDS             <1..1032
                     /locus_tag="VFA35_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HZT55648.1"
                     /translation="EAGSRDTLQANLLTLQAWRHWQAGRVEAAAQALAQGWALAAHTR
                     YYQLLAPLRTALAELAAFALDQAIEPAFARELIRRRQLEPPRASAGGWPWPLRITTLG
                     RFAVQVDGDALVFAGKVPKKPLALLRALIALGGHTVPQHALIDALWPDDEADAAHDAF
                     NVALHRLRKLLPRGAQALVLQDGALSLNPALCWTDAHAFELLAAPAFAPAPADTAALL
                     DTLGRALALYGGHFLVDDADAPWAISTRERLRARFNQALMRRGAALGDAGAHEEALAC
                     YRQGLEVDDLNEAFYQGAMRCCLALRRPAEGLAAYQRLRRMLSIVLRVAPSAHSESLH
                     ERLRGLGIG"
     gene            1068..1280
                     /locus_tag="VFA35_05435"
     CDS             1068..1280
                     /locus_tag="VFA35_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55649.1"
                     /translation="MTAAAPPMPAPENYIVRIYRREPHHGNRIAGTVEIVGAGTERSF
                     RNLRELRQILCAPAERTGAPSEGEIR"
     gene            1277..1726
                     /locus_tag="VFA35_05440"
     CDS             1277..1726
                     /locus_tag="VFA35_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZT55650.1"
                     /translation="MTLLMTPDRTPDAPAPTELSALLRLLTEFEPLDRMVRDATRPPL
                     RWISASRGQDLVLIHVDEVLYFQSDTKYTRIVTADAEALIRMSLRALQGELDPESFWQ
                     IHRSTIVNVRAIAGARRTPRGSWVVRLKSRAETLAISESHVHRFHQM"
     gene            complement(1778..>2182)
                     /locus_tag="VFA35_05445"
     CDS             complement(1778..>2182)
                     /locus_tag="VFA35_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT55651.1"
                     /translation="TRIVAEADKAFGGPMPAGCMYAPQAFIDKNPATVQAIANAIVRA
                     DKWIQGAGPADIVKAVPESYLLGDRAVYVDAFLAAKGALSVDGTIPEAGAETARRALA
                     SIDPEIAGAKIDLAAIWTNEFVKKANAKYAKG"
BASE COUNT          294 a          815 c          766 g          307 t
ORIGIN      
        1 gaggccggct cgcgcgacac gctgcaggcc aacctgctca ccctgcaggc ctggcggcat
       61 tggcaggccg gccgcgtcga ggccgcggcg caggcactcg cccagggctg ggcgctggcg
      121 gcgcacacgc gctactacca gctgctggcg ccgctgcgca cggcgctcgc cgaactggcg
      181 gcgttcgcgc tcgaccaggc gatcgaaccc gccttcgcgc gcgaactcat ccggcgccgc
      241 cagctcgagc cgccgcgggc cagtgcgggc ggctggccct ggccgctgcg catcaccacg
      301 ctcggacgct tcgccgtaca ggtcgacggt gacgcgctgg tcttcgccgg caaggtgccg
      361 aagaagccgc tcgcgttgct gagggcgctg atcgcgctcg gcggccacac cgtgccgcag
      421 cacgcgctga tcgacgcgct ctggcccgac gacgaggccg atgccgcgca cgacgccttc
      481 aacgtcgccc tgcatcggct gcgcaagctg ctgccgcgcg gcgcgcaggc gctggtgctg
      541 caggacggcg cgctgagcct gaaccccgcg ctgtgctgga ccgacgcgca cgcgttcgag
      601 ttgctcgcgg cgcccgcctt cgcgcccgcg ccggccgaca ccgcggcgct cctcgacacg
      661 ctgggtcggg cgctcgcgct gtacggcggc cacttcctgg tcgacgacgc cgacgcgccg
      721 tgggcgatct cgacccgcga acgcctgcgg gcgcgcttca accaggcgct gatgcggcgc
      781 ggcgccgcgc tcggcgacgc gggcgcgcac gaagaagcgc tcgcgtgcta tcgacagggg
      841 ctggaggtcg acgacctcaa cgaggccttc taccagggcg cgatgcggtg ctgcctcgcg
      901 ctgcgtcggc cggccgaggg cctcgcggcc taccagcgcc tgcgccgcat gctgtcgatc
      961 gtgctgcgcg tcgcgccgtc ggcacacagc gaatcgcttc atgaacggct gcgcggattg
     1021 ggcatcggtt aggcatcggt aaggccgggt gggctacctt gtgccgcatg accgccgccg
     1081 ccccgccgat gcctgcaccc gagaactaca tcgtccgcat ctaccgccgc gagccgcacc
     1141 acggcaaccg cattgcgggc acggtggaga tcgtcggcgc cggcaccgag cgcagcttca
     1201 ggaacctgcg cgaactccgg cagatcctgt gcgcaccggc cgaacgaacc ggcgcgccct
     1261 ccgaaggaga aatccgatga cgctcttgat gacgcccgac aggacgcccg atgcgcccgc
     1321 cccgaccgag ctgtccgcgc tgctgcggct gctgaccgag ttcgaaccgc tcgaccgcat
     1381 ggtgcgcgac gccacgcgcc cgccgctgcg ctggatcagc gcgtcgcgcg ggcaggacct
     1441 cgtgttgatc cacgtcgacg aggtgctgta cttccagtcg gacaccaagt acacgcgcat
     1501 cgtcacggcc gacgccgagg ccttgatccg catgtcgctg cgcgcgctgc agggcgagct
     1561 cgacccggag agcttctggc agatccaccg ctcgacgatc gtcaacgtgc gcgcgatcgc
     1621 cggcgcccgg cgcacgccgc gcggcagctg ggtcgtgcgc ctgaagtcgc gcgccgagac
     1681 gctggcgatc agcgagagcc acgtgcaccg gttccatcag atgtaggcgt accggatcgg
     1741 gcagcgctcg gacgcggcag gcgagcgccg cccgggctca ccccttcgcg tatttcgcgt
     1801 tggccttctt cacgaactcg ttcgtccaga tcgccgccag gtcgatcttc gcgcccgcga
     1861 tctcggggtc gatgctggcc agcgcgcggc gcgcggtctc ggcgccggcc tcggggatcg
     1921 tgccgtcgac cgagagcgcg cccttcgccg ccaggaacgc atcgacatac accgcgcggt
     1981 cgccgagcag gtagctctcg ggcacggcct tgacgatgtc ggccgggccg gcgccctgga
     2041 tccacttgtc ggcgcgcacg atggcgttgg cgatcgcctg caccgtggcc gggttcttgt
     2101 cgatgaaggc ctgcggcgcg tacatgcagc cggccggcat cggcccgccg aaggccttgt
     2161 cggcctcggc cacgatgcgg gt
//
LOCUS       DASRDG010000327         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_855185, whole genome shotgun sequence.
ACCESSION   DASRDG010000327 DASRDG010000000
VERSION     DASRDG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855185"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1189)
                     /locus_tag="VFA35_05450"
     CDS             complement(143..1189)
                     /locus_tag="VFA35_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZT55652.1"
                     /translation="MTFTPLAFSRLASSRGARLKAFALAVAALAAAGAAGAADQPVIG
                     LITKTDTNPFFVKMKEGAQAAATAKGAKLLSAAGKADGDNAGQVTAIENMIAGGAKTI
                     LITPNDSKAIVPAIKKAQAQGVMFIALDSPADPMEATDALFATDNYRAGQLIGRYAKV
                     AVGAKPVKIATLDLFPGHPVGAQRHNGFMSGFGLKAADAKSNELSKSAEIVCMADSFG
                     DQAKGQTAMENCLQKNPDINVVYTINEPAAAGAYQALKAAGKDKGVIIVSVDGGCAGV
                     KNVKAGVIAATSQQYPLKMAAMGVDAGVEYAKGGKKAKGYTDTGVTLITDKPMAGVDS
                     KDTKLGTDLCWGNK"
     gene            1402..>2236
                     /locus_tag="VFA35_05455"
     CDS             1402..>2236
                     /locus_tag="VFA35_05455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HZT55653.1"
                     /translation="MILVCGEALLDVFAAGETRTGLQLDAHVGGSPFNVAIGLARLRQ
                     PVAFLSAISTGFAGERLVRALVQEGVDTRAVARLDAPTTLGLIGLDARGVPAYAFYGE
                     GCADRLLKASHLAAVPAGLSAIQLGSYATVVGSTAATLRTLVERERGRVPIAFDPNVR
                     LNVEPDLGRWREHLAWMLPRCDLLKISEEDLQLLQPRVSLEAFAAQALAQGVRLVVVT
                     RGALGASGWAAGAHASVPPVAVPVIDTVGAGDTFQAALLTWLAEHGRLSSATLGALDE
                     AA"
BASE COUNT          282 a          762 c          799 g          393 t
ORIGIN      
        1 tgaggagtgg gcagtggcgc gtcgttcgcg cagaaaccgc ggcgagcggg ccggggtggc
       61 ggcgaggagc ggagccgtac tgccgtacgg cgagcaccgc agccgtcacc ccggcccgcg
      121 caggagcttt atgcgtgaac gactacttgt tgccccagca caggtcggtg ccgagcttgg
      181 tgtccttgct gtcgacgccg gccatcggct tgtcggtgat cagcgtgacg ccggtgtcgg
      241 tgtagccctt ggccttcttg ccgcccttgg cgtactcgac gccggcgtcg acgcccatcg
      301 ccgccatctt cagcgggtat tgctgcgagg tcgccgcgat cacgccggcc ttgacgttct
      361 tgacgccggc acagccgccg tcgaccgaga cgatgatgac gcccttgtcc ttgcccgccg
      421 ccttcagcgc ctggtacgcg cccgccgcgg ccggctcgtt gatcgtgtag acgacgttga
      481 tgtcggggtt cttctggagg cagttctcca tcgccgtctg gcccttggcc tggtcgccga
      541 agctgtcggc catgcagacg atctcggcgc tcttggagag ctcgttgctc ttcgcatcgg
      601 cggctttcag cccgaagccc gacatgaagc cgttgtggcg ctgcgcgccc accgggtggc
      661 cggggaacag gtcgagcgtg gcgatcttca ccggcttggc gccgaccgcc accttcgcgt
      721 agcggccgat caactggccg gccctgtagt tgtcggtcgc aaacagcgca tcggtcgcct
      781 ccatcgggtc ggccgggctg tcgagcgcga tgaacatcac gccctgcgcc tgcgccttct
      841 tgatcgccgg cacgatcgcc ttcgagtcgt tcggcgtgat caggatggtc ttggcgccgc
      901 cggcgatcat gttctcgatc gccgtcacct gcccggcgtt gtcgccgtcg gccttgccgg
      961 ccgcgctcag cagcttcgcg cccttggcgg tcgcggcggc ctgggcgccc tccttcatct
     1021 tgacgaagaa cgggttggtg tcggtcttcg tgatcagccc gatcaccggc tggtcggcgg
     1081 cacccgcggc gcccgccgcg gccagggccg cgacagcgag ggcgaaggcc ttgaggcgcg
     1141 caccgcgcga ggaggcgagg cgcgagaagg cgagaggcgt gaaggtcatg tcgtttgtct
     1201 ccgtctggat ttgcggcacc gccgctggtt ggggcccgac acctcgtcgg gtgtgttctc
     1261 attgtcggca gggtcgatta ataaatcaac tgaatttatt aatggaaaac ccgtatggtc
     1321 gcgccccgcc gcaccgggca gagtgctgcc gcgccgcggc atgttgcccg gcgcccaacc
     1381 agatcgacaa ggagccacgg gatgatcctc gtctgtggag aagcgctgct cgacgtgttc
     1441 gcggccggcg aaacgcgcac gggcttgcag ctcgatgcac atgtgggcgg ttcgcccttc
     1501 aatgtcgcga tcggcctggc gcggctgcgc cagccggtcg cctttctgag cgcgatctcg
     1561 accggttttg ccggcgagcg cttggtgcgc gcgctggtgc aggaaggcgt cgacacgcga
     1621 gcggtcgcgc gcctggatgc acccacgacg ctggggctga tcggcctcga cgcgcgcggc
     1681 gtgccggcgt atgccttcta cggcgaaggc tgcgccgatc ggctgctgaa ggcgtcgcac
     1741 ctggcggccg tgcccgccgg gctgagcgcg atccagctcg gctcgtacgc gaccgtcgtc
     1801 ggctcgaccg ctgccacgct gcgcacgctg gtcgaacgcg agcgcggccg cgtgccgatc
     1861 gcgttcgacc cgaacgtgcg tctgaacgtc gagcccgacc tcggccgctg gcgtgagcac
     1921 ctggcctgga tgctgccgcg ctgcgacctg ctgaagatca gcgaggaaga cctgcagctg
     1981 ctgcagccgc gcgtctcgct cgaggcgttt gccgcgcagg cgctcgcgca aggggtcagg
     2041 ctggtcgtgg tcacgcgcgg cgcgctcggc gcctcgggct gggccgcggg cgcgcacgcg
     2101 agcgtgccgc cggtggccgt gccggtgatc gacacggtcg gcgccggcga caccttccag
     2161 gccgcgctgc tgacctggct cgcggagcac gggcggctga gctccgccac gctcggcgcg
     2221 ctcgacgagg ccgcgc
//
LOCUS       DASRDG010000328         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1311645, whole genome shotgun sequence.
ACCESSION   DASRDG010000328 DASRDG010000000
VERSION     DASRDG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311645"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /gene="ruvB"
                     /locus_tag="VFA35_05460"
     CDS             <1..1007
                     /gene="ruvB"
                     /locus_tag="VFA35_05460"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZT55654.1"
                     /translation="GEPAPAEGGDKALRPQTLAEFVGQAPIKANLSIFIDGARSRGEA
                     LDHVLLFGPPGLGKTTLSHIIANELGVNLRQTSGPVLEKPKDLAAILTNLERNDVLFI
                     DEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSIKLDLQPFTLIGATTRAGMLTNP
                     LRDRFGIVARLEFYTAEELARIVHRSAGLLNVPTDDEGAFEIARRSRGTPRIANRLLR
                     RVRDYADVKADGRIGRLLADKALKMLDVDAHGLDVMDRKLLEAVVHRFDGGPVGLDNV
                     AAAIGEERDTIEDVIEPYLIQQGYLQRTPRGRVATPAAYRHLGVTPPKRSGAADLFEP
                     "
     gene            complement(1012..1416)
                     /locus_tag="VFA35_05465"
     CDS             complement(1012..1416)
                     /locus_tag="VFA35_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55655.1"
                     /translation="MSIKRLTKEDIRGESGILNPVGHVVLAFKDDAVAVQAVDALHQA
                     GAANQDILSYTAAEATPRLQERVRTASAGAGFGYEITLMRRYLAFAEEGAGWLIVYAP
                     DDAAVQRITEVAKRFDALCAVRYRRLANEDLI"
     gene            complement(1487..2452)
                     /locus_tag="VFA35_05470"
     CDS             complement(1487..2452)
                     /locus_tag="VFA35_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZT55656.1"
                     /translation="MSAGINNNDTVARLSRIGLWAGLALLLAAPFIGLYPVFMMKALC
                     YAIFACAFNLLLGYTGLLSFGHAAFLGAAAYATGWLVRSAGWSPELGVLAGTAIAAAC
                     GLVIGLIAIRRQGIYFAMVTLAMAQMLYFVFLQAPFTGGEDGLQGVPRGKLFGLLPLA
                     DDKVLYYVVLAVFVAVFLFIIRIVHSPYGQVLKAIRENEPRAISLGYDVDRYKLLAFV
                     LSTAIAGLAGSIKTIVLGFASLTDAHWSLSGEVILMTLLGGMGTFAGPVIGAFTIIGL
                     QDFLADQVGSWINVIIGVIFVVCVVAFRKGFVGELLAWLRRRKGH"
     gene            complement(2452..>2877)
                     /locus_tag="VFA35_05475"
     CDS             complement(2452..>2877)
                     /locus_tag="VFA35_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZT55657.1"
                     /translation="VFSLGVCLATWFVIERTKLGSYLRAATENPALVQAFGVNVPRMI
                     TLTYGIGVGLAALAGVMAAPIYQVNPLMGADLIIVVFAVVVIGGMGSILGSILTGLAL
                     GVIEGLTKVFYPEASSIVVFVIMAIVLMIRPAGLFGKEK"
BASE COUNT          519 a         1003 c          962 g          393 t
ORIGIN      
        1 ccggcgagcc cgcgccggcg gaaggcggcg acaaggcgct gcgcccgcag accctggccg
       61 agttcgtggg tcaggcgccg atcaaggcca acctgtcgat cttcatcgac ggcgcccggt
      121 cgcgcggcga ggcgctggac cacgtgctgc tgttcggccc gccggggctg gggaagacga
      181 ccttgtcgca catcatcgcc aacgagctcg gcgtgaacct gcgccagacc tcgggcccgg
      241 tgctcgagaa gccgaaggat ctggcggcga tcctgaccaa cctcgagcgc aacgacgtgc
      301 ttttcatcga cgagatccac cgcctctcgc cggtcgtcga ggagatcctg taccccgcgc
      361 tggaggacta ccagatcgac atcatgatcg gcgaaggccc ggccgcgcgc tcgatcaagc
      421 tcgatctgca gcccttcacg ctgatcggcg cgaccacccg cgcgggcatg ctgaccaacc
      481 cgctgcgcga ccgcttcggc atcgtcgcgc gtttggagtt ctatacggcc gaagagctcg
      541 cgcgcatcgt gcaccgctcg gccgggctgc tgaacgtgcc gaccgacgac gagggcgcgt
      601 tcgagatcgc gcgccgctcg cgcggcacgc cgcgcatcgc gaaccgcctg ctgcgccgcg
      661 tgcgcgacta cgccgacgtg aaggccgacg gccgcatcgg ccggctgctg gccgacaagg
      721 cgctgaagat gctcgatgtc gacgcgcacg gcctcgacgt gatggaccgc aagctgctcg
      781 aagccgtggt gcaccgcttc gacggcgggc cggtcggtct cgacaacgtc gccgccgcga
      841 tcggcgagga gcgcgacacg atcgaggacg tgatcgagcc ctacctgatc cagcagggct
      901 acctgcagcg cacgccgcgc gggcgggtcg cgacgccggc ggcgtaccgg cacctcgggg
      961 tcacgccacc gaaacggtcg ggggcggccg atctgttcga gccatgagac gctagatcag
     1021 atcctcgttc gcgagccggc ggtaacgcac cgcgcacagc gcatcgaagc gctttgcgac
     1081 ctcggtgatg cgctgcaccg cggcgtcgtc cggcgcgtag acgatcagcc agccggcacc
     1141 ttcttcggcg aacgcgaggt agcgccgcat caaggtgatc tcgtagccga agcccgcgcc
     1201 ggcgctcgcg gtgcgcaccc gttcctgcag gcgcggcgtg gcctccgcgg cggtgtacga
     1261 caggatgtcc tggttcgccg cgccggcctg gtgcagcgcg tcgaccgcct gcaccgcgac
     1321 cgcgtcgtct ttgaacgcga gcaccacgtg cccgaccgga ttcaggatgc cggattcccc
     1381 gcggatgtct tccttggtca ggcgtttgat gctcatctcg gcgtctcctc gaatggcacg
     1441 aaggccagtc tagacaccgc gacgccggct tgccaagccc cctgcgtcaa tgccccttgc
     1501 gccgccgcag ccaggccagc agttcgccga cgaagccctt gcggaacgcg accacgcaga
     1561 cgacgaagat cacgccgatg atcacgttga tccacgagcc gacctgatcg gcgagaaagt
     1621 cctgcagccc gatgatcgtg aacgcaccga tcaccggccc ggcaaacgtg cccatgccgc
     1681 cgagcagcgt catcaggatc acctcgcccg agagcgacca gtgcgcgtcg gtcaggctcg
     1741 cgaagccgag cacaatcgtc ttgatcgaac cggcgaggcc ggcgatcgcg gtggagagca
     1801 cgaaggcgag cagcttgtag cggtcgacgt cgtagcccag cgagatcgcg cgcggctcgt
     1861 tctcgcggat cgccttcagc acctggccgt agggcgaatg cacgatgcgg atgatgaaca
     1921 ggaacaccgc gacgaacacc gcgagcacga cgtagtacag caccttgtcg tcggcgagcg
     1981 gaagcaaccc gaacagcttg ccgcgcggca cgccctgcag gccgtcctcg ccgccggtga
     2041 acggcgcctg caggaacacg aagtacagca tctgcgccat cgccagcgtg accatcgcga
     2101 agtagatgcc ctggcggcgg atcgcgatca gcccgatcac gaggccgcag gccgcggcga
     2161 tcgcggtgcc agcgagcacg cccagctccg gtgaccagcc ggcgctgcgc acgagccagc
     2221 cggtggcata ggccgccgcg ccgaggaagg cggcatggcc gaacgagagc agcccggtgt
     2281 agccgagcag caggttgaac gcgcacgcga agatcgcgta gcacagtgcc ttcatcatga
     2341 agaccgggta caggccgatg aacggcgccg cgagcagcag cgcgaggccg gcccacaggc
     2401 cgatgcggct caggcgagcg acggtatcgt tgttgttgat tcccgcgctc atcacttttc
     2461 cttcccgaac aagccggccg gccggatcat cagcacgatc gccatgatca cgaacacgac
     2521 gatgctggag gcttcggggt agaacacctt ggtcaggccc tcgatgacgc cgagcgcgag
     2581 gccggtcagg atcgagccga gaatcgagcc catgccgccg atcacgacga ccgcgaacac
     2641 cacgatgatg aggtcggcgc ccatcagcgg attgacctgg tagatcggcg ccgccatcac
     2701 gcccgcgagc gccgccaagc cgacgccgat cccgtacgtc agagtgatca tccgcgggac
     2761 gttgacaccg aacgcctgca ccagcgccgg gttctcggtg gccgcgcgca ggtaggagcc
     2821 gagcttggtg cgctcgatga cgaaccacgt ggccaggcag acgccgagcg agaacac
//
LOCUS       DASRDG010000329         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_855238, whole genome shotgun sequence.
ACCESSION   DASRDG010000329 DASRDG010000000
VERSION     DASRDG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855238"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFA35_05480"
     CDS             <1..447
                     /locus_tag="VFA35_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55658.1"
                     /translation="ADNNSSVQIEWHLKSDSSVELGGWTIDDVELGTRTTPPLPAQLQ
                     MVPEQAAANQAMVLTVLTNGPQPFVLGIGDTQGPTLIPGIPPVLVGGNLTTLSGFTNA
                     FGLTQVAFTAFPGAPATGLFWYSQVVTLDPTSAIVVSNPCITLFTP"
     gene            556..2217
                     /locus_tag="VFA35_05485"
     CDS             556..2217
                     /locus_tag="VFA35_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55659.1"
                     /translation="MRQALLLFAVSLCVRLCFWLATPDRGGPFEAAYQGDALRWQQLA
                     APGAAHSDDHEAVLPWRPPAMTWLVRALGEGTPTVKSDDPVAAPRTRALLALLGSALA
                     PLLWLLLRGPLPARTAALAGWLVALAHPLVVLGSGVHSEIPYLFVFVLSLLAWAPMCA
                     RRAPAAAVTFGALQALACLFRADHVLTTALLLALLLAARNAPWRRRNALLAALAGALA
                     LLPWHLHARAMVDAYDTANAPVLPLPGALPWDEPALAAVRAMPAFAQLAAFRVVDATV
                     RVRGGSRVTAAGVGVLDEAYDSLPDAIPPPLVAIYGPLNFFLGNSPEADGAFARKALD
                     RRPPLRGGPSRYPPGLVDGLPALALDYPPHLQALNHGYALGLREIAADPLAGAARCWR
                     KLAFGWQGAAATFGSGALPLGTAGERRAVDLTTPVGAGATAWRALLLALAALGAWRLR
                     RARALLPWAAWLLGKAMTLLLFFGYARLGALMIPALAPAWAAALEALATRLPAVWPRR
                     LGWLALAAVAAGELARVVAPPQQTVDGVPFVVGAHIRHETAIVRY"
BASE COUNT          234 a          881 c          801 g          323 t
ORIGIN      
        1 gcagacaaca actcctccgt gcagatcgag tggcacctca agagcgacag cagcgtggag
       61 ctcggcggct ggacgatcga cgacgtcgag ctcggcacgc gcacgacccc gccgctgccc
      121 gcgcagttgc agatggtgcc cgagcaggcc gcggccaacc aagcgatggt cctgacggtg
      181 ctgacgaacg gcccgcaacc gttcgtgctc ggcatcggcg acacgcaggg tccgacgctg
      241 atccccggca tcccgccggt gctcgtcggc ggcaatctga ccacgctgtc gggcttcacc
      301 aacgcgttcg gcctgaccca ggtcgccttc accgcgttcc ccggcgctcc ggcgaccggc
      361 ctgttctggt acagccaggt ggtcacgctc gaccccacga gcgcgatcgt cgtctcgaat
      421 ccgtgcatca ccctgttcac accgtgaccg ccggcgcccg cagggcgccg acgaacgcat
      481 gaactcgccg aaccggggcg caggtcgccg acctgcaccc cggtttgctg tacggccgcg
      541 gtatggtgcg cgcccgtgcg ccaggccctc ctgctgttcg cggtgtcgct gtgcgtgcgg
      601 ctctgcttct ggctcgcgac cccggaccgc ggcggcccgt tcgaggccgc ctaccagggc
      661 gacgcgctgc gctggcagca gctcgccgcg cccggcgccg cgcacagcga cgaccacgag
      721 gcggtgctgc catggcggcc gccggcgatg acctggctcg tgcgcgccct cggcgaaggc
      781 acgccaacgg tgaagtccga tgacccggtc gcggcgccgc gaacccgcgc actgctcgcg
      841 ctgctcggca gcgcgctggc gccgctcttg tggctgttgc tgcgcgggcc gctgccagcg
      901 cgcaccgcgg cgctcgcggg ctggctcgtc gcgctggcgc atccgctggt cgtgctcggc
      961 agcggcgtgc acagcgagat cccgtacctg ttcgtgttcg tgctgtcgct gctcgcgtgg
     1021 gcgccgatgt gcgcgcggcg tgcgccggcc gcggcggtga cgttcggcgc gctgcaggcg
     1081 ctggcctgcc tgttccgcgc cgaccacgtg ctcacgaccg cgctgctgct ggctttgctg
     1141 ctggccgcgc gcaacgcgcc ctggcgccgc cgcaacgcgc tgctcgccgc gctcgccggc
     1201 gcgctcgcgc tgctgccctg gcacctgcac gcgcgcgcca tggtggatgc gtacgacacc
     1261 gccaacgcgc ccgtgctgcc gctgcccggc gcactgccgt gggacgaacc ggcgctcgcg
     1321 gccgtgcggg ccatgccggc cttcgcccag ctggccgcgt tccgcgtcgt cgacgcaacc
     1381 gtgcgcgtgc gcggcggcag ccgagtcacg gcggccggcg tcggcgtgct cgacgaggcc
     1441 tacgactccc tgcccgacgc gatcccgccg ccgctggtgg cgatctacgg cccgctcaac
     1501 ttcttcctcg gcaactcgcc ggaggcggac ggcgcgttcg cgcgcaaggc actcgaccgc
     1561 cgcccgccgc tgcgcggcgg cccgtcgcgc tacccgccgg ggctggtcga cgggctgccg
     1621 gcactcgcgc tcgactaccc gccgcacctg caggcgctca accacggcta cgcgctcggc
     1681 ctgcgcgaga tcgccgccga tccgctcgcc ggcgcggcgc gctgctggcg caagctcgcg
     1741 ttcggctggc agggcgcggc ggcgacgttc ggcagcggcg cgctcccgct cggcaccgcg
     1801 ggcgaacggc gcgccgtcga cctgacgaca ccggtcggcg ccggcgccac tgcgtggcgc
     1861 gcgctgctgc tcgcgctcgc cgcgctcggc gcgtggcgcc tgcgccgcgc acgcgcgctg
     1921 ctgccctggg cggcctggct gctcggcaag gcgatgacgc tgctgctgtt cttcggctac
     1981 gcgcggctcg gcgcgctgat gatcccggcg ctggcaccgg cgtgggccgc cgcactcgag
     2041 gcactggcca cgcggctgcc ggcggtctgg ccgcgccggc tcggctggct cgcgctcgcc
     2101 gcggtcgccg ccggcgaact cgcgcgcgtc gtcgcgccgc cgcagcagac ggttgacggc
     2161 gtgccgttcg tcgtcggtgc gcacatacgt cacgagaccg cgatcgtgcg ctactgatgc
     2221 gcttgcttct gcagctgct
//
LOCUS       DASRDG010000330         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1311726, whole genome shotgun sequence.
ACCESSION   DASRDG010000330 DASRDG010000000
VERSION     DASRDG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311726"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /gene="gcvPB"
                     /locus_tag="VFA35_05490"
     CDS             <1..1557
                     /gene="gcvPB"
                     /locus_tag="VFA35_05490"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZT55660.1"
                     /translation="DIDEVDRTTANALPEGLLRATDFLTHDVFNSHHSETQLLRYLHE
                     LSSKDYALDRGMIPLGSCTMKLNATSEMIPITWPEFANVHPFAPLDQLAGYEQLRVQL
                     EDWLCQATGYSGVSLQPNAGSQGEYAGLLAIRAFHAARGQAQRTICLIPESAHGTNPA
                     SAQLAGLQVVVTKCDADGNVDLADLEARCLQHRERLAAVMITYPSTYGVFERRVKELC
                     AIVHCHGGRVYVDGANMNALIGVAAPGEFGGDVSHLNLHKTFCIPHGGGGPGVGPVCV
                     VADLVPYLPGHAASGRPGQGVGAVSAAPLGNAAVLPISWMYMRMMGAEGLQRATETAI
                     LSANYIAARLCDHYELHYSGNVAGIKGGGVAHECILDLRPLKDATGGAQGVSAEDVAK
                     RLIDYGFHAPTLSFPVPGTLMVEPTESESLAELDRFCEAMIAIRAEIAQVEQGAWPRD
                     DNPLKHAPHTAEALLAAEWPHPYTREQAAYPLAALRGKKYWSPVGRVDNVWGDRNLSC
                     SCPPLSAYED"
     gene            1629..2054
                     /locus_tag="VFA35_05495"
     CDS             1629..2054
                     /locus_tag="VFA35_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZT55661.1"
                     /translation="MTIETLAKNVFSHVKPADTAWRSGGLRDFFLYKDLGIEAATGGR
                     VIAQLVKANQAPAKGTGWHRHEAQFHIVLMLKGWAKFMYGNKDTRVEAGDCVHQRPGI
                     VHFLYDYSPDMEYLEVVGPADFNTVDMPAPAAVPDPKPW"
     gene            complement(2082..>2481)
                     /locus_tag="VFA35_05500"
     CDS             complement(2082..>2481)
                     /locus_tag="VFA35_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HZT55662.1"
                     /translation="PADRKARAHCRAICGEMHSGFSALRSALPMNLKARYDNFRVWAR
                     AQADIERISVIWRICINTYGGPYLFGSQPCMADAMFAPVVTRFLTYDVKLNKPCILYC
                     QTIMAMPEMKEWVAAAKLEPDDIEELDMDF"
BASE COUNT          415 a          891 c          810 g          365 t
ORIGIN      
        1 gacatcgacg aggtcgaccg caccaccgcc aacgcgctgc ccgagggact gctgcgtgcc
       61 accgacttcc tgacgcacga cgtcttcaac agccaccaca gcgagaccca gctgctgcgc
      121 tatctccacg agctgtccag caaggactac gccctggacc gcggcatgat cccgctcggc
      181 tcctgcacga tgaagctgaa cgcgaccagc gagatgatcc cgatcacctg gcccgagttc
      241 gcgaacgtgc acccgttcgc gccgctcgat cagctcgccg gctacgagca gctgcgcgtg
      301 cagctcgaag actggctgtg ccaggccacc ggctacagcg gcgtgagcct gcagcccaac
      361 gccggctcgc agggcgaata cgccggcctg ctcgcgatcc gcgcgttcca tgcggcgcgc
      421 gggcaggcgc agcgcacgat ctgcctgatc cccgagtcgg cgcacggcac caacccggcg
      481 agcgcccagc tggcgggctt gcaggtcgtc gtcaccaagt gcgacgccga cggcaacgtc
      541 gacctcgccg acctcgaggc aaggtgcctg cagcaccgcg agcgcctggc cgcggtgatg
      601 atcacctacc cctcgaccta cggcgtgttc gagcgccggg tcaaggagct ctgcgccatc
      661 gtccattgcc acggcggccg ggtctacgtc gacggcgcga acatgaacgc gctgatcggc
      721 gtggccgccc cgggcgagtt cggcggcgac gtcagccacc tcaacctgca caagaccttc
      781 tgcattccgc acggcggcgg tggcccgggc gtcggcccgg tctgcgtcgt cgccgacctg
      841 gtgccctacc tgcccggcca cgccgccagc ggtcggccgg gccagggtgt cggtgcggtc
      901 tcggcggcgc cgctcggcaa tgccgcggtc ctgccgatca gctggatgta catgcggatg
      961 atgggcgccg agggcctgca gcgcgccacc gagaccgcca tcctctcggc caactacatc
     1021 gccgcccgcc tctgtgacca ctacgagctg cactacagcg gcaacgtcgc cggcatcaag
     1081 gggggcggcg tcgcccacga gtgcatcctc gacctgcggc ctttgaaaga tgccaccggc
     1141 ggcgcccagg gggtaagcgc agaagacgtc gccaagcggc tgatcgacta cggctttcat
     1201 gcgccgaccc tcagcttccc ggtgcccggc acgctgatgg tcgagccgac ggagagcgaa
     1261 tcgctggccg agctcgaccg cttctgcgag gcgatgatcg ccatccgggc cgagatcgcg
     1321 caggtcgagc aaggcgcctg gccgcgcgac gacaacccgc tcaagcacgc gccgcacacc
     1381 gcggaggcgc tgctcgccgc cgagtggccg catccgtaca cgcgcgagca ggcggcctat
     1441 ccgctcgcgg cgctgcgcgg caagaagtac tggtcgcccg ttggccgggt cgacaacgtc
     1501 tggggcgacc gcaacctctc ctgctcctgc ccgccgcttt ccgcctacga ggattgacgc
     1561 ggcgcagcaa gactggaacg gcttatgcgt actccgccgc atcgcaactg cggcagggga
     1621 ggtcgcccat gacgatcgaa acgctggcga agaacgtctt ttcccacgtc aagcccgcgg
     1681 acaccgcctg gcgcagcggc ggcctgcgcg acttcttcct ctacaaggac cttggcatcg
     1741 aggcggcgac cggcgggcgc gtcatcgccc agctcgtcaa ggccaaccag gcaccggcga
     1801 agggcacggg ctggcaccgg cacgaggcgc agttccacat cgtgctcatg ctgaagggct
     1861 gggccaagtt catgtacggc aacaaggaca cgcgggtcga ggccggcgac tgcgtccacc
     1921 agcgcccggg aatcgtgcac ttcctctacg actactcgcc cgacatggag tacctcgagg
     1981 tggtcggtcc ggccgacttc aacaccgtcg acatgccggc gccggcggcg gtgccggatc
     2041 cgaagccctg gtaaggctcg gccgggccgc cttcgcccgg cctagaagtc catgtcgagc
     2101 tcttcgatgt cgtcgggctc gagcttcgca gcggcaaccc actccttcat ctcgggcatc
     2161 gccatgatgg tctggcagta caggatgcag ggcttgttca gcttcacgtc ataggtcaga
     2221 aagcgcgtga cgaccggcgc gaacatcgcg tcggccatgc agggctggct gccgaacagg
     2281 tagggaccgc cataggtgtt gatgcagatc cgccagatca cgctgatgcg ttcgatgtcg
     2341 gcctgcgcgc gcgcccacac ccgaaagttg tcgtacctgg ccttgaggtt catcggcagc
     2401 gccgagcgca atgccgaaaa gcccgaatgc atttcgccgc agatggcgcg gcaatgcgcc
     2461 cgcgccttgc ggtcggccgg c
//
LOCUS       DASRDG010000331         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1203231, whole genome shotgun sequence.
ACCESSION   DASRDG010000331 DASRDG010000000
VERSION     DASRDG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203231"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFA35_05505"
     CDS             complement(<1..585)
                     /locus_tag="VFA35_05505"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HZT55663.1"
                     /translation="MLLRLLLCAGLALLLGAPASAAPDALPAEVGAALDRANVPRDAV
                     VAMVQEVASARPRLAWQAERPANPASLMKLLTTQAALELLGPAWSWSTPVWLQGPVRD
                     GVLDGDLVIKGSGDPKLVLERLWLLLRRVQQLGVREIRGDIVLDRSDFKVPEQSPADF
                     DGEALRPYNVRADALLLNYKAVVFTFTPDAARSVA"
     gene            642..1364
                     /gene="tsaB"
                     /locus_tag="VFA35_05510"
     CDS             642..1364
                     /gene="tsaB"
                     /locus_tag="VFA35_05510"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZT55664.1"
                     /translation="MPDALLAFDTATEHMSIALAVRGQVFAHEAAGGAQASATLIPAI
                     LALLAQAGITLRELDAIAFGRGPGAFTGLRTACSVAQGLAFGAAKPVLPIDTLLAVAE
                     DARGGDRRAQRVGVAMDARMNEVYAARYAFGDGRWSVLDAPLLTTPEALSARWAAQPP
                     DLVAGSALAAFGDRLATRGARRAPGALPRARAMLPLARALWAAGGAVDAALALPLYVR
                     DKVAQTTVERDAARLAKLEVGR"
     gene            1403..1861
                     /gene="rimI"
                     /locus_tag="VFA35_05515"
     CDS             1403..1861
                     /gene="rimI"
                     /locus_tag="VFA35_05515"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZT55665.1"
                     /translation="MTAAQVDAVMAIEAAAYASPWSRGNFIDSLAAGYPAQLLRGVRG
                     ELLGYFVAMGGVDEMHLLNITVAPAVQGCGHARTLFDALIALCHAQRARELWLEVRAG
                     NARARAVYAHLGFAQVGLRKDYYPAPFGRREDAIVMSLKFDAAGANDALG"
     gene            1848..2609
                     /locus_tag="VFA35_05520"
     CDS             1848..2609
                     /locus_tag="VFA35_05520"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZT55666.1"
                     /translation="MRWDERQVAMLREMEIRLWFPQPDPVPASVSTPLPLAERGVGAA
                     GASHDTVVVGRPPEQAASSLDWPALREAVAACTACKLCEGRRQTVFGVGNERAHWMIV
                     GEAPGEQEDRQGEPFVGKSGQLLDNMLRAIGLTRAAAEPARQVYIANTIKCRPPANRN
                     PEPEELARCEPFLVRQVQLVQPKIIVAMGRFAVHSLLRSTEPIGRLRGRVHAYQGVPL
                     IVTYHPAYLLRNPQDKAKAWDDLCLALETAGAQSG"
BASE COUNT          367 a         1018 c         1043 g          357 t
ORIGIN      
        1 cgcgacgctg cgcgccgcat cgggcgtgaa cgtgaagacc acggccttgt agttcagcag
       61 cagcgcgtcg gcgcgcacgt tgtacggccg cagcgcctcg ccgtcgaaat cggccgggct
      121 ttgctcgggc accttgaagt cgctgcggtc gagcacgatg tcgccgcgga tctcgcgcac
      181 gccgagctgc tgcacgcggc gcaacagcag ccacaggcgt tcgagcacga gcttggggtc
      241 gccgctgccc ttgatcacga ggtcgccgtc gagcacgccg tcgcgcaccg ggccctgcag
      301 ccacaccggc gtcgaccagc tccaggccgg gccgagcagt tcgagcgccg cctgggtcgt
      361 cagcagcttc atcagcgagg cggggttcgc cggccgctcg gcctgccagg ccagacgcgg
      421 gcgcgcgctg gccacctcct gcaccatcgc gacgaccgca tcgcgcggca cgttcgcgcg
      481 gtcgagtgcg gcgccgacct cggccggcag cgcgtcgggg gcggccgacg cgggcgcgcc
      541 gagcagcagc gccaggccgg cgcacaacag aaggcgcagg agcatcgggc gatgatcgca
      601 cgcgggcgcg gcggccaaag cgcggcggct aaactcgttg catgcccgac gcgctgctcg
      661 ccttcgacac cgccaccgaa cacatgagca tcgcgctcgc ggtgcgcggc caggtgttcg
      721 cgcacgaggc ggcgggcggc gcgcaggcct cggccacgct gatcccggcg atcctcgcgc
      781 tgctcgcgca ggccgggatc acgctgcgcg agctcgacgc gatcgcgttc ggtcgcgggc
      841 cgggcgcgtt caccgggctg cgcacggcgt gctcggtcgc gcagggcctg gcgttcggcg
      901 ccgccaagcc ggtgctgccg atcgacacct tgctcgcggt cgccgaagac gcgcgtggcg
      961 gcgaccgccg cgcgcagcgc gtgggggtcg cgatggacgc gcgcatgaac gaggtctacg
     1021 ccgcgcgcta tgcgttcggc gacggtcgtt ggagcgtgct cgacgcgccg ctgctgacga
     1081 cgcccgaggc gctcagcgca cgctgggcgg cgcagccgcc tgacctggtc gccggcagcg
     1141 cgctcgccgc gttcggcgat cggctggcga cgcgcggcgc acgccgcgcc cccggcgcgc
     1201 tgccgcgcgc gcgcgcgatg ctgccgctgg cgcgcgcgct gtgggccgcc ggcggcgccg
     1261 tcgacgcggc gctcgcgctg ccgctctacg tgcgcgacaa ggtggcgcag accacggtcg
     1321 agcgcgacgc agcgcggctc gcgaagctgg aggtagggcg gtgagtgcgc agtggaaaga
     1381 caccgcgccg cttctgctgc cgatgaccgc cgcgcaggtc gacgccgtga tggcgatcga
     1441 ggccgctgcc tacgcgtcgc cctggagccg cggcaatttc atcgactcgc tcgccgccgg
     1501 ctacccggcg cagctgctgc gcggcgtgcg cggcgaactg ctcggctact tcgtcgcgat
     1561 gggcggcgtc gacgagatgc acctgctgaa catcacggtg gcgcccgcgg tgcagggctg
     1621 cggccatgcg cgcacgctgt tcgatgcgct gatcgcgctg tgccacgcgc agcgcgcgcg
     1681 cgagctctgg ctcgaggtgc gcgccggcaa cgcgcgcgcg cgcgccgtgt acgcgcacct
     1741 gggcttcgcg caggtgggcc tgcgcaagga ctactacccg gcgccgttcg ggcgccgcga
     1801 ggacgcgatc gtgatgagcc tgaagttcga cgccgcggga gcgaacgatg cgctgggatg
     1861 agcgccaggt cgcgatgctg cgcgagatgg agatcaggtt gtggttccca caacctgacc
     1921 ccgttccggc gtccgtgagc acccccctgc ccctcgccga gcggggggtg ggcgcggctg
     1981 gcgcctcgca tgacacggtc gtcgtggggc ggccgccgga acaggctgcg tcgtcgctgg
     2041 actggcctgc gctgcgcgag gccgttgctg cctgcaccgc ctgcaagctg tgcgagggcc
     2101 ggcgccagac cgtgttcggg gtcggcaacg agcgggcgca ctggatgatc gtcggcgaag
     2161 cgccgggcga gcaggaagac cggcagggcg agcccttcgt cggcaagtcg ggccagttgc
     2221 tcgacaacat gctgcgcgcg atcgggctga cgcgcgccgc ggccgagccg gcgcggcagg
     2281 tctacatcgc gaacacgatc aagtgccgcc ccccggcgaa ccgcaacccc gagccggaag
     2341 agctcgcgcg ctgcgagccg ttcctggtgc gccaggtgca gctggtgcag ccgaagatca
     2401 tcgtcgcgat gggccgcttc gcggtgcaca gcctgctgcg cagcaccgag ccgatcggcc
     2461 gcctgcgcgg tcgcgtccac gcgtaccagg gcgtgccgct gatcgtgacc taccacccgg
     2521 cctacctgct gcgcaacccg caagacaagg ccaaggcctg ggacgacctg tgcctggcgc
     2581 tcgaaaccgc aggtgcgcaa tcgggctgaa cccgccggtt gaacgcgccg gcggcgccgc
     2641 ggcgccccca agactatgat cctcagcgat gcccggtgcg ccaccccgcc ggcgtcccgc
     2701 gtgatggccg ttgacctgcc cgtgcaaccc gacgacgcgc gccccgagca cgcgttcgcc
     2761 gcgcgttcgc ccgaggccca ggcgg
//
LOCUS       DASRDG010000332         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1433882, whole genome shotgun sequence.
ACCESSION   DASRDG010000332 DASRDG010000000
VERSION     DASRDG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433882"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VFA35_05525"
     CDS             <1..928
                     /locus_tag="VFA35_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZT55667.1"
                     /translation="APYQTTVASAQATLARAQANLAQAAAQAERYKPLLEANAISKQD
                     YVNAVAAQKQAEADVAAAKASVQSGQINLGYAAVTAPISGRIGRALVTEGALVGQGEA
                     TPLAMVQQINPMYVNFTQSTTDVLQLRRAIASGKFKRAGGDSASVKIVLEDGSTYPQP
                     GRLLFSDLSVDPTSGQITLRAEVPNPTGLLLPGMYVRVRLEQAETPTGIVVPQQAVTR
                     GTIGDSVMVVGADGKVAPRPVKVGAAAEGGSWVILEGLQTGELVMVDGFQKLRGNAPV
                     KPVPWQPPGSAPAAGSAPAGASAPARVASAAR"
     gene            938..>2438
                     /locus_tag="VFA35_05530"
     CDS             938..>2438
                     /locus_tag="VFA35_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT55668.1"
                     /translation="MAQFFIDRPIFAWVIALFILVFGGVAIKQLPVAQFPTVAPPSIV
                     LTAVYPGASAQTLEDSVISVIEQEMNGSPGLIYFESVSQANGVGTINLTFATSTSVDL
                     AQVDVQNRLSRAAPRLPAAVIQQGVRVDKARSNFLLFTILSSDNPAFDPIALGDYASR
                     TVLPEIQRIPGVGQAQLFGTERSMRIWIDPAKLVGFNLSTNDVVAAIRAQNAQVPSGT
                     IGDLPIVAGTPISATVVVAGQLTSVEEFGQIVLRANIDGSTVKLRDVARIELGGQSYG
                     TSARLNGKPSTGIGVQLSPTGNALATAEAIKTRMTELSRYFPPGVKYDIPYDSSRFVK
                     ISISRVVETLVEAVILVFLVMYLFLQNLRYTLIPTVVVPVALFGSFGVMLVFGFSINV
                     LTMFAMVLAVGILVDDAIVVIENVERIMHEEGLSPRDATKKAMGQITAALIGITTTLV
                     SVFLPMAFFAGSVGNIYRQFSLVMVSAMLFSVFLALSLTPALCATFLKPI"
BASE COUNT          368 a          875 c          807 g          388 t
ORIGIN      
        1 cgctccctac cagacgacgg tcgcaagcgc ccaggcgacg ctcgcccggg cgcaggcgaa
       61 cctggcgcag gcggcggcgc aggccgagcg ctacaagccg ctgctcgagg ccaacgcgat
      121 cagcaagcag gactacgtca atgcggtcgc ggcgcagaag caggccgagg ccgacgtcgc
      181 cgccgccaag gcgagcgtgc agagcggcca gatcaatctc ggctatgccg cggtgaccgc
      241 accgatctcg ggccgcatcg gccgcgccct ggtgaccgag ggcgcgctcg tcggccaggg
      301 cgaggcgacg ccgctcgcca tggtgcagca gatcaatccg atgtacgtga acttcaccca
      361 gtcgacgacc gatgtcctgc agctgcgccg cgccatcgcc agcggcaagt tcaagcgcgc
      421 cggcggcgac tcggccagcg tgaagatcgt gctcgaggac ggcagcacct acccgcaacc
      481 cggccgactg ctcttctccg acctctccgt cgacccgacc tcgggccaga tcacgctgcg
      541 cgccgaggtg ccgaacccga ccgggctgct cctgcccggc atgtacgtgc gggtgcggct
      601 tgagcaggcc gagacgccga ccggcatcgt cgtgccgcag caggcggtga cgcgcggcac
      661 catcggcgac agcgtcatgg tggtcggtgc cgacggcaag gtggcgccac ggccggtcaa
      721 ggtcggcgcg gccgcggagg gcggcagctg ggtgatcctc gaaggcctgc agaccggcga
      781 gctggtcatg gtcgacggct tccagaagct gcgcggcaat gccccggtga agccggtgcc
      841 gtggcagccg cccggttcgg cgccggccgc aggcagcgca ccggccggtg cgtcggcgcc
      901 ggcgcgcgtc gcttccgccg cccgctgaga ccgcgccatg gcgcagttct tcatcgaccg
      961 gccgatcttt gcgtgggtga tcgccctctt catcctcgtc ttcggcgggg tggcgatcaa
     1021 gcagctgccg gtggcccagt tcccgaccgt cgcgccgccg tcgatcgtcc tcacggccgt
     1081 ctatccaggc gcctcggcgc agaccctcga agacagcgtg atcagcgtca tcgagcagga
     1141 gatgaacggc tcgccgggcc tgatctattt cgagtccgtg agccaggcga acggcgtcgg
     1201 cacgatcaac ctcacctttg cgaccagcac cagcgtcgac ctggcccagg tcgacgtgca
     1261 aaaccgtctc tcgcgtgccg cgccgcggct gccggccgcg gtgatccagc agggcgtgcg
     1321 cgtcgacaag gcgcgatcga acttcctcct gttcaccatc ctttcctcgg acaacccggc
     1381 gttcgatccg atcgccctcg gcgactacgc gtcgcgcacc gtgctgcccg agatccagcg
     1441 cattcccggc gtcggtcagg cgcagctgtt cggcaccgag cgctcgatgc ggatctggat
     1501 cgacccggcc aagctggtcg gcttcaacct ttccaccaat gacgtcgtcg ccgcgatccg
     1561 ggcgcagaac gcgcaggtcc cctcgggcac gatcggcgac ctgccgatcg ttgccggcac
     1621 gccgatcagc gccaccgtcg tcgtcgccgg gcagctgacc tcggtcgagg agttcggcca
     1681 gatcgtgctg cgcgccaaca tcgacggctc gaccgtcaag ctgcgcgacg tcgcccggat
     1741 cgagctcggc ggccagagct acggcacctc ggcgcggctg aacggcaagc cctccaccgg
     1801 catcggcgtg cagctctcgc ccaccggcaa cgccctggcc accgccgagg cgatcaagac
     1861 gcgcatgacc gagctgtcgc gctacttccc gcccggggtc aagtacgaca tcccgtacga
     1921 cagctcgcgc ttcgtcaaga tctcgatcag ccgggtggtg gagacgctgg tcgaggcggt
     1981 gatcctcgtc ttcctggtga tgtacctgtt cctgcagaac ctgcgctaca cgctgatccc
     2041 gacggtcgtc gtgccggtcg cgctgttcgg ctcgttcggc gtgatgctcg tcttcggctt
     2101 ctcgatcaac gtgctgacga tgttcgcgat ggtgctcgcg gtcggcatcc tggtggacga
     2161 cgcgatcgtc gtcatcgaga acgtcgagcg gatcatgcac gaggaagggc tctcgccgcg
     2221 cgacgcgacc aagaaggcga tgggccagat caccgcggcg ctgatcggca tcaccaccac
     2281 gctggtctcg gtgttcctgc cgatggcctt cttcgccggc tcggtgggca acatctatcg
     2341 ccagttctcg ctggtgatgg tgagcgcgat gctgttctcg gtgttcctcg cgctctcgct
     2401 cacgcccgcc ctgtgcgcca ccttcctcaa gccgatcg
//
LOCUS       DASRDG010000333         3182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1656836, whole genome shotgun sequence.
ACCESSION   DASRDG010000333 DASRDG010000000
VERSION     DASRDG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656836"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFA35_05535"
     CDS             complement(<1..929)
                     /locus_tag="VFA35_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZT55669.1"
                     /translation="MKHPEASFVIKYFDLIDEFIKVRCCSDDLLAELHSAAVTNKASY
                     RQRVVRACVPSFETEILAAVGKMEEDYDVEIVEELLYQICIDVNPSLEIHQVSIPSPA
                     AAQPKAENDEIARPGTEAHRQTMYRRVQNLERLLRSQIIGQEEAIASLVRAVKKTAVG
                     LKRPTAPIGTFLLVGRTGTGKTELAKAVARNLFEDPAAMIRIDCSEYALPHEYAKLIG
                     SPPGYIGHNEGGMLTEAVKKRPNCVVLFDEIEKAHYKVHNLLLQLLDEGIITDSKGQT
                     VPFHQALILMTSNLGIEKIDSIRKRMGFSAAHKR"
     gene            1043..2104
                     /locus_tag="VFA35_05540"
     CDS             1043..2104
                     /locus_tag="VFA35_05540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZT55670.1"
                     /translation="MKSETQLLALLVHRRQIDEASARAAMASGAIRDWLVGHGVCTAE
                     QWAAWQRTDGGTRPALTRYELGPLLGEGGTARVFSAVDRTDGRKVCLKVLRAELATDP
                     AQGESFVREAKLLIELSHPHLVKGYRVAREGATIFFAMEELPGRSLQEELAARGRFDE
                     TTALRIVLQVAAALDALHARGLVHRDVKPGNVLWSEPRGAVLIDLGFAVPRAKSAAGG
                     GDTTAGTVHYIAPEQARGSGELDVRADIYALGATLYQLVTGSLPFEGHSSEEVLRKQV
                     LESLSGERIRDLQLSPQVHYFIEKMMAKEREIRFQDPQQLQQEIAAFLQAQESQRELE
                     QKAQQGRPRLGHRRPRPER"
     gene            complement(2187..>3182)
                     /locus_tag="VFA35_05545"
     CDS             complement(2187..>3182)
                     /locus_tag="VFA35_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55671.1"
                     /translation="GANFYLCGRVLAKGEIEAAREDNMLSRRVTPSWNGTQWVFGQPG
                     SAITGTPLNQWTGATLRSGANTPADGRVWIGAKVTQIAGGLWHYEYAFHNRDNSRGIA
                     GFHIAKCPTAQITGVGFRDLDQSVFDDWTISVGSSSIAVLASANNALEWNTIYNVWFD
                     SDAAPAGAVVTLDEARIGAGALTFNVLDVPSPVRLANETLGPGCGSPPPQLYATGSPP
                     LPTIPNATYGLQVQAHPATAVLVAAAPLGANLPHSGGCTQYFDGPTAVTVVFAVTDAQ
                     GLLSVPIAVPNVPAFEGTDLFVQAVEAIAGGPVLGQFAASNGLRVRVGSSRTGCP"
BASE COUNT          461 a         1072 c         1162 g          487 t
ORIGIN      
        1 cgcttgtgcg ccgccgagaa gcccatgcgc ttgcggatcg agtcgatctt ctcgatgccg
       61 aggttgctgg tcatcaggat cagcgcctgg tggaacggca ccgtctgccc cttgctgtcc
      121 gtgatgatgc cctcgtcgag caactggagc agcaggttgt gcaccttgta gtgggccttc
      181 tcgatctcgt cgaacagcac gacgcagttg ggccgcttct tcaccgcctc ggtgagcatg
      241 ccgccctcgt tgtgcccgat gtagccgggc ggcgagccga tcagcttcgc gtactcgtgc
      301 ggcagcgcgt actccgagca gtcgatgcgg atcatcgccg ccggatcctc gaacaggttc
      361 cgcgcgaccg ccttcgcgag ctcggtcttg cccgtgccgg tgcggccgac gagcaggaag
      421 gtgccgatcg gagccgtggg gcgcttgagc ccgaccgcgg tcttcttgac cgcgcgcacg
      481 aggctcgcga tcgcctcctc ctggccgatg atctggctgc gcagcagccg ctcgaggttc
      541 tgcacgcggc ggtacatcgt ctgccggtgc gcctcggtgc cgggccgcgc gatctcgtcg
      601 ttctcggcct tgggctgcgc ggcggccggg gacgggatgc tgacctggtg gatctcgagc
      661 gacgggttga cgtcgatgca gatctggtag agcagctctt ccacgatctc gacgtcgtag
      721 tcctcttcca tcttgccgac cgccgcgagg atctcggtct cgaagctcgg cacgcaggcg
      781 cgcacgacgc gctggcggta cgacgccttg ttggtgacgg cggccgagtg cagctccgcg
      841 agcaggtcgt cggagcagca gcggaccttg atgaactcgt cgatcaaatc gaagtacttg
      901 atgacgaagc ttgcctctgg atgcttcacg ggacgactcc gagcaacgtc atgctgcctc
      961 catcggaagc gaactgcgcg cgacttgacg caccgggtgc ggcgcccgct agtcgttgtc
     1021 gacgcacgga ctggagcgac gattgaagtc cgagacgcag ttgctcgcgc tgctcgtgca
     1081 caggcgccag atcgacgagg cgtcagcgcg cgcggcgatg gcatcgggcg cgatccgcga
     1141 ctggctggtg ggccacggcg tgtgcacggc ggagcagtgg gccgcatggc aacgcacgga
     1201 cggcggcacg cggccggcgc tgacccgcta cgaactcggg ccactgctcg gcgagggcgg
     1261 caccgcgcgc gtgttctccg ccgtcgatcg caccgacggc aggaaggtct gcctcaaggt
     1321 gctgcgcgcc gagctggcga cggatccggc gcagggcgag tcgttcgtgc gcgaggcgaa
     1381 actgctgatc gagctgtcgc acccgcacct ggtcaagggc taccgcgtcg cgcgcgaagg
     1441 ggcgacgatc ttcttcgcga tggaggagct gcccggcagg agcctgcagg aggagctcgc
     1501 ggcgcgcggc cgcttcgacg agacgaccgc gctgcggatc gtgctgcagg tcgcagccgc
     1561 cctcgacgcg ctgcacgcgc gcgggctcgt gcaccgcgac gtcaagcccg gcaacgtgct
     1621 gtggtccgaa ccgcgcggcg cggtgctgat cgacctcggc ttcgcggtgc cgcgcgcgaa
     1681 gtcggcggcc ggcggcggcg acacgaccgc gggcaccgtg cactacatcg cgcccgagca
     1741 ggcgcgcggc agcggcgagc tcgatgtgcg cgccgacatc tacgcgctcg gcgcgacgct
     1801 ctaccagctc gtcaccggct cgctgccgtt cgaggggcac agcagcgagg aggtgctgcg
     1861 caagcaggtg ctcgagtcgc tgtccggcga gcgcatccgg gacctgcagc tgtcgccgca
     1921 ggtgcactac ttcatcgaga agatgatggc gaaggagcgc gagatccgct tccaggaccc
     1981 gcagcagctg cagcaggaga tcgccgcgtt cctgcaggcg caggagagcc agcgcgagct
     2041 ggagcagaag gcgcagcagg gccggccgcg gctcggccat cgccggccca gacccgagcg
     2101 atgaggaccg gagcgatgag gacccgagcg atgagggccg gagcgatgac ggccggccgg
     2161 ctgcgcgaat cgccgcggcg cgcgattcag ggacagccgg tgcggctgct gccgacgcgc
     2221 acgcgcaggc cgttgctggc cgcgaactgg ccgaggaccg ggccgcccgc gatcgcctcc
     2281 accgcctgca cgaagagatc ggtgccttcg aacgccggca cgttgggcac ggcgatcggc
     2341 accgacaaca ggccctgcgc atcggtcacc gcgaacacca cggtgacggc agtggggccg
     2401 tcgaagtact gcgtgcagcc gccgctgtgc ggcaggttgg cgccgagcgg cgccgcggcc
     2461 accagcacgg cggtcgccgg gtgtgcttgg acctgcaggc cgtaggtcgc gttcggaatc
     2521 gtcggcagcg gcggcgagcc ggtcgcgtag agctgcggcg gggggctgcc gcagccgggg
     2581 ccgagcgtct cgttggcgag ccgcaccggc gaaggcacgt ccagcacgtt gaacgtgagc
     2641 gcgcccgctc cgatgcgcgc ctcgtcgagc gtcacgacgg cgcccgcggg cgccgcgtcg
     2701 ctgtcgaacc acacgttgta gatcgtgttc cactcgagcg cgttgttggc gctggcgagc
     2761 accgcgatcg agctgctgcc gacgctgatc gtccagtcgt cgaacaccga ctggtcgagg
     2821 tcgcggaagc cgacgccggt gatctgcgcg gtggggcact tcgcgatgtg gaagccggcg
     2881 ataccgcggc tgttgtcgcg gttgtggaac gcgtactcgt agtgccagag tccgcccgcg
     2941 atctgcgtga ccttggcgcc gatccagacg cggccgtcgg ccggcgtgtt ggcgcccgag
     3001 cgcagcgtgg cgcccgtcca ctggttcagc ggcgtgccgg tgatcgcgct gccgggctgg
     3061 ccgaagaccc actgcgtccc gttccagctc ggtgtcacgc ggcgcgacag catgttgtcc
     3121 tcgcgcgccg cctcgatctc gcccttcgcc agcacgcggc cgcacaggta gaagttcgcc
     3181 cc
//
LOCUS       DASRDG010000334         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_164872, whole genome shotgun sequence.
ACCESSION   DASRDG010000334 DASRDG010000000
VERSION     DASRDG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164872"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1428)
                     /gene="rsmB"
                     /locus_tag="VFA35_05550"
     CDS             complement(97..1428)
                     /gene="rsmB"
                     /locus_tag="VFA35_05550"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZT55672.1"
                     /translation="MNSAPAQPASLPLAKLLNHTADAVQAVRAGESLNTALARCPAAA
                     RPGTQALGFHALRWLGAAQALRAQLAPKAPPPRVDALLLSALALLWPPGGVPYAEHTL
                     VDQAVDAGRQRSPRGAAFVNAVLRRFLRERDALVAAALREPLARFNHPAWWIERLKAD
                     WPDRWQQILDADNQRPPMTLRVNARRGDAAAYVARLAEAGMQAEAIGAQAVRLAQPCP
                     VNQLPGFAAGDVSVQDAAAQRAAPLLIGPGLPAGARVLDACAAPGGKTAHLLELADLD
                     VLALDSDPARLARVDETLARLDLTARTVAADAGTPAAWWDGRPFDAILLDAPCSAAGI
                     VRRHPDVRWLRRAGDIPVLAATQARLLDALWPLLAPGGRLLYCTCSVFKTEGQDQIDA
                     FLQRQPGAALAASPASPGHLLPLPDNAHERPVPDISAAADGFFYALIEKTT"
     gene            1520..2251
                     /gene="folE"
                     /locus_tag="VFA35_05555"
     CDS             1520..2251
                     /gene="folE"
                     /locus_tag="VFA35_05555"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HZT55673.1"
                     /translation="MRREQQPTPIFRSAEDLARLPASERIRYRLVGADCRFHANDNIS
                     AFVEDGEIDELRAEVEAKMQEVLHALVIDTDSDHNTNETAKRVAKMFLTEVFKGRYVP
                     IPPVTEFPNAERLNELMIVGPILVRSACSHHLCPIMGKVWIGIMPNEHSNLIGLSKYS
                     RICDWIMSRPQIQEEAVTMLADELDRRVKPDGLAVVMEADHFCMQWRGVKDPQSMMTN
                     SLMRGAFLKDAALRREFLSLLSRKT"
     gene            complement(2228..2554)
                     /locus_tag="VFA35_05560"
     CDS             complement(2228..2554)
                     /locus_tag="VFA35_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZT55674.1"
                     /translation="MTAGWACLTLHGATPWLAVSVQPNAKRTAADGLHDGALRVRLAA
                     PPVDGKANQVLIDWLAGELGCPKRAITLVRGETSRRKWLAIDAPLERAEAWLAATLGS
                     GLAAQQ"
BASE COUNT          387 a          971 c          990 g          358 t
ORIGIN      
        1 gccagcagca cggccgcgcg ccgacccggg cgccgcgggg cgccgggctg ggtgtcgccg
       61 tctcgtgttg ccgcgcagtg catcgggccg tgaacctcaa gtcgtcttct cgatcagggc
      121 gtagaaaaat ccgtccgcgg ccgcgctgat gtcgggcact gggcgctcgt gtgcattgtc
      181 gggcagcggc agcagatggc cgggcgatgc cgggctggcg gccagggcgg cgccgggttg
      241 gcgttgcaaa aaagcgtcga tctggtcctg gccctcggtc ttgaagaccg agcaggtgca
      301 atacagcagc cggccgccgg gcgcgagcag cggccacaag gcgtcgagca ggcgcgcttg
      361 tgtcgccgcc agcaccggga tgtcgccggc gcggcgcaac cagcgcacgt cggggtggcg
      421 gcgcacgatg ccggcggcgc tgcacggggc gtcgagcagg atcgcgtcga agggccggcc
      481 gtcccaccaa gcggcgggcg tgccggcgtc agcggcgacg gtccgcgccg tcaggtccag
      541 gcgcgccagc gtctcgtcga cgcgggccag gcgcgccggg tcgctgtcga gcgcgagcac
      601 gtcgaggtcg gcgagttcga gcaggtgcgc ggtcttgccg ccgggcgcgg cgcaggcgtc
      661 gagcacacgc gcgcccgccg gcaggcccgg cccgatcagc agcggcgcgg cgcgttgcgc
      721 ggcggcgtcc tgcaccgaga catcgccggc ggcgaagccc ggcagttggt tcaccgggca
      781 gggctgggcc agccgcacgg cctgcgcgcc gatcgcctcg gcctgcatac cggcctcggc
      841 cagccgcgcg acgtaggcgg ccgcgtcgcc gcggcgcgcg ttcacgcgca aggtcatcgg
      901 cgggcgctgg ttgtcggcgt cgaggatctg ctgccagcga tccggccagt cggccttcag
      961 ccgctcgatc caccacgccg gatggttgaa gcgcgcgagc ggctcgcgca gcgccgccgc
     1021 caccagcgcg tcgcgctcgc gcagaaagcg ccgcagcacc gcgttgacga aagccgcgcc
     1081 gcgcgggctg cgctggcgcc ccgcgtcgac cgcctgatcg accagcgtgt gctcggcata
     1141 cggcacgcca ccgggcggcc acagcagcgc aagcgcgctc agcagcagcg catcgacccg
     1201 cggcggcggc gccttgggcg cgagctgcgc gcgcagcgcc tgcgccgcgc ccagccagcg
     1261 cagcgcgtgg aagccgagcg cctgcgtgcc gggccgcgcg gcggccgggc agcgcgcgag
     1321 cgcggtgttg agcgactcgc cggcgcgcac cgcctgcacc gcgtcagcgg tgtggttcaa
     1381 gagcttggcg agcggcaggg aggcgggctg ggcgggggcg ctgttcaccg cgcgcagtct
     1441 acggtctgcc ccggcgtcgg ccgccacgac gccctcgctg cggtaccctt gccgcgtgta
     1501 aagctttgaa ggccgcccga tgcgccgcga acaacaaccc acgccgatat tccgcagcgc
     1561 agaggacctg gcgcggctgc cggcctccga acgcatccgc taccggttgg tcggcgccga
     1621 ctgccgcttc cacgcgaacg acaacatctc cgccttcgtc gaggacggcg agatcgacga
     1681 gttgcgcgcc gaggtcgagg ccaagatgca ggaggtgctg cacgcgctgg tgatcgacac
     1741 cgacagcgac cacaacacca acgagaccgc caagcgcgtc gccaagatgt tcctgaccga
     1801 ggtgttcaag ggccgctacg tgccgatccc gccggtcacc gagttcccga acgccgagcg
     1861 cctgaacgag ctgatgatcg tcggcccgat cctggtgcgc agcgcctgct cgcaccacct
     1921 gtgcccgatc atgggcaagg tctggatcgg catcatgccg aacgagcact cgaacctgat
     1981 cggcctgagc aagtactcgc gcatctgcga ctggatcatg agccgcccgc agatccagga
     2041 agaagccgtc acgatgctcg ccgacgaact cgaccggcgc gtcaagcccg acggcctcgc
     2101 ggtcgtgatg gaggccgatc acttctgcat gcagtggcgc ggcgtgaaag acccgcagtc
     2161 gatgatgacg aacagcctga tgcgcggcgc gttcctgaag gacgcggcgc tgcggcgcga
     2221 gttcctgtca ctgctgagcc gcaagacctg aaccgagcgt cgccgcgagc caggcctcgg
     2281 cgcgctcgag cggcgcatcg atcgcgagcc atttgcgccg cgaggtctcg ccgcgcacca
     2341 gcgtgattgc gcgcttgggg caaccgagct cgccggcgag ccaatcgatc agcacctggt
     2401 tcgccttgcc gtcgaccggc ggcgccgcga gccgcacgcg cagtgcgccg tcgtgcaacc
     2461 cgtcggccgc ggtgcgcttg gcgttcggct ggacgctgac cgcgagccac ggcgtcgcgc
     2521 cgtgcagcgt caggcaggcc cagccggccg tcacgccggc gtcttcttgg cggccgcctt
     2581 ctttgccgcg gcaggcacgc gcggcgcgcg cggctcgaac tcgaacgtga ccttgccttc
     2641 cttcttgtcg taggccagcc gcgccttgaa cttgcggcgc gtcttgttcg agacgaagcc
     2701 ttcgag
//
LOCUS       DASRDG010000335         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1312193, whole genome shotgun sequence.
ACCESSION   DASRDG010000335 DASRDG010000000
VERSION     DASRDG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312193"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..613)
                     /locus_tag="VFA35_05565"
     CDS             complement(230..613)
                     /locus_tag="VFA35_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55675.1"
                     /translation="MPDAMTPAWVVYAWAPVLWQASAAPHLHLVHLGTRVLSFGDDDG
                     PCSGQTLWGDRTEDCAAGVAWDWVEVRQGVVAMSDPLGMVTNLRLLDAHGEMLSQTQI
                     AVHLHQLVHALPWQSEVERALRKPS"
     gene            768..1481
                     /locus_tag="VFA35_05570"
     CDS             768..1481
                     /locus_tag="VFA35_05570"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HZT55676.1"
                     /translation="MLQGGFSEVLKSRNRDEFQQELVAFTRRLGFETVSATLVIDHML
                     GEAEFITVDNTPSAFKQSFQNPKKGRHDPVMQHCKRRSVPIIWDQATYVSDGMGEMWE
                     EQARFGYSNGIALALHMPEGRHFFMGVDRDRPVPQDAGELTRVVADLQLFAVHAQDAA
                     QRILTPGPIDPSAPSLTPRELEVLRWTMEGKTAWEVGDVLGIAERTAVLHVSNATHKL
                     GCVNKHQAVLKALRLGLLR"
     gene            1657..1815
                     /locus_tag="VFA35_05575"
     CDS             1657..1815
                     /locus_tag="VFA35_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55677.1"
                     /translation="MQANLQVVDTLKTNTPAPIELTPEQLRQVGGGLGPAGTWSAEST
                     TEGPAGTW"
     gene            1830..3089
                     /locus_tag="VFA35_05580"
     CDS             1830..3089
                     /locus_tag="VFA35_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZT55678.1"
                     /translation="MGSLFRPEALEHRERDWLGSIQLIRPVSLAVLTAFAVAVAVAVG
                     AYLALGEYTRKARVSGYLVPDRGVIRLIAPQPATVVESHVAEGGAVQRGDVLFVLSVE
                     QATPSGDTQAAVQSSIASRALSLQGAARQRSLLEQNQLAAIDRQIDEAQRELDSMKLE
                     SDLQRQRLALAEQARAQYESLRNENFVSAAQVRTKAEEVLNVKAQLQGLERQRSARLR
                     EISALQAQRRELPLQAQSAQGAIDRDLAALAQQAAETEARQRIVVRAPQDGVVSGVIA
                     VPGQTVTPAVALASLLPADAKLQAQLFAPSSAVGFVRANQQVQLRYQAFPYQKFGHQS
                     GEVVQVSRSPLQASELAGLSLPASLSASGEPLYRITVALDRQSVAAYGQPQALSPGMQ
                     LEADVQLDRRRLIEWLFEPVLGIAGRV"
     gene            3105..>3592
                     /locus_tag="VFA35_05585"
     CDS             3105..>3592
                     /locus_tag="VFA35_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HZT55679.1"
                     /translation="MTTGNWLDGLRLGLGGRRLPLILQTEAAECGLACIAMVAAQHGL
                     RSDLPTLRQRFPMSLKGATMADLVRLAGHLQLNARALRAEMAHLPDLVTPCILHWDLN
                     HFVVLKEVSRGVAVIHDPARGVRRLSLAEVSKHFTGVVLELTPQADFRPQTERQTVTL
                     RHL"
BASE COUNT          586 a         1172 c         1253 g          581 t
ORIGIN      
        1 cgatgcggtc gaacgggggc cgcaactgcg gccaggcgac aaggcggtac tctttcatgg
       61 ggccaacgac tcgcttttgt ggtggcaacg ggtctgcgcc gttgctcgac gggagaatgc
      121 agggtaggcg cttatcgacg cggtgtgaag agtttgacgc acgacgcgcg ggaaaatacc
      181 gatggcgaca tgctcacctt tgccgcaggt gcggcgcgaa agcgacggct cacgacggct
      241 tgcgcagcgc gcgttcaact tcgctctgcc acggcagggc atgcaccagc tgatgcaggt
      301 gcacggcgat ctgggtctgg ctcagcatct cgccgtgtgc gtcgagcagc cgcaggttgg
      361 tgaccatgcc gagcgggtcc gacatcgcga ccacgccttg gcgcacctcg acccagtccc
      421 aggccacgcc ggcggcgcag tcttcggtgc gatcgcccca gagcgtttgg ccactgcagg
      481 ggccgtcgtc gtcgccgaaa ctcaacactc tcgtgccgag gtggacaaga tgcaaatgcg
      541 gcgccgcgct ggcctgccac aatacggggg cccacgcgta gacgacccat gcgggcgtca
      601 tggcgtcggg catcgtcgat gcgttgatga ccgaactcct acggattcct gaacgaggcg
      661 tgagtgtgac gaccgaatcc cgtcttgcca tctgtcaacc ttgtcaggta gggtcgctgg
      721 ccatgcaggc ctgtgagttt ttgcgcagtg gggagtgaag gggcggcatg cttcaaggtg
      781 gtttctcaga ggtgctcaag agtcggaatc gcgacgagtt ccagcaggaa cttgtcgcat
      841 tcacgaggcg tctcggcttt gagacggttt cggcgaccct cgtgatcgac cacatgctgg
      901 gcgaggccga gttcatcacg gtcgacaaca cgccatccgc cttcaaacag tccttccaga
      961 accccaagaa gggccggcac gacccggtca tgcagcattg caagcgacgc agcgtgccca
     1021 tcatctggga tcaggcgacg tatgtgagcg acggcatggg cgagatgtgg gaagagcagg
     1081 cccggttcgg ttacagcaac ggcatcgcgc tggcgctgca tatgcctgag ggtcgacact
     1141 tcttcatggg cgtcgaccgc gaccgtccgg ttccgcaaga cgcgggcgaa ttgacacggg
     1201 tcgtggccga cctccagttg tttgcggtgc atgcgcagga tgccgctcag cgcatcctca
     1261 cgcctggccc gatcgacccc agcgctccct cgctcacgcc ccgtgagctc gaagtcctgc
     1321 gttggacaat ggaaggcaag accgcctggg aggttggcga cgtgctcggc atcgccgagc
     1381 ggacagcggt gctgcacgta tccaatgcga cccacaagct gggctgtgtg aataagcacc
     1441 aagctgtgtt gaaagcgctc aggcttggcc tgttgcgtta agaactgcac gccgcgcaag
     1501 cgtgcacgtg gtgttttgtt gcggctttct ccacgccgca atcgatgttg cgtcaatccc
     1561 caccctgtaa gacttgacag ttacgcgatt gacttgctcc tgctgcaata ggttcagtgc
     1621 atgcgcggcg tgcacctacc acccacggag cacagcatgc aagcgaatct tcaagtcgtc
     1681 gacaccctca agaccaacac cccggctccc atcgagctga ccccggaaca actgcgtcaa
     1741 gtcggcggtg gcctcggccc ggcgggcact tggtccgctg aaagcacgac cgagggcccg
     1801 gccggcacct ggtaagcagg tttccgccgg tgggcagcct gttccgcccc gaggccctcg
     1861 aacaccgcga acgcgactgg ctgggcagca ttcagttgat ccggccggtt tcgctggcgg
     1921 ttctgaccgc gttcgcggtg gcggtggcgg tggcggtcgg cgcctacctg gcgctgggcg
     1981 agtacacgcg caaggcgcgg gtcagcggct acctggtgcc cgaccgcggc gtgatccggc
     2041 tgattgcgcc gcagccggcg accgtggtcg agagccacgt cgccgaaggc ggcgcggtgc
     2101 agcgcggcga cgtgctgttc gtgctgtcgg tcgagcaggc cacgccgagc ggcgacaccc
     2161 aggcggcggt tcagagcagc atcgcgtcgc gcgcgctgag cttgcagggc gcggcgcggc
     2221 agcgcagcct gctcgagcag aaccagctcg cggcgatcga tcgccagatc gacgaggcgc
     2281 agcgcgaact ggactcgatg aagctcgagt ccgacttgca gcgccagcgt ctggcgctgg
     2341 ccgagcaggc gcgggcgcag tacgagtcgc tgcgcaacga gaacttcgtc tccgcggcgc
     2401 aggtgcggac gaaggccgag gaggttctca acgtcaaggc gcagttgcag ggcctggagc
     2461 gccagcgaag cgcgcggctg cgcgagatct cggcgctgca ggcacagcgc cgcgagttgc
     2521 cgctgcaggc gcagagcgcg caaggggcga tcgaccgcga cctggccgcg ctcgcccagc
     2581 aggcggccga aaccgaggcg cgccagcgca tcgtcgtgcg cgcgccgcag gacggcgtgg
     2641 tcagcggcgt gatcgcggtg ccgggccaga ccgtcacgcc ggcggtcgcg ctggcgagcc
     2701 tgctgccggc cgacgcgaag ttgcaggcgc agctgttcgc gccctcgagc gcggtcggct
     2761 tcgtgcgcgc gaaccagcag gtgcagctgc gctaccaggc cttcccgtac cagaagttcg
     2821 gccaccagtc gggcgaggtg gtgcaggtgt cgcgctcgcc gctgcaggcc tccgagcttg
     2881 ccggcctgtc gctgccggcg tcgctgagcg ccagcggcga gccgctctat cgcatcaccg
     2941 tggcgctcga tcgacagagc gtggccgcct acggccagcc gcaggcgctg tcacccggca
     3001 tgcagctcga agccgatgtg cagctcgatc ggcgccggct gatcgagtgg ctgttcgagc
     3061 cggtgctggg catcgcgggc cgggtctgac gcgatggccg caccatgacc acaggcaact
     3121 ggctcgacgg gctgcggctc ggcctcggcg ggcgtcggct gccgctgatc ctgcagaccg
     3181 aggccgccga gtgcggcctg gcctgcatcg cgatggtggc cgcgcagcac gggctgcgca
     3241 gcgacctgcc gacgctgcgc cagcgctttc cgatgtcgct gaagggtgcg acgatggccg
     3301 atctggtgcg cctggccggc cacctgcagt tgaacgcgcg cgcactgcgc gccgagatgg
     3361 cgcacctgcc cgatctggtg acgccctgca tcctgcactg ggacctgaac cacttcgtcg
     3421 tgctgaagga agtgagccgc ggcgtcgcgg tgatccacga cccggcgcgc ggcgtgcggc
     3481 gcctgtcgct ggccgaggtc tcgaagcatt tcaccggcgt ggtgctcgag ctgacgccgc
     3541 aggccgactt ccggccgcag accgaacgcc aaacggtcac cttgcggcat ct
//
LOCUS       DASRDG010000336         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1434533, whole genome shotgun sequence.
ACCESSION   DASRDG010000336 DASRDG010000000
VERSION     DASRDG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434533"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2255)
                     /locus_tag="VFA35_05590"
     CDS             complement(<1..>2255)
                     /locus_tag="VFA35_05590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745752.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZT55680.1"
                     /translation="KPFIFSRLEAMGIATTIKAAKKEVESGTPVVWDILEEVIKEHPV
                     LLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLSIEAQME
                     ARTLMLATNNILFPANGEPSIVPSQDVVLGLYYATRERINAKGEGIIFSDIGEVQRAL
                     DNGVVEITTKISVRLTEYTKNKETGEFTPETKLVDTTVGRALLSEILPKGLPFSNINK
                     ALKKKEISRLINVSFRKCGLKETVVFADKLLQSGFRLATRAGISIAIDDMLVPKEKHG
                     LIERAEKEVKEIEQQYVSGLVTAGERYNKVVDIWGKTGDEVGKVMMSQLSKQKTIDRH
                     GKTVDQESFNSIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLN
                     VLQYFISTHGARKGLADTALKTANSGYLTRRLVDVTQDLVVIEDDCGTQNGMNMRALV
                     EGGEVIESLRDRVLGRVTAIEVQHPETQETLLEAGTLMDEDILDTLEAAGVDEVKVRT
                     ALTCGTRFGICAKCYGRDLGRGGLVNMGEAIGVIAAQSIGEPGTQLTMRTFHIGGAAS
                     RAAVASSVDAKSDGIVGFNATMRYVTNGKGELVVISRSGEIIISDQHGRERERHKVPY
                     GALLSIKADQQVKAGTVLANWDPLTRPIITEFAGKAKFENVEEGVTVAKQLDEVTGLS
                     TLVVIDPKRRGAAKVVRPQVKLLDVAGNEVKIPGTDHSVTIAFQVGSLIQIRDGQDLA
                     PGEVLARIPVEG"
BASE COUNT          328 a          762 c          717 g          448 t
ORIGIN      
        1 ccttcgaccg ggatgcgcgc cagcacttcg cccggcgcca ggtcttgccc gtcgcggatc
       61 tggatcagcg agccgacctg gaacgcgatc gtcaccgagt ggtcggtgcc cgggatcttg
      121 acctcgttgc cggccacgtc gagcagcttg acctgcgggc gcacgacctt ggcagcgccg
      181 cggcgcttcg ggtcgatcac caccagggtc gacaggccgg tgacctcgtc gagctgcttg
      241 gccacggtga cgccttcctc gacgttctcg aacttcgcct tgccggcgaa ctcggtgatg
      301 atcgggcggg tcagcggatc ccagttggcg agcacggtgc cggccttgac ctgctgatcg
      361 gccttgatgc tgagcagcgc gccgtagggc accttgtgac gttcgcgctc gcgaccgtgc
      421 tggtcggaga tgatgatctc gccggatcgc gaaatcacca ccagctcgcc cttgccgttg
      481 gtcacgtagc gcatcgtcgc gttgaagccg acgatgccgt cgctctttgc gtcgacgctc
      541 gaagccaccg ccgcacgcga cgccgcacca ccgatgtgga aggtgcgcat cgtcagctgc
      601 gtgcccggct cgccgatcga ctgggccgcg atcacgccga tcgcctcgcc catgttgacc
      661 aggccgccgc ggccgagatc gcggccgtag cacttggcac agatgccgaa gcgcgtgccg
      721 caggtcagcg cggtgcggac cttgacctcg tcgacgcccg ccgcttccag cgtgtcgagg
      781 atgtcttcgt ccatcagcgt gccggcctcg agcagcgtct cctgcgtctc cgggtgctgc
      841 acctcgatcg cggtgacgcg gccgagcacg cggtcgcgca gcgactcgat cacctcgccg
      901 ccctcgacca gcgcgcgcat gttcatgccg ttctgggtgc cgcagtcgtc ctcgatcacg
      961 accaggtcct gcgtcacgtc gaccaggcgg cgcgtcaggt agcccgagtt cgcggtcttc
     1021 agcgcggtgt ccgcgaggcc cttgcgggcg ccgtgggtcg agatgaagta ctgcagaacg
     1081 ttcaggcctt cgcggaagtt cgcggtgatc ggcgtctcga tgatcgagcc gtcaggcttg
     1141 gccatcaggc cgcgcatgcc ggcgagctgg cggatctgcg cggcggagcc acgcgcgccc
     1201 gagtcggcca tcatgtagat cgagttgaac gactcctggt ccaccgtctt gccgtggcga
     1261 tcgatcgtct tctgcttcga cagctggctc atcatgacct tgccgacctc gtcgccggtc
     1321 ttgccccaga tgtcgaccac cttgttgtaa cgctcaccgg cagtcaccag acccgacacg
     1381 tactgctgct cgatctcttt cacttccttc tcggcgcgct cgatcaggcc gtgcttctcc
     1441 ttcggcacca gcatgtcgtc gatcgcgatc gagatgccgg cgcgcgtggc caggcggaac
     1501 ccgctttgca gcagcttgtc ggcgaacacg accgtctctt tcagcccgca cttgcggaac
     1561 gagacattga tcaggcgcga aatctccttc ttcttcagcg ccttgttgat gttgctgaac
     1621 ggcaggccct tcggcaggat ttcggacagc agggcgcggc cgaccgtggt gtccacgagc
     1681 ttggtttccg gcgtgaactc gccggtctcc ttgttcttcg tgtactcggt caggcgcacg
     1741 ctgatcttcg tggtgatctc gaccacgccg ttgtcgagcg cgcgctgcac ttcgccgatg
     1801 tcggagaaga tgatgccttc gcccttcgcg ttgatgcgct cgcgcgtggc gtagtacagg
     1861 cccagcacca cgtcctgact cggcacgatc gacggttcgc cgttcgccgg gaacaggatg
     1921 ttgttggtcg ccagcatcag cgtgcgggct tccatctgcg cctcgatcga cagcggcacg
     1981 tgcacggcca tctggtcacc gtcgaagtcg gcgttgaacg ccgcgcagac gagcgggtgc
     2041 agctggatcg ccttgccttc gatcagcacc ggctcaaagg cttgaatgcc caggcggtgc
     2101 aaggtcggcg cgcgattcaa caggaccggg tgctccttga tgacttcttc gaggatgtcc
     2161 cagaccaccg gcgtgccgga ctcgacttcc ttcttcgccg ccttgatcgt cgtcgcgatg
     2221 cccatggctt cgaggcgcga gaagatgaac ggctt
//
LOCUS       DASRDG010000337         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1657698, whole genome shotgun sequence.
ACCESSION   DASRDG010000337 DASRDG010000000
VERSION     DASRDG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657698"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VFA35_05595"
     CDS             complement(<1..116)
                     /locus_tag="VFA35_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZT55681.1"
                     /translation="MTSVQTAFWVVALLVVLAVAFDFMNGFHDAANAIATVVS"
     gene            complement(220..867)
                     /locus_tag="VFA35_05600"
     CDS             complement(220..867)
                     /locus_tag="VFA35_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HZT55682.1"
                     /translation="MLFGKLLPREGNFFELFNQHGAFIVAGARSFILLIQNYGDLGLR
                     EKYANEVDTAERDADRITAEVNRLLHKTFITPIDREQIHGLINAMDDIVDLLQDASET
                     MALYDVRAMTEDILRLGDISAKCCERVQHAVSLLPKISQAETAEAAIKTCQEIDRLES
                     DADRVMRSAMSKLFREETDVRELIKLKAIYELLESISDRCEDVANLIEGVVLENS"
     gene            complement(949..1617)
                     /locus_tag="VFA35_05605"
     CDS             complement(949..1617)
                     /locus_tag="VFA35_05605"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HZT55683.1"
                     /translation="MDDSPALRRFRYIAIEGPIGVGKSTLARKLAAHLGADLLLEQAQ
                     ENPFLDRFYKDMPGYAFQTQLAFLFQRVKQMQTVAQPGMFANAVVSDYLFAKDAIFAR
                     LNLSDDEFRLYSQMYAQVVPQVREPDLVIWLQAAPPTLLRRIHRRAIPMEQGIAPDYL
                     QRLCDAYVEYFHSYDGAPVFALGTERFNPVERDADFALLLERLAAFRGRREFFNSHVE
                     IRLG"
     gene            complement(1617..1973)
                     /locus_tag="VFA35_05610"
     CDS             complement(1617..1973)
                     /locus_tag="VFA35_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HZT55684.1"
                     /translation="MLNAIPVSLQTVLLLAASNVFMTVAWYGHLKNLADKPWYWAAII
                     SWGIALFEYLLQVPGNRIGFAGGISLAQLKITQEVITLAVFVPFACVYMDQPFRLDFV
                     WAGLCMVGAVYFIFRA"
     gene            complement(1967..2428)
                     /gene="folK"
                     /locus_tag="VFA35_05615"
     CDS             complement(1967..2428)
                     /gene="folK"
                     /locus_tag="VFA35_05615"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZT55685.1"
                     /translation="MGGNLGDVRGSLDAAFAALSALPETRLLRTSSLYASAPHDASGP
                     DYLNAVAELSTTLAADDLLAALQAIETNAGRARPYRNAPRTLDLDLLLYGARRTASGS
                     LVVPHPRLHERAFVLRPLAEIAPRLVVPGLGRLADLLPHVAGQRADRLPPC"
     gene            complement(2538..2978)
                     /locus_tag="VFA35_05620"
     CDS             complement(2538..2978)
                     /locus_tag="VFA35_05620"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HZT55686.1"
                     /translation="MKLKPIALSALLALASASGFAQSFSSGTTGSFSYLVDEGASLTA
                     SVTSVITAGLGFDIDSVLFGSTPFAFTSTAVGGSTFEQYTFSAASLAAGLYTIYVTGT
                     GTAGSAYTGNVVITSPVPEPETSALMFAGLGAIGFMTSRRQRVP"
     gene            complement(3183..>3366)
                     /locus_tag="VFA35_05625"
     CDS             complement(3183..>3366)
                     /locus_tag="VFA35_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HZT55687.1"
                     /translation="AAAASAGDADPRAPAPDAAVAPTEGDAPKKRRRRRRKPAPEGAE
                     PAALADTAPPNPSRDF"
BASE COUNT          571 a         1118 c         1161 g          516 t
ORIGIN      
        1 gagacgacgg tggcgatcgc gttggccgcg tcgtggaagc cgttcatgaa gtcgaacgcg
       61 accgccagca cgacgagcag cgcgacgacc cagaaggcgg tttgaacaga agtcatgaca
      121 gcccctcgcc tgcggagtac cgcagacgtt cccccgaggg gatgcgggcc ggcttggggc
      181 ggcccggcgc tcggcccgcc gaagtcagca agctcatcat cacgagttct cgagaaccac
      241 gccttcgatc aggttcgcca cgtcctcgca gcggtcggag atcgactcca gcagctcgta
      301 gatcgccttc agcttgatca gctcgcgcac gtcggtctct tcgcggaaca gcttcgacat
      361 cgccgagcgc atcacccggt cggcgtcgga ttcgagcctg tcgatctctt ggcaggtctt
      421 gatcgcggcc tcggcggtct cggcctggct gatcttcggc agcagcgaca ccgcgtgctg
      481 cacgcgctcg cagcacttgg cgctgatgtc gccgaggcgc aggatgtctt ctgtcatcgc
      541 gcgcacgtcg tagagcgcca tggtttccga agcgtcctgc agcaggtcga cgatgtcgtc
      601 catcgcgttg atcaggccgt ggatctgctc gcggtcgatc ggcgtgatga aggtcttgtg
      661 cagcagccgg ttcacttcgg cggtgatgcg gtcggcgtcg cgctcggcgg tgtcgacctc
      721 gttcgcgtac ttctcccgca accccaggtc gccgtagttc tggatcagca ggatgaacga
      781 gcgcgcgcct gcgacgatga acgcgccatg ctgattgaac agctcgaaga aattgccttc
      841 gcgaggcagc agtttgccga acagcatggg gatcctcttc cgcggcggcc cgcgtgtgtc
      901 acgaaaccgt catcaaatcg actcgggcgg gagtgtactt ggcgggattc agccgagccg
      961 gatctcgacg tgcgaattga agaactcgcg ccggccgcga aacgcggcga gccgctccag
     1021 cagcagcgcg aaatcggcgt cgcgttcgac cgggttgaag cgctcggtgc cgagcgcgaa
     1081 caccggcgcg ccgtcgtagc tgtggaagta ctcgacatac gcgtcgcaca gccgctgcag
     1141 gtagtcgggc gcgatgccct gctccatcgg gatcgcgcgg cggtggatgc gccgcagcag
     1201 cgtcggcggc gcggcctgca gccagatcac caggtcgggc tcgcgcacct gcggcaccac
     1261 ctgcgcgtac atctgcgaat acaggcggaa ctcgtcgtcg ctcaggttca ggcgcgcgaa
     1321 gatcgcgtcc ttcgcgaaca ggtagtcgct gacgaccgcg ttcgcgaaca tgcccggctg
     1381 cgccaccgtc tgcatctgct tgacgcgctg gaacaggaac gcaagctggg tctggaacgc
     1441 gtagcccggc atgtccttgt agaaccggtc gagaaacggg ttctcctgcg cttgctcgag
     1501 cagcaggtcg gcgcccagat gcgccgcgag cttgcgcgca agcgtgctct tgccgacgcc
     1561 gatcggtccc tcgatcgcga tgtagcgaaa gcgtcgcagc gcgggcgagt cgtccatcag
     1621 gcgcggaaga tgaagtagac cgcgccgacc atgcacaggc cggcccagac gaagtcgagc
     1681 ctgaacggct ggtccatgta cacacacgcg aacggcacga acacggccaa ggtgatcacc
     1741 tcctgcgtga tcttcagctg cgccaggctg atgccgccgg cgaagccgat gcggttgccc
     1801 ggcacctgca gcaggtactc gaacagcgcg atgccccagc tgatgatcgc ggcccagtac
     1861 caaggcttgt cggccaggtt cttcaggtgg ccgtaccagg cgacggtcat gaagacgttc
     1921 gacgccgcca gcagcagcac ggtttgcaac gagaccggga tggcgttcaa catggcggca
     1981 gtctatcggc gcgctggccg gcgacgtgcg gcaacagatc ggccagccgg ccgagcccgg
     2041 gcacgacgag ccgcggcgcg atctcggcca gcgggcgcag cacgaaggca cgttcgtgca
     2101 ggcggggatg cggcacgacc aagctcccgc tggcggtgcg ccgcgcccca tagagcagca
     2161 ggtccagatc cagcgtgcgc ggtgcgttgc gatacgggcg cgcgcgcccg gcattcgtct
     2221 cgatcgcctg caaggccgcg agcagatcgt ctgcggccag cgtggttgac aactcggcca
     2281 ccgcgttcag gtagtccggg cccgacgcat cgtgcggcgc cgacgcgtac aacgacgatg
     2341 tgcgcaacag ccgcgtctcg ggcagcgcgc tgagcgccgc gaaggccgca tcgagcgacc
     2401 cacgcacatc gcccaggttg ccgcccatgc cgacgaacgc ccgaacagcg cggctcacag
     2461 cgagagatcg gcgtgacgag gcatgccccg attgtggcgg ctcgccacgc cgtcggccgc
     2521 agagcggccc gatgggctca gggcacgcgc tggcggcggc tggtcatgaa gccgatcgcg
     2581 ccgaggccgg cgaacatgag tgcggaggtt tcgggttccg gcacgggcga ggtgatcacg
     2641 acgttgccgg tatacgcact gccggccgtg ccggtaccgg tgacgtagat cgtgtacaga
     2701 cccgccgcca acgaagccgc cgagaacgtg tactgctcga aggtcgagcc gcccacggcg
     2761 gtgctcgtga acgcgaacgg cgtcgacccg aacagcaccg agtcgatgtc gaaacccaat
     2821 ccggcggtga tcaccgaagt gaccgaggcc gtcaggctcg cgccttcgtc gaccaggtaa
     2881 gagaacgagc ccgtggtacc gctcgagaac gactgcgcga agccgctggc ggaagccagg
     2941 gcgagcagtg ctgacagggc gatcggtttc agtttcatgc ctaactcctt gcttgacact
     3001 cggaaggcac cggcccgttc tggcaggccg gcacagctgg accgacgtgc tcgtggcgcg
     3061 ccggtcgccg ggcgtctagc aagttgcgcg ccaaaaccaa gggtaaaccc cgatctggtg
     3121 caagcccttt cgagccgaca cacactgccc gctcgcggtg cgggcagggt cggcggcggc
     3181 cctcagaaat cgcgcgatgg attcggcggc gcggtgtcgg ccagcgccgc cggctcggcg
     3241 ccctcgggcg ccggcttgcg gcgacggcgg cgtcgcttct tcggcgcgtc gccttcggtc
     3301 ggcgccacgg cggcgtcggg cgccggggcg cgcgggtccg cgtcgcccgc gctcgccgcc
     3361 gcggcc
//
LOCUS       DASRDG010000338         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_856512, whole genome shotgun sequence.
ACCESSION   DASRDG010000338 DASRDG010000000
VERSION     DASRDG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856512"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFA35_05630"
     CDS             complement(<1..226)
                     /locus_tag="VFA35_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HZT55688.1"
                     /translation="MKVSIFGSGYVGLVTGACLAEAGNHVLCVDVDTQKVARLNAGEV
                     PIHEPGLDALIRRNREGGRLEFTTDGARAVD"
     gene            complement(254..814)
                     /locus_tag="VFA35_05635"
     CDS             complement(254..814)
                     /locus_tag="VFA35_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZT55689.1"
                     /translation="MRNGQTWVRVVVMVGILAASAAGLEAVRAAGPPPASTGGSTAAE
                     RLSAQEEQLRITLAPTAAVVVRSEKSLELWYPVRLAFMPDGNELLPTGATLLDLVARS
                     LRDYTHSEVVVAVYTDAIGSSDYNQSQSQARAAVLVEELEARGVAAARLVARGMGESA
                     QLEAPKTPEGRDLNRRVQLVITPLSS"
     gene            910..1998
                     /gene="gmd"
                     /locus_tag="VFA35_05640"
     CDS             910..1998
                     /gene="gmd"
                     /locus_tag="VFA35_05640"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZT55690.1"
                     /translation="MGKTALVTGITGQDGAYLAELLLEKGYEVHGIKRRASSFNTDRI
                     DHLYQDPHERNVRMRLHYGDLTDATNLIRIVQQVQPDEIYNLAAQSHVAVSFETPEYT
                     ANADALGSLRLLEAIRILGLEKRTRYYQASTSEMYGKVQEVPQRETTPFYPRSPYGAA
                     KVYAYWITVNYREAYGLYACNGILFNHESPVRGETFVTRKITRALARISLGLQECLYL
                     GNMDSLRDWGHAKDFVLAQWLMLQQDQPEDFVIATGEQHSVREFVERAGARLGMKLEW
                     RGRGLQEQGVDTATGRAIVKVDPRYFRPTEVDSLLGDATRARTRLGWQPKIGFEQLVA
                     DMVAGDLEHARRDAVVAREGFQTYRRHE"
     gene            1991..>2220
                     /locus_tag="VFA35_05645"
     CDS             1991..>2220
                     /locus_tag="VFA35_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZT55691.1"
                     /translation="MNSDARIFVAGHRGLVGSAVVRRLRALGYSNLLLAERRELELTR
                     QSHVEEWFAAHRPEVVIMAAAKVGGIHANNTYP"
BASE COUNT          385 a          770 c          703 g          362 t
ORIGIN      
        1 ggtcgacagc gcgcgcgccg tcggtcgtga actcgagtcg tccgccttcg cggttgcggc
       61 gaatcagcgc atcgagcccc ggttcgtgga tcggcacttc gcccgcattc aacctcgcga
      121 ctttctgcgt gtcgacgtcg acgcacagca cgtggttgcc ggcctcggcc aggcaggctc
      181 ccgtgaccag gcccacgtat cccgacccga aaatgctgac tttcatgagg cgcgcaagcc
      241 taacggatgg tcatcaggat gacaaaggcg tgatcacgag ttggaccctg cggttgagat
      301 cgcgaccttc cggcgtcttc ggggcctcga gctgcgccga ttcgcccatg ccgcgggcca
      361 cgagtcgcgc ggcggcgacg ccgcgggctt ccagttcctc caccaggacc gccgcgcgcg
      421 cttgcgactg gctctggttg tagtcgctgc tgccgatggc atcggtatac acggcgacga
      481 cgacttcgga gtgcgtgtag tcacggagtg agcgggcgac gagatcgagc aacgtcgcgc
      541 ccgtgggcag cagttcgttg ccgtccggca tgaaggcgag ccgcaccgga taccagagct
      601 cgagtgactt ttccgagcgc acgaccacgg ccgcggtcgg cgccagcgtg atgcgcaatt
      661 gctcctcctg ggccgagagc cgctcagcgg ccgtcgaccc accggttgag gctggcggcg
      721 gtcccgccgc gcgcaccgct tccaacccgg ccgcactggc ggccaggatt ccaaccatca
      781 ctacgacgcg tacccatgtc tgcccgttgc gcattaccac ctgctcctgc agctattgtg
      841 cggatttcga tccgccgggc caatagtcga cgtaaactta agccccggac ggctttcgag
      901 gacaggccct tgggcaagac agctctcgtt actggaatca ccggccagga tggcgcttac
      961 ctggcggaat tgctgctgga aaagggctac gaggtgcacg gcatcaagcg ccgcgcctcg
     1021 agcttcaaca ccgatcgcat cgatcacctc taccaggatc cgcacgaacg caacgtgcgt
     1081 atgcgcctgc actacggcga cctgaccgat gccaccaacc tgatacgcat cgtgcaacag
     1141 gtgcagcccg acgaaattta caacctggcc gcgcagagcc acgttgcggt ttccttcgag
     1201 acccctgaat acaccgccaa tgccgacgcg ctgggctcgc tgcgcctgct cgaggcgatc
     1261 cgcatcctcg gactcgagaa gcgcacccgc tactaccagg cctcgacctc ggagatgtac
     1321 ggcaaggtgc aggaggtgcc gcagcgcgag accaccccgt tctacccgcg ctcgccctac
     1381 ggcgccgcca aggtctacgc ctactggatc acggtcaact accgcgaggc ctacggcctc
     1441 tatgcctgca atggcatcct gttcaatcac gaatcgccgg tgcgtggcga aaccttcgtg
     1501 acgcgcaaga ttacgcgcgc gctcgcgcgc atctcgctcg gcctgcagga atgcctgtac
     1561 ctcggcaaca tggactcgct ccgtgactgg ggacacgcca aggacttcgt gctggcgcaa
     1621 tggctcatgc tgcagcagga ccagcccgag gatttcgtga tcgccaccgg ggagcagcat
     1681 tcagtgcgcg agttcgtcga gcgcgcgggc gcgcggctcg gcatgaagct cgagtggcgc
     1741 ggccgcggcc tccaggaaca gggcgtcgat accgccaccg ggcgggccat cgtcaaggtc
     1801 gatccgcgct acttccgccc caccgaggtc gattcactgc tcggcgatgc cacgcgcgcg
     1861 cgcacgaggc tcggctggca gccgaagatc ggcttcgagc agctggtcgc cgacatggtg
     1921 gcgggcgacc tcgagcacgc gcgccgcgac gcggtcgtcg cccgcgaagg gttccagacc
     1981 tatcggcgcc atgaatagcg acgcgcgcat cttcgtcgcc ggccaccgcg gactggtggg
     2041 ctcggcggtg gtgcgcagac tccgcgccct ggggtattcg aacctgctgc tcgccgaacg
     2101 ccgcgagctc gagctgacgc gccagtccca cgtcgaggaa tggttcgccg cgcaccgacc
     2161 cgaagtcgtc atcatggcgg cggccaaggt gggcggcatc cacgcgaaca acacctaccc
//
LOCUS       DASRDG010000339         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1312930, whole genome shotgun sequence.
ACCESSION   DASRDG010000339 DASRDG010000000
VERSION     DASRDG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312930"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="VFA35_05650"
     CDS             <1..1677
                     /locus_tag="VFA35_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZT55692.1"
                     /translation="IELQGEVQMTSRLEAAKAASQAFDPLEFDRFTRFQELTRMMAES
                     VNDVATVQRTLQRSLEVTEDELVAQARLTRDLQDDLLRTRMVEFEGLSDRLYRVVRQA
                     AKETGKLVRLDIVGGRVEVDRGVLDRMTGAFEHLLRNCVGHGIELPEARTRAGKDATG
                     SIIVALSHEGNEVGVEFRDDGAGLDLPRIRDKGLALGLLDPNQPATDAELANLIFAPG
                     FSTAETVTELSGRGVGMDVVRSEVNAMGGRVETATSAGQGTSFKLILPLTTAVTQVVM
                     LRCGDTNVAVPSTLVEVVRRATPAEIDAAYASGAYPMGDTLLQFFWLGALLQISARPA
                     DSEARTRAVVVIRSAQQRIALHVDEVLGNQEVVVKHLGPQLSRLPGLAGITLLASGAV
                     ALIYNPVALATLYGEAAREATLAALRAPGAHTVAQEPAAAEAPLVMVVDDSLTVRRVT
                     QRLLQREGYRVVLAKDGLDALERLAEDVPQIVLSDIEMPRMDGFDLVRNMRADARLRD
                     LPVIMITSRIAQKHREYAAELGVDHYLGKPYSEEDLLALVARYTRDRAEA"
     gene            1782..>2591
                     /locus_tag="VFA35_05655"
     CDS             1782..>2591
                     /locus_tag="VFA35_05655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZT55693.1"
                     /translation="MSPLEKFLLFVAAPAVFVFALIEAVVLARRGRYDWRAFGVSAFD
                     LVARIAVGIVLPLSIAAPLVEWAVRHRLTTIPLDDWSAIVLLFVGQEFCYYWYHRAAH
                     RVRWFWGNHAVHHSPNELNLSAAFRIGAFGRLSGTTLFFVPLVWLGFDVRTVFAVLTL
                     NLLYQFWIHATWIPKLGWLEYVLNTPSAHRVHHASNLEYLDANYGGVLIVFDRLFGTY
                     VAERDDLPCRYGLVHPLTTYNPLKVEFAEWIGLARDLAKARSLHAVLGHLFM"
BASE COUNT          365 a          923 c          901 g          402 t
ORIGIN      
        1 atcgagctcc agggcgaggt gcagatgacc tcgcggctcg aggccgccaa ggccgcctcg
       61 caggcgttcg atccgctcga gttcgaccgc ttcacccgct tccaggaact gacgcggatg
      121 atggccgagt cggtcaacga cgtcgccacg gtgcagcgca ccctgcagcg ctcgctcgaa
      181 gtgaccgagg acgaactggt cgcgcaggcg cgcctgacgc gcgacctgca ggacgacctg
      241 ctgcgcaccc gcatggtcga gttcgagggc ctctcggacc gcctgtatcg cgtcgtgcgc
      301 caggccgcga aggaaaccgg caagctggtg cggctcgaca tcgtcggcgg ccgggtcgag
      361 gtcgaccgcg gcgtgctcga ccgcatgacc ggtgcgttcg agcacctgct gcgcaactgc
      421 gtgggccacg gcatcgagct gcccgaggca cgcacccgcg ctggcaagga cgcgaccggc
      481 tcgatcatcg tcgcgctctc gcacgagggc aacgaagtcg gcgtcgagtt ccgcgacgac
      541 ggcgcgggcc tggacctgcc gcgcatccgc gacaagggcc tggcgctcgg cctgctcgac
      601 ccgaaccagc ccgccaccga cgccgagctt gcgaacctga tcttcgcgcc cggcttctcg
      661 accgccgaga cggtgaccga gctctcgggc cgcggcgtcg gcatggacgt ggtgcgctcc
      721 gaagtcaacg cgatgggcgg ccgggtcgag accgcgacct cggccggcca gggcacctcg
      781 ttcaagctga tcctgccgct caccaccgcg gtcacgcagg tcgtgatgct gcgctgcggc
      841 gacaccaacg tggcggtgcc gtcgacgctg gtcgaagtgg tgcgccgcgc gacgccggcc
      901 gagatcgacg ccgcctacgc gagcggcgcg tacccgatgg gcgacacgct gctgcagttc
      961 ttctggctcg gcgcgctgct gcagatcagc gcgcggccgg ccgacagcga ggcgcgcact
     1021 cgcgcggtcg tcgtgatccg cagcgcgcag cagcgcatcg cgctgcacgt cgacgaagtg
     1081 ctgggcaacc aggaagtcgt ggtcaagcac ctcgggccgc agctgtcgcg gctgccgggc
     1141 ctggccggca tcacgctgct cgcctcgggc gcggtcgcgc tgatctacaa cccggtcgcg
     1201 ctcgccacgc tctacggcga ggccgcgcgc gaggcgacgc tggccgcgct gcgcgcgccc
     1261 ggcgcacaca cggtcgcgca ggagccggcg gcggccgaag cgccgctggt gatggtcgtc
     1321 gacgactcgc tgacggtgcg ccgcgtcacg cagcgcctgc tgcagcgcga gggctaccgc
     1381 gttgtcctcg cgaaagacgg tctggacgcg ctcgagcgcc tcgccgagga cgtgccccag
     1441 atcgtgctgt cggacatcga gatgccgcgc atggacggct tcgacctggt gcgcaacatg
     1501 cgcgccgacg cgcggctgcg cgacctgccg gtgatcatga tcacctcgcg gattgcgcag
     1561 aagcaccgcg agtacgcggc cgagctcggc gtcgatcact acctgggcaa gccgtactcc
     1621 gaagaagacc tgctggcgct cgtcgcgcgc tacacgcgcg atcgcgccga ggcctgagaa
     1681 ggtgtgagcg cacggccgaa gcgcgcggcc cgcggctttc ggcgagacgt ggcggcggcg
     1741 gcgcgatatc gttcgggctc cggtccagcg ccgcgcacac catgagcccc ctcgagaaat
     1801 tcctgctgtt cgtcgccgcg cccgcggtgt tcgtcttcgc gctgatcgag gccgtcgtgc
     1861 tggcccggcg cgggcgctac gactggcgtg cgttcggcgt ctcggcgttc gacctggtcg
     1921 cgcgcatcgc agtgggcatc gtgctgccgc tgtcgatcgc ggcgccgctg gtggaatggg
     1981 ccgtccggca tcggctgacg acgatcccgc tcgacgattg gagcgccatc gtgctgctct
     2041 tcgtcggcca ggagttctgc tactactggt accaccgtgc cgcgcaccgg gtgcgctggt
     2101 tctggggcaa tcatgcggtg caccactcgc cgaacgaact gaacctctcg gccgcgttcc
     2161 gtatcggcgc cttcggcagg ctgtccggca cgaccctgtt cttcgttccg ctcgtctggc
     2221 tcggcttcga cgtgcgcacg gtgttcgcgg tgctgacgct gaacctgctg taccagttct
     2281 ggatccacgc gacctggatc ccgaagctcg gctggctcga atacgtgctc aatacgccgt
     2341 cggcgcatcg cgtgcaccac gcctcgaacc tcgaatacct ggacgcgaac tacggcggcg
     2401 tgctgatcgt gttcgaccgg ctgttcggca cctacgtggc cgagcgcgac gacctgccgt
     2461 gccgctacgg gctggtgcac ccgctgacca cctacaaccc gctgaaggtc gagttcgccg
     2521 agtggatcgg cctggcgcgc gacctcgcca aggctcgcag cctgcacgcg gtgctcggcc
     2581 acctgttcat g
//
LOCUS       DASRDG010000340         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_623895, whole genome shotgun sequence.
ACCESSION   DASRDG010000340 DASRDG010000000
VERSION     DASRDG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623895"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFA35_05660"
     CDS             <1..881
                     /locus_tag="VFA35_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZT55694.1"
                     /translation="NPTETIREALPADPAALRTAARSVYRHRLPVRVMHWINVICLTI
                     LLMSGLQIFNAHPALYWGKDSSFAQPWVAIGALNAPGGGVTGVTTIAGHRFSTDGVLG
                     ASKVGGEGAKVVRAFPSWATIPGPQWLSMGRHWHFFFAWLFVLNGIAYVAYAIASGHL
                     TRDLLPTRAELRQIGRSIKDHLLFRHETGAAATRYNVLQNLAYLAVIFGLLPLVIVAG
                     MAMSPRLDAVAAGWVDLLGGRQSARTLHFLAATGLLLFVLVHLFEVLINGAWNQIRSM
                     LTGWYVLPVDDKRTKP"
     gene            878..1687
                     /locus_tag="VFA35_05665"
     CDS             878..1687
                     /locus_tag="VFA35_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZT55695.1"
                     /translation="MIRKPISSGQPKAAASRRTALRAALAGLGAASLAGCDRLSNNAA
                     FVDVLKSTQHLSHGAQRLVAGRKAMAQEFSQADVAAHFKANGTLAPRDADYQALMKDG
                     FADWKLQVGGLVDAPAAFTLAELRAMPSRTQITRHDCVEGWSTIGKWKGVPLSHLLAL
                     VKPRPQAKFVVFRCADSMEDPNASADSRYYESVDMDDAYHVQTVLAYELNDAPLPVAN
                     GAPLLARIERQLGYKHAKYLMRVELVERFDQIRGGQGGYWEDNGYEWYAGI"
     gene            1741..2127
                     /locus_tag="VFA35_05670"
     CDS             1741..2127
                     /locus_tag="VFA35_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZT55696.1"
                     /translation="MRKLFRSFLVFIALGFAALAAGAAELPFDKAKLDAALAQRKPVI
                     VHFAADWCPTCKEQKPIVAALMQEPKRQDIALFVADFDKETALKKRLKVTMQSTFVVF
                     KGGKEVGRSTGQTDPAELAAVFDKAL"
     gene            2162..>2711
                     /locus_tag="VFA35_05675"
     CDS             2162..>2711
                     /locus_tag="VFA35_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZT55697.1"
                     /translation="MEFGLATYLLGYLAGALSTLSPCVLPLLPILIATAVSQHRFGPL
                     ALAAGLALSFAAVGVFIATVGVAVGLDQGVLRSVAALLLVLFGVLMMLPWLQQRLLAL
                     AAGLSAPGWMSQLSGHGWRGQLAVGLVLGIVWAPCVGPTLGAASVLAAQGSHLPQVLL
                     LMLLFGLGAGTPLVLVGKLSREL"
BASE COUNT          378 a          938 c          956 g          439 t
ORIGIN      
        1 tgaacccgac cgagacgatt cgcgaggccc tgccggccga tcccgccgcg ctgcgcacgg
       61 cggcacgcag cgtctaccgc caccgcctgc cggtgcgcgt gatgcactgg atcaacgtga
      121 tctgcctgac gatcctgctg atgagcgggc tgcagatctt caacgcgcac ccggcgctgt
      181 actggggcaa ggattcgagc ttcgcgcagc cctgggtcgc gatcggcgcg ctgaacgcgc
      241 ccggcggcgg cgtcaccggc gttacgacga tcgccggcca ccgcttctcg accgacggcg
      301 tgctcggcgc gtccaaggtc ggcggcgagg gcgccaaggt ggtccgcgcg ttcccgtcgt
      361 gggccacgat ccccggcccg caatggctct cgatgggccg gcactggcat ttcttcttcg
      421 cgtggctgtt cgtgctgaac ggcatcgcct acgtggccta cgcgatcgcc agcggccacc
      481 tgacgcgcga cctgctgccc acgcgtgccg agctgcgcca gatcggccgc tcgatcaagg
      541 accacctgct gttcaggcac gaaaccggcg cggccgcgac gcgctacaac gtgctgcaga
      601 acctcgccta ccttgcggtg atcttcgggc tgctgccgct ggtgatcgtc gcgggcatgg
      661 cgatgtcgcc gcggctcgac gcggtggccg ccggctgggt cgacctgctc ggcggccggc
      721 agtcggcgcg cacgctgcac tttctggccg cgaccgggct gctgctgttc gtgttggtgc
      781 acctgttcga ggtgctgatc aacggggcat ggaaccagat ccgctcgatg ctgaccggct
      841 ggtacgtgct gccggtggac gacaagagga cgaagccatg atccgcaaac cgatctcgag
      901 cggccagccg aaggccgcgg ccagccgccg cacggccttg cgcgcggcgc tcgcgggcct
      961 gggcgcggcc tcgctcgcgg gctgcgaccg cttgtcgaac aacgccgcgt tcgtcgacgt
     1021 gctgaagagc acgcagcacc tcagccacgg cgcgcagcgg cttgtcgccg gccgcaaggc
     1081 gatggcgcag gagttcagcc aggccgacgt cgccgcgcac ttcaaggcca acggcacgct
     1141 cgcgccgcgc gacgccgact accaggcctt gatgaaggac ggtttcgccg actggaagct
     1201 gcaggtcggc ggcctggtcg acgcgcccgc tgcgttcacg ctcgccgagc tgcgcgcgat
     1261 gccgtcgcgc acgcagatca cgcgacacga ctgcgtcgag ggctggagca cgatcggcaa
     1321 gtggaagggc gtgccgctga gccatctgct ggcgctggtc aagccgcgcc cgcaagcgaa
     1381 attcgtggtg ttccgctgcg ccgactcgat ggaagatccg aacgcgagcg ccgactcccg
     1441 gtactacgaa agcgtcgaca tggacgatgc gtaccacgtg cagaccgtgc tcgcctacga
     1501 gctcaacgac gcgccgctgc cggttgccaa cggcgcgccg ctgctcgcgc gcatcgagcg
     1561 ccagctcggc tacaagcatg ccaagtacct gatgcgcgtc gagctggtcg agcgcttcga
     1621 ccagatccgc ggcgggcagg gcggctactg ggaagacaac ggctacgagt ggtatgccgg
     1681 catctgaggc gggggcatcc gcgccgcgct aagctcgcgg ctccccgttg aggagcgcgc
     1741 atgcgcaaac tgtttcgctc attcctggtg ttcatcgccc tcggcttcgc ggcgctcgcc
     1801 gctggcgccg ccgaactgcc cttcgacaag gcgaagctcg acgccgcgct cgcgcagcgc
     1861 aagccggtca tcgtgcactt cgcggccgac tggtgcccga cctgcaagga gcagaagccg
     1921 atcgtcgcgg cgctgatgca ggagcccaaa cgccaggata tcgcgctgtt cgtcgccgac
     1981 ttcgacaagg aaacggcgct gaagaagcgc ctgaaggtca cgatgcaaag caccttcgtc
     2041 gtgttcaagg gcggcaagga agtgggccgc tcgaccgggc agaccgaccc cgccgagctc
     2101 gccgccgtct tcgacaaggc gctctgagct tcgcgcgccg gcaagcacgg ggcggctggc
     2161 cgtggaattc gggcttgcga cctatttgct cggctacctg gctggggcgc tctcgaccct
     2221 gtcgccctgc gtgctgccgc tgttgccgat tctgatcgcg accgcggtgt cgcagcaccg
     2281 cttcggcccg ctcgcgctcg cggcggggct ggcgttgtcg ttcgccgcag tcggcgtgtt
     2341 catcgcgacc gtcggcgtcg cggtcggtct ggaccagggc gtgctgcgca gcgtcgccgc
     2401 gctgctgctg gtgctcttcg gcgtgctgat gatgctgccg tggctgcagc agcgcctgct
     2461 cgcgctcgcc gctggcctga gcgcgcccgg ctggatgagc cagctctcgg gccacggctg
     2521 gcgcgggcag ctggcggtcg ggctggtgct gggcatcgtc tgggcgccgt gtgtcggccc
     2581 gacgctcggt gccgcgagcg tgctcgccgc acaaggcagt cacctgccgc aggtgttgct
     2641 gctgatgctg ctgttcgggc tgggcgccgg cacgccgctg gtgctggtgg gcaagctgtc
     2701 gcgcgagttg a
//
LOCUS       DASRDG010000341         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1085138, whole genome shotgun sequence.
ACCESSION   DASRDG010000341 DASRDG010000000
VERSION     DASRDG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085138"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFA35_05680"
     CDS             <1..853
                     /locus_tag="VFA35_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZT55698.1"
                     /translation="LFKQLEAVRWNMDKDIPWDHFDASKLTEEQAQTIKMNAITEWAA
                     LPATEMFLRDNKDDADFCGFMSVWFFEEQKHSLVLMEYLRKFRPDLVPTEAELCEVRF
                     EFDPAPALETLMLHFCGEIRLNHWYRRAAEWHTEPVIQAIYETLARDEARHGGAYLRY
                     MKRALQNFGDEARAAFAKVGVLMASARRTAQALHPTNLHVNVKLFPRDTVQSRLPNPD
                     WLEKWLDKQIQFDAVWETRVVERILHNMSLLMERSFASVQELNRFRKEVTARLAAAGQ
                     PASAAPA"
     gene            874..1347
                     /gene="rfaE2"
                     /locus_tag="VFA35_05685"
     CDS             874..1347
                     /gene="rfaE2"
                     /locus_tag="VFA35_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HZT55699.1"
                     /translation="MPDFLDKLCPRAELAARVAALPRPVVFTNGVFDILHRGHVSYLA
                     QARALGASLVLGLNSDASARLLGKGPDRPLNAELDRACVLAALQSVSLVTLFDEATPV
                     ELLKLVRPQLYVKGGDYDIETLEETKWVRSWGGDACALSFVDGYSTSALVRRIRA"
     gene            complement(1364..>2044)
                     /gene="rrtA"
                     /locus_tag="VFA35_05690"
     CDS             complement(1364..>2044)
                     /gene="rrtA"
                     /locus_tag="VFA35_05690"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HZT55700.1"
                     /translation="ARRGARAVARRVRRARACGVMHVPAGGRAWLGVAALLAAGALAG
                     WPVAHEAIDWQPGLASTQPWRAFTAIGVHYSALHLAANLAGTALVGCFGAVARVPRRC
                     AWAWLAAWPLTQFGLLLKPELLHYGGLSGVLHAGVAIVGIYLLATGARAQRGTGARAQ
                     GALAAAVLVGLVAKLLSEAPWGDALRHPAGWDIAVAPLAHATGALAGVITGAIGAAIA
                     LRLPRRAA"
BASE COUNT          324 a          753 c          709 g          258 t
ORIGIN      
        1 acttttcaag cagctcgaag cggtgcgctg gaacatggac aaggacatcc cttgggacca
       61 tttcgacgcc agcaagctca ccgaagagca ggcccagacg atcaagatga acgcgatcac
      121 cgagtgggcg gcgctgccgg cgaccgagat gtttctgcgc gacaacaagg acgacgccga
      181 cttctgcggc ttcatgagcg tctggttctt cgaggaacag aagcactcgc tggtcctgat
      241 ggaatacctg cgcaagttcc gccccgacct cgtgccgacc gaagccgagc tgtgcgaggt
      301 gcgcttcgag ttcgacccgg cgccagcgct cgagacgctg atgctgcact tctgcggcga
      361 gatccgcctg aaccactggt accggcgtgc cgccgaatgg cacaccgaac cggtgatcca
      421 ggcaatttac gagaccctgg cgcgcgacga agcccgccac ggcggcgcct acctgcgcta
      481 catgaagcgc gcgctgcaga acttcggcga cgaggcgcgc gccgcgttcg cgaaggtcgg
      541 cgtgctgatg gccagcgcgc gccgcaccgc gcaggcgctg catccgacca acctgcacgt
      601 caacgtcaag ctgttcccgc gcgacaccgt gcaaagccgg ctgccgaacc ccgactggct
      661 cgagaagtgg ctcgacaagc agatccagtt cgatgccgtc tgggagaccc gggtcgtcga
      721 gcgcatcctg cacaacatga gcctgctgat ggagcgcagc ttcgccagcg tccaggagct
      781 gaaccgcttc cgcaaggaag tgacggcccg gctcgccgcc gccggccagc cggcgagcgc
      841 ggcgccggcc tgatcggcgc acccccgccg gtcatgcccg atttcctcga caagctctgc
      901 ccccgtgccg agttggcagc gcgcgtggcc gcactgccgc ggccggtcgt gttcacgaac
      961 ggcgtgttcg acatcctgca ccgcggccac gtcagctacc tcgcgcaagc gcgcgcgctc
     1021 ggcgccagcc tggtgctcgg gctgaacagc gacgcgtcgg cgcggctgct cggcaagggg
     1081 ccggatcgcc cgctgaacgc cgagctcgac cgcgcctgcg tgctcgccgc gctgcagagc
     1141 gtcagcctcg tgacgctgtt cgatgaggcc acgccggtcg agctgctgaa gctggtgcga
     1201 ccgcagctct acgtgaaggg cggcgactac gacatcgaga cgctcgaaga aaccaagtgg
     1261 gtgcgcagct ggggcggcga cgcgtgcgcc ctgtcgttcg tcgacggcta ctcgacgagc
     1321 gcgctggtgc gccgcatccg cgcctgacgc gcggtcgctc ggctcacgcc gctcgacgcg
     1381 gcaaccgcag cgcgatcgcc gcgccgatcg cgccggtgat cacgcccgcc agcgcgccgg
     1441 tcgcgtgggc gagcggcgcg accgcgatgt cccagcccgc agggtggcgc agcgcgtcgc
     1501 cccagggcgc ttcgctcagc agcttggcga cgagcccgac caggaccgcc gcggccagcg
     1561 cgccctgcgc gcgtgcgccg gtcccgcgct gcgcgcgtgc gccggtcgcg agcaggtaga
     1621 tgccgacgat cgcgacgccc gcgtgcagca cgcccgacag gccgccgtag tgcagcagtt
     1681 cgggcttcag cagcaggccg aactgggtca gcggccaggc ggcgagccag gcccacgcgc
     1741 agcggcgcgg cacccgcgcg accgcgccga agcacccgac cagcgcggtg ccggcgaggt
     1801 tcgcggccag gtgcagcgcg ctgtagtgca cgccgatcgc ggtgaacgcg cgccacggct
     1861 gcgtggaggc gagcccgggt tgccagtcga tcgcctcgtg cgcgaccggc cagcccgcca
     1921 gcgcgcccgc cgccagcagc gccgccacgc cgagccatgc gcggccgccg gccggcacgt
     1981 gcatcacccc gcaggcgcgg gcgcgtcgaa cacgacgtgc cacagcgcga gcaccgcgtc
     2041 gcgc
//
LOCUS       DASRDG010000342         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_624163, whole genome shotgun sequence.
ACCESSION   DASRDG010000342 DASRDG010000000
VERSION     DASRDG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624163"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFA35_05695"
     CDS             complement(<1..256)
                     /locus_tag="VFA35_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55701.1"
                     /translation="MVPEEQQLEAGIAALESQRATLGDTVVEAALEGLKARLAALRKT
                     PAPEPEPAQSLKQVSILFLDVVGSTALGQRLDPEEISAVME"
     gene            complement(268..1929)
                     /locus_tag="VFA35_05700"
     CDS             complement(268..1929)
                     /locus_tag="VFA35_05700"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HZT55702.1"
                     /translation="MALAQLEQHLEIRRFEPGDIVLRAGVESAELFGLIEGAVRIELG
                     DGGRRLQLVAPQCFGELSALTGDPVSATVIAHRDVRAWVLSAPVLFEAMAQETAFFRN
                     VATLLGMRLRERTRRAPTRRPRVALLPLRDEGDRLLLAALGRGLHHYAPGSEHDAVPG
                     DDVAGGAGRMRRWRDEGHGDAVLLLGVGLAGCAALLCDLDADDVLLVAEDDLPGLPHA
                     LAQPIVWRRGPLSAAAALQRWSHALPREEIEAGASGNDWSRARQPALDHLVRRLCGRD
                     VGVAMSVGAAAGLAHLGVLEVIEEDGLPIDFLCGSSMGGAVALAFARFGSAHAAGEAI
                     LGHVADFARSKGIQWLPRASLISASRMTTLTEELFGNATFAQLRLPIAVVAADLAMGK
                     RAVLDSGPVAPAARATAAIPGVFAPVRLGDALLVDGAVVSRLPVDLLVARGCGFKLAA
                     LVRPTYPQEAPGAARAADRLEQRLHRPFGLRAAMGGAYRLQGWWDSASHAGRADLSLT
                     VPIPVGEGFNFAMAEAMVECGRRTARERLPQIRLALQQVLAPGVP"
     gene            complement(1974..>2560)
                     /locus_tag="VFA35_05705"
     CDS             complement(1974..>2560)
                     /locus_tag="VFA35_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HZT55703.1"
                     /translation="YTMKRQALWALTELPGAASLREPATAVARHVRPEVAWRNHEASL
                     DAAQLEPLSRTKLTYVLDEFFIPPRRFEPFVREMGAILRRHHVAAVNVSIRHSPADAL
                     ALLPWAREEVFSFVLFYRQGLDLAAQRAVGEWTRELIDATLRHEGRYYLPYQLHASRE
                     QFERAYPEVAQLRQLKSRVDPQGRFTNSLWQKYL"
BASE COUNT          365 a          927 c          953 g          315 t
ORIGIN      
        1 cttccatcac cgcgctgatt tcctccgggt cgaggcgctg gccgagcgcg gtggagccga
       61 ccacgtcgag aaagaggatg ctgacctgct tgaggctttg cgcgggctcg ggttcgggcg
      121 cgggcgtctt gcgcaacgcg gccagccgtg ccttcagccc ctcgagcgcc gcctcgacca
      181 cggtatcgcc gagcgtggcc ctttgacttt caagtgcggc aatgcctgcc tcgagttgct
      241 gctcctccgg caccatgggc ccacctctca cggcactcct ggagcaagga cctgctgcag
      301 cgccaggcgg atctgcggca ggcgctcgcg ggccgtgcgc cggccgcact cgaccatcgc
      361 ctcggccatg gcgaagttga agccttcgcc caccggaatc ggcacggtca gcgacaggtc
      421 ggcgcggccg gcgtggctgg ccgagtccca ccagccctgc aggcggtagg cgccgcccat
      481 cgcggcgcgc aggccgaacg gccgatgcaa gcgctgctcg aggcgatcgg cggcgcgcgc
      541 ggcgccgggt gcctcctgcg gatacgtcgg ccggaccagc gccgcgagct tgaagccgca
      601 gccgcgcgcg accagcaggt ccaccggcag gcgcgacacg acggcgccgt cgacgagcaa
      661 ggcgtcgccc agccggaccg gggcgaatac gccgggaatg gcggccgtcg cccgtgcggc
      721 cggcgccacc gggccgctgt cgagcacggc gcgcttgccc atcgccaggt cggcagcgac
      781 caccgcgatc ggcaggcgca gctgggcgaa ggtggcgttg ccgaacagct cctcggtgag
      841 cgtcgtcatg cgcgaggcgg agatgagcga ggcccgcggc agccactgga tgcccttgga
      901 gcgcgcgaag tccgcgacgt ggccgaggat cgcctcgccc gctgcgtggg cgctgccgaa
      961 tcgcgcaaag gccagggcca cggccccgcc catgctcgag ccgcagagaa agtcgatcgg
     1021 caggccgtcc tcttcgatca cctcgagcac gccgagatgg gccaggccgg ccgccgcccc
     1081 gacgctcatg gccacgccga cgtcccggcc gcagagccgc cgcacgaggt ggtccagcgc
     1141 cggctggcgc gcgcgcgacc agtcgttgcc cgaggcgccg gcctcgatct cttcgcgcgg
     1201 cagggcatgc gaccagcgct gcaaggctgc cgccgcggag agcggcccgc gccgccagac
     1261 gatgggttgg gcgagcgcgt gcggcaggcc gggcaggtcg tcttcggcga cgagcagcac
     1321 gtcgtcggca tcgaggtcgc agagcagggc cgcgcagccc gccagaccga cgccgagcag
     1381 cagcaccgcg tcgccgtgcc cttcgtcgcg ccagcggcgc atgcgcccgg cgccgcccgc
     1441 gacgtcgtcg cccggcaccg cgtcgtgctc cgagccgggc gcgtagtggt gcaggccccg
     1501 gcccagcgcg gccagcagga gccggtcgcc ctcgtcgcgc agcggcagca gcgcgacccg
     1561 cggtcgcctc gtcggcgcgc gccgcgtgcg ctcgcgcagg cgcatgccca ggagggtggc
     1621 gacgttgcgg aagaaggcgg tctcctgcgc catcgcctcg aacaggaccg gcgccgagag
     1681 cacccaggcc cgcacgtcgc gatgggcgat cacggtggcc gagaccggat cgccggtcag
     1741 cgcagacagc tcgccgaagc attgcggcgc caccagttgc agccggcggc cgccgtcgcc
     1801 gagctcgatc cgcaccgcgc cctcgatcag cccgaacagc tcggccgact cgacgccggc
     1861 gcgcaggacg atgtcgcccg gctcgaaccg gcgtatctcc aggtgctgct cgagctgcgc
     1921 gagcgccatc gccgacacgt tggcgaagag cggatgcagg gccagcgagg gtgtcagagg
     1981 tatttctgcc aaagcgagtt ggtgaagcgt ccctgcggat cgacgcgcga cttgagctgc
     2041 cgcagctgcg cgacttcggg ataggcgcgc tcgaactgct cgcgcgaggc gtgcagctgg
     2101 tacggcaggt agtagcgccc ctcgtgccgc agcgtcgcgt cgatgagctc gcgcgtccat
     2161 tcgccgacgg cccgctgggc cgcgaggtcg aggccctggc ggtagaagag aacgaacgag
     2221 aacacctctt cgcgggccca gggcaggagc gccagtgcat ccgccgggct gtgccggatc
     2281 gagacgttga ccgcggcgac gtggtggcgg cgcaggatcg cgcccatctc gcgcacgaag
     2341 ggctcgaagc gccgcggcgg gatgaagaac tcgtcgagca cgtaggtcag cttggtgcgc
     2401 gagagcggct cgagctgggc cgcgtcgagg ctcgcctcat ggttgcgcca ggccacttcg
     2461 gggcggacgt ggcgcgcgac ggcggtcgcc ggctcgcgca ggctcgcggc cccgggcagc
     2521 tcggtcagcg cccacagcgc ctggcgcttc atcgtgtagg
//
LOCUS       DASRDG010000343         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1658430, whole genome shotgun sequence.
ACCESSION   DASRDG010000343 DASRDG010000000
VERSION     DASRDG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658430"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFA35_05710"
     CDS             complement(<1..813)
                     /locus_tag="VFA35_05710"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HZT55704.1"
                     /translation="MTPGRREQLRAMFAQALQKLAVGPAVRHKVARHRPDLADARSVV
                     LLAFGKAARPMAQAALEELDGLPVRGLCAPPEPDGAPLPPLEVLPGGHPLPSAGSLRA
                     GARALELCRTAAADDRIVFLVSGGGSSILELPLDPAVPLGELRALYQALLGCGAPIAA
                     VNTVRKHFSAVKGGRLAAAAAHTPLLMTLAVSDVPDGQPAALASGPTLGDSTTTADCR
                     ALLQRFSLLDAVPKVLRERLDRGLLPPPVPADHPVFARSEFCMLLDNSHARFH"
     gene            complement(810..1772)
                     /locus_tag="VFA35_05715"
     CDS             complement(810..1772)
                     /locus_tag="VFA35_05715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZT55705.1"
                     /translation="MLVTRRVYPAALAILQEDCVVDYRDTLDVMDEALLARRLQHCDG
                     VVCQLTDPMTAGVFAAAPKLRVAAQIAVGHDNVDVAAATARGIVVTNTPGVLTESTAD
                     LAFALLLATARRLPAAERFLRDGNWRRWDIDLLAGTDVHGRTLGIVGFGRIGQAVARR
                     ARGFGMRVLCSTRTPPLPALLQELAAQHVPLDFLLAHSDFVSLHAPIRWCVSGPSGVE
                     QLARMRRGAILVNTARGPLVDERALAAALAEGLLGGAGLDVFEDEPRVHAELLRLPNV
                     VLLPHVGSATVATRTRMCTLAAENCTAVLRGQRPPNAVNPEALQ"
     gene            2018..>2604
                     /locus_tag="VFA35_05720"
     CDS             2018..>2604
                     /locus_tag="VFA35_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55706.1"
                     /translation="MPLPRVSAFAALVSLAGGASAQAAADPRLHTVFGAVDPLDGLAD
                     VPPALAAPADGRLFVVQFATAIQPAYARALAARRLSVVAYLPANAYVVRGARADAEAF
                     RALPPAGSPVRWVGPLPLAWKLAKALQPLAMAPAAGGDAIVCNLLLADKADEAPLCAQ
                     IRSLGGEVVVHGPVLVRARLRPLPLRAVAALDGVL"
BASE COUNT          303 a          962 c         1020 g          319 t
ORIGIN      
        1 gtggaagcgg gcgtggctgt tgtcgagcag catgcagaac tcgctgcgcg cgaacaccgg
       61 gtggtcggct ggcacgggcg gcggcagcag accccggtcg agccgttcgc gcaggacctt
      121 cgggacggcg tcgagcagcg agaagcgctg cagcagtgcg cggcagtcgg cggtcgtcgt
      181 cgagtcgccg agcgtcggcc ccgatgccag cgccgcgggc tgtccgtcgg gcacgtccga
      241 caccgcgagc gtcatcagca ggggcgtgtg cgcggcggcg gcggcgagcc ggccgccctt
      301 cacggcggag aagtgcttgc gcaccgtgtt gacggccgcg atcggcgcgc cgcagccgag
      361 cagcgcctgg tagagcgcgc gcagttcgcc gagcggcacg gcgggatcga gcggcagttc
      421 gaggatcgag ctgccgccgc cggagaccag gaacacgatg cggtcgtcgg cggccgcggt
      481 gcggcacagt tcgagcgcgc gcgcgccggc gcgcaggctg cccgcgctgg gcagcggatg
      541 gccgcccggc agcacttcga gcggcggcag aggcgcgccg tcgggttcgg gcggcgcgca
      601 gagcccgcgc accggcaggc cgtcgagctc ctccagcgcc gcctgcgcca tcggccgcgc
      661 cgccttgccg aacgcgagca gcaccaccga gcgcgcgtcg gcgaggtccg gccggtgccg
      721 cgccaccttg tggcgcacgg cgggaccgac ggcgagcttc tgcagcgcct gcgcgaacat
      781 cgcccgcagc tgctcgcggc ggcccggcgt cactgcagcg cctcggggtt gacggcgttg
      841 gggggacgct ggccgcgcag cacggcggtg cagttctcgg ccgcgagcgt gcacatgcgc
      901 gtgcgcgtcg cgacggtggc gctgccgacg tgcggcagca gcacgacgtt cggcagccgc
      961 agcagctctg cgtgcacgcg cggttcgtcc tcgaacacgt cgaggccggc gccgccgagc
     1021 agaccctccg cgagcgcggc ggcgagcgcg cgttcgtcga cgagggggcc gcgtgcggtg
     1081 ttgacgagga tggcgccgcg gcgcatgcgc gcgagctgct cgacgccgct cggccccgac
     1141 acgcaccacc tgatcggcgc gtgcagcgac acgaagtcgc tgtgcgcgag caggaagtcg
     1201 agcggcacgt gctgggccgc cagttcctgc agcagcgcgg gcaggggcgg cgtgcgcgtg
     1261 ctgcacagca cgcgcatccc gaagccgcgc gcgcgccgtg cgaccgcctg gccgatgcgg
     1321 ccgaagccca cgatgccgag cgtgcggccg tgcacgtcgg tgccggcgag caggtcgatg
     1381 tcccagcgcc gccagttgcc gtcgcgcagg aagcgctcgg ccgccggcag ccggcgcgcg
     1441 gtggcgagca gcagcgcgaa cgcgaggtcg gcggtcgact cggtcagcac gcccggcgtg
     1501 ttggtgacga cgatgccgcg cgcggtcgcg gcggcgacgt cgacgttgtc gtggccgacg
     1561 gcgatctgcg ccgcaacgcg cagcttcggc gcggctgcga acacgcccgc ggtcatcgga
     1621 tcggtgagct ggcagacgac gccgtcgcag tgctgcaggc ggcgcgcgag cagcgcctcg
     1681 tccatcacgt cgagcgtgtc gcggtagtcg acgacgcagt cctcctgcag gatcgcgagc
     1741 gcggcggggt agacgcgccg cgtgaccagc acgcgcggct tcacggacgc ccaggagacc
     1801 acgccggcgc ttccgtgcaa gggcaagatc gccgccggcg gcgaccgtcc gccgtacgcg
     1861 gtgacacggc ccagcgtctg gcggcccggc gtctggagca ccgccgcggg cggcttggcg
     1921 gcgaccggcg cgcgcgggac aatccatgcg ctcctccatc ccaccggccg cgccgcccgg
     1981 ccgttgcagt tccccggtcc caccgcatct ccaagtcatg ccgttgccgc gcgtctccgc
     2041 tttcgcggcc ctcgtgtcgc tcgccggcgg tgcgtccgcg caggccgccg ccgatccccg
     2101 tctgcacacc gtgttcggcg ccgtcgatcc gctcgacggc cttgcggacg tgccgccggc
     2161 gctcgcggcg ccggccgacg gccgcctgtt cgtcgtgcag ttcgcgaccg cgatccagcc
     2221 cgcgtacgcg cgcgcgctcg cggcgcggcg cctgtcggtg gtcgcctacc tgccggcgaa
     2281 cgcctacgtc gtgcgcggcg cccgcgccga cgccgaggcg ttccgtgcgc tgccgccagc
     2341 gggcagcccg gtgcgctggg tcgggccgtt gccgctcgcg tggaagctcg ccaaggcgct
     2401 gcagccgctc gccatggcgc cggcggccgg cggcgacgcg atcgtgtgca acctgctgct
     2461 cgccgacaag gcggacgaag cgccgctctg cgcgcagatc cggtcgctcg gcggcgaggt
     2521 cgtcgtgcac gggccggtgc tcgtgcgcgc gcgcctgcgg ccgctgccgc tgcgtgcggt
     2581 cgcggctctc gacggggtgc tctg
//
LOCUS       DASRDG010000344         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_857300, whole genome shotgun sequence.
ACCESSION   DASRDG010000344 DASRDG010000000
VERSION     DASRDG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857300"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VFA35_05725"
     CDS             <1..1258
                     /locus_tag="VFA35_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55707.1"
                     /translation="MIAKLDPTNPKPAGVSGGLTSVNVATRTFVLEKRALDNTSICSF
                     TVAAASNTVFQIDGTVYAGFGGLATLANQSLGAAHVFVQGTYGTGAHTINALAVESGS
                     GVPGNGQSWVVGHVVGRDHTAGQDATLSVLGHSFDVGSGTRLYNTLHTVQVALGTTKV
                     LRFGAGNSLTTDALQVGQRVAVFGTLTGTNLDARGIGTTPDVARILFTSVFGIATGAP
                     VGNTLTIDVARFDLRDISNFNFSVGGITESDPHTYTVDVTGLSTTGITTNSRVRCIGW
                     ANPVGVAGDSDFQSISLVDRGVDGKAMLCFWSPANAQALPSVSSTAIGLDVSAAAVKV
                     VSDGFTTTGINTSPAPSLEPFTSIGFYLIVQNSGIELHLQFAPFTTSLSNRLLAGAAV
                     RRVSALGRYDAPSQTFAALVSTVVLQ"
     gene            1370..2005
                     /locus_tag="VFA35_05730"
     CDS             1370..2005
                     /locus_tag="VFA35_05730"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HZT55708.1"
                     /translation="MPPIPAPPQRPWLFPALTALCALLAFWSFLGAADRAVWAFEVLP
                     LGAVMVAFAVRRRWFHFTDLAYALLAWFFAIQCIGGRYTFEHVPFPQALTEWFGRNPA
                     DRCGHFFQGFVPAILLREWLRRRRGVAAGYTLFWLVTGCCLGMSAAYELLEMAVVLLC
                     YPKDGPQWLGMQGDPFDAQWDMTTALGGAIAAQLLLRRLHERSIARADAGR"
BASE COUNT          285 a          798 c          708 g          327 t
ORIGIN      
        1 gatgatcgcc aagctcgatc cgaccaatcc gaagccggcg ggcgtctccg gcgggctgac
       61 gtcggtgaac gtcgccaccc gcacgttcgt gctcgagaag cgcgcgctcg acaacacgtc
      121 gatctgctcg ttcaccgtcg ccgccgccag caacacggtg ttccagatcg acggcaccgt
      181 ctacgcgggc ttcggcggcc tcgccacgct cgccaaccag agcctcggcg ccgcgcacgt
      241 cttcgtgcag ggcacgtacg gcaccggcgc gcacacgatc aacgcgctcg ccgtcgagtc
      301 cggctccggc gtgccgggca acggccagag ctgggtcgtc ggccacgtcg tcggccgcga
      361 ccacacggcg ggccaggacg cgacgctgtc ggtgctcggc cactcgttcg acgtcggcag
      421 cggcacgcgc ctctacaaca cgctgcacac cgtgcaggtg gcgctaggca ccacgaaggt
      481 cctgcgcttc ggcgccggca actcgctgac caccgacgcg ctgcaggtcg gccagcgcgt
      541 ggccgtgttc ggcaccctga ccggcaccaa cctcgacgca cgcggcatcg gcacgactcc
      601 ggacgtcgcg cgcatcctgt tcaccagcgt cttcggcatc gccaccggcg caccggtcgg
      661 gaacacgctc acgatcgacg tcgcccgctt cgacctgcgc gacatctcga acttcaactt
      721 cagcgtgggc ggcatcaccg agtccgatcc gcacacgtac acggtcgacg tgaccggcct
      781 gtcgacgacg ggcatcacca ccaactcgcg cgtccgctgc atcggctggg ccaacccggt
      841 cggcgtcgcc ggcgacagcg acttccagtc catcagcctg gtcgaccgcg gcgtcgacgg
      901 caaggcgatg ctctgcttct ggtcgccggc gaacgcgcag gcgctgccga gcgtcagcag
      961 cacggcgatc gggctcgacg tcagcgccgc ggccgtgaag gtcgtgagcg acggcttcac
     1021 gaccacgggc atcaacacct cgccggcgcc gagcctcgag ccgttcacga gcatcggctt
     1081 ctacctgatc gtccagaaca gcggcatcga gctgcacctg cagttcgcgc cgttcaccac
     1141 ctcgctgtcg aaccgcctgc tcgccggcgc ggccgtccgc cgcgtctccg cgctgggccg
     1201 ctacgacgcc ccgtcgcaga cgttcgcggc gctcgtctcg acggtcgtgc tgcaatagcc
     1261 gcgttgcaat cgcggcgcgc gacttcgcga cgcgggccgg gaacgacgat gcggcggcgg
     1321 cggtgccacg cgcgtgttct gccggagtgc cggcgcctat cgtccgcgca tgccgccgat
     1381 ccccgcgccg ccgcagcgcc cctggctgtt cccggccctg accgcgctct gcgcgctgct
     1441 cgcgttctgg tcgttcctgg gcgccgcgga ccgggcggtg tgggcgttcg aagtgctgcc
     1501 gctgggcgcc gtgatggtcg cgttcgccgt gcggcgccgc tggttccact tcaccgacct
     1561 cgcgtatgcg ctgctcgcgt ggttcttcgc gatccagtgc atcggcggcc gctacacgtt
     1621 cgagcatgtg ccgttcccgc aggcgctgac cgagtggttc ggccgcaacc ctgccgaccg
     1681 ctgcggccac ttcttccagg gcttcgtgcc ggccatcctg ctgcgcgaat ggctgcggcg
     1741 ccgccgcggc gtcgcggccg gttacacgct gttctggctc gtcacgggtt gctgcctcgg
     1801 catgagcgcc gcctacgaac tgctcgagat ggcggtagtg ctgctgtgct acccgaagga
     1861 cgggccgcag tggctcggca tgcagggcga cccgttcgac gcgcagtggg acatgacgac
     1921 ggcgctcggc ggcgcgatcg cggcgcagct gctgctgcgg cggctgcacg agcgcagcat
     1981 cgcgcgcgcc gacgccgggc gttgaaccga gtccggcccg ccctcggcgc gccctgagcc
     2041 gaaccgcctc gggcgtgttc ggctcagaga ccgagaaaca atcctgactc gagcccgcct
     2101 tcggcgtgct cgagtcag
//
LOCUS       DASRDG010000345         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_413229, whole genome shotgun sequence.
ACCESSION   DASRDG010000345 DASRDG010000000
VERSION     DASRDG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413229"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFA35_05735"
     CDS             complement(<1..492)
                     /locus_tag="VFA35_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT55709.1"
                     /translation="MRIRIALITALALCATLAAAQQDEGDALGYATRAKAPPGYPASY
                     QAMVRAAEDEGRVIVYSTTDEAVARPLIADFRALYPRIEVQYEDLNSTELHYRFIAES
                     QLGGETADVLWSSAMDQQASLISRGYAATYESPESAGLPAWAQWKGQAYASTYEPVVI
                     AYDK"
     gene            complement(512..1828)
                     /locus_tag="VFA35_05740"
     CDS             complement(512..1828)
                     /locus_tag="VFA35_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HZT55710.1"
                     /translation="MLTLLAYSMVVVFMALIMTKRLPAVVALIVIPILFGLFGGFGIS
                     LGPMMMDGIKKLAPTGVMLMFAILYFGIMIDAGLFDPAVRVILRLVGGDPMKVVVGTA
                     VLAMIVGLDGDGSTTYMVTGAAMLPLYFRLGISRLVFACVVMMSSHNMNLLPWGGPTA
                     RATSALSLEMSDVFVPLIPAMVITAAWVLFVAYILGMRERKRLGIVSLGDVDPEHPEA
                     TLVGNDPSLLRPKLLWLNFALTVGLIATLIWGKFPLPVLFMVAFAIAISVNYPNLQIQ
                     KERIAHHAENVLPVVALIFAAGIFVGILSGTKMVDAIADSVIRGVPTWMGPYMGLVTA
                     ALSLPFTFFISNDAFYFGIIPILAKTAAVYGFTPAEIARASLIGQQVHLLSPLVASTY
                     LLVGLAKIEFGDHQRYTLLWAISAAAVMTLAVFGLGVVPLVRHAAG"
BASE COUNT          351 a          689 c          676 g          315 t
ORIGIN      
        1 cttgtcgtag gcgatcacca ccggctcgta ggtgctcgcg taggcctggc ctttccactg
       61 ggcccaggcc ggcaggccgg cgctttccgg cgattcgtag gtggccgcgt agccgcggct
      121 gatcaggctg gcctgctgat ccatcgccga gctccacagc acgtcggcgg tctcgccgcc
      181 gagctgcgat tcggcgatga agcggtagtg cagctcggtg ctgttcaggt cttcgtactg
      241 cacctcgatg cgcgggtaca gcgcgcggaa gtccgcgatc agcgggcgcg cgacggcctc
      301 gtcggtggtc gagtagacga tcacgcggcc ctcgtcctcg gcggcacgca ccatcgcctg
      361 gtagctcgcc gggtagcccg gcggcgcctt cgcgcgcgtc gcgtagccga gcgcgtcgcc
      421 ttcgtcctgc tgcgccgcgg cgagcgtggc gcacagcgcg agcgcggtga tcagtgcgat
      481 tcggatgcgc atggtcgctg ctcccgggag ttcagccggc ggcgtgccgc accagcggca
      541 cgacgcccag gccgaagacc gcgagcgtca tcaccgctgc cgccgagatc gcccacagca
      601 gcgtgtagcg ctggtgatcg ccgaactcga tcttggccag gccgaccagc aggtaggtcg
      661 aggccaccag cgggctcagc aggtgcacct gctggccgat cagcgaggcg cgcgcgatct
      721 cggccggcgt gaagccgtag accgcggccg tcttcgcgag gatcgggatg atgccgaaat
      781 agaacgcgtc gttcgagatg aagaacgtga acggcaggct cagcgccgcg gtgaccaggc
      841 ccatgtacgg gcccatccag gtcggcacgc cgcggatcac gctgtcggcg atcgcatcga
      901 ccatcttggt gcccgacagg atgccgacga agatgccggc cgcgaagatc agcgccacca
      961 ccggcagcac gttctcggcg tggtgcgcga tgcgctcctt ctggatctgc aggttcgggt
     1021 agttcacgct gatcgcgatc gcgaacgcca ccatgaacag caccggcaac gggaacttgc
     1081 cccagatcag cgtcgcgatc aggcccaccg tcagcgcaaa gttcagccac agcagcttcg
     1141 ggcgcagcag cgacgggtcg ttgcccacca gcgtggcctc ggggtgctcc gggtcgacgt
     1201 cacccagcga cacgatgccc aggcgcttgc gctcgcgcat gcccaggatg taggcgacga
     1261 acaggaccca ggcggcggtg atcaccatcg ccgggatcag cggcacgaac acgtcggaca
     1321 tctccagcga cagcgcgctg gtggcacgcg ccgtggggcc gccccagggc agcaggttca
     1381 tgttgtggct ggacatcatc acgacgcagg cgaacacgag ccggctgatg ccgaggcgga
     1441 aatagagcgg cagcatcgcc gcgccggtga ccatgtaggt ggtcgatcca tcgccgtcca
     1501 gcccgacgat catcgccagc accgcggtcc ccaccaccac cttcatcggg tcgccgccca
     1561 ccagccgcag gatcacgcgc acggccggat cgaacaggcc cgcgtcgatc atgatcccga
     1621 agtacaggat cgcgaacatc agcatcacgc cggtcggcgc gagcttcttg atgccgtcca
     1681 tcatcatcgg cccgagcgag atgccgaacc cgccgaacag cccgaacagg atcgggatga
     1741 cgatcagcgc gaccaccgcc ggcaggcgct tggtcatgat gagcgccatg aaaaccacga
     1801 ccatcgagta agccagcaac gtcaacattc ttgtctcctg ccgagtctgt tattcgcgtc
     1861 gcgacggcgc gtctgcatgc atgttattcc aagggctccc cgcagcgggt ccgctcgaag
     1921 catcacgtgc cggcccatct tgaggcgacc gggtcgcgtg gagctttcgc cgagctttca
     1981 gcgaagcgtt gggacttccc tcgatcgggt ttgtccgggt cgggtaagcg a
//
LOCUS       DASRDG010000346         6449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_624609, whole genome shotgun sequence.
ACCESSION   DASRDG010000346 DASRDG010000000
VERSION     DASRDG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624609"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFA35_05745"
     CDS             <1..877
                     /locus_tag="VFA35_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZT55711.1"
                     /translation="RTLFRGAGLDLADLREYQHFDDVRHIDWNVTARLQQPYVRQFTE
                     DRELTAWFLVDLSASVDFGSNERTKRGVAREFVGVLARLLTRRGNRIGALLYGTEVDT
                     VLPAGSGRRHVLELLQRMGRRPEQSAAGATDLRDLLVAAQGIVKHRGSVFVISDFISA
                     PGWERSLAQLATRHEVTAVRVLDPFEMALPDIGLVTMRDAESGEQLVVDTHDRGFRAR
                     FEAAAERRETALRGALARAGVDTLELATDDDLLDAILRFADLRKQRSRLANWSGQPGR
                     ASVGAHLSARSGVAA"
     gene            989..2041
                     /locus_tag="VFA35_05750"
     CDS             989..2041
                     /locus_tag="VFA35_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZT55712.1"
                     /translation="MTDWPSLPVTFFWPEMLWGLLAVPLLVLLYIWLLRRRKRSALRY
                     ASLSLVKEAIGQRVAWRRHVPPALLLTAITGLLIAAARPAAVITLPTAEKTVVLAIDV
                     SGSMRATDVQPDRMTAAKEAAKAFVADLPRSVRIAVVQFAGTAAVVQAPTLSREDVVA
                     AIDRFQLQRGTAIGSGIVLSLATLFPDAGIDLSQITGQREMPPAPGDKPRPAFTPVAP
                     GSYASAAIILLTDGQRTTGPDPVEAAKMAADRGVRVYTVGVGTKEGETIGFEGWSMRV
                     RLDEDTLKNIANLTHADYFYAGTANDLKQVYQNLSTRLVVEKKETEISGLFAAVGALL
                     VVVSAGLSVWWFGRVA"
     gene            complement(2042..3628)
                     /locus_tag="VFA35_05755"
     CDS             complement(2042..3628)
                     /locus_tag="VFA35_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HZT55713.1"
                     /translation="MTLWIALHLPLLSLESFAATLPHAAGERPLALLDAQHIVAVNAP
                     ARALGVQPGFKRATALALAPQLTFGLADPVRDAQALMAVAHAALAFTPTVVIEPPGDA
                     GSTTAATTAPGDTPHTVLLEVQASLRYFGGLAALQQRLTSALAPLGHHLHIVSASTPL
                     GAALLARVHPKLACADLAATTRALAHAPVWLLGPGRAQREALQGMGLHTLGDLQRVPR
                     DGLARRFGPAVLDALDRALGRRPDPRVSLALPPAFDSRLELFACADTTEQLMHGAAVL
                     LARLVAWLAAQHAFVRRFTLVMAHERRSRHDALGSGITSLEVALAEPSRDSAHLQVLL
                     RERFAQLQLPAPTLDLRLHADDIARRAPPNTELFPTPAAEHEGLTRLIERLQARLGRA
                     QVQRLVSVPDHRPERATACRAADAQMARARSPGGPAGAPEAAGAARPMRPVWLLQRPE
                     PLHERAVPGGERGPWADGQPLRLLSGPERIESGWWDFDVVARDYYIAQLPDGALVWIF
                     RERVPTSGVKLNWFLQGRFG"
     gene            complement(3654..4478)
                     /gene="imuA"
                     /locus_tag="VFA35_05760"
     CDS             complement(3654..4478)
                     /gene="imuA"
                     /locus_tag="VFA35_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HZT55714.1"
                     /translation="MLSVSAPPLPLLGMGGARQRPPPIRPERLHPALWLGHQLARHAD
                     VPIATGFEALDAQLPGAGWPRRALTELLLPHPGVGEIRLVAPSLVAAQVAGRLVMLFD
                     PPATLSASALAQLGIAVEELLIIDTHTRTRVVPGTDSLWALEQALKSGHVGAVLAWLP
                     PRLRAERLRRLQLAAHAHDGPAFVFCETSAAQRPTAAPLRLALRAHGPDVLAVRVLKR
                     RGPPLEAPLQLALPPVLSAVAQQRARVGVEPADAKAHVPRQPGGLRVATFVNLAAG"
     gene            4579..5526
                     /locus_tag="VFA35_05765"
     CDS             4579..5526
                     /locus_tag="VFA35_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HZT55715.1"
                     /translation="MPGTTRIEAIRRFNRFYTRRIGVLHEGLLDSAFTLTESRLLWEL
                     AHADGLTATQLARTLELDAGYLSRLLTGLKERGLIQGTRSADDARQQHLALTPAGRRA
                     FAPLDARSRADVSALLASLDAPQQQELLASMSTIERLLGAAPAARAEPYTLRPHRAGD
                     IGWVVSRHGALYAAEYGWDMRFEALVARIAADFIDRFNARREACWIAERGGERIGSIF
                     LMQARDEATQAPLDGIAQLRLLLIEPSARGLGLGKRLVDACTRFARQAGYRKIMLWTN
                     ANLLAARGIYAKAGYALTHTEQHRSFGHALVGEIWELEL"
     gene            5526..5909
                     /locus_tag="VFA35_05770"
     CDS             5526..5909
                     /locus_tag="VFA35_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HZT55716.1"
                     /translation="MCPCRSGTDALPYAACCGRYHGGPLHLLAPTAEALMRSRYSAFV
                     LDALDYLRASWHASTRPASLSPNPPGLKWLGLEVRRQALIDADHATVEFIARHKLGGR
                     AARLHELSRFVREEGRWFYVDGEAT"
     gene            complement(5923..>6449)
                     /locus_tag="VFA35_05775"
     CDS             complement(5923..>6449)
                     /locus_tag="VFA35_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55717.1"
                     /translation="YTVGARSLFVTATAWAFIVLAALTSMSALVQNAAVASLMPGMQV
                     ATEHMPLLTGLLLGYLPWVVGMGLVMSLATLASAIGLLMRLDWARRSFIALLVVAIFA
                     NLLGLWLQQEVVQSVVSSTLASVAIPQQAMGVFGGFVTAARVMAVLMTLAGCAFLGWI
                     IRRLMSAGVRQEFA"
BASE COUNT          949 a         2331 c         2327 g          842 t
ORIGIN      
        1 ccgcacactg ttccgcggcg ccgggctcga tctggcggac ctgcgcgaat accagcactt
       61 tgatgacgtg cgccacatcg actggaacgt caccgcgcgc ctgcagcaac cttatgtgcg
      121 ccagttcacc gaagaccgcg agctgaccgc ctggttcctc gtcgacctct cggcgagcgt
      181 cgatttcggc tcgaacgagc ggaccaagcg cggcgtcgcg cgcgagttcg tcggcgtgct
      241 cgcgcgtctg ctgacgcgtc gcggcaaccg catcggtgcg ctgctctacg gcaccgaggt
      301 cgacaccgtg ctgccggccg gcagtggccg ccggcacgtg ctcgagctgc tgcagcgcat
      361 gggccggcgg cccgagcaga gcgcggccgg tgcgaccgac ctgcgcgatc tgctggtggc
      421 cgcgcaaggc atcgtcaagc accgcggcag cgtgttcgtg atctccgact tcatcagcgc
      481 gccgggctgg gagcgctcgc tggcgcagct cgcgacgcgg cacgaggtga ccgcggtgcg
      541 cgtgctcgat ccgttcgaga tggcgttgcc cgacatcggc ctcgtgacga tgcgcgacgc
      601 cgaaagcggc gagcagctcg tcgtcgacac ccacgaccgc ggctttcgcg cgcgcttcga
      661 ggctgcggcc gagcggcgcg agaccgcgct gcgcggggcc ctcgcgcgcg ccggcgtcga
      721 cacgctcgag ctcgcgaccg acgacgatct gctggatgcg atcctgcgct tcgccgacct
      781 gcgcaagcag cgcagccggc tcgcgaactg gagcggccaa cccggccgcg cgagcgtcgg
      841 cgcgcacctg tcggcacggt ctggagttgc cgcatgaggg ccgagcgccg ggccgccatg
      901 ggccacgaag aggcccacct cgtggacctc ggccggccct cgtcccctcg ggggaccgac
      961 caatgtactc attgggcgag gggccgtcat gactgattgg ccgagcctgc cggtgacctt
     1021 cttctggccc gagatgctct ggggtctgct cgccgtgcca ctgctggtgt tgctctacat
     1081 ctggctgctg cggcggcgca agaggtcggc gctgcgttat gcgagcctga gcctggtcaa
     1141 ggaggcgatc ggccagcgcg tcgcgtggcg ccgccacgtg ccgcccgcgc tgctgctgac
     1201 cgcgatcacc ggcctgttga tcgcggccgc gcggccggcg gcggtgatca ccttgccgac
     1261 ggcggagaag acggtcgtgc tggcgatcga cgtctcgggc agcatgcgcg ccaccgacgt
     1321 gcagcccgac cgcatgaccg ccgcgaagga agccgcgaag gccttcgtcg ccgacctgcc
     1381 gcgcagcgtg cgcatcgcgg tcgtgcagtt cgccggcacc gcagcggtgg tgcaggcacc
     1441 cacgctcagc cgcgaagatg tcgtcgccgc gatcgatcgc ttccagctgc agcgcggcac
     1501 cgcgatcggc agcggcatcg tgctctcgct ggcgacgctg ttccccgacg ccggcatcga
     1561 tctgtcgcag atcaccggcc agcgcgagat gccgcccgcc cccggcgaca agcccaggcc
     1621 ggcgttcacg ccggtcgcgc cgggctccta cgcttcggcg gcgatcatcc tgctgaccga
     1681 cggccagcgc accaccggcc ccgacccggt cgaggcggcg aagatggcgg ccgaccgcgg
     1741 cgtgcgcgtc tacacggtgg gcgtgggcac gaaagagggc gagacgatcg gcttcgaggg
     1801 ctggtcgatg cgcgtgcgac tcgacgaaga cacgctgaag aacatcgcca acctgacgca
     1861 cgccgattac ttctacgccg gcaccgcgaa cgacctgaag caggtctatc agaacctctc
     1921 gacccggctc gtggtcgaga agaaggagac cgagatcagc ggcttgttcg cggcggtggg
     1981 cgcgttgctg gtggtggtgt cggcagggct gtcagtgtgg tggttcgggc gggtcgcgtg
     2041 atcacccgaa ccggccctgc aggaaccagt tgagcttcac gccgctcgtc ggcacgcgct
     2101 cgcggaagat ccagaccagc gcgccatccg gcaactgcgc gatgtagtaa tcgcgcgcaa
     2161 cgacgtcgaa atcccaccag cccgattcga tgcgctcggg gccgctgagc aggcgcagcg
     2221 gctggccgtc ggcccacggg ccgcgttcgc cgccgggcac ggcgcgctcg tgcagcggct
     2281 cgggccgctg caacagccag accggccgca tcggccgcgc cgcacccgcg gcttcaggcg
     2341 cgccggcagg cccgcctggg ctgcgcgcac gcgccatctg cgcgtcggcc gcgcggcaag
     2401 cggtcgcacg ctcggggcgg tggtctggca ccgacaccag ccgctgcacc tgcgcgcggc
     2461 cgaggcgggc ctgcaggcgc tcgatcaggc gggtcaggcc ctcgtgctcg gcggcgggtg
     2521 tggggaagag ttcggtgttc ggcggcgcgc ggcgcgcgat gtcgtcggcg tgcaggcgca
     2581 ggtcgagcgt cggcgcgggc agttgcagtt gcgcgaagcg ctcgcgcagc agcacctgca
     2641 ggtgggcgct gtcgcgcgag ggctcggcga gcgccacttc aaggcttgtg atgccgctac
     2701 cgagcgcgtc gtggcgcgag cgccgttcgt gcgccatcac cagcgtgaag cggcgcacga
     2761 acgcgtgctg cgccgcgagc caggccacca gccgcgccag cagcaccgcg gcgccgtgca
     2821 tcagctgctc ggtggtgtcg gcgcaggcga aaagttcgag ccggctgtcg aacgccggcg
     2881 gcagcgcaag ggacacgcgc gggtcggggc gtcggcccag cgcgcggtcg agcgcgtcga
     2941 gcacggccgg gccgaagcgg cgcgccaggc cgtcgcgcgg cacgcgctgc aggtcgccga
     3001 gggtgtgcag tcccatgccc tgcagcgcct cccgttgcgc gcggccgggg ccgagcagcc
     3061 acacgggcgc atgggccagc gcgcgggttg tcgcggcgag gtcggcgcag gccagcttcg
     3121 ggtgcacgcg ggcgagcagc gcggcgccga gcggcgtgct ggcgctgacg atgtgcaggt
     3181 ggtggccgag cggcgcgagc gcgctcgtca agcgctgctg cagcgccgcc aggccgccga
     3241 aatagcgcag gctggcctgg acttcgagca gcacggtgtg cggcgtgtcg cccggcgcgg
     3301 ttgtggccgc ggtggtggag ccagcatcgc cgggcggctc gatcacgacc gtcggcgtga
     3361 acgccagcgc cgcgtgtgcg accgccatca acgcctgcgc gtcgcgaacc gggtcggcca
     3421 ggccgaacgt gagctgcggc gccagtgcca gcgcggtcgc gcgcttgaag ccaggctgca
     3481 cgccgagcgc gcgcgccggc gcgttgacgg cgacgatgtg ctgcgcgtcc agcaacgcga
     3541 gcggccgctc gcccgcggcg tgcggcaagg tggccgcgaa cgattcgagc gagagcagcg
     3601 gcaggtgcag ggcgatccag agcgtcatgt cgctcgcggc gcgctcggcc ggttcacccg
     3661 gcggcgaggt tgacgaaggt ggcgacacgc agcccacccg gttgtctcgg cacatgcgcc
     3721 ttggcatccg ccggctcgac acccaccctc gcccgctgct gcgccaccgc cgagagcacc
     3781 ggcggcagcg cgagctgcag cggcgcctcc agcggcggcc cgcggcgctt gagcacgcgc
     3841 accgcgagca catcggggcc gtgggcgcgc aacgcgagcc gcagcggcgc ggcagtgggg
     3901 cgctgcgccg cgctcgtctc gcagaacacg aaagccgggc cgtcgtgcgc gtgcgcggcc
     3961 agctgcagcc ggcgcaggcg ctcggcccgc aggcgcggcg gcagccacgc gagcaccgcg
     4021 ccgacatggc cgctcttgag tgcctgctcg agcgcccaca ggctgtcggt gccgggaacg
     4081 acgcgggtgc gggtgtgtgt gtcgatgatc agcagctctt cgaccgcgat accgagctgc
     4141 gcgagcgcac tggccgacaa agtggccggc gggtcgaaca acatcaccag ccggcccgcc
     4201 acctgcgcgg cgaccaggct cggcgcgacc agccggatct cgccgacgcc cgggtgcggc
     4261 agcagcagtt cggtgagcgc gcggcgcggc cagccggcgc cgggcaattg cgcgtcgagc
     4321 gcttcgaacc cggtcgcgat gggcacatcg gcatggcgag cgagctggtg gccgagccac
     4381 agcgccgggt gcaggcgctc ggggcggatc ggcggcgggc gctgccgcgc gccacccatc
     4441 cccagaagcg gcaacggggg tgcggaaacg gacagcaagt tcatttgaac aactgtacgt
     4501 ttgtacagcc atcagcgcaa gcttcgggcc ttcaaactga ttgcttttag caagtatctg
     4561 cgaaagaatc ggccgaccat gcccggcacc acccgcatcg aagccatccg gcgtttcaac
     4621 cgcttctaca cccgccgcat cggcgtgctg cacgagggcc tgctggattc ggctttcacg
     4681 ctgaccgaat cgcgcctgct gtgggagctg gcgcatgccg acggcctcac cgcgacgcag
     4741 ctggcgcgca cgctcgaact cgacgccggc tacctgagcc ggctgctgac cggcctgaag
     4801 gaacgcgggc tgatccaggg cacacgctcg gccgacgacg cgcgccagca gcaccttgcc
     4861 ctcacccccg ccggccggcg cgcgttcgcg ccgctcgacg cgcgctcgcg cgccgacgtg
     4921 agcgcgctgc tcgccagcct cgacgcgccg cagcagcagg aactgctcgc gtcgatgagc
     4981 acgatcgagc gcctgctcgg cgccgcgccg gccgcgcgcg ccgagccgta cacgctgcgc
     5041 ccgcaccgcg ccggcgacat cggctgggtg gtctcgcgcc acggcgcgct ctacgccgcc
     5101 gaatacggct gggacatgcg cttcgaggcg ctggtcgcac gcatcgcggc cgacttcatc
     5161 gaccgcttca atgcccgccg cgaagcatgc tggatcgccg agcgcggcgg cgagcgcatc
     5221 ggcagcatct ttctcatgca ggcacgcgac gaggccacgc aggcaccgct cgacggcatc
     5281 gcgcagctgc gcctgctgct gatcgagccc tcggcgcgcg gcctgggcct gggcaagcgg
     5341 ctggtcgacg cgtgcacgcg tttcgcgcgc caagccggct accgcaagat catgctctgg
     5401 accaacgcca acctgctcgc cgcgcgcggc atctatgcga aggccgggta tgcgctgacg
     5461 cacaccgagc agcaccgcag cttcggccac gcgctggtcg gcgagatctg ggagctcgaa
     5521 ctgtgatgtg cccgtgccga agcggcaccg acgccctgcc ctacgccgcc tgctgtggcc
     5581 gctaccacgg cgggccgctg cacctgctgg cgccgaccgc cgaagcgctg atgcgatcgc
     5641 gctacagcgc cttcgtgctc gacgcgctcg actacctgcg ggcgagctgg cacgcgagca
     5701 cgcgccccgc atcgctgagc cccaacccgc cgggtctgaa atggctgggt ctggaagtgc
     5761 gccgccaggc cctgatcgac gccgaccacg cgacggtcga attcatcgcc cgccacaagc
     5821 tcggcggccg cgccgcgcgc ttgcacgaac tcagccgctt cgtgcgcgaa gaagggcgct
     5881 ggttctacgt cgacggcgag gccacctgac gcccccatca attcaggcga actcttgccg
     5941 gacgcctgcc gacatcaggc gccggatgat ccagccgaga aacgcgcagc cggccagcgt
     6001 catcagcacc gccatcacgc gcgccgcggt gacgaagccg ccgaacacgc ccatcgcctg
     6061 ctgcgggatc gcgacgctgg cgagcgtgct gctgaccacc gactgcacca cctcctgctg
     6121 cagccacagg ccgagcaggt tcgcaaagat cgccaccacc agcagcgcga tgaagctgcg
     6181 gcgcgcccag tccaggcgca tcagcaggcc gatcgccgag gcgagtgtgg ccagcgacat
     6241 caccaagccc atgcccacga cccagggcag gtagccgagc agcaggccgg tgagcagcgg
     6301 catgtgttcg gtggccacct gcatgcccgg catcaaggac gcgaccgccg cgttctgcac
     6361 cagcgccgac atgctcgtca gcgccgccag gacgatgaac gcccaggccg tggcggtgac
     6421 gaacagcgaa cgggctccaa cggtgtagg
//
LOCUS       DASRDG010000347         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_55085, whole genome shotgun sequence.
ACCESSION   DASRDG010000347 DASRDG010000000
VERSION     DASRDG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55085"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFA35_05780"
     CDS             <1..427
                     /locus_tag="VFA35_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dinucleotide-binding protein"
                     /protein_id="HZT55718.1"
                     /translation="CGTSIDGKTVIDTTNPIADRPPEQGVLTFFTGPGESLLEKLQQR
                     APKAKFVKAFSCVGAGLMVDPDLGDLKPTMFICGNDQKSKTQVAAILDEFGWETEDMG
                     PAAAARGIEPLCLLWCIPGFQRNDWTHAFKMLRPPRKSR"
     gene            555..>2328
                     /locus_tag="VFA35_05785"
     CDS             555..>2328
                     /locus_tag="VFA35_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZT55719.1"
                     /translation="MSVTSLRLRLILAFSALVSLILFVGLVAYGVNDQVRTDVAELRA
                     SGTIDLRYVKVGGNGLEIEGYWNPGGAFVATRVTQEPMPRRHPLLRGTIQSVSAAGRQ
                     LAMYGVPVLVTDDTRSADDKNQIRIESLRPGMRAELTCAVDNGSWRALKVQLDDVKPT
                     DKIKGTATKAEIDGVPPETIEIHGLKVVLEPQTERGTASALSRIAQATRMLEALQDCR
                     AAAHVFVESAPHGAPAAQVVEHADAELRSALDRFDGALQEAQTGQGLDSAPARELMGL
                     LDLLTSKRRHLRRYADEVRRLRDADPAEARSYLAATFGPFLDEMLRYVYSYLDRSGEQ
                     LRDEIRDVQGHTETTTRVALGTCGVAIAVALVLGILVWRSVHRPIQVLHDAALALGQG
                     RLDTRVAISSRDEMGVLADAFNKMAGQLEATTVSVTSLENMFASMAAGVILCDQQGRI
                     TNSNRAAQALLDRDRGDLVGLPFAAVCPGTAATAPGDQPGSVERAFLRRDGTELPVSL
                     SVSELRSAGGALQGKVYVAQDLTPLKRIEAQLRESLGEKELLLREVHHRVKNNMQVIS
                     SLLAMQSTSGDPQVRERLDESQHRI"
BASE COUNT          357 a          852 c          813 g          306 t
ORIGIN      
        1 ctgcggcacc tcgatcgacg gcaagaccgt catcgacacg accaacccga tcgccgaccg
       61 tccgcccgag cagggcgtgc tgacgttctt caccggcccg ggcgagtcgc tgctcgagaa
      121 actgcagcag cgcgcgccga aggcgaagtt cgtgaaggcg ttctcgtgcg tgggcgccgg
      181 cctcatggtc gaccccgatc tcggcgacct gaagccgacg atgttcatct gcggcaacga
      241 ccagaagagc aagacgcagg tcgccgcgat cctcgacgag ttcggctggg agacggagga
      301 catggggccc gccgcggccg cgcgcggcat cgagccgctg tgcctgctgt ggtgcatccc
      361 cggcttccag cgcaacgact ggacgcacgc gttcaagatg ctgcggccgc cgcgcaagtc
      421 gcgctgacgc gaggacgacg cggcggcccc gcgcgaacgc acgctgccac ggcgtcttcg
      481 cgcggacgtc gccgccgcgc cgatcgcgga ctactctttc tccgaggcct tcgcctctcc
      541 ctgccccctt gcgcgtgtcc gtcaccagcc tgcgcctgcg cctgatcctg gccttctcgg
      601 ccctggtctc gctcatcctg ttcgtgggcc tggtcgcata cggcgtgaac gatcaggtgc
      661 gcaccgacgt cgccgagctg cgcgcgagcg gcacgatcga cctccgctac gtcaaggtcg
      721 gcggcaacgg cctcgagatc gagggctact ggaacccggg cggcgcgttc gtggcgacca
      781 gggtgacgca ggagccgatg ccgcgccgcc atccgctgct gcgcggcacc atccagtcgg
      841 tctcggccgc cggacgccag ctcgcgatgt acggcgtccc ggtgctcgtc accgacgaca
      901 cgcggtcggc cgacgacaag aaccagatcc gcatcgagtc gctgcggccg ggcatgcgcg
      961 ccgagctcac ctgcgccgtc gacaacggca gctggcgcgc gctcaaggtg cagctcgacg
     1021 acgtcaagcc gacggacaag atcaagggca cggcgaccaa ggcggagatc gacggggtgc
     1081 cgcccgagac gatcgagatc cacggcctga aggtggtgct cgaaccgcag accgagcgcg
     1141 gcaccgccag cgcgctgtcg cggatcgcgc aggcgacgcg gatgctcgag gcgctgcagg
     1201 actgccgcgc ggccgcgcac gtgttcgtcg agtcggcgcc gcacggcgcc ccggccgcgc
     1261 aggtggtcga gcacgcggat gcggagctgc gctctgcgct ggacaggttc gacggcgcgc
     1321 tgcaggaggc gcagacgggg caggggctcg actcggcgcc ggcgcgcgag ctgatgggcc
     1381 tgctcgacct gctgaccagc aagcggcgcc acctgcgccg ctacgcggac gaagtgcggc
     1441 ggctgcgcga cgccgacccc gccgaggcgc ggtcctacct cgccgccacg ttcggcccgt
     1501 tcctcgacga gatgctgcgc tacgtctact cgtacctcga ccgcagcggc gaacagctgc
     1561 gcgacgagat ccgcgacgtg cagggccaca ccgagacgac gacgcgcgtt gcgctcggca
     1621 cctgcggcgt ggcgatcgcg gtggcgctcg tgctcggcat cctcgtgtgg cggtccgtgc
     1681 accggccgat ccaggtgctg cacgacgccg cactcgcgct gggccagggc cggctcgaca
     1741 cgcgcgtcgc gatcagctcg cgcgacgaga tgggcgtgct cgccgacgcg ttcaacaaga
     1801 tggccggcca gctcgaagcg accaccgtct cggtgacgag cctcgagaac atgttcgcgt
     1861 cgatggccgc gggcgtgatc ctgtgcgacc agcagggtcg catcaccaac agcaaccgcg
     1921 ccgcacaggc gctgctcgac cgcgaccgcg gcgacctcgt gggcctgccg ttcgccgccg
     1981 tctgccccgg cacggcggcc accgcgcccg gcgaccagcc gggcagcgtc gagcgcgcgt
     2041 tcctgcgccg cgatggcacc gagctgcccg tgtcgctctc cgtgtcggag ctgcgctccg
     2101 ccggcggcgc gctgcagggc aaggtctacg tcgcgcagga cctgacgccg ctgaagcgca
     2161 tcgaagcaca actgcgcgag tcgctcggcg agaaggagct gctgctgcgc gaggtccacc
     2221 accgcgtcaa gaacaacatg caggtgatca gcagcctgct cgcgatgcag tcgaccagcg
     2281 gcgacccgca ggtccgcgaa cgcctcgacg agagccagca ccgcatcc
//
LOCUS       DASRDG010000348         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_858057, whole genome shotgun sequence.
ACCESSION   DASRDG010000348 DASRDG010000000
VERSION     DASRDG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858057"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VFA35_05790"
     CDS             complement(<1..1362)
                     /locus_tag="VFA35_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZT55720.1"
                     /translation="MSLRSSQRVFGVRLGGWRARFPHLAGDLRGGLASAIVGLPYAVT
                     AAMLAFAPLGPGYVGHGMLAGLVTALVAGLVVIGGTPFQISGPRASVAVLMASIVALA
                     LAHPALGDAGAAQGPRVLGVLVLCLMMAGAIQVAFGVLGMGSALRFLPYPVISGFMVG
                     LGILVAAPQVPALLGAPGSGDWWSAVHQLPAMRPGALLVGLVTIATLVQVRKYLPKWP
                     APVAAMLAGTLLHYALASVSLFRVGPVPWDLVNGSFPLLPWEVPKVVLDSATLSVLID
                     LLPATLTLAFVGSLETLLSSSVLAITSNTRYDSRRELIGQGISNIAIAAAGGVASAAA
                     PFRGVTNYNAGGRTRLSVAVNGLLIGGVGLAAAPLLFWLPLAAWAGVLAVVGWDVAAA
                     WARRLVGNPRADVAVGLLVMLVTLALGTVPAILVGIAGCVFLHVHNTSRVPIRGSYDG
                     TSRA"
     gene            complement(1359..>2074)
                     /locus_tag="VFA35_05795"
     CDS             complement(1359..>2074)
                     /locus_tag="VFA35_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZT55721.1"
                     /translation="QLAPSGTLRVAVLTSNPIIGSKDASSGELKGTTVELGRALAASA
                     GMPVRMIDYAAIPKLMEDAGTNAWDVAVVAIDPARRSLVDFAPAHLAADGFLTILVPP
                     GSSARTMADFDRPGMRVAAVKNAAPLMILERTLKNAKVVAADNENEAFGLMRDGKAEG
                     YAQNRFILRTRAQTLPGSRLLDDAFSGLRLAFALPKNRPEAAKYVAAFVEDAKKSGEI
                     QRAIDKAGLAGEVKVAPPE"
BASE COUNT          359 a          743 c          718 g          254 t
ORIGIN      
        1 agcacgggaa gtgccgtcgt agcttccgcg gatcggcacg cgactggtgt tgtgcacatg
       61 cagaaacacg catccggcga ttcccaccag gatcgccggc accgtgccca gcgcgagcgt
      121 gacgagcatg accagcaggc cgaccgctac gtccgcgcgc ggattcccca cgaggcgccg
      181 cgcccatgcc gccgcgacat cccagccgac caccgcgagc acgccggccc aggcagcgag
      241 cggcagccag aagagtagcg gcgccgcggc gaggccgact ccaccgatca ggaggccgtt
      301 cacggcgacc gacaggcgcg tgcgcccgcc ggcgttgtag ttggtcacgc cgcgaaacgg
      361 cgcggcggcg ctcgccacgc cgcccgccgc tgctatggcg atgttcgaga ttccctggcc
      421 gatcagctcg cgccggctgt cgtagcgggt gttgctggtg attgcgagca ccgacgagga
      481 aagcagcgtc tccagcgatc ccacgaaagc gagggtcaac gtagcaggca gcaggtcgat
      541 cagcaccgac aaggttgcgc tgtcgagcac gaccttgggg acttcccagg gcagcagcgg
      601 aaaactgccg ttgaccaggt cccagggaac cggcccgacg cggaacaagg agacggacgc
      661 gagcgcgtag tgcagcagag tgccggcgag catcgccgcc accggcgccg gccacttcgg
      721 caggtacttg cgcacctgta ccagcgtggc aatggtaacc aagccgacga gcagcgcgcc
      781 gggccgcatg gccgggagct ggtgcaccgc gctccaccag tcgccggacc ccggcgcgcc
      841 gagcagcgcc ggaacctgcg gtgcggcaac cagaatgccc agcccgacca tgaagcccga
      901 gatcaccggg tagggaagga agcgcagcgc gctgcccatg ccaagcacgc caaaggcgac
      961 ttggatggcg ccggccatca tcaggcaaag cacgagcaca ccgagcacgc gcggaccttg
     1021 cgccgcgccg gcgtcgccca atgcggggtg tgcaagcgcg agcgcgacga tgctggccat
     1081 cagcaccgcg acggaggccc gggggccgct gatctggaag ggcgtgccgc cgatcacgac
     1141 caggccggcg acgagcgccg tgaccaggcc ggcgagcatg ccgtgcccta cgtaacccgg
     1201 gccgaggggc gcaaaggcca gcatcgccgc ggtgaccgcg taaggcagcc ctacgatcgc
     1261 actcgccagg ccgccgcgaa gatccccggc aagatgggga aaacgcgcgc gccatcctcc
     1321 caatcgcacg ccaaagacgc gctgcgaact cctgaggctc actcgggcgg agcgaccttg
     1381 acctcgccgg cgaggccggc cttgtcgatc gcgcgctgaa tttcgcccga cttcttcgca
     1441 tcctcgacga acgcggcgac atacttcgcc gcctcgggcc ggttcttcgg cagcgcgaac
     1501 gcgaggcgca ggcccgagaa tgcgtcgtcg agcaggcgcg agcccggcaa cgtctgcgcc
     1561 cgcgtgcgca ggatgaaacg gttctgcgcg tagccctcgg ccttcccgtc gcgcatcagc
     1621 ccgaacgcct cgttctcgtt gtccgccgcc acgaccttgg cgttcttgag cgtgcgctcg
     1681 aggatcatga gcggcgcggc gttcttcacc gccgcgacgc gcatcccggg ccggtcgaag
     1741 tcggccatgg tgcgcgcgct cgagccgggc ggcacgagga tggtgaggaa accgtcggcc
     1801 gcgaggtgcg ccggcgcgaa gtcgaccagc gagcggcgcg ccgggtcgat cgccaccacc
     1861 gcgacgtccc aggcgttcgt gcccgcgtct tccatcagct tcgggatcgc cgcgtagtcg
     1921 atcatgcgga ccggcatccc ggcgctcgcc gcgagcgcac ggccgagctc gaccgtggtg
     1981 cccttcagct cgccgctcga ggcgtccttc gaaccgatga tggggttcga cgtcagcacc
     2041 gcgacgcgca gcgtgcccga cggcgcgagc tgcg
//
LOCUS       DASRDG010000349         4466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_979000, whole genome shotgun sequence.
ACCESSION   DASRDG010000349 DASRDG010000000
VERSION     DASRDG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979000"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFA35_05800"
     CDS             <1..843
                     /locus_tag="VFA35_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55722.1"
                     /translation="FITLRRMWHTVAVIDDDRTAQARELDQVRSTDGGFWAALWEARA
                     SAFAGRVTAAHQLFQLGIQTATRDGMREIAGQSAAEDAETHAIAGQCADARREVAAAL
                     ASSRDNFTLERSSRVLALCGLAEESQRLTAELANRYANASLTTLIQRPVTAAALALQR
                     GQAQRAITLLDPVALYDRAPASEFWPAYLRGQAFLQLKDAQSAGAQFQHILDRRGQAP
                     LSPLYALAQLGRARAHALAGERDASRRAYERFFTLWESADAAHESLQAARREYARLPT
                     SRPG"
     gene            849..3785
                     /locus_tag="VFA35_05805"
     CDS             849..3785
                     /locus_tag="VFA35_05805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZT55723.1"
                     /translation="MSVHARAKDIFLDAVAISAQDRRAFVEQACGGDAALRDEVESLL
                     KFHDDESTNEAEPVPAGAGLAPGAVIAGRYRMIARIGQGGMGDVWRADDLVLDTPVAL
                     KMILAAGPDARDRLVNEVRLARQITHPAVCRVFDVGEADGLVFFTMELVRGEDLAALL
                     RRVGRLPPDRVIDIARQLCAGLAAAHAQSVLHRDLKPANVLIDGDGCVRITDFGIAIT
                     KSNTSGHALTGTPAYMAPEQRAPGMLVSERTDVYSLGLVLYELLVGHEAYKRWSTLDE
                     PLRPTAIVTNVDPALERVVMQALAREPRDRWASVEEMAAALPRPVPSPLSAPTASAGA
                     FGVRPRPFWLAGLGLAALVAILVYASAFVRTPDARTLTEQDVVLLTDFENSTGETVFD
                     GALKVALAVALEQSPFLKVFPEEQARQTLRLMQRPADERITRTVGRDIAQRERLKALL
                     AGSIASLGRSYVLTLEAINAETGDVMAREQAQAPTKEQVLAAMGTASASLRRKLGESL
                     ASVQKFDVPLPRATTTSLDALNAYSLALYNGREVPRLEAIPHLKRAIELDPTFAMAYA
                     LLSEVYANTGQSAFAPEVARRAFDLRDKVSDREQFFISWRYYRDALQAWDKALDVART
                     WTATYPREAFAFNSLGNALIRFGQFEPAVPALREAIRLDPQFIPAYSNLAASLLALNR
                     LPEARAILRQAADRHLEFGGARRMSFLMAFVEGDSATMERELTASLGVRATNAAFGWQ
                     AHALAFGGHINEAHDQYRRGIDRAAAGNFKEVAAQLSIEDAEMHATVGQCDKTKAEIP
                     AGLALSGDAAALDRASRALALCGGSDALALSAEVAKRLPEAILITRVSAPVTAAMLAL
                     ARGESKRALELLEAVRTYDRAPAAELWPVYVRGQAYLQSKNGAAARQEFQTILDHRGQ
                     LPVSLLYPLAHLGAARAATLLGDPEAARRSYDSMLEVWKAADPNLTPLDEVRRERAAL
                     RRTTTS"
     gene            3782..4330
                     /locus_tag="VFA35_05810"
     CDS             3782..4330
                     /locus_tag="VFA35_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZT55724.1"
                     /translation="MTDKPPDPHRDLFPEIYRELHRVAGGYLRRERRNHTLQPTALVN
                     EAWIRIQKEKGYELQGRTHGLAIAAQAMRRLLIDHGRHQKRDKRGGGVQPVELNDLMN
                     AAAAGPVPVEDLLTLEAALTRLEAIDPRAAEIVTLRFFSGMSSREVSEHLGLSLRTVE
                     GDWAHARAWLKRELSGGHKAEE"
BASE COUNT          679 a         1528 c         1579 g          680 t
ORIGIN      
        1 ttcatcaccc tgcggcggat gtggcacacg gtggcggtga tcgacgacga tcgcacggct
       61 caggcccgcg agctcgatca ggttcgaagc accgatggcg gcttctgggc tgcgctgtgg
      121 gaagcacggg cgtccgcgtt cgccggacgc gtgacggcgg cgcaccagct cttccagctc
      181 ggcatccaga cggcgacgcg cgatgggatg cgcgagatcg ccgggcagtc ggccgcggaa
      241 gacgccgaga cccacgcgat cgccggccag tgcgccgatg cccggcgcga ggtcgccgcc
      301 gcgctggcgt cgagccgcga caatttcacg ctggagcggt ccagccgggt gctcgcattg
      361 tgcgggctag ccgaggagtc gcagcggctg accgccgagc tcgccaaccg ttacgccaat
      421 gccagcctga cgacgctcat ccagcgcccg gtgacggctg cggcgctggc gctgcagcgc
      481 gggcaggcgc agcgcgcgat cacgctgctc gatccggtgg ctctgtacga tcgggcgccg
      541 gcctctgaat tctggccggc ctatctgcga ggtcaagcgt ttctgcagct gaaagacgcg
      601 cagtcggcgg gcgcgcagtt tcagcacatt ctcgatcgtc gcggtcaggc gcccctgtcg
      661 ccgctgtatg ccttggcgca gctcggacgg gcgcgcgccc acgcgctcgc cggcgagcga
      721 gacgcctcgc gacgcgccta cgaacggttc ttcaccctct gggagagcgc cgacgcggcg
      781 cacgagagtc tgcaggcggc ccgccgcgag tatgcaaggc tgcccaccag tcggcccggc
      841 tgattcccat gtccgtacac gctcgcgcca aggacatctt tctcgacgcg gtggcgatca
      901 gcgcccagga tcgccgtgcg ttcgtcgagc aggcgtgcgg cggcgacgcg gcgctgcgcg
      961 acgaagtcga gtcgctgctg aaatttcacg acgacgagag caccaacgaa gcggagccgg
     1021 tgccggcggg cgccgggctc gcgcccggcg cggtcatcgc cggccgttac cggatgattg
     1081 cccgcatcgg ccagggcggc atgggcgatg tgtggcgcgc cgacgatctc gtgctcgaca
     1141 cgccggtcgc gctgaagatg attctggcgg ccggtcccga tgcgcgcgat cgcctggtca
     1201 acgaagtccg gctggcgcgc cagattaccc acccggcggt gtgccgcgtg ttcgatgtcg
     1261 gcgaggccga cggtctggtc tttttcacga tggagctggt gcgcggcgag gatctcgccg
     1321 cgctgctgcg ccgcgtcggt cggctgcccc cggacagagt gatcgacatc gcccggcagt
     1381 tgtgcgccgg cctcgcggcc gcccacgctc agagcgtcct gcatcgcgac ctcaagcccg
     1441 ccaatgtcct gatcgacggc gacgggtgcg tccgcatcac cgatttcggc atcgcgatca
     1501 ccaagtcgaa taccagcggg cacgccctca ccgggacgcc ggcctacatg gcccccgagc
     1561 aacgcgcgcc cgggatgctg gtgtccgagc gcaccgacgt ctattcgctc gggctggtgc
     1621 tctacgagct gctggtcggc cacgaggcgt acaagcgttg gtcgacgctg gatgagccgt
     1681 tgcggcccac ggcgatcgtg accaatgtcg accccgcgct cgaacgcgtg gtcatgcagg
     1741 cactggcgcg cgagccccgc gaccgctggg cctcggtgga ggagatggca gcggcgctgc
     1801 cgcggccggt gccgtcgcca ctgagcgctc cgaccgcgtc ggcgggggcc ttcggtgttc
     1861 gtccgcggcc gttctggctc gccggcctgg ggctcgccgc gctggtcgcg atcctggtct
     1921 acgcctcggc gttcgtgcgc acgcccgacg cccgcaccct gaccgagcag gacgtcgtgc
     1981 tgctgaccga cttcgagaac tcgaccggcg agacggtgtt cgacggcgcg ctgaaagtcg
     2041 cgctggccgt cgcgctggaa cagtcgccgt tcctgaaagt ctttccggaa gagcaggcgc
     2101 gccagacgct gcggctgatg cagcgaccgg ccgacgagcg catcacgcgc acggttgggc
     2161 gcgacatcgc ccagcgcgag cggctgaagg cgctgctggc cggctcgatc gcgagtctgg
     2221 gccgcagcta cgtcctgacg ctcgaagcga tcaacgcgga gaccggcgac gtcatggctc
     2281 gcgaacaggc gcaggcgccg accaaggagc aggtgctggc tgcaatgggg accgcctcgg
     2341 cgagcctgcg ccgcaagctt ggcgagtcac tggcgtcagt gcagaagttc gacgtcccct
     2401 tgccccgtgc gaccacaacc tcgctggacg cgctgaatgc ctattcgctc gccctttaca
     2461 acgggcggga ggtgccccgg ctcgaagcaa tcccccattt gaagcgtgcg atcgagctcg
     2521 acccgacctt cgcgatggcc tacgcgctgc tgtcggaggt atacgcgaac accggacagt
     2581 ccgcgttcgc gcccgaggtc gcgcgcaggg ccttcgatct gcgcgacaag gtcagcgatc
     2641 gcgagcagtt cttcatctcg tggcgctact atcgcgacgc gctccaggcg tgggacaagg
     2701 cgctggatgt ggcgcgcacc tggacggcca cctacccgcg cgaagccttc gcgttcaaca
     2761 gcctcggcaa tgccctcatc cgcttcggcc agttcgagcc cgccgtgccg gcgctgcgcg
     2821 aggcgatcag gctggatcca cagttcatcc ccgcctactc caacctggcg gcctcgctgc
     2881 tggcgctcaa ccggctgccg gaggcgcggg cgattctccg gcaggcggcc gatcggcatc
     2941 tcgagttcgg cggcgcgcgg cgaatgtcct tcttgatggc gttcgtcgaa ggcgacagcg
     3001 cgacgatgga gcgcgagctg accgcgtcgc tcggcgtgcg ggctaccaac gcggcgttcg
     3061 gctggcaggc gcatgccctg gcgttcggcg ggcacatcaa cgaggcgcac gatcagtacc
     3121 gtcgcgggat cgatcgcgcc gcggcgggaa acttcaagga ggtcgcggcg cagttgtcga
     3181 tcgaagacgc cgagatgcac gccacggtcg ggcagtgcga caagacgaag gcggagatcc
     3241 ccgccggcct cgcgctgagc ggcgacgcgg ccgcgctcga ccgcgccagc cgggcgctcg
     3301 cgctgtgcgg cggctcggac gcgttggcgc tgtcggccga agtcgccaaa cggctgcccg
     3361 aggcgatcct gatcacgcgc gtctcggcgc cggtcacggc ggcgatgctg gcgctcgctc
     3421 gcggcgagtc gaagcgggcg ctcgagttgc tcgaggcggt gcggacctac gaccgcgcgc
     3481 ccgccgcgga actgtggccg gtctacgtgc gaggtcaggc gtacttgcag tcgaagaacg
     3541 gcgccgcggc gcgccaggag ttccagacca ttctcgatca ccgcggtcag ctcccggtgt
     3601 cgctgctcta tccgctggcg catctcggcg cggctcgagc ggcgacgctg ctgggcgatc
     3661 ccgaggcggc acgccggtcg tacgacagca tgctcgaggt ctggaaggcg gccgacccga
     3721 acctgacgcc tctcgacgag gtgcggcgcg aacgggcagc gctccgccgg actaccacgt
     3781 catgacagac aaaccaccgg atccgcaccg cgatctgttt ccggagatct accgcgagct
     3841 gcaccgggtg gctggtggtt acctgcgccg tgagcggcgg aaccatactc tgcagccgac
     3901 cgcgctcgtc aacgaggcct ggatccgcat ccagaaagag aaggggtacg aattgcaggg
     3961 gcgcacgcac ggcctggcga ttgccgcgca ggcgatgcga cggctgctga tcgatcacgg
     4021 ccggcaccag aagcgcgaca agcgtggtgg cggcgtccag ccggtggagt tgaacgatct
     4081 gatgaacgcc gccgcggccg ggccggtgcc tgtcgaggat ctgctgaccc tcgaggcggc
     4141 gctgacccgg ctcgaagcga tcgatccacg cgccgccgag atcgtcacgc tccggttctt
     4201 ctccggcatg tcgagtcggg aagtctccga acacctcggg ctctcgctgc gcacggtcga
     4261 aggcgactgg gcgcacgcgc gcgcctggct caagcgcgag ttgtccgggg gccacaaggc
     4321 ggaagagtaa cgacaccacc tcacacggga gtcgttctcc tggttgtcgg tgtcgatgtg
     4381 gtaggtcaag gtacaggaga atattgcact caaccgcgtg gcgcgaccga aactcctggt
     4441 cgcacgcgtc ggacccgggt gtataa
//
LOCUS       DASRDG010000350         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_625317, whole genome shotgun sequence.
ACCESSION   DASRDG010000350 DASRDG010000000
VERSION     DASRDG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625317"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFA35_05815"
     CDS             complement(<1..214)
                     /locus_tag="VFA35_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55725.1"
                     /translation="MVIKAVVTDIEGTTTSLAFVREVLFPYARQRLPAFVRARRAEPA
                     VAAQLAAAQQLAGRALDVDGLVQTLLQ"
     gene            complement(222..776)
                     /locus_tag="VFA35_05820"
     CDS             complement(222..776)
                     /locus_tag="VFA35_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HZT55726.1"
                     /translation="MTALTVFDEHGARLSQLSEPAPIAAQLADLGIEFARWPAAMLPH
                     DADLQAVLAAHAQPVARLGERHPIRSMDLVSLRPDHPDRAALRQRFLREHVHDDHEVR
                     FFAAGRGVFYMHMENRVFAALCGGGDFISIPAGVRHWFDMGSRPDFTCLRVFTAPDGW
                     VGRFTGSDIAARIPDFDALTAESP"
     gene            complement(773..1408)
                     /locus_tag="VFA35_05825"
     CDS             complement(773..1408)
                     /locus_tag="VFA35_05825"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:NF006672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HZT55727.1"
                     /translation="MSNRTAPADAEFEARTLELIAAGEAMFALGLMPATSGNLSCRLD
                     DGRIAITVSGCHKGLLSPADFVLVDAEGAARGERRPSAETALHAQIYRRCDGAGAVLH
                     AHALSAVVVSRGVQGTLELTGYELAKAFPGVTTHATTLVVPLFANDQDMGRLAQTVDA
                     WMATHAPVHGFLLAQHGLYTWGACVADALRHVEAFDHLFRCEFAWRTGRLL"
     gene            complement(1529..>3060)
                     /locus_tag="VFA35_05830"
     CDS             complement(1529..>3060)
                     /locus_tag="VFA35_05830"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT55728.1"
                     /translation="LAVTAAPLPAPAQGPAPPASLHGNDLHPNDVSTDLAYSDANFRN
                     KLDLWFPTDVKDAPLVVFVHGGAWIGGNKEMLGFVGHAFAQAGVACAVLNYRLWPLAR
                     GAHLADDVGTAFAWLHAHARERGCDPDRMFLAGHSAGGHLAALLAFDPARLAALGLPA
                     GAVKGFVGLSGVYDVRPEQDLLHKLFGRTPAERSAASPIVFAGPGAPPALLLWGEHDI
                     PGLDVSARILLSRLQLCGASAAGRAIAGNDHVDYVFRIGSERDVIGPELVSFVRTRCD
                     ELRRGAPPPVQRPFEVDVCRNARGREDAGARIDVYAPRNTARARPLLLVRGGDDGDAL
                     ARAAASWAARGLAVATLDLPLDVSGGERRRDEAVREIAAAAAWLRSHAAEFGADGARL
                     ALGGAGAGGSLALLAALDPAQLQAAGAPADAITAVIALGATVDPGAPARRDGPPALLL
                     WGDRDAEAAADAAFAARLRRAAHEVLTLELPGRDRKALLTGVGTGDDELSPLVLGYLA
                     R"
BASE COUNT          428 a         1107 c         1170 g          355 t
ORIGIN      
        1 attgcagcag cgtctgcacc agtccgtcga cgtcgagcgc gcgccccgcc agctgctgcg
       61 ccgcggcgag ctgcgcggcc acggccggtt cggcgcggcg cgcgcgcacg aacgcgggca
      121 gccgctgccg cgcgtagggg aacagcacct cgcgcacgaa cgccagcgac gtggtcgtgc
      181 cttcgatgtc ggtgacgacg gccttgatca ccatggcctg atcacggcga ctccgccgtc
      241 agcgcgtcga agtcggggat gcgggcggcg atgtcgctgc cggtgaagcg gccgacccag
      301 ccgtccggcg cggtgaacac gcgcaggcac gtgaagtccg gccggctgcc catgtcgaac
      361 cagtggcgca cgccggccgg gatgctgatg aaatcgccgc cgccgcagag tgctgcaaag
      421 acacggttct ccatgtgcat atagaagacc ccgcggccgg cggcgaagaa gcgcacttcg
      481 tggtcgtcgt gaacgtgctc gcgcaggaac ctctggcgca gcgcggcgcg gtcgggatgg
      541 tcgggccgca gcgacaccag gtccatcgac cggatcgggt ggcgttcgcc gaggcgcgcc
      601 accggctgcg cgtgcgccgc cagcaccgcc tgcaggtcgg cgtcgtgcgg cagcatcgcc
      661 gccggccagc gcgcgaactc gatgccgaga tcggcgagct gcgccgcgat cggcgccggc
      721 tccgacagct gcgagaggcg cgcgccgtgc tcgtcgaaca ccgtcagcgc ggtcacagga
      781 gcctcccggt gcgccaggcg aactcgcagc ggaacaggtg gtcgaacgcc tcgacgtgcc
      841 gcagtgcgtc ggccacgcag gcaccccacg tgtagaggcc gtgctgggcc agcaggaagc
      901 cgtgcacggg cgcgtgcgtc gccatccagg cgtcgaccgt ctgcgcgagg cggcccatgt
      961 cctggtcgtt ggcgaacaag ggcacgacga gagtggtcgc gtgcgtcgtc acgccgggga
     1021 acgccttggc cagctcgtag cccgtgagct ccagcgttcc ctgcacgccg cgcgaaacca
     1081 cgacggccga cagcgcgtgc gcgtgcagga cggcgccggc gccatcgcac cgccggtaga
     1141 tctgcgcgtg cagcgcggtc tcggccgagg gccgacgttc gccgcgcgcc gcgccttcgg
     1201 cgtcgaccag cacgaagtcc gcgggcgaca gcagaccctt gtggcagccg gagaccgtga
     1261 tcgcgatgcg gccgtcgtcg agccggcacg acaggttgcc gctggtcgcg ggcatcaggc
     1321 cgagggcgaa catggcctcg cctgcggcga tcagctccag cgtgcgcgct tcgaactcgg
     1381 cgtcggcggg ggcagtgcgg ttgctcacgc ggacatcgta gaacaggcgg cggtgccggg
     1441 ccggggcatc gtccgcaagt aacctgtccg ccatcgagcg gtaatctgcg cagcgcgatg
     1501 actgcgacgg gaaggctgcg cagcgtgctc agcgcgcgag gtagccgagc accagcgggc
     1561 tgagctcgtc gtcgcccgtg ccgacgcccg tcagcagcgc cttgcggtcg cggccgggca
     1621 gttcgagcgt caggacctcg tgcgcggcgc gccgcagtcg ggcggcgaac gcggcgtccg
     1681 ctgccgcttc ggcgtcgcgg tcgccccaga gcagcagggc gggcggcccg tcgcgccgcg
     1741 ccggcgcgcc agggtcgacc gtcgcgccga gcgcgatcac cgcggtgatg gcatcggcag
     1801 gcgcgccggc cgcctgcagc tgcgcggggt cgagcgccgc cagcagcgcc agcgaaccgc
     1861 cggcgccggc gccgcccagc gcgagccgcg cgccgtcggc gccgaactcg gccgcgtgac
     1921 tgcgcagcca ggccgccgcc gcggcgatct cgcgcaccgc ctcgtcgcgg cggcgctcgc
     1981 cgccggagac atcgagcggg aggtcgagcg tcgcgaccgc gaggccgcgc gcggcccacg
     2041 acgccgctgc gcgcgccagg gcgtcgccgt cgtcgccgcc ccggaccagc agcagcggcc
     2101 gcgcacgcgc cgtgttgcgc ggcgcgtaca cgtcgatgcg cgcaccggca tcctcacggc
     2161 cgcgcgcatt gcggcacacg tcgacctcga agggtcgctg caccggcggc ggcgcgccgc
     2221 gccggagctc gtcgcagcgc gtgcgcacga aggagaccag ctcggggccg atgacgtcgc
     2281 gttcgctgcc gatgcggaag acgtagtcga cgtgatcgtt tcccgcgatc gcgcgcccgg
     2341 ccgccgacgc gccgcacagc tgcagcctgc tgagcaggat gcgcgcgctg acgtcgaggc
     2401 cgggtatgtc gtgctcgccc cacaagagca gcgccggcgg cgcgcccggg cccgcgaaca
     2461 cgatcggcga cgcggcgctg cgctcggcgg gcgtgcggcc gaacagcttg tgcagcaggt
     2521 cctgctcggg ccgcacgtcg tagacgcccg acagcccgac gaaacccttc accgcgccgg
     2581 ccggcagccc gagggcagcc agccgcgccg ggtcgaacgc gagcagcgcg gcgaggtggc
     2641 cgccggccga gtggccggcg aggaacatgc ggtcgggatc gcagccgcgt tcgcgcgcgt
     2701 gggcgtggag ccacgcgaac gcggtgccga cgtcgtcggc gaggtgcgcg ccgcgcgcca
     2761 gcggccagag acggtagttc agcaccgcgc aggcgacgcc ggcctgcgcg aacgcgtggc
     2821 cgacgaaccc gagcatctcc ttgttgccgc cgatccacgc gccgccgtgc acgaacacga
     2881 ccagcggcgc gtccttcacg tcggtcggga accagagatc gagcttgttg cggaagttgg
     2941 cgtccgagta cgcgaggtcg gtggagacgt cgttcggatg caggtcgttg ccatgcaggg
     3001 acgctggcgg cgcgggaccc tgggccggcg ccggcagcgg agcggcggtg acggcgagca
//
LOCUS       DASRDG010000351         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_167379, whole genome shotgun sequence.
ACCESSION   DASRDG010000351 DASRDG010000000
VERSION     DASRDG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167379"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2117
                     /gene="uvrB"
                     /locus_tag="VFA35_05835"
     CDS             <1..2117
                     /gene="uvrB"
                     /locus_tag="VFA35_05835"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZT55729.1"
                     /translation="TRWRAPGRAASSPAMSARFELVTDMSPAGDQPAAIERLHGWLAG
                     GAHACTLLGVTGSGKTFTVARLVESLQRPTLVLAPNKTLAAQLYGEFKAFFPRNAVEY
                     FVSYYDYYQPEAYVPTTDTYIEKDALINEAIERMRNSATRSLMERRDVLIVASISCIY
                     GLGSPENYRELSVTVQKGGRVARDLLLRQLTAIQFVRDNFDFQPGRFRVRGDVIEVYP
                     SYEEAKAIRIELFGDEVEDVVEIDPLRGVVLKKLDRVTIYPTTHYVTTTEGLERACAS
                     IEQELAERLPWLEGRGKLLEAQRLGQRTRHDLEMMRATGLCQGIENYSRHLDGRAPGQ
                     PPATLLHYFPDDFLLVVDESHVAVPQSGGMYRGDQSRKGTLVEHGFRLPSALDNRPLK
                     FEEFEALQRQVLFVSATPDEYELKRSQDRIAELVVRPTGLLDPPVEIRPARGQVDDVV
                     GEVRACVQKQQRVLITTLTKRAAEELTDYLRDLGVRVRYLHSDIDSLERTALIRDLRL
                     HVFDVLVGINLLREGLDIPEVALVAVLDADKEGYLRSRTSLIQTCGRAARNVDGRVLL
                     YADKRTESIAAAVAEMERRRARQREFNERHQITPRTVVKPVRDLLEAVVGDGDEAAPK
                     GRQERSGRGRRGRGGAPAAGDGPVAAPGARPQFQSKLELLQHVKVLRAEMMAAAKNLD
                     FELAARIRDEVFRLEKLEMELL"
BASE COUNT          353 a          788 c          837 g          307 t
ORIGIN      
        1 acacccgttg gcgcgcgccg gggcgggctg catcatcacc ggcgatgagc gcccggttcg
       61 agttggtgac cgacatgtcg cccgcgggcg accagcccgc ggcgatcgaa cggctgcacg
      121 gctggctcgc cggcggtgcc catgcctgca ccctgctcgg cgtcaccggc tccggcaaga
      181 cgttcacggt cgcgcggctg gtcgagtcgc tgcagcgccc gacgctcgtg ctcgcgccga
      241 acaagacgct cgcggcgcag ctgtacggcg agttcaaggc cttcttcccc cgcaacgccg
      301 tcgagtactt cgtcagctac tacgactact accagcccga ggcgtacgtg ccgacgaccg
      361 acacgtacat cgagaaggac gcgctgatca acgaggcgat cgagcgcatg cgcaactcgg
      421 cgacccggtc gttgatggag cggcgcgacg tgctgatcgt cgcgtcgatc agctgcatct
      481 acggcctcgg ctcgccggag aactaccgcg agctgtcggt gacggtgcag aagggcggcc
      541 gcgtcgcgcg cgacctgctg ctgcgccagc tgacggcgat ccagttcgtg cgcgacaact
      601 tcgacttcca gcccggccgc ttccgcgtgc gcggcgacgt gatcgaggtc tacccgtcgt
      661 acgaggaggc caaggcgatc cggatcgagc tgttcggcga cgaggtcgag gacgtggtcg
      721 agatcgatcc gctgcgcggc gtcgtgctga agaagctcga ccgcgtgacg atctacccga
      781 ccacgcacta cgtgacgacc accgaggggc tcgaacgcgc ctgcgcgtcg atcgagcagg
      841 agctggcgga acggctgccg tggctcgagg ggcgcggcaa gctgctcgag gcgcagcggc
      901 tcggccagcg cacgcgccac gacctcgaga tgatgcgcgc gacgggcctg tgccagggga
      961 tcgagaacta cagccgccac ctcgacggcc gcgcgccggg gcagccgccg gcgacgctgc
     1021 tgcactactt ccccgacgac ttcctgctgg tcgtcgacga gtcgcacgtc gcggtgccgc
     1081 agtcgggcgg catgtatcgc ggcgaccagt cgcgcaaggg cacgctcgtc gagcacggct
     1141 tccggctgcc gagcgcgctc gacaaccggc cgctgaagtt cgaggagttc gaggcgctgc
     1201 agcggcaggt gctgttcgtg tccgcgactc ccgacgagta cgaactcaag cgcagccagg
     1261 accggatcgc cgagctggtc gtgcggccga ccggactgct cgatccgccg gtcgagatcc
     1321 ggcccgcgcg cggccaggtc gacgacgtcg tcggcgaggt cagggcgtgc gtgcagaagc
     1381 agcagcgcgt gctgatcacg acgctgacca agcgcgcggc ggaggagctg acggactacc
     1441 tgcgcgacct cggcgtgcgc gtgcgctacc tgcacagcga catcgacagc ctcgagcgta
     1501 cggcactgat ccgcgacctg cgcctgcacg tgttcgacgt gctggtcggc atcaacctgc
     1561 tgcgcgaggg cctcgacatc cccgaggtgg cgctggtcgc cgtgctcgac gccgacaagg
     1621 agggctacct gcgcagccgc acgtcgctga tccagacctg cgggcgcgcg gcgcgcaacg
     1681 tcgatggccg cgtgctgctc tacgccgaca agcgcaccga gtcgatcgcc gccgcggtcg
     1741 cggagatgga gcggcgccgc gcgcggcagc gcgagttcaa cgagcggcac cagatcacgc
     1801 cgcgcacggt cgtgaagccg gtgcgcgacc tgctcgaggc ggtggtcggg gacggcgacg
     1861 aggcggcgcc gaagggccgg caggaacggt cgggccgcgg ccggcgcggc cgcggcggcg
     1921 cgccggcggc gggggacggc ccggtcgcgg cgccgggcgc gcggccgcag ttccagagca
     1981 agctcgagct gctgcagcac gtgaaggtgc tgcgcgccga gatgatggcc gcggcgaaga
     2041 acctcgactt cgagctcgcg gcgcgcatcc gcgacgaggt gttccggctc gagaagctcg
     2101 agatggagct gctctagcgt cgccgatgca tccgcagatc gtcgacaagg tggcgcgctt
     2161 ccccgagggg ccgggcgtct acgtgttcgt cgacgaggac ggcaaggcgc tctacgtcgg
     2221 caaggccgcc gacctgcgcg cccgcgtcag gagctacctg aagccgggcg gcgacggccg
     2281 cttcc
//
LOCUS       DASRDG010000352         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_167432, whole genome shotgun sequence.
ACCESSION   DASRDG010000352 DASRDG010000000
VERSION     DASRDG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167432"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VFA35_05840"
     CDS             complement(<1..1357)
                     /locus_tag="VFA35_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZT55730.1"
                     /translation="MFDFFRKHTWLLQIVLGLVVLAFVAGGVYQGYGSFMGDDSTVAK
                     VDGRKITRNEWDIAQREQIERVRRQMPNVDPKVFDTPEMKRQSLDAVVRERVMLAAAD
                     TLHLTTTDERLQRLFVTDPQFAFLRNPDGSVNKDALSAQGMSSELFAQRLRQDLSLRQ
                     VVQGINGTAFAPLAATNAALDAMFQQREVQVQRFAAKDALAKVTPTDADIEAYYKNPA
                     HAAQFVAPEVENIEYVVLDLESLKKGIAVSDDDLRKYYAENAKRYTSTEERRASHILV
                     KVDPGASAADRAKAKTKAEGLLAEVKRNPASFAEVARKNSDDPGSAEKGGDLDFFGRG
                     GLAAKPLEDAAFKLKPDEISDLVESDFGYHIVKLTAVRGGEKKPFEAVRAELENEVKT
                     QLAQKKFSETAVEFTNMVYEQPDSLKPVVDKLKLELRTAQNVKRAPAPGATGALANPK
                     FL"
     gene            complement(1468..1543)
                     /locus_tag="VFA35_05845"
     tRNA            complement(1468..1543)
                     /locus_tag="VFA35_05845"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1508..1510),aa:Val,seq:tac)
     gene            complement(1590..1862)
                     /locus_tag="VFA35_05850"
     CDS             complement(1590..1862)
                     /locus_tag="VFA35_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZT55731.1"
                     /translation="MNKSELIEHIAKQADISKAAATRALEAVIGGVKSTLKKNNTVSL
                     VGFGTFSVGKRAARSGRNPRTGATIKIKAAKVPKFRPGKALKDALN"
     gene            complement(2126..>2297)
                     /locus_tag="VFA35_05855"
     CDS             complement(2126..>2297)
                     /locus_tag="VFA35_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZT55732.1"
                     /translation="VQMIAIPFLLFHGKLFGIIDTQRWGTWLIYASAVLTIWSMVYYL
                     RRALPEIRAKAR"
BASE COUNT          344 a          746 c          761 g          446 t
ORIGIN      
        1 cgaggaactt cgggttcgcg agcgcgccgg ttgcgccggg tgccggtgcg cgcttgacgt
       61 tctgcgccgt gcgcagttcg agcttgagct tgtcgaccac cggcttcagg ctgtcgggct
      121 gctcgtagac catgttggtg aactcgactg cggtctcgga gaacttcttc tgcgcgagtt
      181 gcgtcttgac ctcgttctcg agctcggccc ggaccgcctc gaacggcttc ttctcgccac
      241 cgcgcaccgc ggtcagcttg acgatgtggt agccgaagtc gctctcgacc aggtcgctga
      301 tctcgtcggg cttgagcttg aatgcggcgt cttcgagcgg cttcgcggca aggccgccgc
      361 ggccgaagaa gtcgaggtcg ccgcccttct cggccgagcc cgggtcgtcc gaattcttgc
      421 gcgcgacctc ggcgaacgac gccgggttcc tcttcacctc ggcgagcagg ccctcggcct
      481 tggtcttcgc cttcgcgcgg tcggcggccg acgcacccgg atcgaccttc acgaggatgt
      541 ggctcgcgcg ccgctcttcg gtcgaggtgt agcgcttcgc gttctcggcg taatacttgc
      601 gcaggtcgtc gtcggagacg gcgatgccct tcttcagcga ttcgaggtcg agcacgacgt
      661 attcgatgtt ctcgacctcg ggcgccacga actgcgccgc atgggccggg ttcttgtagt
      721 aggcctcgat gtcggcgtcg gtcggcgtca ccttcgccag cgcatccttg gcggcgaagc
      781 gctgcacctg cacctcgcgc tgctggaaca tcgcgtcgag cgcggcattg gttgcagcca
      841 gcggcgcgaa cgccgtgccg ttgatgccct gcacgacctg gcgcagcgag agatcctggc
      901 gcaggcgttg cgcgaacagc tccgacgaca tgccttgcgc cgagagcgcg tccttgttga
      961 cgctgccatc ggggttgcgc aggaatgcga actgcgggtc ggtgacgaac aggcgctgca
     1021 ggcgctcgtc ggtcgtcgtg aggtgcagcg tgtcggccgc ggcgagcatc acgcgctcgc
     1081 gcaccacggc gtcgagcgac tggcgcttca tctcgggcgt gtcgaacacc ttcgggtcga
     1141 cattgggcat ctggcgccga acgcgctcga tctgctcgcg ctgcgcgatg tcccattcgt
     1201 tgcgcgtgat cttgcggccg tcgaccttcg cgacggtgga gtcgtcgccc atgaagctgc
     1261 cgtagccctg gtacacgccg ccggcgacga aggccagcac gaccagcccg aggacgattt
     1321 gcagcagcca ggtgtgcttg cggaagaagt caaacatcga aagcctctat cggcgaaacg
     1381 tggaacccag atgcgacaaa ggcgaaccga ggttcgcctt tgtctgggtg gcggctgcga
     1441 ttctagccga gccgtcgggg tgtgatctgg tgggtgctga ggggttcgaa cccccgacct
     1501 acgccttgta agggcgccgc tctaccaact gagctaagca cccgcatccg catccgcatt
     1561 gcggcgctgc gtcggctcgc gaccgcggct cagttgagcg cgtctttcag ggccttgccg
     1621 gggcggaact tcggcacctt ggcggccttg atcttgatcg tcgcgccggt gcgcgggtta
     1681 cgcccgctgc gcgccgcgcg cttgccgacg ctgaacgtgc cgaagccgac gagcgacacg
     1741 gtgttgttct tcttcagcgt ggacttcaca ccgccgatca cggcctccag ggcacgcgtt
     1801 gccgccgcct tggaaatgtc ggcctgcttg gcgatgtgct cgatcaactc tgacttgttc
     1861 acaggtgtcc ccctcttaaa ggtatcacga tgaattcggg tgggtgttcg ggctgcaagg
     1921 ctggtgaggc ggcagctcga aacgattaca gccgcgcact cactcactgt caagcgcgga
     1981 agcggattct attcgtattc ccccgcctgg actcgatgcg aagccggcgc cttgtcgctt
     2041 gatcgcagcg ggtgcgttcg tatcgcacgc gcgctgccta aacatgggct cgcaaccgct
     2101 cgagcgccgc gcgagcaacg gccagtcagc gcgccttggc gcgaatctcg ggcagcgccc
     2161 tgcgcaggta gtagaccatc gaccagatcg tcagcaccgc cgacgcgtag atcagccagg
     2221 tgccccagcg ctgcgtgtcg atgatcccga acagcttgcc gtggaacagc aggaacggga
     2281 tcgcgatcat ctgcacc
//
LOCUS       DASRDG010000353         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_509257, whole genome shotgun sequence.
ACCESSION   DASRDG010000353 DASRDG010000000
VERSION     DASRDG010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509257"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VFA35_05860"
     CDS             <1..1572
                     /locus_tag="VFA35_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55733.1"
                     /translation="ELPLVSAVPAAQQLFARGMVQSYAFNDVEAARAFKAALAQDPNC
                     AMCAWGVAKADGPNINNTDRGDLTDARRYLAWATAHVAPSASRERALIEALRERYGPE
                     AGTAKAAVPEMPICSSGGAAKAHPLDIVYATRMRALADAYPDDPDILVLYAEAVMIAT
                     RPDWWDKKTGQPAGEIGIVTERLERAVAARPGHTGLNHFLIHAADSSPHPERAAAAAD
                     RLGGLAPASPHLLHMPAHIYVRLGRYRDAVRVNEEAIAAQARQKAALDAQGFSQSFDW
                     DGHNRDFLWFAALTEGRGELALAQARALAVRAAKGKSPTAEFIRGLPLLTLVRLERWG
                     EVLNEPVPAGEAGLATPMADYARGVALVRTGKLDAARERSAALQTALAKPVLKGATVM
                     GDDPAAKVLEILSSRLAAEIAAAEGRGDATQAALEHGIELEAALEATEPPLLASSSRL
                     ALGSLMLRLQRWSDAERAYRQELVAQPASGWALAGLEQALTGQGRAEEAQRTRTEAER
                     AWSAADASVRRIAMR"
     gene            complement(1588..>2312)
                     /locus_tag="VFA35_05865"
     CDS             complement(1588..>2312)
                     /locus_tag="VFA35_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZT55734.1"
                     /translation="APRSIALALSAWRGLYRWLGGEGLVAANPVEGVRAPRAAQPLPK
                     ALSVDDAVALVAHRDAAGSPVLAARDACIAELLYGCGLRVGELVGLDVVAGAGAAGWI
                     DAADASAHVLGKGSKRRSVPVGKPALQALAAWLAQRGEVARPGEPALFVSRRGTRLTA
                     SQVRSRLKAQALAAGLPTHVHPHMLRHSFASHVLQSSGDLRAVQELLGHANITTTQVY
                     TKLDFGHLSKVYDAAHPRARRK"
BASE COUNT          328 a          862 c          839 g          283 t
ORIGIN      
        1 gagctgccgc tcgtcagcgc cgttcccgcg gcacagcagt tgttcgcccg cggcatggtg
       61 cagtcgtacg cgttcaacga cgtcgaggcg gcgcgcgcct tcaaggctgc cctggcgcag
      121 gacccgaact gcgcgatgtg cgcctggggc gtggccaagg cggacggacc caacatcaac
      181 aacacggatc gtggcgacct caccgatgct cgtcgttatc tggcctgggc gaccgcgcac
      241 gtcgccccct ccgcgagccg cgaacgcgcc ctgatcgaag ccctccgcga gcgctatggt
      301 cccgaagccg gcacggccaa agcggcggtg ccggagatgc cgatctgctc gtcgggcggc
      361 gcagcaaagg cgcatccgct cgacatcgtc tacgcgacgc gaatgcgcgc tttggccgac
      421 gcgtaccccg acgatcccga catcctggtg ctgtacgccg aggcggtgat gatcgcgacg
      481 cgccccgact ggtgggacaa gaagaccggg cagcccgccg gcgagatcgg catcgtcacc
      541 gagcggctcg agcgcgcagt cgcggcgcgc cccgggcaca ccggactcaa ccacttcctg
      601 atccatgctg ccgattccag cccgcatccg gagcgcgccg ccgccgccgc cgaccggctc
      661 ggcgggctcg cgccggcctc gccgcacctg ctgcacatgc cggcgcacat ctacgtgcgc
      721 ctcggccgct atcgcgacgc ggtgcgcgtc aacgaggagg cgatcgccgc ccaggcgcgg
      781 cagaaggcgg cgctcgacgc ccaggggttc tcgcagagct tcgactggga cggtcacaac
      841 cgcgacttcc tctggttcgc ggcgctgacc gagggccgcg gcgagctggc cctggcccag
      901 gcgcgcgcgc tggccgtgcg cgccgccaag ggcaagagcc cgaccgccga attcattcgc
      961 ggcctgccgc tgctgacgct ggttcgcctc gaacggtggg gcgaagtgct caatgagccg
     1021 gtgccggccg gcgaagccgg gctcgccacg ccgatggccg actacgcgcg tggcgtggcc
     1081 ctggtgcgca ccggcaagct cgatgccgca cgcgagcgct ccgctgccct gcagaccgcg
     1141 ttggcgaagc cggtgctcaa gggcgcgacg gtgatgggcg acgaccccgc tgcgaaggtg
     1201 ctggagatcc tctccagccg tctcgccgcc gagatcgcgg cggccgaagg tcggggcgat
     1261 gccacgcagg cggcgctcga gcacggcatc gagctcgaag cggcgctcga agcgaccgag
     1321 ccgccgctgc tggcctcgtc gtcgcggctc gcgctcggca gcctgatgct gcgcctgcaa
     1381 cgctggtccg acgccgaacg cgcctaccgc caggagctcg tcgctcagcc ggccagcggc
     1441 tgggctctcg ccggactcga gcaggcgctc acggggcagg gccgggccga ggaagcgcag
     1501 cgcacccgta ccgaggccga gcgggcctgg agcgccgccg acgcgtcggt tcgccgcatc
     1561 gcgatgcgct gaaccgcccg tcgctgccta cttgcgccgc gcccgcggat gggcagcgtc
     1621 gtacaccttg gagagatggc cgaagtcgag cttggtgtag acctgcgtgg tcgtgatgtt
     1681 ggcgtggccg agcaactcct gcaccgcgcg caggtcgccg ctcgactgca ggacatgcga
     1741 ggcgaacgag tggcgcagca tgtgcggatg gacgtgcgtc ggcaagccgg cggcgagcgc
     1801 ctgcgccttc aggcgcgagc gcacctggct ggccgtcagc cgggtgccgc ggcgactgac
     1861 gaagagcgcc ggctcgcccg ggcgcgcgac ctcgccgcgc tgcgccagcc acgccgcgag
     1921 cgcctgcaac gcgggcttgc ccaccggcac gctgcgccgc ttgctgccct tgccgaggac
     1981 gtgggcgctg gcatcggccg cgtcgatcca gccggccgct ccggccccgg cgacgacgtc
     2041 gagaccgacc agctcgccga cccgcaggcc gcagccgtag agcagctcgg caatgcaggc
     2101 atcgcgcgcc gccagcaccg gcgagccggc agcgtcgcga tgcgccacca gcgccaccgc
     2161 gtcgtcgacc gagagcgcct tgggcaaggg ctgcgcggcg cgcggcgcgc gcacgccctc
     2221 gaccggattg gcggcgacca ggccttcgcc gccgagccag cggtagaggc cgcgccaggc
     2281 cgagagcgcc agggcgatgc tgcgcggcgc ca
//
LOCUS       DASRDG010000354         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1436844, whole genome shotgun sequence.
ACCESSION   DASRDG010000354 DASRDG010000000
VERSION     DASRDG010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436844"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..2043
                     /locus_tag="VFA35_05870"
     CDS             43..2043
                     /locus_tag="VFA35_05870"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZT55735.1"
                     /translation="MFLGSIIVSAAVFRLAAQAEPPPVPPPSLHGVRVPEVDDLDDYL
                     ASRQAAIVLGKALFWDQQAGSDGMACASCHFHAGADDRFVNQLSPSLNHQDPARAGVF
                     DTTHTGGGGPNYALRRGDFPFHVLSNPDDRDSSVLFDMNDVASSAGVFKADFVSVGSP
                     AGMTDSCQPRVDDLFHIGTLNTRRVEPRNTPTTINSAFNFRNFWDGRANNVFNGVDPF
                     GPRDTAARIKRWSDGRLVDVRVRLRNSSLASQAVGPPLSGFEMSCVGRTFPDLGRKLI
                     DRRALQFQSVAADDSVLAGHLHATRVGLVETYRTLIQRAFRSELWTAPGNVLTPAGFT
                     QIENNFSLFWGLAIQLYEGTLVSDDAPYDRFAAGDPNALTAAQRRGFDVFMGPGNCIA
                     CHKGPLFTGATLALFAPARAEEDQLPSPIERMLMGDGRLAIYDDAFYNIGVRPTVEDL
                     GVGGRDPFGNPLSFTRQWQQFLLTGSPTVDRFRVDPGQFAVPVPLPPSPTRAQLLAAF
                     GNDPEAVDGAFKVPTLRNVELTGPYFHNGGQATLEEVVRFYNRGGDRRRTGNGGANTT
                     GFGTNPSNLDPDIEPLGLSAQQQADLVAFLKSLTDERVRWEVAPFDHPQLAVMQGHSA
                     NVNPRLGAPMAADLGTVLPEVGRGGRAVLGLPALRPFADQLH"
BASE COUNT          300 a          831 c          755 g          306 t
ORIGIN      
        1 ggagcgttat tcgtgcctcc atatcctgac catcgcaaga ccatgttcct cggcagcatc
       61 atcgtgtctg ctgccgtatt ccggctcgcg gcccaggccg agccgccgcc cgtcccgccg
      121 ccgtcgctgc acggtgtccg cgtgcccgag gtcgacgacc tcgacgacta cctggccagc
      181 cggcaggcgg cgatcgtgct cggcaaggcg ctgttctggg accagcaggc cggcagcgac
      241 ggcatggcct gcgcgagctg tcacttccac gccggtgccg acgaccggtt cgtcaatcag
      301 ctgtcgccgt cgctgaacca ccaggacccg gcccgcgccg gcgtcttcga cacgacgcac
      361 accggcggcg gcggtcccaa ctacgcgctg cgccgcggcg acttcccgtt ccacgtgctg
      421 tcgaaccccg acgaccgcga ctcgagcgtc cttttcgaca tgaacgacgt ggcctcgtcg
      481 gccggcgtgt tcaaggccga cttcgtgtcg gtcggcagtc cggccgggat gacggacagc
      541 tgccagccgc gcgtcgacga cctgttccac atcggcaccc tgaacacgcg ccgcgtcgag
      601 ccgcgcaaca cgccgaccac gatcaactcg gcgttcaact tccgcaactt ctgggacggc
      661 cgcgccaaca acgtgttcaa cggcgtcgac ccgttcgggc cgcgcgacac cgccgcgcgc
      721 atcaagcgct ggagcgacgg ccggctggtc gacgtccgcg tgcgcctgcg caactcgagc
      781 ctcgcctcgc aggccgtcgg tccgccgctc agcggcttcg agatgtcgtg cgtcgggcgc
      841 acgttccctg atctcggccg caagctgatc gaccggcgcg cgctgcagtt ccagtccgtc
      901 gccgccgacg acagcgtgct ggccggccac ctgcacgcga cgcgcgtcgg cctggtcgag
      961 acctaccgca cgctgatcca gcgcgcgttc cgcagcgagt tgtggacggc gccgggcaac
     1021 gtgctgacgc cggcgggctt cacgcagatc gagaacaact tctcgctgtt ctggggcctg
     1081 gcgatccagc tctacgaagg cacgctggtc tccgacgacg cgccgtacga ccgcttcgcg
     1141 gccggcgacc cgaacgcgct gacggcggcg cagcggcgcg gcttcgacgt gttcatgggc
     1201 ccaggcaact gcatcgcctg ccacaagggc ccgctgttca cgggcgccac gctggcgctg
     1261 ttcgcgccgg cccgcgcgga ggaagaccag ctgccgagcc cgatcgagcg catgctgatg
     1321 ggcgacggcc ggctcgccat ctacgacgac gccttctaca acatcggcgt gcggccgacg
     1381 gtcgaggacc tcggcgtcgg cggccgcgat ccgttcggca acccgctgtc gttcacgcgc
     1441 cagtggcagc agttcctgct gacgggcagc ccgacggtcg accgcttccg cgtcgacccc
     1501 ggccagttcg ccgtgccggt gccgctgccg ccgtcgccga cgcgcgcgca gctgctggcg
     1561 gcgttcggca acgatcccga ggcggtcgac ggcgcgttca aggtgccgac cctgcgcaac
     1621 gtcgagctga cgggcccgta cttccacaac ggcggccagg ccacgctcga ggaggtggtg
     1681 cggttctaca accgcggcgg cgaccgccgg cgcaccggca acggcggcgc caacacgacc
     1741 ggcttcggca cgaacccgag caacctcgac cccgacatcg agccgctggg gctcagcgcg
     1801 cagcagcagg ccgacctggt cgcattcctg aagtcgctga cggacgagcg cgtgcgctgg
     1861 gaggtggcgc cgttcgacca cccgcagctg gcggtcatgc agggccactc ggcgaacgtg
     1921 aatccgcggc tcggcgcgcc gatggcggcc gacctcggca ccgtcctgcc ggaggtcggg
     1981 cgcggcgggc gcgcggtgct cggcctgccg gcgctgcggc cgttcgccga ccagctgcac
     2041 tgatcgccgg cgcgccggcg aagccgccgg cagcctcgtt cgggccggag tcgaccatgc
     2101 gcgtgccgcg cgtccgcagg gacgccggcg cgcgcgcgtc gatccggccc gccgcggggc
     2161 cgtcagcgcg cgccgttgcg gacggcgggc gc
//
LOCUS       DASRDG010000355         4054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_55560, whole genome shotgun sequence.
ACCESSION   DASRDG010000355 DASRDG010000000
VERSION     DASRDG010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55560"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFA35_05875"
     CDS             complement(<1..696)
                     /locus_tag="VFA35_05875"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55736.1"
                     /translation="MAKAGTSPWDLTALFNAADPKAGIAERHLWVIRLVEWLHRAPLE
                     RNEAPTLTPFAAPRGDAGALGADRPAPQAPAEARSTPTPVLRLRYLLGVLERNDSHRL
                     RAAEMLRLFLREVDSAALLADFGFAPRTGLWAEFGIRLRARLLPLTPATSDLGELFAL
                     LFPRGQDIAWLNAIDGATLARLARVLEAGDAGPAHDWRGPFFEAVTFLASSVRAAGFS
                     AQLRQRMSPELLVD"
     gene            complement(723..1097)
                     /gene="apaG"
                     /locus_tag="VFA35_05880"
     CDS             complement(723..1097)
                     /gene="apaG"
                     /locus_tag="VFA35_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HZT55737.1"
                     /translation="MARPEFTTTVTVRHLPEQSDRDAGKYAFAYTISITNSGDITGQL
                     IARHWIITDANGHVEEVRGLAVVGHQPVLKPGESFEYTSWTRLDTPHGTMRGTFLCMT
                     EDAQPFDAPIGEFGLTLASALH"
     gene            1158..1838
                     /gene="rpe"
                     /locus_tag="VFA35_05885"
     CDS             1158..1838
                     /gene="rpe"
                     /locus_tag="VFA35_05885"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZT55738.1"
                     /translation="MASPLIAPSILSADFARLGEEVRDVIAAGADWIHFDVMDNHYVP
                     NLTIGPMVCQAIRPHAARADGKPVPIDVHLMVEPVDAIAEAFAKAGADLISFHPEASA
                     HVDRTLQLIRSLGCKAGLVFNPATPIDVLDWVIDKVDLVLVMSVNPGFGGQSFIDSAL
                     RKLEQVRKRIDASGRDIRLEVDGGIKVDNIRAAADAGCDTFVAGSAIFGAKDYRAVIA
                     AMRARLAG"
     gene            1860..2882
                     /locus_tag="VFA35_05890"
     CDS             1860..2882
                     /locus_tag="VFA35_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZT55739.1"
                     /translation="MSRPGHDHHRHDGPAHEGAHHDGHAPGGHAPGGHAHGPARHDRA
                     FAIGIALNVAFVVVEAAAGWRIDSLALLADAGHNLGDVLGLVLAWAGAFAGRLAPDDR
                     HTWGWKRASILAAFANALLLLVAMGSLVWAAVLRLQSPEATQPGVIMVVAAIGIVVNL
                     GTALLFMRGRHDDLNIRGAFLHMSADAAVSAGVVVAGALVLWQGWLWADPVTGLLIAA
                     VILVGTWQLFRDSLHLMFDGVPSSIDLDAVRAELEALPGVDCVHDLHVWATGTTDVVL
                     TAHVVMPAGHPDDAFFRAAAARMLERFAIGHVTLQASTEPLMAACAGPKAPPSAAREP
                     ILSDSH"
     gene            2890..3888
                     /locus_tag="VFA35_05895"
     CDS             2890..3888
                     /locus_tag="VFA35_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZT55740.1"
                     /translation="MKYKDYYDTLGVPRSATQDDIKKAYRKLARKYHPDVSKAADAEH
                     RFKEVNEANEVLRDPEKRAAYDQMGSNWKAGQEFQPPPNWDAGFEFRSGGPGGGFGGG
                     SPFGADFDPSDFFESLFGRGARGAGGGASARRSVSTQGEDHRAKALIDLEDAYHGAER
                     SIVLRVPKETADGRVVLEERRLDVHIPRGIRPGQQLRLAKQGAPGSNGGPPGDLYLEI
                     AFNPHPLFKVEGADVHVELPVAPWEATLGATVDAPTPEGTVQLTVPKGSAQGRKLRLK
                     ARGLPATRAGGTAGDLYVVLTIALPPADSGKAREAYEGLAKAFPGYDPRASLAGGA"
     gene            complement(3871..>4054)
                     /locus_tag="VFA35_05900"
     CDS             complement(3871..>4054)
                     /locus_tag="VFA35_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55741.1"
                     /translation="YERFLHETAFRLPGVTHIRSSVVLKEVKAEAGVPIPGTGQASAG
                     RLRKPAGRQRASRAAG"
BASE COUNT          587 a         1461 c         1437 g          569 t
ORIGIN      
        1 gtcgaccagc agctccgggc tcattcgctg gcgcagctgg gcggagaagc ccgcggcgcg
       61 caccgagctg gcgaggaagg tgacggcttc gaagaacggg ccgcgccagt cgtgcgccgg
      121 accggcatcg ccggcttcga ggacgcgcgc cagccgcgcc agcgtggcgc cgtcgatcgc
      181 gttcagccag gcgatgtcct ggccgcgcgg aaagagcagg gcgaacagct cgccgaggtc
      241 gctcgtcgcc ggcgtcagcg gcagcagccg ggcgcgcagc cggatgccga actcggccca
      301 caggcccgtg cgcggggcga agccgaaatc ggcgagcagc gctgcgctgt cgacctcgcg
      361 caggaacagg cgcagcattt ctgcggcgcg cagccgatgc gagtcgttcc gctcgagcac
      421 gcccaggaga tagcgcaagc gcagcaccgg cgtcggcgta ctgcgcgcct cggcgggcgc
      481 ctgcggcgcc ggccggtccg ccccaagggc gccggcgtcc ccgcgggggg cagcgaacgg
      541 agtgagcgtg ggggcctcat tcctttcgag cggggcgcgg tgcagccact cgaccaggcg
      601 gatcacccac agatggcgct cggcgatgcc ggccttcgga tcggccgcgt tgaacagcgc
      661 cgtcaggtcc cagggcgagg ttccggcttt cgccatcgcg acgcgcaggc gcgggcccgc
      721 ggctagtgca gggcagaggc gagcgtcagg ccgaactcgc cgatcggcgc gtcgaacggc
      781 tgcgcgtcct cggtcatgca caggaaggtg ccgcgcatcg tgccgtgcgg cgtgtccagg
      841 cgcgtccagc tcgtgtactc gaagctctcg cccggcttga gcaccggctg gtgaccgacc
      901 accgccaggc cgcgcacttc ctcgacgtgg ccgttggcgt cggtgatgat ccagtggcgg
      961 gcgatcagct ggccggtgat gtcgccgctg ttggtgatgg agatggtgta ggcgaaggcg
     1021 tacttgcccg cgtcccggtc cgattgctcg ggcaggtgac gaactgtcac ggtggtcgtg
     1081 aactcgggtc tggccatgcg ccgattctcg caggcatgcg cgaagacccc gacctagaat
     1141 cgatcgacag gtaccgcatg gccagccccc tcatcgcccc cagcattctt tcggccgatt
     1201 tcgcgcggct cggcgaggaa gtgagggacg tcatcgccgc cggcgccgac tggatccact
     1261 tcgacgtgat ggacaaccac tacgtcccga atctgacgat cggcccgatg gtctgccagg
     1321 cgattcggcc gcacgctgcc cgggccgacg gcaagccggt gccgatcgac gtgcacctga
     1381 tggtcgagcc ggtcgacgcg atcgccgagg ccttcgccaa ggccggcgcc gacctcatca
     1441 gctttcatcc cgaggcgagc gcgcacgtcg atcgcacgct gcagctgatc aggagcctgg
     1501 gctgcaaggc cgggctggtg ttcaacccgg cgacgccgat cgacgtgctc gactgggtca
     1561 tcgacaaggt cgacctggtg ctggtgatga gcgtcaaccc cggcttcggc ggacagagct
     1621 tcatcgactc ggcgctgcgc aagctcgagc aggtgcgaaa gcgcatcgac gccagcggcc
     1681 gcgacatccg gctcgaggtc gatggcggca tcaaggtcga caacatccgc gccgccgccg
     1741 acgccggttg cgacaccttc gtcgccggca gcgcgatctt cggggccaag gactaccgcg
     1801 cggtgatcgc cgcgatgcgc gcccgcctgg ccggctgaag cattgagcag gcgctcgcca
     1861 tgagccgccc cggccacgat catcaccggc acgacggccc ggcgcacgag ggtgcgcatc
     1921 acgacggcca cgcacccggc gggcacgccc cgggcggcca cgcccacggt cccgcccgcc
     1981 acgaccgcgc cttcgcgatc ggcatcgccc tcaacgtcgc cttcgtcgtc gtcgaggcgg
     2041 ctgccggctg gcgcatcgat tcgctcgcgc tgctcgccga tgccggccac aacctcggcg
     2101 acgtgctcgg cctggtgctg gcctgggccg gcgcgttcgc cggccggctc gcgcccgacg
     2161 accggcacac ctggggctgg aagcgggcct cgatcctggc cgcgttcgcc aatgcgctgc
     2221 tgctgctggt ggcgatgggc tcgctggtct gggccgcggt gctgcgcctg cagtcgccgg
     2281 aggcgacgca gcccggcgtc atcatggtcg tggcggcgat cggcatcgtc gtcaacctcg
     2341 gcaccgcgct gctgttcatg cgcggccgcc acgacgacct caatatccgc ggcgccttcc
     2401 tccacatgtc ggccgacgcc gcggtctcgg ccggcgtcgt cgtcgccggt gcgctggtgc
     2461 tgtggcaagg gtggctgtgg gccgacccgg tgaccgggct gctgatcgct gcggtgatcc
     2521 tggtcggcac ctggcagctc ttccgcgatt ccctgcacct catgttcgac ggcgtgccga
     2581 gctcgatcga cctcgacgcg gtgcgcgccg agctcgaggc gctgcccggc gtcgactgcg
     2641 tccacgacct gcacgtctgg gcgaccggca cgaccgacgt cgtgctcacg gcccacgtcg
     2701 tcatgccggc cggccatccg gacgatgcgt tctttcgcgc cgccgcggcg cgcatgctgg
     2761 agcgcttcgc gatcggtcac gtcaccctgc aagcctcgac cgagccgctg atggcggctt
     2821 gcgccgggcc gaaggcgccc ccatctgcgg ctcgcgagcc catcctcagc gactcgcatt
     2881 gactgaccga tgaaatacaa ggactactac gacaccctgg gcgtgccgcg ctcggccacc
     2941 caggacgaca tcaagaaggc ctaccgcaag ctcgcccgca agtaccaccc ggacgtgagc
     3001 aaggccgccg acgccgagca tcgcttcaag gaggtcaacg aagccaacga ggtcctgagg
     3061 gatccggaga agcgcgccgc ctacgatcag atgggcagca actggaaggc cgggcaggaa
     3121 ttccagccgc ctccgaactg ggacgcaggc ttcgagttcc gcagcggcgg gccgggcggc
     3181 ggcttcggcg gcggcagccc cttcggcgcc gacttcgacc cgagcgattt cttcgagtcg
     3241 ctgttcggcc gcggtgcgcg cggcgccggc ggcggcgcat cagcgcggcg cagcgtcagc
     3301 acccagggcg aggatcaccg cgccaaggcg ctgatcgacc tcgaggacgc ctaccacggc
     3361 gccgagcgca gcatcgtcct gcgcgtgccg aaggaaacgg ccgacggtcg tgtcgtcctc
     3421 gaggagcgcc ggctcgacgt ccacatcccg cgcggcatcc gccccggcca gcaactgcgc
     3481 ctggccaagc agggcgcgcc cggcagcaac ggcgggccgc cgggcgacct gtacctcgag
     3541 atcgcgttca atccgcatcc gctgttcaag gtcgagggcg ccgatgtcca cgtcgagctg
     3601 ccggtagcgc cctgggaagc gacgctcggc gcgaccgtgg atgcgcccac gcccgagggc
     3661 acggtgcagc tcaccgtccc caagggctcg gcccagggac gcaagctccg tctgaaggcg
     3721 cgcggcctgc cggccacgcg ggccggcggc accgcgggcg acctctacgt ggtgctgacg
     3781 atcgccctgc cgccggccga ttccggcaag gcgcgcgagg cctacgaggg cctggccaag
     3841 gcgttccccg gctacgatcc gcgggcttcg ctagccggcg gcgcgtgaag cgcgctgccg
     3901 gccggcgggc ttgcgcagtc ggcccgcgct tgcctggccc gtacccggaa tcggcacgcc
     3961 ggcctcagcc ttgacctcct tgagcaccac gctcgagcgg atgtgggtca cgccgggcag
     4021 gcggaatgcc gtctcgtgca ggaagcgctc gtag
//
LOCUS       DASRDG010000356         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_167620, whole genome shotgun sequence.
ACCESSION   DASRDG010000356 DASRDG010000000
VERSION     DASRDG010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167620"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFA35_05905"
     CDS             complement(<1..465)
                     /locus_tag="VFA35_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZT55742.1"
                     /translation="MGSDMNVNEGLRSRALHSRRRWLAHCTALALCSTAALDAPARAQ
                     GVSKAATVGGPLVLVVGDSLSAEYGLARGAGWVALLDARLAQQKIAARVVNASISGDT
                     TSGGRTRLPALLTQHKPGIVVIELGGNDALRGLPLEMTRDNLTVMTQAAKAAG"
     gene            452..1180
                     /locus_tag="VFA35_05910"
     CDS             452..1180
                     /locus_tag="VFA35_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT55743.1"
                     /translation="MSEPIIAVERVTKQVQDSTGTLTILHEIDFSLAPRQSAAIVGAS
                     GSGKSTLLAIIAGLDTPTTGTVRLAGTDLFTIDEDARAAVRAAKVGFVFQSFQLLGNL
                     NALENVMLPLELQGRNDARVLATEMLKRVGLGERLRHYPKLLSGGEQQRVALARAFVV
                     QPAVLLADEPTGSLDFATGETVMELMFALNREAGTTLVLVTHDPDIAARCDRQLRIEA
                     GRVEREAKDEGPRLRGSASAALGE"
     gene            complement(1212..2030)
                     /locus_tag="VFA35_05915"
     CDS             complement(1212..2030)
                     /locus_tag="VFA35_05915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZT55744.1"
                     /translation="MKKTYFAAGAAMLLSVMLALPLAAQAQNIAIVNGKAVPKARVDA
                     ILKQVQTQAAAQNQQLPPDLDQRVRDSVVLGEIFSQEAEKRGLAATAEYKSQMEQARQ
                     QVLTSALFADFAKAHPVADAEVKAEYEKFKAQATGMEYRARHILVEKEDEAKALIAQI
                     KAGAKFEDLAKKNSKDPGSGANGGDLDFAAANAYVPEFSQAMVKLKKGEMTQEPVKTQ
                     FGYHIIRLDDTREAQFPPFDDVKAQLKQRMEQQKLAAYRDEIRVKAKTDYKFSN"
     gene            complement(2054..2323)
                     /locus_tag="VFA35_05920"
     CDS             complement(2054..2323)
                     /locus_tag="VFA35_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HZT55745.1"
                     /translation="MSVAAPEIELALTSALQPLKLAVEDDSHLHAGHAGAREGRHFTV
                     RVTSARFDGLSRVARHRLIYDALQLLIPRGIHALAIEARAPGESE"
     gene            complement(2320..>2782)
                     /locus_tag="VFA35_05925"
     CDS             complement(2320..>2782)
                     /locus_tag="VFA35_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HZT55746.1"
                     /translation="VQIVVLLIRRQKIDLMLWMTFVLVVVLGGATVWFHNATFIKWKP
                     SVLYWAMGLAFWISQAFFRKNLLQTLIGGQLALPAPVWQRLNFAWIAFFGLLGLLNLY
                     VAYTYSDSTWFTFKAFGVTGLMLVFMFAQGFYISKHLAPEADAGKARDHKP"
BASE COUNT          421 a          940 c          938 g          483 t
ORIGIN      
        1 gccggcggcc ttcgcggcct gcgtcatcac ggtgaggttg tctcgcgtca tctccagcgg
       61 cagcccgcgc agcgcgtcgt tgccgccgag ttcgatcacg acgatgccgg gcttgtgctg
      121 cgtcaggaga gccggcaatc gcgtgcggcc gcccgacgtg gtgtcgccgc tgatgctggc
      181 gttgacgacg cgcgcagcga tcttctgttg cgccagccgc gcgtcgagca gcgcgaccca
      241 cccggcaccg cgcgcgagcc cgtactcggc cgacaggctg tcgccgacga cgaggaccaa
      301 cgggccgccc actgtggcgg ccttgctcac cccctgcgcg cgcgccggcg cgtcgagtgc
      361 cgcggtgctg cacagggcca acgcggtaca gtgcgccagc catcggcgcc tcgagtgcag
      421 cgcccgtgat cgaagtccct cattcacatt catgtccgaa cccatcattg ccgtcgagcg
      481 cgtcaccaag caggtccagg actccaccgg cacgctgacc attctccatg agatcgactt
      541 ttcactggcg ccgcgccagt cggcggcgat cgtcggcgcg tcgggctcgg gcaagagcac
      601 cttgctggcc atcatcgccg ggctggacac gccgaccacg ggcaccgtgc ggctcgccgg
      661 cactgatctg ttcacgatcg acgaagacgc gcgcgccgcg gtgcgtgccg cgaaggtggg
      721 gttcgtgttc cagagtttcc agttgctcgg caacctgaac gcgctcgaga acgtgatgct
      781 gccgctcgaa ctgcagggcc gcaacgacgc gcgcgtgctc gcgaccgaga tgctcaagcg
      841 cgtcggcctc ggcgagcgcc tgcggcacta cccgaagctg ctctcgggcg gtgagcagca
      901 gcgcgttgcg ctggcgcggg cgttcgtcgt gcagccggcc gtgctgctcg ccgacgagcc
      961 gaccggcagc ctcgacttcg cgaccggcga gaccgtgatg gaactgatgt tcgcgctcaa
     1021 ccgcgaggcc ggcacgacgc tggtgctggt cacgcacgac cccgacatcg cggcacgctg
     1081 cgatcggcag ttgcgcatcg aagccggtcg cgtagagcgc gaagcgaagg acgaggggcc
     1141 ccgtttacgg ggatcggcca gcgcagcgct cggcgagtga agcgggctcg aagcccgctt
     1201 cgcgagcgcg ctcagttgct aaatttgtag tcggtcttgg ccttcacgcg gatctcgtcg
     1261 cggtacgccg ccagcttctg ctgctccatg cgctgcttca gctgcgcctt gacgtcatcg
     1321 aacggcggga actgggcctc gcgcgtgtcg tcgaggcgga tgatgtggta gccgaactgg
     1381 gtcttcaccg gctcctgcgt catctcgcct ttcttcagct tcaccatcgc ctgcgagaac
     1441 tcgggcacat aggcgttcgc ggcggcgaaa tcgaggtcgc cgccgttggc gcccgatccc
     1501 gggtccttcg agttcttctt cgccaggtcc tcgaacttgg cgccggcctt gatctgcgcg
     1561 atcagcgcct tggcctcgtc ttccttctcg accaggatat ggcgcgcgcg gtactccatg
     1621 cccgtggcct gcgccttgaa cttctcgtac tcggctttga cctcggcgtc ggcgaccgga
     1681 tgcgccttgg cgaagtcggc gaacagcgcg ctcgtcagca cctgctggcg cgcttgttcc
     1741 atctggctct tgtactccgc cgtcgcggcc aggccgcgtt tttcggcctc ctgcgagaag
     1801 atctcgccga gcacgaccga gtcgcgcacg cgctggtcga ggtcgggcgg gagttgctgg
     1861 ttctgcgcgg cggcctgggt ctgcacctgc ttgaggatcg cgtcgacccg tgccttgggc
     1921 acggccttgc cgttgacgat cgcgatgttt tgcgcctgcg ccgccagcgg caacgcgagc
     1981 atcacggaca gcagcatggc ggcgcccgca gcgaaatagg tcttcttcat gagcatgggg
     2041 gaagttgggg gtttcactcc gattcgccgg gcgcgcgcgc ctcgatggcc aaggcgtgga
     2101 taccgcgcgg gatcaggagt tgcagcgcat catatatgag ccggtgccgc gccacgcgtg
     2161 acaaaccgtc gaagcgcgcc gacgtgaccc gcaccgtgaa atgacggccc tcgcgtgcgc
     2221 cggcgtggcc ggcgtgcagg tggctgtcat cctcgaccgc gagcttcagc ggctgcagcg
     2281 cgctcgtcag cgcgagttcg atctcgggcg cggcaacgct cacggcttgt ggtcccgcgc
     2341 cttgccggcg tcggcctcgg gtgccaggtg cttgctgatg tagaagccct gggcgaacat
     2401 gaacaccagc atcaggccgg tcacgccgaa ggccttgaag gtgaaccagg tcgagtcgga
     2461 gtaggtgtag gccacgtaca ggttcagcag cccgagcagg ccgaagaacg cgatccacgc
     2521 gaagttcagg cgttgccaga ccggcgcggg cagcgcgagc tggccgccga tcagcgtttg
     2581 cagcaggttc ttgcggaaga acgcctggct gatccagaac gccagcccca tcgcccagta
     2641 cagcacgctg ggcttccatt tgatgaaggt cgcgttgtgg aaccagaccg tcgcgccgcc
     2701 gaggacgacg acgagcacga aggtcatcca cagcatcagg tcgatcttct ggcgccggat
     2761 cagcagcacc acgatctgca cc
//
LOCUS       DASRDG010000357         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1660069, whole genome shotgun sequence.
ACCESSION   DASRDG010000357 DASRDG010000000
VERSION     DASRDG010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660069"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFA35_05930"
     CDS             <1..920
                     /locus_tag="VFA35_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZT55747.1"
                     /translation="WDDELLALLEVPREVLPEVHPSSHAYGHTRADLLGAPIPIGGVA
                     GDQQAALFGQACFKAGLAKNTYGTGCFMLMHTGARFLTSTNGLITTAAAQLGTTAAAA
                     PEFALEGSVFIGGAVVQWLRDGLGAIRASAEVQALAESVPDSGGVMFVPAFTGLGAPY
                     WNADARGAIVGLSRGSTVAHIARAALESIAFQSAALLQAMARDVTAAGGRTLTELRVD
                     GGASVNDLLMQFQADLLGIPVVRPEVVETTALGAAYLAGLATGVYRGLDELSAAWRVG
                     RRFEPMLARARADALMQGWDHAVRQTLAP"
     gene            926..1690
                     /locus_tag="VFA35_05935"
     CDS             926..1690
                     /locus_tag="VFA35_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZT55748.1"
                     /translation="MAGWETYLAFVGCVAIANFVQNLTGFAFGLILLGLVSVFDLASV
                     ADAANAATVLTLVNAASYFRLHRLRPEWRIVRPAILPSLVGVGVGVAMLTWLSGNATQ
                     ALRGLLGLAILACAFGLLVDSKPRATLSRPAAFAAVGAISGLMGGLFSSAGPPLVYHM
                     YRQPAPREVIQQCLILMFAINQVLRLALVVGSGRFSLHSGWLCAAAVPAVYVVTWLQH
                     RYMPPVGIVATRRVVSGLLIVAGGSLLVSVLGVIMS"
     gene            complement(1766..>2013)
                     /locus_tag="VFA35_05940"
     CDS             complement(1766..>2013)
                     /locus_tag="VFA35_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZT55749.1"
                     /translation="LTSAFEPDRERLRRAIAATFARRGTAIPSLVPDGLSDDFAEDPG
                     KLRQWAAFARNLSGPIPELSATVAQLRDRLAPYLAST"
BASE COUNT          250 a          679 c          728 g          356 t
ORIGIN      
        1 cctgggacga cgagctgctc gcgctgctcg aggtgccgcg cgaggtgctg cccgaggtgc
       61 atccgtcgag ccacgcctat ggccacacgc gggccgacct gctcggcgcg ccgatcccga
      121 tcggcggtgt cgcgggcgac cagcaggccg cgctgttcgg ccaggcctgc ttcaaggcgg
      181 gcctggcgaa gaacacctat ggcacgggct gcttcatgct gatgcacacc ggcgcgcggt
      241 tcctgacgtc gaccaacggg ttgatcacga ccgccgctgc gcagctcggc acgaccgccg
      301 ctgccgcacc cgagttcgcg ctcgaaggca gcgtcttcat cggcggcgcg gtcgtgcagt
      361 ggctgcgcga cgggctcggc gcgatccgcg ccagcgcgga ggttcaggcg ctcgccgaga
      421 gcgtgcccga cagcggcggc gtgatgttcg tgcccgcctt caccggcctg ggcgcgccgt
      481 actggaacgc cgacgcgcgc ggcgcgatcg tcgggctgag ccgcggcagc acggtcgcgc
      541 acatcgcgcg cgccgcgctc gagagcatcg cgttccagag cgccgcgctg ctgcaggcga
      601 tggcgcgcga cgtcactgcg gcgggcgggc ggacgctgac cgagctgcgc gtcgacggcg
      661 gcgcgtcggt caacgacctg ctgatgcagt tccaggccga cctgctgggc attccggtcg
      721 tgcgtcccga agtcgtcgaa acgacggcgc tcggcgccgc gtaccttgcg gggcttgcca
      781 ccggcgtcta ccgtggcctc gacgaactca gcgcggcgtg gcgcgtcggc cggcgctttg
      841 agcccatgct ggcgcgcgcg cgcgccgacg ctctcatgca aggctgggac cacgccgtgc
      901 gccagaccct cgcaccgtga cgcccatggc cggctgggag acctacctgg cgttcgtcgg
      961 ctgcgtggcg atcgccaact tcgtccagaa cctgaccggc ttcgccttcg gcctgatcct
     1021 gctcgggctc gtcagcgttt tcgatctcgc gtcggtggcg gatgccgcca atgcggcgac
     1081 ggtgctgacg ctggtcaacg cggcgtcgta cttccgcctg catcggcttc gcccggagtg
     1141 gcgcatcgtg cgcccggcga ttctgccgag tctcgtcggc gtcggtgtcg gtgtcgcgat
     1201 gctcacatgg ctcagcggca atgcgacgca ggcgctgcgc ggcctgctgg gcctggccat
     1261 cctcgcgtgc gcgttcgggc tgctcgtcga ctccaagccg cgggcaacgc tgtcgaggcc
     1321 tgcggcgttc gcggcggtcg gcgcgatctc ggggctgatg ggtggactgt tttccagcgc
     1381 cgggccaccg ctggtctatc acatgtaccg ccagccggca ccgcgcgaag tcatccagca
     1441 gtgtctgatc ctgatgttcg cgatcaacca ggtcctgcgg ctcgccttgg tcgtcggctc
     1501 gggcaggttc tcactgcatt cggggtggct gtgtgcggcg gccgttcctg cggtctatgt
     1561 cgtgacctgg ctgcagcatc ggtacatgcc tcccgtcggg atcgtggcga cgcgtcgcgt
     1621 cgtcagcggc ctgctgatcg tggcgggcgg ctcgctgctg gtctcggtgc tgggcgtgat
     1681 catgtcgtaa ccgaaccggc aacgtgtcac ggtagtcgtt cttaggtgat ctcgtttcag
     1741 ggcggcgccc ggtgtgtatc tcgtgtcagg tcgaggcgag gtagggggcg agcctgtctc
     1801 gtagctgagc gaccgtggcg ctgagctcag gaattgggcc cgagaggttc cgcgcaaacg
     1861 ccgcccactg tcgcagcttg cctggatctt cagcgaagtc gtcgctcagg ccatctggaa
     1921 ccagcgaagg aatcgcggtg ccgcgacgag cgaacgttgc tgctatggcc cggcgcaggc
     1981 gctcgcggtc cggttcgaat gcggatgtga gca
//
LOCUS       DASRDG010000358         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1437188, whole genome shotgun sequence.
ACCESSION   DASRDG010000358 DASRDG010000000
VERSION     DASRDG010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437188"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..970
                     /locus_tag="VFA35_05945"
                     /pseudo
     CDS             152..970
                     /locus_tag="VFA35_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1400..1579)
                     /locus_tag="VFA35_05950"
     CDS             complement(1400..1579)
                     /locus_tag="VFA35_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55750.1"
                     /translation="MLQLQHHAPVARRHVVDQLEHPRRLRLAQHRLNVRAFGLGDPQP
                     TGQSFAVPHGHYRIN"
     gene            complement(1743..1819)
                     /locus_tag="VFA35_05955"
     tRNA            complement(1743..1819)
                     /locus_tag="VFA35_05955"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1783..1785),aa:Met,seq:cat)
     gene            complement(1864..>3146)
                     /gene="dsbD"
                     /locus_tag="VFA35_05960"
     CDS             complement(1864..>3146)
                     /gene="dsbD"
                     /locus_tag="VFA35_05960"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HZT55751.1"
                     /translation="GIDGALRSGKLWTVVGVFLVAGVLLSLTPCVLPMLPILSSIIVG
                     QGATVSRGRGFALALSYSLGMALVYTALGVAAGLAGEGLAAALQQPWVLALFAAGLVA
                     LSLSMFGVYELQLPSVFTSRLSNASQRLPGGRFAGVFAMGGVSALIVSPCVAAPLAGA
                     LVYISQTRDVALGGTALFALAAGMSVPLLLMGASAGALLPRAGGWMDEVKRCFGVLLL
                     GVALWTVQSILPAALALALWGALALGAAVMLASSQHGHHRSGWTKSLWRRVVAAVLAV
                     CGLMQLVGAASGGTNPLQPLAHLRASASAAARGPVFTTVRSVAELDNALRTAGRPVML
                     DFYADWCVSCKEMERFTFVDPLVQKKMAGALLLKADVTANSDADRALLKRFNLFGPPG
                     TIFFDPQGRELPAARIAGFQDATRFLQTLQTAGL"
BASE COUNT          561 a         1061 c         1125 g          399 t
ORIGIN      
        1 actttcggtc agagaacccg tcgccgccgt agcatgagca gtccacccgc cctcaatgcg
       61 cgagcgctgg cgcgctcgcg cctcatcacg ctgcttgcgg agcggctggt acgcgaggcc
      121 cagacttcca ccgtcaccga acctctcctc gatggaaccg actacaccca cccgcgtggc
      181 gctctacgcg cggttcagca ccgaccttca gcgccagaca tcgataggtg accaactcgt
      241 tgcagcgcgc gagcgcgccg cgcgtgaggg ctggcctgtc gtcatcgagt gcgccgacga
      301 aggagtgagc tgctctgtgc cggtggcgat gcgcgccggc ggaaaggcgc tgcttgccga
      361 cgcgctcgcg cggcgcttcg aggtgttgat cgtcgaaggc ctcgaccgat tgagccgcga
      421 catgggcgaa caggatcagg tcgtcaagcg actcgaatat cggggcatcc ggctgatcgg
      481 caccagcgac ggctacgaca cgcaggccaa gggccgcaag gtgatgcggg tagcgcgcgg
      541 cttggtcaac gagctgtacc tcgacgatct gcgcgacaag acgcatcgag ggctcgccgg
      601 tcagttcgcg cgcggcttga gcgccggtgg ccgcagcttc ggctatcgca ccgaggatgc
      661 gggcagcgcg ggaggccggc ggctggtaat cgaggcaacc gaagcggccc atgtccgatg
      721 gatattcgag caagcagcgg ccgggcacag cgtcaggtcg atcgctcacc agctcaacgc
      781 gcgaggcgtg cccagccccc gcggtggtac gtgggccgtc agcgcgctgt tcggaagcgc
      841 agcgcggggg ctgggcctgc tcaacaacga gatgtacatc ggccgggtga tctggaaccg
      901 gcggcagtgg gtcaaagacc cggagaccgg caaccggcga tacatcgagc gcccgaagag
      961 cgaatggcag gaacgcgatg cgcccgagct gcgaatcatc gacgcggacc tatggcagcg
     1021 cgcccacggc cgcgaaaggc gcgggccggc gccaggtacg gggcgccacc gcggcgcttc
     1081 gccgaagacg ctcttcggcg gcctgctgac gtgccacgca tgcggtggcc cgatcatcgc
     1141 gatcaaccgc gctcgctacg gctgcagcgt gcacaaggat cgaggaaaca gcgtttgcag
     1201 cagcggcgcg agcttcctgc gcgatgtggt ggaccgccga ctcgtggctg agctgcgcga
     1261 cgagttgctg caccccggcg cactcgccga gctgcacgct gcggtacggg tcatcgccga
     1321 cgcgcagcag cgcgaggccg gcagcgggca cgaggccgca cgcaagcggc tcaagcaggt
     1381 tcacggcgag atagggcgcc tagttgatgc ggtagtggcc gtggggcaca gcgaagctct
     1441 gaccggtcgg ctgcgggtcg ccgaggccga acgctcggac attgaggcga tgttgtgcga
     1501 gccgaagccg ccgggggtgc tcaagctgat cgacgacgtg acggcgcgct accggcgcat
     1561 ggtgctgcag ttgcagcacg tcttgaacga tgaaggcgac cgccagcgaa cgcgctcgat
     1621 cttggccgac atgctcgggc cggtgacgct gctgagagat cgggagaccg gcgagaattt
     1681 cgccgaactt gatgagcccg ccgagcggct gctggtacag gccgtcggcg agtctctgaa
     1741 agtggttgcg agggcaggat ttgaacctgc gacctttggg ttatgagccc aacgagctac
     1801 cagactgctc cacctcgcga ctgagcctgc gactatagca cagctcgcga gttcccgcgg
     1861 ggctcacagg ccggcggtct gcaatgtctg caggaagcgg gtcgcgtcct ggaagccggc
     1921 gatgcgcgca gccggcagtt cgcggccctg cgggtcgaag aagatcgtgc cgggcgggcc
     1981 gaacaggttg aagcgcttca gcagcgcgcg gtcggcgtcg ctgttcgcgg tcacgtcggc
     2041 cttgagcagc agcgcgccgg ccatcttctt ctgcacgagg gggtcgacga aggtgaagcg
     2101 ctccatctcc ttgcacgaga cgcaccagtc ggcatagaag tcgagcatca ccgggcggcc
     2161 cgcagtgcgc aacgcgttgt cgagttcggc aacgctgcgc acggtcgtga acaccgggcc
     2221 gcgggcggcc gccgacgcgc tggcgcgcag gtgcgcaagc ggctgcaacg ggttcgtgcc
     2281 gcccgacgcg gcaccgacga gctgcatcag gccgcacacc gccagcacgg ccgcgaccac
     2341 gcggcgccag aggctcttgg tccaaccgct gcggtggtgg ccgtgctgcg acgaggcgag
     2401 catcaccgcc gcgccgagcg cgagcgcgcc ccagagcgcg agcgccagcg ccgcgggcag
     2461 tatcgattgc acggtccaca gcgccacgcc gagcagcagg acgccgaagc agcgcttgac
     2521 ctcgtccatc cagccgcccg cgcgcggcag cagcgcgccg gccgaggcgc ccatcaacag
     2581 cagcggcacg ctcatgccgg cggcgagcgc gaacagcgcg gtcccgccga gcgccacgtc
     2641 acgggtctgg ctgatgtaga ccagcgcacc ggccagcggc gctgcgacgc acgggctgac
     2701 gatcagcgcc gagacgccgc ccatcgcgaa cacgccggcg aaacgcccgc ccggcaggcg
     2761 ctgcgacgcg ttcgacaggc ggctggtgaa caccgacggc agctgcagtt cgtagacgcc
     2821 gaacatcgac agcgacaacg ccacgagccc ggccgcgaac agcgcgagca cccagggctg
     2881 ctgcaacgcc gcggccaggc cctcaccggc caggccggcg gccacgccga gggcggtata
     2941 gacgagcgcc atgccgagcg aataactgag cgcgagcgcg aagccgcgcc cacgcgagac
     3001 ggtggcgccc tggccgacaa tgatcgacga caggatcggc agcatcggca gcacgcacgg
     3061 cgtgagcgac agcagcacgc cggcaacaag gaacacgccg acgacggtcc agagcttgcc
     3121 gctgcgcagc gcaccgtcga tgccgg
//
LOCUS       DASRDG010000359         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_414874, whole genome shotgun sequence.
ACCESSION   DASRDG010000359 DASRDG010000000
VERSION     DASRDG010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414874"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFA35_05965"
     CDS             <1..484
                     /locus_tag="VFA35_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZT55752.1"
                     /translation="TMSDRVAVFNSGVIQQIDAADRMYEAPANRFVAGFIGDGTMLRG
                     TLRAAHAGGHCEMALADGRVVGGVNVNAAAVGAEVVAGIRPERIVVHTQAAPARANVL
                     QAQVRGIIYFGDHLRLLCGVGEGQDDATVKLPLSSAAMPQVGDTLWLELPHELTRIYI
                     "
     gene            528..1556
                     /locus_tag="VFA35_05970"
     CDS             528..1556
                     /locus_tag="VFA35_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT55753.1"
                     /translation="MKIHTLVVTLAAAFALPALAQQQITVVNFGGANANAQKKAYYEP
                     FEKTGTKVVAVEYNGEQAKIKVMVETKKVTWDVVEVESPDVARGCDEGLFEKIDYSKV
                     APKADFLPAAVTECGVGVFVWSTVMAWNGDKLKDGPKTWADFWDTKKFPGKRGMRKGA
                     RYNLEFALMADGVKPADVYKVLATKEGAERAFKKLGELKSSIQWWEAGAQPPQFLVAG
                     DVVMTTVYNGRIDAANREGKNLKIGWTGGIYDLDYWVIPKGAPNKAASLKFIAFASTP
                     EAQAEYAKNIAYGPTNTKALAKLDAKVLANLPTSPANSKDALQFGLKFWADQGEDLEK
                     RFASWATN"
     gene            1582..2850
                     /locus_tag="VFA35_05975"
     CDS             1582..2850
                     /locus_tag="VFA35_05975"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55754.1"
                     /translation="MNASITAPLPGPADPLGLRGQLARAERRRKWRAFSLTLPLLLFL
                     LLTFLVPIAALLKRAIENPEVANALPRTVVALQGWKPADVPEPAAFAAITQDLGALTD
                     SSDAGALARRLNSETPGARSLIMATFKALPLEGTSATSPAADIKTALLALDARWGETK
                     YWQAIAKNGSRWTPDYLLTSVDLRRDAQGAIERVDPAQRVFGGILVRTFEISAIVTLW
                     CLLLGYPLAYWLSTLSARQANVLMILVLVPFWTSILVRVAAWIVLLQSEGLVNHALID
                     LHLIDKPLALLFNRTGVIISMVHILLPFMILPLYSVMTSVPPTYLRAAVSLGSPPLAA
                     FFRVYVPQTYPGIGAGALLVFILSIGYYVTPALLGGPTDQMLSYYIAQYTNVTVNWGM
                     ACALGALLLAATLVLYAIYRRVTKSELSLG"
     gene            2855..3685
                     /locus_tag="VFA35_05980"
     CDS             2855..3685
                     /locus_tag="VFA35_05980"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55755.1"
                     /translation="MSALSKLLPRFPAYSTPLDKLGWWALRFVCVGVLVFLLLPILVI
                     IPLSFSDSSFLVYPIPGWSLKWYDNLFGSAEWARAAKNSFIIAPAATLLATVLGTLAA
                     VGLARTDFPFKGLLMSLLIAPMVVPIVVVGVSTYLFFAPLGMADSYLSLIIVHAALGA
                     PFVLTTVLATLQGFNQNLVRASLSLGESPLRTFFRITLPVIAPGVVSGALFAFATSFD
                     EVVVVLFLAGPEQLTLPRQMFNGIRENISPTIAAVATLLIIFTTSLLLALEWLRGRRR
                     "
     gene            complement(3708..>3922)
                     /locus_tag="VFA35_05985"
     CDS             complement(3708..>3922)
                     /locus_tag="VFA35_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55756.1"
                     /translation="ALMQPLRVGADDLTVATAIGIGQYPQDGAQPEALLRRAVGLAAA
                     AQAQGRAGFSNFGEGGAAPVSAANDE"
BASE COUNT          598 a         1407 c         1297 g          620 t
ORIGIN      
        1 gacgatgagc gaccgcgtcg cggtgttcaa cagcggcgtg atccagcaga tcgacgcggc
       61 cgaccgcatg tacgaagcgc cggcgaaccg cttcgtcgcc ggcttcatcg gcgacggcac
      121 gatgctgcgc ggcacgctgc gcgcggcgca cgcgggcggg cactgcgaga tggcgctcgc
      181 cgacggccgc gtggtcggcg gcgtgaacgt caacgccgcg gcggtcggcg ccgaagtcgt
      241 ggccggcatc cggcccgagc gcatcgtcgt gcacacccag gccgcgccgg cgcgcgccaa
      301 cgtgctgcag gcgcaggtgc gcggcatcat ctacttcggc gaccacctgc ggctgctgtg
      361 tggcgtgggc gaaggccagg acgacgccac cgtgaagctg ccgctgtcgt cggccgcgat
      421 gccacaggtg ggcgacacgc tctggctcga gttgccgcac gagctgaccc gcatctacat
      481 ttaggctgca ccttcctttc ccccactcca ccggagagac cgacaccatg aaaatccaca
      541 ccctggtcgt gaccctggcc gccgcgttcg cgctgccggc gctggcccag cagcagatca
      601 ccgtcgtcaa cttcggcggc gcgaatgcga acgcgcagaa gaaggcctac tacgagccgt
      661 tcgagaagac cggcaccaag gtcgtcgcgg tcgaatacaa cggcgagcag gccaagatca
      721 aggtcatggt cgagaccaag aaggtcacct gggacgtggt cgaggtcgag tcgcccgacg
      781 tggcgcgcgg ctgcgacgaa ggcctgttcg agaagatcga ctacagcaag gtcgcgccga
      841 aggccgactt tctgccggcc gcggtgaccg aatgcggcgt cggcgtcttc gtgtggtcga
      901 cggtgatggc ctggaacggc gacaagctca aggacggccc caagacctgg gccgacttct
      961 gggacaccaa gaaattcccc ggcaagcgcg gcatgcgcaa gggcgcgcgc tacaacctcg
     1021 aattcgcgct gatggccgat ggcgtcaagc cggccgacgt ctacaaggtg ctcgccacca
     1081 aggaaggcgc ggagcgcgcg ttcaagaagc tcggcgagtt gaagtcgagc atccagtggt
     1141 gggaggccgg tgcgcagccg ccgcagttcc tcgtcgccgg cgacgtggtc atgaccaccg
     1201 tctacaacgg ccgcatcgat gccgccaacc gcgaaggcaa gaacctgaag atcggctgga
     1261 ccggcggcat ctacgacctc gactactggg tcatcccgaa gggcgcgccg aacaaggccg
     1321 cttcgctgaa gttcatcgcc ttcgcgagca cgcccgaggc gcaggccgag tacgcgaaga
     1381 acatcgccta cggcccgacc aacaccaagg cgctcgccaa gctcgacgcc aaggtgctgg
     1441 cgaatctgcc gacctcaccg gccaactcga aagacgcgct gcagttcggc ctgaagttct
     1501 gggccgacca gggcgaggac ctcgagaagc gcttcgcctc ctgggccacg aactgatcgg
     1561 ccgggccggg aacgccgccg catgaacgcc agcatcaccg cccccctgcc cggccccgcc
     1621 gacccgctgg gtttgcgcgg ccagctggcc cgtgccgaac ggcgccgcaa gtggcgcgcg
     1681 ttctcgctga cgctgccgct gctgctgttc ctgctgctga cctttctcgt gccgatcgcc
     1741 gcgctgctca agcgggcgat cgagaacccc gaggtcgcca atgccttgcc gcgcaccgtc
     1801 gtggcgctgc aaggctggaa acccgcggac gtgcccgagc ccgcggcgtt cgcggcgatc
     1861 acccaggacc tcggcgccct caccgacagc tccgatgccg gcgccctcgc gcgccgcctg
     1921 aacagcgaga cgcccggcgc gcgctcgctg atcatggcga ccttcaaggc gctgccgctc
     1981 gaaggcacga gcgccacgtc gcccgcggcc gacatcaaga ccgcgctgct cgcgctcgac
     2041 gcgcgctggg gcgaaaccaa atactggcag gcgatcgcga agaacggctc gcgctggacg
     2101 cccgactacc tgctgacctc ggtcgacctg cggcgcgacg cgcaaggcgc gatcgagcgt
     2161 gtcgaccccg cgcagcgtgt gttcggcggc atcctggtgc gcaccttcga gatcagcgcg
     2221 atcgtcacgc tgtggtgcct gctgctgggc tacccgctcg cctactggct gagcacgctg
     2281 tcggcgcgcc aggccaacgt gctgatgatc ctggtgctgg tgccgttctg gacctcgatt
     2341 ctggtgcgcg tcgcggcctg gatcgtgctg ctgcaatccg agggcctggt caaccacgcg
     2401 ctgatcgacc tgcacctgat cgacaagccg ctcgcgctgc tgttcaaccg caccggcgtg
     2461 atcatctcga tggtgcacat cctgctgccg ttcatgatcc tgccgctgta cagcgtgatg
     2521 acgagcgtgc cgccgaccta cctgcgcgcg gccgtctcgc tcggcagccc gccgctggcc
     2581 gcgttcttcc gcgtctacgt gccgcagacg tatcccggca tcggcgccgg cgcgctgctg
     2641 gtgttcatcc tgagcatcgg ctactacgtc acgccggcgc tgctcggcgg cccgaccgac
     2701 cagatgctga gctactacat cgcgcagtac accaacgtca ccgtcaactg gggcatggcc
     2761 tgcgcgctcg gcgcgctgct gctggccgcc acgctggtgc tctacgcgat ctaccggcgc
     2821 gtgacgaagt ccgaactgag cctggggtga gccgatgagc gcgttgagca aactgctgcc
     2881 ccggttcccc gcatattcga cgccgctcga caagctcggc tggtgggcgc tgcgcttcgt
     2941 ctgcgtcggc gtgctggtgt tcctgctgct gccgatcctg gtgatcatcc cgctgtcgtt
     3001 cagcgacagc tcgttcctcg tctacccgat ccccggctgg tcgctgaagt ggtatgacaa
     3061 cctgttcggc tcggccgagt gggcgcgcgc cgcgaagaac agcttcatca tcgcgccggc
     3121 cgcgacgctg ctcgccaccg tgctgggcac gctcgccgcg gtcggcctgg cgcgcaccga
     3181 cttcccgttc aagggcttgc tgatgagcct gctgatcgcg ccgatggtgg tgccgatcgt
     3241 cgtcgtcggc gtcagcacct acctgttctt cgcaccgctg ggcatggccg acagctacct
     3301 ctcgctgatc atcgtgcacg ccgctttggg cgcgccgttc gtgctgacca ccgtgctcgc
     3361 cacgctgcag ggtttcaacc agaacctggt gcgcgccagc ctgagcctgg gcgagagccc
     3421 gctgcgcacc ttcttccgca tcacgctgcc ggtgatcgcg ccgggcgtgg tctcgggcgc
     3481 gctgttcgcg ttcgcgacct cgttcgacga ggtcgtcgtg gtgctgttcc tggccggccc
     3541 cgagcagctg acgctgccgc gccagatgtt caacggcatc cgcgagaaca tctcgccgac
     3601 catcgccgcg gtcgccacgc tgctgatcat cttcacgacc agcctgctgc tcgcgctcga
     3661 gtggctgcgc ggccggcgcc gctgaacggc gcgcgccggg cggcggccta ctcgtcgttc
     3721 gcggcgctga caggcgccgc gccgccctca ccgaagttcg agaagccggc ccgcccctgc
     3781 gcctgagcgg cggcggccag gcccacggcc cggcgcagca aggcctcggg ttgcgcgccg
     3841 tcctgcggat actggccgat gccgatggcg gtcgccaccg tcaggtcgtc cgcgccgacc
     3901 cgcagcggct gcatcagcgc cg
//
LOCUS       DASRDG010000360         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1087251, whole genome shotgun sequence.
ACCESSION   DASRDG010000360 DASRDG010000000
VERSION     DASRDG010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087251"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..371
                     /gene="pilG"
                     /locus_tag="VFA35_05990"
     CDS             6..371
                     /gene="pilG"
                     /locus_tag="VFA35_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HZT55757.1"
                     /translation="MRVLVIDDSKTIRSTAEMLLKKEGCEVITAVDGFEALAKISDHR
                     PQIIFVDIMMPRLDGYQTCALIKNNATYRATPVIMLSSKDGLFDKARGRVVGSEEYLT
                     KPFTRDELLGAIRRHVVAR"
     gene            426..959
                     /locus_tag="VFA35_05995"
     CDS             426..959
                     /locus_tag="VFA35_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZT55758.1"
                     /translation="MKSPFEILADYERSSLAHAAAATPSQAAAANLWRGVGFRVGGHN
                     LVSSLTEVSEILSFPVVTRVPGACTWLLGVASLRGNLIPLVDLNGFLSGEATSVADSS
                     RVLLVRQSGGNVGLLVDEVVGQRNFSADQRTQAPAQTGAAIEHYIVEHYVHGDTQWER
                     FSIAALVRANAFAQAAA"
     gene            1033..3066
                     /locus_tag="VFA35_06000"
     CDS             1033..3066
                     /locus_tag="VFA35_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZT55759.1"
                     /translation="MSTGAGSGAERASGFNGLLVALLVVSMVVAVVDFIYLSIKTNQD
                     AQASALVTRIQVLSQQLSRQSADAAGGNLDAFASLKHTRDTIADLLMRLKNGDVRTGM
                     GGYADQTSLAAYFTELDQAWTQLSADASKVLDSQEQVLSTAASTDDFNRKTAVLNSRM
                     DEVVKILTERGGSGTQVMTASRQMLYADRMQRRAQAIQLGGEDSPSAAAGLQRDSQFY
                     GVVLAGLINGNADLNIRALDNANAKEILNDINKQWIDLAPQVTKLFEAAPTLQAVKQA
                     ADKASIDSQTLLFKADPLSEHISALGTTRKFPNLWIGLAAAGLAILLVLFLALKLFRD
                     QRTRLGTSAELNRRNEAAIQRLLDEMGSLADGDLTVKATVTEDFTGTIADAVNYAIDA
                     LRSLVTTINETSVQVSAAADETQATALHLADAAEHQARQINTASGAVNEIAASARDVS
                     KNSADIADVAQNSVRIAGKGASIVRQTIAGMDSIREQIQDTSKRIKRLGESSQEIGTI
                     VELINDLAEQTNILALNAAIQAASAGEAGRGFAVVADEVQRLAERASAATRRIETLVS
                     AIQVDTNEAVASMEQTTSEVVGGAKLAEQAGTQLGEIESVSGNLAGLIKNIAITAQRQ
                     SAEVAGVSATMNVISDITTQTASGASHTAESIGNLAQLSADLRRSVADFKLPE"
     gene            3077..>4986
                     /locus_tag="VFA35_06005"
     CDS             3077..>4986
                     /locus_tag="VFA35_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55760.1"
                     /translation="MNLAQETDFTTLGWLKPELDVTLKEARQALERYAANPADMQAMR
                     NCAAGLHTTFGTLNMVELYGAALVVDEMERVARGLADGSIMPREDIYSVLMRGFLQLP
                     DYIERLQSGHKDIPIVLLPLLNDLRAARGEKLLSEAVLFAPNIDAALPEPPTSGLDPQ
                     ALRTAAIGLRNEYQFMLLRWIREPGATAPLARIAQIFDTLYAGSGHDAARRLWWVSRA
                     VAEGLQSGAIEPSVAMKLLFGKLDREIKHLIDSGEVALDTTAAKELAKNLLYYLAHAS
                     ARGERAKAVIEFYNLDHLLPTREELEHAHGSISGHNRALLDSVAGGIREDLIRVKETL
                     NVFLHSRQRDPADLAAQANTLGSLADTFGMLGMGVPRLRVLEQRSAIEQIVHGEIEAD
                     EGNLLDIAGGLLVVEASLEDHISRLGAKPEDAEDAEGQVPRAEVVRLMYVLMKEALVN
                     VTKVKDTILAFAVTPLDHAQLESTPGLLVEVAGALDMLALGDAVKPVNAMVHYIRNEL
                     LERRRVPDPSHIDALADAVASIEYYIEAVREQRGDRERVLGIMCDSLAKLNYWPEIKP
                     SSDDVTPAPAAESVAAAPVAHAPVAQPLIDEELRQVFLDEMDEEIANLRRHFPLWRAQ
                     PEETEDLKIVRRS"
BASE COUNT          919 a         1701 c         1614 g          752 t
ORIGIN      
        1 cgggtttgcg ggtcttggtc atcgacgatt cgaagacgat acgcagcacc gcggaaatgc
       61 tgttgaagaa agaaggctgc gaggtaatca ccgcggtcga cggctttgag gcgctggcga
      121 agatatccga ccatcgcccg cagatcattt tcgtcgacat catgatgccg cggctcgacg
      181 gctaccagac ctgcgcgttg atcaagaaca acgcgaccta tcgcgccacg ccggtgatca
      241 tgctgtcctc gaaggacggg ctgttcgaca aggcgcgcgg cagggtcgtc ggctcggagg
      301 aatacctgac caagccgttc acgcgcgacg aactgctcgg cgcgatacgc cgtcacgtgg
      361 tggcgcgttg atgcgccggc cggcaaaccc aacccgcgaa gcggtgcacg cgcatgcatg
      421 acacggtgaa gtccccgttc gaaattctcg ccgactacga gcgcagcagt ctggcccacg
      481 ccgctgcggc cacgccgagt caggcggctg cggccaatct gtggcgcggc gtcggctttc
      541 gcgtcggcgg ccacaacctc gtctcgagtc tgaccgaggt cagcgaaatt ctttcctttc
      601 cggtggtcac gcgcgtgccc ggggcctgca cctggctgct cggtgttgcc agcttgcgcg
      661 gcaatctgat cccgctggtc gacctcaacg gctttctgtc gggcgaggcg acgagcgtgg
      721 ccgattccag ccgcgtgctg ctcgtgcgcc agagcggcgg caacgtcggt ctgctcgtcg
      781 acgaagtcgt cggccagcgc aacttttcgg cggatcaacg cacgcaggcg ccggcgcaga
      841 ccggcgccgc gatcgagcac tacatcgtgg agcactacgt ccacggcgat acgcagtggg
      901 agcgtttcag catcgccgcg ctggtgcgtg cgaacgcgtt cgcgcaggcg gcggcttagc
      961 ggattggcgt gtgccgggat cgccatggcg cacggtattg gcataccaca aaccagatag
     1021 aggtagcggt agatgagtac aggggcaggt agcggcgccg agcgcgcgag cgggttcaac
     1081 ggcctgctcg tggccttgct cgttgtcagc atggtcgtgg cggtggtcga cttcatttat
     1141 ctgtcgatca agactaacca ggacgcacag gcgagcgccc tggtcacgcg aatccaggtc
     1201 ttgtcgcagc agctctcgcg ccagagcgcc gatgccgcag gcggcaacct cgacgcgttc
     1261 gcgagcctca agcacacgcg cgacacgatc gccgatctgt tgatgcgatt gaagaacggc
     1321 gacgtgcgca ccggcatggg cggttacgcc gaccagacga gcctggcagc ttatttcacc
     1381 gaactcgatc aagcctggac ccagctctcg gccgatgcat cgaaggtgct cgacagccag
     1441 gagcaggtgc tgagcacggc ggcgagcacg gacgacttca atcgcaagac cgcggtgctc
     1501 aactcgcgca tggacgaggt cgtgaagatc ctcaccgagc gcggcggttc gggcacgcag
     1561 gtgatgaccg cctcgcgcca gatgctttac gccgaccgca tgcagcgccg cgcgcaggcc
     1621 atccagctcg gcggcgagga ttcgccctcg gccgccgccg gcctgcagcg cgattcgcag
     1681 ttctacggcg tcgtccttgc cggcctgatc aacggcaacg ccgacctcaa catccgcgcg
     1741 ctggacaacg ccaacgcgaa ggagatcctc aacgacatca acaagcagtg gatcgacctc
     1801 gcgccgcagg tgaccaagct gttcgaagcc gcaccgacgc tgcaggcggt caagcaggcc
     1861 gcggacaagg cctcgatcga ctcgcagacc ctgctgttca aggccgatcc gctgtccgag
     1921 cacatcagcg cactgggcac gacgcggaaa ttcccgaacc tgtggatcgg ccttgccgcg
     1981 gcgggtctgg cgatcctgct cgtgctgttc ctcgcgctca agctgttccg cgaccagcgc
     2041 acgcgcctcg gcacgagcgc cgaactgaac cggcgcaacg aggcggcgat tcagcgcctg
     2101 ctcgacgaaa tgggctcgct cgccgacggc gacctgaccg tgaaggccac ggtgaccgag
     2161 gacttcaccg gcacgattgc cgacgccgtg aattacgcga tcgacgcgct gcgttcgctc
     2221 gtcaccacga tcaacgaaac ctcggtgcag gtctccgcgg cggccgacga aacccaggcc
     2281 accgcgctgc acctcgccga tgcggccgaa caccaggcgc gccagatcaa caccgcctcc
     2341 ggtgcggtga acgagatcgc ggcctcggcg cgcgacgtgt cgaagaactc cgcggacatc
     2401 gccgacgttg cgcagaactc cgtgcgcatc gccggcaagg gcgcctcgat tgtgcgccag
     2461 acgatcgccg gcatggactc gatccgcgag cagatccagg acacctccaa gcgcatcaag
     2521 cgcctcggcg agtcctcgca ggagatcggc acgatcgtcg agctgatcaa cgacctcgcc
     2581 gagcagacca acatcctcgc gctcaacgcg gcgatccagg cggcctcggc cggcgaggcc
     2641 ggacgcggct tcgccgtcgt cgccgacgaa gtgcagcgcc tggccgaacg cgcttcggcc
     2701 gcgacccggc gtatcgaaac cctggtcagc gcgattcagg tcgataccaa cgaggcggtc
     2761 gcctcgatgg agcagaccac gagcgaagtg gtcggtggtg ccaagctcgc cgagcaggcg
     2821 ggcacgcagc tcggcgaaat cgaatcggtg tcgggcaacc ttgccggact gatcaagaac
     2881 atcgcgatca ccgcgcagcg ccagtcggcc gaggtcgccg gcgtgtcggc gacgatgaac
     2941 gtgatctcgg acatcaccac ccagaccgcc tcgggcgcaa gccacacggc cgagtcgatc
     3001 ggcaacctcg cccaactttc ggccgacctg cgtcgctcgg tcgccgactt caagctgccg
     3061 gaatgatggc acgtcgatga atctcgccca ggaaaccgat ttcaccacgc tcggctggct
     3121 caagcccgag ctagacgtga cgctgaaaga agcgcgtcag gcgctcgagc gctacgccgc
     3181 caatccggcc gacatgcagg ccatgcgcaa ctgcgccgcc gggttgcaca cgacgttcgg
     3241 cacgctgaac atggtcgagc tgtacggcgc cgcactcgtc gtcgacgaaa tggagcgcgt
     3301 cgcgcgcggc ctcgccgacg gcagcatcat gccgcgcgag gacatctact cggtgctgat
     3361 gcgcggcttc ctgcagctgc cggactacat cgagcggctg cagagcgggc acaaggacat
     3421 cccgatcgta ttgctgccgc tgctcaacga cctgcgcgcc gcgcgcggcg agaagctact
     3481 cagcgaagcg gtgctgttcg cgccgaacat cgatgccgca ttgccggaac cgccgacatc
     3541 cggactcgat ccgcaggcgc tgcgcacggc cgcgatcggc ctgcgcaacg aataccagtt
     3601 catgctgctg cgctggatcc gcgagcccgg cgcgaccgcg ccgctcgcgc gcatcgcgca
     3661 gatcttcgac acgctctacg ccggcagcgg ccacgatgcc gcgcgccgcc tgtggtgggt
     3721 ctcccgcgcc gtcgccgagg gcctgcagag cggcgcgatc gagccaagcg tggcgatgaa
     3781 gctgctgttc ggcaagctcg accgcgagat caagcatctg atcgattccg gcgaagtcgc
     3841 gctggacacg accgcggcca aggaactggc caagaacctg ctctactacc tggcccacgc
     3901 cagcgcgcgc ggcgagcgcg cgaaggcggt gatcgagttc tacaacctcg accacctgct
     3961 gccgacccgg gaagaactcg aacacgcgca tggctcgatc tcgggccaca accgcgccct
     4021 gctcgacagc gtcgccggcg gcatccgcga ggatctgatc cgggtcaagg aaacgctcaa
     4081 cgtcttcctg cactcgcggc agcgcgatcc cgccgacctc gcggcgcagg ccaatacact
     4141 cggcagcctc gccgatacct tcggcatgct cggcatgggc gtgccgcgtc tgcgcgtgct
     4201 cgaacagcgt tccgcgatcg agcagatcgt gcacggcgag atcgaagccg acgaaggcaa
     4261 cctgctcgac atcgccggcg gcctgctcgt cgtcgaagct tcgctcgaag atcacatctc
     4321 gcgcctcggt gccaagccgg aagacgccga ggacgccgag ggccaggtgc cgcgcgccga
     4381 agtggtgcgc ctgatgtacg tgctgatgaa ggaggcgctg gtcaacgtca ccaaggtcaa
     4441 ggacaccatc cttgctttcg ccgtgacgcc gctggaccat gcccagctcg agtccacgcc
     4501 cggattgctc gtcgaggtgg ccggcgcgct cgacatgctc gcgctcggcg acgcagtcaa
     4561 gccggtcaac gcgatggtgc actacatccg caatgaactg ctcgagcggc ggcgcgtgcc
     4621 ggatccgagc catatcgacg cgctcgccga tgcggtcgct tcgatcgagt actacatcga
     4681 ggccgtgcgc gaacagcgcg gcgaccgcga gcgcgtgctc gggatcatgt gcgacagtct
     4741 ggccaagctc aactattggc cggagatcaa gccttccagc gacgacgtga cgccggcacc
     4801 ggcggcagaa tccgtcgccg cggcgccggt ggcgcatgca cccgtcgcgc agccgctgat
     4861 cgacgaggaa ctgcgccagg tctttctcga cgaaatggac gaggagatcg ccaatctgcg
     4921 tcggcacttc ccgctctggc gcgcgcagcc ggaagagacc gaagacctca agatcgtgcg
     4981 ccgctc
//
LOCUS       DASRDG010000361         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1087964, whole genome shotgun sequence.
ACCESSION   DASRDG010000361 DASRDG010000000
VERSION     DASRDG010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087964"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /gene="glmM"
                     /locus_tag="VFA35_06010"
     CDS             complement(<1..430)
                     /gene="glmM"
                     /locus_tag="VFA35_06010"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852352.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZT55761.1"
                     /translation="MTRTYFGTDGIRGTVGQAPITPDFMLRLGHAVGRVLKASDARPT
                     VLIGKDTRISGYMIESALEAGFASAGVDVVLTGPLPTPGVAYLTRALRLDLGVVISAS
                     HNPFADNGIKFFSARGEKLPDAWEAEVESALLAQQRWVGSA"
     gene            complement(456..1298)
                     /gene="folP"
                     /locus_tag="VFA35_06015"
     CDS             complement(456..1298)
                     /gene="folP"
                     /locus_tag="VFA35_06015"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZT55762.1"
                     /translation="MFWQTTRFRIDLARPQVMGIVNATPDSFSDGGVHASAAAAIAHC
                     ELLLAEGADILDIGGESTRPGAAPVGVDEELARVLPVLDAVLTLGCPVSIDTHKAEVM
                     AAVLARGADIVNDINALRSPGALDAVAAHPNCGVCLMHMRGTPASMQDRPRYVDVVDE
                     VAAFLREREAALLARGVDAERIAFDPGIGFGKSVAHNIELLRRQRELLAALGPGRPLL
                     VGWSRKSTLGAITGRDVGERRFASVAAALAAVQSGARIVRVHDVAATVDALKVWQAAG
                     LPAG"
     gene            complement(1354..>2396)
                     /locus_tag="VFA35_06020"
     CDS             complement(1354..>2396)
                     /locus_tag="VFA35_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZT55763.1"
                     /translation="VEMDGFEGNSGVIVIAATNRPDVLDPALMRPGRFDRQVVVPLPD
                     IRGREQILNVHMRKVPVGQDIRADILARGTPGFSGADLANLVNEAALFAARRSGRVVE
                     MNDFEKAKDKIMMGPERKSMIMAEEERKNTAYHESGHALVARLMPKADPVHKVTVIPR
                     GRALGVTMQLPEGDRYSTDKIRMLSTIAILFGGRIAEEVFMGEMTTGASNDFERATHI
                     ARDMVTRYGMTDALGTMVYAENEGEVFLGRSVTKTTTMSEQTMQAVDREIRRIIDEQY
                     AVARKLIEDNKDKIHAMANALLEYETIDSEQIDDIMDGRPPRPPKDWTPSMSKTVPPT
                     PPAAPGTAAATA"
BASE COUNT          336 a          840 c          833 g          387 t
ORIGIN      
        1 cggccgagcc gacccatcgt tgctgcgcga gcagggccga ttcgacctcg gcctcccacg
       61 cgtcggggag cttctcgccg cgcgccgaga agaacttgat cccgttgtcg gcgaacggat
      121 tgtgcgaagc gctgatgacc acgccgaggt cgaggcgaag cgcccgcgtc agataggcga
      181 ccccgggcgt cggcagcggc ccggtgagga cgacatcgac accggccgag gcgaagccgg
      241 cttcgagcgc cgactcgatc atgtagcccg agatgcgcgt gtccttgccg atcagcacgg
      301 tcgggcgcgc gtcgctggcc ttcagcacgc ggccgacggc gtggcccagg cgcagcatga
      361 agtccggcgt gatcggggcc tggcccacgg tgccgcgaat gccgtcggtg ccgaaatagg
      421 ttcttgtcat ggtcgtctcg cctgtgaggc cgggattatc cggccggcag cccggctgcc
      481 tgccatacct tcagcgcatc gacggtggcc gccacatcgt gcacgcgcac gatgcgcgcg
      541 cccgactgca cggccgccag cgccgcggcc acgctggcga accggcgctc gcccacgtcg
      601 cgaccggtga tcgcgcccag cgtcgacttg cgcgaccagc ccaccagcag tggccggccc
      661 ggcccgagcg ccgcgagcag ctcgcgctgc cgccgcagca gctcgatgtt gtgcgccacg
      721 ctcttgccga agccgatgcc cgggtcgaag gcgatgcgct cggcatcgac gccgcgcgcc
      781 agcagggccg cctcgcgctc gcgcagaaag gccgcgactt catcgaccac gtcgacgtac
      841 cgcgggcggt cctgcatcga cgcgggcgtg ccgcgcatgt gcatcaggca cacgccgcag
      901 ttcgggtgag ccgccaccgc gtcgagcgcg ccgggcgagc gcagcgcatt gatgtcgttg
      961 acgatgtcgg cgccgcgcgc gagcacggcg gccatgacct cggccttgtg ggtgtcgatc
     1021 gagaccgggc agcccagtgt cagcaccgcg tcgagcaccg gcagcacgcg cgccagttct
     1081 tcgtcgacgc cgaccggcgc agcgccgggg cgggtcgatt cgcccccgat gtcgaggatg
     1141 tcggcgccct cggccagcag cagctcgcaa tgcgcgatcg ccgcggccgc gctcgcatgc
     1201 acgccgccgt ccgagaacga gtcgggcgtg gcgttgacga tgcccatgac ctgcggccgg
     1261 gccaggtcga tgcggaaacg ggtggtctgc cagaacatga agctcatgag aagggggccc
     1321 cgcagggccc ccgctgtcgt cgttcgcgaa gcgttacgcg gtggctgccg ccgtgcccgg
     1381 cgcagccggc ggcgtgggcg gcacggtctt gctcatcgac ggcgtccagt ccttcggcgg
     1441 gcgcggcggc cggccgtcca tgatgtcgtc gatctgctcg ctgtcgatgg tctcgtactc
     1501 gagcagcgcg ttcgccatcg cgtggatctt gtccttgttg tcctcgatca gcttgcgggc
     1561 cacggcgtac tgctcgtcga tgatgcggcg gatctcgcgg tcgaccgcct gcatggtctg
     1621 ctcgctcatc gtcgtggtct tggtgaccga gcggccgagg aagacctcgc cctcgttctc
     1681 ggcataaacc atcgtgccga gcgcgtcggt catgccgtag cgggtgacca tgtcgcgcgc
     1741 gatgtgggtc gcgcgctcga agtcgttgct cgcgccggtc gtcatctcgc ccatgaacac
     1801 ctcttcggcg atgcgcccgc cgaacaggat cgcgatcgtg ctgagcatgc ggatcttgtc
     1861 ggtgctgtag cggtcgccct cgggcaactg catcgtcacg ccgagcgcgc ggccgcgcgg
     1921 gatcacggtg accttgtgga ccgggtcggc cttcggcatc aggcgcgcga ccagcgcgtg
     1981 gccggactcg tggtaggcgg tgttcttgcg ctcctcctcg gccatgatca tggacttgcg
     2041 ctccggcccc atcatgatct tgtccttggc cttctcgaag tcgttcatct cgacgacccg
     2101 gccgctgcgg cgcgccgcga acagcgccgc ctcgttgacc agattggcca gatcggcacc
     2161 cgagaagccc ggcgtgccgc gcgcgaggat gtcggcgcgg atgtcctggc cgaccggcac
     2221 cttgcgcatg tgcacgttga ggatctgctc gcgaccgcga atatcaggta gcggcaccac
     2281 cacttgccgg tcgaaccggc cggggcgcat gagcgccggg tcgagcacat ctggacggtt
     2341 ggtagccgca atgacgatca cgccggagtt gccctcgaat ccatccatct ccacca
//
LOCUS       DASRDG010000362         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1661240, whole genome shotgun sequence.
ACCESSION   DASRDG010000362 DASRDG010000000
VERSION     DASRDG010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661240"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VFA35_06025"
     CDS             <1..1112
                     /locus_tag="VFA35_06025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZT55764.1"
                     /translation="PPPAELRRQVLDALIDERVQVTNARETGPKLDEAEIDRAVANVA
                     VQNQMTMAQLRARLQQQGLSYGSFRNNLRDQMLVERVREREVNQRIRISDVDVDALIE
                     QRRAANGKSPQLNIAQVLVTVPEGASAEVVEQRRARAEAALTRVKGGEDFGKVAVEVS
                     EDGNKAQGGVIGLRPADRLPDVFVETVKTLRPGEVAPTLLRSGAGFHVLKLVERQAAG
                     AFTVTQTHARHILLRVSAQLSAEAASRRLAEFKRQIQAGTKTFEQLARENSEDGSAAA
                     GGDLGWTGPGTFVPEFEDAMNKLAVGGISDPLVSRFGVHLIQVLERREAVIDLKQQRE
                     QARNVLREQRFEEAYLDWVRDLRGRAYIEMREAPL"
     gene            1210..1974
                     /gene="rsmA"
                     /locus_tag="VFA35_06030"
     CDS             1210..1974
                     /gene="rsmA"
                     /locus_tag="VFA35_06030"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZT55765.1"
                     /translation="MKHVARKRFGQHFLTDGAILDAIVAAVAPQPGQALVEVGPGLGA
                     LTDPLVARCETLTVIELDRDLAARLRRRPELEVIESDVLQVDFAALARQRGQALRVVG
                     NLPYNISTPILFHLLGSVEHILDQHFMLQKEVVDRMAAAPGSKDYGRLSVMLQWRYDI
                     ESVLDVPPQSFDPPPRVDSAVVRMQPLPPAAPVDAALLEELVRVAFSQRRKMLRNTLG
                     RWLDERRFAGAFDLQRRAEEVPVADYLGLVAALTLR"
     gene            complement(1961..2089)
                     /locus_tag="VFA35_06035"
     CDS             complement(1961..2089)
                     /locus_tag="VFA35_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55766.1"
                     /translation="MTPAVADKPAKPAAKSAANNSFGMNMPMMSSAFDTSMWLNAA"
     gene            complement(2131..2526)
                     /locus_tag="VFA35_06040"
     CDS             complement(2131..2526)
                     /locus_tag="VFA35_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HZT55767.1"
                     /translation="MLLQTVRPNIVQSIRAFRVEDLMQAAQGAEQHFLYANLTAAQSK
                     QDVLEAIAQAFMFPAHFGKNLDALYDCMTDLVHKAGAQPGFVVVLEQLPDNPRFDREA
                     REQLLDVFRDAADFWAERKIPFRCFYSFQ"
     gene            complement(2587..2922)
                     /locus_tag="VFA35_06045"
     CDS             complement(2587..2922)
                     /locus_tag="VFA35_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HZT55768.1"
                     /translation="MATPPVQAKGLLPASADTVSLAQLPVEAQTTHRLILAGGPFAHD
                     KDGSVFGNRERSLPRKPRGFYHEYTVRTPGARNRGARRIVCGGNPPTNPEACFYTGDH
                     YASFQRIVQ"
BASE COUNT          483 a          999 c         1043 g          445 t
ORIGIN      
        1 tgccgccgcc ggccgagctg cgcaggcagg tgctcgatgc gctgatcgac gaacgcgtgc
       61 aggtcaccaa cgcacgcgag accggcccga agctggacga agccgagatc gaccgcgcgg
      121 tcgcgaacgt cgcggtgcag aaccagatga cgatggcgca gctgcgcgcg cgcctgcagc
      181 agcaaggcct gtcctacggc agcttccgca acaacctgcg cgaccagatg ttggtcgagc
      241 gcgtgcgcga gcgcgaggtc aaccagcgca tccgcatcag cgacgtcgat gtcgacgcgc
      301 tgatcgaaca gcgccgcgcc gccaacggca agagcccgca gctgaacatc gcgcaggtcc
      361 tggtcacggt gccggaaggc gccagtgccg aggtcgtcga gcagcggcgc gcccgcgccg
      421 aggccgccct gacccgcgtc aagggcggcg aagacttcgg caaggtcgcg gtcgaggtct
      481 cggaagacgg caacaaggcg cagggcggcg tgatcggcct gcgcccggcc gaccggctgc
      541 cggacgtgtt cgtcgagacc gtcaagacgc tgcggccggg cgaggtcgcg ccgacgctgc
      601 tgcgcagcgg cgccggcttc cacgtgctga agctggtcga gcgccaggcg gccggcgcgt
      661 tcaccgtgac gcagacgcat gcgcgccaca tcctgctgcg ggtctcggcg cagctctcgg
      721 ccgaggccgc gagccggcgc ctggccgagt tcaagcgcca gatccaggcc ggcaccaaga
      781 ccttcgagca actcgcgcgc gagaactccg aggacggctc ggccgcggcc ggcggcgatc
      841 tgggctggac gggacccggc accttcgtgc ccgagttcga ggacgcgatg aacaagctcg
      901 ccgtgggcgg catctccgac cccctcgtct cgcgcttcgg cgtgcacctg atccaggtgc
      961 tcgaacggcg cgaggccgtg atcgacctca agcagcagcg cgagcaggcg cgcaacgtgc
     1021 tgcgcgagca gcgcttcgaa gaagcctacc tcgactgggt gcgcgacctg cgcggccgtg
     1081 cgtacatcga gatgcgcgaa gcacctctat gacgacaccc cgctcgccga gtacggcgag
     1141 cgaccctccg agagggtcgg gccttgcttg gggcggcccg gcgccgcggc ccgcgcgccg
     1201 cacttgccga tgaaacacgt cgcgcgcaag cgctttggac agcacttcct gaccgacggc
     1261 gcgatcctcg acgcgatcgt cgcagcagtc gccccacagc ccggccaggc gttggtcgag
     1321 gtcggccccg ggctgggcgc actgaccgac ccgctggtgg cgcgctgcga gacgctgacc
     1381 gtgatcgagc tcgatcgcga cctggcggcg cggctgcgcc gccggcccga gctcgaggtg
     1441 atcgagtccg acgtgctgca ggtcgacttc gccgcgctcg cgcggcagcg cggccaggcg
     1501 ctgcgcgtcg tcggcaacct gccgtacaac atctcgacgc cgatcctgtt ccacttgctc
     1561 ggcagcgtcg agcacatcct cgaccagcac ttcatgctgc agaaggaggt cgtcgaccgc
     1621 atggccgcgg cgccggggag caaggactac gggcgcctga gcgtgatgct gcagtggcgc
     1681 tacgacatcg agtcggtgct cgatgtgcca ccccaatcgt tcgacccgcc gccgcgcgtc
     1741 gactcggcgg tggtgcggat gcagccgctg ccgcccgcgg cgccggtcga tgcggcgctg
     1801 ctcgaagagc tggtgcgggt cgcgttctcg cagcggcgca agatgttgcg caatacgctc
     1861 ggccgctggc tcgacgagcg ccggttcgcc ggcgcgttcg acctgcagcg ccgcgccgag
     1921 gaggtgccgg tggccgacta cctcgggctc gtcgcggctc tcacgctgcg ttgagccaca
     1981 tcgaggtgtc gaaggcgctg ctcatcatcg gcatgttcat gccgaaggag ttgttcgcgg
     2041 cgctcttggc ggccggcttg gccggcttgt cggcgacggc gggggtcatt tcggtagccc
     2101 gctcccaaga cgcgaggtct tgtgagcggg ctactgaaaa gaatagaagc acctgaatgg
     2161 aatttttcgt tcggcccaga agtcggcggc gtcgcggaac acgtcgagca gttgctcgcg
     2221 ggcctcgcgg tcgaagcgcg ggttgtcggg gagttgttcg agcacgacca cgaaaccggg
     2281 ctgcgcgccc gccttgtgga ccaggtcggt catgcagtcg tagagcgcgt ccaggttctt
     2341 gccgaaatgc gcggggaaca tgaacgcctg cgcgattgcc tcgagaacgt cctgcttgga
     2401 ctgggccgcg gtcaggttcg catagaggaa atgctgctcg gcgccttgcg ccgcctgcat
     2461 cagatcttcg acgcgaaacg cgcggatcga ttgaacaatg ttcggacgga ctgtctgcaa
     2521 cagcatggtc acgttcctcc tcgggtcaag aaaaaaactt caaagaacaa aacttcgcgt
     2581 cgccgctcac tgcacgatcc gctgaaagct cgcgtagtgg tcgccggtgt agaaacaggc
     2641 ttccggattc gtgggagggt tgccgccgca aacgatccgc cgcgcaccgc gattgcgcgc
     2701 gccgggcgtg cgaaccgtgt actcgtgata gaagccgcgc ggcttgcgcg gcaacgaccg
     2761 ctcccggttg ccgaacacgc tgccgtcctt gtcgtgggca aacggcccgc cggccaggat
     2821 caggcgatgc gtggtttgcg cctcgaccgg caactgcgcc agcgaaaccg tatcggcgct
     2881 tgccggaagc aggcccttgg cctgaaccgg cggggtcgcc agaaccgcgg ctgccagcgt
     2941 gagaaggagc cccgtctttt gcagagcttg
//
LOCUS       DASRDG010000363         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_763595, whole genome shotgun sequence.
ACCESSION   DASRDG010000363 DASRDG010000000
VERSION     DASRDG010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763595"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..817
                     /locus_tag="VFA35_06050"
     CDS             122..817
                     /locus_tag="VFA35_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HZT55769.1"
                     /translation="MRRRKFTSGAVSAAGLWFLSTGRVAHAAGLTEGDAALGVRAALE
                     RGAASAVALLGKSGGFLDNPKVRIPLPGFLNDAAKVLKYTGQQGRIDELVTAMNRAAE
                     AAVPQAKTLLVNAVKSMSVDDGRRILTGGDNSVTNFFAGKTRAPLSVTFLPIVTKATE
                     KVALADKYNAVAGKAAGLGLVGKDDANIQQYVTGKALDGLYLMIGEEERKIRKDPVGT
                     GSAILQKVFGSLK"
     gene            complement(826..1983)
                     /locus_tag="VFA35_06055"
     CDS             complement(826..1983)
                     /locus_tag="VFA35_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55770.1"
                     /translation="MDLSDVGRTPAAQSVGATARPDDAGDEHERWHDLVAQIGAEIAA
                     PLTAALERINTLTSTGKIDRDGLRALRDEVEAARQAGMIGQQLTRFASGRLRQSHERL
                     QLANVLGGVIAHRTRETTARGVVLKPSLQPADVIVDASLLFGLLNSTVDWALANAQSQ
                     IDFAIDFKNWPAHARLSCRFAHRPIDELVDGADAIAPRGLDSLTWRLMEQTAWTMGLI
                     VERTDAGAMTTLTFEFPRTASPEVEGVSVLELNDGFAPSANSQPLAGSHVLVIASRRE
                     MRVQIRDALRGMSLIIDFVNSVDEAVAFCREGLPHAVIIESIRAGERFAQFRAEVVKE
                     VPDFVFIEIIEEGSTFEMSGLGGGTSIARVGREVLESSLPSALMFELSRGL"
BASE COUNT          332 a          670 c          705 g          311 t
ORIGIN      
        1 cgacattcgg caccttctcg ccgacgctca acgcctggaa cgtcaggtaa ccgccgacgc
       61 cgagaacgac ggcgacaatg gcagaagcaa gggtcttggc aggtttcacg ggagctcttt
      121 catgcggcgt cgaaagttta cgtcaggcgc cgtctctgcg gcggggctgt ggtttctgtc
      181 gacggggcgc gtggcccacg ccgccggcct cactgaaggc gacgccgcgc tcggcgtgcg
      241 cgccgcgctg gagcgcggcg cggcctcggc ggtcgcgctg ctcggcaaga gcggcggctt
      301 tctcgacaac ccgaaggtgc gcatcccgtt gccgggcttc ttgaacgatg ccgcaaaggt
      361 gctgaagtac accggccagc aggggcgcat cgacgagctc gtgaccgcga tgaaccgcgc
      421 cgccgaggcg gcggtgccgc aggcgaagac gctgctcgtc aacgcggtga agtcgatgag
      481 cgtcgacgac gggcggcgga tactgaccgg cggcgacaac tcggtgacga acttcttcgc
      541 cggcaagacg cgcgcgccgc tgtcggtgac cttcctgccg atcgtcacga aggcgaccga
      601 gaaggtcgcg ctggccgaca agtacaacgc cgttgccggc aaggcggccg ggctcgggct
      661 ggttggcaag gacgacgcca acatccagca gtacgtgacc ggcaaggcgc tcgacggcct
      721 gtacctgatg atcggcgaag aggaacgcaa gatccgcaag gatcccgtag gcaccggcag
      781 cgcgatcctg cagaaggtgt tcggctcgct aaagtgagct gagcgctaca agcccctgga
      841 caactcgaac atcaacgccg agggcagcga actctccagc acctcgcgcc cgacgcgcgc
      901 gatcgaggtg ccgccgccga ggcccgacat ctcgaaggtc gagccttctt cgatgatctc
      961 gatgaagacg aagtcgggca cttccttcac gacctctgcg cggaactgcg cgaagcgctc
     1021 gccggcccgg atcgattcga tgatcaccgc gtgcggcagc ccttcgcgac agaacgcgac
     1081 cgcctcgtcg accgaattga cgaagtcgat gatcaggctc atgccgcgca gcgcgtcgcg
     1141 gatctgcacc cgcatctcgc ggcgcgaggc gatcaccagc acgtgactgc cggcgagcgg
     1201 ctgcgaattc gccgagggcg cgaagccgtc gttcagctcc agcacgctaa cgccttcgac
     1261 ctcggggctc gcggtgcgcg ggaattcgaa ggtcagcgtc gtcatcgcgc ccgcgtcggt
     1321 gcgttcgacg atcaggccca tcgtccacgc ggtctgctcc atcaggcgcc aggtgagcga
     1381 gtccagcccg cgcggcgcga tggcgtccgc gccatcgacc agttcgtcga tcgggcgatg
     1441 cgcgaagcgg cagctcaggc gcgcgtgcgc cggccagttc ttgaagtcga tcgcgaagtc
     1501 gatctgagat tgggcgttcg ccagcgccca gtcgacggtc gaattgagca ggccgaacag
     1561 cagcgaggca tcgacgatca cgtcggccgg ctgcagcgaa ggcttcagta caacgccacg
     1621 cgccgtggtt tcgcgcgtgc ggtgggcgat cacgccgccc agcacgttgg cgagctgcag
     1681 gcgttcgtgc gactggcgca ggcggccgga ggcgaagcgg gtcagctgct ggccgatcat
     1741 gccggcctga cgcgcggcct cgacctcgtc gcgcagcgcg cgcaggccgt cgcgatcgat
     1801 cttgccggtg ctggtgaggg tgttgatgcg ctccagcgcg gcggtcagag gcgcggcgat
     1861 ctcggcgccg atctgcgcga ccaggtcgtg ccagcgctcg tgttcgtcgc ccgcgtcgtc
     1921 gggccgcgcg gtggcgccga cgctttgcgc ggccggggtg cggccgacgt cagacaggtc
     1981 catggctcac ctccttcact tcgttctttg cgcgttcg
//
LOCUS       DASRDG010000364         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_286780, whole genome shotgun sequence.
ACCESSION   DASRDG010000364 DASRDG010000000
VERSION     DASRDG010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286780"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1754)
                     /gene="urtB"
                     /locus_tag="VFA35_06060"
     CDS             complement(168..1754)
                     /gene="urtB"
                     /locus_tag="VFA35_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HZT55771.1"
                     /translation="MLRRLLLWSTLVLSAGLLAGPAWALTPQQARALVAGETEDRIAA
                     LNKILEGADEKTVALIQAISNDAVKFTDSAVYVMKDDKGHDPVSGAEVPVPEAAEDVV
                     NNNQMRSELDAALASIKLFSAETSVRADAVKTLAGEPDEAKLPIIEKAYAAEQVPAIK
                     AALAAVRAAMLLGSADPAKRLEAAKSLGATGTPATKTLLLDRQKVETDPAVKGAITAS
                     LDQIEGRLAWGERLGVIFTGISLGSILLLVALGLAITYGLMGVINMAHGELMMIGAYA
                     TYVVQNLFKAYLPGLFDYYVLLAIPASFLAAAAVGAVMERSVIRFLYGRPLETLLATW
                     GISLVLQQAVRSLFGAQNVPVENPAWLSGGIDVMSNLTLPFNRIAIIAFALLVLLGVS
                     ALIARTRLGLFVRGVTQNRKMAACMGVNTARIDTYAFALGSGIAGLAGCALSQVGNVG
                     PDLGQGYIVDAFMVVVLGGVGQLAGTVYAALGLGVLNKLLEGWQGAVLAKIMVLVFIV
                     VFIQKRPQGIFAMKGRSAEA"
     gene            complement(1765..>2556)
                     /locus_tag="VFA35_06065"
     CDS             complement(1765..>2556)
                     /locus_tag="VFA35_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HZT55772.1"
                     /translation="GGGAKRWVLLGTDYVYPRTTNKILRAFLKSKGVADADIDEKYTP
                     FGHSDYQTIVADIKKFSAGGKTAVVSTINGDSNVPFYKELGNAGLKAKDVPVVAFSVG
                     EEELRGVDTKPLVGHLAAWNYFMSIKAPANTAFIKQWSEYAKAKGIAGHKDKPLTNDP
                     MEATYIGIHMWAQAVKKAGSTDTDKVIAAMGGQTFPAPSGITSKMDEKNHHLWKSVFI
                     GEIQADGQFKVVWKTPGPVKANPWSQYIPENKGKKDEPETMAAKK"
BASE COUNT          390 a          872 c          850 g          444 t
ORIGIN      
        1 cgcgtactcg gagagttgga acgcgctgcc ggccggcacc acaaggttca ggaccggtac
       61 cgccaccgcg acgacgacga tcgccgcgag cacgccgctc caggccttgc cggtcagcag
      121 cccacgcgac ggcgccgcga cgggaatggg agacgacaga accgcactca tgcttcggca
      181 ctccgcccct tcatcgcgaa gatgccttgc ggccgcttct ggatgaacac gacgatgaac
      241 accagcacca tgatcttcgc gagcaccgcg ccctgccagc cttcgagcag cttgttcagc
      301 acgcccaggc cgagcgcggc gtagaccgtg ccggcgagct ggccgacgcc gccgagcacg
      361 acgaccatga acgcatcgac gatgtagccc tgccccagat cgggcccgac attgccgacc
      421 tgcgacagcg cgcagccggc cagcccggcg atgcccgaac cgagcgcgaa cgcgtaggtg
      481 tcgatgcgcg cggtgttcac gcccatgcag gcggccatct tgcggttttg cgtgacgccg
      541 cgcacgaaca ggccgagccg ggtgcgcgcg atcagcgccg agacgccgag cagcaccagc
      601 agcgcgaacg cgatgatcgc gatgcgattg aacggcagcg tcaggttgct catcacgtcg
      661 atgccgccgg aaagccacgc cgggttctcg accggcacgt tctgcgcgcc gaacagcgag
      721 cgcacggcct gctgcagcac caggctgatg ccccaggtcg ccagcagggt ctcgagcggc
      781 cggccgtaca ggaagcggat gacgctgcgc tccatcaccg cgccgaccgc cgccgcggcg
      841 aggaacgagg ccgggatcgc gagcagcacg tagtagtcga acagccccgg caggtaggcc
      901 ttgaacaggt tctggaccac gtaggtcgcg tacgcgccga tcatcatcag ctcgccgtgc
      961 gccatgttga tcacgcccat caggccgtag gtgatcgcca ggccgagcgc gaccagcagc
     1021 aggatcgagc cgaggctgat gccggtgaag atcacgccga ggcgctcgcc ccacgcgagg
     1081 cgcccttcga tctggtcgag cgaggcggtg atcgcgcctt tcacggcggg gtcggtctcg
     1141 accttctggc ggtcgagcag caaggtcttg gtggccggcg tgccggtggc gccgagcgat
     1201 ttggcggctt cgaggcgctt ggccgggtcg gcgctgccca gcagcatcgc ggcgcgcacg
     1261 gccgcgagcg cggccttgat cgccggcacc tgctcggccg cgtaggcctt ctcgatgatg
     1321 ggcagcttgg cctcgtcggg ttcgccggcg agcgtcttga ccgcgtcggc gcgcacgctg
     1381 gtctcggccg agaacagctt gatcgatgcc agcgccgcat cgagctcgct gcgcatctgg
     1441 ttgttgttga cgacgtcttc ggccgcctcg ggcaccggca cctcggcgcc gctgaccggg
     1501 tcatggccct tgtcgtcctt catcacgtag acggcgctgt cggtgaactt gaccgcgtcg
     1561 ttcgagatcg cctggatcag cgcgacggtc ttctcgtcgg cgccttccag gatcttgttg
     1621 agcgcggcga tccggtcttc ggtctcgccg gcgacgagcg cgcgcgcctg ctgcggcgtc
     1681 agcgcccagg ccgggccggc cagcaggccg gcactcagca ccagggtcga ccacagcagc
     1741 aggcggcgca gcatcgggcg gctcttactt cttcgccgcc atcgtctcgg gctcgtcctt
     1801 cttgcccttg ttctcgggga tgtactggct ccacgggttg gccttgaccg ggcccggggt
     1861 cttccacacc accttgaact ggccgtcggc ctggatctcg ccgatgaaca ccgacttcca
     1921 caggtggtgg ttcttctcgt ccatcttcga ggtgatgccg ctcggcgccg ggaaggtctg
     1981 gccgcccatc gccgcgatca ccttgtcggt gtcggtgctg ccggccttct tgaccgcctg
     2041 cgcccacatg tggatgccga tgtaggtggc ttccatcggg tcgttggtga gcggcttgtc
     2101 cttgtggccc gcgatgccct tggccttggc gtattcgctc cactgcttga tgaacgcggt
     2161 gttcgccggg gccttgatcg acatgaagta gttccatgcc gcgaggtggc cgaccagcgg
     2221 cttggtgtcg acgccgcgca gctcttcttc accgaccgag aaggcgacga ccggcacgtc
     2281 cttggccttc agcccggcat tgccgagctc cttgtagaac ggcacgttcg agtcgccgtt
     2341 gatggtcgag accaccgcgg tcttgccgcc ggccgagaac ttcttgatgt cggcgacgat
     2401 cgtctggtaa tcggaatgcc cgaagggcgt gtacttctcg tcgatatccg catcagcgac
     2461 acctttcgac ttgaggaacg cgcggaggat cttgttcgtc gtacgaggat agacatagtc
     2521 ggttcccaac agtacccagc gcttggcgcc gccgcc
//
LOCUS       DASRDG010000365         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1316567, whole genome shotgun sequence.
ACCESSION   DASRDG010000365 DASRDG010000000
VERSION     DASRDG010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316567"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFA35_06070"
     CDS             complement(<1..927)
                     /locus_tag="VFA35_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT55773.1"
                     /translation="MTLITKRSLLTRAGWTVLAAAAALAGCGKKDDAPAPSAAAPASA
                     PAAAASAPAAPLKVAFAYLGPAGDHGWTYAHDQAVKAVQKEFGDKIVTSFVENVPESA
                     DAERVFRDMATQGNTLIFGTSFGYMEPMLKVAADFPAAKFEHATGFKQTDNLRSYDSR
                     SYQTAYLAGIIAGKMSKTNTIGVVGSIPVPEVIRNIDAYTLGALSVNPKIKTKVVWVN
                     SWFDPPKETEAAQSLINGGADVLLQNTDSTAVLQTAEKNGKYAFGWDSDMSAFASKAH
                     LGSAVVNWGPIYRKTAKEVIDGTWKTDRQVWGV"
     gene            complement(973..1893)
                     /locus_tag="VFA35_06075"
     CDS             complement(973..1893)
                     /locus_tag="VFA35_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55774.1"
                     /translation="MDGLALLIAASLNAGTALAIAGIGLLINERAGILNLGAEGMMLV
                     AAIAGFATAVHTGNPWLAFGAGMLAGAMLAACFGVLVIWLNTNQVATGLALSIFGGGF
                     SAFVGIPFVSEKLGEHAQFQIPLLGDIPFIGPALFRQHPMVYIAIALTAATAWFLYRT
                     RAGLVLRSIGESPESAHALGYPVRRIRMLAVLAGGAMCGLAGAYISVIYTPLWVEGMI
                     AGKGWIALALTTFATWRPARILLGAYLFGGVTMLQFYLQGRGVHVPSQFLSMLPYLAT
                     VVVLVLISRNAIWIRANMPASLGKAFFPGS"
     gene            complement(1895..>2551)
                     /locus_tag="VFA35_06080"
     CDS             complement(1895..>2551)
                     /locus_tag="VFA35_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55775.1"
                     /translation="VYVADLLLGWLVNGPWQDPNGHNFPQTITFLAQTKLPRLVPGLR
                     AHIGLLVALVAVACFWVFLFRTYRGFALQVGGLAPAAARYAGFSSRSALWVGLLISGG
                     MAGLAGALEAAGPLGQLTPYVTSGYGFAAIIVAYVGRLHPVGIVFSGFLMSLFYIGGE
                     LAQSRLGLPKALTGVFQGLLLFTLLACDTFIFYRIRGRAAPAAAPATPTAIAAAVKEA
                     "
BASE COUNT          422 a          866 c          900 g          363 t
ORIGIN      
        1 cacgccccag acctggcggt cggtcttcca ggtgccgtcg atcacttcct tcgcggtctt
       61 ccggtagatc gggccccagt tgacgaccgc cgacccgagg tgcgccttcg acgcgaacgc
      121 gctcatgtcg ctgtcccagc cgaacgcgta cttgccgttc ttctcggcgg tctgcagcac
      181 cgcggtcgag tcggtgttct gcagcagcac gtcggcgccg ccgttgatca gcgactgcgc
      241 ggcttcggtc tctttcggcg ggtcgaacca gctgttgacc cagaccacct tggttttgat
      301 cttcgggttc acgctcagcg cgccgagggt gtaggcgtcg atgttgcgga tcacctcggg
      361 caccgggatc gagccgacca caccgatggt gttggtcttg ctcatcttcc cggcgatgat
      421 cccggccagg taggcggtct ggtagctgcg gctgtcgtag ctgcgcagat tgtcggtctg
      481 cttgaagccg gtcgcgtgct cgaacttcgc tgcggggaag tcggcggcca ccttcagcat
      541 cggctccatg tagccgaagc tggtgccgaa gatcagcgtg ttgccctgcg tcgccatgtc
      601 gcggaagacg cgttcggcgt cggccgattc gggcacgttc tcgacgaagc tcgtcacgat
      661 cttgtcgccg aactccttct gcacggcctt gaccgcctgg tcgtgcgcgt aggtccaacc
      721 gtggtcaccg gccgggccga ggtacgcgaa cgcgaccttc agcggcgctg ccggggccga
      781 ggcggcggcc gccggcgcgg acgccggtgc agcggccgac ggcgcgggcg cgtcgtcctt
      841 cttgccgcag ccggccaggg ccgcggcggc cgcgagcacg gtccagccgg cccgcgtgag
      901 cagcgaacgc ttcgtgatga gggtcatggg ctctctctcc tgagggggtt gcagtggtgg
      961 aacaagcgag actcaagaac cggggaagaa cgccttgccg agcgacgccg gcatgttggc
     1021 gcggatccag atggcgttgc gcgagattag caccagcacg acgacggtcg cgaggtacgg
     1081 gagcatgctg aggaactgac tcggcacatg cacgccgcgg ccctgcaggt agaactgcag
     1141 catcgtcacg ccgccgaaca ggtacgcacc gagcaagatg cgggccggcc gccaggtcgc
     1201 gaaggtcgtc agcgcgagcg cgatccagcc cttgccggcg atcatccctt cgacccacag
     1261 cggcgtgtag atcaccgaga tgtaggcacc cgccagcccg cacatcgcgc cgccggcaag
     1321 caccgccagc atgcggatgc ggcgcaccgg gtagcccagc gcgtgcgccg attcgggtga
     1381 ctcgccgatc gagcgcagca ccagcccggc acgcgtgcgg tacaagaacc acgcggtcgc
     1441 ggccgtcagc gcgatcgcga tgtagaccat cgggtgctgg cggaacaggg cgggcccgat
     1501 gaacgggatg tcgccgagca gcgggatctg gaactgcgca tgctcgccca gtttctcgga
     1561 cacgaagggg atgccgacga aggccgagaa cccgccgccg aagatgctga gcgccaggcc
     1621 ggtcgcgacc tggttcgtgt tgagccagat caccagcacg ccgaagcagg ccgccagcat
     1681 cgcacccgcg agcatgccgg cgccgaacgc gagccacggg ttgccggtgt gcaccgcggt
     1741 cgcgaaaccg gcgatcgcgg cgaccagcat catcccctcg gcgccgaggt tcaggatgcc
     1801 ggcgcgctcg ttgatcagca agccgatgcc ggcgatcgcg agcgccgtgc ccgcgttcag
     1861 cgacgcggcg atgaggagtg ccaggccgtc catgtcaagc ctccttgact gcagccgcga
     1921 tcgcggtggg cgtcgcgggc gccgccgccg gcgcggcgcg cccgcggatg cggtagaaga
     1981 tgaacgtgtc gcaggccagc agcgtgaaca gcagcaggcc ctggaacacg ccggtcagcg
     2041 ccttcggcag gcccaggcgc gactgcgcga gctcgccgcc gatgtagaac aggctcatca
     2101 ggaaacccga gaacacgatg cccaccgggt gcagccgccc gacgtacgcg acgatgatcg
     2161 cggcgaagcc gtagcccgag gtcacgtaag gcgtgagctg gccgagcggg ccggccgcct
     2221 cgagcgcgcc ggccaggccc gccatgccgc ccgagatcag cagtccgacc cacagcgcgc
     2281 tgcgcgacga gaagccggcg tagcgcgccg ccgccggcgc gaggccgccg acctgcagcg
     2341 cgaagccgcg gtaggtgcgg aacaggaaga cccagaagca cgccaccgcg accagcgcca
     2401 ccagcaagcc gatgtgcgcg cgcagccccg gcaccagccg cggcaacttg gtctgcgcga
     2461 ggaaggtgat ggtctgcggg aagttgtgac cgttcgggtc ttgccacggg ccgttgacga
     2521 gccagccgag cagcaggtcg gcgacgtaga c
//
LOCUS       DASRDG010000366         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_286970, whole genome shotgun sequence.
ACCESSION   DASRDG010000366 DASRDG010000000
VERSION     DASRDG010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286970"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VFA35_06085"
     CDS             <1..1099
                     /locus_tag="VFA35_06085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZT55776.1"
                     /translation="HSKGVVHRDVKPANIFMIGRTQPKVLDFGIARIAHQHEGQQAGD
                     IAAGSPYYMAPEQARHEPVDRRADVFSLGVVLYELLTDLKPFRGNTLAEITAAVLKDD
                     PPLANAIDPAVPEALAQIAAKALAKNPEHRYRSARALARELRHWLDENTVLSEGDVQL
                     GPPRRSGPLWIALGTAAVLALGVIAWAVLGSHRAPALPAASAAVAAPAPVAVASAVVP
                     AAPSASAPAAIVTPGSTMTAAAAAPAASIPAIAPAPASLSAATETPAEPDVAPKEAPR
                     ERRARERAARAAAATTAAAAVPTGALRIAISPWGEVEVDGRRVGTSPPMTELSLPAGR
                     HHVVVRNTDLPPYSAIVNVSAEQPVSLKHKF"
     gene            1102..1491
                     /locus_tag="VFA35_06090"
     CDS             1102..1491
                     /locus_tag="VFA35_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HZT55777.1"
                     /translation="MKILIATTLACAALLAGCALPPCAPAPGLIDLAERPAEKALLAG
                     MRAYDDARYAQAEKLLIQALQVGLVSPRDRAAAHKHLAFIYCTSGRMPACEAEFRAAR
                     LDDAAFTLSRSEAGHPQWGPVWRKVGP"
     gene            1536..1964
                     /gene="ybgC"
                     /locus_tag="VFA35_06095"
     CDS             1536..1964
                     /gene="ybgC"
                     /locus_tag="VFA35_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HZT55778.1"
                     /translation="MHENVSPPFRFQLRVYWEDTDAGGVVFYANYLKFFERARTEWLR
                     SLGHQQEQMRAETGVVFIVTDTTVRYVRPARLDDLIHVTVDVQHAGRAQMTIAQQAWR
                     QPAQGEQTLLAEGTIRIGCVDAETFRPQRIPNAIVESLSP"
     gene            1961..>2315
                     /locus_tag="VFA35_06100"
     CDS             1961..>2315
                     /locus_tag="VFA35_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HZT55779.1"
                     /translation="MNQDFSIFTLVTQASFVVQLVMAGLMIASLASWTVIFGKLFGLK
                     RIRSANEEFEREFWAGKNINDLYADAVKRTDGSPMERIFASGMREFLKLREKRVADAG
                     ALLDGARRAMRASFQR"
BASE COUNT          349 a          865 c          795 g          306 t
ORIGIN      
        1 gcacagcaag ggcgtcgtgc accgcgacgt gaagccggcc aacatcttca tgatcggccg
       61 cacccagccg aaggtgctcg atttcggcat cgcccgcatc gcccaccagc acgagggaca
      121 gcaggccggc gacatcgccg ccggctcgcc gtactacatg gcgcccgagc aggcgcggca
      181 cgagccggtc gaccggcgcg ccgacgtgtt ctcgctcggc gtcgtgctgt acgagttgct
      241 gaccgacctg aagccgttcc gcggcaacac gctggccgag atcaccgccg cggtgctgaa
      301 ggacgacccg ccgctcgcga acgcgatcga cccggcggtg cccgaggcgc tcgcgcagat
      361 cgccgcgaag gcgctggcga aaaaccccga gcaccgctac cgctcggcgc gcgcgctcgc
      421 gcgcgagctg cgccactggc tcgacgagaa cacggtcttg tccgagggcg atgtgcagct
      481 cggcccgccg cgccgcagtg ggccgctgtg gatcgcgctc ggcaccgccg ccgtgctcgc
      541 gctcggcgtg atcgcgtggg ccgtgctcgg ctcgcaccgg gcgcccgcac tgccggcggc
      601 cagcgccgcg gtcgccgcgc cggcacccgt ggcggtcgcg tccgcggtgg tgccggccgc
      661 gccctcggcc tcggccccgg cggcgatcgt cacccccggc agcacgatga cagccgccgc
      721 ggcggcgccg gcggcatcga tcccggcgat cgcgcccgca ccggcatccc tttcagctgc
      781 caccgagacg ccggccgagc ccgatgtcgc gccgaaagag gcgccgagag agcggcgcgc
      841 ccgcgaacgc gcagcgcgcg ccgcggccgc gacgaccgcc gcagccgcgg tgccgaccgg
      901 cgcgctgcgc atcgcgatca gcccgtgggg cgaggtcgag gtcgacggcc ggcgcgtcgg
      961 cacctcgccg ccgatgaccg agctgagcct gcccgccggc cggcaccatg tcgtcgtgcg
     1021 caacaccgac ctgccgccgt acagcgcgat cgtcaacgtg agcgccgagc agccggtcag
     1081 cctcaagcac aagttctgag cgtgaagatc ctcatcgcca cgaccctcgc ctgcgcggcc
     1141 ctgctcgccg gctgcgcgct gccgccgtgc gcgccggccc cgggcttgat cgatctggcg
     1201 gagcgcccgg ccgagaaagc gctgctcgcg ggcatgcggg cctacgacga cgcgcggtac
     1261 gcgcaggcgg agaagctgct gatccaggcg ctgcaggtcg gcctggtgtc gccacgcgac
     1321 cgcgccgcgg cgcacaagca cctcgcgttc atctactgca ccagcggccg catgccggct
     1381 tgcgaggccg agtttcgcgc cgcgcggctc gacgacgcgg cgttcacgct gagccgctct
     1441 gaggccggcc acccgcaatg ggggccggtc tggcgcaagg tgggcccata gcggaccggg
     1501 tcaggccatg ccgggcccgg cgataatcgg gcccgatgca cgagaacgtt tcgccaccct
     1561 tccgatttca gttgcgcgtc tactgggaag acaccgacgc cggcggcgtg gtcttctacg
     1621 ccaactacct gaagttcttc gagcgcgcgc gcaccgagtg gctgcgctcg ctcggccacc
     1681 agcaggagca gatgcgcgcc gagaccggcg tggtcttcat cgtcaccgac acgaccgtgc
     1741 gctatgtgcg gccggcgcgc ctcgatgact tgatccacgt cacggtcgac gtgcagcacg
     1801 ccggccgcgc gcagatgacg atcgcgcagc aggcctggcg ccagccggcg cagggcgagc
     1861 aaacgctgct cgccgaaggc acgatccgca tcggctgtgt cgatgcggaa actttcagac
     1921 cccaacgcat tccgaacgcg atcgtcgaat ccctgtcccc atgaatcaag acttctcgat
     1981 cttcaccctc gtcacgcaag cgagcttcgt cgtccagctc gtgatggcgg ggctgatgat
     2041 cgcctcgctc gcgagctgga ccgtgatctt cggcaagctg ttcgggctga agcgcatccg
     2101 cagcgcgaac gaggaattcg agcgcgaatt ctgggccggc aagaacatca acgatctcta
     2161 cgccgacgcc gtcaagcgca ccgacggctc gccgatggaa cgcatcttcg cctcgggcat
     2221 gcgcgaattc ctgaagctgc gcgagaagcg cgtcgccgac gccggcgcgc tgctcgacgg
     2281 cgcgcgccgc gcgatgcggg cgagctttca gcgcg
//
LOCUS       DASRDG010000367         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_169454, whole genome shotgun sequence.
ACCESSION   DASRDG010000367 DASRDG010000000
VERSION     DASRDG010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169454"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VFA35_06105"
     CDS             <1..1415
                     /locus_tag="VFA35_06105"
                     /inference="COORDINATES: protein motif:HMM:NF006938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HZT55780.1"
                     /translation="AQGTKHPIRVGVIGFVPPQIMQWDKANLEGQVIVRDMAATARRY
                     VPEMRAQGAQLVIAIPHSGLERTWTGELAENEVGQLSLVPGIDAILFGHSHAEFPSKD
                     FADRPNVDIARGTINSVPAVMPGRWGDHLGVIDLDLDNGSGAWKVTTSRSSIRPIYDR
                     AAKRSLAEPDPMVETAIGAEHAQTLAYIRGRVATTPLPIYSYFAQVADDPSVQIVANA
                     QMAYVKRALQGTAYETLPVLSAAAPFKTGGRQGWSYYTDIPAGTLAFRNIADLYMYPN
                     TLKAVLLSGAQVREWLEMSAGQFRRIDPQGAPVQPLLDESFRSYNFDTLDGVSYEFDL
                     TQPARYDGNGKLVAPESHRVQNLRHGGAPVAPDAKFIVATNNYRAFGGGNFPGLDGTN
                     VVLDSPDESRQIVVDWLAQLGGPVNPSADANWRILPVAGVALSFRSGSAATRYLPRHP
                     NIRLVKDNGDGSASFELVDK"
     gene            complement(1434..1904)
                     /locus_tag="VFA35_06110"
     CDS             complement(1434..1904)
                     /locus_tag="VFA35_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZT55781.1"
                     /translation="MQTIHPCLWFDGNAEEAMKFYLGVFPDAKVSDVLLWGDAGPGPK
                     GSVLTCTFELAGQTFMALNGGPQYHFTPAISLVVNCATQAEVDRYWDALLAGGGTPVQ
                     CGWLTDRFGVSWQIVPEAFPRLLQDKDPARADRAMRAMMQMVKLDIAALEKAAG"
     gene            complement(2042..2560)
                     /locus_tag="VFA35_06115"
     CDS             complement(2042..2560)
                     /locus_tag="VFA35_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZT55782.1"
                     /translation="MEAPTDAHGADLDRYDLSILYELQRDARLSNTELAARVGLSAAP
                     TWRRVKWLEEQGFITGYRAEIDRRKIGLGVLAFVRIDADRNTAASTRELEEKIRRIPE
                     VIACHYISGSGTFELQVMATDLDAFSKFSRDTLLHLPHVKDLHTSFSLGEVKAGGALP
                     LTHLRSRQERGR"
BASE COUNT          480 a          980 c          934 g          434 t
ORIGIN      
        1 atgcgcaggg aacgaagcac ccgatccggg tcggcgtgat cggcttcgtc ccgccgcaga
       61 tcatgcagtg ggacaaggcg aacctcgaag gccaagtcat cgtgcgcgac atggccgcga
      121 ccgcgcgccg ctacgtgccc gagatgcgcg cgcagggcgc gcaactcgtc atcgcgatcc
      181 cgcattccgg cctggagcga acctggaccg gcgagctcgc cgagaacgaa gtcggccagc
      241 tctcgctcgt gcccggcatc gacgcgatcc tgttcggcca ctcgcatgcc gagttcccga
      301 gcaaggactt cgccgatcgg ccgaacgtcg acatcgcgcg cggcacgatc aacagcgtac
      361 ccgccgtgat gccgggccgc tggggcgacc acctcggcgt gatcgacctc gacctcgaca
      421 acgggagcgg cgcgtggaag gtgacgacga gccgttcgtc gatccgcccg atctacgacc
      481 gcgcggcgaa gcgctcgctc gccgagcccg acccgatggt ggagacagcg atcggcgccg
      541 aacacgcgca gacgctggcc tacatccgcg gccgcgtcgc gacgacgccc ttgccgatct
      601 acagctactt cgcgcaggtc gccgacgacc cgtcggtgca gatcgtcgcg aacgcgcaga
      661 tggcctacgt gaagcgtgcg ctgcagggca cggcctacga gacactgccc gtgctgtcgg
      721 ccgctgcgcc gttcaagacc ggcgggcgcc agggctggag ctactacacc gacatcccgg
      781 ccggcacgct cgcgtttcgg aacatcgccg atctctacat gtacccgaac acgctgaagg
      841 cggtgctgct gagcggcgcg caggtgcgtg agtggctcga gatgtcggcc gggcagttcc
      901 ggcgcatcga cccgcagggt gcgccggtgc agccgctact cgacgagagc tttcgctcgt
      961 acaacttcga cacgctcgac ggcgtgagct atgaattcga cctgacgcag cccgcgcgct
     1021 acgacggcaa cggcaagctc gtggcgcccg agtcgcaccg ggtgcagaac ctgcgccacg
     1081 gcggcgcgcc ggttgcgccg gacgcgaagt tcatcgtcgc gaccaacaac taccgcgcgt
     1141 tcggcggcgg caactttccc gggctggacg gcacgaacgt cgtgctcgac tcgcccgacg
     1201 agagccgcca gatcgtcgtc gactggctcg cgcaactcgg cggcccggtc aacccgagcg
     1261 ccgacgccaa ctggcgcatc ctgccggtag ccggcgtggc gctgagcttt cgcagcggca
     1321 gcgcagcgac gcgctacctg ccacgccacc cgaacatcag gctggtcaaa gacaacggcg
     1381 acggctcggc gtcgttcgaa ctcgtcgaca agtgagcctg acgtgccgag gcgtcacccc
     1441 gccgccttct cgagcgccgc gatgtcgagc ttgaccatct gcatcatcgc ccgcatcgcg
     1501 cggtcggcgc gcgccgggtc cttgtcctgg agcaggcgcg gaaacgcctc gggcacgatc
     1561 tgccacgaga cgccgaagcg gtcggtcagc cagccgcatt gcaccggcgt gccgccgccg
     1621 gcgagcagcg cgtcccagta gcgatcgacc tcggcctgcg tcgcgcaatt gacgaccagc
     1681 gagatcgccg gcgtgaagtg gtactgcggg ccgccgttca gcgccatgaa ggtctggccg
     1741 gcgagctcga acgtgcaggt gagcaccgag cccttcgggc cggggccggc gtcgccccac
     1801 agcagcacgt cgctcacctt cgcgtccgga aagacgccca ggtagaactt catcgcctct
     1861 tcggcattgc cgtcgaacca caggcagggg tggatggttt gcatcgaagt ttcctttcgg
     1921 tcgaatttcg ttcgaatggc cgggttcgcc gcgcgaaccc gcacgcccat catgatgacg
     1981 acccgggcga cgcgaaatcg acatctcccc gacaccagct tggcgcgacg cggccccgcg
     2041 ctcagcgccc acgctcctgc cggctgcgca gatgcgtcag cggcagcgcg cccccggcct
     2101 tgacctcgcc gagcgagaag ctggtgtgca ggtctttcac gtgcggcagg tgcagcaggg
     2161 tgtcgcgcga gaatttcgag aacgcgtcga ggtcggtcgc catcacctgc agctcgaagg
     2221 tgcccgaccc cgagatgtag tggcaggcga tcacctcggg gatgcggcgg atcttctctt
     2281 cgagttcgcg cgtcgacgcg gcggtgttgc ggtcggcatc gatgcgcacg aacgcgagca
     2341 cgcccaggcc gatcttgcgg cggtcgatct cggcgcggta gccggtgatg aagccttgct
     2401 cctccagcca cttgacgcgg cgccaggtcg gcgcggccga gaggccgacc cgcgccgcga
     2461 gctcggtgtt cgagagccgc gcatcccgtt gcaactcgta caggatcgac aggtcatagc
     2521 gatcgagatc ggcgccgtgc gcgtccgtcg gtgcttccac ggcggcaggg gatttaaccg
     2581 gccttggctt tttgagcgcc atttcctctc cctgcgttga atcggcgcaa gattattgct
     2641 tgaaccgcgc caatccggac aaagatagaa aggacttcac gcgcgcgatt ccctaaactc
     2701 tgcgtcgcgc aacccgtcgt cacgagcggc ggtgagtgtg tgctgaacga accccgtcac
     2761 tgctggagta tcgagaagat gaacgcaccg ctgcccgagt cgatccgcaa ggcgatcgag
     2821 accgtttc
//
LOCUS       DASRDG010000368         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_416465, whole genome shotgun sequence.
ACCESSION   DASRDG010000368 DASRDG010000000
VERSION     DASRDG010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416465"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="VFA35_06120"
     CDS             complement(<1..1787)
                     /locus_tag="VFA35_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZT55783.1"
                     /translation="MKASARATGGAAAEEQRLRTALAQSPRSARLHDKLGVVLARQRR
                     YPEAAQLFERALLLDPTLPHTRRRLGDALAACGRGAEADRHYERFLEQDPDRKAIASG
                     AEHLRAGRRPEAIAAFEAVLRREPDQIDAMRMLALAMMDDPARADDIEALLRRVTELA
                     PDYVAAWNDLGDLYLKSRQWLKGADAFRAAARLEPRNAPSWAGLGDALSQAGCPEPAA
                     EAYRRTIELNPTNAHAHMSLAHVLKDLGQQAQSIASYRGAIKRRPELGEAYWSLANLK
                     TFRFSNEEIAAMEQQVGREGIPPASAVHFCFALGKAHEDRGDYDAAWQWYERGNQQQR
                     PLQSHDPLIMEKRHTTIIDTFSAEFLRAHAGQGYVDARPIFIVGLHRSGSTLIEQILA
                     SHSQVEGTAELPNLGQIAASVGRYRSDRLGFPEAVRDLKPRDWRAYGRQYIDDTRRHR
                     TTGKPSFTDKMPENFPLVGFLHLILPNACIIDARRHPLDSLLGNYKQLYGGGMVYSYD
                     LEELAHYYVEYDRVMRHWQQVLPGKVLTVHYEDTVLDLEGQVRRILEHCGLAFEEACL
                     RYHENPRTVRTASSEQVRRPIYTESLGAWR"
     gene            complement(1784..2602)
                     /gene="hutG"
                     /locus_tag="VFA35_06125"
     CDS             complement(1784..2602)
                     /gene="hutG"
                     /locus_tag="VFA35_06125"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HZT55784.1"
                     /translation="MSQPVQSLATHTLHAGDAPLVISVPHAGIELPPALALRLTPAAL
                     AFPDTDWHVASLYDFAPALGVTMIVARHSRYLIDLNRPPDDAALYGAAAKTGLCPTHS
                     FAGEPLYRDGSTELAPAEIEQRRAQYWQPYHDALATLLDATRRRYGYALLLDAHSIRS
                     VVPRLFAGRLPDVNVGTYDGRSCAAAVTEAVRNCLAAAPGWSQVIDGRFKGGHITRHF
                     GRPGERVHALQIELAQCAYMDEVGAGYDPERAAPLRALLRAIIEALLRMRLRSA"
     gene            complement(2637..>3005)
                     /locus_tag="VFA35_06130"
     CDS             complement(2637..>3005)
                     /locus_tag="VFA35_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HZT55785.1"
                     /translation="TAAALASENKALAHPSSVDSLPTSANQEDHVSMATYAARRLLTM
                     ADNAAGIIAIELLAAAQGVDFHRPLRSSPALEAAHALLRRRVPMLRRDRYFAPDISAA
                     RELITSGALRATLSANLFAR"
BASE COUNT          442 a          986 c         1083 g          494 t
ORIGIN      
        1 cgccaggcgc ccagggattc ggtgtagatc gggcggcgca cctgttcgga actggcggtc
       61 ctgacagtgc gcggattctc gtgatagcgc aggcacgcct cctcgaaggc caggccgcaa
      121 tgctcgagga tgcgccgcac ctggccttcg aggtccagca cggtgtcctc gtaatgcacc
      181 gtcagcacct ttccgggcag cacctgttgc cagtgccgca tcacacgatc gtattccacg
      241 tagtaatgcg ccagctcctc gaggtcgtag ctgtaaacca tgccgccgcc atagagctgc
      301 ttgtagtttc cgagcaggct gtcgagcgga tggcgccggg cgtcgatgat gcaggcattg
      361 ggcaggatca ggtgcaggaa cccgaccagc gggaagttct ccggcatctt gtccgtgaag
      421 ctgggcttgc cggtcgtgcg atggcggcgg gtatcatcga tgtactggcg gccgtaggcg
      481 cgccagtccc tgggcttcag gtcgcgcacg gcttcgggaa aaccgaggcg atcactccgg
      541 taccggccca cggaagcggc gatctgtccc aggttcggca gctcggccgt gccctcgacc
      601 tgcgagtgcg aggccaggat ctgctcgatc agggtcgagc ccgaacggtg cagcccgacg
      661 atgaagattg gccgggcgtc gacatagccc tggcccgcgt gcgcgcgcag gaattcggcg
      721 ctgaacgtat cgatgatcgt ggtatggcgt ttttccatta tcagtggatc gtgcgactgc
      781 agcggccgtt gctgctggtt gccgcgctcg taccactgcc acgccgcgtc gtagtcaccg
      841 cgatcctcgt gagccttgcc gagcgcgaag cagaagtgca ccgccgaggc agggggaatc
      901 ccctcgcgcc cgacctgctg ttccatggcg gcgatctcct cattcgagaa gcggaaagtc
      961 ttgaggttcg cgaggctcca gtacgcttcg ccaagctcag gccgccgctt gatcgcgcca
     1021 cgataggaag cgatggattg cgcctgctgc ccgaggtcct tgagcacgtg cgcgagcgac
     1081 atatgcgcgt gggcattggt cgggttcaac tcgatggtcc gtcgataagc ctctgcggcc
     1141 ggctctgggc agcccgcctg agacaaggcg tcaccgagtc cggcccacga gggtgcattg
     1201 cggggctcta gccgggccgc agcccggaac gcgtcggccc cttttaacca ttggcgggat
     1261 ttgaggtaga ggtccccgag atcgttccag gcggccacgt agtcgggcgc cagctcggtc
     1321 acgcgccgca gcaacgcctc gatgtcgtcc gcgcgcgccg gatcgtccat catcgcgagc
     1381 gcgagcatcc tcatggcatc gatctggtcg ggctcccggc gcagcacggc ttcgaaggcc
     1441 gcgatcgcct cgggccggcg cccggcgcgc aggtgttcgg ccccggatgc aatggccttg
     1501 cggtcgggat cctgctccaa gaagcgctca tagtggcggt cggcttccgc gccacgtccg
     1561 caggccgcga gcgcgtcgcc gaggcgccgg cgcgtgtgcg gcaacgtggg atcgagcagc
     1621 agcgcccgct cgaacaactg tgcggcttcc gggtaacgcc gctgccgcgc gagcacgacg
     1681 cccagtttgt catgcagccg cgccgagcgg ggcgattgcg ccagcgcggt tctcaggcgc
     1741 tgctcctcgg ccgccgctcc tcccgtggcg cgtgccgagg ccttcatgcc gaacgcagcc
     1801 gcatccggag gagcgcctcg atgatggcgc gcagcagcgc gcgcagcggt gcggcacgct
     1861 cgggatcgta gcccgcgccg acctcgtcca tgtaagcgca ttgcgcgagc tcgatctgca
     1921 gcgcgtggac ccgttcgccc gggcgtccga agtggcgcgt gatgtggccg cccttgaagc
     1981 ggccgtcgat gacctgggac cagcctggtg cggcggccag gcagttgcgc acggcctcgg
     2041 tgaccgcggc cgcgcaggaa cggccgtcgt acgtgccgac gttgacgtcc ggcagccggc
     2101 cggcgaacag gcgtggcacg acgctgcgaa tcgagtgcgc gtcgagcaat agcgcgtagc
     2161 cgtagcgccg ccgcgtcgcg tcgagcagtg tagccagcgc gtcgtggtag ggctgccagt
     2221 actgggcgcg gcgctgctcg atctccgccg gcgcgagttc cgtgctgccg tcgcggtaca
     2281 gcggctcgcc ggcaaagctg tgcgtggggc agagcccggt cttcgcggcg gcgccgtaga
     2341 gcgcggcatc gtcgggcggg cgattcaggt cgatcaggta gcgcgagtgg cgcgcgacga
     2401 tcatcgtcac gccaagcgcc ggcgcgaaat cgtagaggct ggccacgtgc cagtcggtgt
     2461 ccgggaatgc gagcgccgca ggcgtgagac gtagtgccag cgcggggggc agttcgatgc
     2521 cggcatgcgg cacgctgatc acgagtggcg catcgccggc gtgcagcgtg tgcgtggcaa
     2581 ggctctgcac cggctggctc atgtcgatga ttgcgtccgc gcccggctcg cctcgctcag
     2641 cgcgcgaaca ggttcgcgct cagcgtggcg cgcagcgcgc cgctcgtgat gagctcgcgc
     2701 gccgcgctga tgtccggcgc gaaatagcga tcgcgcctga gcatcggcac gcgccggcgc
     2761 agcagtgcat gcgccgcctc gagcgcgggc gagctgcgca gtggccgatg gaagtcgacg
     2821 ccctgcgccg cggccagcaa ctcgatggcg atgatgccgg cggcattgtc cgccatcgtc
     2881 agcaggcgtc gcgcggcgta ggtggccatc gagacgtgat cttcctggtt ggcggaggtc
     2941 ggcaggctgt cgacgctgga cggatgcgcc agcgccttgt tctcggaggc cagcgcggcg
     3001 gccgt
//
LOCUS       DASRDG010000369         4306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1662037, whole genome shotgun sequence.
ACCESSION   DASRDG010000369 DASRDG010000000
VERSION     DASRDG010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662037"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="VFA35_06135"
     CDS             <1..1689
                     /locus_tag="VFA35_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZT55786.1"
                     /translation="RFDGERVHLPQASHDASRAYAESGMLAAAQDYEIGGMQLPFVVQ
                     MAANGFFAHAGGNVAGGAMLTSGNANLLMAYGTKLQRQVFAANEFGGRFSGTMCLSEP
                     QAGSSLSDVATRATPDGDDFEHDPLGPRYRLRGNKMWISNGEHELTENIVHLVLAKIP
                     GPDGKLVAGVRGISLFIVPKKLVDEKGELTGERNDVALAGLNHKLGYRGIPNTLLNFG
                     EGRYPVRGEAGGGLDGAGAGAVGYLVGQPGEGLRCMFHMMNEARIAVGLGATMIGYAG
                     YEASLDYAKTRPQGRPIGIGGKDATKPQVPIIEHADIKRMLLAQKSYCEGALALALYC
                     ARLVDEQASGTPEAAAEAGLLLEMLTPIAKSWPSEWCLEANSLAIQVHGGYGYTRDFP
                     VEQYWRDNRLNMIHEGTHGIQALDLLGRKVVMQEGAGLALLVGKVSATIARARAVPAL
                     AAHAEALAAALAHLGAATRAAWSTGSPVEALANATPYLQAFGHTVLAWIWLDVALCAR
                     ARLAQATTADAALLQGKLAACTYFFRYELPRIGAWLKVVETRDPTCRTMDEAWF"
     gene            1689..1892
                     /locus_tag="VFA35_06140"
     CDS             1689..1892
                     /locus_tag="VFA35_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55787.1"
                     /translation="MRTAKRLQTWAWVLIYAGMVLVALGLSVRRSDATLGWGIAGPGV
                     TLIAIGIVLIWVRSRMKDPKETP"
     gene            1889..2686
                     /locus_tag="VFA35_06145"
     CDS             1889..2686
                     /locus_tag="VFA35_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZT55788.1"
                     /translation="MNTPVRKLFDLTGKTALITGGSRGLGLQIAHALGEAGAKVMLSS
                     RKAEDLEVAAAELQDAGIDARWIAADCAKEDQIRRLADETVERIGPIDILVNNAGASW
                     GAPAEEHPVEAWDKVMNLNVRGYFILSQQVARNSMIPGRRGGRIINIASIAGLNGNAP
                     DMKTIAYNTSKTAVIGFTRTLAAEWGRYGINVNAICPGFFMTKMAAGLIETIGEEKMA
                     AAAPLGRLGDDDDLKGIALLYASQAGKHITGQWLAVDGGVSVVLGAA"
     gene            2694..3131
                     /locus_tag="VFA35_06150"
     CDS             2694..3131
                     /locus_tag="VFA35_06150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZT55789.1"
                     /translation="MSISFGVAVPFVEHLGFTLEKFEGGESELRYTAAPEHLNSFAVT
                     HGGACMTLLDVTMAVAARSVQQDMGVVTIEMKTSFMQPARGPLVAKGRLIHRSATLAF
                     TEGTVFDAQGRACSHATGTFKYVKRLPVGATEVHALQPALSTD"
     gene            3160..4176
                     /locus_tag="VFA35_06155"
     CDS             3160..4176
                     /locus_tag="VFA35_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZT55790.1"
                     /translation="MTTNQQIHLVSRPTGEASVDNFKLVEAEVPALKDGQVLVRHHFL
                     SLDPYMRGRMNDAKSYATPQPLNAVMQGGTVGEVVASKNPGYQVGDKVVGFGGWQQFS
                     VVDAGAPGVLRKVDTTHIPLSAYLGAVGMPGVTAWYGLTQICKPTAGKTIAVSAASGA
                     VGSAVGQLAKARGCRAVGIAGGADKCAYVVDELGFDACVDYKAHTDPKSLYEAVKAAT
                     PDGVDGYFENVGGAVLDAVLARMNAFGRIALCGMISGYDGEPIPLKQPQLLLTNRLTL
                     EGFIVAEHMALWPEALKELGTAVATGKLKYRESVAQGLAAAPEAFIGLLKGRNFGKQV
                     VKLT"
BASE COUNT          735 a         1461 c         1516 g          594 t
ORIGIN      
        1 cgcttcgatg gcgagcgtgt ccatttgccg caggccagcc acgacgcgtc gcgggcctac
       61 gccgagtccg gcatgctggc cgcggcgcaa gactacgaga tcggcggcat gcagctgccc
      121 ttcgtcgtgc agatggcggc caatggcttc ttcgcccacg caggcggcaa cgtggccggc
      181 ggcgcgatgc tcacgagcgg caacgcgaac ctgctgatgg cgtacggaac aaagctgcaa
      241 cggcaggtgt tcgcggccaa cgagttcggt gggcgcttct ccggcacgat gtgcctgagc
      301 gagccgcaag ccggctcgag cctgtcggat gtggcgacgc gcgcgacgcc cgacggcgac
      361 gacttcgagc acgatccgct cggcccgcgc taccgcttgc gcggcaacaa aatgtggatc
      421 agcaacggcg aacacgagct gaccgagaac atcgtgcacc tggtgctcgc gaagatcccc
      481 ggcccggacg ggaagctggt cgcaggcgtc cgcggcatct cgctgttcat cgtgccgaag
      541 aagctggtgg acgagaaggg cgagctcacc ggcgaacgca acgacgtggc gctcgcgggc
      601 ctgaaccaca agctcggcta ccgcggcatc ccgaacacgc tgctgaactt cggcgaaggc
      661 cggtacccgg tgcgaggcga agccggcggc ggactcgacg gcgccggtgc cggcgccgtg
      721 ggctacctcg tcggccaacc cggcgaaggc ctgcgctgca tgttccacat gatgaacgag
      781 gcgcgcatcg cggtgggcct gggtgcgacc atgatcggct acgccggcta cgaggcgtcg
      841 ctcgactacg cgaagacgcg gccgcagggc cggccgatcg gcatcggcgg caaggacgcg
      901 acgaagccgc aggtgccgat catcgagcac gccgacatca agcgcatgct gctcgcgcag
      961 aagagctact gcgaaggcgc gctggcgctg gcgctgtatt gcgcgcggct cgtcgacgag
     1021 caggcgagcg gcacacccga ggccgcggcc gaggccggcc tgctgctcga gatgctgacg
     1081 ccgatcgcga agagctggcc gagcgagtgg tgcctcgagg cgaacagcct cgcgatccag
     1141 gtgcacggcg gctacggcta cacgcgcgac ttccccgtcg agcagtactg gcgcgacaac
     1201 cgcctgaaca tgatccacga gggcacgcac gggatccagg cgctcgacct gctcggccgc
     1261 aaggtcgtga tgcaggaggg tgccgggttg gcgctgctcg tgggcaaggt cagcgcgacg
     1321 atcgctcgcg cccgcgcggt gccggcgctg gccgcgcacg ccgaggcgct ggccgcggcg
     1381 ctggcgcatc tgggcgcggc gacgcgggcc gcgtggtcca ccggcagccc tgtcgaggcg
     1441 ctcgcgaacg cgacgccgta cctgcaggcc ttcggccaca ccgtgctcgc atggatctgg
     1501 ctcgatgtcg cgctgtgcgc gcgggcccgg ctcgcgcagg ccaccaccgc cgacgccgcg
     1561 ctgctgcagg gcaagctcgc cgcctgcacc tacttcttcc gctacgaact gccgcgcatc
     1621 ggcgcgtggc tgaaggtcgt cgagacgcgc gacccgacct gccgcacgat ggacgaggcc
     1681 tggttctgat gagaacggcc aagcgcctcc agacctgggc gtgggtcctg atctacgccg
     1741 gcatggtcct cgttgcgctc ggcctgtcgg tgcggcgcag cgacgccacg ctcggctggg
     1801 gcatcgccgg ccccggcgtc acgctgatcg cgatcggcat cgtgctgatc tgggtccgct
     1861 cgcgcatgaa agacccgaag gagacaccat gaacacaccg gtccgaaaac tcttcgatct
     1921 caccggcaag accgcgctga tcaccggcgg ctcgcgcggc ctcggcctgc agatcgcgca
     1981 cgcgctcggc gaagcgggcg cgaaggtgat gctgagttcg cgcaaggccg aagacctcga
     2041 agtggctgcc gccgagctgc aggacgccgg catcgacgcg cgctggatcg cggccgactg
     2101 cgcgaaggaa gaccagatcc ggcgcctcgc cgacgaaacc gtcgaacgca tcgggccgat
     2161 cgacatcctg gtgaacaacg ccggcgcgag ctggggcgca cccgccgagg agcatccggt
     2221 cgaggcgtgg gacaaggtga tgaacctgaa cgtgcgcggc tatttcatcc tgtcgcagca
     2281 ggtcgcgcgc aacagcatga tcccgggcag gcgcggcggc cgcatcatca acatcgcgtc
     2341 gatcgccggg ctcaatggca acgcgccgga catgaagacg atcgcgtaca acacctcgaa
     2401 gaccgccgtg atcggcttca cccgtacgct cgccgccgag tggggccggt acggcatcaa
     2461 cgtcaacgcg atctgcccgg gcttcttcat gacgaagatg gcggccgggc tgatcgagac
     2521 gatcggcgag gagaagatgg ccgcggccgc tccgctcggc cggctgggcg acgacgacga
     2581 cctgaaaggc atcgcgctgc tgtacgcgag ccaggccggc aagcacatca ccgggcaatg
     2641 gctcgcggtc gatggcggcg tgagcgtggt gctcggcgcg gcgtgagggc gcaatgagca
     2701 tctcgttcgg cgttgccgtt ccgttcgtcg aacacctcgg cttcacgctc gagaagttcg
     2761 agggtggcga gtcggaactg cgctacaccg ccgcgcccga gcacctgaac agctttgcgg
     2821 tcacgcacgg cggcgcgtgc atgaccttgc tcgacgtgac gatggcggtc gccgcgcgca
     2881 gcgtgcagca agacatgggc gtggtcacga tcgaaatgaa gaccagcttc atgcagccgg
     2941 cccgcgggcc gctggtcgcg aagggccgct tgatccaccg cagcgccacg ctggccttca
     3001 ccgaaggcac ggtgttcgac gcgcagggcc gcgcatgctc gcacgcgacc ggcaccttca
     3061 agtacgtcaa gcggctgcct gtgggcgcca ccgaggtcca cgcgctgcag ccggcgcttt
     3121 ccaccgattg aatcccgaga gaatttcgag aggacgacca tgaccacgaa ccagcagatc
     3181 cacctcgttt cgcgcccgac cggtgaggcc agcgtcgaca acttcaagct cgtcgaggcc
     3241 gaagtgcccg cgttgaagga cggccaggtg ctggtgcgcc accacttcct gagcctggac
     3301 ccgtacatgc gcggccgcat gaacgacgcg aagagctatg ccacgccgca gccgctgaac
     3361 gcggtgatgc aaggcggcac ggtcggcgaa gtggtggcct cgaagaaccc tggttatcag
     3421 gtcggcgaca aggtcgtcgg cttcggcggc tggcagcagt tcagcgtcgt cgacgcgggc
     3481 gcgccgggcg tgctgcgcaa ggtcgacacc acgcacatcc cgctgtcggc ctacctcggc
     3541 gcggtcggca tgccgggcgt gaccgcgtgg tacgggctga cgcagatctg caagcccacg
     3601 gcgggcaaga cgatcgccgt cagcgcggcc agcggcgcgg tcggcagcgc ggtcggccag
     3661 ctcgcaaagg cgcgcggctg ccgcgcggtg ggcatcgccg gcggcgccga caaatgcgcc
     3721 tacgtggtcg acgagctcgg cttcgacgcc tgcgtcgact acaaggcgca caccgatccg
     3781 aagtcactct acgaggcggt caaggcggcc accccggacg gcgtcgacgg ctacttcgag
     3841 aacgtcggcg gcgcggtgct cgacgcggtg ctggcgcgca tgaacgcgtt cggccgcatc
     3901 gcgctgtgcg gcatgatcag cggctacgac ggcgagccga tcccgctcaa gcagccgcag
     3961 ctgctgctga cgaaccggct cacgctcgag gggttcatcg ttgccgagca catggcgttg
     4021 tggcccgagg cgctgaagga actcggcacc gcggtcgcca ccggcaagct caagtaccgc
     4081 gagtcggtcg cgcaaggcct cgccgcggcg cccgaggcct tcatcggcct gctgaagggc
     4141 aggaacttcg ggaagcaggt cgtcaagctc acctgaggcc cgcatgagca gcatcttgct
     4201 cggcacgcat gaggtgctca accagagcac gccgcttgcc gggcacaacg tctatgggac
     4261 cgatcgggcg ctgcgcgacg cgctgatgtt ccacgcgccg caagcc
//
LOCUS       DASRDG010000370         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_511652, whole genome shotgun sequence.
ACCESSION   DASRDG010000370 DASRDG010000000
VERSION     DASRDG010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511652"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..878
                     /locus_tag="VFA35_06160"
     CDS             126..878
                     /locus_tag="VFA35_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HZT55791.1"
                     /translation="MKFSRNPPKAGEKIRLHVVRCNPADPASVAHLQTYELEQSEGMT
                     LFIALSEIGDRIDPSLQFDFVCRAGICGSCAMVINGRPGLACRTLTKNVGPDITLAPL
                     PVFKLIGDLSVDTGTWMRAMSERLQTWVHLKQQEVDLQRLEERMDPDLAEKIYELDRC
                     IECGCCVSACGTARMREDFVGAVGLNKIARFRLDPRDTRSDDDYYELIGDDNGVFGCM
                     SLLGCHDVCPKQLPLSTQIAFVRRKMVAMAFK"
     gene            925..1953
                     /locus_tag="VFA35_06165"
     CDS             925..1953
                     /locus_tag="VFA35_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZT55792.1"
                     /translation="MNAATSRRRLVALVAALGGALAISVGAQAQAPIVIKFSHVVAND
                     TPKGKASEFFAKRAAELTKGKVKVDVYANSTLYKDKEEMEALQLGSVQMLAPSLSKFA
                     PLGVKEFEIFDFPYIFDSYADLHKITEGPVGKSLMAKLDAKGISGLAFWDNGFKSFSS
                     NRKMQKPEDMKGLKMRIQSSKVLEAQMRALGAIPQVLAFGEVYQALQTGVVDGTENPH
                     SNLYTQKMNEVQKNITITDHGYIGYAVVVNKKFWEGLPADIRGQLTQAMADATKFTNE
                     DALKDNEGALAKVKATGKTEILTLTKEERAVWKRALVKVHKEMESRVGTEIIQAVYKE
                     TGFDPTKV"
     gene            2045..>2126
                     /locus_tag="VFA35_06170"
     CDS             2045..>2126
                     /locus_tag="VFA35_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZT55793.1"
                     /translation="MKVLDHLEEWLISTLMGLATIIIFVAV"
BASE COUNT          428 a          687 c          682 g          329 t
ORIGIN      
        1 aggaacgaga ggatcgacga gcggctggcg ccgcgcccgc gcaacggcgc ggaagaagtc
       61 gctgaaagcg gcttcctgtg ggaaagcgag tagccgtcat gacgaatccc cttcccggaa
      121 caccgatgaa gttctcccgg aatccgccga aggccggcga gaagatccgc ctgcacgtcg
      181 tgcgctgcaa cccggccgac ccggccagcg ttgcgcacct tcagacctac gaactcgagc
      241 agtcggaagg gatgacgctg ttcatcgctc tcagcgagat cggcgacagg atcgacccgt
      301 cgctgcaatt cgacttcgtc tgccgcgccg gcatctgcgg cagctgcgcg atggtgatca
      361 acggccgccc cgggctcgcc tgccgcacgc tcacgaagaa cgtcgggccc gacatcacgc
      421 tggcgccgct gccggtgttc aagctgatcg gcgacctgtc ggtcgacacc ggcacctgga
      481 tgcgtgcgat gagcgagcgg ctccaaacat gggttcacct gaagcagcag gaggtcgacc
      541 tgcagcggct cgaggagcgg atggacccgg acctcgccga gaagatctac gagctcgacc
      601 gctgcatcga gtgcggctgc tgcgtgtcgg cctgcggcac cgcgcgcatg cgcgaagact
      661 ttgtcggcgc cgtcgggctc aacaagatcg cgcgcttccg cctcgacccg cgcgacacgc
      721 gcagcgacga cgactactac gagctgatcg gcgacgacaa cggcgtgttc ggttgcatgt
      781 cgctgctcgg ctgccacgac gtctgcccga agcagctgcc gctgtcgacc cagatcgcat
      841 tcgtgcgccg caagatggtc gcgatggctt tcaagtgaag cggggccgag acgctcatcg
      901 attttcgttc gaaggagaag tttcatgaat gcagcaacat cacgccgccg gttggtggcg
      961 ctcgtcgccg ccctgggcgg tgcactcgcg atctccgtcg gcgcgcaggc gcaggcgccg
     1021 atcgtcatca agttcagcca cgtcgtcgcc aacgacacgc ccaagggcaa ggcctccgag
     1081 ttcttcgcca agcgcgcggc cgagctgacc aaaggcaagg tcaaggtcga cgtctacgcc
     1141 aattcgaccc tgtacaagga caaggaagag atggaggcgc tgcagctcgg ctcggtgcag
     1201 atgctcgcgc cgtcgctctc caagttcgcg ccgctcggcg tcaaggaatt cgagatcttc
     1261 gacttcccct acatcttcga cagctacgcc gacctccaca agatcaccga aggcccggtc
     1321 ggcaagagcc tgatggccaa gctcgacgcc aagggcatca gcggcctggc cttctgggac
     1381 aacggcttca agtcgttctc gtcgaaccgc aagatgcaga agcccgagga catgaagggc
     1441 ctgaagatgc gcatccagag ctcgaaggtg ctcgaggcgc agatgcgcgc gctcggcgcg
     1501 atcccgcagg tgctcgcgtt cggcgaggtg taccaggcgc tgcagacggg cgtcgtcgac
     1561 ggcaccgaga acccgcactc gaacctctat acgcagaaga tgaacgaggt gcagaagaac
     1621 atcacgatca ccgaccacgg ctacatcggc tacgcggtgg tggtgaacaa gaagttctgg
     1681 gaagggctgc cggccgacat ccgcggccag ctgacgcagg cgatggccga cgccaccaag
     1741 ttcaccaacg aagacgccct caaggacaac gaaggcgcgc tcgccaaggt gaaggcgacc
     1801 ggaaagaccg agatcctgac cctgaccaag gaggaacgcg cggtctggaa gcgcgccctc
     1861 gtcaaggtcc acaaggagat ggagtcgcgg gtcggcaccg agatcatcca ggcggtctac
     1921 aaggaaaccg gcttcgaccc caccaaggtc tgagccgcgc actccgggga tgcccgcttg
     1981 accgcgggcg gcgcttctgg acgaatggat gctcgcgccg cggcgcgctt ttttctgttc
     2041 gtcgatgaaa gtgctcgatc acctcgaaga gtggctgatc tcgaccctga tggggctcgc
     2101 gacgatcatc atcttcgtcg cggtca
//
LOCUS       DASRDG010000371         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_511706, whole genome shotgun sequence.
ACCESSION   DASRDG010000371 DASRDG010000000
VERSION     DASRDG010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511706"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VFA35_06175"
     CDS             complement(<1..1170)
                     /locus_tag="VFA35_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZT55794.1"
                     /translation="MKTLTRLRPAWAVPLLALACVCVSAALPGATPERGPVRRALPAA
                     PAPTEARVIVKFKADSALMRTLSAGRPAAPPQHAQALSNRLGLSLVDGRPIGARAQVV
                     KAVGMSSQALAERLAAQSDVEYAVVDARKYALAAPNDPLYAAHPSTSPAVGQWYLRAP
                     TSSTIVNTGSVVSALDAEAAWGVTTGSSSVVVAVLDTGVRPDHPDLAPKLRPGYDFVR
                     ADISSDGDGADADPSDPGDFGCGDATSSWHGTQTAGLIGAATNNAIGMAGTGRDVMLL
                     PVRVLGCGGGYDSDIQAAMLWAGALSASPVANPFPAKVLNLSLGAAGACSASYRDVVG
                     QLNNAGVVVVAAAGNDGLAVGTPANCPGVIAVAGLRHAGTKVGYSDLGPEIALAAP"
     gene            complement(1326..2615)
                     /locus_tag="VFA35_06180"
     CDS             complement(1326..2615)
                     /locus_tag="VFA35_06180"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HZT55795.1"
                     /translation="MKNFRWWPVAGMVAAVVALSGCGGGGSDSSSNASLRVANATLTH
                     ASLDLLVNGTSASTATATDTVGNYATPGSGSVTLQLNDNRGATALSTTVPTLTGGNHY
                     TMVAYESGGAVKTAILNEDNPTPATGTAQLRVYNAALDAGKIDVYITAPTVDLATVST
                     PTVTFATTTANASSALLTYSPGTYRVRITKAGDKSDLRADIPSVVVTSQQIATVILTP
                     AAGGALINGSTLIQQGAYAPARNTNARVRLAAAVSGGATVAASASSGAVIDAGSVAPA
                     FGYGYVLVPATSTLSVTVNGAAVTAPTTALKAGADATLLVYGDPGAATATLFDDDNRL
                     PSDATTTKLRLINGITGSTGTLTLTANTAPVGTGIAPGAASGYVSVIGSTNPMNLKLS
                     SSTNSALLSDSTNVLGLNTVYTVLAGGPISAPQLLIR"
     gene            complement(2788..>2974)
                     /locus_tag="VFA35_06185"
     CDS             complement(2788..>2974)
                     /locus_tag="VFA35_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55796.1"
                     /translation="KGLPRIAVEMGATEGWWKYGCAAVVGIDTYGESAPAPVLFEHFG
                     FTAENVAATVRAVLQRR"
BASE COUNT          397 a         1024 c         1118 g          435 t
ORIGIN      
        1 cggcgccgcc agcgcgatct ctgggccgag gtcggagtag ccgaccttgg tgccggcatg
       61 gcgcaggccc gcgaccgcga tcacgcccgg gcaattggcc ggcgtgccga cggcgagccc
      121 gtcgttgccg gcggccgcga cgacgacgac gccggcgttg ttcaattgcc cgaccacgtc
      181 gcggtagctc gcgctgcagg cgccggcagc gcccaggctc aggttgagca ccttggccgg
      241 gaacgggttg gcgaccggcg acgcgctcaa ggccccggcc catagcatcg ccgcctggat
      301 gtcggagtcg tagccgccgc cgcagccgag cacgcgcacc ggcagcagca tcacgtcgcg
      361 gccggtgccg gccatgccga ttgcgttgtt ggtcgcggcc ccgatcagcc cggcggtctg
      421 ggtaccgtgc cagctgctgg tggcgtcgcc gcagccgaag tcgccagggt cggacgggtc
      481 ggcgtcggcg ccgtcgccgt cgctcgagat gtcggcgcgc acgaagtcgt agcccggccg
      541 cagcttgggc gcgagatcgg ggtggtcggg gcgcacgccg gtgtcgagca ccgcgacgac
      601 gacgctgctg ctgccggtgg tgacgcccca cgcggcctcg gcgtcgagcg ccgacaccac
      661 gctgcccgta ttgacgatcg tgctgctggt cggcgcgcgc aggtaccact ggccgaccgc
      721 cggcgaggtg ctggggtgcg cggcgtagag cgggtcgttg ggcgcggcga gcgcgtattt
      781 gcgcgcgtcg acgaccgcgt attcgacgtc gctctgcgcc gcgagacgct cggccagcgc
      841 ctgcgaactc atcccgacgg ccttcacgac ctgggcccgt gcgccgatcg ggcggccgtc
      901 gacgaggctc agcccgagcc ggttcgacag cgcctgcgcg tgctgcggcg gcgcggcggg
      961 gcggccggcg gagagcgtgc gcatcagcgc cgagtcggcc ttgaacttga cgatcacgcg
     1021 cgcctcggtt ggggccggcg cagccggcaa cgcgcgccgc accgggccgc gctcaggcgt
     1081 agcacccggc agcgcggcgc tgacgcagac gcatgccagc gccagcagcg gaacggccca
     1141 ggcggggcga aggcgggtca aggtcttcat gggcgcctca catcgaaaaa caggcgccgg
     1201 atcggcgcct gtcggcagct gcgcgcgcgg cgcagcaagg ggaaaccatt cccctcagtg
     1261 tagaggcgcc ggcggcggca gacgtgccca agaacacaca cgcccgtgcc gcggttctcg
     1321 aatccctacc ggatcagcag ctgcggcgcg ctgatcgggc cgccggccag caccgtgtag
     1381 accgtgttca ggcccagcac attggtcgag tccgagagca gcgcgctgtt ggtcgacgac
     1441 gacagcttca ggttcatcgg gttcgtgctg ccgatcaccg agacatagcc cgaggccgcg
     1501 cccggggcga tgccggtgcc gaccggcgcg gtgttggccg tcagcgtcag cgtgccggtg
     1561 ctgccggtga tgccgttgat caggcgcagc ttggtcgtgg tcgcgtcgct cggcaggcgg
     1621 ttgtcgtcgt cgaacagcgt cgcggtggcc gcgcccgggt cgccgtagac cagcagcgtc
     1681 gcatcggcgc cggccttcag cgcggtcgtc ggcgcggtca ccgcggcgcc gttgacggtg
     1741 acgctcagcg tgctggtggc cggcacgagc acgtagccgt agccgaacgc cggcgccacg
     1801 ctgccggcgt cgatcacggc gccgctgctg gcggaggccg ccacggtcgc gccccccgag
     1861 accgccgcgg ccaggcgcac gcgggcgttg gtgttgcggg ccggcgcgta ggcgccttgc
     1921 tggatcagcg tcgagccgtt gatcagcgcg ccgccagcgg ccggggtcag gatcacggtg
     1981 gcgatctgct ggctggtgac gacgaccgac gggatgtcgg cgcgcagatc ggacttgtcg
     2041 ccggccttgg tgatgcgcac ccggtaggtg ccggggctgt aggtcagcag cgcgctcgac
     2101 gcattggcgg tcgtggtcgc gaaggtcacg gtcggggtcg agacggtggc gaggtcgacc
     2161 gtcggcgcgg tgatgtagac gtcgatcttg ccggcgtcga gcgcggcgtt gtagacgcgc
     2221 agctgtgcgg tgccggtggc cggcgtcggg ttgtcctcgt tcaggatggc ggtcttgacc
     2281 gcaccgcccg actcgtaggc caccatcgtg tagtggttgc cgccggtgag ggtcggcacg
     2341 gtcgtcgaca gcgcggtcgc gccgcggttg tcgttcagct gcagcgtgac gctgccgctg
     2401 cccggcgtcg cgtaattgcc gaccgtgtcg gtggcggtgg cggtcgaggc gctggtgccg
     2461 ttgaccagca ggtcgagcga cgcgtgcgtc agcgtcgcgt tggcgacccg cagcgatgca
     2521 ttcgacgacg aatcactgcc gccgccaccg caacccgaca acgccaccac cgctgccacc
     2581 atgccggcca ccggccacca cctgaagttc ttcacgtctc gaatccttgc tttcgaatgg
     2641 tcaaatgccc cgctcgcgcg gcgcaaaccc ttcggattgg gcctgcgggg tggggcacgg
     2701 tgattgctgc ggcgcgtccg atcgaaaagt aatgtcaagc tcgccgaccg gcgcgcgccg
     2761 cacgcgcgat gataggccga gcaggtttca acggcgctgc agcacggcgc ggacggtggc
     2821 cgcgacgttc tcggcggtga acccgaagtg ctcgaacagc accggcgccg gggccgattc
     2881 gccgtaggtg tcgatgccga ccaccgccgc acagccatac ttccaccagc cctcggtcgc
     2941 gcccatctcg accgcgatgc gcggcaggcc cttc
//
LOCUS       DASRDG010000372         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_861199, whole genome shotgun sequence.
ACCESSION   DASRDG010000372 DASRDG010000000
VERSION     DASRDG010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861199"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFA35_06190"
     CDS             complement(<1..295)
                     /locus_tag="VFA35_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HZT55797.1"
                     /translation="MPQYNPPLRDMQFVMHELLNVVDELKALPAHADIDADTINAVLE
                     EGGKFAATVVAPLNQSGDEEGCMLDKTTHEVKAPAGFKAAYAQFVEGGWPALSC"
     gene            complement(323..955)
                     /locus_tag="VFA35_06195"
     CDS             complement(323..955)
                     /locus_tag="VFA35_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZT55798.1"
                     /translation="MKGRQTRAAILDAALALASQMGLEGLSIGALAEVTQMSKSGVFA
                     HFGSREELQISVVREYHVQFSAEVFEPAMKEPRGLPRLRALFDRWFKRVSIEIDAGCI
                     YISGAVEFDDRPGPVRDALVTMVQTWQDALERAIRIAIDEGHLRADTDPKQMLFEVHG
                     LILALHHDARFLRHAGADARACAAFENVLSLYIAPAAPVRRVGAGTVKSA"
     gene            1266..2252
                     /locus_tag="VFA35_06200"
     CDS             1266..2252
                     /locus_tag="VFA35_06200"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZT55799.1"
                     /translation="MKIAVVGLGAVGGLLAAQLAAAGHAVSALARGATLAAVRARGLR
                     LVRGGRETVARIDVADDAAALGPQELVIVALKGPALADAAASMQTLIGAETVVMPAMN
                     GVPWWFLQAAPAAVALADRTLASVDPGGRIGALLPLERVVGCVVHLTCASPEPGRVQH
                     GFGDRLIVGEPTGGTSDRVDRVAAALRDAGFSTEASTDIRHDIWYKLWGNMTMNPVSA
                     LTGAESHRIIDDPLVLEFMLAAMEEARRIGARIGCAITQSGQERMGVARQLGAFKTSM
                     LQDSLAGRPLEIDALVGAVHEIGSRLDIAMPNIGALLGLTRLMARERGLYPA"
     gene            2382..>2658
                     /locus_tag="VFA35_06205"
     CDS             2382..>2658
                     /locus_tag="VFA35_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZT55800.1"
                     /translation="MEVLQLDISGRPQAWISAKEAAVIYASDGVAWTLGDAFFVLRGG
                     MQRRTGLQSRIEVHPIIAVRGSVPSRAWRQTPALSNPKLFARDRGICA"
BASE COUNT          388 a          890 c          955 g          425 t
ORIGIN      
        1 cgcagctcag cgccggccag ccgccttcga cgaactgcgc gtaggcggcc ttgaagccgg
       61 ccggcgcctt gacctcgtgc gtggtcttgt cgagcatgca gccttcttcg tcaccgctct
      121 ggttcagcgg cgcgaccacg gtggcggcaa acttgccgcc ttcttcgagc accgcgttga
      181 tcgtgtcggc gtcgatatcg gcatgcgccg gcagcgcctt cagttcatcg acgacattga
      241 gcagttcgtg catcacgaac tgcatgtcgc gcagcggcgg gttgtattgg ggcatggtgg
      301 tgaactcctg agtgaaatcg aatcaagcgg atttgacggt gccggcgccc acgcggcgga
      361 ccggcgcagc gggtgcgatg tagagcgaga gcacgttctc gaacgcggcg caggcgcgcg
      421 catcggcacc ggcgtggcgc aggaagcgcg cgtcgtggtg cagcgcgagg atcaggccgt
      481 ggacctcgaa cagcatctgc ttcgggtcgg tgtcggcgcg cagatggcct tcgtcgatgg
      541 cgatccggat cgcgcgttcg agcgcgtcct gccaggtctg caccatcgtg acgagcgcgt
      601 cgcgcaccgg gccgggccgg tcatcgaact cgaccgcgcc gctgatgtag atgcagccgg
      661 cgtcgatctc tatcgagacg cgcttgaacc agcggtcgaa gagcgcgcgc aggcgcggca
      721 ggccgcgagg ctccttcatc gccggctcga acacctcggc cgagaactgc acgtggtact
      781 cgcgcaccac cgaaatctgc agttcctcgc gcgagccgaa gtgcgcgaac acgcccgact
      841 tgctcatctg cgtgacctcg gcgagcgcgc cgatcgacag cccttcgagc cccatctgcg
      901 aggccagcgc gagcgcggcg tcgaggatcg cggcgcgcgt ctggcggccc ttcatcaggg
      961 ggcgcggcga ggcctcggca gtcgtggcgt cgcggtcggt ctcgttcagc agcggcatgg
     1021 gatcggtcat ggcggtcggc cagatgggcc tggcaggtaa aaacgaacgg tcgtgctatt
     1081 ttgccgacga ttgcatgacc ggcgcgtttc gccgggttct atttccggac tttttctagg
     1141 ctgccgcgcc tagacgcccg gccggagcgc ggcgggccac gggtttaccc gcgcaccgtg
     1201 accggtcgag cccgttgcta ccatcgtccg caagcaaaca cgcggccagc cgcgacgagg
     1261 gccggatgaa gatcgcagtg gtgggtttgg gcgccgtcgg cggcctgctg gcggcgcagc
     1321 tggcggcggc cggccacgcg gtcagcgcgc tggcgcgcgg cgcaacgctc gctgcggtgc
     1381 gcgcgcgcgg gctgcggctg gtgcgcggcg ggcgcgagac ggtcgcccgg atcgacgtgg
     1441 ccgacgatgc cgccgcgctc ggcccgcagg agctcgtgat cgtcgcgctg aaaggcccgg
     1501 cgctggcgga cgcggccgcg tcgatgcaaa ccttgatcgg ggcggagacg gtcgtgatgc
     1561 ccgcgatgaa cggcgtgccc tggtggtttt tgcaagccgc gccggcggcc gtggcgctcg
     1621 ccgatcgcac gctcgccagc gtcgaccccg gcgggcgcat cggcgcactg ctgccgctcg
     1681 aacgcgtggt cggctgcgtc gttcacctga cctgcgccag ccccgaaccg ggtcgggtcc
     1741 agcacggctt cggcgaccgg ctgatcgtcg gcgagcccac cggtggcacg tccgatcgcg
     1801 tcgaccgcgt cgccgcggcg ctgcgcgacg ccggcttcag caccgaggcc agcaccgaca
     1861 tccgccacga catttggtac aagctctggg gcaacatgac gatgaacccg gtttcggcac
     1921 tgaccggtgc cgaaagccac cgcatcatcg acgacccgct ggtactcgag ttcatgctcg
     1981 ccgcgatgga agaggcgcgc cggatcggcg cccggatcgg ttgcgcgatc acgcagtcgg
     2041 ggcaggagcg catgggtgtt gcgcgccagc tcggcgcgtt caagacctcg atgctgcagg
     2101 actcgctcgc cggccggccg ctggagatcg acgcgctggt cggcgcggtg cacgagatcg
     2161 gcagccggct cgacatcgcg atgccgaaca tcggcgcgct gctcggcctg acgcggctga
     2221 tggcgcgcga acgcgggctg tacccggcct gatctatccg gcctgaaatg acgcgcccgt
     2281 gacggcgcgg tgcaatttcg agacaccgcg ccctccgccg cgcgtaagcg catgatcggg
     2341 ggttgaggga acacccgctg atgcattacg ctcgaaacct catggaagtt ctgcaactcg
     2401 acatttccgg tcggccgcag gcctggatct ccgcgaaaga agcggcggtg atctatgcga
     2461 gcgatggcgt cgcctggacg ctgggcgatg cgttcttcgt gctgcgcggc gggatgcagc
     2521 gccgcaccgg gttgcagtct cgcatcgagg tgcacccgat catcgcggtg cggggctcgg
     2581 tgccgagccg agcctggcgc cagacgcctg ccctgtcgaa ccccaagctg ttcgcgcgcg
     2641 accgcggcat ctgtgcct
//
LOCUS       DASRDG010000373         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_982023, whole genome shotgun sequence.
ACCESSION   DASRDG010000373 DASRDG010000000
VERSION     DASRDG010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982023"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFA35_06210"
     CDS             complement(<1..363)
                     /locus_tag="VFA35_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55801.1"
                     /translation="MMHLLLPAAFLVAQGAVFALLPAISAPAAYLFMVAAPLLAGAAA
                     LRRGLVERGDARVGWLAVALAMGVWSAGAFCNYWQEVIAGHLYEMHRSAMLAFNLAVV
                     PVTFLLAGGWGGAGRWPAR"
     gene            complement(360..2045)
                     /locus_tag="VFA35_06215"
     CDS             complement(360..2045)
                     /locus_tag="VFA35_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZT55802.1"
                     /translation="MPPIDRYAELHRNFRWLVPAEFNIAQVCCTRWARDTPHAIAIHA
                     EHEDGRRADHSFAELQRAANRLSNALRRLGVARGDRVAIVMPQRFETAAAHVATYQLG
                     AVALPLSMLFGPEALAYRLNDGAARIAIVDESGIANLRVARAACPRLATVLAVGAAQG
                     QGDLDWADACGRERARFAAVNTKCDEGAVLIYTSGTTGPPKGALIPHRALIGNLSGFV
                     ASQNWFPQDDAVFWSPADWAWTGGLMDALLPTLYFGRAIVGYQGRFAPETAFDLMQRH
                     RVTHTFLFPTALKAMMKAVPAPRERYALRLRAIMSAGEAVGDAVFAWCRDALGVPVNE
                     MFGQTEMNYIVGNSHARWPARPGSMGRPYPGHRVAVIDAAGLECPRGTPGDVALHRRD
                     LHGTPDPVFFLGYWNNAAATKAKFTGDPDRSWCRTGDMAVMDADGYLWYRGRSDDVFK
                     AAGYRIGPSEIENCLVKHAAVANAAVVPKPDAERGALVKAYVVLAPGFEGTPALVEQL
                     QRHVRGQLAPYEYPKEIEFVDALPMTTTGKVQRRVLRLQEEARAAAATAERAR"
     gene            complement(2072..>2607)
                     /locus_tag="VFA35_06220"
     CDS             complement(2072..>2607)
                     /locus_tag="VFA35_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55803.1"
                     /translation="ATLAVLVLGGAIYVSGVVPIAHLGKETALGQHMVDGLRSAGRGS
                     FAWRIAQDQKVLPDAMATPVTGSAQWDWWRSKGVRPWGLLMLLLGQFGLVGLCLCFGA
                     LLMPALREAWRAPRASAWQPRGLPLMLATLVVLTVLDGLLNSFLFFPAVTAAGGLAAG
                     GLRPPGEITRIASRSAA"
BASE COUNT          401 a          949 c          926 g          331 t
ORIGIN      
        1 gcgcgccggc caccgcccgg cgccgcccca gccgccggcg agcaggaagg tgaccggcac
       61 gaccgcgagg ttgaacgcga gcatggcgct gcggtgcatc tcgtacaggt gcccggcgat
      121 cacctcctgc cagtagttgc agaacgcgcc ggccgaccag acccccatcg ccaacgccac
      181 cgcgagccag ccgacacgcg cgtcgccgcg ctcgaccagc ccgcggcgca gcgccgccgc
      241 gcccgccagc agcggcgcgg cgaccatgaa caggtaggcg gccggcgcgc tgatcgcagg
      301 cagcagcgcg aacaccgcgc cctgcgcgac gaggaatgcg gccggcagca ggagatgcat
      361 catcgcgcgc gctcggccgt ggcggcggcc gcacgcgcct cttcctgcag gcgcagcacg
      421 cggcgctgca ccttgccggt ggtcgtcatc ggcagcgcgt cgacgaactc gatctccttc
      481 gggtactcgt agggcgcgag ttggccgcgc acatggcgct gcagttgctc gaccagcgcc
      541 ggcgtgccct cgaagcccgg cgccagcacg acgtaggcct tgaccagcgc gccgcgctcg
      601 gcgtcgggct tgggcacgac ggcagcgttc gcgaccgcgg cgtgcttgac caggcagttc
      661 tcgatctcgc tcgggccgat gcggtagccg gcggccttga acacgtcgtc gctgcggccg
      721 cggtaccaga ggtagccgtc ggcatccatc accgccatgt cgccggtgcg gcaccaactc
      781 ctgtccgggt cgcccgtgaa cttggccttc gtggcggccg cgttgttcca gtagccgaga
      841 aagaacaccg ggtcgggcgt gccgtgcaga tcgcgccggt gcagcgccac gtcgccgggc
      901 gtgccgcgcg ggcactcgag gccggcggcg tcgatcaccg cgacgcggtg gcccgggtag
      961 ggccggccca tgctgccggg ccgcgccggc cagcgcgcgt gcgagttgcc gacgatgtag
     1021 ttcatctcgg tctggccgaa catttcgttc accggcacgc caagcgcgtc gcggcaccac
     1081 gcgaacaccg cgtcgccgac cgcctcgccc gcgctcatga tcgcgcgcag ccgcagcgcg
     1141 tagcgttcgc gcggcgccgg caccgccttc atcatcgcct tcagcgcggt cgggaacagg
     1201 aaggtgtgcg tgacgcgatg ccgctgcatc agatcgaacg cggtctcggg cgcgaagcgg
     1261 ccctggtagc cgacgatggc gcggccgaag tacagcgtcg gcagcaaggc atccatcagc
     1321 ccgcccgtcc acgcccagtc cgcggggctc cagaacacgg cgtcatcctg aggaaaccag
     1381 ttctggctcg cgacgaaacc gctgaggttg ccgatcagcg cgcggtgcgg gatcagcgcg
     1441 cctttcggcg ggccggtcgt gccgctggtg tagatcagca cggcaccttc gtcgcacttg
     1501 gtgttcacgg ccgcgaagcg cgcgcgctcg cgcccgcagg cgtcggccca gtcgagatcg
     1561 ccctgcccct gcgccgcgcc gaccgcgagc acggtcgcga gccgcgggca cgccgcgcgc
     1621 gcgacgcgca ggttcgcgat gccgctctcg tcgacgatcg cgatgcgcgc ggcgccgtcg
     1681 ttgagccggt acgcaagcgc ctcgggcccg aacaacatcg acagcggcaa cgccaccgcg
     1741 ccgagctggt aggtcgcgac gtgcgccgcc gcggtctcga agcgctgggg catcacgatc
     1801 gcgacgcggt cgccgcgcgc aacgccgagg cgccgcagcg cattcgagag gcggttcgcg
     1861 gcgcgctgca gttcggcgaa gctgtgatcc gcgcggcggc cgtcttcgtg ctcggcgtgg
     1921 atcgcgatgg cgtgcggcgt gtcgcgcgcc cagcgggtgc agcagacctg cgcaatgttg
     1981 aactcggcgg gcacaagcca gcggaagttg cgatgcagct cagcgtagcg atcgatcggc
     2041 ggcaattgtt tctcctcgtg tccgatcggc ctcaggccgc gcttctggac gcgatgcggg
     2101 tgatctcgcc aggcggacgg aggccgccgg ccgcaagacc gcccgccgcg gtgacggcag
     2161 ggaagaacag gaacgaattc aacagaccgt cgaggacggt cagcacgacc agggtggcca
     2221 gcatcaaggg caggccgcgc ggctgccagg cactggcccg cggcgcccgc caggcctcgc
     2281 gcagggcggg catcagcaat gcaccgaagc aaaggcacag gccgacgagt ccgaactgac
     2341 ccagcaggag catcaacagg ccccaggggc gaacaccctt cgagcgccac cagtcccatt
     2401 gcgccgaacc ggtcacgggc gttgccatcg cgtcgggaag caccttctgg tcttgcgcga
     2461 tgcgccaggc gaacgagccg cggccggcgg accgcaagcc atcgaccatg tgctgcccga
     2521 gcgcggtctc cttgcccagg tgggcgatcg gcacgacgcc ggacacgtag atcgcgccac
     2581 ccagcacgag gaccgccaac gttgccg
//
LOCUS       DASRDG010000374         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_170573, whole genome shotgun sequence.
ACCESSION   DASRDG010000374 DASRDG010000000
VERSION     DASRDG010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170573"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1274
                     /locus_tag="VFA35_06225"
     CDS             171..1274
                     /locus_tag="VFA35_06225"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HZT55804.1"
                     /translation="MDRDAILLTPGPLTTTLRTKLAMLRDWGSWDADFNALTAELRRQ
                     LLAIIDGADSHVVVPLQGSGTFSVEAAVATLVPRDGHLLVLDNGAYCKRLGKVATLMG
                     RRTTIMAHAEDVAVSAEALDARLREDPSITHVGFIHCETGTGVLNPLQAVSDVCKRHG
                     KGLIIDAMSSFAALPIDARALTFDALVAASGKCLEGVPGMGFVFIRKAVLAARENNSH
                     SLAMDLFDQHAYMEKTGQWRFTPPTHVVAALAEALRQFVEEGGRPARLARYADNCATL
                     IDGMKGLGFEPFLAPALQAPIIVTFHAPAVPGYDFKAFYDEVKRRGFILYPGKLTEVE
                     TFRVGCIGAIGRNEMQQAVHAVADAMAALGWSR"
     gene            1286..2674
                     /locus_tag="VFA35_06230"
     CDS             1286..2674
                     /locus_tag="VFA35_06230"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZT55805.1"
                     /translation="MATKPAKNAKEHPALRAVRESGTGKVKVAVSDIDGILRGKYLHI
                     DKFLGAAQPHPNGGFGFCDVVFGWDANDHCYDNATLTGWQHGFPDALARLDLDTYRTV
                     PWDDGVPFFLGDFVTPAGDPSPICPRQTLKRVLKRAEKLGVMTMAGMEFEWFNFAETP
                     QSWAEKKGVGPEPITPGMFGYSLLRMNHKREYFKALMDEMPLFGVPIEGLHTETGPGV
                     YEVAIAFGEALEQADRAVLFKTGAKEIGARFGVMPSFMAKWNQQLPGCSGHIHQSLSD
                     GKSNLFYDAKSPRKMSKLFESYLAGQCGVLMQMAPMFWPTINSYKRLVDGFWAPVKPT
                     WGMDNRTASFRVIAGSPKATRLETRCPGADVNPYLAMAAVIAAGLHGVEQGLKLTQPP
                     ITGTNSGAEDIPRAPRTLIETTRVFRQSDVARDWFGDDFVDHYAATREWEWRQWLDAI
                     TDWELKRYFEII"
BASE COUNT          482 a          978 c          970 g          416 t
ORIGIN      
        1 cacgccggag caccagtacc agggcgagag caaggcctac gcgccggacg gcgaggtcct
       61 gctgaccctg ccgcgcgacg aggccggcct tgccgtcttc gagctcggcg cgcgcgactt
      121 ccgctggacg ccgctgccgg catgacagca caagaacaca ggagaccccc atggaccgcg
      181 acgccatcct gctcaccccc ggcccgttga cgacgacgct gcgcaccaag ctcgcgatgc
      241 tgcgcgactg gggctcgtgg gacgccgact tcaacgcgct gacggccgag ctgcgccgcc
      301 agctgctcgc gatcatcgac ggcgcggact cgcacgtcgt cgtgcccttg cagggcagcg
      361 gcaccttctc ggtcgaggcc gcggtcgcga cgctggtgcc ccgcgacggc catttgctgg
      421 tgctcgacaa cggcgcctac tgcaagcgcc tgggcaaggt cgcgacgctg atgggccggc
      481 gcaccacgat catggcgcat gccgaagacg tcgccgtgtc ggccgaggcg ctcgatgcgc
      541 ggctgcgcga ggacccgagc atcacccacg tcggcttcat ccactgcgag accggcaccg
      601 gtgtgctgaa cccgctgcag gcggtgagcg atgtgtgcaa gcgccacggc aagggtctga
      661 tcatcgacgc gatgagctcg ttcgcggcgc tgccgatcga cgcgcgcgcg ctcaccttcg
      721 acgcactcgt ggccgccagc ggcaagtgcc tcgaaggcgt gccgggcatg ggcttcgtgt
      781 tcatccgcaa ggccgtgctg gcggcccgcg agaacaactc gcactcgctc gcgatggacc
      841 tgttcgacca gcacgcctac atggagaaga ccggccagtg gcgcttcacg ccgccgaccc
      901 atgtggtcgc ggcgctggcc gaggcgctgc ggcagttcgt cgaggagggc ggccggccgg
      961 cccggctcgc gcgctacgca gacaactgcg cgacgctgat cgacggcatg aaggggctgg
     1021 gcttcgagcc cttcctcgcg cccgcgctgc aggcgccgat catcgtgacc ttccatgcgc
     1081 cggccgtgcc gggctacgac ttcaaggcgt tctacgacga ggtcaaacgc cgcggcttca
     1141 tcctctaccc gggcaagctg accgaggtcg agacctttcg cgtcggctgc atcggcgcga
     1201 tcgggcgcaa cgagatgcag caggcggtgc acgccgtggc cgacgcgatg gccgcgctcg
     1261 gctggtcacg ataaggagag tgccaatggc aaccaagccc gcgaagaacg cgaaggaaca
     1321 tccggcgctg cgcgcggtgc gcgagtccgg caccggcaag gtcaaggtcg cggtcagcga
     1381 catcgacggc atcctgcgcg gcaagtacct gcacatcgac aaattcctcg gcgcggcgca
     1441 gccgcatccg aacggcggct tcggcttctg cgacgtggtg ttcggctggg acgcgaacga
     1501 tcactgctac gacaacgcga cgctgaccgg ctggcagcac ggcttcccgg acgcgctggc
     1561 gcggctcgac ctcgacacct accgcacggt gccgtgggac gacggcgtgc cgttcttcct
     1621 cggcgacttc gtcacgccgg ccggcgaccc gtcgccgatc tgcccgcgcc agacgctcaa
     1681 gcgcgtgctg aagcgcgccg agaagctcgg cgtgatgacc atggccggca tggagttcga
     1741 gtggttcaac ttcgccgaga cgccgcagag ctgggccgag aagaagggcg tcggtcccga
     1801 gccgatcacg ccgggcatgt tcggctactc gctgctgcgc atgaaccaca agcgcgagta
     1861 cttcaaggcg ctgatggacg agatgccgct gttcggcgtg cccatcgaag ggctgcacac
     1921 cgagaccggg cccggcgtgt acgaggttgc gatcgcgttc ggcgaggcgc tggagcaggc
     1981 cgaccgtgcg gtcctgttca agacgggcgc gaaggagatc ggtgcgcgct tcggcgtgat
     2041 gccgagcttc atggcgaagt ggaaccagca gctgccgggc tgctcggggc acatccacca
     2101 gagcctctcc gacggcaaga gcaacctgtt ctacgacgcc aagtcaccgc gcaagatgag
     2161 caagctgttc gagagctacc tcgccggtca gtgcggcgtc ctgatgcaga tggcgccgat
     2221 gttctggccg acgatcaaca gctacaagcg cctggtcgat ggcttctggg cgccggtcaa
     2281 gccgacctgg ggcatggaca accgcaccgc gagcttccgt gtcatcgccg gcagcccgaa
     2341 ggcgacgcgg ctcgagaccc gctgccccgg cgccgacgtg aacccgtatc tcgcgatggc
     2401 cgcggtgatc gcggctggcc tgcacggcgt cgagcagggg ctgaagctga cgcagccgcc
     2461 gatcaccggc accaacagcg gcgccgaaga catcccgcgc gcgccgcgca cgctgatcga
     2521 gacgacgcgg gtcttccgcc agtccgacgt ggcgcgcgac tggttcggcg acgatttcgt
     2581 cgaccactac gctgcgaccc gcgaatggga atggcggcag tggctcgacg cgatcaccga
     2641 ctgggaactg aagcggtatt tcgagatcat ttgaagcggc gcgccgtcca ccgaatcacg
     2701 aaaggcactg catgacgatc gagcgattca gctttccgac gacgattcat ttcggtcccg
     2761 gcgcgcgcaa gctcgtcgcc gaacatctga agagccaggg cgtcgcgcgc ccgctgatcg
     2821 tcaccgatcg cggcatcgct cccctg
//
LOCUS       DASRDG010000375         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1439901, whole genome shotgun sequence.
ACCESSION   DASRDG010000375 DASRDG010000000
VERSION     DASRDG010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439901"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..190
                     /locus_tag="VFA35_06235"
                     /pseudo
     CDS             2..190
                     /locus_tag="VFA35_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
     gene            complement(245..958)
                     /locus_tag="VFA35_06240"
                     /pseudo
     CDS             complement(245..958)
                     /locus_tag="VFA35_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
     gene            complement(1012..>2281)
                     /locus_tag="VFA35_06245"
     CDS             complement(1012..>2281)
                     /locus_tag="VFA35_06245"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT55806.1"
                     /translation="AFACYQESAEFLRQAGLHDEPDRADADVLVEYVNTLVKLAWLYV
                     LRNDPRSKTVLDRAESLREHIAQAAEVIASLEQTWGEYWRRGGDLKRALEHKHHALHL
                     YERLGDRQSILKTYGNLSLIYGDAKDFARAIDYSQRVLDMAQRFTVEPETLAATHLHL
                     GATYFWQGKYGPAIEHYQKALEISQRASLSVLVGRAHYNLAEAYYKRSQALDRAEDEA
                     LGDQHTAAALAAWPDGGDPARAEATRKLKGEILGPREGEFYDRLLPGEFAAHFSEMSE
                     VRRQLAALALPLDAPQQVESHLAIAKAYLQIAVKEREAAVALIDKHGFGERFAPVLEQ
                     LRQTFHRGLSREQRLAALWHNAAADLLRPERGHEVLRHVLQEGSVNKSGYARLCGVGL
                     ATASKQLGQLAERGLLVQTGKGPRTRYQLP"
BASE COUNT          355 a          762 c          755 g          409 t
ORIGIN      
        1 cctgctcgcc tgcggcatcg ttgccgcctc tgtgaccgcc catgccgcat accccgacaa
       61 gccgatcacg ctggtcgtgc cgttctctgc aggtggcccg actgacaaga tcgctcgcga
      121 tctggccgag gccctgcgca agccgctggg gcaggtgatc gtggtggaca acacggccgg
      181 cgccggcggc ggccaggtcg ggcacctgcg tgccggggtt ggcgatggtc tcgacgaaga
      241 ccagtcgcgt cgacgggcgc aacgcggcct ccacgccggc cacctcgccg gcatcgacga
      301 agtctacgtc gatgccgaag ccttgcagcg tttggaacag actgcgcgtg ttgccgaaca
      361 ggtgccgact gacgaccagg tggtcgcctg ccttgagccg cgccagcagg atgccggcga
      421 tggccgccat gccggtggaa aagcccgccg cagcgcgact gccttccaac agcgccagct
      481 tgacttccag cgcgccgccg gtgggattgc cctgacgcgc gtagacgaag cccggattca
      541 cgccttggaa caccgccacc agctcggccg ccgtggcgta tccaaaggtg gtggagatgt
      601 gcaccggctt gtgcagcgcc ccgtgctcaa caccggtccg gcggtcggcg tgcagcaggc
      661 ggctcaagtt ggattcggcc ttgggcgatt gggcatcggc agtcatagcc gcaatcgtca
      721 cgccgcgttg gctgacccca tcggcatcgc agtgctgtgg ttccacaacc cgtggaccgg
      781 ctcgccgcca caggggtgat gtggtctccg atcccaaggg cagggcgatc ctgccgccgg
      841 gcgcaatcct gctccacgct ttgcgccagg tgtaggtcgt ggccacagac aagaagcccg
      901 tcgactggga tctggtcaag ccgctgtacc acgccggcat ctgttcgctg aaggacatcg
      961 gcctggagtt cggagtcctt ggcgccaagc aaacactgac gccaagctca tttaaggcag
     1021 ttgatagcgt gtgcgcgggc ccttgcccgt ctgcaccagc aagccgcgtt cggcaagctg
     1081 gcccagttgc ttcgatgccg tggccagacc gacgccgcac agccgtgcgt agccactctt
     1141 attcaccgag ccttcctgca gcacgtgtcg cagcacctca tggccccgtt ccgggcgcag
     1201 caggtcggcc gctgcgttgt gccacaacgc cgccaggcgc tgctcgcggc tgagcccgcg
     1261 gtggaaggtc tggcgcagtt gctccagcac cggtgcgaag cgctcgccga agccgtgctt
     1321 gtcgatcagc gccacagcag cctcgcgttc cttgaccgcg atctgcaggt acgccttcgc
     1381 gatcgccagg tgcgactcga cttgttgcgg cgcatccagc ggcaatgcaa gtgccgcgag
     1441 ctggcgccgc acttcggaca tctcgctgaa gtgcgcggca aattcgcccg gcaacaagcg
     1501 gtcatagaac tcgccctcgc gcggaccgag gatctcgcct ttcagcttgc gcgtggcctc
     1561 ggcccgcgcc gggtcgccgc catcgggcca ggcggccagc gcggcggcgg tgtgttggtc
     1621 gcctagcgct tcgtcttccg cgcggtccag cgcctgggag cgcttgtaat aggcctcggc
     1681 caggttgtag tgcgcgcgcc cgaccagcac gctcaagctg gcacgctggc tgatctccag
     1741 cgccttttgg tagtgctcga ttgccggacc gtacttgcct tgccagaagt aggtggcgcc
     1801 caggtgcagg tgagtggcag ctagggtttc gggttcgact gtgaagcgct gcgccatgtc
     1861 cagcacacgc tgcgagtagt cgatggcgcg ggcgaagtcc ttggcatcgc catagatcag
     1921 gctcaggttg ccgtaggtct tcagaatcga ttgtcggtcg cccagtcgtt cgtacagatg
     1981 cagcgcgtgg tgcttgtgct ccagcgcgcg tttcaggtcg ccgccgcggc gccagtattc
     2041 gccccaggtc tgctccaggc tggcgatgac ctcggccgcc tgcgcaatgt gctcgcgcaa
     2101 ggattcagcg cggtcgagca cggtcttcga tcgtgggtca ttgcgaagca cgtagagcca
     2161 ggccagcttg accagcgtgt tgacatattc gaccagcaca tcggcgtcgg cccggtccgg
     2221 ctcgtcatgc aagccggcct gtcgcaggaa ctcggcgctt tcctggtagc aagcaaaggc
     2281 g
//
LOCUS       DASRDG010000376         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_982759, whole genome shotgun sequence.
ACCESSION   DASRDG010000376 DASRDG010000000
VERSION     DASRDG010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982759"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..531)
                     /locus_tag="VFA35_06250"
     CDS             complement(301..531)
                     /locus_tag="VFA35_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55807.1"
                     /translation="MPLNRTVKSVTPRRLRPANLRCRGGASSVLRLTQPTAARLPTSF
                     DLSDWMSHAASVLMPVTGPHFDLGAHRGASGV"
     gene            complement(776..>2393)
                     /locus_tag="VFA35_06255"
     CDS             complement(776..>2393)
                     /locus_tag="VFA35_06255"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HZT55808.1"
                     /translation="FDVPENTTVALVGESGSGKSVTAMSILNLLPENAERTGAITFQG
                     RDMLATPLAELQKLRGREIACVFQDPMSSLNPVFTVGQQLCEPLVKHLGMKPRQAMAR
                     AEALLVEVGMPEPKRRLSVYPHQLSGGQQQRVMIAMALACEPKLLIADEPTTALDVTI
                     QRQIMELLAGLKSKHQMSMLFISHDLGVVGEIADQVVVMRHGIVREQGAVAGIFAHPQ
                     DAYTKALLACRPTLEQRAPRLMVIDAHIAAQGNNGVAAHAAAGVAKPKDPNARVIIEA
                     RALTKSFWLHSGVFGRKEFKAVKGATFQLRQGHTLGVVGESGSGKTTMGLALLRLHEP
                     SGGPVGGQVTFDGIDLLRLSKGEMLPMRKRIQVVFQNPYASLNPRFTVGQTLIEPMTI
                     HGIGKDPDEREARARALLVKVGLPEEAMDKYPHEFSGGQRQRIAIARCLTLDPEVLVL
                     DEAVSALDVSVQAQVLNLLKDLQDELGLAYIFISHDLAVVRFMADEVLVMKDGEVVEQ
                     ASVEQILDAPQQEYTRRLLGAIPRGYRTAA"
BASE COUNT          363 a          829 c          821 g          380 t
ORIGIN      
        1 gaccaccacg agaaagaaaa gaagggaagc gggacagtga tttgccggcg ctcgctctcg
       61 actccgcacc agcctgcccg cccccctctg gacgcccgtc cgtccgcgaa aactgagatt
      121 ccgtcgaccc gcgtcagcgg ctcggccgaa ggaacagcga caactgtgcc ggcgcccggc
      181 cggtttgcag cacgcgaaac ggctggcgct gcggttgcag gaccggtgcc ttgcgctgtg
      241 gcagcaccgc ggcggcggca gcgccctgtc gggtcgacgg cgaagaagcg ttcatggtca
      301 tcaaaccccc gaggcgccgc ggtgcgcgcc gagatcgaag tgcgggcccg tcaccggcat
      361 cagcacgctc gcggcatggc tcatccagtc ggacagatcg aagctcgtcg gcaggcgggc
      421 cgcggtcggc tgcgtcaggc gcagcaccga cgacgcgcca ccgcggcagc gcaggttggc
      481 aggccgcagg cggcggggcg tgacggactt cacagtgcgg ttcagcggca tgacaacgac
      541 gtcctcttct gagtgctcgg cccggcgccg ctcgaggcgg catctcgggt gctgtgagtc
      601 acacctgcgc aacgaccgtg ccatggcgac gaacctctcc ttcggggcgt gccggacaaa
      661 atttgacgcc gaatctcaca agtccctgcg aaatgtgacg cactcgtaaa agatgcgtta
      721 cagggtcgac cggattcgtc ggggtctgcg cggccgcgcg gtacccgcgc gagggtcacg
      781 ccgccgtgcg gtagccgcgc ggaatggccc ccagcagcct gcgcgtgtac tcctgctgcg
      841 gcgcgtcgag gatctgttcg acgctggcct gctcgacgac ctcgccatcc ttcatcacca
      901 gcacctcgtc ggccatgaag cgcaccacgg ccaggtcgtg gctgatgaag atgtaggcga
      961 ggccgagctc gtcctgcagg tccttcagca ggttcagcac ctgcgcctgc accgagacgt
     1021 cgagcgcgct gaccgcctcg tcgagcacca gcacctcggg gtcgagcgtc aggcagcgcg
     1081 cgatcgcgat gcgctggcgc tggccgccgg agaactcgtg cgggtacttg tccatcgctt
     1141 cctcgggcag gccgaccttg accagcagcg cgcgggcgcg ggcctcgcgc tcgtcggggt
     1201 ccttgccgat gccgtggatc gtcatcggct cgatcagcgt ctggccgacg gtgaagcgcg
     1261 ggttcagcga agcgtaaggg ttctggaaca cgacctggat gcgcttgcgc atcggcagca
     1321 tctcgccctt gctcaggcgc agcaggtcga tgccgtcgaa cgtgacctgg ccgccgacgg
     1381 gcccgccgct gggctcgtgc aggcgcagca gcgccaggcc catcgtcgtc ttgcccgagc
     1441 ccgattcgcc gacgacgccg agcgtgtggc cctggcgcag ctggaaggtc gcgcccttca
     1501 cggccttgaa ctccttgcgg ccgaacacgc cgctgtgcag ccagaagctc ttcgtcagcg
     1561 cgcgggcctc gatgatcacg cgcgcgttcg ggtccttcgg cttggcgacc ccggctgcag
     1621 cgtgcgccgc gacgccattg ttgccctgcg ccgcgatgtg cgcgtcgatc accatcagcc
     1681 gcggcgcgcg ctgctcgagc gtcggccggc aggcgagcag ggccttggtg taggcgtcct
     1741 gcgggtgcgc gaagatgccg gcgacggcgc cctgctcgcg cacgatgcca tggcgcatca
     1801 cgaccacctg gtcggcgatc tcgccgacca cgccgaggtc gtggctgatg aacagcatgc
     1861 tcatctggtg cttcgatttc agcccggcga gcagctccat gatctggcgc tggatcgtca
     1921 cgtcgagcgc ggtggtcggt tcgtcggcga tcagcagctt cggctcgcag gccagcgcca
     1981 tcgcgatcat cacgcgctgc tgctggccgc ccgagagctg gtgcgggtag accgacaagc
     2041 gtcgcttcgg ctccggcatg cccacctcga ccagcagcgc ctcggcacgc gccatcgcct
     2101 gccgtggctt catccccagg tgcttgacga gcggctcgca gagctgctgg ccgacggtga
     2161 agaccgggtt cagcgaactc atcggatcct ggaacacgca ggcgatctcg cggccgcgca
     2221 gcttctgcag ttcggccagc ggggtggcga gcatgtcgcg gccctggaac gtgatcgccc
     2281 cggtgcgctc ggcgttctcc ggcaacaggt tcaggatcga catggcggtc acgctcttgc
     2341 ccgagccgga ctcgccgacc agcgcgacgg tcgtgttctc cggcacgtcg aaa
//
LOCUS       DASRDG010000377         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_59197, whole genome shotgun sequence.
ACCESSION   DASRDG010000377 DASRDG010000000
VERSION     DASRDG010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59197"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFA35_06260"
     CDS             <1..660
                     /locus_tag="VFA35_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZT55809.1"
                     /translation="LVYFQTLLRGYRLSDLTVVYPVARGTGPLLSSLGALVLLGERLS
                     LVGAAGVIAVTLGVFFIAGGPGLWAKAHDPAQRRRVRAGLGWGAATGALIAGYTLIDG
                     YAVKAMLISPLLVDYFGNLLRLPFLLPAALRDPATLGAVWRAQWKHALVVALLAPLAY
                     VLVLYAVKLAPLSHVAPAREVSMLFAALLGGRLLGEGDRGLRIAGAVCIAAGVAALAL
                     G"
     gene            668..1489
                     /locus_tag="VFA35_06265"
     CDS             668..1489
                     /locus_tag="VFA35_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HZT55810.1"
                     /translation="MNADRRDALAGIDFTSRPTRAKPITVALGHLADGVVRLERIETH
                     ADFDGFAHWLRTPGPWLGVFDLPFGLPRELVVALGWPLQWRPLIAHYAALSRAQIRAT
                     FKAFCDARPAGGKFAHRAADGPAGSSPSMKWVNPPVAFMLHAGVPLLIEAGMHLPGLH
                     AGDARRVALEGYPGLLARELLGARPYKSDTRAKQTPERLIARIDLVDALDQGRTRLGL
                     RLRLTHAQRESLIDDARGDRLDAVLCLMQAAWASTRANHGLPAAIDPLEGWIVSA"
     gene            1558..>2303
                     /locus_tag="VFA35_06270"
     CDS             1558..>2303
                     /locus_tag="VFA35_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZT55811.1"
                     /translation="MYTFNLLLLAGGLLVFVSLLAGVASTRLGLPFLLVFLGAGMLVG
                     VDGPVGVRFDDVKLAAALGNAALAMILLEGGISTRMEVFRAGFRPAFALATIGVVLTA
                     AVVGAAAMAALHVDWRHGLLLGAIVGSTDAAAVFPLLRQTGLRLSERVAATLELESGL
                     NDPMAVFLVLALIATLKTDAGGAELLLLFARQAGFGAAIGLGAGWAVAWLLAKLPLDV
                     EHGGVLSLIVVSAAMAVFATCGLIEGSGFLA"
BASE COUNT          237 a          825 c          862 g          379 t
ORIGIN      
        1 ctcgtgtact tccagacgct gttgcgcggc taccggctgt cggacctgac cgtggtctac
       61 ccggtcgcgc gcggcaccgg gcccttgctg agctcgctgg gcgcgctggt gctgctcggc
      121 gagcggctca gcctcgtcgg cgcggccggc gtgatcgcgg tgacgctcgg cgtgttcttc
      181 atcgccggcg gccccgggct gtgggccaag gcgcacgacc cggcgcagcg ccgccgcgtg
      241 cgcgccgggc tcggctgggg cgcggccacc ggtgcgctga ttgccggcta cacgctgatc
      301 gacggctacg cggtcaaggc gatgctgatc tcgccgctgc tggtcgacta cttcggcaac
      361 ctgctgcgcc tgccgttcct gctgccggcc gcgctgcgcg acccggcgac gctcggcgcg
      421 gtctggcgcg cgcaatggaa acacgcgctc gtcgtcgcgc tgctggcgcc gctcgcctac
      481 gtgctggtgc tctacgccgt gaagctcgcg ccgctcagcc atgtcgcgcc cgcgcgcgag
      541 gtctcgatgc tgttcgccgc gctgctcggt ggtcggctgc tcggcgaggg cgaccgcggc
      601 ctgcgcatcg ccggcgcggt ctgcatcgcc gccggcgtgg cggcgttggc gctgggctga
      661 ggccagcatg aacgcggatc gacgcgacgc gctcgccggc atcgacttca cgagccggcc
      721 gacgcgcgcc aagccgatca cggtggcgct cggccacctg gccgatggcg tggtgcggct
      781 cgagcgcatc gagacccacg ccgacttcga cggcttcgcg cactggctgc gcacgccggg
      841 gccgtggctc ggcgtgttcg acctgccgtt cggactgccg cgcgagctcg tcgtcgcgct
      901 cggctggcca ctgcagtggc ggccgctgat cgcgcactac gcggccttgt cgcgcgcgca
      961 gatccgcgcc acgttcaagg cgttctgcga tgcgcggccg gccggcggca agttcgcgca
     1021 ccgcgccgcc gacgggccgg cgggttcgtc gccgtcgatg aagtgggtca acccgccggt
     1081 cgcgttcatg ctgcatgccg gcgtgccctt gctgatcgag gccggcatgc acctgcccgg
     1141 gctgcacgcc ggcgacgcgc gccgcgtcgc gctcgaaggc taccccggcc tgctggcgcg
     1201 cgaactgctc ggcgcgcgcc cctacaagag cgacacgcgc gcgaagcaga cgcccgagcg
     1261 cctgatcgcg cgcatcgacc tggtcgacgc gctcgaccag ggccgcaccc gcctcggcct
     1321 gcgtctgcgg ctcacgcacg cgcagcgcga gtcgctgatc gacgacgcgc gcggcgaccg
     1381 cctcgacgcc gtgctgtgcc tgatgcaggc cgcgtgggcg agcacgcgag cgaaccacgg
     1441 cctgccggcc gcgatcgacc cgctcgaagg ctggattgtc agcgcctgac ggcgcagacc
     1501 tggcgcagcg tgtcgtccga caggcggcgc gggcgtgctc tgctaaggtc aaccgccatg
     1561 tacacgttca acctgctgct gctcgcgggc gggctgctgg ttttcgtcag tttgctcgcc
     1621 ggtgtcgcct cgacacgcct cggcctgccg ttcctgctgg tgtttctggg ggccgggatg
     1681 ctggtcggtg tcgacggccc cgtcggcgtg cgcttcgacg acgtgaagct cgcggccgcg
     1741 ctcggcaacg ctgcgctggc gatgatcctg ctcgaaggcg ggatcagcac gcggatggaa
     1801 gtctttcgcg ccggttttcg cccggcgttc gcgctcgcga cgatcggcgt cgtgctgacg
     1861 gcggcggtgg tcggcgcggc cgcgatggcg gcgctgcacg tcgactggcg ccacggcctg
     1921 ctgctcggtg cgatcgtcgg ctcgaccgat gccgccgcgg tgttcccgct gctgcggcag
     1981 accggcttgc gcctgtcgga gcgcgtcgcg gcgacgctcg agctcgaatc gggcttgaac
     2041 gacccgatgg cggtgtttct cgtgctcgcg ctgatcgcga cgctgaagac cgacgcgggc
     2101 ggcgccgagc tgctgctgct gttcgcgcgc caggccggct tcggcgcagc gatcgggctg
     2161 ggcgcgggct gggcggtcgc gtggctgctc gcgaagctgc cgttggacgt cgagcacggc
     2221 ggggtgctgt cgctgatcgt cgtgtccgcc gcgatggcgg tgttcgcgac ctgcgggctg
     2281 atcgagggat cgggctttct cgc
//
LOCUS       DASRDG010000378         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1440878, whole genome shotgun sequence.
ACCESSION   DASRDG010000378 DASRDG010000000
VERSION     DASRDG010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440878"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VFA35_06275"
     CDS             <1..1365
                     /locus_tag="VFA35_06275"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZT55812.1"
                     /translation="DALTGLPTRPYFERRLLRAAKKCDARPQTRLAVLFIDLDGFKPV
                     NDTFGHSSGDRVLAQVGRRLKARARGAGAVARLGGDEFVLLAVGRGGEDSVARLAVRV
                     IAALSQPYKVGGREVAISCSVGIAMYPDDAGACDAGKLIARADAAMYAAKRAGGARHC
                     FYVPTMDVDAQHNFDLLRDLRLALPNDELELYFQPKVDARSGKVTAAEALLRWNHPTR
                     GLVMPGDFIALAERSGLIGPLGNWVIEAACKQVRAWRDKGLRMRVAINLSAHQMRQDD
                     IAERIAAALARHRIHPALLTCEITETAAMEDTGATQETFRRLGEMGAHLSIDDFGTGY
                     SSLAYLRRLPAEELKIDASFVGDVDHSADARAVIDAVVKLAHALGLKVVAEGVETLRQ
                     QKILVELGCDELQGYLFARPMTARALLRWAMDDRANSAVFRDSLFGETRQATSGEASP
                     QTPA"
     gene            complement(1394..>2566)
                     /locus_tag="VFA35_06280"
     CDS             complement(1394..>2566)
                     /locus_tag="VFA35_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HZT55813.1"
                     /translation="EPYSAAFAGEMRVPSSSIPVMVMSERKIIARRAALELKPNSVVN
                     LGIGMPEGVASVAAEERVIDLLTLTAEPGVVGGIPAGGMSFGAAINAQAIIDQPYQFD
                     FYDGGGLDIAFLGLAQADAQGNLNVSKFGPKLAGAGGFINISQNAKKVVFVGSFNAGR
                     LSVAIEGGELRILEDGNCTKFVAEVEHRTFSGAESLRRGQRVLYITERCVFRLGARGL
                     ELIEIAPGVDLQRDILACMDFAPIIDAPPALMDAAIFRHETMGLRERMLLLPLPERFA
                     YDAQQNTLFINFEGHTVKSLADVAAIRAQVEAIVAPLGHRVQAIVNYDNFTILPEVLE
                     PYTEMVEGLVERHYSRVSRYAASNFLRVKLGDALKQRGLAPHIFERAEAARQAVAP"
BASE COUNT          397 a          893 c          900 g          376 t
ORIGIN      
        1 gacgccctga ccggcctgcc gacgcggccg tacttcgagc gccggctgct acgcgccgcg
       61 aagaagtgcg atgcgaggcc gcagacccgg ctcgcggtgc tgttcatcga cctcgacggc
      121 ttcaagccgg tcaacgacac cttcggccac agcagcggcg accgcgtgct ggcgcaggtc
      181 ggcaggcgcc tgaaggcgcg cgcgcgcggc gccggcgcgg tggcgcggct cggtggcgac
      241 gagttcgtgc tgctcgcggt cggccgcggc ggcgaggaca gcgtcgcgcg gctggcggtg
      301 cgcgtgatcg ccgcgctgag ccagccgtac aaggtcggcg gccgcgaggt cgcgatctcg
      361 tgctcggtcg gcattgcgat gtaccccgac gacgccggcg catgcgatgc gggcaagctg
      421 atcgcgcgcg ccgacgccgc gatgtacgcg gccaagcgcg ccggcggtgc acgccactgc
      481 ttctacgtgc ccacgatgga cgtcgacgcg cagcacaact tcgatctgct gcgtgacctg
      541 cgcctggcgc tgccgaacga cgaactcgag ctctacttcc agcccaaggt cgacgcgcgc
      601 agcggcaagg tcaccgcggc cgaggccctg ctgcgctgga accacccgac ccgcgggctg
      661 gtgatgccgg gcgacttcat cgcgctggcc gagcgctcgg gcctgatcgg gccgctcggc
      721 aactgggtca tcgaggccgc gtgcaagcag gtgcgcgcct ggcgcgacaa gggcctgcgc
      781 atgcgcgtgg cgatcaacct gtcggcccac cagatgcgcc aggacgacat cgccgagcgc
      841 atcgccgcgg cgctggcgcg ccaccgcatc caccccgcgc tgctgacctg cgagatcacc
      901 gagaccgcgg cgatggaaga caccggcgcg acccaggaga ccttccgccg cctcggcgag
      961 atgggcgcgc acctgtcgat cgacgatttc ggcaccggct attcgagcct cgcctacctg
     1021 cgccggctgc ccgccgaaga gctgaagatc gacgccagct tcgtgggtga cgtggaccac
     1081 agcgccgatg cccgcgccgt gatcgacgcg gtcgtcaagc tcgcgcacgc gctcgggctg
     1141 aaggtcgtgg ccgagggcgt cgagaccctg cgccagcaga agatcctggt cgagctcggc
     1201 tgcgacgaac tgcagggcta cctgttcgcc cggccgatga cggcgcgcgc gctgctgcgc
     1261 tgggcgatgg acgatcgcgc gaactcggcg gtgttccgcg actcgctgtt cggcgagaca
     1321 cggcaggcga cttcgggcga ggccagcccg cagaccccgg cctgaaacgc caccgtgccc
     1381 tgcgccgtcc cggctatggc gcgaccgcct ggcgggcagc ctcggcgcgc tcgaagatgt
     1441 gcggcgccag accgcgctgc ttcagcgcgt cgccgagctt gacgcgcagg aagttgctcg
     1501 ccgcgtaccg cgacacgcgc gaatagtgac gctcgaccag gccctcgacc atctcggtgt
     1561 agggctcgag cacctcgggc aggatcgtga aattgtcgta gttgacgatc gcctgcacgc
     1621 ggtggcccag cggcgcgacg atggcctcga cctgcgcgcg gatcgccgcg acatcggcga
     1681 gcgacttcac ggtgtggccc tcgaaattga tgaacagcgt gttctgctgc gcgtcgtagg
     1741 cgaagcgctc gggcagcggc agcagcagca tgcgctcgcg caggcccatg gtctcgtggc
     1801 gaaagatcgc cgcgtccatc agcgcgggtg gcgcatcgat gatgggcgcg aaatccatgc
     1861 acgcgaggat gtcgcgctgc agatcgacgc ccggcgcgat ctcgatcagc tcgaggcccc
     1921 gcgcgccgag gcggaacacg cagcgctcgg tgatgtagag cacgcgctgg ccgcgccgaa
     1981 ggctctcggc gccgctgaag gtgcggtgct cgacctcggc gacgaacttc gtgcagttgc
     2041 cgtcttcgag gatgcgcagc tcgccgccct cgatcgccac gctcagccgt ccggcattga
     2101 agctgccgac gaacacgacc ttcttcgcgt tctggctgat gttgatgaag ccgccggcgc
     2161 cggccagctt cgggccgaac ttgctgacgt tcaggttgcc ctgcgcgtcg gcctgggcta
     2221 ggccgaggaa ggcgatgtcg aggccaccgc cgtcgtagaa gtcgaactgg tagggctggt
     2281 cgatgatcgc ctgcgcgttt atagccgcgc cgaaactcat accgccggcg ggaattccgc
     2341 ccacaacgcc cggctccgca gtgagcgtga gaagatcaat gacccgttcc tccgcggcga
     2401 cgctggctac gccttcaggc atgccgatgc cgagattgac gacactgttc ggcttcaact
     2461 ccagcgcggc gcggcgcgcg atgatcttgc gctcggacat caccatgacc gggatcgagg
     2521 acgagggcac tcgcatctcg ccggcgaacg ccgcgctata cggctc
//
LOCUS       DASRDG010000379         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1569676, whole genome shotgun sequence.
ACCESSION   DASRDG010000379 DASRDG010000000
VERSION     DASRDG010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569676"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFA35_06285"
     CDS             <1..474
                     /locus_tag="VFA35_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZT55814.1"
                     /translation="GSRGSPANPILQGQHPEYLVKQLTEFKSGKRQNAVMSGMAATLS
                     EDDMKNVAAFYAGKTAKAGFAKNKATVELGEKIYRGGIAERNVPACAGCHSPNGAGLP
                     AQYPRLGGQHADYTEAQLVAFRSGARKNSVPMSGVAAKMNDREIKAVADYIAGLH"
     gene            569..>2291
                     /locus_tag="VFA35_06290"
     CDS             569..>2291
                     /locus_tag="VFA35_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HZT55815.1"
                     /translation="MSATTAGIEVRAQSRWVRDGVELVSSMRFSISLLTVICIASVIG
                     TVVKQHEPYNNYVNQFGPFWAELFGKVGLYTVYSAWWFLLILAFLVLSTSLCIARNVP
                     KIIHDLKTYKEQVREQALASFHHRGSSEVAESREQALERIGALLVHGGWKAKVQVREN
                     GTMIAARRGTANKLGYLAAHSAIVLVCVGGLLDGDLIVRAQMLLQGKHPYAGGGLMKD
                     VPVQYRLGPGTPTFRANLFVPEGARAGTAVINMQDGVVLQDLPFDVELKKFIVDYYET
                     GMPKLFASEIVIHDRDTGAATAALVKVNEPAFHRGVAIYQSSFDDGGSSLKLRGIPMR
                     AGGRPFEVDGVVGGSAQISNGDTKMTLEFTGLRVINVENLGGGAATDVRKVDLVGSLK
                     NHLGSGAKGNGKKDLRNVGPSVSYKLRDAAGQAREFNNYMLPVELDGQRVFLAGTRDK
                     PDQPMRYLRIPADDKDSIDGWVRLRAALLDPALRAKAAARYVALATPADKPEMAAQLT
                     TTAARALALFAGAESARTKDNADPSRPVGGLQAVSDFIESSVPAPDRGRISEVLLRIL
                     NGSLFELL"
BASE COUNT          393 a          801 c          770 g          327 t
ORIGIN      
        1 ggctcacgcg gcagccccgc gaacccgatc ctccagggcc agcaccccga gtacctcgtc
       61 aagcagctga ccgagttcaa gtcgggtaag cgccagaacg ccgtgatgtc ggggatggcc
      121 gcgacgctca gcgaagacga catgaagaac gtcgccgcgt tctacgccgg caagaccgca
      181 aaggcgggct tcgcgaagaa caaggccacg gtcgagctcg gcgagaagat ctaccgcggc
      241 ggcatcgccg agcgcaacgt gccggcctgt gccggctgcc acagcccgaa cggcgcaggc
      301 ctgccggcgc agtacccgcg cctgggcggc cagcatgccg actacaccga ggcgcaactg
      361 gttgctttcc gcagcggcgc acgcaagaac agcgtgccga tgagcggcgt cgccgcgaag
      421 atgaacgatc gcgagatcaa ggccgtggcc gactacatcg ccggcctgca ctgaaccact
      481 cggcgatcgc tcggcgaaag ggctggcctg gcgccagccc tttctgtttc cgcccctcgc
      541 gtccacgccc cgcccggata atctccccat gagcgccacc accgccggca tcgaagtgcg
      601 cgcgcaatcg cgctgggtgc gcgacggcgt cgagttggtg tcgtcgatgc ggttctcgat
      661 ctcgctgctg accgtcatct gcatcgcttc ggtgatcggc acggtcgtca agcagcacga
      721 gccgtacaac aactacgtca accagttcgg accgttctgg gccgaattgt tcggcaaggt
      781 cgggctctac acggtctaca gcgcctggtg gttcctgctg atcctggcgt ttctggtcct
      841 ctcgaccagc ctgtgcatcg cgcgcaacgt gccgaagatc atccatgacc tgaagaccta
      901 caaggagcag gtgcgcgagc aggccctggc gtcgttccac caccgcggca gctcggaagt
      961 ggccgagtcg cgcgagcagg cgctcgaacg catcggcgcg ctgctggtgc atggcggctg
     1021 gaaggccaag gtgcaggttc gcgagaacgg cacgatgatc gctgcgcggc gcggcacggc
     1081 caacaagctc ggctacctcg cggcgcactc ggcgatcgtg ctggtgtgcg tcggcggctt
     1141 gctcgacggc gacctgatcg tgcgcgcgca gatgctgctg cagggcaagc acccctacgc
     1201 cggcggcggc ctgatgaagg acgtgccggt gcaataccgt ctcggccccg gcacgccgac
     1261 cttccgcgcc aacctgttcg tgcccgaggg cgcgcgcgcc ggcaccgcgg tgatcaacat
     1321 gcaggacggc gtcgtgctgc aggacctgcc gttcgacgtc gaactcaaga agttcatcgt
     1381 cgactattac gagaccggca tgcccaagct gttcgccagc gagatcgtga ttcacgaccg
     1441 cgacaccggc gcggcgacag cggcgctggt caaggtcaac gagccggcgt tccaccgtgg
     1501 cgtggcgatc taccagagca gcttcgacga cggcggttcg tcgctgaagc tgcgcggtat
     1561 cccgatgcgc gccggcggcc ggccgttcga ggtcgacggc gtggtcggcg gcagcgcgca
     1621 gatcagcaac ggcgacacga agatgacgct cgagttcacc gggctgcgcg tgatcaacgt
     1681 cgagaacctc ggcggcgggg ccgcgaccga cgtgcgcaag gtcgacctgg tcggctcgct
     1741 gaaaaatcat ctcggctcgg gcgcgaaggg caacggcaag aaggatctgc gcaacgtcgg
     1801 cccgtcggtc agctacaagc tgcgcgacgc cgccggccag gcgcgcgagt tcaacaacta
     1861 catgctgccg gtcgagctcg acggccagcg cgtgttcctc gccggcacgc gcgacaagcc
     1921 cgaccagccg atgcgctacc tgcgcatccc cgccgacgac aaggacagca tcgacggctg
     1981 ggtccgcctg cgcgccgcgc tgctcgaccc ggccctgcgg gcgaaggccg cggcccgcta
     2041 cgtggcgctc gccacgccgg ccgacaagcc cgagatggct gcgcagctca caaccaccgc
     2101 cgcgcgcgcg ctcgcgctct tcgcgggcgc cgagagcgcg cgcaccaagg acaacgcgga
     2161 tccgtcccgg ccggtcggcg ggctgcaggc cgtctcggac ttcatcgaga gcagcgtgcc
     2221 cgcgcccgac cgcggccgca tctccgaggt gctgctgcgc atcctgaacg gcagcctgtt
     2281 cgagctgctg a
//
LOCUS       DASRDG010000380         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1569911, whole genome shotgun sequence.
ACCESSION   DASRDG010000380 DASRDG010000000
VERSION     DASRDG010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569911"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2062
                     /locus_tag="VFA35_06295"
     CDS             <1..2062
                     /locus_tag="VFA35_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZT55816.1"
                     /translation="LGVIGWVLGAPQGMPALVVDGQALSVVERSGEAVGVGLFLVAAA
                     MFARRLRTPQPFDVASLLCAALLMALGEGVLALDAQLGGLAQIVGHVYKVLGFWMVYH
                     AVVADSIRSPYQRLLDSRRRVRHSEEELRTITDNIPAMVAFVDSEQRYRFVNRNYQRW
                     LGLKPETMLGRTALEVYGEEHYGHLQPHIERALAGQTFAVDHRVTGAECARGRWLNIK
                     YMPKVGPDGAVSGVYILSSDITDVMVAERRALFLAEHDELTTLPNRAAFQVAVGKALE
                     HSAREMSQFAVIFVDVDRFKVVNDTLGHAAGDQLLQALAQRMKQVVRGQDLVARMGGD
                     EMCLLLHNVRDAAHASEIAERILGALRAPIELRPGMQHHVTVSAGIAIFPQDGTDTET
                     LLRHADIAMYRAKAAGRDGFALFGAHDGGPAEARLTLELELRTAIDAEQLVLHYQPRV
                     DVITGRAVGVEALVRWQHPERGLLAPGHFIDIAEETGLIVPMGAWVLCQAGLALKRLQ
                     AAGAGALTMSVNVSALQFKNPKLVDEVAGMLRLAGCAPHQIELELTESAVMDQPHQAE
                     ATMHRLKTLGVRLAMDDFGTGHSSLGALKRFPVDCVKIDRSFVSDIPGNAGDAALTRA
                     IIAMGHSLELQVVAEGVERQDQLDFLRREGCDEYQGYFFAKPMPEAALTTLLTQTPRA
                     ALVA"
     gene            2086..>3241
                     /locus_tag="VFA35_06300"
     CDS             2086..>3241
                     /locus_tag="VFA35_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55817.1"
                     /translation="MPLRALLRHLLLAALLTALLPAAPALAAADADPRILALEKLGRA
                     QPFEAAKALDALLPEVPEHSAQRLELLTVQGLMLAAAAPPEAVEPTVARLDAWGRDPT
                     AANAAAAAAAALLVRARSVARDGNLHSADLLMREAMARLPADTAGRARYRFVFVHGYI
                     LGRAGQLEPAVRLSHEALGLADAHGELWQQSEARTELANSYHAARQLERARSLSAEAI
                     ALAAKADDAVALGRAHNTAAIVLDALGDPPGERRSLEAAIASARRAGAQLDEVRYLAN
                     LADFFLKNGEYKTALATAERALPLARALKDRNSEMVALANIGLAHISLLHLEPGKRYV
                     REAIAIDEGRGAVSGVADTLRELGTYLEKAGDAAGAVAAYHQHRLLATTLL"
BASE COUNT          461 a         1189 c         1173 g          418 t
ORIGIN      
        1 gctgggtgtg atcggctggg tgctcggcgc gccgcagggc atgccggcgc tggtggtcga
       61 cggccaggcg ctcagcgtcg tcgagcgttc cggcgaggcg gtgggggtcg gcctgttcct
      121 ggtcgccgcg gcgatgttcg cgcggcgcct gcgcacgccg cagcccttcg acgtggcgtc
      181 gctgctgtgc gccgcgctgc tgatggcgct cggcgagggc gtgctggcgc tggacgcgca
      241 gctgggcggc ctggcgcaga tcgtcggcca cgtctacaag gtgctgggct tctggatggt
      301 gtaccacgcg gtcgtcgccg acagcatccg ctcgccctac cagcgcctgc tggattcgcg
      361 ccgccgggtg cgccacagcg aggaagagct gcgcacgatc accgacaaca tcccggcgat
      421 ggtcgcgttc gtcgacagcg agcagcgcta ccgcttcgtc aaccgcaact accagcgctg
      481 gctcggcctg aagcccgaga cgatgctcgg ccgcaccgcg ctcgaggtct acggcgagga
      541 gcactacggg cacctgcagc cgcacatcga gcgcgcgctc gcgggccaga ccttcgcggt
      601 cgaccaccgc gtcaccggcg ccgagtgcgc gcgcgggcgc tggctcaaca tcaagtacat
      661 gcccaaggtc ggccccgacg gcgcggtcag cggcgtctac atcctgagca gcgacatcac
      721 cgacgtgatg gtggccgaac gccgcgcgct gttcctggcc gagcacgacg agctgacgac
      781 gctgccgaac cgcgccgcgt tccaggtcgc ggtcggcaag gcgctcgagc acagcgcgcg
      841 cgagatgagc cagttcgcgg tgatcttcgt cgacgtcgac cgcttcaagg tcgtcaacga
      901 cacgctcggc cacgccgccg gcgaccaact gctacaggcg ctggcgcagc gcatgaagca
      961 ggtcgtgcgc gggcaggacc tggtcgcgcg catgggcggc gacgagatgt gcctgctgct
     1021 gcacaacgtg cgcgacgccg cgcatgcgag cgagatcgcc gagcgcattc tcggcgcgct
     1081 gcgcgcgccg atcgagctgc gccccggcat gcagcaccac gtgacggtct cggccggcat
     1141 cgcgatcttt ccgcaggacg gcaccgacac cgagacgctg ctgcgccatg ccgacatcgc
     1201 gatgtaccgc gccaaggcgg ccggccgcga cggcttcgcg ctgttcggcg cgcacgacgg
     1261 cggcccggcc gaggcgcgcc tgaccctcga actcgagctg cgcaccgcga tcgatgcaga
     1321 gcaactggtg ctgcactacc agccgcgcgt cgacgtcatc acgggccggg cggtgggcgt
     1381 cgaagcgctg gtgcgctggc agcaccccga acgcgggctg ctcgcgcccg gccacttcat
     1441 cgacatcgcc gaagagaccg gcctgatcgt gccgatgggc gcgtgggtgc tctgccaggc
     1501 cggcctggcg ctcaagcgcc tgcaggcggc aggcgcgggt gcgctgacga tgtcggtcaa
     1561 cgtcagcgcg ctgcagttca agaacccgaa gctggtcgac gaggtcgccg gcatgctccg
     1621 gctggccggc tgcgcgccgc accagatcga gcttgagctc accgagagcg cggtgatgga
     1681 ccagccgcac caggccgagg ccacgatgca ccgcctgaag acgctcggcg tgcgcctggc
     1741 gatggacgac ttcggcaccg gccactcgtc gctcggcgcg ctgaagcgct ttccggtcga
     1801 ctgcgtgaag atcgaccgct cgttcgtcag cgacatcccc ggcaacgccg gcgacgcggc
     1861 gctgacccgc gcgatcatcg cgatgggcca ctcgctcgaa ctgcaggtgg tggccgaggg
     1921 cgtggagcgg caggaccagc tcgacttcct gcgtcgcgaa ggctgcgacg aataccaggg
     1981 ctacttcttc gccaaaccga tgcccgaggc cgcgctgacg acgctgctga cgcagacgcc
     2041 gcgcgcggcg ctggtcgcct gacgtccgac gccggagcga cgacgatgcc gttgcgcgcg
     2101 ctgctccgcc acctgctgct cgccgctttg ctgaccgcgc tgctgccggc cgcgccggcg
     2161 ctggccgcgg ccgacgccga cccgcgcatc ttggcgctgg agaagctcgg ccgcgcccag
     2221 ccgttcgagg ccgcgaaggc gctcgacgcg ctgctgcccg aagtgcccga gcacagcgcg
     2281 cagcggctcg agctgctcac tgtgcagggc ctgatgctgg cggccgcggc gccgcccgag
     2341 gcggtggaac ccacggtggc gcgcctcgac gcctggggcc gcgacccgac ggcggcaaac
     2401 gctgcggcgg ccgcggccgc ggcgctgctg gtgcgcgcgc gctcggtggc ccgcgacggc
     2461 aacctgcaca gcgccgacct gctgatgcgc gaggcgatgg cccggctgcc cgccgacacc
     2521 gcggggcgcg cgcgctaccg cttcgtcttc gtgcacggct acatcctcgg gcgcgccggc
     2581 cagctcgaac cggcagtgcg gctgagccac gaggcgctgg ggctggccga cgcgcacggc
     2641 gagctctggc agcagtcgga ggcgcgcacc gagctggcca acagctacca cgccgcgcgg
     2701 cagctcgagc gcgcgcgcag cctgagcgcc gaggcgatcg cgctcgccgc gaaggccgac
     2761 gacgcggtcg cgctcggccg cgcgcacaac accgccgcga tcgtgctcga cgcgctgggc
     2821 gacccgcccg gcgagcggcg ctcgctcgag gccgcgatcg cgagcgcgcg gcgcgccggc
     2881 gcccagctcg acgaagtgcg ctacctcgcg aacctggccg acttcttcct gaagaacggc
     2941 gaatacaaga ccgcgctcgc caccgccgag cgcgcgctgc cgctggcgcg cgcgctgaag
     3001 gaccgcaaca gcgagatggt cgcgctcgcc aacatcggcc tggcgcacat ctcgctgctg
     3061 caccttgagc cgggcaagcg ctacgtgcgc gaggcgatcg cgatcgacga agggcgcggc
     3121 gcggtgagcg gcgtggccga caccttgcgc gagctcggca cctacctcga aaaggccggc
     3181 gacgcggccg gcgcggtggc tgcctaccac cagcaccggc ttcttgcgac cacgctgctg
     3241 c
//
LOCUS       DASRDG010000381         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1319334, whole genome shotgun sequence.
ACCESSION   DASRDG010000381 DASRDG010000000
VERSION     DASRDG010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319334"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFA35_06305"
     CDS             <1..285
                     /locus_tag="VFA35_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55818.1"
                     /translation="VFAVIALAACLVLLLRLVLGAPRRRRFDATVRRAALGLRRRGHA
                     IWHWREARRDAERARREADEAIRRARDRGAWDGNVYTPKSFRKPPRDKTH"
     gene            complement(308..1630)
                     /locus_tag="VFA35_06310"
     CDS             complement(308..1630)
                     /locus_tag="VFA35_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZT55819.1"
                     /translation="MNTLIIFVLLLALMLTGMPISISLGLTVLTFLFTLTQVPIESVA
                     LKLFTGIEKFEVMAIPFFILAGNFLTHGGVAKRMINFCTSMIGHWYGGMGLAGVMACA
                     LFAAISGSSVATVVAVGSIMLPAMVKAGYPKRFGAGVITTSGALGILFPPSINLVMYA
                     IATAGMNATGPNGEPVSSASVGQLFLAGIVPGLCLSFLLGVTTWYRAKKFDYPRLPRA
                     SWAERYRAFRESMWGILLIVVVIGGIYSGMFTPTEAAAMAAVYSFFISVFVYKDLKLK
                     EVPKSLLSAAAMSSMLLYIVTNAVLFSFLMTSEQIPQAMAAWMTSQGFGLVVFLLLVN
                     VILLAAGNFMDPAAIVLIMAPILFPVAVKLGVDPVHFGILMAVNMEVGLCHPPVGLNL
                     YVASGITKMGITELTVAVGPWLLTMICFLVVVTYWPTLSLWLPHLMFK"
     gene            complement(1627..2259)
                     /locus_tag="VFA35_06315"
     CDS             complement(1627..2259)
                     /locus_tag="VFA35_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZT55820.1"
                     /translation="MKILDRLEEILIASLMAAATLLIFVAVVHRYGTGVPFLYPYFIQ
                     IHLSWAQELCIYMFVWMAKFGAAYGVRHGTHVGVDVLVRALPKDKAKYLVMFSLLAGA
                     LFTGIVGTLGARFVWENGFHYALLHNWLGMDVGDLPEGPTSPDLEIPTWIPYACVPLG
                     SYLMCFRFLQVAWAFWKTGEVPHADHGVRGVDDAAIEDLVPAADSNGGRQ"
BASE COUNT          430 a          763 c          761 g          318 t
ORIGIN      
        1 gtgttcgccg tgatcgctct cgccgcctgc ctcgtgctgc tgctgcggct cgtgctcggc
       61 gcgccgcggc gccggcgctt cgacgccacg gtgcgccgcg ccgcgctcgg cctgcgccgc
      121 cgcggccatg cgatctggca ctggcgcgag gcgcggcgcg acgccgagcg cgcgcgccgc
      181 gaagccgacg aggcgatccg gcgcgcccgc gaccgcggcg cgtgggacgg caatgtctac
      241 acgccgaagt cgttccgcaa gccgccgcgg gacaagacgc attgagcgtc cccaccgcct
      301 ggcggcccta cttgaacatc aggtgcggca gccacagcga cagcgtgggc cagtaggtca
      361 cgaccacgag gaagcagatc atcgtcagca gccacggccc gaccgcgacc gtgagctcgg
      421 tgatgcccat cttcgtgatg cccgaggcca cgtacaggtt caggccgacc ggcgggtggc
      481 acaggccgac ctccatgttg accgccatca ggatgccgaa gtgcaccggg tcgacgccga
      541 gcttgacggc caccgggaac aggatcggcg ccatgatcaa cacgatcgcg gccgggtcca
      601 tgaagttgcc ggcggccagc aggatcacgt tgacgagcag caggaacacc accaggccga
      661 agccctggct ggtcatccat gccgccatcg cctgcgggat ctgctcgctc gtcatcagga
      721 agctgaacag caccgcgttg gtcacgatgt acagcagcat cgagctcatc gcggcggcgc
      781 tgagcagcga cttcggcacc tccttcagct tcaggtcctt gtagacgaag accgagatga
      841 agaacgagta cacggccgcc atcgccgccg cttcggtcgg cgtgaacatg ccgctgtaga
      901 tgccgccgat cacgacgacg atcagcagga tgccccacat gctctcgcgg aacgcgcggt
      961 agcgctcggc ccagctggcg cgcggcaggc gcgggtagtc gaacttcttc gcgcggtacc
     1021 aggtcgtcac gccgagcagg aacgacaggc acagccccgg cacgatgccg gcgaggaaga
     1081 gctggccgac cgaggccgaa ctgaccggct cgccgttcgg cccggtcgcg ttcatgccgg
     1141 cggtcgcgat cgcgtacatc accaggttga tcgacggcgg gaacaggatg cccaaggcgc
     1201 ccgaggtcgt gatcacgccg gcgccgaagc gcttcgggta gcccgccttc accatggccg
     1261 gcagcatgat cgagccgacc gcgacgacgg tcgcgaccga cgaacccgag atcgccgcga
     1321 acagcgcgca ggccatcacg cccgcgagcc ccatgccgcc gtaccagtgg ccgatcatcg
     1381 aggtgcagaa gttgatcatc cgtttggcga cccccccgtg cgtcaggaag ttgccggcga
     1441 ggatgaagaa cgggatcgcc atgacctcga acttctcgat gcccgtgaac agcttcagcg
     1501 ccaccgattc gatcggcacc tgcgtgagcg tgaacaggaa ggtgagaacg gtcaggccga
     1561 gcgagatgct gatcggcatg ccggtcagca tcagcgccag cagcaggacg aagatgatca
     1621 gcgtgttcat tggcggcctc cgttcgagtc ggcagcgggg accaggtcct cgatcgcagc
     1681 gtcgtcgacg ccccgcacgc catggtcggc atgcggcacc tcgccggtct tccagaacgc
     1741 ccacgcgacc tgcaggaagc ggaagcacat caggtacgag cccagcggca cgcaggcgta
     1801 ggggatccag gtcgggatct cgaggtcggg cgaggtcggg ccttcgggca ggtcacccac
     1861 gtccatgccg agccagttgt gcagcagcgc gtagtggaag ccgttctccc agacgaagcg
     1921 cgcgcccagc gtgccgacga tgccggtgaa cagcgcgccg gcgagcaggc tgaacatgac
     1981 caggtacttg gccttgtctt tcggcaaggc gcgcaccagc acgtcgacgc cgacgtgggt
     2041 gccgtggcgc accccgtagg cggcgccgaa cttggccatc cacacgaaca tgtagatgca
     2101 cagctcctgc gcccacgaca ggtgaatctg gatgaaatag gggtagagga acggcacgcc
     2161 ggtgccgtag cgatgcacga ccgcgacaaa aatcagcagc gtcgcagccg ccatcagcga
     2221 tgcgatcaaa atttcctcga gccggtcgag gattttcagg aagatttgca gg
//
LOCUS       DASRDG010000382         4333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1091256, whole genome shotgun sequence.
ACCESSION   DASRDG010000382 DASRDG010000000
VERSION     DASRDG010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091256"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2795
                     /locus_tag="VFA35_06320"
     CDS             <1..2795
                     /locus_tag="VFA35_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HZT55821.1"
                     /translation="ADYDIAMGLDGHKVEEGLPLQYLIKQARDERTASDEVIRIVNAR
                     EHNLKSLDVSIPRGKFSVVTGVSGSGKSTLAFDILFNEGQRRYLESLNAYARSIVQPA
                     GRPEVDAVYGIPPTVAIEQRLSRGGRKSTVATTTEVWHFLRLLYVKLGLQHCVNDGSP
                     VKPQSAESIAAQLLRDHNGEHVGLLAPLVVNRKGVYTDLAKWAKGRGHTHLRVDGEFL
                     KVDPWPRLDRFKEHTLELPVGDIVIDPANEAELRALLASALELGKGVMHLLAPLDGLI
                     GAMANETSTARIGSVKVFSTKRACPSCGTSYPELDPRMFSYNSKHGWCTTCVGTGLAL
                     TREQRKAYDDSIPDDDNKGREQSFPSEEAEVEGVVDQPCPDCHGTRLNPASRLVTFEQ
                     EPITTIGSWSVADARLWVDALQLAGRDAEIARDVVSEIRSRLEFLEEVGLGYLTLDRA
                     APTLSGGEAQRIRLAAQLGSNLQGVCYVLDEPTIGLHPRDNQVLLGALQTLSDKGNTL
                     VVVEHDEDTIRRADHIIDIGPGAGKRGGNLVAEGSASEISSNPESVTGRFLAHPLIHP
                     LQARRFVEPVTAATVRKESEANRLLTIKGATLHNLRDVTVDLPLKRLVAVTGVSGSGK
                     STLARDVLLANLQFINTRGGKPNWQGCEGIDGWGYIDRVLEVDQTPIGKTPRSCPATY
                     IGFWDTIRKLFADTLEARARGYAPARFSFNTGEGRCPGCEGQGMKTIAMSFLPDVKVP
                     CDVCHGARFNAETLAVSWRGKSIGDVLTMEVDEAVEFFTSMPHIAHPLQLLKDVGLGY
                     LTLGQPSPTLSGGEAQRIKLVTELSKVRDDVTRRGNKAPHTLYVLDEPTVGLHMADVE
                     RLIRVLHRLVDGGHSVIVIEHDLDVIAEADWVIDLGPEGGVKGGEVVGESTPEALVGN
                     ARSHTGAALRPVLARG"
     gene            2909..3784
                     /locus_tag="VFA35_06325"
     CDS             2909..3784
                     /locus_tag="VFA35_06325"
                     /inference="COORDINATES: protein motif:HMM:NF027261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4747 family protein"
                     /protein_id="HZT55822.1"
                     /translation="MPRPASFDVGCINIRVHPDHTTQRYLQFWRALHALRRPLVRRSG
                     NALMIGEVQRPNADARYVVGEFYRFIQINEDDQWFNIDAHKPAEADELDAISIPDELR
                     PNLQFVPYVLDLKRHRLYVSTRSSDASLGIASVLKLLQSLVNHQKIHSQFGQVDLTVA
                     SDVADVDRMLSLPVLRSVQLVIERPNASDYDDERVVLDHLESIGASKETRQYVKAPNE
                     QTLRPDDDMRALARVAASNGAVKVTGFDAARRPVKASSSSFPWFMRSTYNSNSQTLFQ
                     AFVSFVEDISSRRIP"
     gene            3781..>4333
                     /locus_tag="VFA35_06330"
     CDS             3781..>4333
                     /locus_tag="VFA35_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55823.1"
                     /translation="MSRPSPLRTLGVLAFVWGMGGGLKGALRSFDFWLALVVWLLSIG
                     LWTTPKWTDTVISVLPNLLGFTLGGYTVFLGFGSDSFKGAITAESERDSVYLSVSSAF
                     MLFVGVQLFALLYALVAASLYFTTPNCLLSFSVELGHAAKAGGAIGYFIFCYSIALAL
                     RAAVRIFRLSRWYNTYLARTRQTL"
BASE COUNT          816 a         1397 c         1390 g          730 t
ORIGIN      
        1 tggccgacta cgacatcgcg atggggctgg acggccacaa ggtcgaggaa ggcttgccgc
       61 tgcagtacct gatcaagcag gcgcgcgacg agcggaccgc gagcgacgag gtcatccgca
      121 tcgtcaacgc ccgcgagcac aacctgaagt cgctcgacgt cagcatcccg cgcggcaagt
      181 tcagcgtcgt caccggcgtc tcgggctcgg gcaagtcgac gctcgcgttc gacatcctgt
      241 tcaacgaagg ccagcgccgc tacctcgaat cgctgaacgc gtatgcgcgc agcatcgtgc
      301 agccggccgg gcggcccgag gtcgacgcgg tctacggcat cccgccgacc gtcgcgatcg
      361 agcagcggct gtcgcgcggc gggcgcaaga gcacggtcgc gaccaccacc gaggtctggc
      421 acttcctgcg gctgctgtac gtgaagctcg gcttgcagca ctgtgtcaac gacggttcgc
      481 cggtgaagcc gcagagcgcc gagagcattg cggcgcaact gctgcgcgac cacaacggtg
      541 agcatgtcgg cctgctcgcg ccgctggtcg tcaaccgcaa gggcgtctac accgacctcg
      601 cgaagtgggc gaaggggcgc gggcacacgc acctgcgcgt cgacggcgag tttctgaagg
      661 tcgacccgtg gccgcggctc gaccgcttca aggagcacac gctcgaactg ccggtcggcg
      721 acatcgtgat cgaccccgcg aacgaagccg agctgcgcgc gctgctggcg agcgcgctcg
      781 aactcggcaa gggcgtgatg cacctgctcg ccccgctcga cgggctgatc ggcgcgatgg
      841 cgaacgagac ctcgaccgcg cgcatcggca gcgtgaaagt cttctcgacc aagcgcgcgt
      901 gcccgagctg cggcaccagc taccccgagc tcgacccgcg catgttcagc tacaacagca
      961 agcacggctg gtgcacgacc tgcgtcggca ccggcctcgc gctgacgcgc gagcagcgca
     1021 aggcctacga cgattcgatc ccggacgatg acaacaaggg ccgcgagcaa tcgttcccgt
     1081 ccgaggaggc cgaggtcgaa ggcgtcgtcg accagccgtg ccccgactgc cacggtacgc
     1141 ggctgaaccc ggcctcgcgg ctcgtcacct tcgagcagga gccgatcacg acgatcgggt
     1201 cgtggtcggt cgccgatgcg cggctctggg tcgatgccct gcaactcgcc ggccgcgacg
     1261 ccgagatcgc gcgcgacgtc gtcagcgaga tcaggagccg gctcgagttc ctcgaagaag
     1321 tcggcctcgg ctacctgacg ctcgaccgcg cggcgccgac cttgagcggc ggcgaggccc
     1381 agcgcatccg cctcgcggcg cagctcggca gcaacctgca gggcgtgtgc tacgtgctcg
     1441 acgagccgac catcggcctg cacccgcgcg acaaccaggt gctgctgggc gcgctgcaga
     1501 cgctcagcga caaaggcaac acgctggtcg tcgtcgagca cgacgaagac acgatccgcc
     1561 gcgccgacca catcatcgac atcggccccg gcgccggcaa gcgcggcggc aacctggtcg
     1621 ccgaaggcag cgcgagcgag atcagcagca accccgagtc ggtgaccggc cgcttcctcg
     1681 cgcacccgct gatccacccg ctgcaggcgc gccgcttcgt cgagccggtg acggccgcga
     1741 cggtgcgcaa ggagagcgaa gcgaaccgcc tgctgacgat caaaggcgcg acgctgcaca
     1801 acctgcgcga cgtgacggtc gacctgccgc tgaagcgcct ggtcgccgtc accggcgtca
     1861 gcggctccgg caagagcacg ctcgcgcgcg acgtgctgct cgccaacctg cagttcatca
     1921 acacgcgcgg cggcaagccg aactggcagg gctgcgaggg catcgacggc tggggctaca
     1981 tcgaccgcgt gctcgaagtc gaccagacgc cgatcggcaa gacgccgcgc tcctgcccgg
     2041 cgacctacat cggcttctgg gacacgatcc gcaagctctt cgccgacacg ctcgaagcgc
     2101 gcgcacgcgg ctacgcgccg gcgcgcttca gcttcaacac cggcgaaggc cgctgcccgg
     2161 gctgcgaagg gcagggcatg aagacgatcg cgatgagctt cctgcccgac gtgaaggtac
     2221 cgtgcgacgt ctgccacggc gcgcgcttca atgccgagac gctggcggtg agctggcggg
     2281 gaaaaagcat cggcgacgtg ctgacgatgg aggtcgacga ggccgtcgag ttcttcacca
     2341 gcatgcccca catcgcgcac ccgctgcagc tgctgaagga cgtgggcctg ggctacctga
     2401 cgctcggcca gccgtcaccg acgctgagcg gcggcgaggc ccagcgcatc aagctcgtca
     2461 ccgagctcag caaggtgcgc gacgacgtca cgcgccgcgg caacaaggcg ccgcatacgc
     2521 tgtacgtgct cgacgagccg accgtcggcc tgcacatggc cgatgtcgag cgcctgatcc
     2581 gcgtgctgca ccgcctcgtc gacggcggcc acagcgtcat cgtgatcgag cacgacctcg
     2641 acgtgatcgc cgaagccgac tgggtcatcg acctcgggcc cgaaggcggt gtgaagggcg
     2701 gcgaggtggt cggggagagc acgccggagg cgttggtggg gaatgcgcgg agccacacgg
     2761 gggcggcgct taggccggta ttggcccgag ggtgactgcc ggcgcaatcc ccaattcgcg
     2821 tctgcgcgat tgtatgtaac gtctggcaac atcctgtccg ctgatcgggc atgtgcaccg
     2881 accaaataaa cagacaagag gagcaagcat gccgcgtccc gcgagtttcg atgttggttg
     2941 catcaacatt cgggtgcatc ccgaccatac gacgcagcgc tatctgcagt tctggcgagc
     3001 gctccacgcg ttgcggaggc cgctggtcag gcgctccgga aacgctttaa tgattggcga
     3061 agtacagcgg cccaacgctg atgctagata cgtcgtcggg gagttctatc gcttcattca
     3121 gataaatgag gacgaccagt ggttcaacat agatgcgcac aagcctgcag aggctgacga
     3181 gctcgacgcg atttcgattc cagatgagtt gcgcccgaac ctgcaattcg ttccttacgt
     3241 tcttgatctg aagagacatc gcttgtacgt atcgacgcga tcgtcagacg cttcgctagg
     3301 catagcctct gtgttgaaac tccttcagtc gctggtgaac caccagaaga ttcactcgca
     3361 gttcgggcag gtcgacctca ctgttgcgtc ggacgtggcg gatgtcgata ggatgctttc
     3421 gttgcccgta ctaaggagcg ttcagcttgt catagagcgg ccgaacgcat cagactacga
     3481 cgacgagcgg gttgttttgg accatcttga gtccatagga gcaagcaagg agactcggca
     3541 atacgtcaag gctccaaatg aacagacact gcgaccagac gatgacatgc gcgcgctcgc
     3601 gagagtggct gcgagcaatg gtgcggtgaa ggtgaccggc ttcgatgccg ctcgaaggcc
     3661 tgtaaaggct agttcaagta gcttcccttg gttcatgcga tcgacataca acagcaactc
     3721 tcagacgttg tttcaagcgt tcgtcagctt cgtagaagac atttcgagcc gccgaattcc
     3781 atgagcagac cgtccccgct cagaacgcta ggcgttcttg ccttcgtatg gggcatgggc
     3841 ggaggactca agggcgcttt gcgctctttt gatttctggt tggcattggt tgtctggttg
     3901 ctgtctatag gcctttggac aactccgaag tggaccgaca cggtgatctc cgtactcccc
     3961 aatctcctcg gcttcacgtt gggcggctat acggtattcc tcggcttcgg aagcgatagc
     4021 ttcaagggcg caataacagc ggagtccgag agggactccg tctatctaag cgtgagttcg
     4081 gcgttcatgc tgtttgttgg tgtgcaactg ttcgcccttc tatacgcgct tgtcgctgcg
     4141 tcgctctact tcactacgcc aaattgtttg cttagcttct cggtcgaact aggacacgca
     4201 gcgaaagcgg gcggcgcaat tggctacttc atcttttgct acagcatcgc acttgcactt
     4261 cgggccgccg tgcgaatttt caggctctct cgctggtaca acacttacct cgcccggaca
     4321 aggcaaacac taa
//
LOCUS       DASRDG010000383         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1210672, whole genome shotgun sequence.
ACCESSION   DASRDG010000383 DASRDG010000000
VERSION     DASRDG010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210672"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VFA35_06335"
     CDS             complement(<1..1435)
                     /locus_tag="VFA35_06335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZT55824.1"
                     /translation="MKSPKDRQEVDKCGPQRALEKLGLVRPIDFALHLPMRYEDETRL
                     LPIASLRDGSVGQVEGMVRDCEVQIRVRRQLVVRIHDGSDDLVLRFLHFYPSHQKALA
                     VGTRVRVRGEARGGFFGLEMVHPTFKAVAEGAPLATALTPVYPTSAQLPQAYLRKAIE
                     ANLARTDLAELLPPDAVPTGMMGLRDALLFLHHPAPNVGVKALEDRSHPAWQRLKFEE
                     LLAQQLSQLQAKRERELQRAPRFGVRAGGLQEQLLAALPFRLTAAQRRVVDEIAADLQ
                     REVPMHRLLQGDVGSGKTVVAALAAAIAIDAGWQCALMAPTEILAEQHLRKLVGWLEP
                     LGITVAWLTGSRKGKARSKMLEQVASGEAMLVVGTHAVIQEQVQFARLGLAIVDEQHR
                     FGVAQRLALRAKLHDQGALEPHLLMMSATPIPRTLAMTLFADLDVSTIDELPPGRTPV
                     VTKLFVDARRDEVIARIRDESARGRQTY"
     gene            1528..>2053
                     /locus_tag="VFA35_06340"
     CDS             1528..>2053
                     /locus_tag="VFA35_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016722555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55825.1"
                     /translation="MFDIWSAAHAGSLRFARFACLAGVIAFTGCATHYIDGSTKEVAA
                     ASFRKPAEPKPVQLLFEFQTKGALNTRATDFLKAQVTEQVKGSGLFASVDDKPVAGGG
                     LLSVTLNNVPLTDDAFTKGFVTGFTFGLVGSQVSDGYVCTVKYAAAQQNDMIVKTARH
                     AIHTAMGAKDAPPNS"
BASE COUNT          316 a          720 c          696 g          321 t
ORIGIN      
        1 agtacgtctg ccggccgcgc gccgactcgt cgcggatgcg cgcgatcacc tcgtcgcggc
       61 gcgcgtcgac gaagagcttg gtgacgacag gcgtgcggcc cggtggcagc tcgtcgatcg
      121 tgctgacgtc gagatcggcg aacagcgtca tcgcaagcgt gcgcgggatc ggcgtcgccg
      181 acatcatgag cagatgcggc tcgagcgcgc cctggtcgtg cagcttggcc cgcagtgcca
      241 accgctgagc gacgccgaag cggtgctgct cgtcgacgat cgcgagaccc agcctcgcga
      301 actgcacctg ctcctggatc accgcgtgcg tgccgacgac cagcatcgcc tcacccgacg
      361 cgacctgctc cagcatcttc gaccgcgcct tgcccttgcg gctgccggtc agccacgcga
      421 ccgtgatgcc cagcggttcg agccagccga ccagcttgcg caggtgctgc tcggcgagga
      481 tctcggtcgg cgccatcagc gcgcactgcc agccggcgtc gatcgcgatc gcggcagcga
      541 gcgcggcgac cacggtcttg ccggaaccga catcgccttg cagcaggcgg tgcatcggga
      601 cctcgcgttg caggtcggcc gcgatctcgt cgaccacgcg gcgctgcgcg gcggtgagcc
      661 tgaacggcaa ggccgcgagc agttgctcct gcagcccgcc ggcgcgcacg ccgaagcgcg
      721 gcgcgcgctg cagctcgcgc tcgcgcttgg cctgcagctg cgagagctgc tgcgccagca
      781 gctcctcgaa cttgagccgc tgccacgccg ggtggctgcg gtcttcgagc gctttgacgc
      841 cgacgttcgg cgccgggtgg tgcaggaaca gcagcgcgtc gcgcaggccc atcatgccgg
      901 tcggcaccgc gtcgggcggc agcagttccg cgagatcggt ccgcgcgagg ttggcctcga
      961 tcgccttgcg cagataggcc tgcggcagct gcgcgctggt cgggtagacc ggcgtcagcg
     1021 cggtcgcgag cggcgcgccc tcggccacgg ccttgaacgt cgggtgcacc atctccaacc
     1081 cgaagaagcc gccgcgcgcc tcgccgcgca cccgcacgcg tgtgccgacc gcgagcgcct
     1141 tctggtgcga cgggtagaag tgcaggaagc gcagcaccag gtcgtcgctg ccgtcgtgaa
     1201 tccgcacgac gagctggcgc cgaacgcgga tctgcacctc gcagtcgcgc accatgccct
     1261 cgacctggcc gacgctgccg tcgcgcaggc tcgcgatcgg cagcagccgg gtctcgtctt
     1321 cgtagcgcat cggcaggtgc agcgcgaagt cgatcgggcg caccaggccg agcttctcca
     1381 gcgcgcgctg cgggccgcat ttgtcgactt cttgccggtc tttgggggat ttcatcggca
     1441 tcgattcatt ctgcctgcag gggcgccgct acgatcgccg ccgcgcgggg aggacatcgc
     1501 gcaaaacgac aaccaccgga gatgctcatg ttcgacattt ggtcagcggc ccacgccgga
     1561 tcgctacgct ttgctcgctt cgcttgtctt gccggcgtca tcgccttcac gggttgcgca
     1621 acgcactaca tcgatggcag taccaaggaa gtggcagctg cctcgttccg caagcctgcc
     1681 gaaccgaagc cggttcaatt gctgttcgag ttccagacca agggcgcgct gaacacccgc
     1741 gcgaccgact tcctgaaggc ccaggtcacc gaacaggtca agggcagtgg cctcttcgcc
     1801 agtgtcgacg acaagccggt cgccggcggc ggcctgctca gcgtgacgct gaacaacgtc
     1861 ccgctgactg acgacgcgtt cacgaaaggc ttcgttaccg ggttcacgtt tggtctggtc
     1921 gggtcgcagg tgtcggacgg ctatgtctgc acggtcaagt acgccgcggc gcagcagaac
     1981 gacatgatcg tgaagaccgc ccggcacgcg atccacaccg cgatgggcgc caaggacgcg
     2041 ccgccgaaca gcg
//
LOCUS       DASRDG010000384         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_630404, whole genome shotgun sequence.
ACCESSION   DASRDG010000384 DASRDG010000000
VERSION     DASRDG010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630404"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /gene="tnpB"
                     /locus_tag="VFA35_06345"
     CDS             <1..332
                     /gene="tnpB"
                     /locus_tag="VFA35_06345"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HZT55826.1"
                     /translation="LKVYLHREPIDFRLNINGLALLVDKALGLDPFAPCAYVFSNRRR
                     NRVKILGWDRNGFWLLLKRLEQDRFIWPHAEVVPMLTVEQLHWLLEGIDIGVVQRHPQ
                     RLYERVA"
     gene            445..2046
                     /locus_tag="VFA35_06350"
     CDS             445..2046
                     /locus_tag="VFA35_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZT55827.1"
                     /translation="MPTAEEIAALRQALTDAIALGEQAARRSVELAGELRVVRTERDL
                     LKEQLNRFKRQLFDAKSEASAAHQKDMFFNEAEVEGAQAQPAAAETEDEKIDVPAHQR
                     TKRGRKPLDPALPRDVRRHELPEDERVCPHDGARLSEIGVEVSEQMDVVPQQVRVIRH
                     ERVKYACPCCDGGLRLAAKPPQVIPKGLLSEAALAWVITSKYLDGLPLYRQAVLLGRF
                     GGTELSRNTLAASVVRVGQAVQPVVNLLRDALLDAFIVHGDETEVQVLKEPGRKAQAK
                     SYMWVQMTEASGTQGTGPPIRLFGYSPSRSTHSARTLYEGMRPGGVLMSDGYEPYAAI
                     AEQHRLVHLGCWAHCHRYFNDALQALPKDKRGPEQLPARFIELIGRLYKLEADARRDN
                     LDAQARQQQRKLDSVPVLEQIEALALQHLHGVLPGSLLGKALHYLTAQWPKLVRYVDD
                     GRYPIDNNACENAIRPFVVGRRNWLFADTVGGANASANLYSLLQTCLANGIDGYRYLT
                     ALLVELPKATTVEDYEALLPWRLTQ"
     gene            complement(2081..>2273)
                     /locus_tag="VFA35_06355"
     CDS             complement(2081..>2273)
                     /locus_tag="VFA35_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZT55828.1"
                     /translation="AALALQIPQAVLDMHAHWLPLRLAVYQREVAKSMQPKVGRNEPC
                     PCGSGKKFKRCCGAAGNMH"
BASE COUNT          392 a          759 c          775 g          347 t
ORIGIN      
        1 ccctgaaggt gtacctgcac cgcgagccga tcgacttccg actcaacatc aacggcctgg
       61 cgctgctggt ggacaaggcg ctggggctgg atccgttcgc gccgtgcgcg tacgtgttca
      121 gcaaccggcg gcgcaaccgc gtgaagatcc tcggctggga ccgcaacggg ttctggctgc
      181 tgctcaaacg actggagcag gaccgcttca tctggccgca cgccgaggtg gtgccgatgc
      241 tcacggtgga gcaactgcac tggctgctcg agggcatcga catcggcgtc gtgcagcgcc
      301 atccccagcg gctgtacgag cgcgtcgcct gagccgcacc actgtacggg ttggttgcgc
      361 gcggtccgca ttgttacggg ccgatgccaa gattgcgggt acgccggcac gcgcgggtga
      421 tgacaatgcg cgccgatggt tcacatgccc accgccgaag agatcgccgc actgcgccaa
      481 gcgctgaccg atgccattgc gcttggcgag caggccgctc ggcgtagcgt tgagctcgcg
      541 ggtgagctgc gcgtggtgcg caccgaacgc gacctgctca aggagcagct caaccgcttc
      601 aagcgccagc tcttcgacgc caagagcgag gccagcgccg cgcaccagaa ggacatgttc
      661 ttcaacgaag cggaggtcga aggtgcacag gcccagcctg cggccgccga gaccgaggac
      721 gagaagatcg atgtgccggc gcaccagcgc accaagcgcg gccgcaagcc gctggatccg
      781 gccctgccgc gcgacgtgcg gcgccacgaa ttgcccgagg acgagcgcgt gtgcccgcac
      841 gacggcgcgc gcttgagcga gatcggcgtg gaggtcagcg agcaaatgga cgtcgtgccg
      901 cagcaggtgc gcgtgatccg tcacgagcgg gtcaagtacg cgtgcccctg ctgcgacggc
      961 ggtctgcgcc tggcggccaa gccaccgcag gtgatcccca agggcttgct cagcgaagcg
     1021 gcgctggcct gggtgatcac gtcgaagtac ctggatgggc tgccgctgta ccggcaggcc
     1081 gtgctgctgg gccgcttcgg cggcacggag ctgtcgcgca acacgctggc cgccagcgtc
     1141 gtgcgggtgg gccaggcggt gcagccggtg gtcaacctgc tgcgcgacgc gctgctggac
     1201 gcgttcatcg tccacggcga cgagaccgag gtgcaggtgc tcaaggagcc gggacgaaag
     1261 gcccaggcca agagctacat gtgggtgcag atgaccgaag ccagcggcac ccaaggcacc
     1321 gggccgccga tccgcctgtt cggctactcg cccagccgca gcacgcactc ggccaggaca
     1381 ctgtacgagg gcatgcggcc cggcggcgtg ctgatgagcg acggctacga gccatatgcg
     1441 gcgatcgccg agcagcatcg gctcgtgcat ctgggctgct gggcgcattg ccaccggtac
     1501 ttcaacgatg cgctgcaggc gttgcccaag gacaagcgcg ggccggagca gttgccggcg
     1561 cgcttcatcg agctcatcgg ccggctgtac aagctcgagg ccgacgcccg ccgggacaac
     1621 ctggacgccc aggcgcgcca gcaacagcga aagctggaca gcgtgccggt gctcgagcag
     1681 atcgaggccc tggcgctgca gcacctgcac ggcgtgttgc cgggcagtct gttgggcaag
     1741 gcattgcact acctcaccgc gcagtggccc aagctcgtgc gctacgtcga tgacggccgc
     1801 tacccgatcg acaacaacgc ctgcgaaaac gcgatccgcc cgtttgtcgt cggacgccgc
     1861 aattggttgt tcgccgacac ggtgggcggc gccaacgcca gcgccaacct gtactcgctt
     1921 ctgcagacct gcctggccaa cgggatcgac ggctaccgct acctcacggc gctgctcgtc
     1981 gagttgccca aggccacaac cgtcgaggac tacgaggcgc tgctgccttg gcgactgacg
     2041 cagtgaattg gccttgaggc cgcgcagtcc acgatcttcc tcaatgcatg ttccccgccg
     2101 ctccgcagca tcgcttgaac ttcttgccgc tgccgcaggg gcaaggctca ttgcgcccca
     2161 ccttgggttg catcgacttc gccacctcac gctgatagac agcgagtctg agcggtagcc
     2221 agtgcgcatg catgtctagg acagcttgcg ggatttggag tgccagcgcc gcc
//
LOCUS       DASRDG010000385         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1441703, whole genome shotgun sequence.
ACCESSION   DASRDG010000385 DASRDG010000000
VERSION     DASRDG010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441703"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VFA35_06360"
     CDS             <1..1115
                     /locus_tag="VFA35_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZT55829.1"
                     /translation="LAAKRPYIYTGGGVILGNATQELRTLVDMLGYPCTNTLMGLGAY
                     PASDPKFLGMLGMHGTYEANMTMQNCDVLLAVGARFDDRVIGNPKHFASVERKIIHID
                     IDPSSISKRVRVDIPIVGDVRDVLQELIVQIRDAQVKPDVTVISAWWKQINEWRKKDC
                     LAFKNSTEVIKPQHVVHRLWELTKDSDAYITSDVGQHQMWAAQYYRFDRPRRWINSGG
                     LGTMGVGLPYAMGIKLAKPEADVFCITGEGSIQMCIQELSTCLQYKTPVKIISLNNRY
                     LGMVRQWQQLDYGGRYSHSYMDALPDFVKLAEAYGHVGLKVEKPSDVDGALREAIRLK
                     DRTVFLDIRTDPTENVWPMVQAGKGISEMLLGSEDL"
     gene            1279..1770
                     /gene="ilvN"
                     /locus_tag="VFA35_06365"
     CDS             1279..1770
                     /gene="ilvN"
                     /locus_tag="VFA35_06365"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZT55830.1"
                     /translation="MKHIIALLLENEPGALSRVVALFSARGYNIESLTVATTEDASLS
                     RMTIVTKGSDEVIEQITKHLNRLIEVVKVVDLTEGAFTERELMLIKVRAVGKEREEMK
                     RMADIFRGRIIDVTDKSYTIELTGDTGKLDAFLEAIDRAAILETVRTGTSGIGRGERI
                     LRV"
     gene            1820..2836
                     /gene="ilvC"
                     /locus_tag="VFA35_06370"
     CDS             1820..2836
                     /gene="ilvC"
                     /locus_tag="VFA35_06370"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZT55831.1"
                     /translation="MKVYYDKDADLSLIKGKNVTIIGYGSQGHAHAQNLSDSGVKVTV
                     GVRKGGPSWAKAEKAGLKVAEVADAVRQADVVMMLLPDEQIGAVYKSEVEPNIKQGAS
                     LAFAHGFNVHYGQVVPRADLDVWMVAPKAPGHTVRSTYTQGGGVPHLIAVHADKTGKA
                     RDLALSYAAANGGGKAGVIETNFREETETDLFGEQAVLCGGAVELIKAGFETLTEAGY
                     APEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEYGEYVTGPKIVTDETKKAMKQ
                     ALKDIQNGEYAKSFIVENRAGAPTLMSRRRITAEHPIEVVGEQLRAMMPWIKKNRLVD
                     QSKN"
     gene            2859..>3214
                     /locus_tag="VFA35_06375"
     CDS             2859..>3214
                     /locus_tag="VFA35_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZT55832.1"
                     /translation="MNDTPPLPDDEPALGAETRPRRKGLYVLPNAITLAALFSGFYAI
                     VMAINGRFELSCIAIFCAAVLDSLDGRVARMTNSQSAFSEQMDSLSDMVSFGAAPALI
                     VYIWALKDLGKAGWIPA"
BASE COUNT          633 a         1037 c         1054 g          490 t
ORIGIN      
        1 tgctggcagc caagcggccc tacatctaca ccggcggcgg cgtgatcctg ggcaacgcca
       61 cccaggaact gcgcacgctg gtcgacatgc tgggttaccc ctgcaccaac acgctgatgg
      121 gcctgggcgc ctatccggcg agcgacccga agttcctcgg catgctcggc atgcacggca
      181 cttacgaggc caacatgacg atgcagaact gcgacgttct gctggccgtc ggcgcgcgct
      241 tcgacgaccg cgtgatcggc aacccgaagc acttcgcgtc ggtcgagcgc aagatcatcc
      301 acatcgacat cgacccgtcg tcgatctcga agcgggtgcg cgtcgacatc ccgatcgtcg
      361 gcgatgtgcg ggacgtgctg caggagctga tcgtgcagat ccgcgacgcg caggtgaagc
      421 ccgacgtgac cgtgatctcg gcctggtgga agcagatcaa cgagtggcgc aagaaggact
      481 gcctcgcttt caagaacagc accgaggtca tcaagccgca gcacgtggtg cacaggctct
      541 gggagctgac gaaagacagc gacgcctaca tcacgtcgga tgtcggccag caccagatgt
      601 gggctgcgca gtactaccgc ttcgaccgcc cgcgccgctg gatcaactcc ggcggcctgg
      661 gcacgatggg cgtgggcctg ccgtatgcga tgggcatcaa gctcgcgaaa cccgaggccg
      721 acgtgttctg catcaccggc gaaggctcga tccagatgtg catccaggag ctctcgacct
      781 gcctgcagta caagacgccg gtcaagatca tctcgttgaa caaccgctac ctcggcatgg
      841 tgcggcagtg gcagcagctc gactacggcg gccgctactc gcacagctac atggacgcgc
      901 tgcccgattt cgtcaagctc gccgaggcct acggccacgt cggcctgaag gtcgagaagc
      961 cgtcggacgt cgacggcgcg ctgcgcgagg cgatccgcct gaaggaccgc accgtgttcc
     1021 tcgatatccg caccgacccg accgagaacg tctggccgat ggtgcaggcg ggcaagggca
     1081 tctccgaaat gctgctgggc tccgaagacc tgtgacgcgc tgaacgccgc ggcggggcag
     1141 ggcgttaccg cgctcggccc gggcctcggc gggatgcgtc gatcgacttc ggccaattca
     1201 ctgacctctg aacgaaagca gcgtggccaa gggccgggcg gttaccgccg ccggtccgaa
     1261 gagcgcgctg acatccacat gaagcacatc attgcactgc tgctcgagaa cgagccgggc
     1321 gcactctcgc gcgtcgtcgc gctgttctcg gcgcgtggtt acaacatcga gagcctgacg
     1381 gtcgcgacga ccgaagacgc ctcgctctcg cgcatgacga tcgtcaccaa gggctccgac
     1441 gaagtcatcg agcagatcac caagcacctg aaccggctga tcgaggtcgt caaggtcgtc
     1501 gacctgaccg aaggtgcgtt caccgagcgc gaactgatgc tgatcaaggt gcgcgccgtc
     1561 ggcaaggagc gcgaggagat gaagcgcatg gccgacatct tccgcggccg catcatcgac
     1621 gtgaccgaca agagctacac gatcgaactg accggcgaca ccggcaagct cgacgccttt
     1681 ctggaggcga tcgaccgcgc cgccattctc gaaaccgtgc gcaccggcac cagcgggatc
     1741 ggtcgcggcg agagaatcct tcgggtctag gacagcgggc tctgcggagc ccttcaactt
     1801 ttttctttgg agaacgaaca tgaaggtcta ctacgacaag gacgccgatc tgagcctgat
     1861 caagggcaag aacgtcacga tcatcggcta tggctcgcag ggccacgccc acgcgcagaa
     1921 cctgagcgac agcggcgtca aggtcacggt cggcgtgcgc aagggcggcc cgtcgtgggc
     1981 gaaggccgag aaggccggcc tgaaggttgc cgaggtggcc gacgccgtcc ggcaggccga
     2041 cgtggtcatg atgctgctgc ccgacgagca gatcggcgcg gtctacaaga gcgaggtcga
     2101 gccgaacatc aagcagggcg cctcgcttgc gttcgcgcac ggcttcaacg tgcactacgg
     2161 ccaggtcgtg ccgcgcgccg acctcgacgt ctggatggtc gcgcccaagg ccccgggcca
     2221 caccgtgcgc tcgacctaca cccagggcgg cggcgtgccg cacctgatcg cggtacatgc
     2281 cgacaagacc ggcaaggcaa gagatctggc gctgagctac gcggccgcca acggcggcgg
     2341 caaggccggc gtcatcgaga ccaacttccg cgaagagacc gagaccgacc tgttcggcga
     2401 gcaggctgtg ctgtgcggcg gtgcggtcga actgatcaag gccggtttcg agaccctgac
     2461 cgaagcgggc tacgcgcccg agatggcgta cttcgagtgc ctgcacgagc tcaagctgat
     2521 cgtcgacctg atctatgaag gcggcatcgc caacatgaac tactcgatct cgaacaacgc
     2581 cgagtacggc gagtatgtga ccgggccgaa gatcgtcacc gacgagacca agaaggcgat
     2641 gaagcaggcg ctcaaggaca tccagaacgg cgagtacgcg aagagcttca tcgtcgagaa
     2701 ccgcgccggc gcgccgacgc tgatgagccg ccgtcgcatt accgccgagc acccgatcga
     2761 agtggttggc gagcagctgc gcgcgatgat gccgtggatc aagaagaacc gcctcgtcga
     2821 ccagtcgaag aactgacccg caaggtatcc tcgcgcggat gaacgacacg cctcccttgc
     2881 ccgacgacga gcctgcgctc ggggccgaga cccggccgcg acgcaagggc ttgtatgtgc
     2941 tgcccaacgc gatcacgctg gcggccctgt tctcgggctt ctacgcgatc gtgatggcga
     3001 tcaacgggcg tttcgagctg tcgtgcatcg cgatcttctg cgccgcggtg ctcgacagcc
     3061 tggacggccg cgtcgcgcgc atgacgaact cgcagagcgc gttcagcgag cagatggaca
     3121 gcctgtccga catggtgagt ttcggcgccg cgccggcgct gatcgtctac atctgggcgc
     3181 tgaaggatct gggcaaggcg ggctggattc cggc
//
LOCUS       DASRDG010000386         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_514058, whole genome shotgun sequence.
ACCESSION   DASRDG010000386 DASRDG010000000
VERSION     DASRDG010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514058"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFA35_06380"
     CDS             complement(<1..269)
                     /locus_tag="VFA35_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55833.1"
                     /translation="MRRRLLALATALATSAGLPAAHAQDRVLDDFENPAAWQLGATDD
                     VRAALRSADGAHGKALCIDFDFGRVTGYVSARRELPIDYPAHYEF"
     gene            complement(266..1087)
                     /locus_tag="VFA35_06385"
     CDS             complement(266..1087)
                     /locus_tag="VFA35_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZT55834.1"
                     /translation="MNARWAVWVVNGLLAGLAFVSLFPLLWMLSVSFMPAGASSTLPT
                     PIFPSTVTLDNYRQLFGSIGMGRYFMNSLALALLSSAISVSFNVMAGYAFAKLRFRGR
                     DAIFKTLLGALVIPGQVAMLPLFLMLKPLGLINSYAGAVVPMMASVFGIFLVRQFARG
                     IPDDLLEAARIDGASEWRIFLTIVVPLLAPVLATLAIFSFLGSWNDFMWPLIVLTDDS
                     LHTLPVALAGLSREHVQDNELMMAGSVVTILPVLLVFLALQRHYMDGLMAGSVKG"
     gene            complement(1084..1971)
                     /locus_tag="VFA35_06390"
     CDS             complement(1084..1971)
                     /locus_tag="VFA35_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZT55835.1"
                     /translation="MRKTQLVGWALAAPALVVIAVFFALPVAAGLLLSLTDFDLYALA
                     DLSTLRFVGLQNYARLLQTPLFWQALGNTFYFVVVGVPVSIGLSLGAALLLNARVAKW
                     QPLFRTALFAPVVTTVVAVAVIWRYLLHTRYGLINQGLESIGVGPVDWLGNPHWAMPA
                     IILFAAWKNFGYNMVILLAALQAVPRELYESARVDGARPLRQLVDLTLPMLTPTLVMV
                     GIMTITGYFQLFAEPYVMTQGGPLRSTVSVLYFMYEEGFRWWNLGNASAVAFALFVVI
                     FAVSWVLLRFDRDPTGATA"
     gene            complement(1968..>3034)
                     /locus_tag="VFA35_06395"
     CDS             complement(1968..>3034)
                     /locus_tag="VFA35_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZT55836.1"
                     /translation="TAYAGGALPDLCQLGNTWLPEFTALDALEPLDARLAGSGVPRDD
                     YFAGILDTNVMPTAQGPRLFGLPWYVDTRLLFYRSDLLRAAGFAQAPRSWAEWLDAMR
                     AIRQRGGRNGFGALLPLNEPDPLFALALQQGALLADDDTRGAFSKPAFRRALDFYAGI
                     FHEGLAPVMTDAQISNVWDEFARGMFAFYVSGPWNIGEFRRRLPTALQGRWATAPLPG
                     PDGPGVSTAGGSSLVLFKSSRRKEAAWRLIEFLSETDTMQRFHALTGDLPPRRSAWRA
                     PALALDAPSQAFAQQLERVRAAPKIPEWERIFQEMQQTAQRVVQRVQGAEAATAQLDA
                     WVDALLEKRRWLRARAAAAR"
BASE COUNT          520 a         1046 c         1079 g          389 t
ORIGIN      
        1 atgaattcgt agtgcgccgg gtagtcgatc ggcaattcgc gccgcgcgct gacgtagccg
       61 gtgacgcggc cgaaatcgaa gtcgatgcac agcgccttgc cgtgcgcgcc gtcggcggag
      121 cgcagcgcgg cgcgcacatc gtcggtcgcg ccgagctgcc aggccgcggg gttctcgaag
      181 tcgtcgagca cgcggtcctg cgcgtgcgcg gccggcaggc ccgctgacgt tgcaagcgcg
      241 gtggccagcg cgagcagccg ccgcctcatc ccttcacgct gccggccatc agcccgtcca
      301 tgtagtgccg ctgcagcgcg aggaacacga gcagcaccgg caggatcgtc acgaccgagc
      361 cggccatcat cagctcgttg tcctgcacat gctcgcgcga caggccggcg agcgcgaccg
      421 gcagcgtgtg cagcgagtcg tcggtcagca cgatcagcgg ccacatgaag tcgttccagc
      481 tgccgaggaa cgagaagatc gcgagcgtcg cgagcaccgg cgcgagcagc ggcacgacga
      541 tggtcaggaa gatgcgccac tcgctcgcgc cgtcgatgcg cgcggcttcg agcaggtcgt
      601 cggggatgcc gcgcgcgaac tggcgcacca ggaagatgcc gaacacgctc gccatcatcg
      661 gcaccacggc gccggcgtag ctgttgatca gcccgagcgg cttgagcatc aggaacagcg
      721 gcagcatcgc gacctgcccg gggatcacca gcgcgccgag cagcgtcttg aagatcgcgt
      781 cccgcccgcg aaagcgcagc ttcgcgaacg cgtagccggc catcacgttg aagctgaccg
      841 agatcgcgct cgagagcagc gcgagcgcga ggctgttcat gaagtagcgg cccatgccga
      901 tgctgccgaa gagctggcgg tagttgtcca gcgtgaccgt cgacgggaag atcggcgtcg
      961 gcagcgtgct cgacgcgccg gccggcatga acgagaccga cagcatccac agcagcggga
     1021 acaggctgac gaaggcgagc cccgccaaca agccattgac gacccacacc gcccaacgcg
     1081 cattcatgcg gtcgctcccg tcgggtcgcg gtcgaagcgc aacagcaccc agctcaccgc
     1141 gaagatcacg acgaacagcg caaacgcgac cgcgctcgca ttgccgaggt tccaccagcg
     1201 aaagccctct tcgtacatga agtacagaac gctgaccgtg ctgcgcagcg gcccgccctg
     1261 cgtcatcaca tagggttcgg cgaagagctg gaagtagccg gtgatcgtca tgatgccgac
     1321 catcacgagc gtgggtgtca gcatcggcaa ggtcaggtcg acgagctggc gcagcggccg
     1381 ggcgccgtcg acgcgcgccg attcgtagag ctcgcgcggc accgcctgca gcgcggccag
     1441 caggatgacc atgttgtagc cgaagttctt ccaggccgcg aacaggatga tcgccggcat
     1501 cgcccagtgc gggttgccga gccagtcgac cggcccgacg ccgatcgatt ccaggccctg
     1561 gttgatcagc ccgtagcgcg tgtgcagcag gtagcgccag atcaccgcga ccgcgacgac
     1621 cgtggtcacg accggcgcga acagcgcggt gcggaacagc ggctgccact tcgcgacgcg
     1681 cgcgttcagc agcagcgccg cgccgagcga caggccgatc gacaccggca cgccgaccac
     1741 gacgaaatag aacgtattgc ccagcgcctg ccagaacagc ggcgtctgca gcagccgcgc
     1801 gtagttctgc aggccgacga aacgcagcgt cgacaggtcg gcgagcgcgt agaggtcgaa
     1861 gtcggtcagg ctcagcagca ggccggccgc gaccggcagc gcgaagaaca ccgcgatcac
     1921 gaccagcgcc ggcgccgcca gcgcccagcc gacgagttgg gtttttctca ccgcgcggcc
     1981 gccgcgcgcg cgcgcagcca gcgccgcttc tcgagcagcg cgtcgaccca tgcgtcgagc
     2041 tgcgcggtgg cggcctcggc gccttgcacg cgctgcacga cgcgctgcgc ggtctgctgc
     2101 atctcctgga agatgcgctc ccattcgggg atcttcggcg ccgcgcgcac gcgctcgagc
     2161 tgctgcgcga aggcctgtga cggcgcgtcg agggcgagcg ccggcgcgcg ccacgcgctg
     2221 cggcgcggcg gcaggtcgcc ggtcagcgcg tggaagcgct gcatcgtgtc ggtctcgctg
     2281 aggaactcga tcagccgcca ggcggcctcc ttgcgccggc tgctcttgaa cagcaccagg
     2341 ctcgagccgc ccgcggtcga cacgccgggg ccgtcaggcc cgggcagcgg cgcggtcgcc
     2401 cagcggccct gcagcgccgt aggcaggcgg cgccggaact cgccgatgtt ccacgggccg
     2461 ctgacgtaga aggcgaacat gccgcgcgcg aattcgtccc agacgttcga gatctgcgcg
     2521 tcggtcatca ccggcgcgag gccttcgtga aagatgccgg cgtagaagtc gagcgcgcgc
     2581 cggaacgccg gcttcgagaa cgcgccgcgc gtgtcgtcgt cggcgagcag cgcgccttgc
     2641 tgcagtgcga gcgcgaacag cgggtcgggt tcgttcagcg gcagcagcgc gccgaagccg
     2701 ttgcggccgc cgcgctgccg gatcgcgcgc atcgcgtcca gccactcggc ccagctgcgc
     2761 ggcgcctgcg caaagccggc ggcgcgcagc agatcgctgc ggtagaacag cagccgggtg
     2821 tcgacatacc acggcaggcc gaacaggcgc gggccttgcg cggtcggcat cacgttggtg
     2881 tcgaggatgc cggcgaagta gtcgtcgcgc ggcacgccgg agccggcgag gcgtgcatcg
     2941 agcggctcga gcgcgtcgag cgcggtgaat tcgggcagcc aggtgttgcc gagctggcac
     3001 agatcgggca gcgcgccgcc ggcgtaggcg gtca
//
LOCUS       DASRDG010000387         4486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_766951, whole genome shotgun sequence.
ACCESSION   DASRDG010000387 DASRDG010000000
VERSION     DASRDG010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766951"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1323)
                     /locus_tag="VFA35_06400"
     CDS             complement(1..1323)
                     /locus_tag="VFA35_06400"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HZT55837.1"
                     /translation="MRTLISRRTFCAGTLLPATAALLLPGCGGGGSDPTQSAAFSVRG
                     HAFATAAPALWADIAMRAAAVLPPPGLPPFITARAYAMAFLAAHDALNAIVPVYATYL
                     GGPKAPGANPDAAVAAAVHDVLVHEFPFATALLDSAYASALAALAGDGSVSKGLEIGR
                     SCAAAMLAARANDGLADVEGPFTEGTQPGEYRFTPPFDFAATVRWGDVMKPFSIASAA
                     AYRVGKPYVVTDDAYTADYNEVKALGAAVGSSRTADQSELGKFWLENTNDSWMKIALQ
                     LAASRQMDGWALMRTLALIQVAQTDAYTACIESKYFYHFWRPITAIRLGDSDGNPATV
                     GDPGWGSFDPVCPPIPDYPSGHSASGGAGAVVLAQAFGSDALGFTHQSVSLPGPTRSF
                     ASFSQAQDEIALSRICVGYHFRLATQAGVAQGRAVATQVMATQLPALG"
     gene            complement(1368..2660)
                     /locus_tag="VFA35_06405"
     CDS             complement(1368..2660)
                     /locus_tag="VFA35_06405"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HZT55838.1"
                     /translation="MRVITVSVPAVIALACATAFIGACGGGDDDSVAPVTVTVTGPNA
                     VSQWNEIAATTINLPAAATGTPEEQRPTYAVDLATVHLAIYDAVMAIVGTHKPYAYAP
                     ASLSAGASQEAAVATAAYRVLLALFPARSASYQSAYDSAIASIAAGAAKDQGIAVGAA
                     AADVVVALRANDGRAVVLAPFVPGTAPGQFRGVNPVNRYAPYIKPFAVTSLAQFRAPG
                     PPALTSASYAADVNETMALGSATSTTRTAAQTEIARFHTEAPPVFWTRNLRNFAMTNR
                     SLAEQARLLALLWVVQADATNTCFESKYNFLFWRPTSAITLADTDGNPATTVDAAWTP
                     VVPTPNHPEYPAAHGCVSSSMMSALAGYYGTPNVTFDINSTVTGSTHSFTTTTAFVEE
                     IKLARIAGGMHFRTSTIDGAALGKNVAEWVLAHNFQPR"
     gene            2873..>4486
                     /locus_tag="VFA35_06410"
     CDS             2873..>4486
                     /locus_tag="VFA35_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55839.1"
                     /translation="MAVKRAAMPAKLSPPRVRQALPRERLFAWLDARRNHPGLWLAGR
                     PGAGKTMLAVGWLEARALPFLWYRLDTDDNDVGRFFDMLGDAAKSLAPRLRPPPFAAE
                     HVAQWPAFARSWFRRVFSALPGPAVLVFDNVEQAALPALPQLLAIALDEAPTGITLLM
                     TSRHEPPPALAGAVLAGTLAVLAPEELRFRPEEARHYARALGLDEEALARAAVRVDGW
                     AAGVRLISHSGVAAEAGASPPLLFDYFAGLLHASLDRAAQRLLLIGALLPWMPGALLA
                     ELAGAPDAVEHLERLCAGNLFTERTGQVPAVYRLHPLFRAFLLERGRSVLAPGERSAL
                     LQHAARAFLALGHTDIAIDLQLDAGDLASAADLWLAVLETKLAHGQLDQLDAWSARFG
                     DACIPQRPWLQYGRARICFLREHPTARTHYEDAFATFAALGDRAGQQLCAAGLLEWHY
                     NTDSFIGHERWCESLAQPLPDSVRSEVHALRLLNGQLLACFFRGDFRRDAPGWTNRVL
                     ARLTLGEAENDKLCLAITLLGCLERDKRWD"
BASE COUNT          627 a         1599 c         1595 g          665 t
ORIGIN      
        1 tcagcccagc gccggcagct gcgtcgccat cacctgcgtc gcgaccgcac gcccttgcgc
       61 cacgccggcc tgcgtcgcga gccggaagtg atagcccacg cagatgcgcg acagcgcgat
      121 ctcgtcctgc gcctgcgaaa acgaggcgaa gctgcgggtc gggccgggca ggctgacgct
      181 ctggtgcgtg aagcccagcg cgtcgctgcc gaaggcctgc gccagcacca cggcgccggc
      241 gccgcccgag gccgagtggc ccgacgggta gtcggggatc ggcgggcaca ccgggtcgaa
      301 cgagccccaa ccgggatcgc cgacggtcgc cgggttgccg tcgctgtcgc caagccggat
      361 cgctgtgatc gggcgccaga agtggtagaa gtatttcgac tcgatgcacg cggtgtacgc
      421 gtcggtctgc gccacctgga tcagcgcgag cgtgcgcatc agcgcccagc catccatctg
      481 ccgcgacgct gcgagttgca gcgcgatctt catccacgag tcgttggtgt tttcgagcca
      541 gaacttgccg agttccgact ggtcggcggt gcgcgacgaa ccgaccgcgg cgccgagcgc
      601 cttgacctcg ttgtagtcgg ccgtgtatgc gtcgtcggtg acgacgtagg gcttgcccac
      661 ccgatacgcc gccgcgctgg cgatcgagaa cggcttcatc acgtcgcccc agcgcaccgt
      721 ggcggcgaag tcgaacggcg gcgtgaagcg gtactcgccg ggctgcgtgc cttcggtgaa
      781 cgggccttcg acatcggcca ggccatcgtt ggcgcgcgcg gccagcatcg cggcggcgca
      841 gctgcggccg atctcgaggc ctttcgacac cgagccgtca ccggccagcg ccgccagcgc
      901 gctcgcatac gcgctgtcga gcagcgccgt cgcaaagggg aactcgtgca cgagcacgtc
      961 atgcaccgcg gcggcgaccg cggcatcggg gttcgcgccg ggcgccttcg ggccgcccag
     1021 gtaggtggca tacaccggca cgatcgcgtt gagcgcgtcg tgcgcggcga gaaacgccat
     1081 cgcataggcg cgcgcggtga tgaacggcgg caggccgggc ggcggcagca ccgcggcggc
     1141 acgcattgcg atgtccgccc acagcgccgg cgcggcggtg gcgaaggcgt ggccgcgcac
     1201 cgagaacgcg gccgattgcg tcggatcgct cccgccgccg ccgcagcccg gcagtaacag
     1261 ggcggcggtg gccggcagca aggtgccggc gcagaacgtg cgtcttgaaa tcagggttct
     1321 catgatgact ctcctgtgcg tgatcgatcg agcgatgcgc gcactgctca gcgcggctgg
     1381 aagttgtgcg ccaacaccca ctcggcaacg ttcttgccga gcgccgcgcc gtcgatcgtc
     1441 gaggtgcgga agtgcatgcc cccggcgatg cgcgccagct tgatctcttc gacgaaagcg
     1501 gtggtcgtcg tgaagctgtg ggtgctgccg gtgaccgtgc tgttgatgtc gaaggtcacg
     1561 ttgggcgtgc cgtagtagcc ggcgagcgcg ctcatcatcg agctgctgac gcagccgtgc
     1621 gcggccgggt actcggggtg attcggcgtc ggcaccacgg gcgtccaggc cgcgtcgacc
     1681 gtggtcgccg ggttgccgtc cgtgtcggcc agcgtgatcg cgctggtcgg gcgccagaac
     1741 aggaagttgt acttcgactc gaagcaggtg ttcgtggcat cggcctgcac gacccacagc
     1801 agcgccagca gccgcgcctg ttcggcgagg ctgcggttcg tcatcgcgaa gttgcgcagg
     1861 ttgcgggtcc agaagaccgg tggcgcctcg gtgtggaagc gcgcgatctc ggtttgggcc
     1921 gcggtgcgcg tggtgctggt ggccgaaccg agcgccatcg tctcgttcac gtcggcggcg
     1981 tagctcgcgc tggtcagcgc gggcgggccg ggggcgcgga attgcgcgag cgacgtcacc
     2041 gcgaacggct tgatgtacgg cgcgtagcgg ttgacggggt tcacgccgcg gaactgcccg
     2101 ggcgccgtgc ccggcacgaa cggcgccagc accaccgcgc ggccgtcgtt ggcgcgcagc
     2161 gcgacgacca cgtcggcggc cgcggcaccc acggcgatcc cctggtcctt cgccgcaccg
     2221 gccgcaatcg acgcgatggc gctgtcgtag gcgctctggt acgacgcgct gcgcgccggg
     2281 aacagcgcca gcagcacccg gtaggcggcg gtggcgaccg cggcctcctg cgaagcgccg
     2341 gccgacaggc tggccggcgc gtaggcatac ggcttgtgcg tgccgacgat cgccatcacc
     2401 gcgtcgtaga tcgcaaggtg cacggtcgcg agatcgaccg cataggtcgg ccgctgctcc
     2461 tcgggcgtgc cggtcgcggc ggcaggcagg ttgatcgtcg tcgcggcgat ctcgttccat
     2521 tgcgagaccg cattcgggcc ggtgacggtg acggtgaccg gagccaccga atcgtcgtcg
     2581 ccgccgccgc aggcgccgat gaacgccgtg gcgcacgcca gtgcgatgac cgccggcacg
     2641 ctgacagtga tcactctcat gttcgctcct tgttgcacgg gatgtgcggg ccgcagtctg
     2701 cgcgcgtcga ccgcgtcacg cggcggattg cgacgagtcc cgcgcgtgca ggttcgaacg
     2761 cgccgtagcg ccgacgaaac cgaaccaggc gcttgcccgc cgcggcggcg atggcctagc
     2821 atcggcgcac gccgcgggca gccgcctgcg gcgtcctcca cgagcaagcg agatggccgt
     2881 caaacgtgcc gccatgccgg ccaagctgag cccgccccgg gttcgccaag cgctgccgcg
     2941 cgagcgtctg ttcgcctggc tcgatgcacg ccggaatcac ccggggttgt ggctcgccgg
     3001 ccggcccggc gcgggcaaga ccatgctggc ggtcggttgg ctcgaggcgc gggcgctgcc
     3061 ctttctgtgg taccggctcg acaccgacga caacgacgtc gggcgcttct tcgacatgct
     3121 gggtgatgcg gccaagagcc tcgcgccgcg cctgcggccg ccgccgttcg cggccgagca
     3181 tgtcgcgcaa tggccggcgt tcgcacgcag ctggttccgc cgcgtcttca gcgccctgcc
     3241 ggggcctgcg gtgctggtgt tcgacaacgt cgagcaggcc gcgctgccgg cattgccgca
     3301 actgctcgcc attgcgctcg acgaagcacc gaccggcatc accttgctga tgaccagccg
     3361 gcacgaaccg ccgcccgcgc tcgccggcgc cgtgctcgcc ggcacgctcg cggtgctcgc
     3421 gcccgaagaa ctgcgcttcc ggccggagga agcacgccac tacgcacgcg cgctcgggct
     3481 cgacgaagag gccttggcga gagccgccgt gcgcgtcgac ggctgggccg ccggcgtgcg
     3541 cctgatcagc cacagcggcg tggccgccga agccggtgcc tcgccgccgc tactcttcga
     3601 ctacttcgcg ggcctgctgc acgccagcct cgaccgcgcg gcgcaacgcc tgctgctaat
     3661 cggcgcgctg ctgccatgga tgccgggggc actgctcgcc gagctggccg gcgcacccga
     3721 cgcggtcgag cacctggagc gcttgtgcgc cggcaatctg ttcaccgaac gcaccgggca
     3781 agtcccggcc gtctaccggc tccacccgtt gttccgcgcg ttcttgctcg aacgcggccg
     3841 cagcgtgctc gcacccggcg agcgcagcgc cttgctgcag cacgccgcac gcgcttttct
     3901 cgccctcggg cacaccgaca tcgcgatcga tttgcagctc gatgccggcg acctcgccag
     3961 cgccgccgac ctctggctcg cggtgctcga aaccaaactc gcgcacggcc agctcgatca
     4021 gctcgacgcg tggtcggcgc gcttcggcga cgcgtgcatc ccccaacggc cgtggctgca
     4081 gtacggccgc gcccgcatct gcttcctgcg cgagcacccg accgcgcgca cgcactacga
     4141 agacgcgttc gcgacgttcg ccgcgctcgg cgaccgtgcg ggccagcagc tgtgcgcggc
     4201 cggactgctc gagtggcact acaacaccga cagcttcatc ggccacgaac gctggtgcga
     4261 atcgctcgcg cagccgctgc ccgacagcgt gcgcagcgaa gtgcacgcgc tgcgtctgct
     4321 caacggccag ctgctcgcct gcttctttcg cggcgacttc cgccgggacg cgcccggctg
     4381 gaccaatcgc gtgctcgcgc ggctgaccct cggcgaagcc gagaacgaca agctctgcct
     4441 cgcgatcacg ctgctcggtt gcctcgaacg cgacaagcgc tgggac
//
LOCUS       DASRDG010000388         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_514166, whole genome shotgun sequence.
ACCESSION   DASRDG010000388 DASRDG010000000
VERSION     DASRDG010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514166"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1905)
                     /locus_tag="VFA35_06415"
     CDS             complement(<1..1905)
                     /locus_tag="VFA35_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55840.1"
                     /translation="MLLALISVPLLLLRGRGSIHRLVALAPRSARLVEPRLTGGFPWA
                     AYAGVHRAAGESKDAAQLKLGGAAGEIVEGAARNSDREAQHAAGVALVLVQEPAEAVT
                     RLEAAAGQSREAPSGQSREAAAGQTNDPKVLSDLAAARYAAASQLGRASLYPTALAAA
                     DAALRLDPNLSEALFNRALTLDRLGLAFDARRAWQRYLQADGSSAWAVEAQARLAQLP
                     ATTHKAQFEHDRPLLEQAAARGDAAAVRRYVDANRELARRYAEPIYLGHWGEAVQRNE
                     MAEAARLLSISRGVGSALFAISGDALLRDAVHAIDTATPAGQQAIAAAHVLYLSGRTA
                     YSRDQRDTAERDLLRAAAAFEAARDPMALQAQYYAASVRLAGNDTAGARADLERMLAA
                     AGAHRDYISFGAYVRWDLGRALSADDDWSGAAAVLAEGAAMLQQVGEREGEAFVETNL
                     ANALSSLGRNDDAWLARMRAFSALSAEGINPQRLATAIAAAAAGELAAGHRDAALALM
                     STEASVAPDAGRPQMAISGLVTRAMLQSMNGDPSAAAQTARQADALAQQTSDPALRAS
                     WTATAGVARGAVLAAQDPRAAAEPLTRSIDFYASHATPGALLDPLLLRSRCAVRTGDV
                     PSAMRDLEQGM"
BASE COUNT          269 a          724 c          739 g          283 t
ORIGIN      
        1 catcccctgc tcgaggtcac gcatggcgga cggcacgtct ccggtgcgca cggcgcagcg
       61 cgagcggagg agcagcggat cgagaagcgc cccgggcgtc gcgtgcgacg cgtagaagtc
      121 gatgctccgc gtcagcggct cggccgcggc gcgggggtcc tgcgcggcca gcaccgcgcc
      181 cctggcaacg ccggcggtgg ccgtccagct cgcgcgcaat gccgggtcag acgtctgctg
      241 ggccagcgca tcggcttgcc gcgccgtctg cgccgccgcg gacggatcgc cgttcatcga
      301 ctgcagcatg gcccgggtga ccagtccgct gatggccatc tgcggtctgc cggcatcagg
      361 tgcgacggac gcctccgtcg acatcagcgc aagcgcggcg tcgcgatgtc cggccgccag
      421 ctcaccggcc gcggccgcgg caatggccgt ggccaggcgt tgcgggttga tcccttccgc
      481 gctgagggcg gagaaggcgc gcatccgtgc cagccaggcg tcgtcgttgc gtccgagaga
      541 ggagagggcg ttggcgagat tcgtttcaac gaaggcctcc ccttcccgct cgcccacttg
      601 ctggagcatc gccgctcctt cggccagcac ggccgcggcg ccggaccagt cgtcgtccgc
      661 actcaaggcc cggccgaggt cccagcgcac gtaggctccg aagctgatgt agtcgcgatg
      721 ggcgccggcg gcggccagca tccgctcgag gtccgcccgc gcgccggcgg tgtcgttccc
      781 ggccagccgt acgctggcgg cgtagtactg tgcctggagc gccatcgggt cgcgtgcggc
      841 ctcgaatgca gccgccgcgc ggagcaggtc acgctcggcg gtgtcacgct ggtcgcggct
      901 gtaggcagtg cggccgctca gatacaggac atgcgccgcg gcgatggcct gctgcccggc
      961 gggcgtcgcg gtatcgatgg cgtgcacggc gtcgcgcagc agcgcgtctc cggagatcgc
     1021 gaacagcgcg ctcccgacac ctcggctgat ggagagcagc cgcgccgcct cggccatctc
     1081 gttgcgctgc accgcctcgc cccagtgtcc gaggtagatc ggttcggcgt agcggcgggc
     1141 cagctcgcga ttggcgtcga cgtagcgccg cacggcggcg gcgtcaccgc gcgcggccgc
     1201 ctgctcgagc agcggacggt cgtgctcgaa ctgcgccttg tgcgtcgtcg ccggcagttg
     1261 cgccaggcgc gcctgcgcct cgactgccca ggcggaggac ccatccgcct ggagatagcg
     1321 ctgccaggct ctcctggcgt cgaacgctaa tccgaggcgg tcgagggtga gcgcgcgatt
     1381 gaagagcgct tcggaaaggt ttggatcgag acgcagtgcg gcgtcggccg ccgcgagagc
     1441 ggtcggatac agcgacgccc gcccgagctg cgaagccgcg gcgtagcgcg ccgcggcgag
     1501 gtcactcaag acttttgggt cgtttgtctg cccggccgcc gcctcacggg actgcccgga
     1561 cggcgcctca cgcgattgtc cggctgccgc ctccagacgg gtgaccgcct cggcgggctc
     1621 ctgcacgagc accagggcca cgccggcggc gtgttgtgct tcacggtcgc tgttgcgggc
     1681 ggccccttcg acgatctccc ctgccgcacc tccgagtttc aactgcgcgg cgtccttcga
     1741 ttcaccggca gcgcggtgca ctccggcata cgccgcccag gggaagccgc cggtcaggcg
     1801 aggctcgacg aggcgcgccg aacgcggcgc cagcgcaacg aggcggtgga ttgacccacg
     1861 gccgcgcagc aacaacaacg gcacggagat gagcgccagc agcacggccg ccgcaacggc
     1921 cagccaccag cgcgatgcag gtgcgggcgt agcgcctgcg ccgtgcgccg cgccgtcttc
     1981 acggagctgc gcattcgccg gcagaacggc ggtct
//
LOCUS       DASRDG010000389         5523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1320008, whole genome shotgun sequence.
ACCESSION   DASRDG010000389 DASRDG010000000
VERSION     DASRDG010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320008"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /gene="atzF"
                     /locus_tag="VFA35_06420"
     CDS             complement(<1..1579)
                     /gene="atzF"
                     /locus_tag="VFA35_06420"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HZT55841.1"
                     /translation="MEPSPLLTLADWRRAYRDGASPRALLEALRGRLLEASPPEAWIT
                     LAGAAEIAASIAALEFRASRHADRAAALRALPLFGVPFAVKDNIDVAGLPTTAACPAS
                     ARTPMAHAHAVAKLIAAGAVCMGKTNLDQFATGLVGARSPYGRPSSAFAAERISGGSS
                     SGSAVVVARGDVAFALGTDTAGSGRVPAGFNNLVGLKPTPGRVGSSGVVPACRSLDCI
                     SVFALTVADAAEVLALIEGPDAGDAYSAFRLGAAHLKAAPGAALRIGVPAAPVFGGDA
                     GYAPAFDAAIAQARALGHTVVPIDFAPLFAVAKLLYSGPWVAERNTVVQALMHSDPDA
                     IDATVRRVIEAANGFSATDAFRAQYLLRDAQRDTAALWLGVDSLDLLMVPTAPAHPTH
                     AEIDADPLGANALLGTYTNFVNLLGWCALALPAGFTARGLPFGVTFIAPGAADAALAR
                     FGQGWEASVALPVGATGRAHSSAAADPPAAWPASRPTLPIAVVGAHLAGLPLNGQLTE
                     RGATLREATTTAEHYRLF"
     gene            complement(1816..2589)
                     /locus_tag="VFA35_06425"
     CDS             complement(1816..2589)
                     /locus_tag="VFA35_06425"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HZT55842.1"
                     /translation="MSAFNEETVLSVHHWTDKLFTFTTTRDPALRFSNGHFTMIGLRV
                     NNKPLLRAYSIVSANYEDHLEFLSIKVPDGPLTSKLQHIQVGEKIIVGKKPTGTLVID
                     YLLPGKRLYLLSTGTGLAPFMSIIRDPETYERFEQVILVHGVRTKDELAYHDLVTEHL
                     PAHEFLGDMVSSKLRYYPTVTREAYRNMGRVTELMESGKLFTDLGVPNLDAAHDRVMI
                     CGSPGMLRDLKHMLEGRGFKEGNTSTPGAFVIERAFAEQ"
     gene            2728..4404
                     /locus_tag="VFA35_06430"
     CDS             2728..4404
                     /locus_tag="VFA35_06430"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZT55843.1"
                     /translation="MPTDIEIARAATLQPITTLARDRLGIPEDALDPYGRHKAKLSMA
                     YIRSLADRPAAKLILVSAINPTPPGEGKTTTTVGLGDALNRIGQSAIVCLREPSLGPV
                     FGLKGGAAGGGRAQVVPMEDINLHFTGDFNAIGLAHNLLAALIDNHVHHGNALGLDVR
                     RITWRRVVDMNDRALRQITVGLGGPANGYPREDGFDIVVASEVMAILCLADDLADLKR
                     RLGRIVVGYTRERVPVTAAQLQAHGAMAALLRDAIRPNLVQTLENNPAFIHGGPFANI
                     AHGCSSVIATRTALKLADYVVTEAGFGADLGAEKFVDITCRKSGLRPDVVVLVATVRA
                     LKYHGGAHPQALKREDLAGLAAGIANLERHVNNVRNHYGLPCVVAVNHFADDTEAELA
                     LLARKMAHHEVPVIVARHWAEGGAGAEALAREVVRIAQGTPADFRFVYPDEMTLWQKA
                     EAVATQIYGAAEVVADPRVRERLQELQDAGYGHLPICIAKTPYSFSTDPLLRGAPSGH
                     ALNIREVRLAAGAEFVVLVCGDVMTMPGLPKLPAAAAIDVDDDGNIVGLF"
     gene            complement(4424..5043)
                     /locus_tag="VFA35_06435"
                     /pseudo
     CDS             complement(4424..5043)
                     /locus_tag="VFA35_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447334.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(5079..5501)
                     /locus_tag="VFA35_06440"
     CDS             complement(5079..5501)
                     /locus_tag="VFA35_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HZT55844.1"
                     /translation="MPGQRSDPQKFAVEVKPSRIDGQGAFAAEAIPARRKIGEIRGEA
                     ISVREARRRAKGVARIMIVELSDKRAIDASNSTDPLRFTNHSCQPNAVLRIRQGRVEF
                     YAMRDLLAGEELTVNYGETHHEGKLRCRCGAPGCIGRL"
BASE COUNT          761 a         1942 c         2045 g          775 t
ORIGIN      
        1 cgaacaggcg gtagtgctcc gcggtggtgg tggcctcgcg caaggtggcg ccgcgctcgg
       61 tcaactggcc gttcagcggc aagccggcga ggtgcgcgcc gacgaccgcg attggcagcg
      121 tgggccggct cgcgggccag gcggcgggcg ggtcggcggc cgcgctcgaa tgcgcgcggc
      181 cggtggcgcc gaccggcagt gccacgctcg cctcccagcc ttggccgaag cgcgccagcg
      241 ccgcgtcggc cgcgcccggc gcgatgaagg tcacgccgaa cggcaggccg cgggcggtga
      301 atccggccgg cagcgcgagc gcgcaccagc cgagcaggtt gacgaagttg gtgtaggtgc
      361 cgagcagcgc gttcgcgccg agcgggtcgg cgtcgatctc ggcgtgggtc gggtgcgcgg
      421 gggcggtcgg caccatcaac aggtcgagcg aatcgacgcc cagccacagc gctgcggtgt
      481 cgcgctgcgc gtcgcgcagc aggtattgcg cgcggaacgc gtcggtcgcg ctgaagccgt
      541 tcgcggcctc gatcacgcgg cgcaccgtgg cgtcgatcgc atcggggtcg ctgtgcatca
      601 gcgcctgcac gaccgtgttc cgctcggcca cccacgggcc gctgtagagc agcttggcga
      661 ccgcgaacag cggcgcgaag tcgatcggca cgaccgtgtg gccgagcgcg cgggcctgcg
      721 cgatggccgc gtcgaaggcg ggcgcgtagc cggcgtcgcc gccgaagacc ggcgcggccg
      781 gcacgccgat gcgcagcgcg gcgccgggtg cggccttcag gtgtgcggcg ccgaggcgga
      841 acgcgctgta ggcatcgccc gcgtccgggc cttcgatcag cgccagcacc tcggccgcgt
      901 cggcgaccgt cagcgcgaac accgaaatgc agtccaggct gcggcaggcc ggcaccacgc
      961 cgctgctgcc gacgcggccc ggtgtcggct tgagcccgac gaggttgttg aagcccgccg
     1021 gcacgcggcc cgagccggcg gtgtcggtgc cgagcgcgaa cgccacgtcg ccgcgcgcga
     1081 ccacgaccgc cgagcccgag ctcgacccgc cgctgatgcg ctcggccgcg aacgcgctgc
     1141 tcggccggcc gtagggcgag cgcgcgccga ccaggccggt cgcgaattgg tcgaggttgg
     1201 tcttgcccat gcagaccgcg ccggcggcga tcagcttcgc gaccgcgtgc gcatgggcca
     1261 tcggggtgcg cgcggacgcg gggcaggccg cggtcgtcgg caggccggcg acgtcgatgt
     1321 tgtccttcac cgcgaacggc acgccgaaca gcggcagcgc gcgcagtgcc gcggcgcggt
     1381 cggcatggcg cgaggcgcgg aactcgagtg ccgcgatgct ggccgcgatc tcggccgcgc
     1441 cggccagcgt gatccaggcc tcgggcgggc tggcctcgag caggcgcccg cgcagcgctt
     1501 cgagcagcgc gcggggcgag gcgccgtcgc ggtaggcgcg gcgccagtcg gccagcgtga
     1561 gcagcgggga cggttccatg cgacgtaatg gtgcactcat tggtatacca gagagtgcgc
     1621 agcacgaagc gtgcccggcc gggccgggtt gactgcttcg gctgcggtgg ccgcggtggc
     1681 cgcggtcgaa ccggcgtgcg gtcgaggggt cgctagggtc gcgaacgcga gacggcggag
     1741 cgctgggccg ctcccgagcc ggcccgcacc cccttggggg ttcgcttggc gtactcgcca
     1801 ggcggggggc tgccatcatt gctcggcgaa cgcccgctcg atcacgaacg cacccggcgt
     1861 cgaggtgttg ccttccttga agccgcggcc ttcgagcatg tgcttcaggt cgcgcagcat
     1921 gcccgggctg ccgcagatca tcacgcggtc gtgcgcggcg tcgaggttcg gcacgccgag
     1981 gtcggtgaac agcttgccgg actccatcag ttcggtcacg cggcccatgt tgcggtaggc
     2041 ctcgcgcgtc acggtcgggt agtagcgcag cttgctgctg accatgtcgc cgaggaactc
     2101 gtgcgccggc aggtgctcgg tgacgaggtc gtggtaggcg agctcgtcct tggtgcgcac
     2161 gccgtgcacc aggatcacct gctcgaagcg ctcgtaggtt tccgggtcgc ggatgatgct
     2221 catgaacggc gcgagaccgg taccggtcga gagcaggtac aggcgcttgc cgggcagcag
     2281 gtagtcgatc accagcgtgc cggtgggctt cttgccgacg atgatcttct cgccgacctg
     2341 gatgtgctgc agcttgctcg tcagcgggcc gtcgggcacc ttgatgctca ggaactcgag
     2401 gtggtcttcg tagttggcgc tgacgatgct gtaggcgcgc agcagcggct tgttgttgac
     2461 gcgcaggccg atcatcgtga agtggccgtt cgagaaccgc agcgcggggt cgcgcgtggt
     2521 cgtgaaggtg aacagcttgt cggtccagtg gtgcacgctc agcacggttt cttcgttgaa
     2581 ggcgctcatg ggtcggttcg ggttcaaaga gcgcgaatcg taccgcgacg gcgagcacac
     2641 gaagcccgac agggcttcgg gcgagcgtgc ccgcccgacc cgccggtgtg gtgcgagtcg
     2701 ccgctgccga tggcatcatc cggccccatg cccaccgaca tcgagatcgc ccgcgccgcc
     2761 accctgcaac cgatcaccac gctcgcccgc gatcgcctcg gcatccccga agacgcgctc
     2821 gatccctacg gccgccacaa ggccaagctg tcgatggcgt acatccgcag cctggcggac
     2881 cggcctgcgg cgaagctgat cctcgtcagc gcgatcaacc cgacgccgcc gggcgaaggc
     2941 aagacgacga cgaccgttgg actgggcgac gcgctgaacc gcatcggcca gagcgcgatc
     3001 gtgtgcctgc gcgaaccctc gctcggcccg gtgttcgggc tcaaaggcgg tgcggccggc
     3061 ggcgggcgcg cgcaggtcgt gccgatggaa gacatcaacc tgcacttcac cggcgacttc
     3121 aacgccatcg gactcgcgca caacctgctc gcggcgctga tcgacaacca cgtgcaccac
     3181 ggcaacgcgc tcggcctcga cgtgcgccgc atcacctggc gccgcgtggt cgacatgaac
     3241 gaccgcgcgc tgcggcagat caccgtcggc ctcggcggcc cggccaacgg ctacccgcgc
     3301 gaagacggct tcgacatcgt cgtggccagc gaggtgatgg cgatcctgtg cctggccgac
     3361 gacctggccg atctgaagcg ccgcctcggc cgcatcgtcg tcggctacac gcgcgagcgc
     3421 gtgccggtca ccgccgcgca gctgcaggcg cacggcgcga tggcggcgct gctgcgcgac
     3481 gcgatccggc cgaacctggt gcagacgctc gagaacaacc cggccttcat ccacggcggc
     3541 ccgttcgcga acatcgcgca cggctgcagc tcggtgatcg cgacgcgcac cgcgctgaag
     3601 ctcgccgact acgtcgtcac cgaggccggc ttcggcgccg acctgggcgc cgagaagttc
     3661 gtcgacatca cctgccgcaa gtcggggctg cggccagacg tggtcgtgct cgtcgcgacc
     3721 gtgcgcgcgc tcaagtacca cggcggcgcg catccgcagg cgctgaagcg cgaagacctc
     3781 gcgggcctgg ccgccggcat cgcgaacctg gagcgccacg tcaacaacgt gcgcaaccac
     3841 tacgggctgc cgtgcgtggt cgcggtcaac cacttcgccg acgacaccga ggccgagctc
     3901 gcgctgctcg cgcgcaagat ggcgcaccac gaggtgccgg tcatcgtcgc acgccattgg
     3961 gccgagggcg gcgccggcgc cgaggcgctc gcgcgcgagg tggtgcgcat cgcgcagggc
     4021 acgccggccg acttccgctt cgtctacccc gacgagatga cgttgtggca gaaggccgag
     4081 gcggtcgcga cccaaatcta cggggccgcc gaggtcgtcg ccgacccgcg ggtgcgcgag
     4141 cgcctgcagg aattgcagga cgccggctac ggccacctgc ccatctgcat cgcgaagacg
     4201 ccgtactcgt tctcgaccga cccgctgctg cgcggcgcac cgagcggcca cgcgctgaac
     4261 atccgcgagg tgcggctggc ggccggcgcc gagttcgtcg tgctggtctg cggcgacgtc
     4321 atgacgatgc cggggctgcc gaagctgccc gcggcggcgg caatcgacgt cgacgacgac
     4381 gggaacatcg tcggcttgtt ctgaggccct cggcgggccc gcgtcagcgc tgcacgaagc
     4441 agcgttcgag ttggtgacgc ggcgcgcgct gcagcgcgct cagcagcgca tgggtcgcgc
     4501 gcgcgccggc cagcgccttg gcgaagcgct cctccacgtc ccgctgcatc cgcagcagca
     4561 ggtgcgcggt gacctcggcg tccgccagcg cccggtgcgc gcgcccggcg ctcggcaatg
     4621 cgtgcagccg cgcgagcgtg ccgagcttgt ggttctcggc gtgcgggtag agccgccgcg
     4681 ccagcagcag cgtgcaggcg aatgcgtgcg cagcgtcggc cgcgcattcc gcccgcgcca
     4741 gctccgcgtg ccagaagccg cggtcgaagg ccgcgttgtg cgcgaccagc gggcagcctt
     4801 gcgtgaagcg cgcgacctcg tgcatcacgg cgcgcgcggg cggcgcggct gcagcatcgc
     4861 gttcgtgatg ccggtcagct gcgtgatgaa cggcggcacc cacgcgccgc tgttcatcag
     4921 gctctggaag cggccgacga tctcgccgcc ctgcaccagc accgcggcga cctcggtcgc
     4981 gcggccgccg tgcgtgggcc cgaggccggt ggtttcgaag tcgatgacgg cgagcgtttc
     5041 catgcgtcgc ggattccggc ctgcgtgggc ttgatccact acagccgccc gatgcagccc
     5101 ggcgcaccgc agcggcagcg cagcttgccc tcgtggtgcg tctcaccgta gttcaccgtg
     5161 agttcctcgc cggccagcag atcgcgcatc gcgtagaact cgacgcggcc ctggcgaatc
     5221 cgcagcaccg cgttcggctg gcaagaatgg ttggtgaagc gcagcgggtc ggtcgagttc
     5281 gaggcgtcga tcgcgcgctt gtcgctgagc tcgacgatca tgatccgcgc gacgcccttc
     5341 gcgcggcgcc gcgcctcgcg cacgctgatc gcctcgccgc ggatctcgcc gatcttgcgg
     5401 cgcgcgggaa tcgcctcggc ggcgaacgcg ccctggccgt cgatgcggct gggcttgacc
     5461 tcgaccgcga acttctgcgg atcgctgcgc tggccgggca cgcgcgccgg cgccgtcgcg
     5521 gcg
//
LOCUS       DASRDG010000390         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1442364, whole genome shotgun sequence.
ACCESSION   DASRDG010000390 DASRDG010000000
VERSION     DASRDG010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442364"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..745)
                     /locus_tag="VFA35_06445"
     CDS             complement(110..745)
                     /locus_tag="VFA35_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZT55845.1"
                     /translation="MAAVIDAKRERILAVAERLFFEHGYARTTMEQIVRELGVTKPYV
                     YYYFHNKLEIYETLCWRPTVACFTALDFAPDDARPARDKLAAGLERLVRETIVNHPAA
                     FFAYREPQALRPEYQAAVLKLARHFYERLCALLEQARADGTADFDDTKVTALAACSIP
                     GFLFSWYQPDGRLPPEAMVRKLTALVLRVIGLRLPARVRARTSAIALTAPD"
     gene            complement(882..2306)
                     /locus_tag="VFA35_06450"
     CDS             complement(882..2306)
                     /locus_tag="VFA35_06450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZT55846.1"
                     /translation="MQHLSTVDASFLHLETPEMPMHVATFSLFELPKGYKGDYYEDVK
                     ALLAKRMHLAPIFHRKLAAMPFELADPVWIEDDDVDLDYHVRSLTLRKPGTMKQLETL
                     VARLHSSLLDRSRPLWEAYIIDGLQSGHVAYYWKAHHSGIDGKAGVELAKVFYDTTPE
                     VREVPPPRRTRRTDPYQLGVSELLQAAAKNAATQYTKLGKLLPEAAKAMGAVGQLLAE
                     RKPVAGERRLDLGLAPRTPFNVSITNQRSYALMSQPLDELKAVGKRAGGTVNTVVMAM
                     CASALRKFLLDRNELPEKPLIAMVPVSLRGADDNAMNNQVSAIRVDLATDIADPAERF
                     KAIHTSSEDAKGVVRVLKPVLGADVPITGAPWLISSMASLYGRSGLADRLPSTGNVCI
                     SNVPGMPDPLYMAGARMLHYYPVSIPAHGMALNITAQSYAGQMDWGITACRRALSQAE
                     ADELVENLRQGLREIEALPAVAAA"
BASE COUNT          317 a          813 c          885 g          402 t
ORIGIN      
        1 ttgtaggtcg cggcgtcctg ggcggcggcg gcgccggtgg cgagcgcggc ggcgagggcg
       61 aggacggtga gggcgggtct tgtcatcggt gtctccgtgt ctttgtgggt cagtcagggg
      121 cggtgagggc gatggccgac gtgcgcgcgc gtacccgcgc gggcagccgc aagccgatga
      181 cgcgcagcac cagcgcggtg agcttgcgca ccatcgcctc gggcggcagc cgtccgtcgg
      241 gctggtacca gctgaagagg aagcccggga tgctgcacgc ggccagcgcg gtgaccttgg
      301 tgtcgtcgaa gtcggccgtg ccgtcggcgc gcgcctgttc gagcagcgcg cacagccgtt
      361 cgtagaagtg gcgcgcgagc ttcagcacgg cggcctggta ctcgggccgc agcgcctgcg
      421 gctcgcggta ggcgaagaac gccgccgggt ggttgacgat ggtctcgcgc accagccgct
      481 cgaggcccgc ggcgagcttg tcgcgcgccg gccgggcgtc gtcgggcgcg aagtcgagcg
      541 cggtgaagca cgccaccgtc gggcgccagc acagcgtttc gtagatctcg agcttgttgt
      601 gaaagtagta gtagacgtag ggcttggtca cgcccagctc gcgcacgatc tgctccatcg
      661 tggtccgggc gtaaccgtgc tcgaagaaca gccgctcggc gaccgcgagg atgcgctcgc
      721 gcttggcgtc gatgacggcc gccatcgcgg ccgagtcggg cacgccggcg cgctgcccgc
      781 gccgcccgcc cggcccggcc ttcgggatgg accccaggaa tcccttcttc gcgctgcgct
      841 cgctgcgcgc gcgggtcatg ggtttctcgc tggtgcccac gtcaggccgc ggcgacggcc
      901 ggcagcgcct cgatctcgcg caggccctgg cgcaggttct ccaccagctc gtcggcctcc
      961 gcctgcgaca gcgcgcggcg gcacgcggtg atgccccagt ccatctgccc ggcgtagctc
     1021 tgcgcggtga tgttcagcgc catgccgtgg gccgggatcg acaccgggta gtagtgcagc
     1081 attcgcgcgc ccgccatgta gagcgggtcc ggcatgcccg ggacgttcga gatgcacacg
     1141 ttgccggtgg agggcagccg gtccgcgagc cccgagcggc cgtacagcga cgccatgctc
     1201 gagatcagcc acggcgcgcc ggtgatcggc acgtcggcgc cgagcaccgg cttgagcacc
     1261 cgcacgacac ccttggcgtc ttcggacgag gtgtggatcg ccttgaagcg ctcggccggg
     1321 tcggcgatgt cggtcgcgag atcgacgcgg atcgccgaca cctggttgtt catcgcgttg
     1381 tcgtcggcgc cgcgcaggct caccggcacc atcgcgatca gcggcttctc cggcagctcg
     1441 ttgcgatcga gcaggaactt gcgcagcgcc gaggcgcaca tcgccatcac caccgtgttg
     1501 acggtgccgc cggcgcgctt gccgacggcc ttcaattcgt cgagcggctg cgacatcagc
     1561 gcgtacgaac gctggttcgt gatcgagacg ttgaacggcg tgcgcggcgc gagcccgagg
     1621 tcgagccggc gctcgccggc caccggcttg cgttcggcga gcagctggcc gaccgcgccc
     1681 atcgccttgg ccgcctcggg cagcagcttg ccgagcttgg tgtactgcgt cgcggcgttc
     1741 ttcgcggcgg cctgcagcag ctccgagacg ccgagctggt acggatcggt gcggcgcgtg
     1801 cgccgcggtg gcggcacctc gcgcacctcg ggcgtcgtgt cgtagaacac cttggcgagc
     1861 tcgaccccgg ccttgccgtc gatgccgctg tgatgcgcct tccagtagta ggcgacgtgg
     1921 ccgctttgca ggccgtcgat gatgtaggcc tcccagagcg ggcggctgcg gtcgagcagg
     1981 ctcgagtgca gccgcgcgac cagcgtctcg agctgcttca tcgtgcccgg cttgcgcagc
     2041 gtcaggctgc gcacgtggta gtcgaggtcg acatcgtcgt cctcgatcca caccgggtcg
     2101 gccagctcga acggcatcgc cgcgagcttg cggtggaaga tcggcgccag gtgcatgcgc
     2161 ttggcgagca gcgccttcac gtcttcgtag tagtcgccct tgtagccctt gggcagttcg
     2221 aacagcgaaa aggtggcgac gtgcatcggc atctcgggcg tctcgagatg caggaacgac
     2281 gcgtcgacgg tgcttaggtg ttgcatcgat gtctccgatg gagatcagcg cgcggcgcgc
     2341 gcgcgttcct cttcctcgac cagttccttc ctcgacagct tgccgatcgc cgtcttgggc
     2401 agcgccgcgc ggatctc
//
LOCUS       DASRDG010000391         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1665430, whole genome shotgun sequence.
ACCESSION   DASRDG010000391 DASRDG010000000
VERSION     DASRDG010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665430"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFA35_06455"
     CDS             complement(<1..223)
                     /locus_tag="VFA35_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZT55847.1"
                     /translation="MGPSTGQGRAGITDPNLTPLIDVSLVLVVILMVATPMVFQSGIA
                     IQTAGTAGRAAAAPTREDRIELTVLPNHQL"
     gene            complement(225..872)
                     /locus_tag="VFA35_06460"
     CDS             complement(225..872)
                     /locus_tag="VFA35_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZT55848.1"
                     /translation="MFENFDWIGAMRTSPIMVIILISSIVTTGFALERAWYFWQRRKR
                     PDRVLATAFDQVRAGKVAEAAKTCARTNHPAGAVAEAVLGSLHLSTVEIEEKLQIALS
                     EQRLLLERNLGVLGTMGSTAPLIGLLGTVWGIMRAFHDMASTGSAGPSVVAAGVAEAL
                     FTTAAGLLVAVPSVLFYNQFVRRIAVMLTVAENQARHLRLAARDQAGATRREEAA"
     gene            complement(903..>3375)
                     /locus_tag="VFA35_06465"
     CDS             complement(903..>3375)
                     /locus_tag="VFA35_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT55849.1"
                     /translation="LLGERPQQPAAAYVRALALRQQGALLKAAAGFQDVADRWPESPL
                     RDAARLAKADAFLAAHQDRSAAEEFARVAARAGDDGVKAEAELRAAGAVLLIGAPDSA
                     LALFRGIVARRPGTDVAARAQFLIGQTLADAGHYPEAIVELNQVLSHYFQHSVAASAQ
                     YRVARCLDAVGRSADATGSYQAVVSGYPLEPEAPAAAYLAGVGLLHQGRPRAAAPYFQ
                     IVLDRYAARRDSAGAVVFARPEHAEMVDAALCMLEVAWHRAGDLGQLAGAPHLLLQRM
                     PPSRSPWRAWALLFDADALAAQGRFDDAQASLERIASEFRDHPAAASATQLLAWTYGQ
                     QGRDSLAVATEERLLARWAAVAPQAIVSAALLDIAHERFNQKRYLDAAAAYEDFLHRY
                     PGHPQRLTALYQAGLCYLRLDRAGDAVDRWETIVRDSAASPLAERAWARAGDAYFQAD
                     RFDDATRSYRGLLEHFGSSPAAALASLRLGQCAYNAGHDAQALEAFGVTIQQYAGTPI
                     AREAARGQELALYRLSQRPDGEAQLAKLVEQYPSSPFTADAMFQIARRAWQAKRWDEA
                     AEGFRKVVSQFPGHSAAQQAQFLMADALANGGHADDARGAYEQFLSFFPESELAPSAA
                     FRLGLLQFAAKSYAQAGVAFLRALDDSAAADVRSAALYDLAICQRLTGDTASARAGLE
                     RHRAEFPSDARSADVACQLGDLDESASAWPAAAAEYQRGLDAHPAPALATELAFRLGR
                     VREQQADVDGALRAYLLAANASDRNQAFRLSAVARLAALYETRHQTTRAVAAYRDLMA
                     NSRDHELAAAAAGRVAQLEAGAHSR"
BASE COUNT          435 a         1241 c         1266 g          433 t
ORIGIN      
        1 ggagctggtg gttcggcaac acggtcagct cgatgcggtc ctcgcgcgtc ggcgccgcgg
       61 ctgcgcgccc ggccgtaccc gcggtctgga tggcgatgcc ggactggaac accatcggcg
      121 tcgcgaccat gaggatcacc accagcacca gcgacacgtc gatgagcggc gtgaggttgg
      181 gatcggtgat gccggcgcgg ccctggcccg tggacggtcc catgtcaggc cgcctcctcc
      241 cggcgcgtcg cacccgcctg gtcgcgggcg gccagccgca ggtgccgcgc ctggttctcg
      301 gccaccgtca gcatgaccgc gatgcggcgc acgaactggt tgtagaacag caccgacggc
      361 accgcgacga gcaggccggc ggcggtcgtg aacagcgcct cggcgacgcc ggccgcgacc
      421 acggacggcc cggccgaacc ggtgctcgcc atgtcgtgga atgcgcgcat gatgccccac
      481 accgttccga gcaggccgat gagcggtgcc gtgctgccca tggtcccgag cacgccgagg
      541 ttccgctcga gcagcaggcg ctgttcgctc aacgcgatct gcagcttctc ctcgatctcc
      601 accgtcgaga ggtggaggct gccgagcacg gcttccgcca ccgcgccggc ggggtggttc
      661 gtgcgcgcgc acgtcttcgc cgcctcggcg accttgcccg cccgcacctg gtcgaacgcc
      721 gtcgccagca cgcggtccgg gcgcttccgc cgctgccaga agtaccaggc gcgctcgagc
      781 gcgaacccgg tcgtgacgat gctgctgatc aggatgatca ccatgatcgg actcgtgcgc
      841 atcgcgccga tccagtcgaa gttctcgaac atgcttcctc cccgggggtg gtggacggcg
      901 gatcagcgcg agtgcgcgcc ggcttccagc tgcgcgacgc ggccggcggc ggccgccgcg
      961 agctcgtggt cgcgcgagtt cgccatgagg tcgcggtagg ccgcgacggc gcgcgtggtc
     1021 tggtgcctgg tttcgtagag cgccgcgagc cgggccacgg cggacagtcg gaacgcctgg
     1081 ttgcggtcgc tggcgttcgc ggcgagcagg taggcgcgca gcgcgccgtc gacgtccgcc
     1141 tgctgttcgc gcacgcgccc gagccggaac gccagctcgg tggcgagcgc cggcgcggga
     1201 tgggcgtcga ggccgcgctg gtactcggcg gccgccgccg gccacgcgct cgcgctctcg
     1261 tcgaggtcgc ccagctggca ggccacgtcc gcgctgcgcg cgtcggaggg gaactcggcg
     1321 cgatgccgct cgagcccggc gcgggccgag gcggtgtcgc cggtcaaccg ctggcagatc
     1381 gccaggtcgt agagtgccgc cgagcgcacg tcggccgcgg cgctgtcgtc cagcgcccgc
     1441 aggaacgcga ccccggcctg cgcgtaggac ttggccgcga actgcagcag gccgagccgg
     1501 aacgccgcgc tcggcgccag ctcgctctcg gggaagaacg acaggaactg ctcgtacgcg
     1561 ccacgcgcat cgtcggcgtg gccgccgttc gcgagcgcgt ccgccatcag gaactgcgcc
     1621 tgctgcgcgg ccgagtgacc cgggaactgg ctcaccacct tgcggaagcc ctccgcggcc
     1681 tcgtcccagc gtttcgcctg ccacgcgcga cgggcaatct ggaacatcgc gtcggcggtg
     1741 aacgggctcg acgggtactg ttcaacgagc ttcgcgagct gcgcctcgcc gtccggacgc
     1801 tggctcagcc ggtagagcgc cagctcctgt ccgcgtgcgg cctcgcgcgc gatgggcgtg
     1861 cccgcgtact gctggatcgt gaccccgaac gcctcgagcg cctgcgcatc gtgcccggcg
     1921 ttgtaagcgc actggccgag ccgcagcgag gcgagcgcgg ccgccgggct ggacccgaag
     1981 tgctccagca ggccgcggta gctgcgcgtg gcgtcgtcga agcggtcggc ctggaagtag
     2041 gcgtcgccgg cgcgcgccca cgcgcgctcg gcgagcggcg acgcggcgct gtcgcgcacg
     2101 atggtctccc agcggtccac ggcgtcgccg gcgcgatcga gccgcaggta gcagagcccc
     2161 gcctggtaga gcgcggtcag ccgctgcggg tgccccggat agcggtgcag gaagtcctca
     2221 taggcggcgg cggcatcgag gtagcgcttc tggttgaagc gctcgtgcgc gatgtcgagc
     2281 agcgcggcgc tgacgatggc ctgcggcgcg accgccgccc agcgggccag cagccgctcc
     2341 tcggtcgcca ccgcgaggct gtcgcgcccc tgctgtccgt aggtccacgc caggagctgc
     2401 gtcgccgagg cggccgcggg atgatcgcgg aactcgctcg cgatccgctc cagcgaggcc
     2461 tgcgcgtcgt cgaaacggcc ctgcgcggcg agcgcgtcgg cgtcgaacag cagcgcccac
     2521 gcccgccacg gcgagcggct cggcggcatg cgctggagca gcaggtgcgg cgcgccggcg
     2581 agctggccga gatcgccggc gcgatgccac gcgacctcca gcatgcacag cgccgcgtcc
     2641 accatctcgg cgtgctcggg ccgcgcgaac accacggcgc cggcgctgtc ccgccgcgcg
     2701 gcgtagcggt cgagcacgat ctggaagtag ggcgccgccg cgcgcggacg accctggtgg
     2761 aggagcccca cgccggcgag gtacgcggcc gccggcgcct cgggctcgag cggatagccc
     2821 gacaccaccg cctggtagct gccggtcgca tccgccgagc ggcccacggc gtcgaggcag
     2881 cgcgcgacgc gatactgcgc gctcgccgcc accgaatgct ggaaatagtg gctgagcacc
     2941 tggttgagct cgacgatcgc ctcggggtag tggcccgcat cggcgagcgt ctgcccgatc
     3001 aggaactggg cgcgggcggc cacgtccgtg ccggggcgcc gcgccacgat gccgcggaag
     3061 agcgccagcg ccgagtccgg cgcgccgatg agcagcaccg cgcccgcggc gcggagctcg
     3121 gcctcggcct tgacgccgtc gtcgccggcg cgagcggcga cgcgggcgaa ctcctccgcg
     3181 gcgctgcggt cctggtgtgc ggccaggaac gcgtccgcct tcgccagccg ggccgcatcg
     3241 cggagcggcg actccggcca gcggtcggcc acgtcctgga atccggccgc ggccttgagc
     3301 agcgcgccct gctggcgaag cgccagcgcg cgcacgtacg ccgcggcagg ctgttgcggc
     3361 cgctcgccga gcagc
//
LOCUS       DASRDG010000392         5401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_631344, whole genome shotgun sequence.
ACCESSION   DASRDG010000392 DASRDG010000000
VERSION     DASRDG010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631344"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFA35_06470"
     CDS             <1..652
                     /locus_tag="VFA35_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55850.1"
                     /translation="WEGLLTTLQLLAMALVLGLLGALPLGVMRASSRPWLWAPVWFYT
                     WVIRGTPLLVQLFVLYYGLAQFDAVRASIAWPWLSNAWFCAVLAFALNTCAYTTEMLA
                     GAIRATPHGELEAARAFGMSWATLMRRIVLPSALRRCLPAYGNEVIMMLHATSLASVV
                     TLLDLTGAAREMNSRFYLPFEAFVTAALFYLALTFALVAGFRRAEARWLAHLQPRR"
     gene            671..1789
                     /locus_tag="VFA35_06475"
     CDS             671..1789
                     /locus_tag="VFA35_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HZT55851.1"
                     /translation="MQIRHHPLAGAPLGTQREIVSFHHGPQDTGRKVYVQASLHADEL
                     PGMLVAHHLRQRLLALEGAGRLRGEVVVVPVANPIGIGQTVLLANEGRFELGSGENFN
                     RHYPALFEPVAARLEGRLGGDAGANAQLIRAALRASIAALPEDTELISQRKTLLGLAC
                     DAEVVLDLHCDAESLLHLYTGTALWPQAEPLARLTGARASLLADESGDNPFDEACAQT
                     WWQLEAHFRGRHPIPMGCLSVTLELRGETDVSHRMASADADALVAFLTHRGIVDGLPP
                     VLPPLREPATPLAGTDVLKAPVSGVVVYVRELGEVIRAGEVVFEIVEPISGVVHPVAS
                     RADGLFFARESQRYARAGRALAKVAGAVAVRSGKLSSD"
     gene            1789..2556
                     /locus_tag="VFA35_06480"
     CDS             1789..2556
                     /locus_tag="VFA35_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT55852.1"
                     /translation="MTEKLRVENLHKRFGQVEVLKGVSLSAHAGDVIAIIGSSGSGKS
                     TLLRCINLLEQPQAGRIVLAGEELRLKAGRHGELHAADARQLQRLRSRLAMVFQHFNL
                     WSHMTALENIVEAPIRVLGVARDEAVARAEALLARVGVAHRKHLYPSQLSGGEQQRVA
                     IARALAMAPEVMLFDEPTSALDPELVGEVLRVMRDLAAEGRTMIVVTHEMGFAREVAN
                     HLVFLHQGVIEEQGVPRDVLAGPRSERLRGFLAGNLK"
     gene            complement(2586..2789)
                     /locus_tag="VFA35_06485"
     CDS             complement(2586..2789)
                     /locus_tag="VFA35_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55853.1"
                     /translation="MNKRDIKPTPPTTDRRAGSDRRRADVPLPAGQRDRRRGLEARKP
                     EVVEIDMTQSEWAALTGLPPAKK"
     gene            2993..4042
                     /gene="gnd"
                     /locus_tag="VFA35_06490"
     CDS             2993..4042
                     /gene="gnd"
                     /locus_tag="VFA35_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HZT55854.1"
                     /translation="MQLGMIGLGRMGANMVRRLMKGGVACVVFDRAAVAVRSLAADGA
                     TGAASCADLVARLAPPRAVWLMVPAAAVDATIDELLPLLAPGDTLIDGGNSYYVDDIR
                     RAQALAQRQIHCVDVGTSGGVWGLERGYCMMIGGEAEVVQRLDPVFRTLAPGVGDIAR
                     TPGRDGRGTAEQGYLHCGPNGAGHFVKMVHNGIEYGMMAAYAEGLAILKGANIGNATH
                     AVDAETTPMRDPAHYRYDMPLHDIAEVWRRGSVIASWLLDLTANALNEDPTLAKFGGR
                     VSDSGEGRWTIQAAIDEAVPAPVLTAALYARFSSRGEAEFQNRILSAMRFQFGGHVEK
                     GGGVPNPKVNDGGIR"
     gene            4146..4745
                     /locus_tag="VFA35_06495"
     CDS             4146..4745
                     /locus_tag="VFA35_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55855.1"
                     /translation="MKNVVRALALLMAAPFGLVSAAQEEAPSKSPRPRVFDARLLEQY
                     PALVDSLTAKEKNCGTDDPCAVEVTVSFVSRGGKDYCLAQFPSKIYWNRNNPPPGSRR
                     VEWTLVPDAGVTRTFSFDADLGILPIKNKGQLSSNPNDHGVVPSTDGKTYFYVHSNGL
                     SGVTHPVKYLSIVWLNDPNGTPSSTTCGTIDPIIANDGS"
     gene            complement(4751..>5401)
                     /locus_tag="VFA35_06500"
     CDS             complement(4751..>5401)
                     /locus_tag="VFA35_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT55856.1"
                     /translation="LHLARGQHVLARSHYDQALSIAEEVGDRRWEGSARSNLGLLCQE
                     LGDADQAFDHFSRALVIAKEIGDIRLEYVLQCNLGIFFGKQSEHAKAIAYLRAAAQAA
                     QSNNDLRSEGQFRGYLAVTLARLAQFADATLAWNTAKSLLSTVSDRLSMALLTSQGAE
                     IQLLSGNIVNAAALIASASLEAQELRVEAGSELATAIDAVKERLRSGNAGIQGTST"
BASE COUNT          820 a         1843 c         1879 g          859 t
ORIGIN      
        1 ctgggaaggc ctgctgacga ccctgcagct gctcgcgatg gcgctcgtgc ttggcctgct
       61 cggcgcgctg ccgctggggg tgatgcgggc gtcgagccgc ccatggctct gggcgccggt
      121 ctggttctac acctgggtca tccgcggcac accgctgctg gtgcagctgt tcgtcctcta
      181 ctacggcctc gcgcagttcg acgcggtgcg cgccagcatc gcctggccct ggctctcgaa
      241 cgcatggttc tgcgcggtgc tcgcgttcgc gctgaacacc tgcgcctaca cgaccgagat
      301 gctcgccggc gcgatccgcg cgacgccgca cggcgagctc gaggctgcgc gcgcgttcgg
      361 catgtcgtgg gccacgctga tgcggcgcat cgtgctgcct tcagcgctgc gccgctgcct
      421 gccggcctac ggcaacgagg tcatcatgat gctgcacgcg acctcgctcg cgagcgtcgt
      481 gaccctgctc gacctgaccg gcgcggcgcg cgagatgaac tcgcgcttct acctgccgtt
      541 cgaggccttc gtcaccgcgg cgctgttcta cctggcgctg accttcgcgc tggtcgcggg
      601 cttccgccgc gccgaggcgc gctggctcgc gcacctgcag ccgcggcgct gacaccaccg
      661 gggcacgtgc atgcagatcc gacaccaccc gctcgccggc gcgccgctcg gcacgcagcg
      721 cgagatcgtc agcttccacc acggtccgca agacacgggc cgcaaggtct atgtgcaggc
      781 ctcgctgcat gccgacgaac tgcccggcat gctggtcgcg caccacctgc ggcaacggct
      841 gctcgcgctc gaaggcgcgg ggcggctgcg cggcgaggtt gtcgtcgtgc cggtcgcgaa
      901 cccgatcggc atcggccaga ccgtgctgct cgcgaacgaa ggccgcttcg agctcggcag
      961 cggcgagaac ttcaaccgcc actacccggc actgttcgag ccggtcgcgg cccggctcga
     1021 aggacggctc ggcggcgatg ccggcgcgaa cgcgcaactc atccgcgccg cgctgcgagc
     1081 gtcgatcgcc gcactgcccg aagacaccga actgatcagc cagcgcaaga cgctgctcgg
     1141 cctcgcctgc gacgccgagg tcgtgctcga cctgcattgc gatgccgagt cgctgctgca
     1201 cctgtacacc ggcaccgcgc tgtggccgca ggccgagccg ctcgcgcggc tgaccggcgc
     1261 gcgcgcgtcg ctgctcgccg acgagtcggg cgacaacccg ttcgacgagg cctgcgcgca
     1321 gacctggtgg cagctcgaag cgcactttcg cggccggcat ccgatcccga tgggctgcct
     1381 cagcgtgacg ctggagttgc gaggtgagac cgacgtgtcg caccgaatgg cttcggccga
     1441 tgcagatgcc ctggtcgcgt tcctcaccca ccgcggcatc gtcgacggcc tgccgcccgt
     1501 gctgccgccg ctgcgcgaac cggcgacgcc cttggccggc accgacgtgc tgaaggcgcc
     1561 ggtcagcggc gtcgtcgtct atgtgcgcga actgggcgag gtgatccgcg ccggcgaggt
     1621 cgtgttcgag atcgtcgagc cgatcagcgg cgtggtgcac ccggtcgcga gccgcgccga
     1681 tggcctcttc ttcgcgcgcg agtcgcagcg ctacgcgcgc gccggccgcg cgctcgcgaa
     1741 ggtggccggt gcggtcgcgg tgcgcagcgg caaactgtcg agcgactgat gaccgagaag
     1801 cttcgggtcg agaatctgca caagcgcttc ggccaggtcg aggtgctcaa gggcgtgagc
     1861 ctgagcgcgc acgccggcga cgtgatcgcg atcatcggtt ccagcggctc gggcaagagc
     1921 acgctgctgc gctgcatcaa cctgctcgag cagccgcagg ccgggcgcat cgtgctcgcc
     1981 ggcgaggaac tgcggctgaa ggccgggcgc cacggcgaac tgcatgccgc cgatgcgcgg
     2041 cagctgcagc gcctgcggtc gcggctggcg atggtgttcc agcacttcaa cctgtggtcg
     2101 cacatgacgg cgctcgagaa catcgtcgag gcgccgatcc gcgtgctcgg cgtggcgcgc
     2161 gacgaagcgg tcgcgcgcgc cgaggcgctg cttgcgcgcg tcggcgtcgc gcaccgcaag
     2221 cacctgtatc cgtcgcagct ctccggcggc gagcagcagc gcgtcgcgat cgcgcgcgcg
     2281 ctcgcgatgg cgcccgaggt gatgctgttc gacgagccga cctcggcgct cgacccggaa
     2341 ctcgtcggcg aggtgctgcg cgtgatgcgc gacctggccg ccgagggccg cacgatgatc
     2401 gtcgtcacgc acgagatggg cttcgcgcgt gaggtcgcga accacctggt gttcctgcac
     2461 cagggcgtga tcgaggagca gggcgtgccg cgcgacgtgc tggccgggcc gcgcagcgag
     2521 cggctgcggg gctttctggc ggggaacctg aagtagccgg aggccgcgcc ggcttcgggg
     2581 ctggcctatt tctttgccgg tggcaggccg gtgagcgcgg cccactccga ctgggtcatg
     2641 tcgatctcga cgacctcggg cttgcgcgcc tcgagcccgc ggcggcggtc gcgctggccg
     2701 gccggcagcg gcacgtccgc acgccgacgg tcgctgccgg cgcgccggtc ggtcgtcggc
     2761 ggggtcggtt tgatgtcgcg cttgttcatg gcggggccat tctaggcagg cgcgcgcgcc
     2821 accgtgcgca agaccttcgg cgccacggcc gccagggcgt gaactcgtcc tcacgacaga
     2881 cgatgtcggc cacgcgggca tgcggtcgcg cgacaccgcg gcttcaccca aggatcgcgc
     2941 ggcggaacta cgatgcgcca tccgcgccgc gggcgcgctg ctggagcgag ccatgcaact
     3001 cggaatgatc ggcctcggcc gcatgggtgc gaacatggtg cggcgcctga tgaaaggcgg
     3061 cgtggcgtgc gtggtgttcg atcgcgcggc cgtggcggtg cgctcactcg ccgccgatgg
     3121 cgcgaccggc gccgcctcgt gcgccgacct ggtcgcccgg ctggcgccgc cgcgcgcggt
     3181 gtggctgatg gtaccggccg cggcggtcga cgcgacgatc gacgaactgc tgccgctgct
     3241 cgcgcccggc gacacgctga tcgacggcgg caactcgtac tacgtcgacg acatccgccg
     3301 cgcgcaggcg ctggcgcaaa ggcagatcca ctgcgtcgac gtcggcacca gcggcggcgt
     3361 gtgggggctg gagcgcggct actgcatgat gatcggcggc gaggccgagg tggtgcagcg
     3421 gctcgacccg gtgttcagga cgctggcccc cggcgtcggc gacatcgcgc gcacccccgg
     3481 tcgtgacggc cgcggcaccg ccgagcaagg ctatctgcac tgcggcccga acggcgcggg
     3541 gcacttcgtc aagatggtgc acaacggcat cgagtacgga atgatggcgg cctacgccga
     3601 gggcctcgcc atcctgaagg gcgcgaacat cggcaacgcg acgcacgcgg tcgacgccga
     3661 aaccacgccg atgcgcgacc ccgcgcacta ccgctacgac atgccgcttc acgacatcgc
     3721 cgaagtctgg cgccgcggca gcgtgatcgc ctcgtggctg ctcgacctga cggccaacgc
     3781 gctgaacgaa gacccgacgc tcgcgaagtt cggcggccgc gtgtccgatt ccggcgaagg
     3841 ccgctggacg atccaggccg cgatcgacga ggccgtgccg gccccggtgc tgaccgcggc
     3901 gctgtacgcg cgcttcagtt cacgcgggga ggcggagttc cagaaccgca tcttgagcgc
     3961 gatgcggttt cagtttgggg ggcatgtgga gaaggggggc ggcgtcccca atccgaaggt
     4021 caacgacggt ggaattcggt aggcaatcgt atcgacgaag agcacactcg aactcggctg
     4081 cttcgacagc aggaaccgcg ccgccgcgtg cggtcgtcgt catcgactga atcgggagaa
     4141 gcaagatgaa gaacgtcgtc cgcgctctag ctcttctcat ggcagcgccg ttcgggctgg
     4201 tcagcgccgc gcaagaggag gcgccgtcga aatccccgag gcccagggtg ttcgacgcgc
     4261 gattgctgga gcaataccct gcgctggtcg acagcctcac cgccaaggaa aagaactgcg
     4321 ggaccgacga tccttgcgcg gtcgaggtga cggtgtcttt tgtcagccgc ggcggcaagg
     4381 actactgcct ggcgcaattt ccctcgaaga tctattggaa ccggaacaac ccccctcccg
     4441 gctcgcgcag ggtggagtgg acgctggtgc cagatgccgg agtcaccaga acgttcagct
     4501 tcgacgccga tttggggata ttgccgatca agaacaaggg ccagctgagt tccaacccga
     4561 acgaccatgg cgtggtgccg tcaaccgacg ggaagaccta cttctacgtg cactcgaatg
     4621 gcttgtccgg agtcactcat ccggtcaagt atctctcgat cgtttggctc aacgacccga
     4681 acggaacgcc gtcttccacc acctgcggaa ccatcgatcc gatcattgcc aacgacggaa
     4741 gctaaagagt tcaggtgctc gtaccttgga taccggcgtt tcctgaacgg agtctctcct
     4801 tgacggcatc gatggcggtc gccaactcgg agccggcctc gacacgcagt tcctgggctt
     4861 ccaacgacgc cgaagcgatc agcgctgcgg cattcacgat gttcccggac agcagttgta
     4921 tttcggcgcc ttgcgacgtc aagagcgcca tgctcagtcg atcggaaact gtcgagagca
     4981 aggatttcgc ggtgttccag gcgagcgtcg cgtcggcgaa ctgtgcgagc cgcgccaggg
     5041 tcaccgccag atagcctctg aactggccct cggagcgcaa gtcgttgttg gactgggccg
     5101 cttgcgcggc cgctcgaaga tatgcaatgg cctttgcatg ctccgactgc ttgccgaaga
     5161 agattcccag gttgcattgc agcacgtact ccaggcggat gtctccaatt tccttcgcga
     5221 tgaccagcgc acgcgagaag tggtcaaagg cttggtcggc atcgcccagt tcctgacaga
     5281 gcaaacccaa gttgctgcgc gcgctgcctt cccaccgtcg gtctccgact tcctccgcga
     5341 tcgacagggc ttggtcgtag tggctccgcg ccaacacgtg ttgtccgcgc gcgaggtgca
     5401 a
//
LOCUS       DASRDG010000393         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_631432, whole genome shotgun sequence.
ACCESSION   DASRDG010000393 DASRDG010000000
VERSION     DASRDG010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631432"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /gene="fabD"
                     /locus_tag="VFA35_06505"
     CDS             complement(<1..867)
                     /gene="fabD"
                     /locus_tag="VFA35_06505"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZT55857.1"
                     /translation="MTTFAFVFPGQGSQAVGMLDAWGGDAAVRQTLQEASEALGEDVA
                     KLIHAGPKEQLDLTTNTQPVMLTAGIACYRAWLAETGRVPSVVAGHSLGEYTALVAAG
                     ALTLADALPLVRFRAQAMQDAVPVGVGAMAAILGMDAQAVREGCAEVAASTGEVVEAV
                     NFNDPKQTVIAGTKAGVEKACELLKAKGAKRALPLPVSAPFHSSLMKPAADRLRERLA
                     ATKFGPPSIPVINNIDVAIQSDADAIRDALFRQSFGPVRWVETVQAIRARGVTHLIEC
                     GPGKVLAGMVKRI"
     gene            complement(953..1945)
                     /locus_tag="VFA35_06510"
     CDS             complement(953..1945)
                     /locus_tag="VFA35_06510"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZT55858.1"
                     /translation="MTSNLSNRHSRITGTGSFLPPKRLTNDALAAQLALDGIETSDQW
                     IVERTGIRARHFAEPDVSSSDLAVAAARRALEAADVDAAAIDLIVVATSTPDMVFPST
                     ACLVQRKLGIAGCAAFDVQAVCSGFVYALAVADSMIRTGAASKALVIGAEVFSRILDF
                     KDRGTCVLFGDGAGAVVLEASETPGILASELHADGRHADILCVPGNVSGGQILGDPLL
                     KMDGPAVFKLAVGVLYDVAESVLEKAGLGEADVDWLIPHQANIRILQSTAKRLKLPLE
                     KLIVTVDQHGNTSAASIPLALDEAVRSGKIKPGDTVLLEGVGGGFTWGAVLFDL"
     gene            complement(1942..>2293)
                     /locus_tag="VFA35_06515"
     CDS             complement(1942..>2293)
                     /locus_tag="VFA35_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HZT55859.1"
                     /translation="SEGLASMLSKFVKQEFTRSPFTKLAALAALPVLRRLQKRVDHRR
                     YNGAALLGLRGLVFKSHGSADAFAFEQALNRAYDAARNGLLDRVHDRILESLAALPVP
                     VVAGDAAPDIARTA"
BASE COUNT          342 a          790 c          806 g          355 t
ORIGIN      
        1 gatgcgcttg accattcccg cgagcacctt gccgggcccg cattcgatga ggtgggtgac
       61 gccgcgggcg cggatcgcct gcaccgtctc gacccagcgc accgggccga aggactggcg
      121 aaagagcgca tcgcggatcg cgtcggcgtc gctctggatc gcgacgtcga tgttgttgat
      181 caccgggatc gatggcggcc cgaacttcgt cgccgcgaga cgctcgcgca ggcgatccgc
      241 agcgggcttc atcaggctcg aatggaacgg cgccgacacc ggcagcggca gcgcgcgctt
      301 cgcgcccttc gccttcagca gctcgcaggc tttctcgaca ccggccttgg tgccggcgat
      361 gacggtctgc ttcgggtcgt tgaagttcac cgcctcgacg acttcgccgg tcgacgcggc
      421 gacttcggcg caaccttcgc gcactgcctg cgcgtccatg cccaggatcg ccgccatcgc
      481 accgacgccg accggcaccg cgtcctgcat cgcctgcgcg cgaaagcgca ccagcggcag
      541 cgcatcggcc agcgtcagcg cgccggcggc gacgagtgcc gtgtactcgc ccagcgagtg
      601 gccggcgacc acgctcggca cccgccccgt ctcggcgagc caggcgcggt agcacgcgat
      661 gcctgcggtc agcatcaccg gctgggtgtt ggtggtcagg tcgagctgct ctttcgggcc
      721 ggcatggatc agcttcgcca catcctcgcc gagcgcctcg gaagcctctt gcaaggtctg
      781 ccgcacggcg gcgtcgccgc cccaggcgtc gagcatgccg acggcctgcg agccctggcc
      841 ggggaatacg aatgcgaacg ttgtcatcgc tgtgctgcgc ttgctgtcgc cagttgagcg
      901 cggtctgttg gaatgagtga gcccgggtgg ctggggtgat cgcagacagc gctcagaggt
      961 cgaacagcac cgcgccccag gtgaagccgc cgccgacgcc ttcgagcagc accgtgtcgc
     1021 cgggcttgat cttgccgctg cgcaccgctt cgtcgagcgc cagcgggatc gacgccgccg
     1081 aggtgttgcc gtgctggtcg accgtgacga tcagcttctc gagcggcagc ttgagccgct
     1141 tcgccgtgct ctgcaggatg cggatgttgg cctggtgcgg gatcagccag tcgacatcgg
     1201 cttcgccgag cccggccttt tcgagcaccg attcggcgac gtcgtacagc acgccgaccg
     1261 cgagcttgaa caccgccggg ccgtccatct tcagcagcgg atcgccgagg atctgcccgc
     1321 cggacacatt gccgggcacg cacaggatgt cggcatggcg gccgtcggcg tgcagttcgc
     1381 tcgccaggat gccgggcgtt tcgctcgcct cgagcacgac cgcgccggcg ccgtcgccga
     1441 acagcacgca ggtgccgcgg tccttgaagt cgaggatgcg cgagaagacc tcggcgccga
     1501 tcaccagcgc cttgctcgcc gcgccggtgc ggatcatcga atcggccacc gccagcgcgt
     1561 agacgaagcc cgagcagacg gcctgcacgt cgaacgcggc gcagccggcg atgccgagct
     1621 tgcgctgcac caggcacgcg gtcgacggga acaccatgtc gggcgtgctg gtcgcgacga
     1681 cgatcaggtc gatggcggcg gcatcgacat cggcggcttc gagcgcgcgc cgcgcggcag
     1741 ccaccgcgag gtcgctgctg ctcacatcgg gctcggcgaa gtggcgcgcg cgaatgccgg
     1801 tgcgctcgac gatccactgg tcggaggtct cgatgccgtc gagcgcgagc tgcgcggcca
     1861 gcgcgtcgtt ggtcaggcgc ttcggcggca ggaaactgcc ggtgccggtg atgcgtgaat
     1921 ggcggttcga gagattcgat gtcatgcggt gcgtgcgatg tccggcgcgg cgtcacccgc
     1981 gacaacaggc acgggcagcg cggccagcga ttcgaggatc cggtcgtgga cccgatccag
     2041 caggccgttt cgagcggcat cataagcccg attcagcgcc tgctcgaagg cgaacgcatc
     2101 ggcggagccg tggctcttga acaccagccc gcgcaggccc agcagcgccg cgccgttgta
     2161 gcgccggtgg tcgacacgtt tttgcaggcg ccgcagcacc ggcagcgcgg ccagtgcggc
     2221 gagcttggtg aacgggctgc gcgtgaactc ctgcttgacg aacttcgaca gcatcgacgc
     2281 caggccctcg gag
//
LOCUS       DASRDG010000394         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1666165, whole genome shotgun sequence.
ACCESSION   DASRDG010000394 DASRDG010000000
VERSION     DASRDG010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666165"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VFA35_06520"
     CDS             <1..1460
                     /locus_tag="VFA35_06520"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZT55860.1"
                     /translation="WRVQLLGESHALLALGFATIAVPLALSAQATTCTFALEGAALVW
                     LGLRQLRRVPRWIGYALQGMAGAIAGDRFAAAADALPILNGTFLGAALLALAGLVSAR
                     LLHRRDARAPLTLLFLVWGGFWWYLGGGNEIERFVVHVRHWDAWLAFLAATALIAVEA
                     SRRLAWRELLAPAAVTIALGLPFVGLTMLDEHGPLEGWHLAVWALWLLAAWRTLPALA
                     AEQLRAARILHFVYLWICVLLLALELGHLGHDHLHLRAIWFALTLLVPFAAVYLLVLR
                     RSPLVRWPLGAAADALRAPLLGSLAAAMGIAWLAGLVQEGEPTPLPYLPLLNPLELAQ
                     IAWLVLLALWYRQAQGEGKVYFDAELRARVLAAAGLVLLTTITLRCVHFFGGVPWDAG
                     MASSPLAQSALSIVWTLAGIAAMLAGKQRGSRAVWFGGAALMALVLVKLMLIDRNYLN
                     DLSAIVGVLVVGVLLVAVGYFAPVPPKMVAAGEKT"
     gene            1457..>2316
                     /locus_tag="VFA35_06525"
     CDS             1457..>2316
                     /locus_tag="VFA35_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HZT55861.1"
                     /translation="MKRLTALSVALLAIAAAAHGDESKSHAYSWPLGLAGDGAAWQVE
                     LPLEVYQTLTDAQLRDLVVVDEQGNAVPTAARTTDPATAVSARTELPLFALPAGLPSN
                     PADGPLNLRIERDADGRLRSIGADLGAANAKVASDSQDYLIDASKLDAPIDRLRLDWD
                     EAAGAVNAQFALGASDDLQNWRSAVASANVLSLAQYGNHLELHEIGAAGVQAKYLRLR
                     RLDRGAALPGLRVSVVTSAQRSPARAAREWLALTTKDAAASGTPTPAGVEYRYTLPAA
                     LPIEAIRLDLV"
BASE COUNT          281 a          846 c          777 g          412 t
ORIGIN      
        1 gctggcgcgt gcaactgctc ggtgaatcgc atgcgctgct cgcgctcggc ttcgccacga
       61 tcgcggtgcc gctcgcgctg tcggcgcagg cgacgacgtg tacattcgcg ctcgaaggcg
      121 cagcgctggt ctggctcggc ctgcgccagc tgcggcgcgt gccgcgctgg atcggctacg
      181 ccctgcaggg catggccggc gcgatcgccg gcgaccgttt cgccgccgct gccgatgcgc
      241 tgccgatcct caacggtacg ttcctcggcg ccgcactcct cgccctcgcc gggctcgtca
      301 gcgcgcgcct gctgcaccgg cgcgatgcgc gcgcgccgct gaccctgctg ttcctcgtct
      361 ggggcggatt ctggtggtac ctcggcggcg gcaacgaaat cgaacgtttc gttgtacatg
      421 tgcggcactg ggacgcgtgg ctcgccttcc ttgccgcgac cgcgctgatc gccgtcgaag
      481 cttcacgccg cctggcctgg cgcgaactgc tcgcgccggc cgccgtcacg atcgcgctcg
      541 gcctgccgtt cgtcggcctg accatgctcg acgagcacgg cccgctcgaa ggctggcatc
      601 tcgctgtgtg ggcgctctgg ttgctcgccg cgtggcgcac gctgccggcg ctcgccgccg
      661 aacaattgcg cgcggcgcgc atcctgcatt ttgtctattt gtggatttgt gtacttctgc
      721 ttgcactcga gctcggccat ctcggccacg atcacctgca cctgcgcgcg atctggttcg
      781 cgctgaccct gctcgtcccg ttcgcggcgg tgtacctgct cgtgctgcgc cgctcgccgc
      841 tcgtgcgctg gccgctcggc gccgcggccg acgcgctgcg cgcgccgctg ctcggtagtc
      901 tcgccgcggc catgggcatc gcctggctcg ccggcctcgt gcaggaaggc gagccgacgc
      961 cgttgccgta tctgcccttg ctcaacccgc ttgaactcgc gcagatcgcc tggctggtcc
     1021 tgctcgcgct gtggtatcgc caggcgcaag gcgaaggcaa ggtttacttt gacgccgagt
     1081 tgcgcgcgcg cgtgctcgcc gctgccggac tcgttttgct gaccacgatc acgctgcgct
     1141 gcgtgcactt cttcggcggc gtgccttggg acgcgggcat ggcttcgtcg ccgctcgcgc
     1201 agtcggcgtt gtcgatcgtg tggacgctgg ccggcatcgc cgcgatgctc gccggcaagc
     1261 agcgtggttc gcgcgcggtc tggttcggcg gcgcggcgtt gatggcgctc gtgctggtca
     1321 agctgatgct gatcgaccgc aactatctca acgacctgtc tgcaatcgtc ggcgtactcg
     1381 tcgtcggcgt actgctcgtc gcggtcggct acttcgcgcc ggtgccgccg aagatggtcg
     1441 ccgctggaga gaagacgtga agcgccttac cgcgttgtcc gttgccctgc tcgccatcgc
     1501 cgccgccgcg catggcgacg aatcgaaaag ccatgcgtat tcctggccgc tggggctggc
     1561 cggcgacggc gcggcctggc aggtcgagct accgctcgag gtctaccaga cgctgaccga
     1621 tgcacaactg cgcgacctcg tcgtcgtcga cgagcagggc aatgccgtgc cgaccgcggc
     1681 acgcacgacc gatcccgcca ccgccgtttc cgcgcgcacc gaactgccct tgttcgcact
     1741 gcccgccggc ctgccttcga atccggccga cggcccgctc aacctgcgca tcgagcgcga
     1801 cgcggacggg cgcctgcgca gcatcggtgc cgatctcggc gcggcgaatg caaaggtggc
     1861 ctcggactcg caggattatc tgatcgacgc gagcaagctc gacgcaccga tcgaccgcct
     1921 gcgcctcgac tgggacgagg ccgccggtgc ggtcaacgcg caattcgccc tcggcgccag
     1981 cgacgatctg cagaactggc gcagcgcggt cgccagcgcg aatgtgctca gcctcgcaca
     2041 gtacggcaat cacctcgagc tgcatgaaat cggcgcggcc ggcgtgcagg cgaaatacct
     2101 gcgcctgcgc cgcctcgatc gcggtgcggc gctgccgggc ctgcgcgtca gcgtcgtcac
     2161 ctcggcgcaa cgcagcccgg cccgcgccgc gcgcgaatgg ctcgccctca cgaccaagga
     2221 tgcggccgcc tccggcacgc cgacgccggc tggcgtcgag tatcgctata ccctgccggc
     2281 cgcgctgccg atcgaggcga tcaggctcga tctcgt
//
LOCUS       DASRDG010000395         4020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_421195, whole genome shotgun sequence.
ACCESSION   DASRDG010000395 DASRDG010000000
VERSION     DASRDG010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421195"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="VFA35_06530"
     CDS             <1..1577
                     /locus_tag="VFA35_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZT55862.1"
                     /translation="ARLADGRQRIVHLEAEPEFNEHGNLVGYSGIVQDVTDRRMAEDK
                     IRHLANFDVLTGLPNRRQLIWRAERALEHGRRLNHQVALLLIDLDRFKVINDTLGHGA
                     GDELLMEVARRLRSCVRHSDQVTESSIESLGSRSHRSLEAVGRLGGDEFVALLPEVAD
                     ERDAERVASRILDLMREPIFVGGQECFVTASVGIALYPRDGQSVADLMRNSDVAMYSV
                     KSAGRNSASLYRPALAGQGREKLELESALHKAIERDELVLHYQPKVDVRGAKMVGAEA
                     LMRWRRNGVLVAPGDFIPMAEETGLIVPLSEWAIREAARQARIWQDSFGFADSIAVNL
                     PNRLFERTDLVEHIHNAVTTYGVPHHAIELEITETGLMKDLQNVIPALHRLNEIGVEI
                     SIDDFGTGYSSLAYLTTLPISELKIDRSFVRDLGMTPQSSAVVTAIIALARSLGLRVI
                     AEGVENLRQMEVLHRLGCGVMQGFLFSRPQPPEDIEKWLQQTILPRKAPWIGRAADAE
                     VLRFAESRDPRAKPVG"
     gene            1596..3245
                     /locus_tag="VFA35_06535"
     CDS             1596..3245
                     /locus_tag="VFA35_06535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZT55863.1"
                     /translation="MESSRPAALTGSAFDAAALAKAALRRLALDKLEPTPENYAKAWR
                     HESGEPPAAAEDGLAIAALIERIVRGIERGSRGWTTARKKDSLQRVLTGSRSDAKRLQ
                     QRLTQLVASWDVDAGDAEAAAPAPASTSNDETVFAAAPVSTAGLPLEATAPSAWGRAV
                     DALGGSLQLALPPQDDAPHEIATALAALADRIGGEGATPALVDALEALCRRAAPILQH
                     RHHLLDQLNGLCTELTASLAELAENDSWAQGQCAAMRVKIDEGLSARGIRAVSELLRD
                     TRARQGRLREERERARDSLKGLINRMLAELDELGSQTGNFHDSVGRYADVIEKADSLE
                     SLTGVVREMVEESRTVQALVQQTQARLQAEHGKAQGLTQRVVELEAEMQRLSGEVSTD
                     QLTQIANRRGLLLAFDAECARLARSGSTLSVGLLDIDNFKRLNDELGHGAGDEALKSL
                     AAVVSKTLRPTDHVARYGGEEFVVLLPDTPVIEARQILTRLQRSLTGGLFMHEHKQVF
                     VTFSAGVTAHRPGERIEQALERADQGLYEAKRTGKNRTCLG"
     gene            complement(3257..3568)
                     /locus_tag="VFA35_06540"
     CDS             complement(3257..3568)
                     /locus_tag="VFA35_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55864.1"
                     /translation="MVVFFVGRSETKHLSEVAPRVGMSYDDLMAMAPRIASNTGATLG
                     TSRHVVYLLACSGRSSRATLETQAAQAGALAKQQRLSDREAVGAVLAAAGAGAASLKG
                     C"
     gene            complement(3526..>4020)
                     /locus_tag="VFA35_06545"
     CDS             complement(3526..>4020)
                     /locus_tag="VFA35_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT55865.1"
                     /translation="AQDVVFSFKRASTPKAIVSWQYPKGMKITARGPGTVVITLPTAN
                     ASFLSYLTLWGTAVVSQRYAAKVGDKGLASKPLGSGPFCLKSWQRGAEIDLSRNSYFW
                     LKDKQGNRLPYLDEVKWKIIKDDPARRGAQQAWSGTSRRSSGSGWRWPSWSSSSSAAA
                     RPST"
BASE COUNT          634 a         1450 c         1377 g          559 t
ORIGIN      
        1 atgcccgcct cgccgacggc cggcagcgca tcgtccacct cgaggccgag cccgagttca
       61 acgagcacgg caacctggtc ggctacagcg gcatcgtgca agacgtgacc gaccgccgca
      121 tggccgaaga caagatccgg cacctcgcga atttcgacgt gctgaccggc ctgccgaatc
      181 ggcgccagct gatctggcgc gccgagcgcg cgctcgaaca cggccggcgc ctgaaccacc
      241 aggtcgcgct gctgttgatc gacctcgacc gcttcaaggt catcaacgac acgctcggcc
      301 acggcgccgg cgacgagctg ctgatggagg ttgcgcgccg gttgcgctcg tgcgtgcgcc
      361 actccgacca ggtcaccgag agctcgatcg agtcgctcgg gtcgcgctcg catcgctcgc
      421 tcgaagccgt cggccgcctc ggcggcgacg agttcgtcgc gctgctgccc gaagtggccg
      481 acgagcgcga tgccgagcgc gtcgcctcgc gcatcctcga cctgatgcgc gagccgatct
      541 tcgtcggcgg gcaggagtgt ttcgtgaccg ccagcgtcgg catcgcgctg tacccgcgcg
      601 acggccagtc ggtggccgac ctgatgcgca actccgacgt cgcgatgtac tcggtcaagt
      661 cggccgggcg caattcggcg tcgctgtacc ggccggcgct ggccggccag ggccgcgaga
      721 agctcgaact cgaaagcgcg ctgcacaagg cgatcgaacg cgacgagctg gtgctgcact
      781 accagcccaa ggtcgacgtg cgcggcgcga agatggtcgg cgccgaggcg ctgatgcgct
      841 ggcgccgcaa cggcgtgctg gtggcgccgg gcgacttcat cccgatggcc gaggagaccg
      901 gcctgatcgt gccgctcagc gaatgggcga tccgcgaggc ggcgcgccag gcgcgcatct
      961 ggcaagacag cttcggcttc gccgactcga tcgcggtgaa cctgccgaac cgcctgttcg
     1021 agcgcaccga cctggtcgag cacatccaca acgcggtcac gacctacggc gtgccgcacc
     1081 acgcgatcga gctcgagatc accgagacgg gcctgatgaa ggacctgcag aacgtgatcc
     1141 cggcgctgca ccggctcaac gagatcgggg tcgagatctc gatcgacgac ttcggcaccg
     1201 gctactcgtc gctcgcgtac ctgacgacgc tgccgatcag cgaactcaag atcgaccgca
     1261 gcttcgtgcg cgatctcggc atgacgccgc agagttcggc cgtcgtcacc gcgatcattg
     1321 cgctcgcgcg ctcgctcggc ctgcgcgtga tcgccgaggg cgtcgagaac ctgcgccaga
     1381 tggaggtgct gcatcgcctc ggctgcggcg tgatgcaggg ttttctgttc agccggccgc
     1441 agccgcccga ggacatcgag aaatggctgc agcagaccat cctgccgcgc aaggcgccgt
     1501 ggatcggcag ggccgccgac gccgaggtgc tgcgtttcgc cgagagccgc gacccgcgcg
     1561 ccaagcccgt cggctgaggc cgaaccaccc gacgcatgga atcgagtcgc cccgccgcgc
     1621 tgaccggcag cgccttcgat gccgccgcgc tcgcgaaggc cgcgctgcgc cgcctcgcgc
     1681 tcgacaagct cgagcccacg cccgagaact acgcgaaagc gtggcgccat gagtccggcg
     1741 agccacccgc ggccgccgaa gacggcctgg cgatcgccgc gctgatcgag cgcatcgtgc
     1801 gcggcatcga gcgcggcagc cgcggctgga cgaccgcgcg caagaaggac agcttgcaac
     1861 gcgtgctgac cggcagccgc agcgacgcga agcggctgca gcagcgcctg acccaactcg
     1921 tcgcgagctg ggacgtcgat gccggcgacg ccgaagcggc cgcacctgcg cccgcatcca
     1981 cgtcgaacga cgagaccgtc ttcgctgctg cgccggtttc gaccgccggt ctgccgctcg
     2041 aggccacggc accatccgcc tggggccgcg ctgtcgacgc gctcggcggc agcctgcaac
     2101 tcgccctgcc gccgcaggac gacgcgccgc acgagatcgc gaccgctctt gccgcgctgg
     2161 ccgaccgcat cggcggcgag ggtgccacgc cggcgctggt cgacgcgctc gaagcgctgt
     2221 gccgccgcgc cgcgccgatc ctgcagcacc gccaccacct gctcgaccaa ctgaacggcc
     2281 tgtgcaccga gctgacggca agcctggccg agctggccga gaacgacagc tgggcccagg
     2341 gccagtgcgc ggcgatgcgc gtcaagatcg acgagggcct gagcgcgcgc ggcatccgtg
     2401 ccgtgagcga gctgctgcgc gacacgcgcg cgcgccaggg tcggctgcgc gaggagcgcg
     2461 aacgcgcgcg cgactcgctc aagggcctga tcaaccggat gctggcagag ctcgacgagc
     2521 tcggctcgca gaccggcaac ttccacgaca gcgtcggccg ctatgccgac gtgatcgaga
     2581 aggccgattc gcttgagagc ctgaccggcg tcgtgcgcga gatggtcgaa gaaagccgca
     2641 ccgtgcaggc gctggtgcag cagacccagg cgcggctgca ggccgagcat ggcaaggcgc
     2701 aggggctgac gcagcgcgtc gtcgagctcg aagcggagat gcagcgcctg tcgggcgagg
     2761 tctcgaccga ccagctgacc cagatcgcga accgccgcgg cctgctgctg gccttcgacg
     2821 ccgagtgcgc gcggctggcg cgttccggca gcacgctcag cgtcggcctg ctcgacatcg
     2881 acaacttcaa gcgcctgaac gacgaactcg gccacggcgc gggcgacgag gcgctgaagt
     2941 cgctcgccgc ggtcgtcagc aagaccttgc ggccgaccga ccacgtggcg cgctacggcg
     3001 gcgaggagtt cgtcgtgctg ttgcccgaca cgccggtcat cgaggcccgg cagatcctga
     3061 cccggctgca gcgttcgctg accggcggcc tgttcatgca cgagcacaag caggtgttcg
     3121 tgaccttctc ggccggcgtc acggcccacc ggccgggcga gcgcatcgag caggcgctgg
     3181 agcgcgccga ccagggcctg tacgaagcca agcgcaccgg caagaaccgg acttgtctgg
     3241 gttagtgggg gcggcttcag caacccttca ggctcgccgc gccggcgccg gccgccgcaa
     3301 gcaccgcgcc caccgcttcg cgatcgctca gccgctgctg tttcgcgagc gcgccggcct
     3361 gcgcggcctg cgtttccagc gtcgcgcggc tcgaccggcc gctgcacgcg agcaggtaca
     3421 ccacatggcg cgaggtgccg agcgtggcgc cggtgttcga cgcgatgcgc ggcgccatcg
     3481 ccatcaggtc gtcgtagctc atgccgacgc gcggcgcgac ctcgctcagg tgcttggtct
     3541 cgctgcggcc gacgaagaag acgaccacga aggccaccgc cagcccgagc ccgacgatcg
     3601 tcgcgatgta cctgaccatg cctgctgcgc tcctcggcgt gccgggtcgt ccttgatgat
     3661 cttccacttg acctcgtcga ggtacggaag ccggttgccc tgcttgtcct tcagccagaa
     3721 gtacgagttc cgggagaggt cgatctccgc ccctctctgc cacgacttca ggcagaacgg
     3781 gccgcttccc agcggcttgc tcgccaggcc cttgtcgccc accttcgccg cgtacctctg
     3841 cgagacgacc gccgtccccc agagcgtgag gtaagagagg aagctggcgt tcgccgtcgg
     3901 gagtgtgatc accaccgtgc ccgggccgcg cgccgtgatc ttcatcccct tggggtactg
     3961 ccagctgacg atcgccttcg gcgtgctcgc ccgcttgaac gagaagacga cgtcctgcgc
//
LOCUS       DASRDG010000396         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_61907, whole genome shotgun sequence.
ACCESSION   DASRDG010000396 DASRDG010000000
VERSION     DASRDG010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61907"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..971
                     /locus_tag="VFA35_06550"
     CDS             33..971
                     /locus_tag="VFA35_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZT55866.1"
                     /translation="MSDRAPPVPEPARDAAPAGAASHLEFKATLLLIVLVLLVGGAAL
                     YLSWARGAFERTQQLVLVAEDAEGVGVGMDLTFAGFPIGRVRRIELAPDGNARIVVDV
                     PVKDAHWLRETSVFAIQRGVVGNTNIRAYTGVLSDPPLPDGAVRTVLRGDAAGEIPRL
                     MAEARQLLSNLNAISAEGSAVNESLTNLRGFTAKLNATGGALDRTNTLLARLDGLAAK
                     ADTQVFGSDGMLRDARQTVQQLNNVLGDARASLQRVDAVLEEARAVGANARVATTDLG
                     ALRADVETSLRNIDALIQEVNRKWPFAKKDAEIKLP"
     gene            968..1612
                     /locus_tag="VFA35_06555"
     CDS             968..1612
                     /locus_tag="VFA35_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55867.1"
                     /translation="MKFLYALFIVVLLTACSNNPPVPDWQVNAKTATERAVAAYLVGN
                     ARVDAAELARARAEVARSGRADLLARVELAHCAAQVASLAFGPCAGFEPLRADAGAPE
                     RAYADYLLAKAAPADTALLPEAHRGVAPASIPDPLARLVAAGVLFEAGRADPALIALA
                     VDTASAQGWRRPLLAWLGVQLRRAEQAGAPDEAERLRRRIALVQGGAAAPATSP"
     gene            complement(1599..>2587)
                     /locus_tag="VFA35_06560"
     CDS             complement(1599..>2587)
                     /locus_tag="VFA35_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HZT55868.1"
                     /translation="AAARGATTLENCRVRDVAFDADGATVQAELTGADGQPVPKQWRA
                     RFVIDASGRDTLLGNKLKIKQKNPKHNSSALFGHFTNAERLPGKKEGNISICWFAHGW
                     FWFIPLADGTTSVGAVCWPYYLKSRRKPLREFFFDTIALCPELARRLKSATLVDDAVH
                     ATGNYSYLATHATGERYLMLGDAYTFIDPMFSTGVYLAMHSAFEGAELVATALDRPAE
                     FAAQRAAFEAMMKKGPKEYSWFIFRVTNPTIRELFMHPANPFRVLEALMSMLAGDIYG
                     KTPLWGPLRILKGIYYLISLRHLRRTVGGWLRHRRNIRDMGMLDGETILKAK"
BASE COUNT          362 a          920 c          931 g          374 t
ORIGIN      
        1 tctcgctggt gggcaactac tactgacgcg cgatgagcga ccgtgcgcca cccgttccgg
       61 aacccgcgcg tgacgccgcg cccgcgggcg cggcgagcca cctcgagttc aaggccacgc
      121 tgctgctgat cgtgctcgtg ctgctggtcg gcggcgcggc gctgtacctg agctgggcgc
      181 gcggcgcgtt cgagcgcacc cagcagctgg tgctcgtggc cgaagacgcc gaaggcgtcg
      241 gcgtcggcat ggacctgacc ttcgccggct tccccatcgg ccgggtgcgg cgcatcgagc
      301 tcgcgcccga cggcaacgcg cgcatcgttg tcgacgtgcc ggtgaaggac gcgcactggc
      361 tgcgcgaaac cagcgtgttc gcgatccagc gcggcgtcgt cggcaacacc aacatccgcg
      421 cctacaccgg cgtgctgagc gacccgccgc tgcccgacgg cgcggtgcgc accgtgctgc
      481 gcggcgacgc cgcgggcgag atcccgcggc tgatggccga ggcgcgccag ctgctgtcga
      541 acctgaacgc gatcagtgcc gagggctcgg ccgtcaacga gagcttgacg aacctgcgcg
      601 gcttcaccgc gaagctgaac gcgaccggcg gcgcgctgga ccggaccaac acgctgctgg
      661 cgcggctcga cggcctggcc gcgaaggccg acacgcaggt cttcggcagc gacggcatgc
      721 tgcgcgacgc gcgccagacc gtgcagcaac tgaacaatgt gctcggcgat gcgcgcgcga
      781 gccttcagcg cgtcgacgcg gtgctcgaag aggcgagggc cgtcggcgcg aacgcgcgcg
      841 tcgcgaccac cgacctgggc gcgctgcgcg ccgacgtcga gaccagcctg cgcaacatcg
      901 acgcgctgat ccaggaagtg aaccggaagt ggccgttcgc gaagaaggac gcggagatca
      961 agcttccatg aaattcctct acgcgttgtt catcgtcgtg ctactgaccg cctgctcgaa
     1021 caacccgccc gtgcccgact ggcaggtcaa cgcgaagacg gcgaccgagc gcgcggttgc
     1081 cgcctacctg gtcggcaacg cgcgcgtcga cgcggccgaa ctcgcgcggg cgcgcgcaga
     1141 ggtcgcgcgc agcggccgcg ccgatctgct cgcgcgcgtc gagctcgcgc actgcgcggc
     1201 gcaggtcgcg agcctggcgt tcggcccgtg cgccggcttc gagccgctgc gcgccgatgc
     1261 cggcgcgccg gaacgcgcct atgccgacta cctgctggcg aaggccgcgc ctgccgatac
     1321 ggcgctgctg cccgaagcgc accgcggcgt cgcgcctgct tcgatccccg acccgctcgc
     1381 gcggctggtc gctgccggcg tgctgttcga ggccggtcgc gccgacccgg cgctgatcgc
     1441 gctcgcggtc gacaccgcct cggcgcaggg ctggcgccgc ccgctgctcg cgtggctggg
     1501 cgtgcaactg cggcgcgccg agcaggccgg cgcgcccgac gaggccgagc gcctgcgccg
     1561 ccgcatcgcg ctggttcagg gcggcgcggc cgcgccggct acttcgcctt gaggatcgtc
     1621 tcgccgtcga gcatccccat gtcgcggatg ttccggcggt gccgcagcca gccgccgacc
     1681 gtgcgccgca ggtggcgcag cgagatcagg tagtagatgc ccttcaggat gcgcagcggc
     1741 ccccacagtg gcgtcttgcc gtagatgtcg ccggcgagca tcgacatcag cgcctccagc
     1801 acccggaacg ggttcgccgg gtgcatgaac agctcgcgga tcgtcgggtt cgtgacgcgg
     1861 aagatgaacc acgaatactc cttcgggccc ttcttcatca tcgcctcgaa cgcggcccgc
     1921 tgcgccgcga actcggccgg ccggtcgagc gcggtcgcga ccagttcggc gccctcgaac
     1981 gcgctgtgca tcgcgaggta gacgccggtc gagaacatcg ggtcgatgaa ggtgtaggcg
     2041 tcgccgagca tcaggtagcg ctcgccggtg gcgtgcgtcg ccaggtacga gtagttgccg
     2101 gtcgcgtgca ccgcgtcgtc gaccagcgtc gcgctcttca ggcggcgcgc gagctcgggg
     2161 cacagcgcga tcgtgtcgaa gaagaattcg cgcaagggct tgcggcgcga cttcaggtag
     2221 tacggccagc acaccgcgcc gacgctggtc gtgccgtcgg ccagcgggat gaaccagaac
     2281 cagccgtgcg cgaaccagca gatgctgatg ttgccttcct tcttgccagg caggcgctcg
     2341 gcgttggtga agtggccgaa cagcgccgag ctgttgtgct tcgggttctt ctgcttgatc
     2401 ttcagcttgt tgccgagcag cgtgtcgcgc cccgacgcgt cgatcacgaa gcgcgcgcgc
     2461 cactgcttcg gcacgggctg gccgtcggcg ccggtgagct cggcctgcac ggtcgcgccg
     2521 tcggcgtcga aggcgacgtc gcgcacgcgg cagttctcga gcgtggtcgc gccgcgcgcc
     2581 gcggcgt
//
LOCUS       DASRDG010000397         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_632465, whole genome shotgun sequence.
ACCESSION   DASRDG010000397 DASRDG010000000
VERSION     DASRDG010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632465"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..962)
                     /gene="egtD"
                     /locus_tag="VFA35_06565"
     CDS             complement(39..962)
                     /gene="egtD"
                     /locus_tag="VFA35_06565"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZT55869.1"
                     /translation="MADAAAERRAIEAGLLQRPAHASPKYFYDRLGSHLFEAITELPE
                     YYPTRTEAAIFATHGAAMARRIGAGATLIDLGAGNCAKAARLFPLLEPRRYVAVDISA
                     DFLRDALKRVQREHPMLEVVGLGQDFSAALELDDDLVAGRGVFFYPGSSIGNFTPDEA
                     LAFLARVRAQARAGGLLIGVDLVKPRDVLESAYDDALGVTAAFNRNALVHLNRLIGSD
                     FVPRQWRHVAFFDDVKSRIEMHLEASEDVEVSWPGGGRRFAAGERIHTENSCKYTASN
                     FADLLVGAGFDAPRRWTDPAGWFAVFWAQAR"
     gene            1167..1646
                     /locus_tag="VFA35_06570"
     CDS             1167..1646
                     /locus_tag="VFA35_06570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZT55870.1"
                     /translation="MARAAHMVRPWPATKPTPALALTDLDGKAWSLAMLNGRPVLLNF
                     WASWCEPCRTEMPSLELLQTRHEKAGLVILSVNYQEPLPQIRRFVDALPFSLPVLIDR
                     EGEATGAWTPRVFPSTVLIDRGGTPRSTVIGELDWMGDTARALVAPLLRPLPSARSA"
     gene            1710..>2060
                     /locus_tag="VFA35_06575"
     CDS             1710..>2060
                     /locus_tag="VFA35_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HZT55871.1"
                     /translation="MSMNTNRLKRREWLALTTTALAAAAWPGRAAAQARRFDPQPGDW
                     RRFEITTQVEIANPNGASRVWLPMPSIDTAYQQSLDSQWAGNATRVKVAADTHYGAKM
                     LAAEFDAGVARPTVQ"
BASE COUNT          308 a          724 c          738 g          290 t
ORIGIN      
        1 tcctcgccga gcactgacgc agcgccgggc gcgctgcctc agcgcgcctg cgcccagaac
       61 accgcgaacc agccggccgg atcggtccag cgccgcggcg cgtcgaagcc ggcaccgacc
      121 agcagatccg cgaagttgga cgccgtgtac ttgcacgagt tctcggtgtg gatgcgctcg
      181 ccggcggcga agcggcgccc gccgccgggc cacgagacct cgacgtcttc gctcgcttcg
      241 aggtgcatct cgatgcgcga cttcacatcg tcgaagaagg cgacgtggcg ccattggcgc
      301 ggcacgaaat cgctgccgat caggcggttc aggtggacca gcgcattgcg attgaaggcc
      361 gccgtgacgc cgagcgcgtc gtcgtaggct gactcgagga cgtcgcgcgg cttgaccagg
      421 tcgacgccga tcagcaggcc gccggcgcgc gcctgcgcgc gcacgcgcgc gaggaatgcg
      481 agcgcttcgt cgggcgtgaa gttgccgatg ctcgagccgg ggtagaagaa cacgccgcgg
      541 ccggcgacca agtcgtcgtc gagctccagc gcggccgaga aatcctgccc gagaccgacc
      601 acctcgagca tcggatgctc gcgctgcacg cgcttcagcg cgtcgcgcag gaagtcggcc
      661 gagatgtcga ccgcgacata ccggcgcggc tccagcagag ggaacagccg ggccgctttc
      721 gcgcagttgc ccgcccccag atcgatcagc gtcgcgccgg cgccgatgcg ccgcgccatg
      781 gccgcgccgt gggtcgcgaa gatggctgcc tcggtgcgcg tcgggtagta ctcgggcagt
      841 tcggtgatcg cctcgaacag gtgcgagccg aggcggtcgt agaagtactt cggcgacgca
      901 tgggcgggcc gctgcaacag gccggcctcg atcgcgcggc gctcggctgc ggcgtcggcc
      961 acgtggcgct cgatgaatcg gggcgtgcgc aatggggtca tggccggatg tggtgcaatc
     1021 gtgccgacat ggcccgaggg tgtcgataga cggcgcccaa ccttacaccc accggattca
     1081 ggatgcagcg cagaaatttg cctcgcccgg gtaggacgac gcgccgtcgg ctgctggccg
     1141 gcgcgctcgc gttggcgttg ccgggcttgg cgcgcgccgc gcacatggtg cggccgtggc
     1201 cggcgaccaa gccgacgccc gcgctcgcgc tgaccgatct cgacggcaag gcctggtcgc
     1261 tcgcgatgct caacggccgg ccggtgttgc tgaacttctg ggccagctgg tgcgagccgt
     1321 gccgcaccga gatgccgtcg ctcgaactgc tgcagacgcg ccacgagaag gccgggctgg
     1381 tgatcctgtc ggtcaactac caggagccgc tgccgcagat caggcgcttc gtcgacgcgc
     1441 tgccgttctc gctgccggtg ctgatcgacc gcgaaggcga ggccaccggg gcgtggaccc
     1501 cgcgcgtgtt cccgagcacc gtgctgatcg accgcggcgg tacgccccgc agcaccgtga
     1561 tcggcgagct cgactggatg ggcgacacgg cacgcgcgct ggttgcgccg ctgctgcggc
     1621 ccttgccctc ggcccgatcc gcctgagccg gcgtggcggg caccgataat ccctcaccct
     1681 tttttcccgt tcccgatgga gtcgaccgca tgagcatgaa cacgaaccgc ctgaaacgcc
     1741 gcgagtggct ggcgctgacg acgaccgcgc tggccgccgc cgcatggccc ggtcgcgccg
     1801 ctgcgcaggc ccgccgcttc gacccgcagc cgggcgactg gcgtcgcttc gagatcacga
     1861 cccaggtcga gatcgcaaac ccgaacggcg cctcgcgcgt gtggctgccg atgccgtcga
     1921 tcgacaccgc ctaccagcag tcgctggaca gccagtgggc cggcaacgcg acgcgcgtga
     1981 aggtcgcggc cgacacgcac tacggcgcga agatgctcgc ggccgagttc gacgccggcg
     2041 tggcgcggcc gacggtgcag
//
LOCUS       DASRDG010000398         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1212790, whole genome shotgun sequence.
ACCESSION   DASRDG010000398 DASRDG010000000
VERSION     DASRDG010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212790"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFA35_06580"
     CDS             <1..782
                     /locus_tag="VFA35_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HZT55872.1"
                     /translation="GLAQLHQLRGRVGRSHHQAYAYLLTPPEEALSLQAKKRLEAIQM
                     MEDLGAGFFLAMHDLEIRGAGEVLGDNQSGNMMEVGFQLYNEMLAEAVRSLKMGVEPD
                     LLSPLSATTEINLHAPALLPDAYCGDVHTRLSLYKRLASAGTSAQIDALLEEVTDRFG
                     KLPPQGQTLFDVHRLRVLAKPFGVTKIDAGAKLMVISFRPNPPVEALRIIELVQKNRH
                     IRLAGNDKLRIERDTPEPKDRAQLIRDVLRALGAPKQALAA"
     gene            851..1222
                     /locus_tag="VFA35_06585"
     CDS             851..1222
                     /locus_tag="VFA35_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55873.1"
                     /translation="MKTTFDLPEPLLRRAKAAAAHQGRPLRDLVAEAIDAKLAADRAA
                     THAASKQPSEWEAFKAQLVQQPDGTWLNPNGIEDEGFFEALDGIRHERLMQQAPREPI
                     AELPTRSPRRKPAAARRRKPA"
     gene            1250..1660
                     /locus_tag="VFA35_06590"
     CDS             1250..1660
                     /locus_tag="VFA35_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZT55874.1"
                     /translation="MFLIDTNVLSEVLRPRPNLSVARLLHTTAPELRFASEITRYELR
                     MGARLRADADAFWARIEQRILPLVQWLPIGAAVALAGADLAASLRRAGQPLEWTDLMV
                     GATAQVHGLTMVTRNVRHFERVPGIAVENWFATA"
     gene            1704..2417
                     /gene="serB"
                     /locus_tag="VFA35_06595"
     CDS             1704..2417
                     /gene="serB"
                     /locus_tag="VFA35_06595"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HZT55875.1"
                     /translation="MTARQFAPGLFVRGFTPPLRLADYKLVAFDMDSTLINIECVDEI
                     AAAAGRKAEVAAITEAAMRGEITDYKESLRRRLALLRGVPEAALHAVYANHLQLNPGV
                     ERFVQACQAAGLKTLLVSGGFTFFSERVRARLGLDFARANMLEIEHGALTGRLIDRPW
                     GDIVDGAEKRRVVLEVCELMGIAPAQAIAVGDGANDLPMMGAVGLSIAYHPKPAVLEQ
                     AMLSITRGGMERALELFGV"
BASE COUNT          436 a          943 c          895 g          392 t
ORIGIN      
        1 tcggtcttgc gcagttgcat cagctgcgcg gtcgcgtcgg ccgttcgcac caccaggcgt
       61 acgcgtatct gctgacgccg ccggaggaag cgttgtcctt gcaggcgaaa aagcgcctcg
      121 aagcgatcca gatgatggag gatctcggcg cgggattctt cctcgcgatg cacgacctcg
      181 agatccgcgg cgccggcgag gtgctcggcg acaaccagag cggcaacatg atggaggtcg
      241 gcttccagct ctacaacgag atgctggccg aggcggtgcg ctcgctgaag atgggcgtcg
      301 agcccgacct gctctcgccg ctgtcggcca ccaccgagat caacctgcac gcgcccgcgc
      361 tgctgcccga tgcctactgc ggcgacgtgc acacgcgcct gtcgctgtac aagcggctcg
      421 ccagcgccgg cacttcggcg cagatcgacg cgctgctcga agaagtcacc gaccgcttcg
      481 gcaagctccc gccgcagggc cagacgctgt tcgacgtgca ccgcctgcgc gtgctcgcca
      541 agccgttcgg cgtgaccaag atcgacgccg gcgcgaagct gatggtgatc agcttcaggc
      601 cgaacccgcc ggtcgaggcc ctgcgcatca tcgagctggt gcagaagaac cgccacatcc
      661 gattggccgg caacgacaag ctgcgcatcg agcgcgacac gcccgagccg aaggaccgcg
      721 cgcagctcat ccgcgacgtg ctgcgcgcgc tcggcgcgcc aaaacaagca ctggcggcct
      781 gacccatgga caccagcctg tggccgactc cgacttgacg aacaccccct gaaccgtaaa
      841 tactgccagg atgaaaacca ccttcgattt gccggaaccg ctgttgcgcc gtgccaaggc
      901 tgctgccgcc caccagggcc ggcccttgcg cgacctggtg gccgaggcga tcgacgccaa
      961 gctcgccgcc gaccgggccg cgacgcacgc cgcatcgaag caaccgagtg aatgggaggc
     1021 cttcaaggcg cagctcgtcc agcaacccga cggcacctgg ctcaatccca acggcatcga
     1081 ggatgaaggc tttttcgaag cgctcgacgg aatccgtcac gagcgcctca tgcagcaagc
     1141 gccgcgcgag cccatcgcgg agctgccgac ccgctcgccg cgccgcaagc ctgcggcagc
     1201 ccgtcggcgc aagcccgctt gatcgatatt tgcccgagct cgcgcgccct tgttcctgat
     1261 cgataccaac gttctcagcg aggtgctgcg cccgcggccg aacctgagcg tcgcgcgctt
     1321 gctgcacacc accgcgcccg agttgcgttt cgcgtccgag atcacccgct acgaactgcg
     1381 catgggtgcg cgcctgcgcg ccgacgccga cgcgttctgg gcgcgcatcg agcagcgcat
     1441 cttgccgctc gtgcagtggt tgccgatcgg tgccgccgtg gcgctggccg gtgccgacct
     1501 cgcggcctcc ctgcgccgtg ccggacaacc gctggaatgg accgatctga tggtcggcgc
     1561 gacggcacaa gtgcacggcc tgacgatggt gacgcgcaac gtccgccatt tcgaacgcgt
     1621 gccgggcatc gccgtcgaga actggtttgc gacggcgtag cgcatgagcc ggcgcagcgc
     1681 atgaggaacc tggggtaacg cagatgaccg ctcggcagtt cgcccccggc ctgttcgttc
     1741 gcggcttcac gccgccgctg cggctggccg actacaagct ggtcgcgttc gacatggact
     1801 cgacgctgat caacatcgag tgcgtcgacg agatcgccgc cgcggccggc cgcaaggccg
     1861 aggtggccgc gatcaccgag gccgcgatgc gcggcgagat caccgactac aaggagagcc
     1921 tgcgccgtcg cctcgcgctg ctgcgcggcg tgcccgaggc ggcgctgcac gcggtgtacg
     1981 cgaaccacct gcagctgaac cccggcgtcg agcgcttcgt gcaggcctgc caagcggccg
     2041 ggctgaagac gctgctggtc tcgggcggct tcacgttctt cagcgaacgg gtgcgcgcgc
     2101 ggctcggcct ggacttcgca cgcgccaaca tgctcgagat cgagcacggc gccctcaccg
     2161 gccggctgat cgaccggccg tggggcgaca tcgtcgacgg cgccgagaag cggcgcgtcg
     2221 tgctcgaggt ctgcgaactg atgggcatcg cgccggcgca ggcgatcgcg gtcggcgacg
     2281 gcgcgaacga cctgccgatg atgggcgcgg tcggcctgag catcgcctac cacccgaagc
     2341 cggcggtgct ggaacaggcg atgctctcga tcacccgcgg cggcatggag cgcgcgctcg
     2401 aactgttcgg cgtttgaata gaatcgaggg tggcggccgc tcttgtggag ccgcgccctg
     2461 gcgagcgcgg cacgtgccgt caggccaggt ttttcgttcc gcccaaggag gcctcgtgca
     2521 gcgcaagctc gccgcgattc tgagcgccga cgccgccggc tacagccggc tgatgggcga
     2581 cgacgaactg gccacggtgc gcacgctgtc ggcctgccag aagctcgtct gcgacgcgat
     2641 cgcagcgcac cgcggccgca ccatcg
//
LOCUS       DASRDG010000399         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_292177, whole genome shotgun sequence.
ACCESSION   DASRDG010000399 DASRDG010000000
VERSION     DASRDG010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292177"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /gene="mrdA"
                     /locus_tag="VFA35_06600"
     CDS             <1..1839
                     /gene="mrdA"
                     /locus_tag="VFA35_06600"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZT55876.1"
                     /translation="LILDRNGVVLANNYSAYTLEITPGKLEASLDSTIDKLAEIVDIQ
                     PRDRKRFKRLMDENKSFESLPIRTKLSDDEVARFTAQRFLFAGVDIKARLFRNYPLGQ
                     VGSHLIGYIGRINEAEKEKIDDSEDEANYRGTQYIGKLGVEQSYEAELHGTTGFEEME
                     TSAGGHPVRRLKSNPATPGNALVMSIDIRLQAMVEELFGDRRGALVAIDPRNGEVLAF
                     VSKPNFDPNLFVDGIDQENWKALNESLDKPLLNRALRGTYSPGSTYKPFMALAALTLG
                     KRTPQQLTYDPGYFNFGGHTFRDDKEGGHGSVDMYKSIVQSCDTYYYALANDLGVDAM
                     HDFMAPLGLGQLTGIDMQGELRGVLPSTAWKRNAYKKKELQKWYAGETISLGIGQGQN
                     TFTMLQLAQATAALASGGHRYKPHLVKVIENFETRTKTRTIEQALPTLDWKPEHVAVI
                     RHAMFGVTQEGTSRSSFVGASYQSGGKTGTAQVIGIKQGEKYNAKNVDERHRDHALYT
                     AFAPLDEPRIALAMVVENAGFGAQNAAPIARRVFDLVLTGQYPSEADIAATRLGQSTA
                     PIGTPRPIASVPLPGVTVDGGAAAAPAAASAAAGSPTQRVALGTSR"
     gene            1836..2993
                     /gene="rodA"
                     /locus_tag="VFA35_06605"
     CDS             1836..2993
                     /gene="rodA"
                     /locus_tag="VFA35_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZT55877.1"
                     /translation="MSAVFDRPSPWQRIKPVFSGFDIPLLLAILLLGVAGMLTMYSAG
                     FDAGTRFVDHGRNMLLALCVIFVVAQVSPQTLMRFAVPLYALGVALLVAVAVMGVTKK
                     GATRWLNVGVVIQPSEILKIAMPLMLAWWFQRREGRLRVPDFVIAGLLLVVPVGLIVK
                     QPDLGTAILVLSAGFYVIFFAGLSWKLIVPVLVVGAIGITAVVVSEDRICQPEVSWLP
                     VLKDYQKNRVCTLLDPTTDPLGKGFHIIQGMIAIGSGGVEGKGFMKGTQTHLEFIPER
                     TTDFIFAAYSEEFGLIGCGALLLGFIFLIFRGLMIAADAPTVFTRLLAGAITLSWFTY
                     AFVNMGMVSGILPVVGVPLPFISYGGTAMVTLGLGLGILMSISKSRRLIQS"
     gene            complement(2926..>3356)
                     /locus_tag="VFA35_06610"
     CDS             complement(2926..>3356)
                     /locus_tag="VFA35_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT55878.1"
                     /translation="PQGPQVLGHALLLRELIRNLVDNALQYTPGGGTVTVRVIDDPFG
                     QVVVLQVEDSGPGIAPTERELVFQPFYRSLGTEVDGSGLGLAIVREIALLHGAEVTVA
                     DASSRSPPGTLVTLRLPALSSESAADSSRSTSGSPAPGPA"
BASE COUNT          549 a         1147 c         1139 g          521 t
ORIGIN      
        1 ctgatcctcg atcgcaacgg cgtcgtgctc gcgaacaact attcggccta cacgctcgag
       61 atcacgccgg gcaagctcga ggcgagcctc gattcgacga tcgacaagct cgccgagatc
      121 gtcgacatcc agccgcgcga ccgcaaacgc ttcaagcggc tgatggacga gaacaagagc
      181 ttcgagtcgc tgccgatccg caccaagctc agcgacgacg aggtcgcgcg cttcaccgcg
      241 cagcgcttcc tgttcgccgg tgtcgacatc aaggcgcggc tgttccgcaa ctacccgctc
      301 ggccaggtcg gcagccacct gatcggctac atcggccgca tcaacgaggc cgaaaaggag
      361 aagatcgacg actcggaaga cgaagccaac taccgcggca cgcagtacat cggcaagctc
      421 ggcgtcgagc agagctacga ggccgagctg cacggcacga cgggcttcga ggagatggag
      481 acgagcgccg gcggccaccc ggtgcgccgc ctgaagagca acccggcgac gcccggcaac
      541 gcgctcgtga tgtcgatcga catccggctg caagcgatgg tcgaggagct gttcggcgac
      601 cgccgcggcg cgctcgtcgc gatcgacccg cgcaacggcg aggtgctcgc gttcgtcagc
      661 aagccgaact tcgacccgaa cctgttcgtc gacggcatcg accaggagaa ctggaaggcg
      721 ctgaacgagt cgctcgacaa gccgctgctg aaccgcgcgc tgcgcggcac gtattcgccc
      781 ggctcgacct acaagccctt catggcgctg gcggcgctga cgctgggcaa gcgcacgccg
      841 cagcagctca cctacgaccc gggctacttc aacttcggcg gccacacctt ccgcgacgac
      901 aaggagggcg ggcacggctc ggtcgacatg tacaagtcga tcgtgcagtc gtgcgatacc
      961 tactactacg cgctcgcgaa cgacctcggc gtcgacgcga tgcacgactt catggcgccg
     1021 ctcggcctcg ggcagctcac cggcatcgac atgcagggtg agctgcgcgg cgtgctgccc
     1081 tcgaccgcgt ggaagcgcaa cgcctacaag aagaaggagc tgcagaagtg gtacgccggc
     1141 gagacgatct cgctgggcat cggtcagggc cagaacacct tcacgatgct gcagctcgcg
     1201 caggcgacgg cggcgctggc ctccggcggc catcgctaca agccccacct ggtgaaggtg
     1261 atcgagaact tcgagacccg caccaagacg cgcacgatcg agcaggcgtt gccgaccctc
     1321 gactggaagc ccgagcacgt tgccgtgatc cggcacgcga tgttcggcgt gacgcaggaa
     1381 ggcacgtcgc gctcgtcgtt cgtcggcgcg agctaccagt cgggcggcaa gaccggcacc
     1441 gcgcaggtca tcggcatcaa gcaaggcgag aagtacaacg ccaagaacgt cgacgaacgc
     1501 caccgcgacc atgcgctgta caccgcgttc gcgccgctcg acgagccgcg catcgcgctc
     1561 gcgatggtgg tcgagaacgc cggcttcggg gcccagaatg ccgcgccgat cgcgcgccgc
     1621 gtgttcgacc tcgtgctcac cgggcagtac ccgagcgagg ccgacatcgc cgccacccgg
     1681 ctcggccagt cgaccgcgcc gatcggcacg ccgcgcccga tcgcctcggt gccgctgccc
     1741 ggcgtgacgg tcgacggcgg ggccgcggcg gcgcccgccg cggcgagcgc agcggccggg
     1801 tcgccgacgc agcgcgtcgc gctgggcaca tcgcgatgag cgccgtgttt gaccgcccgt
     1861 cgccgtggca gcgcatcaag ccggtgttca gcggcttcga catcccgctg ctgctggcga
     1921 tcctgctgct cggcgtggcc ggcatgctga cgatgtactc ggctggcttc gacgcgggca
     1981 cgcgcttcgt cgaccacggc cgcaacatgc tgctggcgct ttgcgtgatc ttcgtcgtcg
     2041 cgcaggtctc gccgcagacg ctgatgcgct tcgcggtgcc gctgtatgcg ctcggcgtcg
     2101 cgctgctggt cgcggtggcg gtgatgggcg tcacgaagaa gggcgcgacg cgctggctca
     2161 acgtcggcgt cgtgatccag ccgagcgaga ttctcaagat cgcgatgccg ctgatgctgg
     2221 cctggtggtt ccagcgccgc gagggccggc tgcgcgtgcc cgacttcgtc atcgcggggc
     2281 tgctgctggt ggtgccggtc ggcctgatcg tcaagcagcc cgacctcggc accgcgatcc
     2341 tggtgctgtc ggccggcttc tacgtgatct tcttcgccgg gttgagctgg aagctgatcg
     2401 ttccggtgct cgtcgtcggc gcgatcggca tcaccgcggt cgtcgtctcg gaagaccgca
     2461 tctgccagcc cgaggtgagc tggctgccgg tgctcaagga ctaccagaag aaccgcgttt
     2521 gcacgctgct cgacccgacc accgacccgc tcggcaaggg cttccacatc atccagggga
     2581 tgatcgcgat cggctccggc ggcgtcgagg gcaagggctt catgaagggc acgcagaccc
     2641 acctcgagtt cattcccgag cgcaccaccg acttcatctt cgccgcctac tccgaagagt
     2701 tcggcctgat cggctgcggc gcgttgctgc tcggcttcat cttcctgatc tttcgcgggc
     2761 tgatgatcgc ggccgacgcg ccgaccgtgt tcacgcggct gctcgcgggg gcgatcacgc
     2821 tgtcgtggtt cacctacgcg ttcgtcaaca tgggcatggt cagcggcatc ctgccggtgg
     2881 tcggcgtgcc gctgccgttc atcagctacg gcggcaccgc gatggtcacg ctgggcctgg
     2941 ggctggggat cctgatgtcg atctcgaaga gtcggcggct gattcagagc tgagtgccgg
     3001 tagccgcagc gtgaccaggg tgcccggcgg gctgcggctc gacgcatcgg cgaccgtgac
     3061 ttcggcgccg tgcaggagcg cgatctcgcg cacgatcgcc aggccgaggc cggagccgtc
     3121 gacctcggtg ccgagcgagc ggtaaaaggg ctggaacacc agctcgcgtt cggtcggcgc
     3181 gatgccgggg ccggagtcct cgacctgcag cactacgacc tggccgaacg gatcgtcgat
     3241 cacgcgcacc gtgaccgtgc cgccgcccgg cgtgtactgc aaggcgttgt cgaccaggtt
     3301 gcggatcagc tcgcgcagca gcagcgcgtg gccgagcacc tgcgggcctt gcgggt
//
LOCUS       DASRDG010000400         5941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_421620, whole genome shotgun sequence.
ACCESSION   DASRDG010000400 DASRDG010000000
VERSION     DASRDG010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421620"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..933)
                     /locus_tag="VFA35_06615"
     CDS             complement(49..933)
                     /locus_tag="VFA35_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZT55879.1"
                     /translation="MEIFGIPIQAFLGQLLLGLVNGSFYAMLSLGLAVIFGLLGIVNF
                     AHGALYMIGAYVAFVALDKFGINYWFALIVSPLVVGALGVVIERTLLKRLYKIDPIYG
                     LLLTFGLALIAEGLFRYWFDVSGQSYPVPDLLQGATNLGFMVLPNYRAWVVVASLAVC
                     LGTWYLIERTRLGAYLRAGTENPTLVQAFGINVPVMVMLTYGGGCALAAFAGVLAAPV
                     IQITPLMGSNLIIVVFAVVVIGGMGSILGSILTGLALGLIEGLTKVFYPEASSIVVFV
                     IMAIVLMVRPAGLFGKEA"
     gene            complement(952..2160)
                     /locus_tag="VFA35_06620"
     CDS             complement(952..2160)
                     /locus_tag="VFA35_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT55880.1"
                     /translation="MKLTHLSAACALAATTLFGASAQAQISGDTIKIGFISDLSGLYA
                     DIDGPAGGEAIRMAVADLGGTVAGKKIEVLVADHQNKPDVAASKAREWFDQQGLDVLI
                     GGTNSGTSLAMAKVAAEKKKPFLAVGAGSSALTNDQCSPYTIHYAYDTTALAKGTAPA
                     VVKAGGKSWYFLTADYAFGTALQNDATNAVKAAGGSIVGSVRHPLSASDFSSFLLQAQ
                     SSKAQILGLANAGGDTINAVKAANEFGVTKTMKLAGLLMFINDVHSLGLKATQGMYLT
                     DSWYWNQSAESRAWSKRFFDKFKRMPSSLQAADYSVAQFYLNAVKAAGTDDGEKVMAK
                     MRESKVNDMYAKGGYVRADGRMVHDMFLMQVKTPAQSTTPWDYYNVVATTKGEDAFTT
                     KAESKCALWK"
     gene            complement(2205..2909)
                     /locus_tag="VFA35_06625"
     CDS             complement(2205..2909)
                     /locus_tag="VFA35_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT55881.1"
                     /translation="MATPFLEVKDLQGWYGESHVLHGVNFRVDEGEVVTLLGRNGAGR
                     TSTMRAIMGLIGTRKGSIKVRGTETIAMATHRIARLGVGYCPEERGIFSSLSAEENLM
                     LPPTLAEGGMSVEQIYTMFPNLKERASSQGTRLSGGEQQMLAVARILRTGARLLLLDE
                     ISEGLAPVIVQKLAEMVIALRQQGYTVVMVEQNFRFAAPLADRFMVMEHGQVIQEFTQ
                     AELPSRLERLHEYLGV"
     gene            complement(2911..3684)
                     /locus_tag="VFA35_06630"
     CDS             complement(2911..3684)
                     /locus_tag="VFA35_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT55882.1"
                     /translation="MSGEFILETRQLTKEFKGFVAVSKVDLKVRRGSIHALIGPNGAG
                     KTTCFNLLTKFLIPTSGQIVFDGTDITPEKPAAIARRGVIRSFQISAVFPHLSVLENV
                     RVALQRKLGTSYQFWKPERSLETLDAQALALLREVDLEAYARIPAVELSYGRKRALEI
                     ATTLAMDPKLMLLDEPTQGMGLEDVDRIRQLIKKVAASRTVLMVEHNMSVVSSIADTI
                     TVLQRGATLAEGPYEQVSKNPAVIEAYMGSANVELKGAH"
     gene            complement(3681..4514)
                     /locus_tag="VFA35_06635"
     CDS             complement(3681..4514)
                     /locus_tag="VFA35_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZT55883.1"
                     /translation="MRRPPQPAAAASLVPAVTRALTLLDRLAKAREPMSLAQLTHDLA
                     LPKSSVHGLCNTLMSFGYLRRQPDGAFLIGPRVMSLAEAFVAGTDVAQEFNALWADAG
                     AAPEETVVLSVLSGGDALYVAVRNSARPLGLAFNVGMRLPAYLSGSGRAMLAYREPDE
                     VRKLFAGGLNTHLTRKGPRDIEALLKELALNRKRGYSIDDEAVREGVYSFGAPVFDAS
                     GRAVAGVAVCINKVLLGADRGDRHRDAVLAVARTLSQRLGGEVKTLATVGRQRKAGGH
                     P"
     gene            complement(4696..5394)
                     /locus_tag="VFA35_06640"
     CDS             complement(4696..5394)
                     /locus_tag="VFA35_06640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZT55884.1"
                     /translation="MSEPDQQPTRILAIRHGETDWNVEHRIQGQLDVPLNEVGRWQLQ
                     RLALALADEAIDAVYSSDLLRALETAQAVARGCGLPIVTDVGLRERGFGEFEGLTYAE
                     IDAHWPALAERWRKRDPSFGTPGGETLNAFYARSVATATRLAALHPGQTIALVSHGGV
                     LDCLYRAATRVALDAPRSWQIGNAAINRLLHTPQGFTLIGWADDYHLDDGSLDESADG
                     DAPVRPAPAAKAAA"
     gene            complement(5394..>5941)
                     /locus_tag="VFA35_06645"
     CDS             complement(5394..>5941)
                     /locus_tag="VFA35_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZT55885.1"
                     /translation="TFSDNLSPHLAWSEVPAGTRSFALICHDFDVPSQGDDVNQPDRE
                     VPADLPRVDFFHWVMVDLPAACTEIAEGQFSRGFTARGKPGPAVAALPGARQGLNDYT
                     GWFAGNAEMAGDYFGYDGPFPPFNDSLIHHYVFTVYALSVERAPVEARFTGPQVREAI
                     YPYILAEATHSGTYTLNRRLI"
BASE COUNT          930 a         2002 c         2036 g          973 t
ORIGIN      
        1 cgccgaggcc gagcttggcg agcttcgagg tctggacgtc cgcggccatc acgcctcctt
       61 gccgaacagg ccagccggcc ggaccatcag cacgatcgcc atgatcacga acacgacgat
      121 gctcgaggcc tcgggataga agaccttggt caggccctcg atcaggccga gagcgaggcc
      181 ggtgaggatc gaaccgagga tcgagcccat gccgccgatc acgaccaccg cgaacacgac
      241 gatgatcagg ttgctgccca tcagcggcgt gatctggatc accggcgccg ccagcacgcc
      301 ggcgaacgcc gccagcgcgc agccgccgcc gtaggtcagc atcaccatca ccggcacgtt
      361 gatgccgaag gcctgcacca gcgtcgggtt ctcggtgccg gcgcgcaggt aggcgccgag
      421 ccgggtgcgc tcgatcaggt accaggtgcc caggcacacg gccagcgagg cgacgacaac
      481 ccacgcgcgg tagttcggca acaccatgaa gccgaggttc gtcgcgccct ggagcagatc
      541 gggcaccggg tacgactggc ccgagacgtc gaaccagtag cggaacagcc cctccgcgat
      601 cagcgccagg ccgaaggtca gcaacaggcc gtagatcggg tcgatcttgt acaggcgctt
      661 gagcagcgtg cgctcgatca cgacgccgag cgcgcccacc accagcggcg atacgatcag
      721 cgcgaaccag tagttgatgc cgaacttgtc gagcgccacg aacgcgacat acgcgccgat
      781 catgtacagc gcgccgtgcg cgaagttgac gatccccagc aacccgaaga tgaccgccag
      841 gcccaggctc agcatcgcgt agaacgagcc gttgacgagg ccgagcagca gctggcccag
      901 gaaggcctga atcgggatgc cgaagatttc catggcgtca gttcgcgtgc atcacttcca
      961 gagggcgcac ttcgattcgg ccttcgtcgt gaaggcgtcc tcgcccttgg tcgtggcgac
     1021 gacgttgtag tagtcccacg gcgtggtcga ctgcgcgggc gtcttgacct gcatcaggaa
     1081 catgtcgtgc accatgcggc cgtcggcgcg gacgtagccg cccttggcgt acatgtcgtt
     1141 gaccttgctc tcgcgcatct tcgccatcac cttctcgccg tcgtcggtcc cggccgcctt
     1201 gaccgcgttc aggtagaact gcgcgaccga gtagtcggcg gcctgcagcg acgacggcat
     1261 gcgcttgaac ttgtcgaaga agcgcttgct ccaggcgcgc gattcggccg actggttcca
     1321 gtaccagctg tcggtcaggt acatgccctg cgtcgccttc aggccgagcg agtgcacgtc
     1381 gttgatgaac atcagcaggc cggcaagctt catcgtcttc gtgacgccga actcgttcgc
     1441 cgccttgacc gcgttgatcg tgtcgccgcc ggcgttggcc agacccagga tctgcgcctt
     1501 gctcgactgc gcctgcagca ggaacgacga gaagtcgctc gccgacagcg ggtgccgcac
     1561 cgagccgacg atgctgccac cggccgcctt gacggcattc gtcgcgtcgt tctgcagcgc
     1621 ggtgccgaag gcatagtcgg cggtcaggaa gtaccagctc ttgccgccgg ccttgacgac
     1681 ggcgggcgcg gtgcccttgg cgagcgcggt cgtgtcgtag gcgtagtgga tcgtgtaggg
     1741 cgagcactgg tcgttggtca gggccgagct gcccgcgccg accgcgagga acggcttctt
     1801 cttctcggcc gcgaccttcg ccatcgccag gctggtgccg gagttggtgc ctccgatcag
     1861 cacgtccagg ccctgctggt cgaaccactc gcgagccttc gaggcggcga cgtccggctt
     1921 gttctggtgg tcggcgacca gcacttcgat cttcttgccg gcaacggtgc caccgagatc
     1981 ggcgaccgcc atgcggatcg cctcaccacc ggccgggccg tcgatgtcgg cgtacaggcc
     2041 cgacaggtcg gagatgaagc cgatcttgat cgtgtcgccc gagatctggg cctgggctga
     2101 agcgccaaag agggtggtgg cggcgagcgc gcaggcggca gagaggtggg tcaatttcat
     2161 cgaagtctcc ttgtggggga agttgcttgg ggtgatggcg ccgatcagac accgaggtat
     2221 tcgtgcaatc gttcgaggcg cgacggcagt tcggcttgcg tgaactcctg gatcacctgg
     2281 ccgtgctcca tcaccatgaa gcgatcggcc agcggcgcgg cgaaacgaaa gttctgttcg
     2341 accatcacga cggtgtagcc ctgctggcgc agcgcgatga ccatctcggc gagcttttgc
     2401 acgatcaccg gggccaggcc ctcggagatc tcgtcgagca gcagcagccg cgcgccggtg
     2461 cgcaggatgc gcgcgaccgc gagcatctgc tgctcgccgc ccgacagccg tgtgccctgg
     2521 ctgctggcac gctccttcag gttcgggaac atcgtgtaga tctgctcgac gctcatgccg
     2581 ccttcggcca gcgtcggcgg cagcatcagg ttctcctcgg ccgacaggct cgagaagatt
     2641 ccgcgctcct cggggcaata gcccacgccg agccgcgcga tgcggtgggt ggccatcgcg
     2701 atggtctcgg tgccgcgcac cttgatcgag cccttgcgcg tgccgatcag ccccatgatc
     2761 gcgcgcatcg tgctggtgcg cccggcgccg ttgcggccga gcagcgtcac gacctcgcct
     2821 tcgtcgacgc ggaagttcac gccgtgcagc acgtgcgact cgccgtacca gccttgcagg
     2881 tctttgactt cgaggaacgg agtggccatc tcagtgggcc cccttcagct cgacattcgc
     2941 gctgcccatg taggcctcga tcaccgcggg attcttcgac acttgttcat acggcccttc
     3001 ggccagcgtc gcgccgcgct gcaacacggt gatcgtgtcg gcgatgctcg agaccacgct
     3061 catgttgtgt tccaccatca gcaccgtgcg gctcgccgcg accttcttga tcagctggcg
     3121 gatgcggtcg acgtcttcga ggcccatgcc ctgcgtcggt tcgtcgagca gcatcagttt
     3181 cgggtccatc gccagcgtgg tcgcgatctc gagcgcgcgc ttgcggccgt agctgagttc
     3241 gaccgcgggg atgcgcgcat acgcctcgag atcgacctcg cgcagcagcg cgagcgcctg
     3301 cgcgtcgagc gtctcgagcg agcgctcggg cttccagaac tggtacgagg tgccgagctt
     3361 gcgctgcagc gcgacgcgca cgttctcgag caccgacagg tgcgggaaca ccgccgagat
     3421 ctggaacgag cggatcacgc cgcggcgcgc gatcgccgcc ggcttctcgg gcgtgatgtc
     3481 ggtgccgtcg aacacgatct gcccgctcgt ggggatcagg aacttcgtca gcaggttgaa
     3541 gcaggtcgtc ttgccggcgc cgttcgggcc gatcagcgca tggatgctgc cgcgcctgac
     3601 cttcaggtcg accttgctga ccgccacgaa gcccttgaac tccttcgtga gctggcgggt
     3661 ttcgagaatg aattcaccgc tcatggatgg cctccggcct tgcgttgccg accgacggtt
     3721 gccaaggttt tcacctcgcc gccgagccgc tgcgacagcg tgcgcgcgac cgcgagcacc
     3781 gcgtcgcggt gccggtcgcc gcggtcggcg ccgagcagca ccttgttgat gcagaccgcc
     3841 acgccggcga cggcccggcc actcgcgtcg aacaccggcg cgccgaacga gtacacgccc
     3901 tcgcgcaccg cttcgtcgtc gatgctgtag ccgcgtttgc ggttcagcgc gagttccttc
     3961 agcagcgcct cgatgtcgcg cgggcccttg cgcgtcaggt gcgtgttcag accgcccgcg
     4021 aacagcttgc gcacctcgtc gggctcgcgg taggcgagca tcgcgcggcc gctgcccgag
     4081 aggtacgcgg gcagccgcat gccgacgttg aacgccagcc cgagcgggcg cgcgctgttg
     4141 cgcaccgcga cgtacagcgc atcgccgccg ctgagcaccg agagcacgac ggtttcttcg
     4201 ggcgccgcgc cggcgtcggc ccacagtgcg ttgaactcct gcgccacgtc ggtgccggcg
     4261 acgaaggcct cggccaggct catcacgcgc gggccgatca ggaacgcgcc gtcgggctgg
     4321 cgccggaggt agccgaacga catcagcgtg ttgcacaggc cgtgcacgct gctcttgggc
     4381 agcgcgaggt cgtgcgtcag ttgcgccagg ctcatcggtt cgcgcgcttt cgcgaggcgg
     4441 tcgagcaagg tcagcgcgcg cgtcacggcc ggcaccagcg acgcggccgc cgcgggttgc
     4501 ggcgggcggc gcaaaggcgc gcgcggtgac aggggcgtgg cgtgcaggat cgtctccggc
     4561 ttgggtggat cgaatctcgt ggatcgaacg ttcaataggc tgaacggtgt tcaaccgatc
     4621 aggcgcgcag tctcttgcag agcggcgcgc gccgcaagcc ccgggcgcgc gtttctcggg
     4681 aaaacaccga gacggctatg cggccgcctt cgcggccggc gccggcctca ccggcgcatc
     4741 gccatcggcc gattcgtcga gcgacccgtc gtcgaggtga tagtcgtcag cccagccgat
     4801 cagcgtgaag ccctgcggtg tgtgcagcag ccggttgatc gcggcattgc cgatctgcca
     4861 cgagcgcggc gcatcgagcg caacacgcgt cgcggcgcgg tacaggcagt cgagcacgcc
     4921 gccgtgcgag accagcgcga tcgtctggcc cgggtgcaat gcggccaggc gggtcgccgt
     4981 cgcgacgctg cgcgcgtaga acgcgttcag cgtttcgccg ccgggcgtgc cgaagctcgg
     5041 gtcgcgcttg cgccagcgct cggccagcgc gggccagtgg gcatcgatct cggcgtaggt
     5101 caggccctcg aactcgccga aacctcgctc gcgcaggccg acatcggtga cgatgggcaa
     5161 gccgcagccg cgcgccaccg cctgcgcggt ctccagcgcg cgcagcaggt cgctcgaata
     5221 gaccgcgtcg atggcttcgt cggccagcgc gagcgcgagc cgctgcagct gccagcggcc
     5281 cacctcgttc aacggcacgt cgagctggcc ctggatgcgg tgctcgacgt tccagtcggt
     5341 ttcgccgtgg cggatcgcga ggatgcgcgt gggctgctgg tccggctccg acatcagatc
     5401 agccggcggt tcagcgtgta ggtgcccgag tgcgtcgcct cggcgaggat atacgggtag
     5461 atcgcctcgc gcacctgcgg cccggtgaac cgggcttcga ccggcgcgcg ctcgaccgag
     5521 agcgcgtaga cggtgaacac gtagtggtgg atcagcgaat cgttgaacgg cgggaacggg
     5581 ccgtcgtagc cgaagtagtc gcccgccatc tcggcgttgc cggcaaacca accggtgtag
     5641 tcgttcaggc cctggcgtgc gccgggcagc gccgccaccg ccgggccggg cttgccgcgc
     5701 gccgtgaagc cgcggctgaa ctggccctcg gcgatctcgg tgcaggcggc gggcaggtcg
     5761 accatcaccc aatggaagaa gtcgacccgg ggcagatcgg ccggcacttc gcggtcgggc
     5821 tggttgacgt cgtcgccctg gctcggcaca tcgaagtcgt ggcagatcag cgcgaacgat
     5881 cgcgtgccgg ccggcacctc gctccacgcc agatgcgggc tcaagttatc cgagaaggtc
     5941 a
//
LOCUS       DASRDG010000401         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_516422, whole genome shotgun sequence.
ACCESSION   DASRDG010000401 DASRDG010000000
VERSION     DASRDG010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516422"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFA35_06650"
     CDS             <1..187
                     /locus_tag="VFA35_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55886.1"
                     /translation="TTLFGFLLIAALALVMLAVTRAMQGRWTSTWVGPGGRAHGVPAR
                     GLAMEPAHAEISGMVRS"
     gene            247..2682
                     /gene="nirB"
                     /locus_tag="VFA35_06655"
     CDS             247..2682
                     /gene="nirB"
                     /locus_tag="VFA35_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HZT55887.1"
                     /translation="MRKMKLVMIGNGMAGVRTLEELLKMAPELYDITVFGAEPHPNYN
                     RILLSPVLAGEQTLDEIVLNPISWYAENGITLHLGKKVVDVDRRRRIVKAEDGTEAEY
                     DRLLICTGSNPFILPVPGKELDGVIAYRDIHDTNTMIEAATKYKHAVVIGGGLLGLEA
                     ANGLMLRGMQVTVVHVMPWLMERQLDDVSGKLLQKSLEDRGLKFLIGAQTQALIGDKD
                     GRVMAVQFKDGTEIPADLVVMAAGIRPNTTLAEKIGLHCNRGIVVDDTMQTITDPRIY
                     SVGECAAHRGIAYGLVAPLFEQGKVCATHLAHFGIGLYQGSLTSTKLKVTGIDLFSAG
                     NFMGGDGCEEIVMSDPYGGVYKKLVIKDDKLIGACMYGDTVDGSWYFKLLREGRKVAD
                     IRDKLMFGESNIGDTGHEGHSKAAAMNDADEVCGCNGVTKGAICKAIKEKGLFTLEEV
                     KKHTKASASCGSCTGLVEQLLMFTAGGDYSKAPTKKAMCGCTDASHQDVRDAIKAEHL
                     LTIDSVYKTQGWRTPNGCASCRPAVNYYLISTWPKEAKDDPQSRYINERSHANIQKDG
                     TYSVIPRMWGGETSADELRRIADAVDKYKVPTVKVTGGQRIDLLGVKKEDLVDIWKDI
                     GMPSGHAYAKALRTVKTCVGSEWCRFGTQDSTQMGKDMERAMWRMYAPHKVKFAVSGC
                     PRNCAEAGIKDVGIIGVDSGWEMYVAGNGGIKTEVAHFFTKLKTAEEVLEYTGAFCQL
                     YREEGWYLERTVHYVNRVGLDYVKKKILDDAAGRKALWERLQFALDGEPDPWFEFDKA
                     AVDTRQFTPVDVL"
     gene            2679..>3253
                     /locus_tag="VFA35_06660"
     CDS             2679..>3253
                     /locus_tag="VFA35_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HZT55888.1"
                     /translation="MKRRCFTAAATLGSAMALLPATSPAQVTDLNDAINKAGRQRMLS
                     QRMAKSYLAEGEAVVPELAQRTLSGSMALFDRQLVELKAFSPRPEIKSTYGNLEAVWS
                     DYKAALVGSKPAKSQAGAMLDLAAKVLALAHQGTVQLEKVSGRPLGHLVNVSGRQRML
                     SQRMAAFYLSISWGVQTATSTTELNKARGEFV"
BASE COUNT          636 a         1087 c         1083 g          447 t
ORIGIN      
        1 caccaccctg ttcggcttcc tcctgatcgc cgcgctcgcg ctcgtgatgc tcgccgtgac
       61 gcgtgcgatg cagggacgct ggacgagcac ctgggtcggc cccggcggcc gcgcgcacgg
      121 cgtgcccgcc cgcggcctcg cgatggaacc ggcgcacgcc gagatcagcg gcatggtgcg
      181 gtcgtgacgg cggccgtcgt cgaagcgccg cgcggcgcga atgcacaacg cgaaggagtc
      241 cccgccatga ggaagatgaa gctcgtgatg atcggcaacg gcatggccgg cgtgcgcacg
      301 ctcgaggagc tgctcaagat ggcgcccgag ctgtacgaca tcacggtgtt cggcgccgag
      361 ccgcacccga actacaaccg catcctgctc agtccggtgc tggccggcga acagacgctc
      421 gacgagatcg tgctgaaccc gatcagctgg tacgccgaga acgggatcac gctgcacctg
      481 ggcaagaagg tcgtcgacgt cgaccggcgc cgccgcatcg tgaaggccga agacggcacc
      541 gaggccgagt acgaccggct gctgatctgc accggctcga acccgttcat cctgcccgtg
      601 ccgggcaagg aactcgacgg cgtgatcgcc taccgcgaca tccacgacac caacacgatg
      661 atcgaggccg cgaccaagta caagcacgcg gtcgtgatcg gcggcggcct gctcgggctc
      721 gaagccgcca acggcctgat gctgcgcggc atgcaggtca cggtcgtcca cgtgatgccg
      781 tggctgatgg aacggcagct cgacgacgtg tcgggcaagc tgctgcagaa gtcgctcgaa
      841 gaccgcggcc tgaaattcct gatcggcgcg cagacccagg cgctgatcgg cgacaaggac
      901 ggccgcgtga tggcggtgca gttcaaggac ggcacagaga tcccggccga cctggtcgtg
      961 atggcggccg gcatccgccc gaacaccacc ctcgccgaga agatcggcct gcactgcaac
     1021 cgcggcatcg tcgtcgacga cacgatgcag accatcaccg acccgcgcat ctactcggtc
     1081 ggcgaatgcg ccgcgcaccg cggcatcgcc tacggcctgg tcgcgccgct gttcgagcag
     1141 ggcaaggtct gcgcgaccca cctcgcgcac ttcggcatcg gcctgtacca gggctcgctc
     1201 acgtcgacca agctcaaggt caccggcatc gacctgttct cggccggcaa cttcatgggt
     1261 ggcgacggct gcgaagagat cgtgatgagc gacccctacg gcggcgtcta caagaagctc
     1321 gtgatcaagg acgacaagct gatcggcgcc tgcatgtacg gcgacaccgt cgacggcagc
     1381 tggtacttca agctgctgcg cgagggccgc aaggtcgcgg acatccgcga caagctgatg
     1441 ttcggcgaat cgaacatcgg cgacaccggc cacgagggtc acagcaaggc cgcggcgatg
     1501 aacgacgccg acgaggtctg cggctgcaac ggcgtcacca agggcgcgat ctgcaaggcg
     1561 atcaaggaga aaggcctgtt cacgctcgaa gaggtgaaga agcacaccaa ggccagcgcc
     1621 tcgtgcggct cgtgtaccgg cctggtcgag cagctgctga tgttcacggc cggcggcgac
     1681 tactcgaagg caccgaccaa gaaggcgatg tgcggttgca ccgacgccag ccaccaggac
     1741 gtgcgcgacg cgatcaaggc cgagcacctg ctcaccatcg acagcgtcta caagacccag
     1801 ggctggcgca cgccgaacgg ctgcgcgagc tgccgcccgg ccgtcaacta ttacctgatc
     1861 agcacctggc cgaaggaggc gaaggacgat ccgcaatcgc gctacatcaa cgagcgcagc
     1921 cacgccaaca tccaaaagga cggcacctac tcggtgatcc cgcgcatgtg gggcggcgag
     1981 acctcggccg acgaactgcg ccgcatcgcc gacgcggtcg acaagtacaa ggtgccgacc
     2041 gtcaaggtca ccggcggcca gcgcatcgac ctgctcggcg tgaagaagga agacctggtc
     2101 gacatctgga aggacatcgg catgcccagc ggccacgcct acgcgaaggc gttgcgcacc
     2161 gtgaagacgt gcgtcggcag cgagtggtgc cgcttcggca cccaagacag cacgcagatg
     2221 ggcaaggaca tggagcgcgc gatgtggcgc atgtacgcgc cgcacaaggt caagttcgcg
     2281 gtcagcggct gcccgcgcaa ctgcgccgaa gccggcatca aggacgtcgg catcatcggc
     2341 gtcgattcgg gctgggagat gtacgtcgcc ggcaacggcg gcatcaagac cgaggtcgcg
     2401 cacttcttca ccaagctgaa gaccgccgaa gaggtgctcg aatacaccgg cgcgttctgc
     2461 cagctctacc gcgaagaagg ctggtacctg gagcgcacgg tgcactacgt gaaccgggtc
     2521 ggcctcgact acgtgaagaa gaagatcctc gacgacgcgg ccggccgcaa ggcgctgtgg
     2581 gaacggctgc agttcgcgct cgacggcgaa cccgatccgt ggttcgagtt cgacaaggcc
     2641 gcggtcgaca cgcgccagtt cacgccggtg gacgtccttt gaagcgccgt tgcttcaccg
     2701 ccgcggcaac cctgggcagc gcgatggcgc tgctgcccgc cacgtcgcct gcgcaggtca
     2761 ccgatctcaa cgacgccatc aacaaggcgg ggcgccagcg catgctcagc cagcgcatgg
     2821 ccaagagcta cctcgccgaa ggcgaggccg tggtgccgga gttggcgcag cgcacgctgt
     2881 ccggttcgat ggcgctgttc gatcgtcagc tggtcgaact gaaggcgttc tcgccgcggc
     2941 ccgaaatcaa gtcgacctac ggcaacctcg aggccgtctg gagcgactac aaggccgcgc
     3001 tggtcgggag caagccggcc aagtcgcaag ccggcgccat gctcgacctg gcggccaagg
     3061 tgctcgcgct ggcccaccaa ggcaccgtgc agctcgagaa ggtgtccggc aggccgctcg
     3121 gccatcttgt caacgtctcg ggccggcagc gcatgctgag ccagcgcatg gccgcgttct
     3181 atctcagcat cagctggggc gttcagacgg cgacatcgac caccgaactg aacaaggcac
     3241 gcggcgagtt cgt
//
LOCUS       DASRDG010000402         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1444137, whole genome shotgun sequence.
ACCESSION   DASRDG010000402 DASRDG010000000
VERSION     DASRDG010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444137"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFA35_06665"
     CDS             complement(<1..717)
                     /locus_tag="VFA35_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZT55889.1"
                     /translation="MTGAALDAAVWHALKARADAPPACPRDALWLAQAQIGSVEPALA
                     AAMVAADLPVARTGAGWRIAAEPDADAALARIAHWLHCQGLGSAWRDERLAVTDAAGT
                     PRARIERAAVRPLGIATRAVHLVGLGGEGGVWVQQRAFDKATDPGLWDTLMGGLQAAG
                     ESDAQTLERETWEEAGLRVAELIELRGAGHLIVRRPVADGYMVEHIEIVVATVPDNLV
                     PTNRDGEVARFECLARPALRQ"
     gene            complement(714..2795)
                     /gene="ligA"
                     /locus_tag="VFA35_06670"
     CDS             complement(714..2795)
                     /gene="ligA"
                     /locus_tag="VFA35_06670"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZT55890.1"
                     /translation="MPDQDPAERAAALRTELQRHAHLYYTLDDPQIPDAEYDRLFQEL
                     QAIEAVHPELLTPDSPTQRVIGAVLDGLVPVRHRVPMLSIHTETDTTSAGAEKFDARV
                     RKALALADDAPPLAYNAELKFDGLAINLRYERGELVQAATRGDGETGEDVTHTVRMIA
                     GVPKRLRGADAVQAAVLEVRGEVFMRRADFEALNARQRELIAGGAKGEKTFVNPRNAA
                     AGVVRQLDAGKAAKRPLSFFAYGLGEVLGWDEPGTQHGLGAALRKMGLPVNDDSRVVH
                     GAAGLIAYHREIAARRDALPFDIDGVVYKLDDRALQAQLGFKSREPRWAVAHKYPAQE
                     QTTVLLDIDVQVGRTGKLTPVAKLAPVFVGGTTVSNATLHNQDEIDRKDVRIGDTVIV
                     RRAGDVIPEVVGSVIEKRPVDAQAFDLVRRLAGKCPVCGSEITREEGEVDWRCSGGLF
                     CGAQRKQAILHFAGRRAMDVEGLGDKLVDQLVDAGIVRTLPELYKLGVAKLAALDRMA
                     EKSAQNLVDGLAKSKHTTLARFLYALGIRHVGETTAKDLARHFGQLDRVLDASEAQLL
                     EVADVGPIVAHSIHTFFAQPHNREVVEQLRACGISWKEDDGTADTTPKPLLGQTFVLT
                     GTLPTLTRDEAKERIEAAGGKVSGSVSKKTHFVVAGAEAGGKLEKAIELGITVLDEAA
                     LLALLPAAEAP"
     gene            complement(2826..3893)
                     /locus_tag="VFA35_06675"
     CDS             complement(2826..3893)
                     /locus_tag="VFA35_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZT55891.1"
                     /translation="MSNSLTIALASLGGVVLAGVIAHGAWAARRAGPKRADALPERTE
                     PREPVMDGVAAPAEAGDAGPTLAALHSPAPERRASKRLTPKLDALIDAIATLRLDAPI
                     GGAQAIAQLPTTRRAGGKPFIIEGLDSESGEWEAPSAGQHYGEFQAGVQLANRTGALN
                     EIEYSEFVQKIQAFADAIGAMADFPDMLEAVAGARELDHFASAHDAQLAVQLRARAAA
                     WSVGYIQQHAGRHGFVPGVVPGRLVMPAAEEGAPPVLTLTFDSQAALADDPNLAAVRE
                     VTLSFDVPQTDPSAHPFAAWQASAQALAQAMDARIVDDHGRELGTESFAAIGSELGQL
                     YAALEGRDLAAGTAAARRLFS"
BASE COUNT          493 a         1473 c         1433 g          596 t
ORIGIN      
        1 ctggcgcagc gccggccggg ccaggcactc gaagcgcgcg acctcgccgt cgcggttggt
       61 cggcacgagg ttgtcgggaa cggtcgccac gacgatctcg atgtgctcga ccatgtagcc
      121 gtcggcgacc ggccggcgca cgatcaggtg gccggcgccg cgcagctcga tcagctcggc
      181 gacgcgcagc ccggcctcct cccaggtctc gcgttcgagc gtctgcgcgt cggactcgcc
      241 ggccgcttgc agcccaccca tcagcgtgtc ccacaggccc gggtcggtgg ccttgtcgaa
      301 cgcgcgctgc tggacccaga cgccgccctc gcctccgagg ccgaccaggt gcaccgcgcg
      361 cgtcgcgatg cccagcggcc gcaccgcggc acgctcgatg cgcgcgcgcg gcgtgccggc
      421 ggcatcggtc acggcgagcc gttcgtcgcg ccacgccgag cccaggccct ggcaatgcag
      481 ccagtgcgcg atgcgcgcga gcgcggcgtc ggcgtccggc tcggcagcga tgcgccagcc
      541 cgcgccggtg cgtgccaccg gcaagtccgc ggcgaccatc gccgcggcca gcgcgggttc
      601 caccgagccg atctgcgcct gcgcgagcca cagcgcatcg cgcgggcacg ccggcggcgc
      661 atcggcgcgt gccttcagcg cgtgccacac ggccgcgtcg agcgcggcgc cggtcatggt
      721 gcctcggccg ccggcagcag cgccagcagc gccgcctcgt cgagcaccgt gatgccgagc
      781 tcgatcgcct tctccagctt gccgcccgcc tcggcgccgg cgacgacgaa gtgggtcttc
      841 ttcgagaccg agccgctgac cttgccgccg gcggcttcga tgcgctcctt ggcctcgtcg
      901 cgcgtcaggg tcggcaaggt gccggtcagc acgaaagtct ggcccagcag cggcttcggc
      961 gtggtgtcgg cggtgccgtc gtcttccttc cagctgatgc cgcaggcgcg caattgctcg
     1021 acgacctcgc ggttgtgcgg ctgcgcgaag aaggtgtgga tgctgtgcgc gacgatcggc
     1081 ccgacatcgg cgacctcgag cagctgcgcc tcgctcgcat cgagcacgcg gtcgagctgg
     1141 ccgaagtgcc gggcgagatc cttcgcggtc gtctcgccga cgtggcggat gccgagcgca
     1201 tacagaaagc gcgccagcgt cgtgtgcttg ctcttcgcca gcccgtcgac caggttctgc
     1261 gcgctcttct cggccatgcg gtcgagcgcc gcaagcttgg cgacgccgag cttgtagagc
     1321 tcgggcagcg tgcgcacgat gccggcatcg acgagctggt cgaccagctt gtcgcccagc
     1381 ccttcgacat ccatcgcgcg ccgccccgcg aagtgcagga tcgcctgctt gcgctgcgcg
     1441 ccgcagaaca ggccgcccga gcagcgccag tcgacctcgc cctcctcgcg cgtgatctcg
     1501 ctgccgcaca ccgggcattt gccggctagc cgccggacca gatcgaacgc ttgcgcatcc
     1561 accgggcgct tctcgatgac cgagccgacg acctcgggga tcacgtcgcc ggcgcgccgc
     1621 acgatcacgg tgtcgccgat gcgcacgtcc ttgcggtcga tctcgtcctg gttgtgcagc
     1681 gtcgcgttgc tcaccgtcgt gccaccgacg aacaccggcg ccagcttcgc gaccggcgtg
     1741 agcttgccgg tgcggccgac ctgcacgtcg atgtccagca gcacggtggt ctgctcctgc
     1801 gccgggtact tgtgcgcgac cgcccagcgc ggctcgcgcg acttgaagcc gagctgcgcc
     1861 tgcagcgcgc ggtcgtcgag cttgtagacg acgccgtcga tgtcgaacgg cagcgcgtcg
     1921 cggcgcgccg cgatttcgcg gtgataggcg atcaggccgg ccgcgccgtg cacgacgcgg
     1981 ctgtcgtcgt tgaccggcag gcccatcttg cgcagcgccg cgccgaggcc gtgctgcgtg
     2041 cccggctcgt cccagccgag gacctcgccc agcccgtagg cgaagaagct cagcgggcgc
     2101 ttcgcggcct tgccggcatc gagctggcgc accacgccgg ccgcggcgtt gcgcgggttc
     2161 acgaaggtct tctcgccctt cgcaccgccc gcgatcaact cgcgctggcg cgcgttcagc
     2221 gcctcgaagt cggcgcgccg catgaagacc tcgccacgca cttcgagcac cgcggcctga
     2281 actgcatccg ccccgcgcag gcgcttcggc acgccggcga tcatgcgcac ggtatgggtc
     2341 acgtcctcgc cggtttcgcc gtcaccgcgc gtggccgcct gcaccagttc gccgcgctcg
     2401 tagcgcaggt tgatcgcgag gccgtcgaac ttcagctcgg cgttgtaggc cagcggcggc
     2461 gcgtcgtcgg ccagcgcgag cgccttgcgc acgcgcgcgt cgaacttctc ggcgccggcc
     2521 gacgtggtgt cggtctcggt gtggatcgac agcatcggca cgcggtgccg caccggcacc
     2581 aggccgtcga gcaccgcgcc gatcacgcgc tgggtcggcg aatcgggcgt cagcagctcg
     2641 gggtgcacgg cctcgatcgc ctgcagctcc tggaacaggc ggtcgtactc ggcgtcgggg
     2701 atctgcgggt cgtcgagcgt gtagtagagg tgggcgtgac gctgcaactc cgtgcgcagc
     2761 gccgccgcgc gttcggcggg atcttggtcg ggcatggccc gaagtttaga caggccggcc
     2821 ggggttcaac tgaacagccg ccgcgccgcc gccgtgccgg cggcgaggtc gcggccttcg
     2881 agcgcggcat agagctggcc gagctcgctg ccgatcgccg cgaagctttc ggtgccgagc
     2941 tcgcggccgt ggtcgtcgac gatgcgcgcg tccatggctt gcgccagcgc ctgcgcgctc
     3001 gcctgccagg ccgcgaacgg gtgcgcggag gggtcggtct gcggcacgtc gaagctcagc
     3061 gtcacctcgc gcaccgcggc gaggttgggg tcgtcggcga gcgcggcctg cgagtcgaag
     3121 gtgagcgtga gcacgggcgg tgcgccctct tcggccgccg gcatcacgag ccggccgggc
     3181 acgacgccgg ggacgaagcc gtggcgcccg gcatgctgct ggatgtagcc gacactccac
     3241 gccgccgcgc gcgcgcgcag ctgcaccgcg agctgcgcgt cgtgggcgct cgcgaagtgg
     3301 tcgagctcgc gcgcgccggc gacggcttcg agcatgtcgg gaaagtcggc catcgcgccg
     3361 atcgcatcgg cgaaggcctg gatcttctgc acgaactcgg agtactcgat ctcgttcagc
     3421 gcgccggtgc ggttggcaag ctgcacgccg gcctggaact cgccgtagtg ctggccggcg
     3481 gacggcgcct cccattcgcc cgattcgctg tcgagccctt cgatgatgaa gggcttgccg
     3541 ccggcgcggc gcgtggtcgg cagctgcgcg atcgcctgcg cgccgccgat cggcgcatcc
     3601 aggcgcagcg tggcgatcgc gtcgatcagc gcgtcgagct tcggcgtcag gcgcttgctg
     3661 gcgcggcgct ccggcgcagg cgaatgaagt gcggcgagcg tcggcccggc atcacccgcc
     3721 tcggccggtg ccgcaacgcc gtccatcacc ggctcgcgcg gctcggtgcg ctcgggcagc
     3781 gcgtcggcgc gcttcggccc ggccctgcgc gcggcccacg cgccgtgcgc gatcacgccc
     3841 gcgagcacga cgccgcccag ggatgccagc gcgatcgtca agctgttgct catggccgga
     3901 ttttggttca ggccgcctcg gcgaggctca cggccgcgtc catgtcgacc gtcacgatgc
     3961 gcgagacgcc ctgctcctgc atcgtcacgc cgatc
//
LOCUS       DASRDG010000403         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_174919, whole genome shotgun sequence.
ACCESSION   DASRDG010000403 DASRDG010000000
VERSION     DASRDG010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174919"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1802
                     /locus_tag="VFA35_06680"
     CDS             186..1802
                     /locus_tag="VFA35_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZT55892.1"
                     /translation="METAAPSRAEIPLHELLRAHARQTPDKPACLWYGQAVSFAELDR
                     ASDAFAARLQALGVNKGDTVALFLGNCPQYLMAQMGIQKIGAIASPVSPLNKAHELSY
                     QLEDGGARVIVASDALLPIVREVRAATALEHVFTTGYAELLPAEPTLTLPDELRALQA
                     ETRTVPADALDFLEATRSGARPQPVAIDMDDVSLMVYTSGSTGRPKGAMLSYRNASYK
                     TAAVADCNGLTSDDVLLSIAPLYHIAGMVMGVDTPILAGTTAVLLYRFDPLTVLQAIA
                     RHRVSWWYSIAPMNVAMMALPEARTHDLSSLRINPVTSFGIDWTEKLANEWRGVAKNC
                     ASMEASYGLSETHTVDTYMPRDAVRWGTHGKPVPGNEIRILDPDTGHERPRGETGEIV
                     LRSPGVFKGYWKRPDATEQTLRDGWVHTGDMGRIDAEGYLTFMGRFKEMIKVSGYSVF
                     PEEVEAILNKHPAVRQSAVIGIPDPTKGEVVKAFIVLHEGQVLDAAALVAWSRENMAS
                     YKAPRELRFLDALPATGAGKVLRRLLKDLP"
     gene            1805..>2704
                     /locus_tag="VFA35_06685"
     CDS             1805..>2704
                     /locus_tag="VFA35_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZT55893.1"
                     /translation="MFEKILVANRGDVALRVERAARDLGLRSVAVYARDDANGLHVRS
                     ADTAVALAATGPAAYLDIAALIEIARTQGCDAVHPGWGFLSERADFARACAAAGLTFI
                     GPDPEHLALFGDKARARELAQRCEVPLLPGSTGAVSLAEAQAFFAAQQARSGGAAVGV
                     MIKALGGGGGRGMRAVLDARDLPEAYARCTSEARAAFGVEGVYVERLMTRARHVEVQV
                     LGDGQAVIALGERECSLQRRFQKLVEIAPSPTLPEPLRRDIVEAALRLAQAVGYKSLG
                     TFEFLVDEQASDLPFVFMETNPRL"
BASE COUNT          406 a          938 c          972 g          388 t
ORIGIN      
        1 acgacgcgtc gacggtgctc agatgatcca tgccgttgtc tccgtggggt gcgctgatgc
       61 ggtcgcggtg cgatcagggt tgttcgcgat gacgccgctg tgtaccatgc agtacaaatc
      121 ttctaccgaa cggtataggg atgcaagccc cgccgcccac gcgtttaccc tgaaggagag
      181 tcgccatgga gacagccgcg ccgagccgcg ccgaaattcc gctgcacgag ctgctgcgcg
      241 cgcatgcgcg ccagaccccc gacaagcccg cctgcctgtg gtacgggcag gccgtgtcct
      301 tcgccgagct cgaccgcgcc agcgacgcct tcgcggcccg gctgcaggcg ctcggtgtga
      361 acaagggcga cacggtcgcg ctgttcctcg gcaactgccc gcagtacctg atggcgcaga
      421 tgggcatcca gaagatcggc gcgatcgcca gcccggtgag cccgctgaac aaggcgcacg
      481 agctgtcgta ccagctcgag gacggcggcg cgcgcgtcat cgtcgccagc gacgcgctgt
      541 tgccgatcgt gcgcgaagtg cgcgccgcga ccgcgctcga gcatgtcttc acgaccggct
      601 acgccgagct gttgccggcc gagccgacgc tcacgctgcc cgacgaactg cgcgcgctgc
      661 aggccgagac gcgcacggtg ccggccgatg cgctcgactt cctcgaggcg acgcgcagcg
      721 gtgcgcgccc gcagccggtg gcgatcgaca tggacgacgt ctcgctgatg gtctacacct
      781 cgggcagcac cggccggccg aagggcgcga tgctgagcta ccgcaacgcc tcgtacaaga
      841 ccgcggcggt cgccgactgc aacggcctga cctccgacga tgtgctgctg tcgattgcgc
      901 cgctgtatca catcgccggc atggtgatgg gcgtggacac gccgatcctc gccggcacga
      961 ccgcggtgct gctctatcgc ttcgacccgc tgacggtgct gcaggcgatc gcgcggcatc
     1021 gcgtcagctg gtggtacagc atcgcgccga tgaacgtcgc gatgatggcg ctgcccgagg
     1081 cgcggacgca cgacctgtcg tcgctgcgca tcaacccggt gacgagcttc ggcatcgact
     1141 ggaccgagaa gctcgcgaac gagtggcgcg gcgtcgcgaa gaactgcgcc tcgatggagg
     1201 cgtcgtacgg cctgtcggag acgcacaccg tcgacaccta catgccgcgc gacgcggtgc
     1261 gttggggcac gcacggcaag cccgtgcccg gcaacgagat ccgcatcctc gacccggaca
     1321 ccgggcacga gcggccgcgc ggcgaaaccg gcgagatcgt gctgcgcagc cccggcgtgt
     1381 tcaagggcta ctggaagcgg cccgacgcga ccgagcagac cctgcgcgac ggctgggtgc
     1441 acaccggcga catgggccgc atcgacgccg agggctacct gaccttcatg ggccgcttca
     1501 aggagatgat caaggtgtcg ggctacagcg tgttccccga ggaggtcgag gcgatcctca
     1561 acaagcatcc ggcggtgcgc caatcggcgg tgatcggcat cccggacccg accaagggcg
     1621 aggtcgtcaa ggctttcatc gtgctgcacg aagggcaggt gctcgatgcc gcggcgctgg
     1681 tcgcgtggtc gcgcgagaac atggcgagct acaaggcgcc gcgcgagctg cgctttctgg
     1741 acgcgctgcc cgccaccggt gccggcaagg tgctgcgccg gctgctgaag gatctgccgt
     1801 gagaatgttc gagaagatcc tggtcgccaa ccgcggcgat gtcgcgctgc gcgtcgagcg
     1861 cgccgcgcgc gacctggggc tccgctcggt cgccgtctac gccagggacg acgcgaacgg
     1921 gctgcacgtg cgcagcgccg acacggccgt ggcgctggcc gccaccggcc cggcggccta
     1981 tctcgacatc gccgcgctga tcgagatcgc acgcacgcag ggctgcgacg cggtccaccc
     2041 cgggtggggg tttctgtcgg agcgcgccga cttcgcgcgc gcctgcgccg cggccggctt
     2101 gaccttcatc ggccccgatc ccgagcacct ggcgctgttc ggcgacaagg cgcgcgcgcg
     2161 cgagctggcg cagcgctgcg aggtgccgct gctgccgggc agcaccggcg cggtgagcct
     2221 ggccgaagcg caggccttct tcgccgcgca gcaggcgcgc tccggcggcg cggcggtcgg
     2281 cgtgatgatc aaggcgctcg gcggcggcgg cgggcgcggc atgcgcgccg tgctcgacgc
     2341 gcgcgacctg cccgaggcgt atgcgcgctg cacgtccgag gcgcgcgcgg ccttcggcgt
     2401 cgagggcgtc tacgtcgagc gcctgatgac gcgcgcgcgc catgtcgagg tgcaggtgct
     2461 cggcgacggc caggccgtca tcgcgctggg cgagcgcgaa tgctcgctgc agcggcgctt
     2521 ccagaagctg gtcgagatcg cgcccagccc gacgctgccc gagccgctgc gccgcgacat
     2581 cgtcgaggcc gcgctgcggc tggcgcaagc ggtcggctac aagagcctgg gcaccttcga
     2641 gttcctggtc gacgaacagg cgagtgatct gccgttcgtc ttcatggaga cgaatccgcg
     2701 gctg
//
LOCUS       DASRDG010000404         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_174935, whole genome shotgun sequence.
ACCESSION   DASRDG010000404 DASRDG010000000
VERSION     DASRDG010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174935"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="VFA35_06690"
     CDS             complement(<1..2022)
                     /locus_tag="VFA35_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HZT55894.1"
                     /translation="MPTPANPTALAQQARRSYVEGLLSGLPTVVQAVDKGARILISQV
                     AEPSVAVKRRELVPDLQKAAPYWLQGMTSMLRGARHTGLVSATRPGDLPVSPTRSAKL
                     SLVDDDTIEHEIVSSKLALAMMDRSSWEFTDLRSRMNSLEGRDELDTNDVLRPHVFSR
                     IVTASWRAAGLNLDTWRQLQPVLHEEFAHFAEEGYHETNRFLLERHVLPEIDLRPFIR
                     RSHGGVYTGGFTHSGQGGSGGFVASSGAESRRGDGVSDETRMMTRAASLARGADHAEA
                     VLGRLNRLVGRQLPGFGVTQHDQPVMTTPRLKTAISDAQQSIARRLASAEHARTVAGG
                     HVSTPALLEELNQRKQALKQAASTPVERATIEIVALLFQSILTEERIPSAVRVWFARL
                     QMPVLRVAVSEPDFFATIDHPARRLIDRMGSCVMGFDATSRAVGDILEKEIKRVVQVV
                     EAYPDTGRRVFQTVLVEFEKFLEHYFKNENEASRKGVSLAQQVEQRETLAIQYTIELR
                     KMLNEVPVQEGVREFLFHVWADVLAMTALKHGNQSDATKDMKRVAADLIWSAGAKVSR
                     EERAEVIRRLPPLLKTLRDGMEHAGVPADKQDEHIQRLNNSLAAAFTAKTAAIPHERL
                     EELMARLETLEELLPEAEDLEIDESMVHDLSGHESSELEVVAEGGSMPTP"
     gene            complement(2061..>2280)
                     /locus_tag="VFA35_06695"
     CDS             complement(2061..>2280)
                     /locus_tag="VFA35_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZT55895.1"
                     /translation="TQMMVLSMLIFGGPTLHGFAVALTIGILFGIYSSVFVAAAIAMW
                     LGVKREDLIKSGPAKETDPDDPNAGAVV"
BASE COUNT          329 a          784 c          775 g          392 t
ORIGIN      
        1 cggcgtgggc atcgagccgc cctccgcgac aacctcgagc tccgagcttt cgtggcccga
       61 caggtcatgc accatcgact cgtcgatctc gaggtcttcg gcctcgggca gcagttcttc
      121 gagcgtctcg agccgcgcca tcagctcttc gaggcgctcg tgcgggatcg ccgcggtctt
      181 cgcggtgaac gccgcggcga gcgagttgtt caggcgctgg atatgctcgt cttgcttgtc
      241 ggccggcacg cccgcgtgct ccatgccgtc acgcaaggtt ttcagcagcg gcggcaggcg
      301 gcggatcacc tcggcgcgct cctcgcgcga cacctttgca ccggccgacc agatcaggtc
      361 ggcggcgacg cgtttcatgt ccttggtcgc gtcgctctgg ttgccgtgct tgagcgcggt
      421 catcgccagc acatcggccc agacgtggaa caggaactcg cgcacgccct cttgcaccgg
      481 cacctcgttg agcatcttgc gcagctcgat cgtgtactgg atcgccagcg tctcgcgctg
      541 ctcgacctgc tgcgccagcg acacgccctt gcgtgaggcc tcgttctcgt tcttgaagta
      601 gtgctcgagg aatttctcga actcgaccag cacggtctgg aacacgcgcc ggccggtgtc
      661 gggatacgcc tcgacgacct gcaccacgcg cttgatctcc ttctcgagga tgtcgccgac
      721 cgcgcggctg gtcgcgtcga aacccatcac gcacgagccc atgcggtcga tcaggcggcg
      781 cgccggatgg tcgatggtcg caaagaaatc gggctcgctg acggccaccc gcagcaccgg
      841 catctgcagc cgcgcgaacc agacgcgcac cgcgctcggg atgcgctctt cggtgaggat
      901 gctctggaac agcagcgcga cgatctcgat cgtcgcacgc tcgaccggcg tgctcgcggc
      961 ctgcttcagg gcctgcttgc gctggttgag ttcttcgagc agcgccggcg tgctgacgtg
     1021 gccgcccgcg accgtgcggg cgtgctcggc cgacgcgagg cgccgggcga tgctctgctg
     1081 cgcatcgctg atcgcggtct tcaggcgcgg ggtggtcatc accggttggt cgtgctgggt
     1141 cacgccgaaa cccggcagct ggcgtccgac caggcggttc aggcggccga gcacggcttc
     1201 ggcgtgatcg gcgccgcggg cgaggctggc ggcgcgcgtc atcatgcggg tctcgtcgct
     1261 gacaccgtcg ccgcgacggc tctccgcgcc gctcgatgcg acgaagccgc cgctgccgcc
     1321 ctgacccgag tgggtgaagc cgccggtgta gacgccgccg tgcgaacgcc ggatgaacgg
     1381 ccgcagatcg atctcgggca agacgtggcg ctcgagcagg aagcggttcg tctcgtggta
     1441 gccctcttcg gcgaagtgcg cgaactcttc gtgcagcacc ggctgcaact ggcgccaggt
     1501 gtccaggttc agccccgcgg cgcgccacga cgccgtgacg atgcgggaaa acacgtgtgg
     1561 ccgcagcacg tcgttggtgt cgagttcgtc acggccctcg agcgagttca tgcgcgagcg
     1621 caggtcggtg aactcccacg acgagcggtc catcatcgcc agcgccagct tcgagctgac
     1681 gatctcgtgc tcgatcgtgt cgtcgtcgac gaggctcagc ttggcactgc gtgtcggcga
     1741 caccggcagg tcgccggggc gggtggccga aaccagcccg gtgtggcgcg cgccgcgcag
     1801 catcgaggtc atgccctgca gccagtacgg cgcggccttc tgcagatcgg gcaccagctc
     1861 gcggcgcttg accgcgacgc tcggctcggc cacctggctg atcagaatcc gcgcgccctt
     1921 gtcgaccgcc tgcaccacgg tcggcaggcc ggatagcaag ccctccacgt aggagcgcct
     1981 tgcctgctgg gccaatgccg tcgggttggc gggggtcggc atgtgggagg gtcgcgtcct
     2041 gtgggatgtt gcgggtgact ctacaccacc gccccggcgt tcgggtcgtc gggatcggtc
     2101 tccttggccg gcccggactt gatcagatcc tcgcgtttga cgcccagcca catcgcgatc
     2161 gccgcggcga cgaacaccga cgagtagatg ccgaacaaga tgccgatcgt cagcgccacc
     2221 gcgaagccat gcagcgtcgg cccgccgaag atcagcatcg acagcaccat catctgcgtg
//
LOCUS       DASRDG010000405         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_633257, whole genome shotgun sequence.
ACCESSION   DASRDG010000405 DASRDG010000000
VERSION     DASRDG010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633257"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFA35_06700"
     CDS             <1..272
                     /locus_tag="VFA35_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55896.1"
                     /translation="NVDFPAVAKTMKPGTAVSATIDGLQFAFSLPRRAPAIAAQIDGH
                     RDLRAIHEGLAATNRELTWDAFLAEFVAFYAAFHALGKLVLRVPA"
     gene            complement(595..1755)
                     /locus_tag="VFA35_06705"
     CDS             complement(595..1755)
                     /locus_tag="VFA35_06705"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HZT55897.1"
                     /translation="MSEPPPLDAKWLKLVTGLPATVDLEASASQCGALKRRRKIKDAD
                     SLLRVVMINAVGHLSLRGTAAWATMQGLKLSNPAVLKRLKGSANWLGYLVAAALADRM
                     PVERMPAGVQASAGYRLRLIDATTLSAPGSHGTDWRLHVAYDLEQMRISEVELTDGHG
                     SESLTRFNLQPGDLAIADRGYAKPLDLAEVEAKGADYIVRTGWNATRLRAESGGDFDL
                     LAAVGDVTEHAVREFQLTIALDRSEKQLVPVRLVALRKNDTAAEASIRRARKESRRHG
                     KTPQRETLEAAKYVFLLTSLTPDDAPAEEILALYRLRWQIELLFKRLKSLLRIDELPN
                     KHPEAARCWILANLLAALLVEDQARLLLDAPPSAPRCPRRRTLAVARPAAVA"
BASE COUNT          357 a          790 c          767 g          395 t
ORIGIN      
        1 tcaacgtcga tttcccggca gtggcgaaga ccatgaagcc cggcaccgcg gtcagcgcca
       61 cgatcgacgg cctgcagttc gccttctcgc tgccgcgacg cgcaccggcg atcgcggcgc
      121 agatcgacgg ccaccgtgac ctgcgcgcga tccacgaggg cttggccgcg acgaatcgcg
      181 agctcacctg ggacgcattc ctcgcggagt tcgtcgcgtt ctacgccgcg ttccacgcgc
      241 tcgggaagct ggtgctgcgg gtgccggcgt agcgcttcca ttgcacggga atgctggtcc
      301 aggggagcgc gatgtcgccg cattcgcggg gcgggtcctc ccctgagcgt cagtcgaagg
      361 gccgcccgcg cgggcgaagc ccgctatggt gtgtcggttg agctaccgcc ggccttcggc
      421 cgggcgggcg ggccgagggc ccatatgcgc taaccttggt agcgtggcgg aaccaggccc
      481 tcaagctgtc gcttgcgttg tcgtcgagga tcgacgaggt ggcgacgaag atctggcgca
      541 gtgtcggcaa tgcgcgcgag gtcgagagga ccgatgacgg tggcgacgat cgcctcaagc
      601 aacagccgct ggacgcgcca cagcgagtgt acgccggcgc gggcagcgag gcgcagaggg
      661 gggagcgtcc aggagaagcc tggcctgatc ttcgacgagc agcgcggcaa ggaggttagc
      721 gaggatccaa catcgtgcgg cctcaggatg cttgtttggc agctcatcga tacgcagcag
      781 gctcttgagg cgcttgaaga ggagctcgat ctgccagcgc agtcgataga gcgcgagaat
      841 ctcttcggcc ggggcgtcgt cgggcgtgag cgaggtgaga aggaagacat acttggccgc
      901 ctcgagggtt tcgcgctggg gcgtcttgcc atgacgccgg ctttccttgc gcgcccgccg
      961 gatgctggcc tccgccgcag tgtcgttctt gcgcaacgcg accaggcgca ccggcacgag
     1021 ctgcttctcg ctgcggtcga gcgcgatggt cagctggaac tcgcgcaccg cgtgctcggt
     1081 cacatcgccc accgcggcca gcaaatcgaa gtcgccgccg ctctctgcgc gcaggcgcgt
     1141 cgcgttccat cccgtgcgca cgatgtaatc ggcgcctttt gcttctacct ccgccaagtc
     1201 gagcggcttg gcatagccac ggtcggcgat ggccagatcg ccgggctgga gattgaaccg
     1261 cgtcaggctt tcgctgccgt ggccgtccgt cagctcgacc tcgctaatcc gcatctgttc
     1321 caggtcgtag gccacatgca gccgccagtc cgtaccatgg ctgcccggcg ccgagagggt
     1381 cgtggcatcg atcaggcgca gacgatagcc cgcgctggcc tgcacgcccg ccggcatccg
     1441 ctccacaggc atccggtccg ccagcgccgc ggccacgaga tagcccaacc agtttgccga
     1501 gcccttgagg cgcttcagca ccgccgggtt cgacagcttc agcccctgca tcgttgccca
     1561 agccgccgtg ccacgcagcg acagatgccc cactgcgttg atcatcacca cgcgcagcag
     1621 actatccgcg tctttgatct ttcgtcgccg tttcagcgcc ccgcattggc tggcgcttgc
     1681 ttcgagatcg accgtcgctg gcaaccccgt taccagcttc agccacttgg catccagggg
     1741 aggcggttcg ctcatccctc tctacgaatc atcccgcccg tggtcgccgc aagcacaatc
     1801 cgccgatgcc atacctctcg acggcggatc gattctgttc tgtcgtctgt ccgctctgct
     1861 cgattccaat attgccgatc gcacccaaag tttgcgcaca ttggccgagg gcccgcccct
     1921 acgacagcgg cgcggtccgc tcgcgatttt cagccatgtg ccgggcggcc cggtgcgttg
     1981 gccgcgtacc atgaaaatgc ggtgcgatgt ccccgcccat gtaggggtcg agcttgctcg
     2041 accccggcgg ccgaaggccg ccgatcgcgc gccgttgccg gcctttggcc ggcggggccg
     2101 agcaagctct gagcaagctc ggcccccaca tcagcggagc ggtccgctcg cgatcgcgaa
     2161 gccccgcccc ctactggaac aggcgcccga tgaagcccgg gcgatcggag ctgggcaggc
     2221 gacccgtctc gatcagcgcg taggagtcgg cgtaccattc gctgcccggg tagttgaagc
     2281 cgagcaccga ggcggtgcgg cgcgcctcg
//
LOCUS       DASRDG010000406         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_769824, whole genome shotgun sequence.
ACCESSION   DASRDG010000406 DASRDG010000000
VERSION     DASRDG010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769824"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..494
                     /locus_tag="VFA35_06710"
     CDS             45..494
                     /locus_tag="VFA35_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55898.1"
                     /translation="MENIAVFVNDVAAARHLLQPMLNGAGPTRWVLVACPPTLTRHIG
                     RWVSHSARRQWRERWAAELFDALQPELKAHSGSEVETLVAKRPLVDVSARLLARMPQL
                     RLLDARQSRVGRVDELISATQPAAAASGWVAPAAATAGLSLMLTLAD"
     gene            576..1652
                     /locus_tag="VFA35_06715"
     CDS             576..1652
                     /locus_tag="VFA35_06715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZT55899.1"
                     /translation="MPLQFGAEGWLAVALVVMVVVGAVFGIGWWVGTRRPAPAAEPAP
                     TLPLDPAPALRPDRSTLGRYRIERELGRGSMGTVYLGRDPQTGRAVAIKTMALGREFE
                     GAELAEARARFFREAEMAGRLRHPDIVTIFDASEDQGLAFIAMEFVGGHDLLRHTLPG
                     RLLPVPLVLRTLARVAQALAYAHREGVVHRDVKPANVMIDPATGAVKVTDFGIARIAD
                     ACRTRTGLVLGTPAFMSPEQMAGRRVDGRTDLYSLGVMLFQMLTGALPHASESMAGLM
                     HSIVNDTAPDVRLLRPELPEALANVVALALEKRAALRYADGREMADDLLAVAQRLPPG
                     DPDSGGFGAAPESGRGEPRHNADH"
     gene            1685..>2102
                     /locus_tag="VFA35_06720"
     CDS             1685..>2102
                     /locus_tag="VFA35_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZT55900.1"
                     /translation="MTLEFFSATDTGRARNNNEDSVAVDDATALVVLADGMGGYNAGE
                     VASGMVTAFIKTELGRWLAEASETATDADVRRAMDICVDNANRAIFNAANSNPQYAGM
                     GTTLVVGVFRDARLLFGHVGDSRGYRLRAARLTQLTH"
BASE COUNT          290 a          800 c          731 g          281 t
ORIGIN      
        1 caccgccacc tgaaacgccg cccagtcaac caggagaact tcaaatggaa aacatcgccg
       61 tgttcgtcaa cgacgtggcc gccgcgcgcc acctcctgca accgatgctg aacggcgcag
      121 ggccgacgcg ctgggtgctg gtcgcctgcc cgccgacgct gacccgccac atcggccgct
      181 gggtgtcgca cagcgcgcgc cggcaatggc gcgagcgctg ggccgcggag ctgttcgacg
      241 cgctgcagcc cgagctgaag gcacattccg gcagcgaggt cgagaccctg gtcgcgaagc
      301 ggccgctggt cgacgtgtcg gcgcgcctgc tcgcgcgcat gccgcagctg cgcctgctcg
      361 acgcgcgcca gtcgcgcgtc ggccgtgtcg acgagctgat cagcgccacg cagccggccg
      421 ccgccgcgag cggctgggtc gcgccggcgg cggccacggc gggcttgtcg ctgatgctga
      481 cgctggccga ctgaccgggg cgcgtgctaa cgtgcctgcg cgatgagcag gcacgttctc
      541 caccccctcc gcgccgccgc gcgcacgccg cgctgatgcc gctgcaattc ggcgccgagg
      601 gctggctcgc cgtcgcgttg gtggtgatgg tcgtggtcgg cgcggtcttc ggcatcggct
      661 ggtgggtcgg cacacgccgg ccggcgccgg ctgcggagcc cgcaccgacg ctgccgctcg
      721 accccgcccc ggccctgcgc cccgaccgca gcacgctcgg ccgctaccgc atcgagcgcg
      781 aactcggccg cggctcgatg ggcacggttt acctcggccg cgacccgcag accggccgcg
      841 ccgtcgcgat caagacgatg gcgctcggcc gcgagttcga gggcgccgag ctggccgaag
      901 cgcgcgcgcg cttcttccgc gaggccgaga tggccgggcg cttgcgccac cccgacatcg
      961 tgacgatctt cgacgccagc gaagaccagg gcctggcctt catcgcgatg gaattcgtcg
     1021 gcggccacga cctgctgcgc catacgctgc ctggccggtt gctgccggtg ccgctcgtgc
     1081 tgcgcacgct cgcgcgcgtc gcgcaggcgc tcgcgtatgc gcaccgcgag ggcgtggtgc
     1141 accgcgatgt caagccggcc aacgtgatga tcgacccggc caccggcgcc gtgaaggtca
     1201 ccgacttcgg catcgcgcgc atcgccgacg cctgccgcac ccgcaccggg ctcgtgctgg
     1261 gcacgcccgc gttcatgtcg cccgagcaga tggccgggcg ccgcgtcgac ggccgcaccg
     1321 acctctattc gctcggcgtg atgctgttcc agatgctcac cggcgcgctg ccgcacgcgt
     1381 cggagtcgat ggccgggctg atgcacagca tcgtcaacga caccgcgccc gacgtgcgct
     1441 tgctccgccc cgagctgccc gaggcgctcg ccaacgtggt cgcgctggcg ctcgaaaagc
     1501 gcgccgcgct gcgctacgcc gacggccgcg agatggccga cgacctgctc gcggtggcgc
     1561 agcggctgcc gccgggcgac cccgattcgg gcggattcgg ggccgcgccg gagtccggcc
     1621 ggggcgaacc gaggcacaat gccgaccact gatcagagct ctcatgcccg cacacgccac
     1681 gtccatgaca ctcgagttct tcagcgccac cgacaccggc cgcgcgcgca acaacaatga
     1741 ggactcggtc gcggtcgacg acgccaccgc gctggtcgtg ctcgccgacg gcatgggcgg
     1801 ctacaacgcg ggcgaagtcg ccagcggcat ggtgaccgcc ttcatcaaga ccgaactcgg
     1861 ccgctggctc gccgaagcct ccgagaccgc gaccgacgcc gacgtgcgcc gcgcgatgga
     1921 catctgcgtc gacaacgcga accgcgcgat cttcaacgcc gccaattcca acccgcagta
     1981 cgccggcatg ggcacgacgc tggtggtcgg cgtgttccgc gatgcgcggc tcctgttcgg
     2041 gcacgtgggc gactcgcgcg gctaccgctt gcgcgcggcg cggctgacgc agctcaccca
     2101 cg
//
LOCUS       DASRDG010000407         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_422504, whole genome shotgun sequence.
ACCESSION   DASRDG010000407 DASRDG010000000
VERSION     DASRDG010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422504"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /gene="guaB"
                     /locus_tag="VFA35_06725"
     CDS             complement(<1..1387)
                     /gene="guaB"
                     /locus_tag="VFA35_06725"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZT55901.1"
                     /translation="MRLLGKALTFDDVLLVPAFSQVLPRDTDLSTPLSRNIRLNLPLV
                     SAAMDTVTEARLAIALAQEGGIGIVHKNLTPQQQAAEVARVKRYESGVLRDPITVTPD
                     TPVRAVMELSRQRGVSGFPVLQGTTVVGIVTNRDLRFESRLDAPVSEIMTPRERLVTV
                     PESATIEDGKALMHKHKLERVLVVNRDFELRGLMTVKDITKQTSFPNAARDAMGKLRV
                     GAAVGVGDGTEERAELLVRAGVDVIVVDTAHGHSAGVIERVRWVKQNFPQVDVIGGNI
                     ATGAAARALAEAGADAVKVGIGPGSICTTRIVTGVGVPQIYAIQQVAKALEGSGVPLI
                     GDGGVRFSGDLAKAIAAGADTVMMGGAFAGTEEAPGETILYQGRTYKSYRGMGSIGAM
                     QQGSADRYFQDSSGSNNPNTTKLVPEGIEGQVPYKGPVVAVIFQMAGGLRASMHYCGC
                     ASIADMHEKAEF"
     gene            1512..2018
                     /locus_tag="VFA35_06730"
     CDS             1512..2018
                     /locus_tag="VFA35_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZT55902.1"
                     /translation="MSAMNRWKIVTLAAFLGLTLALPASAQWKWRDKTGQTQYSDLPP
                     PHNVADSDILQRPNANSRRAAAAASAASAASAAPPPAARASEPELEAKRKKAEQEAAD
                     KKKAEEAKLAATRADNCVRAKDYLRTIDSGQRIARVNAKGEREILDDATRAAEAKRAR
                     DVMASDCK"
     gene            complement(2043..2369)
                     /locus_tag="VFA35_06735"
     CDS             complement(2043..2369)
                     /locus_tag="VFA35_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HZT55903.1"
                     /translation="MAPDDRVLNVSVAYSPRAGEVDEVTLQLAPGATVADALRASGLQ
                     LRYPGLALDALPVGIWGAFCDRGDLLRDRDRVELYRPLRVDPKEARRQRHLAQARVKP
                     SPKVRR"
     gene            complement(2356..>2502)
                     /locus_tag="VFA35_06740"
     CDS             complement(2356..>2502)
                     /locus_tag="VFA35_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-binding protein"
                     /protein_id="HZT55904.1"
                     /translation="KACRIEFDLRYSFANGALDALISPVFDRIANTFVDSFVKRAEQV
                     YGAR"
BASE COUNT          404 a          897 c          838 g          363 t
ORIGIN      
        1 cgaactcggc cttctcgtgc atgtcggcga tgctggcgca gccgcagtag tgcatcgagg
       61 cgcgcaggcc gccggccatc tggaagatca ccgcgacgac cgggcccttg tacggcacct
      121 gcccttcgat gccttcgggc accagcttgg tggtgttcgg gttgttgctg cccgagctgt
      181 cctggaagta gcggtccgcc gagccctgct gcatcgcgcc gatcgagccc atgccgcggt
      241 agctcttgta ggtgcgcccc tggtacagga tcgtctcgcc cggcgcctct tcggtgccgg
      301 cgaacgcgcc gcccatcatc acggtgtcgg ccccggcggc gatcgccttg gccagatcgc
      361 ccgagaagcg cacgccgccg tcgccgatca gcggcacgcc gctgccctcg agcgccttcg
      421 cgacctgctg gatcgcatag atctgcggca cgccgacgcc ggtgacgatg cgcgtcgtgc
      481 agatcgaacc cgggccgatg ccgaccttga ccgcatcggc gccggcctcg gccagcgcac
      541 gcgcggccgc gccggtggcg atgttgccgc cgatcacgtc gacctgcggg aagttctgct
      601 tgacccaacg cacgcgctcg atcacgccgg cgctgtggcc gtgcgcggtg tcgacgacga
      661 tcacgtcgac gccggcccgc acgagcagtt cggcgcgctc ctcggtgccg tcgccgaccc
      721 ccacggccgc gccgacgcgc agcttgccca tcgcgtcgcg ggcggcgttc gggaagctgg
      781 tctgcttggt gatgtccttc accgtcatca ggccgcgcag ttcgaagtcg cggttgacga
      841 ccagcacgcg ctcgagcttg tgcttgtgca tcagcgcctt gccgtcctcg atcgtcgccg
      901 attcgggcac cgtgacgagg cgctcgcgcg gcgtcatgat ctcgctcacc ggggcgtcga
      961 gccgcgattc gaagcgcagg tcgcgattcg tgacgatgcc caccaccgtc gtgccctgca
     1021 gcaccgggaa gccggagacg ccgcgctggc gcgaaagctc catcaccgcg cgcaccggtg
     1081 tgtccggcgt caccgtgatc gggtcgcgca gcacacccga ctcgtagcgc ttgacgcgcg
     1141 ccacctcggc cgcctgctgc tgcggcgtca ggttcttgtg gacgatgccg ataccgcctt
     1201 cttgcgccag cgcgatcgcc aggcgggctt ccgtcaccgt gtccatggcg gcggacacca
     1261 ggggcaggtt caggcggatg ttgcgggaaa ggggggtcga cagatcggtg tcgcgaggca
     1321 acacctggga gaacgccggc accaacaata catcgtcgaa ggtgagcgct ttgccaagaa
     1381 ggcgcatggg gaaaagctcc agatgcaaaa gcgaattata cggatgcgcg cagaaccaca
     1441 cggatgcggg gtccccggtt ggcccgttcg acccccgccg catacactcc aaagccatga
     1501 caagttcacg aatgagcgcc atgaatcgtt ggaagatcgt caccttggcg gcatttctcg
     1561 gtttgacgct ggcgctgccg gcttcggccc agtggaagtg gcgcgacaag accggccaga
     1621 cgcagtacag cgacctgccg ccgccgcaca acgtcgccga cagcgacatc ctgcagcggc
     1681 ccaacgccaa cagccgccgc gccgcggcgg cggcctcggc cgcgtcggcc gcctcggccg
     1741 cgccgccgcc ggccgcgcgc gcttccgagc ccgaactcga agccaagcgc aagaaggccg
     1801 agcaggaggc cgccgacaag aagaaggccg aagaggccaa gctcgccgcc acccgggccg
     1861 acaactgcgt ccgcgcgaag gactacctgc gcacgatcga cagcggtcag cgcatcgcgc
     1921 gcgtcaacgc caagggcgaa cgcgagatcc tcgacgacgc gacccgcgct gccgaggcca
     1981 agcgcgcgcg cgacgtgatg gcgtcggact gcaagtgagc cggtgagccg ccgctcggtc
     2041 gcctagcgcc gcaccttcgg gctcggcttg accctggcct gcgccaggtg gcgctggcgt
     2101 cgcgcttcct tcgggtcgac gcgcaacggg cgatacaact cgacccgatc gcggtcgcgc
     2161 aacagatcgc cgcgatcgca gaacgcaccc cagatgccga ccggcagcgc gtccagcgcc
     2221 aagcccgggt agcgcagctg cagcccgctc gcgcgcagcg cgtcggcgac ggtcgcaccg
     2281 ggggcaagct gcagcgtcac ctcgtcgacc tcgcccgcgc gcgggctgta ggccacgcta
     2341 acgttcagga cacggtcatc gggcgccata cacctgctcg gcacgcttca cgaacgaatc
     2401 gacgaaggtg ttcgcgatgc ggtcgaacac cgggctgatc agcgcatcga gcgcgccgtt
     2461 ggcaaagctg tagcgcaggt cgaactcgat ccggcaggcc tt
//
LOCUS       DASRDG010000408         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1667937, whole genome shotgun sequence.
ACCESSION   DASRDG010000408 DASRDG010000000
VERSION     DASRDG010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667937"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFA35_06745"
     CDS             complement(<1..937)
                     /locus_tag="VFA35_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZT55905.1"
                     /translation="MTSKVTISRLKTGVPGLDDILGGGLPEFSFNLIAGQPGCGKTTL
                     AHQIMFALATPERPAIYFTVLGEPPLKMLRYQQQFAFFATEAINHTVRFINLSEETMA
                     GDLDRVLARIVQEVTTHSPALVFVDSFRSVALASESNANAHNGLQQFVQQLGMLMTSW
                     QATTFLIGEYFTESDANPVFTVCDGLIWMRQSVQRNSMVRKMEIMKMRGQPTLPGLHT
                     FRIGTSGIEVFPPASVALRSDAAANPPALSARLSVGVAGLDEMLGGGLPRGYSLLVAG
                     PSGSGKSILAAAFLAEGARAGEMGVIAAFEQHPNRS"
     gene            complement(934..1362)
                     /locus_tag="VFA35_06750"
     CDS             complement(934..1362)
                     /locus_tag="VFA35_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55906.1"
                     /translation="MQSQERNPISAFLEGKVAAGASSQDIAESVAAAFDALDRALTPI
                     VGQRGVAALYERSVHLARKAHPWLPVPQGSVPSAIDLRALVLGLAQRTAADAGTGGAA
                     LLQTFHDLLTHLIGASLTERLLRSVWANFLSGPSAGDTTR"
     gene            1544..2269
                     /locus_tag="VFA35_06755"
     CDS             1544..2269
                     /locus_tag="VFA35_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZT55907.1"
                     /translation="MREMMETNARENRLLAALPDDAWRRWSPLLEAVEMPLGLVLYES
                     GQRLSHVYFPTTAIVSLLYVMENGASAEIAVVGCEGLVGISIFMGGETTPSRAVVQSA
                     GTGFRLDALALKREFDRAPVMHLPLRYTQALITQMAQTAACNRHHTLDQQLCRWLLLS
                     RDRLRGDELLMTQELIANMLGVRREGVTAAALQLQQAGLIRYTRGHITLLDRGGLERR
                     CCECYAVVKKEYERLLPAGEASA"
     gene            complement(2288..2443)
                     /locus_tag="VFA35_06760"
     CDS             complement(2288..2443)
                     /locus_tag="VFA35_06760"
                     /inference="COORDINATES: protein motif:HMM:NF028510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HZT55908.1"
                     /translation="MEALRARDSISLGEGALYSMSVDEEPYITGYEPIKDASGKVIGA
                     YYVGYKK"
BASE COUNT          394 a          788 c          862 g          399 t
ORIGIN      
        1 gcgagcggtt cggatgctgc tcgaacgcgg cgatgacgcc catttcaccg gcgcgcgcgc
       61 cctcggcgag gaacgccgcg gccaggatgc tcttgcccga gccggacggc ccggcgacga
      121 gcagcgaata gccgcgcggc aggccgccgc ccagcatctc gtcgagcccc gccacgccga
      181 ccgacagccg cgccgacagt gcgggcgggt tcgcggccgc gtcgctgcgc agcgccacgc
      241 tggcgggcgg aaagacctcg atgcccgatg tgccgatgcg aaaggtatgc aggcccggca
      301 gggtcggctg gccgcgcatc ttcatgatct ccatcttgcg caccatggag ttgcgctgca
      361 cgctctggcg catccagatc aggccgtcgc agaccgtgaa caccgggttg gcgtcgctct
      421 cggtgaagta ttcgccgatc aggaaggtcg tcgcctgcca gctcgtcatc agcatgccga
      481 gttgctgcac gaactgctgc aagccgttgt gcgcattcgc gttgctctcg ctggccagcg
      541 ccaccgagcg gaacgaatcg acgaacacga gcgccgggct gtgggtggtt acctcctgga
      601 cgatgcgcgc gagcacgcgg tcgagatcgc cggccatcgt ttcttcggac aggttgatga
      661 aacgaacggt gtggttgatc gcctcggtgg cgaagaaggc gaactgctgc tggtagcgca
      721 gcatcttcag cggcggctcg ccgagcacgg tgaaataaat cgccgggcgt tccggggtcg
      781 ccagcgcgaa catgatctgg tgcgccagcg tcgtcttgcc gcagccgggc tggccggcga
      841 tcaggttgaa cgagaactcc ggcaagcccc cgccgaggat gtcgtccagg cccggaacgc
      901 cggtcttcag gcggctgatg gttactttgc tggtcatcgc gtggtgtccc cggcggacgg
      961 accgcttaag aagttggccc agaccgaccg caacaggcgt tcggtgagcg aagcgccgat
     1021 caagtgggtc aacaggtcat ggaaggtctg cagcagcgcg gcaccgccgg tgccggcatc
     1081 ggcagccgtg cgctgcgcca gccccaggac cagtgcgcgg aggtcgatcg cgctcggaac
     1141 gctcccttgc ggaaccggca accagggatg ggccttcctg gcgagatgca cgctacgctc
     1201 gtagagtgcg gccacgccgc gctggccgac gatgggcgtc agtgcgcggt cgagggcatc
     1261 gaatgccgcg gcgaccgact cggcgatgtc ttgcgacgac gcaccggcgg ccaccttgcc
     1321 ttcgagaaag gcgctgatcg ggttgcgctc ctggctttgc atcgatggga tgccggattc
     1381 ggcggcctcg ttgggtggat ggtagcggcg ttcgcgatca ccggcaggtc ggccgtgaac
     1441 gccgggaccg gcaacggtaa agtagcaggg cgttgggctc gggtctgtgc gctggcgcac
     1501 gttcgatgct gccgccgccg gccggcacgg aggttcctga acgatgcggg agatgatgga
     1561 gacgaacgcc agagagaatc gcttgctggc ggcattgccc gacgacgcat ggcggcgctg
     1621 gtcgccgttg ctcgaagcgg tcgagatgcc gttgggcctc gtgctgtacg aatcggggca
     1681 aaggctcagc cacgtctact ttccgaccac cgcgatcgtc tcgctgttgt acgtgatgga
     1741 gaacggcgcc tcggccgaga tcgccgtggt cggctgcgaa ggcctcgtcg gcatctcgat
     1801 cttcatgggc ggcgagacca cgccgagccg ggcggtggtg cagagcgcgg gcaccggttt
     1861 tcgcctcgac gcgctggcgc tcaagcgcga gttcgaccgc gcgccggtga tgcacctgcc
     1921 gctgcgctac acccaggcac tgatcaccca gatggcgcag accgcggcgt gcaaccggca
     1981 tcacacgctc gatcagcagc tgtgccgctg gctgctgctg agccgggacc gcctgcgcgg
     2041 cgacgaactc ctgatgaccc aggagttgat cgccaacatg ctgggcgtgc ggcgcgaggg
     2101 cgtcaccgcg gccgcgctgc agctgcagca ggccgggctg atcaggtaca cgcgcggcca
     2161 catcaccctg ctcgaccgcg gcggcctgga gcggcgctgc tgcgagtgct atgccgtggt
     2221 gaagaaggag tacgagcggc tgcttccggc cggcgaagcg agtgcatgac ccgggcctcg
     2281 ccgcgagcta tttcttgtag ccgacgtaat aggcaccgat caccttgccc gacgcgtcct
     2341 tgatgggctc atagccggtg atgtacggct cctcatcgac gctcatgctg taaagcgcgc
     2401 cttcgccgag gctgatggaa tccctcgccc gcagagcttc cac
//
LOCUS       DASRDG010000409         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1573861, whole genome shotgun sequence.
ACCESSION   DASRDG010000409 DASRDG010000000
VERSION     DASRDG010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573861"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFA35_06765"
     CDS             complement(<1..1081)
                     /locus_tag="VFA35_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55909.1"
                     /translation="MVRGLLPFLPLLLLAAAPLRAQDGESDYGVMLQDAQRKLLKGQL
                     RAAEARFQEIIEPGEDEKEKALVPAAVRIGAQAGLLEIDQRRGAYEKVIDGTQALAAA
                     AGGTPPALAATLLELRARALLAVGRHRDAIDLLRARVAADANDFEAHWRLGEALWADG
                     QRAAARAAWAAAVELPQPDDGAQLAFRARCRWRLGARDQLEAASEELVRSMKLAPERP
                     EARTTYGILWFERGGEAGGFKSGESELKKVLDQNGDYEEALLAMYRSRSANYTLDAGK
                     TETFLERVLHQNPRCVPAIVLRGSAVLDDRRYKDAAELLDEALQIDGEDRIALAHRAA
                     AAWLLHDEPGYAAFRARALAGDPRWP"
     gene            complement(1240..2592)
                     /locus_tag="VFA35_06770"
     CDS             complement(1240..2592)
                     /locus_tag="VFA35_06770"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZT55910.1"
                     /translation="MNDPVLVVDDDDALREALVAALEPLPVEITAASSGREAVDLLRE
                     RRFAVTVTDLVMKDVDGFAVLKASKDGYPNGRVVMLTGHGSRDVAVQAMEQGATYYVE
                     KPVDLNDLRAKVRKCLEDHQKDLAYDDLKGQLDRTFGIQGIVGQDPKMQRVLDMVRQV
                     APTNASVLILGASGTGKELVARAIHSLSPRSKRPFVPINCGGMSEGTIESELFGHVKG
                     AFTGAVADREGKFEYSNGGTLLLDEVGEMPPQTQVKLLRVLEERTVARLGDNRPRPVD
                     VRVLAATNADLQQRVKSGEFRQDLYFRLRVVTIELPALKDRRGDIKLLVDHYLRHFAK
                     LHGKDVESIDREALVALVQYDWPGNVRELKNSIESMVVRTRGNILTRSDLPPEIHAPL
                     PGDQDSWQFLAGRSVDEVEKNHIRVSLELSGGNRQKAAKSMGLSERTLYRKLKQYGLE
                     "
     gene            complement(2589..2717)
                     /locus_tag="VFA35_06775"
     CDS             complement(2589..2717)
                     /locus_tag="VFA35_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55911.1"
                     /translation="MRHVVEEHGGSVAFTSEPGVGTQFTVRLPGPAQNGGERGASA"
BASE COUNT          338 a         1026 c          954 g          413 t
ORIGIN      
        1 cgggccagcg cggatcgccc gcgagcgcgc gggcgcggaa cgccgcgtag cccggctcgt
       61 cgtgcagcag ccacgccgcc gccgcgcggt gcgcgagcgc gatgcggtcc tcgccgtcga
      121 tctgcagcgc ttcgtcgagc agctcggcgg cgtccttgta gcgccggtcg tcgagcaccg
      181 cgctgccgcg cagcacgatc gccggcacgc agcgcggatt ctggtgcagc acgcgctcga
      241 ggaaggtctc ggtcttgccg gcgtcgagcg tgtagttcgc gctgcgggac cggtacatcg
      301 ccagcagcgc ctcctcgtag tcgccgttct ggtcgagtac tttcttgagt tcgctctcgc
      361 cgctcttgaa gcctcccgcc tcgccgccgc gctcgaacca caggatgccg taggtcgtgc
      421 gcgcctccgg ccgctcgggc gccagcttca tcgagcgcac cagctcctcc gacgcggctt
      481 cgagctggtc gcgggcgccg agccgccagc ggcagcgggc gcggaacgcg agctgcgcgc
      541 cgtcgtccgg ttgcggcagc tccaccgccg cggcccacgc cgcgcgcgcg gccgcgcgct
      601 ggccgtccgc ccacagcgcc tcgccgagcc gccagtgcgc ctcgaagtcg ttcgcgtcgg
      661 cggcgacgcg ggcgcgcagc agatcgatcg cgtcgcggtg gcggccgacg gcgagcagcg
      721 cacgcgcgcg cagttcgagc agcgtggccg cgagcgccgg cggcgtcccg ccggccgcgg
      781 ccgcgagcgc ctgcgtgccg tcgatgacct tctcgtaggc gccgcgacgc tggtcgatct
      841 cgagcaggcc cgcctgcgcg ccgatgcgca ccgccgcggg caccagcgcc ttctccttct
      901 cgtcctcgcc gggctcgatg atctcctgga acctggcctc ggcggcgcgg agctggccct
      961 tcagcagctt gcgctgcgca tcctgcagca tcaccccgta gtcggactcg ccgtcctgcg
     1021 cgcgcaacgg cgcggcggcg agcagcagca gcggcaggaa cggaaggagt ccgcggacca
     1081 tgctcctcca ccatacggca acggcgggcg cgttgctgga gtggaaacgg tcgggccgcg
     1141 ccggctgcag gccgcggccg ggcgaagcgc cgaaccgccg ccagcaccgc agcatgccga
     1201 cggcgccgag cggccggaac ccggggccgc ggcgggccgc tattcgagcc cgtactgctt
     1261 cagcttccgg tacagcgtcc gctcgctgag tcccatcgac ttcgccgcct tctggcggtt
     1321 gccgccggac agctcgagcg acacgcggat gtggttcttc tcgacctcgt cgacgctgcg
     1381 gccggcgagg aactgccagc tgtcctggtc gcccggcagc ggcgcgtgga tctcgggcgg
     1441 cagatcgctg cgcgtgagga tgttgccgcg cgtgcgcacg accatcgact cgatcgagtt
     1501 cttgagctcg cgcacgttgc cgggccagtc gtactgcacg agcgcgacga gcgcttcgcg
     1561 gtcgatcgac tcgacgtcct tgccgtgcag cttcgcgaag tgcctgaggt agtggtcgac
     1621 gagcagcttg atgtcgccgc ggcggtcctt cagcgccggc agctcgatgg tgaccacgcg
     1681 caggcggaag tagaggtcct gccggaactc gcccgacttg acccgctgct gcaggtcggc
     1741 gttggtcgcc gcgagcacgc gcacgtcgac gggccgcggc cggttgtcgc ccagccgcgc
     1801 gacggtgcgc tcctcgagca cccgaagcag cttcacctgc gtctgcggcg gcatctcgcc
     1861 gacctcgtcg agcagcagcg tgccgccgtt gctgtactcg aacttgccct cgcggtcggc
     1921 gaccgcgccg gtgaacgcgc ccttcacgtg gccgaacagc tcgctctcga tcgtgccctc
     1981 cgacatgccg ccgcagttga tcggcacgaa cggccgcttg ctgcgcggcg acagcgagtg
     2041 gatcgcgcgc gcgaccagct ccttgcccgt gcccgacgcg ccgaggatca gcaccgacgc
     2101 gttggtcggc gcgacctggc gcaccatgtc gagcacgcgc tgcatcttcg ggtcctggcc
     2161 gacgatcccc tggatgccga acgtgcggtc gagctggccc ttgaggtcgt cgtaggcgag
     2221 gtccttctgg tggtcctcga ggcacttcct gaccttcgcg cgcaggtcgt tcaggtcgac
     2281 cggcttctcg acgtagtacg tcgcgccctg ctccatcgcc tgcacggcga cgtcgcggct
     2341 gccgtggccg gtgagcatca cgacgcgccc gttcgggtag ccgtccttgc tcgccttcag
     2401 taccgcgaag ccgtcgacgt ccttcatcac caggtcggtc accgtgaccg cgaaccgccg
     2461 ttcgcgcagc agatcgaccg cctcccggcc cgacgacgcg gcggtgatct cgaccggcag
     2521 cggttcgagc gcggcgacca gcgcctcgcg cagcgcgtcg tcgtcgtcga cgacgaggac
     2581 cggatcgttc atgccgaggc cccccgctcg ccgccgttct gcgcgggccc gggcagccgc
     2641 accgtgaact gcgtgcccac gccgggctcg ctcgtgaacg cgaccgaacc gccgtgctcc
     2701 tcgaccacgt ggcgcacggt cggcaggccg a
//
LOCUS       DASRDG010000410         5081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1573930, whole genome shotgun sequence.
ACCESSION   DASRDG010000410 DASRDG010000000
VERSION     DASRDG010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573930"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="VFA35_06780"
     CDS             <1..1630
                     /locus_tag="VFA35_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55912.1"
                     /translation="IRQACRAAVPEALRAWLHGRIAAALEAQHAAPGRIAPHARAAGE
                     LRSAGTQFRAAADAARAAGRLSEEVRLLATAAECFDELGDRPARFESLARRAMAALEA
                     LPPAQAMEAAEALVDAAAGDRELGIALRLVGACHLNGARFERAAPELERAIECSERGG
                     DTDSSCHARYLLALTVVQTQGAAPALERIESLLAWAESQPDIGLRHAFIADLAILLDQ
                     ADQRRRARPHFERALAYFDAVRAVGDAASTRMMFARSLGLLGDLAQACALLEVAERER
                     EALSEGAGGQGIEALNLGRAYCELGRYDEALRLLEPTRQRLAEGGTSVVGTAVALVMA
                     RVLLHLGQGARAQALCAEPGADAPFHQRATWLWTCALLALERPAERTRLLGAAEREFE
                     RTDLPFVRLPIQFDRLGSEAADDAAVASCRLLINECERRELPAPQMLGRARLAQLLLA
                     QRELDTALTVVRGLQADLASCHPVGMYLPELHWICHRVAHAAGDSAFAAHCLDAAVRW
                     IRDTALPHVPAPYRDSFVRRQPINRRILAAASLL"
     gene            1720..1986
                     /locus_tag="VFA35_06785"
     CDS             1720..1986
                     /locus_tag="VFA35_06785"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HZT55913.1"
                     /translation="MHPAGRGILLSAATVALAGVGFALLWPRRAADGLYYLDHRAQPR
                     GPIDRGRLRAMLADGRLAPDAPVSLDGSDDWRAAIHWIGRGRRA"
     gene            1983..2822
                     /locus_tag="VFA35_06790"
     CDS             1983..2822
                     /locus_tag="VFA35_06790"
                     /inference="COORDINATES: protein motif:HMM:NF020395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family c-type lysozyme inhibitor"
                     /protein_id="HZT55914.1"
                     /translation="MNRENDMGFRSLRVALLMIAVGYAAVPACAADFGADALRRYGGT
                     YSTRCDDAAAPTARIEATALRLQANGRSHVGSGVQDAVSYFGQAAPPNYQTALLSTLP
                     GGRELNFIVYRDGRGRYLTVAADPKLLAALGVPARATLTLRDCDTGRARADGAAARAE
                     LAQEARDAREAERSSPVDARFRALYRQALGVKRHEPWLVEMDGPSHGPKTVQIGAVSF
                     VRYGFCKPHDCADNRVLLLYSAERRILVGRLQEHGSQFFVFGAPSPAMAAELRRQWDA
                     EWR"
     gene            2840..3895
                     /locus_tag="VFA35_06795"
     CDS             2840..3895
                     /locus_tag="VFA35_06795"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HZT55915.1"
                     /translation="MNKSRIVGGFWVVAALAGSAPAAHALDPEAARLNPMTPYERSMQ
                     QQQLNDQQQQNARQQQQLQQQRQDQVYDDALRQQQARQRGDIAQGQAVLRSWQQRPPL
                     AADKNPLLGRWNSLGSAGAKRSAPGVSPEMAQLVNSMLGGITGGMCDSMLGRGLVEFR
                     PNAVVAIDGSGRERALYRAEYRGGGARVVVLPQGGTSFTHMIIDFNGRNQASVAAVGC
                     GLTRVGGVAAAESAAATATPVQWDLLGSSQANGGMDVYVARSTIRKTGHAAQMADLYD
                     FKTVQVFEGKRYLSARNQWEYDCTRARVRMVGTVGYAGHMGQGAVIASDGETLVWESV
                     PPSGPLRDIWKIACAKA"
     gene            complement(3918..4565)
                     /gene="ureG"
                     /locus_tag="VFA35_06800"
     CDS             complement(3918..4565)
                     /gene="ureG"
                     /locus_tag="VFA35_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HZT55916.1"
                     /translation="MNTALHRIANRTRKLPPLRVGVGGPVGSGKTTLVEMLCKAMRAT
                     YDLVVVTNDIYTKEDQRLLTVAGALDAERIMGVETGGCPHTAIREDASINLEAVDRML
                     EKFPDADIVFIESGGDNLAATFSPELSDLTIYVIDVAAGEKIPRKGGPGITKSDLFVI
                     NKTDLAPHVGANLDVMAADTKRMRGARPFVMSNLKTNQGLAEVVRFIETKGMLAA"
     gene            complement(4590..>5081)
                     /locus_tag="VFA35_06805"
     CDS             complement(4590..>5081)
                     /locus_tag="VFA35_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HZT55917.1"
                     /translation="ERVDHIEDELLTSRLDHKRAGLGALRRLLVRLQRLLAPEPAALF
                     RLLQHPPGWMTDVDVQELRQASEEFSVVLHDMASLQERIKLLQEEIAAQVNEGNNRSL
                     FVLTIVTVLALPINIIAGLLGMNVGGVPLAQHAHGFWIVVAVVASFTAIAGWVAFRKQ
                     RGA"
BASE COUNT          697 a         1824 c         1830 g          730 t
ORIGIN      
        1 gatccggcag gcgtgccgcg ccgccgtgcc cgaggcgctg agggcctggc tgcacggccg
       61 catcgccgcg gcgctcgaag cccagcacgc ggcgccgggg cggatcgcgc cgcacgcccg
      121 tgcggccggc gaactgcgca gcgccggcac gcagttccgc gccgcggccg acgccgcgcg
      181 cgcggccggc cgcctgtccg aagaggtgcg cctgctcgcc actgccgccg aatgcttcga
      241 cgaactcggc gaccggccag cgcgcttcga gagcctggcc cggcgcgcga tggcggccct
      301 cgaagccttg ccgccggcgc aggcgatgga ggcggccgag gcgctcgtcg acgccgctgc
      361 cggcgaccgc gaactcggca tcgcgctgcg cctggtcggc gcctgccacc tgaacggcgc
      421 gcgcttcgag cgcgccgcgc ccgagctcga gcgcgcgatc gaatgctcgg agcgcggcgg
      481 cgacaccgac agctcctgcc acgcgcgcta cctgctggcc ttgaccgtcg tgcagaccca
      541 gggcgcggcg ccggcgctcg aacgcatcga gtcgctgctc gcatgggccg agtcgcaacc
      601 cgacatcggc ctgcgccacg ccttcatcgc cgacctggcg atcctgctcg accaggccga
      661 tcaacgccgg cgcgcgcggc cgcacttcga acgcgcgctg gcgtatttcg acgcggtgcg
      721 tgcggtcggc gacgcggcga gcacgcgcat gatgttcgcg cgcagcctgg gcctgctggg
      781 cgacctggcg caggcctgcg cgctgctcga agtcgccgag cgcgagcgcg aggcgctctc
      841 cgaaggcgcc ggcggccagg gcatcgaggc gctcaacctc ggccgtgcct actgcgagct
      901 cggccgctac gacgaagcgc tgcgtctgct cgaaccgacg cgccagcgcc tggccgaggg
      961 cggcaccagc gtcgtcggca ccgcggtcgc gctggtgatg gcgcgcgtgc tgctgcacct
     1021 ggggcagggc gcgcgcgcgc aggcactgtg cgccgagccg ggcgccgatg cgccgttcca
     1081 ccagcgtgcc acctggctct ggacctgcgc gctgctcgcg ctcgaacgcc cggccgagcg
     1141 cacgcgcctg ctcggcgccg ccgagcgcga gttcgaacgc accgacctgc cgttcgtgcg
     1201 cctgccgatc cagttcgatc ggctcggcag cgaggccgcc gacgatgcgg cggtcgcgag
     1261 ctgccgcctg ctgatcaacg agtgcgagcg ccgcgaactg ccggcgccgc agatgctcgg
     1321 gcgggcgcgg ctcgcccagc tgctgctcgc gcagcgcgaa ctcgacaccg cgctgaccgt
     1381 cgtgcgcggg ctgcaggccg atctggcgtc gtgccatccg gtcggcatgt acctcccgga
     1441 gctgcactgg atctgccatc gcgtcgcaca cgcggccggc gactcggcct tcgccgcgca
     1501 ttgcctggac gccgcggtgc gctggatccg cgacaccgcg ctgccccatg tgcccgcgcc
     1561 gtaccgcgac agcttcgtcc ggcgccagcc gatcaaccga cgcatcctcg cggcggcctc
     1621 gctgctgtag taacgggtcg gtaacggcct gcgcctaggc ttgcaatgtg cgacaggcgg
     1681 tgcgtccggc ctgtcgtcgc aagggcagag gaggcgagca tgcatccagc gggccgggga
     1741 atcttgctgt cggcggccac ggtcgcgctg gccggtgtcg gcttcgcgct gctgtggccg
     1801 cgccgcgccg ccgatggcct gtactacctc gaccaccgcg cgcagccgcg cgggccgatc
     1861 gaccgcggcc gcctgcgcgc gatgctcgcc gacggccggc tcgcgccgga tgcgccggtc
     1921 tcgctcgacg gttcggacga ctggcgcgcg gcgatccact ggatcgggcg cgggcgccgc
     1981 gcgtgaacag ggagaacgac atgggctttc gctcgctccg ggttgcgttg ctgatgatcg
     2041 ccgtcgggta cgccgccgtg ccggcatgcg ctgccgactt cggcgccgat gcgttgcgcc
     2101 gctacggcgg cacctactcg acacgctgcg acgatgccgc tgcgccgacc gcgcgcatcg
     2161 aagccacggc gctacgcctg caggccaacg gccggagcca cgtcggcagc ggggttcagg
     2221 acgcggtctc gtacttcggt caggccgcgc cgccgaacta ccagactgcg ttgctgagca
     2281 cactgcccgg cggtcgcgaa ctgaacttca tcgtctaccg cgacggtcgc gggcgctacc
     2341 tcacggtcgc ggccgatccg aagctgctgg ccgcgctcgg cgtgccggct cgcgccacct
     2401 tgacgctgcg cgactgcgac acggggcggg cccgggccga cggcgccgcg gcgcgcgccg
     2461 agctcgctca ggaggcgcgc gatgcgcgcg aggccgagcg cagctcgccg gtcgatgcca
     2521 ggtttcgcgc gctctatcgc caggcgctcg gcgtgaagcg gcacgagccc tggctggtcg
     2581 agatggacgg ccccagccat gggccgaaga cggtgcagat cggcgccgtc tcgttcgtgc
     2641 gctacgggtt ctgcaagccg cacgactgcg ccgacaaccg cgtgctgctg ctgtattcgg
     2701 ccgaacgccg catcctcgtc ggaagactcc aggagcacgg ttcgcagttc ttcgtgttcg
     2761 gtgcaccgtc acccgcgatg gcggccgaac tgcggcggca atgggacgcc gagtggcgtt
     2821 gattcgaaag gagagcgtga tgaacaagtc gcgaatcgtc ggaggcttct gggtcgtcgc
     2881 ggcgctcgcc ggcagtgcgc cggcggcgca cgcgctcgac ccggaggcgg cgcgcctgaa
     2941 cccgatgacg ccttacgagc gcagcatgca gcaacagcaa ttgaacgacc agcagcagca
     3001 gaacgcccgc caacagcagc aactgcaaca gcagcggcaa gaccaggtct acgacgacgc
     3061 cctccgccag cagcaggcgc gccagcgcgg cgacatcgcg caaggccagg cggtgctgcg
     3121 cagctggcag cagcgcccgc cgctggcggc cgacaagaac ccgctgctcg ggcgctggaa
     3181 ttcgctcggc tcggccggtg cgaagcgctc ggcccccggc gtcagcccgg agatggcgca
     3241 actcgtcaac tcgatgctcg gcggcatcac cggcggcatg tgcgattcga tgctcggccg
     3301 cggcttggtc gagttcaggc cgaacgcggt ggtcgcgatc gacggcagcg gccgcgagcg
     3361 cgcgctgtac cgcgccgagt accgcggcgg gggtgcgcgc gtcgtcgtgc tgccgcaggg
     3421 cggcacctcg ttcacccaca tgatcatcga cttcaacggg cgcaaccagg cgagcgtcgc
     3481 ggcggtcggc tgcggcctga cgcgcgtcgg cggcgttgcc gcggccgaga gcgccgcggc
     3541 cactgcgaca ccggtgcagt gggacctgct cggcagttcg caggcgaacg gcgggatgga
     3601 cgtctacgtc gcgcgctcga cgatccgcaa gaccggccac gcggcgcaga tggcggacct
     3661 gtacgacttc aagacggtgc aggtgttcga aggcaagcgc tacctgtcgg cgcgcaacca
     3721 gtgggagtac gactgcacgc gcgcgcgtgt gcggatggtc ggaacggtcg gctatgcggg
     3781 gcacatgggc caaggcgcgg tgatcgcctc cgacggcgaa acgcttgtct gggagagcgt
     3841 cccgccgagc ggcccgctgc gggacatctg gaagatcgcc tgcgccaagg cctgacgcgc
     3901 tcgcgtccgc cgctggctca ggccgcgagc atgcccttgg tctcgatgaa gcgcacgacc
     3961 tcggccagac cctggttcgt cttcaggttg ctcatcacga acgggcgggc gccgcgcatg
     4021 cgcttcgtgt cggcggccat cacgtcgagg ttcgcgccga catggggcgc gagatcggtc
     4081 ttgttgatca cgaacaggtc gctcttcgtg atgccggggc cgcccttgcg cgggatcttc
     4141 tcgccggccg cgacgtcgat cacgtagatc gtcaggtcgc tgagctcggg gctgaaggtc
     4201 gccgcgaggt tgtcgccgcc gctctcgatg aagacgatgt cggcgtccgg aaacttctcg
     4261 agcatgcggt cgaccgcttc gaggttgatc gacgcgtcct cgcggatcgc ggtgtgcggg
     4321 cagcccccgg tttcgacgcc catgatgcgt tcggcgtcga gcgcgccggc gaccgtcaac
     4381 agccgctggt cttccttggt gtagatgtcg ttcgtgacca cgaccaggtc gtaggtcgcg
     4441 cgcatcgcct tgcacagcat ctcgaccagc gtcgtcttgc ccgacccgac cggcccgccg
     4501 acgccgacgc gcagcggcgg gagtttgcgg gtgcggttcg cgattctgtg cagggcggtg
     4561 ttcatacggc cgattatggc ggcggcgcct caagcgccgc gctgcttgcg gaacgcgacc
     4621 cagccagcga tcgccgtgaa gctggcgacg accgcgacga cgatccagaa cccgtgcgca
     4681 tgctgcgcga gcggcacgcc gccgacgttc atgcccagca gcccggcgat gatgttgatc
     4741 ggcagcgcga gcaccgtcac gatcgtcagc acgaacaggc tgcggttgtt gccctcgttg
     4801 acctgcgcgg cgatctcttc ctgcagcagc ttgatccgct cctgcaggct cgccatgtcg
     4861 tgcagcacga ccgagaactc ctcgctcgcc tggcgcagtt cctgcacgtc gacgtcggtc
     4921 atccagcccg gcggatgctg cagcagccgg aacagcgccg cgggttcggg cgcgagcagg
     4981 cgctgcagcc gcaccagcag ccggcgcagt gcgccaaggc cggcgcgttt gtggtcgagg
     5041 cgactcgtca gcagttcgtc ctcgatgtgg tcgacgcgtt c
//
LOCUS       DASRDG010000411         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_866688, whole genome shotgun sequence.
ACCESSION   DASRDG010000411 DASRDG010000000
VERSION     DASRDG010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866688"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VFA35_06810"
     CDS             <1..824
                     /locus_tag="VFA35_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55918.1"
                     /translation="LLDELGAGTDPEEGGVLGYAVLEQLLAARAFAVVTTHLGRLKDF
                     AYQHQGAENGSMAFDGTTLAPLYRLDVGIPGQSHALDIAGRVGMPPSVVQRARELLGK
                     RDTSLQDVIERVQVARAGAEADRRRTAEVSRNVERQQTQLQEQLTEAKRKEGWLQEEA
                     DAVVEAELRRAQSALAEGLVPLLSAPGAHGERARVLKQIVDALQKRAGMHRRRMKFCN
                     ELKKGDMVFLPRWRRLCQVHKVDRVKESVVVDYGNVKMEVPFEDVSWLQPLDGGA"
     gene            901..>2085
                     /locus_tag="VFA35_06815"
     CDS             901..>2085
                     /locus_tag="VFA35_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55919.1"
                     /translation="MPGLRSTTLLFVLLLLAPAAILAFLGFRASSSLVQELRRAARDE
                     AVRAAQDAAAALPVALAAAAIDDGRRLAAVAAQVRERLPMATAGAVLRALDAPGGGAL
                     VVRGSDGRAIAPAGPLAPRADGLPEWPRFCAVRARCDRGERGAPVADAAAALATPALR
                     AAALALAWRDDAEAAAAALAPLPFPALAQAGPLAVGALARSTGGRPRLAAARADGALD
                     DVPATEAARLGWLESAGAGEDELARQRLRALAEAGGAVLAASAPVVPGVRVEEWVSPA
                     AWIDRCLPHDAGAPCALAAVRTDRLQPPRAPGPAAASVSVATPLGPVEVTATHRGIAA
                     IEAAARRERWLTGAGIAMLLLTMVAGVALVRRALLRERRAAQLRDDFIANVSHDLRTP
                     LTS"
BASE COUNT          229 a          777 c          837 g          242 t
ORIGIN      
        1 tcctgctcga cgagctcggc gccggcaccg accccgagga gggcggcgtg ctcggctacg
       61 cggtgctcga gcagctgctc gcggcgcgcg cgttcgcggt cgtcaccacg cacctcggcc
      121 ggctcaagga cttcgcctac cagcaccagg gcgccgagaa cggttcgatg gcgttcgacg
      181 gcaccacgct ggcgccgctc taccgcctcg acgtcggcat tcccggccag agccatgcgc
      241 tcgacatcgc cggtcgcgtc ggcatgccgc cgtcggtcgt gcagcgcgcg cgcgagctgc
      301 tcggcaagcg cgacacctcg ctgcaggacg tgatcgagcg cgtgcaggtc gcgcgcgccg
      361 gcgccgaggc cgaccggcgg cgcacggccg aggtgagccg caacgtcgag cgccagcaga
      421 cgcagctgca ggagcagctg acggaagcca agcgcaagga gggctggctg caggaggagg
      481 ccgacgcggt ggtggaggcc gagctgcgcc gggcgcagtc ggcgctcgcc gagggactgg
      541 tgccgctgct cagcgcgccc ggtgcacacg gcgaacgcgc gcgcgtgctc aagcagatcg
      601 tcgacgcgct gcagaagcgc gcgggcatgc accgccggcg catgaagttc tgcaacgagc
      661 tcaagaaggg cgacatggtg ttcctgccgc gctggcgccg gctgtgccag gtgcacaagg
      721 tcgaccgcgt caaggagtcg gtcgtcgtcg actacggcaa cgtgaagatg gaggtcccgt
      781 tcgaggacgt ctcctggctg cagccgctcg acggcggcgc gtgagcgccg cggaccgagg
      841 cgacgctcag accttgcgat tcgccaacaa ctcgccgccg gcggccgcca tgctggtgcc
      901 gtgcccggcc tgcggtcgac gacactgctg ttcgtgctgc tgctgctcgc gccggcggcg
      961 atcctcgcgt tcctcggctt ccgcgcctcg tcgtcgctgg tgcaggaact gcggcgcgcc
     1021 gcacgcgacg aggcggtgcg cgccgcacag gacgccgccg cggcgctgcc cgtggcgctc
     1081 gccgccgcgg cgatcgacga cggccggcgg ctcgcggcgg tggcagcgca ggtgcgggag
     1141 cgcctgccca tggcgacggc gggcgcagtg ctgcgcgcgc tcgacgcgcc aggcggcggc
     1201 gcgctcgtcg tgcgcggatc ggacggccgc gcgatcgcgc ccgccgggcc gctggcgccg
     1261 cgcgccgacg gcttgccgga gtggccgcgg ttctgcgccg tgcgcgcgcg ctgcgaccgc
     1321 ggcgaacgcg gcgcgccggt ggccgacgcc gcggcggccc tggcgacgcc ggcgctgcgc
     1381 gcggcggcgc tggcgctggc gtggcgcgac gacgccgagg ccgcggcggc ggcgctggcg
     1441 ccgctgccgt tccccgcgct cgcgcaggcc ggtccgttgg cggtcggggc gctggcgcgc
     1501 tcgaccggcg gccgaccgcg gctcgcggcg gcgcgcgcgg acggggcgct cgacgacgtg
     1561 cctgcgaccg aggccgcgcg cctcggctgg ctcgaatccg ccggcgcggg cgaggacgaa
     1621 ctcgcgcgcc agcgcctgcg cgcgctcgcc gaggctggtg gcgcggtgct ggcggcgagc
     1681 gccccggtcg tgccgggcgt gcgcgtggag gagtgggtct cgccggccgc ctggatcgac
     1741 cgctgcctgc cgcacgacgc cggcgcgccg tgcgcgctcg cggccgtgcg cacggaccgg
     1801 ctgcagccgc cgcgcgctcc cggccccgcg gcggcgagcg tgtcggtcgc gacgccgctg
     1861 gggccggtcg aggtcacggc gacgcaccgc ggcatcgccg cgatcgaggc cgcggcgcgg
     1921 cgcgagcggt ggctcaccgg cgccggcatc gcgatgctgc tgctgacgat ggtcgccggc
     1981 gtggcgctgg tgcggcgcgc gctcctgcgc gaacggcggg cagcgcagct gcgcgacgac
     2041 ttcatcgcca acgtctcgca cgacctgcgc acgccgctca cgagc
//
LOCUS       DASRDG010000412         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1323587, whole genome shotgun sequence.
ACCESSION   DASRDG010000412 DASRDG010000000
VERSION     DASRDG010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323587"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFA35_06820"
     CDS             <1..675
                     /locus_tag="VFA35_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZT55920.1"
                     /translation="HAAFGETNVAMNAYMVEHLGVDHDEAGRLRLQYLRRYGATLLGL
                     VRHHGVEAAHFLEQTHLHPGLEARLRMSRHDRAAVKRLRGRKYILTNAPRGYTMRVLN
                     TLRLADLFDGVISIEDMHMFGDLRPKPDARMFRHIAARLKVRASDCVLVEDVLQNQKA
                     ARGLGMRTAWMQRYLEGRYRGHLRGGAHPGSNAGFRRREVGVHPCPSPRYVCAKIRSL
                     QQLLTL"
     gene            675..1343
                     /gene="slmA"
                     /locus_tag="VFA35_06825"
     CDS             675..1343
                     /gene="slmA"
                     /locus_tag="VFA35_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HZT55921.1"
                     /translation="MDTNAEASAAENTDPIDAAALAEAPAPPVRKRPKPGERRVQILQ
                     TLASMLEQPGTDRITTAALAAELDVSEAALYRHFASKAQMFEGLIEFIEQTVFTLINQ
                     IHEREAASNLQVQKMLTVLLQFAEKNPGMARVMVGDALVFENERLLARMNQFFERFES
                     QLRQSLRGVAEAAGSATPTVDANVQASVLTAFAVGRLQRFARSGFKRTPAEHLDAALQ
                     MLAA"
     gene            1401..>2207
                     /locus_tag="VFA35_06830"
     CDS             1401..>2207
                     /locus_tag="VFA35_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT55922.1"
                     /translation="MPTPTDPLLHLIARAEALLGRLEAVLPHPPTAPDWNAAIAFRYR
                     KRGGSGVIEPVRHVAPIRLAALVEVEAQKERLLRNTQQFVDGLKANNVLLTGARGTGK
                     SSLIKACLNEFADRGLRLIEVDKTDLVDLPDIVDLVAERPERFIVFCDDLSFDEGEPG
                     YKALKSILDGSVAQSSDNVLIYATSNRRHLLPEYMKENLSYQHLENGEVHPGEVVEEK
                     ISLSERFGLWVSFYPFTQAEYLTIVAQWLRGCGVDEVAIAAARQESLVWAL"
BASE COUNT          351 a          791 c          739 g          326 t
ORIGIN      
        1 cacgcggcgt tcggcgagac caatgtcgcg atgaacgcct acatggtcga gcacctcggt
       61 gtcgaccacg acgaggcggg ccggctgcgc ctgcagtacc tgcgccgcta cggcgcgacg
      121 ctgctcggcc tggtgcgcca ccacggcgtc gaggccgcgc actttctcga gcagacccac
      181 ctgcatccgg gcctcgaagc gcgcctgcgc atgagccgcc acgaccgcgc ggcggtcaag
      241 cggctgcgcg gccgcaagta catcctgacc aacgccccgc gcggctacac gatgcgcgtg
      301 ctgaacacct tgcgcctggc cgacctgttc gacggcgtga tcagcatcga ggacatgcac
      361 atgttcggcg acctgcgccc gaagcccgac gcccggatgt tccgccacat cgccgcgcgg
      421 ctgaaggtgc gggccagcga ctgcgtgctg gtcgaagacg tgctgcagaa ccagaaggcc
      481 gcgcgcggcc tgggcatgcg caccgcgtgg atgcagcgct atctcgaggg ccgctaccgt
      541 ggccatctgc gcggcggcgc gcatcccggc tcgaacgccg gcttccgccg gcgcgaagtt
      601 ggtgttcacc cctgcccaag cccccgctac gtgtgtgcca aaatccgctc gctgcaacag
      661 ttgcttacgc tgtgatggat accaacgccg aagccagcgc cgccgaaaac accgacccga
      721 tcgacgccgc agccttggcc gaggcgcccg cgccgcccgt tcgcaagcgc cccaagccgg
      781 gcgagcgccg tgtgcagatc ctgcagacgc tggccagcat gctcgagcag cccggcaccg
      841 accgcatcac gaccgccgcg ctggccgccg agctcgatgt ttcggaggcc gcgctgtacc
      901 gccacttcgc cagcaaggcg cagatgttcg agggcctgat cgaattcatc gagcagaccg
      961 tgttcacgct gatcaaccag atccacgagc gcgaagcggc gtccaacctg caggtgcaga
     1021 agatgctcac ggtgctgctg cagttcgccg agaaaaaccc cggcatggcg cgcgtgatgg
     1081 tcggcgacgc gctggtgttc gagaacgagc gcctgctcgc gcgcatgaac cagtttttcg
     1141 agcgcttcga gtcgcagttg cgccagagcc tgcgtggcgt ggccgaggcg gccggttcgg
     1201 cgacgccgac ggtcgacgcg aacgtgcagg cctcggtgct caccgcgttc gcggtcggcc
     1261 ggctgcagcg cttcgcgcgc tcgggcttca agcgcacccc ggccgagcac ctcgacgccg
     1321 cgctgcagat gctggccgcg taggccgcgc cgctacactg ccgcgtcgcc gcgcctggcg
     1381 gcgcgttttt cgtcccccgc atgccgaccc ctaccgatcc gttgctgcac ctgatcgcgc
     1441 gcgccgaagc gctgctcggg cgcctcgaag ccgtgctgcc gcacccgccg accgccccgg
     1501 actggaacgc ggcgatcgcg ttccgctacc gcaagcgcgg cggctcgggc gtgatcgagc
     1561 cggtgcggca cgtcgcgccg atccggctcg ccgcgctggt ggaggtcgag gcgcaaaagg
     1621 agcggctgct gcgcaacacc cagcagttcg tcgatggcct caaggcgaac aacgtgctgc
     1681 tgacgggcgc gcgcggcacc ggcaagagct cgctgatcaa ggcctgcctg aacgagttcg
     1741 ccgatcgcgg gctgcgcctg atcgaggtcg acaagaccga tctggtcgac ctgcccgaca
     1801 tcgtcgatct cgtcgccgag cgccccgagc gcttcatcgt gttctgcgac gacctcagct
     1861 tcgacgaggg cgagcccggc tacaaggcgc tcaagtcgat cctcgacggc tcggtggcgc
     1921 agtcgtccga caacgtgctg atctacgcga ccagcaaccg ccggcacctg ctgcccgagt
     1981 acatgaagga gaacctcagc taccagcacc tcgagaacgg cgaggtgcac ccgggcgagg
     2041 ttgtcgaaga gaagatctcg ctgtccgagc gcttcgggct gtgggtcagc ttctacccgt
     2101 tcacccaggc cgagtacctg acgatcgtcg cgcagtggct gcgcggctgc ggcgtcgacg
     2161 aggtggcgat tgccgcggcg cgccaggagt cgctggtgtg ggcgctc
//
LOCUS       DASRDG010000413         3578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_517838, whole genome shotgun sequence.
ACCESSION   DASRDG010000413 DASRDG010000000
VERSION     DASRDG010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517838"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFA35_06835"
     CDS             complement(<1..639)
                     /locus_tag="VFA35_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZT55923.1"
                     /translation="MQVKLNTILAAALVMIGGASYAQDMVVKIGHVAPTSGGQAHLGK
                     DNENGAKMAIDELNAKGVTIGGKKAKFELMAEDDAADPKQGTAVANKLVDAKVNGVIG
                     HLNSSTSIPASKIYSDAGIPQITPSSTNPKLTRQGFKTTFRVVADDVHLGGTLGKYAV
                     KELKGKSIAVIDDRSAYGQGVAEEAMKGIKAAGGTVVDQQYTSDKATDFTAIL"
     gene            complement(769..1146)
                     /locus_tag="VFA35_06840"
     CDS             complement(769..1146)
                     /locus_tag="VFA35_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55924.1"
                     /translation="MTSGALPPDRVPTLTEVVAWPQPPAAAAAQLPLPAVEARPAEAD
                     LLSAHSPPLRVAEAQMIEGVLLEVQRQVELMLDYRLREALTPLLARAADGIVREARTE
                     LASTLRDVVARAVAQELARHRKA"
     gene            complement(1143..1589)
                     /locus_tag="VFA35_06845"
     CDS             complement(1143..1589)
                     /locus_tag="VFA35_06845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HZT55925.1"
                     /translation="MTEVSFHFNVADRTDYTCRLVRKATRAGASVVLAGPALALQHFD
                     RALWTFDDLEFIPHVMQSPGQPVAARLRAATQVWLLQDAGAAPQHDVLVNLGTEAPAG
                     FESFAKLIEIVTPDEGDRSAARLRWKHYAARGYAIRKHEVAAGVSS"
     gene            complement(1615..3075)
                     /locus_tag="VFA35_06850"
     CDS             complement(1615..3075)
                     /locus_tag="VFA35_06850"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZT55926.1"
                     /translation="MDFRHQISSPDTLASLSCDALVLVVTGDGVDPALDAPLAAALGD
                     AIHQGDLALKAGKSLYLHRPAGIRAARVAFVAAGGSSLKAFKAAVATGIDLVKGSGAK
                     HVAVGLAGAGTLNDAHAEAVVTAVRESTYLYRHTKPSAPAASKIDKVAVLCAKADAKA
                     VQQGIARGHAIAAGVNLAREWANRPANHCTPTHLAEVAVKLGKEYELKVEVLDRKACE
                     KLGMGSFLAVAQASDEPARFIVARYDGAPKSQAPVVLVGKGITFDTGGISLKPGAEMD
                     EMKYDMGGAASVLGTLRAVAELEPKLNLVCIVPSCENMPGGNAVKPGDVVKSMSGQTI
                     EILNTDAEGRLILCDALTYAERFKPAVVVDVATLTGACVIALGHHHSGLFTADDKVAA
                     QMLEAGAKAIDPCWRMPLDEEYDAGLKSNFADMANIAGRPGGAITAAMFLKRFTAKYP
                     WAHLDIAGTAWKSGAAKGATGRPVPLLTHFVLAQLG"
     gene            3105..>3578
                     /locus_tag="VFA35_06855"
     CDS             3105..>3578
                     /locus_tag="VFA35_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZT55927.1"
                     /translation="MLFDSTLRRELARNFGATLVVILTIVLTMMLIRTLGQAAGGQVA
                     PQDVVLLLGYVALGHLPTMLALSLFIAVVATLSRMYRESEMTIWFASGIGLTRFVAPV
                     LRLSWPVLLVIGLLALFVWPWQNLRGVELKERFERRSDLSRVTAGQFQTSNDGTRV"
BASE COUNT          499 a         1230 c         1229 g          620 t
ORIGIN      
        1 caggatggcg gtgaagtcgg tcgccttgtc gcttgtgtac tgctggtcga cgacggtgcc
       61 gccggcggcc ttgatcccct tcatcgcttc ttcggcgacc ccttggccgt aggcgctgcg
      121 gtcgtcgatc acggcgatgg acttgccctt gagttccttg accgcgtact tgccgagcgt
      181 gccgccgagg tgcacgtcat cggcgacgac gcggaaggtc gtcttgaagc cttggcgcgt
      241 cagtttcggg ttggtcgacg acggcgtgat ctgcgggatg cccgcgtcgc tgtagatctt
      301 cgaggccgga atcgaggtgc tggagttcag gtggccgatg acgccgttga ccttggcgtc
      361 gaccagcttg ttcgcgaccg cggtgccctg cttcgggtcg gccgcatcgt cttccgccat
      421 cagttcgaac ttggctttct tgccgccgat ggtcacgccc ttggcgttga gttcgtcgat
      481 cgccatcttg gcgccgttct cgttgtcctt gccgaggtga gcttggccgc cgctggtcgg
      541 cgccacgtgg ccgatcttca cgaccatgtc ttgggcgtac gaggcgccgc cgatcatcac
      601 cagcgctgcg gcgaggatcg tgttcagttt gacttgcatg caaaacctcc tgaaggattg
      661 ttcaaacaat ggaaacggtt gagatctcaa cgggcgacac cataccccaa atgaaacccg
      721 ggccctatag tggtgaatgc cgggtcgccc gcatccacaa cgcgggggtc aagccttgcg
      781 atgacgtgcc agttcttgtg ccaccgcgcg cgccacgaca tcacgcaagg tggatgccag
      841 ttcggtgcgg gcctcgcgca cgatgccgtc ggcggcacgc gcgagcagcg gggtcagcgc
      901 ctcgcgcagg cggtagtcga gcatcagttc gacctggcgc tgcacctcga gcagcacgcc
      961 ctcgatcatc tgcgcctcgg ccacgcgcag cggcggcgag tgcgcgctca gcaggtcggc
     1021 ctcggcgggg cgtgcctcga cggcgggcag cggcaattgc gccgcagcgg ccgcgggcgg
     1081 ctgcggccac gccacgacct cggtcaacgt cggcacgcgg tcgggcggca gcgccccgct
     1141 ggtcatgacg acacccctgc cgccacctcg tgcttgcgga tcgcgtagcc ccgcgccgcg
     1201 tagtgcttcc agcgcagccg cgcggcactg cggtcgccct cgtcgggcgt gacgatctcg
     1261 atcagcttcg cgaagctctc gaagcccgcc ggcgcctcgg tgccgaggtt gaccagcacg
     1321 tcgtgctgcg gcgccgcgcc ggcgtcctgc agcagccaga cctgggtcgc cgcgcgcagc
     1381 cgcgcggcga ccggctggcc gggcgactgc atcacgtgcg ggatgaactc gaggtcgtcg
     1441 aaggtccaga gcgcccggtc gaagtgctgc agcgcgagcg ccgggccggc gagcacgacg
     1501 ctggcgcccg cgcgcgtcgc cttgcgcacc agacggcacg tgtagtccgt gcggtcagcg
     1561 acattgaaat gaaagctcac ttcggtcatg ccaggctcga cgcgcgcttg ccgctcaacc
     1621 cagctgcgcc agcacgaagt gcgtcagcag cgggacgggc cggccggtcg cgcccttcgc
     1681 ggcgcccgac ttccaagccg tgccggcgat gtcgaggtgc gcccacgggt acttggcggt
     1741 gaagcgcttc aggaacatcg ccgcagtgat cgcgccgccc gggcggccgg cgatattggc
     1801 catgtcggcg aagttgctct tcagcccggc gtcgtactcc tcgtcgagcg gcatgcgcca
     1861 gcacgggtcg atggccttcg cgcccgcctc gagcatctgc gccgcgacct tgtcgtcggc
     1921 ggtgaacagg cccgagtggt ggtggccgag cgcgatcacg caggcgccgg tcagcgtcgc
     1981 cacgtcgacc acgaccgccg gcttgaagcg ctcggcgtag gtcagcgcat cgcacaggat
     2041 caagcggcct tcggcgtcgg tgttcaggat ctcgatcgtc tggcccgaca tgctcttgac
     2101 cacgtcgccc ggcttcaccg cattgccgcc cggcatgttc tcgcacgacg ggacgatgca
     2161 gaccaggttc agcttcggct cgagttcggc cacggcgcgc agcgtgccga gcacgcttgc
     2221 ggcgccgccc atgtcgtact tcatctcgtc catctcggcg ccgggcttca gcgagatgcc
     2281 gccggtgtcg aaggtgatgc ccttgccgac cagcaccacc ggcgcctgcg acttcggcgc
     2341 gccgtcgtat cgcgcgacga tgaagcgcgc cggctcgtcg gaggcttgcg cgaccgccag
     2401 gaacgagccc atgccgagct tctcgcaggc cttgcggtcg agcacctcga ccttcagctc
     2461 gtattccttg ccgagcttca cggccacctc ggccaggtgc gtcggcgtgc agtggttggc
     2521 cggacggttc gcccactcgc gcgccaggtt cacgccggcg gcgatcgcgt ggccgcgcgc
     2581 gatgccctgc tgcaccgcct tcgcgtcggc cttggcgcag agcacggcga ccttgtcgat
     2641 cttggaagcg gccggcgcgc tcggcttggt gtggcgatac aggtaggtgg attcgcgcac
     2701 cgcggtgacg accgcctcgg cgtgcgcatc gttcagcgtg ccagcgccgg ccaggccgac
     2761 ggccacgtgc ttcgcgccgc tgcccttcac gagatcgatg ccggtcgcaa ccgccgcctt
     2821 gaacgccttc aggctcgagc cgccggccgc gacgaacgcg acgcgcgcgg cgcggatgcc
     2881 cgccgggcgg tgcaggtaga ggctcttgcc ggccttcaac gccagatcgc cctgatggat
     2941 cgcatcaccg agcgcggcgg ccagcggcgc atcgagcgcg gggtcgacgc cgtcgccggt
     3001 gacgaccagg acgagcgcgt cgcagctgag gctggcaagg gtatcgggag acgaaatctg
     3061 atgacgaaag tccataatcg acgggtccgt tagacgaacc aatgatgtta ttcgattcca
     3121 ctttgcgccg agagctggcg cgcaacttcg gcgcgaccct ggtggtcatc ctcaccatcg
     3181 tgctgacgat gatgctgatc cgcaccctcg gccaagcggc cggcgggcag gtcgcgccgc
     3241 aggacgtggt gctgctgctc ggctatgtcg cgctcggcca cctgccgacg atgctggcgc
     3301 tgtcgctgtt catcgcggtc gtcgcgacgc tctcgcgcat gtaccgcgag agcgagatga
     3361 cgatctggtt cgccagcggc atcggcctga cgcgcttcgt cgcgccggtg ctgcgcctga
     3421 gctggccggt gctgctggtg atcggcctgc tcgcgctgtt cgtgtggccg tggcagaacc
     3481 tgcgcggcgt cgagctgaaa gagcgcttcg agcgccgctc cgatctgtcg cgcgtcaccg
     3541 ccggccagtt ccagacctcg aacgacggca cccgcgtg
//
LOCUS       DASRDG010000414         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_176220, whole genome shotgun sequence.
ACCESSION   DASRDG010000414 DASRDG010000000
VERSION     DASRDG010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176220"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VFA35_06860"
     CDS             <1..1607
                     /locus_tag="VFA35_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HZT55928.1"
                     /translation="DAVVAVVDATNLRMNLRLVLELKRRGRPMMVALNMADMARSQGL
                     NIDVAKLSAELGCPVVETVAVQHDGHAGLLALMQRESAAARPVPIAAGSAAPSTPAEL
                     QREVRRILAIVEPGITPDAANTKRFHHRLDAVVMHPVWGLAILAAVLFLIFQAVFSWA
                     NVPMDAIKAGMAWAGAWVTAHMADGPLRSLLVDGVIAGLGSVIVFLPQILILFFFILI
                     LEDSGYLPRAAFLLDNLMGKVGLSGRAFIPLLSSFACAIPGIMATRTIANWKDRLATI
                     MIAPLMTCSARLPVYALLIGAFVPERSVGIFNLRGLTLFGLYVAGVVAATLVAWVFKR
                     VWIKSRYQPLMLELPPYRAPGLRNLALGLRERAHIFLARVGGIIFALMVILWFLSSYP
                     APPADATGPAIQYSIAGWLGQALQHVFAPIGFNWQISIALVPGLAAREVAVGALGTVY
                     SMSAAGSEVADALAPVIAKGWSLATAYSLLAWYVFAPQCISTLAVVKRETNSWRYPLL
                     MAAYLFALAYAAAFVTYRVTLALGGG"
     gene            1611..1868
                     /locus_tag="VFA35_06865"
     CDS             1611..1868
                     /locus_tag="VFA35_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55929.1"
                     /translation="MQTLIVALLVIGCGAYAAWTLMPAAARRALASSLLRLPLPAALA
                     AKLRKAATVSSGCGCDGCDRAPATTSPRAPQVVNFHPRSKR"
     gene            complement(1871..>2463)
                     /gene="ispH"
                     /locus_tag="VFA35_06870"
                     /gene_synonym="lytB"
     CDS             complement(1871..>2463)
                     /gene="ispH"
                     /locus_tag="VFA35_06870"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858903.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZT55930.1"
                     /translation="KGHPEVEGTMGQLTEGIWLVEDVADVATVQVVQSAKLAVVTQTT
                     LSVDDAAEILAAVKRRFPQVREPKQQDICYATQNRQDAVKVLAPTVDIVIVVGSPTSS
                     NSNRLRELAQRLGTEAYMVDSPDDLQPQWFEGRRRVGLTAGASAPDILVQQVIARLRA
                     LGAVAVRSIQGVEETVHFPLPKGLGDKAMGLAAPNT"
BASE COUNT          307 a          899 c          843 g          414 t
ORIGIN      
        1 ccgacgcggt cgtcgcggtc gtcgacgcga ccaacctgcg catgaacctg cgcctcgtgc
       61 tcgaactcaa gcgccgcggc cgcccgatga tggtcgcgct gaacatggcc gacatggcgc
      121 gctcgcaggg gctgaacatc gacgtcgcga agctgagcgc cgaactcggc tgcccggtcg
      181 tcgagacggt cgcggtccag cacgacggcc acgccggact gctcgcgctg atgcagcgcg
      241 agtccgccgc cgcccggccg gtgccgatcg cggccggcag cgctgcgccc agcacgccgg
      301 cagagctgca acgcgaggtg cgccgcatcc tcgcgatcgt cgagcccggc atcacccccg
      361 acgcagcgaa cacgaagcgc ttccaccatc gcctcgacgc ggtcgtgatg cacccggtct
      421 ggggcctcgc gatcctggcg gcggtgctgt tcctgatctt ccaggccgtg ttctcgtggg
      481 ccaacgtgcc gatggacgcg atcaaggccg gcatggcctg ggccggcgca tgggtcaccg
      541 cgcacatggc cgacggcccg ctgcgctcgc tgctcgtcga cggcgtgatc gcgggcctgg
      601 gcagcgtgat cgtgttcctg ccgcagatcc tgatcctgtt cttcttcatc ctgatcctcg
      661 aagattcggg ctacctgccg cgcgccgcct tcctgctcga caacctgatg ggcaaggtgg
      721 gcctgtcggg ccgcgccttc atcccgctgc tgtcgagctt cgcgtgcgcg atccccggga
      781 tcatggcgac gcgcacgatc gcgaactgga aggaccggct cgccacgatc atgatcgcgc
      841 cgctgatgac ctgctcggcg cggctgccgg tctacgcgct gctgatcggc gcgttcgtgc
      901 ccgagcgcag cgtcggcatc ttcaacctgc gcgggctcac gctgttcggc ctgtacgtgg
      961 ccggcgtggt cgcggcgacg ctggtggcgt gggtcttcaa gcgcgtctgg atcaagagcc
     1021 gctaccagcc gctgatgctc gaactgccgc cgtaccgcgc acccgggctg cgcaacctcg
     1081 cgctcgggct gcgcgagcgc gcccacatct ttctggcgcg cgtcgggggg atcatcttcg
     1141 cgctgatggt gatcctgtgg ttcctctcga gctacccggc accacccgcc gacgcgaccg
     1201 gcccggcgat ccagtacagc atcgccggct ggctcggcca ggcgctgcag cacgtgttcg
     1261 cgccgatcgg tttcaactgg cagatctcga tcgcgctggt gccggggctg gcggcgcgcg
     1321 aggtcgcggt cggcgcgctc ggcacggtgt actcgatgtc ggccgccggc agcgaggtgg
     1381 ccgacgcgct cgcgccggtg atcgcgaagg gctggtcgct cgcgaccgcg tactcgctgc
     1441 tggcctggta cgtgttcgcg ccgcagtgca tctcgacgct ggcggtcgtc aagcgcgaga
     1501 ccaactcgtg gcgctacccg ctgctgatgg ccgcctatct gttcgcgctc gcctacgcgg
     1561 ccgcgttcgt gacctaccgc gtcaccctgg cgctcggagg gggttgaacc atgcagaccc
     1621 tgatcgttgc cctgctcgtg atcggctgcg gcgcttacgc ggcctggacg ctgatgcccg
     1681 ccgccgcgcg ccgcgcgctc gcttcatcgc tgctcaggct gccgttgccg gccgccctcg
     1741 ccgcaaagct gcgcaaggcc gcgaccgtgt cgtcgggctg cggctgcgac ggctgcgatc
     1801 gcgcgccggc cacgacctca ccgcgggcgc cgcaagtcgt caacttccac ccgcgctcga
     1861 aacgttgata tcaggtgttg ggcgcggcca ggcccatcgc cttgtcgccc aggcccttcg
     1921 gcagcgggaa gtggaccgtc tcctcgacgc cttgaatgct gcgcaccgcc accgcgccga
     1981 gcgcgcgcag ccgcgcgatc acctgctgca ccaggatgtc gggcgccgac gcgccggcgg
     2041 tcaggccgac gcggcgccgg ccctcgaacc attgcggctg caggtcgtcg ggcgagtcga
     2101 ccatgtaggc ctcggtgccc aggcgctgcg cgagctcgcg caggcggttg ctgttcgagc
     2161 tggtcgggct gccgacgacg atcacgatgt cgacggtcgg cgcgagcacc ttgacggcgt
     2221 cctgccggtt ctgggtcgcg tagcagatgt cctgctgctt cggctcgcgc acctgcggaa
     2281 agcgccgctt caccgccgcg aggatctcgg ccgcgtcgtc gaccgacagc gtcgtctgcg
     2341 tcacgaccgc gagcttggct gactgcacga cctgcacggt cgcgacgtcg gccacgtcct
     2401 cgacgagcca gatgccttcg gtcagctgcc ccatcgtgcc ctcgacctcg gggtggccct
     2461 tgt
//
LOCUS       DASRDG010000415         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_988123, whole genome shotgun sequence.
ACCESSION   DASRDG010000415 DASRDG010000000
VERSION     DASRDG010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988123"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="VFA35_06875"
     CDS             <1..1679
                     /locus_tag="VFA35_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HZT55931.1"
                     /translation="LMLLPIETRLDGLSLAELRGQRRAWTIRALPVSDPERLAEALQA
                     VTRTKFVGREQARELGFWSDEAPDDNPPVDDEGRVEVPAWRHALINYPHPLLKQGLVV
                     LDTPGLNAIGAEPELTLSLLPSAHATVFILGADTGVTKSDLSVWRDHLGTHALSRFVV
                     LNKIDALADPLASAAQVDLQIVAQCVETARTLGVGVERVFPLSARQALAARIAGDAAG
                     LQMSRLPALEAALGAQLLPQRRQVLEQIVVDGAQQLETHVARRLGDQRRQLAEQMFEL
                     RGLRGKSGGKLRLMIERIDAETAEFEQCTVRLQALRVVHGRMLKDALVGLSSDRLRDE
                     VAEMQTQMNASVLNLGAKKAFFALCMRLRKRFDEAQKRSNEIRDMLGASFGKLNGEFG
                     FSLSMTKSPGMARFVAELGEIETGYTQYLGLTQALRLSNPKFMEQFRRMLVSKLRVVF
                     ENASAELELWNKATSSQVDSQLRERRRNFRRRRESLERVQTAASDLENRIAELEAHDM
                     RLQGFLRRAKQLFDALRAQAQAGPVAGNELISLQLPQAPADAPARQRAQA"
     gene            1666..>2158
                     /locus_tag="VFA35_06880"
     CDS             1666..>2158
                     /locus_tag="VFA35_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009972864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZT55932.1"
                     /translation="MPRPEPAAFAARLIDWQRSHGRHTLPWQNTRDPYRVWLSEIMLQ
                     QTQVATVLGYYERFLQRFPDVTALAAASLDDVFALWSGLGYYSRARNLHRCAQAVVAE
                     HGGTFPRSAAQLAELPGIGRSTAAAIATFAFGARAAILDGNVKRVLARHRGIGGYPGH
                     AKVE"
BASE COUNT          331 a          776 c          752 g          299 t
ORIGIN      
        1 cgctgatgct gttgccgatc gagacccgcc tcgacggcct gtcgctcgcc gaactgcgcg
       61 gccagcgccg cgcctggacg atccgcgcgc tgccggtcag cgacccagaa cgcctggccg
      121 aagcgctgca ggcggtgacc cgtaccaagt tcgtcggccg cgagcaggcg cgcgaactcg
      181 gcttctggag cgacgaggcg cccgacgaca acccgccggt cgacgacgaa ggtcgcgtcg
      241 aagtgccggc ctggcgccat gcgctgatca actacccgca ccccttgctg aagcagggcc
      301 tggtcgtgct cgacacgccg ggcctgaacg cgatcggcgc cgagcccgag ctgacgctga
      361 gcctgctgcc gagcgcgcac gcgaccgtgt tcatcctcgg cgccgacacc ggcgtgacca
      421 agtccgacct cagcgtctgg cgcgaccacc tcggcacgca cgcgctgtcg cgcttcgtcg
      481 tgttgaacaa gatcgatgcg ctggccgacc cgctcgccag cgccgcgcag gtcgacctgc
      541 agatcgtcgc gcaatgcgtc gagaccgcgc gcacgctggg cgtcggcgtc gagcgcgtct
      601 ttccgctctc ggcgcgccag gcgctcgccg cgcgcatcgc cggcgacgcc gccgggctgc
      661 agatgagccg cctgcccgcg ctcgaagcgg cgctcggcgc gcagctgctg ccgcagcgcc
      721 gccaggtgct cgaacaaatc gtcgtcgacg gcgcgcaaca gctcgagacc cacgtcgcac
      781 ggcgcctggg cgaccagcgc cgccagctcg ccgagcagat gttcgagctg cgcggcctgc
      841 gcggcaagag cggcggcaag ctgcggttga tgatcgagcg catcgacgcc gaaacggcag
      901 agttcgagca gtgcaccgtg cgcctgcagg cgctgcgcgt cgtgcacggc cggatgctga
      961 aggacgcgct ggtcggcctg tcgtccgacc ggctgcgcga cgaagtggcc gagatgcaga
     1021 cgcagatgaa cgcgtcggtg ctgaacctcg gcgcgaagaa ggcgttcttc gcgttgtgca
     1081 tgcggctgcg caagcggttc gacgaggcgc aaaagcgcag caacgagatc cgcgacatgc
     1141 tcggcgcgtc gttcggcaag ctcaacggcg agttcggctt cagcctttcg atgaccaagt
     1201 cgcccgggat ggcgcgcttc gtcgccgagc tcggcgagat cgagaccggc tacacccaat
     1261 acctcgggct gacgcaggcg ctgcggctgt cgaaccccaa gttcatggag cagttccggc
     1321 gcatgctggt gtcgaagctg cgcgtcgtgt tcgagaacgc gagcgccgag ctcgagctct
     1381 ggaacaaggc aacctcgtcg caggtcgatt cgcagctgcg cgagcggcgc cgcaacttca
     1441 ggcgccggcg cgagtcgctc gagcgcgtgc agacggcggc cagcgacttg gagaatcgca
     1501 tcgccgaact cgaagcgcac gacatgcggc tgcagggctt tctgcgccgc gcgaagcagc
     1561 tgttcgacgc gctgcgcgcg caggcccagg ctgggccggt cgccggcaac gagctgatct
     1621 cgctgcaact gccgcaggcc ccggccgacg cgcccgcgcg ccagcgtgcc caggcctgag
     1681 ccggccgcgt tcgcggcgcg gctgatcgac tggcagcgat cccacggccg ccacacgctg
     1741 ccgtggcaga acacacgcga cccgtaccgc gtgtggctct cggagatcat gctgcagcag
     1801 acccaggtcg cgaccgtgct gggctactac gagcgctttc tgcagcgctt tcccgatgtg
     1861 accgcgctcg cggccgcgtc gctcgacgac gtgttcgcgc tctggagcgg cctgggctac
     1921 tacagccgcg cgcgcaacct gcatcgctgc gcgcaggccg tcgtcgccga gcatggcggc
     1981 acgttcccgc gcagcgcggc gcagctcgcg gagctgcccg gcatcggacg ctcgacggcc
     2041 gcggcaatcg cgacgttcgc gttcggcgca cgcgcggcga tcctcgacgg caacgtgaag
     2101 cgcgtcctgg cccgccatcg tggcatcggg ggttaccccg gccacgccaa ggtcgaag
//
LOCUS       DASRDG010000416         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_867087, whole genome shotgun sequence.
ACCESSION   DASRDG010000416 DASRDG010000000
VERSION     DASRDG010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867087"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /gene="bamC"
                     /locus_tag="VFA35_06885"
     CDS             <1..565
                     /gene="bamC"
                     /locus_tag="VFA35_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HZT55933.1"
                     /translation="RGMVEVYKGNQVKETTVWQPRPADPQLEAELLSRLMLRLGATEE
                     AVKTAVAATSAPGAPPRARILTDQPGAALQVDDGFDRAWRRVGVALDRSGFTVEDRDR
                     TQGVYFVRYIDPKFAGKEEPGLFSKLLGFMGSKSSDDAAGGPARYRVTVKGDGDRSTV
                     SVLTGAGAPENGEAGKRITSLLLEDLK"
     gene            602..1381
                     /locus_tag="VFA35_06890"
     CDS             602..1381
                     /locus_tag="VFA35_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZT55934.1"
                     /translation="MRFCSLGSGSAGNATLVEATSGTTTSRVLIDCGFSLRELQARLA
                     RIGLGCDDLDAVFITHEHGDHIGCAATLARRHALPLWMSRGTWRAIGEPALPETLLRF
                     ARDGAAIAVGDLQLLPYTVSHDAQEPLQLRVSDGARQLGVLTDVGQVTPHLLASLQRC
                     DALLLECNHDRAMLANSSYPAALKARIGGRLGHLANDTSAQILAACMHAGLKHLVAAH
                     LSERNNTPALARAALAAAYGGVGDDIVVADALRGFVWLDLR"
     gene            complement(1461..1826)
                     /locus_tag="VFA35_06895"
     CDS             complement(1461..1826)
                     /locus_tag="VFA35_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55935.1"
                     /translation="MTKSLLLASLVAAAALTACGKKEEVPAAAASAAAAPAVAEAASA
                     AASAVSTAASAAATEAMAGASAAMGAASAGTAAAAGAMKDAAGKAADAAKDAAKDAVG
                     KAADAAKEAAGKAADAVKK"
     gene            complement(1977..2465)
                     /locus_tag="VFA35_06900"
     CDS             complement(1977..2465)
                     /locus_tag="VFA35_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55936.1"
                     /translation="MSTEPGSLITSRAEFHAALHQAFETLASAGAREVWLCDEDFADW
                     PLGERATIERLQQWAASSRKLTLVARHFDEVARRHPRWVEWRRNWSHIVSCRSNRELA
                     TGEFPTVLLGLGTVSVRLSDTVHHRGRLSHEKSDEIRCKEQIDAVLQRSDEAFPATTT
                     GL"
     gene            complement(2488..3612)
                     /locus_tag="VFA35_06905"
     CDS             complement(2488..3612)
                     /locus_tag="VFA35_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZT55937.1"
                     /translation="MDTSLPSPLLGGLSPQVFMRRHWQKKPLLVRQAVPGGVDLIDRT
                     QLFNLASHDEVESRLVVRRGAQWSLRPGPMRRAALPPLKQREWTLLVQGLDLHIPAAR
                     ALLDRFRFVPDARLDDLMLSYASDGGGVGPHVDSYDVFLLQAQGRRRWRIGRVGSPEL
                     VPDVPLKILAHFEPEHSLLLEPGDMLYLPPGWAHDGVAEGECMTCSIGFRAGGRDELA
                     REVLQRVLDAADADACGPRYRDPRQGATDAPGRIPPPLQAFAADAVLRLLRDPAALAC
                     ALGEVLSEPKRGVWFDGASDAEAAESVGIALDPRTRMLYDDRHVFINGESFRAGGRDA
                     ALMRRLADRRRLDARERAALSAPARALLGDWLASGWLRAT"
     gene            3637..>3986
                     /locus_tag="VFA35_06910"
     CDS             3637..>3986
                     /locus_tag="VFA35_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZT55938.1"
                     /translation="MLITPPCVVSLTWKLQDTQGTLIDELTEPVEFFYGGDDLLAKVE
                     EALLDQETGFTTTLHLEPEHAFGEYDAALVFFEERSVFPDAIEVGMQFDGPPPGATTP
                     GMPAEAIYTVTEVYP"
BASE COUNT          629 a         1423 c         1367 g          567 t
ORIGIN      
        1 ccgcggcatg gtcgaggtct acaagggcaa tcaggtcaag gagacgacgg tctggcagcc
       61 gcgcccggcc gatccgcagc tcgaggccga gctgctctcg cggctgatgc tgcgcctggg
      121 agcgaccgag gaagctgtca agaccgctgt cgcggcgacg agcgcgcccg gcgcaccgcc
      181 gcgcgcacgc atcctgaccg accagcccgg agcggcgttg caggtcgacg atggcttcga
      241 ccgcgcctgg cgccgcgtcg gcgtcgcgct cgaccgcagc ggcttcacgg tcgaagaccg
      301 cgaccgcacc cagggcgtct atttcgtgcg ctacatcgac ccgaaattcg ccggcaagga
      361 agagccgggg ctgttctcca agctgctcgg cttcatgggc agcaagagca gcgacgacgc
      421 cgccggcggc cccgcgcgct accgcgtgac ggtcaagggc gacggcgacc gcagcaccgt
      481 gagcgtgctg accggcgccg gcgcacccga gaacggcgag gccggcaagc gcatcacctc
      541 gctgctgctc gaagacctga agtaggcccg gcgcggccac acgccaacgg ctggcgcccc
      601 gatgcgcttt tgcagcctgg gcagcggcag cgcgggcaac gccacgctgg tcgaagcgac
      661 gagcggcacg accaccagcc gggtgctgat cgactgcggc ttctcgctgc gcgagctgca
      721 ggcccggctc gcgcgcatcg gcctgggctg cgacgacctc gacgcggtct tcatcaccca
      781 cgaacacggc gatcacatcg gctgcgcggc gacgctggcg cggcgccacg cgctgccgct
      841 gtggatgagc cgcggcacct ggcgcgcgat cggcgaaccc gcgttgcccg agacgctgct
      901 gcgctttgcg cgcgacggcg ccgcgatcgc ggtcggcgac ctgcaactgc tgccctacac
      961 ggtgagccac gatgcccagg agccgctgca gctgcgcgtc tccgacggcg cgcgccagct
     1021 cggcgtgctc accgatgtcg gccaggtcac gccgcacctg ctcgccagcc tgcagcgctg
     1081 cgacgcgctg ctgctcgaat gcaaccacga ccgcgcgatg ctcgcgaact cgagctaccc
     1141 ggccgcgctg aaggcccgca tcggcgggcg gctcgggcac ctcgcgaacg acacgtcggc
     1201 ccagatcctc gcggcgtgca tgcacgcggg gctgaagcat ctcgtcgccg cgcacctgag
     1261 cgagcgcaac aacacgcccg cgctggcgcg cgccgcgctg gccgccgcgt acggcggggt
     1321 cggcgacgac atcgtggtcg ccgacgcgtt gcgggggttc gtctggctcg acttgcgctg
     1381 aggtccgcgc cgggcgcaaa aaaagccgcc cgaaggcggc tctgtctcga agccggccga
     1441 agccggtcgt ctttgccgat ttacttcttg accgcgtcgg cggccttgcc ggcagcttcc
     1501 ttggcggcgt cggcagcctt gcccacggcg tccttggcag cgtccttggc ggcgtctgca
     1561 gccttgcccg cggcgtcctt catcgcgccg gcagcggcag cggtgccggc cgaggcagcg
     1621 cccatcgcag cgctggcacc agccatcgct tcggtcgcgg cagccgaggc ggcggtcgaa
     1681 acagcgctgg cagcagccga agcagcctca gcaacagccg gagcggcggc ggccgaagca
     1741 gccgcggcgg ggacttcttc cttcttgccg caagcggtca aggcagcagc ggcgacgagc
     1801 gaagcgagca aaagcgactt ggtcatgtgt gtgtcctctg gatctgatta gggtcgaaca
     1861 attaccggta attgtgggag cccttacata cgcagagtcc caacgttcac aggcatggac
     1921 agctcgcgcc cttccagtgc ctagcctacg attatagaca gctctagggt gagtccctag
     1981 agcccggtcg tcgttgccgg aaacgcctca tccgaccgtt gcaaaactgc atcaatttgt
     2041 tccttgcaac gaatctcgtc cgacttttcg tgcgataggc gcccgcggtg gtgcacggtg
     2101 tccgacaggc gcacgctgac cgtgcccaga cccaacagca ccgtcgggaa ctcgcccgtt
     2161 gccagctcgc ggttgctgcg gcagctgacg atgtgcgacc agttgcgccg ccactcgacc
     2221 cagcgcggat gacgccgtgc gacctcgtcg aagtggcgcg ccaccagcgt cagcttgcga
     2281 ctcgacgcgg cccattgctg cagccgctcg atcgtcgcgc gctcgccgag cggccagtcg
     2341 gcgaagtctt cgtcgcaaag ccacacctcg cgcgcgccgg cgctggcgag cgtctcgaac
     2401 gcttggtgca gcgcggcgtg aaactcggcg cggctggtga tcagggaccc gggttcggtg
     2461 ctcatcgtcg gtctcctttc gatgcggtca agtcgcgcgc agccagcccg acgccagcca
     2521 gtcgccgagc agcgcgcgcg cgggcgcgct caacgccgcg cgctcgcgcg catcgaggcg
     2581 gcgccggtcg gccaggcgcc gcatcaacgc ggcgtcgcgc ccgccggcgc ggaacgattc
     2641 gccattgatg aacacgtgcc ggtcgtcgta gagcatgcgg gtgcgcgggt cgagtgcgat
     2701 gccgaccgat tctgccgcct cggcgtcgct cgcgccatcg aaccacacgc cacgcttggg
     2761 ttcgctcagc acctcgccga gcgcgcaggc gagcgctgcc gggtcgcgca gcaggcgcag
     2821 caccgcgtcg gcggcgaagg cctgcagcgg cggcgggatg cggcccggcg cgtcggtcgc
     2881 gccctgcctc ggatcgcggt agcgcggccc gcaggcgtcc gcgtcggcgg cgtcgagcac
     2941 gcgctgcagc acctcgcgcg cgagctcgtc gcggccgccg gcgcgaaagc cgatcgagca
     3001 ggtcatgcac tcgccttcgg ccacgccgtc gtgggcccag ccgggcggca ggtagagcat
     3061 gtcgccgggt tcgagcagga gcgaatgctc cggctcgaag tgcgcgagga tcttcagcgg
     3121 cacatcgggc acgagttcgg gcgaaccgac gcgcccgatg cgccagcgcc gccgcccctg
     3181 cgcctgcagc agaaatacgt cgtatgaatc gacatgcggg ccgacgccgc caccatcgct
     3241 cgcatacgac aacatcaggt cgtcgagccg cgcatcgggc acgaagcgga agcgatcgag
     3301 cagcgcacgc gctgccggta tgtgcaggtc caggccttgc acgagcagcg tccattctcg
     3361 ctgcttgagc ggcggcagcg cggcgcgtcg catcggccct ggtcgcagcg accactgcgc
     3421 acctcggcgc acgaccaggc gcgactcgac ctcgtcgtgc gaggcgagat tgaacagctg
     3481 tgtccggtcg atcaggtcga cgccgccggg caccgcctgg cgcaccagca agggcttctt
     3541 ctgccagtgg cggcgcatga aaacctgcgg cgacaggccg cccagcaatg ggctcgggag
     3601 agatgtgtcc atgatcgatt gtgcgatcat cgaacgatgc tcatcacccc accgtgtgtc
     3661 gtcagcctca cctggaagct gcaagacacc caaggcacgc tgatcgacga actgaccgaa
     3721 ccggtcgagt tcttctacgg cggcgacgac ctgctggcga aggtcgaaga agccctgctc
     3781 gaccaggaaa ccggcttcac gaccacgctg cacctggaac ccgagcacgc gttcggcgag
     3841 tacgacgccg cgctcgtgtt cttcgaggag cgttcggtgt ttccggacgc gatcgaggtc
     3901 ggcatgcagt tcgacggccc gccgcccggc gcgaccacgc caggcatgcc ggccgaggcg
     3961 atctacaccg tcaccgaggt ctaccc
//
LOCUS       DASRDG010000417         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_518203, whole genome shotgun sequence.
ACCESSION   DASRDG010000417 DASRDG010000000
VERSION     DASRDG010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518203"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /gene="hisS"
                     /locus_tag="VFA35_06915"
     CDS             complement(<1..903)
                     /gene="hisS"
                     /locus_tag="VFA35_06915"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZT55939.1"
                     /translation="MAERQPKPAALQAVKGMNDILPPESARWEWFEAKVRALMVRYGY
                     ANVRTPIVEPTALFVRGIGAVTDIVEKEMYSFTDAMNGDKLTLRPEATAGIVRAMVEH
                     NALYNGPLRVWSEVSVFRHERPQKGRYRQFHQIDVEALGFAGPDVDAEQILMCRALLI
                     DLGIPTANVRLELNSLGRADERLAHRAALVAHFEAHADLLDADAQRRLHSNPLRILDT
                     KNPAMQAMVAAAPQLMDFLGDASLAHLNAVRAVLDAVGLDYRINPRLVRGLDYYNLTV
                     FEWVTDLLGSQGTVCGGGRYDGLIE"
     gene            complement(1072..2373)
                     /gene="ispG"
                     /locus_tag="VFA35_06920"
                     /gene_synonym="gcpE"
     CDS             complement(1072..2373)
                     /gene="ispG"
                     /locus_tag="VFA35_06920"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZT55940.1"
                     /translation="MSDMNLPSPAFDAIDDFVEPARPPAHRTRQARVAWGSRVVTVGG
                     GAPVRVQAMTNTDTVDAVGTAIQVKELAIAGSEMVRITVNTPEAAAQVPYIREQLDRM
                     GIDVPLIGDFHYNGHRLLTDYPACAEALSKYRINPGNVGKGDKHDRQFAQMVEVAAKY
                     DKAVRIGVNWGSLDQELLTELMDANAQLAEPFEPQQIMYRAILTSAIQSARRAESLGL
                     RGDQIILSCKMSGVQDLISVYRALAKRCDYALHLGLTEAGMGTKGTVASTAALGVLLQ
                     EGIGDTIRVSLTPQPGEARTQEVVVALEILQALGLRQFNPSVTACPGCGRTTSTTFQE
                     LAKQIDDFLRAQMPVWKAKYPGVEAMKVAVMGCIVNGPGESKHADIGISLPGTGEAPA
                     APVFIDGEKALTLRGDNIAAEFHALVERYIDKRFGAVTAGR"
     gene            complement(2381..2710)
                     /locus_tag="VFA35_06925"
     CDS             complement(2381..2710)
                     /locus_tag="VFA35_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HZT55941.1"
                     /translation="MPALTPPVVPAMPAASEPVAAAPDAATLEAAAGVLQFRTTAQSW
                     VEVIDARGQSLIARVLEPGERVGLDGALPMKVKIGNAHGTQVVFKGQAFELAASTRDN
                     VARFELK"
     gene            2709..>2984
                     /locus_tag="VFA35_06930"
     CDS             2709..>2984
                     /locus_tag="VFA35_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55942.1"
                     /translation="MDATDADGTADICGADGAAVAGTAPGVVVDAVVDAVVASAARGR
                     VAAALENRSHSAGIAYTATVAATISPAIHSTGLATSAQSLGTRRPGRS"
BASE COUNT          443 a         1006 c         1052 g          483 t
ORIGIN      
        1 ctcgatcagg ccgtcgtagc gaccgccgcc gcagaccgtg ccctgcgaac cgagcagatc
       61 ggtgacccac tcgaacacgg tcaggttgta gtagtccaga ccgcgcacga gacgcgggtt
      121 gatgcggtag tcgaggccga ccgcgtcgag caccgcgcgc accgcgttca ggtgcgcgag
      181 cgaggcgtcg ccgaggaagt ccatcagctg cggcgccgcc gcgaccatcg cctgcatcgc
      241 agggttcttg gtgtcgagga tgcgcagcgg gttgctgtgc aggcggcgct gcgcgtcggc
      301 gtcgagcagg tcggcgtggg cctcgaagtg cgcgaccagc gcggcgcgat gggcgagccg
      361 ctcgtcggcc cgacccaggc tgttgagctc gagccgcacg tttgccgtcg ggatgcccag
      421 atcgatcagc agcgcgcggc acatcaggat ctgctcggca tcgacgtccg ggccggcaaa
      481 cccgagcgcc tcgacgtcga tctggtgaaa ctgccggtag cggcccttct gcggccgctc
      541 gtggcgaaac accgagacct cggaccagac ccgcagcggc ccgttgtaca acgcgttgtg
      601 ctcgaccatc gcacgcacga tgccggcggt ggcctcggga cgcagcgtga gcttgtcgcc
      661 gttcatcgca tcggtgaagg aatacatctc cttctcgacg atgtcggtca ccgcgccgat
      721 gccgcgcacg aagagggccg tcggctcgac gatcggcgtg cgcacgttgg cgtagccgta
      781 gcgcaccatc agcgcacgca ccttggcctc gaaccactcc cagcgcgccg actcgggcgg
      841 caggatgtcg ttcatgcctt tgacggcttg cagggcagcg ggtttgggtt gtcgttcagc
      901 catgagttcg atcggggggt cttgctgtgg gcgggacaag tcttaccgcc aagagcgcca
      961 aggacgcaaa ggacgcaaag aagagatcaa gagtgcattt ccttggcgcc ctttgcgtcc
     1021 ttggcgtcct tggcggtaag acttcgtgga agttctaccc acgccacaac atcaacgccc
     1081 agccgtcacc gcaccgaagc gcttgtcgat gtagcgctcg accagcgcgt ggaactcggc
     1141 cgcgatgttg tcgccgcgca gggtcagggc cttctcgccg tcgatgaaca ccggcgcggc
     1201 cggcgcctcg ccggtgccgg gcaggctgat gccgatgtcg gcgtgcttgc tctcgcccgg
     1261 accgttgacg atgcagccca tcaccgcaac cttcatcgcc tcgacgccgg ggtacttcgc
     1321 cttccagacc ggcatttgcg cgcgcaggaa gtcgtcgatt tgcttcgcga gctcctggaa
     1381 cgtcgtgctc gtggtgcggc cacagcccgg gcaggcggtg acgctggggt tgaactggcg
     1441 caggccgagc gcttgcagaa tctcgagcgc gaccaccacc tcctgggtgc gcgcctcgcc
     1501 cggctgcggc gtcagcgaga cgcggatggt gtcgccgatg ccttcttgca gcagtacgcc
     1561 caaagcggcg gtcgacgcga ccgtgccctt cgtgcccatg ccggcttcgg tcagaccgag
     1621 gtgcagcgcg tagtcgcagc gcttggccag tgcgcggtag acgctgatca gatcctgcac
     1681 gcccgacatc ttgcacgaca ggatgatctg gtcgccgcgc agccccagcg actcggcgcg
     1741 ccgcgccgac tggatcgccg acgtgaggat cgcgcggtac atgatctgct gcggctcgaa
     1801 cggctcggcg agctgcgcgt tcgcgtccat cagctcggtc agcagttcct gatccaggct
     1861 gccccagttg acgccgatgc gcaccgcctt gtcgtacttg gccgcgacct cgaccatctg
     1921 cgcgaactgg cgatcatgct tgtcgccctt gccgacgttg ccggggttga tgcggtactt
     1981 gctgagcgcc tcggcgcagg cggggtagtc ggtcagcagg cggtggccgt tgtagtggaa
     2041 gtcgccgatc agcggcacat cgatgcccat gcggtcgagc tgctcgcgga tgtagggcac
     2101 ttgcgccgcg gcctcgggcg tgttgacggt gatgcgcacc atctcggagc cggcgatcgc
     2161 gagctccttg acctggatcg cggtgccgac cgcgtcgacc gtgtcggtgt tggtcatcgc
     2221 ctgcacgcgc accggcgcgc cgccgccgac cgtgacgacg cgcgaacccc aggccacccg
     2281 cgcctggcgc gtgcggtgcg cgggcgggcg cgcaggttcg acgaagtcgt cgatcgcgtc
     2341 gaaggcgggc gaaggcaggt tcatgtcgct catgggaggc ctacttgagt tcgaaccggg
     2401 ccacgttgtc gcgcgtcgag gcggcgagct cgaacgcctg gcccttgaag acgacctggg
     2461 tgccgtgcgc gttgccgatc ttgaccttca tcggcagcgc gccgtccagg ccgacgcgct
     2521 cgcccggttc gagcacgcgg gcgatcagcg actggccgcg cgcgtcgatg acctcgaccc
     2581 aggactgcgc ggtggtgcgg aactgcagca cgccggcggc ggcttcgagg gtcgcagcat
     2641 cgggcgcggc ggcgaccggc tcggaggccg ccggcatcgc tggcaccacc ggcggggtca
     2701 aagcgggcat ggacgccacc gacgcagatg gcaccgccga catctgcggt gcagacggcg
     2761 cggccgtcgc cggcacagcg cccggcgtgg tggttgatgc ggtggtcgat gcggtcgtgg
     2821 catcggccgc gcgcggccgg gtcgcggcgg ccttggaaaa tcgcagccat tcggccggca
     2881 tcgcgtacac cgcgaccgtc gccgcgacga tcagcccggc aatccacagc accgggctgg
     2941 caacgtcggc ccagtccctc ggcacgaggc gcccggggcg gtcc
//
LOCUS       DASRDG010000418         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1668969, whole genome shotgun sequence.
ACCESSION   DASRDG010000418 DASRDG010000000
VERSION     DASRDG010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668969"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFA35_06935"
     CDS             <1..667
                     /locus_tag="VFA35_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HZT55943.1"
                     /translation="ELNSFAAWRPGDLAGGLAPPTNVHRAADEPPKVDPAVERAAELR
                     AARQAGYQDGYRDGLVALDAFKQSFAAQTSAQIGALLHSLGAELGGLQQEMARTLAVS
                     ATHLARQIVRGELASNPALVANVAQEAIDTLLLSAKHITLRVHPDDQPFVAQGAAEVL
                     AARGVRLLADAAMTRGGVLVESDIGVVDASLEARWRRAAASLGCDEGWTGDAEVESAG
                     SDA"
     gene            822..2246
                     /gene="fliI"
                     /locus_tag="VFA35_06940"
     CDS             822..2246
                     /gene="fliI"
                     /locus_tag="VFA35_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HZT55944.1"
                     /translation="MTPQNSSNPDKWQRYLADMRSFSADKVRLETQGLLVRVTGLVLE
                     AAGIRVPVGSVCEVRMEGQPPVIAEVVGFSGDRAYLMPTGDVAGLASGARVVPRPSPV
                     VPMQLGAARHPWRRNEDRTLHLPVGDGLLGRVVDSHGHPIDRKGPIEHLHKEPLARRP
                     INAMDRDPVRTPLDTGVRAINAMLTIGRGQRIGLFAGTGVGKSVLLGMMARYTAADVI
                     VVGLIGERGREVKEFIEDILGEEGMRRAVVVAAPADAPPLVRMQGANYATVIAEHFRD
                     RGQNVLLLMDSLTRYAMAQREIALAIGEPPATKGYPPSCFAKLPQLVERSGNGKAAGQ
                     EGGGGSITAFYTVLSEGDDQQDPIADAARGILDGHIVLSRELAEAGHYPAIDIEKSVS
                     RVMTSVASREHVEAARRFRHLHARYNKARDLIQLGAYAPGHDAELDTAVRLHEPMVAM
                     LQQNMHESAAMAGSVQQLRSAIGG"
     gene            2270..2737
                     /gene="fliJ"
                     /locus_tag="VFA35_06945"
     CDS             2270..2737
                     /gene="fliJ"
                     /locus_tag="VFA35_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HZT55945.1"
                     /translation="MTNTETLTILLGQHERQRDAAIAEHRRAQDATTAAQGRAEQLRG
                     YRGEYEQRWRAQFGRAGKIELVHCYQSFMERLTLAIAQQSHAAEQAAQQAGRAGVALR
                     DAELRCASVRKLIERRVAEQRLAADRREQKQTDEAATRAAWNRLAATRPAPLM"
     gene            2754..>3169
                     /locus_tag="VFA35_06950"
     CDS             2754..>3169
                     /locus_tag="VFA35_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55946.1"
                     /translation="MLSLNLSSLRGSAGDAATTPATCANGGNPPAGASDPAGFAALLR
                     QTQVAPAPAPVAPVDAHASHEPVPEHDAPDEAPPPRESSDAASRHRALKGKLRPTDGT
                     AVPMRDAHPAPAAHSAQDKADAATRADAAAADTTEKR"
BASE COUNT          497 a         1166 c         1135 g          371 t
ORIGIN      
        1 ggagctgaat tcgttcgcgg cctggcggcc cggcgatctg gcaggcggtc tcgcgccgcc
       61 gacgaacgtg catcgcgccg ccgacgaacc gcccaaggtc gacccggccg tggagcgcgc
      121 cgccgaactg cgcgccgcgc gccaggccgg ctaccaggac ggctaccgcg acggcctggt
      181 cgcgctcgac gccttcaagc agagcttcgc ggcgcagacc tccgcgcaga tcggtgcgct
      241 gctgcactcg ctcggtgccg agctcggcgg cctgcagcag gagatggcgc gcacgctggc
      301 ggtctcggcc acgcacctgg cgcggcagat cgtgcgcggc gagctcgcgt cgaacccggc
      361 gctggtggcg aacgtcgcgc aggaggcgat cgacacgctg ctgctgagcg cgaagcacat
      421 cacgctgcgc gtgcaccccg acgaccaacc cttcgtcgcg cagggcgcgg ccgaggtgct
      481 cgccgcacgt ggcgtgcggc tgctcgccga cgcggcgatg acgcgcggcg gcgtgctggt
      541 cgagtccgac atcggcgtcg tcgacgccag cctggaggcg cgctggcgcc gcgccgcggc
      601 gtcgttgggc tgcgacgagg gctggactgg agatgccgag gtcgaatcgg cggggtccga
      661 cgcatgaatg ggggttcagt gtttatcgag ggcccttttt tgaccgcaaa gaacgcaaag
      721 gacgcaaagg acgcaaagga cgcaaagaaa agaattgctc ttcttggcgt ccttggcgct
      781 cttggcgtcc ttggcggtcg gtcgtttgga tggggcaccc gatgactccg cagaacagca
      841 gcaaccccga caaatggcaa cgctacctgg ccgacatgcg cagcttctcg gccgacaagg
      901 tgcgcctgga gacgcaaggc ctgctcgtgc gtgtgaccgg cctcgtgctc gaggccgccg
      961 gcatccgcgt gccggtcggc tcggtctgcg aggtcaggat ggagggccag ccgccggtga
     1021 tcgccgaggt cgtcggcttc tcgggcgacc gcgcctacct gatgcccacg ggcgatgtgg
     1081 ccggcctcgc gagcggcgcg cgcgtcgtgc cgcggccttc gccggtcgtg ccgatgcagc
     1141 tcggcgcggc gcgccacccg tggcgccgca atgaagaccg cacgctgcac ctgccggtgg
     1201 gtgacggcct gctcggccgc gtcgtcgact cgcacggcca cccgatcgac cgcaaaggcc
     1261 cgatcgagca cctgcacaag gagccgctgg cgcgccgccc gatcaacgcg atggaccgcg
     1321 acccggtgcg caccccgctc gacaccggcg tgcgcgcgat caacgcgatg ctgacgatcg
     1381 ggcgcggcca gcgcatcggc ttgttcgccg gcaccggtgt gggcaagtcg gtgctgctcg
     1441 ggatgatggc ccgctacacc gcggccgacg tgatcgtcgt cggcctgatc ggcgaacgcg
     1501 gccgcgaggt gaaggagttc atcgaggaca tcctcggcga ggaagggatg cgccgcgcgg
     1561 tcgtcgtcgc cgcgccggcc gatgcgccgc cgctggtgcg catgcagggc gcgaactacg
     1621 cgaccgtgat cgccgaacac tttcgcgatc gcggccagaa cgtgctgctg ctgatggact
     1681 cgctgacccg ctatgcgatg gcgcaacgcg agatcgcgct cgcgatcggc gagccgcccg
     1741 cgaccaaggg ctacccgccg agctgcttcg ccaagctgcc gcagctggtc gagcgcagcg
     1801 gcaacggcaa ggccgcgggc caggaaggcg gcggcgggtc gatcaccgcg ttctacaccg
     1861 tgctcagcga gggcgacgac cagcaggacc cgatcgccga tgcggcccgg ggcatcctcg
     1921 acggccacat cgtgctgtcg cgcgagctcg ccgaggccgg ccactacccg gcgatcgaca
     1981 tcgagaagtc ggtctcgcgc gtgatgacga gcgtcgcgag ccgcgagcac gtcgaggcgg
     2041 cgcggcgctt tcgccacctg cacgcgcgct acaacaaggc gcgcgacctg atccagctcg
     2101 gcgcctatgc gccgggccac gacgccgaac tcgacaccgc cgtgcgcctg cacgagccga
     2161 tggtcgcgat gctgcagcaa aacatgcacg agagcgcggc gatggccggc agcgtgcagc
     2221 agctgcgcag cgcgatcggc ggctgatcgc cacgcagaag aacccgaaca tgaccaacac
     2281 cgagaccctg accatcctgc tgggccagca cgagcgccag cgcgacgccg cgatcgccga
     2341 gcaccggcgc gcccaagacg ccaccacggc cgcgcagggc cgggccgagc agctgcgcgg
     2401 ctaccggggc gagtacgagc agcgctggcg cgcgcagttc ggccgcgcgg ggaagatcga
     2461 actcgtccac tgctaccaga gcttcatgga gcggctgacg ctcgcgatcg cgcagcaatc
     2521 acacgcggcc gagcaggcgg cgcagcaggc tgggcgcgcc ggcgtcgcgt tgcgcgacgc
     2581 cgaactgcgc tgcgcctcgg tgcgcaagct gatcgagcgg cgcgtcgccg aacaacgcct
     2641 cgccgccgac cgccgcgagc agaagcagac cgacgaggcc gccacgcgcg cggcctggaa
     2701 ccgcctggcc gccacgcgcc cggcgccgct gatgtgagcc cggagcgacc acgatgctga
     2761 gcctcaacct ctcgtcgttg cgcggctcgg ccggcgacgc ggccaccaca ccggccacct
     2821 gcgcgaacgg cggcaacccg ccggccggcg cgagcgaccc ggccggcttc gcggcgctgc
     2881 tgcgccagac gcaggtcgca cccgcgcccg cacccgtggc gccggtcgac gcgcacgcga
     2941 gccacgaacc ggtgcccgag cacgatgcgc ccgacgaggc cccgccgccg cgggagtcct
     3001 ccgacgcggc cagccgccat cgcgcgctca aaggcaagct gcgcccgacc gacggcacag
     3061 ccgttccgat gcgcgacgcg caccccgcgc ccgccgccca cagcgcgcaa gacaaggccg
     3121 atgccgcgac gcgcgccgac gccgccgccg ccgacaccac cgagaagcg
//
LOCUS       DASRDG010000419         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_867511, whole genome shotgun sequence.
ACCESSION   DASRDG010000419 DASRDG010000000
VERSION     DASRDG010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867511"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFA35_06955"
     CDS             complement(<1..579)
                     /locus_tag="VFA35_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZT55947.1"
                     /translation="MNKIFPSAAAALQGVVKDGQLLAVGGFGLCGIPEALIKALRDSG
                     VKNLTVISNNAGVDGFGLGQLLETRQIKRMISSYVGENKEFERQYLAGELELEFTPQG
                     TLAEKLRAGGAGIPGFYTRTAAGTKLAEGKETKTFDGKEYVLEEGIKADLAMVKAWKG
                     DKSGNLVYRKTSRNFNPMIATCGKVTVAEVEEL"
     gene            717..1283
                     /locus_tag="VFA35_06960"
     CDS             717..1283
                     /locus_tag="VFA35_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZT55948.1"
                     /translation="MTPTATLDYRTAFDLAPIGLVLSRNRLMVDCNRELLEIFGTTRD
                     ALVGRSFELLYPSQAEFEHTGARIVASLNARGGYADERVMKRLGAAQRDLLFWCRVSG
                     RALDPSQPHAAGIWSFEDLSARRRVGVELTPREREIAALLIEGLTSKLIGRRLAISPR
                     TVDVYRARLMRKHGAGTTPELVQRLLRA"
     gene            complement(1287..1706)
                     /locus_tag="VFA35_06965"
     CDS             complement(1287..1706)
                     /locus_tag="VFA35_06965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZT55949.1"
                     /translation="MRFHLPDAKKLVHEMVIAMRWGDMDAMGHVNNTVYFRYMESVRL
                     EWLFKVGAAKDAHGNGPVIVNAFCNFIQQLQYPGDLLAKHYVAKPGRTSFESYITLER
                     TDVPGVICASGGAKTVWVNYAQKKSVPLPTALRKLVA"
     gene            complement(1721..2389)
                     /locus_tag="VFA35_06970"
     CDS             complement(1721..2389)
                     /locus_tag="VFA35_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HZT55950.1"
                     /translation="MQLRPLSLLEARVLGVLVEKAHTVPDSYPLSLNSLTLGCNQKTA
                     REPVLNALESEVQDAVIALKALNLVFEASGSRVTRYEHNAGRALALPSQSIALLATLV
                     LRGPQTVSELRTACERLHRFADLSSVEGFLDELAARLAEKGGALVVRLPRAPGAREPR
                     WAHLLSGAVDVASMPVASDSPDFVAAGEITALKAQHAALQAELNELRGLVERLYAELG
                     VART"
     gene            complement(2392..>2976)
                     /locus_tag="VFA35_06975"
     CDS             complement(2392..>2976)
                     /locus_tag="VFA35_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZT55951.1"
                     /translation="VTDHDSIKAAVAHAETEMGVIDILVNNSGVSTTQKLTDVTPEDF
                     DYVFDVNARGAFFVAQEVGKRMIARSQGAAPGTFTGGRIVNIASMAGLKPLGRIGVYA
                     MSKAAVVHMTRVMALEWGRYGINVNAICPGYIDTEINHHQWGTERGKKLIEMLPRKRV
                     GQPQDLDAVLMMLVANESHFVNGAIIQADDGFGM"
BASE COUNT          442 a         1040 c          974 g          520 t
ORIGIN      
        1 cagctcctcc acttcggcga cggtgacctt gccgcaggtg gcgatcatgg ggttgaaatt
       61 ccgcgaggtc ttgcgataca cgaggttacc ggacttgtcg cccttccagg ccttgaccat
      121 cgccaggtcg gccttgatgc cctcctccag cacgtactcc ttgccgtcga aggtcttggt
      181 ctccttgcct tcggcgagct tggtccccgc cgcggtgcgc gtatagaagc ccgggatacc
      241 ggcgccgccc gcgcgcagct tttcggcgag cgtgccctgc ggcgtgaact cgagctccag
      301 ctcgccggcc aggtactggc gctcgaactc cttgttctcg ccgacgtagc tcgaaatcat
      361 gcgtttgatc tggcgcgtct cgagcaactg gccgaggccg aagccgtcga cgccggcgtt
      421 gttcgagatc acggtcaggt tcttcacgcc cgaatcgcgc agcgctttga tcagtgcctc
      481 ggggatgccg cacaggccga agccgccgac ggccagcagc tggccgtcct tcacgacgcc
      541 ctggagcgcc gcagcggcgc tggggaagat cttgttcatc gggggcactc tcctcggaaa
      601 agacgcacgc gggcacgcat gggttgcagc gcgggagcgt actacgtatg gcattacgta
      661 gtcgttacgt aaaatcgcgt aaggatactt ccgcgtccac cctcgcctgc ccaccgatga
      721 cgccgacggc cacgctcgac taccgcaccg cgttcgacct cgcgccgatc ggcctggtgc
      781 tctcgcgcaa ccgcctgatg gtcgactgca accgcgagtt gctggagatc ttcggcacca
      841 cccgcgacgc gctggtcggc cgtagcttcg agctgctcta cccgtcgcag gccgagttcg
      901 agcacaccgg cgcgcgcatc gtcgcgagcc tgaacgcgcg cggcggctat gccgacgagc
      961 gcgtgatgaa gcgcctcggc gccgcgcagc gcgacctgct gttctggtgc cgcgtgtcgg
     1021 gccgcgcgct cgacccctcg cagccgcacg cggccggcat ctggtcgttc gaggacttgt
     1081 cggcgcggcg gcgtgtcggc gtcgagctca cgccgcgcga gcgcgagatc gccgcgctgc
     1141 tgatcgaggg gctgacgagc aagctgatcg gcaggcggct cgcgatcagc ccgcgcaccg
     1201 tcgatgtgta ccgcgcccgc ctgatgcgca agcacggcgc cggcacgacg cccgagctgg
     1261 tgcagcggct gctgcgcgcc tgagccttac gccacgagct tgcgcagcgc ggtcggcagc
     1321 ggcaccgact tcttctgtgc gtagttcacc cacacggtct tcgcgccgcc cgaggcgcag
     1381 atcacgccgg gcacgtcggt gcgctccagc gtgatgtagc tttcgaagct ggtgcggccc
     1441 ggcttggcca catagtgctt cgcgagcagg tcgccggggt actgcagctg ctggatgaaa
     1501 ttgcagaacg cgttgacgat caccgggccg ttgccgtgcg cgtctttggc ggcgccgacc
     1561 ttgaacagcc attcgagccg caccgactcc atgtagcgaa agtaaaccgt gttgttcacg
     1621 tggcccatcg cgtccatgtc gccccagcgc atggcgatca ccatctcgtg cacgagtttc
     1681 ttggcgtcgg gcaggtggaa tcgcatgcag tgttctctcc tcaggttcgg gccacgccga
     1741 gctcggcata cagccgctcg accaggccgc gcagttcgtt cagttcggcc tgcagcgcag
     1801 cgtgctgcgc cttcagcgcg gtgatctcgc cggcggcgac gaagtctggc gaatcgctcg
     1861 cgaccggcat cgaggccacg tcgaccgcgc cgctcagcag atgcgcccag cgcggctcgc
     1921 gcgcacccgg cgcgcgcggc agcctgacga cgagcgcgcc gcccttctct gccaggcgcg
     1981 ccgcaagttc gtccagaaag ccttcgaccg acgacaggtc ggcgaagcgg tgcagccgtt
     2041 cgcaggccgt gcgcagctcg gacaccgtct gcggcccgcg cagcaccagc gtcgcgagca
     2101 gcgcgatcga ctgcgacggc agcgcgagcg cgcgaccggc gttgtgctcg tagcgcgtca
     2161 cgcgcgagcc gctcgcctcg aagacgaggt tcagcgcctt cagcgcgatc accgcgtctt
     2221 gcacctcgga ttcgagcgcg ttcagcaccg gctcgcgcgc ggtcttctgg ttgcagccga
     2281 gggtcagcga attcagcgac aacgggtagc tgtcgggcac ggtgtgggcc ttctcgacca
     2341 gcaccccgag cacgcgcgct tcgagcagcg acaacggccg cagctgcatg ctcacatgcc
     2401 gaagccgtca tcggcctgaa tgatcgcgcc gttgacgaag tggctctcgt tcgcgaccag
     2461 catcatcagc accgcgtcca gatcctgcgg ctgcccgacg cgcttgcgcg gcagcatctc
     2521 gatcagcttc ttgccgcgct cggtgcccca ctggtggtgg ttgatctcgg tatcgatgta
     2581 gcccgggcag atcgcgttga cgttgatgcc gtagcggccc cattcgagcg ccatcacgcg
     2641 cgtcatgtgc accaccgcgg ccttgctcat cgcatacacg ccgatgcgcc cgagcggctt
     2701 cagccccgcc atcgaggcga tgttgacgat gcgcccgccg gtgaacgtgc ccggcgccgc
     2761 gccctgcgag cgcgcgatca tgcgcttgcc gacctcctgc gcgacgaaga acgcgccgcg
     2821 ggcgttgacg tcgaagacat agtcgaagtc ctccggcgtc acgtccgtga gcttctgcgt
     2881 ggtgctgacg ccggagttgt tcacgaggat gtcgatcacg cccatctcgg tctcggcatg
     2941 cgcgaccgcg gccttgatgc tgtcgtggtc ggtcac
//
LOCUS       DASRDG010000420         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1324282, whole genome shotgun sequence.
ACCESSION   DASRDG010000420 DASRDG010000000
VERSION     DASRDG010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324282"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..452)
                     /locus_tag="VFA35_06980"
     CDS             complement(114..452)
                     /locus_tag="VFA35_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HZT55952.1"
                     /translation="MATAKKAPAKKAAAKKAPAKKAAAKKAPAKKVAAKKAPAKKAAA
                     KKAPAKKAAAKKAPARKAAAKKAPAKKAAAKKAAKKPAAKKSAAAPAAPAKTSLSPQA
                     AWPFPTGSKP"
     gene            complement(712..1920)
                     /locus_tag="VFA35_06985"
     CDS             complement(712..1920)
                     /locus_tag="VFA35_06985"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HZT55953.1"
                     /translation="MLVWEEEVKPSTVHAAALAQTQPSLAQVAPLSPALASMSVSAAN
                     AAPAFEAQMPKVEAAVVATTRRVKAADKRIINGQTDVNQLVPFKYKWAWEKYLATCAN
                     HWMPQEVNMSRDIATWKDPNGLTEDERRIVKRNLGFFVTADSLAANNIVLGTYRHITA
                     PECRQFLLRQAFEEAIHTHAYQYIVESLGLDESEIFNAYNEVQSIRDKDEFLIPFIAA
                     IMDPNFHTGTVENDRTLLKSLIVFACLMEGLFFYVGFTQILALGRQNKMTGAAEQYQY
                     ILRDESMHCNFGIDLINQIKLENPQLWTAEFKAEIKALFQKAVELEYRYAEDTMPRGV
                     LGMNASMFKGYLRYIANRRATQIGLEPLFPNEENPFPWMSEMIDLKKERNFFETRVIE
                     YQSGGALSWD"
     gene            complement(2093..>3786)
                     /locus_tag="VFA35_06990"
     CDS             complement(2093..>3786)
                     /locus_tag="VFA35_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HZT55954.1"
                     /translation="IKGTNGRSQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYLETWHL
                     DLEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVMDGGDWTLFSPSTCPDLHDKFG
                     KDFETAYAAYEAKAASGEIKLFKKTPARDLWRKMLSMLFETGHPWITFKDACNVRSPQ
                     QHVGVVHSSNLCTEITLNTSETEIAVCNLGSVNLVHHIAVDANGVKGIDHAKLKKTVS
                     TAMRMLDNVIDINYYAVKKARDSNLKHRPVGLGIMGFQDALYELGVPYASDAAVDFAD
                     RSMEAVCYQAYWASTELAAERGRYSSYRGSLWDRGILPIDSLDLLAEQRGGYVEVDRS
                     ATLDWDALRGRIREHGMRNSNCTAIAPTATISNIVGVSASIEPSFGNLSVKSNLSGEF
                     TIVNEYLVRDLKKLGLWDDVMVMDLKHFDGSLRRIDRVPADLKALYATAFEIETQWLV
                     EAAARRQKWIDQAQSLNIYMAGASGKKLDETYKLAWLRGLKTTYYLRTVGATHAEKST
                     VKAGHMNAVSSEGGVSKSAAQSAATVDAAMSLGHVDPATDMKFCAIDNPDCEACQ"
BASE COUNT          579 a         1219 c         1181 g          807 t
ORIGIN      
        1 gacgccgcgc gcttgtcggc atcctgagga cacgcggcaa caccgtcacg cgacgccgcc
       61 acaaacaagc agcccggccg aagccgggct gtgtgtcagt cacgcaagac cgatcagggc
      121 ttgctgccgg tcgggaacgg ccatgccgcc tgcgggctca gtgaagtctt cgccggcgca
      181 gcgggtgcgg ccgcgctctt cttcgcggcg ggcttcttcg ctgctttctt cgcagcggcc
      241 ttcttcgccg gcgccttctt cgcggcggcc ttcctggcag gcgccttctt cgcagcggcc
      301 ttcttcgccg gcgccttctt cgcggcggcc ttcttggcag gcgccttctt cgcggcgacc
      361 ttcttcgccg gcgccttctt cgcggcggcc ttcttggccg gggccttctt tgcagcggcc
      421 tttttcgccg gcgctttctt tgcagttgcc atttcgtttc tccttgatca agttgcaaaa
      481 agaactgccc cactccggcg cgccgggatg cgacagctat tcagcgtccg aagtctgccc
      541 aggaggggcg ccccggtacg ttgaatgggg tcgcgattcg cgtgcgccgc ttctttgtcg
      601 gcgcctcacg catcgcaggt cttgatcggt caatgccctt gcccatgccg gcacaagaga
      661 ctcacgcccc ggtgtttgca agcaccgagg ctcttccttt cattcgcaac gtcagtccca
      721 ggacagcgcg ccgccactct ggtactcgat cacacgggtc tcgaagaagt tgcgctcctt
      781 cttcaagtcg atcatctcgc tcatccacgg gaacgggttt tcctcgttcg ggaacagcgg
      841 ctccagaccg atctgcgtcg cacggcggtt cgcgatgtag cgcaggtagc ccttgaacat
      901 cgacgcgttc atgccgagca cgccgcgcgg catggtgtct tcggcgtagc ggtattcgag
      961 ttcgacggcc ttctggaaca gcgccttgat ctcggccttg aattcggccg tccacagctg
     1021 cgggttctcg agcttgatct ggttgatcag gtcgatgccg aaattgcagt gcatcgattc
     1081 atcgcgcagg atgtactggt actgctcggc cgcgccggtc atcttgttct gccgacccag
     1141 cgccaggatc tgcgtgaagc cgacgtagaa gaacaggcct tccatcaggc aggcgaacac
     1201 gatcagcgac ttcagcagcg tgcggtcgtt ctcgaccgtg ccggtgtgga agttcgggtc
     1261 catgatcgcc gcaatgaacg gaatcaggaa ctcgtccttg tcgcggatcg actggacttc
     1321 gttgtaggcg ttgaagatct cgctctcgtc cagacccagc gactcgacga tgtactgata
     1381 ggcgtgggtg tggatcgcct cttcgaacgc ctggcgcagc agaaactgcc ggcactcggg
     1441 cgccgtgatg tggcggtagg tgccgagcac gatgttgttc gccgccagcg aatcggcggt
     1501 cacgaagaag ccgaggttgc gcttgacgat gcggcgctcg tcttcggtca ggccgttcgg
     1561 gtctttccag gtcgcgatgt cgcgcgacat gttgacctct tgcggcatcc agtggttcgc
     1621 gcaggtcgcg aggtacttct cccacgccca cttgtacttg aacggcacca gctgattcac
     1681 atcggtctgg ccgttgatga tgcgtttgtc ggcggccttg acgcgccgcg tggttgcgac
     1741 caccgccgct tcgaccttcg gcatctgcgc ttcaaacgca ggggccgcgt tggccgcgct
     1801 cacgctcatc gatgcaaggg ctggtgacag aggggccact tgcgcgagag acggctgtgt
     1861 ctgcgcaagc gctgcggcgt gaactgtcga gggcttaact tcttcttccc aaaccagcat
     1921 ggtgaaattt tcctgtgcgt tccaattatg agagtgttgc gcaataaccc aaagcacttc
     1981 ttgacatctg cgcgcattgc ttgttgcggc atcgcatcga cgcttcgtga tcgatcatgc
     2041 gtgtgcgaca ccgcagcgct gcatcaactc tctcgcgtca tctgtctcgt gctcactggc
     2101 acgcttcgca gtccgggttg tcgatcgcgc agaacttcat gtcggttgca ggatcgacat
     2161 gcccgaggct catcgccgca tcgacggtcg ctgcgctctg cgccgccgac ttcgacacgc
     2221 caccttcgct cgacaccgca ttcatgtgac cggctttcac cgtcgacttc tccgcatgcg
     2281 tcgcgccgac ggtgcgcagg tagtaggtcg tcttcagacc acgcagccac gcgagcttgt
     2341 aggtctcgtc gagcttcttg cccgacgcgc cggccatgta gatgttgagc gactgcgcct
     2401 gatcgatcca cttctggcgc cgcgccgcgg cttcgaccag ccactgcgtc tcgatctcga
     2461 acgcggtcgc gtacaaggct ttcaggtctg cgggcacgcg gtcgatgcgg cgcagcgaac
     2521 cgtcgaagtg cttgaggtcc atcaccatca cgtcgtccca caggccgagc ttcttcaggt
     2581 cgcgcaccag gtactcgttg acgatcgtga actcgcccga caggttcgac ttgaccgaca
     2641 ggttgccgaa cgacggctcg atcgacgcgc tgacaccgac gatgttcgag atcgtcgcgg
     2701 tcggtgcgat cgcggtgcag ttcgaattgc gcatgccgtg ctcgcggatg cggccgcgca
     2761 gcgcgtccca gtcgagcgtg gcgctgcggt cgacctcgac gtagccaccg cgctgctcgg
     2821 cgagcagatc gagcgagtcg atcggcagga tgccgcggtc ccacagcgag ccgcggtagc
     2881 tcgaatagcg gccgcgctcg gcggcgagtt cggtcgaggc ccaataggcc tggtagcaga
     2941 ccgcttccat cgagcggtcg gcgaagtcga ccgcagcgtc cgacgcgtag ggcacgccga
     3001 gttcgtacag cgcatcctgg aaacccatga tgcccagacc caccggccga tgcttcaggt
     3061 tcgagtcgcg cgccttcttg acggcgtagt agttgatgtc gatcacgttg tcgagcatgc
     3121 gcatcgcggt cgacacggtc ttcttcagct tcgcgtggtc gatgcccttc acgccgttcg
     3181 cgtcgacggc gatgtggtgc accaggttca ccgaaccgag gttgcacacc gcgatctcgg
     3241 tctcgctggt gttcagcgtg atctcggtgc acaggttgct cgagtgcacg acgccgacat
     3301 gctgctgcgg cgagcgcacg ttgcaggcat ccttgaacgt gatccagggg tggccggtct
     3361 cgaacagcat cgacagcatc ttgcgccaca ggtcgcgcgc cggggtcttc ttgaacagct
     3421 tgatctcgcc gctcgcggcc ttggcctcgt aggcggcgta ggccgtctcg aagtccttgc
     3481 cgaacttgtc gtgcaggtcg gggcaggtgc tgggcgagaa cagggtccag tcgcccccgt
     3541 ccatcacgcg cttcatgaac aggtcgggaa tccagttcgc ggtgttcatg tcgtgcgtgc
     3601 ggcgccggtc gtcgccggtg ttcttgcgca gctcgaggaa ctcttcgagg tcgaggtgcc
     3661 aggtctccag gtaggcgcag accgcgccct tgcgcttgcc gccctggttg accgcgaccg
     3721 cggtgtcgtt gacgaccttc aggaacggca ccacgccctg gctcctgccg ttcgtgccct
     3781 tgatgt
//
LOCUS       DASRDG010000421         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_771714, whole genome shotgun sequence.
ACCESSION   DASRDG010000421 DASRDG010000000
VERSION     DASRDG010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771714"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFA35_06995"
     CDS             <1..207
                     /locus_tag="VFA35_06995"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HZT55955.1"
                     /translation="GKGSGLGLRIATDLVLAGVLLFCGSLYALLGGAPRALGALTPFG
                     GVALIAGWGVAAIALLRARDPSAP"
     gene            204..>2027
                     /gene="hrpB"
                     /locus_tag="VFA35_07000"
     CDS             204..>2027
                     /gene="hrpB"
                     /locus_tag="VFA35_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HZT55956.1"
                     /translation="MSATQPAAGAALIEPLRAAFAAHRDVVLRAPPGAGKSTLVPLAL
                     LAEPWLLGRKILLLEPRRVAARAVARRMAYLRGEQVGDTVGYRMRLDTRVSGRTRIEV
                     ITEGVLTRMLQSDPELAGVGCVIFDEFHERSLQADLALALCLDARKTLDGDFRILAMS
                     ATLDGARVAALLGGAPVVDVPGREFEVGVIYCGRGAPLLPGGTEPPERALGRTVRRAL
                     EECSGDLLVFLPGAGEIRRLQAQLGDLTGPRLQVLPMYGELDAREQDAALSEAPAGVR
                     RIVLATNIAETSVTIPGVTAVVDSGLARRARFDPVTGMSRLELGRISRAAADQRAGRA
                     GRTAPGRCYRLWSEGAHVGLAAFTPPEVSEADLAPLALELAQWGSVDASRLTWLEPPP
                     APMLAQARELLRRLEALDEHDAITPLGREMASLPLHPRLAHMLLAARRWSAVPLAADL
                     AALLSERDLLRRDGAERDPDLRTRLEILRRESTAAAADPGVLQRVRRSAAQFVQLAAS
                     AGASARRVGGGLRTEAGQRTETVPCSAGMLVAMAFPDRIAKRRGDGAGRFLLANGRGA
                     AFPRPAGLAGQEFLVCVELDDRDREARIDLAIPLEREELEEL"
BASE COUNT          247 a          722 c          762 g          296 t
ORIGIN      
        1 ggcaaaggca gcggccttgg ccttcgcatc gccaccgacc tcgtgctcgc gggcgtgctg
       61 ctgttctgcg ggagcctcta tgcgctgctg ggcggagcgc cacgcgcact gggcgctctg
      121 acgccgttcg gcggggtggc gttgatcgcc ggctggggcg tggcggcgat cgcactgctg
      181 cgcgcgcgcg atccctccgc gccgtgagcg caacgcagcc ggcagcgggc gcggcgctga
      241 tcgagccgct ccgcgcggcg ttcgccgcac atcgcgacgt cgtgctccgc gccccgcccg
      301 gcgccgggaa gagcacgctg gtaccactcg cgctgctcgc ggaaccgtgg ctgctcggcc
      361 ggaagatcct gctgctcgag ccacgacgcg tggcggcgcg tgcggtagcg cggcgcatgg
      421 cgtacctgcg cggcgagcaa gtcggtgaca ccgttggcta ccgcatgcgt ctcgacacgc
      481 gcgtgtcggg tcgcacgcgc atcgaggtca tcaccgaggg tgtgctgacg cgaatgctgc
      541 agtcggatcc ggaattggca ggtgtcggct gcgtcatctt cgacgagttc cacgagcgca
      601 gccttcaggc cgacctggcg ctcgcgctct gcctcgacgc gcgcaagaca ctcgatggtg
      661 acttccggat cctcgccatg tcggccactc tggacggcgc gcgggtcgct gctttgctgg
      721 gtggtgcgcc ggtcgtcgat gtcccgggtc gcgagttcga ggtgggagtc atctactgcg
      781 gccgcggagc gccgctgctg cccggcggca cggagccccc ggaacgtgcg ctcggccgca
      841 ccgtgcgacg cgcgctcgag gaatgcagcg gcgacctgct ggtgttcctg ccgggcgcgg
      901 gcgagatccg gcgactgcag gcgcagctcg gcgacctgac cggcccgcgg ctgcaggttc
      961 tgccgatgta cggcgagctc gatgcgcgcg agcaggacgc ggcgctcagc gaagctcccg
     1021 ccggcgtgcg acgcatagtg cttgccacca acatcgccga gaccagcgtg acgatcccgg
     1081 gcgtgacggc agtcgtcgat tcggggctcg cgcgccgcgc gcgcttcgat ccggtcaccg
     1141 gcatgagccg cctcgagctg gggcgcatct cgcgcgcggc ggcggaccag cgcgccggcc
     1201 gcgcgggccg gacagcgccg ggccgctgct accggctgtg gagcgaagga gcgcacgtgg
     1261 ggttggccgc gtttacgccg ccggaggtca gcgaggccga ccttgcaccg ctcgcgctcg
     1321 agctcgcgca gtggggcagc gtggatgcga gtcgtctcac gtggctcgag ccaccgcccg
     1381 cgccgatgct ggcgcaggcg cgcgagctgc tgcggcggct cgaggcactc gacgagcacg
     1441 acgcgatcac gccgctcggc cgcgagatgg cctcgctccc gctgcatccg cgcctggccc
     1501 acatgctgct cgccgcgcgc cgctggtcgg cggtgccgct ggccgcggac ctggcggcgc
     1561 tgctgtcgga acgtgacctg ctgcgccgtg acggcgccga gcgcgacccc gacctgcgca
     1621 cccggctcga gatcctgcgg cgcgagtcga ccgcagcggc ggccgacccg ggcgtgctgc
     1681 aacgggtgcg gcgcagtgcc gcgcagttcg tgcagctcgc agcatccgcg ggcgcgagtg
     1741 cacggcgcgt cggtggcggg ctgcgcaccg aagcagggca gcgcaccgaa actgtgcctt
     1801 gctccgctgg catgctcgtt gcgatggcct tccccgaccg catcgccaag cggcgtggcg
     1861 acggagccgg ccggttcctg cttgccaatg gacgcggcgc cgcctttccg cgccctgcgg
     1921 ggctcgccgg gcaggaattc ctggtatgcg tcgagctcga tgatcgcgat cgcgaggcac
     1981 gcatcgacct ggcgatcccg ctcgagcgcg aagagctcga agagctc
//
LOCUS       DASRDG010000422         5721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_295155, whole genome shotgun sequence.
ACCESSION   DASRDG010000422 DASRDG010000000
VERSION     DASRDG010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295155"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4814)
                     /locus_tag="VFA35_07005"
     CDS             complement(<1..4814)
                     /locus_tag="VFA35_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZT55957.1"
                     /translation="MQSLLSSVFETHANRAAVQGASIAWTYAELDRLSAGLAQALAER
                     GVAPGQAVPILMGRSPLLVLSQLALLRLGGSYAPIDMASPAPRRRAMLDALGSALALT
                     DGADAAEAAGSAEVFDVAAWLSGQRSGRAVRDPWVEPPPGTPACVMFTSGSTGVPKGV
                     MVPHAGIARLVHGANYAHFGPEQRWAQLSSPAFDASTLEVWGALLNGGCCMIQEEAVP
                     SLETLGEFLIAQRITDAWLTSALFNAMVEDQLAALGQLRQLLVGGERVSARHARLMLQ
                     AHPNVRLINGYGPTENTTFTLCHTITPDDTEGATGVPIGTPVAGTQVRVEPSDPDVPE
                     KGELWAAGDGVAFGYLGDAALTQAKFVWHGGARWYRTGDLVRRRSDGVFEFHGRIDRQ
                     IKLRGQRIELEEVELALAACPGVGNSAVLVVGQNADDRRLIAFYDGPEAADPSDAQVA
                     AHMRLTLPDAAIPSQFVRLQRLPANLSGKVDRKALEAMWVGGPASLVDRLQQVIALNP
                     DRPALEGRHETLSYAELDQRSARLAAQLIALGLAPGDHVALLLPRSVALVVAMLAVLR
                     AGAVYAPLDPGHPPQRTSRVLDLLKPKFTLTDGAAPGIAFGDCRCIDLAEPATLAAAR
                     GAAPWAVTPPDAPLYVMFTSGSTGVPKGVIVPGRGVLALVADAGAFGWAAFPPEARWL
                     LVTSPSFDISNLEIWGALLNGACCVIQEGELPSLDELARLITARRVTHAQLSTALFNA
                     MVDTQLGALLGLTQLITGGERASPPHMRKLLLARPGLRLINAYGPTETTIWSLSHRVD
                     VADTHAAAGIPIGRPVRGTRLRLEPLPIDAAEPTGSAELLIAGAGVALGYLHDAQQTR
                     RSFVELDGERWYRTGDLVSQRADGTLAFHGRADRQVKLQGQRIELDEVELALASCPGV
                     GEVAVLLHGDDAAHRHLVAFYAGLAGATPDAEQVTAHAAAQLPLAAVPTVVRALDQLP
                     QNRNGKVDRAALARLAEAADVPGAQDADAAAAPIGEFETRLAAIWQTLLPRARIGRGS
                     HFLRSGGSSLLALHVATQVHKQLGRNLAPVDVLRRPVLAEQAALISQLPQLGAEAAVY
                     DPSGGHRVPMTHLQQSLLAASAIDLTDCAYLVHVGLVLPEAPPDWLAWRAAFAALAQR
                     HPALRLSAFHDGEIARGTLEESLAPGWWQEHAPIAELPRDLDWPEAVQAAINRPLDTH
                     TQGSMRVDCWRVHGGGAAVVWTVHHHALDEASIGNALTELDALLRGAPLAPVYGSPFS
                     FQAVESAWADRPAAMGQWAARLAETLEGCTPPLERAPALGHERRFELGVDLRHALKAR
                     CAVLGCTPFTLLLTAYGLALQEAFGPRFRFVSTPFSRRAEPELLEPIGYLLDVRFVEA
                     GALPGEAPDATLARVHRAILDGQEPSFQWLDTLIEAVEAINPQAARCLSQFGFTWRLD
                     PARVVSMGGQTAQLLRVPQRGARLSMFLHAAQLGDTLGYSIEAVASAHRSGQVEAVAG
                     AFERHLAALCASPRDATLLARPAPATTAGAVLAAVDTALDATLTAAWARWLKLPEATV
                     TASSHFLRSGGSSLVAMRLAAELRREHGIKLDVGAFLGNATFAQLTALSRAKP"
     gene            complement(5019..>5721)
                     /locus_tag="VFA35_07010"
     CDS             complement(5019..>5721)
                     /locus_tag="VFA35_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZT55958.1"
                     /translation="FPQHPHRGFETVTIVYAGEVEHRDSAGGGGAIGPGDVQWMTAGA
                     GIVHEEYHGREFAKRGGAFEMIQLWVNLPAKDKMTPPHYQNITNARIPRVALADAAGS
                     VRVIAGEYAGTAGPARTFSPIELWDVRLNAGKRAEFKLPEGYTTAVFVLKGALQFGAD
                     TLREAELAVMERAGDTLAIEALEDTTLLLLGGQPLNEPVVGHGPFVMNTTAEIEQAFA
                     DYQSGRMGRIGVAAS"
BASE COUNT          814 a         2046 c         2085 g          776 t
ORIGIN      
        1 ggcttggccc gactcagcgc ggtcagctgc gcgaaggtcg cgttcccgag gaacgcaccg
       61 acgtcgagct tgatgccgtg ctcgcgccgc aactccgccg ccaagcgcat cgcgaccagc
      121 gacgaaccgc cgctgcgcag gaagtgactc gaggccgtca ccgtggcttc ggggagcttg
      181 agccagcgcg cccaggccgc ggtgagcgtg gcatcgagcg ccgtgtcgac ggccgcgagc
      241 acggcgcccg cagtcgttgc cggcgctggg cgggcgagca gcgtcgcgtc acgcggcgaa
      301 gcgcacagcg cggcgaggtg gcgctcgaac gcgcccgcga ccgcctcgac ctggccgctg
      361 cggtgcgccg atgcgaccgc ctcgatgctg tagccgagcg tgtcgccgag ctgcgccgcg
      421 tgcagaaaca tcgagagccg cgcgccccgc tgcggcacgc gcagcaactg ggcggtctgg
      481 ccgcccatcg acaccacccg cgccgggtcg agccgccagg tgaagccgaa ctggctcagg
      541 cagcgcgccg cctgcgggtt gatcgcctcg accgcttcga tcagcgtgtc gagccactgg
      601 aacgacggct cctggccatc gaggatcgcg cggtgcacgc gtgccagggt cgcgtcgggc
      661 gcctcgcctg gcagcgcgcc ggcctcgacg aaacgcacgt cgagcaggta gccgatcggc
      721 tcgagcaact cgggttcggc gcggcgtgag aacggcgtcg agacgaagcg aaaccgcggg
      781 ccgaacgcct cctgcagtgc caagccatag gccgtcagca gcagcgtgaa cggcgtgcac
      841 ccgagcaccg cgcaacgcgc cttcagcgcg tgtcgcagat cgacgccgag ctcgaagcgg
      901 cgttcgtggc cgagcgctgg tgcacgctcg agcggcggcg tgcagccctc gagcgtctcg
      961 gccagccgcg ccgcccactg ccccatagcc gccggccggt cggcccaggc tgattcgacc
     1021 gcctggaacg agaacggcga gccgtacacc ggtgccagcg gcgcgccgcg cagcagcgcg
     1081 tcgagttcgg tgagggcgtt gccgatgctg gcttcgtcga gcgcgtggtg atgcaccgtc
     1141 cagacgactg cggcgccacc gccgtgcacg cgccagcagt cgacgcgcat gctgccttgg
     1201 gtatgcgtgt cgagcggtcg attgatcgcg gcctgaaccg cctcgggcca gtccaggtcg
     1261 cgcggcagct cggcgatcgg cgcgtgctcc tgccaccagc cgggggcgag gctttcctcc
     1321 agcgtgccgc gcgcgatttc gccgtcgtga aacgccgaga gccgcagcgc cggatggcgt
     1381 tgcgcaaggg ccgcgaaggc cgcgcgccag gccaaccagt cgggcggcgc ctcgggcagc
     1441 accaggccga catgcaccag gtacgcacag tcggtcaggt cgatcgcgct ggcggccagc
     1501 aggctttgct gcaggtgggt catcggcacg cggtggccgc cgctcgggtc gtacaccgcc
     1561 gcctcggcac cgagttgcgg cagttgcgag atcagcgcgg cctgctcggc gagcaccggg
     1621 cggcgcagca cgtcgaccgg cgcgaggttg cgcccgagtt gcttgtgaac ctgggtcgcg
     1681 acatgcagtg cgagcagcga cgagccgccg ctgcgcagga agtgcgagcc gcggccgatc
     1741 cgcgcgcgcg gcagcagcgt ctgccagatc gccgcgagcc gggtctcgaa ttcgccgatc
     1801 ggcgcggccg cggcgtcggc gtcctgcgcg ccgggcacgt cggcggcctc ggcgagccgg
     1861 gcgagggcgg cgcgatcgac cttgccgttg cggttctgcg gcagttggtc gagcgcgcgg
     1921 acgaccgtcg gcacggccgc cagcggcagc tgcgcggcgg catgcgcggt tacctgctcg
     1981 gcatccggcg tcgcgccggc caggccggca tagaacgcca ccaggtggcg gtgcgccgcg
     2041 tcgtcgccgt gcagcaacac cgcgacctcg ccgacaccgg ggcagctcgc gagcgcgagc
     2101 tcgacctcgt cgagctcgat gcgctggccc tgcagcttga cctgccggtc ggcacggccg
     2161 tggaaggcca gcgtgccgtc ggcgcgttgc gaaaccagat cgcccgtgcg gtaccagcgc
     2221 tcgccgtcga gctcgacgaa gctgcggcgt gtctgctggg catcgtgcag atagccgagc
     2281 gcgacgccgg cgccggcgat cagcagctcg gccgacccgg ttggctcggc cgcatcgatc
     2341 ggcagcggct ccaagcgcag ccgcgtgccg cgcacgggcc ggccgatcgg gatgccggcg
     2401 gcagcgtgcg tgtcggccac gtcgacccgg tgcgacaggc tccagatcgt cgtctcggtc
     2461 gggccgtacg cgttgatcag gcgcaggccg gggcgtgcca gcagcagctt gcgcatgtgc
     2521 ggcggcgacg cgcgctcgcc gccggtgatc agctgcgtca ggccgagcag ggcgccgagc
     2581 tgggtgtcga ccatcgcgtt gaacagcgcc gtcgagagct gggcgtgcgt gacgcggcgc
     2641 gccgtgatca ggcgcgcgag ttcatcgagc gaaggcaact cgccctcctg gatcacgcag
     2701 caggcgccgt tcagcagcgc gccccagatc tcgaggttgc tgatgtcgaa cgagggcgag
     2761 gtgacgagca gccagcgcgc ctccggcggg aacgcggccc agccgaaggc accggcatcg
     2821 gcgaccagcg ccagcacgcc gcggcccggc acgatcacgc ccttgggcac gccggtggag
     2881 cccgacgtga acatcacgta gagcggggcg tcgggtggcg tgacggccca gggcgcggcg
     2941 ccgcgcgccg cggcgagcgt ggccggctcg gcgagatcga tgcagcggca gtccccgaac
     3001 gcgatgcccg gcgccgcgcc gtcggtcagc gtgaacttcg gcttcagcag gtcgagcacg
     3061 cgcgacgtgc gttgcggcgg atgccccggg tcgagcggcg catagaccgc gccggcacgc
     3121 agcaccgcga gcatcgcgac cactagcgcg accgatcgcg gcagcagcag cgcgacgtgg
     3181 tcgcccggcg ccagcccgag cgcgatcagc tgcgccgcca gccgcgcact gcgctggtcc
     3241 agctcggcgt agctcagcgt ctcgtggcga ccctcgagcg cggggcggtc cgggttcaaa
     3301 gcgatgacct gttgcagtcg atcgaccagc gaggccggtc cgccgaccca catcgcttcg
     3361 agcgctttgc ggtcgacctt gccgctcaag ttggccggca atcgctgcag ccgcacgaac
     3421 tgcgacggga tcgcggcgtc gggcagcgtc aggcgcatgt gcgcggcgac ctgcgcatcg
     3481 ctcgggtcgg cggcctcggg cccgtcgtag aacgcgatga ggcggcgatc gtcggcgttc
     3541 tgcccgacga caagcaccgc gctgttgccg acccccgggc acgccgccag cgcgagttcg
     3601 acctcttcga gctcgatgcg ctggccgcgc agcttgatct gccggtcgat gcggccgtga
     3661 aactcgaaca cgccgtcgct gcgtcgccgc accaggtcgc cggtccgata ccagcgcgcg
     3721 ccgccgtgcc agacgaactt ggcctgcgtc agcgcggcat cgccaaggta gccgaatgcg
     3781 acgccgtcgc cggcggccca gagttcgccc ttctcgggta catcgggatc gctcggctcc
     3841 acgcgcacct gtgtacccgc caccggcgtg ccgatcggca cgccggtggc gccctcggtg
     3901 tcatccggcg tgatcgtgtg gcacagcgtg aaggtggtgt tctcggtcgg cccgtagccg
     3961 ttgatcaggc gcacgttcgg gtgcgcctgc agcatcaggc gcgcgtgccg cgccgagacc
     4021 cgctcgccgc ccaccagcaa ctggcgcagt tgcccgagcg cggcgagctg gtcttcgacc
     4081 atcgcgttga acagcgccga ggtcagccag gcgtcggtga tgcgctgcgc gatcaggaac
     4141 tcgcccagcg tctcgagcga cggcacggcc tcttcctgga tcatgcagca gccgccgttc
     4201 agcagcgcgc cccagacctc gagcgtcgag gcgtcgaacg ccggcgacga gagctgggcc
     4261 cagcgctgct ccgggccgaa gtgcgcgtag ttcgcgccgt gcacgagccg cgcgatgccg
     4321 gcgtgcggca ccatcacgcc cttgggcacg ccggtcgaac ctgacgtgaa catcacgcag
     4381 gccggcgtgc cgggcggggg ttcgacccac gggtcgcgca ccgcgcggcc agagcgttga
     4441 cccgacagcc aggccgcgac atcgaagacc tccgcgctgc cggccgcctc ggcggcgtcc
     4501 gcaccgtcgg tcagcgccag cgcgctgccg agcgcatcga gcatcgcgcg ccggcgcggc
     4561 gcggggctcg ccatgtcgat cggcgcgtag ctgccgccca ggcgcagcag cgcgagttgc
     4621 gacagcacga gcaggggcga gcggcccatc aggatcggca cggcctggcc cggcgccacc
     4681 ccgcgttcgg ccagagcctg cgccaggccg gcgctcaagc gatcgagttc ggcgtaggtc
     4741 cacgcgatcg atgcgccctg caccgccgcg cggttcgcgt gtgtttcgaa cacgctgctc
     4801 aacagcgatt gcatcgaagg gatagacatg ccttgcagag attcgggccg gatgcgagtg
     4861 ccgcaccgga cgcaaggtca ttgtaggaat ccgcgtcgcc gcggcagtgc gcattgcgcc
     4921 cgcgaatcgg ggggtgcgcg acgtttcaga gggaaaaagt tggggcgcct tgtgcgatct
     4981 gccacggtct gcatgttcgc gccgcgccca gcggctccct atgaggcggc gacgccgatc
     5041 cgccccatgc gaccgctttg gtagtccgcg aaggcctgct cgatttcggc ggtcgtgttc
     5101 atgacgaacg gaccgtggcc gaccaccggc tcgttcaacg gctggccgcc cagcagcaac
     5161 agcgtcgtgt cttcgagtgc ttcgatggcc agcgtgtcgc cggcgcgctc catcacggcg
     5221 agttcggcct cgcgcagcgt gtcggcgccg aactgcaaag cacccttgag cacgaagacc
     5281 gcggtcgtgt agccctccgg cagtttgaat tccgcccgct tgccggcgtt cagccgaaca
     5341 tcccagagct cgatggggct gaaggtccgc gccggccccg ccgttcccgc gtattcgccg
     5401 gcgatcacgc ggaccgagcc ggcggcgtcc gcgagcgcaa cgcgcgggat ccgcgcgttc
     5461 gtgatgttct gatagtgcgg cggcgtcatc ttgtccttcg cgggcaggtt gacccacagc
     5521 tgaatcatct cgaacgcgcc gccgcgcttg gcgaactcgc ggccgtggta ctcctcgtgc
     5581 acgatgccgg cgccggcggt catccattgg acgtcgcccg gcccgatggc gcccccgccc
     5641 ccggcggagt cgcgatgctc gacctcgccg gcgtagacga tggtcaccgt ctcgaagccg
     5701 cggtgcggat gctgcggaaa g
//
LOCUS       DASRDG010000423         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_424396, whole genome shotgun sequence.
ACCESSION   DASRDG010000423 DASRDG010000000
VERSION     DASRDG010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424396"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /gene="pgi"
                     /locus_tag="VFA35_07015"
     CDS             <1..1040
                     /gene="pgi"
                     /locus_tag="VFA35_07015"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZT55959.1"
                     /translation="TFTTQETMANAHAAKDWFEANGGTDIAKHFCATTTNVETAAQFG
                     ITTTFGFWDWVGGRYSLWSAIGLPIALAIGADNFRALLAGAHAMDEHFAHAPAAKNLP
                     LVLALIDVWYRNFHGFTSRCMAPYHQGLKRLPAYLQQLEMESNGKRVDLDGRVVPYAT
                     SPVVWGEPGTNGQHAYYQMLHQGTDVIPVEFILVKNVTHDMSNLHGPLLAQLERQNTM
                     LLANGLAQSKALMQGKTTAKAAAETAPPTASEEIDAATLAAHRTFPGNRPSTTLVLDR
                     LTPRSVGALIALYEHRVFASGALWGLNSFDQWGVELGKALANQLLPRLDSGDTSGLDA
                     STAGLVKRLRG"
     gene            1051..1665
                     /locus_tag="VFA35_07020"
     CDS             1051..1665
                     /locus_tag="VFA35_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZT55960.1"
                     /translation="MNIVFDFGGVLLHWQPHEIIARWLPQRAPDPATARALAAAFFQG
                     VGGAWGEFDRGTLDRGPLAEGIAGRTGLTRAEVDTVIDAVPHELQPIEATVALLTRLQ
                     ARGHALYFLSNMAAPYADHLEATHDFIGLFRAGVFSARVKMIKPEAAIFAHAVEAFGI
                     DPADTLFIDDLLHNAEAARAAGWQALHFEDAAQCEAELARRGLL"
     gene            complement(1670..2227)
                     /locus_tag="VFA35_07025"
     CDS             complement(1670..2227)
                     /locus_tag="VFA35_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZT55961.1"
                     /translation="MHTALILIDVQESFRQRPYWVEAPAQGFLARANALVEGCAARSV
                     PIVRILHSDGPAVASNPFAIESGFVRPLAGVAPFDAAAEFVKHRHSALVGTGLTVWLH
                     RNRIARLIVAGIRTEQCCETTARHASDEGFEVDYVAEATLTFDMRHLDGTPLPAADIV
                     ARTTAVLTDRFATICSVAQALERAA"
     gene            complement(2242..>2600)
                     /locus_tag="VFA35_07030"
     CDS             complement(2242..>2600)
                     /locus_tag="VFA35_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HZT55962.1"
                     /translation="LARARAWWGDKVTSIYDGSSTSAPLQGLMWLAAYQECAQAEYTG
                     IALEYGTLAVMEMIDALRADQWLENHPEAPAALRTQIKQQMRDAFYTDTDAWKQRVVE
                     QALDAAHGALRGLAAA"
BASE COUNT          392 a          908 c          927 g          373 t
ORIGIN      
        1 agaccttcac gacgcaggag acgatggcca acgcgcacgc cgcgaaggac tggttcgagg
       61 cgaatggcgg caccgacatc gccaagcact tctgcgcgac cacgaccaac gtcgagaccg
      121 ccgcgcagtt cggcatcacg acgaccttcg gcttctggga ctgggtcggc ggccgctact
      181 cgctgtggtc ggcgatcggg ctgccgatcg cgctggcgat cggcgccgac aacttccgcg
      241 cgctgcttgc cggcgcacac gcgatggacg agcacttcgc gcacgcgccc gccgcgaaga
      301 acctgccctt ggtgctcgcg ctgatcgacg tctggtaccg caacttccac gggttcacga
      361 gccgctgcat ggcgccgtac caccagggcc tgaaacgcct gcccgcctac ctgcagcagc
      421 tcgagatgga gagcaacggc aagcgcgtcg atctcgacgg ccgcgtcgtg ccgtacgcga
      481 ccagcccggt ggtgtggggc gagccgggca cgaacggcca gcacgcctac taccagatgc
      541 tgcaccaggg caccgacgtg atcccggtcg agttcatcct ggtgaagaac gtgacgcacg
      601 acatgagcaa cctgcacggc ccgctgctcg cgcaactcga acgccagaac acgatgctgc
      661 tcgcgaacgg cctggcgcaa tcgaaggcgc tgatgcaagg caagacgacc gcgaaggccg
      721 cggccgagac ggcgccgccg accgcgtcgg aggagatcga cgcggcgaca ctcgcggcgc
      781 accgcacctt ccccggcaac cggccgagca cgaccctcgt gctggaccgg ctcacgccgc
      841 gctcggtcgg cgcgctgatc gcgctgtacg aacaccgcgt gttcgcgagc ggcgcgctgt
      901 ggggcctgaa cagcttcgac cagtggggcg tcgagctcgg caaggcgctc gcgaaccaat
      961 tgctgccgcg gctcgactcc ggcgatacga gcgggctcga cgcgtcgacg gcggggctgg
     1021 tgaagcggct gcgcgggtga cgcccggcgc gtgaacatcg ttttcgattt cggcggcgtg
     1081 ctgctgcact ggcagccgca cgagatcatc gcgcgctggc tgccgcagcg tgcgcccgac
     1141 cccgcgacgg cgcgtgcgct ggcggcggcg ttctttcaag gtgtcggcgg cgcgtggggc
     1201 gagttcgacc gcggcacgct cgatcgcggg ccgctggccg agggcatcgc cgggcgcacc
     1261 ggcctgaccc gcgccgaggt cgacaccgtg atcgacgcgg tgccgcacga actgcagccg
     1321 atcgaggcga ccgtcgcgtt gctgacgcgg ctgcaggcgc gcggccacgc gctgtacttc
     1381 ctgtcgaaca tggcggcgcc atatgccgac cacctcgaag ccacgcatga cttcatcggc
     1441 ctgttccggg ccggcgtgtt ctcggcgcgc gtcaagatga tcaagcccga ggctgcgatc
     1501 ttcgcgcacg cggtcgaggc gttcggcatc gacccggccg acacgctgtt catcgacgac
     1561 ctgctgcaca acgccgaggc ggcgcgcgcc gccggctggc aggcgctgca cttcgaggat
     1621 gcggcgcagt gcgaggccga gctcgcgcgg cgcggcctgc tgtgacgctt caggccgcgc
     1681 gctcgagcgc ctgcgccacg ctgcagatcg tcgcgaagcg gtccgtcaac accgcggtcg
     1741 tgcgcgcgac gatgtcggcg gccggcagcg gcgtaccgtc gaggtggcgc atgtcgaagg
     1801 tcagcgtggc ctcggcgacg tagtcgacct cgaagccttc gtcgctggcg tggcgcgcgg
     1861 tcgtctcgca gcactgctcg gtgcggatgc cggcgacgat cagccgggcg atgcggttgc
     1921 ggtgcagcca gaccgtcagc ccggtgccca ccagcgcgct gtggcggtgc ttgacgaact
     1981 cggccgcggc gtcgaacggc gccacgccgg ccagcgggcg gacgaagccg gactcgatcg
     2041 cgaacgggtt cgacgccacg gccggcccgt cgctgtgcag gatgcgcacg atcggaacgg
     2101 agcgcgccgc gcaaccctcg accaacgcgt tcgcgcgtgc gagaaaaccc tgcgccggcg
     2161 cttcgaccca gtaggggcgt tgacgaaagg actcttgcac gtcgatcagg atcagggcgg
     2221 tgtgcatcgc ggtgctcggt ctcaggcggc cgcgaggccg cgcagcgcgc cgtgcgccgc
     2281 gtccagcgcc tgctcgacga cgcgctgctt ccacgcgtcg gtgtcagtgt agaaggcgtc
     2341 gcgcatctgc tgcttgatct gcgtgcgcag cgccgccggt gcctcgggat ggttttcgag
     2401 ccactggtcg gcgcgcagcg cgtcgatcat ttccatgacg gccaaggtac cgtattcgag
     2461 cgcgatgccg gtgtattccg cctgcgcgca ctcctggtag gcggcgagcc acatcaggcc
     2521 ctgcagcggc gccgaggtcg agctgccgtc gtagatgctg gtgaccttgt cgccccacca
     2581 ggcgcgggcg cgcgcgagcg
//
LOCUS       DASRDG010000424         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_519076, whole genome shotgun sequence.
ACCESSION   DASRDG010000424 DASRDG010000000
VERSION     DASRDG010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519076"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2027
                     /locus_tag="VFA35_07035"
     CDS             <1..2027
                     /locus_tag="VFA35_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT55963.1"
                     /translation="QGRIELAQSRDPALQAHTGQALTGIVGGATSITLNDTLALQRSP
                     RWIIDDAERAHQQGLQEQLARALAEPEVRLLFADGGAAELRPAPRGLSGLPILFWPLS
                     IFALGLYLVAMVVLLAHPTGRNLVYGVMALCQSGNLLFIAVEATLDAGLPAAPFARLD
                     MPVRMALDLFTAAAMVNAACLHPRRLPGSGWIALFGWGVAFALVGALAAGALGNVWWW
                     TQGTVIGLGLLAIGLLSWSYQIEPHPFAIVLRRFGIVTVATWTLLTVALAAADRLPGM
                     PHNFADIGSTVWYVFLGSLLMLVPFLSKSQNFMREFSLLAAISTVATSLDLLFVAVFS
                     LGQFASLTLSLFVSLAVYTGVRQWVLNQLRGDSVITTERMFEQLYRVAREVEARPERM
                     PALLSQLLGDLFEPLEVGVAERRGGRTRVAGDGSSMLVPVPLLGGDAAPRGAILIRFA
                     QRGRRLFTAEDARLTDRIVEQLRRAVHFDKAVEQGRSEERLRLAQDLHDDIGARLLTL
                     MYKAQSPEMEDYVRHTLQDLKTLTRGLAASNHRLSHAAAEWKSDLAHRLTAAHVDLKW
                     TFAFDEDILLTVVHWSALTRVMRELVSNVITHSQAQELEIDFRLEADRIELTITDDGI
                     GRNPRAWSHGLGLGGVRKRVKQLGGEVEWREVTPHGISCRVIIRDLSARW"
     gene            complement(2125..2200)
                     /locus_tag="VFA35_07040"
     tRNA            complement(2125..2200)
                     /locus_tag="VFA35_07040"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2165..2167),aa:His,seq:gtg)
     gene            complement(2254..>2608)
                     /locus_tag="VFA35_07045"
     CDS             complement(2254..>2608)
                     /locus_tag="VFA35_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZT55964.1"
                     /translation="ATAMSEAAVTFSRAQARDLPGALVDAVLKAPAATLPAFVGVDLG
                     DQGYAVVKIGKVLGRDPVTADASRAQAQYAQAWAEAESQAYYNALKTRFKVEIKPNAL
                     PAAADAASAASAATK"
BASE COUNT          353 a          910 c          912 g          433 t
ORIGIN      
        1 cgcaagggcg catcgagctc gcgcagagcc gcgatccggc gctgcaggcg cacaccggcc
       61 aggcactgac cggcatcgtc ggcggcgcca ccagcatcac cctgaacgac acgctcgcgc
      121 tgcagcgctc gccgcgctgg atcatcgacg acgccgagcg cgcgcaccag caggggctgc
      181 aagagcagct cgcgcgtgcg ctcgccgaac ccgaggtccg gctgctcttc gccgacggcg
      241 gggcggccga gctccggccg gcgccgcgcg ggctgagcgg gctgccgatc ctgttctggc
      301 cgctgtcgat cttcgcgctc gggctgtacc tggtggcgat ggtcgtgctg ctcgcgcacc
      361 cgaccgggcg caacctcgtg tacggggtga tggcgctgtg ccagtccggc aacctgctgt
      421 tcatcgcggt cgaggcgacg ctcgacgccg gcctgcccgc ggcgccgttc gcgcggctcg
      481 acatgccggt gcgcatggcc ctcgacctgt tcaccgccgc ggcgatggtc aacgcggcct
      541 gtctgcatcc gcggcgtttg ccgggctccg gctggatcgc gttgttcggc tggggcgtgg
      601 cgttcgcgct cgtcggcgcg ctggcggcag gcgcgctcgg caacgtctgg tggtggacgc
      661 agggcacggt gatcggcctg ggattgctcg cgatcggcct gctgagctgg tcgtaccaga
      721 tcgaaccgca tccgttcgcg atcgtgctgc ggcgcttcgg catcgtcacc gtggcgacct
      781 ggacgctgct gaccgtcgcg cttgccgccg ccgaccgcct gcccggcatg ccgcacaact
      841 tcgccgacat cggctcgacg gtctggtatg tgtttctcgg ctcgctgctg atgctcgtgc
      901 cgtttctgtc gaagtcgcag aacttcatgc gcgagttctc gctgctggcg gcgatcagca
      961 cggttgcgac ctcgctcgac ttgctgttcg tcgcagtgtt ctcgctcggg cagttcgcct
     1021 cgctgacctt gtcgctgttc gtctcgctcg cggtctacac cggcgtgcgc cagtgggtgc
     1081 tgaaccagct gcgcggcgac agcgtgatca cgaccgagcg catgttcgag cagctgtacc
     1141 gcgtcgcgcg cgaggtcgaa gcccggccgg agcgcatgcc cgcgctgctg tcgcaactgc
     1201 tcggcgatct gttcgagccg ctcgaggtcg gcgtcgccga gcgtcgcggc ggccgcacac
     1261 gcgtcgccgg cgacggctcc agcatgctgg tgcccgtgcc gctgctcggc ggcgacgccg
     1321 cgccgcgcgg tgccattctg atccgcttcg cgcagcgcgg ccggcgcctg ttcaccgccg
     1381 aagacgcgcg cctgaccgat cgcatcgtcg agcagctgcg ccgcgcggtg cacttcgaca
     1441 aggcggtcga gcaaggccgc agcgaagagc ggctgcgcct ggcgcaagac ctgcacgacg
     1501 acatcggcgc gcgcttgctg acgctgatgt acaaggccca gtcgcccgag atggaagact
     1561 atgtgcgcca cacgctgcag gacctgaaga cgctgacgcg cggtctcgcc gcctcgaatc
     1621 accggctctc gcacgccgcc gcggagtgga agtccgacct cgcgcaccgg ctcaccgccg
     1681 cgcacgtcga cctgaagtgg accttcgcgt tcgacgaaga catcctgctc accgtcgtgc
     1741 actggtcggc gctgacgcgc gtgatgcgcg aactcgtcag caacgtgatc acgcattcgc
     1801 aggcgcagga actcgagatc gacttccgcc tcgaggccga ccggatcgaa ctgacgatca
     1861 ccgacgacgg catcggccgc aacccgcgcg cctggtcgca cgggctcggg ctgggcggcg
     1921 tgcgcaagcg cgtgaagcaa ctcggcggcg aggtcgaatg gcgcgaggtc acgccgcacg
     1981 gcatcagctg ccgcgtgatc atcagggacc tgtcggcgcg gtggtgaggc gcctgtggcg
     2041 gacatgaaaa agccgggcgc ttggcccggc tttcgtgttc gccctcggcc gcgtcattgg
     2101 cggcgtcagc tggcgttgat tctttggggt ggctgatggg gctcgaaccc acgacaacag
     2161 gaatcacaat cctggactct accaactgag ctacagccac cgtagaacac gtgattataa
     2221 cggaagcgcc gtgcgctttc gttcgtggcc cggctacttc gtcgccgcgc tcgcagcgct
     2281 ggccgcgtcg gcagcggccg gcagcgcgtt cggtttgatc tcgaccttga agcgcgtctt
     2341 cagcgcgttg tagtaggcct gcgactcggc ctcggcccag gcttgcgcgt actgcgcctg
     2401 cgcgcgcgag gcgtcggccg tgaccgggtc gcggcccagc accttgccga tcttgacgac
     2461 ggcgtagccc tggtcgccga gatcgacgcc gacgaaggcg ggcagcgtcg cggccggcgc
     2521 cttcagcacc gcatcgacga gcgcgccggg cagatcgcgg gcttgagcgc gcgagaaggt
     2581 cacggcggcc tcgctcatcg cggtggcg
//
LOCUS       DASRDG010000425         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_425010, whole genome shotgun sequence.
ACCESSION   DASRDG010000425 DASRDG010000000
VERSION     DASRDG010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425010"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VFA35_07050"
     CDS             <1..698
                     /locus_tag="VFA35_07050"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZT55965.1"
                     /translation="GYRAVTLSLKRAGQAPGDATADQLDAAAAIADRFSHGELRVTHD
                     QNLLLPWVRATELHALWQAARDASFATPNIGLLTDMIACPGGDFCALANARSIPIAEE
                     ITQRFAEIDELYDIGDIDLHISGCINSCGHHHSGHIGILGVDKDGKEWYQVSLGGSDG
                     STISGASVPGKVIGPSFAADEVADVIESVVETYRNQRVANELFIDTVKRVGLDPFKAS
                     ANAVRRSTATARA"
     gene            710..1255
                     /locus_tag="VFA35_07055"
     CDS             710..1255
                     /locus_tag="VFA35_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HZT55966.1"
                     /translation="MKFINPQKDPWHTLGGEDGPPAYIDPRPHLLLTLEQWHAFRAQW
                     PEGMPVGVMLPNDTEVKEIAADLPRLALVQLVFPKWMDGRAYSQAHTLRMRYRYRGEV
                     RASGEALVDMVLLMARTGFDAVVLRADQSLDAAHRALTFFPGYYQGSADDPLPLFAKP
                     PGTGDALAKAQEEFLHAGASI"
     gene            1252..2004
                     /locus_tag="VFA35_07060"
     CDS             1252..2004
                     /locus_tag="VFA35_07060"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZT55967.1"
                     /translation="MSAITLYARETPGFESRVASALALLRGAAAEHPGRIVQATSLGA
                     EDMVVTDLIARNKLPIAIGTLETGMLHAETVGLIRQIESRYGVEVEVFEPVRESVVHF
                     VRKNGERAMYDSIELRKACCAIRKLEPLSRMLEGRSAWVTGMRREQSANRGTVEFTEL
                     DDKGRTKVNPLADWSWADVWQYIARNDVPYNPLHDQFYPSIGCAPCTRAIAVGEDFRA
                     GRWWWEDEGAKECGLHVHEEPAQTVSMIGAHP"
     gene            2001..2960
                     /gene="cysD"
                     /locus_tag="VFA35_07065"
     CDS             2001..2960
                     /gene="cysD"
                     /locus_tag="VFA35_07065"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HZT55968.1"
                     /translation="MNAPLTVEQLLPALDHTHLDWLEEEAIFILRETVAAFERPALLF
                     SGGKDSCVVLRLAEKAFKKKNAQGEYEGRLPFPLLHVDTGHNFPEVIEFRDRRVAEMG
                     ERLVVGHLEDSIARGTVRLSHPLESRNGHQTVALLEAIEEHRFDVLIGGARRDEEKAR
                     AKERIFSHRDSFGQWQPKEQRPELWTLFNTRIKPGEHMRAFPISNWTELDVWLYIARE
                     NIPLPNLYFAHDREVIRRKGLLVPLTDVTPALPGESVETVEVRFRTVGDMTCTCPVES
                     PAADAAQIVAETLQVTISERGATRMDDRTSEASMERRKKEGYF"
     gene            2960..>4188
                     /locus_tag="VFA35_07070"
     CDS             2960..>4188
                     /locus_tag="VFA35_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZT55969.1"
                     /translation="MSDHREGQKGADHRALRFLTAGSVDDGKSTLIGRLLYDSRAILA
                     DQLDTLEKRAAGAPIDLSLLTDGLEAEREQGITIDVAYRYFATRQRKFIIADAPGHEQ
                     YTRNMVTAAAGSDAAVVLVDITKLDTHALPVKLLAQTRRHALLAQLLRVPSIVFAINK
                     LDAVDDPAQAYEAVRAALLDFAAEAKMEVAGIIPVSALRGDNVTQPPAAANATLGAWA
                     SWYQGPSLLQLLESLPTAQERTVGDLLIPVQYVARDSDGVGDKPRTLWGRIAHGRVQA
                     GDEVQLFPSRQKAVVAEVRVAGELVDAAEAGQSAGLSLDRQLDVSRGDWIATPDTVHE
                     TNRFSATLAWLDTEPAVIGRKYSVRHGNRWVLARITAIESRLDIHSLQATDAHELAVN
                     EIGHVVIETQQPLPVEA"
BASE COUNT          717 a         1495 c         1412 g          564 t
ORIGIN      
        1 ccggctaccg cgccgtgacg ctctcgctga agcgtgccgg ccaggcgccc ggcgacgcca
       61 ccgccgacca gctcgacgcg gccgccgcga tcgccgaccg cttcagccat ggcgagctgc
      121 gtgtcacgca cgaccagaac ctgctgctgc cgtgggtgcg cgcgaccgag ctgcacgcgc
      181 tctggcaggc cgcgcgcgac gccagcttcg cgacacccaa catcggcctg ctgaccgaca
      241 tgatcgcgtg ccccggcggc gacttctgcg cgctcgcgaa cgcgcgctcg atcccgatcg
      301 ccgaagagat cacgcagcgc ttcgccgaga tcgacgagct ctacgacatc ggcgacatcg
      361 acctgcacat cagtggctgc atcaactcct gcggccacca ccacagcggc cacatcggca
      421 tcctcggcgt cgacaaggac ggcaaggagt ggtaccaggt ctcgctcggc ggctcggacg
      481 gctcgaccat cagcggcgcc tcggtgcccg gcaaggtgat cgggccgtcg ttcgcggccg
      541 acgaggtggc cgacgtgatc gagtcggtgg tcgagaccta ccgcaaccag cgcgtggcga
      601 acgagttgtt catcgacacc gtcaagcgcg tcggtctcga cccgttcaag gcctcggcga
      661 atgccgtgcg ccgcagcacc gcgacggcgc gcgcctgagg agcatcacca tgaagttcat
      721 caacccccag aaagaccctt ggcacacgct cggcggtgaa gacgggccgc ccgcctacat
      781 cgacccgcgc ccgcacctgc tgctgacgct cgagcaatgg cacgcgttcc gcgcgcagtg
      841 gcccgaaggc atgccggtcg gcgtgatgct cccgaacgac accgaggtca aggagatcgc
      901 cgccgacctg ccgcgcctcg cgctcgtgca gctcgtcttc ccgaagtgga tggacggccg
      961 cgcctacagc caggcccata cgctgcgcat gcgctaccgc taccggggcg aggtgcgcgc
     1021 gagcggcgag gcgctggtcg acatggtgct gctgatggcg cgcaccggct tcgacgcggt
     1081 cgtgctgcgc gccgaccagt cgctcgacgc cgcgcaccgc gcgctgacct tctttcccgg
     1141 ctactaccaa ggcagcgccg acgacccgct gccgctgttc gcgaagccgc ccggcaccgg
     1201 cgacgcgctg gccaaggcgc aagaggagtt tctgcacgcg ggggcttcga tatgagcgcg
     1261 atcacgctct atgcgcgcga gacgccgggc ttcgagagcc gcgtcgccag cgcgctcgcg
     1321 ctgctgcggg gtgccgccgc agagcacccg gggcgcatcg tgcaggcgac cagcctgggc
     1381 gccgaagaca tggtggtcac cgacctgatc gcgcgcaaca agctgccgat cgcgatcggc
     1441 acgctcgaaa ccggcatgct gcacgccgag acggtcggac tgatccggca gatcgaatcg
     1501 cgttatggcg tcgaggtcga agtgttcgag ccggtccggg agtcggtcgt gcacttcgtc
     1561 cgcaagaacg gcgagcgcgc gatgtacgac agcatcgagc tgcgcaaggc ctgctgcgcg
     1621 atccgcaagc tcgagccgct gtcgcgcatg ctcgagggcc gcagcgcctg ggtcaccggc
     1681 atgcgccgcg aacagtcggc gaaccgcggc acggtggagt tcaccgagct cgacgacaaa
     1741 ggccgcacca aggtcaaccc gctcgccgac tggagctggg ccgatgtatg gcagtacatc
     1801 gcgcgcaacg acgtgcccta caacccgctg cacgaccagt tctacccgag catcggctgc
     1861 gcgccgtgca cgcgcgcgat cgcggtcggc gaggacttcc gcgccggccg ctggtggtgg
     1921 gaagacgaag gcgcgaaaga gtgcggcctg cacgtgcatg aagaacccgc ccaaaccgtt
     1981 tcgatgatcg gagcccatcc atgaatgcgc cgttgacggt cgagcaactg ctgcccgcac
     2041 tcgaccacac ccacctcgac tggctcgagg aagaagcgat cttcatcctg cgcgagaccg
     2101 tcgcggcgtt cgagcggccg gcgctgctgt tctcgggcgg caaggattcg tgcgtcgtgc
     2161 tgcgcctggc cgagaaggct ttcaagaaga agaacgcgca gggcgagtac gaaggccgcc
     2221 tgccgttccc gctgctgcac gtggacaccg gccacaactt ccccgaggtg atcgagttcc
     2281 gcgaccggcg cgtcgccgag atgggcgagc ggctcgtcgt cggccacctc gaggactcga
     2341 tcgcgcgcgg cacggtgcgc ctgagccacc cgctcgaatc gcgcaacggc caccagaccg
     2401 tcgcgctgct cgaagcgatc gaggagcacc gtttcgacgt gctgatcggc ggcgcgcgcc
     2461 gcgacgaaga aaaggcccgc gcgaaggagc gcatcttctc gcaccgcgac agcttcggcc
     2521 agtggcagcc gaaggagcag cgccccgaac tgtggacgct gttcaacacc cgcatcaagc
     2581 ccggcgagca catgcgcgcg ttcccgatca gcaactggac cgagctcgac gtctggctct
     2641 acatcgcgcg cgagaacatc ccgctgccga acctgtactt tgcgcacgac cgcgaggtga
     2701 tccgccgcaa gggcctgctg gtgccgctga ccgacgtgac gccggcgctg ccgggcgaat
     2761 cggtcgagac ggtcgaggtg cgctttcgca ccgtgggcga catgacctgc acctgcccgg
     2821 tcgagagccc ggcggccgac gcggcccaga tcgtcgccga aacgctgcag gtgaccatca
     2881 gcgaacgcgg cgccacccgc atggacgacc gcacctccga ggcctcgatg gagcggcgaa
     2941 agaaggaagg gtatttctga tgagtgacca ccgcgaaggc cagaagggcg cggaccaccg
     3001 cgccttgcgc ttcctcaccg ccggcagcgt cgacgacggc aagagcacgc tgatcggccg
     3061 cctgctgtac gactcgcgcg cgatcctcgc cgaccagctc gacacgctcg agaagcgcgc
     3121 ggccggcgcc ccgatcgacc tctcgctgct gaccgacggc ctcgaggccg aacgcgaaca
     3181 aggcatcacg atcgacgtcg cctaccgcta cttcgcgacc cggcagcgca agttcatcat
     3241 cgccgacgcg ccgggacacg agcagtacac gcgcaacatg gtgacggccg cggccggcag
     3301 cgatgcggcg gtcgtgctgg tcgacatcac gaagctcgac acgcatgcgc tgcccgtgaa
     3361 gctgctggct cagacgcggc gccacgcgct gctcgcgcaa ctgctgcgcg ttcccagcat
     3421 cgtgttcgcg atcaacaagc tcgacgcggt cgacgacccg gcgcaggcgt acgaggcggt
     3481 gcgtgccgcg ctgctcgatt tcgccgccga ggcgaagatg gaagtcgccg gcatcatccc
     3541 ggtctcggcg ctgcgcggcg acaacgtcac gcagccgccg gcggcagcga acgcgaccct
     3601 gggggcctgg gccagctggt accagggccc gtcgctgctg cagttgctcg agagcctgcc
     3661 gaccgcgcag gagcgtacgg tcggcgatct gctgatcccc gtgcagtacg tcgcgcgcga
     3721 cagcgacggc gtgggcgaca agccgcgcac gctgtggggc cggatcgcgc atggccgcgt
     3781 ccaggccggc gacgaagtgc agctgttccc gagccggcag aaggccgtcg tcgccgaggt
     3841 gcgcgtggcc ggtgaactgg tcgatgcagc ggaagcaggc cagtccgcgg gcctgagcct
     3901 cgaccgccag ctcgacgtct cgcgcggcga ctggatcgcc acacccgaca cggtgcatga
     3961 gacgaaccgc ttctcggcga ccctcgcctg gctcgacacc gagccggccg tgatcggccg
     4021 caagtactcg gtgcgccacg gcaaccgctg ggtgctggcg cgcatcaccg cgatcgagag
     4081 ccggctcgac atccactcgc tgcaggcgac cgacgcgcac gagctggccg tcaacgagat
     4141 cggccatgtc gtgatcgaga cccagcagcc gctgccggtc gaggccta
//
LOCUS       DASRDG010000426         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_425228, whole genome shotgun sequence.
ACCESSION   DASRDG010000426 DASRDG010000000
VERSION     DASRDG010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425228"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="VFA35_07075"
     CDS             complement(<1..1821)
                     /locus_tag="VFA35_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZT55970.1"
                     /translation="MAGLFQNQRNRWLPIAAAAACSAALAALLFSGVRLAARLQTAST
                     ALQLASSLSSQPKFLRSELTLIQRGLETRTYVGNSLRALEASRAASNQAYGRLALAMR
                     QAGFASESDAAGLYERARADWRPLDAGLSRLATANRSGLYADSASGSTLTAAGAALKR
                     TVDELLAAQTHATTALESGLGALAAQLRETVVHDGRSLRGLLLGGAGLASLLLAAMLY
                     FALRAGRAAEAAAEAERQVGNLLGTVREGLFLITRDGRVGRAHSDSLPALLHVPAPAD
                     QSFEELLRPLVDEKTLLAASKYLGLLHRDKVNEELIESVNPLGQIEVNLPRTQGPAER
                     RFLSFSFRRARGAHDLVFGSVADVTERVLLQRELEQLRTDQDSQAALLLQLLGAGPVQ
                     LHSFLSNVDVGVRRSNALLRSPGTSPAELQHKLAGVFREMHALKGEAAALGLQSFVQR
                     THQIEDLLDGLRPRTDLGGDDFVPAVVKLDELVDHMQLVASMQDQLAHTRAAPPPVAE
                     LPPERHGDTAVLRPQEAPAVPADAPPPSLELSLQRLAAEITASGERSVRIVCEGLDRV
                     PEQYQATVRDLTIQLVRNAVVHGIEPRSARAAAGKPPVGTV"
     gene            complement(1844..>2591)
                     /locus_tag="VFA35_07080"
     CDS             complement(1844..>2591)
                     /locus_tag="VFA35_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZT55971.1"
                     /translation="TVKSMQADLAAERPRWYALQARTAIAAGDADSARKMLAQLQATG
                     TDRAKLTELQQAMETQQADARLVSLARRVHAAINAGALTEPAADSAQALVQQMQQLNR
                     NHPLTASAQQELQAALADRARAQQEAAAARARPETSAPAGAAAAPPPPLAAATQAGDD
                     YISAKPAVALDVVYPQRALDIRQQGYVIVEFTLNADGRAADPHVVESSPPAVFDAAAL
                     QAVRHGRFDTRALGAVAKPHLARLRISFKP"
BASE COUNT          346 a          905 c          964 g          376 t
ORIGIN      
        1 caccgtgccc accggcggct tgccggcggc ggcgcgcgcc gatctcggct cgatgccgtg
       61 cacgacggca ttgcgcacca gctggatggt gaggtcacgc accgtggcct ggtattgctc
      121 cgggacgcga tcgagtcctt cgcacacgat ccgcacggaa cgctcgccgg aggcggtaat
      181 ctcagcggcg agtcgctgca gcgagagttc caggctgggc ggtggcgcgt ccgcgggcac
      241 ggcgggcgct tcctgcggcc gcagcacggc ggtatcgccg tgccgttcgg gcggcagctc
      301 ggcgaccggc gggggcgctg cgcgcgtgtg cgcgagctgg tcctgcatgc tggccacgag
      361 ttgcatgtgg tcgacgagct cgtcgagctt cacgaccgcg gggacgaaat cgtcgccacc
      421 gagatcggtg cgggggcgca ggccatccag caggtcctcg atctggtgcg tgcgctggac
      481 gaaggattgc aggccgagtg ctgccgcctc gcccttgagc gcgtgcattt cgcggaacac
      541 gcccgcgagc ttgtgctgca gttccgccgg cgaggtgccc gggctgcgca gcagcgcatt
      601 ggagcggcgc acgccgacgt cgacgttgct gaggaacgag tgcagctgca ccggtccagc
      661 gcccagcagc tgcagcagca gcgccgcctg cgagtcctgg tcggtgcgca gctgctcgag
      721 ctcgcgctgc agcagcacgc gctcggtgac gtcggcaacc gagccgaaca ccaggtcgtg
      781 cgcgccgcgc gcgcggcgga acgagaacga caggaagcgc cgctccgccg gaccctgggt
      841 tcgcggcagg ttgacctcga tctggccgag cggattgacg gactcgatga gttcctcgtt
      901 gaccttgtcc cggtgcagca ggccgaggta cttgctggct gcgagcagcg tcttttcgtc
      961 cacgagcggg cgcagcagct cctcgaagct ctggtcggcc ggcgccggca cgtgcagcag
     1021 cgctgggagt gagtcggagt gggccctgcc cacgcgcccg tcgcgcgtga tcaggaacag
     1081 gccttcgcgc accgtgccga ggaggttccc gacctggcgt tcggcttcgg cggccgcctc
     1141 ggcggcgcgc ccggcgcgca aggcgaaata gagcatcgcc gcgagcagca ggctcgccag
     1201 cccggcacca ccgagcagca ggccgcgcag cgagcggccg tcgtgcacga ccgtctcgcg
     1261 cagttgcgcc gccagcgcgc cgagcccgga ttccagcgca gtcgtggcgt gcgtctgcgc
     1321 cgccagcaac tcgtcgaccg tgcgcttgag cgccgcgccg gcggccgtca gcgtcgaacc
     1381 gctggcagag tcggcgtaca ggcccgagcg gttggcggtc gcgagccgtg acagccccgc
     1441 atccagcggt cgccagtcgg cgcgcgcgcg ctcatagagg ccggccgcat cgctctcgga
     1501 agcgaacccc gcctggcgca tggcgagcgc caggcgcccg taggcctggt tgctggccgc
     1561 gcggctcgcc tcgagcgcac gcagcgaatt gccgacgtag gtgcgcgtct cgagtccgcg
     1621 ctggatcagg gtcagctccg agcgcaggaa cttcggttgc gagctcagcg aagatgccag
     1681 ctgcagcgcg gtcgacgcgg tctgcagccg cgcggccagt cgcacgccgg aaaacagcaa
     1741 cgccgccagc gcggcgctgc acgccgccgc cgcggcgatc ggcagccatc ggttgcgctg
     1801 gttctggaac aggcctgcca tgtgcgactc cgcggaggtg agactacggc ttgaagctga
     1861 tgcgcaggcg cgccaggtgc ggcttcgcca ccgcgccgag cgcgcgcgtg tcgaagcgcc
     1921 cgtgccgcac ggcctgcagc gcggccgcgt cgaacacggc cggcgggctc gattccacca
     1981 cgtgcgggtc ggcggcgcgg ccgtcggcgt tgagcgtgaa ttcgacgatg acatagccct
     2041 gctggcgtat atcgagggcc cgctgcggat agaccacgtc cagcgccacc gcgggcttgg
     2101 ccgagatgta gtcgtcgccg gcttgggttg cggcggcaag cggtggcggc ggcgcggccg
     2161 cggcacccgc gggcgcgctc gtctccggac gcgcgcgcgc ggcagcggcc tcctgctggg
     2221 ctcgggcccg atcggccagc gccgcctgca attcctgctg ggcgctcgcc gtcaacggat
     2281 gattgcggtt gagttgctgc atctgctgca ccagcgcctg ggcactgtcg gccgcaggct
     2341 cggtgagcgc gccggcgttg atcgcggcgt gcacgcgccg cgcgagcgac accaggcgcg
     2401 catcggcctg ctgggtttcc atcgcctgtt gcagctcggt gagcttcgcg cgatcagtcc
     2461 cggtcgcttg cagctgggcg agcatcttcc tggcgctatc ggcatcgccg gcggcgatcg
     2521 cagtacgcgc ctgcagcgcg taccagcgcg ggcgctccgc agccagatcg gcctgcatcg
     2581 acttcacggt g
//
LOCUS       DASRDG010000427         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1576463, whole genome shotgun sequence.
ACCESSION   DASRDG010000427 DASRDG010000000
VERSION     DASRDG010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576463"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VFA35_07085"
     CDS             complement(<1..814)
                     /locus_tag="VFA35_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT55972.1"
                     /translation="MLVFVLRRLAQAVIVMLTVAFIAFMLFQYVGDPVTNILGQDATP
                     QQREQLRADLGLDKPFPVQFARFVGKAVQGEFGLSLRQGRKVSSLIAERLPATLELAL
                     CAAFLALALGIPMGVYAALRRGSFLSQALMTVSLIGVSLPVFLIGILLILVFAVTLNW
                     LPSFGRGEVTMLGHWSTGFLTVDGWQHLILPSITLAVFQLALIMRLVRSEMLEVLRAD
                     YIKFARARGLPNRVVYFGHALKNTLVPVLTITGLQLGSLIAFAIITEQVFQWP"
     gene            complement(999..2051)
                     /locus_tag="VFA35_07090"
     CDS             complement(999..2051)
                     /locus_tag="VFA35_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZT55973.1"
                     /translation="MKKSLLALAVLGAFAGVASAQSSVTLYGTVDLAARYVKNDGQSR
                     RLSMANSGLNSSQLVFSGKEDLGGGLWAGFNLNTGVAADTGNPAGGSQFWNRRSTVSL
                     GGGFGEIRLGRDYSVTFWNQTIFDAFGTNGVGDSTHVSQFSAMQPSFVRTANMISYLL
                     PSNIGGIYGQFQAAASEGANSNAGRYIGGRVGFAAGPFDIAGAFAEQRYDLLGKPQQK
                     IFNIGASYDLSVAKLMGYYERDTLSYPGFDKKENRFALSAVIPVGQGEIHVGGERSKL
                     KNAVANTSNTITQYSAGYVYNLSKRTALYTTVSRLKNGSLSQAQVDGGGGSTVGPLPT
                     LGGKSTGADFGIRHFF"
     gene            2349..2966
                     /gene="coq7"
                     /locus_tag="VFA35_07095"
     CDS             2349..2966
                     /gene="coq7"
                     /locus_tag="VFA35_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HZT55974.1"
                     /translation="MDSLITAADATLRTLSGASHAARACPRPEAPGAVLDDKQRRLSG
                     ALMRVNHVGEVCAQALYTAQALGTRNATLQARFEHAAREETDHLAWTRQRLRELGARP
                     SLLNPVWYAGSFALGLVAARAGDAVSLGFVVETERQVEQHLQAHLQRLPATDTASQAI
                     VQQMKEDEARHGAAAQAAGAAALPAPVRWAMRLAARVMTTTAHRI"
     gene            complement(2977..3399)
                     /locus_tag="VFA35_07100"
     CDS             complement(2977..3399)
                     /locus_tag="VFA35_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZT55975.1"
                     /translation="MECTINWMPANGMAFSAETGSGHLLTMDGAPDGGGRNLAPRPME
                     TLLAGAGGCTAYDVVLILQRGRHDVRGCQLVMTSERAPTDPKVFTKIHLHFVVTGPAL
                     TDAAVERAVKMSHDKYCSATIMLAKTAEITTSFEIKAG"
BASE COUNT          640 a         1147 c         1150 g          532 t
ORIGIN      
        1 cgggccactg gaacacctgc tcggtgatga tggcgaaggc gatgagcgag ccgagctgca
       61 agccggtgat cgtgaggacc ggcaccaggg tgttcttcag cgcgtggccg aagtacacga
      121 cccggttcgg cagcccgcgc gcgcgtgcga acttgatgta gtcggcgcgc agcacctcga
      181 gcatctcgga gcgcaccagc cgcatgatga gcgcgagctg gaacaccgcc agcgtgatcg
      241 acggcaggat caggtgctgc cagccgtcga ccgtcagaaa gccggtgctc cagtggccaa
      301 gcatcgtcac ctcgccccga ccgaagctcg gcagccagtt cagcgtgacc gcgaacacca
      361 ggatcagcag gatgccgatc aggaacaccg gcagcgacac gccgatcagc gacacggtca
      421 tcagcgcctg cgacaggaag ctgccgcgcc gcagcgccgc gtagacgccc atcgggatgc
      481 cgagcgcgag cgcaaggaac gcggcgcaga gcgcgagttc gagcgtcgcc ggcaggcgct
      541 cggcgatcag cgacgacacc ttgcggccct ggcgcaggct cagcccgaac tcgccctgca
      601 ccgccttgcc gacgaagcgc gcgaactgca ccgggaacgg cttgtcgagg ccgaggtcgg
      661 cgcgcaactg ctcgcgctgc tgcggcgtcg cgtcctggcc gaggatgttc gtcaccgggt
      721 cgccgacgta ctggaacagc atgaacgcga tgaaggcgac cgtgagcatc acgatcacgg
      781 cttgggcgag gcggcgaagg acaaagacga gcatcaggga cgggacgttt cagtttgcga
      841 tcacagcaaa acgcgagcca accgtcaaag gggaatgccc cgatgcccga ccgccgccaa
      901 ggcactcgat catgccctgg gcacgaagcg gacaacgaaa aagccgcccg aaggcggctt
      961 ttggtttctg gcgaaggctt gcgcgagcaa gcgatcgatc agaagaagtg acggatgccg
     1021 aaatcggcgc cggtcgactt gccgcccagg gtcggcagcg ggccaaccgt gctgccgccg
     1081 ccgccgtcga cctgcgcttg cgacagcgag ccgttcttca gacgcgaaac ggtcgtgtac
     1141 agcgcggtgc gcttcgacag gttgtacacg tagccagccg agtactgggt gatcgtgttc
     1201 gaggtgttcg ccacagcgtt cttcagcttg ctgcgctcgc caccgacgtg gatctcgcct
     1261 tggccgacag ggatcaccgc gctcagcgcg aagcggtttt ccttcttgtc gaagccaggg
     1321 tagctcaggg tgtcacgctc gtagtaaccc atcagcttgg caacgctcag atcgtacgac
     1381 gcgccgatgt tgaagatctt ctgctgcggc ttgcccagca ggtcatagcg ctgctcggcg
     1441 aacgcgccgg cgatgtcgaa cggacccgcg gcgaagccga cgcggccacc gatgtagcgg
     1501 ccggcgttcg agttcgcgcc ttcgctggcg gcggcttgga actgaccgta gatgccaccg
     1561 atgttggacg gcagcaagta cgagatcatg ttcgcggtgc gcacgaacga aggctgcatc
     1621 gccgagaact gcgacacgtg ggtcgagtcg ccgacgccgt tggtgccgaa cgcgtcaaaa
     1681 atggtctggt tccagaaggt caccgagtag tcgcgaccga ggcggatttc accgaagccg
     1741 ccacccaagc tcaccgtcga gcggcgattc cagaactgcg aaccaccagc ggggttgccg
     1801 gtatcggcgg cgacaccggt gttcaggttg aagccggccc acagaccgcc gcccagatct
     1861 tccttgccgg agaacaccag ctggctgctg ttgaggccgc tgttggccat cgacagacgg
     1921 cgcgattgac cgtcgttctt cacatagcga gcggccaggt cgacggtgcc gtacagcgtg
     1981 acagacgatt gagccgatgc cacaccagcg aaagcgccca gaactgcgag agcgagcaga
     2041 gattttttca ttgcaactat ctcctaggtt gaacaagagg tcccgatcaa gaaatcaccc
     2101 tcgtcgggca gatcaggcac aaccccctca gcggaagttg tgcgtattgc accagagggg
     2161 ccgggtgctg gaaagccttg cgggccaaaa aaggacttta tgttgtctga gcacaacgag
     2221 ccagcgaggg ccgccgatgc atcgaatcga cgccccaaca tgcgcatgtc agcgcccgca
     2281 aacctcacgg gtcaagcgct tgcgttattc tggtgccgtc ccgtatcacc gcccgcacct
     2341 tgcctccgat cgactccctg atcactgccg ccgatgccac actgcgtacc ctgtcagggg
     2401 ccagccatgc ggcacgcgcc tgcccccggc cggaggcgcc cggcgccgtg ctcgacgaca
     2461 agcaacgtcg cctgtcgggc gcgctgatgc gcgtgaacca cgtcggcgag gtgtgcgcgc
     2521 aggcgctcta caccgcgcag gcgctgggca cgcgcaacgc aacgctgcag gcacgcttcg
     2581 agcacgccgc gcgcgaagag accgatcact tggcctggac ccggcaacgc ctgcgcgaac
     2641 tgggcgcacg cccgagcctg ctgaacccgg tctggtacgc cggctcgttc gccctcgggt
     2701 tggtcgcggc gcgcgccggg gatgcggtga gcctgggctt cgtggtcgag accgagcgcc
     2761 aggtcgagca gcacctgcag gcccatctgc agcgcctgcc ggccaccgac acggcgtcgc
     2821 aggccatcgt ccagcagatg aaggaagacg aggcccgcca cggcgcggcg gcgcaagcag
     2881 ccggcgccgc cgccctgccc gcgccggtgc gctgggcgat gcggctggcg gcgcgggtca
     2941 tgacgacgac cgcacaccgc atttgagaag cgtcggtcag ccggctttga tctcgaagct
     3001 ggtcgtgatc tcggccgtct tggccagcat gatggtggcc gagcagtact tgtcgtgcga
     3061 catcttcacc gcgcgttcga ccgccgcgtc ggtcagcgcc ggcccggtga cgacgaagtg
     3121 caggtggatc ttcgtgaaca ccttcgggtc ggtcggcgcg cgttccgagg tcatgaccaa
     3181 ctgacagcca cgcacgtcgt gccggccgcg ttgcaggatc agcaccacgt cgtaggcggt
     3241 gcaaccgccg gcgccggcga gcagcgtctc catcggacgc ggcgccaggt tgcggccgcc
     3301 gccatcgggc gcgccgtcca tcgtcagcag gtggccgctg ccggtctcgg cagaaaaggc
     3361 catgccgttc gcgggcatcc agttgatcgt gcattccatc gtctgatcct cgattccccg
     3421 caccgattgc gggcatctgc gcgtcgattg tgcacatccg cacggtccc
//
LOCUS       DASRDG010000428         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_296128, whole genome shotgun sequence.
ACCESSION   DASRDG010000428 DASRDG010000000
VERSION     DASRDG010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296128"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /gene="gyrB"
                     /locus_tag="VFA35_07105"
     CDS             <1..366
                     /gene="gyrB"
                     /locus_tag="VFA35_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827604.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HZT55976.1"
                     /translation="EALSGAGLTFRGLVAEGAVVKRGEGEKQKEMRVTDFREAMAWLI
                     QQAEGAVARQRYKGLGEMNPAQLWETTMDPAVRRLLRVQIDDAIEADRVFTMLMGDEV
                     EPRRNFIENNALRAANIDV"
     gene            complement(388..855)
                     /locus_tag="VFA35_07110"
     CDS             complement(388..855)
                     /locus_tag="VFA35_07110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT55977.1"
                     /translation="MTTIRLATPADAKAIAALSWREVEQHWPWHWNPPSALSAMLDRD
                     ANVALALGADGQLVGFGVMEYHDDTAHLSLLVVNATARRRGIGTMLLRWLERVAAVAA
                     IARIEVEERRSDTAALAFYASRGYVRSGVSGYYVRLCKALAQGGGAAARERSP"
     gene            995..1453
                     /locus_tag="VFA35_07115"
     CDS             995..1453
                     /locus_tag="VFA35_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZT55978.1"
                     /translation="MADIVHRVGIKAPASKVYAALSSIDGLAAWWTTATTGSSKVGEV
                     VAFRFHTETGDEIGGFDMEVLELVPDQKVRWRVKAGPEEWVGTDIEFLLSRQDDYTIV
                     MFGHRRWREEVEFMAHCSTKWATFLLSLRDLLETGKGKPAPHDLRIGNWH"
     gene            1449..3002
                     /gene="malQ"
                     /locus_tag="VFA35_07120"
     CDS             1449..3002
                     /gene="malQ"
                     /locus_tag="VFA35_07120"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZT55979.1"
                     /translation="MRRGDGPANAQSGPMNLNQRSAGVLLHITSLPGPHGIGDLGPEA
                     YHFVEWLASAGQRVWQILPTTPVGPGDSPYQSVSAFAGSPLMVALEPLVAKGWLAAPE
                     LPEEGFGEARVDYARVTPWRIAQLRASSENFFRRAGAEDHAAFEAWCAAQAHWLDDYV
                     LFMALETTHAGAAWWHWPQPLRAREPAALAKARRELAAEIGFWQFVQWCFDTQWAALK
                     AHANARGVFIMGDLPIFVAHHSADCWARPDLYELDAEFQPSVVAGVPPDAFSADGQRW
                     GNPLYRWDRMAAEDYAWWVARVRRALSQADAFRIDHFRGFAAHWEVPATSPTAIDGRW
                     VPGPGQRVFDALARALGELPIVAEDLGVITPDVTALRDACGFPGMRILQFAFGGDAGN
                     AYLPHHHTPASVVYTGTHDNDTSRGWWAAIAERERAFAGAYLAAEAQDVHWAMIRAAL
                     TSVANTAIVPMQDVLGLDGSHRMNLPGTPTGNWSWRFDWRMVGPEPGRVLGMMTAASG
                     RGPFALLRD"
BASE COUNT          453 a         1052 c         1070 g          440 t
ORIGIN      
        1 gaggccttga gcggcgccgg cctgaccttc cgcggcctgg tcgccgaagg cgcggtcgtc
       61 aagcgcggcg agggcgagaa gcagaaggaa atgcgcgtaa ccgacttccg cgaagcgatg
      121 gcctggctga tccagcaggc cgaaggcgcg gtcgcgcgcc agcgctacaa aggcctgggc
      181 gagatgaacc ccgcgcagct ctgggaaacg acgatggacc cggcagtgcg ccgcctgctg
      241 cgcgtgcaga tcgacgacgc gatcgaagcc gaccgcgtct tcacgatgct gatgggcgac
      301 gaggtcgaac cgcggcgcaa tttcatcgag aacaacgcgc taagggcggc gaacatcgac
      361 gtgtgaggcg cccggccttc gaccggttca gggcgagcgc tcccgtgccg ccgcgccgcc
      421 accctgcgcc agtgccttgc acaagcgcac gtagtagccg ctgaccccgc tgcgcacgta
      481 gccgcggctc gcgtagaacg ccagcgcggc ggtgtcgctg cggcgctcct cgacctcgat
      541 ccgtgcgatg gccgcgacgg cggccacgcg ttcaagccag cgcagcagca tcgtgccgat
      601 gccgcggcga cgcgcggtcg cattcaccac cagcaggctc aggtgcgccg tgtcgtcgtg
      661 gtactccatg acgccgaagc cgaccaactg cccgtccgcg cccagcgcca aggcgacgtt
      721 ggcatcgcgg tcgagcatcg cgctgagcgc gctcggcggg ttccagtgcc acggccaatg
      781 ctgctccacc tcgcgccacg acagcgcggc gattgccttc gcgtcggccg gcgtggcaag
      841 acggatggtc gtcatcacgg gacccatcgt acacaggccg gcgcacaggg cggcgccgcc
      901 ggatcgccgc ggattcgcga cgatcttgtt actcccgctc gcgtgctcgc tcgcatagat
      961 ttgctcgcat caacctcagc aattggagat cgacatggca gatatcgttc acagggtcgg
     1021 gatcaaggcg cccgcttcga aggtctacgc cgcgctgtcg agcatcgacg ggctggcggc
     1081 ttggtggacc acggccacca ccggcagctc gaaggtcggc gaggtcgtcg cgtttcgctt
     1141 ccacaccgag accggcgacg agatcggcgg cttcgacatg gaggtgctcg agctggtgcc
     1201 cgaccagaag gtgcgctggc gcgtcaaggc cggcccggag gaatgggtgg gtaccgacat
     1261 cgagtttctc ctctctcggc aggacgacta caccatcgtg atgttcggcc accggcggtg
     1321 gcgcgaggag gtggagttca tggctcactg cagcacgaag tgggccacct tcctgttgag
     1381 cttgcgcgac ctgctcgaga ccggcaaggg caagccggca ccgcacgacc tgcgcatcgg
     1441 caactggcat tagacgaggc gacgggccgg caaatgcaca atccggcccc atgaacctga
     1501 accaacgcag tgccggcgtg ctgctgcaca tcacctcgct gccgggcccg cacggcatcg
     1561 gcgatctcgg gcccgaggcc tatcacttcg tcgagtggct cgcgtcggcg ggacagcgcg
     1621 tttggcagat cttgccgacg acgccggtcg ggccgggcga ttcgccgtac cagagcgtct
     1681 cggcctttgc cggcagcccg ctgatggtgg cgctggaacc gctggtcgcg aagggctggc
     1741 tggccgcgcc ggagttgccc gaagagggtt tcggcgaggc gcgggtcgac tacgcgcgcg
     1801 tcacgccgtg gcgcatcgcg cagctgcgcg cgtcgagcga gaacttcttc cgccgcgccg
     1861 gcgccgagga ccacgccgca ttcgaggcct ggtgcgccgc gcaggcgcac tggctcgacg
     1921 actacgtgct gttcatggcg ctcgagacga cgcacgccgg cgccgcgtgg tggcactggc
     1981 cgcagccgct gcgcgcgcgc gaaccggcgg cgctcgcgaa ggcgcggcgc gagctcgcgg
     2041 ccgagatcgg cttctggcag ttcgtgcagt ggtgcttcga cacgcagtgg gcggcgctca
     2101 aggcgcatgc gaacgcgcgc ggcgtgttca tcatgggcga cctgccgatc ttcgtcgcgc
     2161 accacagcgc cgactgctgg gcccggcccg acctgtacga gctcgatgcc gagttccagc
     2221 ccagcgtcgt cgccggcgtg ccgcccgacg cgttcagcgc cgacggccag cgctggggca
     2281 acccgctgta ccgctgggac cgcatggccg ccgaagacta cgcctggtgg gtcgcgcgcg
     2341 tgcgccgcgc gctgtcgcag gccgacgcgt tccggatcga tcacttccgc ggtttcgcgg
     2401 cccactggga ggtgccggcg accagcccga ccgcgatcga cggccgctgg gtgcccggcc
     2461 ccggccagcg cgtcttcgat gcgctggcgc gcgcgctcgg cgagttgccg atcgtcgccg
     2521 aagacctcgg cgtgatcacg cccgacgtga ccgcgctgcg cgacgcctgc ggctttcccg
     2581 gcatgcgcat cctgcagttc gcgttcggcg gcgatgcggg caatgcctac ctgccgcacc
     2641 accatacgcc ggcctcggtg gtctacaccg gcacgcacga caacgacacc tcgcgcggct
     2701 ggtgggcggc gatcgccgag cgcgagcggg cgttcgccgg cgcctatctc gcggccgagg
     2761 cgcaggacgt gcactgggcg atgatccgcg ccgcgctgac ctcggtcgcg aacaccgcga
     2821 tcgtgccgat gcaggacgtg ctcggcctcg acggcagcca ccgcatgaac ctgccgggca
     2881 cgccgaccgg caactggagc tggcgcttcg actggcgcat ggtcggcccc gagcccggcc
     2941 gggtgctcgg gatgatgacg gcggcgagcg ggcgcgggcc gttcgcgctg ttgcgtgatt
     3001 gactttcaaa ggagc
//
LOCUS       DASRDG010000429         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1325748, whole genome shotgun sequence.
ACCESSION   DASRDG010000429 DASRDG010000000
VERSION     DASRDG010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325748"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFA35_07125"
     CDS             complement(<1..482)
                     /locus_tag="VFA35_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZT55980.1"
                     /translation="MTAQRPLLGAALLATAMLCFSLSDAAAKQLAPQLSPTQVAWYRF
                     ALLALTVVPLALRTPALWRSRRPLLQSVRAAALVGSALLFLNGLRLMPIAEATAMVFA
                     SPLFVTLLATLLLRETMTPWRWLPVVCGFAGVLTVLRPGSFAFGGAEVFPMLSSLAWA
                     V"
     gene            complement(532..822)
                     /locus_tag="VFA35_07130"
     CDS             complement(532..822)
                     /locus_tag="VFA35_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT55981.1"
                     /translation="MELWRLRATNTHLYPGAALAFAGKEAASAALGPGDQVLVEFADQ
                     GVAQGIVVGGGVGDPAVVQFGAHLTAKGTTVQPARWVLVRSADGLRVKRRES"
     gene            complement(836..1489)
                     /locus_tag="VFA35_07135"
     CDS             complement(836..1489)
                     /locus_tag="VFA35_07135"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZT55982.1"
                     /translation="MNRKRTGTDLIAGDVHQKLRTKILTLEIKPGARLIEDEISTLLD
                     AGRTPVREALLRLQGEGLVSRDRGWIVQPTDIASFRSIFENRIAIEGFASRLAAERAT
                     TADLDELQRLIDEMDHSDKIPRSETNRLNQLFHKDIVRLSANPFFAEAHERTQFQYWN
                     LRLPVIFMKDQLAVSAEQHKAILKALKRRNGDEAERATREHIATTMQIVADALDDEH"
     gene            complement(1555..2346)
                     /locus_tag="VFA35_07140"
     CDS             complement(1555..2346)
                     /locus_tag="VFA35_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZT55983.1"
                     /translation="MTTTPTSPAVELIAVTKHRGAQTVLKNVSCQVPRGQVVAVLGPS
                     GAGKSTLLRCVNHLETIDGGRIFVEGQLIGYEERAGVLHELPDSAISRQRRRIGMVFQ
                     SFNLFRHLTVLDNVMAGPMQVLGQPRAQAEAQARGLLKRVGLADKANAFPNQLSGGQQ
                     QRVAIARALAMEPAVMLLDEPTSALDPELSQEVIEAIRQLAEDGITMLIATHEMALAR
                     DFADRVLFMVAGELVEDAPARRFFENPQNERSRQFVSRLGAHESA"
     gene            complement(2352..3203)
                     /locus_tag="VFA35_07145"
     CDS             complement(2352..3203)
                     /locus_tag="VFA35_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZT55984.1"
                     /translation="MNTTLTGAAPATRRVVHRRHWGAWLFGALVLIALGLLVRAAIAS
                     KVIDLGVFAEYVFSPNILIGAGHSVMLGTIALLLAVIVGLTIAMMRVSGNPILAGFAA
                     SYVYLFRGTPMLIQLIFWFNAFPIMFPVLHLESPFTGWVLVHLSMTQVVTPYMAALAG
                     LSLAEGAYMAEIVRGGFLAVDAGQRDAARAIGMTHWTLMRKVVIPQAARIVVPATGNQ
                     YIMLLKSTSLASAIGYLELLRISTDIYAANFRVVELLCVAGFWYLAMTAFATAIQTAL
                     EKLYPHR"
     gene            complement(3214..>3456)
                     /locus_tag="VFA35_07150"
     CDS             complement(3214..>3456)
                     /locus_tag="VFA35_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZT55985.1"
                     /translation="VYMSAQFPGRYKIAGGNYMLTPYITSWGFAKNRTGLRDAVQKAT
                     QSMLDDGTYRKILEKWGVGGAVLPAISVNLPASKRK"
BASE COUNT          553 a         1171 c         1204 g          528 t
ORIGIN      
        1 acggcccagg ccagcgacga cagcatcggg aacacctcgg ccccgccgaa cgcgaagctg
       61 cccggccgca gcacggtcag cacgccggcg aaaccgcaga ccaccggcag ccagcgccac
      121 ggcgtcatcg tttcgcgcag cagcagcgtc gcgagcagcg tgacgaagag cggcgacgcg
      181 aagaccatcg cggtcgcctc ggcgatcggc atcagccgca ggccattcag gaacagcagc
      241 gccgagccga ccagcgccgc tgcgcgcacc gactgcagca gcggccggcg cgagcgccac
      301 aacgcaggcg tgcgcagcgc cagcggcacg acggtcagtg ccagcagcgc gaagcggtac
      361 cacgcgacct gcgttggcga cagttgcggc gcgagttgct tggccgccgc atcggacagc
      421 gagaagcaca acatggcggt cgcgagcagc gcggcgccga gcagagggcg ttgggcggtc
      481 aagggcagac aaagaggtgt gggcagcgcg ctgggggcag gtcgatgcgg gtcaggactc
      541 gcgccgcttc acgcgcaggc cgtcggcgct gcgcaccagc acccatcgcg cgggttgcac
      601 ggtcgtgccc ttggccgtca ggtgcgcgcc gaactgcacc acggccgggt cgcccacgcc
      661 gccgccgacg acgatgcctt gcgccacccc ctggtccgcg aattcgacca gcacctggtc
      721 gccggggccg agcgccgcag acgcggcttc cttgccggcg aacgccagcg cggctccggg
      781 gtagaggtgc gtgttcgtgg cacgcaggcg ccagagttcc atgtcgatgg atggatcagt
      841 gctcgtcgtc gagcgcgtcg gcgacgatct gcatggtggt cgcgatgtgc tcgcgcgtcg
      901 cgcgctcggc ctcgtcgccg ttgcggcgtt tcagcgcctt caggatcgcc ttgtgctgct
      961 ccgccgagac tgcgagctgg tccttcatga agatcaccgg cagccgcagg ttccagtact
     1021 ggaactgggt gcgctcgtgc gcctcggcga agaacgggtt ggccgaaagc cgcacgatgt
     1081 ccttgtggaa cagctggttc aggcggttcg tctcggagcg cgggatcttg tcgctgtggt
     1141 ccatctcgtc gatcagccgc tgcaactcgt cgaggtcggc cgtggtcgcg cgctcggcgg
     1201 ccagccggct cgcaaagccc tcgatcgcga tgcggttctc gaagatcgag cggaacgacg
     1261 cgatgtcggt gggctgcacg atccagccgc ggtcgcggct caccaggccc tcgccctgca
     1321 ggcgcagcaa ggcctcgcgc accggcgttc ggccggcgtc gagcagggtc gagatttcgt
     1381 cctcgatcag acgcgcaccg ggcttgatct cgagcgtgag aatcttcgtt cgcagcttct
     1441 ggtgcacgtc gcccgcgatc agatcggtac cggttctctt tcgattcatg aaggcctcgc
     1501 cgaatggttg gctgccattc tgacgcgctg gtgcgagggt ggtcatcgcg agggtcaggc
     1561 cgattcgtgc gcgcccaggc gcgacacgaa ctgccggctg cgttcgttct gcgggttctc
     1621 gaagaagcgg cgcgccggcg cgtcctcgac cagttcgccc gcgaccatga acagcacgcg
     1681 gtcggcgaag tcacgcgcca gcgccatctc atgcgtggcg atcagcatcg tgatgccgtc
     1741 ctcggcgagc tggcggatgg cctcgatcac ctcttgcgac aactcggggt cgagcgcgct
     1801 ggtcggctcg tcgagcagca tgaccgcggg ttccatcgcc agcgcacgcg cgatcgcgac
     1861 gcgctgctgc tggcctcccg aaagctggtt cgggaacgcg ttcgccttgt ccgccagacc
     1921 gacccgcttc aacagcccgc gcgcctgcgc ctcggcctgc gcgcgtggct gacccaagac
     1981 ctgcatcggg ccggccatca cgttgtcgag caccgtcaag tggcggaaca ggttgaaact
     2041 ctggaacacc atgccgatgc ggcgacgctg gcggctgatc gcgctgtccg gcaactcgtg
     2101 cagcacgccg gcgcgctcct cgtagccgat caactggcct tcgacgaaga tgcgcccgcc
     2161 gtcgatggtc tcgaggtggt tcacgcagcg cagcagcgtc gacttgcccg cacccgaggg
     2221 gccgagcacg gcaacgacct ggccgcgcgg aacctggcaa ctcacgttct tcagcaccgt
     2281 ctgcgcgccg cggtgcttgg tgaccgcgat cagttcgacg gccgggctgg ttggcgttgt
     2341 cgtcatcgcg gtcaccggtg cgggtagagc ttctcgagcg cggtctggat cgcggtcgcg
     2401 aaggcggtca tggccagata ccagaagccg gcgacgcaga gcagttcgac gacgcggaag
     2461 ttcgccgcgt agatgtcggt gctgatgcgc agcaactcca ggtagccgat cgccgaagcg
     2521 agcgaggtgg acttcagcag catgatgtac tggttgccgg tggccggcac gacgatgcgc
     2581 gcggcctgcg ggatcacgac cttgcgcatc agcgtccagt gcgtcatgcc gatcgcgcgg
     2641 gcggcgtcgc gctggcccgc gtcgaccgcg agaaagccgc cgcgcacgat ctcggccatg
     2701 taggcgccct cggccaggct cagcccggcc agcgctgcca tgtagggcgt cacgacctgc
     2761 gtcatcgaca ggtgcacgag cacccagccg gtgaacgggc tctccaggtg cagcaccggg
     2821 aacatgatcg gaaacgcgtt gaaccagaag atcagctgga tcagcatcgg cgtgccgcgg
     2881 aacaggtaga cgtacgacgc cgcgaagccg gccaggatcg ggttgccgct gacgcgcatc
     2941 atcgcgatcg tcaggcccac gatcaccgcc agcagcaggg cgatcgtgcc cagcatcacc
     3001 gagtggccgg cgccgatcag gatgttgggg ctgaacacgt actcggcgaa cacgccgagg
     3061 tcgatgacct tggacgcgat cgccgcgcgc accagcaggc cgagggcgat caacaccagg
     3121 gcgccgaaga gccacgcgcc ccagtgcctg cggtgcacga cgcggcgcgt ggccggtgcg
     3181 gcgccggtga gggtggtgtt caccgccgac gccttacttg cgcttgctcg ccggcaggtt
     3241 caccgagatc gccggcagca ccgcgccgcc gacgccccac ttctcgagga tcttgcggta
     3301 cgtgccgtcg tcgagcatcg actgcgtcgc cttctggacc gcgtcgcgca ggccggtgcg
     3361 gttcttcgcg aagccccacg acgtgatgta gggcgtgagc atgtagttgc cgcccgcgat
     3421 cttgtagcgc cccgggaact gcgcgctcat gtagac
//
LOCUS       DASRDG010000430         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1097569, whole genome shotgun sequence.
ACCESSION   DASRDG010000430 DASRDG010000000
VERSION     DASRDG010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097569"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFA35_07155"
     CDS             complement(<1..644)
                     /locus_tag="VFA35_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZT55986.1"
                     /translation="MFDDQSILITGGTGSFGKAFLRTVLRDHNPRRLVVYSRDELKQY
                     EMQQEFDHPAMRFFIGDVRDAPRLARAMSGIDYVVHAAALKHVPVAEYNPMECIKTNV
                     NGAQNVIDAAIACGVKKVIALSTDKAANPINLYGASKLASDKLFSAANNMVGTNPTRF
                     SVVRYGNVIGSRGSVVPFFRGLLARGATELPITDPRMTRFWITLDQGVDFVIKC"
     gene            761..1894
                     /locus_tag="VFA35_07160"
     CDS             761..1894
                     /locus_tag="VFA35_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZT55987.1"
                     /translation="MTSFAISFVFSCVICAFIVRASRLQALPWDDDELTGAQKFHAQA
                     VPRVGGLAVVLGLVALTAWTVITGHGPARELCLILLCGLPALLAGLAEDLTKRVSAKF
                     RLAATMLSGLLAFFLLGAQLGSVSFPGLDWLMSFTAFSLLFTMVAAGGLANAVNIIDG
                     YNGLTAVVASVILMSLAYVAWQVGDNLIVSVALAGTGALVGFLLWNWPRGLIFLGDGG
                     AYFVGYLIAVLSIMLVARHRGVVSPWYPFLLCIYPVFETFFSIWRKRVVRGASPGVPD
                     GLHLHMLVFRRLVRWAVGRRDAASLTLRNSLTAPYLWLLSSIAALPATLLWRNPPALQ
                     AAVAVFCVLYVWLYLRLVRFRSPRWLVLRSKKGGALSRFRDGW"
     gene            2037..3833
                     /locus_tag="VFA35_07165"
     CDS             2037..3833
                     /locus_tag="VFA35_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HZT55988.1"
                     /translation="MTTTRIAAATADSAAPLSMMLAAGATMAIATPTLLAFNLPPSST
                     FLNQAVALIGWGVWLVMLTGALPRAGGPSPRGLVTLQAALAVLLAAALAAPLWAELPG
                     SLSLSSAALIGAAALAAQTAAGVSRTDLALPAFRAFCIGMVVAGALSSVVGVIQVFAP
                     NWPDGNWIARTYMEGRAVGNMRQPNHLSSLLLWAIVAVVWLGEARVVKRWGSALLALL
                     FILVVVLSASRTGALSMGLLLLWGALDKRLSRPARALLVLAPLIYGVFWYGTGVWADH
                     THHVFGGETRFSGKGDISSSRYAIWSNTLALIRMHPWAGVGFGEFNFAWTLTPFPDRP
                     VAFFDHTHNIALQFLVELGIPLGLLVLGLLLYAFAVAFRRALQPAADGAAPGAALLRP
                     AAFLMVLLILLHSLLEYPLWYAYYLLPAAFAFGLCLGGVPAARAEAVMRGMPVWRRPL
                     VVASLLLALGGAASLLDYLRVVYIFAPPEHSQVSLAQRIVDGRGSVFFAHHADYAAAT
                     TAGRPADVMPAFLRAPHYLLDARLLQAWATAFDEAGDTEHARYLAQRLREFRNEQSAE
                     FFAPCAAPSKAAKALPFQCLAPGREFRYEDFR"
     gene            3967..4158
                     /locus_tag="VFA35_07170"
                     /pseudo
     CDS             3967..4158
                     /locus_tag="VFA35_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
     gene            complement(4307..>4635)
                     /gene="moaC"
                     /locus_tag="VFA35_07175"
     CDS             complement(4307..>4635)
                     /gene="moaC"
                     /locus_tag="VFA35_07175"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZT55989.1"
                     /translation="KGDVIGVARIAAIQAAKRTAELIPLCHPLPITRVAVEFELDAAA
                     SSVRCTAQVETLGRTGVEMEALTAVQIGLLTIYDMCKAADRGMVMGEIRVLEKHGGKS
                     GDWVAR"
BASE COUNT          579 a         1619 c         1598 g          839 t
ORIGIN      
        1 aagcacttga tgacgaaatc gacgccctgg tcgagggtga tccagaagcg cgtcatccgc
       61 gggtcggtga tcggcagctc ggtcgcgccg cgcgcgagca ggccgcgaaa gaacggcacg
      121 accgagccgc gcgatccgat cacgttgccg tagcgcacca ccgaaaaccg cgtcgggttc
      181 gtaccgacca tgttgttggc ggccgagaac agcttgtcgg aggcgagctt ggaggcaccg
      241 tacaggttga tcgggttggc cgccttgtcg gtcgacagcg cgatgacctt cttgacgccg
      301 caggcgattg ccgcgtcgat gacgttctgt gcgccgttga cgttggtctt gatgcactcc
      361 atcgggttgt actcggccac cggcacatgc ttgagcgcgg cggcgtggac gacgtagtca
      421 atgcccgaca tcgcgcgcgc caggcgcggc gcgtcgcgca cgtcgccgat gaaaaagcgc
      481 atcgccgggt ggtcgaactc ctgctgcatc tcgtactgct tgagttcgtc gcgggagtag
      541 acgacgaggc ggcgcgggtt gtggtcgcgc agcacggtcc gcaggaaggc cttgccgaaa
      601 gacccggttc cgccggtgat cagaatgctt tggtcgtcga acatgcaggt cgcggttgac
      661 gcagtggagt ggcacgttac acgatttccc cggtggggcg acacgtcccg tgccgtgaag
      721 catcgtaagc tgcgcacttt gcggcagctg caccccgccc atgaccagct tcgcgatctc
      781 gttcgtcttc tcctgcgtca tttgtgcatt tatcgtccgg gcctcgcggt tgcaagccct
      841 gccctgggac gatgatgaac tgaccggggc ccagaaattc catgcacaag cggttccgcg
      901 cgtcggcggg ctggcggtcg tgcttgggct ggtagcgctc accgcatgga cggtcatcac
      961 gggccatggc ccggcccgcg aactctgcct gatcctgctc tgcggcctgc ctgccctcct
     1021 cgccggcctc gccgaagacc tcaccaaacg cgtcagcgcc aagttccgcc tcgccgcgac
     1081 gatgctcagc gggcttctcg cgttcttcct gctcggcgcg caactcggtt cggtgtcgtt
     1141 cccgggcctg gactggttga tgagcttcac cgcgttcagc ctgctgttca cgatggtcgc
     1201 ggcaggcggg ctggcgaacg cggtcaacat catcgacggc tacaacgggc tgacggcggt
     1261 ggtggcgagc gtgatcctga tgtcgctggc ctacgtcgcc tggcaggtcg gcgacaacct
     1321 gatcgtctcg gtggcattgg ccggcaccgg cgcgctcgtc ggctttctgc tgtggaactg
     1381 gccgcgcggc ctgatcttcc tgggcgacgg cggagcctac ttcgtcggct atctgatcgc
     1441 ggtgctgtcc atcatgttgg tcgcgcgcca ccgcggcgtg gtctcgccgt ggtacccgtt
     1501 cctgctgtgc atttacccgg tgttcgaaac cttcttctcg atctggcgca agcgcgtggt
     1561 gcgcggcgcg agccccggcg tgcctgatgg gctgcacctg catatgctcg tgttccgccg
     1621 cctcgtgcgc tgggcggtgg gccggcgcga cgccgcgagc ctgacgctgc gcaacagcct
     1681 gaccgcgccc tacctgtggc tgctgtcgag catcgccgcg ctgccggcca cgctgctgtg
     1741 gcgcaacccg cccgcgttgc aggcggcggt cgcggtgttc tgcgtgctct acgtgtggct
     1801 gtacctgcgc ctggtgcgct tccgttcgcc gcgctggctg gtgctgcgct cgaagaaggg
     1861 cggtgcgttg agccggtttc gcgacggctg gtgagcaccg cgcggcgggt accggctgcg
     1921 tggacaatgc cgcgcaggct gaccgcgacc gtctgtgccg ccatgcccgc tgccgggcgc
     1981 cccctgcaca tgatcgttcc cgggcgcgcc gcacggacga acgacaatgc gcgcccatga
     2041 caacaactcg catcgccgcc gcaaccgctg atagcgccgc cccgctgtcg atgatgctcg
     2101 cggcgggcgc caccatggcg atcgcgacgc cgacgctgct ggccttcaac ctcccgccgt
     2161 cgtcgacctt cttgaaccag gccgtcgcgt tgatcggctg gggcgtgtgg ctggtgatgc
     2221 tgaccggcgc gctgccccgc gcgggcgggc cctcgccgcg cggcctcgtg accttgcagg
     2281 cggcgctggc cgtgctgctg gccgccgccc ttgcggcgcc gctctgggcc gagctgcctg
     2341 ggtcgctgtc gctctcgtcg gcggcgctga tcggcgctgc cgcgctcgcg gcgcagaccg
     2401 cggccggcgt gtctcgcacc gacctggcgt tgcctgcgtt tcgcgcgttc tgcatcggca
     2461 tggtcgtcgc cggcgcgctc agcagcgtcg tcggcgtgat ccaggtcttc gcgccgaact
     2521 ggcccgacgg caattggatc gcccgcacct acatggaagg ccgcgcggtc ggcaacatgc
     2581 gccagccgaa ccacctgagc agcctgctgt tgtgggcgat cgtcgcggtg gtgtggctcg
     2641 gcgaggcgcg cgtcgtcaag cgctggggca gcgcgctgct cgcgctgctg ttcattctcg
     2701 tcgtcgtgct cagcgcctcg cgcaccggcg cgttgagcat gggcctgctg ctgctctggg
     2761 gcgcgctcga caagcgcctc tcgcgccccg cccgcgcgtt gctggtgctc gccccgctga
     2821 tctacggcgt gttctggtac ggcaccggcg tgtgggccga tcacacgcac catgtcttcg
     2881 gcggcgagac gcgcttcagc ggcaagggcg acatctcgag ttcgcgctac gccatctggt
     2941 cgaacacgct cgcgctgatc cgcatgcacc cgtgggccgg cgtgggcttc ggcgagttca
     3001 acttcgcgtg gacgctgacg ccgtttcccg atcggccggt ggcgttcttc gaccacacgc
     3061 acaacatcgc gctgcagttc ctggtcgaac tcggcatccc gctcgggctg ctggtgctgg
     3121 gcttgctgct gtacgcattc gccgtcgcgt tccgccgggc cctgcagccg gcggcggacg
     3181 gcgcagcgcc cggggcggcg ctactgcgcc ccgccgcgtt cttgatggtg ctgctgatcc
     3241 tgctgcacag cctgctggag tacccgctct ggtatgcgta ctacctgctg ccggcggcgt
     3301 tcgcgttcgg gctgtgcctg ggcggcgtgc cggcagcgcg cgccgaggcg gtgatgcggg
     3361 ggatgccggt ctggcgacga ccgctcgtcg tcgcgtcgtt gctgctggcg ctgggcggcg
     3421 cggcgtcgct gctcgattac ctgcgcgtcg tctacatctt cgcgccgccc gagcactcgc
     3481 aggtgtcgct cgcgcagcgc atcgtcgacg gtcgcggcag cgtgtttttc gcccaccatg
     3541 ccgactacgc cgccgcgacc accgctgggc gcccggccga cgtgatgccg gccttcctgc
     3601 gcgcgccgca ctacctgctc gacgcgcgcc tgctgcaggc ctgggccacc gcgttcgacg
     3661 aggccggcga caccgagcac gcgcgctacc tcgcgcagcg cctgcgcgag ttccgcaacg
     3721 agcagtcggc cgagttcttc gcgccctgcg ccgcgccgtc caaggccgcc aaggcgctgc
     3781 cgttccagtg cctcgcgccg ggccgtgagt tcaggtacga ggactttcga tgacgcggtc
     3841 gacgatgtgt attcgatcga acacaatcga cacgtgttcg cgattcgccg gctgcgcgag
     3901 ttctccggcc cgggttggcg cttgtactac gtcgtccgag gcggcacgtt gatcgtgatg
     3961 ctcggcggcg acgacggcga gctggcgcat gcgctgggcc acattgccaa ggcgcgtggc
     4021 atggcgcaaa tcgccaagga cgcggggatc ggccgggaga gtctttacaa ggcgttgcgc
     4081 cccgatgcca acccgcgctt cgacaccatc agccgcgtgt gcaaggccct gggcgtgagg
     4141 ctcgtggcgc agccgctggc agcgccggcg ccgacgctga agcaactcca gggcatcgtc
     4201 aagggcgcca atgccactgg ctatcgcgag cgcggcgcgc cccgctgatg cgcgcgaccg
     4261 ccagcccggc gcgaggcgtg cctggcgcag cgggctgaag gcggcgtcac cgcgcaaccc
     4321 aatctcccga cttccccccg tgcttctcca gcacgcggat ctcgcccatc accatgccgc
     4381 ggtcggcagc cttgcacatg tcgtagatcg tcagcaggcc gatctgcacc gcggtcagcg
     4441 cctccatctc gacgccggtg cgcccgagcg tctcgacctg cgcggtgcag cgcacgctgc
     4501 tcgcggccgc gtcgagctcg aactcgaccg cgacgcgcgt gatcggcagc gggtggcaca
     4561 gcgggatcag ctccgcggtg cgcttggcgg cctggatcgc cgcgatgcgc gcgacgccga
     4621 tcacatcgcc cttct
//
LOCUS       DASRDG010000431         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_425640, whole genome shotgun sequence.
ACCESSION   DASRDG010000431 DASRDG010000000
VERSION     DASRDG010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425640"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFA35_07180"
     CDS             complement(<1..463)
                     /locus_tag="VFA35_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HZT55990.1"
                     /translation="MSTSSSRTPADAPRWPGQLAAVDMGSNSFRLEIGQLVGGRYRRV
                     DYLKETVRLGAGLDADGMLTEEAGARGLACLARFAQRLQGFMPAQVRAVATQTLREAR
                     NRDAFLLGAQAALGFPIEVISGREEARLIYAGVARLQPSTLPRLVVDIGGRS"
     gene            563..1438
                     /locus_tag="VFA35_07185"
     CDS             563..1438
                     /locus_tag="VFA35_07185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZT55991.1"
                     /translation="MPTTAELDRPADAPDLSRRDFVRTAVGTGFAAAVLPVGAQTIKT
                     DSSGLTAGEVTIPVGDFKMPAYRAAPAGKRRLPVVLVVSEIFGVHEHIADVARRFAKL
                     GYLAIAPELFVRQGDAQSYGEIGKLLSEVVGKVPDAQVMGDLDAAVAWAGANGGDTSR
                     LGITGFCWGGRITWLYAVHSPSVKAAVAWYGQLIGPPTELKPTNPIDLVGKLNAPVLG
                     LYGAEDQGIPLAAVDQMKAALATGNAAAKASRFVVYPGAPHAFHADYRPSYQKDAAED
                     GWRRCVAWFKANGVA"
     gene            complement(1450..1884)
                     /locus_tag="VFA35_07190"
     CDS             complement(1450..1884)
                     /locus_tag="VFA35_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZT55992.1"
                     /translation="MFKRILVPTDGSEITAKAIDTAIELAKSVGAQLCTISVKEPFPY
                     SAISEMQPTPPQEFFEAQERIAARRVQAVVEQSREAGVPCQSHTVEALHPWEAIIDHA
                     KRLECDLLVMASHGRRGVSALLLGSETQKVLTHSKVPVLVVR"
     gene            complement(1950..>2606)
                     /locus_tag="VFA35_07195"
     CDS             complement(1950..>2606)
                     /locus_tag="VFA35_07195"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZT55993.1"
                     /translation="TNGRHPLPARADFAATLGHAGITPATQLVALDRQGGMYAARLWW
                     MLRWMGHDAVAVLDGGVAAWIASGGELVADAPTPIAAAPYPERAPLAATLDANALAAG
                     LGRVRLLDARAAERFRGEVEPLDAAAGHIPGALNRFFKDNLGSDGRFKPAAQLRAEFA
                     ALLGLRNAADTVHQCGSGVTACHNLLAMEHAGLPGSTLYPGSWSEWSADPARPVARSG
                     "
BASE COUNT          366 a          920 c          952 g          368 t
ORIGIN      
        1 tcgagcgccc gccgatgtcg accaccaggc gcggtagcgt cgagggctgc agccgcgcga
       61 caccggcgta gatcagccgc gcctcctcgc gccccgagat cacctcgatc ggaaagccga
      121 gcgcggcctg cgcgccgagc aggaaggcgt cgcggttgcg cgcctcgcgc agcgtctgcg
      181 tcgcgaccgc gcgcacctgc gcgggcatga agccctgcag ccgctgcgcg aaccgcgcga
      241 gacaggccag gccacgcgcg ccggcctctt cggtcagcat gccgtcggcg tcgaggccgg
      301 cgccgaggcg gacggtttcc ttcaggtagt cgacgcggcg gtagcggccg ccgaccaact
      361 gcccgatctc gagccggaag ctgttcgagc ccatgtcgac agcggccaac tggccgggcc
      421 agcgcggggc gtcggccggc gtgcgggagg aagaggtgct catgccccca ttgtcagcgc
      481 aaagcgtggg gtttttcacg gcccgcacct ggtccgcggt ctgtttctag aatgccgcct
      541 ccatgtccac ccggagccgc gcatgccgac gaccgccgaa ctcgatcgcc ccgccgacgc
      601 ccccgacctg agccggcgcg acttcgtgcg caccgccgtc ggcaccggct tcgccgctgc
      661 ggtgctgccg gtgggcgcgc agaccatcaa gaccgacagc agcggcctga ccgccggcga
      721 ggtcacgatc ccggtcggcg acttcaagat gccggcgtac cgcgcggcgc cggccggcaa
      781 gcgccgtctg ccggtcgtgc tcgtggtctc ggagatcttc ggcgtgcacg agcacatcgc
      841 cgacgtggca cggcgcttcg cgaagcttgg ctacctcgcg atcgcgcccg agctgttcgt
      901 gcgccagggc gatgcgcagt cgtatggcga gatcggcaag ctgctgagcg aggtcgtcgg
      961 caaggttccc gacgcgcagg tgatgggcga tctggacgcg gccgtcgcat gggccggggc
     1021 caacggcggc gacacgagcc ggctcggcat caccggcttt tgctggggcg ggcgcatcac
     1081 ctggctgtac gccgtgcaca gcccgtcggt gaaggcggcc gtggcgtggt acggccagtt
     1141 gatcggcccg ccgaccgagc tgaagccgac caacccgatc gatctggtgg gcaagctgaa
     1201 cgcgccggtg ctcggcctgt acggcgccga agaccagggc attccgctcg ccgcggtcga
     1261 ccagatgaag gccgcgctcg cgaccggcaa cgccgctgcc aaggcctcgc gattcgtcgt
     1321 ctaccccggc gcgccgcacg cgttccacgc cgactaccga ccgagctacc agaaggacgc
     1381 cgccgaggac ggctggcggc gctgtgtcgc ctggttcaag gcaaacgggg tggcgtgatc
     1441 gggacgggtt cagcgcacca cgagcaccgg caccttcgag tgcgtcagca ccttctgcgt
     1501 ctcgctgccg agcagcagcg ccgacacgcc gcgccggccg tgcgaggcca tcacgagcag
     1561 gtcgcactcg agacgcttgg cgtggtcgat gatcgcctcc cacgggtgca gcgcctcgac
     1621 ggtgtgcgac tggcagggta cgccggcctc ccgactttgc tcgaccacgg cttgcacccg
     1681 cctcgccgcg atgcgctcct gcgcctcgaa gaattcctgc ggcggggtcg gctgcatctc
     1741 cgagatcgcg ctgtacggaa aaggctcctt cacgctgatc gtgcagagct gggcgcccac
     1801 cgacttggcg agctcgatcg cggtgtcgat cgccttcgcg gtgatctcgg agccgtcggt
     1861 cggaacgagg atgcgtttga acatggtcgt ctccttcgct gtcggggcgg tgagacatca
     1921 tgcgccatcg aatgttcccg cgcgagcgtt cagccgctgc gcgccacggg ccgcgccggg
     1981 tcggccgacc actcgctcca cgagcccggg tagagggtcg agcctggcag gccggcatgt
     2041 tccatcgcga gcaggttgtg acaggcggtg acacccgagc cgcattggtg cacggtgtcc
     2101 gcggcgttgc gcaagcccag cagcgccgcg aactcggcgc gcagttgcgc ggcgggcttg
     2161 aagcggccgt cgctgccgag gttgtccttg aagaagcggt tcagcgcgcc ggggatgtgg
     2221 ccggcggccg cgtcgagcgg ctcgacctcg ccgcggaagc gctcggcagc gcgggcgtcg
     2281 agcaggcgca cgcggccgag cccggcggcg agcgcgttcg cgtcgagcgt ggcggcgagc
     2341 ggggcacgct cggggtacgg cgctgcggcg atcggggtcg gcgcgtccgc gacgagttcg
     2401 ccgcccgatg cgatccacgc ggccacgcca ccgtcgagca ccgcgaccgc gtcgtggccc
     2461 atccagcgca gcatccacca caggcgcgcg gcgtacatgc cgccctggcg gtcgagtgcg
     2521 acaagctgag tcgccggcgt gatgccggcg tggccgagcg tcgcggcgaa gtcggcgcgc
     2581 gcgggcagcg ggtggcggcc attcgt
//
LOCUS       DASRDG010000432         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_636779, whole genome shotgun sequence.
ACCESSION   DASRDG010000432 DASRDG010000000
VERSION     DASRDG010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636779"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFA35_07200"
     CDS             <1..224
                     /locus_tag="VFA35_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZT55994.1"
                     /translation="GVEIDLLHADRHLLMEVRDNGIGLPANGGTPGTGIRSMRTRARR
                     LGAQLIVDADEGITFIRLDIPISPPMHAG"
     gene            complement(324..566)
                     /locus_tag="VFA35_07205"
                     /pseudo
     CDS             complement(324..566)
                     /locus_tag="VFA35_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
     gene            750..4166
                     /locus_tag="VFA35_07210"
     CDS             750..4166
                     /locus_tag="VFA35_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZT55995.1"
                     /translation="MPSPFRFSGLPRKPTLLLAAVLATAVLLPFTQTAHAAAAQPAPA
                     QQVLPAGLEGAINDASAALARARDLDAGQRKQAEDLLRDALADDQAASDAVAARQSFL
                     DAAQKALPADAATAVPGSDAAQNFADWRARLPAGEDAAPFADLLESERNQLGAAQASL
                     HNLEEALQKQDQRPDALRDELAAARAAETAAAAPATDTPRTLAAAMRLRALAAERLKR
                     CRLAMLETEQRTYEARMRALFAQRTAARRGIDEHGQRVHWLENMVLDRYASRVSDLVT
                     RLGAERDKLDAEQAAPVLRETAAGNLALAQDLAATIKRNASLRDLQNGYASERNDTEQ
                     AAKNTKARLDLGGVSEDVGALLLAEQRKLRPLNSLKRELADVRNELAQGKLAGIDLRE
                     QQDRLENMDGAIAATLARAGQPPAITGPLHDGLERLLTTRADLYTRLIAAKTRQTTIA
                     EEAEQQLAGLIASTSTLNGMLDSSLFAIPSETPIGAAWFGELARALRGLVHPRQGAEV
                     AGFVDTVRANLALSAAALVMLLAAVYARRRVPTEFERLAAPLRRIRTDRFRYTMWALA
                     LTILAAAPVALFWTLLARLFLRTTNADPLGGTLGLATASIALPWFALAFLHWLNRDDG
                     LAHAHFRWLRARRVSLRRAVPWIALSVIVPIFIHNWLRETSGNETDATIGRALFIAGT
                     LGLAAIAWHLLAPGRVWAQRGAKLDDEPIRLRQFVRFGLSAAFLVLAALAAIGYFFTA
                     TLFTEHLLLSLMALLAVSVIHGLAVRWLTLGERRLALMRAEDRQLAELESVGPPVEGE
                     ALPEVEPEEMALADLGAQTRRVLRLVTVVLFAAVLLAIWSDIAPALSFLDGITVWKSS
                     STGADSKVIELNVTLRHVLESALLLVMTFIATRNLPGLLEIGVLRRLSIDAPTRYAIT
                     SVVRYLIVLGGVLVGLGLLGLHWANLQWLAAGLTVGLGFGLQEIFANFISGLIVLFER
                     PARVGDIITIGGIEGTVTRIRTRATTILDWDNKEVIVPNKAFITERLINWTLSDTTTR
                     VVIKVGVAYRNDARLARKLLLQVAEQHSLVLRDPAPLAWMTSFGDSSQDFELRVYVAE
                     INQRNLVRNELQMRIAEVFREHDIEIAYPTRDVWFRNALETNGAGRLEKP"
BASE COUNT          732 a         1581 c         1454 g          676 t
ORIGIN      
        1 cgggcgtcga aatcgatctg cttcatgcgg accgtcacct gctcatggag gtgcgcgaca
       61 acgggatcgg actgccggcg aatggcggca cgcctggcac cggcatccgc agcatgcgca
      121 cgcgcgcccg acggctgggc gcgcagctga tcgtcgacgc cgacgaaggc atcactttca
      181 ttcgactgga cattcccatt tcgccgccga tgcacgccgg ctagatcgtc accctgaaag
      241 caccggcgag acccgaacgg aacagcgaaa cgcacggcca cgacgcgcca gggcggcgcg
      301 tcgaagcgga tcgcggattg ttatttggcg ccgtagcgct tgcggaactt gtccacgcga
      361 ccgccggaat cgacgatctt gtgcttgccc gtgtagaacg ggtgcgaggc actggaaatt
      421 tcgatcttga cgaccggata ttcctggccg tcttcccact tgatcgtgtc cttgctcgtc
      481 atcgtcgaac gcgtgagaat cgcgaagttg gacgagacat cctggaagac cacaggcttg
      541 tattcgggat ggatgtcggc tttcatgatt tggctccgcg aagcggtatt gcgctgagaa
      601 aagaggcgaa ttctagcccg gattcgtgcc gccgcgcaac atcggggccg atcaagggcg
      661 tcgaacggcc atccgaccgg gctgcggcgt catttcggcg gcggcataat ggcttgcccg
      721 gccacttgcc gcccgaatcg caccaccaga tgcccagccc gttccgcttc tccggcttgc
      781 cgcgcaaacc cacgctgctg ctcgcagccg tcctcgcgac ggctgtgctg ctgccattca
      841 cgcaaaccgc gcacgcggcc gccgcgcaac cagcaccggc gcagcaggta ttgccagccg
      901 ggctcgaggg ggcgatcaac gatgcgagcg ccgcgctggc gcgtgcacgc gacctcgatg
      961 ccgggcagcg caagcaggcc gaggacctgc tgcgcgacgc actggccgac gaccaggcgg
     1021 ccagcgacgc ggtcgccgcc cgccagagtt ttctcgacgc ggcgcagaag gcgcttccgg
     1081 ccgatgccgc gactgcggtg ccgggcagcg acgcggccca gaatttcgcc gactggcgcg
     1141 cacgcctgcc ggccggcgaa gacgcagcgc cgtttgccga tctgctcgaa agcgagcgca
     1201 accagctcgg cgcggcccag gcttcgctgc acaatctcga agaggcgctg cagaaacagg
     1261 accagcgccc ggatgcgttg cgcgacgaac tcgcagccgc gcgcgcggcc gaaaccgccg
     1321 ccgcagcgcc ggcgaccgat acgccacgca cgctcgccgc ggcgatgcgc ctgcgcgcac
     1381 tcgctgcgga gcgcctcaag cgctgccgcc tcgccatgct ggaaaccgag cagcgcacct
     1441 acgaggcgcg catgcgcgcg ttgttcgccc aacgcacggc ggcccggcgc ggcatcgacg
     1501 agcacggcca gcgcgtgcat tggctggaga acatggtgct cgaccgctac gcgagccggg
     1561 tcagcgacct cgtcacccgg ctcggcgcgg agcgcgacaa gctcgacgcc gaacaggccg
     1621 caccggtgct gcgcgaaacc gccgcgggca acctcgcgct ggcccaggat ctcgccgcca
     1681 cgatcaagcg caacgccagc ctgcgcgacc tgcagaacgg atacgcgagc gagcgcaacg
     1741 acaccgaaca ggcagcgaag aacaccaagg cgcggctcga tctcggcggg gtcagcgagg
     1801 acgtcggcgc gctgctgctc gccgagcagc gcaagctgcg gccgttgaat tcgctcaagc
     1861 gcgagcttgc cgacgtgcgc aatgaactcg cgcaaggcaa gctggccggg atcgatctgc
     1921 gcgagcagca ggaccgcctg gaaaacatgg acggtgcgat tgccgccacg ctcgcgcgcg
     1981 ctggccagcc gcccgcgatc accggcccgc tgcatgatgg actcgaacgc ctgctgacca
     2041 cgcgcgccga cctgtacacg cggctgatcg cggcgaagac gcgacagacg acgatcgccg
     2101 aagaagccga acagcaactc gctggcctga tcgcgagcac cagcacgctc aacggcatgc
     2161 tcgactccag cctgttcgcg atcccgagcg aaacgccgat tggcgcggcc tggtttggcg
     2221 aactggcgcg cgcgctgcgt ggcctcgtcc atcccaggca gggcgcggag gtcgcgggct
     2281 tcgtcgacac cgtgcgcgcg aacctcgcgc tttcggcggc cgcgctggtc atgctcctgg
     2341 ccgcggtcta tgcgcgccgg cgcgtgccca cggaattcga acgcctcgcc gcgccgctgc
     2401 gccgcatccg caccgaccgc ttccgctaca cgatgtgggc gctcgcgctg acgattctcg
     2461 ccgccgcgcc cgtcgcgctg ttctggaccc tgctcgcgcg cctgttcctg cgcacgacga
     2521 acgccgaccc gctcggaggc acgctcggcc tcgccacggc gagcatcgcg ctgccctggt
     2581 tcgcgctcgc gttcctgcac tggctcaatc gcgacgacgg cctcgcccac gcgcatttcc
     2641 gctggctgcg cgcgcgccgc gtgtcgctgc gccgcgcggt gccgtggatc gcgctgtcgg
     2701 tgatcgtgcc gatcttcatc cacaactggc tgcgcgagac cagcggcaac gaaaccgacg
     2761 cgacgatcgg ccgcgcgctg ttcatcgccg gcacgctcgg tctggccgcg atcgcctggc
     2821 acctgctcgc gccgggacgc gtgtgggcgc agcgcggcgc caagctcgac gacgaaccga
     2881 tccgcctgcg ccagttcgtg cgcttcggcc tcagcgcggc cttcctcgtg ctcgccgcgc
     2941 tcgccgcgat cggctatttc ttcaccgcca cgctgttcac cgagcacctg ctgctgtcac
     3001 tgatggcgct gctcgcggtc agcgtgatcc acggcctcgc ggtgcgctgg ctcacgctcg
     3061 gcgagcgccg tctcgccctc atgcgcgcgg aggatcgtca gctcgcggaa ctcgaatcgg
     3121 tcggcccgcc ggtcgagggc gaggccctgc ccgaggtcga gcccgaggaa atggcgctgg
     3181 ccgatctcgg cgcacagacg cgccgcgtgc tgcgcctcgt caccgtagtg ctgttcgccg
     3241 ccgtgctgct cgcgatctgg tcggacatcg cgccggcgct gagcttcctc gatggcatca
     3301 cggtgtggaa atcaagttcc accggcgccg acagcaaggt gatcgaactc aacgtgaccc
     3361 tgcgccacgt gctcgaatcc gcgctgctgc tcgtgatgac cttcatcgcc acgcgcaacc
     3421 tgcccggcct gctcgaaatc ggcgtgctgc gtcggctaag catcgatgcg ccgacgcgct
     3481 acgcgatcac gagcgtcgtg cgctacctca tcgtgcttgg cggcgtgctc gtcggtttgg
     3541 gcctgctcgg cctgcactgg gcgaacctgc agtggctcgc cgccggcctc accgtcggcc
     3601 tcggtttcgg cctgcaggaa atcttcgcca acttcatctc gggactgatc gtgctgttcg
     3661 agcgcccggc gcgcgtcggc gacatcatca cgatcggcgg catcgaaggc acggtcacgc
     3721 gcatccgcac gcgcgcgacg acgatcctcg actgggacaa caaggaagtg atcgtgccga
     3781 acaaggcctt catcaccgag cgtctgatca actggacgct gagcgacacg accacgcgcg
     3841 tggtcatcaa ggtcggcgtc gcctaccgca acgatgcgcg tctcgcgcgg aagctgctgc
     3901 tgcaggtcgc cgaacaacac tcgctcgtgc tgcgcgaccc cgcgccgttg gcatggatga
     3961 ccagcttcgg cgattcgagt caggacttcg agctgcgcgt ctacgtcgcc gagatcaacc
     4021 agcgcaacct cgtgcgcaac gaactgcaga tgcgcatcgc cgaggtgttc cgcgaacacg
     4081 acatcgagat cgcctatccg acgcgcgacg tgtggttccg caatgcgctg gagacgaacg
     4141 gcgccgggcg cctcgaaaaa ccgtagcgag cgaagagccg atcctgtcta agatcgggcg
     4201 cgtcgccgga gcgcaggaac cggagcatac atacgagtat gtgaggattc cgagcaccgc
     4261 cggcgcgcaa attatcgagc gcagtaggtt tttcgaggcg ctaggtcagg ctgcgccgac
     4321 ggccgacgct agcagctcct cgaagcgcgc ctgatcgact tcgagcacga tctgcgcgtt
     4381 cgccgggtga cctaggcgat cctgccagtc gaccacggtc gcgccgcgcg tgagcgcgcc
     4441 gcc
//
LOCUS       DASRDG010000433         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_66403, whole genome shotgun sequence.
ACCESSION   DASRDG010000433 DASRDG010000000
VERSION     DASRDG010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66403"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VFA35_07215"
     CDS             <1..1024
                     /locus_tag="VFA35_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HZT55996.1"
                     /translation="VRQMLGAVDRGHAVRIVEAIAAGDGAALVGAVDGLRGLGLSATG
                     TLDEMAALLQQMAVLQAVPDAIDADDPDAPDAQRLAASIAADTTQLLYSIALHGRAEL
                     GLAPDEYSGLVMVLLRMLAFRPSDAAAPAPGPAAAPAPRPAQAPAQPAVVAPAPVLRV
                     AAPASEPPPWLDAPFDDEVREPAAVAREPEAAYAASPVASPVSAFTPTAVGARWAELV
                     RTLVEAGSISAFGRELALQAQCVALDEQAVPVVCRLRVEREALRAAAPCEKLQAALAE
                     ALQRSVRLEVEAGAADDSPAKRDAAERARRQAQAEQIIHDDPLVRALMAQYKTARIVP
                     GSVKPH"
     gene            1043..1369
                     /locus_tag="VFA35_07220"
     CDS             1043..1369
                     /locus_tag="VFA35_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZT55997.1"
                     /translation="MMKNQLAGLMKQAQAMQDNMKKAQEELALLEVEGQSGAGLVKVV
                     MTCKHDVKRITIDPSLLADDKDMLEDLVAAAFNDGVRRADEVSAEKMGKLTAGMPLPP
                     GMKFPF"
     gene            1384..1971
                     /gene="recR"
                     /locus_tag="VFA35_07225"
     CDS             1384..1971
                     /gene="recR"
                     /locus_tag="VFA35_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZT55998.1"
                     /translation="MAEPSVDALVDALRRLPGVGVKSAQRMAFHLLQHDREGAQRIAL
                     ALQSAVANVRHCERCHTFTERPVCSICLDGTRDARQLCVVETPADQAALERTGSYRGL
                     YFVLMGRLSPLDGIGSADIGAHELIARAADGHTEEVIIATNFTAEGEATAHVLGEALR
                     ARGLRVTRLARGVPIGSELEYVDLGTIAHALSDRR"
     gene            complement(1996..>3471)
                     /locus_tag="VFA35_07230"
     CDS             complement(1996..>3471)
                     /locus_tag="VFA35_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZT55999.1"
                     /translation="VAASAAAPQAAASAALPAPEVATAPVPVDPLRPEVPLNLDDRAA
                     QTDLWVRVRRGFAMPDLDTDLVRDREQWYATRPDYVQRMTERGGRYLFHIVDELERRG
                     MPTELALLPFIESAFNPQAVSSARASGMWQFMAATGKTFSLKQNIFRDDRRGVLQSTR
                     AALDYLQKLHGMFGDWQLALAAYNWGEGNVQRAIAKNQKLGLPTDYASLTMPNETRYY
                     VPKLQAVKNIVARPADFGLTLTPLKNHPYFLSVPIERDMDVAVAVKLAGMPLDEFQYL
                     NPQMNKPVILAAGTPQVLLPYDNANQFVRRLPLHRGPLASWTAWVAPKTVRPAVAAKE
                     VGMSEAQLREVNRIPPRMLVKAGSTLLVPRGERRQADVSLEIADNATMALAPDLPPLR
                     KMSLKAGRKETVAGVARRYRVSPAQVAQWNGTSAGASFRPGQTVIVYVAHKTGGHKGV
                     VTASTAHKGKAHAPSQHVRVATKGKASGKHASAGSSRVTRN"
BASE COUNT          473 a         1220 c         1291 g          487 t
ORIGIN      
        1 cgtgcgccag atgctcggcg cggtcgatcg cggccacgcg gtgcgcatcg tcgaggcgat
       61 cgcggcgggc gacggcgcgg cgctggtcgg cgcggtcgat gggctgcgcg ggctcgggct
      121 gtcggccacc ggcacgctcg acgagatggc cgcgctgctg cagcagatgg cggtgctgca
      181 agcggtgccc gacgcgatcg acgcagacga ccccgatgcg cccgatgcgc agcgcctggc
      241 cgcctcgatc gccgccgaca cgacccagct gctctacagc atcgcgctgc acggccgcgc
      301 cgagctcggg ctggcgccgg acgaatacag cggcctcgtg atggtgctgc tgcgcatgct
      361 cgcgttccgg ccgagcgacg cggccgcgcc cgcacccggc ccggccgcgg cgcctgcgcc
      421 gcgaccggca caggcacccg cgcagccggc cgtcgtcgcg cccgcgccgg tgctgcgcgt
      481 ggccgcgccc gcgagcgagc cgccgccgtg gctggacgca cccttcgacg acgaggtgcg
      541 cgagcccgcc gccgttgcgc gcgagcccga agccgcctac gccgcgtccc ccgttgcgtc
      601 gccggtgtcg gccttcacgc cgacggctgt tggcgcacgc tgggccgaac ttgtgcgcac
      661 gttggtcgag gccggcagca tcagcgcgtt cggacgcgag ctcgcgctgc aggcgcagtg
      721 cgtggcgctc gacgagcagg cggtgccggt cgtgtgccgc ctgcgcgtcg agcgcgaggc
      781 gctgcgcgcc gcggcgccgt gcgagaagct gcaggctgcg ctcgccgagg ccttgcagcg
      841 ctcggtgcgg ctcgaggtcg aggccggtgc cgccgacgac tcgcccgcga agcgcgacgc
      901 cgccgagcgc gcgcgccgcc aggcgcaagc cgaacaaatc attcacgacg accccttggt
      961 gcgggcgctg atggcgcagt acaagacggc acgcatcgtg ccggggtcgg tcaaaccgca
     1021 ttagttttcc aggagcaaga cgatgatgaa gaaccagttg gccgggctga tgaagcaggc
     1081 ccaggcgatg caggacaaca tgaagaaggc gcaggaagaa ctcgcgctgc tggaggtcga
     1141 gggccagtcg ggcgccggcc tcgtgaaggt ggtgatgacc tgcaagcacg acgtcaagcg
     1201 catcacgatc gacccgagcc tgctcgccga cgacaaggac atgctcgaag acctggtcgc
     1261 cgccgcgttc aacgacggcg tgcgccgcgc cgacgaggtc agcgccgaga agatgggcaa
     1321 gctgaccgcg ggcatgccgc tgcccccggg aatgaaattc ccgttctagg accagacgtt
     1381 ccgatggccg aaccatcggt cgacgcgctc gtcgacgcgc tgcgccgcct gcccggcgtc
     1441 ggcgtcaagt cggcgcagcg catggcgttc cacctgctgc agcacgaccg cgagggcgcg
     1501 cagcggatcg cgctggcgct gcagtcggcg gtggcgaacg tgcgccattg cgagcgttgc
     1561 cacaccttca ccgagcggcc ggtgtgcagc atctgcctcg acggcacgcg cgatgcgcgc
     1621 cagctctgcg tcgtcgagac cccggccgac caggccgcgc tcgagcgcac cggcagctac
     1681 cgcggcctgt acttcgtgct gatgggccgc ttgagcccgc tcgacggcat cggcagcgcc
     1741 gacatcggcg cgcacgagct gatcgcgcgc gcggccgacg gccacaccga agaagtgatc
     1801 atcgcgacca acttcaccgc cgagggcgag gcgaccgccc acgtgctggg cgaagcgttg
     1861 cgcgcgcgcg gcctgcgcgt cacgcggctg gcgcgcggtg tgccgatcgg cagcgagctc
     1921 gaatacgtcg acctgggcac gatcgcccac gcgctgagcg accggcgctg agcggcacgc
     1981 ggccgcgcgc cgcggtcagt tccgcgtgac gcgcgaagac ccggcgctgg cgtgcttgcc
     2041 gctcgccttg cccttggtcg ccacgcgcac gtgctgtgac ggcgcatgcg ccttcccctt
     2101 gtgcgcggtc gacgcggtca cgaccccctt gtgcccgccg gtcttgtgcg ccacgtagac
     2161 gattaccgtc tgccccggcc tgaagctcgc gccggcgctg gtgccgttcc attgggcgac
     2221 ctgggccggg ctgacgcggt aacgccgggc gacgccggcg acggtctcct tgcggccggc
     2281 tttcagcgac atcttgcgca gcggcggcag gtcgggcgcg agtgccatgg tggcgttgtc
     2341 ggcgatctcg agcgagacgt cggcctggcg gcgctcgccg cgcggcacga gcagggtcga
     2401 gccggccttc acgagcatgc gcggcgggat gcggttgacc tcgcgcagct gcgcctcgct
     2461 catgccgacc tctttggcgg cgacggccgg gcggacggtc ttgggcgcga cccaggcggt
     2521 ccagctcgcg agcgggccgc ggtgcagcgg caggcggcgc acgaactggt tcgcgttgtc
     2581 gtagggcagc agcacctgcg gcgtgcccgc cgcgaggatc accggcttgt tcatctgcgg
     2641 attgaggtac tggaactcgt cgagcggcat gccggcgagc ttgacggcga ccgccacgtc
     2701 catgtcgcgc tcgatcggca cgctcagaaa gtacgggtgg ttcttcaacg gcgtcagggt
     2761 caggccgaaa tcggccgggc gtgcgacgat gttcttcacc gcctgcagct tgggcacgta
     2821 gtaccgggtc tcgttcggca tcgtcagcga ggcgtagtcg gtcggcaggc cgagcttctg
     2881 gttcttcgcg atcgcgcgct gcacgttgcc ctcgccccag ttgtaggccg ccagcgcgag
     2941 ctgccagtcg ccgaacatgc cgtgcagctt ctgcaggtag tcgagcgcgg cgcgggtcga
     3001 ctgcagcacc ccgcggcggt cgtcgcggaa gatgttctgc ttcagcgaga aggtcttgcc
     3061 ggtcgcggcc atgaactgcc acatgcccga ggcgcgcgcg ctcgaaaccg cctgcgggtt
     3121 gaacgcgctc tcgatgaacg gcagcagcgc gagttcggtc ggcatgccgc ggcgttcgag
     3181 ctcgtcgacg atgtggaaca ggtagcggcc gccgcgctcg gtcatgcgct gcacgtagtc
     3241 ggggcgcgtg gcgtaccact gctcgcggtc gcgcaccagg tcggtgtcga ggtcgggcat
     3301 cgcgaagcca cggcgcaccc gcacccacag gtcggtctgg gccgcgcggt cgtcgaggtt
     3361 gagcggcacc tcggggcgca gcgggtcgac cggtaccggc gcggtcgcga cctcgggcgc
     3421 gggcagcgcg gcgctggccg cggcctgcgg cgcggcagcc gaggcggcca c
//
LOCUS       DASRDG010000434         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_178776, whole genome shotgun sequence.
ACCESSION   DASRDG010000434 DASRDG010000000
VERSION     DASRDG010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178776"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VFA35_07235"
     CDS             complement(<1..701)
                     /locus_tag="VFA35_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT56000.1"
                     /translation="MTAEPRLNHVQCLGARGLRRMAYWEWGDAANPRVLVCVHGLARQ
                     GRDFDTLARALSDAYRVVCPDVVGRGQSDWLTDPSGYQIPAYVADMVTLLARLNAPTV
                     HWVGTSMGGLIGLFLASLPQSPIGKLVINDVGPVVQFEALVRIGNYLGQPLRWNTLDE
                     ATDYLQTISTSFGPHTRAQWLAFSRPMLKADGDGFKLHYDPAIAQPFKAITPEIAAAG
                     EAMTWAAFDAIKCPTL"
     gene            complement(790..1530)
                     /locus_tag="VFA35_07240"
     CDS             complement(790..1530)
                     /locus_tag="VFA35_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014738.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZT56001.1"
                     /translation="MNFAPTSRRAAALALTFVLAAALAAPGSAATETANTAAPQGIVS
                     LSSSATLEVTRDLLSVVFNTSREGADAGAVQSQLKQALDAALAEAKKASRPGQLEVQT
                     GNFTISPRYTNKGVSNGWQGSAELIVEGRDMQAIGQLTGRITTLTISRVAYAISRELR
                     EKSEAEVSAQAIARYRAKAADYARQFGYAGYAIREVNVSSNEQPPMPRPMMMQAKAMS
                     APADEMLPVEPGKGTVTVNVSGTVQMTK"
     gene            1693..>2080
                     /locus_tag="VFA35_07245"
     CDS             1693..>2080
                     /locus_tag="VFA35_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZT56002.1"
                     /translation="MTAVANPRPDRIVVVDDDARIRDLLRRYLTQEGFEVLLAEDAKA
                     LNRLLTRETVHLIVLDLMLPGEDGLSICRRLRAANDVTPIIMLTAKVEDVDRIVGLEV
                     GADDYLPKPFNPRELLARIHAVLRRRP"
BASE COUNT          300 a          737 c          707 g          336 t
ORIGIN      
        1 agcgtcgggc acttgatcgc atcgaacgcg gcccaggtca tcgcctcgcc ggcagcagcg
       61 atctcgggcg tgatcgcctt gaacggctgc gcgatggccg ggtcgtagtg cagcttgaag
      121 ccgtcgccgt cggccttcag catcggccgc gagaacgcca gccactgcgc ccgcgtgtgc
      181 ggcccgaagc tggtcgagat cgtctgcagg tagtcggtcg cctcgtcgag cgtgttccag
      241 cgcagcggct ggccgaggta gttgccgatg cgcaccagcg cctcgaactg gaccaccggg
      301 ccgacgtcgt tgatcaccag cttgccgatc ggcgactgcg gcagcgaagc caggaaaagg
      361 ccgatcaggc cgcccatcga ggtgccgacc caatgcacgg tcggcgcgtt caggcgcgct
      421 agcaacgtca ccatgtcggc cacgtaggcg gggatctggt agccgctcgg gtcggtgagc
      481 cagtcggact gaccgcgccc gacgacatcg gggcagacca cgcgatacgc gtcgctcagc
      541 gcgcgcgcca gcgtgtcgaa gtcgcgcccc tgccgcgcca ggccatgcac gcagaccagc
      601 acgcgcgggt tcgccgcgtc accccactcc cagtacgcca tgcggcgcag gccgcgggca
      661 ccgagacatt ggacgtggtt caggcggggt tcggcggtca tggtcggctc tcgggtcggc
      721 tgttgcgctg tcggtgccgg gcatcgtaag cgaaggccgg cacccgctgc gccgccgcgc
      781 cgcgcgccct tacttcgtca tctgcaccgt gccgctgacg ttgacggtca ccgtgccctt
      841 gcccggctcg accggcagca tctcgtcggc cggtgccgac atcgccttgg cctgcatcat
      901 catcggccgc ggcatcggcg gctgctcgtt gctcgacacg ttgacctcgc ggatcgcgta
      961 gccggcgtag ccgaactgcc tcgcgtagtc ggccgccttc gcgcggtagc gcgcgatcgc
     1021 ctgcgccgag acctcggcct ccgacttctc gcgcaactcg cgcgagatcg cgtaggcgac
     1081 gcggctgatg gtcagggtcg tgatccggcc cgtcagctgg ccgatcgcct gcatgtcgcg
     1141 gccctcgacg atcaactcgg cgctgccctg ccagccgttg ctgacaccct tgttggtgta
     1201 gcgcggcgag atcgtgaagt tgccggtctg cacctcgagc tggcccggcc tcgaagcctt
     1261 cttcgcctcg gccagcgcgg cgtcgagcgc ctgcttcagc tgcgactgca ccgcgccggc
     1321 gtcggcgccc tcgcggctgg tgttgaagac gacgctgagc aggtcgcgcg tgacctcgag
     1381 cgtggcgctg ctcgacaggc tgacgatgcc ctgcggagcg gccgtgttcg ccgtctcggt
     1441 agcggcggac cctggcgccg ccagcgcggc ggcgagcacg aacgtgaggg ccagcgccgc
     1501 cgcgcggcgg ctcgtgggag cgaaattcat ggggcgatcc ttctgcagtc ggggtggcag
     1561 cttatacggc tgcgcctgcc gcaacggggt gaaaccgggc tgggacgcat ttgtaaccgc
     1621 tggtcagacc gacccaaaac gagccgtttt cgtgtccaaa atcggctctg ttacaaatct
     1681 ggagctcact gcatgaccgc tgttgccaat ccgcgccccg atcgcatcgt cgtcgtcgac
     1741 gacgacgccc gcatccgcga cctgctgcgc cgctacctga cgcaggaggg tttcgaggtg
     1801 ctgctggccg aggacgcgaa ggcgctgaac cgcctgctga cccgcgagac ggtccacctg
     1861 atcgtgctcg acctgatgct gcccggcgaa gacggcctgt cgatctgccg ccgcctgcgc
     1921 gccgcgaacg acgtgacgcc gatcatcatg ctgaccgcca aggtcgaaga cgtcgaccgc
     1981 atcgtcggcc tcgaggtcgg cgccgacgac tacctgccca agccgttcaa cccgcgcgaa
     2041 ctgctcgccc gcatccacgc ggtgctgcgg cgccggccga
//
LOCUS       DASRDG010000435         4325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_520612, whole genome shotgun sequence.
ACCESSION   DASRDG010000435 DASRDG010000000
VERSION     DASRDG010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520612"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFA35_07250"
     CDS             <1..918
                     /locus_tag="VFA35_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HZT56003.1"
                     /translation="VGTLLARRFHADGHEVVVLGRRPRAAPWRTAAWDAVHEGPWWRE
                     IDGADVVIGLAGRSVDCRYTATNRRLILDSRVQSAHAVATAIAASAQPPRVWLQASTA
                     TIYAHRYDAPNDEAAGRLGGDEAGAPPTWRFSTEVAKAWEAAANGRMLPRTRTVLMRS
                     AMTMSPDRGGIFDVLLRLVRFRLGGTPGDGRQYVSWIHADDFVRAVQWLIAHEGVAGP
                     VNMASPAPLPFADFLRELRSAWGTRIGLPAARWMLEIGAFVLRTETELILKSRRVAPG
                     RLLAGGFAFAFPDWPNAARDLCRRWRAQR"
     gene            complement(1012..1326)
                     /locus_tag="VFA35_07255"
     CDS             complement(1012..1326)
                     /locus_tag="VFA35_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56004.1"
                     /translation="MIRFQHPAVSLADLLEVRASLRRQEVLLKSALRDVQGELRCWNQ
                     HREAETEAGRPVARELCELWDELEGRERELAMDLVQVRAAWVTANAEIARMLKPPTTR
                     AS"
     gene            1546..2316
                     /locus_tag="VFA35_07260"
     CDS             1546..2316
                     /locus_tag="VFA35_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZT56005.1"
                     /translation="MRTRSRSAALLVLTLLPACKNLNLYSEAEEAKLGADAYKEETGK
                     YPAIASGPQYEMVQRVGHRIADATGKKYDWEFRLLKADDVVNAFCLPGGKVAVYTGIL
                     KVTQNENALAVVLGHEVAHATQRHGGKRMTEETLTQAALAAVDAGLSLSKMNPDAKQG
                     VMAAFGVGTQVGLTLPYSRAHETEADEVGIRYAIRAGYDPWEAPKLWERMAKLGDSGP
                     SWLSTHPASLDRAHDLERLIPQILAEERPGSPTTPPHK"
     gene            2445..3122
                     /locus_tag="VFA35_07265"
     CDS             2445..3122
                     /locus_tag="VFA35_07265"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZT56006.1"
                     /translation="MRHPRRARGFTLVELLIVVTILAIVAGIAIPSLMAAKSNANDKA
                     IVATMRAIATAQELARSHALIDTDKDGVGESATIPELAGAVAVRGTGTKLRPPYISWA
                     MGAQGPEGFVSSHGFYFALYLPDASGDGVIGAPASFASVNADLSEHCWTCVAWPINRA
                     TQGYATYFVNQNGDILCSRDARYSGKASVPPPGCALAGVAPNHIDTNALAVGVSGADG
                     NTWVPVH"
     gene            complement(3161..>4325)
                     /locus_tag="VFA35_07270"
     CDS             complement(3161..>4325)
                     /locus_tag="VFA35_07270"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HZT56007.1"
                     /translation="LNPIWNAWYFFTLYANADRVRGQFLTGQRGLLDRYALAKTRGFV
                     EGVQTAMDAYDLAAACQVAKDYLDALNNWYIRRSRPRFWGGTDAADRQDAFDTLYTCL
                     HVLCRAAAPLLPLLTEAVFRGLTGAESVHLQDWPDSAALPADAELVEQMDRVRAACSA
                     GLSLRESRKLRTRLPLQKAVLAGPAARSLAPYLPLVQDELNVKQVEIAERIDAFATQR
                     VQVDAKALGPRLGARMKDVLAATRAGAFRLLPGGRCELAGVELEPGEFMLQLVPKDGV
                     ACAALPQNDAVVVLDTAVTRALEEEGVARDFVRLVQQARKDARLHVSDRIALVVQTDA
                     DTERAIAAHQGYVMDQVLATALEFGTVSANLHAEKGAVGSGEGSEVVFGLRKT"
BASE COUNT          632 a         1572 c         1536 g          585 t
ORIGIN      
        1 gtcggcacgc tgctggcgcg gcggttccat gccgatgggc acgaggtggt cgtgctcggg
       61 cgccggccgc gcgcggcgcc ctggcgcacg gccgcgtggg acgccgtgca cgaagggccg
      121 tggtggcgcg agatcgatgg cgccgacgtc gtgatcgggc tcgccggccg ttcggtcgac
      181 tgccgctaca cggcgacgaa ccggcgcctg atcctcgact cgcgcgtgca gtcggcgcac
      241 gccgtcgcga ccgcgatcgc ggcaagcgcg cagccgccgc gcgtctggct gcaggcgagc
      301 acggcgacga tctatgcgca ccgctacgac gcgcccaacg acgaagcggc cggccggctc
      361 ggcggcgacg aggccggtgc gccgccgact tggcggttct cgaccgaggt cgccaaagcg
      421 tgggaggcgg cagcgaacgg gcggatgctg ccgcggacgc gcacggtgct gatgcgctcg
      481 gcgatgacga tgagccccga ccgcggcggg atcttcgacg tgttgttgcg gctggtgcgc
      541 ttccgcctcg gcggtacgcc cggcgacggg cgtcagtacg tgtcgtggat ccacgccgac
      601 gacttcgtgc gcgcggtgca gtggctgatc gcacacgaag gcgtcgcggg gcccgtcaac
      661 atggcttcgc ctgccccgct gccgttcgcc gacttcctgc gcgagctgcg ctctgcctgg
      721 ggcacgcgca tcggcctgcc ggccgcgcgc tggatgctcg agatcggcgc cttcgtgctg
      781 cgcaccgaga ccgaactgat cctgaagagc cggcgcgtcg cgcccggccg gctgctggcg
      841 ggcggcttcg cgttcgcgtt ccccgactgg ccgaacgccg cacgcgatct gtgccgccgc
      901 tggcgcgcgc agcgctagcg gcgccgcgcc cgtcgcgcgc cgcgaaggac tcgcccagtc
      961 aggagatcag cagtcagaag atcagcagtc agaggatcag cagccagaag atcagctggc
     1021 ccgggtcgtc ggcggcttca gcatgcgcgc gatctcggcg ttggcggtga cccaggccgc
     1081 gcgcacctgc acgagatcca tcgccagctc gcgttcgcgg ccttcgagct cgtcccagag
     1141 ctcgcacaac tcgcgcgcca cgggccgccc ggcctcggtc tccgcctcgc ggtgctggtt
     1201 ccagcagcgc aactcgcctt gcacgtcgcg cagcgcgctc ttcagcagca cctcctgccg
     1261 gcgcagcgac gcgcgaacct cgagcagatc ggcgagcgag acagcggggt gttggaaacg
     1321 gatcacgaca acctccagtg acggcctcac ggccgggtcg atgttgacca gtcgatactg
     1381 gccatctgag cggagcgagc ccgcaggtcg tcgatcgcga gatcggtcgg cagcaggctc
     1441 gctgccgaca gtcgtgagta gccgtggccg gcgaagaggt ttcagccggc gtgacaagaa
     1501 agcgtgaatc tggccccccg ccgggcgcgc agggatgatc ccgtcatgcg cactcggtcc
     1561 cggtccgcgg ccctgctggt cctcacgttg ctgcccgcct gcaagaacct gaacctctac
     1621 agcgaggccg aggaggccaa gttgggcgcc gacgcctaca aggaggagac gggcaagtac
     1681 cccgcgatcg cctcgggccc gcagtacgag atggtgcagc gcgtcggcca caggatcgcg
     1741 gacgcgacgg gcaagaaata cgactgggag ttcaggctgc tgaaggccga cgacgtcgtc
     1801 aacgcattct gcctgcccgg cggcaaggtc gccgtctaca ccggcatcct caaggtcacg
     1861 cagaacgaga acgcgctcgc ggtggtgctc ggccacgagg tcgcgcacgc gacgcagcgc
     1921 cacggcggca agcgcatgac ggaggagacg ctgacgcagg cggcgctggc cgcggtggac
     1981 gcggggctgt cgctgagcaa gatgaacccc gacgccaaac agggcgtgat ggccgcgttc
     2041 ggcgtcggca cgcaggtcgg cctcacgctt ccgtacagcc gcgcgcacga gaccgaggcc
     2101 gacgaggtcg gcatccgcta cgcgatccgc gccggctacg acccctggga ggcgcccaag
     2161 ctctgggagc gcatggccaa gctcggtgac agcgggccgt cgtggctcag cacgcacccc
     2221 gcgtcgctcg accgcgcgca cgacctcgag cgcctgatcc cgcagatcct ggccgaggag
     2281 cggcccggca gcccgaccac gccgccgcac aagtaacagt acaagtagca gcgccgcggc
     2341 gcttgaggct gccggcggcg cgctcggccc gttgccgccg gcgcgtcggc tgcgccgtga
     2401 gcccgatgcg ttgcctcctg ccgatagcaa ggctggagga agacatgcgc catccccgcc
     2461 gtgcccgcgg cttcacgctc gtggaactgc tgatcgtcgt gacgatcctc gcgatcgtcg
     2521 ccggcatcgc gatcccgagc ctgatggccg cgaagtcgaa cgccaacgac aaggccatcg
     2581 tcgcgacgat gcgggcgatc gccaccgcgc aggaactggc gcgctcccac gcgttgatcg
     2641 acaccgacaa ggacggcgtc ggcgaatcgg cgacgatccc cgagcttgcc ggcgccgtcg
     2701 cggtgcgcgg caccggcacc aagctgcgcc cgccgtacat ctcctgggcg atgggcgccc
     2761 aggggcccga aggcttcgtc agctcgcacg gcttctactt cgcgctctac ctgcccgacg
     2821 ccagcggcga cggcgtgatc ggcgcgcccg cgagcttcgc cagcgtcaac gccgacctgt
     2881 cggagcactg ctggacctgc gtcgcgtggc cgatcaaccg cgccacgcag ggctacgcga
     2941 cctacttcgt caaccagaac ggcgacatcc tgtgcagccg cgacgcccgc tactcgggca
     3001 aggcgagcgt gccgccgccg ggctgcgcgc tcgcgggcgt ggcgccgaac cacatcgaca
     3061 ccaacgcgct cgcggtcggc gtctccggcg ccgacggcaa cacctgggtg ccggtgcact
     3121 gatcgctgcg cggatacgca gcgatcaggg tgcgcgcccg ctacgtcttg cgcagcccga
     3181 acaccacttc gctgccctcg ccgctcccga cggcgccctt ctccgcgtgc aggttcgcgc
     3241 tcacggtgcc gaactcgagc gcggtggcga gcacctggtc catcacgtag ccctggtgcg
     3301 cggcgatcgc ccgctcggtg tccgcgtccg tctgcacgac cagcgcgatg cggtcgctca
     3361 cgtgcaggcg cgcgtccttg cgcgcctgct gcacgaggcg cacgaagtcg cgcgccacgc
     3421 cctcctcctc gagcgctcgc gtgacggccg tgtcgagcac caccaccgcg tcgttctgcg
     3481 gcagcgccgc gcacgcgacg ccgtccttcg gcaccagctg cagcatgaac tcgccgggct
     3541 cgagctcgac gcccgcgagc tcgcagcggc cgccgggcag cagccggaac gcgcccgccc
     3601 gggtcgccgc gagcacgtcc ttcatgcggg cgccgagcct cggcccgagc gccttcgcgt
     3661 cgacctgcac gcgctgggtc gcgaacgcgt cgatgcgctc ggcgatctcg acctgcttga
     3721 cgttgagctc gtcctgcacg aggggcaggt agggcgccag cgacctggcc gcggggccgg
     3781 cgagcaccgc cttctgcagc ggcaggcgcg tgcgcagctt cctgctctcg cgcagcgaca
     3841 ggccggccga acatgcggcg cgcacgcggt ccatctgctc gaccagctcc gcatccgcgg
     3901 gcagcgctgc tgagtccggc cagtcctgca ggtgcacgga ctcggcgccg gtgagcccgc
     3961 ggaacaccgc ctccgtgagc agcggcagca gcggcgcggc cgcgcggcag agcacgtgca
     4021 ggcaggtgta gagcgtgtcg aacgcgtcct ggcggtcggc ggcgtccgtc ccgccccaga
     4081 agcgcggccg gctgcggcgg atgtaccagt tgttgagcgc gtcgaggtaa tccttcgcga
     4141 cctggcacgc agccgcgagg tcgtaggcgt ccatcgccgt ctgcacgccc tcgacgaacc
     4201 cgcgcgtctt ggcgagcgcg tagcggtcga gcagcccgcg ctggccggtg aggaactggc
     4261 cgcgcacgcg gtcggcgttg gcgtagaggg tgaagaagta ccacgcgttc cagatcgggt
     4321 tgagg
//
LOCUS       DASRDG010000436         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_774039, whole genome shotgun sequence.
ACCESSION   DASRDG010000436 DASRDG010000000
VERSION     DASRDG010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774039"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VFA35_07275"
     CDS             <1..1159
                     /locus_tag="VFA35_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZT56008.1"
                     /translation="GDEGLEKISSFRPSIVVTDLVMPRKTGLELLEVVRDMDSAIAVI
                     LLTAQGTVESAVEAIRNGAYDYLTKPVDPQRLRILLDKAAERQHALREVRALRRQLRD
                     RGAFGGMIGASPEMRKIYQVVEQAAPTTASVLITGESGTGKELVAQTIHALSPRGQMP
                     YVPINCAAIPETLLESEIFGHEKGAFTGAMERRQGVFELADRGTIFLDEIAEMTPQTQ
                     VKLLRVLQERRFRRLGGRVETPVDVRVVAATNQDPLQAVRDNKLREDLFYRLNVFAIA
                     LPPLRDRKEDLPLLTQAFIAEFADRNGKAVKAISGEAMRKLEQYHWPGNVRELRNVIE
                     RATILAEGEFIELKHLPPLGVVPVAAVGSGMTLEAGITVEEAERRLIVLTL"
     gene            1330..>2252
                     /locus_tag="VFA35_07280"
     CDS             1330..>2252
                     /locus_tag="VFA35_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56009.1"
                     /translation="MRVSLRTKQVLGVTALVGAVVVGLSVHDLAAQARVRLEESDARG
                     QLLARAIYQRAFAVIPDALDPYAALRDDGGVRAILESSIYSKGVTYAAIVDATGSVIA
                     QYPPEQISSPMREAVPLLGDFVKQGAIAQLREIFFGDGRTFEVREDLKIGGVNAVTRI
                     GVSTLLVRSELQKALYPVLVTAGYLLIGATLGALLLSQWLLRPIHVLRSGLRRLERGE
                     PAAALDLPQDEFGEMGQEIRAVSEKLLAGREATGPEAGLDSIVEHLADAVGIFGPSGD
                     VLFANPALRALLAACEREGSKPFDGLVDHTH"
BASE COUNT          374 a          747 c          784 g          347 t
ORIGIN      
        1 cggcgacgaa gggctcgaga agatcagcag cttccggccc agcatcgtgg tcaccgacct
       61 ggtgatgcct cgcaagaccg ggctcgagct gctcgaagtg gtgcgcgata tggattcagc
      121 cattgccgtg atactgctga cggcgcaagg gacagtcgag tcggcggtgg aagccattcg
      181 caacggcgcg tacgactatc tcaccaagcc ggtcgacccg cagcgtcttc gcattctgct
      241 cgacaaggct gccgaacgcc agcacgcgct gcgcgaagtg agggcgctgc gccgccagct
      301 ccgcgatcgc ggcgccttcg gcggcatgat cggcgccagc cccgagatgc ggaagatcta
      361 ccaggtggtg gaacaggcgg cgccgaccac cgcgtccgtg ctgatcacgg gcgagtcggg
      421 cacgggcaag gagctggtgg cgcagaccat tcacgcgctg agcccgcgcg ggcagatgcc
      481 gtacgtgccg atcaactgtg ccgcgattcc cgaaacgctc ctggagagcg agatcttcgg
      541 gcacgagaag ggcgcgttca ccggcgcgat ggagcggcgg cagggcgtct tcgagctcgc
      601 cgaccgcggc acgatcttcc tggacgaaat tgcggagatg acgccgcaga cccaggtgaa
      661 gctgcttcgc gtgctgcagg aacgccgctt ccggcgcctc ggcggccgcg tcgaaacgcc
      721 ggtggacgtg cgcgtggttg cggccaccaa ccaggatccg ctgcaggccg tccgcgacaa
      781 caagctgcgc gaagacctgt tctatcggct caacgtcttt gccatcgcgc tgccgccgct
      841 gcgcgaccgc aaggaggatt tgccgctgct cacgcaggcc ttcatcgccg agttcgccga
      901 tcgcaacggg aaggccgtga aggcgatcag cggcgaggcg atgcgcaagc tggagcagta
      961 tcattggccc ggcaacgtgc gcgagctgag gaacgtaatc gagcgcgcga cgatcctcgc
     1021 cgagggcgag ttcatcgagt tgaagcacct gccgcctctc ggggtcgtgc ccgtggcggc
     1081 tgttggcagc ggcatgacgc tcgaggccgg catcacggtc gaagaggccg agcggcgcct
     1141 gatcgtgctg acgctctaac acacgcgcga caacaagacg cgcgcggccg agattctcgg
     1201 gatcagcctg aagacgctgc acaacaaact gaacaggctc ggcctcagga agggcctctg
     1261 atcgccgagc gaacccactg atcgccaggc gcgtggcgct ggcggcccga ggctccccgg
     1321 ccccctgaca tgcgcgtttc ccttcgcacc aagcaggttc tcggcgtcac cgccctggtc
     1381 ggcgccgtgg tggtcgggct cagcgtgcac gacctggccg cgcaggcgcg cgtcaggctg
     1441 gaagaaagcg atgcgcgcgg gcagttactc gcccgcgcga tctatcagcg cgccttcgcc
     1501 gtgatcccgg acgccctgga tccctacgcg gcgctccgag acgacggcgg tgtgcgcgcg
     1561 atcctcgagt cttccatcta ctcgaagggc gtcacctacg cggcgatcgt cgacgccacc
     1621 ggcagcgtga tcgcgcagta tccgcccgag cagatctcga gcccgatgcg ggaagccgtg
     1681 ccgctgctgg gcgacttcgt caagcagggc gcgatcgcgc agctccggga gattttcttc
     1741 ggcgacggcc gcacgttcga ggtccgcgag gacctgaaga ttggcggcgt gaacgccgtc
     1801 acccgcatcg gcgtctccac gcttctcgtg cggagcgagt tgcagaaggc gctctatccg
     1861 gtgctcgtca cggccggcta tctgctcatc ggcgccacgc tgggcgcctt gctgctgtcg
     1921 cagtggctgc tccggcccat ccacgtgctg cgcagtggac ttcggcgact ggaacgcggc
     1981 gagcccgcgg cggcgctcga cctgccgcag gacgagttcg gcgagatggg ccaggagatt
     2041 cgcgcggtga gcgagaagct gctcgccggt cgcgaagcga cggggcctga agccggcctc
     2101 gactcgatcg tcgagcacct ggctgatgcg gtgggcatct tcggcccgag cggggacgtg
     2161 ctgtttgcca accctgcgtt gcgcgcgctg ctggccgcct gcgaacgcga gggcagcaag
     2221 ccgttcgacg ggctcgtgga tcacacgcac ga
//
LOCUS       DASRDG010000437         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_637615, whole genome shotgun sequence.
ACCESSION   DASRDG010000437 DASRDG010000000
VERSION     DASRDG010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637615"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..694
                     /locus_tag="VFA35_07285"
     CDS             185..694
                     /locus_tag="VFA35_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HZT56010.1"
                     /translation="MMNSIAKADTRRESVNPGADWGDGEPAEAFKPWSREEAQVWRKS
                     NPSSSPWRVVAAQAVVGLVCAAVAWAFTRQGGAAWSALYGAAAVVLPSALLARGMTKR
                     ARNPLAMAAGFMFWEMLKIGVAIAMLAIAMRVVPNLSWPALLVTMVVCIKVNWVALLW
                     RGRRVDNKA"
     gene            701..1561
                     /gene="atpB"
                     /locus_tag="VFA35_07290"
     CDS             701..1561
                     /gene="atpB"
                     /locus_tag="VFA35_07290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZT56011.1"
                     /translation="MAAEAVAKEGAAHAPTAGEYIIHHLQHLQNKHQHGVADFSVVNL
                     DSMFFTIVIGALGCWLLWLAARKATSGVPGRFQAAVEILVEMVDSQAKGIVHNANSRK
                     LVAPLALTVFVWIFLLNAMDLLPVDLLPWIGEHVFGLQHLRVVPTADLSVTMGLSVGV
                     LIVCLVYNVKIKGAGGWVHELFSAPFGDKWFLYPVNFLMQVIEFVAKTVSHGMRLFGN
                     MYAGELIFMLIALMGGAFALSASGILLTIGHIIAGSVWAIFHILIITLQAFVFMMLTL
                     VYVGQAHDSH"
     gene            1664..1930
                     /gene="atpE"
                     /locus_tag="VFA35_07295"
     CDS             1664..1930
                     /gene="atpE"
                     /locus_tag="VFA35_07295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZT56012.1"
                     /translation="MMEVISFVALAAGLIIGLGAVGACIGIGIMGSKYLESAARQPEL
                     MNELQTKMFLLAGLIDAAFIIGTGIALWFATANPFLAQLANLPK"
     gene            1995..2465
                     /locus_tag="VFA35_07300"
     CDS             1995..2465
                     /locus_tag="VFA35_07300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZT56013.1"
                     /translation="MSINATLIVQMIVFLILVGFTMKFVWPPIVKALDERAKKIADGL
                     SAADKAKAELASANKRVEEQLQAARKDATQRLADAERLAQSMIEEAKGRATEEGAKII
                     ASAKAEAAQESIKARDALRDQVAALAVKGAEQILRKEVNPAVHASLLSQLKTEL"
     gene            2525..3058
                     /locus_tag="VFA35_07305"
     CDS             2525..3058
                     /locus_tag="VFA35_07305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZT56014.1"
                     /translation="MAELATLARPYAEALFQVAAKGDLAQARAELDALASVAANPQLR
                     QFADAPTAGAQQVFDVITGVANLPLSDASKNLLRTVIDNGRLSVLPEIATQFHALVSE
                     RSGVADAVVYSAFEIEPAQLADLVAALQTRFKRKLNATVQVQPDLIGGIRVVVGDEVL
                     DTSVKARLQQMKATLTA"
     gene            3105..>3306
                     /locus_tag="VFA35_07310"
     CDS             3105..>3306
                     /locus_tag="VFA35_07310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808279.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZT56015.1"
                     /translation="MNLNPAEISELIKSRIEGLGPTMDIRNQGIVVSVTDGICRVHGL
                     SDAMQGEMLEFPPSADGTPSFGL"
BASE COUNT          576 a         1068 c         1066 g          596 t
ORIGIN      
        1 gaggaactcg gccgcggttt cgatgccgcc gggtcggccg cttcgttcaa ggcactcgca
       61 gcgcactacc tgcaactgcc ctgagccgtc aatcaagtcc gcacgttgcg gcggaggccg
      121 tccgaagtct gggtggcttc ccctataatt ccacggcttt gctgcgtgtg caccctgtca
      181 ggccatgatg aactcgatag cgaaagccga cacccgtcgc gagtccgtca accccggcgc
      241 agattgggga gatggggagc ccgccgaagc gttcaagccc tggtcccgcg aagaggccca
      301 ggtttggagg aagagcaacc cgtcgtcatc gccttggcgg gtcgtcgcgg cacaggccgt
      361 ggtggggctg gtgtgtgccg cagtcgcctg ggcattcacc cgccagggcg gggcagcctg
      421 gtcagcgctg tatggcgcgg cggctgtcgt cttgccgagt gcgctgctgg cgcgcgggat
      481 gacgaagaga gcgcgaaacc cgctggccat ggccgcgggc ttcatgttct gggagatgct
      541 gaaaataggg gtggcgatcg cgatgttggc gatcgccatg agggttgtac cgaatctgag
      601 ctggccggca ctgctggtca cgatggtcgt gtgcatcaag gtcaactggg tcgcgctgct
      661 gtggcgcggc cggcgtgtgg acaacaaggc gtagaacaag atggcagcag aggcagtggc
      721 gaaagaaggc gcggcgcatg cccccaccgc aggtgaatac atcattcatc acctgcagca
      781 cctgcagaac aagcaccagc acggggtggc cgacttctcg gtcgtcaacc tcgactcgat
      841 gttcttcacg atcgtcatcg gtgcgctcgg ctgctggctg ctctggctgg ctgcgcgcaa
      901 ggccacctcg ggcgtgccgg gccgcttcca ggccgcggtc gagatcctgg tcgagatggt
      961 cgacagccag gccaagggca tcgtgcacaa cgccaacagc cgcaagctgg tcgcgccgct
     1021 ggcgctcacg gtgttcgtct ggatcttcct gctcaacgcg atggacctgt tgccggtcga
     1081 tctgctgccg tggatcggcg agcacgtgtt cggcctgcag cacctgcgcg tcgtgcccac
     1141 cgccgacctc tcggtgacga tgggcctgtc ggtcggcgtg ctgatcgtct gcctggtcta
     1201 caacgtcaag atcaagggcg cgggcggctg ggtgcacgag ttgttctcgg cgccgttcgg
     1261 cgacaagtgg ttcctgtacc cggtgaactt cctgatgcag gtcatcgagt tcgtcgcgaa
     1321 gaccgtctcg cacggcatgc ggttgttcgg caacatgtac gccggcgaac tgatcttcat
     1381 gctgatcgcg ctgatgggcg gtgcgttcgc gctgagcgcc agcggcatcc tgctgaccat
     1441 cggccacatc atcgccggct cggtctgggc gatcttccac attctgatca tcacgctgca
     1501 ggcattcgtg ttcatgatgc tgacgctggt gtacgtcggc caggcacacg acagccacta
     1561 gccttcaatc gctcttttcc ccacggcgtc tctctccttt ccagttcgtc atcttcgaga
     1621 ctcgtcttct ttcccttctc ttctaacaac ctcaattgga gtcatcatgg aagtcattag
     1681 ctttgttgcc ctcgccgctg gcctgatcat tggtctgggt gccgtcggcg cctgtatcgg
     1741 catcggcatc atgggcagca agtacctcga gtcggccgcg cgccagcccg agctgatgaa
     1801 cgaactgcaa accaagatgt tcctgctggc cggcctgatc gatgccgcgt tcatcatcgg
     1861 caccggtatc gcgctgtggt tcgccaccgc caacccgttc ctggcgcagc tcgccaacct
     1921 gccgaagtaa gccgttgcgg cgccggcatg accggtgtct tgcaatcccc ctttcttcct
     1981 acagaggcaa gaacgtgagc atcaacgcaa cgctgatcgt gcaaatgatc gtgttcctga
     2041 tcctcgtcgg cttcacgatg aaattcgtgt ggccgccgat cgtgaaggca ctcgacgagc
     2101 gcgcgaagaa aatcgccgat ggcctgtcgg ccgccgacaa ggccaaggcc gagttggcca
     2161 gcgccaacaa gcgcgtcgaa gagcaactgc aggccgcccg caaggacgcg acgcagcgcc
     2221 tggccgacgc cgagcgcctc gcccagtcga tgatcgaaga ggccaagggc cgcgcgaccg
     2281 aagagggcgc gaagatcatc gcctcggcca aggccgaagc cgcgcaggag tcgatcaagg
     2341 cgcgcgacgc gctgcgcgat caggtcgccg cgctggccgt caagggcgcc gagcagatcc
     2401 tgcgcaagga agtgaacccc gcggttcacg cctcgctgct gtcgcaactg aagaccgagc
     2461 tatgagcgac gcagttcgct cccatcgaat gagcgacgca gttcgctcct acggagcctg
     2521 accgatggct gagcttgcaa cccttgcccg gccgtatgcc gaagcgctgt tccaggtcgc
     2581 cgcgaagggc gacctggcgc aggcccgtgc cgagctcgac gcgctggcgt cggtggccgc
     2641 gaacccgcag ctgcgccagt tcgccgacgc gccgaccgcc ggcgcgcaac aggtgttcga
     2701 cgtgatcacc ggcgtggcga acctgccgct ctcggacgcg tcgaagaacc tgctgcgcac
     2761 cgtgatcgac aacggccgcc tgagcgtgct gcccgagatc gccacgcagt tccacgcgct
     2821 ggtgagtgag cgctcgggcg tggcggatgc ggtcgtctac agcgcgttcg agatcgagcc
     2881 ggcgcaactg gccgacctcg tcgccgcgct acagacccgc ttcaagcgca agctcaacgc
     2941 caccgtgcag gtgcagcccg atctgatcgg cggcattcgc gtggttgtcg gcgacgaagt
     3001 gctcgacacc tcggtgaagg cacgcctcca acaaatgaaa gcgacgctca cggcctgagc
     3061 gccgtgatcg acgttcgccg gacctcctcg aatcggagac agtgatgaac ctgaaccccg
     3121 cagaaatttc cgaactgatc aagagccgca tcgagggcct cggcccgacg atggacatcc
     3181 gcaatcaggg catcgtcgtg tcggtcaccg acggcatctg ccgcgtgcac ggcctgtcgg
     3241 acgcgatgca gggcgaaatg ctggaattcc cgccgagcgc ggacggcacg ccttcgttcg
     3301 gcctgg
//
LOCUS       DASRDG010000438         4434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_521209, whole genome shotgun sequence.
ACCESSION   DASRDG010000438 DASRDG010000000
VERSION     DASRDG010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521209"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFA35_07315"
     CDS             <1..332
                     /locus_tag="VFA35_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HZT56016.1"
                     /translation="GCRARRCAAAAFDRLTLPGPQLGGRGRVRAWFSRGSVMKQRVIV
                     IDREIQSGAPVFAGTRVPVKILFDYLEAGDSLDVFLEEFPSITREMAVAALEEAQSAL
                     IHDAHPA"
     gene            complement(479..1267)
                     /locus_tag="VFA35_07320"
     CDS             complement(479..1267)
                     /locus_tag="VFA35_07320"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HZT56017.1"
                     /translation="MILNVLGCSGSIAAGCKTTAFLLDGDVLIDAGTGVGDLELEALA
                     RVDHILISHSHLDHVLSIGLLADAVMRLRTAAGRGPIQVHALPETIAALRTHIFNGVI
                     WPDFTRLPSAENPVLELVPFAVGDVLELNGKRIEVLSASHTVPAVGFAVATGPGDDAA
                     WWVYTGDTGPNPALWERLRRMKVADLIIETAFSDEERQLARISRHLCPTALGHELQQL
                     SGSVDVHITHIKPGESEAVMAEIGKLGSPHRIHALAAGQVMRLE"
     gene            complement(1264..3516)
                     /locus_tag="VFA35_07325"
     CDS             complement(1264..3516)
                     /locus_tag="VFA35_07325"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZT56018.1"
                     /translation="MTSRSGLWRALRALLLLAAVLAALGHAAGWHPLRFVTELDLAIA
                     DARLRAFMPRTADPRIVVVDVDEKSLAEVGRWPWSRDRLAALVDELFERQQIAVLGFD
                     VLFAEPDISSGLPALERLAAASPAIAAAIAPLRPALDFDARFARALAGRNVVLGYYLG
                     NARERRSGVLPAPVFDAALLQGRPIAFTHWDGYAANLAVFARAAPLAGYFNNVPDPDG
                     LVRAVPLISELDGRLYEPLALAVFRAYTGGPSVLPGLPQERWLSRQYSALQSVLLRQG
                     DALQAIPVDANARVRVPFRGPGGPRGGSFEYVSASDLLNQRVAPGHLRGKLVLVGSTA
                     PGNYDQRATPVADVYPGAEVHANLLSGLLDNRLPVQPDWAGAFEVLQLLGVAGLLAAA
                     LPRLRAAHGAQLAGALGVALVAINLWAYRFEGLLLPLASALLLTGVIYVGTTVWGYIV
                     EGRSRRSLARLFGTYVPPELVEEMARDPARYDMRAENRELTVMFCDMRNFTRVSELLP
                     PEDVRGLVNSFFSTMTAAIRAQRGTLDKYIGDAIMAFWGAPVSDPAHAANAVRAALSM
                     SARLAALNAELRARKLPEIGLGIGLNTGTVCVGDMGSSMRRSYTVMGDAVNLAARIEA
                     LTRHYGVDILVGEDTRRAAGDLWHWVEVDRVRVKGKRKPVTLFTPVALPAERSIPFDE
                     EKRLWQLALASYRLQHWDETQARLQGLLTAFADTPFAGLYRQLGERTDHYRTAPPPAD
                     WDGAHTFDSK"
     gene            complement(3562..4239)
                     /locus_tag="VFA35_07330"
     CDS             complement(3562..4239)
                     /locus_tag="VFA35_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZT56019.1"
                     /translation="MGKLVVSLDGVVIKEVQITKDKTTLGRRPYNDIVIDNLAVSGEH
                     AVLQMVGADVFIEDLNSTNGTYINGKAVKKQLLSHNDTVEVGKYKIKFMVDDGTDYEK
                     TMIMKPGTRPPAPPGQPQPPLNSGFGASGFGALGGSSVPASIRVLNGAAAGREVALTK
                     VVTTVGKPGVQVASITKRPGGYVLAHVEGTLQPTVNGNPVVGETVHLKNGDVIELAGT
                     QMQFVQA"
BASE COUNT          658 a         1480 c         1606 g          690 t
ORIGIN      
        1 aaggatgccg tgcgcggcgg tgcgcggcgg ctgcatttga tcgattgacg ctgcccggcc
       61 cccaactcgg cggtagaggt agagtgcgag catggttcag ccggggcagc gtgatgaagc
      121 aacgtgtcat cgtcatcgat cgcgagattc agagtggggc gccggtcttc gcaggtacgc
      181 gagtgcccgt gaaaatcctg ttcgactact tggaagccgg cgattctctt gatgtgtttc
      241 tcgaggagtt tccttccatt acgcgcgaga tggctgtcgc cgccctcgag gaggcgcagt
      301 ccgcgctgat tcacgatgcg catcctgctt gacgagtcac tgccgaaggc actccgcggt
      361 actggtgctc gtcgtgagga acaaccggat cgaatcgatt gatccgctga tcccggagct
      421 gccttccact cttgagaatc atgcgccgcg cagcttgcac aggatcggcg tttgaccctc
      481 actcgagccg catcacctgc ccggccgcca gcgcatgaat ccgatgcggc gaccccagct
      541 ttccgatctc cgccatcacc gcctcgcttt ccccgggttt gatgtgggtg atgtggacgt
      601 cgacgctccc tgagagctgc tgcagctcgt gccccagcgc cgtcgggcac aggtggcggc
      661 tgatgcgggc gagctggcgc tcttcgtcgc tgaacgcggt ctcgatgatc aggtcggcga
      721 ccttcatccg gcgcaggcgc tcccaaagcg ccgggttggg gccggtgtcg ccggtgtaga
      781 cccaccaggc ggcgtcgtcg cccgggccgg tggcgacggc gaagccgacc gccggcacgg
      841 tgtgcgaggc gctgagcacc tcgatgcgct tgccgttgag ttcgagcacg tcgcccacgg
      901 cgaacggcac gagttcgagc acggggttct ctgcgctggg caggcgggtg aagtcgggcc
      961 agatcacgcc attgaagatg tgggtgcgca gcgccgcgat ggtctcgggc agcgcgtgca
     1021 cctggatcgg cccgcggccg gcggcggtgc gcaggcgcat cacggcgtcg gcgagcaggc
     1081 cgatcgagag cacgtggtcg aggtgcgaat ggctgatcag gatgtggtcg acgcgcgcga
     1141 gcgcctcgag ttcgaggtcg ccgacgccgg tgcccgcgtc gatcagcaca tcgccgtcga
     1201 gcaggaacgc cgtggtcttg cagcccgcgg cgatggaccc ggaacaaccg agcacgttga
     1261 ggatcatttg ctgtcgaagg tgtgggcgcc atcccagtcg gcaggtggtg gcgcggtgcg
     1321 gtagtggtcg gtgcgttcgc cgagttggcg gtagaggccc gcgaaaggcg tgtcggcaaa
     1381 cgccgtcagc agcccttgca agcgggcctg cgtctcgtcc caatgttgca gccggtacga
     1441 ggccagcgca agctgccaaa gtcgcttttc ctcgtcaaac ggtattgatc gttcggcggg
     1501 cagcgcgacc ggcgtgaaaa gcgtcacggg cttgcgtttg cccttcacgc gcacgcgatc
     1561 gacctcgacc caatgccaca ggtcgccggc agcgcggcgg gtgtcttcgc cgaccaggat
     1621 gtcgacgccg tagtgccgcg tcagcgcctc gatgcgcgcg gccaggttca ccgcgtcgcc
     1681 catcacggtg tagctgcgcc gcatgctcga gcccatgtcg ccgacgcaga cggtgccggt
     1741 gttcaggccg atgccaaggc cgatctcggg cagcttgcgc gcgcgcagct cggcgttcag
     1801 cgcggccagc cgcgcgctca tcgaaagcgc ggcccggacg gcattcgcgg cgtgcgccgg
     1861 gtcgctgacc ggcgcgcccc agaaggccat gatggcgtcg ccgatgtact tgtcgagcgt
     1921 gccgcgctgc gcgcggatcg ccgcggtcat ggtcgagaag aagctgttga cgaggccgcg
     1981 cacgtcttcg ggcggcagca gctccgagac ccgcgtgaag ttgcgcatgt cgcagaacat
     2041 cacggtcagc tcgcggttct cggcgcgcat gtcgtagcgc gccgggtcgc gcgccatttc
     2101 ctcgacgagc tcgggcggca cgtaggtgcc gaacagccgg gccagcgagc gccggctgcg
     2161 gccttcgacg atgtagcccc agaccgtcgt gccgacgtag atcaccccgg tcagcagcag
     2221 cgccgacgcg agcggcagca gcagcccctc gaagcgatag gcccacaggt tgatcgcgac
     2281 cagcgcgacg ccgagcgcgc cggcgagttg cgcgccgtgc gcggcgcgca ggcgcggcag
     2341 ggccgcggcg agcagcccgg cgaccccgag cagctgcagc acctcgaagg cgccggccca
     2401 gtcgggctgc accggcaggc ggttgtcgag caggcccgag agcaggttcg cgtgcacctc
     2461 ggcgccgggg tagacatcgg ccaccggcgt cgcacgctgg tcgtagttgc cgggggcggt
     2521 gctgcccacc agcaccagct tgccccgcag gtggccgggc gcgacgcgct ggttcagcag
     2581 gtcgctcgcc gagacgtact cgaacgagcc cccgcgcggc ccgccgggcc cgcggaacgg
     2641 cacgcgcacg cgcgcgttcg cgtcgaccgg aatcgcctgc agtgcgtcgc cctgcctcag
     2701 cagcacgctt tgcagcgcgc tgtactgccg gctcagccag cgctcctgcg gcaagccggg
     2761 cagcaccgac ggcccgccgg tgtaggcgcg gaacaccgcg agcgccagcg gctcgtagag
     2821 ccggccgtcg agttcggaga tcagcggcac cgcgcgcacc aggccgtcgg ggtcgggcac
     2881 gttgttgaag tagccggcca gcggcgcggc gcgcgcgaac acggccaggt tcgcggcgta
     2941 gccgtcccag tgcgtgaacg cgatcggccg gccctgcagc agcgccgcgt cgaagaccgg
     3001 cgcgggcagc acgccgctgc ggcgctcgcg cgcattgccg aggtagtagc cgaggacgac
     3061 gttgcggccc gcgagcgcgc gggcgaagcg cgcgtcgaag tcgagtgccg ggcgcagcgg
     3121 cgcgatcgcg gcggcgatgg cgggcgaagc ggcggccagc cgctcgagcg ccggcaggcc
     3181 cgagctgatg tcgggctcgg cgaacagcac gtcgaagccg agcaccgcga tctgttgcct
     3241 ctcgaacagc tcgtcgacca gcgccgcgag ccggtcgcgg ctccacggcc agcggccgac
     3301 ctcggcgagg cttttctcgt cgacatcgac gacgacgatg cgcgggtcgg ccgtgcgcgg
     3361 catgaaggcg cgcaagcggg cgtcggcgat cgcgaggtcg agctcggtga cgaagcgcag
     3421 cgggtgccag ccggcggcgt ggccgagcgc ggcgagcacc gcggccagca gcagcagcgc
     3481 gcgcagagca cgccacaggc ccgagcgcga ggtcatgcgg gggagcgggt cagccgcgcg
     3541 ggctgcgcgg ccgagtgagg gtcaagcctg gacgaactgc atctgcgtgc cggcgagctc
     3601 gatcacgtcg ccgttcttca ggtgcacggt ctcgccgacc accgggttgc cgttgacggt
     3661 gggctgcagc gtgccctcga cgtgcgcgag cacgtagccg cccggccgct tggtgatcga
     3721 ggcgacctgc acgccgggct tgccgacggt ggtcacgacc ttggtcagcg ccacttcgcg
     3781 gcctgccgcg gcgccgttga gcacgcggat cgaggccggc accgacgacc cgccgagcgc
     3841 gccaaagccc gaggcgccga agcccgaatt cagcggcggc tgcggctgcc ccggcggcgc
     3901 gggcgggcgc gtgccgggct tcatgatcat ggtcttttcg tagtcggtgc cgtcgtcgac
     3961 catgaacttg atcttgtact tgccgacctc gaccgtgtcg ttgtggctga gcagctgttt
     4021 cttgacggcc ttgccgttga tataggtgcc gttggtgctg ttcaggtctt cgatgaagac
     4081 atcggcaccg accatctgca gcacggcgtg ctcgccgctg accgccaggt tgtcgatcac
     4141 gatgtcgttg tagggccggc ggccgagcgt ggtcttgtcc ttggtgatct gcacttcctt
     4201 gatcaccaca ccatcgagcg atacgaccag tttgcccatg cttttgacct cttgtgtctg
     4261 agtcttcgac ggttggcggc tcgctgccgc acgcaggttt cagcggcgga agggccacca
     4321 ggaccgcgcc gggccggtgc tcccacgaac acgcgccaga acgaccgaaa tattatcctt
     4381 gccgccggcc tcgttggcag cgtcgatcaa agcaccggcg gcttcgggca gggt
//
LOCUS       DASRDG010000439         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_180240, whole genome shotgun sequence.
ACCESSION   DASRDG010000439 DASRDG010000000
VERSION     DASRDG010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180240"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFA35_07335"
     CDS             complement(<1..467)
                     /locus_tag="VFA35_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HZT56020.1"
                     /translation="MRTFTLNAVALALLAMVGAAHAQTATELNKVTVTGEGDKLGTGL
                     LMDEDAPKARSSVTKAQLEKTRSSGNPFQALSLLPGVNSSSVDATGLFGGNLRVRGFN
                     SDQMGFTINGAPVNDSGSFSVFPQEYVDSENLCSLYVTQGAPDTDAPHVGASGG"
     gene            complement(756..1727)
                     /locus_tag="VFA35_07340"
     CDS             complement(756..1727)
                     /locus_tag="VFA35_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZT56021.1"
                     /translation="MRRPAEMTTKILFDTDPGIDDAMALLMLARDRRAELLGITTVFG
                     NAAVELTTANALALCERFGIEAPVARGAAQALRRPPGGFPTEVHGIDAMGDIGMPPAR
                     TRRALPQPAPQFIAELARRHAGELTLVAVGPLTNLALALAHDAALVDHVKAVVVMGGA
                     FGENGHRGNVSPVAEANIAADPHAADRVFAARWPVTIVGLDVTQEVLLDTAWLDALGR
                     EGAAEGAFIREITRHYEDFYRPRTGGGVFSHDASAVACALDATAFTLRRGPVRVVTEG
                     IAAGQTIQVREGQRFPSTAWDDAPAQSVCVAVDAPRLRAEFRACFVR"
     gene            complement(1724..2932)
                     /locus_tag="VFA35_07345"
     CDS             complement(1724..2932)
                     /locus_tag="VFA35_07345"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HZT56022.1"
                     /translation="MTRALVIVLDSFGLGAAPDAPRFGDVGADTFGHIAQWCAAHGRP
                     LRLPNLARLGLPQAAHAASGRWPEGVVVDPAPQGAYGAAAETSLGKDTPSGHWELMGC
                     PVAFDWGYFATSGDPAVPVFPPALMQRWLAACGLAGSLGNCHASGTEIIERLGDEHLR
                     TGWPICYTSSDSVFQVAAHELHFGLDRLYAICEQAKTIFDEANIARVIARPFTGSNGR
                     YRRTANRHDYTTPPPLPTLLDVLAAAGREVHGVGKIRDIFAARGISHHRPGDGNADLC
                     AQTIEAWRACPDGGLVFANLVDFDMLYGHRRDVAGYADALEAFDAFLPRLLAELRPGD
                     LLALSADHGCDPTWPGSDHTREHVPLLFAGPASPRGLDLGVRASFADLGQTLAAHLGV
                     PALAHGRACW"
     gene            complement(2929..>3542)
                     /locus_tag="VFA35_07350"
     CDS             complement(2929..>3542)
                     /locus_tag="VFA35_07350"
                     /inference="COORDINATES: protein motif:HMM:NF019448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HZT56023.1"
                     /translation="VLGTSCGNALELHEALAFLRGGPREPRLLALTRALSAELLLIGG
                     LADQADAALAQVDAALADGRALERFGRMVAALGGPADFVERADAYLPRAPVQRVLSAA
                     RSGWIATMATREIGLLVVELGGGRRQAGDAVDHRVGLSGVAAIGQRVEAGAPLATVHA
                     ADVASAAAACDKLAALIRIADAAPAVSPVLIERIAAVQTDHAP"
BASE COUNT          509 a         1225 c         1336 g          472 t
ORIGIN      
        1 ccacccgatg caccgacgtg cggcgcgtcg gtgtcgggcg caccctgggt cacgtacaac
       61 gagcacaggt tctcggagtc gacgtactcc tgcggaaaga ccgagaagct gcccgagtcg
      121 ttgaccggcg cgccgttgat cgtgaagccc atctggtcgc tgttgaagcc gcgcacccgc
      181 agattgccac cgaacaagcc ggtcgcatcg acgctggagc tgttcacgcc cggcagcagc
      241 gacaacgcct ggaacgggtt gccgctcgag cgggtctttt cgagctgggc cttggtgacc
      301 gagctgcgcg ccttgggcgc gtcttcgtcc atcagcaggc cggtgcccag cttgtcgccc
      361 tcgccggtga ccgtgacctt gttgagttcg gtggccgtct gcgcgtgcgc agcgccgacc
      421 atggcgagca gggccagggc caccgcgttg agcgtgaagg tacgcatggg gagggagtcc
      481 tttctgggga gcaagcggga gggaagacgg aaggagcgca gcgtgcccac gcgcacgtca
      541 acaaggccgc aaacgtttgc gcaaacttgt cggacggagg cggagaacgt gtgcatcggg
      601 agcgagatca gtgtggcagg cgaaggtgtg agtaaacgta gagcaaagac cttgccagcc
      661 cggggtgacg ccgtcgtgac atgttggtgc cgcacaacgg ctgagtgaat cgaaacagtg
      721 cctgagatgc cccgaaagcg ggctctctgc gagcttcagc gaacgaaaca ggcgcgaaac
      781 tcggcgcgca atcttggtgc atcaaccgcg acgcagacgg attgggccgg cgcgtcatcc
      841 cacgcggtag aggggaaacg ctggccctcc cgcacctgga tggtctgccc ggcagcgatg
      901 ccctcggtga ccacgcgcac cgggccgcgg cgcaacgtga aagctgttgc gtccaaggca
      961 caggcgacgg cgctggcgtc gtgcgagaag acgccgccgc cggtgcgcgg gcggtagaag
     1021 tcttcgtagt ggcgggtgat ctcgcggatg aacgcgccct cggcggcgcc ctcgcggccg
     1081 agcgcgtcga gccaggcggt gtcgagcagc acctcctggg tcacgtcgag gccgacgatc
     1141 gtgaccggcc agcgcgcggc gaaaacgcgg tcggctgcgt gcgggtcggc agcgatgttg
     1201 gcctcggcga ccggcgagac gttgccgcgg tggccgttct cgccgaacgc gccgcccatc
     1261 acgacgaccg ctttcacatg atcgaccagc gccgcgtcgt gcgccagcgc cagcgccagg
     1321 ttcgtcagcg ggccgaccgc gaccagcgtc agctcgcccg catggcgccg cgccagctcg
     1381 gcgatgaact gcggcgcggg ctggggcagc gcgcggcggg tgcgcgcggg cggcatgccg
     1441 atgtcgccca tcgcgtcgat gccgtgcacc tcggtcggga agccccccgg cggccgccgc
     1501 agcgcctgcg ccgcgccgcg cgcgaccggc gcctcgatcc cgaatcgctc gcacagcgcg
     1561 agcgcgttgg cggtcgtcag ctcgaccgcg gcattgccga acacggtcgt gatgccgagc
     1621 agctccgcgc gccggtcgcg ggcgagcatc agcagcgcca tcgcgtcgtc gatgccggga
     1681 tcggtgtcga acaggatctt cgtcgtcatc tcggccgggc gtctcaccag caggcgcggc
     1741 cgtgcgcgag cgcaggcacg ccgaggtgcg cggcgagcgt ctggcccaga tcggcaaagc
     1801 tggcacgcac gccgaggtcg agcccgcgcg ggctcgccgg gccggcgaac agcagcggca
     1861 cgtgctcgcg cgtgtggtcg ctgccgggcc aggtcgggtc gcagccgtgg tcggcgctga
     1921 gcgcgagcag gtcgccgggg cgcagctcgg cgagcaggcg cggcaggaag gcgtcgaagg
     1981 cttcgagcgc gtcggcgtag ccggcgacgt cgcggcgatg gccgtagagc atgtcgaagt
     2041 cgaccaggtt cgcgaacacc agcccgccgt cggggcaggc ccgccaggct tcgatcgtct
     2101 gcgcgcacag gtcggcattg ccgtcgccgg ggcggtggtg gctgatgccg cgcgcggcga
     2161 agatgtcgcg gatcttgccg acgccgtgga cctcgcggcc ggccgcggcc aacacgtcca
     2221 gcagtgtcgg cagcggcggc ggcgtggtgt agtcgtggcg gttcgcggtg cggcggtagc
     2281 ggccgttgct gccggtgaac ggccgcgcga tcacccgcgc gatgttggcc tcgtcgaaga
     2341 tcgtcttggc ctgctcgcag atcgcgtaca gccggtcgag gccgaagtgc agctcgtgcg
     2401 cggccacctg gaacacgctg tcgctgctcg tgtagcagat cggccagccg gtgcgcaggt
     2461 gttcgtcgcc gagccgctcg atgatctcgg tgccgctggc gtggcagttg ccgaggctgc
     2521 ccgcgagccc gcatgcggcg agccagcgct gcatcagcgc gggcgggaac accggcacgg
     2581 ccgggtcgcc ggaggtcgcg aagtaacccc agtcgaaggc gaccgggcag cccatcagct
     2641 cccagtggcc actgggcgtg tccttgccca ggctggtctc ggcggcggcg ccgtaggcgc
     2701 cttgtggtgc ggggtcgacg accacgcctt caggccagcg cccgctcgcc gcgtgcgcgg
     2761 cctgcggcag gccgagccgc gcgaggttcg gcagccgcag cggccggccg tgcgcggcgc
     2821 accactgcgc gatgtggccg aaggtgtcgg cgccgacatc gccgaagcgc ggcgcgtcgg
     2881 gcgcggcgcc gaggccgaac gagtcgagca cgatgacgag ggcgcgggtc atggcgcatg
     2941 gtccgtttga acagcggcga tgcgctcgat cagcaccggc gacacggccg gcgcggcatc
     3001 ggcgatgcgg atcagcgccg cgagcttgtc gcaggccgcg gcggcactcg cgacgtcggc
     3061 cgcgtgcacg gtcgcgagcg gcgcgccggc ctcgacgcgc tgcccgatcg cggccacgcc
     3121 gctcaggccg acgcggtgat cgaccgcgtc gcccgcctgc cgccggccgc cgccgagttc
     3181 gacgacgagc aggccgatct cgcgcgtcgc catcgtcgcg atccagccgc tgcgcgccgc
     3241 gctcagcacg cgctgcaccg gcgcccgcgg caggtacgcg tcggcgcgct cgacaaagtc
     3301 cgccggcccg ccgagcgccg cgaccatgcg cccgaagcgt tccagcgcgc ggccgtcggc
     3361 gagcgcggcg tcgacctgcg cgagcgcggc atccgcttgg tccgccagcc cgccgatcag
     3421 caacagttcg gcgctcagcg cccgcgtcag cgcgagcaac cgcggctcgc gcggcccgcc
     3481 gcgcaggaac gcaagcgcct cgtgcagttc gagcgcgttg ccgcagctcg tgccgagcac
     3541 tt
//
LOCUS       DASRDG010000440         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_992201, whole genome shotgun sequence.
ACCESSION   DASRDG010000440 DASRDG010000000
VERSION     DASRDG010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992201"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFA35_07355"
     CDS             complement(<1..189)
                     /locus_tag="VFA35_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56024.1"
                     /translation="MNAAAVGAAFARQAELCAQFAAPLYADLCRRAADEARAGGAEAA
                     DGAEIAGPIDDDGVARIDQ"
     gene            251..>2032
                     /locus_tag="VFA35_07360"
     CDS             251..>2032
                     /locus_tag="VFA35_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56025.1"
                     /translation="MLPSCAVLFASFALPAQAPPRDAMHVPPASGWEHCTPAAAGMDA
                     DRLAAAIAFAQGHATDWPADFSTQEQQFGRLLGPIPKTRADTNGVIVRHGCVVAEFGD
                     TAAVDPTYSVAKSMLSTVAAVAVREGRLGDLDEPVAARVHDGGYDGAQNAAVTWRHHL
                     QQESEWQGEMWGKNADFVGRDAFGQGERRPRALQPPGSHYEYNDVRINRLALSLLAVL
                     ERAVPDVFRDEVMAKVGASDGWRWIPYDNASVDLRGARVPSVSGGTRWGGGVWISSLD
                     LARFGLLWLRGGRWGERQILPADYVAAALRPSAHGPDYGFLWWLNSKQQNWPGLPANA
                     FGARGAGSNTVFCSPDHDLVVVWRWHSGGDHADARFFAKVIDAITGHRQPATQQPAAK
                     QPIAQVRTRDGELIEGALALPALAFAVDGAPRTVAVGELLSLQTAAAASDAENARIAG
                     GLAALAGADAKAAEAAAEELTDIGLPVLTPLLRSYADTDAHEPDLRYRLFARIVPGRA
                     DAHDRTLDLIRLADGAALRGRWTPTDLQLRLADGSLRTIPGASVRRLAVRRAQLERTF
                     ELQALHDCTYVGFLDTGVAVTGTTQLRA"
BASE COUNT          260 a          749 c          767 g          256 t
ORIGIN      
        1 ctggtcgatc cgggcgacgc cgtcatcatc gatcggcccg gctatctcgg cgccatcggc
       61 agcttccgcg ccgccggcgc gcgcctcgtc ggcggcgcgg cggcagagat cggcgtagag
      121 cggcgccgcg aactgcgcgc acagctcggc ctggcgcgcg aacgcggcgc cgacggcggc
      181 ggcgttcacg ctcgaatcat gctggcgcag tgcagcatcg gcggcgtgaa atgcaggaat
      241 gcttcgaccc gtgctgccgt cgtgcgccgt cctgttcgcc tcgttcgcgt tgcccgcgca
      301 agcgccgccg cgcgacgcga tgcacgtgcc gcccgcgagc ggctgggagc actgcacgcc
      361 ggcggccgct ggcatggatg cggacaggct cgccgccgcg atcgcgttcg cgcaggggca
      421 cgcgaccgac tggcccgccg acttcagtac gcaggagcag cagttcggca ggctgctcgg
      481 gccgatcccg aagacgcgcg ccgacaccaa cggcgtgatc gtgcggcacg gctgcgtggt
      541 cgccgagttc ggcgacaccg ccgccgtcga cccgacctac tcggtcgcga agagcatgct
      601 gtcgacggtg gcggcggtcg ccgtccgcga aggcaggctc ggcgatctcg acgagccggt
      661 cgccgcgcgc gtgcacgacg gcggctacga cggcgcgcag aacgcggcgg tcacctggcg
      721 ccaccacctg cagcaggagt cggagtggca gggcgagatg tggggcaaga acgccgactt
      781 cgtcggccgc gacgcgttcg ggcagggcga gcgccggccg cgcgcgctgc agccgcctgg
      841 cagccactac gagtacaacg acgtgcgcat caaccgcctc gcgctgtcgc tgctggctgt
      901 gcttgagcgc gcggtgcccg acgtgttccg cgacgaggtg atggcgaagg tcggcgcgtc
      961 ggacggctgg cggtggatcc cgtacgacaa cgcgtccgtc gacctgcgcg gtgcgcgcgt
     1021 gccgtcggtg agcggcggca cgcgctgggg cggcggcgtg tggatcagct cgctcgacct
     1081 cgcgcgcttc gggctgctgt ggctgcgcgg cggccgctgg ggcgaacggc agatcctgcc
     1141 tgcggactac gtcgccgcgg cgctgcggcc gagcgcgcac ggccccgact acggcttcct
     1201 gtggtggctc aacagcaagc agcagaactg gcccgggctg ccggcgaacg cgttcggcgc
     1261 gcgcggcgcc ggcagcaaca cggtgttctg ctcgcccgac cacgacctcg tggtcgtctg
     1321 gcgctggcac agcggcggcg atcacgccga cgcacggttc ttcgccaagg tgatcgacgc
     1381 gatcaccggg catcggcagc cggcgacgca gcagccggcc gcgaagcaac cgatcgcgca
     1441 ggtgcgcacg cgcgacggcg agctgatcga aggcgcgctc gcgctgccgg cgctcgcgtt
     1501 cgcggtcgac ggcgcgccgc gcacggtcgc cgtcggcgag ctgctgtcgc tgcagacggc
     1561 ggcggctgcg tccgacgccg agaacgcgcg catcgcgggc ggcctcgccg cgctcgcggg
     1621 cgccgacgcc aaggccgccg aggccgcggc cgaggaactg acggacatcg gcctgccggt
     1681 gctgacgccg ctgctgcgca gctacgccga caccgacgcg cacgagcccg acctgcgcta
     1741 ccgcctgttc gcgcgcatcg tcccgggccg cgccgacgcg cacgaccgca cgctcgacct
     1801 gatccgcctc gccgacggcg cggcgctgcg cggccgctgg acgccgacgg acctgcagct
     1861 gcggctcgcg gacggctcgc tgcgcacgat tccgggtgct tcggtgcgcc ggctcgcggt
     1921 gcggcgcgcg cagctcgagc gcacgttcga gctgcaggcg ctgcacgact gcacctacgt
     1981 cggcttcctc gacaccggcg tcgcggtcac cgggacgacg cagctgcgcg cc
//
LOCUS       DASRDG010000441         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_68011, whole genome shotgun sequence.
ACCESSION   DASRDG010000441 DASRDG010000000
VERSION     DASRDG010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68011"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1668
                     /gene="istA"
                     /locus_tag="VFA35_07365"
     CDS             127..1668
                     /gene="istA"
                     /locus_tag="VFA35_07365"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZT56026.1"
                     /translation="MERSAVHLLRKRGKSLRAIAAELGHSKTTIARALSEPVDQLPPR
                     RRRVSRVDPWRSQIAEWLRQGLSGVRMLELARDDAEQPFGGCDSVWRAAVRQERLVLE
                     HEQAVADVPIRFEGLPGEYLQVDWGEIRHFPFTQQSEGRRYFLACRLKYSRWVWVRFT
                     TDMRQETLFRGLVDCFNALGFVPWVLVFDNMKTVTTGRDDQEQPIWHRSLLQLAAEFD
                     FHPVACWPDSGNQKGSVESLVKFTKTNFLVGRTFVDDADLEAQCLQWQMRVNAERPSQ
                     ATDVTPLARLVEESAKGGTLPVTAGDYGFAEPGKVSRGAMVPILGNSYSVPVEHVGAP
                     VTVRVHRQRIVIWRDAQLLAEHRRAPDGGHRRVIDPAHFAPVFGRKPRARVMLYREVL
                     LELGDVARSYLSELSHRQRSRLGDEVLEVYALYERCGVGELLAAMELAHAQGAYGAAY
                     LRALVSAPEGEPASQVIELTLADLPSQTEIDRALSAYEIYVWTPDPRRPVSDELALVT
                     AGAQP"
     gene            1665..>2377
                     /gene="istB"
                     /locus_tag="VFA35_07370"
     CDS             1665..>2377
                     /gene="istB"
                     /locus_tag="VFA35_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZT56027.1"
                     /translation="MSVPSGSAGGLQPLLEHLGLHQAASVLAEWLDRAAVQDVSYADF
                     LQGLLEEESAARANAATQKRLRQAGFPYAATIEQFDFHFRPELKRQVVLRYLDPTFVE
                     QARSLALIGPPGLGKTMLAICIATKHIQLGATARFITAQHLANQLGRSSTSMGRQRLL
                     RPLLACDVLVLDELGYLPTLPGFGPALYELIAGRYERRPTLITSNKSLTEWATIVQDA
                     SLAAALVDRILHHGEVFYL"
BASE COUNT          385 a          768 c          814 g          410 t
ORIGIN      
        1 acaccgtaac ccgagaatcg gcggcctgac ccggagacgg ctttcacagc aaactggtac
       61 ggggggttgg cgtcgtgacg ctaccgtggc ccagccgagg agctgggaga aagggtgtta
      121 cgacgtatgg aacgcagtgc cgtccacttg ctgcgcaagc gtggcaagag tctgcgagcg
      181 atcgcggccg aactgggcca tagcaagacg accatcgccc gcgcgctgag cgagccggtt
      241 gatcagctgc cgccgcgccg ccggcgggtg tcgcgcgtcg atccgtggcg gagccagatc
      301 gccgagtggc tgcgccaggg cctgagtggc gtacgcatgc tggagttggc tcgggacgat
      361 gccgagcagc cgtttggcgg gtgtgattcg gtgtggcgcg cggcggtgcg gcaggagcgg
      421 ctggtcctcg agcacgagca ggccgtagct gacgtgccga tccgcttcga aggcttgccg
      481 ggtgagtact tgcaggtgga ttggggcgag attcgccact tcccgttcac gcagcagtcg
      541 gagggcaggc gctatttcct ggcgtgtcgg ctcaagtaca gccgctgggt gtgggtgcgc
      601 tttacgaccg atatgcgtca agagacgctg ttccgcggat tggtggattg cttcaacgcg
      661 ctgggcttcg tgccctgggt gctcgtgttc gacaacatga agacggtgac caccggccgt
      721 gatgatcagg agcagccgat ctggcaccgc tcgctgctgc agttggccgc ggagttcgat
      781 ttccacccag tggcgtgttg gccggactcg ggcaatcaga agggcagcgt cgaatccctg
      841 gtcaagttca cgaaaaccaa tttcctggtc ggccgcacat tcgtcgacga cgctgacctg
      901 gaggcgcagt gcctgcagtg gcagatgcgg gtcaatgccg agcgaccatc ccaggctact
      961 gacgtcaccc cgctggcgcg gctggtcgag gagagcgcca aaggcggcac gctgccggtc
     1021 actgctggtg actacggctt cgccgagcca ggcaaggtca gccgcggcgc catggtgccc
     1081 atcctcggca acagctactc ggtgcccgtc gaacacgtgg gtgccccggt gacggtgcgt
     1141 gtgcatcgcc agcgcatcgt catctggcgc gatgcgcagc tgctcgcaga gcaccggcgg
     1201 gcacccgacg gtggccaccg ccgcgtcatc gatccggctc acttcgcgcc cgtgttcggt
     1261 cgcaagccac gcgcccgagt catgctgtac cgcgaggtgc tgctcgagct cggcgacgtc
     1321 gcgcgcagct atctcagcga actcagccat cgccagcgca gccggttggg cgacgaagtg
     1381 ctcgaggtct acgcactcta cgaacggtgt ggcgtcggcg agctgctggc tgccatggag
     1441 ctcgcccacg cccagggtgc ttacggcgcg gcgtatctgc gcgcgctggt cagcgcgcct
     1501 gaaggcgagc cggccagcca ggtgatcgag ctgacgctgg ccgacctgcc gtcccagacc
     1561 gagatcgatc gcgcgctgag cgcgtacgag atctacgtgt ggacgcctga tccgcgccgc
     1621 cccgtcagcg acgagctggc cctggtgacc gctggagccc agccatgagc gtgccctctg
     1681 ggtccgccgg cggcttgcag ccactgctgg agcacctcgg cctgcaccag gcagccagcg
     1741 tcctggcgga gtggttggac cgtgccgccg tccaggacgt gagctacgcc gatttcttgc
     1801 agggcctgct cgaagaagaa agtgccgcgc gcgcaaacgc cgccacccag aagcggctgc
     1861 gccaggccgg ctttccgtac gccgcgacca tcgagcagtt cgatttccac tttcgaccgg
     1921 agctcaagcg tcaggtggtg ctgcgttacc tggaccccac ctttgtcgag caggcgcgct
     1981 cgctggcgtt gatcggacca cccggtctgg gcaagacgat gctggcgatc tgcattgcca
     2041 ccaagcacat ccaactgggt gccaccgcgc gcttcatcac cgcgcagcac ctggcgaacc
     2101 agctggggcg ctcctcgacc agcatgggcc gtcagcggct gctgcgaccg cttctagcgt
     2161 gcgacgtgtt ggtgctggat gagctggggt atctgccgac gttgcccggc ttcggtccgg
     2221 cgctgtacga gctgatcgcc ggtcgctacg aacgacgccc aaccctgatc acttccaaca
     2281 aaagcctgac ggaatgggcc accatcgtcc aggacgccag tttggcggcc gcgttggtcg
     2341 accgcattct gcaccacggc gaggtgttct acctcaa
//
LOCUS       DASRDG010000442         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1449797, whole genome shotgun sequence.
ACCESSION   DASRDG010000442 DASRDG010000000
VERSION     DASRDG010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449797"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..2185)
                     /gene="fusA"
                     /locus_tag="VFA35_07375"
     CDS             complement(119..2185)
                     /gene="fusA"
                     /locus_tag="VFA35_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZT56028.1"
                     /translation="MPSPPSGAGPGADVASIRTLALVGSAAAGKTSLAEALLATAGAI
                     GAPGTLDRGTTVSDFDAMERRMQHSLNASVLHLAHGATRIHFIDTPGAPDFLGQSLPA
                     LEAVETVAVVINAVTGVEPMAVRMMEHAASRQRDRLIIVNKIDAPGVDLPGVLQQIQA
                     AFGKECLPVNLPDADGSRVVDCFHNREGHSDFGSVDAVHRSLVEQVVEVDADFVDRYL
                     NEGDVDVSELHAPLEQALREGHLIPVCFVSARSGAGVAELLDIIVKLLPNPAEANPPD
                     FLRGEGDAAEPLAALPDPARHVLAHVFKVTVDPFVGRLGVFRVHQGTVTKDSLLYIGD
                     GRKPFKVGRLFMLQGKDHVEVAQALPGDIAAVAKVDEIRFDAVLHDAAEDDHIHLAPL
                     PFPRPVHGLAIEPKRHGDEQRMWEILTKLVDEDPCLKLEHVTGTNETVVYGLGELHLR
                     ILLERLRETYRFEVNTRPPRVAYRETVTAPAEGHHRHKKQSGGAGQFGEVFLRIEPLP
                     RGTGFEFLDQVKGGTIPGQFIPAVEKGVREVLAAGAIAGYPVVDVRVVVYDGKHHSVD
                     SKEIAFVTAGRKAFLAAVRDGRPIVLEPIVHVEITAPGQALGDITGDLASRRGMVSGT
                     ANGAPGTMMVRGQAPMSELLGYQSRLNALTSGQGRYTVEFSHYEAVPPNTQQRLVGEF
                     KVRDDD"
BASE COUNT          345 a          832 c          856 g          396 t
ORIGIN      
        1 atcgtcacct ccaaaacgaa cgatcgtttc gaccccacac agggcgcgcg ggccctccgc
       61 ccaatggtcg ggcgcggccg tcgcttcgaa acccgagcgc tcgagctcag gaaggttttc
      121 agtcgtcgtc gcgcaccttg aactcgccga cgagccgctg ctgcgtattc ggcggcaccg
      181 cctcgtagtg cgagaactcg accgtgtacc ggccctggcc gctggtcagc gcgttgagcc
      241 ggctctggta gccgagcagc tccgacatcg gcgcttggcc gcgcaccatc atcgtcccgg
      301 gcgcgccgtt ggccgtgccg ctcaccatgc cgcggcgcga cgccaggtcg ccggtgatgt
      361 cgccgagcgc ctgcccgggc gccgtgatct cgacatgcac gatcggctcg agcacgatcg
      421 gccgaccgtc gcgcaccgcc gccaggaagg ccttgcggcc ggcggtgacg aaagcgatct
      481 ccttgctgtc gacgctgtgg tgcttgccgt cgtagaccac cacccgcacg tcgaccaccg
      541 gatagccggc gatggcgccg gcggcgagca cttcgcgcac gcctttttcc acggcgggga
      601 tgaactggcc cggaatcgtg ccgcccttga cctggtcgag gaactcgaag cctgttccgc
      661 gcggcagcgg ctcgatgcgc aggaagacct cgccgaactg gccggcgcca ccgctctgct
      721 tcttgtgccg atggtggcct tccgccggcg ccgtgacagt ttcgcggtag gcgacccgcg
      781 gcggccgcgt gttcacttcg aagcggtagg tctcgcgcag ccgctcgagc aggatgcgca
      841 ggtgcagctc gccgaggccg tagaccacgg tctcgttggt gccggtcacg tgctccagct
      901 tcaggcaggg atcttcatcg accagcttgg tcaggatctc ccacatccgc tgttcgtcgc
      961 cgtggcgctt gggctcgatc gccaggccgt gcaccggcct gggaaagggc aacggcgcga
     1021 ggtggatgtg atcgtcctcg gcggcgtcgt ggaggaccgc gtcgaagcgg atctcgtcga
     1081 ccttggcgac cgcggcgatg tcgcccggca gcgcctgcgc gacttcgaca tggtccttgc
     1141 cctggagcat gaagagccgc ccgaccttga agggcttcct gccgtcgccg atgtacagca
     1201 ggctgtcctt ggtgaccgtc ccctggtgaa cgcggaacac gccgagccgg ccgacgaagg
     1261 gatcgaccgt caccttgaac acatgggcca ggacgtgccg ggccgggtcg ggcagcgcgg
     1321 cgagcggctc ggccgcgtca ccctcgccgc gcaggaagtc cggcgggttg gcctcggcgg
     1381 gattcggcag cagcttgacg atgatgtcga gcagctcggc cacgccggcg ccgcttcggg
     1441 ccgagacgaa gcacaccggg atcaggtggc cctcgcgcag cgcctgctcc agcggcgcgt
     1501 gcagctccga gacatcgacg tcgccctcgt tgagatagcg gtcgacgaaa tcggcgtcca
     1561 cctcgacgac ctgctcgacc agcgaccggt gcacggcatc gacgctgccg aaatcgctgt
     1621 gcccttcacg gttgtggaag cagtcgacga cccggctgcc gtcagcatcg ggcaggttga
     1681 ccgggaggca ctccttgccg aacgcggcct ggatctgctg cagcacgccg ggcaggtcga
     1741 cgcccggggc gtcgatcttg ttgacgatga tcaggcggtc gcgctggcgc gaggcggcgt
     1801 gctccatcat gcgcaccgcc atcggctcga cgccggtcac cgcgttgatg accaccgcca
     1861 cggtctccac cgcttcgagc gcgggcaggc tctgcccgag gaagtcggga gcgcccggcg
     1921 tgtcgatgaa gtggattcgc gtcgcgccgt gcgcaaggtg gagcacgctg gcattgagcg
     1981 aatgctgcat tcgccgctcc atcgcgtcga agtcgctgac cgtggtgcca cggtccagcg
     2041 ttccgggggc gccaatcgcg ccggcggtcg cgagcaaggc ctcggccagg ctggtcttgc
     2101 cggcagccgc cgatccgacc agcgccagcg tgcgtatcga tgcgacgtcg gcgccggggc
     2161 cggcgccaga tggtgggctt ggcatgcggg tgtctccttc ggcgggcgcc ggtgcgtggc
     2221 cggcggcggt ggatccaggt gcggtgtcga tgatcctata cgtacgggct gagggtgtca
     2281 ggcacatccc ggggctcgcg gcggcctcag ggcttctcgc gcgcgccgtg ctgctcgagg
     2341 taggtcatca gcgcgacgtc gcgcgcttcg atggcagcca cgagcggcgt gatgctcggg
     2401 tggcaattca cgtccacgcc gccgtcgat
//
LOCUS       DASRDG010000443         4486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_992597, whole genome shotgun sequence.
ACCESSION   DASRDG010000443 DASRDG010000000
VERSION     DASRDG010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992597"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFA35_07380"
     CDS             complement(<1..354)
                     /locus_tag="VFA35_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZT56029.1"
                     /translation="MSSPPADPTGGPPAAAPADAALPRGLRLGGFEIQKVLARSASTI
                     VYLATDHALAMPVALQEYLPARLGVRDAGLHLRPTEPRHEDVIARGRRAFIDEARMLA
                     HCDHAALVRVTQLFEA"
     gene            527..1270
                     /locus_tag="VFA35_07385"
     CDS             527..1270
                     /locus_tag="VFA35_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56030.1"
                     /translation="MRDFEDSTLWRISEFERVRSRSGGSAFARLGGASVLPTTLLADL
                     RRLDASGASDEPLEVVAACIRHREAALLCLQYDGLVWPVTLFPQQMLYHSPRDLTQAL
                     PTALAHITLISAEPPGVRPPGHWMHERIAQVEHYRPLLPLLWSLALDGPRATLLAEIA
                     GTAAYRALKNPEADGATLRGPLRSAAQRLRRETVALRDIAAWPGMGVDRASRLLNGFY
                     LSSSLLATRSHPAARAEPRGLWRGAKLWR"
     gene            complement(1281..2252)
                     /locus_tag="VFA35_07390"
     CDS             complement(1281..2252)
                     /locus_tag="VFA35_07390"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HZT56031.1"
                     /translation="MTTPVLEAQALAKHYPVSQGLFKPKALVRALDSVSFALEAGQTL
                     AVVGESGCGKSTLARQLTMIEPATSGRLLFAGADVIHADAALKKQLRRQVQMVFQNPF
                     ASLNPRKKIGHALEEPLVINTDATKAARTERARAMMARVGLRPEHDARYPHMFSGGQR
                     QRIAIARALMLEPRIVVLDEPVSALDVSVQAQVLNLLMDLQDETRVAYVFISHNLAVV
                     ELIADEVLVMYLGKVVEQAPKATLFATPRHPYTRALLASTPRIDKAARQQRQVLSGEL
                     PSPLNPPSGCAFHKRCPFALERCRVEVPMLEDLGGGQRVACHRVHEI"
     gene            complement(2249..3211)
                     /locus_tag="VFA35_07395"
     CDS             complement(2249..3211)
                     /locus_tag="VFA35_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZT56032.1"
                     /translation="MSALLEVENLGVSFGAFRAVDGVSLSVGPGEVVGIVGESGSGKS
                     VTALALMGLVDEPGVVAATRLDFDGRDLLTMREHDRRQMLGRDIAMIFQDPMTSLNPC
                     FTVAFQLIETLRIHEGGSNKALRARALQLLRDVEIPDAARRLDAYPHQLSGGMSQRVM
                     VAMAIACNPRLLIADEPTTALDVTIQAQMLALLLKLQRERGMALVLITHDLGVVAEVA
                     QRVLVMYAGQVVETAAVPQIFEAPRHPYTEALLSALPEHNIGRRRLRSMGGIVPGAFD
                     RPAGCLLAPRCPYVQERCRVEKPALAGAPGAQARCFFPLGEGAP"
     gene            complement(3208..4092)
                     /locus_tag="VFA35_07400"
     CDS             complement(3208..4092)
                     /locus_tag="VFA35_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZT56033.1"
                     /translation="MSAVLNQSPPSPFAELWRDFRRNRGAMVGLAVIVALLLCALLAD
                     VIAPYSPKEQFTDAVLKPPSMQPIGGHVFLLGTDPVGRDLLSRLIHGTRLSLLIGLVS
                     VSLSLSIGALLGLLAGFFRGGVEFTIMRLMDIMLALPSLLLAVAVVAILGPGFNNAMV
                     AIAIVMLPHFVRLTRAAVIGELAKDYVNASRIAGAGTARLMFDTVLPNCAAPLIVQAT
                     LGFSTAILDAAALGFLGLGAQPPTPEWGSMLASALEFIQSAWWVVTLPGLAILVSVLA
                     FNLMGDGLRDALDPKLKR"
BASE COUNT          646 a         1580 c         1628 g          632 t
ORIGIN      
        1 cgcctcgaac agttgcgtca cgcgcaccag cgccgcgtga tcgcaatgcg cgagcatgcg
       61 ggcctcgtcg atgaaggcac gccggccgcg cgcgatcacg tcttcgtggc ggggctcggt
      121 cgggcgcagg tgcaggccgg catcgcgcac gccgaggcgg gccggcaggt attcctgcag
      181 cgcgaccggc atcgccagcg cgtggtcggt ggcgaggtag acgatcgtgc tggcgctgcg
      241 ggcgagcacc ttctggatct cgaaaccgcc gagccgcaga ccgcgcggca aggccgcgtc
      301 ggccggcgcc gccgcgggcg ggccgcccgt ggggtcggcc ggcggtgagc tcaatcggtc
      361 gttcattcgg gcaggtgtcg ggcagatctc gagcagaggg cagcgaagta ttgcaagctt
      421 agcctgccgg tgccgcgcca cggcggcatt tgcagctcgt gacagttgct tgcaggccgt
      481 acatccggcc acgtctggct cgcgcgcagg ccgcgaacat gggcccatgc gggacttcga
      541 ggactccacc ctgtggcgca tcagcgagtt cgagcgcgtg cgctcgcgca gcggcggtag
      601 cgcgttcgcg cggctcggcg gcgcgagcgt gctgccgacc acgctgctcg ccgacctgcg
      661 ccggctcgac gcctccggcg ccagcgacga accgctggag gtcgtggcgg cctgcatccg
      721 ccaccgcgag gcggcgctgc tgtgcctgca gtatgacggg ctggtctggc cggtcacgct
      781 gttcccgcag cagatgctgt accactcgcc gcgcgacctg acgcaggcgc tgccgaccgc
      841 gctggcgcac atcacgctga tcagcgccga gccgcccggc gtgcgcccgc cggggcactg
      901 gatgcacgag cgcatcgcgc aggtcgagca ctaccggccg ctgctgccgc tgctgtggtc
      961 gctcgcgctc gacggcccgc gcgcgacgct gctggccgag atcgcgggca ccgcggccta
     1021 ccgcgcgctg aagaaccccg aggccgacgg cgcgaccctg cgcggcccgc tgcgctcggc
     1081 cgcgcagcgc ctgcggcgcg agacggtcgc gctgcgcgac atcgccgcct ggccggggat
     1141 gggcgtcgat cgtgcgagcc ggctgctcaa cggtttctac ctgtcgtcgt cgctgctcgc
     1201 gacgcgctcg cacccggcgg cgcgcgccga gccgcgcggg ttgtggcgcg gcgccaagtt
     1261 gtggcgttga tcgcttttcg ctagatctcg tgcacccggt ggcaggccac gcgctgcccg
     1321 ccgcccaggt cttcgagcat cggcacctcg acccggcagc gttcgagcgc gaacgggcag
     1381 cgcttgtgga acgcgcagcc gctcggcggg ttcagcggtg acggcagctc gccgctgagc
     1441 acctggcgct gctggcgcgc ggccttgtcg atgcgcggcg tgctcgcgag cagcgcgcgc
     1501 gtgtacgggt gacgcggcgt cgcgaacagt gtggccttcg gcgcctgttc gacgaccttg
     1561 ccgaggtaca tcaccagcac ctcgtcggcg atcagttcga cgaccgcgag gttgtgcgag
     1621 atgaacacgt aggcgacgcg ggtctcgtcc tgcaggtcca tcagcaggtt cagtacctgc
     1681 gcctggaccg agacatcgag cgccgagacc ggctcgtcga ggacgacgat cctgggttcg
     1741 agcatcagcg cgcgcgcgat cgcgatgcgc tggcgctggc cgcccgagaa catgtgcggg
     1801 tagcgcgcat cgtgctccgg ccgcagcccg acgcgcgcca tcatcgcgcg cgcgcgctcg
     1861 gtgcgcgcgg ccttggtcgc atcggtgttg atgacgagcg gctcttcgag cgcgtggccg
     1921 atcttcttgc gcgggttcag gctcgcgaac gggttctgga acaccatctg cacctgccgg
     1981 cgcagctgct tcttcaacgc cgcatcggcg tggatcacgt cggcgccggc gaacagcagc
     2041 cggcccgacg tggccggctc gatcatcgtc agctgccgcg ccagcgtgct tttgccgcag
     2101 cccgattcgc cgacgacggc gagcgtctgg cccgcttcga gcgcgaacga gacgctgtcg
     2161 agcgcacgca ccagcgcctt cggcttgaac aggccttgcg aaaccgggta gtgcttggcg
     2221 agggcctggg cttcgagaac gggggtggtc atggcgcgcc ctcacccagc ggaaagaagc
     2281 agcgcgcctg cgcgcccggc gcgcccgcca gcgccggctt ctcgacgcgg cagcgttcct
     2341 gtacataggg gcagcgcggc gcgagcaggc agccggcggg ccggtcgaac gcgcccggca
     2401 cgatgccgcc catcgagcgc aggcggcgcc ggccgatgtt gtgctcgggc agcgccgaga
     2461 gcagcgcctc ggtgtaaggg tgccgcggcg cctcgaagat ctgcggcacc gccgcggtct
     2521 cgacgacctg gccggcatac atcaccagca cgcgctgcgc cacctcggcg acgacgccga
     2581 ggtcgtgcgt gatcagcacc agcgccatgc cgcgttcgcg ctgcagcttc agcagcagcg
     2641 cgagcatctg cgcctggatc gtcacgtcga gcgcggtggt cggctcgtcg gcgatcagca
     2701 ggcgcgggtt gcaggcgatc gccatcgcga ccatcacgcg ctggctcatg ccgccgctga
     2761 gctggtgcgg gtaggcatcg agccggcgcg cggcgtcggg gatctcgacg tcgcgcagca
     2821 gctgcagcgc gcgtgcgcgc aaggccttgt tcgagccgcc ttcgtggatg cgcagcgtct
     2881 cgatcagctg gaacgcgacc gtgaagcacg ggttcaggct cgtcatcggg tcctggaaga
     2941 tcatcgcgat gtcgcggccg agcatctggc ggcgatcgtg ctcgcgcatc gtcagcaggt
     3001 cgcggccgtc gaagtcgagg cgcgtcgccg cgacgacgcc gggctcgtcg accaacccca
     3061 tcagtgccag cgcggtcacg ctcttgcccg agcccgattc gccgacgatg ccgacgacct
     3121 cgccgggtcc gacggacagg ctcacgccgt cgacggcgcg gaatgcgccg aacgagacgc
     3181 cgaggttctc gacttcgagc agcgcgctca tcgcttcagc ttcgggtcga gcgcgtcgcg
     3241 caggccgtcg cccatcaggt tgaacgcgag caccgagacc aggatcgcca ggcccggcag
     3301 cgtcacgacc caccacgcgc tctggatgaa ctcgagcgcg ctcgccagca tcgagcccca
     3361 ctcgggcgtg ggcggctgcg cgcccaggcc gagaaagccc aaagcggcgg cgtcgaggat
     3421 cgcggtcgag aagcccagcg tggcctgcac gatcagcggt gccgcgcagt tcggcagcac
     3481 ggtgtcgaac atcagccgcg cggtgccggc gccggcgatg cgcgaggcgt tgacgtagtc
     3541 cttcgccagc tcgccgatca ccgcggcgcg cgtcagccgg acgaagtgcg gcagcatcac
     3601 gatcgcgatc gcgaccatcg cgttgttgaa gcccggcccg aggatcgcga ccactgcgac
     3661 cgcgagcagc aggctcggca gcgcgagcat gatgtccatc agccgcatga tcgtgaactc
     3721 gacgccgccg cggaagaagc cggcgagcag ccccagcagc gcaccgatgc tcagcgacag
     3781 gctcaccgag acgagcccga tcagcagcga cagccgcgtg ccgtggatca gccgcgagag
     3841 caggtcgcgg ccgaccgggt cggtgccgag caggaacacg tggccgccga tcggctgcat
     3901 cgacggcggc ttcagcacgg cgtcggtgaa ctgctccttc ggcgaatacg gcgcgatcac
     3961 gtcggcgagc agcgcgcaca gcagcagcgc gacgatcacc gcgagcccga ccatcgcacc
     4021 gcgattgcgg cggaagtcgc gccagagctc ggcgaacggg gaggggggtg attggttgag
     4081 gacggcgctc atggcgttcg gcgggttggc gcgtcgcggc aggcggcacc cggcaggggc
     4141 tcatacggtg gcggtcgatg ctgcgcatcg actgcgttcc ggtgctcggt ccgaggtcgc
     4201 gccgcagaac tcgctacgct cactgcgttc gctgcgctcg gacaaccgcg gcgagtcaga
     4261 tcacgaagcg cgctgcgcgc gccgaccccg gccctgcgct tctcgtcgcc acagaaatcg
     4321 cccctgccgg gtaccccctg ccgcgagttc caccactgag cttttcgctc gacaagaacc
     4381 tcggtggttc ggcaaaggcg cgggcgggca gccggcggcg cgcctctgcg cagccgagaa
     4441 gcgcagggct cgtggcccgc gcgcgcagcg cgcttcgtga actgac
//
LOCUS       DASRDG010000444         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_298537, whole genome shotgun sequence.
ACCESSION   DASRDG010000444 DASRDG010000000
VERSION     DASRDG010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298537"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFA35_07405"
     CDS             <1..583
                     /locus_tag="VFA35_07405"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HZT56034.1"
                     /translation="VKRARTAFDRIAPAAADLMLTITPEQIRHLEQRYAKFNAEFRDN
                     YLQPEPRQRAAANLRRALDRAETLYGRFDDAQRARLAEALARSPFDAELWFAERRERQ
                     LEMLQMLRQAGAATAGPEQPLAALRGYAGRLERSPREPYRRYGERLKDFNCALAASLH
                     NATTAAQRRHAAQKLAGWESDLRALAATADEDN"
     gene            complement(601..1212)
                     /locus_tag="VFA35_07410"
     CDS             complement(601..1212)
                     /locus_tag="VFA35_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZT56035.1"
                     /translation="MFRLISALAMALVLAGCAGLNNLTNEVSTFGPWPAQRKPAGFVF
                     ERLPSQQQHPQRQTQLETAARGALETAGFRAAADADHAEYLMQIGARVTSNDPWIYNE
                     PLFWRGSFRYGHDRWGRGWGPWGPGWGFGGGIGYTPTFEREVALLIRDRKTGQLLYEA
                     RASNTGPGTAIDYMMPAMFEAALKDFPNAGPNPRSITTQISKQ"
     gene            complement(1200..2141)
                     /gene="ttcA"
                     /locus_tag="VFA35_07415"
     CDS             complement(1200..2141)
                     /gene="ttcA"
                     /locus_tag="VFA35_07415"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HZT56036.1"
                     /translation="MSAVLDNPAAKDKLSVELTKLGKRLHRQVGQAIVDFNMIEEGDK
                     VMVCVSGGKDSYALLDILIKLRARAPISFDIVAVNLDQKQPGFPEHVLPEYLKGLGIP
                     FHIENQDTYSIVKRVIPEGKTMCSLCSRLRRGILYRVAGELGATKIALGHHRDDILQT
                     LFMNMFFGAKLKGMPAKLVSDDGKNIVIRPLAYVSELDLERWAAHRAFPIIPCTLCGN
                     QENLQRVQTKQMLRDWDRRFPGRVDNLFNAMANVVPSHLMDRNLYPFATLQADGMADA
                     AGDRAFDDDGACGTPAPSATIPIRAQRRRAEETPCSD"
     gene            complement(2138..2539)
                     /locus_tag="VFA35_07420"
     CDS             complement(2138..2539)
                     /locus_tag="VFA35_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZT56037.1"
                     /translation="MHSLLSHPSLMDCRRLFLRDYEVFINIGVHAFEKTREQRVLINV
                     DLYVPLALSTPKADELVEVVDYDFMRRSIAERVGEGHIHLQETLCDDVLGRMLAHPKV
                     RAARVSTEKPDVYPDCHSVGCEVFGMKEKLA"
     gene            complement(2549..>3085)
                     /locus_tag="VFA35_07425"
     CDS             complement(2549..>3085)
                     /locus_tag="VFA35_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZT56038.1"
                     /translation="AIVNSASIFEYDDPAGFTVAAMEKHWRANTAPAILLAQALHAQL
                     DGCAGERAERTGCVVNLLDQKLWNPNPDYFSYTLSKAALQCATVMLAQALAPRVRVCG
                     VAPGVTLVSGPMNAAEFAQAHAMTPLARSSTPEDIAHAVRFLIESPAITGTTLLVDGG
                     QHLAVQPRDVLYLARQNH"
BASE COUNT          465 a         1031 c         1087 g          502 t
ORIGIN      
        1 cgtgaagcgc gcgcgcaccg cattcgaccg gatcgcgccc gccgccgccg acctgatgct
       61 gacgatcacg cccgaacaga tcaggcacct tgagcagcgc tacgcgaagt tcaacgccga
      121 gtttcgcgac aactacctgc agcccgagcc gcgccagcgc gccgcagcga acctgcggcg
      181 cgcgctcgac cgcgccgaga cgctttacgg gcgtttcgac gacgcgcagc gcgcccgcct
      241 ggccgaggcg ctggcgcgct cccccttcga tgccgagctg tggttcgccg agcgccgcga
      301 gcgccagctc gagatgctgc agatgctgcg ccaggccggc gccgcgacgg cgggccccga
      361 gcagccgctg gctgcgctgc gcggctacgc cggccggcta gagcgctcgc cgcgcgagcc
      421 gtaccggcgc tacggcgagc gcctgaagga tttcaattgc gcgctcgccg ccagcctgca
      481 caacgcgacc accgccgcgc agcgccgcca cgcggcgcag aagctggcgg gctgggagag
      541 cgacctgcgc gccctcgcgg cgaccgccga cgaagacaac tgatcgctgc gaggtcgagg
      601 ctattgcttc gagatctgcg tcgtgatgct gcgcgggttc ggcccggcgt tcgggaagtc
      661 tttcagcgcg gcctcgaaca tcgccggcat catgtagtcg atcgcagtgc cgggcccggt
      721 gttgctggcg cgcgcctcgt acagcaactg accggtcttg cgatcgcgga tcagcaacgc
      781 cacctcgcgc tcgaaggtcg gcgtgtaccc gatgccgccg ccgaaacccc agcccggacc
      841 ccacgggccc cagccacgcc cccagcggtc gtggccgtag cggaagctgc cgcgccagaa
      901 cagcggctcg ttgtagatcc acgggtcgtt gctggtgacg cgcgcgccga tctgcatcag
      961 gtattccgca tggtcggcat cggccgctgc gcgaaagccc gcggtttcga gggcgccgcg
     1021 cgctgcggtc tcgagctggg tctggcgctg cgggtgttgc tgctgcgacg gcaggcgctc
     1081 gaacacgaag ccggccggct tgcgctgcgc cggccagggc ccgaaggtcg agacctcgtt
     1141 ggtcaggttg ttgaggccgg cgcagccggc gagcaccagg gccatcgcaa gcgcggaaat
     1201 cagtctgaac atggtgtctc ctcggcgcgc cggcgctgcg cgcgaatcgg gatcgtggcc
     1261 gagggcgcag gtgtcccgca agcgccgtcg tcatcgaagg cgcggtcccc cgcggcatcg
     1321 gccatgccgt ccgcttggag tgtcgcgaac gggtacaggt tccggtccat caggtgcgat
     1381 ggcacgacgt tcgccatcgc gttgaacagg ttgtcgaccc ggccgggaaa gcgccggtcc
     1441 cagtcgcgca gcatctgctt ggtctgcacc cgttgcaggt tctcctggtt gccgcacagc
     1501 gtgcacggga tgatcggaaa cgcgcggtgc gcggcccagc gctcgaggtc gagctcgctc
     1561 acgtaggcga gcgggcggat cacgatgttc ttgccgtcgt cgctgacgag cttggccggc
     1621 atgcctttca gcttggcgcc gaagaacatg ttcatgaaca gcgtctgcaa tatgtcgtca
     1681 cggtggtgac cgagcgcaat cttggtcgca ccgagttcgc ccgcaactct gtaaagaatg
     1741 ccgcgccgca gccgcgagca caggctgcac atggtcttgc cctcggggat cacgcgcttg
     1801 acgatcgagt aggtgtcctg gttctcgatg tggaacggga tgcccaggcc cttcaggtac
     1861 tcgggcagca catgctccgg aaagcccggc tgcttctggt cgaggttgac cgcgacgatg
     1921 tcgaacgaga tcggcgcccg cgcccgcagc ttgatcagga tgtcgagcag cgcgtagctg
     1981 tccttgccgc ccgagacgca gaccatgacc ttgtcgccct cttcgatcat gttgaagtcg
     2041 acgatcgcct ggccgacctg gcggtgcagg cgcttgccga gcttggtcag ttcgaccgag
     2101 agcttgtctt tggcggctgg gttgtcgagc acggcactca tgcgagcttc tccttcattc
     2161 cgaacacctc gcagccgacc gagtggcaat cggggtagac atcgggcttc tcggtcgaga
     2221 cgcgcgcggc gcgcaccttg gggtgcgcga gcatgcgccc gagcacgtcg tcgcagagcg
     2281 tttcctgcag gtggatgtgg ccctcgccga cgcgctcggc gatgctgcgg cgcatgaagt
     2341 cgtagtcgac cacctcgacg agttcgtcgg ccttcggcgt cgagagcgcg agcgggacgt
     2401 acaggtcgac gttgatcagc acgcgctgtt cgcgcgtctt ctcgaacgcg tgcacgccga
     2461 tgttgatgaa cacctcgtag tcgcgcaaga agaggcgccg gcaatccatc aggctggggt
     2521 ggctgagcag gctgtgcatc ggagggtctc agtggttctg gcgggcgagg tagagcacgt
     2581 cgcgcggctg cacggccagg tgctggccgc cgtcgacgag cagcgtcgtg ccggtgatcg
     2641 ccggcgactc gatcaggaag cgcacggcgt gcgcgatgtc ctcgggggtc gacgagcgcg
     2701 cgagcggggt catcgcgtgc gcttgcgcga actcggcggc gttcatcggg cccgagacca
     2761 gcgtcacgcc cggcgccacg ccgcacacgc gcacgcgcgg cgcgagcgcc tgcgcgagca
     2821 tcacggtggc gcattgcagc gcggccttcg acagcgtgta cgagaagtag tcggggttcg
     2881 ggttccagag cttctggtcg agcaggttga cgacgcagcc cgtgcgctcg gcccgttccc
     2941 cggcgcagcc gtcgagctgc gcatgcaagg cctgcgcgag caggatggcc ggcgcggtgt
     3001 tggcgcgcca gtgcttctcc atcgccgcga ccgtgaagcc ggccgggtcg tcgtactcga
     3061 agatcgaggc gctgttgacg atcgc
//
LOCUS       DASRDG010000445         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_68515, whole genome shotgun sequence.
ACCESSION   DASRDG010000445 DASRDG010000000
VERSION     DASRDG010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68515"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VFA35_07430"
     CDS             complement(<1..1439)
                     /locus_tag="VFA35_07430"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZT56039.1"
                     /translation="MELDIVALSRLQFALTALYHFLFVPLTIGLSLLLAIMETVYVMT
                     GRPIWRDMTKFWGTLFGINFAMGVATGIVMEFEFGMNWSYYSHYVGDVFGAPLAMEGL
                     MAFFMEATFVGLFFFGWDKLSKVKHLIVTWLVALGTNFSALWILIANGWMQNPVGAKF
                     NPQTMRMEVTDFFAVLTNTVAQAKFVHTVSAGYVAASFFVLGVSAWYLVRGRHIQLAK
                     RSMTVAASFGLASAFSVVLLGDESGYLSTEHQKMKLASIEAMWHTEPAPAAFTAFGFP
                     DQEARETHYAVHIPWVMGLIGTRSLDTEIPGIVDLVKRAEGHIREGIKAYDALQQIRE
                     AKTDAEIPVQARYDFEINGHWLGYGLLLKRYVDDPRQATPEQIAKAAWDTVPQVAPLY
                     FGFRIMAGLGMFFILLMVAFFWLSARRRLDAHPWLLRVAVWAIPLPWIAIEAGWLVAE
                     FGRQPWIIEGVLPTAVAVSNLGVATLLITL"
     gene            complement(1485..3212)
                     /locus_tag="VFA35_07435"
     CDS             complement(1485..3212)
                     /locus_tag="VFA35_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT56040.1"
                     /translation="MSALVRPASGLRAVLRLFAAAQGRPLALGLLLAVTTVLMGIGLL
                     GLSGWFITASALAGAQVAIALAFDVFMPSAGIRLLALGRTLSRYGERLVTHDATLGVL
                     AALRERLFRAWSGPHAARQLQLRPARLLFRLTADIDALEALYLRLLVPAAATLGAALL
                     AGLIVGVQDARQGLALFAWLLAVGWGSAWLLARQAAQAAARRGAALEALRARTADLVA
                     GRTELAMAGRLEAQQRAIEAADARIAASDRRLNRLEVRSLFGHGAAAALALAGVLLGA
                     AWLVEQGRIGAPVAALMLLVALGAGEPLGALRRGALEAGRALLAARRVAPALELPDEA
                     PLLAQGRARAADGSMAPAVRLDGVRCAHAGSLGDVLVVPALAIAPGERLALVGASGAG
                     KSTLLALVAGELAPRAGRVEALAAAWLTQRIDLFQDSLRDNLRLADPAATDDELMAAL
                     DAAGLGADVRGLPRGLDTPLGEGGQGLSGGQARRLALARLLLQRRPLWLLDECTEALD
                     AATARDVLRRLDATADTGRRTLLIATHLRREAALADRLLQVEHGRIVRDLRRGTPGFD
                     TMLASLRPD"
BASE COUNT          526 a         1199 c         1148 g          390 t
ORIGIN      
        1 agcgtgatca gcagcgtcgc cacgccgagg ttcgacacgg ccaccgcggt cggcagcacg
       61 ccctcgatga tccagggctg ccggccgaac tcggccacga gccagccggc ttcgatcgcg
      121 atccacggca gcgggatcgc ccacaccgcg accctgagca gccacgggtg cgcgtcgagc
      181 cggcgccggg cggagagcca gaagaacgcg accatcagca ggatgaagaa catccccagg
      241 ccggccatga tgcggaagcc gaagtacagc ggcgccacct gcggcacggt gtcccaggcg
      301 gccttggcga tctgctcggg cgtggcctgg cgcgggtcgt ccacatagcg cttgagcagc
      361 aacccgtagc ccagccagtg gccgttgatc tcgaagtcgt agcgcgcctg caccgggatc
      421 tccgcatccg tcttcgcctc gcggatctgc tggagtgcgt cgtaggcctt gatgccttcg
      481 cggatgtggc cttcagcccg cttgaccagg tcgacgatgc ccggaatctc ggtgtccagc
      541 gagcgcgtgc cgatcaggcc catcacccag gggatgtgca ccgcgtagtg cgtctcgcgc
      601 gcctcctggt cggggaagcc gaaggcggtg aaggcggccg gcgcgggctc ggtatgccac
      661 atcgcctcga tcgaggcgag cttcatcttc tgatgctcgg tcgagaggta gccgctctcg
      721 tcgcccagca gcacgaccga gaacgccgag gccagcccga aggaagccgc gaccgtcatc
      781 gagcgcttgg ccagctggat gtgccggccc ctgaccaggt accacgcgga cacgcccagc
      841 acgaagaacg aggccgccac gtagccggcc gagacggtgt gcacgaactt ggcctgcgcg
      901 accgtgttgg tgagcacggc gaagaagtcc gtgacctcca tgcgcatggt ctgcgggttg
      961 aacttcgcgc ccaccgggtt ctgcatccag ccgttggcga tcaggatcca cagcgccgag
     1021 aagttggtgc cgagcgccac cagccaggtg acgatcaggt gcttgacctt ggagagcttg
     1081 tcccagccga agaagaacag cccgacgaag gtcgcttcca tgaagaaggc catcaacccc
     1141 tccatggcca gcggcgcgcc gaacacgtcg cccacgtagt ggctgtagta gctccagttc
     1201 atgccgaact cgaactccat cacgatgccg gtggccacgc ccatggcgaa gttgatgccg
     1261 aacagcgtgc cccagaactt ggtcatgtcg cgccagatgg ggcggcccgt catgacgtag
     1321 accgtctcca tgatcgccag cagcaacgac aggccgatcg tcaagggcac gaacaggaag
     1381 tggtacagcg ccgtgagcgc gaattgcaat cgcgacaggg cgacgatgtc aagttccatg
     1441 gggggtctcc atcttggctt ctgttctggg tggggattcg gggttcagtc ggggcgcagc
     1501 gaggcgagca tcgtatcgaa gccgggcgtg ccccggcgca gatcgcgcac gatgcggccg
     1561 tgctcgacct gcagcagccg gtcggccagc gcggcttcgc ggcgcaggtg agtggcgatg
     1621 agcagcgtgc gacgcccggt gtcggccgtg gcgtccaggc ggcgcagcac gtcgcgcgcg
     1681 gtcgctgcgt ccagcgcctc ggtgcattcg tccagcagcc acagcggccg gcgctgcagc
     1741 agcagccgcg ccagcgccag ccggcgcgcc tggccgcccg acaggccctg gccgccttcg
     1801 cccagcggtg tgtccaggcc gcgcggcagg ccgcgcacat cggcgcccag gccggcggcg
     1861 tcgagcgcgg ccatcagttc gtcatcggtg gcggcggggt cggccagccg caggttgtcg
     1921 cgcaggctgt cctggaacag atcgatgcgc tgcgtgagcc aggccgccgc cagcgcctcg
     1981 acacggccgg cccgtggcgc cagttcgccg gccaccagcg cgagcagggt ggacttgcca
     2041 gccccgctgg cgccgaccag cgcgagccgc tcgcccggcg cgatggccag cgccggcacg
     2101 acgaggacgt cgcccaggct gccggcatgc gcacagcgca cgccatcgag gcgcacggcc
     2161 ggcgccatcg agccgtccgc agcgcgggcc cggccttgcg caagcagcgg cgcctcatcg
     2221 ggcagctcca gcgcgggcgc aacccggcgc gcggccagca gcgcgcgccc ggcttccagc
     2281 gcgccgcggc gcagcgcgcc caagggctcg cccgcgccca gcgcgacgag cagcatcagg
     2341 gccgccacgg gtgcgccgat gcgcccctgc tccaccagcc aggccgcacc gagcagcacg
     2401 ccggccagcg ccagcgccgc cgcggccccg tgcccgaaca ggctgcgcac ctccaggcgg
     2461 ttgagtcgcc gatcggacgc ggcgatgcgc gcatcggccg cttcgatggc gcgttgctgg
     2521 gcctcgaggc gcccagccat ggccagctcg gtgcggcccg cgaccaagtc ggcggtgcgc
     2581 gcgcgcaggg cctccagcgc cgcgccgcgc cgcgccgcgg cctgcgccgc ctggcgtgcc
     2641 agcagccacg cgctgcccca gccgaccgcc aacagccagg cgaacaacgc caggccctgc
     2701 ctggcgtcct gcacgccgac gatcaggcct gccagcagcg ccgcgcccag ggtggcggcc
     2761 gcgggcacca gcaggcgcag gtacagggct tcgagcgcgt cgatgtcggc ggtcaggcgg
     2821 aacagcagcc gcgccggccg cagttgcagc tgccgcgccg cgtgcggccc ggaccaggcg
     2881 cgaaacagcc gctcgcgcag cgcggccagc acgccgagcg tggcatcgtg cgtgaccagg
     2941 cgctcgccat agcgcgagag cgtgcggccg agcgccagca ggcgaatgcc ggccgagggc
     3001 atgaacacgt cgaaggccag cgcgatcgcc acctgcgccc ccgccagcgc gctggccgtg
     3061 atgaaccagc ccgacagccc gagcaggccg atgcccatca gcaccgtcgt cacggccagc
     3121 agcagcccga gggccagtgg ccgaccctgc gcggcggcga aaagacgcag cacggcacgc
     3181 agcccggaag ccggccgcac aagggcgctc atgccgcctc ccgcaggtca gccgcatcca
     3241 gccgcaccac gcgatgcatc cgc
//
LOCUS       DASRDG010000446         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_428271, whole genome shotgun sequence.
ACCESSION   DASRDG010000446 DASRDG010000000
VERSION     DASRDG010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428271"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFA35_07440"
     CDS             <1..597
                     /locus_tag="VFA35_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZT56041.1"
                     /translation="SNADRIVCYDQIGIFSAPRARWLLRLFGHDAVAVLDGGLPQWRD
                     QGRPLQDGAPVAVAHTRYRTALRAGMLRGLGDLRDNLRTRRELVLDARSAARFEARVA
                     EPRPGQRGGHVPGARNLPYTELLTAQQTLLPPAELRARFARAGIDERSAVVTTCGSGV
                     TAAVLNLGLEVAGLGEGALYDGSWAEWGGRDDTPVETK"
     gene            549..1208
                     /locus_tag="VFA35_07445"
     CDS             549..1208
                     /locus_tag="VFA35_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZT56042.1"
                     /translation="MGGVGRAGRHAGGNEMIRLYDYLDSGNGYKIRLLLAQLERPYRH
                     VELDIMRGETRTSEFLAINPNGRIPALGLEDGRQLWESDAILWYLAEGSAYLPTDAFL
                     RAQVLQWMFFEQYSHEPYVATPRFILKHLPADTPRRAELPHRQALGRDALAVMERHLG
                     GHRFFVGDYYGIADIALYAYTHVAGEAGLELEPYPAVVDWLARVASQPRHVRISDRPA
                     A"
     gene            1285..1563
                     /locus_tag="VFA35_07450"
     CDS             1285..1563
                     /locus_tag="VFA35_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56043.1"
                     /translation="MTLVLLIAQLGAQAHAYSHLASAADGTQHHFRTLPCLECNSFAP
                     LLTLAGGLAPPPALAVADPTFERTPPAIANRSASICRAYRSRAPPVLS"
     gene            1664..>2014
                     /locus_tag="VFA35_07455"
     CDS             1664..>2014
                     /locus_tag="VFA35_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56044.1"
                     /translation="MAAAALLLACAPSRASEVDELRAQLEALKADYQAKMQALEQRLA
                     QLEAQAASAGAPGAVAPAAVAPGVAAPAYSGPPIPGSAEAAAAAGSGGVGGTGGAANS
                     ATAFNPAMSVILGGH"
BASE COUNT          297 a          701 c          682 g          334 t
ORIGIN      
        1 tcgaatgccg atcgcatcgt ctgctatgac cagatcggca tcttctccgc gccgcgcgcg
       61 cgctggctgc tgcggctgtt cggccatgac gcagtcgcgg tgctggacgg cggactgccg
      121 cagtggcgcg accaggggcg accgctgcag gacggagcac cggtggccgt tgcacatacc
      181 cgttatcgca cggcgctccg ggctggaatg ctgcgcgggc tgggtgacct gcgcgacaac
      241 ttgcgcacac ggcgcgagct cgtgctcgac gcgcgctcgg ccgcgcgctt cgaggcgcgc
      301 gttgccgagc cacgacccgg gcagcgtggc ggccacgtgc cgggtgcgcg caacctgccc
      361 tacaccgaac tgctgacggc gcagcagacg ctcctgccgc cggccgagct gcgcgcgcgc
      421 tttgcccgag cgggcataga tgaaaggagt gcggtagtca cgacctgcgg atcgggcgtt
      481 acggccgcag tgctgaacct cggcctcgaa gtggcaggtc tcggcgaggg tgccctgtac
      541 gacggctcgt gggcggagtg gggcgggcgg gacgacacgc cggtggaaac gaaatgatca
      601 ggctctatga ctacctcgat tccggcaacg gctacaagat ccgcctgctg ctggcacagc
      661 tcgagcggcc ttatcggcac gtcgaactcg acatcatgcg cggcgagacg cgaaccagcg
      721 agttcctcgc catcaatccg aacggccgca ttccggcact cgggctcgag gatgggcgtc
      781 agctgtggga gtcggacgcc atactctggt atctcgccga gggcagcgcg tacctgccca
      841 cggacgcctt cctgcgtgcg caggtgctgc aatggatgtt ctttgagcaa tacagccacg
      901 aaccttacgt ggccacgccg cgcttcatcc tgaagcacct gcccgccgac acgcctcgac
      961 gcgccgagct gccgcatcgc caggcgctcg gccgcgatgc cctcgcggtc atggagcgcc
     1021 atctgggcgg acaccgcttc ttcgtcggcg actactacgg cattgccgac atcgcgctct
     1081 atgcctatac gcatgtggcc ggtgaggccg ggctcgagct cgagccttac cccgcggtcg
     1141 tggactggct ggcgcgcgtc gcctcgcagc cgcgccatgt acgtatttcc gaccgcccgg
     1201 cggcgtgaaa tccgggggag tgctagactc ggcaagagtc gacgaaaagt gacaatgcga
     1261 ccaagacgct gcgcgacctg ggccatgacc ctggtcctgt tgatcgccca actgggcgcg
     1321 caggcgcatg cctacagcca tctcgcctcg gccgccgacg gcacgcaaca tcacttccgt
     1381 acccttcctt gcctcgagtg caacagcttc gcgccgttgc tgaccctggc gggcggcctt
     1441 gcaccgccac ccgcgcttgc cgtcgccgat ccgacgttcg agcgcacacc gccggcaatc
     1501 gcgaaccgat cggcctcgat ctgccgggcc taccgttcgc gcgcacctcc cgtcctgtcg
     1561 tagctctggc tcgatggacc gggcgcttcg gcgtccggcg gcccaggacg attgatcgag
     1621 gtgtgtcatg gatccgtgta ttcgtctcgc cggcttgcgt gccgtggcgg ccgcggcgtt
     1681 gttgctggcg tgcgcgccat cgcgcgcgtc cgaagttgat gagttgcgcg cccagctcga
     1741 ggcgttgaag gccgattacc aggcgaagat gcaagcactc gagcagcgac tcgcgcagct
     1801 cgaggcgcag gccgcatcgg ctggtgcgcc gggcgcagtc gcgcctgcgg cagtcgctcc
     1861 tggcgttgcc gctcccgcct attcgggccc gcccattccg ggctccgccg aagcggcggc
     1921 cgcggcaggc tcgggtggcg tgggtggcac cggaggggcg gccaactctg cgaccgcatt
     1981 caaccccgcc atgtcggtga tcctcggtgg ccac
//
LOCUS       DASRDG010000447         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1673449, whole genome shotgun sequence.
ACCESSION   DASRDG010000447 DASRDG010000000
VERSION     DASRDG010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673449"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /gene="gatB"
                     /locus_tag="VFA35_07460"
     CDS             <1..989
                     /gene="gatB"
                     /locus_tag="VFA35_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZT56045.1"
                     /translation="TPLLEIVTEPDMRSSREAVEYARALHTLVVWLGICDGNMQEGSF
                     RCDANVSVRKPGAPFGTRREIKNLNSFKFMQQAIDYEVQWQIDLLEDGGAVQQATVLF
                     DPDSGETRAMRSKEDAHDYRYFPDPDLPPLVIAADWIERVRTEMPELPRLMAARFERD
                     YALPAYDATLMTQSKDFAAFFEAAAQASGQPKLVANWLMGELSRRLNAQDLSIDASPV
                     KAPALAAMIARIADGTISNNGAKQVFDALWTGEGQDVDALIESRGLKQMSDAGELSAI
                     IDAVLAANPKSVEEFRAGKDKAFNALVGQAMKATRGKGNPSQVNELLKKKLG"
     gene            complement(958..1656)
                     /locus_tag="VFA35_07465"
     CDS             complement(958..1656)
                     /locus_tag="VFA35_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56046.1"
                     /translation="MRGLTAVPIEPLRIAALLLVACAATAAWGQATGPTPIFTCTDAS
                     GKRFTADRPIPECNARDQRMLNADGSVKRVRPPALTADERAVAEERERQALAERARQQ
                     EAVRRDRNLLARFPNEAAHRKARELALDDVRKSLRQSEARLAALANERKPLTSEAEFY
                     VGKPLPLKLKLALDANDASVDAQQSLVQNQQIEALRIEKRYDSELDRLKKLWAGAPPG
                     SLGVLTPTSSSAAR"
     gene            complement(1659..2339)
                     /gene="pyrE"
                     /locus_tag="VFA35_07470"
     CDS             complement(1659..2339)
                     /gene="pyrE"
                     /locus_tag="VFA35_07470"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZT56047.1"
                     /translation="MSTTARHDALAQDFVKFAVEAGVLRFGEFKTKAGRLSPYFFNSA
                     LFDDGAKLARLAEFYARRLLASGCGFDMLFGPAYKGITLAAASAVALAGLGRNVPFAF
                     NRKEAKAHGEGGTLVGAPVAGRVVIIDDVITDGASKRESVDMLRAAGGEPAAVLIALD
                     RMERGGSDDNLSAHSAVEDFERDYGMPVISIASLTDLLQYLQTTADPALKTHLAAVGA
                     YRERYGVR"
BASE COUNT          380 a          829 c          861 g          366 t
ORIGIN      
        1 gcacgccgct gctcgagatc gtcaccgagc ccgacatgcg ctcgagccgc gaagcggtcg
       61 agtacgcgcg cgcgctgcac acactcgtgg tctggctcgg catctgcgac ggcaacatgc
      121 aggaagggag ctttcgctgc gacgccaacg tctcggtgcg caagcccggc gcgccgttcg
      181 gcacgcggcg cgagatcaag aacctgaaca gcttcaagtt catgcagcag gcgatcgact
      241 acgaggtgca gtggcagatc gacctgctcg aagacggcgg cgcggtgcag caggccaccg
      301 tgctgttcga ccccgacagc ggcgaaaccc gggcgatgcg cagcaaggaa gacgcgcacg
      361 actaccgcta cttccccgac cccgacctgc cgccgctggt gatcgcggcc gactggatcg
      421 agcgcgtgcg caccgagatg cccgagttgc cgcgcctgat ggccgcgcgc ttcgagcgcg
      481 attacgcgct gcccgcgtac gacgcgacgc tgatgacgca gagcaaggac ttcgccgcgt
      541 tcttcgaggc cgccgcgcag gcgagcggcc agcccaagct ggtcgcgaac tggctgatgg
      601 gcgagttgtc gcgccgcctg aacgcgcaag acctgtcgat cgacgcgagc ccggtcaagg
      661 cgcccgcgct ggccgcgatg atcgcgcgca tcgccgacgg cacgatctcg aacaacggcg
      721 cgaagcaggt gttcgacgcg ctgtggacgg gcgagggcca ggatgtcgac gcgctgatcg
      781 aatcgcgcgg cctgaagcag atgagcgacg cgggcgagct ctccgcgatc atcgacgcgg
      841 tgctagccgc gaacccgaag tcggtcgagg agttccgcgc cggcaaggac aaggcgttca
      901 acgcgctcgt cggtcaggcg atgaaggcga cccggggcaa gggcaacccg agccaggtca
      961 acgagctgct gaagaagaag ttggggtgag cacgccgagc gagccgggcg gcgcgccggc
     1021 ccagagcttt ttgaggcgat cgagctcgct gtcgtagcgc ttttcgatgc gcagggcttc
     1081 gatctgctgg ttctgcacca gcgactgctg cgcgtcgacc gacgcgtcgt tcgcgtcgag
     1141 cgcgagcttc agcttcagcg gcagcggctt gccgacgtag aactcggctt cgctggtcag
     1201 cggcttgcgc tcgttcgcga gcgccgccag ccgggcctcg gactggcgca gcgacttgcg
     1261 cacgtcatcg agcgcgagtt cgcgcgcctt gcgatgcgcc gcctcgttcg ggaagcgcgc
     1321 gagcaggttg cggtcgcggc gcaccgcctc ctgctggcgc gcgcgctcgg cgagggcctg
     1381 gcgttcgcgc tcttcggcga cggcgcgctc gtcggccgtc aacgccggcg gacgcaccct
     1441 cttgaccgag ccatcggcgt tcagcatgcg ctggtcgcgc gcgttgcact cggggatcgg
     1501 ccggtccgct gtgaaccgct tgcccgacgc gtcggtgcac gtgaagatcg gcgtcgggcc
     1561 ggtcgcctgg ccccacgctg ccgtggcggc gcaggccacc aacagcagcg ccgcgatgcg
     1621 caaaggttcg atcggtaccg cggtcaggcc acgcattctc atcgcactcc gtatcgctcg
     1681 cggtatgcgc cgacagccgc gagatgggtc ttcagcgccg ggtcggccgt cgtctgcaga
     1741 tactgcagca ggtcggtgag gctcgcgatc gagatcaccg gcatgccgta gtcgcgctcg
     1801 aagtcttcaa cggccgagtg cgcggagagg ttgtcatcgc tgccgccgcg ctccatgcgg
     1861 tcgagcgcga tcagcaccgc cgccggctcg ccgccggccg cgcgcagcat gtcgaccgac
     1921 tcgcgcttcg aggcgccgtc ggtgatcacg tcgtcgatga tcacgacccg gcccgcgacc
     1981 ggtgcgccga ccagcgtacc gccctcgccg tgggccttgg cctccttgcg gttgaacgcg
     2041 aacggcacgt tgcggccgag gcccgcgagc gccacggccg aggccgccgc gagcgtgatg
     2101 cccttgtacg ccggcccgaa cagcatgtcg aagccgcagc ccgaggccag caggcgccgc
     2161 gcatagaatt ccgcgaggcg cgcgagcttg gcgccgtcgt cgaacagggc cgagttgaag
     2221 aagtacggcg agagccggcc ggccttggtc ttgaactcac cgaagcgcag cacgccggcc
     2281 tcgaccgcga acttgacgaa gtcttgcgcg agtgcgtcgt ggcgggcggt cgtgctcatc
     2341 cgaaggtcgt ctttcgtgtt ccgtcttgtt tcgctgaatc tgaacggcat ccgctcggcc
     2401 gccaacaagg gcttcgaggc ctgggccgag ggcgtg
//
LOCUS       DASRDG010000448         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1673511, whole genome shotgun sequence.
ACCESSION   DASRDG010000448 DASRDG010000000
VERSION     DASRDG010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673511"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..557
                     /locus_tag="VFA35_07475"
     CDS             30..557
                     /locus_tag="VFA35_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56048.1"
                     /translation="MTAGQALPSLDLLRVFVLTGPDTCSASESIINSLRGIGVPVVQV
                     GSTTCGKPYGFYPQDNCGTTYFSIEFRGINNQDFGDYPDGFTPANSRTASDPLTTVPG
                     CSVLDDFTHALGDPAEARLAAALNYRATNGASCPPPTGVAMMRANRPLSAVDAMLPPS
                     PLRQMRMLRILPSRR"
     gene            complement(547..1878)
                     /locus_tag="VFA35_07480"
     CDS             complement(547..1878)
                     /locus_tag="VFA35_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZT56049.1"
                     /translation="MISIGGIVGAGLFVGSSVTINAAGPAVVLSYLIAGVLVLLVMRM
                     LGEMAVALPQIRTFSDFARAGLGDWAGFVMGWMYWYFWIMVVPAEAIAGAQLLQQWIA
                     LPAWLIGVGLMAIMTGINLMSARSYGEFEFWFSLIKVSAIVAFILVAAAWAFGLTSDS
                     GPTWHNLVSGGGFAPRGYVPVLAAVTAVYFAIVGAEITVVAAAESREPVRAVARMSTA
                     VLTRIMLFYVGSILMIVAVVPWQQIVPGKSPFVAAMNVMHFNWANQAMTLIILTAVLS
                     CLNSAYYVTSRVLFALAAKGDAPAGLVQLNERGVPVRSVLMGSIAGFAGIAAATISPQ
                     GVFKFLVSASGAIMVFVYLMTAMAQIRLRNARPAAEQAALAVKMWFYPLGSYVAIVGM
                     IIVLLAMALTPEHAIEFRFSALALGLTLAAAWLRVRRRAAALRAAAPGTTA"
     gene            1868..>2501
                     /locus_tag="VFA35_07485"
     CDS             1868..>2501
                     /locus_tag="VFA35_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HZT56050.1"
                     /translation="MLIIEMWREARTRRNSGPPDPGDGSLCISRGSPAGSGALMMAHI
                     MDVGAVNHLPAVDGASPVPCANCGTPLAGRYCSTCGQRADVHAHSVGHFLHEFAEALT
                     HADSRVWATLVSLLQRPGFLTREYFAGRRARYLEPLRVYLFMSVLFLLLSAWLGGEGS
                     HLKVQVDQPKSIAECDQLQSNLVLGGKPMLPRLKAACRKIAADNGQHFGEN"
BASE COUNT          438 a          866 c          813 g          384 t
ORIGIN      
        1 cgttccactc gaccgcgcag ggtttttcgg tgactgcggg gcaggcgctg ccttcgctgg
       61 acctgctgcg ggtgttcgtg ctgaccggcc ccgatacctg ctcggccagc gagtccatca
      121 tcaacagcct gcgcggcatc ggcgtacccg tggtccaggt cggttcgacg acctgcggca
      181 agccctacgg cttctatccg caggacaact gcggcacgac ctacttctcg atcgaattcc
      241 gcggcatcaa caaccaggat ttcggcgact atccggacgg cttcacgccc gccaactcga
      301 ggacagccag cgatccgctg accacggttc ccggctgttc ggtgctcgac gacttcacgc
      361 acgcgctcgg cgatccggcc gaggcgcggc tggcggcggc gctcaattac cgtgccacga
      421 acggcgccag ctgcccgccg cccacgggcg tggccatgat gcgcgccaat cggccattgt
      481 cggcggtcga tgcgatgttg ccgccttcgc cgctgcggca gatgcgcatg ctgcggatac
      541 ttccgtctag gcggtagtgc ccggtgccgc ggcgcgcagt gccgccgcgc gccggcgtac
      601 gcgcaaccag gcggcggcga gcgtcagccc cagtgcgagt gcgctgaacc tgaattcgat
      661 cgcgtgctcg ggcgtgagcg ccatcgccaa cagcacgatg atcatgccga cgatggctac
      721 atagctcccc agcggataga accacatctt caccgccagt gccgcctgct cggcagccgg
      781 gcgcgcattg cgcaggcgga tctgcgccat cgccgtcatc aggtacacga acaccatgat
      841 ggcgccggaa gcgctgacga gaaacttgaa tacgccctgc ggcgatatcg tggcggcggc
      901 aatgccggcg aagcccgcga tgctgcccat cagcaccgag cgcactggaa cgccgcgctc
      961 gttgagctgc accaggcccg cgggcgcatc gcccttggcg gccagcgcga acagcacgcg
     1021 cgaagtcacg tagtaggcgg agttcaggca ggagagcacc gccgtcagga tgatgagcgt
     1081 catggcctgg ttcgcccagt tgaagtgcat gacgttcatc gcggccacga acggtgactt
     1141 gccgggcacg atctgctgcc acggcacgac cgcgacgatc atgaggatcg atccgacgta
     1201 gaacagcatg atgcgcgtca ggaccgcggt cgacatgcgc gccaccgcgc gcaccggctc
     1261 gcgcgattcc gccgcggcca cgaccgtgat ctcggcgccg acgatggcga agtagacagc
     1321 ggtcacggcc gcaagcacgg gcacatagcc gcgcggcgcg aagccgccgc cgctgacgag
     1381 gttgtgccag gtcgggccac tgtccgaagt caatccgaac gcccaggccg cggcgaccag
     1441 gatgaaggcg acgatcgccg agaccttgat gagcgaaaac cagaattcga actccccata
     1501 ggaacgcgcg gacatcaggt tgatgccggt catgatcgcc atcaacccga cgccgatcag
     1561 ccaggcgggg agcgcgatcc attgctgcag cagttgtgcg ccggcgatgg cttcggccgg
     1621 cacgaccatg atccagaagt accagtacat ccaccccatc acgaatccgg cccagtcgcc
     1681 gaggccggcg cgcgcgaaat cgctgaaagt gcgtatctgc ggcagcgcca ctgccatctc
     1741 acccagcatg cgcatgacga gcagcaccag gacgccggcg atcaggtagc tcagcaccac
     1801 cgccggcccg gcggcattga tcgtgacgct gctgccgacg aacaggccgg cgccgacgat
     1861 gccaccgatg ctgatcatcg agatgtggcg tgaggcgagg acccgccgca actcggggcc
     1921 gcccgatccg ggcgatggtt cgctgtgcat ctctcgaggt tcccccgccg gtagcggcgc
     1981 gcttatgatg gcccatatta tggatgtcgg cgccgttaat caccttccgg ccgtcgatgg
     2041 cgcatcgccg gtgccctgcg ccaactgcgg caccccgctg gccggccgct actgcagtac
     2101 ctgcggccag cgggccgacg tccacgcgca ctcggtcgga cacttcctcc acgagttcgc
     2161 cgaggcgctc acgcacgccg actcgcgcgt ctgggcgacg ctcgtgtcct tgctgcagcg
     2221 gccgggattc ctgacgcgcg agtacttcgc cggccgacgc gcccgctatc tggaaccgct
     2281 ccgggtgtac ctgttcatga gcgtgctgtt cctgctgctg agcgcgtggc ttggcggcga
     2341 ggggtcgcac ctcaaggtgc aagttgatca gccgaagtcc atcgccgagt gcgaccagct
     2401 gcagtccaac ctggtactgg gcggcaagcc gatgctgccg cggctgaagg ccgcgtgccg
     2461 caagatcgcc gccgacaacg gccagcactt cggggagaac a
//
LOCUS       DASRDG010000449         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1450846, whole genome shotgun sequence.
ACCESSION   DASRDG010000449 DASRDG010000000
VERSION     DASRDG010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450846"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFA35_07490"
     CDS             complement(<1..564)
                     /locus_tag="VFA35_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56051.1"
                     /translation="MISTSTRTLVTTGLTLAGALAAAGAPSSAWAAACSATAGQTSFI
                     ANASTDDVTLGGIDSTQCVISGVNPAQGPDGNTSGFDAAFGDSWSLLSKGPGTATIGG
                     VTFTLGFSQAAGTWSLTTDQAATFDLVFAMHASDHSGAFLFDNEKTFAGVTQNGSWSI
                     QWLNNGGQTPGFSNVNFFVRDVGTTSPI"
     gene            809..1132
                     /locus_tag="VFA35_07495"
     CDS             809..1132
                     /locus_tag="VFA35_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56052.1"
                     /translation="MNQRTSLAALALLAALGLGAAGAARADDPGADSARRERMDRAYE
                     DYRNPQPGPVARAENSIKRGAHRAGSAIKRGAKKVGHAVGTGVHKTGEAIGRGGEKLQ
                     EKSAP"
     gene            complement(1152..1601)
                     /locus_tag="VFA35_07500"
     CDS             complement(1152..1601)
                     /locus_tag="VFA35_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZT56053.1"
                     /translation="MTIHEAFPYLRVEGAAKAIDFYTRAFGAVEQFRLTEPSGRVGHA
                     QLQLGPIVLMLSEAYPEFGLNAPRDGSVLGASIHLHVDDCDALAAHAVACGATLEMAP
                     ADQFYGERSCRVRDPFGHQWLIGHEIEKVAPEEMQRRYDELMAKGGW"
     gene            complement(1598..>2208)
                     /locus_tag="VFA35_07505"
     CDS             complement(1598..>2208)
                     /locus_tag="VFA35_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZT56054.1"
                     /translation="WVDEALHPIPKNIYGATKLAAEDLCSLFHRQHGMPCIVLRTSRF
                     FPEPDDQQDVRERYAPANVQVNELLNRRVDIEDVLGAQLCAIERAAQIGFGRYIVSAT
                     TPFTRDELRSLRDELPAVVARHVPQYVAEYARRGWAMAPGIDRVYVNTKARAELGWRP
                     RHDFASAINALRGGGDHRSALARQVGAKGYHSTLHGHSEELR"
BASE COUNT          328 a          755 c          789 g          336 t
ORIGIN      
        1 aatcggcgag gtcgtcccga cgtcgcggac gaagaagttg acgttcgaga agcccggggt
       61 ctggccgccg ttgttcagcc actggatcga ccagctgccg ttctgcgtca cgccggcgaa
      121 tgtcttctcg ttgtcgaaca ggaacgcgcc gctgtggtcg gacgcgtgca tcgcgaacac
      181 caggtcgaag gtcgcggcct gatcggtcgt cagcgaccag gtgccggcgg cctgcgagaa
      241 gccgagcgtg aacgtcacgc cgccgatcgt cgccgtgccc ggccccttgc tgagcaacga
      301 ccagctgtcg ccgaacgcgg catcgaagcc gctcgtgttg ccgtccggcc cctgggccgg
      361 attgacgccc gagatcacgc actgggtcga gtcgatgccg cccagcgtca cgtcgtcggt
      421 cgaggcgttg gcgatgaagc tggtctgccc ggcggtggcg ctgcaggccg ccgcccacgc
      481 gctgctcggc gcgccggccg ccgccagcgc gccggcgagc gtcaagccgg tggtcacgag
      541 tgtgcgggtc gaagtcgaaa tcatggtctg ctcctgttgc tgaacgaacg ccgttgcgtc
      601 gtcgttgcag gacaccttag ggcctgctcg cagcggcacg accccgcgaa ccgggggttc
      661 gtgttagcgc ccgtcacaga cgcaagcagg ggtctggatt tggtcgcttt cgtaggaagt
      721 gaccgacaag ccgccgcgtt gcgcgccggt gttcgaagat cgatgcggcg cctaacgtgg
      781 cgtcgtgtca acaccgacag gaaccgccat gaaccaacga acttctcttg ccgcgctcgc
      841 attgctggcg gccctcggcc tcggcgccgc cggcgcagcc cgcgccgatg acccgggcgc
      901 cgacagcgcc cgcagggagc gcatggaccg cgcctacgag gactaccgca acccgcagcc
      961 cgggccggtg gcgcgcgccg aaaactcgat caagcgcggc gcgcaccgcg ccggcagcgc
     1021 gatcaagcgt ggcgccaaga aggtcggcca cgcggtcggc accggcgtgc acaagaccgg
     1081 cgaggcgatc ggccgcggcg gcgagaagct gcaggaaaag tcggcgccgt gaggccgcgg
     1141 tcgcgcggtc ctcaccaccc gcccttggcc atcagttcgt cgtagcggcg ctgcatctct
     1201 tcgggcgcga ccttctcgat ctcgtggccg atcagccact ggtgcccgaa cgggtcgcgc
     1261 acgcggcacg agcgttcgcc gtagaactgg tcggccggcg ccatctccag cgtcgcgccg
     1321 caagcgacgg cgtgcgccgc gagcgcatcg caatcgtcga catgcaggtg gatcgacgcg
     1381 ccgagcacgc tgccgtcgcg tggcgcgttc aggccgaact cggggtaggc ctcgctgagc
     1441 atcagcacga tcgggccgag ttgcagctgg gcgtggccga cgcgccccga cggttcggtc
     1501 agccggaact gctcgaccgc gccgaacgcg cgcgtgtaga aatcgatcgc cttcgctgcg
     1561 ccttcgacgc gcaggtaggg aaacgcttcg tggatggtca tcgcagctcc tcggagtgtc
     1621 cgtgcagggt cgagtgatag cccttcgcgc cgacctggcg cgccagcgcg ctgcggtggt
     1681 cgccgccgcc gcgcagcgcg ttgatcgccg acgcgaagtc gtgccgcggc cgccagccga
     1741 gctcggcgcg cgccttggtg ttgacgtaga cgcggtcgat gccgggcgcc atcgcccagc
     1801 cgcgcctggc gtactcggcc acgtactgcg gcacatggcg tgccaccacg gccggcagtt
     1861 cgtcgcgcag cgagcgcaac tcgtcgcgcg tgaacggcgt ggtcgcgctg acgatgtagc
     1921 ggccgaagcc gatctgcgcg gcgcgctcga tcgcgcacag ctgggcgccg agcacgtctt
     1981 cgatgtcgac gcgccggttc agcagttcgt tgacctgcac gttggccggc gcgtagcgct
     2041 cgcgcacgtc ctgctggtcg tcgggctcgg gaaagaagcg cgaggtgcgc agcacgatgc
     2101 agggcatgcc gtgctggcgg tggaacaggc tgcacaggtc ttcagcggcg agcttggtgg
     2161 cgccgtagat gttcttcggg atcgggtgca gcgcctcgtc gacccaca
//
LOCUS       DASRDG010000450         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_181724, whole genome shotgun sequence.
ACCESSION   DASRDG010000450 DASRDG010000000
VERSION     DASRDG010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181724"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2023)
                     /locus_tag="VFA35_07510"
     CDS             complement(<1..>2023)
                     /locus_tag="VFA35_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZT56055.1"
                     /translation="DALEAFFLRGNLPLGKTDDANDIPSAELLEDRVAQALALVREVR
                     ALWAGWLTSVDALFPQLQDHSLRASWKTQLRAPLAQIFSGAEFTPILEECNAIHQRTL
                     KGRVWVALHMHAGDGNVHTNIPVNSDNYEMLQTAHRAVARIMRLARSLDGVISGEHGI
                     GITKLEFVTDEELAAFTDYKQRVDPEGRFNKGKLLRGAAAAAAEGAGPLHADLTNAYT
                     PSFGLMGHESLIMQQSDIGEIANSVKDCLRCGKCKPVCTTHVPRANLLYSPRNKILAT
                     SLLIEAFLYEEQTRRGVSLRHWEEFEDVADHCTVCHKCLAPCPVDIDFGDVSMNMRNL
                     LRKMGQKSFRPANAAAMFFLNATQPETIKIVRTAMVGVGFKAQRAANNFLRGLANKQS
                     AAPPSTVGRAPLKEQVIHFVNKKMPGGLPNKTARALLDIEDKEYVPIIRNPKTTNADT
                     EAVFYFPGCGSERLFSQVGLATQAMLWHAGVQTVLPPGYLCCGYPQRGSGDYDKAEKI
                     ITDNRVLFHRVANTLNYLDIKTVVVSCGTCYDQLQGYEFDKIFPGCRIIDIHEYLLEK
                     GITLGGAGGPDAAAPGYLFHDPCHSPMKLQEPMKTVKALMGEQVVKSERCCGESGTLG
                     VTRPDISTQIRFRKEEELKKGEAALRDSGQVGAKDNVKILTSCPSCLQGL"
BASE COUNT          285 a          667 c          696 g          375 t
ORIGIN      
        1 aggccctgca ggcagctcgg gcagctggtc aggatcttca cgttgtcctt cgcgccgacc
       61 tggccgctgt cgcgcaaggc cgcctcgccc ttcttcaatt cctcttcctt gcggaagcgg
      121 atctgcgtgg agatgtccgg ccgcgtcacg cccagcgtgc ccgactcgcc gcagcagcgc
      181 tcgctcttga ccacctgctc gcccatcagc gccttgaccg tcttcatcgg ctcctgcagc
      241 ttcatcgggc tgtggcaggg gtcgtggaag agatagccgg gcgcggccgc gtcgggcccg
      301 ccagccccgc cgagggtgat gcccttctcg agcaggtact cgtggatgtc gatgatgcgg
      361 cagccgggga agatcttgtc gaactcgtag ccctgcagct ggtcgtagca ggtgccgcag
      421 ctcaccacca cggtcttgat gtcgaggtag ttcagggtgt tggcgacgcg gtggaacagc
      481 acccggttgt cggtgatgat cttctcggcc ttgtcgtagt cgcccgagcc gcgctgcgga
      541 tagccgcagc acaggtaccc gggcggcagc accgtctgca cgccggcgtg ccagagcatc
      601 gcctgggttg ccaggccgac ctgcgagaac agccgctccg agccgcagcc cgggaagtag
      661 aagaccgcct cggtgtcggc gttggtcgtc ttcgggttgc ggatgatcgg aacgtattcc
      721 ttgtcctcga tgtcgagcag cgcccgcgcg gtcttgttcg gcaacccgcc cggcatcttc
      781 ttgttgacga agtggatcac ctgctccttg agcggcgcgc ggcccactgt cgacggcggc
      841 gccgcgctct gcttgttggc caggccgcgc aggaaattgt tggccgcgcg ctgcgccttg
      901 aagccgacgc cgaccatcgc cgtgcgcacg atcttgatcg tctccggctg ggtcgcgttg
      961 aggaagaaca tcgccgccgc gttggccgga cggaacgact tctgccccat cttgcgcagg
     1021 aggttgcgca tgttcatgga gacgtcgccg aagtcgatgt ccaccgggca cggcgccagg
     1081 cacttgtggc agacggtgca gtggtcggcg acgtcctcga actcctccca gtggcgcagc
     1141 gacacaccgc ggcgggtctg ctcctcgtag aggaacgcct cgatcagcag cgacgtcgcg
     1201 aggatcttgt tgcgcgggct gtagagcagg ttggcgcgcg gcacgtgggt ggtgcacacc
     1261 ggcttgcact tgccgcagcg caggcagtcc ttgaccgagt tggcgatctc gccgatgtcg
     1321 ctctgctgca tgatcagcga ctcgtggccc atcaggccga agctgggcgt gtaagcgttg
     1381 gtcaggtcgg catggagcgg gcccgcgcct tcggccgccg ccgcggcggc gccgcgcaga
     1441 agcttgccct tgttgaagcg gccctcgggg tcgacccgct gcttgtagtc ggtgaaggcg
     1501 gcgagctcct cgtcggtcac gaactcgagc ttggtgatgc cgatgccgtg ctcgcccgag
     1561 atcacgccgt cgaggctgcg cgccagccgc atgatccgcg ccaccgcccg gtgcgcggtc
     1621 tgcagcatct cgtagttgtc gctgttgacc gggatgttgg tgtgcacgtt gccgtcgccc
     1681 gcgtgcatgt gcagcgcgac ccacacgcgg cccttcagcg tgcgctggtg aatggcgttg
     1741 cactcttcga ggatcggcgt gaactcggca ccggagaaaa tctgggccag cggcgcgcgc
     1801 agctgggtct tccagctcgc gcgcagcgag tggtcctgca gttgcgggaa cagcgcatcg
     1861 acgctcgtaa gccagccggc ccacagcgca cgcacctcgc gcaccagtgc cagcgcctgc
     1921 gctacgcggt cttccagcag ctcggccgag gggatgtcgt tggcgtcgtc ggtcttgccc
     1981 agcggcaggt tgccgcgcag gaagaaggct tccagcgcgt cgg
//
LOCUS       DASRDG010000451         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_429020, whole genome shotgun sequence.
ACCESSION   DASRDG010000451 DASRDG010000000
VERSION     DASRDG010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429020"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFA35_07515"
     CDS             <1..241
                     /locus_tag="VFA35_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT56056.1"
                     /translation="LQPTFVGLRSAVSMALVIVIVAEMFIGSDNGLGHRIIDAQQVLN
                     VKSMYAAILAAGALGYALNVLFLIAERKIVHWSGR"
     gene            267..1088
                     /locus_tag="VFA35_07520"
     CDS             267..1088
                     /locus_tag="VFA35_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT56057.1"
                     /translation="MSTVLNAPVVPDVPERPFTPGPTGTHITIRGLTKYFAGWPLYEN
                     FDLDIPKHKIVSVFGPNGCGKSTLINMIAGLIPIDAGQILFDGKSLKDTKIGYVFQNY
                     REAMFPWMRTIDNIAYPLVLEGRSKAEVDRRMAELVASFDVKFDLKRYPYELSGGQQQ
                     TASIMRALAPNPEVLFLDEPFSALDFEMTLFIREKLQEVFMQTGTTMMLVSHDLEEAV
                     YLADQVLLLTKRPTKVAEILQYDEARPRTVATLSEPGFVAAKKLSLEIFQREVRR"
     gene            1120..1323
                     /locus_tag="VFA35_07525"
     CDS             1120..1323
                     /locus_tag="VFA35_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtzG-like protein"
                     /protein_id="HZT56058.1"
                     /translation="MTPAQIEAYVDAAAAALQLPLAAEHRPGVLHYFAFASGMAELVG
                     AHPLAITDDPAEAFVPIAPAGGR"
     gene            1320..>2464
                     /locus_tag="VFA35_07530"
     CDS             1320..>2464
                     /locus_tag="VFA35_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtzE family amidohydrolase"
                     /protein_id="HZT56059.1"
                     /translation="MNADELLQGSAAAMAAAVRSGACSATALVQASLLRICATDARVN
                     AFTDVTAQRALQAAAALDARLAVGDTTVQALPLLGVPFAVKNLFDVAGITTLAGSKIE
                     RDRPPAAHDGPLVTRLQAAGAVLVGALNMDEYAYGFTTENSHAGACHNPHDLTRSAGG
                     SSGGSAAAIAAGQVPVSLGSDTNGSIRVPSSLCGTFGLKPTYGRLPRHGSYPFVASLD
                     HLGPFARSALDLALVYDALQGFDAHDPGCVNRANEPTTDALGRGVEGLRIATLGGYFA
                     ENAGPEARAAVAKVASALDATRSVELPEVARARAAAFLISNAEGAALHLPDLRTRAAD
                     FEPLSRDRFLAGALLPAAWVQQAQRVRRWFALRAAELFEEVDVLLAPA"
BASE COUNT          394 a          857 c          840 g          373 t
ORIGIN      
        1 cctccagccg acctttgtcg gcctgcgctc cgcggtgtcg atggcactcg tgatcgtcat
       61 cgtggccgag atgttcatcg gctccgacaa cggcctcggc caccggatca tcgacgcgca
      121 gcaggtgctg aacgtgaaga gcatgtatgc ggcgatcctg gcggcggggg cgctgggcta
      181 tgcgttgaac gtgctgttcc tgattgcgga acggaagatc gtgcattgga gcgggcgatg
      241 atgacggcac caagggagtc gaagacatga gcaccgtcct gaacgctccg gtggtccccg
      301 acgtgcccga gcgccccttc acacccggcc caaccggcac gcacatcacg atccgcgggt
      361 tgacgaagta tttcgccggc tggccgctgt acgagaactt cgacctcgac attccgaaac
      421 acaagatcgt gtcggtgttc gggccgaacg gctgcgggaa gtcgacgctg atcaacatga
      481 tcgccgggct gatcccgatc gacgcggggc agatcctgtt cgacggcaag agtttgaagg
      541 acacgaagat cggctacgtg ttccagaact accgcgaggc gatgtttccg tggatgcgca
      601 cgatcgacaa catcgcgtac ccgctggtac tcgaaggccg atcgaaggcc gaggtcgatc
      661 ggcgcatggc cgaactggtc gcgagcttcg acgtcaagtt cgacctgaag cgctacccgt
      721 atgaactctc gggtggccag cagcagacgg catcgatcat gcgcgcgctc gcgccgaacc
      781 ccgaggtgct gttcctcgac gagccgttct cggcgctcga cttcgagatg acgctgttca
      841 tccgcgagaa gctgcaggaa gtgttcatgc agaccggcac gacgatgatg ctggtctcgc
      901 acgacctcga agaagcggtg tacctggccg accaggtgct gctgctgacg aagcggccga
      961 ccaaggtggc cgaaatcttg cagtacgacg aggcgcgacc gcgcaccgtc gccaccctgt
     1021 cggaacccgg cttcgtggcg gcgaagaagc tcagcctgga gatcttccag cgcgaggtgc
     1081 ggcgctagcg acgtgaagac atgaaaggct cgccgctcga tgacccctgc gcagatcgaa
     1141 gcctatgtgg acgcggcagc cgccgccctg caactgccgc tggccgccga acaccggccc
     1201 ggcgtgctgc attacttcgc gttcgcgtcg ggcatggccg agctggtcgg cgcgcacccg
     1261 ctcgcgatca ccgacgaccc ggccgaggcc ttcgtaccga tcgctcccgc cggcggccga
     1321 tgaacgccga tgaacttctg cagggcagcg ccgccgcgat ggcggcggcg gtgcgcagcg
     1381 gcgcttgcag cgcgaccgca ctggtgcagg ccagcctgct gcgcatctgc gcgaccgacg
     1441 cgcgcgtcaa cgccttcacc gatgtgaccg cgcagcgcgc actgcaggcc gcagcggcgc
     1501 tcgacgcgcg gctcgcggtc ggagacacca cggtgcaagc gctgccgctg ctcggcgtgc
     1561 cgttcgcggt gaagaacctg ttcgacgtcg ccggcatcac cacgctggcc ggctcgaaga
     1621 tcgagcgcga ccgcccgccc gccgcgcacg acggcccgct ggtcacgcgc ctgcaggccg
     1681 ccggcgcggt gctcgtcggc gcgctgaaca tggacgaata cgcctatggc ttcacgaccg
     1741 aaaactcgca cgcaggcgcc tgccacaacc cgcacgacct gacgcgcagc gccggcggct
     1801 cgtcgggcgg ctcggccgcg gcgatcgcgg ccgggcaggt gccggtctcg ctgggctcgg
     1861 acacgaacgg ctcgatccgc gtgccctcgt cgctgtgcgg caccttcggg ctgaagccga
     1921 cctacgggcg gctgccgcgc cacggcagct acccgttcgt cgccagcctc gaccacctcg
     1981 ggccgttcgc gcgcagcgcg ctggatctgg cgctggtcta cgacgcgctg cagggcttcg
     2041 atgcacatga cccgggctgc gtgaaccgcg cgaacgagcc gaccaccgat gcgctcgggc
     2101 gcggcgtcga gggcctgcgc atcgcgacgc tgggcggcta cttcgctgag aacgccgggc
     2161 ccgaggcgcg cgccgccgtt gccaaggtcg cgagtgcgct cgacgcgacc cgcagcgtcg
     2221 aactgccgga ggtcgcacgg gcccgtgccg cggccttcct gatcagcaat gccgaaggcg
     2281 cagcgctgca cctgcccgac ctgcgcacgc gcgccgccga cttcgagccg ctgtcgcgcg
     2341 accgctttct cgccggcgcg ttgctgcccg ccgcgtgggt gcaacaggcg cagcgcgtgc
     2401 ggcgctggtt cgcgctgcgc gccgccgagt tgttcgagga ggtcgacgtg ctgctcgcac
     2461 cggc
//
LOCUS       DASRDG010000452         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1220416, whole genome shotgun sequence.
ACCESSION   DASRDG010000452 DASRDG010000000
VERSION     DASRDG010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220416"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..906
                     /locus_tag="VFA35_07535"
     CDS             100..906
                     /locus_tag="VFA35_07535"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HZT56060.1"
                     /translation="MIPATWFAPLLPFAMLAALAACHGSQASSAVPPQPASPPPSPAV
                     SPPVFEQFGTMREVMHDGSTGGRVALAHVHERGCYAVGALAGLAGEVTVDDGVVYVER
                     CPGSTPGLGTFPPESPLPQDQQATLLSVARVAAWRDVPLADAVDDAALTTAVRNAIGA
                     PQQVGEPQPPVPFRVEGTVAEVTVHCVRGQSPTDPADRSKLPWTRTTGQPVPVTLIGF
                     YAPGREGELTHHGTALHVHAVLLIEGRRTAAHADKFRLQPGARLQLPAAR"
     gene            979..>2283
                     /locus_tag="VFA35_07540"
     CDS             979..>2283
                     /locus_tag="VFA35_07540"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HZT56061.1"
                     /translation="MPKRPLLVLLAPPLLLAQQLAQQVAQQPAAIDPDGVPGARIIAG
                     GGTLPAAVHARFVELAGGEHAHIVLMPGASATADDADARSKSLADWQREFPQARFAVL
                     HTRDRATADSDAFAEPLRTASGVWFGGGDQQRLADAYLGTGVERELQALLARGGVVGG
                     TSAGAAIQSRTMIADGNPVPVMSQGFDFVPHGIVDQHFVKRNRLPRLLAALHDHPGHF
                     GLGVDEGTAIEVRGRELRVFGDSKALLVLAAGAGRPQRVLELVAGDHADLVSWQRAAR
                     DRAGSPWPPVPMAAPVVPHGTLVIIGGGALPDAAVDRFVAAAGGKDAKVVIVPTALGE
                     PLRGEDPFAAVLRRKGVTDIRVVDCPRPGAVTDAALKPLADATAIWFGGGRQWHLCDA
                     YEGTKALAAFAGVLARGGVIGGSSAGATIQGEFLVRGNPLGNL"
BASE COUNT          270 a          902 c          826 g          285 t
ORIGIN      
        1 ccgcctgcgc cgcatcctca accaggtgtc gcgtgcgctc gaggacgcgc gcgaatcggc
       61 gccgatcgcc aacttcgtgc aggtcctcct gggggcgctg tgatccccgc aacgtggttc
      121 gcgccgctgc tgccgttcgc catgctggcg gcgctcgcgg cctgccacgg ctcgcaggca
      181 tcgtctgcgg tgccgccgca acccgcctcg ccgcctccct cgccggccgt ctcgccgccg
      241 gtgttcgagc agttcggcac catgcgcgaa gtcatgcacg acggcagcac gggcggccgc
      301 gtcgcgctgg cccacgtcca cgagcgcggc tgctacgcgg tcggcgcgct cgcgggcctc
      361 gccggcgagg tcaccgtcga cgacggcgtg gtgtacgtcg aacggtgtcc gggctcgacg
      421 ccgggcctcg gcaccttccc gccggaaagt ccgctgccgc aggaccagca ggcgacgctg
      481 ctgtcggtgg cgcgcgtcgc cgcgtggcgc gacgtgccgc tcgcggatgc ggtcgacgac
      541 gcggcgctga ccacggcggt gcgcaacgcc atcggcgcgc cgcagcaagt cggcgagccg
      601 cagccgccgg tgccgttccg cgtcgagggc acggtcgcgg aggtcaccgt gcactgcgtg
      661 cgcggccaga gcccgaccga ccccgccgat cgctcgaagc tgccgtggac gcgcacgacc
      721 ggacagcctg tccccgtcac gctgatcggc ttctacgcgc caggacgcga gggcgagctc
      781 acgcaccatg gcaccgcgct gcacgtgcac gcggtgctgc tgatcgaggg ccggcgcact
      841 gcggcgcacg ccgacaagtt ccggctgcag ccgggcgcca ggctgcagct ccccgccgcg
      901 cgctgacgcc gcgccggcat cacgcatcgc caccgcgcgc cgacacgact catcggggcg
      961 ccgctatcat ccgcgcccat gccgaagcgg ccgctcctcg tgctcctcgc gcctccgctc
     1021 ctcctcgccc agcagctcgc ccagcaggtc gcccagcagc ccgccgcgat cgatcccgac
     1081 ggcgtgcccg gcgcgcgcat catcgcgggc ggcggcacgc tgccggccgc cgtccacgcg
     1141 cgcttcgtcg agctcgccgg cggcgagcac gcgcacatcg tgctgatgcc cggcgcctcg
     1201 gcgaccgccg acgacgccga cgcgcgcagc aagtcgctgg ccgactggca gcgcgaattc
     1261 ccgcaggccc ggttcgcggt gctgcacacg cgcgaccgcg cgaccgcgga cagcgacgcg
     1321 ttcgcggagc cgctgcggac cgcgtccggc gtctggttcg gcggcggcga tcagcagcgg
     1381 ctggccgacg cctacctcgg caccggggtc gagcgcgaac tgcaggcgct gctcgcgcgc
     1441 ggcggcgtgg tcggcggcac ttcggccggc gccgcgatcc agagccgcac gatgatcgcc
     1501 gacggcaacc cggtgccggt gatgtcgcag ggcttcgact tcgtgccgca cggcatcgtc
     1561 gaccagcact tcgtgaagcg gaaccgcctg ccgcgcctgc tcgcggcgct gcacgaccat
     1621 cccggccact tcgggctcgg cgtcgacgaa ggcaccgcca tcgaggtgcg cggccgcgaa
     1681 ctgcgcgtgt tcggcgacag caaggcgctg ctcgtgctcg ccgcaggcgc gggccggccg
     1741 cagcgcgtgc tcgagctcgt ggccggcgac catgccgacc tcgtgagctg gcagcgcgcc
     1801 gcgcgcgacc gcgcgggctc cccgtggccg ccggtgccga tggcggcgcc ggtcgtgccg
     1861 cacggcacgc tcgtgatcat cggcggcggc gcgctgcccg acgccgcggt cgaccgcttc
     1921 gtcgccgccg caggcggcaa ggacgccaag gtcgtgatcg tgccgaccgc gctcggcgaa
     1981 ccgctgcgcg gcgaggaccc gttcgcggcc gtgctgcgcc ggaagggcgt caccgacatc
     2041 cgcgtcgtcg actgtccgcg gccgggcgca gtcaccgacg cggcgctgaa gccgctcgcc
     2101 gacgcgaccg cgatctggtt cggcggcggc cgccagtggc acctgtgcga cgcctacgag
     2161 ggcaccaagg cgctcgccgc gttcgccggc gtgctcgcgc gcggcggcgt gatcggcggc
     2221 agctccgcgg gcgccacgat ccagggcgag ttcctcgtgc gcggcaatcc actcggcaac
     2281 ctc
//
LOCUS       DASRDG010000453         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1674157, whole genome shotgun sequence.
ACCESSION   DASRDG010000453 DASRDG010000000
VERSION     DASRDG010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674157"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /gene="lolA"
                     /locus_tag="VFA35_07545"
     CDS             <1..559
                     /gene="lolA"
                     /locus_tag="VFA35_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HZT56062.1"
                     /translation="HADAVDTLREFIRDVKTGRAQFTQTVTSPDGAKKKVSTGSFEFA
                     RPNRFRFVYAKPFEQTIVADGHKVWIFDADLNQASSRKFASALGATPAALLAGGSLDK
                     DFDLAALPAKDGLDWVSATPKAKEGAFKSLRVGFRGKELAAVEIVDAFEQRSLLQFSQ
                     FAAGVSFPADAFRFTPPAGADVIEQ"
     gene            556..1878
                     /locus_tag="VFA35_07550"
     CDS             556..1878
                     /locus_tag="VFA35_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZT56063.1"
                     /translation="MTGDPLLPFDDELPAPLAERLRPQTLDEVVGQQHLLGPGKPLRA
                     AFESGRLHSMILWGPPGVGKTTLARLVAQGFDAQFIAISAVLGGVKDIRDAVERAQIA
                     KGGNTRTNRRTVVFVDEVHRFNKAQQDAFLPHVESGLFTFIGATTENPSFEVNSALLS
                     RATVHVLKSLNDTDLRELLVRAVALLAAPPLSEPARDRLIAYADGDARRLLNAYENLI
                     GMAGAVDAIDELLLEKSLGEQLRRYDKGGEQFYDTISALHKAVRGSDPDAALYWLVRM
                     LDGGVDPRYATRRLIRMASEDIGLADPRALRLALDAAETYERLGSPEGELALAQAVVY
                     LAVAPKSNAVYAAYNAVRAFVAQDGTRPVPLRLRNAPTALMKGLDYGRGYRYAHDEAG
                     GFAAGESYWPDGMTPTRFYAPVERGLEIRIGEKLAELRRLNEEAGPAV"
     gene            2016..>2736
                     /locus_tag="VFA35_07555"
     CDS             2016..>2736
                     /locus_tag="VFA35_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZT56064.1"
                     /translation="MDTFIQQIINGLVLGSVYALVALGYTMVYGIINLINFAHGEVLM
                     VGALTSWTVVTWLGASGLPGWLLLLISLIAAIVVCCALNYSIEKIAYRPLRNAPRLAP
                     LITAMGMSLLLQTLAMIIWEPTYKSYPILLPGAPYNIGGAVINNVQIMILVVTALTLS
                     GLMYLVNKTKLGRAMRATAENPRVASLMGVRPDTVISATFIIGATLAALAGVMYAANY
                     GTVQHDMGFLPGLKAFTAAVFG"
BASE COUNT          431 a          951 c          938 g          416 t
ORIGIN      
        1 gcacgccgat gcggtcgaca cgctgcgcga gttcatccgc gacgtgaaga ccggacgcgc
       61 gcagttcacg cagaccgtga cctcgcccga cggcgcgaag aagaaggtgt cgaccggcag
      121 cttcgagttc gcacgcccga accgctttcg cttcgtctac gccaagccct tcgagcagac
      181 gatcgttgcc gatggccaca aggtctggat cttcgatgcc gacctgaacc aggcgagttc
      241 gcgcaagttc gcgtccgcgc tcggtgcgac gccggccgcg ttgctcgcgg gcggttcgct
      301 cgacaaggac ttcgacctcg cggcgctgcc cgccaaggac ggcctcgact gggtcagcgc
      361 gacgccgaag gcgaaggagg gtgcgttcaa gtccttgcgc gtcggcttcc gcggcaagga
      421 actggcggcg gtcgagatcg tcgacgcgtt cgagcagcgc tcgctgctgc agttcagcca
      481 gttcgcggcc ggcgtcagct tcccggccga tgcgttccgc ttcacgccgc cggccggcgc
      541 cgacgtgatc gagcagtgac cggcgatccg ctgctgccgt tcgacgacga gttgcccgcg
      601 ccgcttgccg agcggttgcg cccgcagacg ctcgacgagg tcgtcggcca gcagcacctg
      661 ctcgggccgg gcaagccgct gcgcgcggcg ttcgaatcgg gccggctgca ttcgatgatc
      721 ctgtggggcc cgcccggcgt cggcaagacc acgctggcgc ggctggtcgc gcagggtttc
      781 gacgcgcagt tcatcgcgat ctcggcggtg ctcgggggcg tgaaggacat ccgcgatgcg
      841 gtcgagcgcg cgcagatcgc gaagggtggg aacacgcgca cgaaccggcg cacggtcgtg
      901 ttcgtcgacg aagtgcatcg tttcaacaag gcccagcagg acgcgttcct gccgcacgtc
      961 gagtcgggct tgttcacctt catcggcgcg accaccgaga acccgtcgtt cgaggtcaac
     1021 tcggcgctgc tctcgcgcgc gaccgtgcat gtgctgaagt cgctgaacga caccgacctg
     1081 cgcgagttgc tggtgcgcgc cgtcgcgctg ctggcggcgc cgccgctgag cgagcccgcg
     1141 cgcgaccgcc tgattgccta cgccgacggc gacgcccggc gattgctgaa cgcctatgag
     1201 aacctgatcg gcatggccgg cgcggtcgac gcgattgacg agctgctgct cgagaagtcg
     1261 ctcggcgagc agctgcgccg ctacgacaag ggcggcgagc agttctacga cacgatctcg
     1321 gcgctgcaca aggcggtgcg cggcagcgac ccggatgccg cgctctactg gctcgtgcgc
     1381 atgctcgacg gcggcgtcga cccgcgctac gcgacgcgcc ggctcatccg catggccagc
     1441 gaagacatcg gcctggccga cccgcgcgcg ctgcggctcg cgctcgacgc ggccgagacc
     1501 tacgagcgcc tcggctcgcc cgagggcgag ctcgcgctcg cacaggccgt ggtctacctc
     1561 gcggtcgcgc cgaagagcaa cgccgtctac gccgcctaca acgcggtgcg cgctttcgtc
     1621 gcgcaggacg gcacacggcc cgtgccgctg cgcttgcgca acgcgccgac cgcgctgatg
     1681 aagggcctgg actacggccg tggctatcgc tacgcgcacg acgaagccgg cggcttcgcg
     1741 gccggcgagt cgtactggcc cgacgggatg acgccgacgc ggttctatgc gccggtcgag
     1801 cgcggtctgg agatccgcat cggcgagaaa ctcgccgaac tgcggcggct caacgaggag
     1861 gccggcccgg cagtctgaac cggcaccggg aaagcgatgg ccggcgagcg cctgcgcgaa
     1921 cctagaatcc gctgcgcggc catggccgga atattgctac tggatgagac gcggaaaaca
     1981 agcccgacga cgggccacgc aggagtgaca agcgcatgga caccttcatc cagcagatca
     2041 tcaacggcct cgtgctcggc agcgtctacg cgctcgtggc gctcggctac acgatggtct
     2101 acggcatcat caacctgatc aacttcgcgc acggcgaggt gctgatggtg ggtgcgctca
     2161 cgagctggac cgtggtgacc tggctcggcg cgagcggcct gccgggctgg ctgctgctgc
     2221 tgatctcgct cattgccgcg atcgtcgtgt gctgcgcgct gaactacagc atcgagaaga
     2281 tcgcctaccg gccgctgcgc aacgcgccgc gcctggcgcc gctgatcacc gcgatgggca
     2341 tgagcctgct gctgcagacg ctggcgatga tcatctggga gccgacctac aagtcgtacc
     2401 cgatcctgct gccgggcgcg ccgtacaaca tcggcggcgc ggtgatcaac aacgtgcaga
     2461 tcatgatcct cgtcgtcacc gcgctcacgc tcagcggcct gatgtacctc gtcaacaaga
     2521 ccaagctcgg ccgcgcgatg cgcgccaccg ccgagaaccc gcgcgtggcc agcctcatgg
     2581 gcgtgaggcc cgacaccgtg atctcggcca ccttcatcat cggcgcgacg ctcgcggcgc
     2641 tggccggcgt gatgtacgcg gccaactacg gcaccgtgca gcacgacatg ggcttcctgc
     2701 ccggcctcaa ggccttcacg gccgcggtgt tcggcg
//
LOCUS       DASRDG010000454         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_523496, whole genome shotgun sequence.
ACCESSION   DASRDG010000454 DASRDG010000000
VERSION     DASRDG010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523496"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFA35_07560"
     CDS             <1..608
                     /locus_tag="VFA35_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZT56065.1"
                     /translation="AAAERAFAERLLRTGDRRLWFARGNCFFREGDLARAVWAFECAR
                     LGMPRDPELLANLALARQRLELGAGSQPFVDALVQLRARFTGGELALLAAAAMTVAAL
                     ALCFWWRRPAARWIGFLALAPGLALAAELLWLAPARAPAAIALGKLELSSEPRTGLPP
                     VATVAAGQRLELRSEGDGDWVRVEAEGRIGYARHELVGRIE"
     gene            complement(636..842)
                     /locus_tag="VFA35_07565"
     CDS             complement(636..842)
                     /locus_tag="VFA35_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56066.1"
                     /translation="MEYKLKGGLPQVPPALPQILMVVGAALAFLGLLVIYNPGLLAYM
                     VGGVFLLIGALLALIGYRARRLVG"
     gene            complement(842..1972)
                     /locus_tag="VFA35_07570"
     CDS             complement(842..1972)
                     /locus_tag="VFA35_07570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT56067.1"
                     /translation="MPAPEKASSTAVSVRPLRHPARFLDLQRRFYRGDPHFVPPMPAG
                     EAWQLDPRRNPYFEHGDVELLAAWRGELPVGRISAARDRLHDEFHGDRVGFFGHFEAQ
                     DGEAASALLAAAADWCRARGATTLRGPVDLSTNYRCGLLVDGEPGRPILMMPHNPRRY
                     AELLEGAGLRRAKDLLALHVTSEQMDLSRIGRIAERLRGKTRATLRRIDLRAFDREMA
                     VLWDLYHRIWERNWGFVPMTRAEFAAQARDLGKIANPALLHLAEVDGAPVGFIVALPD
                     VNEAIAACNGRLLPFGWWRFLRAMRKVRTIRVITLGVVPEHRKQGIEMLLMHAVTENG
                     LAAGFRACEASWILEDNADMLGPLQTLGYRPWRRYRVYERAL"
     gene            2043..3032
                     /locus_tag="VFA35_07575"
     CDS             2043..3032
                     /locus_tag="VFA35_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZT56068.1"
                     /translation="MRRHSCCPPALLACALLVGSLLVGACAARPAPSKVAEPAPDQYA
                     RLRAAEQLYRKDDPAFAAERDDLARDPVTAFWLTRLFVRDLMLAREGRETGRDYARVQ
                     FNDVDSATRVRTQTGDSDPLLRAAAGIKNPVEARALAQIDALGAAAAPCIVHDLGCHP
                     QAFVRELGVELLARIGRPALPAMEPLRASAREAERCVYVEALGGMPPDAASTALLSAF
                     VADDSEFTVRATAARALGRAGADAAPRLRQTLATDPDPFVRRTAAEALAAQRDAETAA
                     ALVGYLERCQQEHDRDGEATAQRALQTLSRTRTPRTPAAWRAWSQSLGFAGGR"
BASE COUNT          402 a         1167 c         1177 g          399 t
ORIGIN      
        1 acgcggccgc ggagcgcgcg ttcgcggagc gcttgctgcg caccggcgac cgccgcctct
       61 ggttcgcgcg cggcaactgc ttcttccgcg aaggcgatct ggcgcgcgcc gtgtgggcct
      121 tcgaatgcgc gcgcctcggc atgccgcgcg atcccgagct gctcgccaac ctcgcgctcg
      181 ccaggcagcg gctcgagctc ggcgccggca gccagccgtt cgtcgacgcg ctggtgcagc
      241 tgcgcgcgcg cttcaccgga ggcgaactcg ccctgctcgc ggcggcggcg atgacggtcg
      301 ccgcgctcgc gctctgcttc tggtggcggc ggcccgccgc gcgctggatc ggcttcctgg
      361 cgctcgcgcc cggcctggcg ctggcggccg agctcttgtg gctcgcgccc gcgcgcgcgc
      421 ccgcggcgat cgcgctcggc aagctcgagc tctcgtcgga gccgcgcacc ggcctgccgc
      481 cggtcgcgac ggtcgcggcg gggcagcggc tcgagctgcg cagcgagggc gacggcgact
      541 gggtgcgcgt cgaagccgag gggcgcatcg gctacgcaag gcacgaactc gtcggccgca
      601 tcgagtgagc gcgcgccccg cgcgtcagcc gaccatcagc cgacgaggcg ccgcgcgcgg
      661 tagccgatca acgccagcag cgcgccgatc agcaggaaca cgccgccgac catgtaggcc
      721 agcaggccgg ggttgtagat caccaggagc ccgaggaacg ccagcgcggc gccgacgacc
      781 atgaggatct gcggcagcgc cggcggcacc tgcggcaagc cacccttcag cttgtattcc
      841 atcagagcgc tctctcgtag acgcggtagc gacgccacgg ccggtagccg agcgtctgca
      901 gcggcccgag catgtcggcg ttgtcctcga ggatccagct cgcttcacag gcgcggaagc
      961 cggcggccag tccgttctcg gtcaccgcgt gcatcagcag catctcgatc ccctgcttgc
     1021 ggtgctcggg cacgacgccg agcgtgatca cgcggatcgt gcgcaccttg cgcatcgcgc
     1081 gcaggaaccg ccaccagccg aacggcagca gccggccgtt gcacgcggcg atcgcctcgt
     1141 tgacgtcggg cagcgcgacg atgaagccga cgggcgcgcc gtcgacctcg gcgaggtgca
     1201 gcagcgccgg gttcgcgatc ttgccgaggt cgcgcgcctg cgcggcgaac tcggcgcgcg
     1261 tcatcggcac gaagccccag ttgcgctccc agatgcggtg gtagaggtcc cacaggaccg
     1321 ccatctcgcg gtcgaacgcg cgcaggtcga tccggcgcag cgtggccctg gtcttgccgc
     1381 gcaggcgctc ggcgatgcgg ccgatgcgcg acagatccat ctgctcgctg gtgacgtgca
     1441 gcgccagcag atccttcgcg cgccgcaggc ccgcgccctc gagcagttcg gcgtagcggc
     1501 gcgggttgtg cggcatcatc aggatcggcc gtcccggctc gccgtcgacg agcaggccgc
     1561 agcggtagtt ggtcgacagg tcgaccggcc cgcgcagcgt cgtggcgccg cgcgcgcggc
     1621 accagtccgc ggccgccgcg agcagcgccg aggccgcctc gccgtcctgc gcctcgaagt
     1681 ggccgaagaa gccgacgcgg tcgccgtgga actcgtcgtg caggcggtcg cgcgcggcgc
     1741 tgatgcggcc gacgggcagc tcgccgcgcc acgccgccag caactcgacg tcgccgtgct
     1801 cgaagtacgg gttcctgcgc gggtcgagct gccacgcttc acccgccggc atcggcggga
     1861 cgaagtgcgg atcgccgcgg tagaaccgcc gctgcaggtc gaggaaccgc gccgggtggc
     1921 gcagcgggcg cacggacacc gcggtcgacg aggctttctc gggagctggc atccgggtag
     1981 acctacatcc tcgcggcggc ggccgccgtc cacaacgacg cagtcgctgc cgcgcgcaac
     2041 tcatgcgacg ccactcctgt tgtccgcctg cgctgctggc ctgcgcgctg ctggtcggct
     2101 cgctgctggt tggggcctgc gccgcccggc cggcgccgag caaggtcgcc gaaccggcgc
     2161 ccgaccagta cgcgcggctg cgcgcggcgg agcagctcta ccgcaaggac gatcccgcgt
     2221 tcgccgccga gcgcgacgac ctggcgcgcg atccggtgac cgcgttctgg ctcacgcgcc
     2281 tgttcgtgcg cgacctgatg ctcgcgcgcg aagggcgcga gaccggccgc gactacgcgc
     2341 gcgtgcagtt caacgacgtc gacagcgcga cgcgcgtgcg cacgcagacg ggcgacagcg
     2401 atccgctgtt gcgcgcggcg gcgggcatca agaacccggt cgaggcgcgc gcgctggcgc
     2461 agatcgacgc gctcggcgcg gcggcggcgc cgtgcatcgt gcacgacctc ggctgccatc
     2521 cgcaggcgtt cgtgcgcgag ctcggcgtcg agctgctggc gcgcatcggc cggcccgcgc
     2581 tgccggcgat ggaaccgctg cgcgcgagcg cgcgcgaagc ggagcgctgc gtctacgtcg
     2641 aggcgctcgg cggcatgccg cccgatgcgg cctcgacggc cctgctctcg gcgttcgtcg
     2701 ccgacgattc cgagttcacg gtgcgcgcca ccgcggccag ggcgctcggc cgcgcgggcg
     2761 ccgacgcggc gccgcggctg cgtcagacgc tcgccacgga tccggatccg ttcgtgcgcc
     2821 gcacggcggc cgaggcgctg gcggcgcaac gcgacgccga gacggccgcg gcgctggtcg
     2881 gctacctcga gcgctgccag caggagcacg accgcgacgg cgaggcgacc gcgcagcggg
     2941 cgctgcagac gctgtcgcgg acgcggacgc cgcgcacgcc ggcggcctgg cgcgcgtgga
     3001 gccagagtct cggtttcgcg ggcggcaggt agaacggacg gcgatggacg actggaagat
     3061 cacgcgcgac cgcaaggcct gcgaacagcc cggctgtccg ctgccgtcgg cgcggcactg
     3121 gttcgcggtg ctcgagttcc ccgcc
//
LOCUS       DASRDG010000455         4527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_776899, whole genome shotgun sequence.
ACCESSION   DASRDG010000455 DASRDG010000000
VERSION     DASRDG010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776899"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFA35_07580"
     CDS             <1..473
                     /locus_tag="VFA35_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZT56069.1"
                     /translation="SHEAADEALLLRIEGILGLSAKGVLHYADKRRGQRRSMRVERSG
                     AETRLEGFMLAGDISAEAWIKPLLQDELPAQAYGRLLLIPGAKAPVAVASRGKQVCSC
                     FDVSERQIEAALSGTTGTPDARLLQLQAQLKCGTNCGSCIPELKRLVQLRQKAA"
     gene            588..1541
                     /gene="ybiB"
                     /locus_tag="VFA35_07585"
     CDS             588..1541
                     /gene="ybiB"
                     /locus_tag="VFA35_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HZT56070.1"
                     /translation="MAITSYIKEIGRGKEGARALSEAQAHDLMSQVLDGSVTDLEIGA
                     FALAMRIKGESVAELTGFVRAAQERCITIAPARPTIVLPSYNGARKLPNLTPLLALLL
                     AQEGAQVLVHGPMRDAGRVTTAEIFHDLGLPCARNAEEIENGWLRREPVFIAIDDLCP
                     SLARLLAVRRVVGLRNSGHTVAKVLAPCTAGACLRVVNYTHPEYATMLGEFLAHTGGD
                     AVLLRGTEGEPVADPRRAPKLEVFIAGQARADLSRPAHEGVLTELPVLPRGFDAATTA
                     VYIQSVVSGEKPAPGPLTQQVECLIAALGAIDRNAPKEQIA"
     gene            1608..2852
                     /locus_tag="VFA35_07590"
     CDS             1608..2852
                     /locus_tag="VFA35_07590"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZT56071.1"
                     /translation="MAGPTSHLRFDAIVVGAGAAGLHCAAIAGQRDLRVLLIDHAEKV
                     AEKIRISGGGRCNFTNRDTTPANFLSANPHFCRSALARYTPADFIALLQRHCIAWHEK
                     HKGQLFCDESSDAVIAMLLRECEQGGVTRWQPCGVHTVRQSPAGFELDTDRGTVGAKQ
                     LVIATGGLSIPKIGATDFGYRLARQFGHAIVETRPALVPLSFDPVGWAPFAALSGLSL
                     EASIETGEGKARAAFLEDLLFTHRGLSGPAVLQISSFWRAGMPIRLDLAPGRDLGAEL
                     LEAKQSSRRKLANELAELIPKRLAEAWLAPTPELADRTLPEQRDRDLAQLAARLQAWA
                     LKPQATEGYRKAEVTAGGVDTRELSSQTMESRRIAGLYFVGEVVDVTGWLGGYNFQWA
                     WASAAACARALAPVCTPERLGL"
     gene            2984..3196
                     /gene="rpsU"
                     /locus_tag="VFA35_07595"
     CDS             2984..3196
                     /gene="rpsU"
                     /locus_tag="VFA35_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZT56072.1"
                     /translation="MTTIRVKENEPFDVALRRFKRTIEKLGLLTDLRAREFYEKPTAE
                     RKRKKAAAVKRHFKRVRSMQLPKKLF"
     gene            3286..3732
                     /locus_tag="VFA35_07600"
     CDS             3286..3732
                     /locus_tag="VFA35_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZT56073.1"
                     /translation="MTLKDRITEDMKTAMRAKDAPRLLAIRGLLAACKQREVDERIVL
                     DDAAVIAIIDKLVKQRKDSIAQFTAGNRPDLVEKEAAELGVLEAYLPQRMDAAAVAAE
                     VGAIVAELGAKGPGDMGKVMGAVKTRLAGKADMAAVSAAVKKALAP"
     gene            3729..4073
                     /locus_tag="VFA35_07605"
     CDS             3729..4073
                     /locus_tag="VFA35_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HZT56074.1"
                     /translation="MNVPQVQQLSTDLCVAPQLDADAMAWAAKSGFKSVVNNRPDFEG
                     GPDQPTSAVIEAAAKAAGLQYAWLPVLPAVQTPQEIARFAALLAELPKPILAFCRTGT
                     RSGKLYRAATEG"
     gene            complement(4078..>4527)
                     /locus_tag="VFA35_07610"
     CDS             complement(4078..>4527)
                     /locus_tag="VFA35_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZT56075.1"
                     /translation="FDDEGVLTKPRDVVKAGEVQGYFLSTYSARKLGMRTTGHAGGSQ
                     NLTLTSRLTQAGDDLPAMLKKLDRGLFVIELMGQGVNYVTGDYSRGAAGFWVEDGEIQ
                     FPVHEITIAGNLGSMFKGIRAVGADAYTMGSKTIGSVLIDRLKIAGL"
BASE COUNT          751 a         1607 c         1520 g          649 t
ORIGIN      
        1 cgtcgcacga ggcggccgac gaggcgctgc tgctgcgcat cgaaggcatc ctcgggttga
       61 gcgcgaaggg cgtgctgcac tacgccgaca agcgccgcgg ccagcgccgc agcatgcgcg
      121 ttgagcgcag cggcgccgag acgcggctcg aaggcttcat gctcgccggc gacatcagcg
      181 ccgaggcctg gatcaagccc ttgctgcagg acgaattgcc cgcgcaggcc tacggccggc
      241 tgctgctgat cccgggcgcg aaggcgccgg tggcggtcgc gtcgcgcggc aagcaggtct
      301 gcagttgctt cgacgtgagc gagcgccaga tcgaggccgc actgtccggc acgaccggca
      361 cgcccgacgc ccgactgctg cagttgcaag cccaactcaa gtgcggcacg aactgcggct
      421 cgtgcatccc cgagctgaag cgcctcgtcc agttgcgcca gaaggccgcc tgagagggcc
      481 acgccgcacc tcgcttcgtg ccgaccccct aaccccacag tgcaagcgag tgttgccgac
      541 ctccgcgtcg ggcctgaaaa ttgcgatccg cccgtgggga gacaagaatg gcgatcacaa
      601 gctacatcaa ggaaatcggc cggggcaagg agggcgcgcg cgcgctgagc gaggcccagg
      661 cgcacgacct gatgagccag gtgctcgacg gcagcgtcac cgatctcgag atcggcgcct
      721 tcgcgctggc catgcgcatc aagggcgaat cggtcgccga actcacgggc tttgtgcgcg
      781 ccgcccagga gcgctgcatc acgatcgcgc cggcgcgccc gaccatcgtg ctgccctcgt
      841 acaacggcgc gcgcaagctg cccaacctca cgccactgct cgcgctgctg ctcgcgcaag
      901 aaggtgcgca ggtgctggtg cacgggccga tgcgcgatgc cggccgggtc accactgccg
      961 agatcttcca cgacctcggc ctgccgtgcg cgcgcaatgc cgaagaaatc gagaacggct
     1021 ggctgcgccg cgaaccggtc ttcatcgcga tcgacgacct gtgcccgtcg ctggcgcgcc
     1081 tgctcgcggt gcgccgcgtc gtcggcctgc gcaactcggg gcacacggtc gcgaaggtgc
     1141 tggcgccctg caccgccggc gcgtgcctgc gcgtcgtcaa ctacacgcat cccgagtacg
     1201 cgacgatgct cggcgagttt ctcgcccaca ccggcggcga cgcagtgctg ctgcgcggca
     1261 ccgaaggcga gccggtcgcc gatccgcgcc gcgcgccgaa gctcgaggtg ttcatcgcgg
     1321 gccaagcgcg cgccgacctg tcgcgccccg cccacgaggg cgtgctgacc gaattgccgg
     1381 tgctgccgcg cggcttcgac gccgccacga ccgccgtgta catccagtcc gtcgtcagcg
     1441 gcgaaaagcc cgcgccgggg ccgctcaccc agcaggtcga gtgcctgata gcggcgctcg
     1501 gtgcgatcga ccgcaacgcg ccgaaggaac agatcgcgtg acgagaacca tcgcactcgt
     1561 cggcgacggg ccgggccacg ccgagcgcgc cgcataatcc gcggcccatg gccggcccga
     1621 cttcccacct ccgcttcgat gcgatcgtcg tcggcgccgg cgcggccggg ctgcactgcg
     1681 cggccatcgc cggccagcgc gatttgcgcg tgctgctgat cgaccacgcc gagaaggtcg
     1741 ccgagaagat ccgcatctcc ggcggcgggc gctgcaactt cacgaaccgc gacacgacgc
     1801 cggcgaactt tctttcggcc aacccgcact tctgccgctc ggcgctggcg cgctacacgc
     1861 cggccgactt catcgcgctg ctgcagcgcc actgcatcgc gtggcacgag aagcacaagg
     1921 gccagctgtt ctgcgacgag agcagcgacg cagtcatcgc gatgctgctg cgcgagtgcg
     1981 agcaaggggg cgtgacgcgc tggcagccgt gcggcgtgca cacggtgcgg cagtcgcccg
     2041 cgggcttcga gctcgacacc gaccgcggca cggtcggcgc gaagcagctc gtgatcgcga
     2101 ccggcggcct gtcgatcccg aagatcggcg cgaccgattt cggctaccgg ctggcgcgcc
     2161 agttcgggca cgcgatcgtc gagacgcgcc cggcgctcgt gccgctgagc ttcgacccgg
     2221 tcggctgggc gccgtttgca gcactttccg gcctctcgct cgaagcctcg atcgagaccg
     2281 gcgagggcaa ggcgcgcgcc gcctttctcg aggatttgct gttcacgcac cggggcttga
     2341 gcggcccggc ggtgctgcag atttcgagtt tctggcgcgc cggcatgccg atccggctcg
     2401 acctcgcgcc cggccgcgat ctcggcgcgg agttgctcga agccaagcag tcatcccgcc
     2461 gcaagctcgc aaacgagctc gccgagctga tcccgaaacg cctcgccgaa gcctggctcg
     2521 cgcccacgcc cgagctcgcc gaccgcacgc tgcccgagca gcgcgaccgc gacctcgcgc
     2581 aactcgcggc gcggctgcag gcctgggcgt tgaaaccgca ggcgaccgag ggctaccgca
     2641 aggccgaggt caccgccggc ggcgtcgaca cgcgcgaact cagttcacag acgatggaaa
     2701 gccggcggat cgccggcctg tacttcgtcg gcgaggtggt cgacgtgacc ggctggctcg
     2761 gcggctacaa cttccagtgg gcctgggcca gcgcggcggc ctgtgcacgc gcgctggcgc
     2821 ccgtttgcac gccggaacgc ctcgggctat aatcgacggt ctttgctggc aaaacaaacg
     2881 cgttgcgatc ggcggaacga cgaagagccg gaactccgga ccctccggct cccagggatc
     2941 tcgccgacac cgagcgcatt caatttcagg atcaaccttt tacatgacta cgattcgcgt
     3001 caaagaaaac gagccattcg acgtggcact gcgccgcttc aagcgcacca tcgagaagct
     3061 cggcctgctg accgacctgc gtgcccgcga gttctacgag aagcccaccg ccgagcgcaa
     3121 gcgcaagaag gcagccgccg tgaagcgtca cttcaagcgc gtccgcagca tgcagctgcc
     3181 gaagaagcta ttctgatttc gcggcgctga gcctgatcga ggctcgtcat tgcccagaaa
     3241 gcccgcgcat tgcgggcttt ttcgtttgtg cccctggagc cctccatgac cctcaaagac
     3301 cggatcaccg aagacatgaa gaccgccatg cgcgcgaagg acgcgccgcg cctgctcgcg
     3361 atccgcgggc tgctcgcggc gtgcaagcag cgcgaggtcg acgagcgcat cgtgctcgac
     3421 gacgcggccg tgatcgcgat catcgacaag ctcgtgaagc agcgcaagga ttcgatcgcg
     3481 caattcaccg ccggcaaccg gccggatctg gtcgagaagg aagcggccga actgggcgtg
     3541 ctcgaagcct atctgccgca gcgcatggat gccgccgcgg tggccgcaga ggtgggtgcg
     3601 atcgtcgccg agctgggcgc caagggtccg ggcgacatgg gcaaggtgat gggcgccgtg
     3661 aagacgaggc tggccggcaa ggccgacatg gcggcggttt cggcagcagt caagaaggcg
     3721 ctcgcaccat gaacgtgccg caagtccagc aactcagcac tgacctgtgc gtggccccgc
     3781 agctcgatgc cgatgcgatg gcctgggcgg cgaagagcgg cttcaagagc gtcgtgaaca
     3841 accggcccga cttcgagggc ggcccggacc agccgaccag cgcggtcatc gaggccgccg
     3901 ccaaggccgc cggcctgcag tacgcctggc tgccggtgct gccggcggtg cagacgccgc
     3961 aagagatcgc ccgcttcgcg gcgctgctgg ccgaactgcc gaagccgatc ctggcgttct
     4021 gccgcaccgg cacgcgctcg ggcaagctct accgcgccgc caccgaaggc tgagtcttca
     4081 gagaccggcg atcttcagcc gatcgatcag caccgagccg atcgtcttgc tgcccatggt
     4141 gtaggcatcg gcgccgaccg cccggatgcc tttgaacatc gagcccaggt tgcccgcgat
     4201 cgtgatctcg tggaccggga actggatctc cccgtcctcg acccagaagc cggccgcgcc
     4261 gcgcgagtag tcaccggtca cgtagttcac gccctgcccc atcagctcga tcacgaacag
     4321 gccgcggtcg agcttcttca gcatcgccgg caggtcgtcg ccggcttggg tcagccggct
     4381 cgtcagcgtc aggttctgcg aaccaccggc gtggccggtg gtgcgcatgc cgagcttgcg
     4441 agccgaatac gtcgacagga agtagccctg cacctcgccg gccttcacca cgtcgcgcgg
     4501 cttcgtcagc acgccttcgt cgtcgaa
//
LOCUS       DASRDG010000456         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1580426, whole genome shotgun sequence.
ACCESSION   DASRDG010000456 DASRDG010000000
VERSION     DASRDG010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580426"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /gene="pdxA"
                     /locus_tag="VFA35_07615"
     CDS             <1..663
                     /gene="pdxA"
                     /locus_tag="VFA35_07615"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZT56076.1"
                     /translation="AIVTAPIHKEALAAAGVEYPGHTEMLQALAAPGGTPPPVRMMLA
                     NDELRTVLVTIHLSLRQAIDAVTFDAVLDTVRIAHRSAARWGMAAPRIAVAGLNPHAG
                     EGGLFGDEELRVIGPAIAAARAEGIDASGPFAPDTVFMRARDAAGHPGEFDIVVAMTH
                     DHGLIPVKYLGVEHGVNVTLGLPFVRTSPDHGTAFDIAGTGRADASSLRAALAMARRL
                     AA"
     gene            complement(683..>2102)
                     /locus_tag="VFA35_07620"
     CDS             complement(683..>2102)
                     /locus_tag="VFA35_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HZT56077.1"
                     /translation="IRAHREELLQVSGGKLDHMVIDVVGSLFDQILSDSRVPPQMARQ
                     IARLQLPVLRVALNDASFFSSRRHPVRRFVNRIASLACAFDEFDDGPGKQFLERVREL
                     VQEIVDGDFDQIDIYAAKLSALEGFIAEQTHREVQQQGAASTLGAKEAELLQQQRYML
                     QLQGALTPLALPEYLRSFISQVWSQALVFAVSRDGPASDRATRYRRAARDLVMSIQPK
                     GSPPLRKKFLMQLPPLMRDLNEGLALIGWPEAAQREFFETLLPAHAESLRGAAQTELD
                     HNLMVKQLESIFGAPLPGHDAASAADPMDEVDAEQIAQRFSPEEAQRVGLVTESAVDW
                     SGTVDIDLGDETPAGAGADSAPAALPGIDIDLGLIGADPAEPTRGPQLMDHIRLGFAY
                     QMHMKDEWQKVRLAHVSAGRGFFVFTRGRKHQEMISMTSRMLARMCETNRFRAVESSY
                     LMERATQRARKQLAALGAPTRH"
BASE COUNT          283 a          732 c          772 g          315 t
ORIGIN      
        1 gcgatcgtca ccgcgccgat ccacaaggag gcgctggccg cggccggcgt cgaataccct
       61 ggccacaccg agatgctgca ggcgctggcc gcaccgggcg gcacgccccc gccggtgcgc
      121 atgatgctgg ccaacgacga gctgcgcacg gtgctggtga cgatccacct gtccttgcgc
      181 caggcgatcg acgcggtcac gttcgacgcc gtgctcgaca ccgtccggat cgcgcaccgc
      241 agtgccgcgc gttggggcat ggcggcgccg cgcatcgcgg tggccgggct gaacccgcat
      301 gccggcgagg gcggcctgtt cggcgacgag gagctgcgcg tcatcggccc ggcgatcgcg
      361 gcggcgcgcg ccgagggcat cgatgcgagc ggcccgttcg cgcccgacac ggtcttcatg
      421 cgcgcccgcg acgcggccgg gcacccgggc gagttcgaca tcgtcgtcgc gatgacgcac
      481 gaccatggcc tgatcccggt gaagtacctc ggcgtcgaac acggcgtgaa cgtcacgctc
      541 ggcttgccgt tcgtgcgcac cagccccgac cacggcaccg cgttcgacat cgccggcacc
      601 ggccgtgccg acgcgagcag cctgcgcgcg gcgctggcga tggcgcggcg gctcgcggcg
      661 tagcgccgca ggcgacttgt cgtcagtgcc gggtcggcgc gcccagcgcg gccagctgct
      721 tgcgggcgcg ctgcgtggcg cgctccatca ggtagctgct ctcgaccgcg cggaagcggt
      781 tcgtctcgca catgcgcgcg agcatgcgcg aggtcatcga gatcatctcc tggtgcttgc
      841 ggccgcgcgt gaatacgaag aagccgcggc cggcgctcac gtgcgcgagc cgcaccttct
      901 gccattcgtc cttcatgtgc atctggtacg cgaagccgag cctgatgtgg tccatcagct
      961 gcgggccgcg ggtcggctcg gccgggtcgg cgccgatcag gccgaggtcg atgtcgatgc
     1021 ccggcagcgc ggccggggcc gaatcggcgc cggcgcccgc gggggtctcg tcgccgaggt
     1081 cgatgtcgac cgtgcccgac cagtcgaccg cgctctcggt gaccaggccc acgcgctgcg
     1141 cctcctcggg cgagaaacgc tgggcgatct gctcggcgtc gacctcgtcc atcgggtcgg
     1201 cggccgaggc cgcgtcgtgg cccggcagcg gcgcgccgaa gatggactcg agctgcttga
     1261 ccatcaggtt gtggtcgagc tcggtctgcg ccgcgccccg cagcgactcg gcgtgcgccg
     1321 gcagcagcgt ctcgaagaac tcgcgctgcg ccgcttcggg ccagccgatc agcgcgaggc
     1381 cttcgttcag gtcccgcatc agcggcggca gctgcatcag gaacttcttg cgcagcggcg
     1441 gcgaaccctt gggctggatg ctcatcacca ggtcgcgggc ggcgcggcga tagcgcgtcg
     1501 cgcggtcgga ggcggggccg tcgcggctga ccgcgaacac cagcgcctgg ctccagacct
     1561 gcgagatgaa gctgcgcagg tactcgggca gcgcgagcgg cgtgagcgcg ccttgcagct
     1621 gcagcatgta gcgttgctgc tggagcagtt cggcctcctt ggcgccgagc gtgctcgccg
     1681 cgccttgttg ctgcacctcc cggtgggtct gttcggcgat gaagccttcg agcgccgaga
     1741 gtttggcggc gtagatgtcg atctggtcga agtcgccgtc gacgatctcc tgcaccagct
     1801 cgcgcacgcg ttcgagaaac tgcttgcccg ggccatcgtc gaattcatcg aaggcgcatg
     1861 ccagcgacgc gatgcggttg acgaagcgcc gcaccgggtg gcggcgcgac gagaagaagc
     1921 tggcgtcgtt cagcgccacg cgcagcaccg gcagctgcag gcgggcgatc tggcgtgcca
     1981 tctgcggcgg cacgcggctg tcggagagga tctggtcgaa caggctgccg acgacgtcga
     2041 tcaccatgtg gtcgagcttg ccgcccgaga cctgcagcag ctcctcgcgg tgggcgcgga
     2101 tc
//
LOCUS       DASRDG010000457         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_70052, whole genome shotgun sequence.
ACCESSION   DASRDG010000457 DASRDG010000000
VERSION     DASRDG010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70052"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFA35_07625"
     CDS             complement(<1..853)
                     /locus_tag="VFA35_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZT56078.1"
                     /translation="MKPLTAKPAAVPTAPPIAPAGKLEWRALIEWLREDGLISAEDAD
                     RTARRFAGGQSVQHPLVRIASVNLTRLGTTKTLDAEALTEWLAGRVGLPYLRIDPLRV
                     DVGRVAEIMSITYAERRRALPLTVGLNEVTIATCEPLDIGWVPEIEAHARKAVKLVLA
                     SPADLARYTTEFYTLSRSVRNAIKTGEVSALASFEQLVELGKTNKQLDANDQGVVQVV
                     DWLWQYAFDQRASDIHLEPRRERSVIRFRIDGVMHTVYQLPPGVMNAMIARVKLLGRM
                     DVVEKRRP"
     gene            complement(870..1652)
                     /locus_tag="VFA35_07630"
     CDS             complement(870..1652)
                     /locus_tag="VFA35_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HZT56079.1"
                     /translation="MSARKNKQPELAPATISEADGVRYLHLGTPWVQGAMRIRQSLAL
                     ELEYIQRMMVWMLLRPAEELTHGRAVQLGLGAAAITRFCHSTLRMRCTAVELNPSVIN
                     ACRLWFHLPADSARFELLNRDAADFVADADRIESAQALCVDLYDHDAASPVLDSAEFY
                     RGCHRVLADAGVMTVNLFGRDASFERSAQRIAAVFGAAQVVHLRPTKEGNTIVVALKH
                     APLPDRATLAARADNIERRFKLPAKKWLRMIRPLTPASAPEP"
     gene            complement(1649..>2071)
                     /locus_tag="VFA35_07635"
     CDS             complement(1649..>2071)
                     /locus_tag="VFA35_07635"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HZT56080.1"
                     /translation="GVASLRAQQYYGHPRNHFWSILGALWKLDLSAASYAERLAAARA
                     HGLGLWDVYASCERAGSLDSAIENACFNDLASLKQRAPRLEAIAHNGGESARAMRHTR
                     ALGLPVHRLPSTSPANASWRFERKLAAWRTVFEQHGVA"
BASE COUNT          289 a          704 c          746 g          332 t
ORIGIN      
        1 gcgggcggcg cttctcgacc acgtccatgc ggccgagcag cttgacgcgc gcgatcatcg
       61 cgttcatcac gcccggcggc agctggtaca cggtgtgcat cacgccgtcg atgcgaaagc
      121 ggatcacgct gcgctcgcgg cgcggctcga ggtggatgtc cgaggcgcgc tggtcgaagg
      181 cgtactgcca cagccagtcg acgacctgca ccacgccctg gtcgttcgca tcgagctgct
      241 tgttggtctt gccgagctcg accagctgct cgaagctcgc cagcgccgaa acctcgccgg
      301 tcttgatcgc gttgcgcacc gagcgcgaca gcgtgtagaa ctcggtcgtg tagcgcgcca
      361 ggtcggccgg gctcgcgagc accagcttca cggccttgcg cgcatgcgct tcgatctcgg
      421 gcacccagcc gatgtcgagc ggctcgcagg ttgcgatcgt cacctcgttc aggccgaccg
      481 tcagcggcag cgcgcggcgc cgctcggcat acgtgatcga catgatctcg gcgacgcggc
      541 cgacgtcgac acgcagcggg tcgatgcgca ggtacggcag gccgacgcgg ccggcaagcc
      601 attcggtgag cgcctcggcg tcgagcgtct tggtcgtgcc cagccgcgtc aggttgacgc
      661 tggcgatgcg caccagcggg tgctgcacgc tctggccgcc ggcgaagcgg cgcgccgtcc
      721 ggtcggcgtc ttcggcgctg atcagcccgt cttcgcgcag ccactcgatc agcgcgcgcc
      781 actccagctt gccggcgggg gcgatcggcg gcgcggtggg cacggcggcg ggcttggcgg
      841 tgaggggttt catgcgcatg ggactctgtc tacggttcgg gcgcgctggc aggcgtcagc
      901 ggccggatca tgcgaagcca ttttttcgcc ggcagcttga accggcgctc gatgttatcc
      961 gcgcgcgcgg ccagcgtggc ccgatcgggc agcggcgcgt gcttgagcgc aacgacgatg
     1021 gtgttgccct ccttcgtcgg gcgcaggtgc acgacctgcg ccgcgccgaa caccgcggcg
     1081 atgcgctgtg cgctgcgctc gaagctggcg tctcggccga acaggttgac cgtcatcacg
     1141 ccggcgtcgg cgagcacgcg gtggcagccg cggtagaact cggcgctgtc gagcacgggc
     1201 gacgcggcgt cgtggtcgta gaggtcgaca cacaacgcct gcgccgattc gatgcgatcc
     1261 gcgtcggcga cgaagtcggc agcgtcgcgg ttcagcaact cgaaacgcgc cgagtcggcg
     1321 ggcaggtgaa accacagccg gcaggcattg atcacgctcg ggttgagctc gaccgcggtg
     1381 cagcgcatcc gcagcgtcga atggcaaaag cgcgtgatcg ccgcggcgcc gaggccgagt
     1441 tgcaccgcac ggccgtgcgt cagttcctcg gccgggcgca gcagcatcca caccatcatg
     1501 cgctggatgt actcgagttc gagcgcgagc gattggcgga tgcgcatcgc gccctgcacc
     1561 cacggcgtgc ccaggtgcag gtagcgcacg ccgtcggcct cggagatggt cgcgggggcg
     1621 agttcgggtt gcttgttctt cctggcgctc atgcgactcc atgttgttcg aagaccgtgc
     1681 gccaggccgc cagcttgcgc tcgaagcgcc agctcgcgtt cgccgggctg gtcgagggca
     1741 atctgtgcac cggcaggccg agcgcgcgcg tgtggcgcat cgcacgcgcc gattcgccgc
     1801 cgttgtgcgc gatcgcttcg agccgcggcg cgcgctgctt caggctggcc agatcgttga
     1861 agcaggcgtt ctcgatcgcg ctgtcgaggc tgcccgcgcg ctcgcagctc gcgtacacat
     1921 cccacaggcc caggccgtgg gcgcgcgcgg cggcgagccg ttcggcatag ctcgccgcgc
     1981 tgagatcgag cttccacagc gcgcccagga tcgaccagaa gtggttgcgc gggtggccgt
     2041 agtactgctg cgcccgcagc gacgccacgc c
//
LOCUS       DASRDG010000458         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1329978, whole genome shotgun sequence.
ACCESSION   DASRDG010000458 DASRDG010000000
VERSION     DASRDG010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329978"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /gene="aceE"
                     /locus_tag="VFA35_07640"
     CDS             complement(<1..1597)
                     /gene="aceE"
                     /locus_tag="VFA35_07640"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZT56081.1"
                     /translation="MKNTPDADPVETAEWLDALEAVQQHRGTARTNFIVGRLVDLARR
                     DGVYVPQALTTAYCNTIPPEREERSPGDREIEHRLRSIIRWNAMAIILRANKESSELG
                     GHIASFQSAATLYDIGFGHFWHAANDTHGGDLIYFQGHCAPGIYARAFMEGRLTEEQL
                     LGYRQETAGRGISSYPHPWLMPDFWQFPTVSMGLGPLMAIYQARFLKYLQGRGLADTS
                     ARKVWAFMGDGEMDEPESLGAISLAGRERLDNLIFVINCNLQRLDGPVRGNGKIIQEL
                     EAVFRGAGWNVIKVVWGSGWDQLLAKDKSGLLLQRMEECVDGEYQDFKSKSGAYVREH
                     FFGKYEALKALVADMSDDEIWSLTRGGHDPVKVFAAYAAASKHTGQPTLILPKTVKGY
                     GMGESGEGQMIAHQAKKMTLDALRGFRDRFQIPVGDDQLADVPFIKLPDDSPEMKYLH
                     AWRAALGGYLPQRRRKSASLTIPPLSTFQRLLDSSGEREISTTMAFVQMLGTLVRDKN
                     IGKHVVPIVPDESRTFGMEGMFRQ"
     gene            complement(1594..>2299)
                     /locus_tag="VFA35_07645"
     CDS             complement(1594..>2299)
                     /locus_tag="VFA35_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HZT56082.1"
                     /translation="DAGDADAVLCGVNGGYRGHLQVLSDVIGQRDGVETMAAMNMLML
                     PERTLFICDTYVNVDPSPEQLTEITVLAADELRRFGITARVALLSHSCFGSDDSPSAQ
                     KMRRALALIRSLNPSFEVEGEMHGDAALSTDIREQVFPGSALTRDANLLVMPTLDAAN
                     ITFNVLKATAGNGITVGPILLGLAKPAHILTPTASVRRIVNMTALASVDAASQWASRT
                     ATSVLSRVNAAGRAAP"
BASE COUNT          338 a          777 c          790 g          394 t
ORIGIN      
        1 gctggcggaa catgccttcc atgccgaagg tgcgtgactc gtcgggcacg atcggcacca
       61 cgtgcttgcc gatgttcttg tcgcgcacca gcgtgccgag catctgcacg aaggccatgg
      121 tggtgctgat ctcgcgctcg ccgctgctgt cgaggagccg ctggaaggtc gacagcggcg
      181 ggatcgtgag cgatgccgac ttgcggcggc gctgcggcag gtagccgccg agcgccgccc
      241 gccacgcatg caggtacttc atctcggggc tgtcgtcggg cagcttgatg aagggcacgt
      301 cggcgagctg atcgtcgccg accgggatct ggaagcgatc gcgaaacccg cgcagcgcgt
      361 ccagcgtcat cttcttggcc tggtgcgcga tcatctggcc ttcgccggat tcgcccatgc
      421 cgtagccctt caccgtcttg ggcaggatca aggtcggctg gccggtgtgc ttgctcgccg
      481 ccgcgtaggc cgcgaacacc ttgaccggat cgtgcccgcc gcgggtcagg ctccagatct
      541 cgtcgtcgct catgtcggcg acgagcgcct tcagcgcctc gtacttgccg aagaagtgtt
      601 cgcgcacgta ggcgccgctc ttgctcttga agtcctggta ctcgccgtcg acgcactctt
      661 ccatgcgttg cagcagcagg ccgctcttgt ccttcgcgag cagctggtcc cagccgctgc
      721 cccagacgac cttgatgacg ttccagcccg cgccgcggaa gaccgcctcg agctcctgga
      781 tgatcttgcc gttgccgcgc accgggccgt cgagccgctg cagattgcag ttgatcacga
      841 agatcaggtt gtcgagccgc tcgcgcccgg cgagcgagat cgcgcccagc gattcgggct
      901 cgtccatctc gccgtcgccc atgaaggccc agaccttgcg cgccgaggtg tcggccagcc
      961 cgcgcccttg caggtacttc agaaagcgcg cctggtagat cgccatcagc ggcccgaggc
     1021 ccatcgagac ggtcgggaac tgccagaaat cgggcatcag ccacggatgc ggataggacg
     1081 agatgccgcg cccggccgtc tcctgccgat agccgagcag ctgctcttcg gtgagccggc
     1141 cttccatgaa cgcgcgcgca tagatgcccg gcgcgcagtg gccctggaag tagatcaggt
     1201 caccgccgtg ggtgtcgttg gcggcatgcc agaagtgccc gaagccgatg tcgtagagcg
     1261 tcgccgccga ctggaagctc gcgatgtgcc cgccgagttc cgacgactcc ttgttggcgc
     1321 gcaggatgat cgccatcgcg ttccagcgga tgatcgagcg cagccggtgc tcgatctcgc
     1381 ggtcgccggg ggagcgctcc tcgcgctcgg gcgggatcgt gttgcagtac gcggtggtca
     1441 gcgcctgcgg cacgtacacg ccgtcgcggc gcgccagatc gaccaggcgg ccgacgatga
     1501 agttcgtgcg cgcggtgccg cggtgctgct gcaccgcttc gagcgcgtcc agccattcgg
     1561 cggtctcgac ggggtcggcg tccggggtgt tcttcatggc gccgcccgcc cggcggcatt
     1621 gacccgcgac agcaccgagg tggcggtccg gctggcccac tgcgacgcgg cgtcgaccga
     1681 ggcgagcgcg gtcatgttga cgatgcgcct gacgctggcc gtcggcgtca ggatgtgggc
     1741 cggcttggcc agacccagca ggatgggccc gaccgtgatg ccgttgcccg cggtcgcttt
     1801 gagcacgttg aaggtgatgt tggccgcgtc cagcgtcggc atcaccagca ggttggcatc
     1861 gcgcgtcaac gccgagcccg ggaacacctg ctcgcggatg tcggtcgaca aggcggcatc
     1921 gccgtgcatc tcgccctcga cctcgaaact cggattgagc gagcgaatca gcgccagcgc
     1981 gcgacgcatc ttctgcgcgc tcggcgagtc gtcgctgccg aaacaggaat gcgacaacag
     2041 cgccacgcgc gcggtgatgc cgaagcggcg caactcgtcg gcggccagca cggtgatctc
     2101 ggtgagctgc tcggggctcg ggtcgacgtt gacgtaggtg tcgcagatga acagcgtgcg
     2161 ctccggcagc atcagcatgt tcatcgccgc catcgtctcg acgccgtcgc gctggccgat
     2221 cacgtcgctc agcacctgca ggtggccgcg gtagccgccg ttcacgccgc acagcacggc
     2281 gtcggcgtcg ccggcatcc
//
LOCUS       DASRDG010000459         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_182801, whole genome shotgun sequence.
ACCESSION   DASRDG010000459 DASRDG010000000
VERSION     DASRDG010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182801"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFA35_07650"
     CDS             <1..911
                     /locus_tag="VFA35_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZT56083.1"
                     /translation="AQLGPGKEACMKTCLVGFTPDHGGQEALALARTLSAGADCAIVV
                     CVVTPEVWGYPSLARVDQEYGAFLDQYAQETLDQARAALGDHPNVRYERVVAGSATEG
                     LTRAAEREGADFIVVGSARDSAVGRLLTGGVTDEILHVAPVPVAIAPRGFACTTPLAR
                     VSVGFSGGAAARGTVLRALGLAGRFKVPLRVLSFAVRDRQMFPSLGSHDAEAVVVQGW
                     LEQARNALQSVHGDIAVTGYAVETDVGVGPTWEDALAGLAWQDGEVLMLGSSRLGPLQ
                     RVFLGSNAAKIMRASRVPVIVLPRQS"
     gene            928..1884
                     /gene="speB"
                     /locus_tag="VFA35_07655"
     CDS             928..1884
                     /gene="speB"
                     /locus_tag="VFA35_07655"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HZT56084.1"
                     /translation="MTQHDFNQPLGGNAMPRFGGPATMMRLPAAASPQGLDACFVGVP
                     FDIGTSNRAGARFGPRQIRAESCLLRPYNMATRAAPFDSLQVADIGDVAINTFNLLDS
                     LKRIEAAYDTIAASGAKPLTMGGDHTIVLPILRALAKVHGPIGIVHVDAHADINDDMF
                     GERYAHGTPFRRAIEEGLINPKRMVQIGLRGTGYTAEDFDWPRSHGVRVVAAEECWYK
                     SLAPLMAEVRESFGRGPVYITFDIDGLDPSCAPGTGTPEVGGLTTIQGLEIIRGCRGL
                     DVIGGDLVEVAPPYDASGNTSLLAANLLYEMLCVLPGVSYRA"
     gene            1892..2854
                     /locus_tag="VFA35_07660"
     CDS             1892..2854
                     /locus_tag="VFA35_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HZT56085.1"
                     /translation="MINVTLQDVFVAQQRIAGSVTATPLVYSDKMSELRGADVWMKME
                     SLQRTGSFKIRGASNRLLALTPAERARGVITTSSGNFALGLGSAAKAAHVNAIVVVPT
                     NTPETKLESIRRLGVQLVMHGANYDIAYAHAKEIALKTGRTYVSSYEDPWVVAGQGTV
                     ALEAFLAEPHFDLILVPTGGGGLLSGVAMVAKSLNPGIKVIGLQTEASDPWLHSFRQR
                     KVVEVEYGQTLADGLLGEVGAANLDMVLRYVDDILVVKESEVAEAMHWMVKYHHQIVE
                     GSAAVGVAALLHHLPDIGGLKVLNIVTGSNVDSNRIAPILHAYE"
     gene            2867..3751
                     /locus_tag="VFA35_07665"
     CDS             2867..3751
                     /locus_tag="VFA35_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZT56086.1"
                     /translation="MRELPPLNALRVFDVAARCRSFSQAARQLCLTQGAVSRQIQTLE
                     QYYGFALFVRHPRQSLILTPEAQMLAPVVRESFARIEESTRRLTQRSAVLSLKMPTCA
                     MPWALPRVMRFRSAEPDIDLQLTTTFAHSVDFASEPFDAAIVYGESGAAGHVERLALF
                     AEVLTPVCAPSLRDARPLDRIDDLAGHTLLHPTRDHADWRRWLDAAGAPHLSADSGQT
                     FETMDLAMNAAARGFGVAIGDCTLMADDLAAGRLLCPFDLQLPTGQHYYLAIPERSAR
                     SDKLARLRDWIVAHREAV"
BASE COUNT          557 a         1373 c         1349 g          562 t
ORIGIN      
        1 ctgcgcaact cggacctggc aaggaagcgt gcatgaagac ctgtctggtc ggcttcaccc
       61 cggatcacgg cggccaggag gccctggcgc tggcgcgcac cttgtcggcc ggtgccgatt
      121 gcgccatcgt ggtctgcgtc gtcacccccg aggtctgggg ctacccgtcg ctggcgcgcg
      181 tcgaccagga gtacggcgcg ttcctcgacc agtacgcgca agagacgctg gaccaggccc
      241 gcgccgcgct cggcgaccac cccaacgtgc gctacgagcg cgtcgtggcc ggctcggcca
      301 ccgaggggct gacgcgcgcc gccgagcgcg agggcgccga cttcatcgtc gtcggctcgg
      361 cgcgtgacag cgcggtcggg cgcctgctga ccggcggcgt gaccgacgag atcctgcacg
      421 tcgcgccggt gccggtggcg atcgcgccgc gcggcttcgc ctgcaccacg ccgctggcgc
      481 gcgtcagcgt cggcttctcg ggcggtgcgg cggcgcgcgg cacggtgctg cgcgcgctcg
      541 gcctggccgg gcgcttcaag gtgccgctgc gcgtgctcag cttcgcggtg cgcgaccggc
      601 agatgttccc gtcgctgggc agccacgacg ccgaggcggt ggtcgtgcag ggctggctcg
      661 agcaggcccg caacgcgctg cagtcggtgc acggcgacat cgccgtcacc ggctacgccg
      721 tcgagaccga tgtcggcgtc ggcccgacct gggaagacgc gctggcgggc ctcgcctggc
      781 aggacggcga ggtgctgatg ctcggctcga gccgcctcgg gccgctgcag cgcgtgttcc
      841 tcggctcgaa tgcggcgaag atcatgcgcg cgtcgcgggt gccggtcatc gtgctgccgc
      901 gacagagtta aggagacgga gacccccatg acccaacacg acttcaatca gccgctgggc
      961 ggcaacgcga tgccgcgctt cggcggcccg gcgacgatga tgcggctgcc cgccgccgcc
     1021 tcgccgcagg ggctggacgc ctgcttcgtc ggcgtgccct tcgacatcgg caccagcaac
     1081 cgcgccggcg cgcgcttcgg gccgcgccag atccgcgccg agtcgtgcct gctgcggccc
     1141 tacaacatgg caacaagggc cgcgccgttc gactcgctgc aggtggccga catcggcgac
     1201 gtggcgatca acaccttcaa cctgctcgac agcctgaagc gcatcgaggc cgcctacgac
     1261 accatcgccg cctccggcgc gaagccgctg acgatgggtg gcgaccacac catcgtgctg
     1321 ccgatcctgc gcgcgctggc caaggtgcac gggccgatcg gtatcgtcca cgtcgacgcg
     1381 cacgccgaca tcaacgacga catgttcggc gagcgctacg cccacggcac gccgttccgc
     1441 cgcgcgatcg aggaaggcct gatcaacccg aagcgcatgg tgcagatcgg cctgcgcggc
     1501 accggctaca ccgccgagga cttcgactgg ccgcgcagcc acggcgtgcg cgtggtcgcc
     1561 gccgaggagt gctggtacaa gagcctcgcg ccgctgatgg ccgaggtgcg cgagagcttc
     1621 ggccgcggcc cggtctacat cacgttcgac atcgacgggc tcgacccgtc gtgcgcgccg
     1681 ggcaccggca cgcccgaggt cggcgggctg acgacgatcc agggcctgga gatcatccgc
     1741 ggctgccgcg ggctggacgt gatcggcggc gacctggtcg aggtcgcgcc gccttacgac
     1801 gcctcgggca acacctcgct gctggctgcc aacctgctgt acgagatgct gtgcgtgctg
     1861 ccgggcgtga gctaccgggc gtgaggccgc gatgatcaac gtcaccctgc aggatgtgtt
     1921 cgtcgcgcag cagcgcatcg ccggcagcgt gacggcgacg ccgctggtct actccgacaa
     1981 gatgagcgag ctgcgcggcg ccgatgtgtg gatgaagatg gagtcgctgc agcgcaccgg
     2041 ctcgttcaag atccgcggcg cgagcaaccg gctgctcgcg ctgacgccgg ccgagcgcgc
     2101 gcgcggcgtg atcaccacct cgtcgggcaa cttcgcgctc ggcctcgggt cggccgcgaa
     2161 ggcggcgcac gtcaacgcca tcgtcgtcgt gccgaccaac acgcccgaga ccaagctcga
     2221 gagcatccgc cgcctcggcg tgcagctggt gatgcacggc gccaactacg acatcgccta
     2281 cgcccacgcg aaggagatcg cgctgaagac cgggcgcacc tacgtctcga gctacgagga
     2341 cccgtgggtc gtcgcagggc agggcacggt ggcgctggag gcctttctgg ccgagccgca
     2401 cttcgacctg atcctggtgc ccaccggcgg cggcgggctg ctctcgggcg tcgcgatggt
     2461 cgccaagtcg ctcaacccgg gcatcaaggt gatcgggctg cagaccgagg cttccgaccc
     2521 gtggctgcat tcgttccgcc agcgcaaggt ggtcgaggtc gagtacggcc agacgttggc
     2581 cgacggcctg ctcggggaag tcggcgccgc caacctcgac atggtgctgc gctacgtcga
     2641 cgacatcctg gtcgtcaagg agagcgaagt cgccgaggcg atgcactgga tggtcaagta
     2701 ccaccaccag atcgtcgagg gctcggcggc ggtcggcgtc gcggcgctgc tgcaccacct
     2761 gccggacatc ggcggcctga aggtgctgaa catcgtgacc ggcagcaacg tcgactcgaa
     2821 ccggatcgcg ccgatactgc atgcgtatga ataggggtcg acaggcatgc gggaactgcc
     2881 gccgctgaac gcgttgcgcg tgttcgacgt cgccgcgcgc tgccgcagct tctcgcaggc
     2941 ggcgcggcag ctgtgcctga cgcagggcgc ggtgagccgg cagatccaga cgctcgagca
     3001 gtactacggc ttcgcgctgt tcgtgcgcca tccgcgccag agcctgatcc tcacacccga
     3061 ggcgcagatg ctcgcgccgg tggtgcgcga gagcttcgcc cgcatcgagg agtcgacgcg
     3121 ccggctgacg cagcgcagcg cggtgctgtc gctgaagatg ccgacctgcg cgatgccgtg
     3181 ggccttgccg cgcgtgatgc gctttcgcag cgccgagccc gacatcgacc tgcagctgac
     3241 gacgaccttc gcgcacagcg tcgatttcgc cagcgagccc ttcgatgcgg cgatcgtcta
     3301 cggcgagtcg ggggcggccg gccacgtgga gcggctggcg cttttcgccg aggtgctgac
     3361 gccggtgtgc gcgccgtcgc tgcgcgacgc ccgcccgctc gatcgcatcg acgacctggc
     3421 cgggcacacg ctgctgcacc cgacgcgcga ccacgccgac tggcgccgtt ggctcgacgc
     3481 cgcgggcgcc ccgcacctga gcgccgacag cggccagacc ttcgagacca tggacctggc
     3541 gatgaacgcc gcggcgcgcg gcttcggcgt ggccatcggc gactgcacgc tgatggccga
     3601 cgacctcgcc gccggccgcc tgctgtgccc gttcgacctg caattgccca ccgggcagca
     3661 ctactacctc gcgatcccgg agcgcagcgc gcgctcggac aagctggcgc gcctgcgcga
     3721 ctggatcgtc gcgcaccgtg aggcggtctg agccgcctct cttccttcct gtctctcttc
     3781 cttcacgctt ctcgaaaacg accatgacca ttcagcgcat caatcccggc aagcgccttt
     3841 g
//
LOCUS       DASRDG010000460         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_641177, whole genome shotgun sequence.
ACCESSION   DASRDG010000460 DASRDG010000000
VERSION     DASRDG010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641177"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..743)
                     /locus_tag="VFA35_07670"
     CDS             complement(18..743)
                     /locus_tag="VFA35_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZT56087.1"
                     /translation="MLALLGLPAATLTAQVEVNGGDVKIRAGDTQISSANGDVDIKAA
                     SSGKARIDASHDGSAHIDAGAAASGGARSRQNVHGANVNGVIVNAAHGTDAVSEQTID
                     GHTTTTTGNSRIVTSNGVTTVTGDGTTQATASSGAASVPGVVTKGVHSYANGELANTN
                     FSGQALVRVSFANATLTEADFSRADLRGADFANAGLARARFAGANLTGANLANAELED
                     TDFSGAIWTDGRVCGAGSRGACR"
     gene            complement(801..1955)
                     /locus_tag="VFA35_07675"
     CDS             complement(801..1955)
                     /locus_tag="VFA35_07675"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT56088.1"
                     /translation="MTTRTTRGRAWAAALAAAIVFACADGVGEVILKNKPPPPTDDAV
                     AAGNATDGSPQSLLRSAWQAYKGRRGGKPDYKTAFADFDRAAQLGDARAMLGAGYLLT
                     KGLGAARNPQAAREYLNGAEQAGYARAAYLLSLLDESLATPQSLVAAREARERAAARG
                     DAPAANALANGYLRSGQRPTALLWYRQAQLAGSASAPRNANRVQRQAQAGEPALQPID
                     ELRAASAAGDANATYELATRYHRGLGVAVNYGEAIRLYRIAAHGGSVAAQRMIAMVSS
                     RSTEAVPVDPQWMVELGQLSTDAGETRPGLGDLLEDDDPLSDLPSLKPGAKETLPGTT
                     VRNQRYIPPGTLGLGDSIAAPAAPKNGATLYDADAAARSRSRLSTGKPQQ"
     gene            complement(1987..>2750)
                     /locus_tag="VFA35_07680"
     CDS             complement(1987..>2750)
                     /locus_tag="VFA35_07680"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HZT56089.1"
                     /translation="NGMAPDEQARLFQTGLRSMGYYEGAIDGQTSPALREAIVLYQAD
                     HQATASGDIDFETYERLMKNYVASDSQNGFVRIGWARDNGDKGRGFAQKPRNGQPAFD
                     SANANPIGVNVSLGRKENRLALGESLLLNVNVDRTAFVYCFYQDAKGGLTQIYPSEFQ
                     PIPRLQAQRSMLIPDAGNPDSFTLETTAPGHESAGCFASNSDLAPQLDHDLHIAPLKP
                     ITSVHSLAELDQKFHAMVTAPAHMGAGYANWEVAK"
BASE COUNT          344 a          890 c         1018 g          498 t
ORIGIN      
        1 aaaagctgcg gtcggcgcta gcggcacgcc ccgcgtgagc ccgcaccgca gacgcggccg
       61 tcggtccaga tcgcgccgct gaagtccgtg tcttccagtt cggcattggc gaggttcgca
      121 ccggtcaggt tggcgccggc aaagcgtgcg cgcgccaatc cggcgttggc gaaatcggcg
      181 ccgcgcaggt cggcgcggct gaagtcggct tcggtcagcg tggcgttggc gaacgacacg
      241 cgcaccagtg cctgaccgga gaagttggtg tttgccagct cgccgttggc gtagctgtgg
      301 acgccttttg tcaccacgcc gggcaccgaa gcggcgccac tgctggcagt ggcctgggtc
      361 gtgccatcgc cggtcaccgt ggtgacgccg ttcgaggtga cgatgcggct gttgcctgtg
      421 gtcgtcgtgg tgtgcccgtc gatcgtctgt tcggagacgg cgtcggtgcc gtgcgccgcg
      481 ttgacgatga cgccgttgac gttggcgccg tgcacgttct gccgtgatcg cgcgccaccg
      541 gacgcggcgg caccggcatc gatgtgcgcg ctgccgtcgt ggctggcgtc gatgcgggcc
      601 ttgcccgatg acgcggcctt gatatcgaca tcgccgtttg cgctgctgat ctgggtgtcg
      661 ccggcgcgga tcttcacgtc gccgccgttg acttcgacct gtgcggtcag cgtcgcggcg
      721 ggcaggccga gcaaggcgag caatgggagt cggatggtgg gcatgatggc ctccttcggt
      781 cggttggggc gaatcgcggc ctattgctgc ggtttgccgg tggacaggcg cgaacggctg
      841 cgtgcagcag cgtccgcgtc gtacaaggtg gcaccgttct tcggcgccgc cggtgcggca
      901 atgctgtcgc cgaggccgag cgtgccgggc gggatatagc gctggttgcg cacggtcgtg
      961 cccggcagcg tttccttcgc gccgggtttc aatgagggca gatcgctcaa cgggtcgtcg
     1021 tcctcgagca ggtcgccgag gcccgggcgc gtttccccgg cgtcggtgct gagctggccg
     1081 agttcgacca tccattgcgg atcgaccggc accgcctcgg tcgagcgcga ggacaccatc
     1141 gcgatcatgc gttgcgcggc gacgctgccg ccgtgcgcgg cgatgcggta gagacggatc
     1201 gcctcgccgt agttgaccgc gacgccgagg ccgcggtggt agcgcgtggc gagttcgtag
     1261 gtcgcgttcg catcgccggc ggcggacgcg gcgcgcagct cgtcgatcgg ctgcagcgcg
     1321 ggctcgccgg cctgagcctg gcgttgcacg cggttggcgt tgcgcggcgc actcgcgctg
     1381 ccggcgagtt gcgcctggcg gtaccagagc agcgcggtcg gtcgctgccc gctgcgcaga
     1441 tagccgttgg cgagcgcgtt cgcggccggg gcgtcgccgc gtgcggccgc gcgctcacgc
     1501 gcctcgcgcg cggcgacgag cgactgcggc gtggcgaggc tttcgtcgag caggctcagc
     1561 agatacgcgg cgcgcgcgta tcccgcctgc tcggcgccat tcaggtattc gcgcgcggct
     1621 tgcggattgc gcgccgcgcc gagacccttg gtgagcaggt agcctgcgcc gagcatcgcg
     1681 cgtgcgtcgc cgagctgcgc ggcgcgatcg aaatccgcga acgcggtctt gtagtcgggc
     1741 ttgccgccgc gccgtccctt gtacgcctgc cacgccgaac gcagcaggga ctgcggactg
     1801 ccgtcggtcg cattgcccgc cgcgaccgcg tcgtcggtgg gcggcggtgg tttgtttttg
     1861 aggatcacct cgcccacgcc gtcggcgcag gcgaagacga tcgcggccgc gagcgccgcc
     1921 gcccatgctc gcccgcgcgt agtccgcgta gtcacggcac gcccgatgcg cattcggcat
     1981 cgcatttcat ttcgccactt cccagttcgc atagccggcg cccatgtgcg cgggcgcggt
     2041 gaccatggcg tggaatttct ggtcgagctc ggccagcgag tgcacgctcg tgatcggctt
     2101 gagcggagcg atgtgcaaat catggtcgag ctgcggcgcg aggtcactgt tcgaagcgaa
     2161 gcagccggcg gactcgtggc cgggcgcggt cgtctcgagg gtgaagctgt ccggattgcc
     2221 ggcatcggga atcagcatcg agcgctgcgc ttgcaggcgc ggaatcggct ggaactcgct
     2281 cggatagatc tgggtcagcc cgcctttcgc gtcctgatag aagcagtaga cgaacgcggt
     2341 gcgatcgacg ttgacattga gcagcagcga ctcgccgagc gcgaggcggt tttccttgcg
     2401 tccgagcgag acgttgacgc cgatcgggtt cgcgttcgcg ctgtcgaacg cgggctggcc
     2461 gttgcgcggc ttctgcgcga agccgcggcc cttgtcgccg ttgtcgcgcg cccagccgat
     2521 gcgcacgaaa ccgttctggc tgtcgctggc gacgtagttc ttcatcagcc gctcgtaggt
     2581 ctcgaaatcg atgtcgcccg atgcggtcgc ctggtgatcg gcctggtaca gcacgatcgc
     2641 ctcgcgcagc gcgggcgagg tctgtccgtc gattgcgccc tcgtaatagc ccatgctgcg
     2701 caggccggtc tggaacaggc gcgcctgctc gtccggtgcc atgccgttga
//
LOCUS       DASRDG010000461         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_300890, whole genome shotgun sequence.
ACCESSION   DASRDG010000461 DASRDG010000000
VERSION     DASRDG010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300890"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /gene="dtd"
                     /locus_tag="VFA35_07685"
     CDS             <1..339
                     /gene="dtd"
                     /locus_tag="VFA35_07685"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZT56090.1"
                     /translation="GPAQRDRLLERLLDYRVFADDAGRMNRSLRDTGGGLLLVPQFTL
                     AADTRSGNRPGFSTAAQPAAASELFGQLVEAARAAHPVVASGEFGAYMQVSLTNDGPV
                     TFWLQVSPKA"
     gene            434..577
                     /gene="tatA"
                     /locus_tag="VFA35_07690"
                     /pseudo
     CDS             434..577
                     /gene="tatA"
                     /locus_tag="VFA35_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            707..1096
                     /gene="tatB"
                     /locus_tag="VFA35_07695"
     CDS             707..1096
                     /gene="tatB"
                     /locus_tag="VFA35_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HZT56091.1"
                     /translation="MFDIGFSEVLVIFGLALVVLGPDKLPQLARTIGRWAGRARAMAR
                     QFRDQLEAESEGIKSDLKVTLPPDFVVPATSAAPPPTASPPPSPAPTAPPPPAEPVLQ
                     AEPAPPPQGQQQLPIDHPEIGHHDRRE"
     gene            1080..1847
                     /gene="tatC"
                     /locus_tag="VFA35_07700"
     CDS             1080..1847
                     /gene="tatC"
                     /locus_tag="VFA35_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZT56092.1"
                     /translation="MTGENEPLLEGTLISHLLELRTRLMRAFLAIILVFIPCAYYSNQ
                     LFQWLSHPLLMKLPAQAVLISTSITAPFTAPLKLAFVVALVLAMPYVLYELWAFVAPG
                     LYKQEQTFALPLLISSVILFYVGVAFAYFLVLPLIFTFFVSTTPQGVQLMADISTYME
                     FVLVLLFSFVAAFEVPVAVVLLVATGLVPLEKLTRIRGYVLIAVFVIAAILTPPDAIS
                     QTVMAIPMYLLYEGGILFARLALRSRKRREGSHGSPA"
     gene            1874..>2495
                     /locus_tag="VFA35_07705"
     CDS             1874..>2495
                     /locus_tag="VFA35_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HZT56093.1"
                     /translation="MGAAPRNELTIRGLVLGVIITVAFTAANVYFGLKAGLTFATSIP
                     AAVISMAVLRGFRGMTVQENNIVQTVASAAGTLSSIIFVLPGLVMIGWWQGFPYWTTA
                     AITGTGGILGVLFSVPLRRTLIIEQKLRFPEGIAAAEVLKVGANPGVGAKVLGLATVV
                     GGLFKFITSGLRSLPEAFTIKGFIGERGIAFFGYGFSPALLGVGYIV"
BASE COUNT          371 a          863 c          795 g          466 t
ORIGIN      
        1 gggcccgccc agcgggaccg cttgctcgaa cggctgctcg actatcgagt gttcgcggac
       61 gacgccgggc gcatgaaccg ttcgctccgc gacaccggcg gcggattgct gctggtgccg
      121 caattcacgc tggcggccga cacccgctcg ggcaatcgac cgggattctc caccgctgcc
      181 cagcccgccg cggccagcga gctgttcggg cagctcgtgg aggccgcgcg cgcggcccat
      241 ccggtcgtgg ccagcggcga gttcggcgcc tacatgcagg tgtcgttgac caatgacggc
      301 ccggtcacgt tctggctcca ggtgagcccg aaagcgtgaa cctcggcact tccctgcggc
      361 tcggctgctg cctgggccac agttcgccta tcattgcgga tcggacatat ccgcctcggg
      421 cctggagggc gccatggacc tgttttcacc ccgtcacttg ctgatcatcc tgctggtcgt
      481 gctgctggtg ttcggcacca agaagctgcg caccatcggc tccgacctgg gcgcagcggt
      541 gcgcggcttc aagaagtcga tgaacgaagg cgagcaggag cagcagtccg cccagacgcc
      601 cccgcccggc ggagccaagg acgcggagtt ccacgagtct gccgggggta tcgaaccccc
      661 gcgccaggac aaccggcgca gctgagcttc gcttcgctag gcgcgcatgt tcgacatcgg
      721 cttctcggag gtcctggtga tctttggcct cgccctcgtg gtgctgggcc cggacaagct
      781 cccgcagctc gcgcgcacga tcgggcgctg ggccgggcgc gcacgcgcca tggcgcgcca
      841 gttccgcgac cagcttgaag ccgaaagcga agggatcaag agcgacctca aggtaacgtt
      901 gccgcccgat ttcgtggtgc cagcgacgtc cgccgcgccg ccaccgaccg cgtcgccgcc
      961 accgtcgccg gcaccgaccg caccgccgcc accggccgag ccagtgctgc aggccgagcc
     1021 agctccgccg ccgcaggggc agcagcagct gcccatagac catcctgaga tcgggcacca
     1081 tgacaggcga gaatgaaccc ctgctcgaag gcacgctgat ctcgcacctg ctggaactgc
     1141 gcacgcgcct gatgcgcgcg ttcctcgcca tcatcctcgt cttcataccc tgcgcctact
     1201 attcgaacca gctgttccag tggctgtcgc acccgctgct gatgaagttg ccggcgcaag
     1261 cggtgctgat ctcgaccagc attaccgcgc ccttcaccgc tccgctcaag ctcgcgtttg
     1321 tggtcgcact cgtgctggcg atgccctacg tgctctacga gctgtgggcg ttcgtggcgc
     1381 cggggctcta caagcaggag cagactttcg cgctgccgct gctcatttcc tcggtgatcc
     1441 tgttctacgt cggcgtcgcc ttcgcctact tcctggtact gccgctcatc ttcaccttct
     1501 tcgtcagcac gacaccccag ggcgtgcagc tcatggccga catctccacg tacatggaat
     1561 tcgtgctggt gctgctgttc tcattcgttg ccgcgttcga agtgccggtt gccgtcgtgc
     1621 tgctggtggc gacaggactg gtgccgctcg agaagctgac gcgcatccgc ggctacgtgc
     1681 tgatcgcggt attcgtgatc gccgcgatcc tgacgccgcc ggatgccatc tcgcagacgg
     1741 tcatggcgat tccgatgtac ctgctttacg aaggtggcat cctgttcgcc cggctggcgc
     1801 tgcgcagccg caagcgccgg gaaggcagcc acgggagtcc tgcatgagcc aagccaaggc
     1861 cacggcggcg ccggtgggcg ccgcgccgcg caacgaactc acgatccgcg gcctcgtgct
     1921 cggagtcatc atcacggtgg cgttcacggc cgcgaacgtc tacttcggcc tcaaggcagg
     1981 cctgaccttc gcgacctcga tcccggcggc cgtgatctcg atggccgtgc tgcgtggctt
     2041 ccgcggcatg acggtgcagg agaacaacat cgtgcagacc gtcgcctccg cggccggcac
     2101 gctctcatcg atcatcttcg tgctgccggg cctggtcatg atcggctggt ggcagggctt
     2161 tccctattgg acgaccgcgg cgatcaccgg cactggcggc attctcggcg tgctgttctc
     2221 ggtgccgctg cgccgaacct tgatcatcga acagaagctg cgatttccgg aaggcatcgc
     2281 ggcggccgaa gtgttgaaag tgggtgcgaa tcccggcgtc ggagccaagg ttcttgggct
     2341 cgccaccgtg gtcggcgggc tgttcaagtt catcacctcg ggattgcggt cgctgcccga
     2401 ggccttcacc atcaagggct tcatcggcga gcgcggcatc gcgtttttcg gttatggatt
     2461 ctcccccgca ttgctcggcg tcggctacat cgtcg
//
LOCUS       DASRDG010000462         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1581291, whole genome shotgun sequence.
ACCESSION   DASRDG010000462 DASRDG010000000
VERSION     DASRDG010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581291"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFA35_07710"
     CDS             complement(<1..605)
                     /locus_tag="VFA35_07710"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZT56094.1"
                     /translation="MQDPSPATHWGAEALWEQLVPLLPGLSVEVVHRLHSTNTTLLER
                     ARATVDTRTDGDAVLVRRSVESGAFGRRAVDLQPCLLVAEHQSAGRGRQGRLWQSAPG
                     ASLTFSLGLPLAATDWSGLSLAVGVALCEALAPADASLARSGPQIGLKWPNDLWLMDG
                     PVHSAAGGRKLGGILIETVAAGGVANSKRLVIIGIGLNVRP"
     gene            complement(624..1124)
                     /locus_tag="VFA35_07715"
     CDS             complement(624..1124)
                     /locus_tag="VFA35_07715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HZT56095.1"
                     /translation="MTQDSAASPPGTTSAKKAGKRIQVRKSGVHGKGVFSTVKLKKGE
                     VLIEYVGEIISWKEALRRHPHDPKDPDHTFYFHIDDKRVIDAKFGGNAARWINHACTP
                     NCEADEIDGRIFIKALKKIEPDTELFYDYGLIIDEPYTKKLKKQFECRCGTLGCRGTM
                     LAPKRR"
     gene            1253..>3387
                     /locus_tag="VFA35_07720"
     CDS             1253..>3387
                     /locus_tag="VFA35_07720"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HZT56096.1"
                     /translation="MTKSLIIAEKPSVAQDIVRALTPTAGKFDKHDEYFESDGYLVTS
                     AVGHLLEIKAPEEFDVKRGKWSFANLPVIPPHFELNPIDKSKGRLGAIVKRVKRKDVS
                     ELINACDAGREGELIFRLIQQHSKSKHPVKRLWLQSMTPAAIREGFEHLRSDQQMLPL
                     ADAARCRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIMVEREEKIRKH
                     VARDYWELKATFAAQAGEYEGKWFDPKWKKNPDDAELRADRIWNERDAKAIADAARGQ
                     PASVTEEAKPSTQSSPGLYDLTTLQREANSRLGFSAKTTLSIAQALYEKHKVLTYPRT
                     DSKHLPEDYVEVVKQTMKMISTEDMPGPLRALATHAKTAIKEGYVKPNKKIFDNAKVS
                     DHFAIIPTLLAPKSLSEIEAKLYDMVVKRFLAVFFPPAEFMVTTRISTVKAGDKEHKF
                     QTNGKVMVKPGWLAVYGREAQEDDANLVAVAPGEIVRTEDVAVNALKTKPPARYTEAT
                     LLSAMEGAGKLIDDDELREAMQEKGLGTPATRAAIIEGLIYEKYIHRDGRDLVPGAKA
                     FQLMTLLRGLDIEDLTKPELTGNWEFQLSEMEKGRLEREAFMAEIARMAERIVKKAKE
                     YDRDTIPGDYATLKAPCPNCGGVVKENYRRFTCQGKKGDGSDSCEFSITKIPAGRAFE
                     AEEVEQFLRDKKIGPLEGFRSKAGWPFTA"
BASE COUNT          621 a         1109 c         1163 g          494 t
ORIGIN      
        1 aacggccgca cgttcaggcc gatgccgatg atcacgaggc gcttcgaatt ggcaacgccg
       61 ccggcggcca cggtctcgat caggatgccg ccgagcttgc ggccgcccgc ggcgctgtgc
      121 accgggccgt ccatcagcca caggtcgttc ggccacttca ggccgatctg cgggccgctg
      181 cgcgcaaggc tcgcatcggc cggcgcgagc gcctcgcaca gcgccacgcc gaccgcgagc
      241 gacagccccg accagtcggt cgctgcgagt ggcaggccga gcgagaaggt cagcgaagcg
      301 cccggcgccg actgccacag ccggccctgc cggccgcgcc cggcgctctg gtgctcggcg
      361 accagcaggc acggctgcag gtcgaccgcg cggcgcccga acgcgccgct ctcgacgctg
      421 cggcgcacca gcacggcgtc gccgtcggtg cgcgtgtcga ctgtcgcgcg ggcgcgctcg
      481 agcagggtcg tgttggtcga gtgcagccgg tgcacgacct cgacgctcag ccccggcagc
      541 agcggcacga gctgctccca cagcgcttcg gcgccccagt gggtcgcggg agacgggtct
      601 tgcatgccag gctgccggcg tgttcagcgc cgcttcggcg cgagcattgt gccgcggcag
      661 cccaaggttc cacagcggca ctcgaactgc ttcttcagct tcttggtata cggctcgtcg
      721 atgatcaggc cgtagtcgta gaacagctcg gtgtcgggct cgatcttctt cagcgccttg
      781 atgaagatgc ggccgtcgat ctcatcggcc tcgcagttcg gcgtgcacgc gtggttgatc
      841 cagcgcgccg cgttgccgcc gaacttggcg tcgatcacgc gcttgtcgtc gatgtggaag
      901 tagaaggtgt ggtccgggtc cttcgggtcg tgcgggtggc ggcgcagcgc ctctttccag
      961 ctgatgatct cgccgacgta ctcgatcagc acctcgccct tcttgagctt gaccgtactg
     1021 aagacaccct tgccgtgcac gcccgacttg cgcacctgga tgcgcttgcc cgccttcttc
     1081 gccgaggtcg ttccgggggg gctggcggcg ctgtcttgcg tcatcggatt ctttatgtac
     1141 attgaaatca tgggtgcgcg cgcgtgtgtg cgtgtacgtg tgcacatgcg cgcgagaagc
     1201 ccggattgta ttgatgcgtg aggcccgccc cgcgcgaacc gagaagaaac acatgaccaa
     1261 gtcactgatc attgccgaga agccttccgt cgcccaggac atcgtgcgcg cgctgacgcc
     1321 gaccgccggc aagttcgaca agcacgacga atacttcgag agcgacggct acctcgtcac
     1381 ctcggccgtg ggccacctgc tcgagatcaa ggcgccggag gagttcgacg tgaagcgcgg
     1441 caagtggagc ttcgcgaacc tgccggtgat tccgccgcac ttcgagttga acccgatcga
     1501 caagagcaag gggcgcctcg gcgcgatcgt caagcgcgtc aagcgcaagg acgtgagcga
     1561 gctgatcaac gcctgcgacg cgggccgcga gggcgagctg atcttccggc tgatccagca
     1621 gcacagcaag agcaagcacc cggtcaagcg gctgtggctg caaagcatga cgccggcggc
     1681 gatccgcgaa ggcttcgagc acctgcgcag cgatcagcag atgctgccgc tcgccgacgc
     1741 ggcgcgctgc cgcagcgagg ccgactggct ggtcggcatc aacggcacgc gcgcgatgac
     1801 ggcgttcaac tcgcgcgacg gcggcttctt cctgacgacg gtgggccggg tgcagacgcc
     1861 cacactctcg atcatggtcg agcgcgagga gaagatccgc aagcacgtcg cgcgcgacta
     1921 ctgggagctg aaagcgacgt tcgcggcgca ggccggcgag tacgaaggca agtggttcga
     1981 cccgaagtgg aagaagaacc cggacgacgc ggagctgcgc gctgatcgga tctggaacga
     2041 gcgcgacgcc aaggcgatcg ccgacgccgc gcgcggccag ccggccagcg tgaccgaaga
     2101 agccaagccg agcacgcaga gttcgcccgg cctgtacgac ctgacgacgc tgcagcgcga
     2161 ggccaactcg cgcctgggct tctccgccaa gaccacgctg tcgatcgcgc aggcgctgta
     2221 cgagaagcac aaggtgctga cctacccgcg gaccgacagc aagcacctgc ccgaagacta
     2281 tgtcgaagtc gtcaagcaga cgatgaagat gatctcgacc gaggacatgc ccggcccgct
     2341 gcgggcattg gccacgcatg cgaagaccgc gatcaaggag ggctacgtca agcccaacaa
     2401 gaagatcttc gacaacgcga aggtctccga ccacttcgcg atcatcccga cgctgctcgc
     2461 gccgaagagc ctcagcgaga tcgaggccaa gctgtacgac atggtcgtca agcgcttcct
     2521 cgcggtgttt ttcccgccgg ccgagttcat ggtgacgacg cggatctcga ccgtgaaggc
     2581 gggtgacaag gagcacaagt tccagaccaa cggcaaggtg atggtcaagc cgggctggct
     2641 cgcggtctac ggccgcgagg cgcaggagga cgacgcgaac ctggtcgcgg tcgcgcccgg
     2701 cgagatcgtg cgcaccgagg acgtggcggt gaacgcgctg aagaccaagc cgccggcgcg
     2761 ctacaccgag gcgacgctgc tcagcgcgat ggaaggcgcg ggcaagctga tcgacgacga
     2821 cgagctgcgc gaggcgatgc aggagaaggg cctgggcacg ccggcgacgc gtgcggccat
     2881 catcgaaggg ttgatctacg agaagtacat ccaccgcgac ggccgcgacc tggtgccggg
     2941 cgcgaaggcg ttccagctga tgacgctgct gcgcggcctg gacatcgagg acctgaccaa
     3001 gcccgagctg accggcaact gggagttcca gttgtcggaa atggagaaag gcaggctcga
     3061 gcgcgaggcc ttcatggccg agatcgcgcg catggccgag cgcatcgtca agaaggcgaa
     3121 ggagtacgac cgcgacacca tccccggcga ctacgcgacg ctgaaggcgc cgtgcccgaa
     3181 ctgcggcggc gtcgtcaagg agaactaccg ccgcttcacc tgccagggca agaagggcga
     3241 cgggtcggac agctgcgagt tctcgatcac caagatcccg gccggccgcg cgttcgaggc
     3301 cgaggaggtc gagcagttcc tgcgcgacaa gaagatcggc ccgctcgaag gcttccgctc
     3361 caaggcgggc tggccgttca cggccga
//
LOCUS       DASRDG010000463         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1102644, whole genome shotgun sequence.
ACCESSION   DASRDG010000463 DASRDG010000000
VERSION     DASRDG010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102644"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFA35_07725"
     CDS             <1..330
                     /locus_tag="VFA35_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56097.1"
                     /translation="NTYTSGDYVGRWLWSRDGPNDDLLGHPLAEVLRPAALGRGSVYD
                     AFAPTPPEGRDLDAAAEAETCLGVGAHTHYFEPDQQEVAWLIDYLLGSRYTVSGTSRD
                     GSGGGVP"
     gene            complement(279..716)
                     /locus_tag="VFA35_07730"
     CDS             complement(279..716)
                     /locus_tag="VFA35_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56098.1"
                     /translation="MFDVRAWLASASWACLCGIASALFLTVSLVGLFVLFFGALISAM
                     LAPLLLLLPADLQWTGWQWFAIGLALWLAVVALLGALARWGYLRCRAVVFVGAARWPR
                     ASSIVVGAVAALVLLATLPGQGARYLPQGTPPPEPSRDVPLTV"
     gene            complement(791..1318)
                     /locus_tag="VFA35_07735"
     CDS             complement(791..1318)
                     /locus_tag="VFA35_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56099.1"
                     /translation="MSAVAAAGLALSACYVVPIDPRTGQPYPPTSRESAQASGYSVAP
                     AAPLIVAQPPAPTLINVRLYPLNAQANKGGLLSAQVVDNNAGHGSFMVPYLGDTLQGE
                     STRVDASYPSFGIVHQQVLGASPRTVTGRRGVANAHGAHGVNAQCEYVLTGPGSGTGV
                     CQFSDGAYYQMHFGG"
     gene            complement(1540..>2035)
                     /locus_tag="VFA35_07740"
     CDS             complement(1540..>2035)
                     /locus_tag="VFA35_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HZT56100.1"
                     /translation="FQFPYMEKGSKRPDLPAVAQAAVRAAVDEAAGLGAGLPLFAGGR
                     SFGGRMTSQAQARAPLAGVRGLVFVGFPLHPAGKPGTERAAHLTTIEIPMLFLQGTRD
                     ELAGLDLLNPVVDALGAKATLELFDDADHSFHVRARSGRNDAQVRDAMLDATVAWMAT
                     VAAR"
BASE COUNT          338 a          716 c          743 g          238 t
ORIGIN      
        1 aacacctata ccagcggcga ttacgtcggc cgctggctct ggagccgcga cggcccgaac
       61 gacgacctgc tcggccaccc gctggccgag gtgctgcgtc ccgcagcgct cggccgcggc
      121 tcggtctacg acgcgttcgc gcccaccccg ccggagggcc gcgatctcga tgctgcggcc
      181 gaggccgaga cctgcctcgg cgtcggcgcg cacacgcatt acttcgagcc cgaccagcag
      241 gaagtggcct ggctgatcga ctacctgctc ggcagccgct acacggtcag cgggacgtcg
      301 cgcgacggct cgggcggcgg cgtgccctga ggcaggtagc gtgcgccctg gcccggaagg
      361 gtcgccagca gcaccagggc ggcgacggca ccgacgacga tcgacgaggc ccgcggccag
      421 cgggccgcgc cgacgaacac gacggcgcgg caacggaggt atccccagcg ggcgagcgca
      481 ccaagcagcg cgacgaccgc cagccacagg gccaggccga tggcgaacca ctgccagccg
      541 gtccactgca agtccgcggg cagcagcagg agcagcggcg ccagcatcgc cgagatcagc
      601 gcgccgaaga agaggacgaa gagacccacg agcgagaccg tgaggaacag cgccgaggcg
      661 attccgcaaa ggcaggccca gctcgccgaa gccagccagg cgcggacatc gaacaaatgg
      721 actctccgtc acggcgcgcg gaccgtcccg cgccggcgcc gatgttatga cctgacgcgt
      781 gcgctgcgcg tcagccgccg aagtgcatct ggtagtaggc gccgtcggag aactggcaca
      841 cgccggtgcc ggagcccgga ccggtgagca cgtattcgca ctgcgcgttg acgccgtgcg
      901 caccgtgggc attggcgacg ccgcgccgtc cggtgaccgt acgcgggctg gcgccgagca
      961 cctgctggtg gacgatgccg aacgacggat agctggcatc gacccgggtc gattcgccct
     1021 gcagggtgtc gccgaggtag ggcaccatga agctgccgtg gccggcattg ttgtcgacca
     1081 cctgggccga cagcagcccg cccttgttgg cctgcgcgtt gagcggatag aggcggacgt
     1141 tgatgagcgt cggcgccggc ggctgcgcga cgatgagcgg cgcggcgggc gccaccgaat
     1201 aacccgaggc ctgggccgac tcgcgcgacg tcggcggata aggctggccg gtgcgcggat
     1261 cgatcggcac cacgtagcag gcgctgagcg cgaggcccgc ggcggcaacg gcggacaacg
     1321 atgccgccct ggcgacggca cgacgaatgg gaagcgcgtt cataggattt ccgatggctg
     1381 cggcacatgc cgcggacggc agcgtcgtcg accgcggcgc gcccggcaat cgcgttcaat
     1441 caagtagcgg atcctgccgc cgacgagcgc agcctgcgcg ccgccgcgga gggcgcatcg
     1501 ctctttcgat ggtgcgagaa ggcgctcgcc gataggcgat cagcgagctg cgaccgtcgc
     1561 catccaggcc accgtggcat cgagcatcgc atcgcgcacc tgggcatcgt tgcggccgct
     1621 gcgggcgcgg acgtgaaacg aatgatcggc gtcgtcgaac agctcgaggg tcgccttcgc
     1681 gccgagcgcg tcgaccaccg gattgagcag gtcgaggccg gcgagctcgt cgcgcgtccc
     1741 ctggaggaac agcatcggga tctcgatcgt cgtgagatgc gcggcccgct cggtccccgg
     1801 cttgcccgcc ggatggagcg ggaagcccac gaagacgagc ccgcgcacgc cggcaagcgg
     1861 cgccctcgcc tgcgcctgcg aggtcatccg gccgccgaac gaccggccgc cggcgaagag
     1921 cggcaggccg gcgccgagcc ccgccgcttc atccaccgcg gcacggaccg cggcctgcgc
     1981 gaccgccggc aggtccgggc gcttcgagcc cttctccatg tacgggaact ggaag
//
LOCUS       DASRDG010000464         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1221596, whole genome shotgun sequence.
ACCESSION   DASRDG010000464 DASRDG010000000
VERSION     DASRDG010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221596"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1285
                     /gene="glcF"
                     /locus_tag="VFA35_07745"
     CDS             23..1285
                     /gene="glcF"
                     /locus_tag="VFA35_07745"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HZT56101.1"
                     /translation="MQTKLAPEFKNTPEGLEAEAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLMKQVLEGAPVTRSTQLHLDRCLTCRNCESTCPSGVQYGALIDIVRPIV
                     DDQVERPTREKAVRWLLKEGLTSPLFAPAMKLGQALRPLVPASLQNKVPAKPPARAHQ
                     WPTRTHPRKVLMLMGCVQPAMMPNINSATARVLDAAGIQTVVADEAGCCGAVRMHLDD
                     AEGGLKEMRRNIDAWWPLVSGGPGSLEGVETIVMNASGCGVTVKDYGHALARDPAYAD
                     KAARISAMTRDLSELLPELVPKLKHKVRLRGAGNRAATHLAFHPPCTLQHGQQLRGGV
                     ETHLAELGFEVRVAPVEAHLCCGSAGTYSVLQPELAYQLRDRKLAALAPLDAQTIVSA
                     NIGCIQHLQSGTATPVKHWVEVLDEALG"
     gene            complement(1311..2024)
                     /locus_tag="VFA35_07750"
     CDS             complement(1311..2024)
                     /locus_tag="VFA35_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56102.1"
                     /translation="MRTSLFRRREVWLPTVWGALLLLALGAALLFGLARAAAPWLAPH
                     APAPGARTLVVEGWLDLAEIRQAAQVARAGRYARVLTTGGPIDPLVDAAGHRSFALRA
                     AALLRANGLTELPVIAVPAPASAQERSYLSAVVLREWAKRNDPALAAFDLYSAGVHAR
                     RSWLVYRMAFGARVEVGILAARPTDYDPQRWWASSIGAKATLGEAIGLAWTTCCFWPG
                     PPGSHDERWGVPAAAGTVP"
     gene            2109..>2486
                     /locus_tag="VFA35_07755"
     CDS             2109..>2486
                     /locus_tag="VFA35_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT56103.1"
                     /translation="MGIFLIKRFATFVVTLLAASVLVFAVQGLLPGNAAQMMLGESAT
                     PEAVEVLSRRLGLDQPAAVRYGRWVAGLVTGDLGTSVAYDAPVAQLIGERLAVTAPLA
                     LLAMALTTALALALGLYAASHHGR"
BASE COUNT          353 a          922 c          895 g          316 t
ORIGIN      
        1 gtctctgatc caaatccttc cgatgcaaac caaactcgcc cccgagttca agaacacgcc
       61 cgaaggcctg gaggccgagg cgatcctgcg caagtgcgtg cactgcggct tctgcaccgc
      121 cacctgcccg acctaccagc tcctcggcga cgagctcgac ggcccgcgcg gccgcatcta
      181 cctgatgaag caggtgctcg aaggtgcgcc ggtgacgcgc agcacgcagc tgcacctgga
      241 ccgctgcctg acctgccgca actgcgagag cacctgcccg tcgggcgtgc agtacggcgc
      301 gctgatcgac atcgtgcgcc cgatcgtcga cgaccaggtc gagcggccga cgcgcgagaa
      361 ggcggtgcgc tggctgctga aagagggcct gacctcgccg ctgttcgcgc ccgcgatgaa
      421 gctcggccag gcgctgcggc cgctggtgcc ggcgtcgctg cagaacaagg tgccggccaa
      481 gccgcccgcg cgcgcgcacc agtggccgac gcgcacccac ccgcgcaagg tgctgatgct
      541 gatgggctgc gtgcagccgg cgatgatgcc caacatcaac agcgcgaccg cgcgcgtgct
      601 cgacgccgcg ggcatccaga ccgtcgtcgc cgacgaggcc ggctgctgcg gcgcggtccg
      661 catgcacctg gacgacgccg agggcgggct caaggaaatg cgccgcaaca tcgatgcctg
      721 gtggccgctg gtctcgggcg ggccgggctc gctcgaaggc gtcgagacga tcgtgatgaa
      781 cgcctcgggc tgcggcgtca ccgtgaagga ctacggccac gcgctcgcgc gcgacccggc
      841 ctacgccgac aaggccgcgc gcatcagcgc gatgacgcgc gacctgagcg agctcttgcc
      901 cgagctggtg ccgaagctga agcacaaggt ccgcctgcgc ggtgccggca accgcgccgc
      961 gacgcacctc gcgttccacc cgccctgcac gctgcagcac ggccagcagc tgcgcggcgg
     1021 cgtcgagacg cacctggcgg agctcggctt cgaggtgcgc gtcgcgccgg tcgaggcgca
     1081 tctgtgctgc ggctcggccg gcacctactc ggtgctgcag cccgagctcg cctaccagtt
     1141 gcgcgaccgc aagctcgcgg cgctggcgcc gctcgacgcg cagaccatcg tctcggcgaa
     1201 catcggctgc atccagcacc tgcaaagcgg caccgcgacg ccggtgaagc actgggtcga
     1261 ggtgctcgac gaagcgctgg gctgaacgcc ggcgccgcgc accgccccct ctacggaaca
     1321 gtgccagccg ccgccggcac gccccagcgt tcgtcgtgcg agcccggtgg cccgggccag
     1381 aagcagcacg tcgtccacgc caggccgatc gcctcgccga gcgtggcctt cgcgccgatg
     1441 ctgctggccc accagcgctg cgggtcgtag tcggtcgggc gcgcggccag aatgccgacc
     1501 tcgacgcggg cgccgaacgc catccggtac accagccacg agcggcgcgc gtgcacgccg
     1561 gctgagtaga ggtcgaaggc ggcgagcgcc gggtcgttgc gcttcgccca ctcgcgcagc
     1621 accacggcgc tgaggtagct gcgctcctgc gccgacgccg gcgcaggcac cgcgatcacc
     1681 ggcagctcgg tcagcccgtt cgcgcgcagc agcgcggcgg cgcgcagcgc aaagctgcgg
     1741 tggccggcgg cgtcgaccag cgggtcgatc gggccgccgg tcgtcagcac gcgcgcgtag
     1801 cggccggcgc gcgccacctg cgccgcctgg cggatctcgg cgaggtcgag ccagccttcg
     1861 acgaccagcg tgcgcgcgcc gggcgccggt gcgtgcggcg ccagccacgg cgcggccgcc
     1921 cgcgccagcc cgaacagcag cgcggcgccg agcgcgagca gcagcagcgc gccccagacc
     1981 gtgggcagcc agacctcgcg gcggcggaac agcgacgttc tcaaattgcg gagatgacga
     2041 atcgggtgcc tgccacgccg gcctcctgtg cgcgagcgcc tgtcgggttg tgggccatga
     2101 tattggcaat gggcatcttc ctgatcaagc gcttcgcgac cttcgtcgtc accttgctgg
     2161 cggcgtcggt gctggttttc gcggtgcagg gcctgctgcc gggcaacgcg gcgcagatga
     2221 tgctgggcga atcggccacg cccgaggcgg tcgaggtgct gtcgcgccgg ctcgggctcg
     2281 accagccggc cgcggttcgc tacggccgct gggtcgcggg cttggtcacc ggcgacctgg
     2341 gcaccagcgt cgcctacgac gcgccggtcg cgcagctgat cggcgagcgc ctcgcggtga
     2401 ccgcgccgct cgcgctgctg gcgatggcgc tgacgaccgc gctcgcgctg gcgctgggcc
     2461 tgtacgcggc ctcgcaccac ggccgg
//
LOCUS       DASRDG010000465         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1222205, whole genome shotgun sequence.
ACCESSION   DASRDG010000465 DASRDG010000000
VERSION     DASRDG010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222205"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VFA35_07760"
     CDS             complement(<1..460)
                     /locus_tag="VFA35_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56104.1"
                     /translation="MALTHASAGAAPPAALLDATSSEPRAFGYLVGDVVARTVTVHVP
                     DGLALDESSVPRPGARGKALELRSVVRDSGAESGGRRLELRLEYQVFLAPPQTRTLEM
                     PPFTLRFQGRPRAQDLRIDAWPVTVAPLVPEDVSPRRGLGELQPDAPPPLI"
     gene            complement(508..1353)
                     /locus_tag="VFA35_07765"
     CDS             complement(508..1353)
                     /locus_tag="VFA35_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZT56105.1"
                     /translation="MPAVQELHYRVGAPALGHFPGHHRSTRGDSGFELRGHAPLLEVP
                     DARRLDLNASLRDPFGNWIVRVYSQRKALAVMMVADLSASMGFVGRRRKLDVLAAFVD
                     SLAWSASRIGDSFGFVGCDTAVREDLLLPPTRSRGAGAALARTLRALHLEGHSARALS
                     IAHRHLPRQRALVFLVSDFHLPLPEIHAVTASLAHHDLVPVVIWDQLEFTLAEQHGLV
                     HVIDPESGRRRLIWWRPALRAQWESALRRRRDALLDLFRARWLKPLFVEGDFDADAVT
                     RHFYT"
     gene            complement(1403..>2278)
                     /locus_tag="VFA35_07770"
     CDS             complement(1403..>2278)
                     /locus_tag="VFA35_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZT56106.1"
                     /translation="GDVGVGKTTLLRAFARVLGGAYARTEGTIDLMPNDLVYYTYISA
                     EGKPTVEHGPLVRHGESLAIFFFNEINRARPQVHSLLLRVMAERSVSAFNREYRFPHL
                     LVFADRNRVEREETFEISSAARDRFMMEVTIDAPVDDADRRSLMFEPRFHDADRLVGD
                     LAAAMVAYQELNQVSELIQRRVQGSEALQNYALDLWRATAAPAQYGVLLPDLDTAQLM
                     LAGASPRGMSLLLRAARVTAWLDGRDYVTPEDVQSIFVETIAHRLCYQPVYEMRRHEI
                     SGRLMSGILASVAAP"
BASE COUNT          387 a          785 c          799 g          307 t
ORIGIN      
        1 cgatcagcgg cggcggcgca tcgggttgca gttcaccgag cccgcgccgc ggcgagacgt
       61 cctccggcac gagcggcgcc accgtcaccg gccaggcatc gatgcgcagg tcctgggcgc
      121 gcggccggcc ctggaagcgc agcgtgaacg ggggcatctc cagcgtgcgc gtctgcggcg
      181 gcgcgaggaa cacctggtat tcgagcctga gttcgagacg ccggccaccg gattcggcgc
      241 cactgtcgcg caccacgctg cgcaactcga gcgccttgcc gcgcgcgccc ggcctcggca
      301 cgctggactc gtcaagcgcg agaccgtccg ggacgtggac cgtgaccgtg cgcgccacca
      361 cgtcgcccac caggtaaccg aaggcgcggg gctcgctgct cgtggcatcg agcagcgcgg
      421 ccggtggcgc cgcgccggcg ctcgcatggg tcagcgccac cagcaagatt gtgcagcgcc
      481 agcggcccgc tccgaccggg gcgtggctca tgtgtagaaa tgtcgcgtca ccgcgtctgc
      541 gtcgaagtcg ccttcgacga acagcggctt gagccaccgt gcgcgaaaca ggtcgaggag
      601 cgcgtcacgg cggcgccgca gggcggactc ccactgcgca cgcagggccg gacgccacca
      661 gataagccgc cgtcgaccgc tttccgggtc gatgacatgg accaggccat gctgctcggc
      721 cagcgtgaac tcgagctgat cccagatcac caccggcacc aggtcgtgat gcgccaacga
      781 cgcggtcacg gcgtgtatct ccgggagcgg cagatgaaag tccgacacca ggaacaccag
      841 cgctcgctgc cgagggagat ggcgatgcgc gatcgacagt gcgcgtgccg aatgcccctc
      901 gaggtgcaag gcgcgcagcg ttcgggcgag cgccgcgccg gcgccgcgcg agcgggtcgg
      961 cggcagcagc agatcttcac gcaccgcggt gtcgcagccg acgaagccga agctgtcgcc
     1021 gatgcgcgag gccgaccagg ccaggctgtc gacgaacgcg gccaacacgt cgagcttgcg
     1081 acgacggccc acgaatccca tcgacgccga caggtctgcc accatcatca ccgcgagcgc
     1141 cttgcgctgg ctgtagacac gcacgatcca gttgccgaac gggtcgcgca gactagcgtt
     1201 caggtcgagg cggcgcgcat cgggcacctc gagcagcggc gcatggccac ggagctcgaa
     1261 gccgctgtcg ccgcgcgtgc tccggtgatg gcccggaaag tggcccagcg ccggtgcgcc
     1321 gacgcgatag tgcaactcct gcacggccgg caccggcgct actccgacat cctcggggac
     1381 agcaaaccag ggcctgccgc gatcatggcg ccgccacgct ggccaggatc ccgctcatca
     1441 gccggcccga gatctcatgg cggcgcatct cgtacaccgg ctggtagcac aagcgatggg
     1501 cgatggtctc gacgaagatc gactgcacgt cttcgggcgt gacgtagtcg cgcccgtcga
     1561 gccaggccgt gacccgggca gcgcgcagca gcaggctcat gccgcgcggg ctggcaccgg
     1621 cgagcatcaa ctgggccgtg tcgaggtccg gaaggagaac accgtattgg gccggcgcgg
     1681 ccgtcgcgcg ccaaaggtcc aaggcatagt tctgcaacgc ttcggagccc tgaacgcggc
     1741 gctggatcag ctccgacacc tggttcagct cctggtaggc aaccatcgcc gcggccaggt
     1801 cgccgaccag ccgatcggcg tcgtgaaagc ggggctcgaa catcaggctg cgccggtcgg
     1861 cgtcgtcgac gggcgcgtcg atggtcactt ccatcatgaa gcggtcgcgt gccgccgacg
     1921 agatctcgaa ggtctcttcg cgctcgaccc gattgcggtc ggcgaacacg agcaggtgcg
     1981 ggaagcggta ctcgcgattg aaggccgaga cgctgcgctc ggccatcacc cgcaagagca
     2041 acgaatgcac ctgcggacgg gctcgattga tctcgttgaa aaagaagatc gcgaggctct
     2101 cgccgtgccg gaccaggggc ccgtgctcga ccgtcggctt gccttcggcg ctgatgtacg
     2161 tgtagtagac gaggtcgttg ggcatcaggt cgatcgtgcc ctcggtgcgc gcataggcgc
     2221 cgccgaggac gcgggcgaag gcgcgcagca gcgtcgtctt gccgacgccg acatcgcc
//
LOCUS       DASRDG010000466         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_995845, whole genome shotgun sequence.
ACCESSION   DASRDG010000466 DASRDG010000000
VERSION     DASRDG010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995845"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VFA35_07775"
     CDS             complement(<1..1048)
                     /locus_tag="VFA35_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZT56107.1"
                     /translation="MSTHAPTIKLRDPPPRAVWALEQAGVPPLLARLFAGRGVRTHDE
                     LDDGLARLLPPSELLGVQAAARLLADALHARRRVCVVADYDCDGATACAVALRGLALL
                     GAAPETLHYVVPDRAVHGYGLTPAIVELARAKVPGGPAVLLTVDNGIASLQGVAHARA
                     LGIDVVVTDHHLPALVDAAAVLPDANVIVNPNQPGCGFGSKNLAGVGVIFYVLLALRA
                     ELRERGVFTAANQPRLDALLDLVALGTVADVVKLDANNRRLVAQGLRRIRAGQMQPGV
                     AALFAAAARDARRASGFDFGFALGPRINAAGRLSDMTLGIECLLTDDPARAAELAKLL
                     DTINRERREVESGMR"
     gene            complement(1048..1989)
                     /locus_tag="VFA35_07780"
     CDS             complement(1048..1989)
                     /locus_tag="VFA35_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56108.1"
                     /translation="MHLLIPFASALSDAASHVLRDLALPNLSALLARLTLAQRDDADA
                     YTLSPPHERALAAAAGWQGADGCLPFAAQAAAHDGLEAGDLAWGLVTPSHWHVGRDHV
                     TLADPAALQLSEAESRAAFDAVRELFESEGFRLEWGAPMRWYAAHESLVELPCASLDR
                     VIGRNVELWMRGSEARSRPAKLVRRLQSELQLLLYPHALNDEREQRGALTLNSFWLSG
                     CGRAQREEGTPPRVDASLRAPLLAEDWAGWAEAWRAVDAGPIAELLAAARAGDAVALT
                     LCGDRGAHRFESVAQSMWQRLGSRFKSPEPHAVLEAL"
     gene            2060..>2248
                     /locus_tag="VFA35_07785"
     CDS             2060..>2248
                     /locus_tag="VFA35_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZT56109.1"
                     /translation="MKSADKRFDVAIVGGGLAGVVALAYARRAGLDAVVLERQARVGG
                     LWRDLPAWQDIQISAADWT"
BASE COUNT          305 a          806 c          856 g          281 t
ORIGIN      
        1 cgcgcatgcc cgactcgacc tcgcggcgct cgcggttgat cgtgtcgagc agcttggcca
       61 gttcggcggc gcgcgccggg tcgtcggtca gcaggcactc gatgccgagc gtcatgtccg
      121 acagccgccc cgcggcgttg atgcgcggcc ccagcgcaaa gccgaagtcg aagccgctcg
      181 cgcggcgcgc gtcgcgcgcg gcggccgcga acagcgcggc cacgcccggc tgcatctgcc
      241 cggcgcggat gcggcgcagg ccctgcgcga cgaggcggcg gttgttggcg tcgagtttga
      301 ccacgtcggc gacggtgccg agcgcgacca ggtcgagcag cgcatcgagc cgcggttgat
      361 tcgctgcggt gaacacaccg cgctcgcgca gctcggcgcg cagcgcgagc agcacgtaga
      421 agatcacgcc gacgccggcg aggttcttgc tgccgaagcc gcaacccggc tggttcgggt
      481 tcacgatcac gttcgcgtcg ggcagcacgg cggcggcgtc gacgagtgcc ggcaggtggt
      541 ggtcggtcac gaccacgtcg atgccgagcg cgcgcgcatg cgccacgccc tgcaggctcg
      601 cgatgccgtt gtcgaccgtc agcagcacgg cggggccgcc cggcaccttc gcgcgcgcga
      661 gctcgacgat cgccggcgtc aggccgtagc catggaccgc gcgatcgggc acgacgtagt
      721 gcagcgtctc gggcgccgcg ccgagcagcg ccaggccgcg cagcgcgacg gcgcaggcgg
      781 tcgcgccgtc gcagtcgtag tcggcgacga cgcagacacg gcgcctcgcg tgcagcgcat
      841 cggcgagcaa gcgcgccgcg gcctgcacgc cgagcagctc cgacggcggc agcaggcgcg
      901 ccaggccatc atcgagctcg tcgtgcgtgc gcacgccgcg gccggcgaac aggcgcgcca
      961 gcagcggcgg cacgcccgcc tgttcgagcg cccagacggc gcgcggcggc gggtcgcgca
     1021 gcttgatcgt tggtgcgtga gtgctcatca gagtgcttcc agcaccgcat gcggttcggg
     1081 cgacttgaag cggctgccga ggcgttgcca catcgattgc gccacgctct cgaagcgatg
     1141 tgcgccgcgg tcgccgcaca aggtcaaggc gaccgcatcg ccggcgcgcg cggcggcgag
     1201 cagctcggcg atcggcccgg catcgaccgc gcgccaggct tcggcccaac cggcccaatc
     1261 ctcggccagc agcggcgcgc gcagcgaggc atcgacgcgc ggcggcgtgc cctcttcgcg
     1321 ctgcgcgcgg ccgcagccgc tgagccagaa cgagttcagc gtgagcgcgc cgcgctgctc
     1381 gcgctcgtcg ttcagcgcgt gcgggtagag cagcagctgc aactcgctct gcagccgccg
     1441 caccagcttg gccgggcggc tgcgcgcctc gctgccgcgc atccagagct cgacgttgcg
     1501 gccgatcacg cggtcgagcg acgcgcaggg cagctcgacc aggctttcgt gcgccgcgta
     1561 ccagcgcatc ggtgcgcccc attcgaggcg gaagccctcg ctctcgaaca gctcgcgcac
     1621 cgcgtcgaag gcggcgcgcg attcggcctc gctgagctgc agcgcggccg ggtcggccag
     1681 cgtcacgtgg tcgcggccga catgccagtg gctcggcgtg accaggcccc aggccagatc
     1741 gccggcctcc agcccatcgt gcgcggccgc ctgcgccgcg aacggcaggc aaccgtcggc
     1801 gccctgccag ccggccgcgg cggcgagcgc gcgttcgtgc ggcggcgaga gcgtgtaggc
     1861 gtcggcatcg tcgcgctgcg cgagcgtcag ccgcgcgagc agcgcagaca ggttcggcag
     1921 cgcgaggtcg cgcagcacat gcgacgcggc gtcggagagc gccgacgcga aggggatcag
     1981 caggtgcatg gggccgatta tcgcgggcag ggtgcatgag tcgttgctca ggcggcccaa
     2041 gagggggtta gcatgtccca tgaaaagtgc cgacaagcgg ttcgacgtgg ccatcgtcgg
     2101 cggcggcctg gcgggtgtcg tggcgctggc ctatgcgcgc cgggccgggc tcgacgccgt
     2161 cgtgctcgag cggcaggccc gtgtcggcgg gctgtggcgt gacctcccgg cgtggcagga
     2221 catccagatc agcgccgccg actggacg
//
LOCUS       DASRDG010000467         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_183929, whole genome shotgun sequence.
ACCESSION   DASRDG010000467 DASRDG010000000
VERSION     DASRDG010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183929"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="VFA35_07790"
     CDS             <1..2147
                     /locus_tag="VFA35_07790"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZT56110.1"
                     /translation="IVEDPLEAGNLTARSNLVAVITNGTAVLGLGNIGPLASKPVMEG
                     KGCLFKKFAGIDVFDIELAERDPDKLVDIIAALEPTLGGVNLEDIKAPECFYIETKLR
                     ERMKIPVFHDDQHGTAIISAAALLNGLELVGKKIGEVKLAVSGAGAAAIACLDLMVEL
                     GVARANIFVCDSKGVVHDRREDKLDASKQRYAQKTPARTLADVMVGADVLLGCSAAGA
                     VTQAMVASMARQPIILALANPEPEIRPELAKAVRPDCIIATGRSDYPNQVNNVLCFPY
                     IFRGALDSGATSITDAMKIACVREIADLAKAEISAEAAAAYAGRELRFGPDYLIPTPF
                     DSRLILRIAPAVAAAAAASGVATRPIADMEAYRQSLMRFVTHTGMFMRPVFSAAKAAP
                     ARIAYAEGEDERVLRAVQIALDEKLAKPILIGRPAVIAARIERAGLRLQPGRDVEVVN
                     PEDDARFRHYWETYHQRNARNGVTPEMAKSVVRRSNTAIAALMVHLGDADGMLCGLVG
                     RFDDHLARIDEVIGKRPQAAVYATLNALMLPKHTLFVTDAYVNENPSAEELADIAAMS
                     ADAIRHFGLEPRVAFLSHSSYGSSKRPGAVKMRRARDLFVARMPDVPADGEMQGDAAL
                     DDTLRNALMPDTTLAGTANLLVLPNLDAANILYNVLKMTGGQGVTVGPILLGCNAPAH
                     ILTPAATVRRVVNMTALAVAHAGALAAAGAAR"
BASE COUNT          378 a          853 c          770 g          308 t
ORIGIN      
        1 ccatcgtcga agacccgctc gaggccggca acctgaccgc gcgcagcaac ctggtcgccg
       61 tcatcaccaa cggcaccgcg gtgctcggcc tcggcaacat cggcccgctc gcgagcaagc
      121 cggtgatgga aggcaagggc tgcctgttca agaagttcgc cggcatcgac gtgttcgaca
      181 tcgagctcgc cgagcgcgac ccggacaagc tcgtcgacat catcgccgcg ctcgaaccca
      241 cgctcggcgg cgtcaatctc gaggacatca aggcgccgga gtgcttctac atcgagacca
      301 agctgcgcga gcgcatgaag atcccggtct tccacgacga tcagcacggc accgcgatca
      361 tctcggcggc tgccttgctg aacggcctcg aactcgtcgg caagaagatc ggcgaggtca
      421 agctcgcggt ctcgggtgcc ggcgccgcgg cgatcgcctg cctcgacctg atggtcgagc
      481 tcggcgtcgc gcgcgcgaac atcttcgtct gcgactccaa aggcgtggtc cacgaccggc
      541 gcgaagacaa gctcgacgcc tcgaagcagc gctacgcgca gaagaccccg gcacgcacgc
      601 tcgccgacgt gatggtcggc gccgacgtgc tgctcggctg ctcggccgcc ggcgcggtca
      661 cgcaggccat ggtggcgagc atggcgcgcc agccgatcat cctcgcgctc gcgaacccgg
      721 agcccgagat ccgccccgag ctcgcgaagg ccgtgcggcc cgactgcatc atcgcgaccg
      781 gccgctcgga ctacccgaac caggtcaaca acgtcctgtg cttcccgtac atcttccgcg
      841 gcgcgctcga cagcggcgcg accagcatca ccgacgcgat gaagatcgcc tgcgtgcgcg
      901 agatcgccga cctcgccaag gccgagatca gcgccgaagc cgccgctgcc tacgccgggc
      961 gcgagctgcg cttcggtccc gactacctga tcccgacacc gttcgattcg cgcctgatcc
     1021 tgcgcatcgc accggcggtg gcggccgccg ccgcggcctc gggcgtcgcg acccggccga
     1081 tcgccgacat ggaggcctac cgccagtcgc tgatgcgctt cgtcacgcac accggcatgt
     1141 tcatgcggcc ggtgttcagc gccgcgaagg ccgcgccggc gcgcatcgcg tatgccgaag
     1201 gcgaggacga gcgcgtgctg cgcgcggtgc agatcgcgct cgacgagaag ctcgcgaagc
     1261 cgatcctgat cggccggccg gccgtgatcg cggcgcgcat cgagcgcgcc gggctgcgcc
     1321 tgcagcccgg ccgcgacgtc gaggtcgtga accccgaaga cgacgcccgc ttccgccact
     1381 actgggagac ctaccaccag cgcaacgccc gcaacggcgt gacccccgag atggccaagt
     1441 cggtggtgcg gcgctcgaac acggcgatcg ccgcgctgat ggtgcatctc ggcgatgccg
     1501 acggcatgct ctgcggcctg gtcggccgct tcgacgacca cctcgcgcgc atcgacgagg
     1561 tgatcggcaa acggccgcag gcggccgtct acgcgacgct gaacgcgctg atgctgccca
     1621 agcacacgct gttcgtcacc gacgcctatg tcaacgagaa cccgagcgcc gaagagctcg
     1681 ccgacatcgc cgcgatgtcg gccgacgcga ttcgccactt cggcctggag ccgcgcgtcg
     1741 cgtttctgtc gcactcgtcg tacggcagca gcaagcggcc gggcgcggtc aagatgcggc
     1801 gcgcacgcga cctgttcgtg gcgcgcatgc ccgatgtgcc ggccgacggc gagatgcagg
     1861 gcgacgccgc gctcgacgac acgctgcgca acgcgctgat gcccgacacc acgctcgccg
     1921 gcaccgccaa cctgctggtg ctgcccaacc tcgacgccgc gaacatcctc tacaacgtgt
     1981 tgaagatgac cggcgggcaa ggcgtgacgg tcgggccgat cctgctcggc tgcaacgcgc
     2041 cggcgcacat cctgacgccg gcagcgacgg tgcggcgcgt cgtcaacatg acggcgctgg
     2101 cggtcgcgca cgccggggcg ctggcggctg ccggcgcggc acgctagctt cagccgacgc
     2161 gcacgagaaa gcgctgcagc gcctgcgcga acagcgcggg ctgttcgaca gcgctcaggt
     2221 gcgcggccgc atcgatcacc gcgagttcgg cacccgggat gcgctcgacg atcacctgcg
     2281 ccatcgccgg cggggtgccg acatcgagc
//
LOCUS       DASRDG010000468         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1676149, whole genome shotgun sequence.
ACCESSION   DASRDG010000468 DASRDG010000000
VERSION     DASRDG010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676149"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFA35_07795"
     CDS             complement(<1..261)
                     /locus_tag="VFA35_07795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HZT56111.1"
                     /translation="MDAATETLRPALRAAAAQLALDLADAQVDALLAYLALLQRWNAH
                     YNLTAVRDPAQMLTQHLADCLAVIAPLRRETSAAPVRLLDVGS"
     gene            complement(261..2231)
                     /gene="mnmG"
                     /locus_tag="VFA35_07800"
     CDS             complement(261..2231)
                     /gene="mnmG"
                     /locus_tag="VFA35_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZT56112.1"
                     /translation="MLFPKEFDVIVVGGGHAGTEAALAAARMGAATLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAATDEAGIQFRILNGSKGPAVRATRAQADRI
                     LYKAAIRQRIENQPNLWLFQGACDDLMLDGDRIAGAVTQVGIRFKARAVVLTAGTFLD
                     GKIHVGLQSHSAGRAGDPPAVALSARLKELKLPQGRLKTGTPPRLDGRSIDFSVLTEQ
                     PGDLDPVPVFSFMGDAAQHPRQVPCWITHTNERTHEIIRSGFDRSPMFTGLIDGIGPR
                     YCPSIEDKVNRFADKDSHQIFLEPEGLTTHEFYPNGISTSLPFDVQLAAVRSMVGLED
                     AYILRPGYAIEYDYFDPRALKASFETRSIGGLFFAGQINGTTGYEEAAAQGLFAGINA
                     ALQVRGGEPWLPRRDQAYLGVLVDDLITKGVTEPYRMFTSRAEFRLQLREDNADMRLT
                     EAGRAMGLVDDARWAAFNRKRDAVAREMERMRSTWVHAAALPAADATRLLGAPIEHEY
                     SLAELLKRPDVDFDGVAAVAAIARPDVAVSRETLHAELGAALADAVIDQTQISIKYAG
                     YIDKQNRDVERAASYESLRLPDELDYAQVTALSFEARQKLSKHRPQTLGQASRLSGIT
                     PAAISLLLVHLKKGRFKGFATLPADDTATTAH"
BASE COUNT          354 a          788 c          809 g          401 t
ORIGIN      
        1 gctgccgaca tccagcagcc ggaccggcgc agcgctggtc tcgcgccgaa gcggcgcgat
       61 caccgccaag caatcggcca gatgctgggt cagcatctgc gccgggtcac gcactgccgt
      121 caggttgtag tgcgcgttcc agcgctgcag cagggcgagg taggcgagca gggcgtcgac
      181 ctgcgcgtcg gcgaggtcga gcgcgagttg cgcagctgcg gcccgcagcg ccggccgcag
      241 cgtttctgtt gccgcgtcca tcagtgcgcc gtcgtcgccg tatcgtcggc gggcagggtc
      301 gcaaagcctt tgaagcggcc ctttttcaga tgcaccaaca gcagcgagat cgcggccggc
      361 gtgatgcccg acaagcgcga cgcttgacca agggtttgcg gccgatgctt gctcagcttc
      421 tgccgcgcct cgaaggagag ggccgtcacc tgcgcatagt cgagctcgtc cggcaggcgc
      481 agcgactcgt acgacgcggc ccgctcgaca tcgcggttct gcttgtcgat gtagccggcg
      541 tacttgatgc tgatctgggt ttgatcgatc accgcgtcgg cgagcgccgc acccaactcg
      601 gcatgcagtg tttcacgtga aaccgcgacg tcggggcggg cgatcgcggc caccgcggcg
      661 acgccgtcga aatccacatc gggccgcttc agcaactcgg ctaggctgta ctcatgctcg
      721 atcggcgcgc cgagcagccg ggtcgcgtca gcggcgggca gggcggccgc gtgcacccag
      781 gtcgagcgca tgcgctccat ctcgcgcgcg accgcgtcgc gcttgcggtt gaacgcagcc
      841 caacgcgcgt cgtcgaccag ccccatcgcg cggccggcct cggtcagccg catgtcggcg
      901 ttgtcttcgc gcagttgcag gcggaactcg gcgcggctcg tgaacatgcg gtacggctcg
      961 gtcacgccct tggtgatcag gtcgtcgacc agcacgccga ggtaagcctg gtcgcggcgc
     1021 ggtagccagg gctcgccgcc gcgcacctgc agcgccgcgt tgatgccggc gaacaggcct
     1081 tgcgcggcgg cttcttcgta gccggtcgtg ccgttgatct ggccggcgaa gaacaggccg
     1141 ccgatcgaac gcgtctcgaa gctggccttc agcgcgcgtg ggtcgaagta gtcgtattcg
     1201 atcgcgtagc cgggccgcag gatatacgca tcctccagcc cgaccatgct gcgcacggcg
     1261 gcgagttgca cgtcgaaggg cagcgaggtc gagatgccgt tcggatagaa ctcgtgcgtc
     1321 gtcaagccct cgggttcgag aaagatctgg tgcgaatcct tgtcggcgaa ccgattcacc
     1381 ttgtcttcga tgctcgggca gtagcgcggc ccgataccgt cgatcaagcc ggtgaacatc
     1441 gggctgcggt cgaaacccga gcggatgatc tcgtgcgtgc gttcgttggt gtgcgtgatc
     1501 cagcacggca cctgacgcgg gtgctgcgcg gcgtcgccca tgaagctgaa caccgggacc
     1561 gggtcgaggt cgccgggctg ctccgtcagc acgctgaagt cgatcgagcg gccgtccagg
     1621 cgcggcggcg tgccggtctt cagccggccc tgcggcagct tcaactcctt caggcgtgcc
     1681 gacagcgcca cggcgggcgg gtcgcccgcg cgcccggcgc tgtggctttg cagcccgacg
     1741 tggatcttgc cgtccagaaa ggtgccggcc gtcaagacca cggcgcgcgc cttgaagcga
     1801 atacccactt gcgtcacggc gccagcgatc cggtcgccgt cgagcatcag gtcatcgcac
     1861 gcaccctgga acagccacag gttcggctgg ttctcgatgc gctggcggat cgccgccttg
     1921 tacagaatcc ggtcggcttg cgcacgcgtt gcgcgcacgg cagggccctt ggaaccgttc
     1981 aggatgcgga actggatgcc cgcttcgtcg gtcgccgcgg ccatcgcgcc gccgagcgcg
     2041 tcgacctctt tcacgaggtg ccccttgccg atcccgccga tcgacgggtt gcagctcatc
     2101 tggccgagcg tctcgatgtt gtgcgtgagc agcagcgtgg ccgcgcccat gcgcgcggcg
     2161 gccagcgcgg cctcggtgcc ggcgtggccg ccgccgacca cgatcacgtc gaattccttc
     2221 gggaacaaca tcgagctctg cctgtgagtg cctatttttt gggcaacatc acattgtaga
     2281 gaatcagcgc gccagcatct cgatatgggg gatcccgtcg tcgatgtacg gcgccccgac
     2341 cgtggcgaag cc
//
LOCUS       DASRDG010000469         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_431242, whole genome shotgun sequence.
ACCESSION   DASRDG010000469 DASRDG010000000
VERSION     DASRDG010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431242"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..708
                     /locus_tag="VFA35_07805"
     CDS             109..708
                     /locus_tag="VFA35_07805"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HZT56113.1"
                     /translation="MSAPPERFDLREIAPQPWQNGAGLTREIALGGADPARFDWRLSV
                     AEVTRDAPFSAFPGVDRCIVLLAGAGKRLRSPDGAIDHALTRPLEPFRFPGEVPLAAT
                     LAGGASRDFNVMMRRGVFRSELRCTHAAADLAGGDVSFALCIAGAWALAEDATSATTA
                     PIEPLQALLWRAPVAPLRLEPRGAAATLLLVRLYHDRPR"
     gene            705..2090
                     /locus_tag="VFA35_07810"
     CDS             705..2090
                     /locus_tag="VFA35_07810"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HZT56114.1"
                     /translation="MNTARLFAEHALLPTGWARDVLLVWDDAGVLTEVTAGAPAPPGA
                     QRALGPLLPGMPNLHSHAFQRAFAGLSEWRSSAEADSFWTWRDLMYRIANAVSPDQLE
                     AIATRLYVEMLAAGYTTVCEFHYLHHDPRGWPYADPAEMSLRLLAAARAAGIGITLLP
                     VLYQHAGFGGQPPRADQRRFVNGTDALLEIARRARAHGASVGVAPHSLRAVAPAALHE
                     LVAGIDAAAPIHLHIAEQQPEVDACLAWSGQRPVAWLLDHLPVDARWCLVHATHVDAA
                     ERRALAASGAVAGLCPTTEANLGDGVFPAAGYAADGGAWGIGSDSHASVDVAEELRLL
                     EYTQRLAQQRRNVLVGSASASAQVADHLWLSAVAGGSRAGGGGSAGAQHGLAVGAPAD
                     LVVLDTRGTLAGLTPPQALASHVFANHRPGCVRDVWARGVRRIHDGRHALQADATARF
                     VAARRALLSEV"
     gene            2093..3319
                     /gene="hutI"
                     /locus_tag="VFA35_07815"
     CDS             2093..3319
                     /gene="hutI"
                     /locus_tag="VFA35_07815"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZT56115.1"
                     /translation="MPAAPPIDLLIRNARVVACTDATPAVSPPACIAIADGVITAVGP
                     LDTLASGRDVRAELDAGGALLTPGLIDCHTHLVYAGNRAREFEMRLAGASYEEIARAG
                     GGIVSTVRQTRAASPADLLAASARRLRELMRHGVTTVEIKSGYGLALADELKMLEVAR
                     ALGAQLPIDVRTTLLAAHALPPEFAGRADAYVDLVVEQIVPEAARRGLADAVDAFCEN
                     IAFTPAQTRRVFEAARAHGLPVKLHAEQLSNQHGAALAAEFGALSADHLEHVDEDGVR
                     AMARAGTVAVMLPGAYYFLRDTQAPPIALFRRHGVPMAVATDCNPGTSPGAHLPLMMN
                     MACTLFRMTPAEALLGVTRHAAQALGLSNTRGTVEVGKRADLCLWDVDQPAELAHAIG
                     SSPLRARLQRGVQHPL"
BASE COUNT          399 a         1294 c         1210 g          444 t
ORIGIN      
        1 gctcgtcaac gacagtggct ccggcgtgat gcgccatgcc gatgccggct acgagctcgc
       61 ggtcaagacc gcgcgcgaac agcatctgaa cctgccgatg ctcgcgccgt gagcgcgccg
      121 cccgagcgct tcgacctgcg cgagatcgcc ccgcagccgt ggcagaacgg cgccggcctg
      181 acgcgcgaga tcgcgctcgg tggcgccgac ccggcgcgct tcgactggcg cctgagcgtc
      241 gccgaggtca cgcgcgacgc gccgttctcg gccttcccgg gcgtggaccg gtgcatcgtg
      301 ttgctagccg gcgccggcaa gcgtctgcgc tcgcccgacg gcgcgatcga ccacgcgctg
      361 acccggccgc tcgagccgtt ccggttcccg ggcgaggtgc cgctggccgc gacgctggcc
      421 ggcggcgcga gccgcgactt caacgtgatg atgcgccgcg gcgtgttccg cagcgagctg
      481 cgctgtacgc acgccgccgc cgatctggcg ggcggcgacg tgagcttcgc gctgtgcatc
      541 gccggcgcgt gggcgctcgc cgaggatgcg accagcgcga ccaccgcgcc gatcgagccg
      601 ctgcaggccc tgctctggcg cgcgccggtg gcgcccctgc gcctcgagcc gcgcggcgcc
      661 gcggcgacgc tgctgctggt gcggctgtat cacgatcgcc cgcgatgaac accgcccgcc
      721 tgttcgccga gcacgcgctg ctgcccaccg gctgggcgcg cgatgtgctg ctggtgtggg
      781 acgacgcggg cgtgctgacc gaggtcaccg caggcgcgcc cgcgccgccc ggtgcgcagc
      841 gcgcgctcgg cccgctgctg ccgggcatgc cgaacctgca ttcgcatgcg ttccagcgcg
      901 cgttcgccgg cctgagcgag tggcgcagca gcgccgaggc cgacagcttc tggacctggc
      961 gcgacctgat gtaccgcatc gcgaacgccg tctcgcccga ccagctcgag gcgatcgcga
     1021 cccggctcta tgtcgagatg ctggccgccg gctacaccac cgtctgcgag ttccactacc
     1081 tgcaccacga cccgcgcggt tggccgtatg ccgatccggc cgagatgtcg ctgcgcctgc
     1141 tggccgcggc ccgcgccgcc ggcatcggca tcacgctgct gccggtgctg taccagcacg
     1201 ccggcttcgg cggccagccg ccgcgcgccg accagcggcg cttcgtcaac ggcaccgacg
     1261 cgctgctcga gatcgcgcgg cgcgcgcgcg cccacggcgc gagcgtcggc gtcgcgccgc
     1321 actcgctgcg cgcggtcgcg ccggcggcgc tgcacgagct ggtcgcgggc atcgacgcgg
     1381 cggcgccgat tcatctccac atcgccgagc agcagccgga ggtcgacgcc tgcctggcct
     1441 ggagcggcca gcggccggtg gcgtggctgc tcgatcacct gccggtcgac gcccgctggt
     1501 gcctggtcca cgcgacccac gtcgacgccg ccgaacgccg cgcgctcgcg gccagcggcg
     1561 cggtcgccgg gctgtgcccg acgaccgaag cgaacctcgg cgacggcgtg tttcccgctg
     1621 ccggctacgc cgccgacggc ggcgcctggg gcatcggctc cgacagccac gccagcgtcg
     1681 acgtggccga ggaactgcgc ctgctcgagt acacgcagcg cctcgcgcag cagcgccgca
     1741 acgtgctcgt cggcagcgcg agcgcgagcg cgcaggtcgc cgatcacctg tggctcagcg
     1801 cggtggcggg cggctcgcgg gccggcggcg gcggctccgc cggcgcgcag cacgggctcg
     1861 cggtcggcgc gccggccgac ctggtcgtgc tcgacacccg cggcacgctg gccggcctca
     1921 cgccgccgca ggcgctggcc agccacgtgt tcgcgaacca ccggcctggc tgcgtgcgcg
     1981 acgtctgggc gcgcggcgtg cgccgcattc acgacggccg ccacgccttg caggccgacg
     2041 cgaccgcccg tttcgtcgcg gcccgccgcg ccctgctcag cgaggtctga ccatgcccgc
     2101 cgccccgccg atcgacctgc tgatccgcaa cgcgcgcgtc gtcgcctgca ccgatgccac
     2161 cccggccgtt tcgccgccgg cctgcatcgc gattgcggac ggcgtgatca cggccgtcgg
     2221 cccgctcgac acgttggcat cgggccgcga cgtgcgcgcc gaactcgatg ccggcggcgc
     2281 gctgctgacg cccggcctga tcgattgcca cacccacctc gtctacgccg gcaaccgcgc
     2341 acgcgagttc gagatgcggc tggcgggtgc gagctacgaa gagatcgcac gcgccggcgg
     2401 cggcatcgtc tcgacggtgc gccagacccg cgccgccagc ccggccgacc tgctcgccgc
     2461 atcggcacgc cggctgcgcg agttgatgcg gcacggcgtc acaacggtcg agatcaagtc
     2521 cggctacggg ctggcgctgg ccgacgagct gaagatgctg gaggtcgcgc gcgcgctcgg
     2581 cgcgcagttg ccgatcgacg tgcgcacgac gctgctggcc gcgcacgcgc tgccgcccga
     2641 gttcgccggc cgcgccgacg cgtatgtcga cctggtcgtc gagcagatcg tgccggaggc
     2701 cgcgcgccgc gggctggccg acgccgtcga cgccttctgc gagaacatcg cgttcacgcc
     2761 ggcgcagacg cggcgcgtgt tcgaggcggc gcgcgcgcac ggcctgccgg tcaagctgca
     2821 tgccgagcag ctctcgaacc agcacggtgc ggcgctcgcg gccgagttcg gcgcgctctc
     2881 ggccgaccat ctcgaacacg tcgacgaaga cggcgtgcgc gcgatggcgc gcgccggcac
     2941 cgtcgcggtg atgctgcccg gcgcgtacta cttcctgcgc gacacgcagg cgccgccgat
     3001 cgcgctgttc cgccgccacg gcgtgccgat ggcggtggca accgactgca accccggcac
     3061 cagccccggc gcgcatctgc cgctgatgat gaacatggcg tgcacgctgt tccgcatgac
     3121 gccggccgag gcgctgctcg gcgtcacgcg ccatgccgcg caggcgctcg gcctgtcgaa
     3181 cacgcgcggc accgtcgagg tcggcaagcg cgccgatctg tgtttgtggg atgtcgacca
     3241 gccggccgag ttggcgcatg cgatcggctc aagcccgctg cgggcgcgcc tgcagcgcgg
     3301 cgttcagcat ccgctgtagc agcggttgta ctttctcggc caggtca
//
LOCUS       DASRDG010000470         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_184043, whole genome shotgun sequence.
ACCESSION   DASRDG010000470 DASRDG010000000
VERSION     DASRDG010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184043"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFA35_07820"
     CDS             complement(<1..565)
                     /locus_tag="VFA35_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56116.1"
                     /translation="MTATALNVIDLKTGLPHTGLTAHEHVGFELGWDYAHYRVTPPAP
                     YTQEPSPLRQGLLAGQAVFGTRTLAATRHVRKWLQLRLHAWLRGRSVELVQVTPNYLQ
                     QIDASHCPITRVALSSATLEASDASVDRVRNDAGYAAGNLAVMSTKANHAKAAFGLRD
                     AQRFVQQIEAEQLPGLGGLTAAQWSRVA"
     gene            728..1744
                     /locus_tag="VFA35_07825"
     CDS             728..1744
                     /locus_tag="VFA35_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZT56117.1"
                     /translation="MDRTERFYKIELLLKSRGCVSFAALQDELSVSPATLKRDLQYLR
                     DRLSAPIVYDAFANGYRFAETKAGAPGATRHELPGLWFSENEIHALLTMHQLLKGLDD
                     DGVLARHLQPLMEKLQGMLGADESAARELMRRVKVIGTARRRVPSRHFELLGSALLQR
                     RRLRLRYFKRSDRSTSEREVSPQRLVNYRNTWYLDAWCHASDGLRRFALDAVRDATLL
                     DAKAKNVALRELEAALDAGYGIYGGGDAKVKWATLVFSAEAAQWVANEEWHPQQKARW
                     LEPDPRGTPGERRYELQVPYSEPTELAMDILRHGDSVVVTGDKALLALIGRRLRSAAA
                     QYAG"
     gene            1787..2065
                     /locus_tag="VFA35_07830"
     CDS             1787..2065
                     /locus_tag="VFA35_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HZT56118.1"
                     /translation="MKATWNGVTIAESDDTVIVEGNHYFPENSLKREYVSFSNHRSSC
                     AWKGQAHYYSLFVGGELNENAVWYYPEPSEAAQAIKGRVAFWKGVKVA"
     gene            2062..2895
                     /locus_tag="VFA35_07835"
     CDS             2062..2895
                     /locus_tag="VFA35_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT56119.1"
                     /translation="MNGPLARWLFRLTCTALGTAALGCAAPVQPQPQTTLEVFAAGSL
                     RAALTDIAKAFEAAEPGTTVRLTFGASGLLKERLAGGERADVFASANMEHPQALFANG
                     RGGPVRRFAQNRLCVLAPQALPLTPDTLIDRMLDPALKLGTSTPKADPSGDYAWLMFE
                     RVEQAGRAGAFKALSAKALQLTGGPQSPPPPAGQSVYGALVAQGHADLFVTYCTNAAN
                     ALREQPQLKIVTVPAAINVSASYGLSTLAGAAPAANRFVEFVLGPTGQGILARLGFAP
                     A"
BASE COUNT          465 a         1014 c         1029 g          393 t
ORIGIN      
        1 tggccacgcg cgaccactgc gccgcggtca gcccgcccag gccgggcagt tgctcggcct
       61 cgatctgctg cacgaagcgc tgcgcgtcgc gcaagccgaa cgccgccttc gcgtggttcg
      121 ccttcgtgct catcaccgcg aggttgccgg cggcgtagcc ggcgtcgttg cgcacgcggt
      181 cgaccgacgc atcgctcgcc tccagcgtgg cgctcgacag cgcgacgcgg gtgatcgggc
      241 agtggctcgc atcgatctgc tgcaggtagt tcggcgtgac ctgcaccagc tcgacgctgc
      301 gcccgcgcag ccacgcgtgc agccgcagct gcagccactt gcgcacgtgg cgcgtcgcgg
      361 ccagcgtgcg cgtgccgaac acggcctggc cggcgagcag gccctgacgc agcggcgagg
      421 gctcctgcgt gtacggggcg ggcggcgtga cgcggtagtg ggcgtagtcc cagccgagct
      481 cgaagccgac gtgctcgtgc gccgtcaggc cggtgtgggg gaggccggtt ttcagatcga
      541 tcacgttcag cgcggtggca gtcatggcgg agctccgagg cattcgccgc gaaccgttcg
      601 cagcgatggc gccagtgttg cggccaggta gctcagagcg tgagccacca agaacgagcc
      661 gactcggggt atgctctgcg ccactggctc accccatgag ccaccgagct gatggagaat
      721 caccacgatg gaccgcaccg agcgcttcta caagatcgaa ctgctgctga agagccgcgg
      781 ctgcgtcagc ttcgccgcgc tgcaggacga actctcggtc tcgcccgcca ccctgaagcg
      841 cgacctgcag tacctgcgcg accgcctgag cgcgccgatc gtctacgacg cgttcgcgaa
      901 cggctaccgg ttcgccgaga ccaaggccgg cgcgccgggc gcgacacgcc acgagctgcc
      961 cggcctgtgg ttcagcgaga acgagatcca cgcgctgctg acgatgcacc agctgctcaa
     1021 gggcctggac gacgacggcg tgctggcgcg ccacctgcag ccgctgatgg agaagctgca
     1081 gggcatgctc ggcgccgacg agagcgccgc gcgcgagctg atgcgccgcg tgaaggtcat
     1141 cggcacggcg cgccggcgcg tgccgagccg gcatttcgag ctgctcggca gcgcgctgct
     1201 gcagcggcgt cgcctgcggc tgcgctactt caagcgcagc gaccgcagca ccagcgagcg
     1261 cgaggtctcg ccgcaacgac tcgtcaacta ccgcaacacc tggtacctcg acgcctggtg
     1321 ccacgcgagc gacggcctgc gccgcttcgc gctcgatgcg gtgcgcgacg cgacgctgct
     1381 cgacgcgaag gcgaagaacg tcgcgctgcg cgaactggag gccgcgctcg acgccggcta
     1441 cggcatctat ggcggcggcg acgcaaaggt caagtgggcg acgctggtgt tcagcgccga
     1501 ggccgcgcag tgggtcgcga acgaggagtg gcacccgcag caaaaggcgc gctggctgga
     1561 gccggaccca cgcggcacgc cgggcgagcg ccgctacgaa ttgcaggtgc cgtacagcga
     1621 gccgaccgaa ctcgcgatgg acatcctgcg ccacggcgac agcgtcgtcg tgaccggcga
     1681 caaggcgctg ctggccctga tcggccggcg gctgcgcagc gcggcggcgc aatatgccgg
     1741 atgatggcgc cccggaccca gacccagaaa caccgatgga gacccgatga aggccacctg
     1801 gaacggcgtg acgatcgccg aaagcgacga caccgtgatc gtcgaaggca accactactt
     1861 tcccgagaac agcctgaagc gcgaatacgt gagcttcagc aaccaccgca gcagttgcgc
     1921 gtggaagggg caggcgcact attacagcct gttcgtgggc ggcgagctga acgagaacgc
     1981 ggtctggtat tacccggagc cgagcgaggc ggcgcaggcg atcaagggcc gggtcgcgtt
     2041 ctggaagggc gtgaaggtcg catgaacggg ccgctcgcac gctggttgtt tcgtttgacc
     2101 tgcaccgcac tcggcaccgc tgcgctgggc tgcgcggcgc cggtgcagcc gcagccgcag
     2161 acgacgctcg aggtcttcgc cgccggcagc ctgcgcgccg cgctgaccga catcgccaag
     2221 gccttcgagg ccgccgagcc cggcaccacg gtgcggctga ccttcggcgc gtcgggcctg
     2281 ttgaaggagc ggctcgccgg cggagagcgt gccgacgtgt tcgcctcggc caacatggag
     2341 cacccgcagg ccctgttcgc gaacggccgc ggcggcccgg tgcggcgctt cgcgcagaac
     2401 cggctgtgcg tgctggcgcc gcaagcgctg ccgctgaccc ccgacaccct gatcgaccgc
     2461 atgctcgacc cggcgttgaa gctcggcacc tcgacgccga aggccgaccc ctcgggcgac
     2521 tacgcctggc tgatgttcga gcgcgtcgag caagccggcc gtgccggtgc cttcaaggcg
     2581 ctcagcgcca aggcgctgca gctgacaggc gggccgcaat cgccgccgcc gccggccggg
     2641 cagagcgtct acggcgcgct ggtcgcgcag ggccacgccg acttgttcgt cacctattgc
     2701 accaatgccg cgaacgcgct gcgcgaacaa ccgcaactga agatcgtcac ggtgccggcg
     2761 gcgatcaacg tcagcgccag ctacggcctg agcacactcg ccggcgcggc gccggccgcg
     2821 aaccgcttcg tcgagttcgt gctcgggccg accgggcagg gcatcctggc gcgtttggga
     2881 tttgcgccgg catgagccct a
//
LOCUS       DASRDG010000471         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1222570, whole genome shotgun sequence.
ACCESSION   DASRDG010000471 DASRDG010000000
VERSION     DASRDG010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222570"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFA35_07840"
     CDS             <1..217
                     /locus_tag="VFA35_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZT56120.1"
                     /translation="SALAASESAYDLATQRYKAGLGSYLTVLNAEAAVLNQRRLGADL
                     KARALEVQIGLIRALGGGYVADQQARG"
     gene            254..1618
                     /locus_tag="VFA35_07845"
     CDS             254..1618
                     /locus_tag="VFA35_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZT56121.1"
                     /translation="MTAPETTTPAAVPQGNPARKRALTAVTAAVAVAGIAYGAYWALV
                     LNHYESTDNAYVQGNVVQLTPQVGGTVVAINADDTDHVKAGQLLVKLDPADAQVALDQ
                     AEAQLAQTVREVRTLYANNSTLKAQIALREADLARANSDAARAQDDVARRQPLVATGA
                     VGKEEFSHSTASLAAAKSSVVAAQSAVVAAREQLVSNQTLTEGTAVDQHPNVLRASAR
                     VRESYLALHRADLLAPVDGFVAKRSVQLGQRVPAGMPLMSVIALNQVWVDANFKESQL
                     KNLRIGQPVTLEADVYGKKVEYAGRIEGLGAGTGAAFALLPAQNATGNWIKVVQRVPV
                     RIALDPKALAAHPLRVGLSMEAKVDISKTDGAMLADGSRAATSAQTGVFDDNHGAADA
                     EVRKIIAANGGGVGGKLDAKVGAPDVAVAKTRKPTPVAAAVGEAAVATIAGSHAAPPA
                     ATLK"
     gene            1638..>2758
                     /locus_tag="VFA35_07850"
     CDS             1638..>2758
                     /locus_tag="VFA35_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZT56122.1"
                     /translation="MSSTTAALPAGAGDLPATPPAPPAKPVAFPPLTGSQLVLGTVAL
                     SLATFMNVLDTSIANVSIPAISGDLGVSPSQGTWVITSFAVANAISVPLTGWLTQRFG
                     QVRLFTMSVLLFVIASWLCGLAPNMGMLIAFRVLQGFVAGPMIPLSQTLLLASYPAAK
                     AGTAMAMWAMTVLVAPVAGPLLGGWITDNIAWPWIFYINIPVGIVAAGLTWSIYHQRD
                     PGPRRVPLDVVGLGLLVLFVGAMQIMIDKGKELDWFNSGQIVMLAIVASVGFLFFLAW
                     ELTETHPIVDLRLFAQRNFLMATAALSVAYGVFFGNVVLLPLWLQQYMGYTATWAGLA
                     TAPVGILAIILSPWVGKNVSKIDPRKLATVAFVGFGIVL"
BASE COUNT          405 a          996 c          965 g          392 t
ORIGIN      
        1 gagcgcgctg gccgcgtccg aatcggccta cgacctcgcg acgcagcgct acaaggccgg
       61 cctgggcagc tacctgacgg tgctgaacgc cgaagccgcc gtgctgaacc agcgccgcct
      121 cggcgccgac ctgaaggcgc gcgcgctgga ggtgcagatc ggcctgatcc gcgccctcgg
      181 cggcgggtac gttgccgatc agcaggcgcg cggctgacgt cacgaacagc gaaacgacct
      241 tccggagatc ctcatgaccg cccccgaaac caccaccccc gccgccgttc cgcaaggcaa
      301 cccggcccgc aagcgcgcgc tgacggcggt gaccgccgcc gtcgcggtcg ccggcatcgc
      361 ctacggcgcc tactgggcgc tggtgctgaa ccactacgag tcgaccgaca acgcctacgt
      421 gcagggcaac gtcgtgcagc tgacgccgca ggtcggcggc acggtcgtcg cgatcaacgc
      481 cgacgacacc gaccacgtca aggccggcca gctgctcgtc aagctcgacc cggcggatgc
      541 gcaggtcgca ctcgaccagg ccgaggcgca gctcgcgcag acggtgcgcg aggtgcgcac
      601 gctgtacgcg aacaacagca cgctgaaggc gcaaatcgca ctgcgcgagg ccgacctggc
      661 acgcgcgaac agcgacgcgg cgcgcgcgca ggacgacgtg gcgcggcgcc agccgttggt
      721 cgcgaccggc gcggtcggca aggaggagtt cagccactcg accgcgtcgc tggccgccgc
      781 gaagagcagc gtcgtcgccg cgcagtcggc agtcgtcgcc gcgcgcgagc agctcgtgtc
      841 gaaccagacg ctgaccgaag gcaccgcggt cgaccagcac ccgaacgtgc tgcgcgcctc
      901 ggcgcgcgtg cgcgagtcct acctcgcgtt gcatcgcgcc gatctgctgg cgccggtcga
      961 cggcttcgtc gccaagcgca gcgtgcagct cggccagcgc gtgccggccg gcatgccgct
     1021 gatgtcggtg atcgcgctga accaggtctg ggtcgacgcg aacttcaagg aaagccagct
     1081 gaagaacctg cgcatcggcc agccggtgac gctcgaagcc gacgtgtacg gcaagaaggt
     1141 cgagtacgcc ggccggatcg agggcctggg cgcgggcacc ggcgcggcgt tcgcgctgct
     1201 gccggcgcag aacgcgaccg gcaactggat caaggtggtg cagcgcgtgc cggtgcgcat
     1261 cgcgctcgac ccgaaggcgc tggccgcgca cccgctgcgc gtcggcctgt cgatggaggc
     1321 gaaggtcgac atcagcaaga ccgatggcgc gatgctggcc gacggctcgc gcgccgcgac
     1381 gagcgcgcag accggcgtgt tcgacgacaa ccacggcgcg gccgacgccg aggtgcgcaa
     1441 gatcatcgcc gccaatggtg gcggggtcgg cggcaagctc gacgccaagg tcggcgcgcc
     1501 cgatgtcgcc gtcgcgaaga cgcgcaagcc cacgccggtc gccgccgcgg tcggcgaggc
     1561 cgcggtcgcg acgatcgccg gctcgcacgc cgcgccgccc gccgccacgc tgaagtgacc
     1621 cgccggagcg cctgagcatg tcctccacca ccgccgcgct gcccgccggc gccggcgatc
     1681 tgccggccac gccgcccgca ccgcccgcga agcccgtcgc gttcccgccg ctgaccggca
     1741 gccagctcgt gctgggcacg gtcgcgctgt cgctcgcgac cttcatgaac gtgctcgaca
     1801 cctcgatcgc gaacgtctcg atcccggcga tctcgggcga cctgggcgtg agcccgtccc
     1861 aaggcacctg ggtcatcacc tcgttcgcgg tcgcgaacgc gatctcggtg ccgctgaccg
     1921 gctggctgac gcagcgcttc ggccaggtgc gcctgttcac gatgagcgtg ctgctgttcg
     1981 tgatcgcgtc gtggctgtgc gggctggcgc ccaacatggg catgctgatc gccttccgcg
     2041 tgctgcaggg cttcgtcgcc gggccgatga tcccgctctc gcagacgctg ctgctcgcga
     2101 gctacccggc cgcgaaggcc ggcaccgcga tggcgatgtg ggcgatgacg gtgttggtgg
     2161 cgccggtcgc cggcccgctg ctcggcggct ggatcaccga caacatcgcc tggccgtgga
     2221 tcttctacat caacatcccg gtcggcatcg tcgcggccgg cctgacctgg tcgatctacc
     2281 accagcgtga cccggggccc cggcgcgtgc cgctcgacgt cgtgggcctg ggcctgctgg
     2341 tgctgttcgt cggcgcgatg cagatcatga tcgacaaggg caaggagctc gactggttca
     2401 actcgggcca gatcgtcatg ctggcgatcg ttgcgtcggt gggcttcctg ttcttcctgg
     2461 cctgggagct gaccgagacg cacccgatcg tcgacctgcg cctcttcgcc cagcgcaact
     2521 tcctgatggc gaccgcggcg ctgagcgtgg cctacggcgt gttcttcggc aacgtcgtgc
     2581 tgctgccgct gtggttgcag cagtacatgg gctacaccgc gacgtgggcc ggcctggcga
     2641 ccgcgccggt gggcatcctc gccatcattc tttcgccgtg ggtcggcaag aacgtcagca
     2701 agatcgatcc gcgcaagctc gcgacggtgg ccttcgtcgg cttcggcatc gtcctgtg
//
LOCUS       DASRDG010000472         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_996539, whole genome shotgun sequence.
ACCESSION   DASRDG010000472 DASRDG010000000
VERSION     DASRDG010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996539"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFA35_07855"
     CDS             <1..977
                     /locus_tag="VFA35_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZT56123.1"
                     /translation="ERRVPQDGRLQLAYASRTIDVRVSIMPSIHGEDAVLRVLDRQHL
                     AESLAGLTLAKLGFDEPSAEALRQLCRKPHGMLLVTGPTGSGKTTTLYAAIAETHQSR
                     DKIITIEDPVEYQLPGVLQIPVNERKGLTFARGLRSILRHDPDKILVGEIRDAETAQI
                     AVQSALTGHLVFTTVHANTVFDVVGRFEQFGIDAYTFASAINGVLAQRLLRRVCGHCA
                     VPVDPTIQQAVDQDLRAAMQRLVGVPRLMQARGCEHCRGTGYAGRFALGELLHLAPAL
                     KALVSARAPQAQIQAEAERTGWRSLRGLAIDAALRGHTTLEEVDRVAA"
     gene            964..1824
                     /locus_tag="VFA35_07860"
     CDS             964..1824
                     /locus_tag="VFA35_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56124.1"
                     /translation="MWPLERKRDIVRVGRERAERWAWNGERLILRGQETMDCAAGPDA
                     AVLAAALRMLFHSARERGTRGEPSREVDVVFESAWLPVMSLELGRKLWSRTQVEALLR
                     HRLGQLHARRDDPVGDWELQLDHRAGDAHALGYGLAPSVKQAAMNAAAATGRQLASLQ
                     PVFAWGWQRLSRHHRNLGGARSGWWVWIEQDRSLVCRIDRGRVIAVNPGAAVPRDDPQ
                     CLRLIQIEASRLGVLGPVGRGVAATWDGAESAVRGANDEARMSWVSVAAREIDPPRDR
                     PAQPNAEAAA"
     gene            1821..2345
                     /locus_tag="VFA35_07865"
     CDS             1821..2345
                     /locus_tag="VFA35_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56125.1"
                     /translation="MSVGAIDFLHRRRSPWLGWCLLAIGAAALSASLWLDQRWTAERA
                     HREAVARTRDEAMQQARRAAMRPLPPTADQRRLQRIASQLRQPWLPVLRVIENITEAP
                     VFLLALSIDPATGIVQLDGEAPNFDYAMAYAQLLDEEGLLGPAELRSHELVTEPGTGR
                     ATLRFTIVTRWSAR"
     gene            2342..2923
                     /locus_tag="VFA35_07870"
     CDS             2342..2923
                     /locus_tag="VFA35_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56126.1"
                     /translation="MSGAVARGPALALTILIARLRQRVGVPGLLGLGLVAVAAAAMSL
                     AWRQHQRLDLAPSAPGLPMVHAVPATMPRPPKTEMPEHAALPPVSDIPILLTRIQRAA
                     LDAGLGWPRADYRVNAATEDAPASLEVRCTLKGPYPSIRRFVTTLLQDAPTLTLREFG
                     LSRANADAGGVEAKLSFTVYLASGASHAIGLPR"
BASE COUNT          455 a         1029 c         1127 g          502 t
ORIGIN      
        1 ctgaacgtcg cgtgccccag gacggacgcc tgcagctcgc ctatgccagt cgcacgatcg
       61 acgtgcgcgt atcgatcatg cccagcatcc acggcgagga tgcggtcttg cgggtcctgg
      121 accgccagca cctggccgaa agcctggcgg gcctgacgct ggcgaaactc ggtttcgacg
      181 aaccctctgc cgaggcactg cgccagctgt gccgcaagcc gcacggcatg ctgctggtga
      241 ccggtccgac cggttcgggc aagaccacca ccttgtacgc ggcgatcgcc gagacgcatc
      301 agtcgcgcga caagatcatc acgatcgaag acccggtcga atatcagttg cccggcgtgc
      361 tgcagatccc ggtgaacgag cgcaaggggc tgaccttcgc ccgcggcctg cgctccatct
      421 tgcgccacga cccggacaag atcctggttg gcgagatccg cgacgccgag acggcgcaga
      481 tcgcggtgca gtcggcgctg acgggccacc tggtgttcac gacggtgcac gccaacacgg
      541 tgttcgacgt ggtcgggcgt ttcgagcaat tcggcatcga tgcctacacc ttcgcgtcgg
      601 ctatcaacgg cgtgctggcg cagcggctgt tgcgccgggt ctgcggccat tgcgcggtcc
      661 cggtcgatcc gaccatccag caggcggtgg accaggacct gcgcgcggcg atgcagcgct
      721 tggtcggtgt gccgcgcctg atgcaggccc gcggctgcga gcactgccgc ggcaccggct
      781 atgccggacg cttcgcgctc ggcgaactgc tgcacttggc gcccgcgttg aaggcgctgg
      841 tgtccgcgcg agcgccgcag gcgcaaatcc aggcggaggc cgagcggacc ggctggcgca
      901 gcctgcgcgg gctggcgatc gacgccgcac tgcgggggca taccacgctc gaggaggtgg
      961 accgtgtggc cgcttgagcg caagcgcgac atcgtgcgcg tcggccgcga gcgggccgag
     1021 cgctgggctt ggaacggcga gcgattgatc ttgcgcggcc aggagacgat ggactgcgcg
     1081 gccggtccgg acgccgccgt gcttgcggcg gccctgcgca tgttgttcca tagcgcacgc
     1141 gaacggggaa cgcgcggcga gccatcgcgc gaagtcgacg tggtgttcga gtctgcctgg
     1201 ctgccagtga tgtcgctcga actcggtcgc aaactttggt cgcgcaccca ggtcgaagca
     1261 ttgctgcgcc atcggctcgg acagcttcat gcacggcgcg atgatccggt gggtgactgg
     1321 gagttgcaac tcgaccaccg tgccggcgat gcgcatgcac tgggctatgg gctggcgcca
     1381 tcggtgaagc aggcggcgat gaacgccgcg gcggcgactg ggcgccaact cgcgtctctg
     1441 cagccagtct tcgcctgggg ttggcagcga ttgagtcgcc accaccgaaa tctcggcggt
     1501 gcgcgcagcg gctggtgggt gtggatcgaa caggatcggt ctctggtgtg ccgcattgac
     1561 cgcggtcgcg tgatcgctgt gaaccccggc gcggcagtgc cgcgcgacga cccgcaatgc
     1621 ctgcgtttga tccagatcga ggcatcgcgc ctgggtgtgc tggggccggt gggccggggc
     1681 gtggcggcga cctgggatgg cgccgaatcc gcggtccgtg gggccaatga tgaggctcga
     1741 atgtcctggg tctcggtcgc cgcgcgggag atcgacccac cgcgggacag gccggcgcaa
     1801 ccgaacgccg aagcggccgc atgagcgtcg gcgccatcga tttcctgcac cggcggcggt
     1861 cgccgtggct cggatggtgc ctgctcgcca tcggcgcagc ggcgctgtcg gcgagcttgt
     1921 ggctcgacca gcgctggacc gcagaacgtg cccaccggga ggccgtcgcg cgcactcgcg
     1981 acgaagcgat gcaacaggcg cgccgcgctg cgatgcgacc ccttcctccg accgccgacc
     2041 agcgtcgact ccaacgcatt gcgtcacagc tgcgacagcc ctggcttcca gtactgcggg
     2101 tcatcgagaa catcaccgag gcgccagtct tcctgctggc actgtcgatc gatccggcga
     2161 cggggatcgt gcagctcgac ggagaggcgc cgaacttcga ctacgccatg gcgtacgcgc
     2221 aactgctgga cgaagaaggg ttgctggggc cggcggagtt gcgttcgcac gaactcgtca
     2281 ccgagcctgg cacgggtcgc gcgaccctgc gcttcacgat cgtgacccgc tggagcgcgc
     2341 gatgagcgga gccgtcgccc gcggtccggc cctggcactg acgatcctca ttgcgcgctt
     2401 gcgccagcgc gtcggtgtac ccggcctgtt gggcctgggt cttgtggccg tggcggccgc
     2461 agcgatgtcg ctcgcctggc ggcagcatca gcggctcgat ctcgcgccgt ccgcgcccgg
     2521 attgccgatg gtgcacgcag tgccggcaac catgccccgg ccgccgaaga cggaaatgcc
     2581 ggaacatgcc gcgttaccgc cggtctcgga cattccgata ctgctgaccc gcatccagcg
     2641 tgccgcgctg gatgcgggtc ttggctggcc gcgggcggac tatcgggtca atgccgcgac
     2701 agaggacgcg ccggccagcc tggaggtgcg ctgtacgctg aaggggccgt acccgagcat
     2761 tcgccggttc gtgacgacgc tgctgcagga cgcgccgacg ctgacgctgc gcgagttcgg
     2821 cttgagccgg gccaatgcgg atgccggcgg ggtggaggcc aagctctcct tcaccgtcta
     2881 cctcgcctcc ggcgcgtcgc atgccatcgg gttgccgcga tgaagcagcg tcgcttggcc
     2941 gtgctgcttg ccgtactggt cgtgttgctg gggctgcgct ggtgggatcc gccgatgggc
     3001 accgcgtcga ccgaggtgtc cgaagcggtg gtgaagccgg cggcgcatgc atcgtccgag
     3061 cttgctggca atgtgccggt agacagcgtc ggggcagtgg ctggtccgcc cga
//
LOCUS       DASRDG010000473         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1676863, whole genome shotgun sequence.
ACCESSION   DASRDG010000473 DASRDG010000000
VERSION     DASRDG010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676863"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1544
                     /locus_tag="VFA35_07875"
     CDS             141..1544
                     /locus_tag="VFA35_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZT56127.1"
                     /translation="MDAREFAPAAATRDMYLDSQGNPVPGLSTDSALAAGIPGEPAGM
                     ALLAARYGRLPVARSLAPAIRLARDGFPLSKHLQDGLNARLAQFKTEPEVARNFLRDG
                     AVPELGTVIRQPELAGTLEYLARHGLDNFYSGEFAHKLADGVRAQGGTWTVADLAAYH
                     ALERAVVIGNYRGARIVSAPPPSSGGVGLVEALNVLAQFDLKRATPLQRKQLVIEAMR
                     RAHRDRAVYLGDADFVSVPVARLTSLDYAAGLAAGIRGDHATPSSSLPGVGPGANVGP
                     STTHFSVLDRDGNRVAATITLNLRFGSGRMIAGTGLFLNNEMDDFSVKPNSPNAFALI
                     GGDANAIAPHKRMLSSVTPCFVEAGGRVMIGGSPGGSLIMGMSLLATLDFIDGHSASE
                     IVAAPRYHHQYLPDIVRYEAGAFSDDEIAALGSEGYTLQATSFRYGNFQVVTWDRATG
                     AVEAAADPRGEGAGVVE"
     gene            1564..1947
                     /locus_tag="VFA35_07880"
     CDS             1564..1947
                     /locus_tag="VFA35_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HZT56128.1"
                     /translation="MQLRQECELALRGDWVPVAAEVSGQVLPLADLRVSVLSFEDDRY
                     LIIDHAGQVADAGRWQLGAEASPATLDLEGNEGPNAGRRVLAIVAIDGDRLCLGYDME
                     REQRPGSWRHEPDQLLLCITYLRRR"
     gene            complement(1951..>2141)
                     /locus_tag="VFA35_07885"
     CDS             complement(1951..>2141)
                     /locus_tag="VFA35_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HZT56129.1"
                     /translation="EYTDMQVTMHGDAAIVTGIFSGKGTDTAGKPVNVHERFTDTWVK
                     TADGKWLCVASHGSNIAT"
BASE COUNT          330 a          734 c          740 g          337 t
ORIGIN      
        1 gaactggcga tggcggctcg tcgcggcgcg ctcgcgagcg cagcgggccg caccgccggc
       61 cggacaacag gtgcaaccgt ggccggcggg gcggctactg cagcgatcga ccaccacagc
      121 gcgacggact cgaggttgcg atcgacgcgc gcgaatttgc gcctgcggcc gcgacgcgcg
      181 acatgtacct cgattcgcag ggcaaccccg tgcccggact gtcgaccgac agtgcactgg
      241 ccgcgggcat accgggcgag ccggctggca tggcgctgct cgcggcccgc tacggacgcc
      301 tgccggttgc gcgcagcctg gcaccggcca tccgcctggc gcgcgacggg ttccccttgt
      361 cgaagcactt gcaggacgga ttgaacgcac gactcgcgca attcaagacc gaacccgagg
      421 tcgcgcgcaa cttcctgcgc gacggcgcgg tgccggaact cgggaccgtg atacggcaac
      481 cagagctggc cggcacgctc gagtacctcg cacgccacgg gctcgacaac ttttacagcg
      541 gggagttcgc ccacaaactc gcggacgggg tgcgcgcgca aggcggcacg tggaccgtgg
      601 ccgacctcgc ggcctaccat gcgctcgagc gcgccgtagt catcggcaac tatcgtggcg
      661 cgcgcatcgt gtcggcgccg ccgccaagct cgggcggcgt cggcctcgtc gaggcactca
      721 acgttctcgc gcaattcgac ctgaagcgag ccacgccgct ccagcgcaag cagctcgtca
      781 tcgaggccat gcgccgcgca catcgcgacc gggccgtgta cctcggcgat gccgacttcg
      841 tcagcgttcc ggtggcgcgc ctgacgagcc tcgactacgc cgccggcctc gcggccggca
      901 tacgtggcga ccacgccacg ccgagcagct ccttgcccgg tgtcgggccg ggcgcgaacg
      961 ttgggccttc gacgacgcac ttttcggtgc tggatcgcga cggcaatcgc gtcgccgcca
     1021 cgatcacgct gaacctgcgt ttcggaagcg gccggatgat cgccggcacc gggctgttcc
     1081 tcaacaatga aatggatgat ttctcggtga agcccaactc gccgaacgct tttgccctga
     1141 tcggcggcga cgccaatgcg attgcgcctc acaagcgcat gctctcgtcg gtgacgccgt
     1201 gcttcgtcga ggccggcggc cgcgtcatga ttggcggctc gccgggtggg agcctgatca
     1261 tgggcatgtc gttgctggcg acgctggatt tcatcgacgg gcactcagcg agcgagatcg
     1321 tggccgcgcc gcgctaccac caccagtacc tgcccgacat cgtgcgctac gaggccggcg
     1381 cgttcagcga tgatgagatt gccgcgcttg gctccgaagg ctacacgctg caggccacct
     1441 cgttccgcta cgggaacttc caggtcgtca cctgggatcg cgcgaccggc gccgtcgaag
     1501 ccgcggccga tccgcgcggc gaaggtgccg gagtggtcga gtaggtgact gcccggggcg
     1561 ccaatgcagt tgcgccaaga gtgtgaactg gccctgaggg gcgactgggt gccggtggcg
     1621 gcggaagtat cggggcaggt tctgccgctc gctgacttgc gcgtatcggt gttgagcttc
     1681 gaggacgatc gctacttgat catcgaccac gccgggcagg tcgccgacgc cggacgatgg
     1741 cagctcggag ccgaggccag tcccgctacg ctcgacctcg aggggaacga aggcccgaac
     1801 gccgggcggc gcgtgctggc gatcgtcgcg atcgacggcg atcgcctgtg cctcggctac
     1861 gacatggagc gcgagcagcg gccgggcagc tggcggcacg agccggacca gctgctgctg
     1921 tgcattacct acctgcgccg gcggtaatcg ctacgtcgcg atgttcgagc cgtgcgaagc
     1981 gacgcacagc cacttgccgt ccgccgtctt cacccaggtg tcggtgaagc gctcgtgcac
     2041 attgacgggc ttgcccgccg tgtccgtgcc cttgccgctg aagatgccgg tcacgatggc
     2101 cgcgtcgccg tgcatcgtca cctgcatgtc ggtgtactcg g
//
LOCUS       DASRDG010000474         4735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_779776, whole genome shotgun sequence.
ACCESSION   DASRDG010000474 DASRDG010000000
VERSION     DASRDG010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779776"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /gene="menA"
                     /locus_tag="VFA35_07890"
     CDS             complement(<1..699)
                     /gene="menA"
                     /locus_tag="VFA35_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HZT56130.1"
                     /translation="MVGHDSSVRMRFATSLRAWAMASRPHTLTIGINPVLVGCALAWA
                     ETGRIDIPLMLLSALGALLLQTGTNLDNDVSDSERGTDRAGRLGLPRATALGLLGPGQ
                     VRSASRACFALATAIGLVLAWRGGWPILIAGIAAAAAAMGYSGGARPISYTPFGDVVV
                     WLFFGLVAVGGTYYLQTGSLSSGALTAATMVGFPATAVLVVNNYRDLDPDAQVGKRTL
                     AVCLGRTFSRWEYAV"
     gene            999..1289
                     /locus_tag="VFA35_07895"
     CDS             999..1289
                     /locus_tag="VFA35_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56131.1"
                     /translation="MLVAEREAREAIEQARLEVDRIVERARLGARALDERTERRIRAV
                     VDAFERELAERLAEIEAAAEQAARPQPFSDDELAALQRAVRALATELIMAPP"
     gene            1286..2053
                     /locus_tag="VFA35_07900"
     CDS             1286..2053
                     /locus_tag="VFA35_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56132.1"
                     /translation="MIECGSLEYTQARIGARHGERLREADWHRIEVLRELRPLLELAR
                     GTALRPWLAGIEADSSVHRVEAALRDRWRDLVAEVAGWMPARWRPALAWWAVLPDLAP
                     LQHLARGEEPAPWMRDDGAWRELCASAPEARAAQLAAGPLAALAAAWSAPQALPRAWQ
                     AEWRRRWPEQRREADDMLERLARAWLEHGRAFAAATSGQGWLLRGSLRAHLALLLRRT
                     TLHPAVAFIHLALCALDLERLRAELVRRIVFVRWAAA"
     gene            2206..3909
                     /locus_tag="VFA35_07905"
     CDS             2206..3909
                     /locus_tag="VFA35_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56133.1"
                     /translation="MLPLLAQYADLAARYRAYWPPAETCRPSAFPETPVAALQRSLTT
                     VRAWAAESEPVIQALQRDESERRELHLWQPLLETLGRRALDPGLLAGAGPWLGVRLFL
                     LPPDGESDPVAQIGAGALETLACTLEVGGATHLLVAGAPEALQALTQALTAHKGSAHA
                     VPAWLGADAARNQAHVSRRLGELEREIAELGATLASLNQRHGLPCALGDANRLQWVLQ
                     NVRALEAEELLCWITGWSSEPHVEAMGEALRACGARALPHYSAPPADAHAPLLLSNPR
                     WARPFEIFSRAFGMPSSSEADPSALLAIAVPLMFGYMFGDVGQGLVLAAAGGWFRKRY
                     PVARLLMVGGLAATAFGFLFGSVFSLHGLVPALWLHPLDAPLTLLLAPLAGGIVLLTV
                     GLGLNALEARWGGEMRRWLLTDAALIVVYLGLLAAWLTPVGLLAAAAGVLWFCIGHAV
                     LERRVVAAFGAVGEAIEKTLQILVNTLSFARVGAFALAHAGLSSAVVMLADAADNVVA
                     KALVLVLGNALVIVLEAMVVAIQTTRLVLFEFFARFMQGQGRAFRPLPAPPFVYQESS
                     R"
     gene            3906..4259
                     /locus_tag="VFA35_07910"
     CDS             3906..4259
                     /locus_tag="VFA35_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HZT56134.1"
                     /translation="MKRPPGAIALSLLGATLLLGLAATALLLWAPEARAAAGAAAPGG
                     LDASWLFIGAALATGMSSLGAGLAVAKVGTAAVGALAEKPELFGRLLIFIGLAEGIAI
                     YGLIVSILMLNRLGG"
     gene            4262..4552
                     /locus_tag="VFA35_07915"
     CDS             4262..4552
                     /locus_tag="VFA35_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HZT56135.1"
                     /translation="MPIYLGDEVSAAGYRLAGVRVRTPGPGEAATALAEACAQAPLVL
                     LSSAVAAGIGERPLRAALSALAPLVVVVPDLQGDVPVPDLAARLRAQLGLEA"
     gene            4549..>4735
                     /locus_tag="VFA35_07920"
     CDS             4549..>4735
                     /locus_tag="VFA35_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56136.1"
                     /translation="MTQAQAPLGVESAMQALLELVESNRARSCEQILGEAHVRAQALR
                     SQAYAGARTRMRQAFEEQ"
BASE COUNT          587 a         1711 c         1728 g          709 t
ORIGIN      
        1 caccgcatat tcccagcggc tgaaggtccg tcccaggcac acggccaggg tgcgcttgcc
       61 gacctgggcg tcgggatcga ggtcgcggta gttgttgacc accagcaccg ccgtggcagg
      121 aaagccgacc atggtcgcgg ctgtcagcgc gccgctgctg agcgacccgg tttgcaggta
      181 gtaggtgcca cccacggcca ccagaccgaa gaacagccag acgacgacat cgccgaacgg
      241 tgtgtaggag atcggccgcg cgccaccgga atagcccatc gctgcggccg cggcagcgat
      301 gcccgcgatc aggatcggcc agccgccccg ccaggccagc acgaggccga tcgcggtggc
      361 aagcgcgaag caggcacgcg atgccgagcg cacctggcca ggccccagca aacccagcgc
      421 cgtcgcgcgc ggcaggccga gccggccggc acggtcggtg ccgcgctctg agtcgctgac
      481 gtcgttgtcg aggttcgtgc ccgtttgaag cagcagggcg cccagcgccg agagcagcat
      541 cagcgggata tcgattcgtc cggtctcggc ccacgccagc gcgcaaccca cgagcaccgg
      601 gttgatgccg atggtcagcg tgtgcggacg cgatgccatg gcccatgccc gcaacgacgt
      661 tgcaaagcgc atccgcactg aactgtcgtg accgaccatg acctcatgga gtctagtcgc
      721 gtgccgccgc gttgaacggt cgcggcgcgg gttcggcgaa cctgaaaggc gataccgctt
      781 gctctcgagc gcaagctctc gcaaggcggc ggcgcggggg tcgcgacttg gccgcttccg
      841 ggcacgcctc tcggcctgcg cgtttgagat gggtcaagcc acgccgaccg gcctcggcgc
      901 aagatcatcc ggcgcggcag gggccgcagg ggggggtcgt gaagccgatt gctgacgacg
      961 aggcctacaa cgaagcggcc gaaggggcca tcgcgcgcgt gctcgtcgcc gagcgcgagg
     1021 cgcgcgaggc gatcgagcag gcgcggctcg aggtcgaccg gatcgtcgag cgtgcccgcc
     1081 tcggtgcccg agccctggac gaacgcaccg agcgccgcat ccgcgccgtg gtcgacgcgt
     1141 tcgaacgcga gctggccgag cggctggccg agatcgaagc cgccgccgag caggccgccc
     1201 gcccgcagcc gttctccgac gacgagttgg cagctttgca gcgtgcagtc cgtgcgctcg
     1261 ccacggagct catcatggct ccgccatgat cgagtgcggc agcctcgaat acacgcaggc
     1321 gcgcatcggc gcacgccacg gcgagcgcct gcgcgaggcc gactggcatc gcatcgaggt
     1381 gctgcgcgag ctcaggccgc tgctggagct ggcccgcggc accgcgctgc ggccctggct
     1441 cgcaggcatc gaggccgaca gcagcgtcca tcgggtcgaa gcagccctgc gcgaccgctg
     1501 gcgcgacctt gtcgccgagg tcgcgggctg gatgccggcc cgatggcggc cggcgctcgc
     1561 ctggtgggcg gttctgcccg atctggcgcc gctccagcat ctggcgcgcg gggaggagcc
     1621 ggctccatgg atgcgcgacg acggcgcgtg gcgcgagctt tgcgcgagcg cgcccgaagc
     1681 gcgtgccgcg cagctcgccg ccggaccgct cgctgccctg gcggccgctt ggtcggcgcc
     1741 gcaggcgctg ccgcgcgcct ggcaggcgga atggcggcgg cgctggcccg agcagcggcg
     1801 cgaggccgac gacatgctcg agcgtctggc acgcgcctgg ctcgagcatg gacgcgcgtt
     1861 cgccgccgcg acgagcgggc agggctggct gctgcgcggc tcgctgcggg cgcacctcgc
     1921 cttgctgctg cggcgcacca cgctccaccc ggccgtggct ttcatccatc tcgcgctgtg
     1981 cgcgctcgac ctcgagcgac tgcgcgccga gctcgtgcgg cgcatcgtgt tcgtgcgctg
     2041 ggcggcggcc taggcctcgt catgctgcga cctcgtcccg cccgctggtt cgagatccag
     2101 gccgcgcgcg acgacgcgac cctggtgctc gaggcgctgg cgcgcaccgg ggcagtcgag
     2161 ctcgaggcgc gcagcagcat cggcctgccg gcggcgttcg ccgacgtgct gccgctgctc
     2221 gcgcaatacg cggacctggc ggcgcgctat cgcgcctact ggccaccggc cgagacctgc
     2281 cggccctccg cctttcccga gacgccggtc gccgccctgc agcgcagtct gacgaccgtc
     2341 cgggcctggg cggcggagag cgagccggtg atccaggccc tgcagcgcga cgagtccgag
     2401 cgtcgcgagc tgcacctctg gcagcccttg ctcgagacgc tcggacggcg cgcgctcgac
     2461 cccggactgc tcgccggtgc cgggccctgg ctgggggtgc gcctgttcct gctgcctccc
     2521 gacggcgaat ccgatccggt agcccagatc ggcgcgggcg cgctcgagac gctggcttgc
     2581 accctggagg tcggcggcgc gacccacctg ctcgtggccg gggcgcccga ggcgctgcag
     2641 gcgttgaccc aggcgctcac ggcgcacaag ggcagtgcgc acgcggtgcc ggcctggctc
     2701 ggggccgatg ccgcgcgcaa ccaggcgcat gtgagccggc gcctgggtga actcgagcgc
     2761 gagatcgccg aactcggcgc gacgctcgcc tcgctgaacc agcgccatgg gctgccgtgc
     2821 gccctgggag acgcgaatcg cctgcaatgg gtgttgcaga acgtgcgcgc gctcgaagcg
     2881 gaggagttgc tgtgctggat caccggctgg tcgagcgagc cccacgtcga agcgatgggc
     2941 gaggcccttc gggcgtgcgg cgcgcgcgcg ctgccgcact acagcgcccc accggcggac
     3001 gcgcacgcgc cgctgctgct gagcaatccg cgctgggccc ggccgttcga gatcttcagc
     3061 cgcgccttcg gcatgccgtc ctcgagcgag gccgacccga gcgccctgct ggcgatcgcc
     3121 gtgcccctga tgttcggcta catgttcggc gacgtcggtc agggcctcgt gctcgccgcc
     3181 gccggcggct ggttccgcaa gcgatacccg gtggcccgcc tgctcatggt ggggggcttg
     3241 gcggcgaccg cattcggttt cctcttcggc agcgtcttca gcctgcacgg gctcgtgccg
     3301 gcgctttggc tgcatccgct cgatgcgccg ctgactctgc tgctggcacc tctggcgggc
     3361 ggcatcgtcc tgttgacggt gggactcggc ctgaacgcgc tcgaggcccg ctgggggggc
     3421 gagatgcgtc gctggctgct gacggacgcg gcgttgatcg tcgtctacct cggcctgctc
     3481 gcggcgtggc tcacgcccgt cggtctgctc gctgcggctg ccggcgtcct ctggttctgc
     3541 atcggtcatg cggtcctcga gcggcgcgtc gtggccgcct tcggtgccgt cggcgaggcg
     3601 atcgagaaga cgctgcagat cctcgtcaac accctttcgt tcgcgcgtgt cggcgcgttc
     3661 gcgctcgccc acgccggctt gtcgtcggcg gtcgtcatgc tggcagacgc ggcggacaac
     3721 gtcgttgcca aggccctggt cctggtcctc ggcaacgcgc tcgtgatcgt gctcgaggcg
     3781 atggtggtgg cgatccagac gacgcgtctc gttctgttcg aattcttcgc gcgcttcatg
     3841 caagggcagg ggcgcgcgtt ccgccccttg cccgctccgc cctttgtcta ccaggagagc
     3901 tctcgatgaa aagaccccca ggcgccattg cgctttcgtt gctcggtgcg actctcttgc
     3961 tggggcttgc agccacggcg ctgctgctgt gggcgcccga agcgcgcgct gccgccggcg
     4021 cggccgcgcc cggcgggctc gacgcgagct ggctcttcat cggcgccgcg ctcgcgaccg
     4081 gcatgtcgtc cctgggcgcc ggcctcgccg ttgccaaggt gggcactgcg gccgtgggcg
     4141 cgctggccga gaagcccgag cttttcggcc ggctcctgat cttcatcggc ctggccgagg
     4201 ggatcgcgat ctacgggctc atcgtgtcga tcctgatgct caaccggctc gggggatgac
     4261 ggtgcccatc tacctgggcg acgaggtcag cgcggccggc taccggctcg ccggggtacg
     4321 cgtgcgcacg cccgggccgg gcgaggccgc gaccgcgctt gccgaggcgt gcgcgcaggc
     4381 gccgctggtc ctcctctcct cggccgtggc cgccgggatc ggcgagcggc cgttgcgagc
     4441 cgccctgtcg gccctcgcgc cgctcgtcgt cgtcgttccc gatctgcagg gcgacgtccc
     4501 cgtgcccgat ctcgcggcgc gcctgcgcgc ccaactgggg ctcgaggcat gacgcaagcc
     4561 caggccccgc tcggcgtcga atcggcgatg caggccttgc tcgagctggt cgagtccaac
     4621 cgcgcgcgct cgtgcgagca gattctcggc gaggcccacg tccgtgcgca ggccctgcgc
     4681 agccaggcct acgccggcgc gcgcacgcgc atgcgccagg ctttcgagga acagc
//
LOCUS       DASRDG010000475         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1223531, whole genome shotgun sequence.
ACCESSION   DASRDG010000475 DASRDG010000000
VERSION     DASRDG010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223531"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..463)
                     /locus_tag="VFA35_07925"
     CDS             complement(23..463)
                     /locus_tag="VFA35_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HZT56137.1"
                     /translation="MIRAIAFFLVLVLVGVFALVNWGAFSALTTLSLGVTTVQAPLGL
                     IMLGLVAFLCVLFTVWVISLQAGSLAAARRQTRELQAQRDLADRAEASRFTELRTELW
                     ARLDRLQSDSRLAIEQNANSVAAQLGELEDRLERERLVKPVSQT"
     gene            complement(497..>2005)
                     /gene="acpA"
                     /locus_tag="VFA35_07930"
     CDS             complement(497..>2005)
                     /gene="acpA"
                     /locus_tag="VFA35_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HZT56138.1"
                     /translation="TVVVIYAENRAFDNLYGLFPGANGVPGKNPSSTGGFAPQRDFDG
                     SVLPVLPPAWGGLTAGGQPVTLTQAMSAGMPNAPFQIDDPKGLNGSGVVVGQNIITRD
                     LVHRYYSNQLQIAGGKNDGFAAWSDAGALSMGYYDGSAMKLWKLAQQYVLADNFFMGA
                     FGGSFLNHQYLICACMPVYPDADAPDSPAKDSITQITTDAQGRFTGFVPADNMPKTVL
                     AGAPRYKRDSTLTPKDANGVFYAVNTMQPAHQPSANAPAASGDPRLADRGKPTTLPPQ
                     TQPTIGDTLDAKQVPWAWYAGGWNTVESGSPEARKQIYSGKINFQAHHQPFNYYAALD
                     PATKSAARAAHLKDFDAAFLQDAAAGKLPAVAFYKPHGPLNQHAGYADVALGDAHIAE
                     VIDKLQRSPQWPRMLIVVTYDENGGFYDHAAVPRGDAAGPGTRIPALIVSPFAKRGFV
                     DKTQYDTGSILRFITHRWSLSPLPGIVMRDAGLARNGSAPMGDLSAALDFAR"
BASE COUNT          272 a          656 c          755 g          322 t
ORIGIN      
        1 gggaccgaac cctcgcccgg tgtcacgtct gagacacggg tttcaccagc cgctcgcgct
       61 cgaggcggtc ttcgagctcg ccgagttgcg cggcgaccga gttcgcgttc tgctcgatcg
      121 cgaggcgcga atcgctttgc agccggtcga gccgcgccca cagctcggtg cgcagctcgg
      181 tgaagcgcga ggcctcggcc cggtcggcga ggtcgcgctg ggcttgcagc tcgcgcgtct
      241 ggcgccgcgc cgcggcgagc gagccggcct gcagcgagat cacccagacc gtgaacagca
      301 cgcacaggaa cgcgaccagc ccgagcatga tcagcccgag cggcgcctgc accgtggtga
      361 cgccgaggct cagcgtcgtg agcgcggaga acgcgcccca gttgaccagc gcgaacacgc
      421 cgaccagcac gagcacgagg aagaaggcga tggcacgaat catgggcggt ctccttgcgg
      481 gtgagcagcc gggagcttag cgggcgaagt cgagggccgc gctgaggtcg cccatcggtg
      541 cgctgccgtt gcgcgccagg ccggcgtcgc gcatcacgat gccgggcagc gggctgagcg
      601 accagcggtg agtgatgaag cgcaagatcg agccggtgtc gtattgggtc ttgtcgacga
      661 agccgcgctt ggcgaacggc gagacgatca gcgccgggat gcgcgtgccc ggcccagccg
      721 cgtcgccgcg cggcaccgcg gcgtggtcgt agaagccgcc gttctcgtcg taggtgacga
      781 cgatcagcat gcgcggccac tgcgggctgc gctgcagctt gtcgatcacc tcggcaatgt
      841 gggcgtcgcc cagcgccacg tcggcgtagc cggcgtgctg gttcagcggg ccgtgcggct
      901 tgtagaacgc gacggcgggc agcttgccgg cggcggcgtc ctgcaggaac gcggcgtcga
      961 agtccttcag gtgtgcggcg cgcgcggcgg actttgtcgc cgggtcgagc gcggcgtagt
     1021 agttgaacgg ctggtggtgc gcctggaagt tgatcttgcc gctgtagatc tgcttgcgcg
     1081 cctcgggcga accgctctcg accgtgttcc agccgcccgc gtaccaggcc cacggcacct
     1141 gcttggcgtc gagcgtgtcg ccgatcgtcg gctgggtctg cggcggcagc gtggtcggct
     1201 tgccgcggtc ggccaggcgc gggtcgccgc tcgcggccgg cgcgttggcg ctgggctggt
     1261 gggccggctg catggtgttg acggcgtaaa acacgccgtt cgcgtccttc ggcgtcagcg
     1321 tgctgtcgcg cttgtagcgc ggcgcgccgg cgagcacggt cttcggcatg ttgtccgccg
     1381 gcacgaagcc ggtgaagcgg ccctgcgcgt cggtcgtgat ctgcgtgatc gagtctttcg
     1441 cgggcgagtc gggcgcgtcg gcgtccgggt agaccggcat gcaggcgcag atcaggtact
     1501 ggtggttcag gaacgagccg ccgaatgcgc ccatgaagaa gttgtcggcc agtacgtact
     1561 gctgcgcgag cttccacagc ttcatcgcgc tgccgtcgta gtagcccatg ctcaaggcgc
     1621 cggcgtcgga ccaggccgcg aagccgtcgt tcttgccgcc ggcgatctgc agctggttgc
     1681 tgtagtagcg gtgcaccagg tcgcgcgtga tgatgttctg gccgacgacc acgccgctgc
     1741 cgttcagccc cttcgggtcg tcgatctgga atggcgcgtt cggcatgccg gccgacatcg
     1801 cctgcgtcag cgtcaccggc tggccgcccg cggtcaggcc gccccacgcc ggcggcagca
     1861 ccggcagcac cgagccgtcg aagtcgcgct gcggcgcgaa gccgccggtc gagctggggt
     1921 tcttgccggg cacgccgttg gcgccgggga acaggccgta gaggttgtcg aacgcgcggt
     1981 tctcggcgta gatcacgacg accgt
//
LOCUS       DASRDG010000476         7403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_780058, whole genome shotgun sequence.
ACCESSION   DASRDG010000476 DASRDG010000000
VERSION     DASRDG010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780058"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFA35_07935"
     CDS             complement(<1..371)
                     /locus_tag="VFA35_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HZT56139.1"
                     /translation="MERDQASKFVNDLLRLMVSRNGSDLFLTADFPPAIKVDGKVTKV
                     SPQPLTGQHTLALTRSMMNDKQAAEFERTKECNFAVAPQGLGRFRVNAFVQQGNIGMV
                     MRTIPQTIPTIDSMDLPRVLK"
     gene            complement(382..1425)
                     /locus_tag="VFA35_07940"
     CDS             complement(382..1425)
                     /locus_tag="VFA35_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZT56140.1"
                     /translation="MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDH
                     KQVHGMVYDIMNDSQRKAYEETLECDFSFEIQGLARFRVNAFNQNRGAGAVFRTIPSK
                     ILTLEQLNAPKVFAELSLKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEFGHVLTVED
                     PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDAILVGELRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTIDRVVDVFPAAEKEMVRAMLSESLIGVISQTLCKTKDGSG
                     RIAAHEIMLGTAAIRNLIREAKVAQMYSSIQTGNAMGMQTLDQNLADLVRRNVVSPAE
                     ARGKAKFPENFPG"
     gene            1513..2232
                     /locus_tag="VFA35_07945"
     CDS             1513..2232
                     /locus_tag="VFA35_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZT56141.1"
                     /translation="MAMIANNVQQVHERVARACAQAGRPVQSVTLLAVSKTFGPEAVR
                     EAVAAGEHRFGENYVAEGVAKIAALAGARTPLGSALEWHLIGPLQSNKTRAVAEHFDW
                     VHSVDRLKTAQRLSEQRPAQLAPLQLCLQVNISGEASKRGLRTDELADVAHAVAALPR
                     VTLRGLMAIPEPAADVAAQRAPHGALRELLAQLQSDGLTQMDTLSMGMSADLEAAIAE
                     GATIVRVGSAIFGARAGGASG"
     gene            complement(2224..2697)
                     /locus_tag="VFA35_07950"
     CDS             complement(2224..2697)
                     /locus_tag="VFA35_07950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT56142.1"
                     /translation="MKIEIDDLSRPAIHALLNEHLRDMRAISPPESVHALDLDRLRRP
                     EITFWSAWDGEMLLGCGALMALDRRHGEVKSMRTPAARRRRGAGRALLTHIIDTARAR
                     GYERLSLETGATAAFAPAHQLYESAGFTRTGPFGDYTDDPNSVFMTLALAPRHSA"
     gene            complement(2694..3308)
                     /locus_tag="VFA35_07955"
     CDS             complement(2694..3308)
                     /locus_tag="VFA35_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZT56143.1"
                     /translation="MTESTQVENINRRIAHRVAALRAERGLSLDALAACSGVSRSMLS
                     LIERGETSATAVVLEKVAGGLGVALAALFEAERDAPGPLMRRAEQAQWRDPASGYLRR
                     NVSPPDHPSPLRIVEVEFPAGRRVVLEGASAVAAIEQQVWLLAGTMALTLGGQTWRLE
                     AGDCLALRVDGPIEFHNPGRHAARYAVVIASAAAQRVANRQVPR"
     gene            3380..4201
                     /gene="proC"
                     /locus_tag="VFA35_07960"
     CDS             3380..4201
                     /gene="proC"
                     /locus_tag="VFA35_07960"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZT56144.1"
                     /translation="MSKIASSLAFIGGGNMAAAIIGGLIKSGRAPGSILVVDPGEAQR
                     AKLQAGFGVRTFGAADTALNDAATVVWAVKPQFFQAAAAPCAAHVGGALQLSVMAGIR
                     SDAIAQATGAARVVRSMPNTPALIGQGIAGLFARAAVTAADRTEVEAVLAPTGRTLWV
                     AREEDLDAVTALSGSGPAYVFYFVEAMMQAAVEMGLSEAQGKELALATFAGATALARG
                     SDETPAVLRERVTSKGGTTYAALTSLEASGVKAAFVAALKAAQQRARELGDEFGT"
     gene            4363..5634
                     /locus_tag="VFA35_07965"
     CDS             4363..5634
                     /locus_tag="VFA35_07965"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZT56145.1"
                     /translation="MSYLSYVSQKGDTAWDTYISQVDRVLPYLGNLARWSETLKRPKR
                     ALIVDVPIELDNGSVAHFEGYRVQHNLSRGPGKGGVRYHPDVTLEEVMALSAWMTVKC
                     AAVNLPYGGAKGGIRVDPKLLSLKELERMTRRYTSEIGIIIGPQRDIPAPDVNTNGQI
                     MAWMMDTYSMNTGSTATGVVTGKPLHLGGSLGRVKATGRGVFVTGREAARRIGLNLEG
                     ARIAVQGMGNVGGSAAELFVQAGARLVAVQDHTGTLVNPNGFDIADTMATLKRDGGIG
                     NYTGAERIDNEAFWDTDCDILIPAALESQITAARANRIKAKLILEGANGPTLPDADDA
                     LHDRGILVVPDVICNAGGVTVSYFEWVQDFSSFFWSEDEINLRLDKIMVDALRKIWDT
                     ADRHKISLRTATFAVACERILIAREERGLYP"
     gene            complement(5638..6546)
                     /gene="ubiA"
                     /locus_tag="VFA35_07970"
     CDS             complement(5638..6546)
                     /gene="ubiA"
                     /locus_tag="VFA35_07970"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HZT56146.1"
                     /translation="MDLGLRRSRLALWLDLIRWDRPAGWLLLLWPTLSALWIAAGGFP
                     GWHLLGVFVAGTILMRSAGCAANDVADREFDRHVKRTAARPVTSGAVPPWEALTLGAA
                     LAVAAFALVLTTNAAAVAWSFAGLAVTVLYPFSKRFFAMPQAVLGVAFSFGIPMAFAA
                     VRGGEARTLADAFAAVPPLAWWLLFGNLFWVLAYDTEYAMVDRDDDLKIGIQTSAITL
                     GRFDVAGVMVFYGLFIAIWGVLGAQQGLGWPYFAGLAAAAAIALWHFTLIRDRSREGC
                     FRAFRLNHWVGLVVFAGVVVDHALRR"
     gene            6670..7089
                     /locus_tag="VFA35_07975"
     CDS             6670..7089
                     /locus_tag="VFA35_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56147.1"
                     /translation="MKKILAFALAAGCAGAAFAQTSVSIGINQPGVYGRITIGDVPPP
                     ALILPQPVVIERPAVVYQRAPIYLYVPPAHQQNWRQYCGRYNACNQPVYFVRDEWVRE
                     RYEHEHPGWNNGHHNGHDKHDESKGHGNNGKGHGHDK"
     gene            complement(7145..>7403)
                     /gene="prfC"
                     /locus_tag="VFA35_07980"
     CDS             complement(7145..>7403)
                     /gene="prfC"
                     /locus_tag="VFA35_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856265.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HZT56148.1"
                     /translation="KARILPARYNVARWVTCDEAAGGPKELQRFIDGNAHRVARDAVD
                     APCVLLEYAGELRAMQENWPKIRFHALREHAGLVFQKQLEG"
BASE COUNT         1178 a         2580 c         2565 g         1080 t
ORIGIN      
        1 tccttcagca cccgcggcag gtccatgctg tcgatcgtcg ggatcgtctg cgggatcgtc
       61 cgcatcacca tgccgatgtt gccctgctgc acgaaggcgt tgacgcgaaa ccggcccagg
      121 ccctgcggcg cgaccgcgaa gttgcactcc ttggtgcgtt cgaactcggc cgcctgcttg
      181 tcgttcatca tcgaacgcgt cagtgccagc gtgtgctggc cggtcagcgg ctgcggcgaa
      241 accttcgtga ccttgccgtc aaccttgatc gcgggcggaa agtcggcggt gagaaacagg
      301 tcggagccgt tgcgcgacac catcagccgc agcaggtcgt tgacgaattt cgatgcctga
      361 tcgcgttcca tttggctttc cttaccccgg gaagttttcc gggaatttgg ccttgccgcg
      421 cgcctcggcc ggcgatacca cgttgcgtcg caccaggtcg gcgaggttct gatcgagcgt
      481 ctgcatgccc atcgcgttgc cggtctggat cgacgaatac atctgcgcga ccttggcctc
      541 gcggatcagg ttgcggatcg ccgcggtgcc cagcatgatc tcgtgcgccg cgatccggcc
      601 gctgccgtcc ttggtcttgc acaaggtctg cgagatcacg ccaatcagcg actccgacag
      661 catcgcgcga accatctcct tctcggccgc cgggaatacg tcgaccacgc ggtcgatcgt
      721 cttggccgcg ctcgaggtgt gcaaggtgcc gaacaccagg tggccggttt cggcggcggt
      781 cagcgccagg cggatggttt cgaggtcgcg caactcgccc accaggatcg cgtccgggtc
      841 ttcgcgcagc gcgctgcgca gcgcgttgtt gaaactcagc gtgtgcggcc cgacctcacg
      901 ctggttgatc aggcacttct tcgattcgtg cacgaactcg atcgggtcct cgaccgtcag
      961 cacgtggccg aactcgttct cgttcaggtg gttgaccatc gcggcgagcg tggtcgactt
     1021 gcccgagccg gtcggcccgg tcacgagcac gaggccgcgt ggcttcagcg acagctcggc
     1081 gaagaccttc ggtgcgttca gctgctcgag cgtcaggatc ttcgacggaa tcgtccggaa
     1141 caccgcgccg gcgccgcggt tctggttgaa ggcgttgacg cgaaagcgcg ccaggccctg
     1201 gatctcgaac gagaagtcgc actccagcgt ctcttcgtac gccttgcgct gcgagtcgtt
     1261 catgatgtcg tacaccatgc cgtggacctg cttgtggtcc agcgggtcga cgttgatgcg
     1321 ccgcacgtcg ccatgcaccc ggatcatcgg cggcagcccg gccgagaggt gcaggtccga
     1381 ggccttgttc ttgaccgaaa acgccagcag ttgggtgatg tccatggggc cttgggtggg
     1441 tgggcgtcgg gccgcgcggt gatgtcccgc gccccgcgac gcgcgctggc gctgcgggat
     1501 actcggcgca ttatggcgat gatcgcaaac aacgtacaac aagtgcacga gcgcgttgcc
     1561 cgggcctgcg cgcaggccgg gcgccccgtg caaagcgtca cgctgctggc cgtcagcaag
     1621 accttcggcc ccgaggcggt gcgcgaagcc gtggcggccg gggaacaccg cttcggcgaa
     1681 aactacgtgg ccgaaggcgt cgcgaagatc gccgcgctgg ccggtgcgcg cacgccgctc
     1741 ggcagcgcgc tcgaatggca cctgatcggc ccgctgcagt cgaacaagac acgcgcggtc
     1801 gccgagcact tcgactgggt gcattcggtc gaccggctga agaccgccca gcgcctgagc
     1861 gagcagcggc cagcgcagct cgcaccgctg cagctgtgtc tgcaggtcaa catcagcggc
     1921 gaggcgagca agcgcggctt gcggaccgac gagcttgccg acgtggccca tgcggtggcc
     1981 gcgttgccgc gcgtcacgct gcgcgggttg atggcgatcc ccgaacccgc cgccgatgtc
     2041 gccgcgcagc gcgcaccgca cggcgcgctg cgcgagttgc tcgcgcagtt gcagtccgac
     2101 ggcctgacgc agatggacac gctgtcgatg ggcatgagcg ccgatctcga agcggccatc
     2161 gccgagggcg cgacgatcgt gcgcgtgggc agcgcgatct tcggggcgcg cgccggcggc
     2221 gcgtcaggct gagtgtcgcg gtgccagcgc cagcgtcatg aagacgctgt tcgggtcgtc
     2281 ggtgtagtcg ccgaacgggc cggtgcgcgt gaagccggcg ctctcgtaca gctggtgcgc
     2341 gggcgcgaac gcggccgtcg ccccggtctc caggctcagg cgctcgtagc cgcgagcgcg
     2401 tgccgtgtcg atgatgtgcg tcagcagtgc ccggcccgcg ccccgtcggc gccgcgccgc
     2461 gggcgtgcgc atcgacttta cctcgccgtg ccggcggtcc agcgccatca gcgcgccaca
     2521 gccgagcagc atctcgccat cccaggccga ccagaaggtg atctcgggcc ggcgcagccg
     2581 gtcgaggtcg agcgcgtgca cgctctcggg cggcgagatc gcgcgcatgt cccgcaggtg
     2641 ctcgttcagc agcgcgtgga tggccgggcg ggagaggtcg tcgatctcga tcttcatcgg
     2701 ggcacctgtc ggttcgcgac ccgctgcgcc gctgcgctcg cgatcacgac cgcgtagcgc
     2761 gccgcgtggc gaccggggtt gtggaattcg atcggcccat cgacgcgcag cgccaggcag
     2821 tcgccggcct cgagccgcca ggtctggccg cccagcgtca gcgccatcgt gccggccagc
     2881 agccagacct gctgctcgat cgccgccacg gcactcgcgc cttcgagcac gacgcgccga
     2941 ccggccggga actcgacctc gacgatgcgc agcggcgacg ggtgatcggg cggcgagacg
     3001 ttgcgccgca ggtagcccga ggccgggtcg cgccactgcg cttgctcggc gcgccgcatc
     3061 agcgggccgg gcgcgtcgcg ttcggcctcg aacaacgcgg ccagcgcgac gcccagcccg
     3121 ccggccacct tctcgagcac gaccgcggtc gcgctcgtct cgccgcgctc gatcagcgac
     3181 agcatcgagc ggctcacccc gctgcacgcg gccagcgcgt cgagcgacag gccgcgctcg
     3241 gcgcgcagcg cggcgacgcg gtgggcgatg cgccggttga tgttttcgac ctgggtagat
     3301 tccgtcatga tggaatcatt ctccattatt atggaatatc tcgtcaaacg tcacgtcgga
     3361 tggcgcataa actcgtcgca tgagcaagat cgcatcctca ctggcgttca tcggcggcgg
     3421 aaacatggcc gccgccatca tcggcggcct gatcaagagc ggccgcgcac ccggttcgat
     3481 tctcgtcgtc gaccccggcg aggcgcagcg cgcgaagctg caggccgggt tcggcgtgcg
     3541 caccttcggc gccgccgata ccgcgctgaa cgatgccgcg acggtcgtct gggccgtcaa
     3601 gccgcagttc ttccaggccg cggcggcgcc gtgcgcggcg catgtcggcg gcgcgctgca
     3661 gctcagcgtg atggccggta tccgcagtga cgcgatcgcg caggcgaccg gcgcggcgcg
     3721 cgtggtgcgc tcgatgccga acacgccggc gctgatcggc cagggcatcg ccgggctgtt
     3781 cgcgcgcgcc gcggtgactg cggccgatcg caccgaggtc gaagccgtgc tcgcgccgac
     3841 cggccgcacg ctctgggtcg cgcgcgaaga agacctggac gccgtgaccg cgctgtcggg
     3901 ctccggtccg gcctatgtct tctacttcgt cgaggcgatg atgcaggccg ccgtcgagat
     3961 gggcctgtcg gaggcgcaag gcaaggaact cgcgctcgcc accttcgccg gcgcgaccgc
     4021 gctcgcgcga ggctccgacg agacccccgc ggtgctgcgc gagcgcgtca cgtccaaggg
     4081 cggcacgacc tacgcggcgc tcacctcgct cgaagccagc ggcgtgaagg cggcgttcgt
     4141 tgccgcgctg aaggcggcgc aacagcgcgc gcgcgaactc ggcgacgagt tcggcacctg
     4201 aagacgcccg gcgcaatgcc gggttcgggt gaccgcatgc aaagcccgca tgagggccgg
     4261 gcgggttttt ccgcgacggc gggtctacag tctcgggcca tgcaggccgg tgcgccacga
     4321 gcgcgccgcg ccggagcatg aagtcaaacc accgagacaa ccatgagcta cctctcctac
     4381 gtcagccaga agggcgacac cgcctgggac acctacatca gccaagtcga ccgcgtgctg
     4441 ccctacctgg gcaacctcgc gcgctggtcc gagacgctga agcgcccgaa gcgcgcactg
     4501 atcgtcgacg tgccgatcga gctcgacaac ggcagcgtcg cccacttcga gggctaccgc
     4561 gtgcagcaca acctctcgcg cgggccgggc aagggcggcg tgcgctacca ccccgacgtg
     4621 acgctcgaag aggtgatggc gctctcggca tggatgacgg tcaagtgcgc cgcggtgaac
     4681 ctgccctacg gcggcgccaa gggcggcatc cgcgtcgacc cgaagctgct ctcgctgaag
     4741 gagcttgagc gcatgacgcg ccgctacacc agcgagatcg gcatcatcat cggcccgcag
     4801 cgcgacatcc ccgcgcccga cgtcaacacc aacggccaga tcatggcctg gatgatggac
     4861 acgtactcga tgaacaccgg cagcaccgcc accggcgtcg tcaccggcaa gccgctgcac
     4921 ctgggcggct cgctgggccg cgtcaaggcc accggccgcg gcgtgttcgt gaccggccgc
     4981 gaagcggcgc ggcgcatcgg gctgaacttg gagggcgcgc gcatcgcggt gcagggcatg
     5041 ggcaacgtcg gcggctcggc ggccgagctc ttcgtgcagg ccggcgcccg gctggtcgcg
     5101 gtgcaggacc acaccggcac gctcgtgaac ccgaacggct tcgacatcgc cgacacgatg
     5161 gccacgctca agcgcgacgg cggcatcggc aactacaccg gcgccgagcg catcgacaac
     5221 gaggcgttct gggacacaga ctgcgacatc ctgatcccgg ccgcactcga gagccagatc
     5281 accgcggcgc gcgcgaaccg catcaaggcc aagctgatcc tggagggcgc gaacggcccg
     5341 accctgcccg acgccgacga cgcgctgcac gaccgcggca tcctggtcgt gcccgacgtg
     5401 atctgcaatg ccggcggcgt gacggtcagc tacttcgagt gggtgcagga cttctcgtcg
     5461 ttcttctgga gcgaggacga gatcaacctg cgcctggaca agatcatggt cgacgcgctg
     5521 cgcaagatct gggacaccgc ggatcgccac aagatcagcc tgcgcaccgc gaccttcgcg
     5581 gtggcatgcg agcgcatcct catcgcgcgc gaagagcgcg gtttgtatcc ctgatcgtca
     5641 gcgacgcagc gcgtggtcga cgaccacgcc tgcgaacacg acaagaccga cccagtgatt
     5701 gagccggaac gcgcgaaagc agccctcgcg cgagcggtcc cggatcagcg tgaagtgcca
     5761 cagcgcgatc gccgccgcgg ccgcgagccc ggcgaagtag ggccagccca ggccctgctg
     5821 cgcgccgagc acgccccaga tcgcgatgaa cagaccgtag aacaccatca cgccggccac
     5881 gtcgaagcgg ccgagcgtga tcgccgaggt ctggatgccg atcttcaggt cgtcgtcgcg
     5941 gtccaccatc gcgtactcgg tgtcgtaggc gagcacccag aacaggttgc cgaacagcag
     6001 ccaccaggcc agcggcggca cggcggcgaa cgcgtcggcg agcgtgcgcg cctcgccgcc
     6061 gcgcaccgcg gcgaacgcca tcggaatgcc gaagctgaag gcgacgccga ggaccgcctg
     6121 cggcatcgcg aagaagcgct tgctgaacgg atagagaacc gtcacggcca ggccggcgaa
     6181 actccaggcg acggccgcgg cattggtcgt gagcacgagt gcgaacgcgg ccacggcgag
     6241 cgccgcgccg agcgtgagcg cttcccacgg cgggaccgcg ccgctggtca ccggccgcgc
     6301 ggcggtgcgc ttgacgtggc ggtcgaactc gcgatcggcg acgtcgttgg cggcgcagcc
     6361 ggcgctgcgc atcaggatcg ttccggcgac gaacaccccc agcaggtgcc agcccgggaa
     6421 gccgccagcg gcgatccaga gcgccgagag cgtgggccag agcagcagca gccagccggc
     6481 gggccggtcc cagcggatca ggtcgagcca gagcgcgagg cgcgatcgcc gcaggccgag
     6541 gtccattgct tttcgttact accgttcggc ggtcacggcg tgaagtccga cacggacccg
     6601 cgctcgaacc cggtctcggg cccgtcgtcg cgatgcgtta atgaaagaca ccttgtccag
     6661 gagacacgca tgaagaagat tctcgctttt gcactggccg ccggctgcgc cggtgcggca
     6721 ttcgcccaga ccagcgtctc gatcgggatc aaccagccgg gcgtctacgg ccgcatcacc
     6781 atcggcgacg tccctcctcc ggcgttgatc ctgccgcagc cggtggtcat cgaacgcccg
     6841 gccgtggtgt accagcgggc gcccatctac ctctacgtgc cgccggcgca ccagcagaac
     6901 tggcgccagt actgcgggcg ctacaacgcc tgcaaccagc cggtctactt cgtgcgcgac
     6961 gagtgggtgc gggagcgcta cgagcacgag catccgggct ggaacaacgg ccaccacaac
     7021 ggccacgaca agcacgacga gtccaagggc cacggcaaca atggcaaggg ccacggccac
     7081 gacaagtagc gccgcacggc cgggtcagag ccacccggcc acgtcgcgcc gcggctgacg
     7141 cggctcagcc ctcgagctgc ttctggaaca ccagcccggc gtgctcgcgc agggcgtgga
     7201 agcggatctt cggccagttc tcctgcatcg cccgcagctc gccggcgtac tcgagcagga
     7261 cgcagggcgc gtcgaccgcg tcgcgggcga cgcggtgggc gttgccgtcg atgaagcgct
     7321 gcagctcttt cggtccgccg gcggcctcgt cgcaggtcac ccagcgggcg acgttgtagc
     7381 gcgccggcag gatccgcgcc ttg
//
LOCUS       DASRDG010000477         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1223818, whole genome shotgun sequence.
ACCESSION   DASRDG010000477 DASRDG010000000
VERSION     DASRDG010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223818"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1420
                     /gene="priA"
                     /locus_tag="VFA35_07985"
     CDS             <1..1420
                     /gene="priA"
                     /locus_tag="VFA35_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZT56149.1"
                     /translation="SALTPAQRLRHWLAAHLGLADLVLGTRLAVFASMPRLGLIVVDE
                     EHDPSYKQQEGARYSARDLAVYRGRLERATVVLGSATPSLESWQRAVEGKYLKLALPA
                     RIGGGALPSVRLVDMNQQPKRHGANTALSAPLVAALQDRLARGEQSLVFLNRRGYAPV
                     LHCGECGWKSGCPHCSAWRVFHKLDRTLRCHHCGFSERVPRACPDCGNLDIAPIGRGT
                     ERLEEQLAALLRTPDDRPARIARIDADSTRLKGALEAQLGAVHAGDVDVLVGTQMVTK
                     GHDFRRITLVAAVNPDSSLFSSDFRAPERLFALLMQAGGRAGRDARQAERSELWVQTW
                     NPTHALYGALRRHDFAAFASSQLKEREMAGLPPFSHLAMLRADARTPEAARAFLHAAA
                     ALAATLPGADQVMVYPPVPMSVARVADVERVQMLVESASRVALQRFLARWLPALHTLR
                     RAHKHADERILRWAIDVDPSAI"
     gene            complement(1409..2146)
                     /locus_tag="VFA35_07990"
     CDS             complement(1409..2146)
                     /locus_tag="VFA35_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HZT56150.1"
                     /translation="MSGPHQPPRPRNAPRPPRPAAPARSVRPLNERELDELQALLDRV
                     PAPLEALDVSMLDGYLCGVLLQPRHIAVARWLPHVIDVDGRAPPPSFDAARLGSLVLR
                     RHAELDDAIQRRQWFDPWVFELSNDDETVGEDDEDDEGSGAVDAVYPWVAGFVTALEL
                     FPDLMAQDAKALTEPLALVYRHLAPDDLEDADELLAEIESLEPPEDLADAVEGLVRAT
                     LLLADISRPVVTAAAAPMRPRRAPSNR"
     gene            2689..3423
                     /locus_tag="VFA35_07995"
     CDS             2689..3423
                     /locus_tag="VFA35_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56151.1"
                     /translation="MKNTLTTLAVAAIVTACGGGGGNDSPSGASTAQGLYIGSTSTGR
                     AVTGLVLSDGTTYVLYSSASSPSVIAGVVQGTVGTTASDFRDQDARDFNLEGFGVLAA
                     TVAGTYVTKRSVSGTIFYAKSSVAFTGTYSSDYELKPSLSTIAGSYSGQVASSAGTQT
                     AAFTISSTGAVAGSSGGCAATGQFTPRADGNAYDTTVTFGAAPCLFAGQTLTGIAYFN
                     VATKRLYAAAPNAARTDGVLFVGTKP"
     gene            3420..>4132
                     /locus_tag="VFA35_08000"
     CDS             3420..>4132
                     /locus_tag="VFA35_08000"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HZT56152.1"
                     /translation="MKPIAAERPSSTTFTYRRRHPMKLPRVCAALMLAAVLTGCAVNV
                     TRSDGGFTPLPIGAATPAIAKHLALRMSGQQGGDWAKFTTEWREAIGDQAATSGMSFE
                     YLGAIAAPTATVGTLLEVKISDYRYVSPAARFLLGIMTGNAFLDAQVSFIDLATGHEI
                     GNKRYNTTSSAWEGIASPMSDRQIRAVASQIMGDLYAARPANSVPRTVAAKPTNEPQG
                     KDAFAAERLAKAQSCNASP"
BASE COUNT          660 a         1458 c         1400 g          614 t
ORIGIN      
        1 cagcgcgctg acgccggcac agcgcctgcg ccattggctc gccgcacacc tggggcttgc
       61 cgatctcgtg ctcggcacgc gcctcgcggt gttcgcgtcg atgccgcggc tcgggctgat
      121 cgtcgtcgac gaggagcacg acccgtcgta caagcagcag gaaggcgcgc gctactcggc
      181 gcgcgacctg gcggtgtacc gcggccggct cgagcgcgcg accgtcgtgc tcggctcggc
      241 gacgccgtcg ctcgagagct ggcagcgcgc cgtcgagggc aagtacctga agctcgcgct
      301 gccggcgcgc atcggcggcg gcgcgctgcc aagcgtgcgg ctggtcgaca tgaaccagca
      361 gccgaagcgc cacggcgcga acacggcgct gtcggcaccg ctggtcgccg cgctacagga
      421 ccggctcgcg cgcggcgagc aaagcctcgt cttcctgaac cgccgcggct acgcgccggt
      481 gctgcattgc ggcgagtgcg gctggaagag cggctgcccg cattgcagcg cctggcgcgt
      541 gttccacaag ctcgaccgca cgctgcgctg ccaccactgc ggtttcagcg agcgcgtgcc
      601 acgcgcctgc cccgattgcg gcaacctcga catcgcgccg atcggccgcg gcaccgaacg
      661 cctcgaggag cagctcgccg cactgctgcg cacgcccgac gaccggccgg cgcgcatcgc
      721 ccgcatcgac gccgacagca cccggctcaa gggcgcgctc gaagcgcagc tcggcgcggt
      781 gcatgccggc gatgtcgacg tgctggtcgg cacccagatg gtgaccaagg gccacgactt
      841 tcgccgcatc acgctggtcg ccgcggtgaa ccccgacagc tcgctgttca gcagcgactt
      901 ccgcgcgccc gagcgcctgt tcgcgctgct gatgcaggcg ggcggccgcg ccgggcgcga
      961 cgcgcgccaa gccgagcgca gcgagttgtg ggtgcagacc tggaacccga cgcacgcgct
     1021 ctacggcgcg ctgcgccggc acgacttcgc ggccttcgcg agcagccagc tgaaggagcg
     1081 cgagatggcc ggcctgcccc cgttctcgca cctcgcgatg ctgcgcgccg atgcgcgcac
     1141 gccggaggcc gcgcgcgcgt tcctgcacgc cgcggccgcg ctcgcggcga cgctgcccgg
     1201 cgccgatcag gtgatggtct acccgcccgt gccgatgagc gtagcgcgcg ttgccgatgt
     1261 cgagcgcgtg cagatgctgg tcgagtcggc ctcgcgcgtt gcgctgcagc gcttcctggc
     1321 acgctggctg ccggcgctgc acacgctgcg gcgcgcgcac aagcacgccg acgagcgcat
     1381 cctgcggtgg gccatcgacg tcgacccgtc agcgatttga cggcgcgcgg cgcgggcgca
     1441 tcggcgccgc cgccgcggta acgacgggcc gcgagatgtc ggccagcaag agcgtcgcgc
     1501 gcaccaggcc ttcgaccgca tcggccaggt cttcgggcgg ctcgagcgat tcgatctcgg
     1561 cgagcagttc gtcggcgtct tcgaggtcgt cgggcgcgag gtggcggtag accagcgcga
     1621 gcggctcggt cagcgccttc gcgtcctgcg ccatcagatc ggggaagagt tcgagcgcgg
     1681 tcacgaagcc ggcgacccac gggtagaccg cgtcgaccgc acccgagcct tcatcgtctt
     1741 cgtcgtcctc tccgacggtc tcgtcgtcat tgctcagctc gaacacccac gggtcgaacc
     1801 actggcgccg ctggatcgcg tcgtcgagct cggcgtggcg gcgcagcacc agcgaaccga
     1861 gccgcgcggc gtcgaagctc ggcggcggcg cgcggccgtc gacatcgatc acgtgcggca
     1921 gccagcgcgc gacggcaata tgccgcggct gcagcagcac gccgcagagg tagccgtcga
     1981 gcatgctcac gtcgagcgcc tcgagcggcg ccggcacgcg gtcgagcagc gcttgcaatt
     2041 cgtcgagttc gcgctcgttg agcggccgga cgctgcgcgc cggcgcggcc gggcgtggcg
     2101 gacgcggcgc gttgcgcggt cttggaggtt ggtgcgggcc ggacatggcg cattgtcgcg
     2161 cgcttggcgc ggcgcatgcg cgcgacagca cagcgcgcgc ggtgtgacgg atcacgcacc
     2221 gatgcggcgt ttcgccacat cccttgccac cttccgcacg cagccctcga attggggatg
     2281 gcggccttgg gggcgcatcc ctagagtcgt cccgattgct gtcacacggc gcctcgcgag
     2341 aggcacgctg caaggttcga aggcccgacg aagaccaccc acaccgaaca gggcaacgaa
     2401 ccgctaccgg tcccaacgac gtcctttccg aaggacttcc aagcccaccc gctcacgcgg
     2461 gtgggctttt tttttcacgg tgcgcgtgac gaaccgatgc ttggggcgga gccggcgagg
     2521 cggcgaactc gccgtgaagc ggcttgggcg tgatgctggt cactggtggc tgtcgtcagg
     2581 caaccccgaa cagcgtcgaa atcgccgcaa ggcgtgtgac gatgcggtcg ttctgctcgg
     2641 ccgagtcggg cacaagcaga gcgaacacat ttggcaaagg gagcaaggat gaagaacaca
     2701 ctgaccactc ttgcagtggc cgcaatcgtc actgcctgcg gtggcggcgg gggcaacgac
     2761 agccccagcg gagcctcgac cgcccaaggt ctgtacatcg gttcgacgag caccggccgg
     2821 gcggtcaccg gcttggtatt gagcgacgga acgacctacg tgctctactc atccgccagc
     2881 agtccgagcg tgattgcggg cgtggtgcaa ggcacggtcg gcacgaccgc ctcggatttc
     2941 cgcgaccagg acgcccgcga tttcaacctt gaaggcttcg gcgtcttggc agctaccgtc
     3001 gccggcacat acgtgacgaa gaggtcggtc agcggcacga tcttctatgc caagagctcg
     3061 gtcgccttca ctggaacgta cagctcggac tacgagttga aacccagcct ctcgaccatc
     3121 gccggtagct attctggaca ggtcgcctcc tccgccggca cgcagacggc ggccttcaca
     3181 atctcgagta ccggcgcggt tgcaggatcg tccggcggtt gcgcagcgac cggccagttc
     3241 acgccgcgcg ccgatggcaa cgcctacgac accaccgtga cgttcggtgc cgcgccatgc
     3301 cttttcgccg gccagaccct caccggcatc gcgtacttca acgtggcgac caagcgcctg
     3361 tatgccgcag cgcccaacgc ggccagaaca gacggcgtgc tgttcgtcgg gacgaagcca
     3421 tgaaaccgat cgcggccgaa cgaccgagca gcaccacctt cacctaccga agacgccatc
     3481 cgatgaaact gcctcgtgtt tgtgctgccc tcatgctcgc ggcagtcctg accgggtgtg
     3541 ccgtgaacgt cacgcgcagc gacggcggct tcaccccgct tccgatcggg gcggcgacgc
     3601 cggccatcgc caagcacctg gcacttcgga tgagcggcca gcagggcggc gactgggcca
     3661 agttcacgac agagtggcgc gaagccatcg gcgaccaggc ggcgacatcg gggatgagct
     3721 tcgagtacct cggtgcaatt gccgcaccca ctgccaccgt cggcacgttg ttggaggtca
     3781 agattagcga ctaccggtac gtcagccctg ccgctcggtt tctcctcggc atcatgaccg
     3841 gcaacgcgtt cctggacgcc caggtttcgt tcatcgatct cgcgaccggt cacgagatcg
     3901 ggaacaagcg atacaacacc acatcctcgg cttgggaggg catcgcctca ccgatgtccg
     3961 atcgccagat ccgcgcggtc gcgtctcaga tcatgggcga cctgtatgcc gccaggccgg
     4021 cgaactcggt gcctcgcacc gtcgcagcaa agccgacaaa cgagccccaa ggcaaggatg
     4081 cctttgccgc agaacggctt gccaaggcac agtcgtgcaa cgcctctccc ca
//
LOCUS       DASRDG010000478         4072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_997465, whole genome shotgun sequence.
ACCESSION   DASRDG010000478 DASRDG010000000
VERSION     DASRDG010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997465"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFA35_08005"
     CDS             <1..333
                     /locus_tag="VFA35_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HZT56153.1"
                     /translation="ITLLPDASITAAHDNPHFALAFARIENHYFVHRGFFDEGQLLRD
                     APRLAAIPGVIVQGRYDACTPVKTAWDLHRAWPQAEFHLVPDAGHAFKEPGILDRLIR
                     ATDRFAST"
     gene            complement(350..538)
                     /locus_tag="VFA35_08010"
     CDS             complement(350..538)
                     /locus_tag="VFA35_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56154.1"
                     /translation="MTTTKSLFVAALLSAFAAASFAQAPTPPAAPQQHGARTHQLHKH
                     HKHHHRHHHHAHKAHAPR"
     gene            complement(689..1216)
                     /locus_tag="VFA35_08015"
     CDS             complement(689..1216)
                     /locus_tag="VFA35_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56155.1"
                     /translation="MSNHRFPLTLKTALAVACASLALVAGGADAQGRGARGGGAGHGY
                     SGGGHSDHGGGWHRGGGGWIWGGLGLGLGLGLASYYASPWYYGYADPGYVVVNPPTVV
                     YEGAPPSAYGQPVPARSSQPVIYPRNGQGAAQTDADANACSEWAGRQPNATRDPSVFM
                     RGTQACMDARGYTVR"
     gene            1381..1848
                     /locus_tag="VFA35_08020"
     CDS             1381..1848
                     /locus_tag="VFA35_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZT56156.1"
                     /translation="MSAILDTSPLLSILDAARAKARAINLPFAGGVRPRDAYALTHAG
                     FATLVDVRSAEERRFVGHVPDSAHVAWATGTALTRNPRFVRELEAKLRKDSVVLLLCR
                     SGKRSALAAEAATKAGFTAAFNVLEGFEGELDANQHRGGADGWRFHALPWIQD"
     gene            1864..2814
                     /gene="epsC"
                     /locus_tag="VFA35_08025"
     CDS             1864..2814
                     /gene="epsC"
                     /locus_tag="VFA35_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HZT56157.1"
                     /translation="MSLDIPIVVAQLREERSRWRALQKRWRDPGGRELPAREALAQIV
                     QGLRGALFPMRLGPGDLRQESEDYYVGHTLDIALNALLAQVRLELRHEARQQPGFTAG
                     DADDARIEAGFDARATELVREFALSLAAIRALLDTDVQAAFHGDPAARSVDEVLLCYP
                     GIEAMIHHRLAHRLYALGVPLIARIVAELAHAATGIDIHPGATIGAGFFIDHGTGVVI
                     GETAVIGARVRVYQAVTLGAKRFPADAQGHLQKGLPRHPVVEDDVVIYAGATVLGRVT
                     LGAGSTIGGNVWLTHSVPPGSHVTQAVPQGDGADAPALRA"
     gene            2876..3802
                     /locus_tag="VFA35_08030"
     CDS             2876..3802
                     /locus_tag="VFA35_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HZT56158.1"
                     /translation="MTDPAQQALSDLGARQLADATKTAPKLDTISPRWLTHLLQWLPV
                     EAGIYRLNKVKNPEGVLVACTNRGDESELPSTFVDYEDTPREYFLNGVSTVLDVHTRV
                     SDLYNSPFDQIKEQLRLTIETIKEKQESELINNPDYGLLASVADSQIVYPLTGAPTPD
                     DFDELLTKVWKEPALFLAHPLAIAAFGRECTRRGVPPPTVSLFGSQFITWRGVPLIPS
                     NKVPVADGKTKVLLLRVGDKRQGVVGLYQPGLAGEQSPGLSVRFMGINRHAIASYLVS
                     LYCSLAVQTTDALAVLDDVEIGKYHDYPDTYK"
     gene            3777..>4072
                     /locus_tag="VFA35_08035"
     CDS             3777..>4072
                     /locus_tag="VFA35_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZT56159.1"
                     /translation="MTIQTPTSDLSGPLDAAALNQLAGAFFAALPGQAPIGAQAPANP
                     LPAGATIGPFGNGPPLPGTLVPGGNALPQSPQDIASIAAAQPARFPKVEAGNGVP"
BASE COUNT          583 a         1494 c         1432 g          563 t
ORIGIN      
        1 atcacgctgc tgcccgacgc gtcgatcacg gcggcgcacg acaacccgca tttcgcgctc
       61 gcgttcgcgc gcatcgagaa ccactacttc gtgcaccgcg gcttcttcga cgagggccag
      121 ctgctgcgcg acgcgccgcg gctcgccgcg atccccggcg tgatcgtgca gggtcgctac
      181 gacgcctgca cgccggtgaa gacggcgtgg gacctgcacc gcgcgtggcc gcaggccgag
      241 ttccacctcg tcccggatgc cggccacgcg ttcaaggagc ccggcatcct cgaccggctg
      301 atccgcgcga ccgaccggtt cgcgagcacc tgaccggccg cgatttcggt cagcgcggcg
      361 cgtgcgcctt gtgcgcgtgg tggtggtgac gatggtgatg cttgtggtgc ttgtgcagct
      421 ggtgcgtgcg cgcaccgtgc tgctgcgggg cggcgggcgg cgtgggcgcc tgcgcgaacg
      481 aggcggcggc aaacgccgac agcaacgcgg cgacgaagag cgacttggtg gtggtcatgg
      541 tggtgatgcg tccgtggtca accggattcg atgtggctcc gaatccttcg agcggacccg
      601 gcatgcgccg ggcttgtgac cacaacgggc gcggcacgcg ccgggccgac ccgtgcggac
      661 gcaaagaatt gctgcgcgaa ccggggcttc agcgcaccgt gtagccgcgc gcgtccatgc
      721 aggcttgggt gccgcgcatg aacacgctcg ggtcgcgcgt cgcgttcggc tgccggccgg
      781 cccactcgct gcaggcgttg gcgtcggcat cggtctgcgc ggcgccctgg ccgttgcgcg
      841 ggtagatcac cggctgcgac gagcgcgccg gcaccggctg gccgtaggcg ctgggcggcg
      901 cgccctcgta gaccaccgtc ggcgggttca cgaccacgta gccggggtcg gcgtagccgt
      961 agtaccaggg cgacgcgtag tagctcgcca gcccgaggcc caggcccagc ccgaggccgc
     1021 cccagatcca gccgccgccg ccgcggtgcc agccgccgcc gtggtcgctg tggccgccgc
     1081 ccgagtagcc gtgcccggca ccgccgccac gcgcgccgcg gccttgcgcg tcggcgccgc
     1141 cggcgacgag cgcgagcgac gcgcacgcga ccgccagggc ggtcttcagg gtgaggggga
     1201 agcggtggtt gctcatcgac gatctccttg cagcgctgct gcccatccga tccgcatttt
     1261 gccgcgcagt tcccgccgcg ctggaatcga tatgccgaat tcgatatgcc gaattcttgg
     1321 ttcgcgcgcc gtggcggcga ccgtagtctc gctggccttg cccctggaga cacaagcccc
     1381 atgtccgcga tcctcgacac ctcgccgctg ctgtcgatcc tcgacgccgc ccgcgccaag
     1441 gcgcgcgcca tcaacctgcc gttcgccggc ggcgtgcggc cgcgcgatgc gtacgcgctg
     1501 acccacgccg gcttcgccac gctggtcgac gtgcgcagtg ccgaggagcg ccgcttcgtc
     1561 ggccacgtgc ccgacagcgc gcacgtggcg tgggcgaccg gcaccgcgct gacgcgcaac
     1621 ccgcgcttcg tgcgcgagct cgaagccaag ctgcgcaagg acagcgtcgt gctgctgctg
     1681 tgccgcagcg gcaagcgctc ggcgctcgcg gccgaggcgg cgacgaaggc cggcttcacg
     1741 gccgcgttca acgtgctcga aggcttcgag ggcgagctcg acgcgaacca gcaccgcggc
     1801 ggcgccgacg gttggcgctt ccacgcgctg ccgtggattc aagactgaac ggcggactga
     1861 accatgtcgc tcgacatccc gatcgtcgtc gcgcagctgc gtgaagagcg cagccgctgg
     1921 cgcgcgctgc aaaagcgctg gcgcgatccc ggcgggcgcg agctgccggc gcgcgaggcg
     1981 ctggcgcaga tcgtccaggg cctgcgcggc gcgctgttcc cgatgcgtct cgggccgggc
     2041 gacctgcgcc aggagagcga ggactactac gtcggccaca cgctcgacat cgcgctgaac
     2101 gccttgctcg cgcaggtgcg gctggagctg cgccacgagg cgcggcagca gccgggcttc
     2161 acggccggcg acgccgacga tgcacgcatc gaggcgggct tcgatgcgcg cgccacggag
     2221 ctggtgcgcg agttcgcgct ctcgctcgcg gcgatccgcg cgctgctcga caccgacgtg
     2281 caggccgcgt tccacggcga cccggcggcg cgcagcgtcg acgaggtgct gctgtgctac
     2341 ccgggcatcg aggcaatgat ccaccaccgg ctcgcgcacc ggctgtacgc gctcggcgtg
     2401 ccgctgatcg cgcgcatcgt ggccgagctg gcgcatgccg cgaccggcat cgacatccac
     2461 cccggcgcga ccatcggcgc cggcttcttc atcgaccacg gcaccggcgt cgtgatcggc
     2521 gaaaccgcgg tgatcggcgc gcgggtgcgc gtctaccagg ccgtcacgct gggcgcgaag
     2581 cgcttcccgg ccgacgccca ggggcacctg caaaaaggcc tgccgcgcca cccggtcgtc
     2641 gaggacgacg tggtgatcta cgccggcgcg accgtgctcg gccgcgtcac gctcggcgcc
     2701 ggctcgacga tcggcggcaa cgtctggctc acgcacagcg tgccaccggg cagccatgtc
     2761 acgcaggccg tgccgcaggg cgacggcgcc gacgcccccg cgctgcgcgc ctgaccccac
     2821 gcgcgctgcg cgcgcatctt tcgattcccc ttcccatccg ccacaggagt tcttcatgac
     2881 cgacccagcg cagcaagcgc tcagcgacct cggcgcacgg cagcttgccg atgcgaccaa
     2941 gaccgcgccc aaactcgaca cgatcagccc gcgctggctg acgcacctgc tgcagtggct
     3001 gccggtcgaa gccggcatct accggctcaa caaggtcaag aacccggagg gcgtgctggt
     3061 cgcgtgcacc aaccgcggcg acgagtcgga gctgccgagc accttcgtcg actacgagga
     3121 cacgccgcgc gagtacttcc tgaacggcgt cagcaccgtg ctggacgtgc acacgcgcgt
     3181 cagcgacctc tacaacagcc cgttcgacca gatcaaggag cagctgcgcc tgacgatcga
     3241 gacgatcaag gagaagcagg agagcgagct gatcaacaac cccgactacg gcctgctcgc
     3301 gagcgtggcc gacagccaga tcgtctaccc gctgaccggc gcgcccacgc ccgacgactt
     3361 cgacgagctg ctgaccaagg tctggaagga gccggcgctc ttcctcgcgc acccgctggc
     3421 gatcgccgcg ttcggccgcg agtgcacgcg ccgcggcgtg ccgccgccga ccgtgagcct
     3481 gttcggctcg cagttcatca cctggcgcgg cgtgccgctg attccgtcga acaaggtccc
     3541 ggtcgccgac ggcaagacca aggtcctgct gctgcgcgtc ggcgacaagc gccagggcgt
     3601 ggtcgggctg taccagccgg gcctcgcggg cgagcagagc ccggggctgt cggtgcgctt
     3661 catgggcatc aaccgccacg cgatcgcgtc gtacctggtc tcgctgtact gctcgctcgc
     3721 ggtgcagacg accgacgcgc tggccgtgct cgatgacgtg gaaatcggca agtaccatga
     3781 ctatccagac acctacaagt gacctgagcg ggccgctcga cgcggccgcg ctgaaccagc
     3841 tggcgggcgc gttcttcgcc gcgttgccgg gccaggcgcc gatcggcgcg caggcaccgg
     3901 cgaacccgct tcccgcgggc gcgacgatcg gcccgttcgg caacggcccg ccgctgccgg
     3961 gcacgctggt gccgggcggc aacgcgctgc cgcagtcgcc gcaagacatc gcgtcgatcg
     4021 ccgccgcgca accggcgcgc ttcccgaagg tcgaggccgg caacggcgtg cc
//
LOCUS       DASRDG010000479         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_432725, whole genome shotgun sequence.
ACCESSION   DASRDG010000479 DASRDG010000000
VERSION     DASRDG010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432725"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1147)
                     /locus_tag="VFA35_08040"
     CDS             complement(95..1147)
                     /locus_tag="VFA35_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HZT56160.1"
                     /translation="MLTVHNPQHHLHRGRHEMFRGRLVPCHETTQRLDHVLAELQRRE
                     LGALRPPAVDDAALDAALARTHAPRYLRFLEHAWREWVALDPANAERDALPSVWPAPG
                     MRVDVLPENFAARLGLFAFDAGTPLTAGSWVAARTGAACALDAARAVGGGAVPAAFAL
                     SRPPGHHAGADFFGGYCFLNNTAIAAQALRDGGAQRVAVLDVNYHHGNGTQAIFYARA
                     DVLTVSIHGDPRTEYPFFLGHADERGAGAGKGFNLNLPLPRGTDFTRWRAALRRGLDA
                     VAAFGADALVVALGVDTFAGDPISGFLLRGSDYLQVGRDIAGAKLPTVFAFEGGYAVA
                     EVGVNTVNVLAGFSAV"
     gene            complement(1156..1584)
                     /locus_tag="VFA35_08045"
     CDS             complement(1156..1584)
                     /locus_tag="VFA35_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZT56161.1"
                     /translation="MAFGRLERSHRVSPMSDINMTPLIDVMLVLLVIFMITAPLMTSS
                     LKLDLPKADGAQPNDAPQFISVALDAQGKLFFGDEALEPAAFAARVQDAARKNPRTEV
                     QLRADTGVPYGRVAELIGTVQKAGLNRIGFVTEPAAATPR"
     gene            complement(1607..2266)
                     /locus_tag="VFA35_08050"
     CDS             complement(1607..2266)
                     /locus_tag="VFA35_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZT56162.1"
                     /translation="MTSLATFWSQGDGVIRGVAALLLLMSISAWVVIFWKSWLLHRVR
                     SDIERAVPAFWAAPTLAAGRDALSAFDRERALAPLLGAAVATPPGGTLEAKGHADSQL
                     TRRLRDALHGVLAQLQFGQVLLASIGSTAPFIGLFGTVWGIYHALVGISVAGSMSIER
                     VSGPVGEALVMTAAGLAVAIPAVLAYNVFGKMVASSEAELEGFAHDLREMVLDAGPAS
                     D"
BASE COUNT          328 a          795 c          843 g          303 t
ORIGIN      
        1 cggcgcgtgg gcatcacgct gtcgggcggg aacatcggcc tcgatcgctt cgtggcgctg
       61 gtttcgggcg ccgagtcggt cgactgaaca gcccctacac cgcgctgaag cctgcgagca
      121 cgttgaccgt gttgacgccg acctcggcga ccgcgtagcc gccttcgaag gcgaagaccg
      181 tcggcagttt cgcgccggcg atgtcgcggc cgacctgcag gtagtcgctg ccgcgaagca
      241 ggaaccccga gatcggatcg cccgcgaacg tatcgacgcc gagcgcgacc accagcgcat
      301 cggcgccgaa cgcggccacc gcgtcgagcc cgcggcgcag cgccgcgcgc cagcgcgtga
      361 aatcggtgcc gcgcggcagc ggcaggttca ggttgaagcc ttttcctgcg ccggcgccgc
      421 gctcgtcggc gtggccgagg aagaacgggt actcggtgcg cgggtcaccg tggatcgaca
      481 ccgtgagcac atcggcgcgc gcgtagaaga tcgcctgcgt gccgttgccg tggtggtagt
      541 tgacgtcgag caccgcaacg cgttgcgcgc cgccgtcgcg cagcgcctgc gccgcgatcg
      601 cggtgttgtt caggaagcag tagccgccga agaaatcggc gccggcatgg tggcccggcg
      661 ggcgcgacag cgcgaacgcg gccggcactg cgccaccgcc caccgcgcgc gctgcatcga
      721 gcgcgcaggc cgcgccggtg cgcgcggcaa cccagctgcc ggcggtcagc ggcgtgccgg
      781 catcgaacgc gaacaagccc aggcgcgccg cgaagttctc gggcagcacg tcgacgcgca
      841 tgcccggcgc gggccacacc gagggcagcg cgtcgcgttc ggcgttggcc gggtcgagcg
      901 cgacccattc gcgccacgcg tgctcgagaa accgcaggta gcgcggcgca tgggtgcgcg
      961 cgagcgcggc gtccagtgcc gcgtcgtcga cggccggcgg gcgcagcgcg ccgagctcgc
     1021 ggcgctgcag ttcggcgagc acatggtcga gccgctgcgt ggtctcgtgg cagggcacga
     1081 ggcggccgcg aaacatctcg tggcggcccc ggtgcaggtg gtgctgcggg ttgtggaccg
     1141 tcagcatcgg cgctttcagc gcggcgtcgc ggccgccggt tcggtgacga agccgatccg
     1201 gttcaggccg gccttctgca ccgtgccgat cagctctgcg acgcggccgt agggtacgcc
     1261 ggtgtcggcg cgcagctgca cctcggtgcg cgggttcttg cgcgcggcgt cctgcacgcg
     1321 cgccgcgaac gcggccggct cgagcgcctc gtcgccgaag aacagcttgc cctgggcgtc
     1381 gagcgcgacg ctgatgaact gcggcgcgtc gttcggctgc gcgccatcgg ctttgggcag
     1441 gtcgagcttc aggctcgagg tcatcagcgg cgcggtgatc atgaagatca ccagcagcac
     1501 cagcatcacg tcgatcagcg gcgtcatgtt gatgtcgctc atcggcgaca cgcggtggct
     1561 gcgctcgagg cgaccgaagg ccatggcgtg agcaggccgg ggtcgatcag tcggacgcgg
     1621 ggcctgcgtc gagcaccatc tcgcgcaggt cgtgggcgaa gccttcgagc tcggcctcgc
     1681 tgctcgcgac catcttgccg aagacgttgt aggccagcac cgcggggatc gcgaccgcga
     1741 ggcctgcggc cgtcatcacc agcgcttcgc cgacggggcc cgagacgcgc tcgatcgaca
     1801 tcgagcccgc caccgagatg cccaccagcg cgtggtagat gccccagacc gtgccgaaca
     1861 ggccgatgaa cggcgcggtg ctgccgatcg acgcgagcag cacctggccg aactgcaatt
     1921 gcgcgagcac gccatgcagc gcgtcgcgca ggcggcgcgt gagttgcgag tcggcatggc
     1981 ccttggcttc gagcgtgccg ccgggcggcg tcgcgacggc cgcgccgagc agcggggcga
     2041 gcgcgcgttc gcggtcgaac gcgctcagcg cgtcgcggcc ggcggccagc gtcggcgcgg
     2101 cccagaaggc cggcacggcg cgctcgatgt cgctgcgcac gcggtgcagc agccagctct
     2161 tccagaagat cacgacccag gcgctgatcg acatcagcag cagcagcgcc gcgacgccgc
     2221 ggatcacccc gtcgccctgg ctccagaacg tcgccagact cgtcatgcg
//
LOCUS       DASRDG010000480         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1333038, whole genome shotgun sequence.
ACCESSION   DASRDG010000480 DASRDG010000000
VERSION     DASRDG010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333038"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VFA35_08055"
     CDS             <1..1295
                     /locus_tag="VFA35_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZT56163.1"
                     /translation="NTDGDVAGSPSARLLGTSIAALSNLQHVLVVGSFLRKDHPLLAQ
                     RIRQAARKGAQVHSLHAVHDDWLMPMASTQSVAPSGWLQALADIATALGERNGAQPPL
                     AGRASDDAKAIAASLASGERKAILLGNAAAQHPQAAALLALANWIGAQTGASVGYLTE
                     AANTVGAQLVGALPRSGGLHAGQMLAGPALKACVLLNLEPTLDAGNAAAASAAVQAAE
                     MVVVMTPFKNVANEFADVMLPVAPFTETSGTFVNAEGRVQSFYGVVRPLGETRPAWKV
                     LRVLGNLLALKGFDFETSEEVRAEALGDETTIASRLSNRSSAPAAANAATAAELERIA
                     DVPIYATDSLVRRATSLQLTADARAPFASLPPSLWSQLGLRAGDQVRVSQGAAQALLP
                     AKLDATLAPNCVRVPAGHPDTAALGAMFGAISVEKASA"
     gene            1295..2386
                     /gene="nuoH"
                     /locus_tag="VFA35_08060"
     CDS             1295..2386
                     /gene="nuoH"
                     /locus_tag="VFA35_08060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZT56164.1"
                     /translation="MDWLLAFPQNHPVGWALIWNVAKIVALVLPLMICVAYLTLWERK
                     AIGWSQIRPGPNRVGPLGLLQPIADAVKLIFKEIIVPSAANKRLFFLGPVMTIMPALA
                     AWVVIPFGPEAALSNLNAGLLLLLAITSVEVYGVIIAGWASNSKYAFLGALRASAQMV
                     SYEIAIGFCLVVVLMVAGSLNVTEIVLGQGKGLLAERGLTFLSWNWLPLLPICVVYFI
                     SGLAETNRHPFDVVEGESEIVAGHMVEYSGMAFAMFFLAEYANMILISATSVIMFFGG
                     WYAPVSFGLFGMTTPDWIVQTAWFWNWFWLFAKTFVVVTVFLWVRASFPRYRYDQIMR
                     LGWKIFIPVTLVWLVVVGLWIQSPWNIWN"
     gene            2401..2901
                     /gene="nuoI"
                     /locus_tag="VFA35_08065"
     CDS             2401..2901
                     /gene="nuoI"
                     /locus_tag="VFA35_08065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HZT56165.1"
                     /translation="MTTAASPVKDFFSSFLLGELLKGMALTGRHFWSRKITVQFPEEK
                     TPLSPRFRGLHALRRYDNGEERCIACKLCEAVCPAMAITIESDVRDDGSRRTTRYDID
                     LTKCIFCGFCEESCPVDSIVETHIFEYHGEKRGDLYFTKDMLLAVGDRYEPEIAKAKE
                     ADAKYR"
     gene            2957..3610
                     /locus_tag="VFA35_08070"
     CDS             2957..3610
                     /locus_tag="VFA35_08070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZT56166.1"
                     /translation="MNIEPLLFYIFSAVLLFAAFRVITARSPVYAALYLVLAFFSAAC
                     VWVLLRAEFLAIVLVLVYVGAVMVLFLFVVMMLDLGAEALREGFWTHFPLAAIVGVVI
                     ALEMAAVLMPGTRLTDAPVPTAAALKLGNTKLLGIEVYTNYLYALQIAAVLLLVAIVA
                     AIALTLRKRKDSKYQNPADQVRARPADRMRVVKMQPVVEPPAPAVAASVAPAAGDKP"
     gene            3607..>3759
                     /locus_tag="VFA35_08075"
     CDS             3607..>3759
                     /locus_tag="VFA35_08075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HZT56167.1"
                     /translation="MTITLGHYLTLGAILFALSVVGIFLNRKNLIVLLMAIELMLLAV
                     NMNFIAF"
BASE COUNT          571 a         1286 c         1257 g          645 t
ORIGIN      
        1 cgaacaccga tggcgacgtc gccggctcac cttcggcgcg cttgctgggc acgagcatcg
       61 cggcgctgtc gaacctccaa cacgtgctcg tcgtcggctc gttcctgcgc aaggaccacc
      121 cgctgctcgc gcagcgcatc cgccaggccg cgcgcaaggg cgcgcaggtg cacagcctgc
      181 atgccgtgca cgacgactgg ctgatgccga tggcgtcgac acagtcggtc gcgccgagcg
      241 gctggctgca ggcgctcgcg gacatcgcca ccgcgctcgg cgagcgcaac ggcgcgcagc
      301 cgccgctggc cggccgtgcg agcgacgacg ccaaggcgat cgccgcctcg ctcgcgagcg
      361 gcgagcgcaa ggcgatcctg ctcggcaacg ccgccgcgca gcatccgcag gccgcagcct
      421 tgctggcgct cgcaaactgg atcggcgcgc agaccggcgc cagcgtcggc tacctgaccg
      481 aagccgcgaa cacggtcggc gcgcaactcg tcggcgcgct gccccgaagc ggcgggctgc
      541 acgccggcca gatgctcgcc ggccccgcac tgaaggcctg cgtgctgctg aacctcgagc
      601 ccacgctcga cgcgggcaac gccgccgccg caagtgcggc ggtgcaggcg gccgagatgg
      661 tcgtcgtgat gacgccgttc aagaacgtcg cgaacgagtt cgccgacgtg atgttgccgg
      721 tcgcgccgtt caccgagacc tcgggcacct tcgtgaacgc cgagggccgc gtgcagagct
      781 tctacggcgt cgtccgcccg ctcggcgaga cgcgcccggc ctggaaggtg ctgcgcgtgc
      841 tcggcaacct gctggcgctg aagggcttcg atttcgagac ctcggaagag gtgcgcgccg
      901 aggcgctggg cgacgagacg acgatcgcct cgcggctgag caaccgcagc agcgcgccgg
      961 ccgccgccaa cgccgcgacg gcagccgaac tcgaacgcat cgccgacgtg cccatctatg
     1021 cgaccgattc cctggtgcgc cgcgcaacct cgctgcaact caccgccgac gcgcgcgccc
     1081 cgttcgccag cctgccgcca tcgctgtggt cgcaactcgg actgcgcgcg ggcgaccagg
     1141 tccgtgtgtc gcaaggcgcc gcgcaagcgc tgctgccggc gaaactcgac gcgacgctcg
     1201 cgcctaactg cgtgcgcgtg ccggccgggc accccgacac cgccgcgctc ggcgcgatgt
     1261 tcggcgcgat cagcgtcgag aaagcgagcg cgtgatggac tggctgctcg cgttcccgca
     1321 gaaccacccg gtcggctggg cgctgatctg gaacgtcgcc aagatcgtcg cgctggtgct
     1381 gccgctgatg atctgcgtcg cctatctgac gctgtgggaa cgcaaggcga tcggctggag
     1441 ccagatccgt ccgggcccga accgcgtcgg cccgcttggc ttgctgcagc cgatcgccga
     1501 cgcggtgaag ctgatcttca aggagatcat cgtcccgagc gcggcgaaca agcgcctgtt
     1561 cttcctcggc ccggtgatga cgatcatgcc ggcgctcgcc gcctgggtcg tgatcccgtt
     1621 cgggcccgaa gcggcgctgt cgaacctgaa tgccggcctg ctgctgctgc tggcgatcac
     1681 ctcggtcgag gtctacggcg tgatcatcgc cggctgggcc tcgaactcga agtacgcctt
     1741 cctcggcgcg ctgcgcgcgt cggcgcagat ggtgagctac gagatcgcga tcggcttctg
     1801 cctcgtggtc gttctcatgg tcgcgggcag cctgaacgtg accgagatcg tgctcggcca
     1861 gggcaagggc ctgctggccg agcgcgggct gaccttcctg tcgtggaact ggctgccgct
     1921 gctgccgatc tgcgtcgtct acttcatctc gggcctggcc gagacgaacc ggcatccgtt
     1981 cgacgtcgtc gaaggcgagt ccgagatcgt cgcgggccac atggtcgagt actcgggcat
     2041 ggcgttcgcg atgttcttcc tcgccgagta cgcgaacatg atcctgatct ccgcaacctc
     2101 ggtgatcatg ttcttcggcg gctggtacgc gccggtgtcg ttcggcctgt tcggcatgac
     2161 cacgcccgac tggatcgtcc agaccgcgtg gttctggaac tggttctggc tgttcgccaa
     2221 gaccttcgtc gtcgtcaccg tgttcctgtg ggtgcgggcg agtttcccgc gctatcgcta
     2281 cgaccagatc atgcgcctgg gctggaagat cttcatcccg gtcacgctgg tgtggctggt
     2341 ggtggtgggg ctgtggatcc agtcgccgtg gaacatctgg aattgaaggg ggcgaatcga
     2401 atgaccaccg ccgcatcacc cgtcaaagac tttttctcga gcttcctgct gggcgagttg
     2461 ctcaagggca tggcgctgac cgggcgccac ttctggtcgc gcaagatcac ggtgcagttc
     2521 cccgaagaga agaccccgct gagcccgcgt tttcgcggcc tgcacgcgct gcgccgctat
     2581 gacaacggcg aagaacgctg catcgcgtgc aagctgtgcg aggccgtctg cccggcgatg
     2641 gccatcacga tcgaatccga cgtgcgcgac gacggttcgc gccgcacgac gcgctacgac
     2701 atcgacctga ccaagtgcat cttctgcggc ttctgcgaag agagctgccc ggtggactcg
     2761 atcgtcgaga cccacatctt cgaataccac ggcgagaaac gcggcgacct gtacttcacg
     2821 aaggacatgc tgctggcggt cggcgaccgc tacgaacccg aaatcgccaa agccaaggag
     2881 gcggacgcca agtaccgctg agcctcgacg ctcggcggct tggcgacgcg gcggggagcc
     2941 gcgtgcaaag aacaacatga acatcgaacc cttgctcttc tacatcttct cggcagtgct
     3001 gctgttcgcc gcgtttcgcg tcatcacggc gcgcagcccg gtgtacgcgg cgctgtacct
     3061 ggtgctcgcg tttttcagcg cggcctgcgt ctgggttctg ctgcgcgccg agttcctggc
     3121 gatcgtgctg gtgctggtct acgtcggcgc ggtgatggtg ctgttcctgt tcgtcgtgat
     3181 gatgctcgac ctcggcgccg aggcgctgcg cgagggcttc tggacgcact ttccgctcgc
     3241 cgcgatcgtc ggtgtcgtga tcgcgctcga gatggccgcg gtactgatgc ccggcacgcg
     3301 gctgaccgac gcaccggtgc cgacggcagc cgcgctcaag ctcggcaaca ccaagctgct
     3361 cggcatcgag gtttacacga actacctgta tgcgctgcag atcgcggcgg tgttgctgct
     3421 ggtcgcgatc gtcgcggcga tcgcgctgac gctgcgcaag cgcaaggact cgaagtacca
     3481 aaacccggcg gaccaggtgc gcgcgcgtcc cgccgaccgg atgcgcgtcg tgaagatgca
     3541 gccggtggtc gagccgcctg cgccggcggt cgccgcatcc gtcgcaccgg ccgcaggaga
     3601 caagccatga ccatcaccct cggtcactac ctcacgctcg gcgcgatcct gttcgcgctg
     3661 tcggtggtcg gcatcttcct gaaccgcaag aacctgatcg tgctgctgat ggcgatcgag
     3721 ctgatgctgc tcgccgtcaa catgaacttc atcgcgttc
//
LOCUS       DASRDG010000481         5506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_876564, whole genome shotgun sequence.
ACCESSION   DASRDG010000481 DASRDG010000000
VERSION     DASRDG010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876564"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFA35_08080"
     CDS             <1..192
                     /locus_tag="VFA35_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT56168.1"
                     /translation="KYRYTGVALMTDLGWLIGAAFAPLVALGLSAHFGLAYVSLYLLS
                     GATWTLIALGVNRALEARQ"
     gene            complement(208..927)
                     /gene="fabG"
                     /locus_tag="VFA35_08085"
     CDS             complement(208..927)
                     /gene="fabG"
                     /locus_tag="VFA35_08085"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZT56169.1"
                     /translation="MKRALVTGASGGLGRAIALRLARDGLHVIAHANRRLDAAAALAA
                     EIVQGGGSAEAVAFDVTDRVATHAACAKLLEAGAIQVVVNNAGTHDDAAFPALREAQW
                     HAVMAVSVDGFFNVTQPLTLPMIRTRWGRIINISSVAALGGNRGQVNYAAAKGALNSA
                     TKALSLELASRGVTVNAVAPGIIESGMAQGTFDAAMIERMVPAKRAGRADEVAALVAF
                     LASDDAGYISGQVISINGGML"
     gene            complement(924..1388)
                     /locus_tag="VFA35_08090"
     CDS             complement(924..1388)
                     /locus_tag="VFA35_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HZT56170.1"
                     /translation="MPAPRLTRDGIAALIPHSGTMRLLARLDAWDAQRIACAATNHRD
                     PDHPLRTRRGLLAPAAIEYAAQAMALHGALIGRAAGTPATPGYLASARGVQLHVLRLD
                     DLPAATPDELCIEAVRQAGDARQILYAFTVRHADRLLAEGRAAVVLNTPLAA"
     gene            complement(1391..2200)
                     /locus_tag="VFA35_08095"
     CDS             complement(1391..2200)
                     /locus_tag="VFA35_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HZT56171.1"
                     /translation="MSRLQIWVQGIGLLGPGLPSWAASLDVLRGTLPFASSPTVLPAP
                     LRLPPAERRRAGAAVKLALAVADEAVAQAQLDPALLATVFTSSSGEGANCHALCETLA
                     TPAPMVSPTRFTNSVHNAAAGCWHIAVGSRAASTSLCALDGSFGAGLIEAAASVALGG
                     APLLLVASDSPYPEPLARTRPLPDHFGLALVLTPAPTARSIAALSIGLVGADQAASAC
                     NTCEDAALDALRRAVPAAAALPLLVALARGGAPGAVVLDYLAATRVRVEVS"
     gene            complement(2197..3381)
                     /locus_tag="VFA35_08100"
     CDS             complement(2197..3381)
                     /locus_tag="VFA35_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZT56172.1"
                     /translation="MKPLAITASTLTSALGAGNAATFEALKAERSGLAPVRFMSFPLP
                     TWTGEVEGVDAVAVPPDLGEYDCRNNRLAWLGLQQDGFIDAVAAARARWGASRVAVLL
                     GTSTSGLLQTELAYRRRDADGALPADFHYGPTQNTFSVAAFVAHALRLAGPAWVVSTA
                     CSSSAKVFGNAARLIDAGLIDAAVVGGVDSLCLTTLYGFNSLELLSNEACRPWDAARN
                     GLSIGEAAAFALLERTPAAPPQGWLLGVGESNDGYHMSTPHPEGLGAIAAMRAALADA
                     GLQPADIDYINLHGTATPSNDAAEDAAVDAVFGQATPCSSTKGATGHTLGAAGAVEAA
                     IALLAMRHGLMPAGLNLRQPDPALHLHYLRANRAAPARRVLSNSFGFGGSNASLVLGA
                     AE"
     gene            complement(3378..4112)
                     /locus_tag="VFA35_08105"
     CDS             complement(3378..4112)
                     /locus_tag="VFA35_08105"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZT56173.1"
                     /translation="MNAFAMPAVAWPALLDAASAPYRGAGRFAWHFARGKLGLDPVFR
                     YLLRSGLIPPQARVLDIGCGQGLLASLLQRCAEFERSGRWPSGWAPAPAPARVTGIEL
                     MPRDVSRAQAALGDSAEFICGDMRHTPFPEADAIVILDVLHYIAIAEQNEVLARVRRA
                     LPAGGTLLLRIGDAASTRGFVISQWVDRIVTFVRGHRVVPQFGRTLAAWRAQLESLGF
                     EVRSQPMSAGTPFANVLLVARVKGAA"
     gene            complement(4109..4948)
                     /locus_tag="VFA35_08110"
     CDS             complement(4109..4948)
                     /locus_tag="VFA35_08110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZT56174.1"
                     /translation="MHTAPSSTAADLAPPRWHTPALVKASVACHLGAGALALAQPTLW
                     PWALGAVALDQALITGAGLWPRSTWLGANLLRLPAAAAARREIALTIDDGPDPAVTPA
                     VLDLLDAHRARATFFCIARHAEQHAALCREIVRRGHSVQNHSFDHSHAFALRGPRAIR
                     RELARAQDALAQVTGQRPRFFRAPAGLRNLFLAPVLHELQLQLVAWTRRGFDTARRDP
                     DAVLARLTRGLAAGDILLLHDRHTALAASGRPVTLAVLPALLRRFEQAGLRAVTLPEA
                     LAA"
     gene            complement(4973..>5506)
                     /locus_tag="VFA35_08115"
     CDS             complement(4973..>5506)
                     /locus_tag="VFA35_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZT56175.1"
                     /translation="RSALADLPGARVVAIKPELDALYARYLHEATLQALLGALAVCVL
                     LALRLRSFKRLWRIAQPVAAAVLIVLAGFSLSGAALGILHLVGLLLTVAIGSNYALFF
                     DQIRAQQDAAPPSDPWAVDTDTLASLALANLTAVISFGLLAFSSIPALYAIGQVVAPG
                     ILLSLVLAAAFIPAQRR"
BASE COUNT          738 a         1957 c         2145 g          666 t
ORIGIN      
        1 aaataccgct acaccggcgt cgcgctgatg accgacctgg gctggctgat cggcgccgcg
       61 ttcgcgccgc tggtggcgct gggcctgtcg gcgcactttg gcctcgcgta tgtcagcttg
      121 tacctgttgt ccggagcgac ctggacgctg atcgcgctgg gcgtgaaccg cgcgctcgag
      181 gcccggcaat aggccgcgtg tggcgggtca cagcatcccg ccgttgatcg agatcacctg
      241 cccgctgatg tagcccgcgt cgtcggacgc gaggaacgcg accagtgcgg ccacctcgtc
      301 ggcgcggccg gcgcgtttgg ccggcaccat gcgctcgatc atggccgcat cgaacgtgcc
      361 ctgcgccatg cctgattcga tgatgcccgg cgcgaccgca ttgaccgtga cgccgcggct
      421 cgcgagttcg agcgagagcg ctttcgtcgc gctgttcagc gcgcccttgg cggcggcgta
      481 gttgacctga ccgcggttgc cgcccagcgc ggcgaccgac gagatgttga tgatgcggcc
      541 ccagcgggtg cggatcatcg gcagcgtcag cggctgggtg acgttgaaga agccgtcgac
      601 cgacacggcc atcaccgcgt gccactgcgc ctcgcgcagc gccgggaacg cggcgtcgtc
      661 gtgcgtgccg gcgttgttga cgacgacctg gatcgcgccg gcttcgagca gcttcgcgca
      721 ggccgcgtgc gtggccacgc ggtcggtcac gtcgaacgcg accgcttcgg cgctgccgcc
      781 gccctggacg atctcggcgg cgagcgccgc cgccgcgtcg aggcgccggt tcgcgtgcgc
      841 tatcacatgc aggccgtcgc gcgcgaggcg cagcgcgatc gcgcggccga ggccgccgct
      901 ggcgccggtg acgagcgcgc gtttcatgcg gcgagcggcg tgttcagcac cacggccgcg
      961 cgcccttcgg cgagcagccg gtcggcgtgg cggaccgtga acgcgtacag gatctggcgc
     1021 gcatcgccgg cctggcgcac ggcctcgatg caaagctcgt ccggcgtcgc ggcgggcagg
     1081 tcgtcgagcc gcagcacgtg cagctgcacg ccgcgcgcac tcgccaggta gccgggcgtc
     1141 gccggcgtgc cggcggcccg gccgatcagc gcgccgtgca aggccatcgc ctgtgcggcg
     1201 tactcgatcg cggccggcgc gagcaggccg cgccgcgtgc gcagcgggtg gtcggggtcg
     1261 cggtggttgg tcgcggcgca ggcgatgcgt tgcgcgtccc aggcgtcgag ccgcgcaagc
     1321 aggcgcatcg tgccgctgtg cgggatcagc gccgcgatgc cgtcgcgggt cagccgcggt
     1381 gccggcaggt tcacgatact tcgacccgca cgcgggtggc ggccaggtag tcgagcacga
     1441 cggcgccggg cgcgccgccg cgcgcgagcg cgaccagcaa cggcagcgcc gccgccgccg
     1501 gcacggcacg gcgcagcgcg tcgagcgcgg cgtcctcgca ggtgttgcag gcgctcgcgg
     1561 cctggtccgc gccgacgagc ccaatcgaca gcgcggcgat cgagcgcgcg gtcggggcgg
     1621 gcgtcagcac cagcgccagg ccgaagtgat cgggcagcgg ccgtgtgcgg gcgagcggct
     1681 cggggtaggg cgagtcgctc gcgaccagca gcagcggcgc gccgccgagc gcgacgctgg
     1741 cggccgcctc gatcaggccg gcgccgaagc tgccgtcgag cgcgcacagg ctggtcgacg
     1801 cggcccggct gccgaccgcg atgtgccagc agccggccgc agcgttgtgc accgagttcg
     1861 tgaagcgggt cggcgagacc atcggtgcgg gcgtggcgag cgtctcgcac agcgcgtggc
     1921 agttcgcgcc ttcgccgctg ctcgaggtga acacggtcgc cagcagcgcc gggtcgagct
     1981 gcgcctgcgc gacggcctcg tcggcgaccg cgagcgcgag cttcaccgcg gcgccggcgc
     2041 ggcgccgctc ggccggcggc aggcgcagcg gtgcgggcag cacggtcggc gacgacgcga
     2101 acggcagtgt gccgcgcagc acgtcgagcg acgcggccca cgaggggaga ccgggcccga
     2161 gcaggccgat gccttgaacc cagatctgca accggctcat tccgccgccc ccaacaccag
     2221 gctcgcattc gagccaccga agccgaacga attcgacagc acgcggcgcg ccggcgcggc
     2281 gcggttcgcg cgcaggtagt gcaggtgcag cgcggggtcg ggctggcgca ggttcaggcc
     2341 ggccggcatc agcccgtggc gcatcgccag cagcgcgatc gcggcctcga cggcgccggc
     2401 cgcgccgagc gtgtggccgg tcgcgccctt ggtcgagctg cagggcgtgg cctggccgaa
     2461 cacggcgtcg accgcggcgt cctcggccgc gtcgttgctc ggcgtcgcgg tgccgtgcag
     2521 gttgatgtag tcgatgtcgg ccggctgcag gccggcgtcg gcgagcgcgg cgcgcatcgc
     2581 cgcgatcgcg cccaggccct cggggtgcgg cgtgctcatg tggtagccgt cgttgctctc
     2641 gccgacgccg agcagccagc cctgcggcgg cgcggccggc gtgcgctcga gcagcgcgaa
     2701 cgccgccgcc tcgccgatcg acaggccgtt gcgcgcggcg tcccagggcc ggcaggcctc
     2761 gttcgacagc agctccagcg agttgaaccc gtacagcgtc gtcaggcaca ggctgtcgac
     2821 gccgccgacc acggccgcat cgatcagccc ggcgtcgatc agccgcgcgg cgttgccgaa
     2881 caccttcgcg ctcgacgagc acgcggtcga gacgacccac gccgggccgg ccagccgcag
     2941 cgcgtgggcg acgaaggcgg cgaccgagaa ggtgttctgc gtcgggccgt aatgaaagtc
     3001 ggccggcaat gcgccgtcgg cgtcgcgccg gcggtaggca agctcggtct gcagcagccc
     3061 cgaggtgctg gtgccgagga ggaccgcgac gcggctggcg ccccagcgcg cacgcgccgc
     3121 ggcgacggcg tcgatgaagc cgtcctgctg caggcccagc cacgcgagcc ggttgttgcg
     3181 gcagtcatat tcacccagat cagggggaac tgcgaccgcg tcgacgccct cgacttcgcc
     3241 ggtccaggtc ggcagcggga agctcatgaa gcgcaccggc gccaagccgc tgcgctcggc
     3301 cttcagtgcc tcgaaggtcg cggcgttgcc ggcgccgagc gcgctggtca gcgtgctcgc
     3361 agtgatcgcc agtggcttca tgcggcgccc ttcacgcgcg ccaccagcag cacgttcgcg
     3421 aacggcgtgc ccgcgctcat cggctggctg cgcacctcga aaccgagcga ttcgagctgg
     3481 gcccgccacg ccgccagcgt gcggccgaac tgcggcacga cgcggtggcc gcgcacgaag
     3541 gtcacgatgc ggtcgaccca ctggctgatg acgaagccgc gcgtcgacgc cgcgtcgccg
     3601 atgcgcagca gcagcgtgcc gccggccggc agcgcgcggc gcacgcgcgc cagcacctcg
     3661 ttctgctcgg cgatcgcgat gtagtgcagg acgtcgagga tcacgatcgc gtcggcctcg
     3721 gggaagggcg tgtgccgcat gtcaccgcag atgaactcgg cgctgtcgcc gagcgcggcc
     3781 tgcgcgcggc tcacgtcgcg cggcatcagc tcgatgccgg tcacgcgggc gggcgccggc
     3841 gcgggcgccc agccgctcgg ccagcggccg ctgcgctcga actcggcgca gcgctgcagc
     3901 aggctcgcga gcagcccctg gccgcagccg atgtcgagta cgcgggcctg cggcgggatc
     3961 aggccgcttc gcagcaggta gcggaacacc gggtcgaggc cgagcttgcc gcgcgcgaaa
     4021 tgccaggcga agcgaccggc gccgcggtag ggcgcgctgg cggcgtcgag cagcgcgggc
     4081 caggcgacgg cgggcatcgc gaaggcgttc atgcggccag cgcctccggc agggtcacgg
     4141 cgcgcaggcc cgcctgctcg aagcgccgca gcagcgccgg cagcacggcg agcgtcaccg
     4201 ggcggccgct ggcggcgagc gcggtgtggc ggtcgtgcag cagcaggatg tcgccggcgg
     4261 cgaggccgcg ggtgagacgc gcgagcacgg cgtcggggtc gcggcgtgcc gtgtcgaagc
     4321 cgcgccgcgt ccaggcgacg agttgcagtt ggagttcgtg cagcaccggc gcgagaaaca
     4381 ggttgcgcag cccggccggc gcgcgaaaaa accgcggccg ctggccggtg acctgcgcga
     4441 gcgcatcctg cgcgcgcgcg agttcgcgcc ggatcgcgcg cgggccgcgc agcgcgaagg
     4501 cgtgcgagtg gtcgaagctg tggttctgca cgctgtggcc gcgccggacg atctcgcggc
     4561 acagcgcggc gtgttgttcg gcgtggcgcg cgatgcagaa gaaggtggcg cgggcgcggt
     4621 gcgcgtcgag caggtcgagc accgcgggcg tgacggcggg gtcggggccg tcgtcgatcg
     4681 tcagcgcgat ctcgcggcgc gccgccgccg ccgccggcag gcgcagcagg ttcgcgccca
     4741 gccaggtcga gcgcggccac aggccggcgc cggtgatcag cgcctggtcg agcgcgaccg
     4801 cgcccagcgc ccacggccac agcgttggct gcgcgagggc gagcgcgccg gcgccgagat
     4861 ggcaggcgac gctcgccttc accagcgccg gcgtgtgcca gcgcggcggg gcgaggtcag
     4921 cggcggtcga cgagggcgcg gtgtgcatgg ctggcggatt ttcacacgcc gatcagcggc
     4981 gctgcgccgg gatgaaggcc gccgccaaca ccaggctgag caggatcccc ggcgccacga
     5041 cctggccgat cgcgtacagc gccggaatgc tcgagaacgc gagcaggccg aacgagatca
     5101 ccgcggtcag gttcgccagc gcgagcgagg cgagcgtgtc ggtgtcgacg gcccaaggat
     5161 cggatggcgg cgcggcgtcc tgctgcgcgc ggatctggtc gaagaacagc gcgtagttcg
     5221 agccgatcgc gaccgtcagc agcagcccga ccaggtgcag gatgccgagc gccgcaccgc
     5281 tcaggctgaa ccccgcgagc acgatcagca ccgccgccgc caccggctgg gcgatgcgcc
     5341 acagccgctt gaacgaacgc aggcgcagcg ccagcagcac gcacaccgcc agcgcgccga
     5401 gcagggcctg cagcgtggct tcgtgcaggt agcgcgcata gagcgcgtcg agctcgggct
     5461 tgatcgcgac gacgcgcgcg ccgggcaggt cggccagcgc gctgcg
//
LOCUS       DASRDG010000482         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_780793, whole genome shotgun sequence.
ACCESSION   DASRDG010000482 DASRDG010000000
VERSION     DASRDG010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780793"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFA35_08120"
     CDS             <1..272
                     /locus_tag="VFA35_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56176.1"
                     /translation="ATGRFCDRHRLLLAIAQRLEAALAALARGDAARLTAEFAARLGL
                     VGRRVLVDAGALHDGVLERLDLRALVLDGGRDLPLGLVRSLRAAT"
     gene            complement(287..1069)
                     /locus_tag="VFA35_08125"
     CDS             complement(287..1069)
                     /locus_tag="VFA35_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56177.1"
                     /translation="MTGRPQQGPTLLDRLDLSLAQRITSLGSGAEADLLASAQSYHDR
                     LYRGSSRDPHFLAALLRQRGFAVWDEDAVEVLAGSSKRLPPSTQEHQLILGLMDALQM
                     VRDRSSQGRVPDGWFLVELFRVTTRGLSRFRNNTLRVDDPWDAVLHVAHARPAELTPV
                     LDTFDFARRYRDYAPLFDSLHPLRQGFRVLWRFARIAPFPDFNGMIAWLGMCGWLLAK
                     GYPLLVPEASDRELLKHIVAGPPPTRCVQWEARLLEAVGPLQ"
     gene            1103..>2481
                     /locus_tag="VFA35_08130"
     CDS             1103..>2481
                     /locus_tag="VFA35_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56178.1"
                     /translation="MDAPNAQPAPPPAAPLRPRRRRRFWRALFWTCTSFAMLLAAAPY
                     ALNLTWVRRQIADALTEELGTTVRIDGLGFSWLSGIEARGVEIDNARGFDAAHPCLEL
                     AELRGDLRWPSLLRGRVDFAGTIIGLRVRIDQDEQGRTNFEQLSAHGDASGTRARVRG
                     ADRPHPRSPRPRDDGEFELQQVRIDLAVAGALIEIRRGGRLVESMHDLDCTVRKDFGA
                     EQVTVRLDGKLAALTAGAREGSLHLRADANARTADFALETQGLDLSRYQPLAQALLPG
                     AVTALAGIVNGTCTGKLRFTDPPSIELGGELAVEHPHLAGGWLQGMDLQAERWTLRPA
                     LAVGTGGLPRLDPAFAIDLGFLQLRPLDGARLQQLLAGRPGLGVDCALDLDALVRAGG
                     TPAGHLRGASGRLTGAVALPTPGGALPKPAEFARALVADLRVDGGRVAFGGFEVTQLA
                     GTVALRDGHL"
BASE COUNT          302 a          927 c          925 g          327 t
ORIGIN      
        1 tggcgaccgg gcgcttctgc gaccggcacc gactgctgct cgcgatcgcg cagcggctcg
       61 aggcggcgct cgcggcgctg gcgcgcggcg acgcggcgcg gctcacggcg gagttcgcgg
      121 cgcggctcgg tctcgtcggc cggcgcgtcc tggtcgacgc gggggcgctg cacgacggcg
      181 tgctcgagcg gctcgatctg cgcgcgctcg tgctcgacgg cggccgcgac ctgccgctcg
      241 gcctcgtgcg ttcgctgcgc gccgcgacct gacccggtcg acgcggttac tgcagcgggc
      301 cgacggcttc gagcaggcgc gcttcccact gcacgcagcg cgtcggcggc gggccggcga
      361 cgatgtgctt gagcagttcg cggtcggacg cctcgggcac cagcagcggg tagcccttgg
      421 cgagcagcca gccgcacatg ccgagccacg cgatcatgcc gttgaagtcc gggaacggcg
      481 cgatgcgcgc gaaccgccac agcacgcgga agccctggcg cagcggatgc agcgagtcga
      541 acagcggcgc gtagtcgcgg tagcggcgcg cgaagtcgaa ggtgtcgagc acgggcgtga
      601 gctccgcggg gcgggcgtgc gccacgtgca gcacggcgtc ccacgggtcg tcgacgcgca
      661 gcgtgttgtt gcggaagcgc gacaggccgc gcgtggtcac acggaacagc tcgaccagga
      721 accagccgtc gggcacccgc ccctggctgc tgcggtcgcg caccatctgc agcgcgtcca
      781 tgagcccgag gatcagctgg tgctcctgcg tcgacggcgg cagccgcttg ctgctgcccg
      841 cgagcacttc gaccgcgtcc tcgtcccaga ccgcgaaccc gcgctggcgc agcagcgcgg
      901 cgaggaagtg cgggtcgcgc gagctgccgc ggtagaggcg gtcgtggtag ctctgcgccg
      961 acgcgagcag gtccgcctcg gcgccgctgc cgaggctcgt gatgcgctgc gccaacgaca
     1021 ggtcgaggcg atcgaggagg gtcggcccct gctgcggtcg tccggtcatc ggccctctct
     1081 ttcggcataa tcggcccacc acatggacgc cccgaacgcg cagcccgccc cgccgcccgc
     1141 cgcacccctg cgtccgcgcc ggcggcgccg gttctggcgc gcgctgttct ggacctgcac
     1201 ttcgttcgcg atgctgctcg cggcggcgcc ctacgcactc aacctgacct gggtccggcg
     1261 ccagatcgcc gacgcgttga cggaggagct gggcaccacg gtgcgcatcg acggcctcgg
     1321 tttcagctgg ctctcgggca tcgaggcccg cggtgtcgag atcgacaacg cacgcggctt
     1381 cgacgcggcg catccgtgcc tcgaactcgc cgaactgcgc ggcgacctca ggtggccttc
     1441 gctgttgcgc gggcgtgtgg acttcgcggg cacgatcatc ggactgcgcg tgcgcatcga
     1501 tcaggacgaa cagggcagga ccaacttcga gcagctgtcc gcgcacggcg acgccagcgg
     1561 cacgcgcgcc cgcgtccgcg gcgccgaccg gccgcacccg cggtcgcccc ggccgcggga
     1621 cgacggcgag ttcgaactgc agcaggtgcg catcgacctc gcggtcgcgg gcgcgctgat
     1681 cgagatccgg cgcggcggcc gcctcgtcga gtcgatgcac gacctcgact gcacggtgcg
     1741 caaggacttc ggcgccgagc aggtcacggt ccggctcgac ggcaagctgg cagcgctgac
     1801 cgccggcgcg cgcgaaggct cgctgcacct gcgcgccgac gcgaacgcgc gcaccgccga
     1861 cttcgcgctc gagacgcagg gcctcgacct ttcccgctac cagccgctcg cacaggcgct
     1921 gctgccgggc gccgtcacgg cgctcgccgg catcgtcaac ggcacgtgca cgggcaagct
     1981 gcgattcacc gacccgccgt cgatcgagct cggcggcgag ctcgcggtcg agcatccgca
     2041 cctcgccggc ggctggctgc aggggatgga tctgcaggcc gagcgctgga cgttgcgtcc
     2101 cgcgctcgcg gtcggcaccg gcgggctgcc gcggctcgat cctgcgttcg cgatcgacct
     2161 cggcttcctg cagctgcggc cgctcgacgg cgcgcgcctg cagcagctgc tcgccggccg
     2221 gccggggctc ggcgtcgact gcgccctcga cctcgacgcg ctcgtgcgcg ccggcggcac
     2281 gcctgccgga catctgcgcg gcgccagcgg ccggctcacg ggcgcggtcg cgctgccgac
     2341 gcccggcggc gcgctgccga agccggcgga gttcgcgcga gcgctggtcg ccgacctgcg
     2401 cgtcgacggc ggccgggtcg cgttcggcgg gttcgaggtg acgcagctcg ccggcaccgt
     2461 cgcgctgcgc gacggccacc t
//
LOCUS       DASRDG010000483         4213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_644437, whole genome shotgun sequence.
ACCESSION   DASRDG010000483 DASRDG010000000
VERSION     DASRDG010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644437"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2067
                     /locus_tag="VFA35_08135"
     CDS             <1..2067
                     /locus_tag="VFA35_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZT56179.1"
                     /translation="RGAAYDFISALRVPGTDEVNPCIAYTIDTKRARTELRAQHAQGT
                     LLRELVANASNDANTDPQIGRTLFNLLVPVEVEPFLGGTSEMVIELQPETAGIPWELL
                     DTNPDAQASDRRPWSIRSRLVRKLRVASFRAQVMDANADDEVLVIGEPACDPARYPRL
                     EGARTEAIAVADRLAAPGSGVAPEKVHRLVERNDAQTIINALFERRYRIVHIAGHGAP
                     TGNGGVVLSGKDTYLGANEVRAMRTVPELVFLNCCHLAGRDAATVLANPGLNYDRAGF
                     AATIAEELIKVGVRCVIAAGWAVEDEPAEKFATAFYASLLGGARFIEAVGVARAAAWN
                     ANRQGNTWAAYQCYGDPEWRWQRAGTAAQAAVPSPSDEFAGVASPVALTLALETLAIR
                     SQYSGADPARQLDKLRYLEGEFKSLWGGMGAVAEAFGVAYAGANALDPAIAWLRAALD
                     AEDGSASFRAAEQLGNQLVRRAATLADPAAARHGILEGIAHLERLAALQPTQEREALL
                     GSAYKRLTMIESKADKGRAGKHPAASAALAASVRHYAAAEAMARKSGADNLHYPAKNG
                     IAAELRAAFLDGRVPQLDADRLKAVSDSLNQAAAERPDFWSVVGQSELQMLIALVKGQ
                     LAAIEPALTATLRDLKQRVPAVRMWDSVWNEAQFTLEPYLAIAAPAEQRAARNLLAAL
                     AALAAA"
     gene            complement(2076..2867)
                     /locus_tag="VFA35_08140"
     CDS             complement(2076..2867)
                     /locus_tag="VFA35_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HZT56180.1"
                     /translation="MSVLEAALRREQVVLSLGMAAVVGLAAVYVWDGAGMGMPAWDMT
                     TASLFPHRQRNVPGDMGGSWWLVISMWWVMMIAMMTPGAAPLVLLHGRVLMRAGTPAP
                     RVAASSALLLAGYLLAWLGFSVLAAALQKALQPAGLLSGMMWWSRSAGLSATLLVAAG
                     LYQWSPWKRTCLSQCRNPVMFLTAHGRAGLWGAFVLGVRHGAYCVGCCGMLMALLFVG
                     GVMNVVWIAALTVLVLCEKLMPQGAWIGRATGAVLMVWAAATLWV"
     gene            complement(2864..3508)
                     /locus_tag="VFA35_08145"
     CDS             complement(2864..3508)
                     /locus_tag="VFA35_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HZT56181.1"
                     /translation="MTPWEIQGLELVNCNCSYACPCQFNALPTHGHCEAMGAIRIDKG
                     HYGDVSLDGMKIGFVFRWPGAIHEGKGHCQAAVDERATPAQRDALLKIMSGQDTDPFA
                     TMFSVFASTVETMHQPKFTAIAFDVDVDARRGAISAADLFEITGEPIRNPVTGAEHRV
                     RIDLPDGFEYEIAEIGSASGRSQDPVALNLKNSYGQFARLHLNNHGPIRQRAAA"
     gene            complement(3589..>4213)
                     /locus_tag="VFA35_08150"
     CDS             complement(3589..>4213)
                     /locus_tag="VFA35_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56182.1"
                     /translation="IVLAALLLIGGTLFNTWLDTDPEGLTRALGNAALTGLIAAAVWC
                     TGWALMSKTFTRQAHFGWHVRVFVWASLALLLVGALPALLAFAFSWPVLTDFDFIATI
                     AVVATALYYHLLAVEPAKHRLLRWLALTGALIGVALALWFNLQRSDQLGDELYMSHLF
                     PPALRLAKPVATDAFIGGLATLRPALDRKAKEPARGDEGSRGEDDAP"
BASE COUNT          696 a         1514 c         1453 g          550 t
ORIGIN      
        1 cgcggcgccg cctacgactt catcagcgcg ctgcgcgtgc ccggcaccga cgaggtcaac
       61 ccctgcatcg cctacacgat cgacaccaag cgcgcgcgca ccgagctgcg cgcgcagcac
      121 gcgcagggca cgctgctgcg cgagctggtc gcgaacgcgt cgaacgacgc caacaccgac
      181 ccgcagatcg gccgcacgct gttcaacctg ctggtgccgg tcgaggtcga gcccttcctc
      241 ggcggcacca gcgagatggt gatcgagctg cagcccgaga ccgccggcat cccgtgggag
      301 ctgctcgaca cgaacccgga cgcgcaggcc tcggaccggc ggccgtggtc gatccgcagc
      361 cggctcgtgc gcaagctgcg ggtcgcgagc ttccgcgccc aggtcatgga cgcgaatgcc
      421 gacgacgaag tgctcgtgat cggcgagccc gcctgcgacc cggcgcgcta cccgcgcctc
      481 gaaggcgcgc gcaccgaggc gatcgcggtg gccgaccggc tggccgcgcc gggcagcggc
      541 gtcgcgccag agaaggtgca ccggctggtc gagcgcaacg acgcgcagac catcatcaac
      601 gcgctgttcg agcgccgcta ccgcatcgtc cacatcgccg gccacggcgc gccgaccggc
      661 aacggcggcg tcgtgctgtc gggcaaggac acctacctcg gcgcaaacga ggtgcgcgcg
      721 atgcgcaccg tgcccgagct cgtgttcctg aactgctgcc acctggccgg ccgcgatgcc
      781 gcgaccgtgc tcgcgaaccc gggtctgaac tacgaccgcg ccggcttcgc cgcgacgatc
      841 gccgaggagc tgatcaaggt cggcgtgcgc tgcgtgatcg cggccggctg ggcggtcgag
      901 gacgaaccgg ccgagaagtt cgcgaccgcg ttctacgctt cgctgctcgg cggggcccgc
      961 ttcatcgagg cggtcggcgt ggcgcgcgcc gcggcctgga acgcgaaccg ccagggcaac
     1021 acctgggccg cctaccagtg ctacggcgac cccgaatggc gctggcagcg ggccggcacg
     1081 gccgcgcagg ccgcggtgcc gtcgccgagc gacgagttcg ccggcgtggc ttcgccggtg
     1141 gcgctcacgc tcgcgctcga aacgctcgcg atccgcagcc agtacagtgg cgccgacccg
     1201 gcgcgccagc tcgacaagct gcgctacctc gaaggcgagt tcaagtcgct gtggggcggc
     1261 atgggcgcgg tggccgaggc cttcggcgtg gcctatgccg gcgcgaacgc gctcgacccg
     1321 gcgatcgcct ggctgcgcgc cgcgctcgac gccgaggacg gcagtgcgtc gttccgcgcc
     1381 gccgagcagc tcggcaacca gctggtgcgg cgcgccgcga cgctggccga cccggccgcc
     1441 gcgcgccatg gcatcctcga aggcatcgcg cacctcgaac ggctcgccgc gctgcagccg
     1501 acgcaggagc gcgaggccct gctcggctcg gcctacaagc gcctgacgat gatcgagtcg
     1561 aaggccgaca agggccgcgc cggcaagcac ccggccgcga gcgccgcgct cgcggcctcg
     1621 gtgcggcact acgccgcggc cgaggcgatg gcgcgcaagt cgggtgccga caacctgcac
     1681 tacccggcga agaacggcat cgccgccgaa ctgcgcgcgg cgtttctcga tgggcgcgtg
     1741 ccgcaactcg acgccgaccg gctgaaggcc gtcagcgatt cgctgaacca ggccgcggcc
     1801 gagaggcccg acttctggtc ggtggtcggg cagagcgaac tgcagatgct gatcgcgctc
     1861 gtcaagggcc agctggcggc catcgaaccg gcgctgaccg cgacgctgcg cgacctgaag
     1921 cagcgcgtgc cggcggtgcg catgtgggac tcggtctgga acgaagcgca gttcacgctc
     1981 gaaccctacc tcgcgatcgc ggcgccggcc gagcagcgcg ccgcgcgcaa cctgctcgcg
     2041 gcgctcgccg cgctggccgc ggcctgatcc gtcggtcaga cccacaaggt cgcggccgcc
     2101 cacaccatca gcacagcgcc ggtggcgcga ccgatccatg cgccctgcgg catcagcttc
     2161 tcgcacaaca ccagaaccgt cagcgccgcg atccacacca cgttcatgac cccgccgacg
     2221 aacaggagtg ccatcagcat cccgcagcag cccacgcagt acgcgccgtg gcggaccccc
     2281 aggacgaagg cgccccacaa cccggcacgc ccgtgggcgg tcaggaacat cacgggattg
     2341 cggcactgcg acagacaggt gcgcttccag ggcgaccact ggtaaaggcc ggcggcaacc
     2401 agcagcgtgg ccgacaaccc tgcgctcctc gaccaccaca tcattcctga cagcaggccc
     2461 gccggctgca acgccttctg cagcgcggcc gccagcaccg aaaaacccag ccacgccaac
     2521 aggtaacccg ccaggagcaa ggccgatgac gcggccacac ggggcgccgg tgtgccggcg
     2581 cgcatcagaa cgcggccgtg cagcaagacg agtggcgccg cgcccggcgt catcatcgcg
     2641 atcatcatga cccaccacat cgaaatgacg agccaccatg agccgcccat gtcgcccggc
     2701 acgttgcgct gccggtgtgg aaacaggctc gccgtcgtca tgtcccaagc aggcatgccc
     2761 atgccggcgc cgtcccagac gtagacggcg gcaaggccga ccactgccgc catccccagc
     2821 gagagcacga cttgctcccg ccgcagggcg gcttcgagaa cgctcacgcc gcagcgcgct
     2881 ggcgaatcgg cccgtggttg ttcaggtgca ggcgtgcgaa ttggccgtag ctgttcttca
     2941 ggttgagcgc gacgggatcc tgcgaacgac ccgatgccga tccgatctcc gcgatttcgt
     3001 actcgaagcc gtcgggcagg tcgatgcgca cgcgatgttc cgcgcccgtc acggggttgc
     3061 ggatcggctc gccggtgatc tcgaagagat cggccgccga gatcgcgccg cggcgcgcat
     3121 cgacgtcgac atcgaacgcg atcgccgtga acttgggttg gtgcattgtc tcgacggtcg
     3181 acgcgaacac ggaaaacatc gttgcgaagg ggtcggtgtc ctgacccgac atgatcttga
     3241 gcaaggcgtc gcgctgcgca ggcgtcgcgc gctcgtcgac ggccgcctgg cagtggccct
     3301 tgccttcatg gatcgccccg ggccagcgga agacgaatcc gatcttcatg ccgtcgagcg
     3361 acacgtcgcc atagtggccc ttgtcgatgc gaatggcgcc catcgcttcg cagtggccgt
     3421 gggtcggcag cgcgttgaac tgacacgggc aagcgtagga gcagttgcag ttgacaagct
     3481 ccaagccttg gatttcccag ggggtcatgt gagcctccgt cacaaacagt tgtcgagcct
     3541 cccgaaatta gtcctcgggt gatcggctgt caatgaagcg cacgtcggtc acggtgcgtc
     3601 gtcttcgccg cggctgcctt cgtcgccgcg cgccggctcc ttggccttcc ggtcgagcgc
     3661 cgggcgcagc gtggccaggc cgccgatgaa ggcgtcggtc gcgaccggtt tcgccaggcg
     3721 cagcgcgggc gggaacaggt ggctcatgta gagctcgtcg ccgagctggt cgctgcgctg
     3781 caggttgaac cacagcgcca gcgcaacgcc gatgagcgcg cctgttagcg cgagccagcg
     3841 cagcagccgg tgcttggccg gctcgaccgc gagcaggtgg tagtacagcg cggtcgcgac
     3901 caccgcgatc gtcgcgatga agtcgaagtc ggtgagcacc ggccacgaga acgcgaacgc
     3961 gagcaaggcg ggcagcgcgc cgacgagcag cagtgccagg ctcgcccaga cgaacacgcg
     4021 cacatgccag ccgaagtgcg cctggcgcgt gaacgtcttc gacatcagcg cccagccggt
     4081 gcaccagacc gccgcggcaa tcagtccggt gagcgcagca ttgccgagcg cgcgggtcag
     4141 gccctcgggg tcggtgtcga gccaggtgtt gaacagcgtg ccgccgatca gcagcagcgc
     4201 ggcgagcacg atc
//
LOCUS       DASRDG010000484         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_780933, whole genome shotgun sequence.
ACCESSION   DASRDG010000484 DASRDG010000000
VERSION     DASRDG010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780933"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..466
                     /locus_tag="VFA35_08155"
     CDS             5..466
                     /locus_tag="VFA35_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZT56183.1"
                     /translation="MTTLTVNGKKLSVDADPASPLLWVLREDLQLTGTKFGCGMAQCG
                     ACTVHVNGQPTRSCVTPVSSVQGKRITTIEGLSVSADHPLQKAWIAEQVPQCGYCQSG
                     QIMTAAALLEKNRKPTREQIVEHMKGNLCRCGTYNHIVAAIQRAAGEMQKA"
     gene            475..>2645
                     /locus_tag="VFA35_08160"
     CDS             475..>2645
                     /locus_tag="VFA35_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZT56184.1"
                     /translation="MNFEFRHLSRRAFLLSTGAAGVAIGFGSLLNVRKAFGQAAPFSP
                     NGWVRVGTDNVVTVYAPACEMGQGVYTAMPLLIAEEMDLDWIRVRIEQAPYNPKVFGN
                     PLFGGAMLAGASRTTRGYYTIMRLAGMQGRAIMVDNAAKKWGVPAAECSTEPSAVLHK
                     ASGRRMSYGEIAAFAEVPAIPPNFTPEQLKPMSRFRLIGKDVPRVEIPDKVSGQARYG
                     IDVRMPGMMYAAVLRAPVNGERPLQVNDADAKKVAGVKHIVPLPYGVAVVADTYPAAQ
                     KGKQALKVEWSQASKARSYTTDKVIPEYMQRVRNLDDKGTVYEAHGDAQGAIAKAAKR
                     ISAEYTSLNVTHATMEPQNCTARVDGDAIEFWAPTQSPFGVFLAAVKGAGFKPENVKI
                     NVTFLGGGFGRRAENDYALDAALIAKAVPGYPIKTIWSREDDTRYTKPRPLTVQRLEA
                     GLDAQGNLVGLRTRICAESIYRRFAPPAFQASGGKDLPLCEGAYEPTYHYPNFSLEQL
                     LEARGVDVAVWRSVGGGYTKFALETFIEELAQAAGKDPIEFRMKLLEKDPRGQAVIRE
                     AMALADWNRKRPAGRALGLAYSDIWETYVAMVAEVSVDRKSGNVNVHELWSAVDTGVA
                     LQPANVRAQIESAAVWGVSALRERLLYKDGVPQQSNFHDYPVLRMNEAPKITTKVIVT
                     DNKPGGIGECGLPPVAPAVANAVFKLTGKRLRDLPFKQELL"
BASE COUNT          481 a          890 c          902 g          372 t
ORIGIN      
        1 cgacatgacg acattgaccg tgaacggcaa gaagctgagc gtggatgccg acccggccag
       61 ccccctgctg tgggtgctgc gagaagacct gcaactcacc ggcaccaagt tcggctgcgg
      121 catggcgcag tgcggcgcct gcacggttca cgtgaacggc cagcccacgc gctcgtgcgt
      181 cacgcccgtc tcttctgttc aaggaaagcg gattacgacg atagaagggc tgtcggtcag
      241 cgccgatcat ccgttgcaga aagcctggat cgccgagcag gtgccgcagt gcggctactg
      301 ccagtccggc cagatcatga ccgccgcggc gctgctcgag aagaaccgca agcccacgcg
      361 cgagcagatc gtcgagcaca tgaagggcaa cctctgccgt tgcggcacct acaaccacat
      421 cgtcgccgcg atccagcgcg cggcgggcga aatgcagaaa gcgtaggagg cgcgatgaac
      481 ttcgaattca ggcatctttc gcgccgagca ttcctgctgt cgaccggcgc tgccggcgtg
      541 gcgatcggtt ttggaagtct gctgaacgtc cgcaaggcgt tcgggcaggc agcgcctttc
      601 tcgcccaacg gctgggtgcg cgtcggaacc gacaacgtgg tgaccgtgta cgcgcccgcg
      661 tgcgaaatgg ggcagggtgt gtacaccgcg atgccgctcc tcatcgccga ggagatggac
      721 ctcgactgga tccgggtgcg catcgagcag gcgccttaca acccgaaggt gttcggcaac
      781 ccgctcttcg gcggcgccat gctcgccggc gcatcgcgca ccacgcgcgg ctactacacg
      841 atcatgcggc tcgcgggcat gcagggccgc gcgatcatgg tcgacaacgc ggcgaagaag
      901 tggggcgtgc cggccgccga gtgctcgacc gagccgagcg ccgtgctgca caaggcctcc
      961 ggccggcgca tgagctacgg ggagatcgcg gctttcgccg aggtgccggc aatcccgccg
     1021 aacttcacgc ccgaacagtt gaagccgatg agccggttcc gcctcatcgg caaggacgtc
     1081 ccgcgggtcg agatcccgga caaggtctcg gggcaggcgc gctacggcat cgacgtgcgc
     1141 atgccgggca tgatgtacgc ggcggtcctg cgcgcgccgg tgaacggcga gcgcccgctg
     1201 caggtgaacg acgccgacgc gaagaaggtc gcgggcgtga agcacatcgt gccgctgccg
     1261 tacggcgtcg ccgtggtcgc cgacacctac ccggccgcgc agaagggcaa gcaggcgctc
     1321 aaggtcgaat ggagccaggc atcgaaggcg cgctcgtaca cgaccgacaa ggtcatcccg
     1381 gaatacatgc agcgggtgcg caacctcgac gacaagggca ccgtctacga ggcccacggc
     1441 gatgcgcagg gcgcgatcgc caaggcggcg aagcgcattt cggccgagta cacctcgctt
     1501 aacgtgaccc acgcgaccat ggagccgcag aactgcaccg cccgcgtcga cggcgacgcg
     1561 atcgagttct gggcgcccac gcagtcgccc ttcggcgtgt tcctcgcggc cgtgaaaggc
     1621 gccggcttca agcccgagaa cgtcaagatc aacgtgacct tcctcggcgg gggcttcggc
     1681 cggcgcgccg agaacgacta cgcgctcgac gcggcgctga tcgccaaggc ggtgccgggc
     1741 tatccgatca agaccatctg gagccgagag gacgacaccc gctacacgaa accccggccg
     1801 ctcaccgtgc agcggctcga ggctggactc gacgcgcagg gcaacctcgt cggcctgcgt
     1861 acgcgcatct gcgccgagtc gatctaccgt cgcttcgcgc cgcccgcgtt ccaggcgagc
     1921 ggcggcaagg acctgccgct gtgcgaaggc gcctacgagc ccacctatca ctatcccaac
     1981 ttctcgctcg agcagttgct cgaagcgcgc ggcgtcgacg tcgcggtgtg gcgctcggtc
     2041 ggcggcggct acaccaagtt cgcgctcgag accttcatcg aggagttggc gcaggcggcg
     2101 ggcaaggacc cgatcgaatt ccgcatgaag ctgctggaga aggacccgcg cggccaggcg
     2161 gtgatccgcg aggcgatggc gctcgccgac tggaaccgca agcgtcccgc gggccgcgcg
     2221 ctgggcctcg cctacagcga catctgggaa acctacgtcg cgatggtcgc ggaggtctcg
     2281 gtcgaccgca agagcggcaa cgtcaacgtg cacgagctct ggagcgcggt cgataccggc
     2341 gtcgcgctgc agccggcgaa cgtgcgcgcg cagatcgagt cggcggcggt ctggggcgtg
     2401 agcgccctgc gcgagcgcct cctctacaag gacggggtgc cccagcagtc gaacttccac
     2461 gactacccgg tgctgcgcat gaacgaggcg ccgaagatca cgaccaaggt catcgtcacc
     2521 gacaacaagc ccggcggaat cggcgagtgc ggcctgccgc cggtcgcgcc ggccgtggcg
     2581 aacgcggtct tcaagctcac cggcaagcgc ttgcgcgatc ttccgttcaa gcaggaactg
     2641 ctgaa
//
LOCUS       DASRDG010000485         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_74006, whole genome shotgun sequence.
ACCESSION   DASRDG010000485 DASRDG010000000
VERSION     DASRDG010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74006"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="VFA35_08165"
     CDS             <1..1591
                     /locus_tag="VFA35_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT56185.1"
                     /translation="NRFLLSDLFASPSPEKLGRDARVVDVLGPAVRSAERAFAGQPRV
                     AAEVLDAVGVSYRAIGLIPEAEQVTRRALALLGDDRGDPLTLRARGHLVAVLSGLLRT
                     AEALELGAAVVDESKRRLGADDAETLARRVDLAGARWDAGQPEQSIADLRAVVADQAR
                     VLGAEDARTLATSAQLGVNLAANGAIDEGRKLLTETLAMEDRVLGADHPETLTTRSHL
                     AWIHTLGSDWQQADAVYGQILPTMTALYGKDHPYTARTTVQMAQSWMHLGRLDDAEAV
                     VRAACADLERRLGKDHAWTQDAHLMLEEALFRKRDFAAAIKEGEQIVEQRRAAGGENL
                     AHALIKLGRSHQDAGEHRAAADAFREAAQIYRVAFAKPTDFLAQALYNLGVVTRDGGD
                     AQGAIAPLEEAMAIDTELWGADHSHVLGDRYNLARTLLAAGKPAEAVEQMRLVMASPA
                     RGKSEPWRQLCHEAVLGQALVEGRQDQEGERCLVEAITGLDGLGRGGDAPARQARASL
                     VQMYERLGRTQDAERWRSGNH"
     gene            complement(1640..>2095)
                     /locus_tag="VFA35_08170"
     CDS             complement(1640..>2095)
                     /locus_tag="VFA35_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56186.1"
                     /translation="QVDLAGVVANGTRELEFAGGRVRVEIADEDLLAAPQTVEVLPGE
                     TKTLRFPIAIGRTRELRFNGDGRQHPEYRTPLHVVVRGAGGAVIEQQDIEALMPDLRG
                     FRYWYLTRAYPFGRYEVEAHGETGLCYRGSFEVREDPEDPTRVDVPLAK"
BASE COUNT          293 a          755 c          792 g          255 t
ORIGIN      
        1 caaccggttc ctgctgtcgg acctgttcgc ctcgccgtcg cccgagaagc tcggccgcga
       61 cgcgcgcgtc gtcgacgtgc tggggccggc cgtgcgctcg gccgagcggg cgttcgcggg
      121 ccagccgcgc gtcgcggccg aggtgctgga cgcggtcggc gtcagctacc gcgcgatcgg
      181 gctgatcccg gaggccgaac aggtgacgcg ccgggcgctc gcgctgctcg gcgacgaccg
      241 cggcgatccg ctgacgctgc gcgcgcgcgg ccacctggtc gcagtgttgt ccggcctgct
      301 gcgcaccgcc gaagcgctcg aactgggtgc cgccgtcgtc gacgagagca agcgccgcct
      361 cggcgccgac gatgcggaga cgctcgcgcg gcgcgtcgac ctcgcgggag cgcgctggga
      421 cgcgggccag cccgagcaga gcatcgccga cctgcgcgcc gtggtcgcgg accaggcccg
      481 cgtgctcggc gccgaggacg cgcgcacgct cgccacgagc gcccagctcg gcgtgaacct
      541 ggccgccaac ggcgcgatcg acgagggccg caagctgctg accgagacgc tggcgatgga
      601 ggaccgcgtg ctgggggccg accatcccga aacgctgacc acgcgcagcc acctggcctg
      661 gatccacacg ctgggcagcg actggcagca ggccgatgcg gtctacggcc agatcctgcc
      721 gacgatgacg gcgctgtacg gcaaggacca cccgtacacc gcgcggacca ccgtgcagat
      781 ggcgcagtca tggatgcacc tcggccgcct cgacgacgcg gaggcggtcg tccgcgccgc
      841 ctgcgccgac ctcgagcggc gcctcggcaa ggatcacgcc tggacccagg acgcccacct
      901 gatgctcgaa gaggccctgt tccgcaagcg cgacttcgcc gcggcgatca aggaaggcga
      961 acagatcgtc gagcagcggc gcgccgcggg cggcgagaac ctcgcgcacg cgctgatcaa
     1021 gctcggccgc agccaccagg atgccggcga gcaccgcgcg gcggcggacg cgttccgcga
     1081 ggcggcgcag atctaccgcg tggcgttcgc gaagccgacg gacttcctcg cgcaggcgct
     1141 ctacaacctc ggcgtggtga cgcgcgacgg cggcgacgca cagggcgcga tcgcgccgct
     1201 ggaggaggcg atggcgatcg acaccgagct gtggggcgcg gatcactccc acgtgctcgg
     1261 cgaccgctac aaccttgcgc gcacgctgct cgccgcgggc aagccggccg aggccgtcga
     1321 gcagatgcgc ctggtgatgg catcgccggc gcgcggcaag agcgagccct ggcgccagct
     1381 ctgccacgaa gcggtgctcg gccaggcgct cgtcgagggc cggcaggatc aggaagggga
     1441 acgctgtctg gtcgaggcga tcaccggcct cgacgggctc ggccgcggcg gcgacgcgcc
     1501 ggcgcggcag gcgcgcgcga gcctcgtgca gatgtacgag cggctggggc gcacgcagga
     1561 cgccgagcgc tggcgcagcg gcaatcacta gcctggctgt tcttcatcgg tcggtccctc
     1621 cgctgcgggt ccgcgcgagc tacttggcga gcggcacgtc gacgcgcgtc gggtcctccg
     1681 ggtcctcgcg cacctcgaag ctgccgcggt agcagaggcc cgtttcgccg tgtgcctcga
     1741 cctcgtagcg gccgaacggg taggcgcggg tgaggtacca gtagcggaag ccgcgcaggt
     1801 cgggcatcag cgcttcgatg tcctgctgct cgatgacggc gccgccggcc ccgcgcacga
     1861 ccacgtgcag cggcgtgcgg tactcggggt gctgccggcc gtcgccgttg aagcgcagct
     1921 cgcgcgtgcg gccgatcgcg atcgggaaac gcagcgtctt cgtctcgccg ggcagcacct
     1981 cgacggtctg cggcgcggcg agcagatcct cgtcggcgat ctcgacgcgg acgcggccgc
     2041 cggcgaactc gagctcgcgg gtgccgttcg cgacgacgcc ggcgaggtcg acctg
//
LOCUS       DASRDG010000486         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_998376, whole genome shotgun sequence.
ACCESSION   DASRDG010000486 DASRDG010000000
VERSION     DASRDG010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998376"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2882
                     /locus_tag="VFA35_08175"
     CDS             <1..>2882
                     /locus_tag="VFA35_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZT56187.1"
                     /translation="LASPSFSAALLLSAAVFVLLLAPWRGWPAFGLAALLDELRARRR
                     PPGLAETINNSVELAHRLTAREDLYFPQGLIVSLALFALTLGAFAIAEIGVELPETTR
                     LIALCVYALVLAPLAHWLIVRASLAAILDQRDLLRPSHHHDARPQRTPPSLEAAITSL
                     PSVSVAVPVSVATPAPVPAPAPIDQGELDARLLRHARGCDGAAALAALAQGADPNLVP
                     DAGDRDQRSVVVLACVAQDLALLRALIAKGADINRAHAGLTPLIAATRDSYQGRPDVV
                     VTLLTNGADPQCADTEGNTPLHFAVRAAEPTVAALLCDASAPIDAVNREGYTPLAIAC
                     VLGRGDLVRFLIERRADVHVERAVPALIAAAASPDDNTDVVKLLLKRGARIDVSDGHG
                     RAPLAIAAQHDNADIVALLLKSGAAIDAADALGVTALMEAALAGAADALDVLGANAPM
                     LEAADHGGRTALMFAAESMYADDAFVAHLLALGASSATTTAAGKRAVDFAAAAGRWSV
                     VKLLDPDYALPASVDEGATTAAVAREDSPAHLLDALRFGHWQIADGFAETQRAWPMAR
                     RAQLFLDLAEHRDPAARAWLLCHGLDANATLPNGTTLVGALLAQLPTSLAALREAFDA
                     GAQMSGVGVLGSVFDAIARKPDARSGLETLALAMIERGADIFAADANAQTPLARSIAC
                     GSARIADALLRRGVDPNLRDRHGRTPLFAAFALPAEAAEAVTRALVRAGADPELASAN
                     GETALGLALASAQTSPRRWLDWTQWKLPKRALRATDLPAAAQLGDADAVAKLIDLGFA
                     VDAPDAQGASALLRAAGAGHTDIVMLLLERGADLAQAAASGATPLSAAVTARRAGVVD
                     VLLDRGMPVDQRLPGGSTVLMIAAALGYPDIVANLLARGADANAQDERGKRPLHAAAQ
                     FAFVGRDTTRARQTLELLIGKGAELDARDAKGSTALHTLLGAHAAP"
BASE COUNT          378 a         1131 c          964 g          409 t
ORIGIN      
        1 tcctcgccag cccatcgttc agcgccgcgc tgctgctctc cgccgcggtg ttcgtgctgc
       61 tgctcgcgcc gtggcgcggc tggcccgcgt tcggcctggc cgcgctgctg gatgaactgc
      121 gcgcgcggcg acgcccgcca ggtctcgccg aaacaatcaa caacagcgtc gagctggcgc
      181 atcgcctgac cgcgcgcgaa gacctgtatt tcccgcaagg cctcatcgtc tcactcgccc
      241 tgttcgcgct gacgctcggc gccttcgcga tcgcggagat cggcgtcgag ctgcccgaaa
      301 cgacgcgcct gatcgcgctc tgcgtctatg cgctggtgct cgcgccgctc gcccactggc
      361 tgatcgtgcg cgcgagcctc gccgctatac tcgatcagcg cgacctgctg cgcccatcgc
      421 atcatcacga tgcacggccg caacgcacgc cgccgtcgct ggaggccgcg atcacgtcgc
      481 tgccgtccgt ttcggttgca gtgcccgtat ccgtcgccac tcccgcgccg gtccccgctc
      541 ccgcaccgat cgaccagggc gaactcgatg cgcgcctgct gcgtcacgcg cgcggctgcg
      601 acggcgcggc ggcactcgcc gcgctcgcgc aaggcgccga tcccaacctc gtgcccgacg
      661 cgggtgaccg cgaccagcgc tcggtcgtcg tgctcgcctg cgtcgcccag gatctcgccc
      721 tgctgcgcgc gctgatcgcc aagggcgccg acatcaaccg cgcgcacgcc gggctgactc
      781 cgctgatcgc ggcgacgcgc gacagctacc agggccggcc cgacgtggtc gtcactttgc
      841 tcaccaacgg cgccgatccg cagtgcgccg acaccgaggg caacacgccg ctgcacttcg
      901 ccgtgcgcgc ggccgagccg accgtcgccg cgctgctgtg cgatgcctcc gcgccgatcg
      961 acgcggtcaa ccgcgagggc tatacgccgc tcgcgatcgc ctgcgtgctc ggccgcggcg
     1021 acctcgtgcg cttcctcatc gaacgccgcg ccgatgtcca cgtcgagcgc gccgtgcccg
     1081 cgttgatcgc cgccgcggcc tcgcccgacg acaacaccga tgtggtcaag ctgctgctca
     1141 agcgcggcgc gcgcatcgac gtcagcgacg gccacggtcg cgcgccgctt gcgatcgccg
     1201 cgcagcacga caacgccgac atcgtcgcgc tgctgctcaa gtccggggcc gcgatcgatg
     1261 cggccgatgc gctcggcgtc accgcgctca tggaagccgc gctcgccggc gccgccgacg
     1321 cgctcgacgt gctcggtgcg aatgcgccca tgctcgaggc cgccgaccac ggcggccgca
     1381 ctgcgctgat gttcgctgcc gaatcgatgt acgccgacga cgccttcgtc gcgcacctgc
     1441 tcgcgctcgg cgcctcgtcc gccacgacga cggccgcggg caagcgcgcg gtcgatttcg
     1501 ccgccgcggc cggacgctgg agcgtggtca agctgctcga cccggattac gcgttgccgg
     1561 ccagcgtcga tgagggcgca acgactgccg ccgtggcgcg cgaggactcg cccgcgcacc
     1621 tgctcgacgc gctgcgcttc ggccactggc agatcgcgga cggcttcgcc gaaacgcagc
     1681 gcgcctggcc gatggcgcgg cgcgcccaac tttttctcga cctcgccgag catcgcgatc
     1741 cggcggcgcg cgcctggctg ctgtgccatg gcctcgacgc caacgcgacg ctgcccaacg
     1801 gcaccacgct cgtgggcgcc ctgctcgcac aactgccgac gagcttggcc gcgctgcgcg
     1861 aggcgttcga tgccggcgcg cagatgtccg gcgtcggcgt gcttggctcg gtattcgatg
     1921 cgatcgcacg caaacccgat gcgcgcagcg gactcgaaac gctggcattg gcaatgatcg
     1981 agcgcggcgc ggatattttc gccgccgatg cgaatgcaca gactccgctc gcgcgcagca
     2041 tcgcctgcgg cagcgcgcgc atcgccgacg cgctgctccg gcgcggagtc gatccgaacc
     2101 tgcgcgatcg gcacggccgc acgccgctgt tcgcggcctt cgccctgcct gccgaggccg
     2161 ccgaagcggt cacgcgcgcg ctggtgcgcg cgggcgccga tccggaactc gcgtcggcca
     2221 acggcgaaac cgcgctcggc ctcgccctcg cctccgcaca gacgagcccg cgccgctggc
     2281 tcgactggac gcaatggaag ctgcccaagc gcgcgctgcg tgcgaccgac ttgccggccg
     2341 ccgcgcagct cggcgatgcg gatgcagtgg cgaaactgat cgacctcggc tttgccgtcg
     2401 atgcgcccga cgcgcaaggc gcgagcgcgc tgctgcgcgc cgcgggcgcg ggccacaccg
     2461 acatcgtcat gctcctgctc gagcgcggcg ccgacctcgc ccaggccgcg gcgagcggtg
     2521 cgacgccgct gtccgccgcg gtcaccgcac gccgcgccgg cgtggtcgat gtgctgctcg
     2581 atcgcggcat gccggtcgac cagcgcctgc ccggcggcag cactgtgctg atgatcgccg
     2641 cggcattggg ttatccggat atcgtggcaa accttctcgc gcgcggtgcc gatgccaacg
     2701 cgcaggacga acgcggcaag cgcccgctgc acgccgccgc ccagttcgcc ttcgtcggcc
     2761 gcgacaccac gcgcgcacgg cagacgctgg aactgctgat cggcaagggc gccgaactcg
     2821 atgcgcgcga cgccaagggc agcaccgcgc tgcacacctt gctcggcgca cacgcggcac
     2881 cg
//
LOCUS       DASRDG010000487         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_433858, whole genome shotgun sequence.
ACCESSION   DASRDG010000487 DASRDG010000000
VERSION     DASRDG010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433858"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFA35_08180"
     CDS             complement(<1..734)
                     /locus_tag="VFA35_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZT56188.1"
                     /translation="MSAPLIFITGASSGIGQALAARFHRAGYRLALVARRAVELQAWA
                     QAQGFDVASFAVYAADVRDVNAIVAAGRACIAAQGVPDVVIANAGVSIGMDTAEFDDL
                     EVMRATFETNNLGMAATFHPFIVPMTARGSGTFVGIASVAAIRGLPGHGAYCASKAGV
                     VGYCESLRGELRAGGVKVVTIVPGYIDTPLTRGNPYSMPFLMPADAFADQAFEAIAGG
                     VSYRVIPWQMGVVAKLMRALPNALYD"
     gene            complement(772..1422)
                     /gene="lptC"
                     /locus_tag="VFA35_08185"
     CDS             complement(772..1422)
                     /gene="lptC"
                     /locus_tag="VFA35_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZT56189.1"
                     /translation="MSATSAAPRAPWQNPAPWYLRAIDTASTYLPLLLMALLALGTWW
                     LVKNTPLFEGGRAAAPPRHEPDYTMTQFMVQRFGSNGAMRVQIEGDLMRHYPDTDTFE
                     IDQPRIRAIGDDGRITTATARLALSNRDGSEVQLSGGAHVVREATPAQAAIDFRGEFL
                     HYFQFTERVRSHLPVVVTQGGSEIRADAMAYDNLARVLDLTGHVRAVLSPPSAATP"
     gene            complement(1433..1999)
                     /locus_tag="VFA35_08190"
     CDS             complement(1433..1999)
                     /locus_tag="VFA35_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZT56190.1"
                     /translation="MLEPVLRHPPELLLQAQGIRWAIFDVDGVLTDGRIYIGAQGEEF
                     KAFSTLDGHGLKLLANHGITPVVITGRDSPAVRRRVADLGIAHAVYGAGDKLVAANAL
                     MPQLGLGWDTLCAMGDDWPDLPLLTRAAFACAPANAHIEVKAVAHHVTAVHGGHGAAR
                     ECCDLLLMAAGRYANALHGHLATLDRAG"
     gene            complement(1999..>2122)
                     /locus_tag="VFA35_08195"
     CDS             complement(1999..>2122)
                     /locus_tag="VFA35_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZT56191.1"
                     /translation="AVDAADLMEQHRITGVLVVDANGRLAGALNSNDLMRAKVI"
BASE COUNT          301 a          748 c          767 g          306 t
ORIGIN      
        1 ttgtcgtaca gcgcgttcgg cagcgcccgc atgagcttcg cgaccacgcc catctgccac
       61 gggatcacgc ggtagctcac gccgccggca atcgcctcga acgcctggtc ggcgaacgcg
      121 tcggccggca tcaggaacgg catgctgtac gggttgccgc gggtcagcgg cgtgtcgatg
      181 tagcccggga cgatcgtcac gaccttgacg ccgccggcgc gcagctcgcc gcgcaggctc
      241 tcgcaatagc cgaccacccc ggccttgctc gcgcaatacg cgccgtgccc cggcaggccg
      301 cggatcgcgg cgacgctcgc gatgcccacg aacgtgccgc tgccgcgcgc cgtcatcggc
      361 acgatgaacg gatggaaggt ggcggccatc ccgaggttgt tggtctcgaa ggtcgcgcgc
      421 atcacctcga ggtcatcgaa ctcggccgtg tccatgccga tgctgacgcc ggcgttcgcg
      481 atcacgacgt cgggcacgcc ctgcgccgcg atgcaggcgc ggccggcggc gacgatcgcg
      541 ttcacgtcgc gcacatcggc cgcatagacc gcgaaactgg ccacgtcgaa gccctgcgcc
      601 tgcgcccacg cctgcagctc gaccgcgcgc cgcgcgacca gcgcgagccg gtagccggcg
      661 cggtggaagc gcgcggccag cgcctggccg ataccgctcg acgcgccggt gatgaatatc
      721 aacggcgcac tcatgaggcg gcgccggtca cgaacggcag cggcgcgctc gtcatggcgt
      781 cgctgcgctc ggcggcgaga gcaccgcgcg cacatggccc gtcaggtcga gcacgcgggc
      841 gaggttgtcg taggccatcg cgtcggcgcg gatctcgctg ccgccttgcg tcacgaccac
      901 cggcaggtgc gagcgcacgc gttcggtgaa ctggaagtag tgcaggaact cgccgcgaaa
      961 gtcgatcgcg gcctgcgccg gcgtggcctc gcgcaccacg tgcgcgccgc cgctgagctg
     1021 cacctcgctg ccgtcgcggt tcgacagcgc caggcgcgcg gtcgcggtgg tgatgcggcc
     1081 gtcgtcgccg atcgcgcgga tgcgcggctg gtcgatctcg aaggtgtcgg tgtcggggta
     1141 gtggcgcatc aggtcgccct cgatctgcac gcgcatcgcg ccgttgctgc cgaagcgctg
     1201 caccatgaac tgggtcatcg tgtagtcggg ctcgtggcgc ggcggcgcgg cggcgcggcc
     1261 gccctcgaac agcggcgtgt tcttcaccag ccaccaggtg ccgagcgcga gcagcgccat
     1321 cagcagcagc ggcagatacg tcgaggcggt gtcgatcgcg cgcaggtacc agggcgcagg
     1381 gttctgccag ggtgcgcgcg gcgcggcgct cgtcgcgctc aagccttgtt cctcatccgg
     1441 cgcggtccag cgtggcgagg tgcccgtgca gcgcgttggc gtagcggccg gcggccatca
     1501 gcagcaggtc gcagcactcg cgcgcggcgc catggccgcc gtgcacggcg gtgacatggt
     1561 gcgcgaccgc cttgacctcg atgtgcgcgt tcgccggcgc gcaggcgaac gcggcgcggg
     1621 tcagcagcgg caggtcgggc cagtcgtcgc ccatcgcgca cagcgtgtcc cagcccaggc
     1681 cgagctgcgg catcagcgcg ttcgcggcga cgagcttgtc gccggcgccg tagaccgcat
     1741 gcgcgatgcc cagatcggcc acgcgccggc gcaccgcggg cgagtcgcgg ccggtgatca
     1801 cgaccggtgt gatgccgtgg ttcgcgagca gtttcaggcc gtggccgtcg agcgtcgaga
     1861 aggccttgaa ctcctcgccc tgcgcgccga tgtagatgcg gccgtcggtc agcacgccgt
     1921 cgacatcgaa gatcgcccag cggatgcctt gcgcctggag cagcagctcc ggtggatggc
     1981 gcagaacggg ctccagcatc agatgacctt tgcgcgcatc agatcgttcg agttcagcgc
     2041 gcctgccagc cggccgttcg catcgacgac gagcacaccg gtaatgcgat gctgctccat
     2101 cagatcggcg gcgtcgaccg cg
//
LOCUS       DASRDG010000488         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1679177, whole genome shotgun sequence.
ACCESSION   DASRDG010000488 DASRDG010000000
VERSION     DASRDG010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679177"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..690)
                     /locus_tag="VFA35_08200"
     CDS             complement(46..690)
                     /locus_tag="VFA35_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HZT56192.1"
                     /translation="MKFTAYERTLQGTGASRRLRNAQKVPGIVYGAGKASMIELDHNA
                     LFFALKKEAFHSSILEMELAGKSEQVLLRDYQMHPFRPIVLHVDFQRVDATTRIVKKV
                     PLHFINEENSPAVKTDASIVNHVATELRISCLASQLPESLTIDLGELTKGQTLHVNDV
                     KLPAGIKVMTQGKPNPVIVSVVAQAAEEEIVAPVVTAAPAEKTKAKKTEKQNKK"
     gene            complement(798..1760)
                     /locus_tag="VFA35_08205"
     CDS             complement(798..1760)
                     /locus_tag="VFA35_08205"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZT56193.1"
                     /translation="MLFNTLLFTGNANPALAQEIATHLGVELGKARVGRFSDGEVDCE
                     IQQNVRARDIFVIQPTCAPTNENLMELCIMVDALRRASARRVTAVIPYYGYARQDRRP
                     RSKRVPISAKVVANMLEAVGVERLLTMDLHADQVQGFFNIPVDNIYASPVLLSDLQSK
                     RYDDLVVVSPDVGGVVRARALAKQMNCDLAIIDKRRPKANVSEVMHVIGEIEGRTCVI
                     MDDMTDTAGTLVKAADVLKERGAKHVYAYCTHAVLSGPALERIKNSRLDEVVITNTIP
                     QSDAVKNCPKLRQLSVAFLFAETIRRISDGESVTSLFAEQNANF"
     gene            complement(1864..1940)
                     /locus_tag="VFA35_08210"
     tRNA            complement(1864..1940)
                     /locus_tag="VFA35_08210"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1904..1906),aa:Gln,seq:ttg)
     gene            complement(1980..>2464)
                     /gene="ipk"
                     /locus_tag="VFA35_08215"
     CDS             complement(1980..>2464)
                     /gene="ipk"
                     /locus_tag="VFA35_08215"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348498.1"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZT56194.1"
                     /translation="GADVPFFVGGRNAIVEGIGERLTPAPVPEQWLAVVKPPTSIETR
                     AIFASPLLVRDTEAAILESFAVNPKQGVMETLLQGGFGTNDLQAAAKAECAQVGQALL
                     LLQRQFGNGRMTGSGSAVFARAGTGPEPSAAMPAIAPGWTGRMCRSLSVHPLVGWAAD
                     "
BASE COUNT          404 a          804 c          786 g          470 t
ORIGIN      
        1 ggctccagca tcacccttgc gggtgcgctc cttcggcctg tcggcctact tcttgttctg
       61 tttttcggtc ttcttggcct tggtcttctc ggccggcgcg gcggtcacga ccggggcgac
      121 gatctcttct tcggcggctt gcgccacgac cgagacgatc accgggttcg gcttgccctg
      181 cgtcatgacc ttgatgccgg ccggcagttt gacgtcgttg acgtgcaggg tctggccctt
      241 ggtcagctcg cccaggtcga tcgtcaggga ctcgggcagc tgcgaagcca ggcacgagat
      301 gcgcagctcg gtggccacgt gattgacgat cgaggcgtcg gtcttgacgg ccggcgagtt
      361 ctcttcgttg atgaagtgca gcggcacctt cttgacgatc cgggtcgtcg catcgacgcg
      421 ctggaagtcg acgtgcagca cgatcgggcg gaacgggtgc atctggtagt cgcgcagcag
      481 aacctgctcg ctcttgccgg ccagttccat ttcgaggatc gacgagtgga acgcttcctt
      541 cttcagtgcg aagaacaggg cgttgtgatc gagttcgatc atcgatgcct tgccggcacc
      601 gtagacgatc ccggggacct tttgcgcatt gcgcaggcgg cggctcgctc cggtcccctg
      661 caacgtgcgc tcgtaagcgg tgaatttcat ctctagctcc aaaatgtgaa ggcccgcgac
      721 caggcgcttc gtcaagacgc gagaccgact agcctcgcga cagggggcgc gtcgccgaag
      781 cgctgcgccc ccgtgttcta aaagttggcg ttctgctcgg cgaacagcga cgtgaccgat
      841 tcgccgtcgg aaatgcggcg gatcgtctcg gcgaacagaa aggccaccga cagctggcgc
      901 agcttcgggc agttcttcac cgcgtcgctc tgcggaatcg tgttcgtgat caccacctcg
      961 tcgaggcgag aattcttgat gcgctcgagc gccgggccgg agagcaccgc gtgcgtgcaa
     1021 taggcgtaga cgtgcttcgc gccgcgctcc ttcagcacgt cggccgcctt gaccagcgtg
     1081 ccggccgtat cggtcatgtc gtccatgatc acgcaggtgc ggccctcgat ctcgccgatc
     1141 acgtgcatca cttccgagac gttggccttc ggccggcgct tgtcgatgat cgccagatcg
     1201 cagttcatct gcttggccag cgcgcgcgcg cgcaccacgc cgcccacgtc gggcgagacg
     1261 accaccaggt cgtcgtagcg cttgctctgc agatcggaca gcagcaccgg cgatgcgtag
     1321 atgttgtcga ccgggatgtt gaagaagccc tggacctggt cggcgtgcag gtccatcgtc
     1381 agcagccgct cgacgccgac ggcctcgagc atgttggcga cgaccttcgc gctgatcggc
     1441 acgcgcttcg agcgcgggcg gcgatcctgg cgcgcatagc cgtagtacgg gatcacggcg
     1501 gtgacgcggc gcgccgaagc gcggcgcagt gcatcgacca tgatgcacag ttccatcagg
     1561 ttctcgttgg tcggcgcgca ggtcggctgg atcacgaaga tgtcgcgggc gcgcacgttc
     1621 tgctggatct cgcagtcgac ctcgccgtcg gagaagcgcc cgacgcgggc cttgccgagc
     1681 tcgacgccca ggtgcgtggc gatctcctgc gccagcgcag ggttcgcatt cccggtgaac
     1741 agcaaggtgt tgaacagcac gttattcatc ccgggggtca cgcatacccg gcgcgccggg
     1801 accaaaattc tcccggcgcg gccgggagca agacactccc ggccgcgccg ggatcaagat
     1861 atttggcagg ggaagaagga ttcgaacctt cgcatgtcgg aatcaaaatc cgatgcctta
     1921 accaacttgg cgactcccct acacaggtcg acagcctttc gactgccaac cgcaactttt
     1981 cagtctgcgg cccacccgac cagcgggtgg acgctcaggc tgcggcacat cctgccggtc
     2041 caacccggtg caatggccgg catggctgcc gagggttccg gcccggtgcc tgctctcgca
     2101 aacaccgcac tgcccgagcc cgtcatgcgg ccgttgccga actgccgctg caacaacaac
     2161 aacgcctgac ccacttgcgc gcattctgcc ttcgccgcgg cctgcaggtc gttggtgccg
     2221 aaaccgcctt gcaagagggt ttccatcacg ccctgcttgg ggtttacagc gaagctttca
     2281 agtatagcag cttcggtatc tctcaccagc agcgggctgg caaaaatggc acgggtctcg
     2341 atgcttgtcg gcggtttcac gacagccagc cactgctcgg gcaccggtgc cggggtcagc
     2401 cgctcgccga tgccttcgac gatcgcgttg cgcccgccga cgaagaacgg cacgtcggcg
     2461 ccca
//
LOCUS       DASRDG010000489         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_74727, whole genome shotgun sequence.
ACCESSION   DASRDG010000489 DASRDG010000000
VERSION     DASRDG010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74727"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VFA35_08220"
     CDS             <1..1293
                     /locus_tag="VFA35_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZT56195.1"
                     /translation="VRFEGFKGSIDDAVNEGVRRGYNHPDNTLRASVVADPQFERKNT
                     KDNTPAVIHMTLVPGNTVDVQIAAKGGGSENKSKFVMMNPSDSLVDWVMKTVPTMGAG
                     WCPPGMLGIGIGGTAERAMLLAKRSLMEDIDMYELKARGPQNKVEELRIELCDKINAL
                     GIGAQGLGGLTTVLDVKIATYPTHAASKPVAMIPNCAATRHAHFVLDGSGPAYLEPPS
                     LDLWPDVKWQPDYNKSKRVNLDTLTKEQVAAWQPGDTLLLSGKMLTGRDAAHKRIQDM
                     LAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTEMMLARTGLIGMI
                     GKAERGPAGIEAIRKHKSAYLMAVGGAAYLVSKAIKQARVVGFADLGMEAIYEFDVTD
                     MPVTVAVDSNGVSVHETGPAAWAAKIKSGIGGVPVAVA"
     gene            1301..2464
                     /locus_tag="VFA35_08225"
     CDS             1301..2464
                     /locus_tag="VFA35_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HZT56196.1"
                     /translation="MTRPAPQPFFRDRNSHTKVTNEELFFDLIYAFAITQLSHRLLEH
                     LTLVGALQTLVLWFAVWLGWQYTAWVTNWFNPENRGMRFMLFGVMLLGLLMSAAIPDA
                     FGANGLMFAACYAAIQIGRSAFVLVRLRGDTALRPNFTRIFGWLCISGVLWIAGGLSD
                     GDARLGFWIAAVLCEYISPMTGFRLPGLGRSNTSDWTIDGAHLAERCQLFVMIALGES
                     ILATGGSFSQSAHLDAPTLIALAVAFAGSVAMWWIYFDTASEHGTHAITRSADPGRIG
                     ARFHYIHVTLIAGIIVSAVADELVVHHPAHHVDLAAACVLVGGPLIYLLGNAAYKKVV
                     YGWFPPSHIAGAALLAILFPVSFATDRLMVGGLASLILIGVAAWQTLRAPSAA"
     gene            complement(2480..>2677)
                     /locus_tag="VFA35_08230"
     CDS             complement(2480..>2677)
                     /locus_tag="VFA35_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZT56197.1"
                     /translation="FANGGTIGFKCSSPEQVRQFHDVAVAHGGTSVEDPPGLREGKLG
                     ALHLSYVRDPDGNKLCALHRP"
BASE COUNT          441 a          916 c          898 g          422 t
ORIGIN      
        1 gtgcgcttcg aaggcttcaa gggcagcatc gacgacgccg tgaacgaagg cgtgcgccgc
       61 ggctacaacc accccgacaa cacgctgcgc gcgtcggtgg ttgccgatcc gcagttcgag
      121 cgcaagaaca ccaaggacaa cacgcccgcg gtgatccaca tgacgctggt gcctggcaac
      181 acggtcgacg tgcaaatcgc ggccaagggc ggcggcagcg agaacaagag caagttcgtg
      241 atgatgaacc cgagcgattc gctggtcgac tgggtcatga agacggtgcc gacgatgggc
      301 gccggctggt gcccgccggg catgctgggc atcggcatcg gcggcaccgc cgagcgcgcg
      361 atgctgctgg ccaagcgcag cctgatggaa gacatcgaca tgtacgagct gaaggcccgc
      421 ggcccgcaga acaaggtcga ggagctgcgc atcgagctgt gcgacaagat caacgcgctg
      481 ggcatcggcg cgcaaggcct gggcggcctg accaccgtgc tcgacgtcaa gatcgcgacc
      541 tacccgacgc acgccgcgag caagccggtc gcgatgatcc cgaactgcgc cgcgacgcgc
      601 cacgcgcact tcgtgctcga cggctccggc ccggcttacc tggagccgcc gagcctggac
      661 ctgtggcccg acgtgaagtg gcagccggac tacaacaagt cgaagcgcgt caacctcgac
      721 accctgacca aggagcaggt cgccgcctgg cagcccggcg acacgctgct gctcagcggc
      781 aagatgctga ccggccgcga cgccgcgcac aagcgcatcc aggacatgct cgcgaagggc
      841 gagaagctgc cggtggactt cacgaaccgg gtgatctact acgtcggccc ggtcgacccg
      901 gtgcgcgacg aggtcgtcgg cccggccggc ccgaccaccg cgacgcggat ggacaagttc
      961 accgagatga tgctggcgcg gaccggcctg atcggcatga tcggcaaggc cgagcgcggc
     1021 cccgccggca tcgaggcgat caggaagcac aagagcgcgt acctgatggc ggtgggcggc
     1081 gcggcgtatc tcgtgtcgaa ggcgatcaag caggcacgcg tcgtcggctt tgccgacctg
     1141 ggcatggaag cgatctacga gttcgacgtg accgacatgc cggtcaccgt cgcggtcgat
     1201 tccaacggtg tttcggtgca cgagaccggc ccggcggcct gggccgcaaa gatcaaatcg
     1261 ggcatcggcg gcgtgcccgt ggcggtggcc tgatgtcggc atgacccgcc ctgcccccca
     1321 acccttcttc cgcgaccgca acagccacac caaggtcacc aacgaagaac tgttcttcga
     1381 cctgatctac gcgttcgcga tcacccagct ctcgcaccgc ctgctcgagc acctgacgct
     1441 ggtcggcgcg ctgcagacgc tggtgctgtg gttcgcggtc tggctcggat ggcaatacac
     1501 cgcgtgggtc accaactggt tcaaccccga gaaccgcggc atgcgcttca tgctgttcgg
     1561 ggtgatgctg ctgggcttgt tgatgtcggc ggcgatcccc gatgcgttcg gcgcgaacgg
     1621 gctgatgttc gcggcctgct acgcggcgat ccagatcggt cgcagtgcct tcgtgctcgt
     1681 gcgcctgcgc ggcgacaccg cgctgcggcc gaacttcacg cgcatcttcg gctggctgtg
     1741 catctcgggc gtgctctgga tcgccggcgg cctgagcgac ggcgacgctc gcctgggctt
     1801 ctggatcgcc gcagtgctgt gcgagtacat ctcgccgatg accggcttcc ggctgcccgg
     1861 cctcggccga tccaatacct ccgactggac catcgacggc gcgcacctcg ccgagcgctg
     1921 ccagctgttc gtgatgatcg cgctgggcga gtcgatcctc gccaccggcg ggtcgttcag
     1981 ccagtcggcg cacctcgacg cgccgacgtt gatcgcgctg gccgtggcct tcgccggcag
     2041 cgtcgcgatg tggtggatct acttcgacac cgcgagcgag cacggcacgc acgcgatcac
     2101 gcgctcggcc gacccgggtc ggatcggcgc ccgcttccac tacatccatg tcacgctgat
     2161 cgccggcatc atcgtctcgg ccgtggccga cgagctggtc gtgcaccacc ctgcccacca
     2221 cgtcgacctc gcggccgcct gcgtgctggt cggcggcccg ctgatctacc tgctcggcaa
     2281 cgcggcctac aagaaggtgg tgtacggctg gttcccgccg tcgcacatcg cgggcgcggc
     2341 gctgctggcg atcctgttcc ccgtctcgtt cgcgaccgac cggttgatgg tcggcgggct
     2401 ggccagcctg atcctgatcg gcgtcgcagc ctggcaaacg ctgcgcgcgc cgagtgcggc
     2461 gtgagccgcg cttgtcacgt cagggccggt ggagcgcaca gagcttgttg ccgtccggat
     2521 cgcggacata cgacaagtgc aatgcaccga gcttgccctc acgcaagccc ggagggtctt
     2581 cgaccgatgt tccaccgtgc gcaacggcga catcgtggaa ctgccgcact tgctctggcg
     2641 aactgcactt gaagccgatg gtgccgccgt tcgcgaa
//
LOCUS       DASRDG010000490         3452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1334431, whole genome shotgun sequence.
ACCESSION   DASRDG010000490 DASRDG010000000
VERSION     DASRDG010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334431"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFA35_08235"
     CDS             <1..621
                     /locus_tag="VFA35_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZT56198.1"
                     /translation="AGVPVLLRSAERLRRVVPAGADGRLVVLVAPADRAHHLPPLLPA
                     LTSLGAEVADGGDCRQQSMQRGLDAAGPCDLVLVHDAARPLFPIDAARRCIERAAAVG
                     AALLALPATDTLKRAGPDGLVQGTLDRRSIWCAQTPQVVRRELLVRALQRAAGESFDA
                     TDDVGLVEAIGGRVALVEGSPRNVKITRPDDLAIAEALLGLERDSP"
     gene            618..1115
                     /gene="ispF"
                     /locus_tag="VFA35_08240"
     CDS             618..1115
                     /gene="ispF"
                     /locus_tag="VFA35_08240"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZT56199.1"
                     /translation="MNDELRIGLGIDLHRLAPGRPCVLAGVRIDSDLGPQAVSDGDVV
                     LHAAVDAVLGAAGLDDLGTLFPENDPQNRGRDSAEFCRRALLLVREQGLRVVSLDVVV
                     ETEVPRLRPHRPAMRRRLAALFDVAADRVNVKGKSGEGLDAIGSRLAIRATVVALLAP
                     LQRGA"
     gene            complement(1128..3062)
                     /locus_tag="VFA35_08245"
     CDS             complement(1128..3062)
                     /locus_tag="VFA35_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT56200.1"
                     /translation="MTAAEAPTTDLRTLLRRLWAAILAHKPLVAAAVVFGVLEALFTK
                     APFVLIDPLLRVMAEQTGAKTLLGRIADWLHHFDKGLADRAAGIEAKESWVDWFARWF
                     QQQSDGIAGWFGRDAQSAGGPAMNLVIGAALVAAASGLLGAFCIYGVQLTSRFFAIKM
                     VADLRCAVARHILSLPLRYFGQRRMGELISNVTNDAQVLQRAFELASDNVVVDPMMIL
                     GNVAVIVFVMPQALLLLVVMIPLMAVPMYRTGRKVRKRSSRTLAAMGDATESMNQILS
                     GIRTVKAFRLEQPRLQEYEQQNATFLQRTMKMLRAKSMSMTQTFVGYQLGFAFLVLLL
                     GYLVLVTGDVKFPSVATVLLPLSTTYQHVKRLTRSYNTLVESTSALDRIEHILNEQPD
                     VADSGGEPLPELRGAVELDDVWFAYGDEPVLRGVSLVVKPGQTVAFVGPSGAGKSTTM
                     DLLMRFHDPQRGRVLIDGKDLRTLRMDDYRRHTAVVSQQPFLFNSSIRENIAYGRPGC
                     TQAEIEAAAKAANIHDFVVALPQGYDTLAGERGCNLSGGQMQRITIARAIVRDPAILF
                     LDEATASLDSENEGLVQRALDNLRRGRTSFVIAHRLSTIAQADLIVVFEQGRIVETGT
                     HEQLVALGGTYKRMRDLQTA"
     gene            complement(3104..>3452)
                     /locus_tag="VFA35_08250"
     CDS             complement(3104..>3452)
                     /locus_tag="VFA35_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56201.1"
                     /translation="LAKFGEFFANDEKKGLLGNIPLNQIKQQLRESEFTELLDGYSIA
                     SFLFYDAEVSKTYGPIQGPDGWFIARINSRTPAQRKLDVKVPRERELVTEDYLNHRFT
                     EWANEVISRVRVE"
BASE COUNT          476 a         1237 c         1229 g          510 t
ORIGIN      
        1 gccggcgtgc ccgtcctgct gcgcagcgcc gaacgcctgc ggcgggtcgt gcccgccggc
       61 gccgacggcc gcctggtcgt gctcgtggcg cccgccgacc gcgcacatca cctgccgccg
      121 ctgctgcccg cgctgacctc gctcggcgcc gaggtcgccg acggcggcga ttgccgccag
      181 cagtcgatgc agcgcggcct cgacgcggcg gggccgtgcg acctggtgct ggtgcacgac
      241 gccgcgcggc cgttgttccc gatcgacgcc gcgcgccgct gcatcgaacg cgcggccgcg
      301 gtcggcgcgg cgctgctcgc gctgcccgcc accgacacgc tgaagcgcgc cggcccggac
      361 ggcctcgtgc agggcacgct cgaccggcgc tccatctggt gcgcgcagac gccgcaggtg
      421 gtgcgccgcg agttgctggt ccgcgcgctg cagcgcgccg ccggcgagag cttcgacgcc
      481 accgacgacg tcggcctcgt cgaagcgatc ggcggccgcg tggcgctggt cgagggcagc
      541 ccgcgcaacg tcaagatcac gcggcccgac gacctcgcga tcgccgaggc gctgctcggg
      601 ctcgaacggg attcgccatg aacgacgagc tgcgcatcgg cctcggcatc gacctgcacc
      661 gcctcgcgcc gggtcgcccg tgcgtcctcg ccggcgtccg catcgacagc gacctcggcc
      721 cgcaggcggt gagcgacggc gacgtcgtgc tgcacgcggc ggtcgacgcc gtgctcggcg
      781 ccgctggact cgacgacctc ggcacgctgt tccccgagaa cgacccccag aaccgcgggc
      841 gcgactcggc ggagttctgc cggcgcgcgc tgctgctcgt gcgcgagcag ggcctgcgcg
      901 tcgtctcgct cgacgtggtg gtcgagaccg aggtgccgcg gctgcggccg caccggccgg
      961 cgatgcgccg ccggctcgcg gcgctgttcg acgtcgcggc cgaccgcgtg aacgtcaagg
     1021 gcaagagcgg cgaggggctg gacgcgatcg gcagccgtct ggcgatccgc gccacggtgg
     1081 tggcgctgct ggcgccgctg cagcgcggcg cctgacgcgg ccgcgcgcta cgccgtctgc
     1141 aggtcgcgca tccgcttgta ggtgccgccg agcgccacca gttgctcgtg cgtgccggtc
     1201 tcgacgatgc ggccctgctc gaacacgacg atcaggtcgg cctgcgcgat cgtcgacagg
     1261 cggtgcgcga tcacgaacga ggtgcggccg cggcgcaggt tgtcgagcgc gcgctgcacg
     1321 aggccttcgt tctccgagtc gaggctcgcg gtcgcctcgt cgaggaacag gatcgccggg
     1381 tcgcgcacga tcgcgcgcgc gatcgtgatg cgctgcatct ggccgcccga caggttgcag
     1441 ccgcgctcgc ccgcgagcgt gtcgtagccc tgcggcagcg cgacgacgaa gtcgtggatg
     1501 ttggcggcct tcgccgccgc ctcgatctcc gcctgcgtgc agcccggccg gccgtaggcg
     1561 atgttctcgc ggatcgagct gttgaacagg aacggctgct ggctcacgac cgcggtgtgc
     1621 cggcggtagt cgtccatgcg cagcgtgcgc aggtccttgc cgtcgatcag cacgcggccc
     1681 cgctgcgggt cgtggaagcg catcagcaga tccatcgtcg tcgacttgcc ggcgccggac
     1741 gggccgacga acgcgacggt ctggccgggc ttcaccacga ggctgacgcc gcgcagcacc
     1801 ggctcgtcgc cgtaggcgaa ccagacgtcg tcgagctcga cggcgccgcg cagttcgggc
     1861 aggggctcgc cgccggaatc ggcgacgtcg ggctgctcgt tcaggatgtg ctcgatgcga
     1921 tcgagcgcgc tggtcgactc gaccagcgtg ttgtaggagc gcgtcagccg cttcacgtgc
     1981 tggtaggtcg tcgacagcgg cagcagcacg gtcgcgaccg aggggaactt cacgtcgccg
     2041 gtgaccagca ccaggtagcc gagcagcagc accaggaacg cgaacccgag ctggtagccg
     2101 acgaacgtct gcgtcatcga catcgacttc gcgcgcagca tcttcatcgt gcgctgcagg
     2161 aacgtcgcgt tctgctgctc gtactcctgc agccgcggct gctcgagccg gaatgccttg
     2221 acggtgcgga tgcccgacag gatctggttc atcgactcgg tcgcgtcccc catcgcggcc
     2281 agcgtacggc tgctgcgctt gcgcaccttg cggccggtcc ggtacatcgg caccgccatc
     2341 agcgggatca tcacgaccag cagcagcagc gcctgcggca tgacgaacac gatcacggcg
     2401 acgttgccga ggatcatcat cgggtcgacg accacgttgt cgctggcgag ctcgaacgcg
     2461 cgctgcagca cctgcgcgtc gttggtgacg ttgctgatca gctcgcccat gcggcgctgg
     2521 ccgaagtagc gcagcggcag ggacaggatg tgccgcgcca ccgcgcagcg caggtcggcg
     2581 accatcttga tcgcgaagaa gcgcgacgtc agctgcacgc cgtagatgca gaacgcgccg
     2641 agcaggccgg aagcggccgc gaccagcgcg gcgccgatca ccaggttcat cgccggcccg
     2701 ccggccgatt gcgcgtcgcg gccgaaccag cccgcgatcc catcggactg ctgctggaac
     2761 cagcgcgcga accagtcgac ccaggactcc ttcgcctcga tgccggcggc ccggtcggcg
     2821 aggcccttgt cgaagtggtg cagccagtcg gcgatccttc cgagcaaggt ctttgcgccg
     2881 gtctgctcgg ccatgacccg gagcagcggg tcgatcagca cgaacggcgc cttggtgaac
     2941 agcgcctcga gcacgccgaa gacgactgcg gccgcgacca gcggcttgtg cgccaggatc
     3001 gcggcccaga ggcggcgcag cagcgtccgc aggtcggtgg tcggggcttc cgcggcggtc
     3061 acgccgcgat cctatgcgaa gctgcccctg ttccgcccgg cccctattcc acccgcacgc
     3121 gcgaaatcac ctcgttcgcc cactcggtga agcggtggtt caggtagtcc tcggtgacca
     3181 gctcgcgctc gcgcggcacc ttgacgtcga gcttccgctg cgccggcgtg cgcgagttga
     3241 tgcgcgcgat gaaccagccg tccgggccct ggatcgggcc gtaggtcttg ctgacctcgg
     3301 cgtcgtagaa caggaacgac gcgatcgagt agccgtcgag aagctcggtg aactcgctct
     3361 cccgcagctg ctgcttgatc tggttcagcg ggatgttgcc cagcagaccc ttcttctcgt
     3421 cgttggcgaa gaactcgccg aacttggcca gc
//
LOCUS       DASRDG010000491         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_528659, whole genome shotgun sequence.
ACCESSION   DASRDG010000491 DASRDG010000000
VERSION     DASRDG010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528659"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..603
                     /locus_tag="VFA35_08255"
     CDS             106..603
                     /locus_tag="VFA35_08255"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZT56202.1"
                     /translation="MANPPTSIYDFDAVSIEGRPAQLSTQRGKVMLIVNTASACGFTP
                     QFAGLEALWKTYADKGLVVIGFPSNEFGGQDPGSDGEIASFCQLNYGVSFPMMSKVKV
                     NGAQAHPLWQWLTKEAPGFLGSTSVKWNFTKFLVGRDGQVLKRYAPTDKPESIKRDIE
                     AALAA"
     gene            608..1807
                     /locus_tag="VFA35_08260"
     CDS             608..1807
                     /locus_tag="VFA35_08260"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HZT56203.1"
                     /translation="MFEHIPPFAGDPILSLNEDFQKDPRPNKINLSIGIYFDDAGRLP
                     VMDAVRRAEAIVVAKGGPKPYLPIEGAANFRAAVQQLLFGAGHEAITSGRVATIQSVG
                     SSGGLKVGADFLARYFPGSQVWVSDPTWDNHRSMFEGAGLTVNAYPYYDPATGGLKFD
                     AMLAALRGLPPKSIVLLHACCHNPTGVDLTRAQWEQLIPVLRDGGLIPYLDLAYQGFG
                     DGIEEDAYAVRALASAGVSFFVANSFSKSMSVYGERCGALSVVCPNAHEAGLVLGQLK
                     FTVRRNYSSPPIHGGQLVATVLTDPGLRENWERELGAMRERILAMRARLHGVLAAKLP
                     GRDFGYFLTQRGMFSYTGMTAAQADTLREVHGVYMLRSGRICVAGLNNANVEATAVAM
                     AAVLGAG"
     gene            complement(1818..3125)
                     /locus_tag="VFA35_08265"
     CDS             complement(1818..3125)
                     /locus_tag="VFA35_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZT56204.1"
                     /translation="MFCAIDFGTSNSAIAIPAGADAAAGMRLVELEPGHRTMPTAVFY
                     FADDTRAGPERDGPPRAFGRAAVAAYVDGLDGRLMRSMKSILGSHLIDQTTDVGGGRG
                     AKYLDIVAGYLKHLKGRAERQAGAPIRRAVLGRPVFFVDDDPVRDAQAQAALETAARQ
                     VGFDEVQFQYEPIAAAFDYERSVAREQIVLVADIGGGTSDFSVVRVGPQRIDLPDRKR
                     DILANHGVHVAGTDFDRRIELAAILPAYGYGALGPSVAGAPAREVPSGVYFDLATWHL
                     INTVYSPGRVAELRGMRGFYAEPKHHERLMVVVDERLGHELMARAEAAKIDVANGGAT
                     RIDLGHTERHLAAALTERQAVDAIEVDTRRIVDAARAAVAQAGLQPAQVDALYFTGGS
                     TGLRLLASRIEAVFPAAKAVHGDRFASVATGLGLHAQRWFARG"
     gene            complement(3210..>3401)
                     /locus_tag="VFA35_08270"
     CDS             complement(3210..>3401)
                     /locus_tag="VFA35_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56205.1"
                     /translation="RRHRQRERVMAFGVRRTLVWATIAAAIGGAVMLASSGAGLPRLD
                     TSARPGAAPAGTATPLQLH"
BASE COUNT          500 a         1197 c         1222 g          482 t
ORIGIN      
        1 tcgcgcgcag cgacaagcat tgagcgcacc ggccgccggg cgcggccgcg gcgctacgat
       61 ggcatcccac cgtgcccttg cggcacccat cgagaggcat tcgccatggc caacccaccc
      121 accagcatct acgatttcga cgcggtctcg atcgagggcc ggccggcgca gctttcgacc
      181 cagcgcggca aggtcatgct gatcgtcaac accgcgagcg cctgcggctt cacgccccag
      241 ttcgccgggc tcgaagcgct gtggaagacc tatgccgaca agggcctggt cgtgatcggc
      301 tttccgagca acgagttcgg cggccaggac ccgggcagcg acggcgagat cgcgtcgttc
      361 tgccagctga actacggcgt gagctttccg atgatgagca aggtcaaggt caacggcgcg
      421 caggcgcacc cgctgtggca gtggctgacg aaggaagcgc cgggctttct cggcagcacc
      481 tcggtgaaat ggaacttcac caagttcctg gtcggccgcg acggccaggt actcaagcgc
      541 tatgcgccga ccgacaaacc cgaatcgatc aagcgcgaca tcgaagcggc gctggccgcc
      601 tgaccccatg ttcgaacaca ttcctccctt cgccggcgac ccgatcctga gcctgaacga
      661 ggacttccag aaagacccgc ggccgaacaa gatcaacctg agcatcggaa tctacttcga
      721 cgacgccggc cgcctcccgg tgatggacgc ggtgcggcgc gccgaagcga tcgtcgtcgc
      781 caaaggcggc ccgaagccct acctgccgat cgaaggcgcg gcgaacttcc gcgccgccgt
      841 gcagcagttg ctgttcggcg ctggccacga ggcgatcacg tcgggccggg tcgcgacgat
      901 ccagtcggtg ggctcgagcg gcgggctcaa ggtgggcgcc gatttcctgg cgcgttactt
      961 cccgggcagc caggtctggg tcagcgaccc gacctgggac aaccaccgct cgatgttcga
     1021 gggcgcgggc ctgacggtga acgcctaccc gtactacgac ccggccaccg gggggctgaa
     1081 gttcgacgcg atgctcgcgg cgctgcgtgg cctgccgccg aaaagcatcg tgctgctgca
     1141 cgcctgctgc cacaacccga ccggcgtcga cctgacacgt gcgcagtggg agcagctgat
     1201 cccggtgctg cgcgacggcg gcctgattcc ctacctcgat ctcgcgtacc agggcttcgg
     1261 cgacggcatc gaggaagacg cctacgcggt gcgcgcgctc gcgagtgccg gcgtgagctt
     1321 cttcgtcgcg aactcgttct cgaagagcat gagcgtgtac ggcgagcgct gcggcgcgct
     1381 cagcgtggtc tgcccgaacg cgcacgaggc cgggctggtg ctgggccagc tgaagttcac
     1441 ggtgcggcgc aactactcga gcccgccgat ccacggcggc cagctcgtgg cgaccgtgct
     1501 gaccgacccc ggcctgcgcg agaactggga gcgcgagctc ggcgcgatgc gcgagcgcat
     1561 cctcgcgatg cgcgcgcggc tgcacggcgt gctcgcggcc aagctgccgg gccgcgactt
     1621 cggctacttc ctgacccagc gcggcatgtt cagctacacc ggcatgaccg ccgcgcaggc
     1681 cgacaccttg cgcgaggtgc acggcgtcta catgctgcgc tccgggcgca tctgcgtcgc
     1741 cggcttgaac aacgccaatg tcgaggcgac ggcggtggcg atggcggcgg tgctcggcgc
     1801 cggctgaacg cgcgcggtca gccgcgcgcg aaccagcgct gcgcgtgcag gccaaggccg
     1861 gtcgcgacgc tggcgaagcg gtcgccgtgc acggccttcg cggccgggaa caccgcttcg
     1921 atgcggctcg cgagcagccg cagcccggtc gagccgccgg tgaagtacag cgcatcgacc
     1981 tgcgccggct gcagcccggc ctgcgcgacc gccgcgcgcg ccgcgtcgac gatgcgccgg
     2041 gtgtcgacct cgatcgcgtc gaccgcctgc cgctcggtca gcgccgccgc gaggtggcgt
     2101 tcggtgtggc cgaggtcgat gcgcgtcgcg ccgccgttgg ccacgtcgat cttggcggct
     2161 tcggcgcgcg ccatcagctc gtggccgagg cgttcgtcga ccacgaccat cagccgctcg
     2221 tggtgcttcg gctcggcgta gaagccgcgc atcccgcgca gctcggcgac gcggccgggg
     2281 ctgtagacgg tgttgatcag gtgccaggtc gcgaggtcga agtacacgcc gctcggcacc
     2341 tcgcgcgcgg gcgcgccggc cacactcggg ccgagcgcgc cgtagccata ggcaggcagg
     2401 atcgcggcga gctcgatgcg tcggtcgaaa tcggtgccgg ccacgtgcac gccgtggttc
     2461 gcgaggatgt cgcgcttgcg gtcgggcagg tcgatgcgct gcggcccgac ccgcacgacc
     2521 gagaagtcgg aggtgccgcc gccgatgtcg gcgaccagca cgatctgctc gcgcgccacg
     2581 ctgcgttcgt agtcgaacgc agcggcgatc ggctcgtact ggaactggac ctcgtcgaag
     2641 ccgacctggc gcgccgcggt ttcgagcgcg gcctgcgcct gcgcgtcgcg caccgggtcg
     2701 tcgtcgacga agaacaccgg ccggccgagc accgcgcggc ggatcggcgc gccggcttgg
     2761 cgctcggcgc ggcccttcag gtgcttcagg tagccggcga cgatgtcgag gtacttggcg
     2821 ccgcggccgc cgccgacgtc ggtcgtctgg tcgatcaggt ggctgccgag gatgctcttc
     2881 atcgagcgca tcagccggcc gtcgaggccg tcgacatacg cggcgacggc ggcgcggccg
     2941 aaggcgcgcg gcgggccgtc gcgttccggc cccgctcggg tgtcatccgc gaaatagaac
     3001 accgccgtcg gcatcgtccg gtggccgggt tcgagctcga ccaggcgcat gccggccgcg
     3061 gcatcggcgc cggccggaat ggcgatcgcg gaattggacg tgccgaagtc gatggcgcag
     3121 aacatgggcg ggagccgccg gcggcgggca cggcggcgtg tggggcgggg gcgcgattct
     3181 acggaggaac ccgcgctcgc gcggggccgt caatgaagct gcagtggcgt ggccgtgcct
     3241 gccggggcgg cgcctggccg cgcagacgtg tccaggcgcg gcagacccgc gccgctcgac
     3301 gccagcatca cggcgccgcc gatcgccgcc gcgatcgttg cccacaccag cgtcctgcgc
     3361 actccgaagg ccatcacccg ctccctctgt cgatggcgcc g
//
LOCUS       DASRDG010000492         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1225900, whole genome shotgun sequence.
ACCESSION   DASRDG010000492 DASRDG010000000
VERSION     DASRDG010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225900"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFA35_08275"
     CDS             complement(<1..294)
                     /locus_tag="VFA35_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZT56206.1"
                     /translation="MTIDFSGIWVPLVTPFAADGSVDHAALRALVKRCADAGIAGLVA
                     LGTTGEPSALDQAEQDAVFTTILDAAGAKLPVIAGLAGNHAPGLLARAKQLSAL"
     gene            complement(544..2763)
                     /locus_tag="VFA35_08280"
     CDS             complement(544..2763)
                     /locus_tag="VFA35_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZT56207.1"
                     /translation="MHPLMHQAIQSSILTVGTGLTRRAVLAGGAGTGLLIAVGGFAPH
                     AASAAATGAEGVATGPLAPNQFVRVGTDNLVTIVCKHHEMGQGNATGLASMAADELDA
                     DWALVRTEFAPADVKLYANLAFGMQGTGGSSAIANSYLQMRSAGATARAMLVAAAAEA
                     WKVPASEVKASKSVLSHASGKRATYGEMAAAAAKQTPPANPTLKTPAQFTLMGKAGAT
                     PRVDSVEKCNGSATYTIDVKLPGLLTAVIAWPPSFGAKLVSFDATDAKKVKGVTDVVQ
                     VPEGVAVVAKGTWAALQGRRALKVVWDEAESGKLDSDALLAQYKAQAATPGKPFARPA
                     KDAPAPAPVAKTVEAVYEFPFLAHATMEPMNCVAWLHDGMLETWSGHQFPTFDHMAAA
                     QAAGLPLEKVKLHTLLSGGSFGRRANAWSDFTVAAVNVAKAIGARAPVRLQYTREDDM
                     GAGLYRPMYVHAVKAGIDAQGRVAGWQHTVVGQSIVTGTAMEMLVKDGIDPTSVEGVS
                     PTPYALTMLSADLHSPVLAVKPLWWRSVGHTHTAYVMETMMDELARAAGKDPLAFRLA
                     LLDKSERAAGVLRLAADKAGWGKPTRSGVAQGIAMEESFGTYVAQVAEVSMQGGKVKV
                     ERVVCAVDCGVAVNPDVVRAQMEGCIGFALGALYYGEIELKNGRAAQRNFDTYRSLRI
                     HEMPKVEVYIVPSTAAPTGVGEPGVPPLAPAVANAVAKLGGPTVRRLPFARAGIVAA"
     gene            complement(2780..>3088)
                     /locus_tag="VFA35_08285"
     CDS             complement(2780..>3088)
                     /locus_tag="VFA35_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZT56208.1"
                     /translation="DGETMRSCAAPVGAMAGRKIVTIEGLSSRAAKAVQKAWVDLDVV
                     QCGYCQSGQIMSAAVLLEQKPRPTDADIDQAMAGNICRCATYQRIRAAIHAAAKQLAA
                     "
BASE COUNT          412 a         1108 c         1096 g          472 t
ORIGIN      
        1 cagcgcgctc aactgcttcg cacgcgccag cagacccggc gcgtggttgc cggcgaggcc
       61 cgcgatcacc ggcagcttgg cgccggccgc gtccaggatc gtcgtgaaga ccgcgtcctg
      121 ctcggcctgg tccagcgccg acggctcgcc cgtcgtgccg agcgcgacga ggccggcgat
      181 gccggcgtcg gcgcagcgct tcacgagcgc gcgcaaggcc gcgtgatcga ccgaaccatc
      241 cgccgcgaac ggcgtgacca gaggcaccca gatgccggag aaatctatcg tcatcgcttc
      301 cactcctgaa cgaccgaacg gtggaaaccg tcagcggcgt gcgggcagga gggcggtgaa
      361 gtcgggggaa gagaagagct ctgctgcggt tccgtcacac acgctgacgg aacggcgagc
      421 tccggtcagg cgagccgctg tttttgtttg gacttggctg cgcgcgcgca cccgcgcgcg
      481 gccgcgaggc ggtcgaaggc gggtggctgc gagcggtcca tgcggcggag tctataggag
      541 ccttcaggcc gcgacgatcc ccgcccgcgc gaacggcagc cggcgcacgg tcggcccgcc
      601 gagcttcgcg accgcgttgg cgaccgcggg cgcgagcggc ggcacgccgg gttcgcccac
      661 gccggtgggc gcggccgtgc tcggcacgat gtagacctcg accttgggca tctcgtggat
      721 gcgcagtgac cggtaggtgt cgaagttgcg ctgcgcggcg cggccgttct tcaactcgat
      781 ctcgccgtag tagagcgcgc ccagcgcgaa accgatgcag ccttccatct gcgccctgac
      841 cacgtcgggg ttgacggcga cgccgcagtc gaccgcgcag accacgcgct cgaccttgac
      901 cttgccgccc tgcatcgaca cctcggcgac ctgcgcgacg tacgtgccga acgactcttc
      961 catcgcgatg ccttgcgcca cgccggagcg cgtcggcttg ccccagccgg ccttgtcggc
     1021 cgcgagcctg agcacgccgg cggcgcgctc gctcttgtcg agcagcgcga ggcggaacgc
     1081 gagcgggtcc ttgccggccg cgcgggcgag ctcgtccatc atcgtttcca tcacgtacgc
     1141 ggtgtgcgtg tggccgaccg aacgccacca cagcggcttg accgcgagca cgggcgagtg
     1201 caggtcggcg ctcagcatgg tcagcgcata cggcgtcggc gagacgcctt cgaccgaggt
     1261 cgggtcgatg ccgtccttga ccagcatctc catcgcggtg ccagtaacga tcgactggcc
     1321 tacgaccgtg tgctgccagc cggcgacgcg gccctgcgca tcgatgccgg ccttcaccgc
     1381 atgcacgtac atcggccggt acagccccgc gcccatgtcg tcctcgcggg tgtactgaag
     1441 ccgcaccggc gcgcgagcgc cgatcgcctt cgcgacgttg acggccgcga ccgtgaagtc
     1501 ggaccacgcg ttcgcgcgcc ggccgaagct gccgcccgag agcagcgtgt gcagcttgac
     1561 cttctccagc ggcagccccg cggcctgtgc ggcggccatg tggtcgaagg tcggaaactg
     1621 gtggccgctc caggtttcga gcatgccgtc gtgcagccac gcgacgcagt tcatcggttc
     1681 catcgtcgcg tgtgcgagga acgggaactc gtagaccgcc tcgaccgtct tcgcgacggg
     1741 tgccggtgcc ggcgcgtcct tcgccggccg tgcgaacggc ttgccgggcg tcgcggcctg
     1801 cgccttgtac tgcgcgagca gcgcgtcgga atcgagcttg ccgctttcgg cttcgtccca
     1861 caccaccttc agcgcgcgcc gcccctgcag cgcggcccag gtgcccttgg cgacgaccgc
     1921 gacgccctcg ggcacctgca ccacgtcggt cacgcccttg accttcttcg cgtcggtcgc
     1981 gtcgaacgac accagcttcg cgccgaacga cggcggccac gcgatcaccg cggtcagcag
     2041 ccccggcagc ttgacgtcga tcgtgtaggt ggcgctgccg ttgcacttct cgaccgagtc
     2101 gacgcgcggg gtcgcgccgg ccttgcccat cagcgtgaac tgcgcgggcg tcttcaacgt
     2161 cgggttcgcc ggcggggtct gcttcgcggc ggcggcggcc atctcgccgt aggtcgcgcg
     2221 cttgcccgag gcgtggctga gcacgctctt cgaggccttg acctcgctcg ccggcacctt
     2281 ccaggcctcg gccgcggcgg ccaccagcat cgcgcgcgcg gtcgcgccgg cgctgcgcat
     2341 ctgcaggtac gagttcgcga tcgccgacga gccgccggtg ccctgcatgc cgaacgcgag
     2401 gttggcgtag agcttcacgt ccgccggcgc gaactcggtg cgcaccagcg cccagtcggc
     2461 gtcgagctcg tcggccgcca tcgaggccag gccggtcgcg ttgccctggc ccatctcgtg
     2521 gtgcttgcag acgatggtga cgaggttgtc ggtgcccacg cgcacgaact ggttcggcgc
     2581 gagcgggccg gtggccacgc cctcggcgcc ggtcgcggcg gcggacgcgg cgtgcggtgc
     2641 gaaaccgccg accgcgatca gcaggccggt gccggcgccg ccggcgagca cggcgcggcg
     2701 ggtcaggccc gtgccgacgg tcaggatgga ggattggatc gcttggtgca tcaacgggtg
     2761 catggcgtgt gcctcctggt caagcggcga gctgcttagc cgccgcgtgg atcgcggcgc
     2821 ggatgcgctg gtaggtggcg cagcggcaga tgttgccggc catcgcctgg tcgatgtcgg
     2881 cgtcggtcgg cctgggcttc tgctcgagca gcaccgcggc cgacatgatc tggcccgact
     2941 ggcagtagcc gcactgcacc acgtcgagat cgacccaggc cttctgcacc gccttcgcgg
     3001 cgcgcgacga gagaccttcg atcgtgacga tcttgcgccc ggccatggcc ccgaccggcg
     3061 cggcgcacga gcgcatcgtc tcgccgtc
//
LOCUS       DASRDG010000493         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_528799, whole genome shotgun sequence.
ACCESSION   DASRDG010000493 DASRDG010000000
VERSION     DASRDG010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528799"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /gene="ftsL"
                     /locus_tag="VFA35_08290"
     CDS             complement(<1..200)
                     /gene="ftsL"
                     /locus_tag="VFA35_08290"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HZT56209.1"
                     /translation="MTRLNVLLLIVLIFSSIYLVRVSHESRRLFADLDKARSEEHLLD
                     SDFEKLKAEKQAQATPLRVEKTA"
     gene            complement(197..1144)
                     /gene="rsmH"
                     /locus_tag="VFA35_08295"
     CDS             complement(197..1144)
                     /gene="rsmH"
                     /locus_tag="VFA35_08295"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZT56210.1"
                     /translation="MPTEHELQHTTVLLNEAIDALVTTPDGIYVDGTFGRGGHSGLLL
                     SRLSPKGRLVAIDRDPDAIAAATSGETRVTDPRFSIHHTAFAHMVPTLAALGIDKVDG
                     LLLDLGVSSPQIDDPERGFSFRFDGPLDMRMDTTRGESAADFLARADVRQMTEVIRDY
                     GEERFALQVAKALVARRESGNPVRTTGELSSVVAGAVKTREAGQNPATRTFQALRIFV
                     NAELEELEQALSAALDLLLPGGRLVVISFHSLEDRIVKTFIARESKDVIDRRAPFAAP
                     KPMRLKSIARVKPSDAEVDANPRARSAVMRVAERTEVAA"
     gene            complement(1152..1580)
                     /gene="mraZ"
                     /locus_tag="VFA35_08300"
     CDS             complement(1152..1580)
                     /gene="mraZ"
                     /locus_tag="VFA35_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZT56211.1"
                     /translation="MFQGPAALTLDGKGRIAVPARHRELLTAMGCHQLTITKHPEGSL
                     MIFPRPAWETFRDKVSALPMEAAGWKRVFLGNAMDVDIDGSNRVLIAPELRASAGLVR
                     DVMLIGMGSHFELWDAQRHAEHEAKVMQSEMPESLKSFSF"
     gene            complement(1880..>2182)
                     /locus_tag="VFA35_08305"
     CDS             complement(1880..>2182)
                     /locus_tag="VFA35_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZT56212.1"
                     /translation="ALYRRALRGFRDALSELRAGLHLTDPPPAADAMAHRMHDLQGLA
                     GTVGARLLQAAARDLHRALALGDHDATHAGRARVDAAGRDALREVDRMLAKDAPAP"
BASE COUNT          385 a          715 c          717 g          365 t
ORIGIN      
        1 gcggtcttct cgacgcgcag cggcgtcgcc tgcgcctgct tctcggcttt cagcttctcg
       61 aaatcgctgt cgagcaggtg ctcttcgctg cgtgccttgt cgaggtcggc gaacaagcgg
      121 cgcgactcgt gcgagacgcg cacgaggtag atcgagctga agatcagcac gataagcagc
      181 agcacgttca ggcgcgtcat gccgcgacct ccgtgcgctc ggcgacgcgc atcacggccg
      241 agcgcgcacg cggattcgcg tcgacctcgg cgtcgctcgg cttcacgcgt gcgatcgact
      301 tcagccgcat cggcttgggt gccgcgaagg gcgcgcggcg gtcgatcacg tccttgctct
      361 cgcgcgcgat gaaggtcttg acgatgcggt cttcgagcga gtgaaagctg atcacgacca
      421 gccgcccgcc cggcagcagc agatccagcg ccgcgctcag ggcttgttcg agctcctcaa
      481 gctcggcgtt gacgaaaatc cgaagagctt gaaatgtgcg cgttgcaggg ttctggcccg
      541 cttcgcgggt cttgaccgca ccagccacga cgctggaaag ctcgcctgtg gttcgaacag
      601 ggttcccgct ttcgcgccga gcaacaagcg cctttgcaac ctgtagagca aaccgttctt
      661 ccccatagtc acgtatcacc tccgtcattt ggcgcacgtc ggcgcgggcg agaaagtcgg
      721 ccgcgctttc gccgcgggtc gtgtccatgc gcatgtccag cgggccgtcg aaacggaagc
      781 tgaagccgcg ctcggggtcg tcgatctgcg gcgacgacac gcccagatcg agcagcaagc
      841 cgtcgacctt gtcgatgccg agcgcggcga gcgtgggcac catgtgcgca aaagcggtgt
      901 gatgaatgga aaaacgcggg tcagtcaccc gcgtttcgcc tgaagtcgcc gccgcaatgg
      961 catcggggtc ccggtcgatc gccaccaacc ttcctttcgg cgacaggcgc gacagcagca
     1021 gccccgagtg cccgccgcga ccgaaggtgc cgtccacgta gatgccgtcg ggcgtggtga
     1081 cgagcgcatc gatggcctcg ttcagcaaca cggtggtgtg ctgcagttcg tgctcggtcg
     1141 gcatggtgcg gtcagaagct gaagctcttc agcgactcgg gcatctcgga ctgcatgacc
     1201 ttggcctcgt gctcggcgtg gcgctgcgcg tcccagagct cgaagtggct gcccatgccg
     1261 atcagcatca catcgcgaac caggccggcc gacgcgcgca gctcgggcgc gatcagcacg
     1321 cggttggagc cgtcgatgtc gacgtccatc gcgttgccga ggaagacgcg cttccagccg
     1381 gccgcctcca tgggcagcgc cgagaccttg tcgcgaaagg tttcccacgc cggtcgcgga
     1441 aagatcatca gcgagccttc cgggtgcttc gtgatcgtca gctggtggca gcccatcgcc
     1501 gtcaacagct cgcggtgacg cgccggcacg gcgattcgcc ccttgccgtc caacgtcaga
     1561 gccgcaggcc cttgaaacac gatgtttgcc acaaaaaccc agaaaagtgc accttgcgct
     1621 actttaacgg atcgcggagc caccggtcaa ccaaatcgtc gaaaaagtca atcgaatcaa
     1681 agacttagcg ctggtttgaa acacagtatg cggggtaaaa tgtgttcaaa aacaaagacc
     1741 tgcataaccc tgtgaaagtg atgggtgaga ttcgacgggc gcagcgtaac gcgcgggtcg
     1801 tgaagaagat cacgcatgcc gcgcaacagg cggtcaggat ggaagccggc gcggtctcgt
     1861 cgcgggccgg gccagcctcc tacggggccg gcgcgtcctt cgccagcatg cggtcgacct
     1921 cgcgcagtgc atcgcgcccc gcggcgtcga cgcgagcgcg gccggcgtgc gtcgcgtcat
     1981 gatcgcccag cgccagcgcg cgatgcaggt ctcgcgctgc ggcctgcaac agccgtgcgc
     2041 cgacggtgcc cgcgaggccc tgcagatcgt gcatccggtg cgccatcgcg tcggccgccg
     2101 ggggcgggtc ggtcaggtgc agtcccgcgc gcagctcgga caatgcgtcc cgaaagcccc
     2161 tcagcgcgcg ccgatagagc gc
//
LOCUS       DASRDG010000494         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1585583, whole genome shotgun sequence.
ACCESSION   DASRDG010000494 DASRDG010000000
VERSION     DASRDG010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585583"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..829
                     /locus_tag="VFA35_08310"
     CDS             62..829
                     /locus_tag="VFA35_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZT56213.1"
                     /translation="MSTATLAYRLTGSTGMHRGDRAYQQDQVEIVPHERIPGCLMAVL
                     ADGMGGKSGGRKAADQVLLTANQLFERYAPSRDDAGEALKQLVLEAHLMIKLTAITAE
                     EEPHSTVAAFIVGPSRACDIIHAGDSRVYHFRGAELVSRTVDHSYVQRLVEEGEITED
                     EANTHPQSNLLTGCLGTNADPPLAHARVAQLEVGDSLLACSDGLWHYFTPRELGAIVH
                     ALPPREASEMLVNKARQRARGAGDNLSLALVRVEALE"
     gene            complement(846..1463)
                     /locus_tag="VFA35_08315"
     CDS             complement(846..1463)
                     /locus_tag="VFA35_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56214.1"
                     /translation="MTRRLLGFALLCGISFGAVADREHDRIASDRVAADAKLAAQEHA
                     CATRFVVASCVEDARTAHREALNKLRQQELQLDEGRRRAAAEARRNAIAEKAQAQQAR
                     ASEATNDAPRVRVRRAPAAASAAVDRPDAGAAPHRPSASANANGKATAGADRSAVEQR
                     HRESFEARQREAQAHRDAVLRRNAERAAKGKVAAPLPPGGASAPH"
     gene            complement(1460..2557)
                     /gene="zapE"
                     /locus_tag="VFA35_08320"
     CDS             complement(1460..2557)
                     /gene="zapE"
                     /locus_tag="VFA35_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HZT56215.1"
                     /translation="MGVRALYEQTLAERGYTADPAQLRALQALERCENEWIDYKARRS
                     NALAKLIARPPIPRGVYMYGGVGRGKSFLMDCFFNSVPLTRKTRLHFHEFMREVHREL
                     SELQGTVNPLAELGKRIARRYRLICFDEFHVSDVTDAMILYRLLEALFDNRVSIVTTS
                     NFKPDDLYPNGLHRDRILPAIALLNQKLEVLNVDNGNDYRQITLAQVGLYQTPLDAAA
                     EAAMTDAFERLAEAKDESALLHIEHREIRARRRAGGVVWFDFKELCGGPRSQNDYLEL
                     ASQFHTLLLSGVPAMSPRMASEARRFTWLVDVLYDRRVKLIMSAETEPEGLYTEGPMA
                     HEFPRTVSRLREMQSAPFLALSRRDVDTSLT"
     gene            complement(2562..>3272)
                     /locus_tag="VFA35_08325"
     CDS             complement(2562..>3272)
                     /locus_tag="VFA35_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT56216.1"
                     /translation="KIELGVKVGKVDTGGAGVSVSYTDAKGAEQVLAVDKLSVSIGRV
                     ANTIGLHAEAVGLKLDERGFIAVDADCRTNLPNVWAIGDVVRGPMLAHKAEEEGVAVA
                     ERIAGQHPHVDFNTVPWVIYTSPEIAWVGQTEQQLKAAGRAYKAGTFPFMANGRARAL
                     GDTTGMVKMLADAKTDEILGVHMVGPMVSELIAEAVVAMEFKASSEDIARICHAHPSL
                     SEATKEAALAVDKRTLNF"
BASE COUNT          481 a         1142 c         1117 g          532 t
ORIGIN      
        1 ctcggattct gtgcggaatt gggcgacgtg agcgaatgca aagcgattat agttttcgat
       61 catgagcaca gcgaccttgg cttaccgctt gaccggcagc accggcatgc accgcggcga
      121 ccgggcctac cagcaggacc aggtcgagat cgtgccgcac gagcgcattc cgggctgcct
      181 gatggcggtg ctcgccgacg gcatgggcgg caagagcggc gggcgcaagg ccgccgacca
      241 ggtgctgctg accgcgaacc agctgttcga gcgctacgcg ccgagccgcg acgatgccgg
      301 cgaggcgctg aagcagctcg tgctcgaagc ccacctgatg atcaagctga ccgcgatcac
      361 cgccgaagag gagccgcaca gcacggtggc agcgttcatc gtcggcccct cgcgagcctg
      421 cgacatcatt cacgcgggcg actcgcgcgt gtatcacttt cgcggtgccg agctggtcag
      481 ccgcaccgtc gatcattcgt atgtgcagcg cctggtcgaa gaaggcgaga tcaccgaaga
      541 cgaagccaac acgcacccgc agtcgaacct gctgaccggc tgcctcggca ccaacgccga
      601 cccaccgctg gcgcacgcgc gcgtcgccca gctcgaggtc ggcgacagcc tgctcgcctg
      661 cagcgacggc ctgtggcact acttcacgcc gcgcgaactc ggcgcgatcg tgcatgcgct
      721 gccaccgcgc gaggccagcg agatgctggt caacaaggcg cgccagcgcg cgcgcggtgc
      781 cggcgacaac ctgtcgctgg cgctggtgcg cgtcgaagcg ctggagtagg cgcgctcaca
      841 ccttctcaat gcggcgccga ggcgccgccc ggcggcaacg gcgccgcgac cttgcccttt
      901 gccgcgcgct cggcattgcg gcgcagcacc gcgtcgcggt gcgcctgggc ctcgcgctgg
      961 cgcgcctcga agctctcgcg atggcgttgc tcgacggcgc tgcggtcggc gccggctgtg
     1021 gctttcccgt tcgcgtttgc gctcgcgctt ggacgatgcg gtgccgcacc cgcatcgggt
     1081 cgatccaccg cagcggatgc ggccgcaggc gcccgccgca cgcgcacccg cggcgcgtcg
     1141 ttcgtcgcct cgctggcccg cgcttgctgg gcctgcgcct tctcggcgat cgcgttgcgg
     1201 cgcgcctcgg cggccgcgcg gcgccggccc tcgtcgagct ggagttcttg ctggcgcagc
     1261 ttgttcagcg cttcgcggtg cgcggtgcgc gcgtcctcga cacagctggc gacgacgaat
     1321 cgcgtcgcac acgcatgctc ctgcgcggcg agcttcgcgt ccgccgcaac gcggtcgctc
     1381 gcgatgcggt catgctcgcg atcggccact gcaccgaacg agataccgca cagcagcgcg
     1441 aaccccagca ggcgacgtgt catgtcagcg aagtatccac gtcgcgccgc gacagcgcca
     1501 gaaacggggc cgactgcatc tcgcgcaggc gcgacaccgt gcgcgggaac tcgtgcgcca
     1561 tcggcccctc ggtgtacagc ccctcgggtt cggtctcggc cgacatgatc agcttgacgc
     1621 gccggtcgta gagcacgtcg accagccagg tgaagcggcg cgcctcggag gccatgcgcg
     1681 gcgacatcgc cggcacgccc gacagcagca gcgtgtggaa ctggctcgcg agctcgaggt
     1741 agtcgttctg cgaacgcggg ccaccgcaca gttccttgaa gtcgaaccac acgacgccgc
     1801 cggcgcgacg ccgtgcgcga atctcgcggt gttcgatgtg cagcagcgcc gattcgtcct
     1861 tggcctcagc gagacgctcg aacgcgtctg tcatggccgc ctcggccgcg gcgtccagcg
     1921 gggtctggta cagcccaacc tgcgccagcg tgatctgccg gtagtcgttg ccgttgtcga
     1981 cgttcagtac ttcgagcttc tggttcagca gcgcgatcgc cggcaggatg cggtcgcggt
     2041 gcaggccgtt cgggtagagg tcgtcgggct tgaaattcga ggtcgtgacg atgctgacgc
     2101 ggttgtcgaa cagcgcttcg agcagccggt acaggatcat cgcgtcggtc acgtcggaga
     2161 cgtgaaactc gtcgaagcag atcagccggt agcgccgcgc gatgcgtttg ccgagttcgg
     2221 ccagcgggtt caccgtgccc tgcagctcgc tcagctcgcg gtgcacctcg cgcatgaact
     2281 cgtggaagtg cagccgcgtc ttgcgcgtca gcggcaccga gttgaagaag cagtccatca
     2341 ggaagctctt gccccggccc acaccgccgt acatgtacac gccgcgcggg atcggcggcc
     2401 gcgcgatcag cttggccagc gcgttgctgc ggcgggcctt gtagtcgatc cactcgttct
     2461 cgcagcgctc gagcgcttgc agcgcccgca actgcgcggg gtcggccgtg tagccgcgct
     2521 cggcgagggt ctgctcgtag agagcgcgga cgcccacggc gttagaaatt caaggtccgc
     2581 ttgtcgacgg cgagggccgc ttccttggtc gcctcggaca gcgacgggtg cgcgtggcag
     2641 atccgcgcga tgtcttccga cgaggccttg aactccatcg cgacgaccgc ttcggcgatc
     2701 agctccgaga ccatcggccc gaccatgtgc acgccgagga tctcgtcggt cttcgcatcg
     2761 gcgagcatct tgaccatgcc ggtcgtgtcg cccagcgcgc gcgcgcggcc gttcgccatg
     2821 aacgggaagg tgccggcctt gtaggcgcgg cccgcggcct tcagctgctg ctcggtctgg
     2881 ccgacccagg cgatctcggg cgaggtgtag atcacccacg gcacggtgtt gaagtcgacg
     2941 tgcgggtgct ggccggcaat gcgctcggcg accgcgacgc cctcttcctc ggccttgtgc
     3001 gccagcatcg gcccgcgcac cacgtcgccg atcgcccaga cgttcggcag gttcgtccgg
     3061 cagtcggcgt cgaccgcgat gaagccgcgc tcgtcgagct tcaggccgac cgcttcggca
     3121 tgcaggccga tcgtgttggc gacgcggccg atcgagacgc tcagcttgtc gaccgccagc
     3181 acctgttcgg cgcccttggc gtcggtatag gacacgctga cgccggcacc gccggtgtcg
     3241 accttgccga ccttgacgcc cagttcgatc tt
//
LOCUS       DASRDG010000495         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1226346, whole genome shotgun sequence.
ACCESSION   DASRDG010000495 DASRDG010000000
VERSION     DASRDG010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226346"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1118)
                     /locus_tag="VFA35_08330"
     CDS             complement(93..1118)
                     /locus_tag="VFA35_08330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZT56217.1"
                     /translation="MKVSVIVPCRNERAYIEGFCAGVLRQRLAPGWQLQLVIADGESD
                     DGTRELLQRLAAADARIVWIANPARIVSAGLNSALKLATGEVIVRMDVHSEYADDYIA
                     QCLAVLAETGADNVGGPWRAVPDADGGAMQHAVAAAFQSRWVAGGALSRQLDYDGPAD
                     TVYLGSWPRASFERFGGFDEALVRNQDDEHNLRIVKGGGRVWQSSRIRSVYRPRATLR
                     QVFRQYLQYGYWKPFVMKKHGQPASLRHAVPGVFVAGLLLSAALALAGLTAWPLLALA
                     GLYALGVLAMTAAIAASQPVAWPALWRIPAVIAAYHVGYGIGSIIGGWDALRHERGRA
                     RFGTLTR"
     gene            complement(1115..2257)
                     /locus_tag="VFA35_08335"
     CDS             complement(1115..2257)
                     /locus_tag="VFA35_08335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZT56218.1"
                     /translation="MTAPLRVLLVCDRLDIAGGVERFVCALANHLANEGMDVAIGSVD
                     TSRAEIRYPLSSAVRVLSAAGRRAELVADARPWAARAWALAWTQWRIGRSLAALIRSE
                     RPDVVVLNGLTTACSVLAIDRTIAACAICCDHNHFSARSAPWRRLRQWLYPKVAAVVC
                     LTRADASRFRALNPNTRVIYNASSLRADAPALPEHGPALAVGRHVAQKGFDLLVRAWA
                     VVAQRFPQARLCVVGDGPLRAEHEALAQSLGIGDRIDWQMPTAQIEHHYREAAVFVLP
                     SRYEGMPLALLEAQAMGVPAVAFDCPTGPAEIISAQTGLLVPPGDVAALAAALIELLS
                     APALREQMAFAAIERSRELFSPQEHDRRWTALVREVGASAGVGASA"
     gene            complement(2254..3189)
                     /locus_tag="VFA35_08340"
     CDS             complement(2254..3189)
                     /locus_tag="VFA35_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56219.1"
                     /translation="MGIDIHNLNLLAHAQDLGVRFDRTVAIGRQALFIEDFELEQYRR
                     LRGLAPLVEPAIAPGGPRYFEPLATQWLGARVADAVDASAYENARLIHDMNLPWPADS
                     AERGAYDAVLDFGCLEHVFNFPVAWRNCIDLCRVGGHVLHALPANNLSGHGFYQFSPE
                     LFFNLYRPENGFELRGLYFAMKADARHWWKVANPLDVKRRVNLCNSHEVYMLVIARKL
                     REPRLLPAPQQSDYAEDEWLRAPSTASASVAAPAASSTARRALVRWLARCGLIDAVRA
                     GRARWRVLRDSGLSLPAPDYQRVDVRALIDKPRTA"
     gene            complement(3205..>3902)
                     /locus_tag="VFA35_08345"
     CDS             complement(3205..>3902)
                     /locus_tag="VFA35_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56220.1"
                     /translation="WLRRDFAGARTSALWARAEARAGLRLGWTMMPVAIAGAAYSFAL
                     PAAASLQMARTELGTYFMADRIVRALLNAAEPVFSVVYPRIVTLFGTSARAAFRYAAA
                     WAGCGAIAGVALLGIGHLIWPAIEPLIVSRAGGLDVEGVRAVLRILGWLVPLLLGWKF
                     IGYWILGSGRFDAAYRTCVIVGGIVGVLAAATFGGAAGATGLAWTALGAEAVVIAVAV
                     GGVLLTRRERARA"
BASE COUNT          628 a         1431 c         1365 g          478 t
ORIGIN      
        1 gcatcgtgcg gcgcgcggcg cgcgtagcgc tcgcgcagcg cctgcggctc ggccggttcg
       61 tcgtgcggat tcatggtcgc cgtctcgcag ggtcagcgtg tgagcgtgcc gaagcgcgcg
      121 cggccgcgct cgtgacgcag cgcatcccag ccgccgatga tcgagccgat gccatagccg
      181 acgtgatacg ccgcgatcac cgcaggaatg cgccacagcg cgggccacgc gacgggttga
      241 gaggccgcga tcgccgccgt catcgcgagc acccccagcg catacaggcc ggccagcgcc
      301 agcagcggcc atgccgtgag cccggccagc gcgagcgcgg ccgagagcag cagcccagca
      361 acgaacacgc ccggcacggc gtgccggagc gacgcgggct gaccgtgctt cttcatcacg
      421 aacggcttcc agtagccgta ttgcaggtac tgcctgaaca cctggcgcag cgtcgcgcgc
      481 ggccgataga cgctgcggat gcgcgacgac tgccacaccc ggccgccgcc cttgacgatg
      541 cgcaggttgt gctcgtcgtc ctggttgcgc accagcgctt cgtcgaaacc gccgaagcgc
      601 tcgaagctcg cgcgcggcca actgccgagg tagacggtgt cggccgggcc gtcgtagtcg
      661 agttgccgcg agagcgcgcc gccggccacc cagcgcgatt ggaatgctgc cgccaccgca
      721 tgctgcatcg cgccgccgtc tgcgtccggc accgcgcgcc acggcccgcc gacgttgtcg
      781 gccccggtct cggcgagcac cgcgaggcac tgcgcgatgt agtcgtcggc gtactcgctg
      841 tgcacatcca tgcgcacgat gacctcgccg gtcgcgagct tcagcgcgct gttcagaccg
      901 gccgacacga tccgcgccgg gttcgcgatc cacacgatgc gcgcgtcggc cgcggcgagc
      961 cgctgcagca gctcgcgcgt gccgtcgtcg ctctcgccgt cggcgatgac gagctgcagc
     1021 tgccagcccg gcgcgagccg ctggcgcagc acgcccgcgc aaaagccttc gatgtaggca
     1081 cgctcgttgc ggcacggcac gatgaccgac accttcatgc gctcgctccg accccggcag
     1141 acgcgccgac ctcgcgcacc agcgcggtcc agcgccggtc gtgctcctgc gggctgaaga
     1201 gctcgcggct gcgctcgatc gccgcgaacg ccatctgctc gcgcagggcc ggcgcactca
     1261 gcagctcgat cagcgccgcg gccagcgccg ccacgtcgcc gggtggcacg agcaggccgg
     1321 tctgcgcgct gatgatctcg gccggaccgg tcgggcagtc gaacgcgacg gccggcacgc
     1381 ccatggcctg cgcttcgagc agggccagcg gcatgccttc gtagcgcgag ggcagcacga
     1441 acaccgcggc ctcgcggtag tggtgctcga tctgcgcggt cggcatttgc cagtcgatcc
     1501 ggtcgccgat gcccagcgac tgcgccagcg cttcgtgttc ggcgcgcagc ggcccgtcgc
     1561 cgacgacgca caggcgcgcc tgcgggaacc tctgggcaac caccgcccag gcccgcacca
     1621 gcaggtcgaa ccccttctgc gccacgtgcc gaccgacggc cagcgccggg ccgtgctcgg
     1681 gcagcgcggg cgcatcggcg cgcagcgagg aggcgttgta gatcacgcgc gtgttcgggt
     1741 tcagcgcacg aaaccgcgac gcatcggcac gcgtgaggca gacgacggcg gccaccttcg
     1801 ggtagagcca ctgtcgcagc cgccgccacg gcgccgagcg cgcgctgaag tggttgtggt
     1861 cgcagcagat ggcgcaagcc gcaatcgtgc ggtcgatcgc gagcaccgaa cacgccgtcg
     1921 tcaagccgtt gagcacgacg acatcgggcc gctccgagcg gatcaaggcc gcgagcgaac
     1981 ggccgatgcg ccactgcgtc caagcgagcg cccacgcgcg cgcggcccaa ggccgagcat
     2041 cggcgacgag ctcggcacgg cggcccgccg ccgacaacac gcgcacggcc gaactcaagg
     2101 gatagcggat ctcggcgcgc gaggtgtcga cgctgccgat cgccacgtcc atcccctcgt
     2161 tggccaggtg gttcgccagc gcgcagacga aacgctcgac gccgccggcg atgtcgaggc
     2221 gatcgcacac caacagcacg cgcagcggcg ccgtcatgcg gtccgaggct tgtcgatcaa
     2281 cgcgcgcacg tcgacgcgtt ggtagtccgg cgccggcagg ctcaggccgc tgtcgcgcaa
     2341 cacgcgccag cgcgcgcgac cggcgcgcac ggcgtcgatc aagccgcacc gcgcaagcca
     2401 gcgaaccagc gcccggcggg cggtggacga agcggccggg gctgcgaccg aggccgatgc
     2461 ggtcgacggc gcgcgcagcc attcgtcctc ggcgtaatcg gactgctgcg gcgctggcag
     2521 caacctcggt tcgcgcagct tgcgcgcgat caccagcatg tagacctcgt ggctgttgca
     2581 aaggttgacg cggcgcttca catcgagcgg attggcgacc ttccaccaat gccgcgcatc
     2641 ggccttcatc gcaaagtaca ggccgcgcag ttcgaagccg ttctccggcc ggtagaggtt
     2701 gaagaacagc tcgggcgaga actgatagaa gccgtggccc gacaggttgt tggccggcag
     2761 cgcatgcagc acgtggccgc cgacgcggca caggtcgatg cagttgcgcc aggccaccgg
     2821 gaagttgaac acgtgctcga ggcagccgaa gtcgagcacc gcgtcatagg cgccgcgctc
     2881 ggcgctgtcg gccggccacg gcaggttcat gtcgtggatc aggcgcgcgt tctcgtaggc
     2941 ggaggcatcg acggcatcgg ccacgcgcgc gcccagccac tgcgtcgcca gcggctcgaa
     3001 gtagcgcggg ccgcccggcg cgatcgcggg ttcgaccagc ggcgccagac cgcgcagccg
     3061 gcggtattgc tcgagctcga agtcttcgat gaacagcgcc tggcggccga tcgccacggt
     3121 gcgatcgaag cgcacgccca ggtcttgcgc atgcgccagc aggttgaggt tgtggatgtc
     3181 gatgcccaac gcgcttgttc ctcgtcaggc gcgcgcgcgt tcacgccggg tgagcagcac
     3241 gccaccgacc gccacggcga tgacgaccgc ctcggcgccc agcgccgtcc aggccaggcc
     3301 ggtcgccccg gcagcgccgc cgaaggtcgc tgccgcgagc acgccgacga tgccgccgac
     3361 gatcacgcag gtgcgatagg cggcatcgaa gcggccgcta ccgaggatcc agtagccgat
     3421 gaacttccag ccgagcagca agggcacgag ccagcccaga atccgcaaca ccgcgcgcac
     3481 gccctccaca tcgagaccgc ccgcgcgcga cacgatcagc ggctcgatcg ccggccagat
     3541 cagatgcccg atgccgagca gcgcaacacc cgcgatcgcg ccgcagccgg cccaggcggc
     3601 ggcatagcgg aacgcggcgc gcgcgctggt gccgaacagc gtgacgatgc gtgggtacac
     3661 gaccgagaac accggctccg cggcattcaa cagcgcacgc acgatgcgat cggccatgaa
     3721 atacgtgccc agttcggtgc gcgccatctg cagcgaagcg gccgccggca gcgcgaacga
     3781 ataggcggcg ccggcgatcg ccaccggcat catggtccag cccaggcgca gcccggcgcg
     3841 cgcctcggcg cgggcccaca gggccgaggt gcgcgccccg gcgaagtcgc gccgcagcca
     3901 gc
//
LOCUS       DASRDG010000496         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1585877, whole genome shotgun sequence.
ACCESSION   DASRDG010000496 DASRDG010000000
VERSION     DASRDG010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585877"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFA35_08350"
     CDS             <1..721
                     /locus_tag="VFA35_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT56221.1"
                     /translation="RPAPVIEALRGVSFTLRAGRSLGVVGESGSGKSTLARLVMALEA
                     PTEGRVALLGRDLHTLAPSALRAARRDFQMVFQDPYGSLDPHQRVGRIVAEPLVGGDA
                     AATRRRVAEALDAVGLRATDADKYPHEFSGGQRQRIAIARALITRPQLIVADEPVSAL
                     DVSVQAQVLNLLAELQQQFGVTYLFISHDLAVIDQVCDDVAVMRAGLIVEHGPTEQVL
                     QRPAHAYTQALLAAMPEALPR"
     gene            819..>2027
                     /locus_tag="VFA35_08355"
     CDS             819..>2027
                     /locus_tag="VFA35_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT56222.1"
                     /translation="MSPNPRPAALALIALVAAAATFAPPLAQAQMRKDSAVLGMILEP
                     APGLDPTAASAAAIGEVVHYNILEGLTKINMDGSIAPLLAESWTVDPDGKSYTFKLKK
                     GVKFSDGEAFDASDVKFSFERAKAPGSTNKGKRAVFDNISRIDTPDPHTVIVVLNQPD
                     GNFLFRMGENTAVILDPKSAATTATKPVGTGPFKLDAWAKGASITLSRNEGFRNPAAI
                     KLKKVTFRFISDPAAQVAALLAGDIDGMPRFGAIQSLKQFQSDARFSVVQGGTEGKTI
                     VGINNKRKPFDDVRVRRALAAAIDRKAIIDGSSEGYGKPIGSHMVPSDAGYIDLTGAN
                     PYDPEKARALLKEAGVATPLNVTLTLPPQSYARQGGEIVAAQLATVGVNAKIENVEWA
                     QWLSGTFKGNF"
BASE COUNT          343 a          729 c          693 g          262 t
ORIGIN      
        1 ccggccggcg ccggtcatcg aggcgctgcg cggcgtgagc ttcacgctgc gagccgggcg
       61 cagcttggga gtggtcggcg aatcgggctc gggcaagtcg acgctggcgc ggctggtgat
      121 ggcgctggag gcgccgaccg aaggccgcgt cgcgctgctc ggccgcgacc tgcacacgct
      181 cgcgccgagc gcgttgcgcg cagcccggcg cgatttccag atggtgttcc aagaccccta
      241 cggctcgctc gacccgcacc agcgcgtcgg ccggatcgtc gccgagccgc tggtcggcgg
      301 cgacgcggcc gcgacgcgcc ggcgcgtcgc cgaagcgctc gacgccgtcg gcctgcgcgc
      361 caccgacgcc gacaaatacc cgcacgagtt cagcggcggc cagcgccagc gcatcgccat
      421 cgcgcgcgcg ctgatcacgc ggccgcagct gatcgtcgcc gacgagccgg tcagcgcgct
      481 cgatgtctcg gttcaggccc aggtgctgaa cctgctggcc gagctgcagc agcagttcgg
      541 cgtcacctac ctgttcatca gccacgacct cgccgtcatc gaccaggtgt gcgacgacgt
      601 ggcggtgatg cgcgccgggc tgatcgtcga acacgggccg accgagcaag tgctgcagcg
      661 accggcgcac gcctacacgc aggcgttgct cgccgcgatg cccgaggcgc tgccgcgttg
      721 aaagcgcggt cgccttccgc ccgcggtagc gtggccagcg ccgggtgcac cacgttccgc
      781 tagagtcgaa tccccctgtc tgcgaaagtg cgtcgacgat gagcccgaac ccccgccccg
      841 ccgccctcgc gctgatcgcc ctggtggcgg ccgctgccac gttcgcgccg ccgctggcgc
      901 aggcgcagat gcgcaaggac agcgccgtgc tcgggatgat tctcgagccc gcgccgggcc
      961 tggacccgac cgccgcctcg gccgccgcga tcggcgaggt cgtgcactac aacatcctcg
     1021 aaggcctgac caagatcaac atggacggca gcatcgcgcc gctgctcgcc gaaagctgga
     1081 ccgtcgaccc cgacggcaag agctacacct tcaagctcaa gaagggcgtc aagttcagcg
     1141 acggcgaggc cttcgacgcc agcgacgtga agttcagctt cgagcgcgcg aaggcgccgg
     1201 gctcgacgaa caagggcaag cgcgcggtgt tcgacaacat cagccgcatc gacacgcccg
     1261 acccccacac cgtgatcgtg gtgctgaacc agcccgacgg caacttcctg ttccgcatgg
     1321 gcgagaacac cgcggtgatc ctcgacccga aaagcgccgc gaccaccgcg accaagccgg
     1381 tcggcaccgg gccgttcaag ctcgatgcgt gggccaaggg cgcgtcgatc acgctgtcga
     1441 ggaacgaggg ttttcgcaac ccggcggcga tcaagctgaa gaaggtcacg ttccgcttca
     1501 tcagcgaccc ggccgcgcag gtcgcggcgc tgctcgccgg cgacatcgac ggcatgccgc
     1561 gcttcggcgc gatccagagc ctgaagcagt tccaaagcga cgcgcgcttc agcgtcgtgc
     1621 aaggcggcac cgagggcaag acgatcgtcg gcatcaacaa caagcgcaag cccttcgacg
     1681 acgtgcgcgt gcgccgcgcg ctcgccgcgg cgatcgaccg caaggcgatc atcgacggct
     1741 cctccgaggg ctacggcaaa ccgatcggca gccacatggt gccgagcgac gccggctaca
     1801 tcgacctgac cggcgcgaac ccctacgacc cggagaaggc cagggcgctg ctcaaggaag
     1861 cgggcgtggc cacgccgctg aacgtgacgc tgacgctgcc gccgcagagc tacgcgcgcc
     1921 agggcggcga gatcgtcgcg gcgcagctgg ccacggttgg cgtcaatgcg aagatcgaga
     1981 acgtcgagtg ggcgcagtgg ctctcgggca cgttcaaggg caacttc
//
LOCUS       DASRDG010000497         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_783102, whole genome shotgun sequence.
ACCESSION   DASRDG010000497 DASRDG010000000
VERSION     DASRDG010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783102"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /locus_tag="VFA35_08360"
     CDS             complement(<1..1958)
                     /locus_tag="VFA35_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZT56223.1"
                     /translation="MHHLSGPCLVLAGAGSGKTRVITHKIARLLQAGYAPGQIAAITF
                     TNKAAQEMRERAKSLVGARAAKHLAISTFHSLGVRILRSEYEKLGLKQQFSILDSDDV
                     LGILKEAGASTDNALARRWQWTISAWKSAGQHSGTVEPADADERVAQRVMQRYEERLA
                     AYQAVDFDDLLALPHKLLQRDAEVRAKWQDTLRYVLVDEYQDTNAIQYEMLKSLAGER
                     ALFTAVGDDDQSIYGWRGATIANLQRLPQDYPKLKVIALEQNYRSTGNILRAANSVIG
                     NNPKLFEKKLWSAFGDGEPVRVIECDNEEHEAERVVARFQALQGGTVASASSASPTGA
                     SAVEFKDFAVLYRANHQARVFEKALRKANIPYKVSGGQSFFDRAEIKDLCAWLRLIVN
                     NDDDPAFLRAVTTPKRGIGHQTLTTLGEFAGRWKTSLYEALFAESLASVLSGRALGSL
                     HEFGRCVNDLEYRARQTSGAEDAKALLTGWLKDLDYEKHLFEGEDNEKLAAARWSNVL
                     DFVDWIAKRCGGELENDGGIAIESEKKSVLEVAQTISVIISLAERGDEQNVVTLSTLH
                     AAKGLEWPHVMLAGINEGLLPFRSEDDDMTAERLEEERRLMYVGITRARSTLSVSTLK
                     RRKRGRETVMGVPSRFIKEMKLDEVAVK"
     gene            complement(2119..>2416)
                     /locus_tag="VFA35_08365"
     CDS             complement(2119..>2416)
                     /locus_tag="VFA35_08365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZT56224.1"
                     /translation="LITRIKPGAVRIERADNGEEEFVFVAGGILEVQPGTVTVLADTA
                     IRGHDLDEAKANEAKRLAEDAMKNAKSDIDFAKAQGEFAAMAAQIAAIRKFRGR"
BASE COUNT          323 a          832 c          802 g          459 t
ORIGIN      
        1 tccttcaccg cgacctcgtc gagcttcatc tccttgatga agcggctggg cacgcccatc
       61 acggtctcgc ggccgcgctt gcggcgcttc aacgtgctga cagacagggt gctgcgcgca
      121 cgcgtgatgc cgacgtacat cagccggcgc tcctcctcga gccgctcggc ggtcatgtcg
      181 tcgtcttcgc tgcggaaggg cagcagccct tcgttgatgc cggcgagcat cacgtgcggc
      241 cactccaacc ccttggccgc gtgcagcgtc gacagcgtga cgacgttctg ttcgtcgccg
      301 cgttcggcca ggctgatgat caccgagatc gtctgcgcga cctcgagcac gctcttcttt
      361 tcgctctcga tcgcgatgcc gccgtcgttc tccagttcac caccgcaacg tttcgcgatc
      421 cagtcgacga agtcgagcac gttgctccag cgcgcggcgg cgagcttctc gttgtcttcg
      481 ccctcgaaca ggtgcttttc gtagtcgagg tccttcagcc agcccgtcag caaggccttc
      541 gcgtcctcgg cgccgctcgt ctggcgcgcg cgatattcga ggtcattcac acagcggccg
      601 aactcgtgca acgaaccaag cgcgcgaccg ctcagcaccg aggcgaggct ctcggcgaac
      661 agggcctcgt acaggctcgt cttccagcgg ccggcgaact cgccgagcgt cgtgagagtc
      721 tggtggccga tgccgcgctt gggcgtggtc accgcgcgca ggaatgccgg gtcgtcgtcg
      781 ttgttgacga tcaggcgcag ccacgcacac aggtccttga tctcggcgcg gtcgaagaag
      841 ctctggccgc ccgagacctt gtacgggatg ttcgccttgc gcagcgcctt ctcgaacacg
      901 cgcgcctggt ggttcgcgcg gtagagcacc gcgaagtcct tgaactcgac cgcgctcgcg
      961 ccggtcggcg acgcggacga cgcgctggcg accgtcccgc cctgcagggc ctgaaaccgc
     1021 gcgacgaccc gctcggcctc gtgctcctcg ttgtcgcact cgatcacgcg caccggctcg
     1081 ccgtcgccga acgcgctcca gagcttcttc tcgaacagct tcgggttgtt gccgatcacg
     1141 ctgttcgcgg cgcgcaggat gttgccggtc gagcggtagt tctgctccag cgcgatgacc
     1201 ttgagcttgg ggtagtcctg cggcaggcgc tgcaggttgg cgatggtcgc gccgcgccag
     1261 ccgtagatgc tctggtcgtc gtcgccgacc gcggtgaaca gggcgcgctc acccgcgagc
     1321 gacttcagca tctcgtactg gatcgcgttg gtgtcctggt attcgtcgac gagcacgtag
     1381 cgcaaggtgt cctgccactt cgcgcggacc tcggcgtcgc gctgcagcag cttgtgcggc
     1441 agcgcgagca ggtcgtcgaa gtcgaccgcc tggtaggccg cgaggcgctc ttcgtagcgc
     1501 tgcatcacgc gctgcgcgac gcgctcgtcg gcatcggccg gctcgaccgt gccgctgtgt
     1561 tggccggcgc tcttccaggc gctgatcgtc cactgccagc ggcgcgcgag cgcgttgtcg
     1621 gtgctcgcgc cggcctcctt caagatgccg agcacgtcgt cgctgtcgag gatgctgaac
     1681 tgctgcttca gccccagttt ctcgtattcg ctgcgcagga tgcgcacgcc cagcgagtga
     1741 aatgtgctga tggccaggtg cttggccgcg cgcgcgccga ccagcgactt cgcgcgctcg
     1801 cgcatctctt gcgccgcctt gttcgtgaac gtgatcgcgg caatctggcc cggtgcgtag
     1861 ccggcttgga gcagccgcgc gatcttgtgc gtgatcacgc gcgtcttgcc cgagcccgca
     1921 cccgcgagca cgaggcaggg gccactcagg tgatgcaccg cttcgagctg ggcaggattg
     1981 aggggatcgg acgctgcagg cacggcgcgg ctacgggggc tcgtggcatg aaaagagccg
     2041 cccggtcgcc cgggcggctc gagtcgtcgg ttcagtgcgc gacgccgcgg cggtgccggc
     2101 gccgctcaac ggcgccgctt acctgccgcg aaatttgcgg atcgccgcga tctgcgccgc
     2161 catcgccgcg aactcgcctt gcgccttcgc aaagtcgatg tcgctcttgg cgttcttcat
     2221 cgcgtcctcg gcgaggcgct tggcttcatt ggccttggct tcgtcgaggt cgtgaccgcg
     2281 gatcgcggtg tcggccagca cggtgaccgt gcccggctgc acttcgagga tgccgccggc
     2341 gacgaagacg aactcttcct cgccgttgtc ggcgcgctcg atgcgcaccg caccgggctt
     2401 gatgcgcgtg atcagc
//
LOCUS       DASRDG010000498         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1586784, whole genome shotgun sequence.
ACCESSION   DASRDG010000498 DASRDG010000000
VERSION     DASRDG010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586784"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..236
                     /locus_tag="VFA35_08370"
     CDS             9..236
                     /locus_tag="VFA35_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZT56225.1"
                     /translation="MVIGFVAGLIAKMLTPGSGPGGFFLTAALGIGGSLAATYMGRFA
                     GLYNAGQSAGFIGAVIGAVVLLLGYHLVAKR"
     gene            270..818
                     /locus_tag="VFA35_08375"
     CDS             270..818
                     /locus_tag="VFA35_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56226.1"
                     /translation="MAFDLGSLLQQYLGGAAAPNQQQAEAHFDQAAQNAPTDVLSSAL
                     SAMFRSDQTPAFGQMAGQLFGQANPNQQAGMLNSLIASMGPAVLASLLNKGGAGGGGA
                     LGSLLGQLTGSGAAPQLTPAQASQVSPEQVQVIADAAEKSNPSIVDQMSAFYAEHPTL
                     VKTVGGAALSIALAKLAEHARA"
     gene            complement(911..986)
                     /locus_tag="VFA35_08380"
     tRNA            complement(911..986)
                     /locus_tag="VFA35_08380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(951..953),aa:Arg,seq:ccg)
     gene            1149..2030
                     /locus_tag="VFA35_08385"
     CDS             1149..2030
                     /locus_tag="VFA35_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZT56227.1"
                     /translation="MSDAHHPHPHDGSHDGPHEGPVKTPKQLILAVVYAFVVPIIVIV
                     LLVEYVTSEHRPAAGTEALQPAAVAQRIRPVGIVQVKDASDLSTLKTGEQVFAAQCTT
                     CHTPGLVGAPKFGDAAAWAPRIKTGFEALLHSALAGKGSMGAQGGGDFTDLEIGRAVV
                     YMANQGGAKFAEPKPPAAGASAAVTTAAGPATPAPAAAPVAVAAAEAPKADAAGAVPA
                     LYTQLCQTCHAAGVAGAPKVGDKAAWAARLAEAKDIDGVTAVAIKGKGAMPPKGGSTA
                     SDAEIKAVVTYMVNASK"
BASE COUNT          333 a          794 c          756 g          288 t
ORIGIN      
        1 tctggaccat cgtgatcggc ttcgtcgccg gcctcatcgc gaagatgctg acgccgggct
       61 cgggcccggg cggcttcttc ctgaccgccg cactcggcat cggcggctcg ctcgccgcca
      121 cctacatggg ccgcttcgcc ggcctgtaca acgccggcca atcggccggc ttcatcggcg
      181 cggtcatcgg cgccgtcgtg ttgctgctgg gctatcacct ggtcgcgaag agatagatcg
      241 actcactttc tcaccgcaag gaaaccacca tggcattcga cctcggcagc ctgctgcagc
      301 aatacctcgg cggcgccgcc gcgccgaacc agcaacaggc cgaagcccac ttcgaccagg
      361 ccgcgcagaa cgcgccgacc gacgtgctga gcagcgcgct gtcggcgatg ttccgctccg
      421 accagacacc ggcgttcggc cagatggcgg ggcagctgtt cggccaggcg aacccgaacc
      481 agcaggccgg catgctcaac tcgctgatcg ccagcatggg accggcggtg ctggcatcgc
      541 tgttgaacaa gggcggtgcg ggtggcggtg gcgcgctcgg cagcctgctc ggccagctca
      601 ccggcagcgg cgcagcgccg cagctgaccc ccgcgcaagc ctcgcaagtc tcgcccgagc
      661 aggtacaggt gatcgccgac gcggcggaga agtcgaaccc cagcatcgtc gaccagatga
      721 gcgccttcta cgccgagcac ccgaccctgg tgaagaccgt cggcggcgcc gcgctgagca
      781 tcgcgctggc gaagctggcc gaacacgcgc gggcttgatg gcgcgcggcg cgctcggcgc
      841 gcccaagacg acggccgcat cgtgcggccg ttcgtgcttc ttgcgtcgtg aacccgactg
      901 gctgcgacgc tggtgcgccc ggctgggatc gaaccagcaa cccctgcctt cggagggcag
      961 tactctatcc attgagctac ggacgcagcg ggcgcgattc tatcagcgcg ccccgcgcga
     1021 ccccgccggc ccacgcgggt gggcgtgggc gccggcccta taattccgag gttttgcgcg
     1081 ggcccgttcg gggcccgggg gcccgccgcc ctgcccgcgc caccgacttg cgcagttcga
     1141 ggatctcaat gagcgacgcc caccacccgc acccccacga tggctcgcac gacggcccgc
     1201 acgaagggcc cgtcaagacg cccaagcaac tgatcctcgc ggtcgtgtat gcgttcgtgg
     1261 tgccgatcat cgtgatcgtg ttgctggtcg agtacgtgac cagcgaacac cgcccggcgg
     1321 cgggcaccga ggcgctgcag ccggcggcgg tggcgcagcg catccggccg gtgggcatag
     1381 tccaggtcaa ggacgcctcc gacctgtcga cgctgaagac cggcgagcaa gtgttcgccg
     1441 cgcagtgcac gacctgccac acgccgggcc tggtcggcgc gcccaagttc ggcgacgcgg
     1501 ccgcctgggc gccgcgcatc aagaccggct tcgaggcgct gctgcactcg gcgctcgccg
     1561 gcaagggcag catgggcgcg caaggcggtg gtgacttcac cgacctcgaa atcggccgcg
     1621 cggtcgtcta catggcgaac cagggcggcg ccaagttcgc cgagccgaag ccgccggcgg
     1681 ccggcgcatc ggccgcggtg acgaccgctg cagggcctgc gacgccggcg cctgccgcgg
     1741 ccccggtcgc cgtcgctgcg gccgaggccc ccaaggccga cgccgccggc gccgtgcccg
     1801 cgctgtacac ccagctgtgc cagacctgcc acgccgccgg cgtcgcgggc gcgccgaagg
     1861 tcggcgacaa ggccgcctgg gccgcacgcc tggccgaagc gaaagacatc gacggcgtga
     1921 ccgcggtcgc gatcaagggc aagggtgcga tgccgccgaa gggcggctcg accgcgtcgg
     1981 acgccgagat caaggcggtc gtgacctaca tggtgaacgc ctcgaagtag cgctgcggcg
     2041 ccccgaagaa aggccggctc gcgagccggc ttttttgcgt cctcgcaccg gcatcgtcag
     2101 cccggctgcg gcttcaggac gaagccgcag cggcccggca agtcggcgaa gcgcgtgtcg
     2161 gcaggcaccc a
//
LOCUS       DASRDG010000499         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_306586, whole genome shotgun sequence.
ACCESSION   DASRDG010000499 DASRDG010000000
VERSION     DASRDG010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306586"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFA35_08390"
     CDS             complement(<1..613)
                     /locus_tag="VFA35_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZT56228.1"
                     /translation="MTSSTRRSFLSSIAAAGAALPVARAAVAAARPTFSAPRALKILI
                     LGGTSFLGPQFVRAALAKGHTVSLFNRGKTNSKLFPDLEKLVGDRDTGDLKALEGRSF
                     DAVVDTSGYVPAHVAATAKLFADKCRHYTFISSISVFGSLGERAETLDESSPVATVSD
                     DDAAKVNTIRQSMSFYGPLKARCEAAAEAAMPGRVANIRPGLIV"
     gene            790..>2029
                     /locus_tag="VFA35_08395"
     CDS             790..>2029
                     /locus_tag="VFA35_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56229.1"
                     /translation="MVACAAPSRLAAQDKQDKQDKAVWQGFAQAQAPTWSAADLEFFL
                     HGSMGTEVVPECVFAAFRALYPDLLPGDGFAAFGFVAGARSGDLPVGVSRREVKHLGS
                     VPSLGLNCAACHTTELQSEGASLRLLGSVGHLDSEAFFGAVTAAGLRTADPANMASFL
                     REMLRAADPAQAGAALPRLDRLLAQQKDAIARAIAADPSGGKGLAFGALRELQAADLA
                     LDGARVEAGVDLAALAGAYLRLFHNMRALLHIPDQLPEKLPLTSGPGRNDAFGFLAAV
                     LFRAPTEYAPVKFGLLWNTADRPWVDWDANSRSPLGRNILTALALGAPLLGRRAELDL
                     ALVQRHTALTEVIRPPAWPWPVDRDKAARGERNYRQRCAACHEGPETDARLYDPKELG
                     TDPRRAKAFDSQHSGLFNSLF"
BASE COUNT          261 a          758 c          726 g          284 t
ORIGIN      
        1 cgacgatgag gcccggccgg atgttggcga cgcggcccgg catcgccgcc tcggccgccg
       61 cctcgcagcg cgccttgagc ggcccgtaga acgacatcga ctgccggatc gtgttcacct
      121 tcgccgcgtc gtcgtcgctc acggtcgcga ccggcgagct ctcgtcgagc gtctcggcgc
      181 gctcgccgag gctgccgaac accgagatcg agctgatgaa cgtgtagtgc ctgcacttgt
      241 cggcgaacag cttcgccgtc gctgcgacgt gcgcgggcac gtagcccgac gtgtccacca
      301 ccgcgtcgaa gctgcgccct tcgagcgcct tcaggtcgcc cgtgtcgcgg tcgccgacca
      361 gcttctccag gtcggggaac agcttgctgt tggtcttgcc gcggttgaac aagctcaccg
      421 tgtgcccctt cgcgagcgcc gcgcgcacga actgcgggcc gaggaacgag gtgccgccga
      481 ggatcaggat cttcagcgca cgcggcgcgg agaatgtggg gcgtgcggca gcgacggcgg
      541 cgcgcgcgac cggcagcgcc gcgcccgccg cggcgatcga cgagaggaac gaacggcgcg
      601 tggacgaggt catgcggccg acattacgcg cggcgcggcg aagctgctgg acccattctg
      661 ccgcggcagg agcgcgcgct tcgccgctcg ctgcgctcgc cacgctcgca aacagacctg
      721 cccggcacga tcatgccgcg catgcgtcca gctcccgccg ccggtcctgc cgccgccgtc
      781 ctcgttgcca tggtcgcctg cgcagcgccg tcccggctcg cggcgcagga caaacaggac
      841 aagcaggaca aggcggtctg gcagggcttc gcgcaggcgc aggcgccgac gtggagcgcc
      901 gcggatctcg agttcttcct gcacggctcg atgggcaccg aggtggtgcc cgagtgcgtc
      961 ttcgcggcgt tccgcgccct ctatccggac ctgctgccgg gcgacgggtt cgccgcgttc
     1021 ggtttcgtcg cgggcgcgcg cagcggcgac ctgccggtcg gcgtctcgcg ccgcgaggtg
     1081 aagcacctag gctcggtgcc gtcgctcggc ctcaactgcg ccgcgtgcca cacgaccgag
     1141 ctgcagagcg aaggcgcttc gctgcggctg ctcggcagcg tcggccacct cgactccgag
     1201 gcgttcttcg gcgccgtgac ggcggccggg ctgcgcaccg ccgacccggc gaacatggcg
     1261 tcgttcctgc gcgagatgct gcgcgccgcc gatcccgcgc aggctggcgc ggcgctgccg
     1321 cgcctcgacc ggttgctcgc gcagcagaag gacgcgatcg ccagagcgat cgccgccgat
     1381 ccgtcgggcg gcaagggact cgcgttcggc gcgctgcgcg agctgcaggc cgccgacctc
     1441 gcgctcgacg gcgcgcgcgt cgaggccggc gtcgatctgg cggcgctcgc gggcgcgtat
     1501 ctgcggctgt tccacaacat gcgcgccttg ctgcacatcc ccgaccagct gccggagaag
     1561 ctgccgctga cgtcggggcc ggggcgcaac gacgcgttcg gcttcctcgc ggccgtcctg
     1621 ttccgcgcgc cgaccgagta cgcgccggtg aagttcggcc tgctctggaa caccgccgac
     1681 cggccgtggg tcgactggga cgcgaactcg cgctcgccgc tcggccgcaa catcctcacc
     1741 gccctcgccc tcggcgcgcc gctgctcggc cgccgcgccg agctcgacct ggcgctcgtg
     1801 cagcgccaca cggcgctcac cgaggtgatc cggccgcccg cgtggccctg gcccgtcgac
     1861 cgcgacaagg ccgcgcgcgg cgagcgcaac tacaggcagc gttgcgcggc gtgccacgaa
     1921 gggcccgaga ccgacgcgcg gctctacgac ccgaaggagc tgggcaccga tccgcggcgg
     1981 gcgaaggcgt tcgactcgca gcactcgggg ctgttcaaca gcctgttcg
//
LOCUS       DASRDG010000500         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_189118, whole genome shotgun sequence.
ACCESSION   DASRDG010000500 DASRDG010000000
VERSION     DASRDG010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189118"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFA35_08400"
     CDS             <1..287
                     /locus_tag="VFA35_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZT56230.1"
                     /translation="AAGLTTVKKDELQPGDLVFFNTLRRTFSHVGIYIGDGKFVHSPK
                     PGGEVRVEDMRIVYWAKRFTGARRAEPMADPTQAPGPSLTQTLAPAATLR"
     gene            347..1459
                     /gene="moaA"
                     /locus_tag="VFA35_08405"
     CDS             347..1459
                     /gene="moaA"
                     /locus_tag="VFA35_08405"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZT56231.1"
                     /translation="MADKVIPLADLRYAASVPRVPLLDTPPTGLLGDTLGRPLRDLRI
                     SVTDRCNFRCSYCMPKEVFDKQYAFLPHSSLLSFEEITRTARLFVAHGVRKIRLTGGE
                     PLLRKNLEVLIGMLSALRTPDGAPLDLTLTTNGSVLARKAQALKDAGLQRVTVSLDAL
                     DDTVFRRMNDADFPVAEVLAGIAEARRVGLGPIKVNMVVKRGTNDDQILPMARHFKGT
                     GAVLRFIEYMDVGATNGWRMDEVLPSAQVVERIHAELPLQAIAPNVLGETAERWRYAD
                     GSGEVGVISSVTQAFCQDCNRARLSTEGKLFLCLFASHGHDLRALLRGGYTDAQIAGA
                     IGLIWQGRDDRYSELRANAVAAPGGERRVEMHYIGG"
     gene            1459..1971
                     /locus_tag="VFA35_08410"
     CDS             1459..1971
                     /locus_tag="VFA35_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT56232.1"
                     /translation="MAAVAIRRLVPANLPAYKSLRDAVLTAHPEAFTSDPAPERPPES
                     YLPRLGLDRAEGGVFTLGAWEGARLVGAVSCERDPRAKVLHIGHLIGMMVRSDAQGRG
                     IGRALLEACIAEARAARGLAMLTLTVTAGNDAAVHLYERAGFTRYGRLERAICVDGVY
                     HAKDQMTLAL"
     gene            complement(1978..>2373)
                     /locus_tag="VFA35_08415"
     CDS             complement(1978..>2373)
                     /locus_tag="VFA35_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HZT56233.1"
                     /translation="ARNGLSRGANGLKVIMMCELPTNAILAREFLEHFDGMSIGSNDL
                     TQLTLGLDRDSGMELLAKDFDERDPAVKALISKAINACLAAGKYVGICGQGPSDHPDF
                     ADWLAAEGIVSISLNPDSVVETWRRLAAR"
BASE COUNT          362 a          863 c          810 g          338 t
ORIGIN      
        1 cggccgccgg gttgacgacg gtcaagaagg acgagttgca acccggcgac ctggtgttct
       61 tcaacacgct gcgccgcacc ttctcgcacg tgggcatcta catcggcgac ggcaagttcg
      121 tgcactcgcc gaaaccgggc ggcgaggtgc gggtcgaaga catgcgcatc gtctactggg
      181 cgaagcgctt caccggcgcg cgccgggccg agccgatggc cgacccgacc caggcgcccg
      241 ggccatcgct gacccagacg ctcgcgccag cggcgacgct gcgctgaacc ggcgcccgcc
      301 tggcgcgcgc ccggcagggc caagcgcgcg caggcacaat ctcgccatgg ccgacaaagt
      361 cattcccctc gccgacctcc gctacgccgc cagcgtgccg cgcgtgccgc tgctcgacac
      421 cccgccgacc ggcctgctcg gcgacacgct cggccggccg ttgcgcgacc tgcgcatctc
      481 ggtcaccgac cgctgcaact tccgctgcag ctactgcatg ccgaaggagg tgttcgacaa
      541 gcagtacgcc ttcctgccgc actcgtcgct gctgagcttc gaggagatca cccgcaccgc
      601 gcggctgttc gtcgcgcacg gcgtgcgcaa gatccgcctg accggcggcg agcccttgct
      661 gcgcaagaac ctcgaagtgt tgatcgggat gctgagcgcg ctgcgcacgc ccgacggcgc
      721 gccgctcgac ctgacgctga cgacgaacgg ctcggtgctc gcgcgcaagg cgcaggcgct
      781 gaaggacgcc gggctgcagc gcgtgacggt gagcctcgat gcgctcgacg atacggtgtt
      841 ccgccgcatg aacgacgccg acttcccggt cgccgaagtg ctcgccggca tcgcagaagc
      901 gcggcgcgtc ggcctcggcc cgatcaaggt caacatggtc gtcaagcgcg gcaccaacga
      961 cgaccagatt ctgccgatgg cgcgccactt caagggcacg ggcgcggtgc tgcgcttcat
     1021 cgaatacatg gatgtcggcg cgaccaacgg ctggcgcatg gacgaggtgc tgccctcggc
     1081 gcaggtcgtc gagcgcatcc atgccgaact gccgctgcag gcgatcgcgc ccaacgtgct
     1141 cggcgagacc gccgagcgtt ggcgctatgc cgacggcagc ggcgaggtcg gcgtgatctc
     1201 gagcgtcacg caggcgttct gtcaggactg caaccgcgcg cgcctgtcga ccgaaggcaa
     1261 gctgtttctc tgcctgttcg cgagccacgg ccacgacctg cgcgcgctgc tgcgcggcgg
     1321 ctacaccgac gcgcagatcg ccggcgcgat cggcctgatc tggcaagggc gcgacgaccg
     1381 ctactccgaa ctgcgcgcga acgccgtggc cgcgcccggc ggcgagcgcc gcgtcgagat
     1441 gcattacatc ggcggctgat ggcggcggtc gcgatccggc gcctcgtgcc ggccaatctg
     1501 cccgcgtaca agagcctgcg cgatgcggtg ctgaccgcgc accccgaagc cttcacctcg
     1561 gaccccgcac ccgaacgccc gcccgagagc tacctgcccc gcctcggcct cgaccgcgct
     1621 gaaggcggcg tgttcacgct cggtgcgtgg gagggcgcgc gcctcgtcgg cgccgtcagc
     1681 tgcgagcgcg atccgcgcgc caaggtgctg cacatcggcc acctcatcgg gatgatggtg
     1741 cgcagcgacg cgcaaggccg gggcatcggc cgcgcgctgc tcgaggcctg catcgccgag
     1801 gcgcgcgccg cgcgcggcct cgcgatgctg acgctcacgg tgacggccgg caatgacgcg
     1861 gccgtgcacc tgtacgagcg cgccggcttc acacgctatg gccggctcga acgcgccatc
     1921 tgcgtcgacg gcgtgtatca cgccaaggac cagatgacgc ttgcgctctg acgcggctca
     1981 gcgcgccgcg agacgccgcc aggtctcgac gaccgagtcc gggttcagcg agatcgagac
     2041 gatgccttcg gcggcgagcc agtccgcaaa gtccgggtgg tcgctcggcc cctgcccgca
     2101 gatgccgacg tacttgccgg ccgcgagaca cgcgttgatc gccttcgaga tcagcgcctt
     2161 cacggccggg tcgcgctcgt cgaagtcctt ggcgagcagt tccatgcccg agtcgcggtc
     2221 caggcccagg gtcagctgcg tcaggtcgtt cgagccgatc gacatgccgt cgaagtgctc
     2281 gaggaactcg cgcgcaagga tggcattcgt cggcagctcg cacatcatga tgaccttgag
     2341 gccgttcgcg ccgcgcgaga gcccgttccg ggc
//
LOCUS       DASRDG010000501         4790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1108758, whole genome shotgun sequence.
ACCESSION   DASRDG010000501 DASRDG010000000
VERSION     DASRDG010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108758"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFA35_08420"
     CDS             complement(<1..183)
                     /locus_tag="VFA35_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HZT56234.1"
                     /translation="MNRLTALALVLAGIALAGCDTMSEALNKRPPVDVLKTEAVRPQP
                     VVETRVNNGSIFQAGQY"
     gene            complement(180..962)
                     /gene="flgG"
                     /locus_tag="VFA35_08425"
     CDS             complement(180..962)
                     /gene="flgG"
                     /locus_tag="VFA35_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HZT56235.1"
                     /translation="MMRSLWISKTGMEAQQTQLDTISHNLANASTNGYKKSHAVFEDL
                     MYQNLRQAGANSTDQTTLPTGLQLGLGTRAVATSRSFAQGNLQQSSNPLDVAVSGQGF
                     FQVQMPDGTTGYTRDGAFQVNATGQLVTNNGHTVQPGITIPANATSVTIGNDGTVSVV
                     LPAQTAPQTVGKIQTANFINPAGLDPLGQNLYAETAASGTPNTGTPGQNGLGLLQQGF
                     VETSNVNVVEELVGMIQTQRAYELNSKAIKTSDDMLARLTQL"
     gene            complement(1068..1664)
                     /locus_tag="VFA35_08430"
     CDS             complement(1068..1664)
                     /locus_tag="VFA35_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZT56236.1"
                     /translation="MRFIDAAEDKPAKPDPKGDKLEEQLAFKSRFVLVFGEITDALAH
                     ATCRRLLALSEESEAPITMLISSPGGHVESGDAIFDLIRFVRAPVTTVGSGWVASAGA
                     HIFLAPPKERRLCLPNTRFMIHQPAGGAGGQATDIAIQAKEIIKARERLARVVSTQTG
                     QPYEKVAADMERDYWMSADEAIAYGIVSRTIERQKDLG"
     gene            complement(1749..2477)
                     /gene="flgF"
                     /locus_tag="VFA35_08435"
     CDS             complement(1749..2477)
                     /gene="flgF"
                     /locus_tag="VFA35_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HZT56237.1"
                     /translation="MDRMIYLSMSGAKATMQRQDTLANNLANVSTPGFRAELQAFRAV
                     PVQGSGASTRVYALETTTGYDASAGAVTATGRNLDVAVKGSSWLTVQALDGTEAHTRG
                     GSLEVSNDGTLTTRSGLPVLGDGGPLQVPPNSAVSIGADGTVSAKGADGKNTAIGKLK
                     LVTPEAPLQRGADGLFRGADGNTLNADAAARVQDGALEGSNVSAVEAMVAMISAARQF
                     EAQMKMISTAEADEKAASQLLTAN"
     gene            complement(2625..3881)
                     /gene="flgE"
                     /locus_tag="VFA35_08440"
     CDS             complement(2625..3881)
                     /gene="flgE"
                     /locus_tag="VFA35_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HZT56238.1"
                     /translation="MSFQQGLSGLNASSKNLEVIGNNVANANTIGAKASRAEFGDMYA
                     SAINGTSIGIGVNLQAVTQQFTQGNITTTDNPLDLAINGNGFFEVQNPGGQTLYTRNG
                     QFQADKDGFITTNAGLKLVGYPATPDGTIQPGAARPLQLPTGGVAPQPTSAITVEMNL
                     DARKATTLPAGTPQIDFTNAKTYNDATSVTVFDAKGQDVALTYYFQKSATDTWNVYAT
                     ANGSSVKVDGAGNPLPITQIDFPPNGGTPTAPTGTVPFDVPSTTNTAGAATLPITGIA
                     LDLRGATQFGSGFGVTNMTQDGYAAGLLTGVTVEKSGIVMATYSNGQSKPAGQLELAT
                     FRNPQGLSPLGGNLWARSYASGDPTVGTPSTGNIGALQAGALEESNTDLTGELVDMIT
                     AQRVYQANAQTIKTQDQVLQTLVNLR"
     gene            complement(3903..4547)
                     /locus_tag="VFA35_08445"
     CDS             complement(3903..4547)
                     /locus_tag="VFA35_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HZT56239.1"
                     /translation="MDISAATLSNINGTTAAASKVDDPGSQDRFLKLLVTQLQNQDPL
                     SPMDNAQLTSQIAQINTVTGIATLNTSVQGLSSQFLQMQTLQGASLVGKSVIVPGNKV
                     DIADGVGQGGFELATPADAVKVEVLSAAGQVVDTLNLGAQSAGLHGFDWVAGQYDNTS
                     NLTFRVSATSGATRLDATALMRDTVNAVSASGSALQLQLARAGTVDYGTVKAIN"
     gene            complement(4577..>4790)
                     /locus_tag="VFA35_08450"
     CDS             complement(4577..>4790)
                     /locus_tag="VFA35_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HZT56240.1"
                     /translation="SGIYESQAPALQRYEPGNPLADTNGYVYAPSVNVVEEMVNMISA
                     SRSYQNNIEVMNTAKSLLLKTLQLGS"
BASE COUNT          590 a         1557 c         1727 g          916 t
ORIGIN      
        1 gtactggccg gcctggaaga tcgaaccgtt gttgaccctt gtttcgacga ccggctgcgg
       61 gcgcaccgcc tcggtcttca gcacgtcgac cggcgggcgc ttgttcagcg cctcgctcat
      121 cgtgtcgcag cccgcgaggg cgatgccggc gagcaccagc gccagggcgg tcagccggtt
      181 catagctgcg tcagtcgggc gagcatgtcg tccgaggtct tgatcgcctt cgagttcagc
      241 tcgtaggcgc gctgggtctg gatcatgccg acgagttctt cgacgacatt gacgttcgag
      301 gtctcgacga agccctgctg cagaagcccc aaaccgttct gccccggcgt gccggtgttc
      361 ggcgtgcccg acgccgcggt ctcggcgtag aggttctggc ccagcgggtc gaggccggcc
      421 gggttgatga agttcgcggt ctggatcttg ccgaccgtct gcggcgcggt ctgcgccggc
      481 agcacgacgc tgaccgtgcc gtcgttgccg atcgtcacgc tggtcgcatt cgcgggaatc
      541 gtgatgccgg gctgcacggt gtggccgttg ttcgtgacca gctgaccggt cgcgttgacc
      601 tggaacgcgc cgtcgcgcgt gtagccggtc gtgccgtcgg gcatctgcac ctgaaagaaa
      661 ccctggccgc tgacggccac atcgagcggg ttgctgcttt gctgcaggtt gccctgcgcg
      721 aagctgcgcg aggtcgcgac cgcgcgtgtg cccaggccga gctgcaggcc ggtcggcaac
      781 gtcgtctggt cggtgctgtt ggcgccggcc tggcgcaggt tctggtacat caggtcctcg
      841 aacaccgcgt gcgacttctt gtagccgttg gtcgaggcgt tggcgaggtt gtgcgagatg
      901 gtgtccagct gggtctgctg ggcttccatg ccggtcttcg agatccacag ggaacgcatc
      961 atggcggggc tccggtcaat ggggccgatg atgaagcggc cccgctgccg aagtggccgg
     1021 ataagcgggc ggatcgccgc cctgttctgc gcccgggccg gctcgggtca gccgaggtcc
     1081 ttctgccgct cgatcgtgcg cgagacgatg ccgtaggcga tcgcctcgtc ggcgctcatc
     1141 cagtagtcgc gctccatgtc ggccgcgacc ttctcgtagg gctggccggt ctgggtcgag
     1201 accacgcgcg cgaggcgctc gcgggccttg atgatctcct tggcctggat cgcgatgtcg
     1261 gtcgcctggc cgcccgcgcc gccggccggc tggtggatca tgaagcgggt gttgggcagg
     1321 cacaggcggc gctctttcgg cggcgcgagg aagatgtgcg cgccggcgct ggcgacccag
     1381 ccgctgccca ccgtcgtgac cggcgcgcgc acgaagcgga tcaggtcgaa gatcgcatcg
     1441 cccgactcga catgcccgcc cggcgacgag atcagcatcg tgatcggcgc ctcggattcc
     1501 tccgagagcg cgagcaggcg gcggcaggtc gcgtgcgcga gcgcgtcggt gatctcgccg
     1561 aacacgagca cgaagcgcga cttgaacgcg agctgctctt cgagcttgtc gcccttgggg
     1621 tccggcttcg cgggcttgtc ttcggcggcg tcgatgaatc tcatggcttg ctcctgaatg
     1681 ggtctgtgcg gacgagttta agccgcccgc atcggcaccc ggaccaggag ggttgcgccg
     1741 gcgcgccgtc agttcgcggt gagcaactgc gacgccgcct tctcgtcggc ctcggcggtc
     1801 gagatcatct tcatctgcgc ctcgaactgg cgcgccgccg agatcatcgc gaccatggct
     1861 tcgaccgcgc tgacgttcga gccttcgagc gcgccgtcct gcacgcgcgc ggcggcatcg
     1921 gcgttcagcg tgttgccgtc ggcgccgcgg aacaggccgt cggcgccgcg ctgcagcggc
     1981 gcttcgggcg tgacgagctt gagcttgccg atcgcggtgt tcttgccgtc ggcgcccttc
     2041 gcgctgaccg tgccgtcggc gccgatgctc acggccgagt tcggcggcac ctgcagtggg
     2101 cccccgtcgc ccagcaccgg caggccgctg cgggtcgtca gcgtgccgtc gttcgagact
     2161 tcgagcgagc cgccgcgggt gtgggcctcg gtgccgtcga gcgcctgcac cgtgagccag
     2221 ctgctgccct tgacggcgac atcgaggttg cggccggtcg cggtgaccgc gccggcactg
     2281 gcgtcgtagc cggtcgtggt ctccagcgcg tagacccgcg tgctcgcgcc gctgccctgc
     2341 accggcaccg cgcgaaatgc ctgcagctcg gcgcgaaagc ccggcgtcga cacgttcgcg
     2401 aggttgttcg cgagcgtgtc ctggcgctgc atcgtcgcct tcgcgcccga catcgacagg
     2461 tagatcatgc ggtccatatg actactcccc gctcgacgag tacgtcgagc gaccctccat
     2521 gggccacgaa ggggcccctt cgcggccctg ggtcgggcct tgcttggggc ggcccggcgc
     2581 ggcggcccgt tgcgctttga gtgacatcgg atcagtgcga cgcatcagcg caggttgacg
     2641 agcgtttgaa gcacctggtc ctgcgtcttg atcgtctgcg cattcgcctg gtagacgcgc
     2701 tgcgcggtga tcatgtcgac gagctcgccg gtcaggtcgg tgttcgactc ttcgagcgcg
     2761 ccggcctgca gcgcgccgat gttgccggtg ctcggcgtgc cgaccgtcgg gtcgcccgat
     2821 gcatagctgc gcgcccacag gttgccgccc agcggcgaga ggccctgcgg gttgcggaag
     2881 gtcgcgagtt cgagctggcc ggccggcttg gactggccgt tcgagtaggt cgccatcacg
     2941 atgccgctct tctcgaccgt cacgccggtc agcaagcccg cggcgtagcc gtcctgcgtc
     3001 atgttggtca cgccgaagcc ggagccgaac tgggtcgcac cgcgcaggtc gagcgcgatg
     3061 ccggtgatcg gcagcgtggc cgcgccggcg gtgttcgtgg tcgagggcac gtcgaacggc
     3121 acggtgcccg tcggcgcggt cggcgtgccg ccgttgggcg ggaagtcgat ctgcgtgatc
     3181 ggcagcgggt tgcccgcgcc gtcgaccttg accgagctgc cgttcgcggt cgcatagacg
     3241 ttccaggtgt cggtggccga cttctggaag tagtaggtca gcgccacgtc ctggcccttg
     3301 gcgtcgaaca cggtcaccga ggtcgcgtcg ttgtaggtct tcgcgttcgt gaagtcgatc
     3361 tgcggcgtgc ccgccggcag ggtcgtcgcc ttgcgcgcat cgaggttcat ctcgaccgtg
     3421 atcgcgctcg tcggctgcgg cgccacgccg ccggtgggca gctgcagcgg ccgggccgcg
     3481 ccgggctgga tcgtgccgtc cggcgtggcc gggtagccga ccagcttcag gccggcgttg
     3541 gtcgtgatga agccgtcctt gtcggcctgg aactggccgt tgcgcgtata cagcgtctgg
     3601 ccgccggggt tctgcacctc gaagaaaccg ttgccgttga tcgccaggtc gagcgggttg
     3661 tcggtggtcg tgatgttgcc ctgggtgaac tgctgcgtca ccgcttgcag gttcacgccg
     3721 atgccgatgc tggtgccgtt gatcgcgctc gcgtacatgt cgccgaactc ggcgcgcgac
     3781 gccttggcgc cgatcgtgtt ggcgttggcg acgttgttgc cgatgacctc gaggttcttg
     3841 ctcgacgcgt tcagaccgga aagaccttgc tggaagctca tgggtgttcc ttggttgctg
     3901 gatcagttga tcgccttgac cgtgccgtag tcgaccgtgc cggcgcgcgc cagctgcagc
     3961 tgcagagcgc tgcccgaggc gctgaccgcg ttgacggtgt cgcgcatcag cgcggtcgcg
     4021 tcgaggcggg tcgcgccgct ggtcgcgctg acacggaagg tgaggttgct ggtgttgtcg
     4081 tattggcccg cgacccagtc gaagccgtgc aggccggcgc tttgcgcacc gaggttgagc
     4141 gtgtcgacga cctggccggc ggcgctcagc acctcgacct tgaccgcgtc ggccggcgtc
     4201 gcgagctcga agccgccctg gccgacgccg tcggcgatat cgaccttgtt gcccggcacg
     4261 atcacgctct tgccgaccag cgacgcgccc tgcagcgtct gcatctgcag gaactggctc
     4321 gacaggccct gcacgctggt gttcagcgtg gcgatgccgg tcaccgtgtt gatctgcgcg
     4381 atctggctcg tcagctgcgc gttgtccatc gggctcagcg gatcctggtt ctgcagctgg
     4441 gtgacgagca gcttcaggaa gcggtcctgg ctgcccgggt cgtcgacctt gctggcggcg
     4501 gcggtcgtgc cgttgatgtt gctgagcgtg gcggcggaga tgtccatgcg cgtttccctc
     4561 ggggtcggcg gcgcgctcac gagccgagtt gcaaggtctt gagcagcagg ctcttcgccg
     4621 tgttcatgac ttcgatgttg ttctggtacg aacgcgaggc cgagatcatg ttgaccatct
     4681 cctcgaccac gttcaccgac ggcgcgtaca cgtagccgtt ggtgtcggcc agcggattgc
     4741 ccggttcgta gcgctgcagg gccggcgcct gtgattcgta gatgccgctg
//
LOCUS       DASRDG010000502         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_530837, whole genome shotgun sequence.
ACCESSION   DASRDG010000502 DASRDG010000000
VERSION     DASRDG010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530837"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFA35_08455"
     CDS             complement(<1..256)
                     /locus_tag="VFA35_08455"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HZT56241.1"
                     /translation="MLHFSHAFAAIDSHTAGEPTRIVTGGLPKVAGATLAEKRTTLQR
                     EHDWLRRALVLEPRGHDAIVIALLLPPCDPQAHLGVVFAND"
     gene            complement(337..1329)
                     /locus_tag="VFA35_08460"
     CDS             complement(337..1329)
                     /locus_tag="VFA35_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZT56242.1"
                     /translation="MTEPIPSIVRGARQAPVPVGPLTGRRTALRRAGAHALAAVNRLL
                     DRLPVGRTLHRRIQHALATSELPFACARGGAGLDGLRIAFLTDLHAGSFMDASDLCRV
                     MERVAAAEPHLVLFGGDLINTREREILLYRDALRLLRPELGVFAVPGNHDHFWGHDIG
                     LWTAFLREHGVQVLLNRGVRIERRGASLWLCGVDDLTEGAPDLRAALDGRSADEPALL
                     LSHHPDFFFEAAAAGVDLTLSGHTHGGQIRIGGWAPIHHSRFGYERGWFAEGGSRLYV
                     SRGIGVTVLPLRIGAPPEIPVVVFAVQQANAAARSDAAAANARGGATAAAAAKA"
     gene            complement(1326..>2427)
                     /locus_tag="VFA35_08465"
     CDS             complement(1326..>2427)
                     /locus_tag="VFA35_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZT56243.1"
                     /translation="ADDILEKPVALPELELCCARLRSRRRLLRENRFFRAESVGGELL
                     VAGPRTRAVVALVERVAASKATVLVQGESGTGKERVAALLHRRSDRRDGPFVKLNCAS
                     LAESLLESELFGHEQGAFTGANRRREGRFELADGGTLFLDEVGEMSPAMQSKLLRVLQ
                     EGELQRLGGNRTIAVDVRVVAATNRDLEREVAAGRFRQDLYYRLAVVPIVLPPLRQRP
                     EEIVPLARHFLPAGLSLEPAAEQALLAWHWPGNVRELQNVVQRASLLGDGEVVRADLL
                     AGWLRPQALAAGAAEPVYVLPAAAPADAVAVLVGRSLGDVEAELIQRTLQHCGGNRTR
                     TAELLGIGVRTLFNKLRPPGHPTVNPIGQPTG"
BASE COUNT          311 a          917 c          905 g          294 t
ORIGIN      
        1 cgtcgttcgc gaacacgacg ccgaggtgcg cctgcggatc gcacggcggc agcagcagcg
       61 cgatgacgat cgcgtcgtgg ccgcgcggct cgagcaccag cgcgcgccgc agccagtcgt
      121 gctcgcgctg cagcgtcgtg cgcttctccg ccagcgtcgc gccggcgacc ttcggcaggc
      181 cgccggtgac gatgcgcgtc ggttcgccgg cggtgtgcga atcgatcgcc gcgaacgcgt
      241 gcgagaagtg cagcatcgcc gcatcatgcc gcgggcgtcc gcgacgtgcg cgcgccgtcc
      301 gtccaggtcg cggaccggcc gcggaatccg cctcgatcac gccttcgctg ctgcggcggc
      361 cgtggcgccg ccgcgtgcgt tggccgccgc cgcgtcgctg cgcgcggccg cgttcgcctg
      421 ctgcaccgcg aacaccacca ccgggatctc cggcggcgcg ccgatgcgca gcggcagcac
      481 cgtgacgccg atgccgcggc tgacgtagag gcggctgccg ccttcggcga accagccgcg
      541 ctcgtagccg aagcggctgt ggtggatcgg cgcccagccg ccgatccgga tctggccgcc
      601 gtgcgtgtgc cccgacagcg tgaggtcgac gcctgctgcc gccgcctcga agaagaagtc
      661 gggatggtgc gagagcagca gcgccggttc gtcggcagat cggccgtcga gcgccgcgcg
      721 cagatcgggc gcgccctcgg tgaggtcgtc gacgccgcac agccacagcg acgcgccgcg
      781 ccgctcgatg cgcacgccgc ggttgagcag cacctgcacg ccgtgctcgc gcaggaacgc
      841 ggtccacagg ccgatgtcgt ggccccagaa gtggtcgtgg ttgcccggca ccgcgaacac
      901 gccgagctcc ggccgcagga ggcgcagcgc atcgcggtag agcaggatct cgcgttcgcg
      961 cgtgttgatg aggtcgccgc cgaacagcac gaggtgcggc tcggccgccg ccacgcgctc
     1021 catcacgcgg cacaggtcgg acgcgtccat gaagctgcct gcgtgcaggt cggtcaggaa
     1081 cgcgatccgg aggccgtcga ggcccgcgcc gccgcgcgcg cacgcgaacg gcagctcgct
     1141 cgtggcgagc gcgtgctgga tgcggcggtg cagcgtgcgc ccgaccggca gccggtcgag
     1201 cagccggttc accgccgcga gcgcgtgcgc acccgcgcgc cgcagcgcgg tgcggcgccc
     1261 ggtcagcgga cccacgggca ccggtgcctg gcgggcgccg cggacgatgg acggaatcgg
     1321 ttcggtcatc cggtcggctg cccaatcggg ttcacagtcg ggtgccccgg cgggcgcagc
     1381 ttgttgaaca gtgtacgcac gccgatgccc agaagctccg ccgtccgcgt gcggttgccg
     1441 ccgcagtgct gcagtgtgcg ctggatcagc tccgcctcga cgtcgccgag cgaacggccg
     1501 accagcaccg cgaccgcgtc cgcgggcgcc gcggcgggca gcacgtagac gggctccgcg
     1561 gcgccggccg ccagcgcctg cggccgcagc cagcccgcga gcaggtcggc gcgcacgacc
     1621 tcgccgtcgc cgagcagcga cgcgcgctgc acgacgttct gcagctcgcg cacgttgccg
     1681 ggccagtgcc acgcgagcag cgcctgttcc gccgcgggct cgagcgacag cccggcgggc
     1741 aggaagtggc gcgcgagcgg cacgatctcc tcgggccgct gccgcagcgg cggcagcacg
     1801 atcggcacca ccgcgagccg gtagtagagg tcctggcgga agcggccggc ggcgacctcg
     1861 cgctcgaggt cgcggttggt cgccgcgacc acgcgcacgt cgacggcgat cgtgcggttg
     1921 ccgcccagcc gctgcagctc gccctcctgc agcacgcgca acagcttgct ctgcatcgcc
     1981 ggcgacatct cgccgacctc gtcgaggaac agcgtgccgc cgtcggcgag ctcgaagcgg
     2041 ccctcgcggc gccggttggc gcccgtgaac gcgccctgct cgtggccgaa cagctcgctc
     2101 tcgagcagcg actcggcgag cgacgcgcag ttgagcttca cgaacggccc gtcgcgccgg
     2161 tcgctgcgcc ggtgcagcag cgcggcgacg cgctccttgc cggtgccgct ctcgccctgc
     2221 acgagcacgg tggccttgct cgccgccacg cgctcgacca gcgcgacgac ggcgcgcgtg
     2281 cgcggccccg cgaccagcag ctcgccgccg acgctctcgg cgcggaagaa gcggttctcg
     2341 cgcagcaggc gccggcggct gcgcagccgc gcgcagcaga gctcgagctc gggcagcgcg
     2401 accggcttct cgaggatgtc gtcggcg
//
LOCUS       DASRDG010000503         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1336604, whole genome shotgun sequence.
ACCESSION   DASRDG010000503 DASRDG010000000
VERSION     DASRDG010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336604"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VFA35_08470"
     CDS             complement(<1..1459)
                     /locus_tag="VFA35_08470"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HZT56244.1"
                     /translation="MFAWINRLDHYFPIRYGAWALCIVAFLASMAGWMRMGFEPWLAI
                     VFGVLVLTGLRDVIQTKHAILRNYPVIGHLRFLLEFIRPEMRQYFIEDDRDAAPFSRQ
                     QRSLVYQRAKGDSDKRPFGTQLDVNAVGYEWINHSLQPSLLKTHDFRVLVGAGRAQPY
                     SASIFNISAMSFGALSANAILALNAGAKRGNFAHDTGEGSISRHHRVNGGDLIWEIGS
                     GYFGCCNQDGSFSEEYFTANARDPQVKMIELKLSQGAKPGHGGVLPGPKVTLEIAEAR
                     HVQPWVDCVSPASHGAFRTPIEMMHFIERLRELSGGKPTGFKLCIGHVWEWFGIAKAM
                     LETGITPDFIVVDGAEGGTGAAPLEFTDHVGMPLQEGLLLVHNTLVGLDLRKKIRLGC
                     AGKVVSAFDIARMLALGADWCNAARGFMFALGCIQAQTCHTGACPTGVTTQDAQRMKA
                     LDVPTKADRVYHFHQNTMMALKELVQAAGLEDPAGI"
     gene            complement(1542..>2472)
                     /locus_tag="VFA35_08475"
     CDS             complement(1542..>2472)
                     /locus_tag="VFA35_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZT56245.1"
                     /translation="LRGLPRADSLTLDPHKGMFLPYGTGALLVRDGAALRAAHEATAD
                     YLPAMPHPSDFYDPSQHGPELSRGFPGLRVWLSLKMFGAAAFRSAIAEKRALALDAAE
                     LMSALGHERFAVAGHDRGGRVAHRLALDHPERVARLAVIDIAPTLDMYAATDMRFASR
                     YYHWFFLIQPAPLPERMIGGDPAFYLRWTLGGWGTNGIDFIEPEALAEYERCFCRADT
                     IHATCEDYRAGASIDLEHDRASRSADEKIACDLYLLWGSRGLVGKRFDPLALWRARAA
                     GRVEGAAMEAGHFIPEELPDDTARRLRDFFLAA"
BASE COUNT          374 a          819 c          907 g          372 t
ORIGIN      
        1 tgatgcccgc cgggtcctcg aggcccgccg cctgcaccag ctccttcagc gccatcatcg
       61 tgttctggtg aaagtggtag acgcggtcgg ccttggtcgg cacgtcgagt gccttcatcc
      121 gctgcgcgtc ctgggtggtc acgccggtcg ggcaggcgcc ggtatggcag gtctgcgcct
      181 ggatgcagcc gagcgcgaac atgaagccgc gggcggcgtt gcaccagtcg gcaccgagcg
      241 ccagcatccg ggcgatgtcg aaggcgctga ccaccttgcc ggcgcagccc agtctgatct
      301 tcttgcgcag gtcgagcccg accagggtgt tgtgcaccag cagcaggcct tcctgcagcg
      361 gcatgccgac gtggtcggtg aactcgagcg gcgccgcgcc ggtgccgcct tcggcgccgt
      421 cgacgacgat gaagtcgggg gtgatgccgg tttccagcat cgccttggcg atgccgaacc
      481 actcccagac atggccgatg cagagcttga agccggtggg cttgccgccg gagagctcgc
      541 gcagccgctc gatgaaatgc atcatctcga tcggcgtcct gaaggcgccg tgcgaggccg
      601 gcgagacgca gtcgacccag ggctggacat ggcgtgcctc ggcgatctcg agcgtcacct
      661 tcggcccggg cagcaccccg ccgtgacccg gcttggcgcc ttgcgagagc ttgagctcga
      721 tcatcttcac ctgcggatcg cgggcgttgg cggtgaaata ctcttcgctg aacgagccgt
      781 cctggttgca gcagccgaag tagccggagc cgatctccca gatcaggtcg ccgccgttca
      841 cccggtggtg ccggctgatc gagccctcgc cggtgtcgtg cgcgaagttg ccgcgcttgg
      901 cgccggcgtt gagggcgagg atggcgttgg ccgagagcgc gccgaagctc atcgccgaga
      961 tgttgaagat gctggcgctg tagggttggg cccggccggc gccgaccagc acccggaagt
     1021 cgtgcgtctt cagcagcgac ggctgcagcg aatggttgat ccactcgtag ccgaccgcgt
     1081 tgacgtcgag ctgggtgccg aacggtcgtt tgtcggagtc gcccttggca cgctggtaga
     1141 cgagcgaacg ctgctggcgg gagaacggcg ccgcgtcgcg gtcgtcctcg atgaagtact
     1201 ggcgcatctc cgggcggatg aactcgagca ggaagcgcag gtggccgatc accgggtagt
     1261 tgcgcaggat cgcgtgcttc gtctggatga cgtcgcgcag gccggtcagg accagcacgc
     1321 cgaagacgat cgccagccag ggctcgaagc ccatccgcat ccagcccgcc atgctggcga
     1381 ggaaggcgac gatgcagagc gcccaggcgc cgtagcggat cgggaagtag tggtcgagcc
     1441 ggttgatcca cgcgaacatg gcgctcctcc tcgggcgtgg cgcgatggta ggcccgcccc
     1501 gctgtgcggg cctcgtggaa agcgtgaagg ccttgacagg cctacgcggc gaggaagaag
     1561 tcgcgcaggc gccgcgcggt gtcgtcgggc agctcctcgg gaatgaagtg accggcctcc
     1621 atcgccgcgc cctcgactcg ccccgcggcg cgcgcgcgcc agagcgcgag cgggtcgaag
     1681 cgcttgccga ccaggccgcg cgaaccccag agcaggtaca ggtcgcaggc gatcttctcg
     1741 tccgcgctgc gcgacgcgcg atcgtgctcg aggtcgatcg acgcgccggc gcggtagtcc
     1801 tcgcaggtgg cgtggatcgt gtcggcgcgg cagaagcagc gctcgtattc ggccagcgcc
     1861 tcgggctcga tgaagtcgat gccgttcgtg ccccagccac cgagcgtcca gcgcaggtag
     1921 aacgcggggt cgccgccgat catccgctcg ggcagcggcg ccggctggat caggaagaac
     1981 cagtggtagt accggctggc aaagcgcatg tcggtcgccg cgtacatgtc gagcgtcggc
     2041 gcgatgtcga tcaccgccag ccgcgcgacg cgctcgggat ggtcgagcgc gagccggtgc
     2101 gcgacccggc cgccgcggtc gtggccggcg acggcgaagc gctcgtggcc gagcgcgctc
     2161 atcagctcgg cggcgtcgag cgcgagcgcg cgcttctcgg caatcgccga ccggaacgcc
     2221 gcggcgccga acatcttgag cgacagccag acgcgcaggc ctgggaatcc gcgcgacagc
     2281 tcgggaccgt gctggctcgg gtcgtagaaa tccgacgggt ggggcatcgc cggcaggtag
     2341 tcggcggtcg cctcgtgcgc cgcccgcagc gccgcgccgt cgcgcaccag cagcgcgccg
     2401 gtgccgtacg gcagaaacat ccccttgtgc ggatcgagcg tcagcgagtc ggcgcgcggc
     2461 aggccgcgca gc
//
LOCUS       DASRDG010000504         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1001625, whole genome shotgun sequence.
ACCESSION   DASRDG010000504 DASRDG010000000
VERSION     DASRDG010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001625"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /gene="serC"
                     /locus_tag="VFA35_08480"
     CDS             <1..925
                     /gene="serC"
                     /locus_tag="VFA35_08480"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZT56246.1"
                     /translation="EALLRELMAIPANYKVLFMQGGAIGENAIVPMNLLRGKDRADYI
                     HTGEWSKKSIAEAKRYCKVNVAASAEASGFTSVPPRESWKLDADAAYVHICANETIGG
                     VEYHFTPDTGAVPLVADMSSNILSRPVDVARYGLIYGGAQKNIGPAGLTLVIVRDDLI
                     GQAHPLTPSAFDYQQQAANDSMLNTPPTYAIYIAGLVFRWIKARGGLAAMAEHNRAKA
                     SVLYDYLDQGALYRNPVARADRSLMNVPFKLKDETLDDAFLKGAQAAGMVQLKGHRSV
                     GGMRASIYNAMPIEGVQALVAYMKEFEARHG"
     gene            948..2129
                     /locus_tag="VFA35_08485"
     CDS             948..2129
                     /locus_tag="VFA35_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZT56247.1"
                     /translation="MAKRLGILVLNHISASGLSRLPAETYSVGKDVADPVAILLRSAD
                     LHTMAIPASVCAIGRAGVGTNNIPVEAMSRRGVPVFNAPGANANAVKELVLAGMLLAA
                     RQIAPAMRFVAALDTAVPDLDQAVEREKKAFAGYELAGQTLGIIGLGKIGCLVADAAI
                     KLGMNVLGFDPDITVDAAWSLPSQVRRATSVNEVLKRAQFVNLHVPLLEATRHLVNDA
                     NIGLMRPGAVLLNFSREGVVSDSAVLDALSANRLGQYVCDFPGASIHGHPQVIALPHL
                     GASTREAEENCAVMVVDQLRDYLEHGNLSNAVNFPQVSMARESAYRVAIANANVPNML
                     GQISTAMAHAGLNIHNMINKSRGDMAYTLVDVDSPVLPEVQAGLVAIDGVLAVRYLPA
                     H"
BASE COUNT          374 a          782 c          731 g          324 t
ORIGIN      
        1 cgaggcgctg ctgcgcgaac tgatggccat cccggcgaac tacaaggtgc tcttcatgca
       61 gggcggagcg atcggcgaga acgcgatcgt gccgatgaac ctgctgcgcg gcaaggacag
      121 ggccgactac atccacaccg gcgagtggtc gaagaagtcg atcgccgagg cgaagcgcta
      181 ctgcaaggtg aacgtcgccg ccagcgccga ggcgagcggc ttcacctcgg tgccgccgcg
      241 cgagagctgg aagctcgacg ccgatgccgc ctacgtgcac atctgcgcca acgagacgat
      301 cggcggcgtc gaataccact tcacgcccga caccggcgcc gtgccgctgg tggccgacat
      361 gtcgagcaac atcctctcgc gtccggtcga cgtcgcccgc tacggcctga tctacggcgg
      421 cgcgcagaag aacatcggcc ccgccgggct caccctcgtg atcgttcgcg acgacctgat
      481 cggccaggcg cacccgctca cgccctcggc cttcgactac cagcagcagg ccgccaacga
      541 ctcgatgctc aacacgccgc ccacctacgc gatctatatc gccgggctcg tgttccgctg
      601 gatcaaggcc cgtggcggcc tggcggcgat ggccgagcac aaccgtgcca aggcgtcggt
      661 cctctacgac tacctcgacc aaggcgccct gtaccgcaat cccgtggcgc gcgccgatcg
      721 ctcgctgatg aacgtgccct tcaagctgaa ggacgagacg ctcgacgacg ccttcctgaa
      781 gggcgcgcag gccgcgggca tggtgcagct gaagggccat cgctcggtcg gtggcatgcg
      841 cgcctcgatc tacaacgcga tgccgatcga aggtgtgcag gcgctggtgg cctacatgaa
      901 agagttcgag gcccgccatg ggtaagcctg ggcatgcaag cccggctgtg gccaagcggc
      961 tcggcattct ggtgctcaac cacatctcgg cctcgggcct ctcgcgcctg ccggccgaga
     1021 cctacagcgt cggcaaggac gtggccgatc cggtggcgat cctgctgcga tcggccgacc
     1081 tgcacacgat ggcgattccc gccagcgtat gcgcgatcgg ccgcgccggc gtcggcacca
     1141 acaacatccc ggtcgaggcg atgagccggc gcggcgtgcc ggtgttcaac gcccccggcg
     1201 ccaacgccaa cgcggtgaag gagctggtgc tcgccggcat gctgctcgcg gcgcggcaga
     1261 tcgcgccggc gatgcgcttc gtcgccgcgc tcgatacggc cgtgcccgac ctcgaccagg
     1321 cggtggagcg cgagaagaaa gccttcgccg gctacgagct ggcggggcag acgctcggca
     1381 tcatcggcct gggcaagatc ggctgcctgg tcgccgacgc cgccatcaag ctgggcatga
     1441 acgtgctcgg cttcgacccc gacatcacgg tcgacgcggc ctggagcctg ccctcgcagg
     1501 tgcgccgcgc gaccagcgtc aacgaggtgc tcaaacgggc ccagttcgtg aacctgcacg
     1561 tgccgctgct cgaggcgacg cgccacctcg tcaacgacgc caacatcggc ctgatgcggc
     1621 ccggcgccgt gcttctcaac ttcagccgcg agggcgtcgt cagcgacagc gcggtgctcg
     1681 acgcgctctc ggccaaccga ctcggccagt acgtctgcga ctttcccggc gcgtcgatcc
     1741 acggccatcc gcaggtgatc gccctgccgc acctcggcgc ctcgacgcgc gaggccgagg
     1801 agaactgcgc ggtgatggtg gtcgaccagc tgcgcgatta cctcgagcac ggcaacctca
     1861 gcaacgcggt caacttcccg caggtgagca tggcgcgcga gtcggcctac cgggtggcga
     1921 tcgccaacgc caacgtgccc aacatgctcg gccagatctc caccgcgatg gcccacgccg
     1981 gcctgaacat ccacaacatg atcaacaagt cgcgcggcga catggcctac acgctggtcg
     2041 acgtcgacag cccggtgctg cccgaagtgc aggccgggct ggtcgcgatc gacggcgtgc
     2101 tggcggtgcg ctacctgccc gcgcactgag ggcggctcgt cagcggaaca ggttctgcgg
     2161 gatgtttgcc gccctgaaga tgtagtctcg ggaatcctgg agctgcgtgc g
//
LOCUS       DASRDG010000505         4463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1459305, whole genome shotgun sequence.
ACCESSION   DASRDG010000505 DASRDG010000000
VERSION     DASRDG010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459305"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFA35_08490"
     CDS             <1..702
                     /locus_tag="VFA35_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HZT56248.1"
                     /translation="AAGRLKLEQGLQKIEFAPRAGRLLRFRVLSVQNPEGDGASDTDP
                     MVSAAQGGTARAFDALRPRDVGPIALSTFHILEHRVDERAQQQRPLSELALPPASAKN
                     VARDRAFGGNAEMRMNGLRFRRGLGVGATSRIDVKLDGNWQLLRADLGIDDSCRDAGG
                     LQFQVWGDERLLYDSGLVKAPGVVKPELDIRGLHRLSLRTLGAQGPRAPEVCGNWANA
                     LLIGQEGDTASIVSP"
     gene            786..3764
                     /locus_tag="VFA35_08495"
     CDS             786..3764
                     /locus_tag="VFA35_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZT56249.1"
                     /translation="MSSSRHYRIALTRLSMALAAAVAAIPLAAQEASPDAGKKDDKTT
                     TQMERIEVTGSNIKRTDVETASPLQIITKAEIEDMGARTLLQVLDNLPAARPAQQDYK
                     SLFTGSDGASQANLRGLGAQGTLVLMNGRRLSYYGAPAGFQTQFVNIDSIPAAAIERV
                     EVLTDGASAVYGTDAVAGVINVITKSSYQGAELSLSSDSSTRIGSYGERQASITGGFG
                     NLAEDRYNVYGSVNLYRRDPIPLSDWYDKKPAQFYVNNPNYITNFRLGVGSAPGVFNP
                     GSYFAFDPKTKARVQEAAPGCNTAIVEATGPRCVWQTWLNNQIDAGAQSDRTTGYLHG
                     TFQVTDSLQAFGEITYTDIDLKANGGTPRAFGTTTGNPTSWFARNTGTSVNQFFYPYL
                     GPNNEYNHASAALKSLMGGVVGLQYLMQDVGNSYYGQRNTDKSYRALGGLRGSFGDWS
                     WETALTTAGSASVTYQTVNVNIDGFKKAFGPFTVDPGTGRIIISDHPAYKFGVISEEN
                     AKLLREAYPTFDIRSWTRLTTWDGKVEGPLATLPAGEMRAAFGFSVAHESFYTPGNTD
                     AANGLITQQGGSWFDGKRTTGAVFGEAVAPITDKIEVDGALRVDKYPNFDANVAPKIG
                     IKFQALPELLLRGTYSEGFRAPSLAEAGTGGVFAQLGGYRDDIRCGETNAIANLLLKS
                     KRPGDVDLGKALLNTDCSRTVARMTQPNPNLQPEKAKISTLGFVYEPIKQLSVSADYW
                     FIYRRNEIAAPDFTREADIVAKTRFPITDTDRANVAALAAMCADPASGVSCPGTLPGY
                     SMGNVSSVIGQYKNRGRTLVDGFDIDARARFPLDRWGKLTLGAAVTIANRNTAYYDDE
                     TRWYYGNFIGYYGYPKVRATLNADWNYGNWTTSLFFNYTGKTKWAWDQTDTDNTPENC
                     TAGGLALPSNLCGGVPAWWTANLGVTWKPVERLQAGLTIKNLFNRLPFYDPNSFLGDA
                     SDYTNNYGRVYSVSVTYKFL"
     gene            3950..>4463
                     /locus_tag="VFA35_08500"
     CDS             3950..>4463
                     /locus_tag="VFA35_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56250.1"
                     /translation="MRARCFCCTPPLSIVRGSAGKSPLQTARDAGKADLRLADFQPRS
                     MLRVRETRIDRPRFPVIDVHTHLSWMSATRSGVSVGEEMTWFASPEELLPLMERKGIR
                     AMVNLTGGIGKGLEATIARFDHAAPGRLLTMTEPSFEYVAEPDYPQRQADALAHAKRA
                     GACGLKLLKTL"
BASE COUNT          828 a         1556 c         1400 g          679 t
ORIGIN      
        1 gccgctggcc ggttgaagct cgagcaaggt ctgcagaaga tcgaattcgc gccgcgcgcc
       61 ggccgcctgc tgcgtttccg cgtgctcagt gtgcagaacc ccgaaggcga cggcgccagc
      121 gacaccgatc cgatggtcag cgcggcacag ggcggcacgg cgcgcgcgtt cgacgcgctg
      181 cggccgcgcg acgtcggccc gatcgcgctg tcgacgttcc atatcctcga gcaccgcgtc
      241 gacgaacgcg cgcaacagca gcgcccgttg tccgagctgg ctctgccgcc ggcatcggcg
      301 aagaatgttg cgcgagatcg tgccttcggc ggcaacgccg agatgcgcat gaacggcctg
      361 cggttccgcc gcggcctcgg cgtcggcgcg acgagccgca tcgacgtgaa gctcgacggc
      421 aactggcaac tgctgcgcgc cgacctcggc atcgacgaca gctgccgcga tgccggcggc
      481 ctgcagttcc aggtctgggg cgacgagcgc ctgctctacg acagcggcct ggtgaaagcc
      541 ccgggcgtgg tcaaacccga actcgacatt cgcggcctgc accggttgag cctgcgcact
      601 ctgggcgcgc agggcccgcg cgcgcccgag gtctgcggca actgggccaa cgccttgttg
      661 atcggccagg aaggcgacac cgccagcatc gtttcaccct gatccatttt tcattcccat
      721 cgcacgacac ccccgtaaac catccaacgc cagagcggct tccgctgccg aacgaggaaa
      781 cgaaaatgtc ttccagccgt cactaccgca tcgccctgac ccgcctgtcc atggcccttg
      841 ccgcggccgt tgccgctatt ccgcttgccg cgcaggaagc gtcgccggat gcaggcaaga
      901 aggatgacaa gacaaccacg caaatggagc gcatcgaggt caccggttcc aacatcaagc
      961 gcaccgatgt cgaaaccgcc tcgccgctgc agatcatcac caaggccgag atcgaggaca
     1021 tgggcgcgcg caccttgctg caggtgctcg acaatctgcc ggccgcgcgc ccggcgcagc
     1081 aggactacaa gtcgctgttc accggctcgg acggcgcttc gcaggccaac ctgcgcggcc
     1141 tcggcgcgca aggcacgctg gtgctgatga acggtcgccg cctgtcgtac tacggtgcgc
     1201 ccgcgggctt ccagacccag ttcgtcaaca tcgactcgat tcccgccgcc gcgatcgaac
     1261 gcgtggaagt gctgaccgac ggcgcctcgg ccgtgtacgg caccgacgcg gtggccggcg
     1321 tgatcaacgt catcaccaag agttcctacc agggcgccga actgagcttg tcttccgaca
     1381 gctccacgcg catcggctcg tacggcgagc gccaggccag cattaccggc ggcttcggca
     1441 atctcgccga agatcgctac aacgtctacg gctcggtcaa cctgtaccgg cgcgatccga
     1501 tcccgctctc cgactggtac gacaagaagc cggcccagtt ctatgtcaac aacccgaact
     1561 acatcacgaa tttccgcctc ggcgtcggca gcgcgccggg cgtgttcaat cccggctcct
     1621 atttcgcctt cgatccgaag accaaggcgc gcgtccagga agcggcgccc ggctgcaaca
     1681 ccgcgatcgt cgaagccacc ggcccgcgct gcgtgtggca gacctggctg aacaaccaga
     1741 tcgatgcggg cgcgcagtcg gatcgcacca ccggctatct ccacggcacg ttccaagtga
     1801 ccgattcgct gcaggcgttc ggcgagatca cctacaccga catcgacctc aaggccaacg
     1861 gcggcacgcc acgcgccttc ggcaccacca cgggcaaccc gaccagctgg ttcgcgcgca
     1921 acaccggcac cagtgtcaac cagttcttct acccgtacct tggcccgaac aacgagtaca
     1981 accacgccag cgccgcgttg aagtcgctga tgggtggggt cgtgggcctg caatacctga
     2041 tgcaggacgt gggcaacagc tactacggtc agcgcaacac cgacaagagc taccgcgcgc
     2101 tcggcggtct gcgcggcagc ttcggcgact ggagctggga aaccgcgctc accaccgcag
     2161 gctccgcgtc ggtcacctac cagaccgtca acgtcaacat cgacggcttc aagaaggcgt
     2221 tcggcccgtt caccgtcgat ccgggcactg gccgcatcat catctccgat catccggcgt
     2281 acaagttcgg cgtgatcagc gaggagaacg ccaagctgct gcgcgaagcc tatccgacct
     2341 tcgatatccg ctcgtggacg cggctgacca cgtgggacgg caaggtcgaa ggcccgctgg
     2401 ccacgctgcc cgccggcgaa atgcgcgcgg cgttcggctt cagcgtggcg cacgagtcgt
     2461 tctacacgcc gggcaacacg gatgcggcga acgggctgat cacgcagcag ggcggttcgt
     2521 ggttcgacgg caagcgcacc acgggcgcgg tgttcggcga agcggtcgcg ccgatcaccg
     2581 acaagatcga agtcgacggc gcgctgcgcg tggacaaata tccgaacttc gacgccaacg
     2641 tcgcgccgaa gatcggcatc aagttccagg cgctgccgga actgctgttg cgcggcacct
     2701 attccgaagg tttccgcgca ccgagcctgg ccgaggccgg caccggcggc gtcttcgccc
     2761 agctcggcgg ctaccgcgac gacatccgct gcggcgagac caacgcgatc gccaacctgc
     2821 tgttgaagtc gaagcggccg ggcgacgtgg atctgggcaa ggcgctgctc aacaccgatt
     2881 gcagccgcac ggtcgcgcgc atgacccagc cgaacccgaa cctgcagccg gaaaaggcca
     2941 agatcagcac gctcggtttc gtgtacgagc cgatcaagca attgtccgtg tcggccgatt
     3001 actggttcat ctaccgccgc aacgagatcg ccgcgccgga tttcacccgg gaagcggaca
     3061 tcgtggcgaa gacgcggttc ccgatcaccg acaccgatcg cgcgaacgtg gcagcgcttg
     3121 ccgccatgtg cgccgatccg gccagcggcg tgagctgccc cggtacgctg ccgggctaca
     3181 gcatgggcaa tgtgtccagc gtcatcggcc agtacaagaa ccgcggccgc accctggtcg
     3241 acggcttcga catcgacgcg cgcgcccgct ttccgctcga tcgctggggc aagctgaccc
     3301 tcggcgcggc cgtgacgatc gctaaccgca acacggccta ttacgacgac gagacccgct
     3361 ggtactacgg caacttcatc ggctactacg gctacccgaa agtgcgcgcc acgctcaacg
     3421 ccgactggaa ctacgggaac tggaccacga gcctgttctt caactacacc ggcaagacca
     3481 agtgggcctg ggaccagacc gacacggaca acacgccgga gaactgcact gccggcggtc
     3541 tggccttgcc ctccaacctg tgcggtggcg tgccggcgtg gtggacggcc aacctcggcg
     3601 tgacctggaa gccggtggag agactgcagg ccggcctcac gatcaagaac ctgttcaatc
     3661 gcctgccgtt ctacgatccg aacagcttcc tcggcgacgc cagcgactac acgaacaact
     3721 acggtcgcgt ctacagcgtc tcggtgacct acaagttcct gtaagcgctg cgtcgccatc
     3781 aaggccgatg aaaacggctt ttgattggca tcgaaacctc ggccgccatt ccttcacgct
     3841 gtcaggcggg aatggtgggc gagggcgttg tcgcactatg cttcgatgcc gaagacgggg
     3901 cagtacgatg aaaaccgcaa gcaaaatcca atccgccgcc gcgccgccga tgcgggcgcg
     3961 atgtttctgc tgcacgccgc cgctgtcgat cgtgcgcggc agtgccggca agagtccact
     4021 gcagaccgcg cgcgatgcgg gcaaggcgga tctgcgcctg gccgacttcc agccgcgcag
     4081 catgctgcgc gtgcgcgaga cgcgcatcga ccgcccgcgc tttccggtga tcgacgtgca
     4141 cacgcacctg tcgtggatga gtgcgacgcg cagcggcgtt tccgtcggcg aggagatgac
     4201 ctggttcgcc tcgcccgagg aattgctgcc gctgatggag cgcaagggca tccgcgcgat
     4261 ggtcaacctg accggcggca tcggcaaggg gctggaggcg acgatcgcgc gcttcgacca
     4321 cgccgcgccc gggcgtctgc tgacgatgac cgagccgtcg ttcgaatacg tcgccgagcc
     4381 cgactacccg cagcgccagg ccgatgcgtt ggcgcacgcc aagcgcgccg gcgcctgcgg
     4441 gctcaagctg ctgaagacgc tcg
//
LOCUS       DASRDG010000506         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_648588, whole genome shotgun sequence.
ACCESSION   DASRDG010000506 DASRDG010000000
VERSION     DASRDG010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648588"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..2002
                     /locus_tag="VFA35_08505"
     CDS             68..2002
                     /locus_tag="VFA35_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT56251.1"
                     /translation="MAILSLSNAHLAFGHVALLDNAAFSLETGERLGLIGRNGAGKSS
                     LLKIVAGLEKLDDGLLQMTQGVRICYVPQEPVFEPGHSVFEAVSEGVAEARAVRQAYE
                     EHADGVDLDALQTRIEALDAWNWEQRVDTTLAQLHLDGTREIGQLSGGMKKRVALAQA
                     LVAVPDVLLLDEPTNHLDLDSIAWLEELLRGFKGAVMLITHDRAFLDGMATRIIELDR
                     GIIRSYPGNFSAYERMKEEQLASDALANARADKLLAQEEVWVRKGVEARRTRSVARIQ
                     RLEVLRDQRRKRRDSLGQVRLEVDSGAPSGKIVAELKEVSLSFRDHAAPKVIVKDFSA
                     TILRGDKVGLIGPNGAGKTTLLKLILGELQPDSGTVRQGSKLEVAYFDQMRSTLNLDA
                     TLADTISPGSEWVEFAGQRKHVMSYLNDFLFSPERANSPVRTLSGGERNRVLLARLFA
                     LPANVLVLDEPTNDLDIDTLELLEELLQAYKGTVFLVSHDRRFLDNVVTSTIVWEGDE
                     SPGRWREYEGGYEDWKLQRDRAQAIRDQAARAAKSKDARANYALPAAKAAAVSAAAPA
                     PVAAKPRKLSYKEQRELDELPKRIEALEAEQNELASFLARPESYAKEAERALQAQTRV
                     AAIDDELLAALERWEILGAR"
     gene            complement(2009..>2529)
                     /locus_tag="VFA35_08510"
     CDS             complement(2009..>2529)
                     /locus_tag="VFA35_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56252.1"
                     /translation="TIHLQCGERVLPLTVFPHERLVHCPMEIGELVERHLAELRVMHV
                     EPATLRPPGDPQHELVGEDRLHHPLTPLLWALALRGPRRDLLPEIAGPAVYRVSPALD
                     IDALPVSGAIRAAVNRLRRQATALGGIAAWPAFDRERAARLLNALYLQAGLIVSRSHP
                     DAVRDGWFGTKS"
BASE COUNT          421 a          883 c          884 g          341 t
ORIGIN      
        1 agtcactggg ataatcctcg tttccccgag agcctcgcgc gtgatgcgcg ctggccgcgc
       61 gcgaccgatg gccattcttt ccctctccaa cgcccacctc gccttcggcc atgtggccct
      121 gctcgacaac gccgcgttct cgctcgagac cggcgagcgc ctgggcctga tcggccgcaa
      181 cggggccggc aagtcgtcgc tgctgaagat cgtcgccggc ctcgagaagc tcgacgacgg
      241 gctgctgcag atgacgcagg gcgtgcgcat ctgctacgtg ccgcaggagc cggtgttcga
      301 gcccggccac agcgtgttcg aggccgtgag cgagggcgtg gccgaggcgc gcgcggtgcg
      361 ccaggcttac gaggaacacg ccgatggcgt cgacctcgac gcgctgcaga cccgcatcga
      421 agcgctcgac gcgtggaact gggagcagcg cgtcgacacc acgctcgcgc agctgcacct
      481 cgacggcacg cgcgagatcg gccagctctc gggcgggatg aagaagcgcg tcgcgctcgc
      541 gcaggcgctg gtcgcggtgc ccgacgtgct gctgctcgac gagccgacca accacctcga
      601 cctcgattcc atcgcgtggt tggaggagct gctgcgtggg ttcaagggcg ccgtgatgct
      661 gatcacccac gaccgcgcgt tcctcgatgg tatggccacc cggatcatcg agctcgaccg
      721 cggcatcatc cgcagctacc cgggcaactt cagcgcctac gagcgcatga aggaagagca
      781 gctcgcctcg gatgcgctcg cgaacgcgcg cgccgacaag ctgctcgcgc aggaggaggt
      841 gtgggtccgc aagggcgtcg aggcgcgccg cacgcgcagc gtcgcgcgca tccagcgtct
      901 cgaagtgctg cgcgaccagc gccgcaagcg ccgcgattcg ctcggccagg tgcggctcga
      961 ggtcgattcg ggcgcgccca gcggcaagat cgtcgccgag ctgaaggagg tctcgctgag
     1021 cttccgcgac cacgccgcgc cgaaggtcat cgtcaaggat ttcagcgcga cgatcctgcg
     1081 cggcgacaag gtcggcctga tcggtccgaa cggcgccggc aagacgacgc tgctcaagct
     1141 catcctcggt gaactgcagc ccgacagcgg cacggtgcgc cagggcagca agctcgaagt
     1201 cgcgtacttc gaccagatgc gcagcacgct gaacctcgac gccacgctcg ccgacacgat
     1261 cagcccgggc agcgagtggg tcgagttcgc gggccagcgc aagcatgtga tgagctacct
     1321 gaacgacttt ctgttctcgc ccgagcgcgc gaactcgccg gtgcgcacgc tcagcggcgg
     1381 cgagcgcaac cgcgtgctgc tcgcgcgcct gttcgcgctg cccgcgaacg tgctggtgct
     1441 cgacgagccg accaacgacc tcgacatcga cacgctcgag ctgctcgaag agttgctgca
     1501 ggcctacaag gggaccgtct tcctcgtcag ccacgaccgg cgctttctcg acaacgtcgt
     1561 gaccagcacg atcgtctggg aaggagacga gtcacccggc cgctggcgcg agtacgaagg
     1621 cggctatgaa gactggaagc tccagcgcga ccgcgcgcag gcgatccgcg accaggcggc
     1681 gcgcgcggcg aagtcgaagg acgcgcgcgc aaactacgcg cttccggctg caaaggccgc
     1741 tgcggtatct gcggcagcgc ctgcgccggt cgccgccaag ccgcgcaagc tcagctacaa
     1801 ggagcagcgc gagctcgacg agctgccgaa gaggatcgag gcgctcgaag ccgagcagaa
     1861 cgaactcgcg agctttctgg cgcggcccga gtcgtacgcg aaagaggccg agcgcgcgct
     1921 gcaggcgcaa acccgcgtgg cggcgatcga cgacgagttg ctcgccgcgc tggagcgctg
     1981 ggaaattctc ggggcgcgtt gagccgcgtc aggacttggt cccgaaccag ccgtcgcgca
     2041 ccgcatccgg gtgcgagcgg ctgacgatca gcccggcctg caggtacagc gcgttcagca
     2101 gccgcgccgc gcgctcgcgg tcgaacgccg gccaggcggc gatgccgccg agcgcggtcg
     2161 cctgccggcg cagccgattg accgctgcgc ggatcgcgcc gctgaccggc agcgcgtcga
     2221 tgtcgagcgc tggcgacacc cggtagaccg ccggcccggc aatctcgggc agcaggtcgc
     2281 ggcgcggccc gcgcagcgcc agcgcccaca gcagcggcgt cagcggatgg tgcagccggt
     2341 cttcgccgac cagctcgtgc tgcgggtcgc cgggcgggcg cagggtcgcc ggctcgacgt
     2401 gcatcacgcg cagttcggcc aggtggcgct cgaccagctc gccgatctcc atcgggcaat
     2461 gcaccagccg ctcgtgcggg aagacggtca gcggcaagac ccgctcgcca cactgcaagt
     2521 gaatggtca
//
LOCUS       DASRDG010000507         4689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1229090, whole genome shotgun sequence.
ACCESSION   DASRDG010000507 DASRDG010000000
VERSION     DASRDG010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229090"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1041)
                     /locus_tag="VFA35_08515"
     CDS             complement(133..1041)
                     /locus_tag="VFA35_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HZT56253.1"
                     /translation="MKSLILALSFAVASAGLVAPFEAEAKRLGSGTSSGMQRSMPARA
                     PAPTTPAQPAQAAPTAPATAGAAPAAAAPKRSWMGPIAGLAAGLGLAALMSHFGMGEA
                     FGNILTMVLLALVAFVAIRFLMNRFARNSAPALATPNGMPFNGAPPAAANNTSAWTQP
                     APGAAVLPATAAATLPAGFDTAGFERIAKTIFIRMQAANDNADLNDLRAFTTPEMFAA
                     VKLDLQERGGAAQTTDVVRIDAEVLDVVSEPERQIVSVRFHGLIREEKDGVAAPFDEV
                     WHLVKPADGSREWAIAGIQQTTPALA"
     gene            1480..2580
                     /gene="rsgA"
                     /locus_tag="VFA35_08520"
     CDS             1480..2580
                     /gene="rsgA"
                     /locus_tag="VFA35_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZT56254.1"
                     /translation="MIDIDFERLRTLGLTSALAHAAAQAAADADASPPLELLRLTEVH
                     RDAVRLHDGADELMARVPPRLTRTLADDGAMLAVGDWVLAATDAHGQIHVHARVPPLT
                     RLNRRDADGRRHTVVANVDTALLVMGLDDDFNPRRLERYLALVRAGGAGITPAVVLTK
                     ADVIASVPGRLAERLEALRGRVPARIDLFAIDGTDPYTVQQLQAHLGAGQTLVVLGSS
                     GAGKSTLTNTLLGRAVQDTGAVRVHDGRGKHTTTARSLHRLPGGACVIDTPGLRALRP
                     DVDAATLAAAFDDIESLALRCRFRDCRHVDEPGCAVRAGVPADRVTNYHKLLREARRD
                     TLSVLERRQQLALWKARGRAGRERLKHKRDGV"
     gene            complement(2588..3223)
                     /locus_tag="VFA35_08525"
     CDS             complement(2588..3223)
                     /locus_tag="VFA35_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZT56255.1"
                     /translation="MSAAVEPVHVDAHCIVAVKPSGLLAVPGRGAHGQDCLAARVQAG
                     FADALVVHRLDMSTSGLMLFARGAPSQRALSSAFAARAVHKRYVAVVHGRVDAAQGEI
                     DLPLGADWPNRPKQKVDRAAGKPSLTRWRVLVFDAARGTTRLELEPVTGRAHQLRVHL
                     LAIGHPIVGDALYAPPEVIAQADRLLLHASSLRFTHPHTGEAIAFESAAPF"
     gene            complement(3233..3934)
                     /locus_tag="VFA35_08530"
     CDS             complement(3233..3934)
                     /locus_tag="VFA35_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZT56256.1"
                     /translation="MGTTSSAPRRAAKRGRSVARTALLAGATGLIGRALLPLLQASPA
                     YAHTHVLVRRAPAAAAADARTSFHVVDFAQLPALPAVDDCYIALGTTIKVAGSEAAFR
                     AVDFDYVVNTARAARAAGATRLAVVSALGADAHSAVFYNRVKGEMQAAVGALGFASVV
                     IAQPSLLLGDRAAIGQPPRPAEVVAARLLAPAMWLVPRAVRPIAATQVAAALLGATLA
                     AAPGVHVLSSSQMQG"
     gene            complement(3991..>4689)
                     /locus_tag="VFA35_08535"
     CDS             complement(3991..>4689)
                     /locus_tag="VFA35_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZT56257.1"
                     /translation="PVGRELKKDLLVHLIKSNDWDQVLVFTRMKHGANRLVEHLLKEN
                     ISAMAIHGNKSQTARTKALAEFKTGDLTVLVATDIAARGIDIDMLPHVVNYEMPNVPE
                     DYVHRIGRTGRAGAKGEAVSLVCVDENGFLRDIERLIKREIPKEIVPGFEPHPSEKAE
                     PIVLGRMVIGEGAGRGGQRHHAGGGGAGRGGHGGGGGRGGSGGGQRSGGGGGASRPAG
                     GGGRPPARSGPPKR"
BASE COUNT          638 a         1732 c         1697 g          622 t
ORIGIN      
        1 aaacaggccg cggcgagtcg cgcagcgcgg atcgggatga aaggcatcgg catgaaaaag
       61 ggcgccgcct cgacggcggc gcccatattg tcgtgcgtcg cgaccgcgcc ggcgcggcgc
      121 gaagcgggcc cgtcaggcga gggccggcgt cgtctgctgg atgcccgcga tcgcccactc
      181 gcggctgccg tcggccggct tcaccaggtg ccagacctcg tcgaagggcg cggccacacc
      241 gtccttctct tcgcggatca ggccgtggaa gcgcacgctg acgatctggc gctcgggctc
      301 gctgacgacg tcgagcacct cggcgtcgat gcgaaccacg tcggtcgtct gcgccgcgcc
      361 gccgcgctcc tgcagatcga gcttgaccgc ggcgaacatc tcgggcgtgg tgaaggcgcg
      421 caggtcgttc aggtcggcgt tgtcgttcgc ggcctgcatg cggatgaaga tcgtcttcgc
      481 gatgcgctca aagccggcgg tgtcgaagcc cgcgggcagc gtggcggcgg ccgtggccgg
      541 cagtaccgca gcgcccggtg cgggttgcgt ccacgccgac gtgttgttcg cggcggccgg
      601 cggcgcgccg ttgaacggca tgccgttcgg cgtcgccagc gccggcgcgc tgttgcgcgc
      661 gaagcggttc atcaggaagc ggatcgcgac gaacgcgacc agcgcgagca gcaccatcgt
      721 caggatgttg ccgaacgctt cgcccatgcc gaagtggctc atcagtgcgg ccagccccag
      781 gcccgcggcc aggccggcaa tcgggcccat ccacgagcgc ttcggcgcgg cggcggccgg
      841 tgcggcgccg gccgtcgcgg gcgcggtcgg cgcggcttgt gcgggttgcg ccggcgtggt
      901 gggcgcgggt gcgcgcgccg gcatgctgcg ctgcatgccc gatgacgtgc cactgcccag
      961 gcgcttggct tcggcctcga acggcgcgac caggccggca gacgccacgg cgaacgacag
     1021 ggcgaggatc agggatttca tcagagcatt ccttcggttg gcgggccatc gggcggcacc
     1081 ggcggcgcga tcatacgggg cttgaatgac cccactgagc ccactgccgg cgggtgacat
     1141 ttgcggctgc gttgcgaaaa cacaaggcgg cgattgacgc gggtcgcgcc ggcacgcaca
     1201 ctgcgcgcct tgcgacgccg cccggcctcg cacccgattc gcaggaggtg ccgccatgaa
     1261 cccatcatcg acccttgccc tctcgtcggc cgcggcctga cctgcgcttc gcacgcctcg
     1321 agttccttcc tcacgcccac tcgggtctga ttcccgcgca catcgcgcga ccgacgtttc
     1381 caccccccgc tgacctgcgc gtgcgcttgc acacgccggg tcgaaccgcg cgcggctttc
     1441 gcgccgcgcg ctgcgagaaa cgaaagttca gaccccacca tgatcgacat cgacttcgag
     1501 cgcctgcgca cgctgggcct cacgtccgcg ctggcccatg ccgcggcgca ggctgcggct
     1561 gacgccgacg cttcacctcc cctcgaactg ctgcgcctga ccgaggtgca ccgcgacgcc
     1621 gtgcgcctgc acgacggcgc cgacgaactg atggcgcgcg tgccgccgcg cctgacccgc
     1681 acgctcgccg acgacggcgc gatgctcgcg gtcggcgact gggtgctggc cgcgaccgac
     1741 gcgcacggcc agatccacgt gcatgcgcgc gtgccgccgc tgacccgcct caaccggcgc
     1801 gacgccgacg gccggcgcca cacggtcgtc gccaacgtcg acaccgcgct gctggtgatg
     1861 ggcctcgacg acgacttcaa cccgcgccgg ctcgaacgct acctcgcgct cgtgcgggcg
     1921 ggcggcgccg gcatcacgcc ggccgtcgtt ctcaccaagg ccgacgtgat cgcgagcgtg
     1981 ccaggtcggc tcgccgagcg cctcgaagcg ctgcgcgggc gcgtgccggc gcgcatcgac
     2041 ctgttcgcga tcgacggcac cgacccgtac acggtgcagc agctgcaggc ccacctcggc
     2101 gccgggcaga cgctggtcgt gctcggctcg tccggcgcgg gcaagtcgac cctgacgaac
     2161 acgctgctcg gccgcgcggt gcaagacacc ggcgcggtgc gcgtgcacga cggccgcggc
     2221 aagcacacga ccaccgcgcg ctcgctgcac cgcctgcccg gcggcgcctg cgtgatcgac
     2281 acgccggggc tgcgcgcgct gcggccggat gtcgacgcgg ccacactcgc ggcggcgttc
     2341 gacgacatcg agtcgctcgc gctgcgctgc cgcttccgcg actgccggca cgtcgacgag
     2401 cccggttgcg cggtgcgcgc cggcgtgccg gccgatcgcg tgaccaacta ccacaagctg
     2461 ctgcgcgagg cgcgccgcga cacgctgagc gtgttggaga ggcggcagca actcgcgctg
     2521 tggaaggcgc gcgggcgggc cgggcgcgag cggctgaaac acaagcgcga tggtgtctga
     2581 ccggcgctca gaagggcgcc gcggactcga acgcgatcgc ctcgccggtg tggggatgcg
     2641 tgaagcgcag cgaactcgca tgcagcaaca agcggtcggc ctgcgcgatc acctcaggcg
     2701 gcgcgtacag cgcgtcgccg acgatcgggt gcccgatcgc gagcaggtgc acgcgcagct
     2761 ggtgggcgcg gccggtgacc ggctcgagct cgagccgcgt cgtgccgcgc gccgcgtcga
     2821 agacgagcac gcgccagcgc gtcagcgaag gcttgccggc cgcgcgatcg accttctgct
     2881 tcggccggtt cggccagtcg gcgcccagcg gcaggtcgat ctcgccctgc gcggcgtcga
     2941 cgcggccgtg gacgacggcg acgtaacgct tgtgcactgc gcgcgccgcg aacgcgctgc
     3001 tcagcgcgcg ctgcgatggc gcgccgcgcg cgaacagcat cagccccgag gtcgacatgt
     3061 ccaggcgatg cacgaccagc gcatcggcga acccggcctg cacccgcgct gcgaggcaat
     3121 cctgcccgtg cgcgccgcgc cccggcaccg cgagcaggcc gctgggcttg acggcgacga
     3181 tgcagtgcgc atcgacatgc acgggctcga cggccgcgct catccctgcc gcttatccct
     3241 gcatctgcga cgagctcagc acatgcacgc cgggcgcggc ggccagcgtg gcgccgagca
     3301 gtgccgccgc gacctgggtg gccgcgatcg gccgcaccgc gcgcggcacc agccacatcg
     3361 ccggcgcgag caggcgcgcg gcgacgacct cggccgggcg cggcggctgg ccgatcgcgg
     3421 cgcggtcgcc gagcagcagc gagggctgcg cgatcacgac cgacgcgaag ccgagcgcgc
     3481 cgaccgccgc ctgcatctcg cccttgacgc ggttgtagaa gacggccgag tgcgcgtcgg
     3541 cgcccagcgc cgagacgacg gccaggcgcg tcgcgccggc ggcacgcgcg gcgcgcgcgg
     3601 tgttcacgac gtagtcgaaa tcgaccgcgc gaaatgccgc ctccgagccc gcgaccttga
     3661 tcgtcgtgcc cagggcgata tagcagtcgt cgaccgccgg cagcgcgggg agctgcgcga
     3721 agtcgacgac gtgaaaactc gtgcgcgcat cggccgcagc cgccgccggc gcgcggcgca
     3781 cgagcacatg cgtgtgtgcg taggccgggc tggcttgcag cagcggcagc agcgcgcggc
     3841 cgatcaggcc ggtcgcgccc gcgagcagcg cggtgcgcgc aacagaacgg ccgcgtttcg
     3901 cggcccttcg gggagcagag gaggtggtgc ccatccgggc actgtacccg cgccgccgcg
     3961 acggcgcagg caagagaggt caggagccga tcagcgcttc ggcgggcccg agcgcgcggg
     4021 tgggcgaccg ccgccaccgg ccgggcgcga tgcgccgccg ccaccgcccg aacgctggcc
     4081 gccgccgctc ccgccgcgtc cgccaccacc gccatggccg ccgcggccgg cgccgccgcc
     4141 accggcgtga tgccgttggc caccgcgccc ggcgccttcg ccgatcacca tgcggccgag
     4201 cacgatcggc tcggccttct cgctcgggtg cggctcgaag ccggggacga tctccttcgg
     4261 gatctcgcgc ttgatcagcc gctcgatgtc gcgcagaaag ccgttctcgt cgacgcagac
     4321 cagcgagacc gcctcgccct tcgcgccggc gcggccggtg cgaccgatgc ggtgcacgta
     4381 gtcttcgggc acgttcggca tctcgtagtt cacgacgtgc ggcagcatgt cgatgtcgat
     4441 gccgcgcgcg gcgatgtcgg tcgccaccag cacggtcagg tcgccggtct tgaactcggc
     4501 cagcgccttg gtgcgcgcgg tctggctctt gttgccgtgg atcgccatcg cgctgatgtt
     4561 ttccttcagc aggtgctcga ccagccggtt cgcgccgtgc ttcatgcgcg tgaagaccag
     4621 cacctggtcc cagtcgttcg acttgatgag gtgcacgagc aggtctttct tcagctcgcg
     4681 gccgaccgg
//
LOCUS       DASRDG010000508         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_78383, whole genome shotgun sequence.
ACCESSION   DASRDG010000508 DASRDG010000000
VERSION     DASRDG010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78383"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFA35_08540"
     CDS             <1..967
                     /locus_tag="VFA35_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZT56258.1"
                     /translation="FSHADQAPQWQTARPDDPTAYGWGGRVADLLYSANSGQLPMNIS
                     LSNNNPFLRGASVTAYSMDPGGVDMLNYLGSGSESWIIPDDAQNAAVYNTLIAQGTQA
                     HVLERAFADGARRTIANYSLVNSALNPTPALPALATKFADTPLGNQLKMVAQLIRVRS
                     ALGMSRQVFFVSTGNYDTHANQIADQQGNLDDLSQALNAFYNATVELGVANSVTAFTA
                     SDFGRSLPVNSTGTDHGWGGHHFVIGGAVQGGRFYGTLPSLALNTDVNPNANPDDTGY
                     GQIVPTTSVDQYAATLASWFGVGAADLATIFPNLGRFATANLGFV"
     gene            complement(996..1775)
                     /locus_tag="VFA35_08545"
     CDS             complement(996..1775)
                     /locus_tag="VFA35_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZT56259.1"
                     /translation="MTNHLCRPLAALIFLLAGVFSACATASDHQVTVGGSNDMFSPST
                     LTINAGDTVTFTEVSGSHNVVSDAAGLFRCANGCDGDGKGGNGNVAGNNWSVTINFPT
                     AGTFGYYCEAHGAPGVGMHGTIRVNAVAFGITPGITGLWYNKSQSGHGFDVQVPGNGT
                     IVAIWYVFDNAGNTLWLFGQGTYSGGTATLDVYTSTGGLFPPAFDAATAARNKYKWGT
                     FTLSFSDCNTGTASWVPLVAGYTSGSLPITRLTGVGGLACP"
     gene            complement(1772..>2809)
                     /locus_tag="VFA35_08550"
     CDS             complement(1772..>2809)
                     /locus_tag="VFA35_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZT56260.1"
                     /translation="NGAFVVNVGTALQPTVKTQYLKPGYPVPPQLFSHADQQAQWQYG
                     QPKQNGTTGWGGLVADRLHVLNAGATIPMSISLSGQNRFQAGASVQPYSISASGPASL
                     SGYTGSTGPARITALAEMLAETYADPLSRAYTNKVGNALDYYNTMTAALAQAPALTTV
                     FPANNPVAAALAEVAKVISMRGMLNAKRQIFFIGYGSFDTHANQLNDQPKLLTTVSQA
                     LGAFYDCTVNDLKIPQSITTFTLSEFARTLNSNGDGTDHAWAGNQFVMGGAVQGQKLY
                     GANAASGRLFPDQTLNGPDCLSRGQMIPATSCDQYSATLASWLGVQDCDIATIFPYVG
                     NFAMKNLGFLS"
BASE COUNT          478 a          910 c          936 g          485 t
ORIGIN      
        1 gttttcgcat gcggaccagg cgccgcagtg gcagaccgcg cggccggacg atccgaccgc
       61 gtacggctgg ggcgggcgcg tcgccgatct gctctacagc gcgaattccg gccagttgcc
      121 gatgaatatc tcgttgtcga acaacaaccc gtttctgcgc ggtgccagcg tcaccgccta
      181 ttcgatggat ccgggcggcg tcgacatgct gaactatctc ggcagcggct ccgaatcgtg
      241 gatcatcccg gacgatgcgc agaacgcggc ggtgtacaac accctgatcg cgcagggcac
      301 gcaggcgcat gtactcgagc gcgcgttcgc cgacggcgcg cgacgcacaa tcgcgaatta
      361 ctcgctggtc aattcggcgc tgaatccgac cccggcgctg ccggcgctgg caacgaaatt
      421 cgccgacacg ccgctcggca atcagctcaa gatggtcgcg caattaatcc gcgtgcgcag
      481 cgcgctcggc atgagccggc aggtgttctt cgtctcgacc ggcaactacg acacccacgc
      541 caaccaaatc gccgaccagc aaggcaacct ggacgacctt tcgcaggcgc tgaatgcgtt
      601 ctacaacgcc acggtcgaac tcggcgtggc caacagcgtc accgcgttca ccgcttccga
      661 cttcggccgc tcgctgccgg tgaacagcac cggcaccgac cacggctggg gcggccatca
      721 tttcgtcatc ggcggtgcag tgcaaggcgg ccggttctac ggcacgctgc cttcgcttgc
      781 gctgaacacc gatgtgaacc cgaacgcgaa tccggacgac accggctacg gccagatcgt
      841 gccgacgacg tcagtcgacc agtatgcggc cacgctggca agctggttcg gcgtcggcgc
      901 tgccgatctc gccacgatct tcccgaacct cggccgtttc gcgacagcga atctaggctt
      961 cgtctagcac tggaagccgc gcgaggaaat ctcgcctagg gacaggccag cccaccgacg
     1021 ccggtcaatc gcgtgatcgg cagcgagccg cttgtatagc ccgcgacgag cggcacccac
     1081 gacgcagtgc ccgtgttgca atcgctgaag ctcagcgtga acgtacccca tttatatttg
     1141 ttgcgcgcgg ccgtcgccgc atcgaaggcc ggtgggaaga ggccgccggt cgaggtgtag
     1201 acatccagcg tcgccgtgcc gccgctgtag gtgccctggc cgaacagcca gagggtgttg
     1261 ccggcgttgt cgaacacgta ccagatcgca acgatggtgc cgttgcccgg cacctgcacg
     1321 tcgaagccat ggccggactg gctcttgttg taccagaggc cggtgatgcc cggcgtgatg
     1381 ccgaatgcga ccgcgttgac ccggatcgtg ccgtgcatgc ccacgccggg cgcgccgtgc
     1441 gcctcgcagt agtagccgaa cgtgcccgcg gtcgggaagt tgatcgtcac gctccagttg
     1501 ttgccggcga cattgccgtt gccgcccttg ccgtcgccat cgcagccgtt cgcgcagcgg
     1561 aacaggcccg ccgcgtcgga gacgacattg tgcgatcccg acacctcggt gaacgtgacc
     1621 gtgtcacccg cgttgatcgt cagtgtcgac ggcgagaaca tgtcgttgct gccgccgacc
     1681 gtgacctggt gatcgctggc ggtggcgcac gcgctgaaca cgccagcgag caggaagatg
     1741 agggcggcga gcgggcggca aagatgattg gtcacgacag gaatccgagg ttcttcatcg
     1801 cgaaattgcc gacgtagggg aagatcgtcg cgatgtcgca gtcctgcacg ccgagccagc
     1861 tggcaagcgt cgccgaatac tggtcgcagg aggtcgcagg gatcatttgg ccgcggctca
     1921 ggcagtcggg cccgttgagg gtctggtcgg gaaacaggcg cccgctcgcc gcgttcgcgc
     1981 cgtagagttt ctgcccctgc accgcgccgc ccatcacgaa ctggttgccg gcccaggcgt
     2041 gatcggtgcc gtcgccgttc gaattgagcg tgcgcgcgaa ttcggacagc gtgaaggtcg
     2101 tgatcgactg cggaatcttg aggtcgttga cagtgcagtc gtagaacgcg ccgagcgcct
     2161 gcgacacggt ggtcagcagc ttgggctggt cgttgagctg gttcgcatgc gtgtcgaagc
     2221 tgccgtagcc gatgaagaag atctgccgct tcgcattgag catgccgcgc atcgagatca
     2281 ccttggcgac ttcggcgagc gccgcggcga ccggattgtt cgccggaaat accgtcgtca
     2341 gcgcgggcgc ctgcgcgagc gcggcggtca tcgtgttgta gtaatcgagc gcattgccga
     2401 ccttgttcgt gtacgcgcgc gacagcggat cggcataggt ttcggcgagc atctcggcca
     2461 gcgcggtgat ccgcgccggg cccgtgctgc cggtgtagcc ggagaggctg gccggcccgc
     2521 tagccgagat cgaatacggc tgcaccgacg cgccggcctg gaaccggttc tgaccgctca
     2581 gcgagatcga catcggaatc gtcgccccgg cgttgagcac gtgcagacgg tcggcgacga
     2641 ggccgcccca gccggtcgtg ccgttctgct tcggctggcc gtactgccac tgtgcctgct
     2701 ggtcggcgtg cgagaacaac tgcggcggca ccgggtagcc cggtttgagg tactgcgtct
     2761 tgacggtcgg ctgcagcgca gtgccgacgt tgacgacgaa cgcgccatt
//
LOCUS       DASRDG010000509         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_649218, whole genome shotgun sequence.
ACCESSION   DASRDG010000509 DASRDG010000000
VERSION     DASRDG010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649218"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..466
                     /locus_tag="VFA35_08555"
     CDS             77..466
                     /locus_tag="VFA35_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56261.1"
                     /translation="MRALMLCLALLAGNAAAIDKPAFTEADAVRAVGAGMCGSLPVGT
                     GLVNGRGWIHQPIPGPVQLRLSGAGMVSTIGQPPQRSITLDMFAGRMNYIQTHGTAPG
                     SRLEWKVPGYVWGPVPVGFQLPPVGGK"
     gene            477..842
                     /locus_tag="VFA35_08560"
     CDS             477..842
                     /locus_tag="VFA35_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HZT56262.1"
                     /translation="MTRASATPVRLYGIPNCDTVKKARAWLQAQGREAYFHDFKKAGV
                     PTDRLDAWVDALGWERLVNRQGTTWRRLDAVLQAAVVDAASAKRLMRAEPSVIKRPVV
                     EWADGAVTVGFDAGDWSRR"
     gene            complement(856..1335)
                     /locus_tag="VFA35_08565"
     CDS             complement(856..1335)
                     /locus_tag="VFA35_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZT56263.1"
                     /translation="MLHHSFVRATLAAAALLATLPAWALHPGADGLQWGDAPPVFNKG
                     AKIAVLQGDPGKPGPFVARLSAPSGYKVMPHWHSQVENVTVLSGTLMVGMGDKFDTKV
                     MKTFKAGAFGSIPAQTHHYALFKGATVIQIHGEGPFDLTYVDPADDPQKKMADAKKP"
     gene            complement(1411..>2726)
                     /locus_tag="VFA35_08570"
     CDS             complement(1411..>2726)
                     /locus_tag="VFA35_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZT56264.1"
                     /translation="HIKTSLAPGSRIVTEYLEKAKLLPYLEKLGFNVAAYGCTTCIGN
                     AGDLRPEFNEAIQQNDLICAAVLSGNRNFEARIHPNIKANFLASPPLVVAYAIAGTVL
                     KDPMTEPLGKGKGGRDIYLGDIWPSSDEVHALMKYAMNGKAFKANYDKVATEPGKLWE
                     KIKGVTGQVYNWPRSTYIARPPFFDGFTMEPPELVAGVQGARAMALFGDSITTDHISP
                     AGSIKQSSPAGIWLEANGVGKADFNSYGSRRGNHEVMMRGTFANVRIKNLMIPPSADG
                     SREEGGVSLFQPSGEKMFIYDAAMRYIAEGTPTVIFAGEEYGTGSSRDWAAKGSQLLG
                     VKAVVARSFERIHRSNLVGMGVLPLQFKGADSWESLGIRGDERFDIELSPEIRPQQDA
                     TLVITDTNGRSRTVPLVLRIDTPIEVDYYKHGGILPFVLRQLLAA"
BASE COUNT          386 a          897 c          960 g          483 t
ORIGIN      
        1 gcgtttgcta aagttcggcg cggacgttct tcccgaccgc gcccgacccg atcacccacc
       61 tcacgctcca ccacccatgc gcgcgctgat gctctgcctt gcgctgctcg ccggcaacgc
      121 cgcggcgatc gacaaacccg cgttcaccga ggcggatgcg gtgcgcgcgg tcggtgccgg
      181 catgtgcggt tcgttgcctg tcggcaccgg tctcgtcaat gggcggggct ggatccatca
      241 gccgattccc ggcccggtcc agctgcgcct gtcgggtgcc ggcatggtct cgacgatcgg
      301 ccagccgccg cagcgttcga tcacgctcga catgttcgcc gggcgcatga actacatcca
      361 gacgcacggc accgcgcccg gctcgcggct cgagtggaag gtgccgggct atgtgtgggg
      421 gccggtgccg gtggggttcc agttgccgcc ggtgggcggg aaatagattg ccccggatga
      481 cgcgcgcgtc cgcgacaccc gtgcgccttt acggcatccc gaactgcgac accgtgaaga
      541 aggcgcgcgc ctggctgcag gcgcagggcc gcgaggcgta ctttcacgac ttcaagaagg
      601 ccggcgtgcc gaccgaccgg ctcgacgcct gggtcgacgc gctcggttgg gaacggctcg
      661 tgaaccgcca gggcacgacc tggcgccggc tcgatgccgt gctgcaggcc gcagtcgtcg
      721 atgcggcgag cgcgaagcgc ctgatgcgcg ccgagccgag cgtgatcaag cggccggtcg
      781 tcgagtgggc cgacggcgcc gtcacggtcg gcttcgatgc cggcgactgg tcacgccgct
      841 gagctgcggg cgcgactacg gcttcttggc gtcggccatc ttcttctgcg ggtcgtcggc
      901 ggggtcgacg taggtcagat cgaacggccc ttcgccgtgg atctggatca cggtggcccc
      961 cttgaacagc gcgtagtgat gggtctgggc cgggatcgac ccgaaggcgc cggccttgaa
     1021 ggtcttcatg accttggtgt cgaacttgtc gcccattccg accatcagcg tgcccgagag
     1081 cacggtgacg ttctcgactt ggctgtgcca gtgcggcatc accttgtagc ccgacggcgc
     1141 cgacaggcgg gcgacgaacg ggcccggctt gccggggtcg ccctgcagca cggcgatctt
     1201 ggcgcccttg ttgaacaccg gcggcgcgtc gccccattgg agcccgtccg cgccgggatg
     1261 aagcgcccag gcgggcaggg tggcgagcag cgcggccgca gccagcgtgg cgcggacgaa
     1321 tgagtgatgc agcatggcga tctccttgcg gcgcgtgtgc gattcccgcc gcctcggccc
     1381 gcgcctgccg tggcggcggt gctcctcgaa tcaggcggcg agcaactgcc tgagcacgaa
     1441 cgggaggatg ccgccgtgct tgtagtagtc gacctcgatc ggcgtgtcga tgcgcagcac
     1501 cagcggcacc gtgcgcgatc ggccgttcgt gtcggtgatg accagggtcg cgtcctgctg
     1561 cggcctgatc tcggggctca gttcgatgtc gaagcgctca tcgccgcgga tgccgagcga
     1621 ctcccacgag tccgcgccct tgaactgcag cggcagcacg cccatgccga ccaggttgct
     1681 gcggtggatg cgctcgaagc tgcgcgccac gaccgccttg acgccgagca gttgcgagcc
     1741 cttcgcggcc cagtcgcggc tcgagccggt gccgtactcc tcgccggcga agatcacggt
     1801 cggcgtgcct tcggcgatgt agcgcatcgc ggcgtcgtag atgaacatct tctcgcccga
     1861 cggttggaac agcgagacac cgccttcctc gcgcgagccg tcggcgctgg gcggaatcat
     1921 caggttcttg atgcgcacgt tcgcgaaggt gccgcgcatc atcacctcgt ggttgccgcg
     1981 gcgcgagccg taggagttga agtcggcctt gcccaccccg ttggcctcga gccagatgcc
     2041 ggcgggtgag gactgcttga tcgagccagc cggcgagatg tggtcggtcg tgatcgaatc
     2101 gccgaacagc gccatcgcgc gcgcgccttg cacgccggcg acgagctcgg ggggttccat
     2161 cgtgaagccg tcgaagaacg gcggcctggc gatgtaggtc gagcgcggcc agttgtagac
     2221 ctggcccgtc acgcccttga tcttttccca cagcttgccc ggctcggtcg cgaccttgtc
     2281 gtagttcgcc ttgaaggcct tgccgttcat cgcgtacttc atcagcgcgt ggacttcgtc
     2341 gctgctcggc cagatgtcgc cgaggtagat gtcgcgcccg cccttgcctt tgccgagcgg
     2401 ctcggtcatc gggtctttca gcacggtgcc ggcgatcgcg taggcgacga ccagcggcgg
     2461 gctggcgaga aagttcgcct tgatgttcgg gtgaatgcgc gcctcgaagt tgcggttgcc
     2521 cgacagcacg gccgcgcaga tcaggtcgtt ctgctgaatc gcttcgttga actcggggcg
     2581 caggtcgccg gcattgccga tgcaggtggt gcagccgtag gccgcgacat taaagccgag
     2641 cttctcgagg tagggcagca acttggcctt ctcgaggtat tcggtgacga ttcgcgaacc
     2701 cggggccagc gaggtcttga tgtgcg
//
LOCUS       DASRDG010000510         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1110843, whole genome shotgun sequence.
ACCESSION   DASRDG010000510 DASRDG010000000
VERSION     DASRDG010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110843"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /gene="hslV"
                     /locus_tag="VFA35_08575"
     CDS             complement(<1..404)
                     /gene="hslV"
                     /locus_tag="VFA35_08575"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZT56265.1"
                     /translation="MENYHGTTILSVRRGNVVALGGDGQVTLGTIVVKASARKVRKLY
                     RDQVLAGFAGATADAFTLFERFEAKLEKHQGHLVRAAIELTKDWRTDRVLRRLEAMLA
                     VADKETSLIISGNGDVLEPEQGIVAIGSGGGYA"
     gene            complement(465..2174)
                     /locus_tag="VFA35_08580"
     CDS             complement(465..2174)
                     /locus_tag="VFA35_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZT56266.1"
                     /translation="MSDPKDTTSFFRKVVKFVANPTTEWAELGAPTEDARETEYAKGE
                     LKAMIERKRRNDFVRKREFDMLRKVRREGLTSEQLAALGSSSRIDDSEGKMADTGVKA
                     DSGVKAKIDEIEQQMVGGDAAYAARRTPEFYNAPTEPAAMEMARARATSTRSGVNFDG
                     ARVEDFMSERAVPMATSPVVMGGGVSPLSAVTFDAAAPDLATPFAQEMTEIAHDPELD
                     EAVIAFANADFEQCEQALAALTGPGGARAQHAETWLVLFDLYRAIGQQHRFESLALDY
                     AQQFGWSAPQWFSLPRLVAEAASEDRPRRARVDGQVGWNSPEHIDAEAVAKLASVTLQ
                     MPLPWVFDWSALRSIDAEACARLSELFRSWIGQDLDMRWLGGERLFAVLAEAAPTGAR
                     DADPAYWQLRLDALRMTNRPDQFDEAAIDYCVTYEVSPPSWEPACCQVRISNPGATTT
                     SPPMSVVSDVSTSFVESRLTEDTAMVQVGTVELSGQLVGDIGPTLKKMDAELGPASIV
                     NVSCARLIRVDFIAAGDLLNWVLARRSENRSVRFVEAHRMVALFFGAMGINEHAKVKV
                     RIV"
BASE COUNT          313 a          779 c          789 g          378 t
ORIGIN      
        1 gcgtagccgc cgcccgagcc gatcgcgacg atgccctgct cgggctccag cacgtcgccg
       61 ttgccgctga tgatcagcga cgtctccttg tcggcgaccg cgagcatcgc ctcgagccgg
      121 cgcagcacgc ggtcggtgcg ccagtccttc gtcagctcga tcgcggcgcg caccaggtga
      181 ccctggtgct tctcgagctt ggcctcgaag cgctcgaaca gcgtgaacgc atcggcggtc
      241 gcgccggcga agccggccag cacctggtcg cggtagagct tgcgcacctt gcgggccgat
      301 gccttgacga cgatcgtgcc gagcgtgact tgcccgtcgc ccccgagcgc gaccacgttg
      361 ccgcggcgca cgctcaggat ggtcgtgccg tgatagtttt ccatcgaaag aagatagatg
      421 cgggccgggc tgtttcaagc gcggcggggt cagaaagaga tccgctagac gatgcggacc
      481 ttgactttgg cgtgctcgtt gatgcccatc gcgccgaaga acagcgccac catgcggtgc
      541 gcctcgacga agcgcacgct gcggttttcg ctgcggcgcg ccagcaccca gttcagcagg
      601 tcgccggcag cgatgaagtc gacccggatc agccgcgcgc acgagacgtt cacgatgctc
      661 gccgggccga gctcggcgtc catcttcttc agcgtcggcc cgatgtcgcc gaccaactgg
      721 ccggagagtt cgaccgtgcc gacctgcacc atggccgtgt cttcggtcag gcgcgattcg
      781 acgaagctgg tcgacacgtc gctgaccacc gacatcggcg gcgacgtggt cgtcgccccc
      841 gggttgctga tgcgcacctg gcagcacgcc ggctcccatg agggcggcga gacctcgtag
      901 gtcacgcaat agtcgatcgc ggcttcgtcg aattgatcgg gccggttggt catgcgcagc
      961 gcgtcgagtc gcagctgcca gtaggccggg tcggcatcgc gcgcgccggt cggcgcggct
     1021 tcggccagca cggcgaagag gcgttcgccg ccgagccagc gcatgtcgag gtcctggccg
     1081 atccagctgc ggaacagctc gctcaggcgc gcgcaggctt cggcatcgat gctgcgcagc
     1141 gcgctccagt cgaacaccca gggcagcggc atctgcagcg tgaccgacgc gagcttggcg
     1201 accgcctccg cgtcgatgtg ctcggggctg ttccagccga cctggccgtc gacacgcgcg
     1261 cggcgcggtc ggtcttcgct ggccgcctcg gccaccagcc tgggcagcga gaaccattgc
     1321 ggcgccgacc agccgaactg ctgcgcgtag tcgagtgcca ggctctcgaa cctgtgctgc
     1381 tggccgatgg cgcggtacag gtcgaacagc accagccagg tttcggcgtg ctgcgcacgc
     1441 gcgccgccgg ggccggtgag ggccgccagc gcctgctcgc actgctcgaa gtcggcattc
     1501 gcgaacgcga tcaccgcttc gtcgagctcg ggatcgtgcg cgatctcggt catctcttgc
     1561 gcgaacgggg tcgccaggtc gggcgcggcc gcatcgaacg tgaccgcgct gagcggcgag
     1621 acgccgccgc ccatcacgac cggcgaggtc gccatcggca cggcgcgctc gctcatgaag
     1681 tcttcgaccc gcgcgccgtc gaagttgacg ccggaccgcg tcgacgtggc gcgcgcgcgc
     1741 gccatctcca tcgcggccgg ctcggtcggc gcgttgtaga actcgggcgt gcggcgcgcg
     1801 gcatacgcgg cgtcgccgcc gaccatctgc tgctcgatct cgtcgatctt ggccttgacg
     1861 ccgctgtcgg ccttgacgcc ggtgtcggcc atcttgccct cggaatcgtc gatgcgcgag
     1921 gagctgccga gcgcggcgag ctgctcgctc gtcaggcctt cgcggcgcac cttgcgcagc
     1981 atgtcgaact cgcgtttgcg gacgaagtcg ttgcgccgct tgcgctcgat catcgccttc
     2041 agctcgccct tggcgtactc ggtctcgcgc gcgtcctcgg tgggcgcgcc gagctcggcc
     2101 cactccgtgg tcgggttggc gacgaacttg acaaccttgc ggaagaagct ggtcgtgtct
     2161 ttcggatccg acatggcgag gggctcgcgg gctccttggt tggatgaaag cgtttcggca
     2221 gcggtggcgt gagcggcgcg ggggccggtc agtccccga
//
LOCUS       DASRDG010000511         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1229953, whole genome shotgun sequence.
ACCESSION   DASRDG010000511 DASRDG010000000
VERSION     DASRDG010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229953"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFA35_08585"
     CDS             complement(<1..246)
                     /locus_tag="VFA35_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56267.1"
                     /translation="MAHEGTAPPRTPGLNNTWPGRTSTTWTGLPDHPPDSVLDSELSD
                     PNGHDAHVAARWIGWRLRLLVALALLGCLGIFALIRAL"
     gene            complement(269..1021)
                     /locus_tag="VFA35_08590"
     CDS             complement(269..1021)
                     /locus_tag="VFA35_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZT56268.1"
                     /translation="MPVKVLIVEDNPVARSFLSRVVRESFSDALVITEAGDLETARRQ
                     IGSLHGGAQAPDPFKLILVDLELPDGNGMELLAELGTYPATKIVTTLYSDDDHLFPAL
                     QCGADGYLLKEDRFEVLVEELQKIVRGQPPLSPAIARRLLTHFRHGPSAGGAATGSTP
                     NTGPVPLASSFQSSRPVPMGRGAPLDHERLTPRESEVLTYLSKGFTIKEIANLMGIKW
                     FTVNDHIKSIYKKLNVSSRAEAAVLASKQGLV"
     gene            complement(1098..2078)
                     /locus_tag="VFA35_08595"
     CDS             complement(1098..2078)
                     /locus_tag="VFA35_08595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HZT56269.1"
                     /translation="MKAWLCENPIGVEALVWKELPTPEPKAGEVRVAIRAASLNFPDI
                     LIVQNKYQMKPPLPFVPGSEYAGVVDAVGEGVTHLKLGDAVAAFGGTGGFGTHAIVKA
                     ALAMPLPPGFAFDDASAFVLTYATSHHALRDRAALQAGETVLILGAAGGVGTAAIQIA
                     KAIGAKVIAAASSDAKCELCKALGADATINYTTQNIRDEIKALTDGKGPDVVYDPVGG
                     DLAEPVFRSIGWRGRYLVVGFAQGGIPALPWNLALLKGASIVGVFWGEFARREPKANA
                     QALAELALWYAQGKIKPVIDQRLPMQQLPEAFAIMAGRQVRGKLVMTNEG"
     gene            2188..2943
                     /gene="surE"
                     /locus_tag="VFA35_08600"
     CDS             2188..2943
                     /gene="surE"
                     /locus_tag="VFA35_08600"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041929570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZT56270.1"
                     /translation="MRILIANDDGYLAPGLAALVKACEGLGDLDVVAPEQNASGTSNS
                     LTLGRPLSVHTASNGFLYINGTPSDCVHLALTGVLPNRPDLVVSGINNGANMGDDTLY
                     SGTVAAAMEGFLFGIPAIAFSQVQKGWGDLDAAARVARSLIEQVLKHPPLRAPYLLNV
                     NIPNRPDAAALPRVVTRLGRRHASEPLIAQTSPRGDTMYWIGPAGDAREAGPGTDFHA
                     TAGGQVSITPLQVDLTEHGALPEWSAWLGKAQA"
     gene            2940..3833
                     /locus_tag="VFA35_08605"
     CDS             2940..3833
                     /locus_tag="VFA35_08605"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZT56271.1"
                     /translation="MSGGDRRAPKFPLRLDALSSRNKVARTPVVPVAAPVLTTAPRLQ
                     ATAAAARAVLRPQRPLEHAHTEATRRGVPSGLGLDSADVRRRMAQRLRSAGVTNDRVI
                     EAMATVPRHLFVDAALVGQAYEDTSLPIGHGQTISKPSVVARMIELMLGGAGARASGS
                     LGRVLEIGTGCGYQAAVLALLAKQLVSIERLRPLHDKARELLAPLRPAHMRLVYGDGM
                     RGHAPNAPYDSIIAAAGGDALPQAWLDQLAIGGRLVSPVRQAGSQQQVLMVVDRTEQG
                     FEQNVYEAVHFVPLKSGTVAR"
     gene            3836..>4070
                     /locus_tag="VFA35_08610"
     CDS             3836..>4070
                     /locus_tag="VFA35_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56272.1"
                     /translation="MDKKYMLMNFKWTCVLLLAATVMAGCTSTPNRAPVEDRNAGMKV
                     PVAAGVPGEAAKPLPGAENSGKPGYYTVKPGDTL"
BASE COUNT          609 a         1434 c         1410 g          617 t
ORIGIN      
        1 cagcgcgcgg atcagcgcga agatgccgag gcagccgagc agcgccaagg ccaccagcag
       61 gcgcagccgc cagccgatcc agcgggccgc aacgtgggcg tcatggccgt tcgggtcgct
      121 caactccgag tcgagcacgg agtcgggcgg atgatccggc agccccgtcc aggtggtcga
      181 tgtgcggccg ggccaggtgt tgttcagtcc gggcgtgcgg ggcggggcgg tgccttcgtg
      241 cgccacggca gcaactcagg gtcgggctct agacgagccc ttgcttgctc gccagcacag
      301 ccgcttcggc gcggctcgac acgttgagct tcttgtagat cgacttgatg tggtcgttga
      361 cggtgaacca cttgatgccc atcaggttcg cgatctcctt gatcgtgaag cccttgctca
      421 ggtaggtgag cacctcgctc tcgcgcggcg tgaggcgctc gtggtcgagc ggcgcgccgc
      481 ggcccatcgg caccgggcgg ctgctctgga agctcgaggc cagcggcacc ggcccggtgt
      541 tcggggtgct gccggtggcc gcgccgcccg cgctcgggcc gtggcggaag tgcgtgagca
      601 agcgccgtgc gatcgccggc gagagcggcg gctggccgcg cacgatcttc tgcagctcct
      661 cgaccagcac ctcgaagcgg tcttccttca gcaggtagcc gtcggcgccg cactgcaggg
      721 ccgggaacag atggtcgtcg tcggagtaga gcgtcgtgac gatcttggtc gcgggataag
      781 tgccgagctc ggcgagcagt tccatgccgt tgccatcggg cagttcgagg tcgacgagga
      841 tcagcttgaa cgggtcgggc gcctgcgcgc ctccgtgcag cgagccgatc tggcggcgcg
      901 ccgtttccag gtcgccggcc tcggtgatga cgagtgcgtc actgaaactc tcgcgcacaa
      961 cccggctcag aaaactgcgc gcaaccgggt tgtcttcgac gatgagtacc ttgactggca
     1021 tctgttgcgc cctcgctctt gtgtgcggcg atggtagcgc aatcgggctg cggcaagccc
     1081 gctccggacc ctcagtctca gccctcgttc gtcatcacca gcttgccgcg cacctgccgg
     1141 ccggccatga tcgcgaaggc ctcgggcagc tgctgcatcg gcaggcgctg gtcgatcacc
     1201 ggcttgatct tgccttgcgc ataccacagc gccaactcgg ccagcgcctg cgcgttcgcc
     1261 ttcggctcgc ggcgcgcgaa ctcgccccag aacacgccga cgatcgaggc gcccttgagc
     1321 agcgccaggt tccacggcag cgccgggatg ccgccctgcg cgaagccgac gacgaggtag
     1381 cggccgcgcc acccgatcga acggaacacc ggctcggcca ggtcgccgcc caccgggtcg
     1441 tagaccacat ccggcccttt gccatcggtc agcgccttga tctcgtcgcg aatgttctgg
     1501 gtcgtgtagt tgatcgtcgc atcggcgccg agcgccttgc agagctcgca cttcgcgtcg
     1561 ctggatgcgg ccgcgatcac cttcgcgccg atcgccttcg cgatctggat cgccgcggtg
     1621 ccgacgccgc cggccgcgcc gaggatcagc acggtctcgc ccgcttgcag cgccgcgcgg
     1681 tcgcgcagcg cgtggtggct ggtcgcgtac gtcagcacga acgccgacgc atcgtcgaac
     1741 gcgaagcccg gcggcagcgg catcgcgagc gccgccttga cgatcgcatg ggtgccgaag
     1801 ccgccggtgc cgccgaacgc agcgaccgca tcgccgagct tcaggtgcgt gacgccttca
     1861 ccgaccgcat cgaccacgcc ggcgtactcg gagcccggca cgaacggcag cggcggcttc
     1921 atctggtact tgttctgcac gatcaggatg tcggggaagt tcaggctcgc agcgcggatc
     1981 gccacgcgca cctcgccggc cttcggctcg ggcgtcggca actccttcca caccagcgct
     2041 tcgacgccga tcgggttctc gcaaagccag gctttcatgg ggtctcctcg ggtgccgaat
     2101 ggccgcgagc gcggccattc ggcaaatgat acgcagcggc ccggatgcat cgcgtcgccg
     2161 acgtgacgtg tccatacaat cgaaaccatg cgcatcctga tcgccaacga cgacggttat
     2221 ctcgcccccg gcctcgccgc tctcgtgaag gcctgcgaag gcctcggcga tctcgacgtg
     2281 gtggcgcccg agcagaacgc cagcggcacc tcgaactcgc tgacgctggg gcggccgctg
     2341 agcgtgcaca cggcgagcaa cggctttctc tacatcaacg gcacgccgtc cgactgcgtc
     2401 cacctcgcgc tgaccggcgt gctgccgaac cggcccgatc tggtcgtctc gggcatcaac
     2461 aacggcgcca acatgggcga cgacaccttg tactcgggca ccgtcgcggc ggcgatggag
     2521 ggctttctgt tcggcatccc ggcgatcgcg ttctcgcagg tgcaaaaggg ctggggcgat
     2581 ctcgacgccg cggcgcgcgt ggcgcgcagc ctgatcgaac aggtgctgaa gcacccgccg
     2641 ctgcgcgcgc cgtacctgct caacgtcaac atcccgaacc ggcccgacgc cgctgcgctg
     2701 ccgcgcgtcg tcacaaggct cgggcgccgc cacgcgagcg agccgctgat cgcgcagacc
     2761 agcccgcgcg gcgacacgat gtactggatc ggcccggccg gcgacgcgcg cgaggccggc
     2821 ccgggcaccg acttccacgc caccgccggc ggccaggttt cgatcacgcc gctgcaggtc
     2881 gacctgaccg agcacggcgc cttgcccgag tggtcggcgt ggctcggcaa ggcccaggca
     2941 tgagcggcgg cgacaggcgc gcaccgaagt tcccgctgcg tctggatgcg ctgagctcgc
     3001 gcaacaaggt cgcgcgcacg cccgtggtgc cggtggccgc accggtgttg accaccgcgc
     3061 cgaggctgca ggcgaccgcc gcggcagcgc gcgcggtgct gcggccgcag cgcccgctcg
     3121 agcacgcgca caccgaggcc acgcggcgcg gcgtgccctc gggcctcggc ctcgactcgg
     3181 ccgatgtgcg ccgccgcatg gcgcagcgcc tgcgcagcgc cggcgtcacg aacgaccgcg
     3241 tgatcgaggc gatggcgacg gtgccgcgcc atctgttcgt cgatgccgcg ctcgtcggcc
     3301 aagcctatga agacaccagc ctgccgatcg gccacgggca gacgatctcg aagccttcgg
     3361 tcgtcgcgcg catgatcgag ctgatgctgg gtggcgccgg tgcgcgcgcc tcgggctcgc
     3421 tcggccgcgt gctcgagatc ggcaccggct gcggctacca ggctgcggtg ctggcgctgc
     3481 tcgcgaagca gctcgtgtcg atcgagcgcc tgcgcccgct gcacgacaag gcgcgcgaac
     3541 tgctcgcgcc gctgcggccg gcccacatgc gactggtgta tggtgacggc atgcgcggcc
     3601 acgccccgaa tgcgccctac gacagcatca tcgcggcggc cggcggcgat gcgctgccgc
     3661 aagcctggct cgatcagctc gcgatcggcg gccggctcgt gtcgccggtg cggcaggccg
     3721 gttcgcagca acaggtgctg atggtggtcg accgcaccga gcaggggttc gagcaaaacg
     3781 tgtacgaggc cgtgcacttc gtccctctaa aatcgggcac ggtcgcaaga tgacgatgga
     3841 caagaagtac atgctgatga atttcaaatg gacgtgcgtg ctgctgctcg ccgccaccgt
     3901 gatggcgggt tgcacgagca cgccgaaccg cgcgccggtc gaagaccgca atgccggcat
     3961 gaaggtgcct gtcgccgcgg gtgtgccggg cgaggccgcc aagcccctgc caggcgccga
     4021 gaacagcggc aagcccggct actacaccgt gaagccgggc gacacgctgt
//
LOCUS       DASRDG010000512         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1338516, whole genome shotgun sequence.
ACCESSION   DASRDG010000512 DASRDG010000000
VERSION     DASRDG010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338516"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFA35_08615"
     CDS             <1..234
                     /locus_tag="VFA35_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZT56273.1"
                     /translation="EGVNIYDAKTRLSQLVDRAAAGEDVIVSRNGKPLVRITQLTAPK
                     RRITFGVLKGKVKIAADFDAPLPDDVLAGFEGR"
     gene            234..611
                     /locus_tag="VFA35_08620"
     CDS             234..611
                     /locus_tag="VFA35_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZT56274.1"
                     /translation="MRLLLDTHIFLWAVAGSRLLKPAARRLIESADEVHVSAASIWEI
                     AIKARLGKVEAAPDELTAAIDASGFVQLPVSATHAAGVVRLPLHHNDPFDRLLIAQAL
                     AEPLRFVTADAALAPYTELVVLV"
     gene            complement(621..1019)
                     /gene="cueR"
                     /locus_tag="VFA35_08625"
     CDS             complement(621..1019)
                     /gene="cueR"
                     /locus_tag="VFA35_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HZT56275.1"
                     /translation="MNIGEASKHSGVSAKMIRHYESLHLLPAVARSESGYRQYDDTAV
                     HTLRFIRRARDLGFGTAEIAALLGLWRNRKRASSQVKRIALEHAADLQRRIEAMQAMQ
                     RTLEHLARGCAGDARPDCPILDDLAGGAHT"
     gene            complement(1016..>2553)
                     /locus_tag="VFA35_08630"
     CDS             complement(1016..>2553)
                     /locus_tag="VFA35_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZT56276.1"
                     /translation="AALRVGDTVVVRPGERLPADGLVQEGASHVDESLLTGESLPLPK
                     QTGDAVTGGAVNGEGLLVVRTTAVGAESALARIVRLVESAQAGKAPIQRLVDKISAVF
                     VPVVLGLALITLLGWGLATGDWSVGLIDAVSVLVIACPCALGLATPAAIMVGTGAAAR
                     LGILIKDAQALELAHSLRTVAFDKTGTLTAGRPTLVAAVPAPGSGLTREALIALAASL
                     QAGSEHPLARAVLAAAAAPVVPARELRALPGRGLAGRVGNRALQLGSARLMHELGIDG
                     AVPDAPSASLPRHAQAAPFQAEGRTVSWLVQAGAGERPQLLGLLAFGDAIKPGAAAAL
                     AQLQRLGLRTLMLSGDSRAAAEAVGRALGIDDVRAEVLPGDKAAVIAALKAHGAVAMV
                     GDGINDAPALAAADVGIAMAAGLPGQGGGSDVAMQAAGITLLRGDPALVARAIAISRM
                     THAKIRQNLFWALAYNVIGIPLAMLGLLSPVVAGAAMAASSVSVVGNALLLRRAIEKG
                     NAS"
BASE COUNT          346 a          952 c          910 g          345 t
ORIGIN      
        1 gaaggcgtga acatctacga tgccaagacc cggttgtcgc aactcgtcga tcgcgcggcc
       61 gcgggcgaag acgtgatcgt cagccgcaac ggcaagccgc tggtgcgcat cacgcaattg
      121 accgcaccga agcgccgcat cacgttcggc gtgctcaagg gcaaggtgaa gatcgcggcc
      181 gatttcgacg cgccgctgcc cgacgacgtg cttgcaggct tcgagggccg ctgatgcggc
      241 tgctgctcga tacgcacatc ttcctgtggg cggtggcggg ctcccggctg ctgaagccgg
      301 ccgcgcgccg cctgatcgaa tcggcagacg aggttcatgt gtctgccgcg tcgatctggg
      361 agatcgcgat caaggcgcgc ctcggcaagg tcgaggccgc gcccgatgaa ttgaccgcag
      421 cgatcgatgc cagcggtttc gtgcaactgc ccgtgagcgc cacgcatgcg gccggcgtgg
      481 tgcgcctgcc gctgcatcac aacgacccgt tcgatcggct gctgatcgcg caggcgctcg
      541 ccgagccgct caggttcgtg accgccgatg ccgcactcgc gccctacacc gaactcgtcg
      601 tgctggtttg acacgcgtgc tcaggtgtgc gcgccgcccg ccagatcgtc gaggatcggg
      661 caatcgggcc gcgcgtcgcc ggcgcagcca cgcgccaggt gctcgagcgt gcgctgcatc
      721 gcctgcatcg cttcgatgcg ccgctgcaga tcggccgcgt gttcgagcgc gatgcgcttg
      781 acctggctgc tggcgcgctt gcggttgcgc cacaacccga gcagggccgc gatctcggcg
      841 gtgccgaagc cgaggtcgcg ggcgcggcgg atgaagcgca gcgtgtgcac ggccgtgtcg
      901 tcgtactgcc ggtagccgct ctcgctgcgc gccaccgccg gcagcaggtg caagctctcg
      961 tagtggcgga tcatctttgc cgacacgccc gagtgcttcg aggcttcacc gatgttcatg
     1021 acgcgttgcc cttctcgatc gcgcgccgca gcagcagcgc attgccgacc acgctgacgc
     1081 tcgaggccgc catcgccgcg ccggcgacca ccgggctgag cagcccgagc atcgcgagcg
     1141 ggatgccgat cacgttgtag gcgagcgccc agaacaggtt ctggcggatc ttcgcgtgcg
     1201 tcatgcgcga gatcgcaatc gcgcgcgcga ccagcgccgg gtcgccgcgc agcagcgtga
     1261 tgcccgccgc ctgcatcgcc acatcgctgc cgccgccctg gccgggcagg ccggcggcca
     1321 tcgcgatgcc gacatcggcc gccgccagcg ccggcgcgtc gttgatgccg tcgccgacca
     1381 tcgcgactgc gccgtgcgcc ttcaacgctg cgatcaccgc ggccttgtcg ccgggcagca
     1441 cctcggcgcg cacgtcgtcg atgccgagcg cgcggccgac cgcctcggcc gcagcgcgcg
     1501 aatcgccgct gagcatcagc gtgcgcaggc ccaggcgctg gagctgcgcc agcgcggcgg
     1561 cggcgccggg cttgatcgcg tcgccgaacg cgagcaggcc cagcagttgc ggccgctcgc
     1621 ccgcgccggc ctgcacgagc cacgacacgg tgcggccctc ggcctgaaac ggcgcggcct
     1681 gcgcgtggcg tggcaacgac gcggacggcg cgtcgggcac ggcgccgtcg atgccgagtt
     1741 cgtgcatcag ccgtgcgctg ccgagctgca gcgcacggtt gccgacgcgc ccggccaggc
     1801 cgcgcccggg cagcgcgcgc agctcgcgcg cgggcacgac cggcgccgcg gcggcggcca
     1861 gcaccgcgcg cgccagcggg tgctcgctgc cggcctgcag gctcgccgcg agcgcgatca
     1921 gcgcctcgcg cgtcaggccc gagccgggcg caggcacggc ggcgaccagc gtcggccggc
     1981 ctgcggtcag cgtgccggtc ttgtcgaacg cgacggtgcg cagcgagtgg gccagctcca
     2041 gcgcctgcgc gtccttgatc aggatgccca ggcgcgccgc cgcgccggtg ccgaccatga
     2101 tcgcggccgg cgtcgcgagg ccgagcgcgc aggggcacgc gatcaccagc accgagaccg
     2161 cgtcgatcag gcccaccgac cagtcgccgg tcgcgaggcc ccagccgagc agcgtgatca
     2221 gtgcgaggcc cagcacgacc ggcacgaaca ccgcactgat cttgtcgacc agccgctgga
     2281 tcggcgcctt gcccgcctgc gcgctctcga ccagccgcac gatgcgcgcc agcgccgact
     2341 cggcgccgac cgccgtggtg cgcacgacca gcaggccttc gccgttgacc gcgccgcccg
     2401 ttaccgcgtc gcccgtctgc ttgggcagcg gcaggctctc gccggtgagc agcgattcgt
     2461 cgacgtggct cgcgccttcc tgcacgaggc cgtcggccgg aagccgctcg ccggggcgaa
     2521 cgaccactgt gtcgccgacc cgcagcgcgg cga
//
LOCUS       DASRDG010000513         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_191469, whole genome shotgun sequence.
ACCESSION   DASRDG010000513 DASRDG010000000
VERSION     DASRDG010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191469"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     repeat_region   7..340
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7..35
                     /rpt_unit_seq="gcgttccccacgtgcgtggggatgaaccg"
     gene            complement(345..602)
                     /locus_tag="VFA35_08635"
     CDS             complement(345..602)
                     /locus_tag="VFA35_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZT56277.1"
                     /translation="MTTWPEQDAKARFSELLDACLTDGPQMVTRRGVEAAVLMPAAQW
                     RRLQAATQPSLKDLLLSDDGRGELSVPSRWGARRRPVAAIG"
     gene            complement(611..1243)
                     /locus_tag="VFA35_08640"
     CDS             complement(611..1243)
                     /locus_tag="VFA35_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZT56278.1"
                     /translation="MIHASSLIGFALLSLGMVLTPGPNMVYVVSRSVCQGRRAGLVSL
                     GGVGLAFVVYMLAAALGITSLLMAVPLAYDTLRFAGAAYLGWLAWQALKPGGRSPFAV
                     QQLPADSPRRLFAMGFMTNLLNPKAALLYLSLLPQFIDPAAGSVFAQAVILGTMQIAI
                     SLSVNGLIALAAGSIAGFLGTRPLWLAVQRRLMGTMLAGLALRMALDARK"
     gene            1391..>2393
                     /locus_tag="VFA35_08645"
     CDS             1391..>2393
                     /locus_tag="VFA35_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZT56279.1"
                     /translation="MFHTLIRRLAGAAVVAVAGLLVGCGTLPSIDRAAIASEAIPLST
                     QTTLGRIAQGSMPAPELSGFRLMPLGLFSLDARVQLARRAEVSLDVQYYHFENDETGR
                     WLLRALRDAAERGVRVRLLIDDLYTGGQDPFWIAFAAHKNVQVRLFNPFVNARGRGQT
                     GRFIASAHEWSRVNHRMHNKLLIADGAMAVIGGRNVANEYYLRGAAQNYVDVDAFTVG
                     WIVPPLQFHFDRYWNSAAVYPLEAIAKTDLGAAELRARFEDWTGPVKTPPPQELPSTD
                     ILGYGPIADDLDGGRLGLIWGEAFVFADHPDKPFDGSVGGEPLDTSVTYNLFEVIRAA
                     "
BASE COUNT          381 a          856 c          820 g          336 t
ORIGIN      
        1 cgcgtcgcgt tccccacgtg cgtggggatg aaccggtgtg atcactaccg ccgtagtcga
       61 ccaggtggcg ttccccatgt gcgtggggat gaaccgatcg gcacgaactg ggagaacgag
      121 accgacgcgc gttccccacg tgcgtgggga tgaaccggag ttcatgaggt ttgcctttca
      181 atcgcgggcg gcgttcccca cgtgcgtggg gatgaaccga ttcgcgaccg cgcccgcgat
      241 ttgtcgcgca agcgttcccc acctgcgtgg ggatgaaccg ctcatgacca acacccgata
      301 agtccagtca agcgttcccc acgcgtccag caggcacctg ccgatcagcc gatcgccgcg
      361 acaggccgcc gacgtgcgcc ccagcgcgac ggcaccgaca attccccgcg cccgtcgtcc
      421 gacagcagca agtccttcaa cgagggctgg gtcgccgctt gcaggcgccg ccattgcgcg
      481 gcgggcatca gcaccgcggc ctcgacgccg cgccgcgtca ccatctgcgg cccgtcggtg
      541 aggcaggcgt cgaggagttc gctgaaccgc gccttcgcgt cctgttcggg ccaagtcgtc
      601 atgggttgtg ctacttccgc gcatccaacg ccatccgcag cgccagcccc gcgagcatcg
      661 tgcccatcag ccgccgctgc accgcgagcc acagcgggcg tgtgccgagg aagccggcga
      721 tcgagccggc ggcgagcgcg atcaggccgt tcacgctgag gctgatcgcg atctgcatcg
      781 tgcccaggat caccgcctgc gcgaacacgc tgccggccgc cgggtcgatg aactgcggca
      841 gcagcgagag atagagcagc gcggccttcg ggttgagcag gttggtcatg aagcccatcg
      901 cgaacaggcg gcgcgggctg tcggccggca gctgctgcac tgcgaacggc gagcgcccgc
      961 cgggcttcag cgcctgccac gcgagccagc cgaggtaggc cgcgccggcg aagcgcagcg
     1021 tgtcgtaggc cagcggcacc gccatcagca gcgaggtgat gcccagcgcc gcggcgagca
     1081 tgtagacgac gaacgcgagg ccgacgccgc cgagcgagac cagccccgcc cggcgcccct
     1141 ggcagaccga gcgcgagacg acgtagacca tgttcgggcc gggcgtgagc accatgccga
     1201 gcgagagcag cgcgaagccg atgagcgagg acgcgtggat catggccatc ccgaattggg
     1261 cgtgagaaga ccactcaact gtgccagaga caagcgcgtc tgcgcgtctg cgcgtctgcg
     1321 cgttttcgcg ggccgcgcag ccgtgcggcg tcatgcagaa tcgaccgcag ccgcacgcca
     1381 ggagacccgc atgttccaca ccttgattcg ccgcctcgcc ggtgcggcgg tcgtcgcggt
     1441 cgcggggctg ctcgtgggct gcggcacgct gccgtcgatc gaccgtgccg cgatcgccag
     1501 cgaggcgatc ccgctgagca cccagaccac gctcgggcgc atcgcgcagg gctcgatgcc
     1561 ggcgccggag ctctcgggct tcaggctcat gccgctgggc ctgttctcgc tcgacgcgcg
     1621 ggtgcagctc gcgcggcgcg ccgaggtctc gctcgacgtc cagtactacc acttcgagaa
     1681 cgacgagacc ggccgctggc tgctgcgtgc gctgcgcgac gcggccgagc gcggcgtgcg
     1741 cgtgcgcctg ctgatcgacg acctctacac cggcgggcag gacccgttct ggatcgcgtt
     1801 cgcggcgcac aagaacgtgc aggtgcgctt gttcaacccc ttcgtcaacg cgcgcgggcg
     1861 cggccagacc gggcgcttca tcgcctcggc gcacgagtgg tcgcgcgtca accaccgcat
     1921 gcacaacaag ctgctgatcg ccgacggcgc gatggcggtg atcggcgggc gcaacgtcgc
     1981 gaacgagtac tacctgcgcg gcgcggcgca gaactacgtc gacgtcgacg ccttcacggt
     2041 cggctggatc gtgccgccgc tgcagttcca cttcgaccgc tactggaaca gcgccgccgt
     2101 ctacccgctc gaagcgatcg cgaagactga cctgggcgcg gccgaactgc gcgcgcgctt
     2161 cgaggactgg accggcccgg tcaagacgcc gccgccgcag gagctgccct cgaccgacat
     2221 cctcggctat ggcccgatcg ccgacgacct cgacggcggc cgcctcggcc tgatctgggg
     2281 cgaggccttc gtgttcgccg accacccgga caagcccttc gacggcagcg tcggcggcga
     2341 accgctcgac accagcgtga cctacaacct gttcgaggtg atccgcgccg cga
//
LOCUS       DASRDG010000514         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1003520, whole genome shotgun sequence.
ACCESSION   DASRDG010000514 DASRDG010000000
VERSION     DASRDG010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003520"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2394
                     /gene="secA"
                     /locus_tag="VFA35_08650"
     CDS             <1..2394
                     /gene="secA"
                     /locus_tag="VFA35_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZT56280.1"
                     /translation="LNAITGQGVHVVTVNDYLARRDAEWMGRLYNFLGLTVGVNVPGM
                     SREEKQAAFAADVTYGTNNEFGFDYLRDNMVQDVADRVARGLAYAIVDEVDSILIDEA
                     RTPLIISGQAEDHTEMYVRINAVVPQLKKQIGELDLRTGEGVIEPGDFTVDEKSHQVF
                     LTEDGHENAERILGEAGLLAEGASLYDPANITLMHHVYAALRAKHLYNRDQHYVVQDG
                     EVIIVDEFTGRLMTGRRWSDGLHQAVEAKEGVQIQSENQTLASITFQNYFRMYGKLSG
                     MTGTADTEAYEFQEIYGLETVVIPPNKPTIRKDELDLVYKTSREKFEAVIKDIRDCHE
                     RGQPVLVGTTSIENSELISGLLQQAKLPHNVLNAKQHQREAEIVAQAGRPGVITIATN
                     MAGRGTDIVLGGNVENQIKVIDADPTIAEADKTVRAKQLHDEWQGLHEKVKAEGGLRI
                     IATERHESRRIDNQLRGRSGRQGDPGSSRFYLSLDDPLMRIFAGDRVRAIMERLKMPD
                     GEAIEAGIVTRSIESAQRKVEARNFDVRKQLLEYDDVSNDQRKVIYQQRNDILEAQSL
                     AEQIANLRGSTMTDVVRTYVPTDSVEEQWDLAGLEQVLADEWHVEVPLRAEVEKSASI
                     TDEEIVEKVTQAADAAFAEKVERVGLEQFTPFMRMILLQSIDSHWREHLAALDYLRQG
                     IHLRGYAQKNPKQEYKREAFELFSQLLDVVKMDVTRVLMTVRIQSQEEVAQAAEAIEA
                     SAERISNVTYTHPNEDGSVSQEADVATLAAEVPKVGRNDPCPCGSGKKYKHCHGRLA"
     gene            2516..>2784
                     /locus_tag="VFA35_08655"
     CDS             2516..>2784
                     /locus_tag="VFA35_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56281.1"
                     /translation="MTDSATTEASAQHELLRLALHNSARSVLLLVVAVAVIVAMGVHA
                     NRPVAAGVVSAVGLAVAVWRVLITRRHVRSPSGDEADLLRLQWEL"
BASE COUNT          541 a          904 c          948 g          391 t
ORIGIN      
        1 ctgaacgcaa tcaccgggca gggcgttcac gtcgtcacgg tcaacgacta cctcgcccgg
       61 cgcgatgccg agtggatggg ccggctgtac aatttcctcg gcctgacggt cggcgtcaac
      121 gtgcccggca tgagccgcga agaaaagcag gcggcgttcg ccgccgacgt gacctacggc
      181 accaacaacg agttcggctt cgactacctg cgcgacaaca tggtgcagga cgtggccgac
      241 cgcgtcgcgc gcggcctcgc gtacgcgatc gtcgacgagg tcgactcgat cctgatcgac
      301 gaggcgcgca cgccgctgat catcagcggt caggccgaag atcacaccga gatgtacgtg
      361 cgcatcaacg cggtcgtgcc gcagctcaag aagcagatcg gcgagctcga cctgcgcacc
      421 ggcgagggcg tgatcgagcc gggtgacttc acggtcgacg agaagagcca ccaggtcttc
      481 ctgaccgaag acgggcacga gaacgccgag cgcatcctcg gcgaagccgg gctgctggcc
      541 gaaggcgcga gcctgtacga cccggccaac atcacgctga tgcaccacgt gtacgccgcg
      601 ttgcgcgcca agcacctcta caaccgcgac cagcactacg tggtgcagga cggcgaggtg
      661 atcatcgtcg acgagttcac cggccggctg atgaccggcc gacgctggtc ggatgggctg
      721 caccaggcgg tcgaggcgaa ggagggcgtg cagatccaga gcgagaacca gacgctcgcg
      781 tcgatcacgt tccagaacta cttccgcatg tacggcaagc tctcgggcat gaccggcacc
      841 gccgacaccg aggcctacga gttccaggag atctacggtc tcgagacggt cgtgatcccg
      901 ccgaacaagc cgacgatccg taaggacgag ctcgacctgg tctacaagac cagccgcgag
      961 aagttcgagg cggtcatcaa ggacatccgc gactgccacg agcgcggcca gccggtcctg
     1021 gtgggcacga cctcgatcga gaactccgag ctgatctccg gcctgctgca gcaggcgaag
     1081 ctgccgcaca acgtgctgaa cgccaagcaa caccagcgtg aggccgagat cgtcgcgcag
     1141 gccgggcgcc cgggcgtgat cacgatcgcg accaacatgg ccggccgcgg caccgacatc
     1201 gtgctcggcg gcaacgtcga gaaccagatc aaggtcatcg acgcggatcc gaccatcgcc
     1261 gaggccgaca agacggtacg cgcgaagcaa ctgcacgacg agtggcaggg cttgcacgag
     1321 aaggtcaagg ccgagggcgg attgcgcatc atcgctacgg agcgccacga gtcgcgtcgc
     1381 atcgacaacc aactgcgcgg ccgctccgga cggcagggcg acccgggctc atcgcgcttc
     1441 tacctgtcgc tggacgaccc gctgatgcgc atcttcgccg gcgaccgcgt gcgcgcgatc
     1501 atggagcggc tgaagatgcc cgacggcgag gcgatcgagg ccggcatcgt cacgcgcagc
     1561 atcgagagcg cgcagcgcaa ggtcgaggcg cgcaacttcg acgtgcgcaa gcagctgctc
     1621 gaatacgacg acgtgagcaa cgaccagcgc aaggtgatct accagcagcg caacgacatc
     1681 ctcgaggcgc agagcctcgc cgagcagatc gcgaacctgc gcggaagcac gatgaccgac
     1741 gtggtgcgca cctatgttcc gaccgacagc gtcgaggaac agtgggatct cgctggcctc
     1801 gagcaggtgc tcgccgacga gtggcacgtg gaggtgccgt tgcgcgccga ggtcgagaag
     1861 agcgcgtcca tcaccgacga agagatcgtc gagaaggtca cgcaggctgc cgacgcggcg
     1921 ttcgccgaga aggtcgagcg tgtcggcctc gagcagttca cgccgttcat gcgcatgatc
     1981 ctgctgcagt cgatcgactc gcactggcgc gagcacttgg ccgcgctcga ctacctgcgc
     2041 cagggcatcc acctgcgcgg ctacgcccag aagaacccga agcaggagta caagcgcgag
     2101 gccttcgagc tgttctcgca gctgctcgac gtggtcaaga tggacgtgac gcgcgtgctg
     2161 atgaccgtgc gcatccagtc gcaggaagag gtcgcgcagg cggccgaggc gatcgaggcg
     2221 agcgccgagc gcatcagcaa cgtcacctac acccacccga acgaagacgg cagcgtgtcg
     2281 caagaggccg atgtggcgac gctcgccgcc gaggtgccga aggtcggccg caacgacccg
     2341 tgcccgtgcg gcagcggcaa gaagtacaag cactgtcacg ggcggctggc ctgacctcgc
     2401 tttcgcgctg cacgacacct ggccggtgtt gcatcggtgc gtacatgtgc cctcgttcgc
     2461 ccggccgtcg cgtgccgagc atgcccgcca cggtgtcggg cgttatgctt gacgcatgac
     2521 ggatagcgcg acgacagagg caagtgcgca gcacgaattg ctgcggcttg cgctgcacaa
     2581 ttcggcgcgc agcgtgctgt tgctggtggt ggcggtcgcg gtcatcgtgg cgatgggcgt
     2641 gcacgccaac cggcccgtcg cggctggcgt cgtgagtgca gtcggcctgg ctgtcgcggt
     2701 ctggcgggtg ctgatcacgc gtcgccatgt gcgcagcccg tcgggcgacg aagcagactt
     2761 gctgcgcttg cagtgggagc tcga
//
LOCUS       DASRDG010000515         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_309243, whole genome shotgun sequence.
ACCESSION   DASRDG010000515 DASRDG010000000
VERSION     DASRDG010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309243"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFA35_08660"
     CDS             complement(<1..489)
                     /locus_tag="VFA35_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HZT56282.1"
                     /translation="MAQRMQACTGCHGAQGRAASDGYYPRIAGKPAGYLYNQLVSFRD
                     GSRHYALMTNLLAPLSDAYLREIADHFAALDLPYPPPQPANAGVELLSRGETLVRRGD
                     AARQIPGCVQCHGAALMGVAPAIPALVGMPRDYLNAQLGAWRTGNRRAHAPDCMAQVA
                     KSL"
     gene            complement(620..2125)
                     /locus_tag="VFA35_08665"
     CDS             complement(620..2125)
                     /locus_tag="VFA35_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HZT56283.1"
                     /translation="MNADANTVPLLTPSQFRAAIAAVLTTILLAALDQTIVSVAVPTI
                     ARQLGGFEWMAWVISGYLIASTVVTPLYGRLSDLVGRRRVMTIAILIFLVASVACALA
                     QTLPQLVLARVVQGAGGGGLIATAQSVVADVVPLRERGRYQSYVSIVWAVASMLGPVI
                     GGVLTEYLSWPWIFWINLPVGAVALVLVHRSLKALPVTRRAVRIDGVGALLLLSGLTA
                     LLIPITRVGQGSAWSEAWNLTGWAVAAALLVVFVLQQRRHPDAIVPMALFGNRTVVAC
                     CALLFVCFFNFIAMSVLVPLRLQLSAGFSAADAALRLLPLTLAIPAAAFASGRWLSRT
                     GRVLPAQRLGVALVPLALIAMGFGQPTGALATVALIVLGAGMGLQMPTTLITVQQSVP
                     RAQIGTVTALTSFFRLLGGAIGIAVLSSVVLSLLRGQLPAGVTALDAEALGRMLDAAR
                     GGHAPAGDAAFQQVMWLSAAVATVSLWLVARLPDLRLHDAPGAAQEAAAEL"
     gene            complement(2122..2763)
                     /locus_tag="VFA35_08670"
     CDS             complement(2122..2763)
                     /locus_tag="VFA35_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZT56284.1"
                     /translation="MYLPTHFEEKRPELLHALLRAHPLGLLVTQDAAGEIAADALPFI
                     LDADPAGGPGVLRAHVARANPLWRAARGDIDSLVVFQGEHAYVSPSFYPSKAEHGKVV
                     PTWNYVMVQARGRLRAVDDADWLRAFVTRLTERHEAPRAAPWAVGDAPADFITTMLGA
                     IVGIEIVLTALTGKWKVSQNRSAADRAGVARGLADAGIRFEACSAAPPVADRS"
     gene            3018..4370
                     /locus_tag="VFA35_08675"
     CDS             3018..4370
                     /locus_tag="VFA35_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZT56285.1"
                     /translation="MDERRLLCVRTKYSDCAWIEQLEAEGWGVEVAGNLIAAHRQLQE
                     RPYLAGLIVPGGIDAVACAEFDAFLRTHGGLEWVGAFEPAVLALPACRDLIVDHLFDH
                     HTIPVNPAKLASTLGHAHGHAALRQASRAAQVLTPAPDTSIVGNSAAARQLLRQIMRV
                     AKVDAPVLVCGESGSGKELTAQAIHRHSSRANGPFVPVNCGAIQATLIQSELFGHEKG
                     AFTGASKEGRGLIEAANGGTIFLDEIGDLSLELQINLLRFLQEKTINRVGSTRSIRVD
                     ARVIAATHVDLEKAVATGTFREDLFYRLNVVPLRVPALRERLTDVGPLAEHFFQQFVA
                     EKGPQLKGFSRRAMVALGEHQWPGNVRELINRVRRAMVMAEGRLIGPADLGFVERDES
                     RAWDALEEARTRAERGAISVSLQQAGRNVTEAARQLGVSRMTLYRLMAKHGIDHDLRN
                     "
     gene            4738..>5229
                     /locus_tag="VFA35_08680"
     CDS             4738..>5229
                     /locus_tag="VFA35_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56286.1"
                     /translation="MKKTILATAITLAFGTAWANPHNESNKANGSNTQNATATSTQTG
                     AGPNANENSTATLTNDSSNNSRTKTNNSNNSDRNNNKSSGDNRDNKGKAKADGYGTTA
                     ANNGSSATANFSNAFNTNKAVAVSKLEGSVSGIKVWGLGNSAINNGNADGGKGGTGNG
                     GKGQ"
BASE COUNT          905 a         1818 c         1830 g          676 t
ORIGIN      
        1 caggctcttg gcgacctgcg ccatgcagtc gggcgcgtgc gcccgccggt tgccggtgcg
       61 ccaggcgccg agttgcgcgt tgaggtagtc gcgcggcatg ccgacgagcg ccggaatcgc
      121 tggcgcgacg cccatcagcg ccgcgccgtg gcactgcacg cagcccggga tctgccgcgc
      181 cgcatcgccg cgccgcacca gcgtttcgcc gcggctcagc agctcgacgc cggcgttcgc
      241 gggttggggc ggcgggtagg gcaggtcgag cgccgcgaag tggtcggcga tctcgcgcag
      301 gtaggcgtcg gagagcggcg cgagcaggtt ggtcatcagc gcgtagtggc gactgccgtc
      361 gcgaaaactc acgagctggt tgtagaggta gccggccggc ttgcccgcga tgcgcgggta
      421 gtagccgtcg ctcgcggccc ggccctgcgc gccgtggcag ccggtgcagg cctgcatgcg
      481 ctgggccatc gtgtcttcga ccctcggcgc agcgtgaacc agcgccgccg tgctgatcag
      541 cagggcagcg atcaccgcgt cgcaaggtct tgcagaacga gagcgcatgg acatgcgcgg
      601 gttgtaccaa acgcgccggt cacagctccg cggccgcctc ctgcgccgca cccggcgcgt
      661 cgtgcaggcg caggtcgggc agccgcgcga ccagccacag cgaaaccgtg gccaccgccg
      721 cgctcagcca catcacttgc tggaacgccg cgtcgccggc cggcgcgtgc ccgccgcgcg
      781 cggcatcgag catgcggccg agcgcttcgg cgtcgagtgc ggtcacgccg gccggcagct
      841 gcccgcgcag cagcgacagc acgaccgagc tcagcaccgc gatgccgatc gcgccgccga
      901 gcaggcggaa gaacgaggtc agcgcggtca cggtgccgat ctgcgcgcgc ggcaccgact
      961 gctgcaccgt gatcagcgtc gtcggcatct gcaggcccat gccggcgccg agcacgatca
     1021 gcgcgaccgt ggcgagcgcg ccggtcggct ggccgaaacc cattgcgatc agcgcgagcg
     1081 gcaccagcgc cacgccgagc cgctgcgccg gcaggacccg gccggtgcgg ctcagccagc
     1141 ggccgctcgc gaacgccgcg gcggggatcg cgagcgtcag cggcagcagc cgcaacgcgg
     1201 cgtcggccgc actgaagccg gcgctcagct gcaggcgcaa cggcaccaac accgacatcg
     1261 cgatgaagtt gaagaagcag acgaacagca gcgcgcagca cgcgacgacc gtgcggttgc
     1321 cgaacagcgc catcggcacg atcgcgtccg gatggcgccg ctgctgcaac acgaacacga
     1381 cgagcagcgc cgcggcgacc gcccagccgg tcaggttcca cgcctcgctc catgcgctgc
     1441 cctggccgac gcgcgtgatc gggatcagca acgcggtgag cccgctgagc agcagcagcg
     1501 cgccgacgcc gtcgatgcgc accgcgcgcc gcgtcacggg cagggccttc agcgagcgat
     1561 gcaccagcac cagcgcgacc gcgccgaccg gcaggttgat ccagaagatc cacggccacg
     1621 acaggtattc ggtcagcacg ccgccgatca ccggcccgag catgctcgcc accgcccaga
     1681 cgatgctgac atagctctgg tagcgcccgc gctcgcgcag cgggaccaca tcggcgacga
     1741 ccgactgcgc ggtcgcgatc agcccgccgc cgcccgcgcc ctggacgacg cgcgcgagca
     1801 cgagctgcgg cagcgtctgc gcgagcgcgc acgcgaccga ggcgacgagg aagatcagga
     1861 tcgcgatggt catcacgcgg cgccggccga ccagatcgct cagccggccg tacagcggcg
     1921 tgaccacggt cgaggcgatc aggtagcccg agatcaccca agccatccac tcgaagccgc
     1981 cgagctggcg cgcgatcgtc ggcaccgcga cgctgacgat ggtctggtcg agcgccgcga
     2041 gcaggatcgt cgtcagcacc gcggcgatcg cggcgcggaa ctggctcggg gtcagcagcg
     2101 ggacggtgtt cgcgtcggcg ttcaagatcg atccgcgacg ggtggggctg ccgagcatgc
     2161 ctcgaatcga atgcctgcgt cggccaggcc gcgcgccacg ccggcgcggt cggcggccga
     2221 tcggttctgg ctcaccttcc acttgccggt gagcgcggtg aggacgatct cgatgccgac
     2281 gatcgcgccg agcatcgtcg tgatgaagtc ggcgggcgcg tcgcccacgg cccagggcgc
     2341 ggcgcgcggc gcctcgtgcc gctcggtcag gcgcgtcacg aaggcgcgca gccagtcggc
     2401 atcgtcgacc gcgcgcagcc ggccacgcgc ctgcaccatc acgtaattcc aggtcggcac
     2461 gaccttgccg tgctcggctt tgctcgggta gaagctcggc gagacatagg catgctcgcc
     2521 ctggaatacg acgagcgagt cgatgtcgcc gcgcgccgcg cgccacagcg ggttcgcgcg
     2581 cgcgacgtgc gcgcgcagga cgccggggcc gccggccggg tcggcatcga ggatgaaggg
     2641 cagggcgtcg gcggcgatct cgcccgcggc gtcttgcgtg acgagcaggc cgagcgggtg
     2701 cgcgcgcaac agcgcgtgca gcagctcggg gcgcttctct tcgaaatggg tgggtaggta
     2761 catgggtcga ttcacgatga cgacggcgcg tgcgcggcgc ggcttctccg cgtcgatgtt
     2821 gccatggcgc gcgccggtcg acccgcgccc cccaaacaca tggggtggca tgccccgggg
     2881 gcgggggcta ccgttgtctt cgcttgcggt tgacgggcag tgccgggcat tttgaccggt
     2941 gcacccgtgg gttcgaaagc gcgacgcggc cttgctgttc cgccccgccg gcgcaacaag
     3001 aacgggtcgg agacaacatg gacgaacggc gcctcctgtg cgtcaggacg aagtactcgg
     3061 actgcgcgtg gatcgaacaa ctggaagccg agggctgggg cgtcgaggtt gcgggcaacc
     3121 tgatcgccgc gcaccggcag cttcaggagc ggccgtacct ggcagggctg atcgtgcctg
     3181 gtgggatcga tgcggtggcc tgcgccgagt tcgacgcgtt cctgcgcacg cacggcgggc
     3241 tcgagtgggt cggcgcgttc gagcccgcgg tgctcgcgct gccggcgtgc cgcgacctga
     3301 tcgtcgatca cctgtttgat caccacacga ttccggtcaa tccggccaag ctggcgtcga
     3361 cgctcggcca tgcgcacggc cacgccgcgc tgcgccaggc ctcgcgtgcg gcccaggtgc
     3421 tgacgccggc gcccgacacc tcgatcgtcg gcaacagcgc ggcggcgcgg cagctgctgc
     3481 gccagatcat gcgcgtcgcg aaggtcgatg cgccggtgct cgtctgtggc gagagcggca
     3541 gcggcaagga gttgacggcg caggcgatcc accgccattc gagccgtgcc aacgggccat
     3601 tcgtgccggt gaactgcggg gcgatccagg ccacgctgat tcagtcagag ctgttcgggc
     3661 acgagaaggg cgcgttcacc ggcgcctcga aggaagggcg cggcctgatc gaggccgcca
     3721 acggcggcac gatcttcctc gacgagatcg gtgatctctc gctcgagctg cagatcaacc
     3781 tgctgcgctt cctgcaggag aagacgatca accgcgtcgg ctcgacgcgc agcatccgcg
     3841 tcgacgcacg cgtgatcgcg gccacccacg tcgacctcga aaaggcggtg gccacgggca
     3901 cgtttcgcga agacctgttc taccgcctca acgtcgtgcc gctgcgcgtg ccggcgctgc
     3961 gcgagcggct caccgacgtg gggccgctgg ccgagcactt cttccagcag ttcgtggccg
     4021 agaaggggcc gcagctgaag ggcttcagcc gccgtgcgat ggttgcgctc ggcgagcacc
     4081 aatggccggg caacgtgcgt gagctgatca accgcgtgcg gcgtgcgatg gtgatggccg
     4141 agggccggct gatcggaccg gccgacctcg gcttcgtcga acgcgacgag tcgcgcgcct
     4201 gggacgcgct cgaagaagcg cgcacgcgcg ccgaacgcgg cgcgatctcg gtcagcctgc
     4261 aacaggccgg ccgcaacgtg accgaggcgg cgcggcaact cggcgtctcg cgcatgacgc
     4321 tgtaccgcct gatggccaag cacgggatcg accacgacct gcgcaattga gcccgcggcc
     4381 tacccgcctc ggcccgatgc gggcaaaccc ccgtaacaaa agcgaacagc ccccgaagat
     4441 gcgggggagc ttcgccaagc cgttgatttt caacaggaaa ttctgctttc gtcaaaaaga
     4501 cccccgccta gcgccgaaac ggccggcgcg gtgtcgcggc aacgtgacag tcagcgaccg
     4561 aaacagccct gcaagggccc ctgtcagcgt cgcatcgcag cggacttctt caatcaattc
     4621 aagcacttgg gcacatccgg cggcaattgg tcccgaatct gcaacttgat gtgcgaattg
     4681 gcacgacgcc agttcccact gcttcgcacg gaccaaccct ctctcaggag ccttcaaatg
     4741 aagaaaacca tactggcaac cgccatcacc ctcgccttcg gtactgcgtg ggcgaacccg
     4801 cacaacgaaa gcaacaaggc gaacgggtcc aacacccaga acgccacggc aacctcgacg
     4861 cagacgggcg ctggcccgaa cgccaacgag aattcgacgg ccacgctgac gaacgacagc
     4921 tcgaacaatt cgaggacgaa gaccaacaac tccaacaact cggaccgcaa taacaacaag
     4981 agtagcggcg acaaccgtga caacaagggc aaggccaagg ccgacggcta cggcaccact
     5041 gcggccaaca acggcagcag cgccaccgcc aatttcagca acgccttcaa caccaacaag
     5101 gcggttgcgg tgagcaagct ggaaggctcg gtcagcggca tcaaggtctg gggcctgggc
     5161 aacagcgcca tcaacaacgg caatgccgat ggcggcaagg gcggcacggg caacggcggc
     5221 aagggccag
//
LOCUS       DASRDG010000516         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_533078, whole genome shotgun sequence.
ACCESSION   DASRDG010000516 DASRDG010000000
VERSION     DASRDG010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533078"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2037)
                     /locus_tag="VFA35_08685"
     CDS             complement(<1..>2037)
                     /locus_tag="VFA35_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT56287.1"
                     /translation="GAKDYGFVGRVNGVPATLIGIFLQPGSNALQVGRSVRELLARLA
                     TAFPVGLRHDITYDTTSFVEVSIIEVLKTLGIAALLVFAVVFVFLQSWRATLIPALAV
                     PVSLIGSFAGLHLLGYSINTLTLFGMVLAIGIVVDDAIVVLENVERLMHERRLHPHAA
                     AVEAMREVTGPVIAIVLVLIAAFVPIAFLGGLTGILFRQFAVTIAIAVTISGFVALTL
                     TPALCAVLLKNQPRGRNWFFRAFDRVFEHTAHGYAGGVRWLARHALVGVALFLVMVAG
                     TALLWKITPGSLVPDEDQGFYIAAVYLPDGSSLQRTDEVVKQVSDIVLSNKANENCTA
                     FAGFDFLGGGFKANAATMFITQRHWDERPGVDTKQLVGELFMKTAGLRDALVIAIGPP
                     PILGLGTAGGCEVWLQNQGEGGPQRMAEIVGAFQAAAVTVPPGATKPPLASAQTLWRA
                     FAPQLHIDIDRDRATELGVAIDDAFDTLSAALGTVYVNDFNKYGRNWQVLLSAEPAQR
                     DKPADVEAVWVRSRNGAMVPASAFVHVRYTSGPESLDRFDNHPAVHLFCAGEPGMSSG
                     ETIAAVEKLARTVLPPDFSYAWYGASFQEKRVGSASAMALAMAALMVFLILAAQYERW
                     SLPFAVLLALPFGTFGALAAVNLRGFTNDVYFQIGLVTLLGLAAKNAILIVQFA"
BASE COUNT          333 a          710 c          721 g          273 t
ORIGIN      
        1 tcggcgaact gcacgatcag gatggcattc ttcgccgcca ggccgagcag cgtgacgagg
       61 ccgatctgga agtagacgtc gttggtgaag ccgcgcaggt tgactgcggc gagcgcgccg
      121 aaggtgccga acggcagcgc cagcagcacc gcgaacggca gcgaccagcg ctcgtactgc
      181 gcggcgagga tcaggaacac catcagcgcc gccatcgcca gcgccatcgc gctggcgctg
      241 ccgacccgct tctcctggaa cgacgcgccg taccaggcgt acgagaagtc gggcggcagc
      301 acggtcctgg cgagcttctc gaccgccgcg atcgtctcgc ccgagctcat gcccggctcg
      361 cccgcgcaga acaggtgcac cgccgggtgg ttgtcgaagc ggtcgagcga ctcggggccg
      421 gacgtgtagc gcacgtgcac gaacgcgctc gcgggcacca tcgcgccgtt gcgcgaacgg
      481 acccacaccg cctcgacgtc ggcgggcttg tcgcgctgcg cgggctcggc cgacagcagc
      541 acctgccagt tgcggccgta cttgttgaag tcgttgacgt agacggtgcc gagcgccgcc
      601 gacagggtgt cgaacgcgtc gtcgatcgcg acgccgagct cggtggcgcg gtcgcggtcg
      661 atgtcgatgt gcagctgcgg cgcgaacgcg cgccacagcg tctgcgcgct cgcgagcggc
      721 ggcttcgtgg cacccggcgg caccgtgacc gcggcggcct ggaacgcgcc cacgatttcg
      781 gccatgcgct gcggcccgcc ctcgccctgg ttctgcagcc acacctcgca gccgccggcg
      841 gtgccgaggc cgaggatcgg cggcgggccg atcgcgatca ccagcgcgtc gcgcaggccg
      901 gcggtcttca tgaacagctc gccgaccagc tgcttggtgt cgacgccggg acgttcgtcc
      961 cagtgcctct gcgtgatgaa catcgtcgcg gcgttggcct tgaagccgcc gccgaggaag
     1021 tcgaagcccg cgaacgccgt gcagttctcg ttggccttgt tgctcagcac gatgtcgctg
     1081 acctgcttca ccacctcgtc ggtgcgctgc agcgagctgc cgtcgggcag gtagaccgcg
     1141 gcgatgtaga agccctggtc ctcgtcgggc acgagcgagc cgggggtgat cttccacagc
     1201 agggcggtgc ccgcgaccat caccaggaac agggcgacgc cgaccagcgc gtggcgcgcg
     1261 agccagcgca cgccgcccgc gtagccgtgc gcggtgtgct cgaacacgcg gtcgaaggcg
     1321 cggaagaacc agttccggcc gcgcggctgg ttcttcagca gcacggcgca cagcgccggc
     1381 gtcagcgtga gcgcgacgaa acccgagatc gtgaccgcga tcgcgatggt gaccgcgaac
     1441 tggcggaaca ggatgccggt gagcccgccg aggaacgcga tcggcacgaa cgcggcgatc
     1501 agcaccagca cgatcgcgat caccggcccg gtgacctcgc gcatcgcctc gaccgcggcc
     1561 gcgtgcgggt gcaggcgccg ttcgtgcatc agccgctcga cgttctcgag cacgacgatc
     1621 gcgtcgtcga cgacgatgcc gatcgccagc accatgccga agagcgtcag cgtgttgatc
     1681 gagtagccga gcaggtgcag gcccgcgaac gagccgatca gcgacacggg caccgcgagc
     1741 gccgggatca gcgtcgcgcg ccagctctgc aggaacacga acaccaccgc gaacaccagc
     1801 agcgccgcga tccccagcgt cttcaggacc tcgatgatcg acacctcgac gaagctcgtg
     1861 gtgtcgtagg tgatgtcgtg acgcaggccg acggggaacg ccgttgccag ccgcgcgagc
     1921 agctcgcgca ccgagcggcc gacctgcagc gcgttggagc cgggctgcag gaagatgccg
     1981 atcagcgtcg cgggcacgcc gttgacgcgg ccgacgaacc cgtagtcctt ggcgccg
//
LOCUS       DASRDG010000517         4795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_438607, whole genome shotgun sequence.
ACCESSION   DASRDG010000517 DASRDG010000000
VERSION     DASRDG010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438607"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /gene="dtd"
                     /locus_tag="VFA35_08690"
     CDS             complement(<1..394)
                     /gene="dtd"
                     /locus_tag="VFA35_08690"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZT56288.1"
                     /translation="MIALIQRVARAEVQAAGASIGAIGAGLLVLVCAEPDDTDAIADK
                     LVAKLLKLRIFSDHAGKMNLSVADVNGALLIVSQFTLAADVSGGNRPSFTAAAGPVLG
                     RRLYERVVATARALHPQVACGEFGAEMQV"
     gene            complement(391..1281)
                     /locus_tag="VFA35_08695"
     CDS             complement(391..1281)
                     /locus_tag="VFA35_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZT56289.1"
                     /translation="MQVKTLLKLSIAAAVATIAMKSAAWWITDSVGLLSDAMESFVNL
                     ASALFAIAMVTIAQRPADADHPFGHTKAEYFSSGFEGLLIVGAAAGIVWAAVHRFAAP
                     QALQSLGWGMGLAVLSSALNGALAWRMMAAAREHRSIALEADARHLYTDVWTSAGVVV
                     GLVLVHFTGWLWLDPVLAILVALNILREGAHLAWRSVEGLMDSAVEPEVRAQIDATLA
                     RFARREIRFDHIVTRRAAQRRFVDLHMHMPAHWSLGQAATLRAEVEKALMNEVPGLRA
                     SIQLLPSNVEAHADDLQAAP"
     gene            complement(1298..1645)
                     /locus_tag="VFA35_08700"
     CDS             complement(1298..1645)
                     /locus_tag="VFA35_08700"
                     /inference="COORDINATES: protein motif:HMM:NF036460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HZT56290.1"
                     /translation="MSLSALMALRAAAAAPPPHEQARIEKLIRFVEGQRDIKFIRNGT
                     EYSSAEAGKFLRGKLEAMGQEVNTAREFIERIASRSSMSGKPYEVKFADGRMMLASQF
                     LGAELKRLEGQPV"
     gene            complement(1686..2945)
                     /gene="tyrS"
                     /locus_tag="VFA35_08705"
     CDS             complement(1686..2945)
                     /gene="tyrS"
                     /locus_tag="VFA35_08705"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZT56291.1"
                     /translation="MSQPSSQASAAAPSGPVHPVTDRVEAAMAISLRGCDELLPQAEW
                     LAKLARSEATGVPLRIKFGLDPTAPDLHLGHTVVFNKLRQLQDLGHTVIPLIGDFTSM
                     IGDPSGRNTTRPPLTREQIEANANTYFDQISLVLDIERAEVRYNSEWSDPLGARGMIQ
                     LASRYTVARMMERNDFHARFKAGTPISVHEFLYPLMQGYDSVALRSDIELGGTDQKFN
                     LLVGRALQAEYGQEPQCILTMPLLEGLDGVEKMSKSKGNYIAITEPANDMFAKLLSIS
                     DVLMWKYFTLLSFRPEAEIARFKAEIAAGRNPKDVKVLLAKEITTRFHGAAAADAAEL
                     DFEHRARGGVPDVIDAVTLAGAPLGIGALLKQANLAASTGEGLRLVDGGGVRVDGAVV
                     GDRGLKLGAGTYVVQVGKRKFARVTLE"
     gene            3180..4565
                     /locus_tag="VFA35_08710"
     CDS             3180..4565
                     /locus_tag="VFA35_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZT56292.1"
                     /translation="MLDLLDRAVASATAQTGRFVSAHPRSLSAAVMLGCAGFGVTAFG
                     IAPMAPDAADIPKRLVTETVTPDGIQSQLDALAEHELELYRTDLTRASDTVDSLLKRL
                     NVSDAGAAQFLRSDTTARKLLEGRGGKMVRVQTDENGVLTELVARYAPADPEQLGTYF
                     TRLTVKRIAGKLLAMVETAPLASQVRMGSGTIRSSLFAATDEARIPDGVATQLAEVFA
                     TDLDFHRQLQAGDTFSVLYEALTADGEPITWSGSAAGKVLAADFVNNGKTYSAIWFKG
                     ADGKGGYYGFDGHSKKRAFLASPMEFSRVTSGFAMRMHPILNSWKQHKGVDYGAPSGT
                     AIRSVGDGTVDFAGWQNGYGNVVEIRHSAQRSTLYAHMSRIDVKRGAHIDQGQRIGAV
                     GATGWATGPHLHFEVKVDGVQQNPLIMAKASETIPLASAQKAEFAQLAKRLKGQLEVA
                     DSLARSGAYAE"
     gene            4635..>4795
                     /locus_tag="VFA35_08715"
     CDS             4635..>4795
                     /locus_tag="VFA35_08715"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HZT56293.1"
                     /translation="MTERYIGLMSGTSLDGVDGVLVDFDAAPMRVVAHAHRAFEPALA
                     HELLALNAPG"
BASE COUNT          769 a         1666 c         1633 g          727 t
ORIGIN      
        1 tgacctgcat ctcggcgccg aactcgccgc acgcaacctg cgggtgcagc gcgcgcgcgg
       61 tcgcgacgac gcgctcgtag agccggcggc cgagcacggg cccggcggcg gcggtgaagc
      121 tcggtcggtt gccgcccgag acatcggccg ccagcgtgaa ctggctgacg atcagcaggg
      181 cgccgttcac gtcggcgacg ctgaggttca tcttgccggc gtggtccgag aaaatgcgca
      241 gcttcagcag cttggcgacg agcttgtcgg cgatcgcatc ggtgtcgtcg ggctcggcgc
      301 agaccagcac cagcaggccg gcgccgatcg caccgatcga agcgcccgcc gcctgcacct
      361 ccgcgcgcgc gacgcgctgg atcaacgcga tcacggcgcc gcctgcaggt cgtcggcgtg
      421 ggcttcgacg ttcgacggta gcagctggat cgaggcgcgc agccccggca cctcgttcat
      481 cagcgccttc tcgacctcgg cgcgcagcgt cgccgcctgg cccagcgacc agtgcgccgg
      541 catgtgcatg tgcaggtcga cgaagcggcg ctgtgcggcg cgccgcgtca cgatgtggtc
      601 gaagcggatc tcgcggcgcg cgaaccgagc gagcgtcgcg tcgatctgcg cgcgcacctc
      661 gggctcgacc gcgctgtcca tcagaccctc gaccgagcgc cacgccaggt gcgcgccttc
      721 gcgcaggatg ttcagcgcaa cgaggatcgc gagcaccggg tcgagccaga gccagccggt
      781 gaagtggacg aggacgagcc cgacgacgac gcctgcggag gtccagacgt cggtgtacag
      841 atggcgtgca tcggcctcga gcgcgatcga gcggtgctcg cgcgcggcgg ccatcatgcg
      901 ccaggcgagc gccccgttca gcgccgagct gagcacggcc aggcccatgc cccagcccag
      961 cgactgcagc gcttgcggcg cggcgaagcg gtgcaccgcg gcccagacga tgccggcggc
     1021 agcgccgacg atcagcagcc cttcgaagcc gctcgagaag tactcggcct tggtgtggcc
     1081 gaacggatga tccgcatcgg cggggcgctg cgcgatcgtc accatcgcga tcgcgaacag
     1141 cgccgaagcg aggttgacga aggactccat cgcgtcggag agcaggccga ccgaatcggt
     1201 gatccaccac gccgccgact tcatcgcgat cgtcgcgacg gcggccgcga tcgagagctt
     1261 gagaagggtc ttgacctgca ctcggcgcag ccagaaccta gaccggctgg ccttcgaggc
     1321 gcttcagctc cgcgccgagg aactggctcg ccagcatcat ccggccgtcg gcgaacttca
     1381 cctcgtaggg cttgccgctc atgctcgacc ggctcgcgat gcgctcgatg aactcgcgcg
     1441 cggtgttcac ctcctggccc atcgcctcga gcttgccgcg caggaacttg ccggcctcgg
     1501 cactgctgta ctcggtgccg ttgcggatga actttatgtc cctctggcct tcgacgaagc
     1561 ggatcagctt ctcgatgcgc gcctgctcgt gcggcggcgg tgcggccgcc gcggcgcgca
     1621 gcgccatcag cgccgacaga ctcaccagaa agcttctgcg aaacatcgtg gacactccgg
     1681 ccggatcact ccagcgtcac ccgtgcgaac ttgcgcttgc cgacttgtac gacataggtt
     1741 ccggcgccga gtttcaaacc ccggtcgccg acgaccgcac cgtcgacccg cacgcccccg
     1801 ccgtcgacca ggcgcaggcc ttcgccggtc gacgccgcga gattagcctg tttcagcagc
     1861 gcgccgatgc ccagcggcgc cccggcgagc gtgaccgcgt cgatcacgtc cggcacgccg
     1921 ccgcgggcgc ggtgctcgaa gtcgagttcg gccgcgtcgg cagccgcggc accatgaaag
     1981 cgggtcgtga tctccttcgc gagcagcacc ttcacgtcct tcgggttccg gcccgccgcg
     2041 atctcggcct tgaagcgcgc gatctcggcc tcgggccgga agctcagcag cgtgaagtac
     2101 ttccacatca gcacgtcgct gatcgagagc agcttggcga acatgtcgtt cgcaggctcg
     2161 gtgatcgcga tgtagttgcc cttgctcttg gacatcttct cgacgccgtc gaggccttcg
     2221 agcagcggca tcgtcaggat gcactgcggc tcctggccgt actcggcctg cagcgcgcgg
     2281 ccgaccagca ggttgaactt ctggtcggtg ccgccgagtt cgatgtcgga ccgaagcgcg
     2341 accgagtcgt agccctgcat cagcgggtac aggaactcgt gcacgctgat cggcgtgccg
     2401 gccttgaagc gggcgtggaa gtcgttgcgc tccatcatgc gggcgaccgt gtagcgcgag
     2461 gcaagctgga tcatgccgcg cgcgccgagc gggtcgctcc actcggagtt gtagcggact
     2521 tcagcgcgct cgatgtcgag caccaagctg atctggtcga agtaggtgtt cgcgttcgcc
     2581 tcgatctgct cgcgggtcag cggcgggcgg gtcgtgttgc ggcccgaggg gtcgccgatc
     2641 atcgaggtga agtcgccaat cagcgggatc accgtgtggc cgaggtcctg caactgacgc
     2701 agcttgttga aaaccacagt gtggccgagg tgcaggtcgg gcgcagtcgg gtccagaccg
     2761 aacttgattc tgagtggcac gccggttgct tccgaccgtg caagcttggc cagccactcg
     2821 gcttgcggca gcagttcgtc gcagccgcgc agcgagatcg ccatcgccgc ctcgacccga
     2881 tctgtcaccg ggtggacggg gccggatggg gcggctgctg aagcttgact ggaaggctgg
     2941 gacatacggg gatttgacgg ggatgggcgc agggccaggt cgttatactt cgcggttgtc
     3001 acggagcccg ctcaccggcg tggtggatgc gatccgggca cgacatccgc gtcgccccag
     3061 aggcaggcgc cacggattct agtagaggcc cctcggggcc gcagggacaa aaaccgaagt
     3121 gcgcggccac gctgtcgcca ctcgcacaga gcctcgagcg ctcctgaacc gaattgaaat
     3181 tgctggacct ccttgaccgc gccgtcgctt cggccaccgc gcaaacgggc cgcttcgttt
     3241 ccgcacatcc gcgcagcctg tcggctgcgg tgatgctggg ttgcgccggc ttcggcgtca
     3301 ccgccttcgg catcgcgccg atggcgcccg acgccgctga catcccgaaa cgtctcgtga
     3361 ccgaaaccgt cacgcccgac gggattcagt cgcaactcga cgcgctcgcg gaacacgaac
     3421 tcgagcttta cagaaccgac ctgacccgcg ccagcgacac ggtcgacagc ctgctgaagc
     3481 gcctgaacgt cagcgacgct ggcgccgcac agttcctgcg cagcgacacg accgcgcgca
     3541 agctgctcga aggccgcggc ggcaagatgg tccgcgtaca gaccgacgag aacggcgtcc
     3601 tgaccgaact ggtggcgcgc tatgcgccgg ccgaccccga gcagctcggc acgtacttca
     3661 cccgcctgac ggtcaagcgc atcgccggca agctgctggc gatggtcgag accgcgccgc
     3721 tggcctcgca ggtgcgcatg ggcagcggca cgatccgcag ctcgctgttc gcggcgaccg
     3781 acgaggcgcg catccccgac ggcgtcgcca cacagctcgc cgaggtgttc gcgaccgacc
     3841 tcgacttcca ccgccagctg caggccggcg acaccttctc ggtgctctac gaagccctca
     3901 cggccgacgg cgagccgatc acctggagcg gttcggccgc gggcaaggtg ctcgccgccg
     3961 acttcgtcaa caacggcaag acctactcgg cgatctggtt caagggcgcc gacggcaagg
     4021 gtggctacta cggcttcgac ggccacagca agaagcgcgc gttcctcgcc agcccgatgg
     4081 agttctcgcg cgtgacctcg ggcttcgcga tgcgcatgca cccgatcctg aacagctgga
     4141 agcagcacaa gggcgtcgac tacggcgcgc cgagcggcac cgcgatccgc agcgtcggcg
     4201 acggcacggt cgacttcgcc ggctggcaga acggctacgg caacgtcgtc gagatccgcc
     4261 acagcgcgca gcgttcgacg ctgtacgcgc acatgagccg catcgacgtg aaacgcggcg
     4321 cgcacatcga ccagggccag cgcatcggcg cggtcggcgc gaccggctgg gccaccgggc
     4381 cgcacctgca cttcgaggtc aaggtcgacg gggtccagca gaacccgctg atcatggcca
     4441 aggcctccga gacgatcccg ctcgcgtcgg cgcagaaggc cgagttcgcg cagctggcga
     4501 agcgtctgaa gggccagctc gaggtcgccg attcgcttgc acggtcgggc gcctacgccg
     4561 agtgaccgcc tcgcatcgcc ggttcgaggg cccgccgctg cgggccttct caatggcgcc
     4621 ggcacaatcg gcgcatgact gagcgctaca tcgggttgat gtcgggcacc tcgctcgacg
     4681 gggtcgacgg cgtgctggtg gatttcgacg ccgcgccgat gcgcgtcgtc gcgcacgcgc
     4741 atcgcgcgtt cgagcctgcg ctcgcgcacg agctgctggc gctgaacgcg cccgg
//
LOCUS       DASRDG010000518         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1590313, whole genome shotgun sequence.
ACCESSION   DASRDG010000518 DASRDG010000000
VERSION     DASRDG010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590313"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1174)
                     /locus_tag="VFA35_08720"
     CDS             complement(191..1174)
                     /locus_tag="VFA35_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZT56294.1"
                     /translation="MSELHKFLFEGLPVRGMLVRLTDGWREVLARRAASGEAFPAPVR
                     ALLGEMAAAGVLMQGNIQFAGALVLQMYGDGPVKLAVAEVRSDLAFRVTAKVVGAVAD
                     DARLEAMLNVHGQGRCAITLDPQDKRPGQQPYQGVVALHGDHREPLQQLGEVLEHYML
                     QSEQLDTRLILAADDQVAAGLLIQRLPVQGGANLQGARRNEDLIGLDEAYKRIALLAG
                     TLTRAELLTLDADTILRRLFWEEPIRRFAPLRGEDGEGGPRFVCSCSRERVGNMLKGL
                     GRAEIDGIVTEQGRVEIGCDFCGATYHFDPVDVGELFTPAERQAPGSDAKH"
     gene            complement(1171..1695)
                     /locus_tag="VFA35_08725"
     CDS             complement(1171..1695)
                     /locus_tag="VFA35_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZT56295.1"
                     /translation="MALYQLDEHRPQLAAGAWVADSAQLIGRVELAEDASVWFGAVLR
                     GDTELLKIGKGSNVQDGSVLHADLGYPLTLGENVTVGHQVMLHGCTVGDGSLIGIQAV
                     VLNGAKIGRHCLVGAGALVTEGQTFPEGSLIIGAPAKVVRQLTPEQIARLRLSAEHYV
                     ANARRFAAGLKKFA"
     gene            1735..2517
                     /locus_tag="VFA35_08730"
     CDS             1735..2517
                     /locus_tag="VFA35_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZT56296.1"
                     /translation="MTARLRALEILCLADPVAKAAQARALFAGLDDAAIDPAERLANP
                     GELPGRPALPRLLHAKEIPSRSPFTPEGRAAMLHAVTHIEFNAINLALDAVWRFAGMP
                     IAYYRDWLRVAAEEALHFTLLREHLHSLGVDYGAFDAHDGLWAMAQRTAHDVTARMAL
                     VPRTLEARGLDAAPPMQAKLRRCGDLRAVEILDVILRDEVGHVAIGNHWYRWLCARDG
                     LDPVALYAELVVRHAAPRLRPPFNRAARRAAGFTDAELELLT"
     gene            2514..>3625
                     /locus_tag="VFA35_08735"
     CDS             2514..>3625
                     /locus_tag="VFA35_08735"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HZT56297.1"
                     /translation="MTTIFIAGFHHETNTFAPSPADWAAFTCGAGYPAYARGDAMLAQ
                     MAPTAQAIGGFIAEALEHGWTLKPSVWAGAMPSNRVTADAFERICAEIVEDLRAGGFD
                     AVFLDLHGAGVAECADDAEGELLARVRAVVGPSMPIVTALDLHANVTERMLTLASAMT
                     AYRTYPHVDMRATGARAARMLARRLVPGALGRTLHAERVPFLLPLNAQCTLVQPAAAA
                     IALIEQIEAQHDVELNFAMGFSAADFAECGPVLFGYGVDPLRVRTAVQQLHAEVAVTR
                     RADWKLEWLAPRDAVEQAIALALEAGDDAGPVVIADTQDNPGAGGSGDTTGLLAALLT
                     FRAPRSVLGLLYDPEAAVRAHQAGEGRELEFSLGGRS"
BASE COUNT          479 a         1363 c         1273 g          510 t
ORIGIN      
        1 gccgccgacc tcggccagaa ggcgaaagac gccgccgagc gcgcccgcga gcaggcgcgc
       61 cagacggcgg ccgccgcgca ggcgctcacc tgcccccaat gcggggctgc ggtgggccgg
      121 gacgacctgt tctgcgccgt ctgcggccac aagctgaaat agcgcggcgc cggcgtgcgc
      181 gcggcggagc tcagtgcttc gcgtccgagc ccggcgcctg gcgctcggcc ggcgtgaaca
      241 gctcgccgac gtcgaccggg tcgaagtggt aggtcgcgcc gcagaagtcg cagccgatct
      301 cgacccggcc ctgctcggtg acgatgccgt cgatctcggc gcgccccagg cccttcagca
      361 tgttgccgac gcgttcacgc gaacagctgc agacgaagcg cgggccacct tccccatctt
      421 cgccgcgcag cggcgcgaag cgccggatcg gctcctccca gaacaggcgg cgcaggatcg
      481 tgtcggcgtc gagtgtcagc agttccgcgc gcgtcagcgt gccggcgagc agcgcgatgc
      541 gcttgtaggc ctcgtccagg ccgatcaggt cttcgttgcg ccgcgcgccc tgcaggttgg
      601 ccccgccctg caccggcagg cgctggatca gcaagccggc ggcaacttgg tcgtcggccg
      661 cgaggatgag ccgggtgtcg agctgctccg actgcagcat gtagtgctcc agcacctcgc
      721 cgagctgctg cagcggctcg cggtggtcgc cgtgcagcgc gacgacgccc tggtacggct
      781 gctggccggg ccgcttgtcc tgcgggtcga gcgtgatcgc gcagcggccc tggccgtgca
      841 cgttgagcat cgcctcgagc cgcgcgtcgt cggcgaccgc gccgacgacc ttcgcggtga
      901 cgcgaaacgc caggtcgctg cgcacttcgg cgaccgcgag cttgaccggg ccgtcgccgt
      961 acatctgcag caccagcgcc ccggcgaact ggatgttgcc ctgcatcagc acgccggccg
     1021 cggccatctc gcccagcagc gcgcgcaccg gcgccgggaa cgcctcgccc gacgcggcgc
     1081 ggcgcgccag cacctcccgc cagccgtcgg tcaggcgcac gagcatgccg cgcaccggca
     1141 acccttcgaa cagaaatttg tggagttcgc tcatgcgaat ttcttcaagc cggcggcgaa
     1201 ccgccgcgcg ttcgccacgt agtgctcggc gctgagccga aggcgggcga tctgctcggg
     1261 cgtgagctgg cgcacgacct tggcgggagc gccgatgatc agcgagccct cggggaacgt
     1321 ctggccctcg gtgaccagcg cgccggcgcc gaccaggcaa tgcctgccga tcttcgcgcc
     1381 gttcagcacg acggcctgga tgccgatcag cgagccgtcg ccgaccgtgc agccgtgcag
     1441 catcacctgg tggccgaccg tgacgttctc gccgagcgtg agcgggtagc ccaggtcggc
     1501 gtgcagcacg ctgccgtcct gcacgttcga gcccttgccg atcttcaaca actcggtgtc
     1561 gccgcgcagg acggcgccga accacacgct cgcgtcctcg gcaagctcga cgcggccgat
     1621 cagctgcgcg ctgtcggcaa cccaggcgcc cgcggcgagc tgcggtcggt gttcgtcgag
     1681 ctggtacagc gccatgcagg tttgccggtg gtggtgaccg ataattctag ggtgatgacg
     1741 gcaaggcttc gcgcgctcga gatcctgtgc ctcgccgacc cggtcgcgaa ggcggcgcag
     1801 gcgcgcgcgc tgttcgccgg cctcgatgac gcggccatcg acccggccga acgcctggcc
     1861 aatccgggcg agctccccgg gcgccctgcg ctgccgcgcc tgttgcacgc gaaagagatc
     1921 ccctcgcgct cgcccttcac ccccgagggc cgcgccgcga tgctgcatgc cgtcacccac
     1981 atcgagttca acgcgatcaa cctcgcgctc gacgcggtct ggcgttttgc cggcatgccg
     2041 atcgcctact accgcgactg gctgcgcgtc gccgccgagg aggcgctgca cttcacgctg
     2101 ctgcgcgagc acctgcactc gctcggcgtc gactacggcg ccttcgacgc gcacgacggc
     2161 ctgtgggcga tggcgcagcg caccgcgcac gacgtcaccg cgcgcatggc gctggtgccg
     2221 cgcacgctcg aggcgcgcgg cctcgacgcc gcgccgccga tgcaggccaa gctgcgcagg
     2281 tgcggcgacc tgcgcgccgt cgagatcctc gatgtgatcc tgcgcgacga ggtcggccat
     2341 gtcgcgatcg gcaaccactg gtaccgctgg ctgtgcgcgc gcgacgggct cgacccggtg
     2401 gcgctgtacg ccgagctcgt cgtgcgccac gcggcgccgc gcctgcgccc gcccttcaac
     2461 cgtgccgcgc gccgcgccgc gggcttcacc gacgccgaat tggagctgct gacgtgacca
     2521 cgatcttcat cgccggcttc caccacgaga ccaacacctt cgcgccctcg ccggccgact
     2581 gggccgcgtt cacctgcggc gcgggctacc cggcctacgc ccgcggcgac gcgatgctcg
     2641 cgcagatggc gccgaccgcg caggcgatcg gcggcttcat cgccgaggcg ctcgagcacg
     2701 ggtggacgct gaagccctcg gtctgggccg gtgcgatgcc gtcgaaccgg gtcacggccg
     2761 atgccttcga gcgcatctgc gccgagatcg tcgaagacct gcgcgccggc ggcttcgatg
     2821 cggtctttct cgacctgcac ggcgccggcg tcgccgagtg cgccgacgat gccgagggcg
     2881 agctgctggc gcgcgtgcgc gcagtcgtcg gcccctcgat gcccatcgtc accgcgctcg
     2941 acctgcatgc caacgtgacc gagcgcatgc tcacgctcgc gagcgcgatg accgcgtacc
     3001 gcacctaccc gcacgtcgac atgcgtgcga ccggcgcgcg cgccgcgcgc atgctcgcgc
     3061 gccggctcgt gcccggcgcg ctcgggcgca cgctgcacgc cgagcgcgtg ccgttcctgc
     3121 tgccgctgaa cgcgcagtgc acgctggtgc agccggccgc cgcggcgatc gcgctgatcg
     3181 agcagatcga ggcgcagcac gatgtcgaac tcaacttcgc gatgggcttc tcggccgccg
     3241 actttgccga gtgcgggccg gtgctgttcg gctacggcgt cgaccccttg cgcgtgcgca
     3301 cggcggtgca gcagctgcac gccgaggtcg cggtgacgcg ccgcgccgac tggaagctcg
     3361 aatggctcgc gccgcgcgac gcggtcgaac aggcgatcgc gctggcgctc gaagccggcg
     3421 acgatgccgg gccggtcgtg atcgccgaca cccaggacaa ccccggcgcc ggcggcagcg
     3481 gcgacaccac cgggctgctc gccgcgctgc tcaccttccg cgcaccgcgc tcggtgctcg
     3541 gcctgctcta cgaccccgag gcggcggtgc gcgcgcacca ggcgggcgag gggcgcgagc
     3601 tcgaattctc gctcgggggg cgttc
//
LOCUS       DASRDG010000519         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_439111, whole genome shotgun sequence.
ACCESSION   DASRDG010000519 DASRDG010000000
VERSION     DASRDG010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439111"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFA35_08740"
     CDS             complement(<1..409)
                     /locus_tag="VFA35_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZT56298.1"
                     /translation="MRTLLPQPKPQRDPFLSLFDSFLLPQLFADDVEPRTDIAETDQA
                     YELSFELPGLDEKDIQVQLHDRQLTVTAERREEQKSEGKTWHRVERRYGKLSRTVLLP
                     EPARSDAVDASYKQGVLIVTVQKSPERLPTKVQV"
     gene            complement(597..>2008)
                     /locus_tag="VFA35_08745"
     CDS             complement(597..>2008)
                     /locus_tag="VFA35_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZT56299.1"
                     /translation="QSGQIPDATVSAVVDACRRFKASCDRLAVATVRAIATAAMREAR
                     NRDLVVDRVREATGIEVEVISGSQEAYLLAQAVQTRIDLQRGRSVLVDVGGGSVEVVL
                     VEDGQVTGADSYRLGALRMLATLPDPNATGESFVELLQQHLHGLERRIADRFDGRRID
                     RYVAVGGNIETIADLVGGRTDARGAAAPMSTVPLASVRAETMALAQLSVDERMTKKDL
                     KPDRADTIVPAGVVYARIGEIAGTDAVLVPRVGIKDGLLAELVAGHASAFTPEEHVDT
                     VLSACRALSRKFHGEGEHGETVLQLARQIFDQTAALHGLGGRARVLLEAAALLHDIGV
                     TVNNDGHHKHSQYLIEASEIVGLAEEERHLVALLARYHRKGPPSRDHEEFSALRRRER
                     SLVERLAAILRLADALDRQHAGIVRAVQVRLRERTVELMPTLAPGQRTRLTLERRAVE
                     EKGAMFGQLFDRSVELLPP"
BASE COUNT          274 a          691 c          718 g          325 t
ORIGIN      
        1 tgacctgcac cttcgtcggc aggcgctcgg ggctcttctg caccgtcacg atcaggacgc
       61 cctgcttgta gctggcatcg accgcgtccg aacgcgccgg ctccggcagc agcacggtgc
      121 gcgacagctt gccgtagcgg cgctcgacgc ggtgccaggt cttgccttca ctcttctgct
      181 cctcgcgccg ctcggccgtc acggtgagct ggcggtcgtg cagctgcacc tggatgtcct
      241 tctcgtcgag gccaggcagc tcgaacgaca gttcgtaggc ctggtcggtc tcggcgatgt
      301 cggtgcgcgg ctcgacgtcg tcggcaaaca gctggggcag gaggaacgag tcgaagaggc
      361 tgaggaacgg atcgcgctgg ggcttgggtt ggggcaacag ggttcgcatg tttcgggtcc
      421 ttgggtgaaa gatgctgccc ggtatgcaac gtcgatgcca acgccagacg aaccgtcaga
      481 ctaccatgct agtgtctgcg cagtgttgct ttgcgtcgct ctacagcgct caggatcgta
      541 gccgccatgg tgacgtgttg gcgcgccagt gccaatctgg ccgatcgcct cggatcctag
      601 ggcggcagca actcgacgct gcggtcgaac agctggccga acatggcgcc cttctcctcg
      661 acggcgcggc gttcgagcgt caggcgcgtt cgttggcctg gcgccagcgt cggcatcagc
      721 tcgaccgtgc gctcgcgcag gcgtacctgc acggcgcgca cgatgcctgc gtgctggcgg
      781 tccagcgcgt cggcgagccg caggatcgcc gcgagccgtt cgaccagcga gcgctcgcgc
      841 cggcgcagag ccgagaactc ctcgtggtcg cggctcggcg ggcccttgcg gtggtagcgc
      901 gcgagcagcg cgacgaggtg ccgctcctct tcggcgaggc cgacgatctc gctggcctcg
      961 atcaggtact gcgagtgttt gtggtggccg tcgttgttga cggtgacgcc gatgtcgtgc
     1021 agcagcgccg ctgcctcgag caggacgcgc gcacggccgc cgagcccgtg cagcgccgcc
     1081 gtctggtcga agatctggcg cgcgagctgc agcaccgtct cgccgtgctc cccctcgcca
     1141 tggaacttgc gcgacagcgc gcggcacgcg gacagcaccg tgtcgacgtg ctcctcgggc
     1201 gtgaaggcgc tggcgtgacc cgcgacgagc tcggccagca ggccgtcctt gatgccgacg
     1261 cgcggcacga gcacggcgtc ggtgcccgcg atctcgccga tgcgggcgta gaccacgccc
     1321 gccggaacga tcgtgtcggc gcgatcgggc ttgaggtcct tcttggtcat gcgctcgtcg
     1381 acgctcaact gcgccaacgc catcgtctcg gcgcgcacgc tcgcgagcgg caccgtcgac
     1441 atcggcgcgg cggccccgcg cgcgtcggtg cgcccgccca cgaggtcggc gatggtctcg
     1501 atgttgccgc cgacggcgac gtagcggtcg atgcggcggc cgtcgaaacg gtcggcgatg
     1561 cgccgttcga gcccgtgcag gtgctgctgc aacagttcga cgaacgactc gccggtcgcg
     1621 ttcggatcgg gcagcgtcgc cagcatgcgc agcgcaccga ggcgatagga gtcggcgccc
     1681 gtcacctggc cgtcctcgac gagcacgacc tcgacgctgc cgccgccgac gtcgacgagc
     1741 accgaacggc cgcgctgcag atcgatgcgc gtctgcaccg cctgcgcgag caggtacgcc
     1801 tcctggctgc cggagatgac ttcgacctcg atgcccgtgg cctcgcggac gcggtcgacg
     1861 acgaggtcgc ggttgcgggc ctcgcgcatc gccgcggtcg cgatcgcgcg cacggtggcc
     1921 accgcgagcc tgtcgcacga ggccttgaag cgccggcacg cgtcgacgac ggcggacacg
     1981 gtggcgtccg ggatctgccc cgactgga
//
LOCUS       DASRDG010000520         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1590397, whole genome shotgun sequence.
ACCESSION   DASRDG010000520 DASRDG010000000
VERSION     DASRDG010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590397"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFA35_08750"
     CDS             complement(<1..603)
                     /locus_tag="VFA35_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZT56300.1"
                     /translation="MMTLAQAHALLPDSTLVGDSATELLRVHSDTRSLQRGDLFVALR
                     GERFDAHDFLAQARASGAAAALAERGLAEAGLSGLQVADTQAALTSLAHAWRRRFHLP
                     LIAVTGSNGKTTVTQMIATILRAWLGDAAFATAGNLNNHIGVPLTLLRLRQDDDVHHR
                     AGVVELGMNHAGEIKALAELAAPTVALVNNAQREHQEFMAS"
     gene            complement(600..2141)
                     /locus_tag="VFA35_08755"
     CDS             complement(600..2141)
                     /locus_tag="VFA35_08755"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZT56301.1"
                     /translation="MALRELASVSDALGWFAERGVRALAIDSRRVAPGDAFIAWPGYA
                     VDGRQFVPQALAAGASACLVEADGADAFDGLDRAEVAAMRKLKAATGPLASAFMGEPS
                     AKLEVVAVTGTNGKTSTAWWVAQALSALGRRCGVIGTLGIGEPPHGRAAGGEPPSPLQ
                     PAAITFTGMTTPGPVTVQAGFKRFADAGFKACAIEATSIGISEHRLAGTEIDVALFTN
                     FTQDHLDYHGSMGAYWAAKARLFAWPGLRAAVVNVDDPQGVQLARSLQDTALDLWTYS
                     TRGEAQLRASRIGYDAGGLAFELREGASTVAVKTQMIGEYNVANLLATIGGLRACGVP
                     LADAAAACKGLTPVPGRMQRVASDAAAPQVVVDYAHTPDALEKALLALQPFATERGGA
                     LWCVFGCGGDRDQGKRPLMGALAQRLATQVVVTSDNPRHEAPAAIVAQIVAGLVGSQE
                     RVAVIEDRRAAIAHALRHADARDVILVAGKGHEDYQEINGVKQHFSDVEEAAVALRLR
                     EGARA"
     gene            complement(2141..>2636)
                     /locus_tag="VFA35_08760"
     CDS             complement(2141..>2636)
                     /locus_tag="VFA35_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZT56302.1"
                     /translation="QIAHAYTVFAHDGEMIPVTLSKSAAVGEEARVAGLRVISPQTAK
                     TVREMLALVTQPGGTAPKAQTIGYSVGGKSGTAYKQEGKGYATKKYRAWFVGIAPIGN
                     PRIIVAVMVDEPSAGKWYGGDVAAPVFSDLVQQTLRTLNVQPDLDVKTQIVAKDVPAE
                     EESF"
BASE COUNT          351 a          945 c          935 g          405 t
ORIGIN      
        1 actggccatg aactcctggt gctcgcgctg cgcgttgttg acgagcgcga cggtcggcgc
       61 ggccagttcg gccaacgctt tgatctcgcc cgcgtggttc atgccgagtt cgacgacgcc
      121 ggcgcggtgg tgcacgtcat cgtcctgccg cagccgcagc aaggtcagcg gcacgccgat
      181 gtgattgttc agattgccgg cggtcgcgaa ggccgcgtcg ccgagccagg cgcgcaggat
      241 cgtcgcgatc atctgcgtca ccgtcgtctt gccgttgctg ccggtcaccg cgatcagcgg
      301 caggtggaag cggcggcgcc aggcgtgcgc gagggaagtc agcgcggctt gcgtgtcggc
      361 cacttgcagg cccgacaggc cagcctcggc gagcccacgt tcggccagcg ccgcagcagc
      421 gccgctggcc ctggcttgcg caaggaagtc gtgcgcgtcg aagcgctcgc cgcgcaacgc
      481 gacgaacagg tcgccgcgtt gcaggctgcg ggtgtcgctg tgcacgcgca gcagttcggt
      541 cgcgctgtcg ccgacgagcg tcgaatcggg cagcagcgcg tgcgcttgcg cgagcgtcat
      601 catgcgcgcg ccccttcgcg caggcgcagc gcgacggccg cttcctcgac gtccgaaaag
      661 tgctgcttca cgccgttgat ctcttggtag tcctcgtggc ctttgccagc gaccaggatc
      721 acgtcgcgcg catcggcgtg gcgcaacgcg tgggcgatcg cggcgcggcg gtcttcgatc
      781 acggccacgc gctcttgcga gccgacgagc ccggcgacga tctgcgcaac gatcgccgcc
      841 ggcgcctcgt ggcgcgggtt gtcgctggtg accacgacct gggttgcgag gcgctgcgcg
      901 agcgcgccca tcagcgggcg cttgccttga tcgcggtcgc cgccgcagcc gaacacgcac
      961 cacagcgcgc cgccgcgctc ggtggcgaag ggttgcagcg caagcagcgc cttctcgagc
     1021 gcgtcgggcg tgtgcgcgta gtcgacgacg acctgcggtg cggcggcgtc cgacgcgaca
     1081 cgctgcatgc ggccgggcac cggcgtcaac cccttgcagg ccgcggcggc atcggcgagc
     1141 ggcacgccgc aggcgcgcag gccgccgatc gtcgcgagca ggttcgcgac gttgtattcg
     1201 ccgatcatct gcgtcttcac cgcgaccgtg ctcgcgcctt cgcgcagctc gaaggccaga
     1261 ccgcccgcgt cgtagccgat gcgcgacgcg cgcagttggg cctcgccgcg cgtcgagtag
     1321 gtccacaggt cgagggcagt gtcctgcagc gaacgcgcga gctgcacgcc ctgcgggtcg
     1381 tcgacattga cgaccgcggc gcgcagcccg ggccacgcga acaggcgcgc cttcgcggcc
     1441 cagtaggcgc ccatcgagcc gtggtagtcg aggtggtcct gggtgaagtt ggtgaacaac
     1501 gcgacatcga tctcggtgcc ggcgaggcgg tgttcgctga tgccgatcga ggtggcctcg
     1561 atcgcgcagg ccttgaatcc ggcgtcggcg aagcgcttga agccggcctg caccgtgacc
     1621 gggccgggcg tggtcatgcc ggtgaaggtg atcgcggccg gctgcaacgg cgatggtggc
     1681 tcgccccccg ccgcacggcc gtgcggcggc tcaccgatac cgagcgtccc gatcacgccg
     1741 cagcgccggc ccagcgcgct caacgcctgc gccacccacc acgcggtcga ggtcttgccg
     1801 ttggtgccgg tgacggcgac gacctcgagc ttggcgctcg gctcgcccat gaaggcgctc
     1861 gcgagcgggc cggtcgcggc cttcagcttg cgcatcgccg cgacttccgc gcggtcgagc
     1921 ccgtcgaatg catccgcgcc gtcggcttcg accaggcagg cgctcgcgcc ggccgccagc
     1981 gcctgcggca caaactgccg gccgtcgacc gcgtagcccg gccacgcgat gaaggcgtcg
     2041 cccggcgcga cccggcggct gtcgatcgcg agcgcgcgca cgccgcgttc ggcgaaccag
     2101 ccgagcgcat cactcaccga agccaactcg cgcaaggcca tcagaaactc tcctcctcgg
     2161 ccggcacgtc cttcgcgacg atctgcgttt tgacgtcgag gtcgggctgc acgttcagcg
     2221 tgcgcagcgt ctgctgcacg agatcgctga acaccggcgc ggcgacgtcg ccgccgtacc
     2281 atttgcccgc gctgggctcg tcgaccatca cggcgacgat gatgcgcggg ttgccgatcg
     2341 gcgcgatgcc gacgaaccag gcacggtact tcttggtcgc gtagcccttg ccctcctgct
     2401 tgtacgcggt gcctgacttg ccgccgaccg agtagccgat ggtctgcgcc ttcggcgccg
     2461 tgccgcccgg ctgggtcacg agggccagca tctcgcgcac ggtcttcgcg gtctgcggcg
     2521 aaatcacgcg caggccggcc acgcgtgcct cctcgccgac cgccgcgctc ttgctcagcg
     2581 tgaccgggat catctcgccg tcgtgcgcga acacggtgta cgcgtgcgcg atctgg
//
LOCUS       DASRDG010000521         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1004297, whole genome shotgun sequence.
ACCESSION   DASRDG010000521 DASRDG010000000
VERSION     DASRDG010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004297"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFA35_08765"
     CDS             complement(<1..687)
                     /locus_tag="VFA35_08765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZT56303.1"
                     /translation="MNRLDLVMIVRDEARCLVRCLASARPWVDGIVVLDTGSVDATVE
                     IARRYGARVEQFEWIDDFAAARNAALAASDAPWRLVLDADEWIAEGGESLAEVRREEP
                     AFVGQIRVASLFDAAGGGDVAAAPSWLPRLLPRGVVYRGRIHEQPESALPRRRLPLEI
                     GHDGYLDAQKATKAGRNERLLRLALAAEPKDAYLHYQLGKDLELASRFAEAEPHYSMA
                     LARCELRAGWR"
     gene            1082..1885
                     /locus_tag="VFA35_08770"
     CDS             1082..1885
                     /locus_tag="VFA35_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZT56304.1"
                     /translation="MTMSINSNIVSLNAQRHLTESQSSLATSMERLSSGLRVNSSKDD
                     AAGLAIADRMNAQIRGTNVAIRNANDGISLAQTAEGALATVTDALQRMRELAVQAQNG
                     SNSSGDRANLDTEYQQLSAEITRIAAQTKFNGVAIVGASAGAQVFQVGANNGDTLTIT
                     TSTVTTVAGGLTTAALASTAVAALDTALDTVTTQRATYGAAISRFGMAISNLSITGEN
                     QSAARGRIMDADFATETANLSRAQILQQAGSAMVAQANQLPNSVLTLLR"
     gene            2039..>2576
                     /locus_tag="VFA35_08775"
     CDS             2039..>2576
                     /locus_tag="VFA35_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HZT56305.1"
                     /translation="MPQTINTNITSLNSQRHLNTTQSSLSTSMERLSSGLRVNSAKDD
                     AAGLAIADRMNAQIRGTNVAIRNANDGISLAQVAEGALATVTDALQRMRELAVQAQNG
                     SNGTGDRSNLDTEYQQLSAEITRIATQTKFNGNAIVGAGAGAQTFQVGANNGDTLTVT
                     TTQVTTVGGDLTTAANAST"
BASE COUNT          467 a          905 c          834 g          370 t
ORIGIN      
        1 gcgccagccg gcgcgcagct cgcagcgggc gagcgccatt gaatagtgcg gctcggcctc
       61 ggcgaagcgc gaggccagct cgaggtcctt gccgagctga tagtgcagat acgcgtcttt
      121 gggctcggcc gcgagcgcca ggcggagcag gcgctcattg cggccggcct tggtcgcctt
      181 ctgcgcatcg agatagccgt cgtggccgat ctcgagcgga aggcgccggc gaggcagcgc
      241 gctctcgggc tgctcgtgga tgcggcctct atagacgacg ccgcgcggca gcagccgcgg
      301 cagccagctc ggcgccgcgg cgacgtcgcc gccaccggcc gcgtcgaaca ggctggcgac
      361 gcggatctgg ccgacgaatg cgggctcctc ccgtcgcacc tcggcgagcg attcgccgcc
      421 ttccgcgatc cattcgtcgg cgtcgagcac caggcgccag ggcgcatcgc ttgcggccag
      481 cgcggcattg cgggcggcgg caaagtcgtc gatccactcg aattgctcga cccgtgcacc
      541 gtagcggcgc gcgatctcga ccgtggcgtc gaccgagccg gtatcgagca cgacgatgcc
      601 gtcgacccac ggccgcgcgc tcgccaggca gcgcacgagg cagcgcgcct cgtcgcgcac
      661 gatcatcacc aagtcgagcc gattcatggc gtgatcgaaa catgccgata tcgccgccga
      721 tgcagcgaac agtccccgtc gaaccgcctt tctctaccgt ttccagaggt ttcttggacg
      781 cgctgggctg cggcgcattg cacaccctct tcggtttgcc ctcaagttcg ccggactggg
      841 ctcgataacc ctcccaacgc acctggcaga tcgggttcgt ggtccggtta gccggctgaa
      901 aggttgggca gcaaagggag ctgccggaca caacggttga ctagcgcggg cggtgtcgaa
      961 gcaggaggcg cgagccgaat gcaacgacat gcaacggtgg tcgtggaggt tcgctttcgc
     1021 ggcgaccgcg gtaacgccgg cggctaggcc gggagtcaat taacctaagg agtctttttt
     1081 catgacgatg agtatcaaca gcaacatcgt ttcgctgaac gcgcaacgac acctgacgga
     1141 gtcgcaaagc tcgctcgcga cctcgatgga gcgcctgtcg tccggccttc gtgtcaacag
     1201 ctcgaaggac gacgccgccg gcctggcgat cgccgaccgc atgaacgcgc agatccgcgg
     1261 caccaacgtc gccatccgca atgccaacga cggcatttcg ctggcgcaga ccgctgaagg
     1321 cgcgctcgcc accgtgaccg atgcgctgca gcgtatgcgt gaactggcgg tccaggccca
     1381 gaacggctcg aacagctccg gcgaccgtgc caacctggac acggaatacc agcagctctc
     1441 ggccgaaatc acccgcatcg ccgcgcagac caagttcaac ggcgtcgcga tcgtcggcgc
     1501 cagcgccggc gcgcaagtgt tccaggtcgg cgccaacaac ggcgacacgc tgacgatcac
     1561 cacgtcgacg gtcaccacgg tggcaggcgg cctgacgacc gccgccctgg cctccacggc
     1621 ggtggctgcg ctcgacacag cgctcgacac ggtcaccacg cagcgtgcga cctacggcgc
     1681 tgcgatcagc cgcttcggta tggcgatctc gaacctgtcg atcaccggcg agaaccagtc
     1741 ggccgcccgc ggccggatca tggacgccga cttcgccacg gaaacggcca acctgtcgcg
     1801 ggcgcagatc ctgcagcagg ccggcagcgc gatggtggcg caagccaacc agctgccgaa
     1861 ctcggtgctc acgctcctgc gctgatcatc cgggaccgca accggtcttc gagccgccct
     1921 tcggggcggc tttcttcatt tccggctgcg aattggccgc aagaaaggct tcacttcccg
     1981 cgatcggtgc cgagggcccg ggaaaagaat ggcgccaacc ttccctccgg agtgcgccat
     2041 gccccagacg atcaacacga acatcacgtc gctgaactcg cagcggcacc tgaacacgac
     2101 ccagtcgtcg ctgtcgacct cgatggaacg gctctcctcg ggtcttcgcg tcaactcggc
     2161 caaggacgac gccgccggcc tggcgatcgc cgaccggatg aacgcgcaga tccgcggcac
     2221 caacgtcgcg atccgcaacg ccaacgacgg catctcgctg gcgcaggtcg ccgaaggcgc
     2281 gctcgccacc gtgaccgacg cgctgcagcg catgcgcgag ctggcggtgc aggcgcagaa
     2341 cggcagcaac ggcaccggcg accgttcgaa cctcgacacc gagtatcagc agctctcggc
     2401 cgaaatcacc cggatcgcga cccagacgaa gttcaacggc aacgcgatcg tcggtgccgg
     2461 agccggtgcg cagacgttcc aggtcggcgc caacaacggc gacacgctga ccgtgacgac
     2521 gacacaggtc acgaccgtcg gcggcgactt gaccacggcc gccaacgcgt cgacgg
//
LOCUS       DASRDG010000522         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1004365, whole genome shotgun sequence.
ACCESSION   DASRDG010000522 DASRDG010000000
VERSION     DASRDG010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004365"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFA35_08780"
     CDS             complement(<1..671)
                     /locus_tag="VFA35_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56306.1"
                     /translation="MRLPDLITSLIFTVLTLGCGNASADGPPLRLDHADFVASDAAEP
                     PPDSAAWQPQSLPDDWDAAHAGLRGIGWYRLRFELAPPLPARPAIYLPFLRNVGAVYL
                     NGALVGATGAFGRINPVAAPVLLAIEPQQLRAGGNTLHVRLWAPRGWLQAIDPVSIGE
                     RLVFETEMERERLVQVTLSQMAGAFSATVSLYMLVLWLRRRHDVIYGYFAAVALTHAL
                     YLLTTV"
     gene            complement(700..1929)
                     /locus_tag="VFA35_08785"
     CDS             complement(700..1929)
                     /locus_tag="VFA35_08785"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HZT56307.1"
                     /translation="MTPTARALALITVATFALGAHAPARADAVTDWNARAAGFSVEGR
                     IHPPHLATSMMASVQSAVFAATNAITKKYPASAMLDAAPGASVDAAVAAASRGVLVKY
                     APAAKEAIDRTYDAALASIADGAAKTAGIDVGERAAAAVLALRAGDLSLPADQYRPVT
                     TAGTYVPTVTPLATVWPQRKPWLMSSAAQFRPGPPPDLSSTIWARDYNEIKAVGARNS
                     TTRTAEQTAIAKFWEATGNPIYHSIVRSLADRPGRDITQNARLFAVVAQATDDALIAV
                     MDAKYHYMFWRPLTAIRNGDIDGNDATERDPSWTPLIDTPMHPEYPCAHCILAGSVGA
                     VLKAEIGSGVPGTLTSTNYLPGIAPRSWTSVDAMVDEVKNARIYDGVHYRNSTEVGAA
                     MGRQIGELAAAKFSLGK"
     gene            complement(2064..>2600)
                     /locus_tag="VFA35_08790"
     CDS             complement(2064..>2600)
                     /locus_tag="VFA35_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZT56308.1"
                     /translation="ARQLLATQAPDVLLTDLGLPDGSGIDLIRETARAHPATDIMVIS
                     VFGDEEHVVASIEAGATGYVLKDSLPEEFVALIRELRAGGSPISPLIARQLLRRWRPA
                     AAPAAAAATTPGDAGLSAREAEVLGLIAKGFNFAEIARLLTVSPHTITAHVKKIYQKL
                     AVHSRGEAVYEATKMGLL"
BASE COUNT          390 a          871 c          922 g          417 t
ORIGIN      
        1 acggtggtga ggaggtagag cgcgtgcgtc agcgccaccg ctgcgaagta gccgtagatc
       61 acgtcgtggc gccggcgcag ccacagcacc agcatgtaca ggctgaccgt ggccgagaac
      121 gcgccggcca tctgcgacag cgtgacctgc acgaggcgtt cgcgctccat ttcggtttcg
      181 aacaccagcc gttcgccgat gctcaccggg tcgatcgcct gcaaccagcc acgcggagcc
      241 cacagccgga cgtgcagcgt gttgccgcca gcgcgcagtt gctgcggctc gatcgcgagc
      301 agcaccggcg cggcgacggg attgatgcgg ccgaacgcgc ccgtcgcgcc gaccagcgcg
      361 ccgttcaggt agacggcacc gacgttgcgc agaaacggca ggtagatcgc cggcctggct
      421 ggcaaggggg gcgccagttc gaaacgcagg cggtaccagc cgatgccccg caggccggcg
      481 tgcgcggcgt cccagtcgtc gggcagcgat tgcggctgcc acgcggccga gtccggtggc
      541 ggctcggcgg cgtcgctggc cacgaagtcg gcgtggtcga ggcgcagcgg cgggccgtct
      601 gcggacgcgt tgccacagcc caacgtcagg accgtgaaga tcagggaagt gatcagatct
      661 ggcagccgca tcgtcgagcc ttcggtcggt ccgatcaggt cacttgccga gcgagaactt
      721 cgctgccgca agctcgccga tctgacggcc cattgccgca cccacctcgg tcgagttccg
      781 gtaatggacg ccgtcgtaga tgcgcgcgtt cttcacttcg tcgaccatcg cgtcgacgct
      841 ggtccagctt cgtggcgcga tgcccggcaa gtagttggtg gacgtgagcg tgcctggcac
      901 gccgctgccg atctcggcct tgagcactgc gccgaccgat cccgccagga tgcaatgcgc
      961 gcacggatac tcgggatgca tcggcgtatc gatcagcggc gtccacgacg gatcgcgctc
     1021 ggtggcgtcg ttgccgtcga tgtcgccgtt gcggatcgcg gtgagtggtc gccagaacat
     1081 gtagtggtac ttggcgtcca tcacagcgat gagggcatcg tcagtagcct gcgcgacgac
     1141 cgcgaacagg cgtgcgttct gcgtgatatc gcgccccggc cgatcggcca acgaacgcac
     1201 gatgctgtga tagatggggt tgcccgtggc ctcccagaac ttggcgatcg ccgtctgctc
     1261 ggcggtgcgc gtggtgctgt tgcgcgcgcc caccgccttg atctcgttgt agtcgcgtgc
     1321 ccagatcgtg ctcgacagat cgggcggtgg tccgggacga aactgcgccg cgctcgacat
     1381 caaccacggc ttgcgctgcg gccagaccgt tgccagaggc gtcaccgtgg ggacgtaggt
     1441 gcctgcggtc gtgaccggcc ggtattgatc cgcgggcaag ctcaagtcgc ccgcgcgcag
     1501 cgcaagcacc gcggctgccg cgcgctcgcc gacgtcgatg cctgcggtct tcgcggcgcc
     1561 gtcggcaatc gacgccagcg cggcatcgta ggtgcggtcg atcgcttcct tcgctgccgg
     1621 ggcgtacttg accagcacgc cgcggctggc ggccgcgacc gcggcgtcca ccgacgcgcc
     1681 cggcgcggca tcgagcatcg cgcttgcggg gtattttttc gtgatcgcgt tagtcgcggc
     1741 gaacacggcg ctctgcaccg acgccatcat ggaggtcgcg aggtgcggcg gatggatgcg
     1801 accttcgacc gagaagccgg ccgcgcgcgc gttccaatcg gtgacggcgt cggcacgcgc
     1861 cggcgcgtgc gcgccaaggg cgaaagtggc gacggtgatc agggcaaggg ctcgggcggt
     1921 cggtgtcatg ggtttctcca atgggtttca tcccggtcgg acggcgcaat cggcgcgtgc
     1981 ccagattcgt gatgcgccga tcgtaggcag gcttgcccgg ccgcgcatga cgtgaacacg
     2041 tgatgccgcc cgcaacgcga ccgttacagc aaccccatct tggtcgcctc gtacaccgcc
     2101 tcgccgcgcg agtgcaccgc gagcttctgg tagatcttct tcacatgcgc cgtgatcgtg
     2161 tgcggcgaca ccgtgagcag gcgcgcgatc tcggcgaagt tgaagccctt ggcgatcagg
     2221 cccagcactt cggcctcgcg cgccgagagc ccggcatcac cgggcgtcgt cgccgctgcc
     2281 gccgccggcg cggccgccgg ccgccagcgg cgcagcagct ggcgtgcgat cagcgggctg
     2341 atcggcgagc caccggcgcg cagttcgcgg atcagcgcga cgaactcttc gggcaggctg
     2401 tccttgagca cgtagccagt ggcgccggcc tcgatgctgg ccaccacatg ctcctcgtcg
     2461 ccgaagaccg agatcaccat gatgtcggtc gccggatggg cgcgagcggt ctcgcggatc
     2521 aggtcgatgc cgctgccatc gggcaggccc aggtcggtca gcagcacgtc aggcgcctgg
     2581 gtagcgagca gctggcgcgc
//
LOCUS       DASRDG010000523         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_310066, whole genome shotgun sequence.
ACCESSION   DASRDG010000523 DASRDG010000000
VERSION     DASRDG010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310066"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /gene="phnA"
                     /locus_tag="VFA35_08795"
     CDS             complement(<1..978)
                     /gene="phnA"
                     /locus_tag="VFA35_08795"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HZT56309.1"
                     /translation="MMINVNQRNYAAPKQPTVVVCVDGCEPDYLAQAVAHGKMPWLKR
                     VLAEGTVLVADCVIPSFTNPNNLSIVTGAPPSVHGICGNYLFDVATGTEVMMNDPKWL
                     RAPTLLAALADAGQSVAVVTAKDKLRKLLGHGMQGICFSAEKADEVTLVENGIADVLG
                     LVGMPVPSVYSAALSEFVFAAGVKLMQNEQKPDVMYLSTTDYVQHKHAPGTEGADAFY
                     SMMDRYLGQLDALGCVIALTADHGMNAKTRMDAQPDVIYLQDVLDDWLGAAKTRVILP
                     ITDPYVVHHGALGSFATVYLPGSVDQAALVRRLAAQRGIEAVLTRAEAAA"
     gene            complement(975..1715)
                     /locus_tag="VFA35_08800"
     CDS             complement(975..1715)
                     /locus_tag="VFA35_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate utilization associated
                     transcriptional regulator"
                     /protein_id="HZT56310.1"
                     /translation="MTATVVPHPTITLLQTSSLTSVVQGELERMILSGELAPGAKLTE
                     MALAARLGVSRGPLREAFRMLEEAGLVRTEKNRGVFVRDIPIEEAVEIFDLRAAMDEL
                     VGRRLAEHITPVQLKEIRGLVEQMEQAVKAKDADHYHLLNLKFHDRLVELAGNGKLTA
                     IYRKLIKELSLFRRLNLADGWLMPISANEHRQIVKAIASGDPDAAGKAMFDHVIESKE
                     RTVRNHQHPAHPTEPAPPKARALERTSP"
     gene            1885..>2634
                     /locus_tag="VFA35_08805"
     CDS             1885..>2634
                     /locus_tag="VFA35_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZT56311.1"
                     /translation="MNLGSIKPVLAALAFVTATGAFAQKTQLTVYTALETDQLKAYEA
                     GFTKVNPDIEIKWVRDSTGVITAKLLAEKANPQADAVMGVAASSLALLDKQGMLIPYA
                     PLNLDGIMSQYRDKKNPPAWFGMDVWGATVCFNTVEAQKKGLPKPETWKDLTKPIYKG
                     QIVMPNPASSGTGFFDVSAWLTLWGDNDGKGGGWKYMDALHENIAQYTHSGSKPCNMA
                     ATGEYVMGISFEYRGNTNKAKGAPIDLIFPKE"
BASE COUNT          451 a          860 c          860 g          463 t
ORIGIN      
        1 cgcggcggct tcggcgcggg tcagcacggc ctcgatgccg cgctgcgcgg cgagccgccg
       61 cacgagcgcg gcctgatcga cgctgcccgg caggtacacg gtcgcgaacg aaccgagtgc
      121 gccgtgatgc accacgtagg ggtccgtgat cggcaggatc actctggtct tggctgcacc
      181 gagccaatcg tccagcacgt cctgcaggta gatcacatcg ggctgcgcat ccatgcgcgt
      241 cttcgcgttc atgccgtggt cggcggtgag cgcgatcacg cagccgagcg cgtcgagctg
      301 accgaggtag cggtccatca tcgaatagaa cgcgtcggca ccttccgtgc ccggcgcgtg
      361 cttgtgctgc acgtagtcgg tcgtcgacag gtacatcacg tcgggtttct gttcgttctg
      421 catcagcttg acgccggccg cgaacacgaa ctccgacagc gccgcgctgt agaccgacgg
      481 caccggcatg ccgaccagcc cgagcacatc ggcgatgccg ttctcgacca gcgtgacctc
      541 gtcagccttc tcggcggaga agcagatgcc ttgcatgccg tggccgagca gcttgcgcag
      601 cttgtccttg gccgtgacga ccgcgacgct ttggccggca tcggccaatg cggccagcag
      661 cgtcggcgcg cgcaaccact tcgggtcgtt catcatgacc tcggtgcccg tggcgacgtc
      721 gaacaggtag ttgccgcaga tgccgtgcac cgagggcggc gcgccggtca cgatcgacag
      781 gttgttcggg ttcgtgaagc tcgggatcac gcagtcggcg accagcaccg tgccttcggc
      841 gagcacgcgt ttcagccacg gcatcttgcc gtgcgcgacg gcctgggcga ggtagtcggg
      901 ctcgcagccg tcgacgcaga cgacgacggt cggctgtttc ggcgccgcat agtttcgttg
      961 gttgacgttg atcatcatgg ggaggttctc tcgagcgcac gcgccttggg cggcgcaggt
     1021 tcggtcgggt gcgcggggtg ctggtggttc ctgacggtgc gttccttgct ctcgatcacg
     1081 tggtcgaaca tcgccttgcc ggccgcgtcc gggtcgcccg aggcgatcgc cttgacgatc
     1141 tggcggtgct cattggccga gatcggcatc agccagccgt cggccaggtt caggcgccgg
     1201 aacaggctca gctccttgat cagcttgcgg tagatcgcgg tcagcttgcc gttgccggcg
     1261 agctcgacca gccggtcgtg gaacttcagg ttcagcaggt ggtagtggtc ggcgtccttc
     1321 gccttcaccg cctgctccat ctgctcgacc aggccgcgga tctctttcaa ctgcaccggc
     1381 gtgatgtgct cggcgaggcg gcggccgacc agctcgtcca tcgcggcgcg caggtcgaag
     1441 atctcgaccg cctcttcgat cgggatgtcg cgcacgaaca cgccgcggtt cttctcggtg
     1501 cgcaccaggc cggcttcttc gagcatgcga aaggcctcgc gcaagggccc gcgcgagacg
     1561 cccaggcgtg ccgccagcgc catctcggtg agtttcgcgc cgggagccaa ttcgcccgag
     1621 agaatcatgc gttcgagctc accctggacc acgctcgtca gcgaactggt ctgcagcagc
     1681 gtgatcgttg gatgtggcac gacggtagcg gtcatgggtg acgagtagat gacaggcctt
     1741 gaagattgtc aacaattttc aagaaacagt tgacgacaga atcgacccac ccctagactg
     1801 actttccagc gacgcgccca accctcgaac gggcacgcgg cttgcaccca ccctcggtcg
     1861 tttttctctt gtcaggagat ctgcatgaat ctgggttcca tcaaaccggt gctcgctgcg
     1921 ctggctttcg tcacggccac cggcgcgttc gcgcagaaga cgcagctcac ggtctacacc
     1981 gcgctcgaga ccgaccagct caaggcctac gaggccggtt tcaccaaggt caaccccgac
     2041 atcgagatca agtgggtgcg cgactcgacc ggtgtgatca ccgccaagct gctcgccgaa
     2101 aaggcgaacc cgcaggccga cgcggtgatg ggcgtggccg cgtcgagcct ggcgctgctc
     2161 gacaagcagg gcatgctgat cccgtacgcg ccgctgaacc tcgacggcat catgagccag
     2221 taccgcgaca agaagaaccc gccggcctgg ttcggcatgg acgtgtgggg cgcgaccgtc
     2281 tgcttcaaca ccgtcgaggc gcagaagaag ggcctgccca agcccgagac ctggaaagac
     2341 ctgaccaagc cgatctacaa gggccagatc gtgatgccga acccggcgtc gtcgggcacc
     2401 ggcttcttcg atgtctcggc ctggctgacg ctctggggcg acaacgacgg caagggcggc
     2461 ggctggaagt acatggacgc gctgcacgag aacatcgcgc agtacaccca ctccggttcc
     2521 aagccctgca acatggccgc gaccggcgag tacgtgatgg gcatctcgtt cgagtaccgc
     2581 ggcaacacca acaaggccaa gggtgcgccg atcgacctga tcttcccgaa ggaa
//
LOCUS       DASRDG010000524         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1004576, whole genome shotgun sequence.
ACCESSION   DASRDG010000524 DASRDG010000000
VERSION     DASRDG010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004576"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFA35_08810"
     CDS             complement(<1..718)
                     /locus_tag="VFA35_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56312.1"
                     /translation="MPASPISAPAAPPRPALALPPRPALRRGLWALPLLISLVFVAAV
                     LAWLQRTEQTDLEEQRGELISDALSLHAQVGARIDQEAALLAAFATHLEQRGLGAAAF
                     ATAPEVQQGLHRFWISLTWLDASNRIVAHVPEQAPGPDLPSLRAAGLSAHLRAPLASG
                     GALIARYSPTEMLRQNVPWWLARKYDVRLVDSFGDVIASTTENRDRGDRQFHRIGLEP
                     ALGDTYLELIARDRVTRWYRS"
     gene            883..1872
                     /locus_tag="VFA35_08815"
     CDS             883..1872
                     /locus_tag="VFA35_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZT56313.1"
                     /translation="MACAIGVLAGCAAGAAWAQAPIVVKFSHVVTPDTPKGKAALRFK
                     QVAEELTQGRVRVEVYPNSQLYKDKEELEALQLGAVQMLAPSLSKFGPLGAKQFEIFD
                     LPYIFQTEAQFVQVTTGKIGQELLASLDNKSIKGLAFWSGGFHVYSANKPLRQPGDLK
                     GLKMRIPSSKVLEASLRTMGALPQTMPFSEVYQALQSGVVDGTEGVPLNFYTQKIYEV
                     QKHMTLSNHGHLAYAVIVNKKFWDGLPADVRGQLEKAMNEATVYSNKIAATENAEGLD
                     EIKKSGKTEIIVPTAAEMAAMRKAMEPLYQDMASRVGKQLIDDVVKATQGVTN"
BASE COUNT          366 a          678 c          726 g          292 t
ORIGIN      
        1 agctgcggta ccagcgcgtc acgcggtcgc gcgcgatcag ctcgaggtag gtgtcgccga
       61 gggctggctc caggccgatg cggtggaact ggcggtcgcc gcggtcgcgg ttctcggtgg
      121 tcgaggcgat cacgtcgccg aagctgtcga ccagccgcac gtcgtacttg cgcgcgagcc
      181 accacggcac gttctggcgc agcatctcgg tcggcgaata gcgcgcgatc agcgcgccgc
      241 ccgaggccag tggcgcgcgc aggtgggccg acaagccggc cgccctcagg ctcggcagat
      301 ccggccccgg ggcctgctcg ggcacgtggg cgacgatgcg gttgctcgcg tccagccagg
      361 tcaggctgat ccagaagcga tgcagcccct gctgcacctc gggcgcggtc gcgaacgccg
      421 cggcgccgag gccgcgctgc tccaggtggg tggcgaatgc ggcgagcagc gcggcctcct
      481 ggtcgatgcg ggcaccgacc tgggcgtgca gcgagagcgc gtcggagatc agctcgccgc
      541 gctgctcttc gagatcggtc tgctcggtgc gctgcagcca ggccagcacc gcggcgacga
      601 acaccagcga gatcagcagc ggcaaggccc acagcccacg ccgcagcgcc gggcgtggcg
      661 gcagcgcgag cgcggggcgc ggcggggcgg ccggcgcgct gatcggtgag gcgggcatgc
      721 gcggaagtct agccagccag ggccgagccg gggctgctgc gcgccgcaca aatgtggaac
      781 tccacaattt cgccgcgcgg ggtcggccgc ccagaatctc gccgcacaag aactcgttcc
      841 agcgacagga gacaagccgt ggctatcctt ctcacgcgcc ggatggcctg cgccatcggc
      901 gtgctcgccg ggtgcgccgc cggcgccgca tgggcgcagg cgcccatcgt cgtcaaattc
      961 agccacgtcg tcacccccga tacgcccaag ggcaaggccg cgctgcgctt caagcaggtc
     1021 gccgaggagc tgacccaggg ccgcgtccgg gtcgaggtct acccgaacag ccagctctac
     1081 aaggacaagg aagagctcga agcgctgcag ctcggtgcgg tgcagatgct ggcgccctcg
     1141 ctgtcgaagt tcggcccgct gggcgcgaag cagttcgaga tcttcgacct gccctacatc
     1201 ttccagaccg aagcgcagtt cgtgcaggtg acgaccggca agatcggcca ggagctgctc
     1261 gcctcgctcg acaacaagag catcaagggc ctggcgttct ggagcggcgg ctttcacgtg
     1321 tactcggcca acaagccgct gcgccagccg ggcgacctga agggcctgaa gatgcgcatc
     1381 ccgtcgagca aggtgctcga agcgagcctg cgcacgatgg gcgcgctgcc gcagacgatg
     1441 ccgttctcgg aggtctacca ggcgctgcag tcgggcgtgg tcgacggcac cgaaggcgtg
     1501 cccttgaact tctacacgca gaagatctac gaagtgcaaa agcacatgac gctgtccaac
     1561 cacggccacc tggcctacgc ggtcatcgtc aacaagaagt tctgggacgg cttgccggcc
     1621 gacgtgcgtg gccagcttga aaaggcgatg aacgaagcga cggtctattc caacaagatc
     1681 gccgcgacgg aaaacgcgga agggctcgac gagatcaaga agtcgggcaa gaccgagatc
     1741 atcgtcccga ctgcggccga gatggccgcc atgcgcaagg ccatggaacc gctctatcag
     1801 gatatggcca gccgcgtcgg caagcagctc atcgacgacg tggtgaaggc gacgcaaggc
     1861 gtcaccaact gataacgttc tgccgggccc cggcctaaga agccggggcc cgttcgcttc
     1921 tcaggggtga ggggattccg gtgtctggtt gcgttcgacg gaagccgcgg gagcggctgc
     1981 cgccgtcaat gacggaattg ccgcccgctg gggcctgcaa caatgctcag gcacgcgagg
     2041 cgtaagtggt tcttcgcatt ct
//
LOCUS       DASRDG010000525         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_882792, whole genome shotgun sequence.
ACCESSION   DASRDG010000525 DASRDG010000000
VERSION     DASRDG010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882792"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFA35_08820"
     CDS             complement(<1..470)
                     /locus_tag="VFA35_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56314.1"
                     /translation="MFRRLVVVALLAVSAALIGYTCWPHEAPLPVDAAAGPSAAAPPA
                     GAPVTPSTVEGALPAPAGRPAAAPAERTEVGHGESTAPDDLPPRDPAFVWIEVRDAGD
                     RRVPGVPVGLRVDDNGHSYLRWRGVTEGNGRVCAPNPTTAPGSRSATAYAQLALP"
     gene            complement(582..>2349)
                     /locus_tag="VFA35_08825"
     CDS             complement(582..>2349)
                     /locus_tag="VFA35_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HZT56315.1"
                     /translation="KEDDAEAQRFRDYFAHDEPLARVAGHRALAMFRARKEGFVDLQL
                     GIGDQREPVATDPTGQGQLLVTTRFAIQHRNRKADAWLADTARLAFRARLALHCESEL
                     FAALRERAETEAIAVFAANLRDLLLAAPAGPRVILGLDPGLRTGCKVAVVDRTGRLLA
                     TTTIYPHAPKDDWDGALTVLGALCQKFAVELVAIGNGTASRETDKLAAELIKKHPAAK
                     LQKVVVSEAGASVYSASERGSEEFPDLDVSLRGAVSIARRLQDPLAELVKIEPKAIGV
                     GQYQHDVDQGQLARALDGVVEDCVNRVGVDLNTASEDLLARVAGLSRTLAKNIVVHRD
                     QHGAFRARRQLRDVSRLGPKAFEQCAGFLRIRGGDDPLDGSAVHPEAYPLVERILARS
                     GRAIDRLLGDCAFLQSLDARQFVDDRFGLPTVTDVLAELEKPGRDPRPEFRTATFQDG
                     VADLGDLRPGMQLEGVVTNVTAFGAFVDVGVHQDGLVHISQLADRFVEDPREVVKAGQ
                     VVTVKVLEVDALRRRIALTMRLFERPGPAAPRTGADRGPRQSGSQPGSQPGPGAHPPR
                     KPEPDAQSPLAAQLAKLKLRKN"
BASE COUNT          288 a          871 c          870 g          320 t
ORIGIN      
        1 ggcagcgcca gctgcgcgta cgcagtggca ctgcgcgatc cgggcgcggt ggtggggttc
       61 ggcgcgcaca cgcggccgtt cccctcggtg accccgcgcc agcgcaggta cgagtggccg
      121 ttgtcgtcga cgcgcagtcc gacggggacg ccgggcacgc ggcggtcgcc ggcgtcgcgc
      181 acctcgatcc acacgaacgc cggatcgcgc ggcggcaggt cgtcgggcgc cgtgctctcg
      241 ccgtgtccga cctcggtgcg ttccgccggc gctgccgcgg gcctgccagc cggcgcgggc
      301 agcgcgcctt cgacggtgct cggcgtcacg ggcgcgccgg caggcggcgc cgcggcagat
      361 ggacccgcgg cagcgtccac aggcaacggc gcttcgtgcg gccagcaggt gtagccgatc
      421 agcgccgccg aaacggccag cagcgcgacc acgaccaggc ggcggaacat cccgcccaac
      481 ataccgcgcg cggcgaccgc gtctgccggg cccacctggg gcggctcgac tcagagaccg
      541 agaaacaatc ccgagtcgat gccgccttcg gcgtgctcga gtcagttctt gcgcagcttg
      601 agcttcgcga gctgcgccgc gagcgggctc tgggcgtccg gttcgggctt gcgcggcggg
      661 tgggcccccg gcccaggctg ggatcccggc tgggaccccg actggcgcgg gccgcggtcc
      721 gcgccggtcc ggggggccgc cggccccggc cgctcgaaga gccgcatcgt cagcgcgatg
      781 cgccggcgca gagcatcgac ctcgagcacc ttcacggtca cgacctggcc ggccttcacg
      841 acctcgcgcg ggtcctcgac gaagcggtcg gcgagctggc tgatgtgcac caggccgtcc
      901 tggtgcacgc cgacgtctac gaacgcgccg aacgcggtga cgttggtcac cacgccttcg
      961 agctgcatgc ccggccgcag gtcgccgagg tcggcgaccc cgtcctggaa cgtcgcggtg
     1021 cggaactccg gccgcgggtc gcggcccggc ttctcgagtt cggcgagcac gtcggtcacg
     1081 gtcggcaggc cgaagcggtc gtcgacgaac tgccgcgcat cgagcgactg caggaacgcg
     1141 cagtcgccga gcagccggtc gatcgcgcgg ccgctgcgcg cgaggatgcg ctcgaccagc
     1201 gggtaggcct cggggtgcac cgccgagccg tcgagcggat cgtcgccgcc gcggatgcgc
     1261 aggaagcccg cgcactgctc gaacgccttc ggcccgagcc ggctgacgtc gcgcagctgc
     1321 cggcgcgcgc ggaacgcgcc gtgctggtcg cggtgtacca cgatgttctt cgccagcgtc
     1381 cgcgacaggc cggcgacgcg cgcgagcagg tcctcgctcg cggtgttgag gtcgacgccg
     1441 acgcggttga cgcagtcctc gaccacgccg tcgagcgcgc gcgcgagctg cccctggtcg
     1501 acgtcgtgct ggtactggcc gacgccgatc gccttgggct cgatcttgac cagctcggcc
     1561 agcggatcct gcagccgccg cgcgatcgac acggcgccgc gcagcgacac gtcgaggtcg
     1621 gggaactcct cgctgccgcg ctcgctcgcc gagtagaccg acgcgcccgc ttcactgacg
     1681 accaccttct gcagtttcgc cgccgggtgc ttcttgatca gctcggccgc gagcttgtcg
     1741 gtctcgcgcg acgcggtgcc gttgccgatc gcgaccagct cgacggcgaa cttctggcac
     1801 agcgcgccga gcaccgtcag cgcgccgtcc cagtcgtcct tcggcgcgtg cgggtagatc
     1861 gtcgtcgtcg cgagcagcct gcccgtgcgg tcgacgaccg cgaccttgca gccggtgcgc
     1921 aggcccgggt cgagtccgag gatgaccctc ggtcccgcgg gcgccgcgag cagcaggtcg
     1981 cgcaggttgg ccgcgaacac ggcgatcgcc tcggtctccg cgcgttcgcg cagcgccgcg
     2041 aacagctcgc tctcgcagtg cagcgccagc cgcgcgcgga acgcgagccg cgccgtgtcg
     2101 gcgagccagg cgtcggcctt gcggttcctg tgctggatcg cgaagcgcgt cgtcaccagc
     2161 agctggccct ggccggtcgg gtccgtcgcg acgggctcgc gctggtcgcc gatgccgagc
     2221 tgcaggtcga cgaagccctc cttgcgggcg cggaacatcg ccagcgcgcg gtggccggcg
     2281 acgcgcgcca gcggctcgtc gtgcgcgaag tagtcgcgga agcgctgtgc ctcggcgtcg
     2341 tcctccttg
//
LOCUS       DASRDG010000526         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1231077, whole genome shotgun sequence.
ACCESSION   DASRDG010000526 DASRDG010000000
VERSION     DASRDG010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231077"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1858
                     /locus_tag="VFA35_08830"
     CDS             <1..1858
                     /locus_tag="VFA35_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZT56316.1"
                     /translation="ALQRLPEARRRAALQTLKLAESLGAVTATLSDSDAAAAAVAYAR
                     EHQLGAIVCGRSAQARAWYAWPRRHFARRVARLAAELELMVVAIEPAQPAGARSPPAD
                     AGSRGHARRWPGYAWALASSGAVTLLATPLRHVFDLANIVMLFLLAVVGIALRFGRGP
                     ALAAALVNVLAFDFFFVAPRFSFAVSDAQYLLTFAVLTIVGLVVGQLTASLRYQARVA
                     REREHRSRRLYEIARELGKALTEAQVAEIGDRSIEAAFDAKAIVLLADAHERLAPASE
                     GVGARPALDIGLAQWAFDHTAPAGTGTDTLPASGQLYLPLLAPIRTRGVIVVEPANPR
                     LLMVPEQRQLLDTYAALIAIAIERVHFVTVAQDTLVSMESERLRNSLLAALSHDLRTP
                     LTALIGGAENLAQALAREGSAHAPDARAIAAQAQRTTQLVDNLLDMARLQAGNVSLRR
                     DWQSLEELAGSALRSVESALAAHPVRIELPNELPLLYADGVLIERVLANLFENAAKYT
                     PAGTKITLGARREGAQIVVDVSDDGPGLPPVEVETLFRKFSRGDKESSTPGVGLGLAI
                     CRAIVQAHGGQISAFDRPAPRHGAVLRFTLPHREMPALAEVDVADAADAPPQ"
     gene            1863..2585
                     /gene="kdpE"
                     /locus_tag="VFA35_08835"
     CDS             1863..2585
                     /gene="kdpE"
                     /locus_tag="VFA35_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HZT56317.1"
                     /translation="MDNSNPARILIVEDDAAIRRFVRQALEAEGHQVFEADGVKRGLI
                     EAGTRKPDLLILDLGLPDGDGVALIRDLRGWSTLPVVVLSARVDETDKIEALDAGADD
                     YLVKPFSVGELLARVRAGLRRRDGAASQASSVVAFGECEIDLARRQVRRRGEHVHLTP
                     VEYKLVAALIGREGRVLTHRQLLGEVWGPNHTEHSHYLRIYLGHLRKKLELDPARPAH
                     FLTETGVGYRFVALPNSGPPAA"
     gene            complement(2582..2920)
                     /gene="fdx"
                     /locus_tag="VFA35_08840"
     CDS             complement(2582..2920)
                     /gene="fdx"
                     /locus_tag="VFA35_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HZT56318.1"
                     /translation="MPIIKILPHSAYCPEGTQIEAPAGTSICEALLDHHIDIEHACEM
                     SCACTTCHVIVRQGLASLGEMDEAEEDLLDRAWGLEPNSRLSCQAILSNQDVVIEIPK
                     YTINHAKENH"
     gene            complement(2943..>4497)
                     /gene="hscA"
                     /locus_tag="VFA35_08845"
     CDS             complement(2943..>4497)
                     /gene="hscA"
                     /locus_tag="VFA35_08845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HZT56319.1"
                     /translation="AMPYALADEGGMVKVRTAAGLKSPVEVSAEILATLRQRAEDSFD
                     DELYGAVITVPAYFDEAQRQATKDAAQLAGLNVLRLISEPTAAAIAYGLENGSEGLYA
                     VYDLGGGTFDISLLRLTAGVFEVVATGGDSALGGDDFDAALAAWALAEAGLDAQTPQD
                     KRAVLVAARAAKEKLSGTDHTTLACPLAGGELAVRVTRAQFEALAQPLLARTLGAVRK
                     VLRDAKTDKSEVRGVVMVGGSTRVPMVQAAVGEFFGQTPLTNLNPDEVVALGAAIQAN
                     ALAGNAGADELLLLDVIALSLGLETMGGLVERIIPRNSTIPTARAQDFTTFKDGQTAI
                     AIHVVQGEREVVSDCRSLARFELRGIPPMVAGAARVRVSFAVDADGLLNVTAREETSG
                     VEAAITVKPSYGLADEEIARMLTESFSSAEADMVERALRESRVEAERMLLATRAALQA
                     DGDLLSADDRARIEALLTALQASREGSDHHAIDAGVEALADGTEAFAAERMNRSIRVA
                     LAGRSVDQI"
BASE COUNT          595 a         1614 c         1623 g          665 t
ORIGIN      
        1 ggccttgcaa cgcctgcccg aggcgcggcg ccgcgccgcg ctgcagacgc tgaaactggc
       61 cgagtcgctc ggcgccgtca ccgccacgct cagcgacagc gatgcggcgg ccgcggccgt
      121 cgcctatgcg cgcgagcacc agctcggcgc gatcgtgtgc gggcgcagcg cgcaggcgcg
      181 cgcctggtac gcgtggccgc gccggcactt cgcgcggcgc gtggcgcggc tggcggccga
      241 gctcgaactg atggtcgtcg cgatcgagcc ggcgcagccc gccggcgcgc gctcgccgcc
      301 cgccgacgcc ggcagccgcg gccacgcccg gcgctggccc ggctacgcct gggcgctcgc
      361 gtcgagcggc gccgtcacct tgctggccac gccgctgcgg cacgtgttcg atctcgcgaa
      421 catcgtgatg ctgttcctgc tcgcggtcgt cggcattgcg ctgcgcttcg gccgtggccc
      481 ggcgctggcc gccgcgctgg tcaacgtgct cgcgttcgac ttcttcttcg tcgcgccgcg
      541 cttctcgttc gcggtgagcg acgcgcagta cctgctgacc ttcgcggtgc tgacgatcgt
      601 cggcctggtc gtgggccagt tgaccgcgtc cttgcgctac caggcacgcg tggcacgcga
      661 gcgcgagcac cggtcgcgcc ggctctacga gatcgcgcgt gagctcggca aggccttgac
      721 cgaggcccag gtcgccgaga tcggcgaccg ctcgatcgag gcggcgttcg acgcgaaggc
      781 gatcgtgctg ctggcggacg cgcacgagcg gctcgcgccc gcctccgagg gcgtcggcgc
      841 gcggccggcg ctcgacatcg ggctcgcgca atgggccttc gaccacaccg cgccggccgg
      901 caccggcacc gacacgctgc ccgccagcgg ccagctctac ctgccgctgc tcgcgccgat
      961 ccgcacgcgc ggcgtgatcg tggtcgagcc ggcgaacccg cgcctgttga tggttcccga
     1021 gcagcgtcag ttgctcgaca cctacgccgc actgatcgcg atcgcgatcg agcgcgtgca
     1081 cttcgtgacg gtcgcgcagg acacgctggt ctcgatggag tcggagcggc tgcgcaactc
     1141 gctgctggcc gcgctctcgc acgacctgcg cacgcccttg accgcactga tcggcggcgc
     1201 ggagaacctc gcgcaggcgc tggcgcgcga gggctcggcc cacgcgcccg acgcgcgcgc
     1261 gatcgccgcg caggcgcagc gcacgaccca gctcgtcgac aacctgctcg acatggcgcg
     1321 gctgcaggcc ggcaacgtga gcctgcggcg cgactggcag tcgctcgaag aactcgccgg
     1381 cagcgcgctg cgctcggtcg agtcggcgct ggccgcgcac ccggtgcgca tcgagttgcc
     1441 gaacgagctg ccgctgctct acgccgacgg cgtgctgatc gagcgcgtgc tcgcgaacct
     1501 gttcgagaac gccgcgaaat acacgccggc gggaacaaag atcacgctcg gcgcgcgccg
     1561 cgaaggcgcg cagatcgtcg tcgacgtgag cgacgacggc ccgggcctgc cgccggtgga
     1621 ggtcgagacg ctgttccgca agttcagccg cggtgacaag gagtcgagca caccgggcgt
     1681 cggcctcggc ttggcgatct gccgcgcgat cgtgcaggcg cacggcgggc agatcagcgc
     1741 gttcgaccgc ccggcgccgc ggcacggcgc ggtgctgcgc ttcacgctgc cgcaccgcga
     1801 gatgccggcg ctggccgagg tggatgtcgc cgatgcagcc gatgcgccgc cacaatgacg
     1861 cgatggacaa cagcaacccg gcccgcatcc tgatcgtcga ggacgacgcg gcgatccgcc
     1921 gcttcgtgcg ccaggcgctc gaggccgagg gccaccaggt gttcgaggcc gacggcgtca
     1981 agcgtggcct gatcgaagcc ggcacgcgca agcccgatct gctgatcctc gacctgggcc
     2041 tgcccgacgg cgacggcgtc gcgctgatcc gcgacctgcg aggctggagc acgctgccgg
     2101 tcgtcgtgct ctcggcgcgc gtcgacgaga ccgacaagat cgaggccctg gacgcaggcg
     2161 ccgacgacta cctggtcaag cccttctcgg tcggcgaact gctcgcgcgc gtgcgcgccg
     2221 ggctgcggcg gcgcgacggt gcggcgtcgc aggcaagcag cgtggtcgcg ttcggcgagt
     2281 gcgagatcga cctcgcccgc cgccaggtgc gccggcgtgg cgagcatgtg cacctgacgc
     2341 cggtcgaata caagctcgtg gcagcgctga tcggccgcga aggccgggtg ctgacgcacc
     2401 gtcaactgct cggcgaagtc tgggggccga accataccga gcacagccac tacctgcgca
     2461 tctacctcgg gcacctgcgc aagaagctgg agctcgaccc ggcaaggccg gcgcacttcc
     2521 tgaccgagac cggcgtgggc tatcgcttcg tcgcgctgcc gaactctgga ccacccgctg
     2581 cctagtgatt ttccttggcg tggttgatcg tgtacttcgg gatctcgatc acgacgtcct
     2641 ggttcgacag gatcgcctgg cacgacaggc gcgagtttgg ctccaggccc caggcgcggt
     2701 cgagcaggtc ttcttcggct tcgtccatct cgccgagcga cgcgaggccc tggcgcacga
     2761 tgacatggca ggtcgtgcag gcgcagctca tctcgcaggc atgctcgatg tcgatgtggt
     2821 ggtcgagcag cgcctcgcag atcgaggtgc cggcgggcgc ttcgatctgc gtgccctcgg
     2881 ggcagtaggc actgtgcggc aggatcttga tgatgggcat gtgaactcgg aaagcgcggg
     2941 gctcagatct ggtcgacgct gcggccggcg agcgcgacgc ggatgctgcg gttcatgcgc
     3001 tcggcggcga aggcctcggt gccgtcggcg agcgcctcga ccccagcgtc gatggcatgg
     3061 tgatcgctgc cctcgcggct ggcttgcagc gccgtcagca gcgcttcgat gcgcgcgcgg
     3121 tcgtcggcgc tcagcaggtc gccgtcggcc tgcagcgcgg cgcgcgtggc cagcagcatg
     3181 cgctcggcct cgacccgcga ttcgcgcagc gcgcgctcga ccatgtcggc ctcggcgctg
     3241 ctgaagctct cggtcagcat gcgcgcgatc tcttcgtcgg ccaggccgta gctcggcttc
     3301 acggtgatcg cggcttcgac gcccgaggtt tcttcgcgcg ccgtcacgtt caacagcccg
     3361 tcggcgtcga ccgcgaagct cacacgcacg cgcgccgcgc cggccaccat cggcgggatg
     3421 ccgcgcagct cgaagcgcgc gagcgagcgg cagtcgctga cgacctcgcg ctcaccctgc
     3481 acgacgtgga tcgcgatcgc ggtctggccg tccttgaagg tcgtgaagtc ctgggcgcgc
     3541 gcggtcggga tcgtgctgtt gcgcgggatg atgcgctcga ccaggccgcc catcgtctcg
     3601 aggccgagcg acagcgcgat cacgtcgagc agcagcagtt cgtcggcgcc ggcgttgccg
     3661 gcgagcgcgt tggcctggat cgccgcgccg agcgcgacga cctcgtcggg gttcaggttg
     3721 gtcagcggcg tttgcccgaa gaattcgccg acggccgcct gcaccatcgg cacgcgggtc
     3781 gagccgccga ccatcacgac gccccgcacc tcggacttgt cggtcttcgc atcgcgcagc
     3841 accttgcgca ccgcgccgag cgtgcgcgcg agcagcggct gcgcgagcgc ctcgaactgc
     3901 gcgcgcgtga cgcgcaccgc gagctcgccg ccggcgagcg ggcacgcgag cgtcgtgtga
     3961 tcggtgcccg agagcttctc cttcgcggcg cgcgcggcga ccagcacggc gcgcttgtcc
     4021 tgcggcgtct gcgcgtcgag tccggcttcg gcgagcgccc aggcggcgag cgcagcgtcg
     4081 aaatcgtcgc cgccgagggc cgagtcgccg ccggtcgcga cgacctcgaa cacgcccgcg
     4141 gtcaggcgca gcagtgagat gtcgaaggtg ccgccgccca ggtcgtagac ggcgtacagc
     4201 ccttcggagc cgttctccag cccgtaggcg atcgcggcag cggtcggttc gctgatcagg
     4261 cgcagcacgt tcaggccggc gagttgcgcc gcgtccttcg tggcctggcg ttgcgcctcg
     4321 tcgaagtagg cgggcacggt gatgacggcg ccgtagagct cgtcgtcgaa gctgtcttcg
     4381 gcacgctgcc gcagggtcgc gaggatctcc gcgctgacct ccaccggcga cttgaggccg
     4441 gcggccgtcc gcaccttgac catgccgcct tcgtcggcga gcgcatacgg catcgcg
//
LOCUS       DASRDG010000527         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_439728, whole genome shotgun sequence.
ACCESSION   DASRDG010000527 DASRDG010000000
VERSION     DASRDG010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439728"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..313
                     /locus_tag="VFA35_08850"
     CDS             95..313
                     /locus_tag="VFA35_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56320.1"
                     /translation="MNWAHGFSWLAALTAPRGEQHAHDCAEMGTAFGLEASMDEHPAG
                     EPEPGCRAPAPASPAEAWQRRVVRRSGL"
     gene            complement(314..1084)
                     /locus_tag="VFA35_08855"
     CDS             complement(314..1084)
                     /locus_tag="VFA35_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZT56321.1"
                     /translation="MRLQLLSDLHLETETFEPTPAPDAELLVLAGDIDSSWAGLERFR
                     DWPVPVIFVAGNHEFDGRELTQAWPALRERCVRLGIRLLECESLVVPDRAGRRIRFVA
                     TVRWCDFDLFGPLLRERAMRAGRYFVKVMRSTRHGAPFDAAAVREESLACRDWLHGEL
                     TRPRGDWDKTVVVTHFAPSLRSADPRYGAQPGTASFCNADDALLPLADLWLHGHLHWR
                     FDYLVPHAGGATRVVCNARGLARKGEADGYPPVWVLEL"
     gene            complement(1084..1857)
                     /locus_tag="VFA35_08860"
     CDS             complement(1084..1857)
                     /locus_tag="VFA35_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HZT56322.1"
                     /translation="MTPKEAPPSTAARAHDLTDAEFAELDELLLQTPEPLEPLDAVML
                     DGYLCGVIVQPVLLEPATWLAHVFDFDGRPLPDDVNPEWLARTTALIMRRHAALNRAI
                     VEDGWFDPLVLEFDDEHPREPVPEGEIDPLAGLSPVSQALMPWVAGFHHAAETFPDLA
                     EMPDDAVMAALARLYRHLPVEDDQQRELVALLDRERPLSDLDDAMEELVVTVADLQDL
                     TAEQRYKVETVKRETPKVGRNDPCPCGSGKKFKQCHGAS"
BASE COUNT          286 a          684 c          756 g          276 t
ORIGIN      
        1 ccaagtgcga ccgggaaggg cctcatttcg atgcaccggg cgccgataac gggctcacac
       61 tgagatacat cacaaggtca cgcgggaggt cgcgatgaac tgggcacacg gtttttcatg
      121 gttggcggcg ctcacggcgc cgcgcggcga gcagcacgcg cacgactgcg ccgagatggg
      181 caccgcgttc gggctggagg ccagcatgga cgagcacccg gcgggcgaac ccgaacccgg
      241 ttgtcgcgcg cctgcgccgg cctcgcccgc cgaggcgtgg cagcgccgcg tggtgcggcg
      301 ctccggcctt tgatcagagc tcgagcaccc acacgggcgg gtagccgtcg gcctcgccct
      361 tgcgcgccag gccgcgcgcg ttgcagacga cgcgggtcgc gccacccgcg tgcggcacca
      421 ggtagtcgaa ccgccagtgc aggtggccat ggagccacag gtcggccagc ggcagcagcg
      481 catcgtcggc gttgcagaag ctcgcggtgc cgggctgcgc gccgtagcgc gggtcggcgc
      541 tgcgaaggct gggcgcgaag tgggtgacga cgacggtctt gtcccagtcg ccgcgcggcc
      601 gcgtgagctc gccgtgcagc cagtcgcggc aggcgagtga ctcttcgcgt accgcggcgg
      661 cgtcgaacgg cgcgccgtgg cgcgtcgagc gcatcacctt gacgaagtag cggccggcac
      721 gcatcgcgcg ttcgcgcagc aaagggccga acaggtcgaa gtcgcaccag cgcacagtgg
      781 cgacgaagcg gatgcgccgg ccggcgcggt cgggcacgac caggctctcg cattcgagca
      841 gccggatgcc gaggcgcacg cagcgctcgc gcagcgcggg ccaggcctgc gtgagctcgc
      901 gcccgtcgaa ctcgtggttg ccggcgacga agatcaccgg caccggccag tcgcggaacc
      961 gctccagccc ggcccagctg ctgtcgatgt cgccggcgag cacgagcagt tcggcgtcag
     1021 gcgccggcgt cggctcgaag gtttcggttt cgaggtgcag gtccgacagc agctgcagcc
     1081 gcatcagctc gcgccatggc actgcttgaa cttcttgccg ctgccgcacg ggcacgggtc
     1141 gttgcggccg accttgggcg tttcgcgctt cacggtctcg accttgtagc gctgctcggc
     1201 ggtcaggtcc tgcaggtcgg cgaccgtcac gacgagctcc tccatcgcgt cgtcgaggtc
     1261 gctgagcggg cgctcgcggt cgagcagcgc gaccagttcg cgctgctggt cgtcctcgac
     1321 cggcaggtgg cggtacaggc gcgcgagtgc ggccatcacc gcgtcgtcgg gcatctccgc
     1381 gaggtcgggg aaggtctcgg cggcgtggtg gaagccggcg acccagggca tcagcgcctg
     1441 cgacaccggg ctcaggccgg ccagcgggtc gatctcgcct tcgggcaccg gctcgcgcgg
     1501 gtgctcgtcg tcgaactcga gcaccagcgg gtcgaaccag ccgtcctcga cgatcgcgcg
     1561 gttcagcgcg gcgtggcggc gcatgatcag cgcggtcgtg cgggcgagcc actcggggtt
     1621 cacgtcgtcg ggcagcggcc ggccgtcgaa gtcgaacaca tgggcgagcc aggtcgcggg
     1681 ctcgagcagc accggctgca cgatcacgcc gcacaggtag ccgtcgagca tcaccgcgtc
     1741 gagcggttcc agcggctcgg gggtctggag cagcagctcg tcgagctcgg cgaactcggc
     1801 gtcggtcagg tcgtgggcgc gcgcggcggt cgacgggggg gcttctttcg gggtcatggc
     1861 ccgattgtga tcgctctaaa ccgagaagcg cccgatcagc cggtagcgcg cgccggggtg
     1921 atacaggcgc gcgaccgacg cgaccgtgcc ctgcgacacg gtgcggcgcg acaccatcag
     1981 cagcgcgctg cccgcggcca cg
//
LOCUS       DASRDG010000528         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_440269, whole genome shotgun sequence.
ACCESSION   DASRDG010000528 DASRDG010000000
VERSION     DASRDG010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440269"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..785
                     /locus_tag="VFA35_08865"
     CDS             42..785
                     /locus_tag="VFA35_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HZT56323.1"
                     /translation="MTTLLNPPLTEAAQLDAALRERGHAVLSAAGLRALLPASAAELD
                     ALRPSWADLPPDEHMRDGGRYRRRRHACFVVEGRGVAPVPHRAHWQPLEYNALHGGLE
                     RWFEPMLPEVAAQPVWSRLLVALSAHCSALKGVQPWFVEAHQFRIDTTDGIGRPTPEG
                     AHRDGVDFVAVLLVDRQGIKGGETRVFDADGPAGLRFTLTEPWTTLLLDDARVIHEST
                     PIQPLAPGGHRDTLVLTFRAGGFQGPAAT"
     gene            complement(818..1522)
                     /locus_tag="VFA35_08870"
     CDS             complement(818..1522)
                     /locus_tag="VFA35_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56324.1"
                     /translation="MGGTIPGRADPSFILLAIRSMGSTDSKVQYDRMLVYGPDLAASS
                     AISGYIYAGGDGWCSSPDPTTYNGRDQGRRAGEPVYLRTTADARAPMVSGSVRYRAAT
                     YALSGGPLPGVASGYTFAQPANIADIAGDWKLTDGAGNAFALTVGSSGSVFGSYRGCD
                     LSGALQPDPTGVNQFALQFRVASGGCSSLQGHAGYPYQGPILAYPLAAGGWQMLMWAE
                     WTDGWDWDGLLAVGRR"
     gene            complement(1821..2327)
                     /locus_tag="VFA35_08875"
     CDS             complement(1821..2327)
                     /locus_tag="VFA35_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56325.1"
                     /translation="MLKLTSLKSAALVSVTAAATTLLIQACGGGDAVAQSASDADVVE
                     GVWDSTVTNKDCTSGAVLGAPFKALIVFRRGGTFDADNAQGRALRGNIYGIWKHGAGT
                     AYTANAVHQRFNPDGTYAGTNKVQRSLTLAADASTFTSTLAVQMLDTSGNVVGQACPT
                     ETAVRMNL"
     gene            2451..>3138
                     /locus_tag="VFA35_08880"
     CDS             2451..>3138
                     /locus_tag="VFA35_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZT56326.1"
                     /translation="MRAAERYAFGTFVLERSQRRVLRSDGVVVDLTPRLFSALLMFVE
                     QPGDLLDKDALMRALWPGLVVGENSLSQVISGLRRALGDEPQGSRYIQTVPRHGFRFV
                     AKVSPLPAEVTQPAADSEIPLPAGGATRRPARRRWLGATLALGAAAGIGGAGWWGWRR
                     SASVSAAGHQPTLAVLPFKPLVAEGRDELLELGMADSLIAHLSTVPGLVVRSSGSVRR
                     YAGAEQDPLRA"
BASE COUNT          444 a         1094 c         1119 g          481 t
ORIGIN      
        1 ggcggtccgg cgcggtcgca tctcacggca tcattcgggg catgacgacc ctgctgaatc
       61 cgccactcac cgaagcggcg caactcgacg ccgcgctgcg cgagcgcggc cacgccgtgc
      121 tgagcgccgc cggcctgcgc gcgctgctgc ccgcctcagc cgccgaactc gatgcgctaa
      181 ggccgagctg ggccgacctg ccgcccgacg aacacatgcg cgatggcggt cgctaccggc
      241 gccggcgcca cgcctgtttc gtcgtcgagg gccgcggcgt cgcgccggtg ccgcaccgcg
      301 cgcactggca gccgctcgag tacaacgcgc tgcacggcgg gctcgagcgc tggttcgagc
      361 cgatgctgcc ggaggtcgcg gcgcagccgg tctggtcgcg cctgctcgtg gcgctgagtg
      421 cgcactgctc ggcgctgaag ggcgtgcagc cctggttcgt cgaggcgcat cagttccgca
      481 tcgacaccac cgacggcatc ggccggccga cgcccgaggg cgcgcaccgc gacggcgtcg
      541 acttcgtcgc ggtgctgctg gtcgaccggc aaggcatcaa gggcggcgag acgcgcgtgt
      601 tcgacgccga cggcccggcc ggcctgcgct tcacgctgac cgagccctgg accacgctgc
      661 tgctcgacga cgcccgtgtg atccacgaga gcacgccgat ccagccgctc gcgccgggcg
      721 gccaccgcga cacgctggtg ctgaccttcc gcgcgggcgg gtttcagggg ccggccgcga
      781 cctgatcgac ctcgtcaacg ctggccttgc gtgggtgtca gcggcggccg acggcgagca
      841 ggccatccca atcccagccg tcggtccact cggcccacat caacatctgc catccgccgg
      901 ccgccagcgg ataggccaag atggggccct ggtaggggta tcccgcatgt ccctgcaagg
      961 aagaacagcc gccggaagcg actcgaaact gcaaggcgaa ctggttcacg cccgtcggat
     1021 caggctgcag cgcgccgctc aggtcgcatc cgcggtagct cccgaagaca gagccgctgc
     1081 tgcccacggt cagcgcgaag gcatttccgg cgccatcggt cagcttccag tcacccgcaa
     1141 tgtcggcgat gtttgcgggc tgcgcgaacg tgtagccgct tgcgaccccg ggcagcgggc
     1201 cgccgctcaa ggcataggtg gcagcgcgat atctgaccga gcctgacacc atcggtgccc
     1261 gggcgtccgc tgtcgtacgc aagtagacgg gttcgcccgc acggcgcccc tggtcacgcc
     1321 cgttgtatgt cgtcggatcg gggctgctgc accagccatc gccgccggcg tagatatagc
     1381 cgctgatggc cgacgaggcc gccaggtcgg gcccatagac cagcatccgg tcgtattgga
     1441 ccttggagtc tgtcgacccc atgctgcgga tggcgaggag aatgaacgac ggatcggccc
     1501 gaccggggat cgtgccgccc acggcggcag gcccgctcgc cgcgggctga gggccttccg
     1561 gcggtggcgt gcaggcgaac gcggttgccg caggcgcagg cgcaggagag gccgccgccg
     1621 gtgcggccac taccggcgca tcggttccgc cgccaccgca tgcggccagc aacgcggcca
     1681 gcgcgagcgc gggccccgtg ggaacgggag ccggcgcgcg gcggcgctgc cctcgcgcaa
     1741 gaatcgatcg cctcgtcatg accgttctcc gatcgagggt gtgcgtggca cggatgcgta
     1801 catgtcgcct ccccgcggga tcaaaggttc atgcggaccg cggtctcggt gggacaggcc
     1861 tgtccgacga cgttgccgct ggtgtcgagc atctgcaccg ccagggtgct cgtgaaggtg
     1921 ctggcgtccg ccgccagagt caggctgcgc tggaccttgt tcgtcccggc gtaggtgccg
     1981 tccgggttga atcgctggtg cacggcgttg gccgtgtacg ccgtgccggc gccgtgtttc
     2041 cagatcccgt agatgttgcc gcgcagggct cttccctgcg cgttgtcggc gtcgaacgtg
     2101 ccgcctcgcc tgaacacgat cagggccttg aacggcgcac ccaggacggc gccgctcgtg
     2161 cagtccttgt tggtgaccgt ggaatcccag accccttcga cgacatccgc gtcgctggcc
     2221 gactgcgcca ccgcgtcgcc gccgccgcat gcctggatca atagcgtggt ggccgcggcc
     2281 gtgacactga cgagtgcggc cgatttgagc gatgtgagtt tgagcatgat gcctccgcag
     2341 agtgattgat gaagcggccg caggctgcga ccgtcggggc acaatagggc ggcgatcgcc
     2401 gtaagtcctg agccggccct gaaggattcc tgaaagagct tgaaatcgtg atgcgcgcag
     2461 cggaacgcta tgcctttggc accttcgtgc tcgaacggtc ccagcggcgc gtgctgcgga
     2521 gcgacggcgt cgtggtcgac ctgaccccac ggctgttcag cgccttgctg atgtttgtcg
     2581 agcaaccggg agacttgctc gacaaggacg cgctgatgcg ggccttgtgg ccggggctgg
     2641 tggtcggcga aaacagcctg agccaggtca tctcgggcct gcgacgcgca ctcggtgacg
     2701 agccccaggg tagccgctac atccagaccg taccgcgcca cggcttcagg ttcgtggcaa
     2761 aggtttcgcc gttgcccgcc gaggtgacgc agcccgccgc cgacagcgag atccccctac
     2821 cggcgggcgg tgccacgcgc cggcccgcca ggcgccgttg gttgggggca acgctggctc
     2881 tcggcgcggc cgccggcatc ggtggcgcag gctggtgggg ctggcgccgg tccgcctcgg
     2941 tgagcgctgc aggccaccag cccacgctgg ccgtgctgcc gttcaagccg ctcgtggccg
     3001 aggggcgcga cgagttgctc gaactcggca tggccgacag cctgatcgcc catctctcga
     3061 cggtgcccgg cctggtggtg cgctcgagcg gctcggtgcg gcgctacgcc ggcgctgagc
     3121 aggatccgct gcgcgcgg
//
LOCUS       DASRDG010000529         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_311207, whole genome shotgun sequence.
ACCESSION   DASRDG010000529 DASRDG010000000
VERSION     DASRDG010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311207"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /gene="phaZ"
                     /locus_tag="VFA35_08885"
     CDS             <1..1089
                     /gene="phaZ"
                     /locus_tag="VFA35_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HZT56327.1"
                     /translation="DLDVLTRLKEQPTVLVVAPLSGHHSTLLRETVRELLRYHKVFIT
                     DWTDARMVPKDAGPFHLDDYIAYVQEFIRHIGPEANVISVCQPTVPVLAAISLMASRG
                     EVTPRTMTMMGGPIDARKSPTAVNNLAINKPYEWFENNVIYRVPTNFPGSGRAVYPGF
                     LQHTGFVAMNPDRHMSSHYDYFRDLIRGDDDSVEFHRGFYDEYNAVLDMPAEYYLDTI
                     KTVFQDFALVNGTWEVSGELVRPQDIKTTALLTIEGELDDISGAGQTRAAHDLCTGIP
                     KDRQFHYDVIGAGHYGIFSGRRWREKVYPKVRDFIASYQPAAAAAKPKAAARKATVVK
                     PATSQLALTLVPKATAKPVAKKAARKAK"
     gene            1086..1718
                     /gene="rsxB"
                     /locus_tag="VFA35_08890"
     CDS             1086..1718
                     /gene="rsxB"
                     /locus_tag="VFA35_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HZT56328.1"
                     /translation="MNASGLVQALDDALPQTQCTRCGYPDCRSYAQAIVAGEAEINQC
                     PPGGDAGIVRLAALTGRPALPLSPAHGTEAPRQLAVIDETWCIGCTLCLKACPVDCIV
                     GGPKAMHTVIDAQCTGCELCLPACPVDCIALVPATGTRTGWNAWSAAQADEARTRHAW
                     HRSRLERDARAQLQRLAAGGHAAASSAAGAPAKRSVVEAALARARAARAA"
     gene            1757..>2312
                     /gene="nth"
                     /locus_tag="VFA35_08895"
     CDS             1757..>2312
                     /gene="nth"
                     /locus_tag="VFA35_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZT56329.1"
                     /translation="MKTQDIEPFFAALKAANPAPETELEYTNVFELLAAVLLSAQATD
                     VGVNKATRRLFPIANTPAKMLALGLDGLTEHIRTIGLYRTKAKNLLETCRLLIARHGG
                     QVPGTREALEALPGVGRKTANVVLNVAFGAPTMAVDTHIFRVSNRTGLAPGKNPLEVE
                     LKLLERVPAAYLVDAHHWLILHGRY"
BASE COUNT          391 a          855 c          751 g          315 t
ORIGIN      
        1 gacctggacg tgctcacgcg cctgaaggag cagccgaccg tgctggtcgt ggcccctctc
       61 tcgggccacc actcgacgtt gctgcgcgag accgtgcgcg agctgctccg gtaccacaag
      121 gtcttcatca ccgactggac cgacgcgcgc atggtgccga aggacgccgg cccgttccac
      181 ctcgacgact acattgccta tgtgcaggag ttcatccgcc acatcggccc cgaggcgaac
      241 gtgatctcgg tgtgccagcc gaccgtgccg gtgctcgcgg cgatctcgct gatggccagc
      301 cgcggcgagg tcacgccgcg cacgatgacg atgatgggcg gcccgatcga cgcccgcaag
      361 agcccgacgg ccgtcaacaa cctcgcgatc aacaagccct acgagtggtt cgagaacaac
      421 gtgatctacc gcgtgccgac gaactttcca ggcagcgggc gcgcggtgta cccgggcttt
      481 ctgcagcaca ccggcttcgt cgcgatgaac ccggatcgcc acatgagctc gcactacgac
      541 tacttccgcg acctgatccg cggtgacgac gacagcgtcg agttccaccg cggcttctac
      601 gacgagtaca acgccgtgct cgacatgccg gccgagtact acctcgacac gatcaagacc
      661 gtgttccagg atttcgcgct cgtcaacggc acctgggagg tgagcggtga actcgtccgg
      721 ccgcaggaca tcaagaccac cgcgctgctg acgatcgaag gcgagctcga cgacatctcc
      781 ggcgccggcc agacccgcgc tgcgcacgat ctgtgcaccg gtatcccgaa ggaccgccaa
      841 ttccactacg acgtgatcgg cgccggccac tacggcatct tctcgggccg ccgctggcgc
      901 gagaaggtct acccgaaggt gcgcgatttc atcgcgagct accagccagc cgccgcagcg
      961 gcgaaaccga aggccgcggc gcgcaaggcc accgtggtca agccggcgac gagccagctc
     1021 gcgctgacgc tggtgccgaa ggccaccgcc aagccggttg cgaagaaggc cgcgcgcaag
     1081 gcgaagtgaa cgcttccggg ctcgttcagg cgctcgacga tgcgctgccg caaacgcagt
     1141 gcacccgttg cggctacccc gattgccgca gctatgcgca ggcgatcgtg gccggcgagg
     1201 ccgagatcaa ccagtgccca ccgggcggtg acgccggcat cgtgcgcttg gccgccctca
     1261 ccggccggcc ggcgctgccg ctgagcccgg cgcacggcac cgaggcgccg cgccagctcg
     1321 cggtgatcga cgaaacctgg tgcatcggct gcaccctgtg cctgaaagcc tgcccggtcg
     1381 actgcatcgt cggcggcccc aaagccatgc acaccgtgat cgacgcgcaa tgcaccggct
     1441 gcgagctgtg cctgccggcc tgcccggtcg actgcatcgc cctcgtaccg gccaccggca
     1501 cacgcaccgg ctggaacgct tggagcgccg cccaggcgga cgaagcgcgc acgcgccacg
     1561 cctggcatcg ctcccggctc gagcgcgatg cgcgcgcgca gttgcagcgc ctcgccgcgg
     1621 gcgggcacgc ggccgcatcg agcgccgcgg gcgcaccggc caagcgctcg gtggtcgaag
     1681 ccgccctcgc gcgcgcccgc gccgcccgcg cggcctgacc tccctcggtg gcgcacgcga
     1741 ggccacaatc gcgacgatga agacgcagga catcgaaccc ttcttcgcgg cgctgaaggc
     1801 cgcgaacccg gcgcccgaga ccgagctcga gtacaccaac gtgttcgagc tgctcgctgc
     1861 cgtgctgctc tcggcgcagg cgacggatgt cggcgtcaac aaggcaacgc gccggctgtt
     1921 cccgatcgcc aacacgccgg ccaagatgct ggcgctcggc ctcgacggcc tgaccgagca
     1981 catccgcacg atcggcctgt accgcacgaa ggcgaagaac ctgctcgaga cctgccgcct
     2041 gctgatcgcg cggcacggcg gccaggtgcc cggcacccgc gaggcgctcg aagccctgcc
     2101 cggcgtcggc cgcaagaccg cgaacgtcgt gctgaacgtg gctttcggcg caccgacgat
     2161 ggcggtcgac acgcacatct ttcgggtctc gaaccgcacc ggcctggcgc cgggcaagaa
     2221 cccgctcgag gtggagttga agctgctcga gcgcgtgccc gcggcctacc tcgtcgacgc
     2281 ccaccactgg ctgattttgc atggccgcta cg
//
LOCUS       DASRDG010000530         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1232166, whole genome shotgun sequence.
ACCESSION   DASRDG010000530 DASRDG010000000
VERSION     DASRDG010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232166"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VFA35_08900"
     CDS             <1..799
                     /locus_tag="VFA35_08900"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZT56330.1"
                     /translation="TRVVLGLRDVLDAPVRARAELARNDTVGVIRASYDEVLVYGQRE
                     VFDVASEFGLPADIAASIRYVGAVVPPETRAPVAPGNGRLRVLVTAGGGEDGYDLLRW
                     AMAAVRGPMRASDLAVTLVTGPLLADAAFAELERAVQGDPRCTLLRVSRAMHELTDKA
                     DLVVGMGGYNTVYETLARGLPLLALPRRGPRLEQWERCRRLAALGALTLLDHGDARDP
                     ELFAAAMREAAGRKADLSLLAFDGAERTADHCLAPRTAVRALPMTAH"
     gene            807..2033
                     /locus_tag="VFA35_08905"
     CDS             807..2033
                     /locus_tag="VFA35_08905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZT56331.1"
                     /translation="MHVGYVLKKFPRNSETFILNEILALQRAGVQVSAFSLNKPDDGV
                     FHRALADLGSPITYLQPRRPDAWLELMRGHWPRISRLLPRLLAEFEDLLLGGRPDLWT
                     ILGHGFDLALLLEQRGVQRMHAHFATVAAYVARAANAFTGVPFSVTCHAKDIYREAVT
                     ADRFTALLRPADFAVTVCEANRRHIVDNLGGAALDVRVLYNGVDVAAFHPRDRRPDPQ
                     PTILAVGRLVEKKGFHVLLDALSLLAQQGQRPRCWLVGDGEERERLQAQAQTLGLQNV
                     EFLGMRTQDEVRGYMARATVMALPCIVGADGNRDALPTVLLEALAAGLPTISSPVGGV
                     EEIADHGRAGVLVEPGSAAATARAIHDLLRDPARQAALARAGRAHAEQCFDLTTNVAR
                     LRAWYENGARAPGAVA"
     gene            2030..>2371
                     /locus_tag="VFA35_08910"
     CDS             2030..>2371
                     /locus_tag="VFA35_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56332.1"
                     /translation="MTAGRVAYLCADPGIPPDGSKGASVHFRAMAAALLRSGVDLDVF
                     MTREGDASVFAPHRARVVPTPRARGVAGEVLQLGHSGALLEALAGAGPHVAVYERLSL
                     FAAAGLAHARAL"
BASE COUNT          286 a          893 c          875 g          317 t
ORIGIN      
        1 cacgcgcgtc gtgctcggcc tgcgcgacgt gctcgacgcg ccggtgcgcg cgcgcgcgga
       61 gctggcccgc aacgacaccg tcggcgtgat ccgcgccagc tacgacgaag tgctcgtcta
      121 cggccagcgc gaggtgttcg acgtcgcctc cgagttcggc ctgccggcgg acatcgctgc
      181 gtccatccgc tacgtcggcg ccgtcgtgcc gccggagacg cgcgcgccgg tcgcccccgg
      241 gaatgggcgg ctgcgggtgc tcgtcacggc gggcgggggc gaggacggct acgacctgct
      301 gcgctgggcg atggcggccg tgcgcggccc catgcgcgcg agcgacctcg cagtgaccct
      361 tgtcaccggc ccgttgctgg cggacgccgc gttcgccgaa ctggaacgtg cagtccaggg
      421 agatccgcgg tgtactctcc tacgggtgtc gcgcgccatg cacgagctca cggacaaggc
      481 cgacctcgtg gtcggcatgg gcggctacaa caccgtgtac gagaccctcg cgcgcggtct
      541 gccgctcctc gcgctgccgc gccgcggccc gcgcctcgag cagtgggaac gctgccgccg
      601 cctcgccgcg ctcggcgcgc tcacgctgct cgaccacggc gacgcgcgcg acccggagct
      661 cttcgcggcg gcgatgcgcg aagcggccgg ccgcaaggcc gacctctcgc tgctcgcgtt
      721 cgacggcgcc gagcgcacgg ccgaccactg cctcgccccg cgcaccgccg tgcgcgcgct
      781 gcccatgacc gcgcactgag gtccacgtgc acgtcggcta cgtcctcaag aagttcccgc
      841 gcaactcgga gaccttcatc ctgaacgaga tcctggcgct gcagcgcgcc ggcgtgcagg
      901 tgagcgcctt ctcgctcaac aagccggacg acggcgtgtt ccaccgcgcg ctggccgacc
      961 tcggcagtcc gatcacctac ctgcagccga ggcggcccga cgcatggctc gagctgatgc
     1021 gcgggcactg gccgcgcatc tctcgcctgc tgccgcggct gctggcggag ttcgaggacc
     1081 tgctgctcgg cggccgcccc gacctgtgga ccatcctcgg ccacggcttc gacctggcgc
     1141 tgctgctcga gcagcgcggc gtgcaacgca tgcacgcgca cttcgcgacc gtcgcggcct
     1201 acgtcgcgcg cgcggccaac gcgttcacgg gcgtgccgtt cagcgtcacg tgccacgcga
     1261 aggacatcta ccgcgaagcc gtgacggcgg atcggttcac ggcgctgctg cggccggcgg
     1321 acttcgcggt gacggtgtgc gaggccaaca ggcgccacat cgtggacaac ctcggcggcg
     1381 ccgccctcga cgtgcgcgtg ctctacaacg gcgtcgacgt cgcggcgttc cacccgcgcg
     1441 atcgcagacc cgacccgcag ccgacgatcc tcgcggtcgg ccggctggtg gagaagaagg
     1501 gcttccacgt gctgctcgat gcgctgtcgc tgctggcgca gcagggccag cgcccgcgct
     1561 gctggctggt cggcgacggc gaggagcgcg agcgcctgca ggcgcaggcg cagacgctcg
     1621 gcctgcagaa cgtcgagttc ctcggcatgc gcacgcagga cgaggtgcgc ggctacatgg
     1681 cgcgcgcgac ggtgatggcg ctgccgtgca tcgtcggcgc cgacggcaac cgcgacgcgc
     1741 tgccgaccgt gctgctcgag gcgctcgccg cgggcctgcc gaccatcagc tcgccggtgg
     1801 gcggcgtcga ggagatcgcc gaccacgggc gcgccggcgt gctcgtcgaa cccggctcgg
     1861 cggcggcgac cgcgcgcgcg atccacgatc tgctgcgcga tccggcgcgg caggcggcgc
     1921 tggcgcgggc cggccgcgcg cacgccgagc agtgcttcga tctgacgacc aacgtggcgc
     1981 ggctgcgcgc gtggtacgag aacggcgcgc gcgcccccgg ggcggtggcg tgaccgcagg
     2041 ccgcgtcgcc tacctctgcg cggatcccgg catcccgccg gacggcagca agggcgcctc
     2101 ggtgcacttc cgtgcgatgg cggcggcgct gctgcgcagc ggcgtcgacc tcgacgtgtt
     2161 catgacccgc gaaggcgacg catcggtgtt cgcgccgcac cgtgcgcgcg tggtgccgac
     2221 gccccgcgcg cgcggcgtcg ccggcgaggt gctgcagctc ggacattcgg gcgcgctgct
     2281 ggaggcgctg gccggcgcgg ggccgcacgt cgccgtctac gaacggctgt cgctgttcgc
     2341 ggccgccggg ctcgcgcacg cgcgcgcgct c
//
LOCUS       DASRDG010000531         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1232532, whole genome shotgun sequence.
ACCESSION   DASRDG010000531 DASRDG010000000
VERSION     DASRDG010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232532"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1338)
                     /locus_tag="VFA35_08915"
     CDS             complement(13..1338)
                     /locus_tag="VFA35_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HZT56333.1"
                     /translation="MAQAIVELKNVGKSFKSADGSARSVLEGVDFRLAEGEIVALLGQ
                     SGSGKSTMLRIMAGLIGADSGEVRLRDQPLYGPARGVSMVFQSFALFPWLTVQANVEL
                     GLEARGIAPAERARRAEAAIKLIGLTGFEGALPRELSGGMRQRVGIARALVVEPEVLL
                     MDEAFSALDVLTGERLREDILELWTGKAMPTKAILVVSHNIEEAVLMADRVLIFASDP
                     GRVRFQLAVQLPRPRNPESAEVRQLIDEVYALMTVGRASKGRAAEEPAALRLAERLPE
                     ADVARMEGLLELLAGDTFDGRADLPKLAEETELTDDELLPLAQAVALLGFAQLAQGDL
                     HLTPLGRRYVEGEHALRQGLFGQQLLAHVPLAAHIRHSLEQEASGELPEEPFLRLLSE
                     TLDAAEAERVLRTAIEWGRHGEVFEYNYTTGLIHLPEGEAGSEPRKADA"
     gene            complement(1356..3083)
                     /locus_tag="VFA35_08920"
     CDS             complement(1356..3083)
                     /locus_tag="VFA35_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZT56334.1"
                     /translation="MIDKRFFPRQVVESAGNRWDWALLPLVLGVFALLAWGGSQMARP
                     YQLGQALPLTLDPTHLPYYLLRTTLRMFLALAASLVFACVFAAVAAKYRAAERVMVPL
                     LDILQSIPILGFLSITVTGFIALFPGNLFGVECAAIFAIFTSQAWNMAFSLYQSLKTV
                     PAELQEAAAVFQLSGWQRFWRLELPFAMPGLLWNMMMSMSGGWFFVVASEAISVANQS
                     IKLPGVGSYIALAIEQRDLRAIGWAIGGMLVGIVLYDQLFFRPLLAWADKFRFEEGSN
                     DTAPTSWLLTWMRRTDALSALAMRFVRLLERSLLLFRRSHDGTSIRARTAPPNPAWSR
                     VWDAAVAAAVLLAVWQLVAFIRAEVGFAEVLHVFVLGSYTMLRVIVLIALAALVWVPI
                     GVWIGMNPRWAGRLQAAAQFLAAFPANLMFPVAVVFIVTWHLNPNLWLSPLMVLGTQW
                     YLLFNVIAGASTVPTELRYAAKNLGLRGWLKWRRYLLPAVFPSFVTGAITASGGSWNA
                     SIVAEYVTWGDTTLQAQGLGSYIAHMTAIGDFPRIALGIGVMCVFVMGMNHFLWRRLY
                     RMAEDRMHF"
BASE COUNT          521 a         1075 c         1068 g          422 t
ORIGIN      
        1 tgcgatgcag ggtcatgcgt cggcctttcg gggctcagag ccggcctcgc cttcgggcag
       61 atggatcagc ccggtcgtgt agttgtattc gaacacctcg ccgtgccggc cccactcgat
      121 cgcggtgcgc agcacgcgct cggcctcggc ggcgtcgagc gtttcgctga gcaggcgcag
      181 gaacggctcc tcgggcaatt cgcccgaggc ctcctgctcg aggctgtggc ggatgtgcgc
      241 ggcgagcggc acgtgcgcga gcagttgctg gccgaacagc ccctggcgca gcgcgtgctc
      301 gccctcgacg tagcgccggc ccagcggcgt gaggtgcagg tcgccctgcg cgagctgcgc
      361 gaagccgagc agcgcgacgg cctgcgccag cggcagcagt tcgtcgtcgg tgagctcggt
      421 ttcttcggcg agcttgggca ggtcggcgcg gccgtcgaag gtgtcgccgg caagcagctc
      481 cagcaggccc tccatccgtg cgacgtcggc ctcgggcaga cgttcggcga gccgcagcgc
      541 ggccggctcc tcggcggcgc ggcccttgct cgcgcggccc accgtcatca gcgcatagac
      601 ctcgtcgatc agctgccgca cctcggcgct ctcggggttg cgcggccgcg gcagctgcac
      661 cgcgagctgg aagcgcacgc gccccgggtc gctcgcgaag atcagcacgc ggtcggccat
      721 cagcacggcc tcctcgatgt tgtgcgagac gaccaggatc gccttggtcg gcatcgcctt
      781 gccagtccag agctcgagga tgtcttcgcg caggcgttcg ccggtcagca cgtccagcgc
      841 cgagaaggct tcgtccatca gcagcacctc gggctcgacg acgagcgcgc gcgcgatgcc
      901 cacgcgctgg cgcatgccgc ccgagagttc gcgcggcagc gcgccctcga agccggtcag
      961 gccgatcagc ttgatcgcgg cctcggcgcg ccgcgcgcgc tcggccggcg cgatgccgcg
     1021 cgcctccagc ccgagctcga cgttggcctg cacggtgagc cacgggaaca gcgcgaacga
     1081 ctggaacacc atgctgacgc cgcgcgccgg gccgtacagc ggctggtcgc gcaggcgcac
     1141 ctcgccgctg tcggcgccga tcagcccggc catgatgcgc agcatcgtcg acttgcccga
     1201 accgctctga ccgagcagcg cgacgatctc gccttcggcg agccggaagt cgacgccttc
     1261 gagcaccgag cgcgcactgc cgtcggccga cttgaacgac ttgccgacat tcttcagttc
     1321 gacgatggct tgggccatgg ttcggttcct tcgactcaaa agtgcatgcg gtcttcggcc
     1381 atgcggtaca gccgccgcca caggaagtgg ttcatgccca tcacgaacac gcacatcacg
     1441 ccgatgccca gcgcgatgcg cgggaagtcg ccgatcgccg tcatgtgggc gatgtagctg
     1501 cccaggccct gcgcctgcaa ggtagtgtcg ccccaggtca cgtactcggc gacgatgctg
     1561 gcattccacg agccgccgct ggcggtgatc gcgccggtca cgaagctcgg gaacaccgcc
     1621 ggcagcaggt agcggcgcca cttgagccag ccgcgcaggc cgaggttctt cgcggcgtag
     1681 cgcagctcgg tgggcaccgt cgaggcgccg gcgatcacgt tgaacagcag gtaccactgc
     1741 gtgcccagca ccatcagcgg cgagagccac aggttcgggt tcaggtgcca ggtgacgatg
     1801 aacacgaccg cgaccgggaa catcaggttc gccgggaacg ccgccagaaa ctgtgccgcc
     1861 gcctgcagcc gcccggccca gcgcgggttc atgccgatcc agacgccgat cggcacccac
     1921 acgagcgcgg cgagcgcgat cagcacgatc acgcgcagca tcgtgtagct gccgagcacg
     1981 aacacgtgca gcacctcggc aaagccgact tcggcacgga tgaaggcgac cagttgccag
     2041 accgcgagca gcacggcggc tgcgaccgcc gcgtcccaca cgcgcgacca ggctgggttc
     2101 ggcggcgctg tgcgggcgcg gatcgaggtg ccgtcgtggc tgcggcggaa cagcagcagc
     2161 gagcgttcga gcaggcgcac gaagcgcatc gcgagcgccg agagcgcatc ggtgcggcgc
     2221 atccaggtca gcagccacga ggtcggcgcg gtgtcattgc tgccttcctc gaagcggaat
     2281 ttgtcggccc aggcgagcag cgggcggaag aacagttggt cgtacagcac gatgcccacc
     2341 agcatgccgc cgatcgccca gccgatcgcg cgcaggtcac gctgctcgat cgccagtgcg
     2401 atgtacgagc cgacgccggg cagcttgatg ctctggttcg ccaccgagat cgcttccgag
     2461 gcgacgacga agaaccagcc gcccgacatc gacatcatca tgttccacag caaccccggc
     2521 atcgcgaacg gcagttcgag ccgccagaag cgctgccagc ccgaaagctg gaacacggcc
     2581 gccgcctcct gcagctcggc cggcacggtc ttgagcgact ggtacaggct gaacgccatg
     2641 ttccaggcct gcgaggtgaa gatcgcgaag atcgccgcac actcgacgcc gaacaggttg
     2701 ccggggaaga gggcgatgaa gcccgtcacc gtgatcgaca gaaagcccag gatcgggatc
     2761 gactgcagga tgtcgagcag cggcaccatc acgcgctcgg ccgcgcggta cttggccgca
     2821 accgccgcga acacgcaggc gaagacgagc gacgcggcga gcgcgaggaa catgcgcagc
     2881 gtggtgcgca gcaggtagta gggcaggtgg gtcgggtcca gcgtcagtgg cagcgcctga
     2941 cccagctggt atggccgcgc catctgcgag ccgccccagg ccagcagcgc gaacacgccc
     3001 agcacgagcg gcagcagcgc ccagtcccag cggttgccgg cggactcgac gacctgccgg
     3061 ggaaagaaac gcttgtcgat catcgg
//
LOCUS       DASRDG010000532         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_194615, whole genome shotgun sequence.
ACCESSION   DASRDG010000532 DASRDG010000000
VERSION     DASRDG010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194615"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2066
                     /locus_tag="VFA35_08925"
     CDS             <1..>2066
                     /locus_tag="VFA35_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56335.1"
                     /translation="PLVLVFEDIHWADDGLLEFITDHLGDRFTGPLLVVTTSRPELLD
                     RRPQWAAAARSTMLELQPLSDQETADVVTNLLDAAELPPELRSALLARAGGNPLFAEE
                     YVRMLLDRGLLRSEGSAPELTGTDLPLPESVQSIIAARLDALPSDEKLLLQDAAVVGR
                     AFWLGALAEVGERQRWSVEHMLHELERKQLLRRERDSIVLSEPQYAFSHALVRDVAYE
                     QIPRARRSRKHWRTAGWIESLSPERTEDRAEMLSHHYMRALRYLPQGDTVSIELLAAA
                     RSALCDAGDRSMSLNAFAEAAHFFSEALQLWPQELPGWADVAFRLGSAQVHAESGGDR
                     PLEQARDAFIAEGQPGKAAEAMVLIGELLWMRGDPDAFRHLDEAGALLRDTPPSYSKA
                     YVLSSLSRFHMIADENLRSIEIGQEALAMAEEMGLDELRAHALDSIGISRARIGDERG
                     IADLEQSIALAVAHNSLESVRGYANLGNAMVEYGDLARAFEVYQQGRDAAQRFGDVDR
                     ILWFEVERMYECYWRGRWDEAQRLADEIVAQVDAGSPSSSEHDARLVRARIRLGRGQE
                     PAALDDSTRALELGRHAGYPEMLVPSLALQARVLEAAGRGAEASQLADELLGIWPDRC
                     PTSYWVSDLAFTLRRVDRPDALARAARSAPAASRWLDAGLALAAGDAAGAAEKYAEIG
                     CLPDEA"
BASE COUNT          272 a          705 c          812 g          277 t
ORIGIN      
        1 gcccgctggt gctggtgttc gaggacatcc actgggccga cgacggcctg ctcgagttca
       61 tcaccgacca cctcggcgac cgcttcaccg ggccgctgct ggtggtgacc accagccggc
      121 cggagctgct cgaccggcgg ccgcagtggg ccgccgcggc ccgcagcacg atgctcgagc
      181 tgcagccgtt gtcggaccag gagacggccg acgtggtcac caacctgctg gacgcggcgg
      241 agctgccgcc ggagctgcgc tccgccctgc tggcgcgggc cggcggcaac ccgctattcg
      301 ccgaggagta cgtgcggatg ctgcttgacc gcgggctgct gcgcagcgag ggctcggcgc
      361 cggagctgac cggcaccgac ctgccgctgc cggaatcggt gcagtcgatc atcgccgccc
      421 gcctggacgc gctgccgtcg gacgagaagc tgctgttgca ggacgcggcg gtggtcggcc
      481 gcgcgttctg gctgggcgcg ctggccgagg tgggagagcg ccagcgctgg tcggtggagc
      541 acatgctgca cgaactggag cgaaagcagc tgctgcgccg ggagcgcgac tcgatcgtgc
      601 tgagcgagcc gcagtacgcc ttctcccacg cgctggtgcg cgacgtcgcc tacgagcaga
      661 tcccgcgcgc gcggcgctcg cgcaagcact ggcggacggc cggctggatc gagtcgctgt
      721 cgcccgagcg caccgaggac cgggccgaga tgctgtccca ccactacatg cgggcgctgc
      781 gctacctgcc gcagggcgac acggtcagca tcgagctgct ggccgcggcg cgcagtgcgc
      841 tctgcgatgc cggcgaccgg tcgatgtcgc tgaacgcgtt cgccgaggcg gcgcacttct
      901 tctccgaggc gctgcagctg tggccccagg agttgcccgg ctgggccgac gtggccttcc
      961 ggctgggcag cgcgcaggtg cacgccgaga gcggcggcga ccggccgctg gagcaggccc
     1021 gcgatgcctt catcgccgag ggccagcccg ggaaggcggc cgaggcgatg gtgctgatcg
     1081 gcgagctgct gtggatgcgc ggcgacccgg acgccttccg gcacctggac gaggcgggcg
     1141 cgcttctgcg cgatacgccg ccgtcctact cgaaggccta cgtgctgagc agcctgtcgc
     1201 gcttccacat gatcgccgac gagaacctgc ggtcgatcga gatcggccag gaggcgctgg
     1261 cgatggcgga ggagatgggc ctggacgagc tgcgggcgca cgcgctggac agcatcggca
     1321 tctcgcgcgc ccgcatcggc gacgagcgcg gtatcgccga cctcgagcag agcatcgcgc
     1381 tggcggtcgc gcacaactcg ctggagagcg tgcgcggcta tgccaacctg ggcaacgcga
     1441 tggtcgagta cggcgacctg gcgcgggcgt tcgaggtcta ccagcagggg cgcgacgccg
     1501 cgcagcggtt cggcgacgtc gaccgcatcc tctggttcga ggtggagcgg atgtacgagt
     1561 gctactggcg cgggcgctgg gatgaggcgc agcggctggc cgacgagatc gtggcgcagg
     1621 tggacgccgg ttcgccctcg tcctcggagc acgacgcgcg gctggtgcgg gcccggatca
     1681 ggctcggccg cggacaggag ccggcggcgc tggacgactc gacccgggcg ctggagcttg
     1741 ggcgccacgc cgggtacccg gagatgctgg tgccgtcgct ggcgctgcag gcgcgggtgc
     1801 tggaggcggc cgggcgcggg gcggaggcat cgcagctggc cgacgagctg ctggggatct
     1861 ggcccgaccg ctgcccgacc tcctactggg tgtccgacct ggccttcacg ctgcggcggg
     1921 tcgaccggcc ggacgcgctg gcaagggcgg cccgatcagc gccggcggcc agccgctggc
     1981 tggatgccgg cctggcgctg gccgccggcg acgcggccgg tgcggccgag aagtacgccg
     2041 agatcggctg cctgccggac gaggcc
//
LOCUS       DASRDG010000533         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_82164, whole genome shotgun sequence.
ACCESSION   DASRDG010000533 DASRDG010000000
VERSION     DASRDG010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82164"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFA35_08930"
     CDS             complement(<1..348)
                     /locus_tag="VFA35_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZT56336.1"
                     /translation="MPRLTLSTLNASALQIPGADRWYANPEDPALSVMTDFRERSSVT
                     VSETLSIDAALEHMKHTGVRCAFAVDEAQRLVVGLITAYDIMGEKPMRLMQATSTARS
                     HLLVKDLMTRLPDL"
     gene            437..>2224
                     /locus_tag="VFA35_08935"
     CDS             437..>2224
                     /locus_tag="VFA35_08935"
                     /inference="COORDINATES: protein motif:HMM:NF021615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZT56337.1"
                     /translation="MNGHWGPGPLRGLASATRQLRGCWWSMAGVSLLCNLLSTAAVVP
                     LVGLLLRWLVARSGQVAVADVEIAGFLFTTAPGIVALLLISALLLAVTAFEQACMLNI
                     ALSPQGRARVSVRAACAQAAARAGSILRFAARLVIRLLVLLAPFAAVAGLIYWALLRR
                     YDINYYLGDRPPQFWVALSLVALDLLALVALLMRYLARWLLGLPLVAVERCPPTQALA
                     ESSRRMKDHQRAAAGLLAAWAMAGLALSLLSLPLLQALGRAVAPAFGESLRGVLLFSV
                     LVALVGLAASFALSVTLKALFALITVHYYLTSATFGEPQPYARSRPAASAAETVVRRR
                     WPAVVVAIVGLVLAGTALGYSLLQGTLQDRAVLVFAHRGASAAAPENTLAAFRRAGAE
                     RADFVELDVQETADGVVVVAHDSDLMKVARSPLKIWNSTAAQLHAVDIGSFFAPAFSD
                     QRVPTLAEALDVCRGVSRVDIELKDYGHDQWLEQRVVELVEAAGMQDQVVTMSLSGRM
                     VARMKQLRPRWTSGQLIARAIGRPSALPVDFLAVESSMATRSFIRAAHEGGKPVYVWT
                     VNDAQRMIRLIGLGADGLITDHPALARDAV"
BASE COUNT          286 a          722 c          809 g          407 t
ORIGIN      
        1 caggtccggc agccgcgtca tgaggtcctt gaccagcagg tgcgagcgcg cggtggacgt
       61 ggcctgcatg aggcgcatcg gcttctcgcc catgatgtcg taggcggtga tcaacccgac
      121 cacgaggcgc tgcgcctcgt cgaccgcgaa tgcgcaacgg acgccagtgt gcttcatgtg
      181 ctcgagcgcg gcatcgatgc tgagcgtctc gctgacggtc accgagctgc gctcgcgaaa
      241 gtcggtcatc accgagagtg ccggatcttc cgggttcgcg taccagcggt cggcccccgg
      301 aatctgcagc gcggacgcat tgagggtgct gagcgttagc ctgggcattc gtacctcgtc
      361 ggggcgccgg tcattgtacc atctgcgaag tggctgcccg gcgggcctgg gtgggagaca
      421 gtagcggatg agcgccatga atgggcactg gggccctggg ccgctgcgcg ggctcgcaag
      481 cgcgacccgt caactgcgcg gctgctggtg gtcgatggct ggcgtgagcc tgctgtgcaa
      541 cctgctgtcc accgcggcgg tcgtgccgct cgtcggcctg ttgctgcgtt ggctcgtggc
      601 gcgcagcggc caggtggccg tcgcggacgt cgagattgcc ggcttcctgt tcacgaccgc
      661 tccggggatc gtggcgctgt tactgatcag cgcgctgttg ctcgccgtga cggcgttcga
      721 acaggcgtgc atgctgaaca tcgccttgtc gccacagggt cgagcccgcg tgagcgtgcg
      781 cgccgcctgc gcgcaggcgg cggcgcgcgc cggttcgatc ctgcgttttg ccgcccggct
      841 cgtgatccgc ctgctggtgc tgctggcgcc gtttgcggcg gtcgccggcc tcatttactg
      901 ggcgctgctg cgccgctatg acatcaacta ttacctgggc gatcggccgc cgcagttctg
      961 ggtcgcgctg tcgctcgtgg ccctcgacct gcttgccttg gtggcgctgc tcatgcgcta
     1021 tctggcgcga tggctgcttg gattgccgct cgtggccgtg gagcgctgcc cgcccacgca
     1081 ggccctggca gaaagttcgc ggcgcatgaa ggatcaccag cgtgcggcgg ccgggctgct
     1141 cgccgcgtgg gccatggccg gcctggcgct gtcgctgctg tcgctgccgc tgctgcaagc
     1201 gctcggacgg gctgtggcac cggcatttgg cgagtccctg cgcggcgtgc tgctgttttc
     1261 ggtgctggtc gcgctggtcg ggctggcggc gagctttgcg ctgtcggtga cgctcaaggc
     1321 gctcttcgcc ctcatcaccg tgcactatta cctgacaagc gccacgttcg gcgaaccgca
     1381 gccgtacgcg cggtcgcgac cggccgccag cgcggccgaa accgtggtgc gccggcgctg
     1441 gccggcagtc gtggtggcga tcgtcggact cgtcttggca ggcaccgcgc tcggatactc
     1501 gctgctgcag ggaacactgc aggatcgtgc ggtgctcgta ttcgcgcacc gcggtgcctc
     1561 ggcggcggcg ccggaaaaca cgctggccgc gttccggcgc gcgggcgcag agcgtgccga
     1621 tttcgtcgag ctcgacgtgc aggaaaccgc cgatggagtc gtggtcgtgg cgcacgacag
     1681 cgacctcatg aaggtcgcgc gctcgccgct gaagatctgg aactcgaccg cggcgcaact
     1741 acacgcggtc gacatcggca gcttcttcgc gccggcattc tctgaccagc gcgtgccgac
     1801 gctggccgag gcgctggacg tgtgccgcgg cgtttcgcgc gtcgacatcg agctcaagga
     1861 ttacgggcac gaccagtggc tcgagcagcg ggtcgtggaa ctggtcgagg ccgcgggtat
     1921 gcaagaccag gtcgtgacga tgtcattgag cgggcgcatg gtcgcgcgca tgaagcagct
     1981 gcggccacgc tggacctcgg ggcaactgat cgcgcgtgcc atcgggcggc cgagcgccct
     2041 gcccgtcgat ttcctggcgg tcgagtcgtc gatggcgacg cgcagtttca tccgcgccgc
     2101 gcacgaggga ggcaagccgg tttacgtctg gaccgtaaat gacgcgcagc gcatgatccg
     2161 gctcattggc ctcggcgccg atgggctgat caccgatcat ccggcgctgg cgcgcgatgc
     2221 agtg
//
LOCUS       DASRDG010000534         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_652853, whole genome shotgun sequence.
ACCESSION   DASRDG010000534 DASRDG010000000
VERSION     DASRDG010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652853"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VFA35_08940"
     CDS             complement(<1..1144)
                     /locus_tag="VFA35_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56338.1"
                     /translation="MVLTRKIGSFLRGKATRGQVFTAALLAGLLGFVPGFFLPGNLGG
                     GFLQAPGLILVLFFLVLVLNANFGVFGLVLVLAKLLSLVTLPLANALGNLLLNGPTQG
                     LFKPLINGPVTAWFGLHYYATTGGLVLGLLFGGAAGLLFVQALTRFRRHMAGLEENSD
                     RYQAASKKKSLRFLTWLLFGKGKGGKFSWQELAEGHKRALPVRAAGVAICALIGGGLW
                     LFHSVYLTPMLTSGVKSGLLAVNGATVDLDKAELDFTAGSLKFTNLALADAADLDKNA
                     FAAGALEAKIDTSALLRRRYVIDTLRASDASSGQPRKTRGELTKPPAPPPPPPPAGTK
                     TIDDYVKDIKLWQQRLEQARDWIAKLLGSGQKAPGAQTPEDVQKKRQ"
     gene            complement(1201..1665)
                     /locus_tag="VFA35_08945"
     CDS             complement(1201..1665)
                     /locus_tag="VFA35_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56339.1"
                     /translation="MRALVLLLLSLLAACEVAPWREQPPPWTAATVADVEDARVLLGD
                     GSELVLSGPRLEGEGGEALLLGEGEDGPCRVPLRDVCRLETRRTEVLRVIANAVIVTA
                     VVAGVVVLCACGGGHGGGLDGLNFGGGSAPEKATGCERVPAHRPPGHGRPPR"
     gene            complement(1758..>2257)
                     /locus_tag="VFA35_08950"
     CDS             complement(1758..>2257)
                     /locus_tag="VFA35_08950"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZT56340.1"
                     /translation="SARAGFDLPRQPVVAVTWFDAVAYCAWLAEATGLPCRLPTEAER
                     EKAARGGRAGARYPWGDGPRAHDALLAAPPAAGGGEANGYGLLHMADGVHEWCSDWYA
                     DDWYLRSPARDPLGPAAGARRVSRGGSWRHRVPVTPCAARSSLPPDRCYADYGFRVAI
                     GASGL"
BASE COUNT          308 a          824 c          827 g          298 t
ORIGIN      
        1 cctggcgctt cttctgcacg tcctcgggcg tctgcgcgcc cggcgccttc tggccgctgc
       61 ccaggagttt cgcgatccag tcgcgcgcct gctcgaggcg ctgctgccac agtttgatgt
      121 ccttgacgta gtcgtcgatc gtcttggtgc ccgccggcgg cggtggcggc ggcggcgctg
      181 gcggcttcgt cagctcgccg cgcgtcttgc gcggctggcc gctgctcgcg tcgctggcgc
      241 gcagcgtgtc gatcacgtag cgccgccgca gcagcgcaga ggtgtcgatc ttcgcctcca
      301 gcgcgccggc ggcgaacgcg ttcttgtcga ggtcggcggc gtcggcgagc gccaggttgg
      361 tgaacttgag cgagccggcg gtgaagtcga gctccgcctt gtcgaggtcg acggtcgcgc
      421 cgttgaccgc gagcaggccg ctcttcacgc cgctggtcag catcggcgtg aggtagacgc
      481 tgtggaacag ccacaggccg ccgccgatca gggcacagat tgcgacgccc gcggcgcgca
      541 ccggcagcgc gcgcttgtgg ccctcggcga gctcctgcca gctgaacttg ccgcccttgc
      601 ccttgccgaa cagcagccag gtgaggaagc gcagcgactt cttcttgctc gctgcctgat
      661 agcggtcgct gttctcctcg aggccggcca tgtgccggcg gaagcgcgtg agcgcctgca
      721 cgaacaggag cccggcggcg ccgccgaaca gcaggccgag caccagcccg ccggtggtcg
      781 cgtagtagtg caggccgaac cacgcggtga ccgggccgtt gatcagcggc ttgaacaggc
      841 cctgggtcgg gccgttgagc agcaggttgc cgagcgcgtt cgcgagcggc agcgtcacca
      901 ggctcaggag cttggcgagc accagcacga gcccgaacac gccgaagttg gcgttgagca
      961 ccagcacgag gaagaacaac acgaggatca gccccggcgc ctgcaggaag ccgccgccga
     1021 ggttgccggg caagaagaag cccggaacga agccgagcag gccggcgagc agcgccgcgg
     1081 tgaagacctg gccgcgcgtc gccttgccgc gcaggaagct gccgatcttg cgagtcagga
     1141 ccatgcgcgc agcgtagcgg cgcgatgcgc gcgagacgac ggcgcggtgc gcagcgccgt
     1201 tcaacgcggc ggtcggccgt gccccggcgg acgatgcgcc ggaacccgct cgcaccccgt
     1261 tgccttctcg ggagcgctcc cgcccccgaa gttcaggccg tcgagtccgc cgccgtgacc
     1321 gcctccacag gcgcacagga cgacgacgcc ggcgaccacg gcggtgacga tcacggcgtt
     1381 ggcgatcacg cgcagcacct cggtgcgcct cgtctcgagg cggcagacgt cgcgcaacgg
     1441 gacgcggcac gggccgtcct caccctcgcc gagcagcagt gcctctccgc cttcgccttc
     1501 gagccgcggc ccggacagca cgagttcgct gccgtcgccg agcagcacgc gcgcatcctc
     1561 gacgtcggcg accgtggccg ccgtccacgg cggcggctgc tcgcgccacg gcgccacttc
     1621 gcacgcggcg aggagactca gcaacagcag gacgagcgcg cgcatcatgc cccttcgccg
     1681 cagcgccagc cgcggcgccg ccgttgcgcg tgctgcgcgt gagccttgtc atcgggaagt
     1741 cggtcggcgg tcgcggctca gagaccgctg gcgccgatgg cgacgcggaa gccgtagtcg
     1801 gcgtagcagc ggtcgggcgg caggctgctg cgcgcggcgc acggcgtgac gggcacgcgg
     1861 tggcgccacg agccgccgcg cgagacgcgg cgcgcgccgg ccgcggggcc gagcgggtcg
     1921 cgcgcgggtg aacgcaggta ccagtcgtcc gcgtaccagt cgctgcacca ttcgtgcacg
     1981 ccgtcggcca tgtgcaacag gccgtagccg ttcgcttcgc cgccgccggc cgccggcggc
     2041 gccgcaagca gcgcatcgtg cgcgcgcggt ccgtcgcccc acggatagcg cgcgccggcg
     2101 cggccgccgc gcgcggcctt ctcgcgttcg gcttcggtcg gcagccggca cggcaggccc
     2161 gttgcttccg cgagccacgc gcagtacgcg accgcgtcga accaggtcac cgcgaccacg
     2221 ggttgccgcg gcaggtcgaa gcccgcgcgc gccgaca
//
LOCUS       DASRDG010000535         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_885194, whole genome shotgun sequence.
ACCESSION   DASRDG010000535 DASRDG010000000
VERSION     DASRDG010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885194"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFA35_08955"
     CDS             complement(<1..421)
                     /locus_tag="VFA35_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HZT56341.1"
                     /translation="MTGAASAREAGRWAVAFAGAVALAVAMGIGRFAFTPLLPMMLHD
                     GVIDLHAASWLASANYLGYLAGALLCTFQPWIWRRAPRLPAIDGVWLLRAGLVATGLL
                     TLGMALPLPAAWSLLRFAAGVASAVVFVYTSGWCLTQL"
     gene            complement(418..963)
                     /locus_tag="VFA35_08960"
     CDS             complement(418..963)
                     /locus_tag="VFA35_08960"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HZT56342.1"
                     /translation="MKHALPAILLALTAIGAHAQTQAPGLWEHSFTMKSPGGEMERAQ
                     AEMHKQLAAMPPAERKKIEDMMASRGVKTGAQGTTAKFCLTKEQAAKPAEPHMSGDCA
                     QSDVKRSGNTVSYKFACTKPHPVSGEGQVSYLGDKAYTGRSSLTTQAKGVPQQMTMEM
                     SGKWLAADCGDVKPLGASTAR"
     gene            complement(998..>2015)
                     /locus_tag="VFA35_08965"
     CDS             complement(998..>2015)
                     /locus_tag="VFA35_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZT56343.1"
                     /translation="FGNVPVIAGIFFGLKPAVAALVVHAAHRIGSRALKNRWLWAIAA
                     AAFVAIFAFDLPFPLIVLAAGLIGHFGGRHAPRLFALGGGHGAAQASFGPALIDDATP
                     TPPHARFTRGRLVRVLLVGLGLWAAAMGALVAAVGIDGTLTQMGWFFTKAALLTFGGA
                     YAVLPYVYQGAVEHQHWLTGAQMIDGLALGETTPGPLIMVVAFVGFVGGWVRQVLGAD
                     ALFLGAALAASVVTFFTFLPSFVFILAGGPVVESTHGNLKFTAPLTAITAAVVGVILN
                     LALFFGYHVLWPQGWGGPFSVASALITAGAAIALFRFRVGVIPLLLACAALGLALTLA
                     GAVN"
BASE COUNT          312 a          736 c          718 g          249 t
ORIGIN      
        1 ccagctgcgt gaggcaccag cccgaggtgt agacgaacac gaccgcgctg gcgacgccgg
       61 ccgcgaagcg cagcagcgac cacgccgccg gcagcggcag cgccatgccg agcgtcagca
      121 gcccggtcgc gaccagcccg gcacgcagca gccacacgcc gtcgatcgcc ggcaggcgcg
      181 gcgcgcgccg ccagatccac ggctggaagg tgcacagcag cgcgccggcc aggtagccga
      241 ggtagttggc gctggccagc cagctcgcgg cgtgcaggtc gatcacgccg tcgtgcagca
      301 tcatcggcag cagcggcgtg aacgcgaagc ggccgatgcc catcgcgacc gcaagcgcca
      361 ccgcgccggc gaacgcgacg gcccagcggc ccgcttcgcg agccgaagcg gcgcccgtca
      421 ccgggccgtc gaggcgccga gcggcttcac gtcgccgcag tcggcggcca gccacttgcc
      481 ggacatctcc atcgtcatct gctgcggcac gcccttcgcc tgcgtcgtca ggctcgacct
      541 gccggtgtag gccttgtcgc cgaggtagct gacctgccct tcgccgctga cggggtgcgg
      601 cttcgtgcac gcgaacttgt agctgaccgt gttgccgctg cgcttcacgt ccgactgcgc
      661 gcagtcgccg ctcatgtgcg gctcggccgg cttcgcggcc tgctccttgg tgaggcagaa
      721 cttcgcggtc gtgccttgcg cgcccgtctt gacgccacgg ctggccatca tgtcttcgat
      781 cttcttgcgc tcggccggcg gcatcgcggc gagttgcttg tgcatctcgg cctgtgctcg
      841 ctccatctcg ccgcccggcg acttcatcgt gaagctgtgt tcccacaggc ccggcgcctg
      901 ggtctgcgcg tgcgcgccga tcgcggtcag cgccagcaag atggcgggca gggcgtgctt
      961 catcgcggtt cctcgggtgg ttgcgggggc ggacgtgtca gtttacggcg cctgccaggg
     1021 tcaacgcgag gccgagcgcc gcgcacgcga gcagcagcgg gatcacgccg acccggaagc
     1081 ggaacagcgc gatcgccgcc cccgccgtga tcaatgccga ggcgacgctg aacggcccgc
     1141 cccacccctg cggccacagc acgtggtacc cgaagaacag cgcgaggttc aggatcacgc
     1201 cgacgacggc ggcggtgatc gcggtcagcg gcgcggtgaa tttcaggttg ccgtgggtcg
     1261 actcgaccac cggcccgccc gcgaggatga acacgaacga cggcaggaag gtgaagaagg
     1321 tgacgacgct cgccgccagc gccgcgccga gaaacagcgc gtcggcgccg agcacctgcc
     1381 ggacccagcc gccgacgaag ccgacgaacg cgaccaccat gatcagcggc cccggcgtgg
     1441 tttcgccgag cgcgaggccg tcgatcatct gcgcgccggt cagccagtgc tggtgctcga
     1501 ccgcgccctg gtagacgtag ggcagcacgg cgtaggcgcc gccgaaggtc agcagcgcgg
     1561 ccttggtgaa gaaccagccc atctgcgtca aggtgccgtc gatgccgacc gcggcgacca
     1621 gcgcgcccat cgccgcggcc cagagcccga ggccgacgag cagcacgcgc accagccggc
     1681 cgcgcgtgaa ccgcgcgtgc ggcggcgtcg gcgtggcgtc gtcgatcaac gcggggccga
     1741 acgacgcttg cgcggcgccg tggccgccgc cgagcgcgaa caggcgcggc gcgtggcggc
     1801 caccgaagtg cccgatcaga cccgccgcga gcacgatgag cgggaacggc aggtcgaacg
     1861 cgaagatcgc gacgaaggcc gcggcggcga tcgcccacaa ccagcggttc ttcagcgcgc
     1921 gcgagccgat gcggtgcgcc gcgtgcacga ccagcgccgc gaccgccggc ttcagcccga
     1981 agaagatgcc ggcgatcacc ggcacgttgc cgaac
//
LOCUS       DASRDG010000536         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_313041, whole genome shotgun sequence.
ACCESSION   DASRDG010000536 DASRDG010000000
VERSION     DASRDG010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313041"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFA35_08970"
     CDS             complement(<1..792)
                     /locus_tag="VFA35_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HZT56344.1"
                     /translation="MRSPLQDLSLSAATAGFVAVLVGFTSSVAIVFSAATALGASPAQ
                     ISSWMWALGLGMGLCTALPSLWLRQPVMVAWSTPGAAVLATAGAAGGFGMAEATGAFI
                     ACAALIALFGATGWFERLLVRIPMPIASALLAGVLTRFGLDAFVALQSALGLVLLMLI
                     TYLVGRRFWARYAVPGVLLAGIAYAAVNGRLHLGGVHWGLTLPVFTVPEFTWRAMVSL
                     ALPLFVVTMASQNLPGVAAIRAAGYAMPVSKIVTLTGLATLVLAPF"
     gene            876..1097
                     /locus_tag="VFA35_08975"
     CDS             876..1097
                     /locus_tag="VFA35_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56345.1"
                     /translation="MRTTLDLSPTALEAARQLAAHRAQSLGTIVSELILKGLRAETPV
                     ARKSGFPVFAGEAGKTITLDDVKAAEDES"
     gene            1094..1603
                     /locus_tag="VFA35_08980"
     CDS             1094..1603
                     /locus_tag="VFA35_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HZT56346.1"
                     /translation="MTRSTVWLLDANLLIALTHSAHIHHAEAHTWFASHPKRRWATCA
                     LTQLAFVRLTSNPKVVGSEIAPAEATQALATMTGQATHDYWADAPAPLTLATLRSAAL
                     VGHRQVTDAYLLGLAALRQQRLATLDRGLVSFAAGTGLAANVELVSAASMAQQPVTRY
                     AVGRVRSRP"
     gene            complement(1587..>2142)
                     /locus_tag="VFA35_08985"
     CDS             complement(1587..>2142)
                     /locus_tag="VFA35_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HZT56347.1"
                     /translation="TWKQGRKLMGVRMREDAIDLKSFLEAVFVSPPTRVSGTAVFLVS
                     DAGLTPNALLHNLKHNKVLHETNLFVTVKQHEVPWIGFDKRCEMEALGHDCWQVTLNF
                     GFKNEPDVPEALALLERRGCVIDEMDTSYFLSRDIVIPTMGTGMADWREKLFASMHRN
                     AAAAADFLSLPTNRVVELGAKVEI"
BASE COUNT          367 a          782 c          713 g          280 t
ORIGIN      
        1 gaagggcgcg agcaccagcg tcgcgaggcc ggtcagcgtg acgatcttcg agaccggcat
       61 cgcgtagccc gccgcgcgga tcgcggcgac gccaggcagg ttctgcgacg ccatcgtcac
      121 gacgaacagc ggcagcgcca ggctcaccat cgcgcgccag gtgaattcgg gcaccgtgaa
      181 caccggcagc gtcaggcccc agtgcacgcc gccgaggtgc aaccggccgt tgaccgccgc
      241 atacgcgatg ccggccagca gcacgcccgg caccgcgtag cgcgcccaga agcgccggcc
      301 gacgaggtag gtgatcagca tcagcagcac gagcccgagc gcggactgca gcgcgacgaa
      361 cgcatcgagc ccgaagcgcg tgagcacgcc ggcgagcagc gcactcgcga tcggcatcgg
      421 gatgcggacg agcaacctct cgaaccaacc ggtggcgccg aacagcgcga tcagcgcggc
      481 gcaggcgatg aacgcgccgg tcgcctcggc catgccgaag ccgccggccg cgcccgcggt
      541 cgcgagcacc gcggcgccgg gtgtgctcca cgcgaccatc accggctggc gcaaccacag
      601 cgagggcagc gcggtgcaca gccccatgcc caggccgagc gcccacatcc acgagctgat
      661 ctgcgccggg ctggcgccga gcgcggtcgc agccgagaac acgatcgcga ccgagctggt
      721 gaaccccacc agcaccgcga cgaagccggc ggtcgcggcg gaaagggaga ggtcttgcag
      781 gggcgagcgc atcggacaca ggatagcttc gggccgtccg ccgacagcgc cgatgctatg
      841 atgctatcgc atcaactcat cagttgaaca ccgccatgcg caccaccctc gacctcagcc
      901 ccaccgcgct cgaagcggcc cgccaacttg ccgcgcaccg cgcgcagtcg ctggggacga
      961 tcgtttccga actgatcctg aagggcttgc gcgccgagac gcccgtcgcg cgcaagagcg
     1021 gcttcccggt gttcgcgggc gaggcgggca agaccatcac gctcgacgac gtgaaggcgg
     1081 ccgaggacga gtcttgactc gcagcacggt ctggctgctc gatgccaacc tgctgatcgc
     1141 gctgacccac tcggcgcaca tccatcacgc cgaagcacac acctggttcg catcgcatcc
     1201 gaagcgccgc tgggccacct gcgcgctgac gcagctcgcc ttcgtgcggc tgacgagcaa
     1261 ccccaaggtt gtcggcagcg agatcgcgcc ggccgaagcg acgcaggcgc tggccacgat
     1321 gacggggcag gccacgcacg actactgggc cgacgcgccc gcgccgctga cactcgccac
     1381 gctccgcagc gcggcgctgg tcggccatcg gcaggtgacc gatgcgtacc tcctcggcct
     1441 ggcggcgttg cgccagcaac gcctcgccac gctcgaccgc ggcctcgtgt cgttcgccgc
     1501 gggcaccggc ctggccgcga acgtggagct cgtcagcgca gcatcgatgg cgcagcaacc
     1561 ggtgacgcgc tacgccgtcg ggcgcgtcag atctcgacct tagcgcccag ctccacgacc
     1621 cggttcgtcg gcaggctcag gaagtcggcc gccgccgcgg cgttgcggtg catgctcgcg
     1681 aacagcttct cgcgccagtc ggccatgccg gtgcccatcg tcgggatcac gatgtcgcgc
     1741 gagagaaagt acgaggtgtc catctcgtcg atcacgcagc cgcggcgttc gagcagcgcg
     1801 agcgcctcgg gcacgtcggg ctcgttcttg aagccgaagt tcagcgtcac ctgccagcag
     1861 tcgtgaccca gggcttccat ctcgcagcgc ttgtcgaacc cgatccacgg cacctcatgc
     1921 tgcttgacgg tgacgaacag gttggtctcg tgcagcacct tgttgtgctt caggttgtgc
     1981 agcagcgcgt tgggcgtcag ccccgcgtcg ctgaccagaa acaccgcggt gcccgacacg
     2041 cgcgtcggcg ggctcacgaa cacggcctcg agaaagctct tcaggtcgat cgcgtcttcg
     2101 cgcatgcgca cgcccatcag cttgcggccc tgcttccagg tc
//
LOCUS       DASRDG010000537         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1114715, whole genome shotgun sequence.
ACCESSION   DASRDG010000537 DASRDG010000000
VERSION     DASRDG010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114715"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="VFA35_08990"
     CDS             complement(<1..112)
                     /locus_tag="VFA35_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZT56348.1"
                     /translation="MKVAALQMVSTTNLEANLAAARRLIAQAARDGAELVA"
     gene            complement(126..4232)
                     /locus_tag="VFA35_08995"
     CDS             complement(126..4232)
                     /locus_tag="VFA35_08995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HZT56349.1"
                     /translation="MPRRADPGSRWAPLRWVARALLGLVFTAWSLLLIAWLTLHWGIL
                     PHIQQWREPIEQRASKALGVPVRIGAIEVRSSGWVPSFELREVVLLDAGNAPALRLPR
                     VFAAISPRSLLSLELRFEQLLIDGAQLEIRRDASGRIFVAGLDFSGADVANGAAGSAA
                     ADWFFGQGEFVIRGGALRWTDEQRKAPPLALTDVQLIVRNGLRLHALRLDATPPAEWG
                     ERFTASGRFTQPLLARRGDWRRWNGTAYANLPRADVRELRRYVNLPFELSEGVGSLRG
                     WFDLHAGTPTAATVDMALRAVSLRLAANVDPLVVEEVEGRLIAQRSADGVNLALQRFT
                     FETGDNIRWPQGDMKLAWRQRDGQPASGGEFSAQRLDLALMAQVASRVPLGAALRKLL
                     ADVDPRGTITDLAAGWDGPLDAPVRYRAKGALAGLSLAARASSEPRGVGRPGLRGAAM
                     QLEATEAGGSARIAIHGGALVLPGVFDDPLLALDQLSAQLLWKIEPSGAAGVTGVAGA
                     AKKISVQVKEARFSNADASGELSASWSTGPGAGVARGGRLPGRLELDGKLVNGVAKRT
                     FRYLPLGLSKGTRDYLEYALQGGKLTNASFHVRGDLWDFPFFDAKTPKDGEFRIGAKI
                     EDASFAFVPSLPASGGTPAVASAWPVLTKVGVDLALDRGTLELRSGRTQLGGIDFPKV
                     QATIRNLDADPVLTLDAAGRGPLAEMLRIVNTTPVGGWIDKALETSTATGAADLQLNL
                     AIPLMKVAATSAKGSVVLAGNDLRIAPETPLLAGAKGRVDFTHKGFAVVGASAKLYGG
                     DASFDGGSQPDGSVRFNGQGSVSADGLRRATEIGPIARLGGVLTGQASYRASLGFVHG
                     QPELNVSSNLVGLGINLPAPLAKAAEAPLALRLTTSVEAAAAGAAAPLRDTLRVELGN
                     LVHASYQRELSGAAPRVLRGGIGVQEPAPQPASGVAASVSLERLATDEWQAVADKLFG
                     AGDARDGRDGRDSGAGSPYVPDAVALHVQQLIAGPRRLTHLSAGLSQDAGVWRANVDA
                     DQLAGYVEYRVPTRRGAAAGGRVYARLARLSLPKGDDEQVEALLDQQPETMPALDIVV
                     DNFELRGKQLGRLEIEAVNRSGGPGRDWQLSKFNLTTPEAQLTATGHWAAVAPAAGRG
                     APVPRRAVMDFKLQLADSGALLDRLGTRGAVRGGKGQLSGQIGWIGSPFALDYPTLAG
                     QVNVAIDSGQFLKVEPGAARLLGVLSLQSLPRRLSLDFRDLFQEGFAFDSLTGDLKIA
                     QGVAVTNNLRMRGVQALVLMEGSADIARETQELRVVVVPEINAGTAALAYAVINPAIG
                     LGAFLAQAILKKPLTEAGTREFQISGPWADPKVERVEHRFGDDAASKK"
BASE COUNT          598 a         1668 c         1651 g          618 t
ORIGIN      
        1 gcgcgaccag ttcggcgccg tcgcgcgcgg cttgcgcgat caggcggcgc gcggccgcca
       61 ggttggcctc gaggttcgtc gtcgagacca tctgcaatgc ggcgaccttc atgcgcaacc
      121 ccttctcact tcttgctcgc cgcatcatcg ccgaaacggt gctcgacgcg ctcgaccttc
      181 gggtcggccc acgggccgct gatctggaac tcgcgcgtgc cggcctcggt cagcggcttc
      241 ttcaggatcg cctgcgcgag aaacgcgccc aggccgatcg ccgggttgat caccgcatac
      301 gcgagcgcgg cggtgccggc gttgatctcg gggacgacga cgacgcgcag ctcctgcgtc
      361 tcgcgcgcga tgtcggcgct gccttccatc agcaccagcg cctgcacgcc gcgcatgcgc
      421 aggttgttgg tcaccgccac gccttgcgcg atcttcaggt cgccggtgag gctgtcgaac
      481 gcgaagccct cctggaacag gtcgcggaag tcgagcgaga gccggcgcgg cagcgactgc
      541 aggctcagca cgccgagcag ccgcgcggcg cccggctcga ccttcaggaa ctggccggag
      601 tcgatcgcga cgttgacttg gcccgccagc gtcgggtaat cgagcgcgaa cggcgagccg
      661 atccagccga tctgccccga gagctggccc ttgccgccgc gcaccgcgcc gcgcgtgccg
      721 agccggtcga gcagcgcgcc gctgtcggcg agctgcagct tgaagtccat caccgcgcgc
      781 cgcggcaccg gggcgccgcg ccccgcggcc ggcgcgaccg cggcccagtg gccggtcgcg
      841 gtcagctggg cttctggcgt ggtgagattg aacttcgaca actgccagtc gcggcccggc
      901 ccgcccgaac ggttgacggc ctcgatctcg agccgtccga gctgcttgcc gcgcagctcg
      961 aagttgtcga cgacgatgtc gagcgcaggc atcgtctcgg gctgctggtc gagcaaggcc
     1021 tcgacctgct cgtcgtcacc cttcggcagc gacagccgtg cgagccgcgc atagacgcgc
     1081 ccgccggcgg cggccccgcg ccgcgtcggc acgcggtact cgacatagcc ggcgagttgg
     1141 tcggcgtcga cgttggcgcg ccagacaccc gcgtcctgcg acagcccggc cgacagatgc
     1201 gtcagccggc gcgggcccgc gatcagttgc tgcacgtgca gcgcgaccgc atcgggcacg
     1261 tacggcgagc cggcgcctga atcacggcca tcgcggccgt cgcgcgcgtc gccggcgccg
     1321 aacagcttgt cggcgaccgc ctgccactca tcggtggcga gccgctccag gctgacgctc
     1381 gcggcgacgc cgctggccgg ctgcggcgcg ggctcttgca cgccgatgcc gccgcgcagc
     1441 acgcgcggcg ccgcgccgct cagctcgcgc tggtagctcg cgtggaccag gttgccgagc
     1501 tcgacgcgca gcgtgtcgcg caacggcgcc gcggcgccgg cggccgcggc ctcgacgctg
     1561 gtcgtgaggc gcagcgcgag cggcgcctcg gccgccttcg cgagcggcgc cggcaggttg
     1621 atgccgaggc cgacgaggtt gctgctgacg ttgagttccg gttgaccgtg cacgaagccc
     1681 aggctcgcgc ggtagctcgc ctggcccgtc agcacgccgc cgaggcgcgc gatcgggccg
     1741 atctcggtgg cgcggcgcag gccatcggcg ctgacgctgc cctggccgtt gaagcgcacg
     1801 ctgccgtcgg gctgcgagcc gccgtcgaag ctcgcgtcac cgccgtacag cttcgcgctc
     1861 gcgccgacga ccgcgaagcc cttgtgcgtg aagtcgacgc ggcccttcgc gccggccagc
     1921 agcggcgtct cgggggcgat gcgcaggtcg ttgccggcga gcacgacgct gcccttcgcg
     1981 ctcgtcgccg cgaccttcat caacgggatc gcgaggttca gctgcaggtc ggcagcgccg
     2041 gtggcggtgc tcgtctcgag cgccttgtcg atccagccgc ccaccggcgt ggtgttgacg
     2101 atgcgcagca tctcggcgag cggcccgcgg ccggcggcgt cgagcgtcag caccgggtcg
     2161 gcgtcgaggt tgcggatcgt cgcctgcact ttcggaaagt cgatcccgcc gagctgggtg
     2221 cgcccgcttc gcaactcgag cgtgccgcgg tcgagcgcga ggtcgacgcc gaccttggtc
     2281 agcaccggcc aggccgaggc caccgccggc gtgccgccgc tggccggcag gctgggcacg
     2341 aacgcgaagc tcgcgtcctc gatcttcgcg ccgatgcgga actcgccgtc cttcggcgtc
     2401 ttcgcgtcga agaacggaaa gtcccagagg tcgccgcgca cgtggaagct cgcgttcgtc
     2461 agcttgccgc cttgcagcgc gtactcgagg tagtcgcgcg tgcctttcga caagccgagc
     2521 ggcaggtagc ggaaggtgcg cttggccacg ccattcacga gcttgccgtc gagctcgagc
     2581 cgcccgggca ggcgcccgcc gcgggccacg ccggcgccgg ggccggtgct ccacgacgcg
     2641 ctgagttcgc cgctcgcgtc ggcgttcgag aagcgcgcct ccttgacctg cacgctgatc
     2701 ttcttcgctg ccccggcaac gcccgtgacg ccggcggcac cgctcggctc gatcttccac
     2761 agcagctgcg cgctgagctg gtcgagcgcc agcagcgggt cgtcgaacac gccgggcagg
     2821 acgagcgcgc cgccgtggat cgcgatgcgc gcgctgccgc cggcctcggt cgcctcgagc
     2881 tgcatggccg cgccgcgcag ccccgggcgg ccgacaccgc gcggctcgct cgacgcgcgc
     2941 gccgccagcg acaggccggc cagcgcgccc ttcgcgcggt agcgcacggg cgcgtcgagc
     3001 gggccgtccc agccggccgc gaggtcggtg atcgtgccgc gcgggtcgac gtcggcgagc
     3061 agcttgcgca gcgccgcgcc gagcgggacg cggctcgcca cctgtgccat cagcgccagg
     3121 tcgagccgct gtgcgctgaa ctcgccgccg ctcgccggct gcccgtcgcg ctgccgccac
     3181 gcgagcttca tgtcgccctg cggccagcgg atgttgtcgc cggtctcgaa ggtgaagcgc
     3241 tgcagcgcga ggttcacgcc atcggcgctg cgctgcgcga tcagccggcc ttcgacctcc
     3301 tcgacgacga gcgggtcgac gtttgcggcc aggcgcaggc tcaccgcgcg cagcgccatg
     3361 tcgacggtcg cggcggtcgg cgtgccggcg tgaaggtcga accagccgcg cagcgagccc
     3421 acgccttcgc tgagctcgaa cggcaggttg acgtagcggc gcagctcgcg cacgtcggcg
     3481 cgcggcaggt tcgcgtaggc ggtgccgttc cagcgccgcc agtcgccgcg gcgcgcgagc
     3541 agcggctggg tgaagcgccc gctcgcagtg aagcgctcgc cccactcggc gggcggggtc
     3601 gcgtcgaggc ggagcgcatg caggcgcagg ccgttgcgca cgatgagctg gacatcggtc
     3661 agcgcgagcg gcggcgcctt gcgttgctcg tcggtccagc gcagtgcgcc gccgcggatc
     3721 acgaactcgc cctggccgaa gaaccagtcg gcggccgcgc tgccggcggc gccgttcgcg
     3781 acgtcggcgc cgctgaagtc gagcccggcg acgaagatgc ggccgctcgc gtcgcgccgg
     3841 atctcgagtt gcgcgccgtc gatcagcagc tgctcgaagc gcagctcgag cgacagcagc
     3901 gagcgcggcg agatcgccgc gaacacgcgc ggcaggcgca gcgcgggcgc gttgccggcg
     3961 tcgagcagga ccacttcgcg cagctcgaaa ctcggcaccc agccgctcga tcgcacctcg
     4021 atcgcaccga tgcgcaccgg cacgccgagc gccttgctgg cgcgttgttc gatcggctca
     4081 cgccattgct ggatgtgggg cagaatcccc caatggagtg tcagccacgc gatcagcaat
     4141 aagctccacg cggtgaaaac cagcccgagc agagcgcgtg ccacccagcg cagcggcgcc
     4201 cagcgcgagc ccgggtcggc gcggcgcggc acgggggcgg ggctcgcgtc ggagtgagtc
     4261 gtggcaggcg ggatcaagga catgaaaaca agagtcagga gccagccgcg ggtcgggcca
     4321 gtcaaggctc agaaggatgg gcagcgggcg tttgcgcagg ctgccggcga gaccgttgtt
     4381 ggcgcccgcc gctgacgggg caatctagca cagacccccg cgcactccat gggttccggc
     4441 gcttccttta ccacccccga tattggcgcc gccgcaccgg ccgaggccgc gcacagccgc
     4501 ttcgtgcagc gcatccgccg gcgctacccg acgga
//
LOCUS       DASRDG010000538         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1007559, whole genome shotgun sequence.
ACCESSION   DASRDG010000538 DASRDG010000000
VERSION     DASRDG010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007559"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFA35_09000"
     CDS             complement(<1..809)
                     /locus_tag="VFA35_09000"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZT56350.1"
                     /translation="MKRAFHARPIRRAFITAFGSVLAAAQGTSAQETTDSRGVVRIEV
                     TGSNIPRTEGETALPVQIITREEIAHSGSTTVAEVMARVSANIFGYNDQLSLIDQLGR
                     PGMSSVNLRGIGAGSTLTLLNGRRVANYAFEGEAVDVNSIPLAAIDRIEILKDGASAI
                     YGTDAIAGVVNFILRKDYQGFGADAFGAWTEHGGGDQYQANLSAGYGDLARDRFNAFV
                     TATYQKDQALRAADRRFSRTGYIPAEGIELLTLPSFPANIWSEDAGRFFSPA"
     gene            complement(847..1590)
                     /locus_tag="VFA35_09005"
     CDS             complement(847..1590)
                     /locus_tag="VFA35_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZT56351.1"
                     /translation="MTDCVIAEDEPILRAELKDSLTRLWPGLNIVAEAEDGIDALDAL
                     GRCKPQVMFLDIEMPGLSGLEVARAASGRCHVVFVTAYNQHAVSAFEQGAVDYVMKPF
                     SAARLATAIARVRERLDAAPARFDAVLDTLREQAPKRGYLRWINASQGQNVRLVTTEE
                     VCYFKADSKYTLVVTAQQESLIRRAIKELVQELDPAQFWQIHRGTVININAIAGVHRD
                     IGGHMRVKLKDRKETLPVSDPYVHLFRSM"
     gene            complement(1587..>2382)
                     /locus_tag="VFA35_09010"
     CDS             complement(1587..>2382)
                     /locus_tag="VFA35_09010"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZT56352.1"
                     /translation="FLPPGSTHVFNPFHPVLHRLDLMLIGMLVGGLATFVHVNRRTAM
                     TARRRQHEAEQARARARRKTLESQLQALQARVEPMFLFGTLERIRQLYRSDAAAAGAM
                     LEDLIVYLRAALPHLRESSSTVEKEAALAQAWLDIVGRSAQGRRVEFDIAGAAREARL
                     PALVMLPLVQQAVGGVESGALRLRVSVAIVAGRLCIEVSTSGGGFAAGIPGQPLLEQI
                     DERLRALYGAGARFEAGPDGGAALASRAQIELPLELDDLEPRGESR"
BASE COUNT          372 a          799 c          814 g          397 t
ORIGIN      
        1 gcggggctga aaaatcgacc cgcatcctct gaccatatgt tcgcaggaaa cgagggaagg
       61 gtcagcagtt cgatgccctc ggcggggatg tagcccgtgc gcgagaagcg cctgtcggcc
      121 gcgcgcagcg cctggtcttt ctggtaggtg gcggtcacga aggcattgaa ccggtcgcgc
      181 gcaagatcgc catagccagc ggaaagattg gcctggtact ggtcgccgcc gccgtgctcc
      241 gtccacgcgc cgaatgcgtc cgcgccgaag ccctgataat ccttgcgcag gatgaagttc
      301 acgacgccgg caatcgcgtc ggtgccgtag atcgccgacg cgccatcctt gaggatctcg
      361 atgcgatcga tggctgcgag cgggatcgag ttcacgtcga cggcttcacc ttcgaacgcg
      421 tagttggcga cgcggcgtcc attgagcaga gtgagcgtcg aaccggcgcc gatgccgcgc
      481 aggttcacac tggacatgcc gggccgcccc agctgatcta tcagcgaaag ctggtcgttg
      541 tagccgaaaa tattcgccga gacccgtgcc atcacttcgg ccaccgtcgt cgagccgctg
      601 tgcgcaatct cctcgcgcgt gatgatctgc accggcagcg ccgtttcgcc ctcggtgcga
      661 ggaatgttgc tgcccgtgac ttcgatccgg acgacgcctc gcgagtcggt ggtctcctgg
      721 gcgctggttc cctgggctgc cgccaggacg ctgccgaacg cagtgatgaa tgcgcggcgg
      781 atcggtcgtg cgtggaaagc gcgcttcatc ttgtactttt gccggatagc cgcggcggcg
      841 caatccctac atcgatcgga acaggtgcac atacgggtcg ctcacaggca aggtttcctt
      901 cctgtccttc agcttcaccc gcatgtgacc gccaatgtcg cgatggacgc cggcaattgc
      961 gttgatgttg atgaccgtcc cccgatggat ttgccagaac tgcgccggat ccagttcctg
     1021 cacgagttcc ttgatcgcgc gccgtatcag cgattcttgc tgcgcggtga cgaccagcgt
     1081 gtacttgctg tcggccttga agtagcagac ctcctcggtc gtcacaagcc gcacgttctg
     1141 cccttgcgag gcgttgatcc aacgcaggta gccgcgcttg ggcgcctgct cgcgcaaggt
     1201 gtcgagcacc gcgtcgaagc gcgcgggcgc ggcgtcgagg cgctcgcgca cgcgcgcgat
     1261 cgccgtcgcc aggcgggcgg cgctgaaggg tttcatcacg tagtcgaccg cgccctgctc
     1321 gaacgcgctg acggcgtgct ggttgtaggc ggtgacgaag acgacatggc agcggccgct
     1381 cgccgcccgg gcgacctcca ggcccgaaag gccgggcatc tcgatgtcga ggaacatcac
     1441 ctgcggcttg cagcgaccga gcgcgtcgag cgcgtcgatg ccgtcctcgg cctcggcgac
     1501 gatgttgagg ccgggccaga gccgcgtcag gctgtccttg agctcggcgc gcaggatcgg
     1561 ttcgtcctcg gcaatgacgc agtcggtcat cggctttctc ccctgggttc gagatcgtcg
     1621 agctcgagcg gtagctcgat ttgtgcccgg ctggcgagcg cagcgccgcc gtcgggcccc
     1681 gcctcgaaac gcgcgccggc gccgtacaag gcccgcagcc gctcgtcgat ctgctcgagc
     1741 aacggctggc ccgggatgcc ggcggcaaag ccgccgccgc tggtcgagac ctcgatgcac
     1801 agccgcccgg cgacgatggc gacggacacg cgcaggcgca gcgcgcccga ttcgacgccg
     1861 ccgaccgcct gctggaccag cggcagcatc accagcgcgg gcaggcgcgc ttcgcgggcc
     1921 gctccggcga tgtcgaactc gacccggcgg ccctgcgccg accggccgac gatgtccagc
     1981 cacgcttggg ccagggccgc ctccttctcg accgtcgacg aggactcgcg caagtgcggc
     2041 agcgccgcgc gcagatagac gatcaggtcc tcgagcatcg cccctgccgc ggcggcatcg
     2101 ctgcggtaga gctggcggat gcgctcgagc gtgccgaaca ggaacatcgg ctcgacccgg
     2161 gcctgcagcg cctgcagctg cgactccagg gtcttgcgcc gggcccgcgc ccgggcctgc
     2221 tcggcctcgt gctggcgccg gcgtgccgtc atcgccgtcc gccggttgac gtgaacgaag
     2281 gtcgccaggc cgccgaccag catgccgatg agcatgaggt ccaggcggtg caggaccggg
     2341 tggaacggat tgaacacgtg ggtgctgccc ggcggaagga ag
//
LOCUS       DASRDG010000539         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_83240, whole genome shotgun sequence.
ACCESSION   DASRDG010000539 DASRDG010000000
VERSION     DASRDG010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83240"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFA35_09015"
     CDS             complement(<1..184)
                     /locus_tag="VFA35_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56353.1"
                     /translation="MLALAAAVEPAAAQRGSCFVESSPAVLHGATLGAGSRREARERT
                     ERLGFTWSDANARDNRE"
     gene            complement(250..954)
                     /locus_tag="VFA35_09020"
     CDS             complement(250..954)
                     /locus_tag="VFA35_09020"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT56354.1"
                     /translation="MLLAACLGALASACAPDPAQREYLAALKGEESGMTREQQIAHLD
                     RAIALAPRRPEYHETRAIYRIDLQQWAPARADLDADIRLADRPYARYLRGLVACEVGL
                     FQAAIADFDTAIAGDSKNAQFYRGRALAKATAGDASGALRDADRLLALAPAMAESHYA
                     RGVALLALGRDREAVPEFDAALRIRPELAYVFEARALAHERLDQAELAAADHAAFVAA
                     TEKQRNCAPCRDPYRY"
     gene            complement(1048..>2345)
                     /locus_tag="VFA35_09025"
     CDS             complement(1048..>2345)
                     /locus_tag="VFA35_09025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZT56355.1"
                     /translation="VQEKLLRDPGVDVLIATIDRVLELHARKVLTFEDVEILVLDEAD
                     RMVDMGQATDLRRLLKLLPETRQTLLFSATMPAELNRLAKEALIEPVRVDFTTPQPAP
                     GITHAIYPVPNHLKIELLDRLLSRSGEVRSTIVFTRQREGADRLARQLERRGYAVATL
                     HERKSQMERERAMEDLKRGRLQIVVTTDQAARGLELAGVSHVVNFDVPPSPEDYVHRI
                     GRAGRPDAQGDAFTLMAPEEQRHLAGIERFVGRAVPRVLLPDFNYGLKPNQLQQQAIY
                     DDEQARARHAAMAKMGFAVVRQMPTTAHGGFRAVNAQGKPLVKPTPKPAAKVAARVAA
                     KSAAKSPMKLAARLTLPASRTKLATKAAVKTLPSSRTLPTVRVIGTARVASAARAASI
                     AQAPATARAIVIPKVKPAGKPATRAAVKPAAKKPTHTKK"
BASE COUNT          288 a          861 c          837 g          359 t
ORIGIN      
        1 cctcgcggtt gtcgcgggca ttcgcgtcgg accacgtgaa gccgagccgc tcggtgcgct
       61 cacgcgcctc gcgccgcgag cccgccccga gcgtggcgcc gtgcagcacg gcgggcgagc
      121 tctcgacgaa gcaactgccc cgctgcgccg ccgccggctc cacggcggcc gcgagcgcca
      181 gcacgcacaa tcccgccccg actcgtccca tggttcgtgc tacccgcatg aaccgcgtga
      241 gttcccggct cagtatcgat agggatcgcg acagggagca cagttgcgct gtttctcggt
      301 cgccgcgacg aacgctgcat ggtcggccgc agccagctca gcctggtcga ggcgctcgtg
      361 cgcgagggcc cgcgcctcga atacgtacgc cagctccggc cggatcctga gcgccgcgtc
      421 gaactcgggg acggcctcgc gatcgcggcc gagcgccagc agcgccacgc cgcgcgcgta
      481 gtgggattcc gccatcgcgg gcgcgagcgc cagcagccga tcggcatcgc gaagcgcgcc
      541 ggacgcgtcg ccagcggtcg ccttggcgag cgcgcggccg cggtagaact gggcgttttt
      601 cgagtcgccc gcgatcgcgg tgtcgaagtc cgcgatcgcc gcctggaaca ggcccacctc
      661 acaggcgacg agtccgcgca ggtagcgcgc gtacggacgg tccgcgaggc ggatgtccgc
      721 atcgaggtcc gctcgtgcgg gcgcccactg ctgcaggtcg atccggtaga tcgcgcgagt
      781 ctcgtggtac tccggccgcc ggggcgcgag cgcgatggcg cggtcgaggt gcgcgatctg
      841 ctgctcgcgc gtcatgccgg actcctcgcc cttcaatgcc gcgaggtact cgcgctgcgc
      901 gggatcgggt gcgcaggccg acgcgagcgc acccaagcag gccgccagca acaacgctcg
      961 cggtcgcgcg ggtgcgacag cggcggtcgg gttcgtggct cgacctcgtt ccgtgttccg
     1021 ctcgccgcgc gcgctcggaa gcggccgcta cttcttggtg tgcgtcggct tcttggccgc
     1081 cggcttcacc gcggcccggg tcgccggctt gcccgccggc ttgaccttgg ggatcacgat
     1141 ggcgcgcgcc gtcgccggcg cttgcgcgat gctcgccgca cgcgcggcgc tcgcgacccg
     1201 ggccgtgccg atcacccgca cggtcggcag cgtgcgcgag ctcggcagcg tcttcaccgc
     1261 cgccttggtc gccagcttgg tccggctcgc aggcagcgtc aggcgcgccg ccagcttcat
     1321 cggcgacttc gcggccgact tggcggcgac ccgcgcggcg accttcgccg ccggcttggg
     1381 agtcggcttc accagcggct tgccctgtgc gttcacggcg cggaagccgc cgtgcgcggt
     1441 ggtcggcatc tggcgcacta ccgcaaagcc catcttcgcc atcgccgcgt ggcgcgcgcg
     1501 cgcctgctcg tcgtcgtaga tggcctgctg ctgcagctgg ttcggcttga ggccgtagtt
     1561 gaagtcgggc agcagtacgc gcggtacggc acggcccacg aagcgctcga tgccggccag
     1621 gtggcgctgc tcctccggcg ccatgagcgt gaacgcgtcg ccctgcgcgt cgggcctgcc
     1681 cgcgcggccg attcggtgca cgtagtcctc gggcgacggc ggcacgtcga agttgacgac
     1741 gtgcgacacg cccgcgagct cgagcccgcg cgcggcctgg tcggtggtga ccacgatctg
     1801 cagtcgcccg cgcttcaggt cctccatggc gcgctcgcgc tccatctggc tcttccgctc
     1861 gtgcagcgtg gccacggcgt agccgcggcg ctcgagctgg cgcgccagcc ggtcggcgcc
     1921 ctcgcgctgg cgcgtgaaca cgatcgtgct tcgcacctcg cccgagcgcg acagcaaccg
     1981 gtcgagcagc tcgatcttca agtggttcgg caccgggtag atggcgtgcg tgatccccgg
     2041 cgcgggctgc ggcgtggtga agtccacgcg caccggctcg atcagcgcct ccttcgcgag
     2101 gcggttcagc tcggccggca tcgtcgccga gaacagcagc gtctggcgcg tctcgggcag
     2161 gagcttcagg agcctgcgca ggtccgtggc ctggcccatg tcgaccatgc ggtcggcctc
     2221 gtcgagcacg aggatctcga cgtcctcgaa cgtgagcacc ttgcgcgcat gcagctcgag
     2281 cacccgatcg atcgtggcga tgagcacgtc cacgccggga tcgcgcagca gcttctcctg
     2341 cacca
//
LOCUS       DASRDG010000540         4915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1115186, whole genome shotgun sequence.
ACCESSION   DASRDG010000540 DASRDG010000000
VERSION     DASRDG010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115186"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..392)
                     /gene="erpA"
                     /locus_tag="VFA35_09030"
     CDS             complement(51..392)
                     /gene="erpA"
                     /locus_tag="VFA35_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZT56356.1"
                     /translation="MDEPPVPLVFTDSAANKVKELVDEEGNPELKLRVFVQGGGCSGF
                     QYGFTFDEIVNDDDTQMSKNGVTLLIDAMSLQYLAGAEIDYKDDLQGAQFVIKNPNAT
                     TTCGCGSSFSA"
     gene            complement(482..877)
                     /locus_tag="VFA35_09035"
     CDS             complement(482..877)
                     /locus_tag="VFA35_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZT56357.1"
                     /translation="MFGKKKQPPIRTLVGEGTVIHGELRFTEGLRIDGEVLGDVAAIG
                     DGPNILVISEKARVTGKVSGGHLIINGAVNGPIRSSELLELQPKARIAGEVRYEVLEM
                     HQGATIDGELRPMGAAEKPGLKLAASNPG"
     gene            complement(898..1608)
                     /locus_tag="VFA35_09040"
     CDS             complement(898..1608)
                     /locus_tag="VFA35_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HZT56358.1"
                     /translation="MRFKLLRRRLSISAPRVIVRSHLPWPLRWAAIALMFGFSAALAL
                     WAFEFGKSIAGLDRGATEELRRLRVEVAQLRGEREKAVSVANTAESLLKTEKVVQDKL
                     ALQLKQVEADNLAMKSDLGFFERLLPAGAGEGLMIRGLQVEPQAPGHVRFQLLVMQAG
                     KTLPEFKGGYEITLAGLLDGKPWTFTPPGGAQPLQLKQYLRIEGMLEPPPAAVVKTAS
                     VRVMDASGAVKASLTVKP"
     gene            complement(1713..2105)
                     /gene="rpsI"
                     /locus_tag="VFA35_09045"
     CDS             complement(1713..2105)
                     /gene="rpsI"
                     /locus_tag="VFA35_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZT56359.1"
                     /translation="MIGIWNYGTGRRKSSVARVFIKKGTGLITVNGKTVDEYFGRQTS
                     IMVVRQPLVLTANDAAFDVKVNVHGGGESGQAGAVRHGITRALIDYDETLKSPLKQAG
                     FVTRDAREVERKKVGLHGARRRKQFSKR"
     gene            complement(2118..2546)
                     /gene="rplM"
                     /locus_tag="VFA35_09050"
     CDS             complement(2118..2546)
                     /gene="rplM"
                     /locus_tag="VFA35_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZT56360.1"
                     /translation="MKTFSAKPAEVVHEWFVIDATDKVLGRVASEVALRLRGKHKPIY
                     TPHVDTGDFIVIVNADKIRVTGNKANDKVYYRHSGFPGGIYGTKFKDMQAKHPGRAIE
                     KAVKGMLPKGPLGYAMVKKLKVYAGATHPHAAQQPKVLDI"
     gene            2773..3471
                     /locus_tag="VFA35_09055"
     CDS             2773..3471
                     /locus_tag="VFA35_09055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT56361.1"
                     /translation="MSIPDTPQAASTPRPVTTPPNWSWVPIRSLGPRHRERIAAHLLG
                     LEPGDRYLRFGYAATDAQISKYVDMLDFEQDEVFGIFNRRLELVALAHLAHAMVGAKK
                     DGPAMSEFGVSVLPSARKRGFGRRLFEHAMLHARNRGVETLLIHALSENAAMLKIARN
                     AGATVVREGSEADAWLKLPPDTFASHVDEMVEQQAAELDYRFKVHAHQVGELLSAVGE
                     VKSSLGKTAHVAEE"
     gene            3640..4452
                     /locus_tag="VFA35_09060"
     CDS             3640..4452
                     /locus_tag="VFA35_09060"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HZT56362.1"
                     /translation="MQMLPWIIALAALALLALVLVIWALKRTPKKSVELPTEWALAPR
                     PVFSTDERRVYRLLREALPHHIVLSKLPLVRFCQPTDPNEVRFWFDLLGTSHVTFAVC
                     SANGRVLAAIDLDTDRGNSRRVMQIKQSVLSACRVRYLRCPVDQLPSVAELQLLVPSA
                     NAPARGPQPASAPPAKLHQARDSLSNAVASRRAERTALWQDSTVFQDSFFAPDSRDGG
                     FGNSEFAPMPSVRSGRPARSSRDDGPDSLQGQDADEIGGVVVDSPRFGSSSR"
     gene            4481..>4915
                     /locus_tag="VFA35_09065"
     CDS             4481..>4915
                     /locus_tag="VFA35_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZT56363.1"
                     /translation="MSTDASETPYAGLSPDLVLDALDAVGLRGDGRLIQLNSYENRVF
                     QVFLEDGRVVVTKFYRPQRWSDAQILEEHAFANELAASEIPVAPVLPLSIDARSPHAE
                     RVMTIGATLAQLNVEAGGAYRYSVSARCAGRAPELEDSDTLEW"
BASE COUNT          767 a         1685 c         1592 g          871 t
ORIGIN      
        1 ccatgaaaga agccgcctcg cgggcggctt gattcagatc cgacaaagcc tcaggcagag
       61 aagctcgagc cgcagccgca ggtcgtggtc gcgttcgggt tcttgatcac gaactgggcg
      121 ccctgcaggt cgtccttgta gtcgatctcg gcgccggcga ggtactgcag gctcatcgcg
      181 tcgatcagca aggtgacgcc gttcttgctc atctgcgtgt cgtcgtcgtt gacgatctcg
      241 tcgaacgtga aaccgtactg gaaaccggag cagccgccgc cctgcacgaa gacacgcagc
      301 ttcaactcgg ggttgccttc ttcgtcgacc agttccttga ccttgttggc ggcgctgtcg
      361 gtgaacacca gcggcaccgg cggctcgtcc atcggggcaa gggattgcag gttctcagcc
      421 tggacttttt cagcggtggc gctcatgtgt gactcctgcg gcaacatgcc gacgtgatcg
      481 attatcccgg attcgacgca gcgagcttga gaccgggctt ttccgcggct cccatcggcc
      541 gcagttcacc atcaatagtg gcaccttggt gcatttcaag cacctcgtag cgaacctcgc
      601 cggcaattcg cgccttgggc tgcagttcga gcagttcgct cgagcggatc ggcccgttga
      661 ccgcgccatt gatgatcagg tgaccgcccg agaccttgcc ggtcacgcgg gccttctcgc
      721 tgatcacgag gatgttcggg ccgtcgccga tcgcggcgac gtcgccaagc acttcgccgt
      781 cgatccgcag gccttcggtg aagcgcaact cgccgtggat cacggtgccc tcaccgacca
      841 gcgtgcggat cggtggctgc ttcttcttgc cgaacatgag gcgttctcca atagcgacta
      901 tggcttgacg gtcaggctgg ctttcaccgc gccgctcgcg tccatcaccc ggaccgaagc
      961 ggtttttacc acggccgccg gcggcggctc gagcatgccc tcgatgcgca gatactgctt
     1021 cagctgcaag ggctgcgcac cgccgggcgg cgtgaaggtc cacggcttgc cgtcgagcag
     1081 gccggcgagc gtgatttcgt agccgccctt gaactcgggc agcgtcttgc cggcctgcat
     1141 cacgagcagt tggaaccgca catggccggg cgcctgcggc tcgacctgca gcccgcgaat
     1201 catcagccct tcgcccgcgc ccgcgggcag caggcgctcg aagaagccga ggtcgctctt
     1261 catcgccagg ttgtcggcct cgacctgctt gagctgcagc gcgagcttgt cctgcacgac
     1321 cttctcggtc tttagcaggc tctcggcggt gttcgccacc gagaccgcct tctcgcgctc
     1381 gccgcgcagc tgcgcgacct cgacgcgcag gcgccgcagt tcctcggtcg caccgcgatc
     1441 gagcccggcg atgctcttgc cgaactcgaa ggcccacagc gcaagcgcgg ccgagaagcc
     1501 gaacatcagc gctatcgccg cccagcgcag cggccacggc aggtggctgc gcacgatcac
     1561 gcgcggcgcg ctgatcgaca gtcggcggcg cagcagcttg aagcgcatcg gcgtgctcgt
     1621 tcctcgtgag agaggcgcga ttgtagaagc gcccagccaa agaaaagggg cgcccgaagg
     1681 cgccccgctg gcctgtcgcg aagcgtgcga aattaccgct tgctgaactg cttgcgccgg
     1741 cgcgcgccgt gcaaaccgac cttcttgcgt tcgacttcac gcgcatcgcg cgtcacgaag
     1801 ccggcttgct tcagcggcga cttgagcgtc tcgtcgtagt cgatcagggc gcgcgtgatg
     1861 ccgtggcgca cggcgccagc ctggcccgac tcgccgccac cgtgcacgtt gaccttgacg
     1921 tcgaacgcgg catcgttcgc ggtcagcacc agcggctgac gcacgaccat gatggaggtc
     1981 tggcgaccga aatactcgtc gacggtcttg ccgttgacgg tgatcaggcc ggtgcccttc
     2041 ttgatgaaga cgcgtgccac cgacgacttg cggcggccgg tcccgtagtt ccaaataccg
     2101 atcattgcgg cagctcctta gatgtccagc actttgggct gctgagcggc atgcgggtgc
     2161 gtggcaccgg cgtagacctt cagcttcttc accatcgcgt agcccagcgg acccttcgga
     2221 agcatgccct tgacggcctt ctcgatggcg cggccagggt gcttggcctg catgtccttg
     2281 aacttggtgc cgtagatgcc gcccgggaaa cccgaatggc ggtagtacac cttgtcgttg
     2341 gccttgttgc cggtgacgcg gatcttgtcg gcgttgacga tgacgatgaa gtcgccggtg
     2401 tcgacgtggg gcgtgtagat gggcttgtgc ttgccgcgca ggcggagggc gacttcactg
     2461 gccacccgtc cgagaacctt atcggttgcg tcaatcacaa accactcatg caccacttcg
     2521 gccggtttgg cgctgaaggt tttcatgttg agtgagttgg tttgtcagcc ccgtcttgcg
     2581 ccgcgctcgg ttaccacttc ttcgaacggt ggactcttgc gacggccgtc ttccccaggg
     2641 gcaatttcgg gaaagcctgc gagtgtagca gagtcgcgtc ggcgtcgccg tgttgcagcg
     2701 caacaattcg ggagcgccac ctagccgaat cacgtagact ggggttaacc ctgaacccag
     2761 gagacctcgg caatgagcat tcctgacacc ccccaagcgg cctcgacgcc gcgccctgtg
     2821 accacgcccc cgaactggag ctgggtgccg atccgctcgc tcgggccacg ccaccgcgag
     2881 cgcattgccg cccacctgct cggcctcgaa ccgggcgacc gctaccttcg cttcggctat
     2941 gcggccaccg atgcgcagat ctccaagtac gtcgacatgc tcgacttcga gcaggacgag
     3001 gtgttcggca tcttcaaccg gcggctcgag ttggtggcgc tggcacacct ggcgcacgcg
     3061 atggtcgggg cgaagaagga cggcccggcg atgtcggagt tcggcgtctc ggtgctgccc
     3121 agcgcgcgca agcgcggctt cggtcggcgc ctgttcgagc acgcgatgct gcatgcgcgc
     3181 aaccgcggcg tcgagacgct gctgatccac gcgctgagcg agaacgcggc aatgctgaag
     3241 atcgcccgca acgccggcgc gaccgtcgtg cgcgagggct ccgaggcgga tgcgtggctg
     3301 aagctgccgc ccgacacctt tgcctcgcac gtcgacgaga tggtcgagca gcaggccgcc
     3361 gagctcgact accggttcaa ggtgcacgca caccaggtcg gcgagttgct gagcgcggtc
     3421 ggcgaggtca aaagctcgct cggcaagacc gcgcacgtcg cggaagagtg acgccggcgc
     3481 gtgcattccg cacgcgccgc ggcgtcgcgg ccggcgcacc cgcatgtcgg gggatccccc
     3541 caagcttgcc gtccggttca catccgttac agtgcctgat cgggcccgtc gcttgcggcg
     3601 tgcccccccg gccttccgaa cccgcgctgt cgtccgacca tgcaaatgct tccgtggatc
     3661 atcgcgctcg ccgccctggc gctgctggcc ctcgtgctgg tgatctgggc cctgaagcgc
     3721 acgccgaaaa agtcggtgga gttgccgacc gagtgggcac tcgccccgcg tccggtgttc
     3781 tcgaccgacg aacgccgcgt ctaccgcctg ctgcgcgaag cgctgccgca tcacatcgtg
     3841 ctgtcgaaac tgccgctggt gcgcttttgt cagccgaccg acccgaacga agtgcgcttc
     3901 tggttcgacc tgctgggcac cagtcacgtg accttcgcgg tgtgcagcgc gaacggccgc
     3961 gtgctcgccg cgatcgacct cgacaccgac cgcggcaact cgcgccgcgt gatgcagatc
     4021 aagcagtcgg tgctcagcgc ctgccgcgtg cgctacctgc gctgcccggt cgaccagttg
     4081 ccgtccgtgg ccgagctgca actgctcgtg ccgagcgcga acgcaccggc gcgcggaccg
     4141 cagccggcct cggcgccgcc cgccaaactg caccaggcgc gcgattcgct ctcgaacgct
     4201 gtcgcgtcgc gccgcgccga gcgcaccgcg ctgtggcagg actcgaccgt gttccaggac
     4261 tcgttcttcg ctcccgactc gcgcgacggc ggcttcggca acagcgagtt cgctccgatg
     4321 ccgagcgtgc gctccggacg ccccgcgcgc tccagccgcg acgacggccc cgattcgctg
     4381 caaggccagg acgccgacga aatcggcggc gtggtcgtcg acagcccgcg cttcggctcc
     4441 agctcgcgct gacgcgccag ctctcgcata ggatcggtgc atgagcaccg acgccagcga
     4501 gactccttat gcggggctca gccccgatct cgtgctggat gcgctcgacg ccgtcggcct
     4561 gaggggcgac ggtcggctga tccagctcaa ttcgtacgag aaccgcgtgt tccaggtgtt
     4621 tctggaggac ggtcgcgtcg tcgtcacgaa gttctatcgg ccgcagcgtt ggagcgacgc
     4681 gcagatcctc gaggagcatg cgttcgcgaa cgagctcgcg gccagcgaga ttccggtcgc
     4741 gccggtcctg ccgctgagca tcgacgcgcg ctcgccgcac gccgagcgcg tgatgacgat
     4801 cggcgcgacg ctcgcgcagc tcaacgtcga agccggcggc gcctaccgct acagcgtcag
     4861 cgctcgctgc gccggccgcg cacccgaact cgaagactcc gacacgctcg aatgg
//
LOCUS       DASRDG010000541         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1235248, whole genome shotgun sequence.
ACCESSION   DASRDG010000541 DASRDG010000000
VERSION     DASRDG010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235248"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /gene="ssb"
                     /locus_tag="VFA35_09070"
     CDS             <1..449
                     /gene="ssb"
                     /locus_tag="VFA35_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZT56364.1"
                     /translation="GSAICNVTVATTRQWKNKESGERQEETDWHRVVFFDRMAEIAGE
                     YLKKGRSVYVEGRLRTRKWTDKEGKDQYTTEIVATDMQMLGSREGMGGGAGSGGGGGD
                     EEGGGYERSAPPQRSAPSSRPQASAPRAAPKPAATGFDNMDDDIPF"
     gene            complement(486..1190)
                     /locus_tag="VFA35_09075"
     CDS             complement(486..1190)
                     /locus_tag="VFA35_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56365.1"
                     /translation="MRNSIDFSSWQGILSTLLGLVLVSLVAVGIRLLVMHSVQQRRER
                     QNRQINERLKTLIAAYKVLGGSFTGDLGVDPSHLRELRARDADDATAGAHDDSAARAE
                     ANGSDRRRRIRDAVEAALSDVILLGTEAQVRLAVQAARDMVAGRRVETAELVVSLRSF
                     IREVLDLEAIPADWAVPKQGPVRAGGGSSARGGRAGGATGGRGGEGGGGGGRGGGMNP
                     GDPLDPTGGPDHPAHA"
     gene            1294..1542
                     /locus_tag="VFA35_09080"
     CDS             1294..1542
                     /locus_tag="VFA35_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HZT56366.1"
                     /translation="MNDTTQAPPIPDRLSIDPRSPHHVAAVFAHEVGIRLNDKERFDV
                     EEYCISEGWVKVPAGKTLDRKGRPLMIKLKGRVEAFYR"
     gene            complement(1559..>2472)
                     /locus_tag="VFA35_09085"
     CDS             complement(1559..>2472)
                     /locus_tag="VFA35_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZT56367.1"
                     /translation="YPNYTRITRADADAIHAYLRSLPPVVQPNKPHELAFPFDQQAAL
                     AVWRALYFRAAEPARPDADTARSAEWRRGAYLVEGLGHCNACHASRNALGATASTLDL
                     AGGLIPVQNWYAPSLTSPDEAGVAAWELPQIVALMRTGVSARGAVMGPMREVVAGSTQ
                     YLTEPDLRAMATYLKALPQTPADALPQDAPPGPAADGPGAKLYTQYCAQCHGEQGEGV
                     PGIYPTLAGSRAVTMRTPVNLVHIVLEGGFAPSTAGNPRPYGMPPFATLLGDAEVAQL
                     LSHLRGSWGNRAAPVSALEVGQFRGVK"
BASE COUNT          352 a          879 c          921 g          320 t
ORIGIN      
        1 gcggcagcgc gatctgcaac gtcaccgtcg cgaccacgcg gcagtggaag aacaaggagt
       61 ccggcgagcg ccaggaagag accgactggc accgcgtcgt gttcttcgac cgcatggccg
      121 agatcgccgg cgagtacctg aagaagggcc gttcggtcta cgtcgaaggc cgcctgcgca
      181 cgcgcaagtg gaccgacaag gaaggcaagg accagtacac caccgagatc gtcgcgaccg
      241 acatgcaaat gctcggcagc cgcgaaggca tgggcggcgg cgcgggcagt ggcggtggcg
      301 gtggcgacga agaaggcggc ggctatgagc gcagcgcgcc gccgcagcgc tcggcaccgt
      361 cctcgcgccc gcaagcgtca gcgccgcgcg ccgcgccgaa accggcagcg accggcttcg
      421 acaacatgga cgacgacatt ccgttctgac ggccctggcc ccggccccgg cgccgcgccg
      481 cgggcctatg cgtgtgctgg gtggtccggc ccgccggtcg ggtcgagcgg gtcgccgggg
      541 ttcatcccgc cgccgcggcc accgcctcct ccaccctcgc cacccctgcc gccggtcgca
      601 ccgcccgcgc ggccgccgcg tgccgacgat ccgccgccgg cgcgcaccgg accttgcttc
      661 ggcaccgccc aatcggccgg gatcgcctcc agatcgagca cctcgcgaat gaagctgcgc
      721 agcgagacga ccagttcggc cgtctcgacg cggcggcccg cgaccatgtc gcgcgccgcc
      781 tgcaccgcca ggcgcacctg cgcctcggtg ccgagcagga tcacgtcgga gagcgcggcc
      841 tcgaccgcgt cgcggatgcg gcggcgccgg tccgaaccgt tggcctcggc tcgcgcggcc
      901 gagtcgtcgt gcgcgccggc cgttgcgtcg tcggcatccc gcgcacgcaa ctcgcgcaag
      961 tggctcgggt cgacgccgag atcgccggtg aacgagccgc cgagcacctt gtaggccgcg
     1021 atcagcgtct tcaggcgttc gttgatctgg cggttctgcc gctcgcgccg ctgctgcacg
     1081 ctgtgcatca cgagcagccg gatgccgacg gccacgagcg agacgagcac caggccgagc
     1141 agcgtcgaca ggatgccttg ccaggagctg aagtcgatgg agttgcgcaa ggggggcggc
     1201 gggacgagtc gcggcgatca tacggcgggt tcgcggccgc cggggcggcg tgcgccaccc
     1261 cgcggcgcgc cgaagatgcg ggaaaatcgc cgcatgaacg acaccaccca agcgccgccg
     1321 atccccgacc gcctctcgat cgacccgcgc agcccgcacc acgtcgcggc cgtgttcgcg
     1381 cacgaggtcg gcatccgcct gaacgacaag gagcgcttcg acgtcgagga atactgcatc
     1441 agcgaaggct gggtcaaggt gcccgcgggc aagacgctcg atcgcaaggg ccggccgctg
     1501 atgatcaaac tgaaggggcg ggtcgaggcg ttctaccgct gagcgatccg ctgcgccgct
     1561 atttcacgcc gcggaactgc cccacctcga gcgccgagac cggcgcggcg cggttgcccc
     1621 agctgccgcg cagatggctc agcaactgag cgacctcggc gtcgccgagc agggtcgcga
     1681 acggcggcat gccgtagggg cgcgggttgc cggcggtgct gggcgcgaag ccgccttcga
     1741 gcacgatgtg gaccaggttg accggcgtgc gcatcgtgac ggcgcggctg ccggccagcg
     1801 tcgggtagat gcccggcacg ccttcgccct gctcgccgtg gcactgcgcg cagtactgcg
     1861 tgtagagctt ggcgccgggg ccgtccgccg cggggccggg cggcgcgtct tgtggcagcg
     1921 cgtccgccgg tgtctgcggc agcgccttca ggtaggtcgc catcgcgcgc aggtcgggtt
     1981 cggtcaggta ctgggtgctg ccggcgacga cctcgcgcat cggccccatc accgcgccgc
     2041 gcgccgagac gccggtgcgc atcagcgcga cgatctgcgg cagctcccac gcggcgacgc
     2101 cggcttcgtc gggtgacgtg agcgacggcg cgtaccagtt ctgcaccggg atcaggccgc
     2161 cagcgaggtc gagcgtgctc gcggtcgcgc cgagcgcgtt gcggctcgcg tggcaggcgt
     2221 tgcagtggcc caggccctcg acgaggtagg cgccgcgccg ccactcggcg ctgcgggcgg
     2281 tgtcggcgtc gggccgcgcc ggctcggcgg cgcggaagta gagcgcgcgc cagaccgcga
     2341 gcgcggcctg ctggtcgaac gggaacgcca gttcgtgcgg cttgttcggc tgcaccaccg
     2401 gtggcaagct gcgcaggtag gcgtggatcg cgtcggcgtc tgcgcgcgtg atgcgggtgt
     2461 agttcgggta cg
//
LOCUS       DASRDG010000542         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_443938, whole genome shotgun sequence.
ACCESSION   DASRDG010000542 DASRDG010000000
VERSION     DASRDG010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443938"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1094)
                     /locus_tag="VFA35_09090"
     CDS             complement(30..1094)
                     /locus_tag="VFA35_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZT56368.1"
                     /translation="MSTAHAFAELGLAPDASELEVKAAWRRLVSQWHPDRNDSADAVA
                     TMQRINKAFEAIRRSAFNGAGAPPADDVDDGDAAPAHPAAEPDRQPRRTINRKVKLTL
                     EEAALGCTKVLHGKVTDTCQPCAGAGYRTLGSACSRCKGAGSLRQRAWFGWLDTLAEC
                     EACAGSGVVRPPCPACDGTGKSAPRRYRFNVRIPHGVRDGDLLHVDGRRPGPGQRPGD
                     DDLDLRVEIEEHAFFRLDDDGTIRCTVPVDGFAWIANRPIDVPTLGGTQRLPLSRDRL
                     LYRLAGQGFPVERRGRCGDQLVELVPIFPERLGADQEILIDQLIASSAGPNGRAADAR
                     LRAWNRELRAWQRGQPTGEG"
     gene            complement(1107..1370)
                     /gene="minE"
                     /locus_tag="VFA35_09095"
     CDS             complement(1107..1370)
                     /gene="minE"
                     /locus_tag="VFA35_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HZT56369.1"
                     /translation="MSLLSFLLGEKKKTASVAKERLQIILAHERSGRGGSRPDYLPAL
                     QKELMAVISKYVSIKAEDIKVHLEKQDNLEVLQVKIELPEAAR"
     gene            complement(1373..2188)
                     /gene="minD"
                     /locus_tag="VFA35_09100"
     CDS             complement(1373..2188)
                     /gene="minD"
                     /locus_tag="VFA35_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HZT56370.1"
                     /translation="MAKIVVVTSGKGGVGKTTTSASFASGLALAGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQDEANLNQALIKDKQCDNLFVLAASQTRDKDALTQDGV
                     EKVLNDLAAMGFDYIVCDSPAGIETGALMAMHFADEALVVTNPEVSSVRDSDRILGML
                     ASKTKRAIDGKEPIKEHLLITRYNPNRVEGGQMLSLGDIKDILRIELIGVIPESETVL
                     DASNQGVPAVHLKGTDVSEAYKDVVARFLGETRPLRFTDAVKPGFFKRVFGGR"
     gene            complement(2211..2993)
                     /gene="minC"
                     /locus_tag="VFA35_09105"
     CDS             complement(2211..2993)
                     /gene="minC"
                     /locus_tag="VFA35_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HZT56371.1"
                     /translation="MAVAIPASSAPAPSGAPTAFELKSAALTLVSVVLKTTDLARLSQ
                     ALDERFGDAPELFDNDPVAIDLSFIRDAAEPIDFVVLIHLLRGHKMLPVAVKAGSLQQ
                     MEDARAAGLAEGHGSAAAAAPPARAAKAPAAEPAKPVEVIAEAPPARPTLIVDKPLRS
                     GQRVYARGGDLVVLAVVSFGAEVIADGNIHVYAPLRGRALAGALGDTSARIFTTCMAP
                     QLLSIAGIYRTTETELPAAVLSKPAQVRLAGAKLVVEPLKSD"
     gene            complement(3129..>3589)
                     /locus_tag="VFA35_09110"
     CDS             complement(3129..>3589)
                     /locus_tag="VFA35_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT56372.1"
                     /translation="LNAAVLKLSAAPDANRLRRDGLRWIPKANGEFKIPVVSIHTLGD
                     LFVPFGMEQVYQKRVAAKGNSAWLVQRAIRGVSHCDFTVAEQVSAFDDMIKWEGGAAK
                     PPGDDVVTPATVAAASYGCAFTNNAMPIPGDESGTTKALRPATVAQRPCP"
BASE COUNT          491 a         1233 c         1283 g          582 t
ORIGIN      
        1 ctgccccgag gcgtagcgct tcgcgccggc tacccctcgc ccgtcggctg cccccgctgc
       61 caggcacgca gttcccgatt ccacgcgcgc agccgggcgt ccgcggcgcg cccgttcggc
      121 ccggccgacg aggcgatgag ctggtcgatc aggatctcct ggtcggcacc gagccgctcg
      181 ggaaagatcg gcacgagctc caccagttga tcgccgcacc ggccccggcg ctccaccggg
      241 aagccttggc ccgccaggcg gtacagcagc cggtcgcggc tcaagggcag gcgctgcgtg
      301 ccgccgagcg tcggcacgtc gatcggccgg ttggcgatcc aggcgaagcc atcgaccggg
      361 acggtgcagc ggatcgtgcc gtcgtcgtcg agccggaaga acgcgtgctc ctcgatctcg
      421 acgcgcaggt cgaggtcgtc gtcgcccggg cgctggccgg ggcccgggcg gcgcccatcg
      481 acgtggagca ggtcgccgtc acgcaccccg tgcgggatgc ggacgttgaa ccggtaacgc
      541 cgcggcgccg acttgcccgt gccgtcgcag gccgggcacg gcggccgcac gaccccgctg
      601 ccggcgcagg cctcgcactc ggccagcgta tcgagccagc cgaaccaggc gcgctgccgc
      661 agcgaccccg cgcccttgca tcgcgagcac gccgaaccga gcgtgcgata gccggcgccg
      721 gcgcagggct ggcaggtgtc ggtgaccttg ccgtgcagca ccttggtgca gccgagcgcc
      781 gcctcttcga gcgtcagctt gaccttgcgg ttgatcgtcc ggcgcggctg ccggtccggc
      841 tcggccgcgg gatgtgccgg ggcggcgtcg ccgtcatcga catcgtcggc aggcggcgcg
      901 ccggctccgt tgaacgcgga gcgacggatc gcttcgaagg ccttgttgat gcgctgcatc
      961 gtcgcgacgg catcggcgct gtcgttgcgg tcggggtgcc actgcgagac caggcggcgc
     1021 cacgcggcct tgacctcgag ctcgctcgca tcgggggcca ggccgagctc ggcaaaagca
     1081 tgggcggtac tcaagggcgg cctggctcag cgcgccgcct cgggcagctc gatcttgacc
     1141 tgcagcacct cgaggttgtc ctgcttctcc aggtgcacct tgatgtcttc ggccttgatc
     1201 gagacgtact tcgagatcac cgccatcagc tctttctgca gcgccggcag gtagtcgggg
     1261 cggctgccgc cgcgcccgct gcgctcgtgc gcgaggatga tctgcagccg ctccttggcg
     1321 acgctggcgg tcttcttctt ctcaccaagc aggaaagaga gcagcgacat gcttacctcc
     1381 cgccgaacac gcgcttgaag aagccgggct tgaccgcgtc ggtgaagcgc agcggccgcg
     1441 tctcgccgag gaaacgcgcg accacgtcct tgtaggcctc cgacacgtcg gtgcccttca
     1501 ggtgcacggc cggtacgccc tggttcgagg cgtcgagcac ggtctcggat tcggggatca
     1561 cgccgatcag ctcgatgcgc aggatgtcct tgatgtcgcc cagcgacagc atctgcccgc
     1621 cctcgacccg gttcgggttg tagcgcgtga tcaagaggtg ctccttgatc ggctccttgc
     1681 cgtcgatcgc gcgcttggtc ttcgacgcca gcatgccgag gatgcgatcg gagtcgcgca
     1741 ccgatgagac ctcggggttg gtgacgacca gcgcctcgtc ggcgaagtgc atcgccatca
     1801 gcgcgccggt ctcgatgccg gccggcgagt cgcagacgat gtagtcgaag cccatcgcgg
     1861 ccaggtcgtt cagcaccttc tcgacgccgt cttgcgtcag tgcgtccttg tcgcgcgtct
     1921 gcgaggcggc gagcacgaac aggttgtcgc actgcttgtc cttgatcagc gcctggttca
     1981 ggttggcctc gtcctggatc acgttgatca ggtcgtagac gacgcggcgc tcgcagccca
     2041 tgatcaggtc gaggttgcgc aggccgacgt cgaagtcgat caccgcggtc ttgtggccgg
     2101 ccagcgccag gccggaagcg aagctggcgc tggtggtggt cttgcccacc ccgcccttgc
     2161 cggaggtcac gacgacgatc ttggccatgg tctcgggttt ccttgtgtgt tcaatcggat
     2221 ttcaatggct cgacgacgag cttggcgccg gcgagccgca cctgtgccgg cttgctcagc
     2281 acggcagcgg gcagttcggt ctcggtggtg cggtagatgc cggcgatgga cagcagctgc
     2341 ggcgccatgc aggtcgtgaa gatgcgcgcg ctcgtgtcgc cgagcgcgcc ggcgagcgcc
     2401 cgcccgcgca gcggcgcgta gacgtggatg ttgccgtcgg cgatcacctc ggcgccgaag
     2461 ctgacgacgg cgagcacgac caggtcgccg ccccgcgcgt agacgcgctg gccggagcgc
     2521 agcggcttgt cgacgatcag tgtcgggcgt gcgggcggcg cttcggcgat cacctcgacc
     2581 ggcttggccg gctcggccgc aggcgctttc gcggcgcgcg ccggcggcgc tgcggcggcg
     2641 gcgctgccgt gcccttcggc caggcccgcg gcgcgcgcgt cttccatctg ctgcaggctg
     2701 cccgccttca ccgcgaccgg cagcatcttg tggccgcgca gcaggtggat cagcacgacg
     2761 aagtcgatcg gctcggcagc gtcgcgtatg aacgagagat cgatcgcgac ggggtcgttg
     2821 tcgaacagct ccggcgcatc gccgaagcgc tcgtcgagcg cctgcgacag gcgcgcgagg
     2881 tcggtcgtct tcagcaccac cgagaccagc gtgagcgcgg cgctcttcaa ctcgaacgcg
     2941 gtgggagcgc cggaaggcgc gggggcggaa gacgcgggaa tagcaacagc catggacgca
     3001 gacggcggca aaggcccgaa gtttaccggg cgtcccgggc gatccggttt gcaagtgtgt
     3061 gctagggcgc tgtcggcgcg atgagcaagg ggcccgaagg ccccctgcgt gtcaggtgtc
     3121 gtgtcgtctc aggggcacgg ccgctgcgcg accgtggccg gacgcagcgc cttggtcgtg
     3181 ccgctctcgt cgcccgggat cggcatcgcg ttgttcgtga aggcgcagcc gtagctcgcg
     3241 gcggcgaccg tcgccggcgt caccacgtcg tcgccgggcg gcttggcggc gccgccttcc
     3301 cacttgatca tgtcgtcgaa cgcgctgacc tgctcggcga ccgtgaagtc gcagtggctc
     3361 acaccgcgga tcgcgcgctg caccagccat gcgctgttgc ccttggcggc gacgcgcttc
     3421 tggtaaacct gctccatgcc gaacggcacg aacaggtcgc ccagcgtatg gatcgagacg
     3481 accgggatct tgaactcgcc gttcgccttc gggatccagc gcaggccgtc gcggcgcagg
     3541 cggttcgcat cgggcgcggc gctcagcttg agcaccgcgg cgttcagcg
//
LOCUS       DASRDG010000543         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1596021, whole genome shotgun sequence.
ACCESSION   DASRDG010000543 DASRDG010000000
VERSION     DASRDG010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596021"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="VFA35_09115"
     CDS             <1..1578
                     /locus_tag="VFA35_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT56373.1"
                     /translation="MALYIERICSNAEVYLNGERIFSGGRMGEPVTRNCHYPQLITLP
                     PALLQERGNTLDVHLHGHALPRVASRLRVGGLSAIQVGPQAQLAQAYDERLFWNVTAV
                     QLVNLALAVLGCVMVGMGWMNRREAYLGYFGWLCLAWGLLSARVWWRDLPWDNGVTEF
                     LFCTGFAPLVALAVQFLLSYAGLRSRWIENALAAQWVLLPATLLLAGPGRLFLMASIW
                     YAVLVAEVIAAIGLHLVVSRRAKRADFWSMTALLSAALVLLGVQLAIEYGLVPNPGIP
                     VLQLALPLLFIGMAARLLQVFARALRAAEAGRENLEGRVQEITAEIERNFAQLAELRV
                     EQVTERERKRIAADLHDDLGAKLLTIVHTSESERISTLARDALEEMRLSVRGLTGKPM
                     RLADALADWRAETVSRLGQANIEADWRGPTEEIEQLLPARGFVQTTRILREAVSNIIK
                     HSGASHCKVRCAISDRQFGLTVQDNGKGIPMELDGKLDRGHGMSSMKNRAKQMQGQCL
                     VESGPGYGTVIRLTVPL"
     gene            1676..2332
                     /locus_tag="VFA35_09120"
     CDS             1676..2332
                     /locus_tag="VFA35_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZT56374.1"
                     /translation="MTNILLLEDLPEIRAWLKVLIKGVFPEAQIHEAARVHDALELVT
                     AVKFNLALCDLGLPDGSGVDVVAALRENQPECQSVVVTIHDDDDHLFPALQAGAFGYL
                     LKEQARELIAEQLKRMSQGEPPLSPSIARRVISHFAAQTRQQERPQNMPHVALTDREN
                     EVLLRVAKGFTLPEIGVQLNLSRHTIADYVKQIYRKLNVSSRAEAALEAQRLGLFRRS
                     "
     gene            2358..>2784
                     /gene="ruvA"
                     /locus_tag="VFA35_09125"
     CDS             2358..>2784
                     /gene="ruvA"
                     /locus_tag="VFA35_09125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZT56375.1"
                     /translation="MIGRITGTLAEKSPPQLLIDVNGVGYEVDVPMSSFYNLPGLGER
                     VTLLTHFVVREDAQVLFGFLSHEERSTFRLLVKISGVGPRTALSILSGLSVDELARAV
                     GLQDSGRLIKVPGIGKKTAERLLLELKGKLADAVAAPASV"
BASE COUNT          433 a          966 c          962 g          423 t
ORIGIN      
        1 atggcgctgt acatcgagcg catctgcagc aacgccgagg tctacctgaa cggcgagcgc
       61 atcttcagcg gcggccgcat gggcgagccg gtgacgcgca actgccacta cccgcagctg
      121 atcacgctgc cgcccgcgct gctgcaggag cgcggcaaca cgctcgacgt gcacctgcac
      181 ggccacgcgc tgccccgcgt cgcgtcgcgt ttgcgcgtcg gtgggctctc ggcgatccag
      241 gtcggcccgc aggcgcagct ggcgcaggcc tacgacgagc gcctgttctg gaacgtgacc
      301 gcggtgcagc tcgtcaacct cgcgctcgcg gtgctcggct gcgtgatggt cggcatgggc
      361 tggatgaacc ggcgcgaggc ctacctcggc tacttcggct ggctgtgcct ggcgtggggg
      421 ctgctctcgg cgcgcgtgtg gtggcgcgac ctgccgtggg acaacggcgt gacggagttc
      481 ctgttctgca ccggcttcgc gccgctggtc gcgctggcgg tgcagttcct gctcagctat
      541 gccgggctgc gctcgcgctg gatcgagaac gcgctggccg cgcaatgggt gctgctgccg
      601 gccacgctgc tgctcgccgg cccggggcgc ctgttcctga tggcgagcat ctggtacgcg
      661 gtgctggtgg cggaggtgat cgccgcgatc ggactgcacc tggtcgtcag ccggcgcgcc
      721 aagcgggcgg atttctggtc gatgaccgcg ctgctgagcg cggccctggt gctgctgggc
      781 gtgcagctcg cgatcgagta cgggctggtc ccgaacccgg gcatcccggt gctgcagctc
      841 gcgctgccgc tgctgttcat cggcatggcg gcgcggttgc tgcaggtgtt cgcgcgcgcg
      901 ctgcgcgccg ccgaagccgg ccgcgagaac ctcgaaggcc gggtccagga gatcaccgcc
      961 gagatcgagc gcaacttcgc ccagctcgcc gagctgcgcg tcgagcaggt caccgaaaga
     1021 gaacgcaagc gcatcgccgc cgacctgcac gacgacctcg gtgccaagct gctgacgatc
     1081 gtgcacacga gcgaatccga acgcatctcg acgctggcgc gcgacgcgct cgaagagatg
     1141 cgcctgtcgg tgcgcggcct gaccggcaag ccgatgcggc tggccgacgc cctggccgac
     1201 tggcgcgccg aaaccgtctc gcgcctcggc caggccaaca tcgaggccga ctggcgcggg
     1261 ccgaccgaag agatcgagca gttgctgccg gcgcgcggct tcgtgcagac cacgcgcatc
     1321 ctgcgcgagg ccgtcagcaa catcatcaag cacagcggcg cctcgcactg caaggtgcgc
     1381 tgcgcgatca gcgaccgcca gttcgggctg acggtgcagg acaacggcaa gggcatcccg
     1441 atggagctcg acggcaagct cgaccgcggc cacggcatgt cgagcatgaa gaaccgcgcc
     1501 aagcagatgc aggggcagtg cctggtcgag tccggccccg gctatggcac tgtgataagg
     1561 ctcacggttc cactgtgaac cgtgccggcc caggcacacc ggccgctggt acgatgaaaa
     1621 gaggcaccgt ttcgcgtgcc ggcctgcggg ctctgacgac catcgaggcg ctgccatgac
     1681 caacatcctg ctgctcgaag acctgcccga aatccgcgca tggctgaagg tgctgatcaa
     1741 gggcgtgttc ccggaggcgc agatccacga ggccgcgcgc gtgcacgacg cgctcgagct
     1801 cgtcaccgcg gtcaagttca acctcgcgct gtgcgacctc ggcctgcccg acggttcggg
     1861 cgtcgacgtg gtcgcggcgc tgcgcgagaa ccagcccgag tgccagtcgg tggtggtcac
     1921 gatccacgac gacgacgacc acctgttccc ggcgctgcag gccggcgcct tcggctacct
     1981 gctgaaggag caggcgcgcg agctgatcgc cgagcagctc aagcgcatga gccagggcga
     2041 gccgccgctc tcgccgtcga tcgcacgccg cgtgatctcg cacttcgcgg cccaaacgcg
     2101 ccagcaggag cggccgcaga acatgccgca cgtggcgctg accgaccgcg agaacgaggt
     2161 gctgctgcgc gtcgccaagg gcttcacgct gcccgagatc ggcgtgcagt tgaacctgtc
     2221 gcgccacacg atcgccgact acgtgaagca gatctaccgc aagctcaacg tcagctcgcg
     2281 tgccgaagcc gcgctggagg cacagcgcct gggcctgttc cgccgctcct gaatggggtg
     2341 acaccgataa tctggtgatg atcggacgca tcaccggcac gctcgcggaa aaatcgcccc
     2401 cgcaattgct catcgacgtg aacggcgtcg gctacgaggt cgatgtgccg atgtcgagct
     2461 tctacaacct gcccggcctc ggcgagcgcg tcaccctgct gacccacttc gtcgtgcgcg
     2521 aggacgcgca ggtgctgttc ggcttcctca gccatgagga gcgctcgacc tttcgcctcc
     2581 tcgtcaagat ctcgggcgtc ggcccgcgca cggccctctc gatcctctcc ggtctctcgg
     2641 tcgacgagct ggcgcgcgcc gtcggcctgc aggacagcgg ccggctgatc aaggtgccgg
     2701 ggatcggcaa gaagaccgca gagcggctcc tgctcgagct caagggcaag ctcgccgacg
     2761 ccgtcgccgc gcccgcgagc gtga
//
LOCUS       DASRDG010000544         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1596078, whole genome shotgun sequence.
ACCESSION   DASRDG010000544 DASRDG010000000
VERSION     DASRDG010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596078"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..700
                     /locus_tag="VFA35_09130"
     CDS             47..700
                     /locus_tag="VFA35_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HZT56376.1"
                     /translation="MQPIVHPSAPTFDDEVAQRLGMLLTRRSVSPKRLGPPSPTPSEL
                     EPMLQAALRAPDHGGLHPWRVIEFRSEARAALATIFEEEKRRRDPLASPTEWARAREH
                     ATRAPMLLGFVVSPKHGASVPLREQWLAAGAALCNLLNAAHQLGFGAIMLSGERCHDR
                     TLRSQLGVLAEEFLAGFVSIGRIAQAPPERQPVASSLVWSSWAPETDAAGCADQSCA"
     gene            complement(733..>2673)
                     /locus_tag="VFA35_09135"
     CDS             complement(733..>2673)
                     /locus_tag="VFA35_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HZT56377.1"
                     /translation="LALVLPLLGLLLLAWANVAFDRLLITKVRSDLAVARGYFDRVLV
                     EVGASTGAVADSHALHVALAARPAAELVALLRDVKAREGLDFINLRDTDGALLVTDAG
                     APADAGQRPPALRAAAHGRPSASIEILQPGELVGVAPALGARLSVPLVATRNAAPTTR
                     AAEDRAMVVLATAPVRDGEGHLLGHVQAGVLLNRNLPFIDHINEIVYPEGALPFGSRG
                     TATLFLDDVRISTNVRLFGSESNDRAIGTRVSQAVRDAVLGHGDTWLDSAFVVDNWYV
                     SAYQPLLDGAGQRVGMLYVGFLERPFTLLKYGMLAAIGLIFFTVMLVSANVSLRGARS
                     IFRPIELMAQTMRRVESGAPDARVGELANRDEIGQLAGHLDHLLDVVDENTHALQRWN
                     AELDAKVVERTRDLEIAQQQLVRSEKLAAVGQLTASIAHEVNNPIAVIQGNLDLVRDL
                     IGPEAAKAVRSELALVDEQIERMRLIVTQLLQFARPDEYAGYIDNVDTEAELEGCLVL
                     VGHLLAKTHIHIERDLRATRRPAINRNELQQVLVNLLVNAIHTMQGGGTLRLASRDVA
                     DDGVEIEIADTGPGLGPELQAELFKPFVTRKKDGTGLGLWISRTIVERYGGDIRAADR
                     SDGVSGAVFTVALRREAAIDEA"
BASE COUNT          385 a          969 c          919 g          400 t
ORIGIN      
        1 cgggccgatc atgtcggcct ctctcgggca ccgtgatcga cctcgcatgc aacccatcgt
       61 ccaccccagc gccccgacct tcgacgacga agtcgcgcag cgcctgggca tgctcttgac
      121 ccgccgctcg gtctcgccga agcgcctcgg tccgccgagc cccaccccgt ccgagctcga
      181 accgatgctg caagcggcgc tgcgcgcgcc cgatcatggc ggcctgcacc cgtggcgcgt
      241 catcgaattc cgcagcgaag cgcgcgccgc attggcgacg atcttcgaag aagaaaagcg
      301 ccggcgcgac ccgctggcat cgcccaccga gtgggcccgg gcccgcgagc acgcgacgcg
      361 cgccccgatg ctgctgggct tcgtcgtctc gcccaagcac ggcgccagcg tgccgctgcg
      421 cgagcagtgg ctcgccgcgg gcgcggcctt gtgcaacctg ctcaatgcgg cccaccaact
      481 cggcttcggc gccatcatgc tcagcggcga gcgttgccat gaccgcacgc tgcgatcgca
      541 actcggcgtg ctggccgagg agttcctcgc cggcttcgtc agcatcggcc gaatcgcgca
      601 ggccccgccg gagcggcaac ccgtcgcctc cagcctcgtc tggtcgagtt gggccccgga
      661 aacggacgcg gcgggctgcg ctgatcagtc ctgcgcctga ccgaagcgcg gtcactgggc
      721 gaccctgcgc gctcacgctt cgtcgatcgc ggcctcccgg cgcagcgcca ccgtgaacac
      781 cgcgccgctc acgccgtcgc tgcgatcggc cgcgcgaatg tcgccgccgt agcgttcgac
      841 gatggtgcgg ctgatccaca gcccgagccc ggtgccgtct ttcttgcgcg tgacgaaggg
      901 tttgaacagc tcggcctgca gttcggggcc gagccccggg ccggtgtcgg cgatctcgat
      961 ctcgaccccg tcgtcggcca cgtcgcgact cgccaggcgc agcgtgccgc cgccttgcat
     1021 cgtgtggatg gcgttgacca gcaggttcac cagcacctgc tgcagttcgt tgcggttgat
     1081 cgccggccgg cgcgtggcgc gcaggtcgcg ctcgatgtgg atgtgcgtct tggcgagcag
     1141 gtgaccgacc agcacgaggc agccctcgag ctcggcctcg gtgtcgacgt tgtcgatgta
     1201 gccggcgtat tcgtcgggcc gcgcgaactg cagcagctgc gtcacgatca ggcgcatgcg
     1261 ctcgatctgc tcgtcgacga gcgcgagttc gctgcgcacc gccttcgccg cctccggccc
     1321 gatgaggtcg cgcaccaggt cgaggttgcc ctggatcacc gcgatcgggt tgttgacttc
     1381 gtgcgcgatg ctggcggtga gctggccgac ggccgccagc ttctcgctgc gcaccagttg
     1441 ctgctgcgcg atctcgagat cgcgcgtgcg ctcgacgacc ttggcgtcga gctcggcgtt
     1501 ccagcgctgc agcgcatggg tgttctcgtc gaccacgtcg agcaggtggt cgaggtggcc
     1561 ggccagttgc ccgatctcgt cgcggttggc aagctcgccg acacgcgcgt cgggcgcacc
     1621 cgactccacg cgccgcatgg tctgcgccat cagctcgatc ggccggaaga tgctgcgcgc
     1681 gccgcgcaac gacacgttgg ccgacaccag catcacggtg aagaagatga gaccgatcgc
     1741 cgccagcatg ccgtacttca gcagcgtgaa cgggcgctcg agaaagccga cgtacagcat
     1801 gccgacgcgc tgcccggcgc cgtcgagcag gggctggtag gccgacacgt accagttgtc
     1861 gacgacgaag gcactgtcga gccaggtgtc gccgtggccg agcacggcgt cgcgcaccgc
     1921 ctgcgagacg cgcgtgccga tcgcgcggtc gttggactcg ctgccgaaca ggcgcacgtt
     1981 ggtgctgatg cgcacatcgt cgaggaacag cgtcgcggtg ccccggctgc cgaacggcaa
     2041 ggcgccttcg gggtagacga tctcgttgat gtggtcgatg aacggcaggt tgcggttcag
     2101 cagtaccccg gcctgcacat ggccgagcag gtggccttcg ccgtcgcgca ccggggcggt
     2161 ggccagcacg accatcgccc gatcttcggc cgcgcgcgtc gtcggcgcgg cgttgcgcgt
     2221 ggccaccagc ggcacgctca ggcgcgcacc cagcgccggc gccaccccga cgagttcacc
     2281 gggctgaaga atctcgatgc tggcgctcgg ccggccgtgc gctgcggcgc gcaaggcggg
     2341 cgggcgctgc ccggcgtcgg ccggcgcgcc ggcgtcggtg acgagcagcg cgccgtcggt
     2401 gtcgcgcagg ttgatgaagt ccagcccttc gcgcgccttg acatcgcgca gcagtgccac
     2461 gagctcggcc gccggccgcg ccgccagcgc cacgtgcagc gcatgcgaat cggccaccgc
     2521 gccggtgctg gcgcccacct cgaccagcac gcgatcgaag tagccgcgcg ccaccgcgag
     2581 atcggaccgt accttggtga tgagcagccg atcgaacgca acattggccc aggcgagcaa
     2641 cagcagaccc agcaacggca gcaccagcgc caa
//
LOCUS       DASRDG010000545         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1689896, whole genome shotgun sequence.
ACCESSION   DASRDG010000545 DASRDG010000000
VERSION     DASRDG010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689896"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /gene="dapB"
                     /locus_tag="VFA35_09140"
     CDS             complement(<1..783)
                     /gene="dapB"
                     /locus_tag="VFA35_09140"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZT56378.1"
                     /translation="MSEPIRVAVAGASGRMGRMLIEAVDAADDLQLAGALDVAGSPAI
                     GQGAAAFLGRTGSPPITADLRTGLANAQVLIDFTRPQGTLAHLAVCRELGVSAVIGTT
                     GFSEAQKAEIAAHAQHIAIMLAPNMSVGVNVLFKLLDLAARALNEGYDIEIIEAHHRH
                     KVDAPSGTALKMGEIVAAALGRDLKDCAVYGREGVTGERDPSTIGFATVRGGDIVGDH
                     TVLFAGTGERIEITHRSGSRVGYAQGALRAARFLTGRSAGLFG"
     gene            complement(780..1259)
                     /locus_tag="VFA35_09145"
     CDS             complement(780..1259)
                     /locus_tag="VFA35_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HZT56379.1"
                     /translation="MPKTDWSTDRFLGVITPYKIEVVQGNVITSEQAAAIKPGMNRAQ
                     VRDVLGSSLLADVFHADRWDYVFTIRRPGTDVQARRVVVLFDGDNFKSMDTGGALPSE
                     REFVASIDTFKTSRNAPSLEMTPEQLKALPAPAKPAAAASAEPIGPTRAYPPLEPNS"
     gene            1459..1887
                     /gene="fur"
                     /locus_tag="VFA35_09150"
     CDS             1459..1887
                     /gene="fur"
                     /locus_tag="VFA35_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HZT56380.1"
                     /translation="MTHADELKNSGLKATLPRLKVLEVFQRTTQRHMSAEDVFKMLLA
                     EGADVGLATVYRVLMQFEQAGILSRNHFETGKAVFELNEGKHHDHLVCIDCGRVEEFF
                     DAEIEKRQRAVAQSRGFELQEHALALYATCTKKNCPHKGK"
     gene            complement(1900..2850)
                     /gene="hprK"
                     /locus_tag="VFA35_09155"
     CDS             complement(1900..2850)
                     /gene="hprK"
                     /locus_tag="VFA35_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HZT56381.1"
                     /translation="MKPTVISAEALFEEHRGILRWEWIAGHAHPERRFDEAAVRNAQS
                     AADLVGYLNYIHPYRVQIVGRREVAYLSQSSPEVTERRISRIVTLEPPVLIVADGETA
                     PDKLVAMCDRADIPLFATGESAGHVIDVVRAYLGLHFAERTTRHGVFMDILGVGVLIT
                     GESGLGKSELGLELISRGHGLVADDAVDLYRVSQTGLEGRCPELLLNLLEVRGIGLLD
                     IKAIFGETAVRRKMRLKLIVHLVRQATMDRDFERLPYEPLNEDILDVPVRKTVIAVDA
                     GRNIAVLVEAAVRNAILQLRGIDTYKEFIERHARAMLKPE"
BASE COUNT          480 a         1048 c         1073 g          471 t
ORIGIN      
        1 gccgaacagc cccgcgctgc ggccggtgag aaagcgcgcg gcgcgcagcg cgccctgcgc
       61 atagccgacg cggctgcccg agcggtgcgt gatctcgatg cgctcgccgg tgccggcgaa
      121 cagcaccgtg tggtcgccca cgatgtcgcc gccgcgcacg gtcgcgaagc cgatcgtcga
      181 cgggtcgcgc tcgccggtca cgccctcgcg accgtagacc gcgcagtcct tgaggtcgcg
      241 gccgagcgcc gccgcgacga tctcgcccat cttcagcgcg gtgccgctgg gtgcgtcgac
      301 cttgtgccgg tggtgcgcct cgatgatctc gatgtcgtag ccttcgttca gcgcgcgcgc
      361 ggccaggtcg agcagcttga acaggacgtt gacgccgacg ctcatgttcg gcgccagcat
      421 gatcgcgatg tgctgcgcgt gcgcggcgat ctcggccttc tgcgcctcgc tgaagccggt
      481 cgtgccgatg accgcgctga cgccgagttc acggcagacg gccaggtgcg cgagcgtgcc
      541 ctgcgggcgc gtgaagtcga tcagcacctg cgcgttggcc aggccggtgc gcaggtcggc
      601 ggtaatcggc gggctcccgg tgcggcccag aaaggcggcg gcgccctggc cgatcgcggg
      661 gctgccggcc acatcgagcg caccggcgag ctgcaggtcg tcggcggcgt cgaccgcctc
      721 gatcagcatg cggcccatgc gcccggaagc gcccgccacc gcgacacgga tcggctcgct
      781 catgaattgg gttcgagcgg cgggtaggcg cgcgtcgggc cgatcggctc ggcgctcgcg
      841 gcagcggccg gcttggccgg cgccggcaag gccttcagct gctccggcgt catctcgagc
      901 gacggcgcgt tgcgcgaagt tttgaacgtg tcgatcgacg cgacgaactc gcgctcggag
      961 ggcagggcgc cgccggtgtc catgctcttg aagttgtcgc cgtcgaacag cacgacgacg
     1021 cgccgcgcct gcacgtcggt gcccgggcgg cggatcgtga agacgtagtc ccagcggtcg
     1081 gcgtggaaca catcggccag cagcgacgag ccgagcacgt cgcgcacctg ggcgcggttc
     1141 atgccgggct tgatcgcggc ggcctgctcg ctcgtgatca cgttgccctg caccacctcg
     1201 atcttgtacg gcgtgatgac gccgagaaag cggtcggtcg accagtcggt cttgggcatg
     1261 gacggcaggt attggcagcc gcccaggccg gcgaccccga tcagccagag cgcacgaaaa
     1321 aacgatgaca cggacatggt acggacgcgg cacgccggaa acgggtgccg acctcgatat
     1381 gatgggtcga ttctagcggc tggcccctgc gggccggccg tcgcttagcc cgacgcccca
     1441 ccgatgaacc cgcccaccat gacgcacgcc gacgaactga agaacagcgg cctgaaggcc
     1501 acgctgccgc gcctcaaggt gctcgaggtg ttccagagga caacgcagcg ccacatgagc
     1561 gccgaagacg tgttcaagat gctgcttgcc gaaggcgccg acgtcgggct ggcgacggtg
     1621 taccgcgtgc tgatgcagtt cgagcaggcc ggcatcctgt cgcgcaacca cttcgagacc
     1681 ggcaaggcgg tgttcgagct caacgagggc aagcaccacg accacctcgt ctgcatcgac
     1741 tgcggccggg tcgaggaatt tttcgatgcc gagatcgaga aacgccagcg cgccgtcgcg
     1801 cagtcgcgcg ggttcgagct gcaggagcat gcgctggccc tgtacgccac ctgcaccaag
     1861 aagaactgcc cgcacaaagg gaagtaaccg ggggcatccc tattctggtt tgagcatcgc
     1921 cctggcgtgg cgctcgatga actccttgta ggtgtcgatc ccgcgcagtt ggagaatcgc
     1981 gttgcgcacc gccgcctcga ccagcaccgc gatgttgcgc ccggcgtcga ccgcgatcac
     2041 ggtcttgcgc accggcacgt cgaggatgtc ctcgttcagc ggctcgtagg gcaggcgctc
     2101 gaagtcgcgg tccatggtcg cctggcgcac caggtggacg atcagcttca gccgcatctt
     2161 ccgccgcacc gcggtctcgc cgaagatcgc cttgatgtcg agcaggccga tgccgcgcac
     2221 ctcgagcagg ttcagcagca gttcggggca gcggccttcg aggccggtct gcgacacgcg
     2281 gtacagatcg accgcgtcgt cggcgaccag cccgtggccg cgcgagatca actcgagccc
     2341 gagttcgctc ttgcccaggc ccgactcgcc ggtgatcagc acgcccacgc cgaggatgtc
     2401 catgaacacg ccgtggcgcg tcgtgcgctc ggcaaagtgc aggccgaggt aggcccgcac
     2461 cacgtcgatc acgtggccgg ccgattcacc ggtggcgaac agcgggatgt cggcgcgatc
     2521 gcacatcgcg accagcttgt cgggggcggt ctcgccgtcg gccacgatca gcaccggcgg
     2581 ctcgagcgtc acgatgcgcg aaatgcggcg ctcggtgact tcgggcgagc tctggctcag
     2641 ataggcgacc tcgcggcggc cgacgatctg cacccggtac ggatggatgt agttcagata
     2701 gccgaccaga tcggctgccg actgcgcgtt gcggaccgcc gcttcatcga atcggcgttc
     2761 ggggtgcgca tgcccggcga tccactccca gcgaaggatg ccgcggtgct cctcaaaaag
     2821 ggcctccgcg ctgatgacgg tcggtttcat gaggcccctc gcccgtcggg tacgccggac
     2881 gttcccccaa ggggactcgg gccggcttgg gagcggcccg gcgctcggcc cgagccgcta
     2941 cgggccacca tggcagacat tcgctaggcc accgacttca gaggctccca gttcgcgatc
     3001 agctggtgca cggtggccgc gtcgggctcg gtcttgaggc gctcgcgcag ttcccggtcg
     3061 gacagcatct cg
//
LOCUS       DASRDG010000546         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1689993, whole genome shotgun sequence.
ACCESSION   DASRDG010000546 DASRDG010000000
VERSION     DASRDG010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689993"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /gene="uppS"
                     /locus_tag="VFA35_09160"
     CDS             complement(<1..592)
                     /gene="uppS"
                     /locus_tag="VFA35_09160"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZT56382.1"
                     /translation="MKREGRPSELAVPRSVAVIMDGNGRWARQAGLERIKGHEKGTDA
                     VRTTVTECARLGVQALTLYAFSEENWQRPPREVELLMKLLDRFLVQERPTLMDNRVRL
                     VHAGRRERLRPRTLALLDETIALTKDNAGMVLCLALSYGGRQELVDAVRALARDVAAG
                     RLAADAVDEAAVTARLYQPALPDPDLLIRTAGELRIS"
     gene            complement(589..1860)
                     /locus_tag="VFA35_09165"
     CDS             complement(589..1860)
                     /locus_tag="VFA35_09165"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZT56383.1"
                     /translation="MATTCVVGILWGDEGKGKVIDFLAAEADFVVRFGGGHNAGHTLI
                     LPSGKCVLHLVPSGIVHPHVVNVVGNGVVVDPLHLLGEIARLRERGLRIEPGRNLLVS
                     ERAHVILEAHRTQDQWHEQARGKGRIGTTGRGIGPCYADRCARIGLRVGDLLRSERLR
                     DGLERLCAEKNPMFTAVGLRALDPAALYETLAPAGEQLRAAVVDAGAVLRAAHRDGRR
                     VLLEGAQGCLLDVEHGTYPFVTSSHASTGGAFSGTGLPPHAMRVIGVAKAYATRVGEG
                     PFPSELRGELGDKLRVAGNEFGSTTGRPRRCGWFDAVAVRYAGELSGAQELTLTNLDV
                     LRGFDPLQIATAYQQPGGAATRAFPAFDLEAAAPVFATLPGFHEDVRAVRRFDDLPAA
                     ARRYVEAIERETGLPVKTISVGPERQQVILR"
     gene            1919..>2646
                     /locus_tag="VFA35_09170"
     CDS             1919..>2646
                     /locus_tag="VFA35_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56384.1"
                     /translation="MTESPAPGQQPEGCASAETALALFETFARRLLPGALRRLASRRG
                     YTDARRLDLLADVKQELRLDCVEHAGLIAQLSAPERHQRWFRLAERWVYRQRLRVDRV
                     GEARHAREPSASTPPPDAAALAPTLADVLPGLSPRLRDALARAARQHRNGRANVAATA
                     APLGLHPRRVRQLWEEAAARLGYGDEYLQFWRQRLAEALTGLAADLLRDRGQCFLLAG
                     PRNRPDPAGRLRRIRRIRSQLMVRP"
BASE COUNT          328 a         1009 c          985 g          324 t
ORIGIN      
        1 tgctgatgcg cagctccccc gcggtgcgga tcagcagatc gggatcgggg agcgcgggct
       61 ggtagaggcg cgcggtgacg gccgcttcgt cgaccgcgtc cgcggccagc ctgccggcgg
      121 cgacgtcgcg cgccagcgcg cgcacggcgt cgaccagctc ctggcggccg ccgtacgaga
      181 gcgcgaggca cagcaccatg ccggcgttgt ccttcgtcag cgcgatcgtc tcgtcgagca
      241 gcgcgagcgt gcgcggccgc aggcgctcgc ggcgtcccgc gtgcacgagc cgcacgcggt
      301 tgtccatcag cgtcggccgc tcctgcacga ggaagcggtc gagcagcttc atcagcagct
      361 cgacctcgcg cggcggccgc tgccagttct cctccgagaa cgcgtagagc gtgagcgcct
      421 gcacgccgag ccgcgcgcac tcggtgaccg tcgtgcgcac cgcgtccgtg cccttctcgt
      481 ggcccttgat gcgctcgagt cccgcctgcc tcgcccagcg gccgttgccg tccatgatca
      541 ccgcgaccga gcgcggcacc gcgagctccg acgggcgtcc ttcgcgcttc accgcaggat
      601 gacctgctgg cgctccggcc ccacggagat cgtcttcacc ggcaggccgg tctcgcgctc
      661 gatcgcctcg acgtagcggc gcgcggcggc gggcaggtcg tcgaaccgcc gcacggcgcg
      721 cacgtcctcg tggaagccgg gcagcgtcgc gaacaccggc gccgccgcct cgaggtcgaa
      781 cgcggggaac gcgcgcgtcg cggcgccgcc gggctgctgg taggcggtgg cgatctgcag
      841 cgggtcgaac ccgcgcagca cgtcgaggtt ggtcaacgtg agctcctgcg cgcccgacag
      901 ctcgccggcg tagcgcacgg cgacggcgtc gaaccagccg cagcggcgcg gccggcccgt
      961 ggtcgagccg aactcgttgc ccgcgacgcg cagcttgtcg ccgagctcgc cgcgcagctc
     1021 gctcgggaac ggcccttcgc cgacgcgcgt cgcgtaggcc ttggcgacgc cgatcacgcg
     1081 catggcgtgc ggcggcaggc cggtgcccga gaacgcgccg ccggtggacg cgtgcgagct
     1141 ggtgacgaac ggatacgtgc cgtgctcgac gtcgagcagg cagccctgcg cgccctcgag
     1201 cagcacgcgc cggccgtcgc ggtgcgcggc ccgcagcacg gcgccggcgt cgacgacggc
     1261 ggcgcgcagc tgttcgccgg cgggcgcgag cgtctcgtac agcgccgcgg ggtcgagcgc
     1321 gcgcaggccg acggccgtga acatcgggtt cttctcggcg cacaggcgct cgagcccgtc
     1381 gcgcaggcgc tcggaccgca gcaggtcgcc gacacggagc ccgatgcgcg cgcagcggtc
     1441 ggcgtagcac gggccgatgc cgcggcccgt ggtgccgatc cggcccttgc cgcgcgcctg
     1501 ctcgtgccac tggtcctgcg tgcggtgcgc ctcgaggatc acgtgcgcac gctcgctcac
     1561 caggaggttg cggcccggct cgatgcgcag gccgcgttcg cgcagccggg cgatctcgcc
     1621 gagcaggtgc agcgggtcga cgacgacgcc gttgccgacc acgttgacga cgtgcgggtg
     1681 cacgatgccc gacggcacca ggtgcagcac gcacttgccc gacggcagga tgagcgtgtg
     1741 cccggcgttg tggccgccgc cgaaccgcac cacgaagtcg gcctccgcgg cgaggaagtc
     1801 gatgaccttc cccttccctt cgtcacccca gaggatgcca acgacacagg tggtggccat
     1861 gaatgcggct gcggaaaaag ggcggcagca tagcaccgcc ggccgcgagg agcacgcgat
     1921 gaccgagtcc cccgcgccgg gtcaacagcc ggaaggttgc gcgagcgccg agaccgcgct
     1981 cgcgctgttc gagacgttcg cccgccgcct gctgcccggc gccctgcggc gactggccag
     2041 ccggcgcggc tacaccgatg cgcggcggct cgacctgctc gccgacgtga agcaggagct
     2101 gcggctcgac tgcgtcgagc acgccgggct gatcgcgcag ctgtcggcgc cggagcgcca
     2161 ccagcgctgg ttccggctcg ccgagcggtg ggtctaccgg cagcggctgc gcgtcgaccg
     2221 cgtgggcgag gcgcggcacg cgcgcgaacc gagcgccagc acgccgccgc ccgacgccgc
     2281 ggcgctcgcg ccgacgctcg ccgacgtgct gcctgggctg tcgccgcggc tgcgcgacgc
     2341 gctggcccgc gccgcccgcc agcaccgcaa cggccgcgcg aacgtcgccg ccaccgcggc
     2401 gccgctcggc ctgcatccgc gccgcgtgcg gcagctgtgg gaggaggcgg cggcgcggct
     2461 cggctacggc gacgagtacc tgcagttctg gcgccagcgc ctcgccgagg cgctgacggg
     2521 cctcgccgcc gacctgctgc gcgaccgcgg ccagtgcttc ctgctcgcgg gcccgcgcaa
     2581 ccgacccgat cccgccggcc gtctgcggcg gatccgccgg atccgcagcc agctcatggt
     2641 gcggcc
//
LOCUS       DASRDG010000547         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1690148, whole genome shotgun sequence.
ACCESSION   DASRDG010000547 DASRDG010000000
VERSION     DASRDG010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690148"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1125)
                     /locus_tag="VFA35_09175"
     CDS             complement(73..1125)
                     /locus_tag="VFA35_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZT56385.1"
                     /translation="MTPEVALILGIDGLANGAVYLLAGLGLVLIFSVTRVVFVPFGDV
                     AAFAALSLAAFETGRMPATVGMVAALAALALAVEIRSLVVRHESRRIPRALVGWGLLP
                     ALPCFAAWAIAGRPVPAMLQVAVAVLLVVPIIPLVARIALQPIADASVLVLLIASLAL
                     HFLLSGLGLLFFGPEGSRTAPLVGGALSLGGFGISHQVLLMVGAAVVLSALFFLAFER
                     TIAGKALRATAVNRIGARLVGIRPARTALLAYICASLLAGLIGVLVAPVTTMYYDSGF
                     LIGLKAFVAAIIGGLVSYPLTALGAIAVGVVESFASFWSGALKDVIVFSLLIPVLLAR
                     SFFSVHAEEEEEEIDE"
     gene            complement(1188..>2132)
                     /locus_tag="VFA35_09180"
     CDS             complement(1188..>2132)
                     /locus_tag="VFA35_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT56386.1"
                     /translation="DASDPSTAARNARKMIDEDKVDVIIGTAGSPGALAIAAVARETK
                     TPLISIANANLPGDDGAWMVTLPQPAPLMVSAVVERMKKSGVKTVGYIGFSDAWGDLV
                     YDALLKATEPAGIKVVANERYARPDPSVTGQVLKIVALRPDAVMTGGAGTPGALPFLA
                     LQERGFKGGIYGNHGMINPDFLRVAGSAATGAFLPTGPVIVADQLPANYPTRKMTDDF
                     RAAFLKVNGTPSTDAFSAYSFDGYLVFADAAARAIATKAEPGTPAFRVALRDAIATTK
                     EVVGTHGVYNFKAGNVYGVDERARVVVKVDNGTWKYVP"
BASE COUNT          350 a          738 c          778 g          266 t
ORIGIN      
        1 cgagcgccag cggcgcgagc acgatcgcgg cgacgagcac cgcaaccgcg gcgcgatgcc
       61 tgccctgggc cgtcattcgt cgatctcctc ttcctcctcc tcggcgtgga ccgagaagaa
      121 ggaccttgca agcagcaccg ggatcaagag gctgaagacg atcacgtcct tgagcgcgcc
      181 gctccagaac gaggcaaaac tctcgaccac gccgaccgcg atcgcgccga gcgcggtgag
      241 cgggtagctg accaggccgc cgatgatcgc ggcgacgaac gccttcaggc cgatcaggaa
      301 gccggagtcg tagtacatcg tcgtcaccgg cgccaccagc acgccgatca agccggcgag
      361 cagcgacgcg cagatgtagg cgagcagcgc ggtgcgcgcc gggcggatgc cgaccaggcg
      421 cgcgccgatg cggttgacgg cggtggcgcg caacgccttg ccggcgatgg tgcgctcgaa
      481 ggcaaggaag aagagggcgc tcagcaccac cgcggcgccg accatgagca gcacctggtg
      541 gctgatgccg aagccgccga gggagagcgc gccgccgacc agcggcgcgg tgcgcgagcc
      601 ctcggggccg aagaacagca gccccaggcc ggagagcagg aagtgcagcg ccagcgaggc
      661 gatcagcagc accagcaccg aggcgtcggc gatcggttgc agggcgatgc gcgccaccag
      721 cggaatgatc ggcacgacca gcagcaccgc gaccgcgacc tgcagcatcg ccggcaccgg
      781 ccggccggcg atcgcccagg cggcgaagca gggcagcgcc ggcagcaggc cccagccgac
      841 gagcgcccgc gggatgcgcc gggactcgtg gcgcacgacc aggctgcgaa tctcgaccgc
      901 cagcgcgagc gcggccagcg ccgccaccat ccccaccgtc gccggcatgc ggccggtctc
      961 gaacgccgcc aggctgagcg cggcgaacgc ggcgacgtcg ccgaacggga cgaagacgac
     1021 ccgcgtcacc gagaagatca gcaccagccc cagcccggcg agcaggtaga ccgcgccgtt
     1081 ggcgaggccg tcgatgccga ggatgagggc gacctcgggc gtcatgccgg agcgggactg
     1141 cgaatcggtg cgcggctctt cgtcttccgg atcagctcat cgccgcgtca gggcacgtat
     1201 ttccaagtgc cgttgtcgac cttcaccacc acccgcgccc gctcgtcgac gccgtagacg
     1261 ttgccggcct tgaagttgta gacgccgtgg gtgccgacca cttccttggt ggtggcgatg
     1321 gcgtcgcgca gggcgacgcg aaacgccggc gtgcccggct cggccttggt cgcgatcgcc
     1381 cgcgccgccg cgtcggcgaa caccaggtag ccgtcgaacg aataggcgga gaaggcgtcg
     1441 gtcgagggcg tgccgttgac cttgaggaag gcggcgcgga agtcgtcggt catcttgcgc
     1501 gtcgggtagt tcgcgggcag ttggtcggcg acgatgaccg ggccggtcgg caggaacgcg
     1561 ccggtcgcgg ccgagccggc gacgcgcagg aagtcgggat tgatcatccc gtggttgccg
     1621 tagatgccgc ccttgaagcc gcgctcctgc agcgccagga agggcagtgc gccgggcgtg
     1681 ccggcgccgc cggtcatcac cgcgtcgggc cgcagcgcca cgatcttcag cacctggccg
     1741 gtgaccgacg ggtcggggcg ggcatagcgc tcgttggcca cgaccttgat gccggccggc
     1801 tcggtcgcct tcagcaaggc gtcgtagacc aggtcgcccc aggcgtcgga gaagccgatg
     1861 tagcccaccg tcttgacgcc cgacttcttc atgcgctcga ccaccgcact gaccatcagc
     1921 ggcgcgggtt gcggcagcgt gaccatccag gcgccgtcgt cgccgggcag gttggcgttg
     1981 gcgatcgaga tcagcggcgt cttcgtctcg cgcgccaccg cggcgatcgc cagcgcaccg
     2041 ggcgagccgg cggtgccgat gatgacgtcg accttgtcct cgtcgatcat cttgcgcgcg
     2101 ttgcgggcgg cggtcgacgg gtccgaggcg tc
//
LOCUS       DASRDG010000548         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1690604, whole genome shotgun sequence.
ACCESSION   DASRDG010000548 DASRDG010000000
VERSION     DASRDG010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690604"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFA35_09185"
     CDS             complement(<1..428)
                     /locus_tag="VFA35_09185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT56387.1"
                     /translation="MTDIIVEPLGRQHDRTSFQCGAEALDRYLKQQARQDADKRVAAP
                     FVAVRPRHTRVLGYYTLSASVLTLADLPDDLARKLPRYPQLPVTLLGRLAVDQSTKGQ
                     GLGEHLLLDALRRSLAHADHIAAMAVVVDAKDEAAAAFYR"
     gene            complement(438..731)
                     /locus_tag="VFA35_09190"
     CDS             complement(438..731)
                     /locus_tag="VFA35_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HZT56388.1"
                     /translation="MANQDSPDTTRDARLEARVSAAQKHLLQQAAALSGRTLSEFVVA
                     SAQDAARRVIAEHESIRLSREEQLVFVQALLKPLEPNARLKRAAKAYRQRTGA"
     gene            complement(833..1837)
                     /locus_tag="VFA35_09195"
     CDS             complement(833..1837)
                     /locus_tag="VFA35_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT56389.1"
                     /translation="MRKTTLLLSLILAGIATTASAADKVTLQLKWLPQAQFAGYYVAA
                     AKGFYKEADLDVTIKPGGPDIAPAQVLAAKGADLTVDWMPSALAAREAGVPMVNVAQI
                     FNRSGMMLTCKKASGVTTSKDFKGKTLGVWFGGNEYPFLSWMGTLGLKTSGANPDVKV
                     LKQGFNVDPLLQNQAACISTMIYNEYWQVIDAGVKPADLVTFPYEDQGVATLEDGLYA
                     REPDLKDPAYVAKIAKFVKASIKGWEYAIKNQDEAVKIVLAADTSGGSTAAVQKRQLE
                     NVAKLITTAGTPKMGVLDPAAYQRTVKVLLAGGSDPVIKKDPGAAAWTHAVTDKALAK
                     "
BASE COUNT          303 a          649 c          695 g          355 t
ORIGIN      
        1 tgccgataga acgcggcggc ggcctcgtcc ttggcatcga caaccaccgc catcgcggcg
       61 atgtggtccg catgggccaa gctgcggcgc agggcatcca gaagcagatg ttccccgagg
      121 ccttggcctt tggtcgattg atcgacggcc agccggccca gcagcgtgac gggaagctgc
      181 ggatagcgcg gcagcttgcg cgccaagtca tccggcagat cggccagggt cagcaccgag
      241 gccgacagcg tgtagtagcc gagtacgcgc gtgtggcgcg gcctaacggc gacgaaaggc
      301 gcggccacgc gcttgtccgc gtcctgccgg gcctgctgct tgagatagcg gtcgagcgcc
      361 tccgcgccgc actggaagga ggttcgatcg tgctgtcggc ccagaggctc gacgatgatg
      421 tctgtcaaga ccaccggcta tgcgccggtg cgttggcggt aggccttggc tgcgcgcttg
      481 aggcgcgcgt tcggctccag cggcttcagc agggcttgaa caaacaccaa ctgctcctct
      541 cgcgacaggc ggatcgactc gtgctcggca atcacccggc gtgctgcgtc ctgcgcactg
      601 gcgacgacaa actcgctcag cgtgcggccg gaaagcgcgg ccgcttgctg gagcaggtgc
      661 ttctgcgccg cggacacgcg agcttcgagc cgggcgtcac gggtggtgtc ggggctgtct
      721 tgattggcca tgatcgtctt cctgatcaaa tgtacggcaa gtgtccgtac gcggcaagcg
      781 acgccgcagc cgcggccgcg gcggtcgaat cgcaacccgc tgcaagcacc gcttacttcg
      841 cgagcgcctt gtcggtgacc gcgtgcgtcc acgcggcggc gcctgggtct ttcttgatca
      901 ccgggtcgct gccgccggcg agcagcacct tgaccgtgcg ctggtacgcg gccgggtcga
      961 gcacgcccat cttcggcgtg ccggcggtcg tgatcagctt ggccacattc tcgagctggc
     1021 gcttctgcac cgcggcggtc gagccgcccg aggtgtcggc ggcgagcacg atcttgacgg
     1081 cttcgtcctg gttcttgatc gcgtattccc aacccttgat cgaggccttg acgaacttcg
     1141 cgatcttcgc gacataggcc gggtccttca ggtcgggctc gcgcgcgtac aggccgtctt
     1201 cgagcgtcgc gacgccctgg tcttcgtacg ggaaggtcac gaggtcggcc ggcttcacgc
     1261 cggcgtcgat cacctgccag tactcgttgt agatcatcgt cgagatgcac gcggcctggt
     1321 tctgcagcag cgggtcgacg ttgaagcctt gcttgagcac cttcacgtca gggttcgcgc
     1381 cgctggtctt caggccgagc gtgcccatcc acgacaggaa cgggtactcg ttgccgccga
     1441 accagacgcc gagcgtcttg cccttgaagt ccttggaggt ggtgacgccg ctggccttct
     1501 tgcaggtcag catcatcccg gagcggttga agatctgcgc gacgttgacc atcggcacgc
     1561 cggcctcgcg cgcggcgagt gcgctcggca tccagtcgac cgtcaggtcg gcgcccttgg
     1621 cggcgagcac ctgcgccggc gcgatgtcgg ggccgcccgg cttgatcgtc acgtcgaggt
     1681 cggcctcctt gtagaagccc ttcgcggcgg cgacgtagta gccggcgaac tgcgcctgcg
     1741 gcagccactt gagctgcagc gtgaccttgt cggccgcgct ggccgtggtg gcgatgccgg
     1801 ccaggatcaa agagagcagg agagtcgttt tgcgcatcga tgaactccga ggaggttgtg
     1861 ccttgcggcg tgggggaagc aggatgggaa ttcaagcatg ggcgcggatc gacggatgcc
     1921 agaaggtcgc gcgccgctcg acccagccca gcagcccata cagcagcgag ccggcgattg
     1981 ccgcgaccag gatcgcggcc ca
//
LOCUS       DASRDG010000549         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1596902, whole genome shotgun sequence.
ACCESSION   DASRDG010000549 DASRDG010000000
VERSION     DASRDG010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596902"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFA35_09200"
     CDS             <1..588
                     /locus_tag="VFA35_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZT56390.1"
                     /translation="MALKAEQRRAEVARLEALKKKVEDVLAANPKLAALSPQIRLEMT
                     RDGLRIQIVDAQNRPMFDSGSAVVKPYMRELLHEIGAMLGEVPNRLTLEGHTDAQPFG
                     SGERGYSNWELSSDRANASRRELGAGGLPEERVLLVQGLASSMPFDAKDPAGAANRRI
                     SILVMNRDAEDRLLRLKPDDEEAAASPSIPAPITR"
     gene            635..1033
                     /locus_tag="VFA35_09205"
     CDS             635..1033
                     /locus_tag="VFA35_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZT56391.1"
                     /translation="MSHPTEMKFLIVDDFSTMRRIVRGLLKEIGYNNADEAEDGSVAL
                     TMLKNAKYDFVVSDINMPVMNGFELLAAVKADDSLKHLPVLMVTAEARKEDIVRAAKD
                     GAAGYVVKPFTKATLEEKVQKIMQKLAATA"
     gene            1064..1696
                     /locus_tag="VFA35_09210"
     CDS             1064..1696
                     /locus_tag="VFA35_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HZT56392.1"
                     /translation="MKMDDLDLLAPQMAAGASAEVFQQLGLITRQLHDTLTQLGVMPR
                     LQSAAEGLPDARSRLSYIAKKTGDAANKVLNSVDMAKAEHHSISAETRRIADAILKDP
                     VKAVATGAVLNFVGDVEAATERIDRHLTDIMMAQDFHDLTGQVVAKVVALAADLEDSL
                     VKLLVQAAPPEQAQRVESSILNGPVVNPEGRTDVVANQGEVDDLLASLGF"
     gene            1846..3009
                     /locus_tag="VFA35_09215"
     CDS             1846..3009
                     /locus_tag="VFA35_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HZT56393.1"
                     /translation="MSGPSAQDRNLPASERKLRKAREEGQVARSRDFGHFAAIAVCGA
                     LLVSAAPLLAEWLQQTLMQGLHFDRAAILNPDAMGERLLALTIRLMWVIVPFGLAMMA
                     TALLGSLLIGGWNWTLKPLLPNFGKLNPITGLPGIVTKDKLIDALKGSALALILGAIG
                     ALYLKAHVDELAGVLALPLPAAIRQALGAMGGGLGLLLLALAAFALIDVPLQRFQHAQ
                     RLKMSHQELKQEHKELEGNQEIKAKLRARMREATRRRMMAAVPQADLVVMNPTHYAVA
                     LKYDDKKMGAPRVVAKGADLLALKIRDLAKDSQVPVLQAPVLARALYAHAELDREIPA
                     ALFAAVAQVLAYVYQLRAALAGQVAMPNALPELNVPKELDPQHTPVPGMEVFD"
BASE COUNT          571 a         1112 c         1084 g          424 t
ORIGIN      
        1 atggcgctga aggccgagca gcgacgcgcc gaggtcgcgc gcctcgaggc gctgaagaag
       61 aaggtcgagg acgtgcttgc ggcgaacccg aagctcgccg cactcagccc ccagatccgg
      121 ctcgagatga cgcgcgacgg gctgcgcatc cagatcgtcg acgcgcagaa ccggccgatg
      181 ttcgacagcg gcagcgcggt cgtcaaaccg tacatgcgcg agctgctaca cgagatcggc
      241 gcgatgctcg gcgaggtgcc gaaccgcctg acgctcgaag gccacaccga cgcccagccc
      301 ttcggcagcg gcgagcgcgg ctacagcaac tgggagctgt cgagcgaccg cgcgaacgcc
      361 tcgcgccgcg agctcggcgc gggcggcctg cccgaagagc gcgtgctgct ggtgcagggg
      421 ctggcatcga gcatgccgtt cgatgcgaaa gacccggccg gcgcggcgaa ccgccgcatc
      481 agcatcctcg tgatgaaccg cgatgccgaa gaccgcctgc tgcggctgaa gcccgacgac
      541 gaagaagccg ccgcgtcgcc ctcaattcca gccccgatca cccgataaag acgccatgcc
      601 cggccgtgtg ccggagtgac ccgcgaggag cgccatgagc caccccactg agatgaaatt
      661 cctgatcgtc gacgacttct cgaccatgcg ccgcatcgtg cgcggcctgc tgaaggagat
      721 cggctacaac aacgccgacg aggccgaaga cggatcggtc gcgctgacca tgctgaagaa
      781 cgccaagtac gacttcgtcg tcagcgacat caacatgccg gtgatgaacg gcttcgagct
      841 gctcgccgcc gtcaaggccg acgacagcct caagcacctg ccggtgctga tggtgaccgc
      901 cgaggcgcgc aaggaagaca tcgtgcgcgc cgcgaaagac ggcgccgccg gctacgtcgt
      961 gaagcccttc accaaggcca ccctcgaaga gaaggtgcag aagatcatgc aaaagctcgc
     1021 ggccacggcc tgagcggcac agcagcatcc ggagcagaag aacatgaaga tggatgacct
     1081 ggacctgctc gcgccgcaga tggccgcggg cgcctcggcc gaggtgtttc agcaactcgg
     1141 cctgatcacg cgccagctgc acgacacgct cacgcaactc ggcgtgatgc cgcgcctgca
     1201 gtcggccgcc gagggcctgc ccgacgcacg cagccgcctg agctacatcg cgaagaagac
     1261 cggcgacgcc gcgaacaagg tgctgaactc ggtcgacatg gccaaggccg agcaccacag
     1321 catcagcgcc gagacgcgcc gcatcgccga cgcgatcctg aaggacccgg tcaaggcggt
     1381 cgcgaccggc gcggtgctga acttcgtcgg cgacgtcgag gccgcgaccg agcgcatcga
     1441 ccgccacctg accgacatca tgatggcgca ggactttcac gacctgaccg gccaggtggt
     1501 cgcgaaggtc gtcgcgctcg ccgccgacct cgaagacagc ctcgtcaagc tgctcgtgca
     1561 ggccgcgccg ccggagcagg cgcagcgcgt cgaatcgtcg atcctgaacg gtccggtcgt
     1621 gaaccccgaa ggccgcaccg acgtggtcgc caaccagggc gaggtcgacg acctgctcgc
     1681 cagcctcggg ttctgagggc gcggcgggcg agcgcagagg cggcccgcgg gccgccgctt
     1741 gcgttgggcg cctgatgcct cggtgcgtgc accgccgcga acagcgcgcg cttcaccccg
     1801 cttatccggc gcaggcggcg cgcccgggcc ggaacaatca cgcggatgtc cggaccgagc
     1861 gcccaagacc gcaacctgcc cgcgtcggag cgcaagctca ggaaggcgcg cgaagaaggc
     1921 caggtcgcgc gctcgcgcga cttcgggcac ttcgcggcga tcgcggtgtg tggcgcgctg
     1981 ctcgtcagcg cggcgccgct gctggccgag tggctgcagc agacgctgat gcagggcctg
     2041 cacttcgacc gcgcggcgat cctgaacccc gacgccatgg gcgagcggct gctcgcgctg
     2101 acgatccggc tgatgtgggt catcgtgccc ttcggcctcg cgatgatggc gaccgcgctg
     2161 ctcggcagcc tcctgatcgg cggctggaac tggacgctga agccgctgct gcccaacttc
     2221 ggcaagctca acccgatcac gggcctgccc ggcatcgtca cgaaagacaa gctgatcgat
     2281 gcgctcaagg gcagcgcgct ggcgctgatc ctcggcgcga tcggcgcgct gtacctgaag
     2341 gcgcatgtcg acgaactcgc cggcgtgctc gcgctgccgc tgcccgcggc gatccggcag
     2401 gcgctcggcg cgatgggcgg tggactcggg ctgctgctgc tcgcgctcgc cgcgttcgcg
     2461 ctgatcgacg tgcccctgca gcgctttcag cacgcgcagc gcctgaagat gagccaccag
     2521 gagctgaagc aggagcacaa ggagctcgaa ggcaaccagg agatcaaggc caagctgcgc
     2581 gcccgcatgc gcgaggcgac ccggcgccgc atgatggccg cggtgccgca ggccgatctt
     2641 gtcgtgatga acccgaccca ctacgcggtc gctctgaagt acgacgacaa gaagatgggc
     2701 gcgccgcgcg tcgtcgcgaa gggcgccgac ctgctggcgt tgaagatccg cgatctcgcg
     2761 aaggactcgc aggtgcccgt gctgcaggcg ccggtgctgg cgcgcgcgct gtacgcgcat
     2821 gccgaactcg accgcgagat ccccgccgcg ctgttcgccg cggtcgcgca ggtgctggcc
     2881 tacgtgtacc agctgcgcgc cgcgctcgcc ggccaggtcg cgatgccgaa tgcgctgccg
     2941 gagttgaacg tgccgaagga actcgacccg cagcacaccc cggtgccggg catggaggtc
     3001 ttcgactgac cggggcgtgg aaccgaagcg cggcagtcgg cttgcaaggc cagggcctac
     3061 cctgaactca cgcccgagga catccgtgcc tgcctggccc aagccgccga gcgcacgccc
     3121 gttggcaatt tgcctacgtc gtagacaatt cttctacaat gtagccattc cacctacgat
     3181 gtggccgttt c
//
LOCUS       DASRDG010000550         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1118043, whole genome shotgun sequence.
ACCESSION   DASRDG010000550 DASRDG010000000
VERSION     DASRDG010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118043"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /gene="flgK"
                     /locus_tag="VFA35_09220"
     CDS             complement(<1..1323)
                     /gene="flgK"
                     /locus_tag="VFA35_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZT56394.1"
                     /translation="MSALLSIGKTAMFANYAALQTVGNNIANANTAGYSRQTTQLADA
                     TGQFTGSGFFGKGVTVTTVARAYDRYLTNQAVGSAAVAAGDAARLDKLTQLESVFPIG
                     SSGVGYAAGEFLNAFVDLSNNPADASARQVVLGQASELASRFRAAGDQLSALQTGVTQ
                     DIKATVGQVNALAQQVAALNAQIAALKGSGQPPNQLLDQRDALVGQIAASINVSTIAA
                     DDGSVGVFIGGGQNLVLGATANTLKAVPDAFDPAKAMLALSEGAVDRVIPPDSLAGGA
                     LAGLLKFQNDDLGAATNLLGQMAAAVSGAVNQQQALGLDLGQPAGPGAPIFAVGAARV
                     LGAAANTGTAALTLTVDDASKLQASDYEMSFDGSAYTLTRLSDQRAVPGGPFTPAQLA
                     AGVQVDGATLRLTGGAANNSDRFLLQPLAVAAQGMQTVLASTQGLAAAA"
     gene            complement(1335..2216)
                     /gene="flgJ"
                     /locus_tag="VFA35_09225"
     CDS             complement(1335..2216)
                     /gene="flgJ"
                     /locus_tag="VFA35_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HZT56395.1"
                     /translation="MAISGYSATTGDAGALDATGSALRARAASDPKAAIRMAAKQFEA
                     MFMQQLMKSMRESSLGTGMLDNAGTQMGTEMLDTQFANKMSGAPGGLADVIARQLERQ
                     MNGVAPQVAAPAAGAAGAPTAKLANVTPNQQEFLRRHTAAAAGAEAETGIPATFMVAQ
                     AAHESGWGRREIRHEDGTSAHNLFGIKAGAGWQGRVAQVMTTEVIDGQPRKVMAKFRA
                     YASDAESFRDYAQLMKNNPRYAGVVAAGNSAQGFAQGLQRAGYATDPAYADKLTRVIN
                     TTLRLQRAAVHAPQSLA"
     gene            complement(2219..>2351)
                     /locus_tag="VFA35_09230"
     CDS             complement(2219..>2351)
                     /locus_tag="VFA35_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HZT56396.1"
                     /translation="APGAKLADVVKALNTLGATPQDLLAILQAMKTAGALNAEIEVI"
BASE COUNT          280 a          832 c          851 g          388 t
ORIGIN      
        1 cgccgccgcg gccaacccct gggtactggc gagcacggtc tgcatgccct gcgccgcgac
       61 cgcgagcggc tgcagcagga agcggtcgct gttgttcgcc gcgccgccgg tcagccgcag
      121 cgtcgcgccg tcgacctgca cgccggccgc gagctgcgcc ggcgtgaacg ggccgccagg
      181 cacggcgcgc tggtccgaca ggcgggtcag cgtgtaggcg ctgccgtcga agctcatctc
      241 gtagtcgctc gcctgcagct tcgaggcgtc gtccacggtc agcgtcaacg cggcggtgcc
      301 ggtgttcgcc gcggcaccga gcacgcgcgc ggcgccgacc gcgaagatcg gtgcgccggg
      361 gccggcgggc tggccgaggt cgaggccgag cgcctgttgt tggttgaccg cgccgctgac
      421 ggccgcggcc atctgaccga gcaggttggt ggcggcgccg aggtcgtcgt tctggaactt
      481 cagcaggccg gcgagcgcac cgccggccag gctgtcgggc gggatcaccc ggtcgaccgc
      541 gccttcgctc agcgccagca tcgccttggc cgggtcgaac gcgtcgggaa cggccttcag
      601 cgtgttcgcg gtcgcgccga gcacgaggtt ctggccgccg ccgatgaaca cgccgacgct
      661 gccgtcgtcg gccgcgatcg tgctcacgtt gatgctcgcg gcgatctgac cgacgagcgc
      721 gtcgcgctgg tcgagcagct ggttcggcgg ctggccgctg cctttgagcg cggcgatctg
      781 cgcgttcagc gcagcgacct gctgcgccaa cgcgttcact tggccgaccg tggccttgat
      841 gtcttgcgtg acgccggtct gcagcgcgct caactgatcg ccggcggcac ggaagcgcga
      901 cgcgagctcg ctcgcctggc ccagcaccac ctgccgcgcc gacgcgtcgg cggggttgtt
      961 cgacaggtcg acgaacgcgt tcaggaactc gcccgccgcg tagccgacgc cgctgctgcc
     1021 gatcgggaac acgctctcca gctgtgtgag cttgtcgagc cgcgccgcgt cgccggccgc
     1081 gaccgcggcg ctgccgacgg cctggttcgt caggtacctg tcgtaggcgc gcgcgaccgt
     1141 cgtgaccgtg acacccttgc cgaagaagcc cgagccggtg aactggcccg tggcgtcggc
     1201 aagctgcgtg gtctggcgcg aatagccggc ggtgttggcg ttcgcgatgt tgttgccgac
     1261 ggtctgcaag gccgcgtagt tcgcgaacat cgcggtcttg ccgatcgata gaagcgcgga
     1321 catgccaaaa cctttcaggc cagcgactgc ggcgcgtgca ccgcggcccg ctgcaagcgc
     1381 agcgtggtgt tgatgacgcg ggtcagcttg tcggcatagg ccggatcggt ggcgtagccg
     1441 gcgcgttgca gcccctgcgc gaagccctgc gcgctgttgc cggcggcgac gaccccggcg
     1501 tagcgcgggt tgttcttcat cagctgcgcg tagtcgcgga acgactcggc atcgctcgcg
     1561 taggcgcgga acttcgccat caccttgcgc ggctggccgt cgatcacctc ggtcgtcatc
     1621 acctgcgcaa cccggccctg ccagcccgcg ccggccttga tgccgaacag gttgtgcgcg
     1681 ctcgtgccgt cttcgtggcg aatctcgcgc cggccccagc ccgattcgtg cgccgcctgc
     1741 gcgaccatga aggtcgccgg gatgccggtc tcggcctcgg caccggcggc cgccgcggtg
     1801 tgccggcgca ggaactcctg ctggttcggc gtgacgttcg cgagcttggc cgtcggcgcg
     1861 ccggccgcac ccgcggcggg cgccgcgacc tgcggcgcga cgccgttcat ctggcgctct
     1921 agctggcgcg cgatcacgtc ggccaggccg ccgggcgcgc cgctcatctt gttcgcgaac
     1981 tgggtgtcga gcatctcggt gcccatctgc gtgcccgcgt tgtcgagcat gccggtgccg
     2041 agcgacgact cgcgcatgct cttcatcagc tgctgcatga acatcgcctc gaactgcttc
     2101 gcggccatgc gaatcgccgc tttcggatcg cttgctgcgc gcgcgcgcag cgcgctgccc
     2161 gtcgcgtcca gcgcgccggc gtcgccggtc gttgcggagt agccggagat ggccatgttc
     2221 agatcacttc gatctcggcg ttgagggcgc cggcggtctt catcgcctgc aggatcgcga
     2281 gcaggtcctg cggcgtcgcg ccgagggtgt tgagcgcctt gacgacgtcg gcgagcttgg
     2341 ccccgggcgc c
//
LOCUS       DASRDG010000551         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1237394, whole genome shotgun sequence.
ACCESSION   DASRDG010000551 DASRDG010000000
VERSION     DASRDG010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237394"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VFA35_09235"
     CDS             <1..617
                     /locus_tag="VFA35_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZT56397.1"
                     /translation="LVATSPNVLVVDPKLGANTLADFVRIAKEKKGQLNYGSVGNGSA
                     SHLAMELFKTRAGLDIVHVPYQGFPQVVGAILAGQVQAGFMVPGIAIGNIRAGKLKAL
                     GVTTLGRSASLPELPSFVEQGYAGFEAISWQAVLAPAGTPKAIVDRVADALIRIIKTD
                     DVRARMLGQFFSAAGTAPEALAGLMKSERERWAQVIKAAGVQPE"
     gene            complement(618..1088)
                     /gene="trmL"
                     /locus_tag="VFA35_09240"
     CDS             complement(618..1088)
                     /gene="trmL"
                     /locus_tag="VFA35_09240"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HZT56398.1"
                     /translation="MFNIVLVRPEIPPNTGNVIRLAANTGCALHLIEPLGFSMDDRQL
                     KRAGLDYHEYAPVRTHASWAAFIAAMQPDPTRLVAFTTRGSRPFAELAWHAGDWLVFG
                     SETAGLPAEVRDSFDAERRVRLPLRAGQRSLNLSNAVAVAVFEAWRQNGYAGSV"
     gene            complement(1099..1809)
                     /locus_tag="VFA35_09245"
     CDS             complement(1099..1809)
                     /locus_tag="VFA35_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZT56399.1"
                     /translation="MLFTRLLRAARVPSLCAVCRGWGSQRVCAECVGRFAAPVPRCRR
                     CALQVPAGVACCGACLTDPPPCARTLAAVDYDHPWDGLITHFKFHDALDLAPVLAARM
                     LAARGDAPAPALLVPIPLSAARLRERGYNQAWELAKRLGAALDCPTDPTLLLRVKDTP
                     HQLAFPPAQRAGNVRAAFALEPRRRGELAGCSVTLVDDVMTTGATLAEAARTLLQAGA
                     GEVNAWVVARTPRPDAQP"
     gene            1830..>2617
                     /locus_tag="VFA35_09250"
     CDS             1830..>2617
                     /locus_tag="VFA35_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HZT56400.1"
                     /translation="MPAAPAPLDPNPARRLDPTAVRTALRRLARAPEAPWLHGEIARR
                     MGEKLELILLRPERVIDWWSALGAGAGVLAAAYPKAQQLRVEPDAAWAARSRALAARP
                     WWRAARWTGADVTVATEADEIAPGAGLVWANMMLHAVVDPAALIERWHGVLRVDGFVM
                     FSCLGPGTLRELRTLYERLGWPAPTPGFVDMHDLGDMLVRAGFKDPVMDQETLTLRWP
                     NPRALLAELRTLGANAAPDRSDGLRTPRWRAQLERELAALAGPDG"
BASE COUNT          354 a          940 c          987 g          336 t
ORIGIN      
        1 cgctcgtcgc gacttcgccg aacgtgctcg tcgtcgatcc gaagctcggc gcgaacacgc
       61 tcgccgactt cgtgcgcatc gcgaaggaga agaagggcca gctgaactac ggcagcgtcg
      121 gcaacggcag cgcgtcgcac ctcgcgatgg aactgttcaa gacgcgtgca gggctcgaca
      181 tcgtgcacgt gccgtaccaa ggctttccgc aggtcgtggg tgcgatcctg gcgggccagg
      241 tgcaggccgg cttcatggtg ccgggcatcg cgatcggtaa catccgcgcc ggcaagctga
      301 aggcgctcgg cgtcacgacg ctgggccgct cggcctcgct gccggaactg ccgagcttcg
      361 tcgagcaggg ctacgccggc ttcgaggcga tctcgtggca ggcggtgctc gcaccggccg
      421 gcacgcccaa ggcgatcgtc gaccgcgtgg ccgatgcgct gatccgcatc atcaagaccg
      481 acgacgtgcg cgccaggatg ctcggccagt tcttcagcgc cgcgggcacc gcgcccgagg
      541 cgctggccgg gctgatgaag agcgaacgcg agcgctgggc tcaggtgatc aaggccgcgg
      601 gcgtccaacc cgaatagtca cacgctgccg gcgtagccgt tctggcgcca ggcctcgaac
      661 accgcgaccg cgaccgcgtt gctcagattc aggctgcgct ggccagcgcg cagcggcagg
      721 cgcacgcggc gctcggcgtc gaagctgtcg cgcacctcgg ccggcaggcc ggcggtttcg
      781 gaaccgaaca cgagccagtc ccccgcgtgc cacgccagct cggcgaacgg ccggctgccg
      841 cgcgtcgtga acgcgaccaa ccgcgtgggg tcgggttgca tcgccgcgat gaacgcggcc
      901 cacgacgcgt gcgttcgcac cggcgcgtac tcgtggtagt cgagcccggc gcgcttgagc
      961 tggcgatcgt ccatcgagaa gccgagcggt tcgatcaggt gcagcgcgca gccggtgttg
     1021 gcggccaggc ggatcacgtt gccggtgttc ggcgggatct cgggacgcac caggacgatg
     1081 ttgaacatgg ccggattgtc atggctgtgc gtcggggcgc ggcgtgcgcg cgacgaccca
     1141 ggcgttcacc tcgcccgcgc ccgcctgcag cagcgtgcgc gcggcttcgg ccagcgtggc
     1201 gccggtcgtc atcacgtcgt cgacgagggt gacgctgcag cccgcgagtt cgccacgccg
     1261 ccgcggctcg agcgcgaacg ccgcgcgcac gttgccggcg cgctgcgcgg gcgggaaggc
     1321 gagctggtgc ggcgtgtcct tcacgcgcag cagcagggtc gggtcggtcg ggcaatcgag
     1381 cgcggcgccg aggcgcttcg cgagctccca ggcctggttg tagccgcgct cgcgcaggcg
     1441 cgccgcgctg agcgggatcg gcaccagcag cgccggcgcc ggcgcgtcgc cgcgcgccgc
     1501 gagcatgcgc gcggcgagca cgggtgccag gtcgagcgcg tcgtggaact tgaagtgcgt
     1561 gatcaggccg tcccacgggt ggtcgtaatc gactgccgcc agcgtgcgcg cgcagggcgg
     1621 cggatcggtc aggcacgcgc cgcagcacgc gacgcccgcc ggcacctgca gcgcgcagcg
     1681 ccggcagcgc ggcactggcg ccgcgaagcg cccgacgcac tcggcgcaca cgcgctgcga
     1741 tccccagccg cggcagacgg cgcacaggct gggcacgcgg gcggcgcgga ggagtcgggt
     1801 gaacagcacg gctcaatata ctggcccgca tgcccgccgc tcctgcgccg ctcgacccga
     1861 accccgcccg ccggctcgac ccgaccgcgg tgcgcaccgc cttgcgccgc ctcgcgcgcg
     1921 cgcccgaagc gccgtggctg catggcgaga tcgcgcggcg catgggcgag aagctcgagc
     1981 tcatcctgct gcggcccgag cgcgtgatcg actggtggag cgcgctcggc gccggcgccg
     2041 gggtgctggc agccgcctac ccgaaggcgc agcagctgcg cgtcgagccc gatgcagcct
     2101 gggccgcgcg cagccgcgcg ctggccgcgc ggccgtggtg gcgcgcggca cgctggaccg
     2161 gcgccgacgt gacggtcgcg accgaggccg acgagatcgc ccctggcgcc gggctggtct
     2221 gggccaacat gatgctgcac gcggtcgtcg acccggccgc actgatcgag cgctggcacg
     2281 gcgtactgcg cgtcgatggc ttcgtgatgt tctcgtgcct cgggcccggc acgctgcgcg
     2341 agctgcgcac gctctacgag cgcctgggct ggccggcgcc gacgcccggt ttcgtcgaca
     2401 tgcacgacct cggcgacatg ctggtgcggg ccggcttcaa ggacccggtg atggaccagg
     2461 agacgctgac gctgcgctgg ccgaatccgc gcgccttgct ggccgagctg cgcacgctcg
     2521 gcgccaatgc cgcccccgac cgcagcgacg gcctgcgcac gccccggtgg cgggcgcagc
     2581 tggagcgcga actcgcggcg ctggcggggc ccgatgg
//
LOCUS       DASRDG010000552         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1691074, whole genome shotgun sequence.
ACCESSION   DASRDG010000552 DASRDG010000000
VERSION     DASRDG010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691074"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFA35_09255"
     CDS             <1..180
                     /locus_tag="VFA35_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HZT56401.1"
                     /translation="EPEHGIVSIGSGGAYAHASARALLSNTDLAPHEVVKKSLEIAGE
                     LCIYTNQNHIIETLE"
     gene            177..1532
                     /gene="hslU"
                     /locus_tag="VFA35_09260"
     CDS             177..1532
                     /gene="hslU"
                     /locus_tag="VFA35_09260"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZT56402.1"
                     /translation="MKSSMTPQEIVSELDRHIVGQAGAKRAVAIALRNRWRRQQVAEP
                     MRAEITPKNILMIGPTGVGKTEIARRLAKLADAPFIKVEATKFTEVGYVGKDVDSIVR
                     DLVDMAVKQERESALRRHRTRAEDAAEDRVLDILVPPARSTNNEFGLTASVPKTEPDN
                     TARQVMRKRLREGLLADKEIEIEVADPKPSLEIMGPPGMEEMTEQLKGLFANAGSAKK
                     KVRKLRIAEAHKLLIEEEAAKLVNDDEVKTAALANAEQNGIVFIDEIDKVTSRSDAGG
                     GAEVSRQGVQRDLLPLVEGTTVNTKYGMVKTDHMLFIASGAFHLAKPSDLIPELQGRF
                     PIRVELGSLSVDDFEAILTQTHASLVKQYTLLLATEGVTLELQPEAIRRIAQIAFDVN
                     ERTENIGARRLSTVMERLLDEISFDAPNRGGQTVRVDAAEVNAKLAELAKNEDLSRYI
                     L"
     gene            complement(1597..2178)
                     /locus_tag="VFA35_09265"
     CDS             complement(1597..2178)
                     /locus_tag="VFA35_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZT56403.1"
                     /translation="MAALSTGAFAAANVLPGNWDVIQTFGTVGVLRAGSPWAGSASLA
                     AQSSIVDGVFVPEMQTWNTGSWWWDEDPSVNTSPVVTTIHLNQMFTLTGFAVQADDND
                     SYRLEYWDGSAWQLAWDVAPVYTFGLVTRTSGALPAITTDFLRFTATGGDNYYSVSEI
                     QAYGVAAVPEPGTYALMLAGLGVTGLIARRRRG"
BASE COUNT          470 a          795 c          798 g          332 t
ORIGIN      
        1 gaacccgagc acggcatcgt ctcgatcggc tcgggcggcg cgtatgcgca cgcgagcgcg
       61 cgcgcactgc tgtcgaatac cgacctggcg ccgcacgagg tggtgaagaa gtcgctcgag
      121 atcgcaggcg aactctgcat ctacacgaat cagaaccaca tcatcgagac gctcgaatga
      181 agagcagcat gacgccccaa gagatcgtct ccgaactcga tcgccacatc gtcggccagg
      241 ccggcgcgaa gcgcgcggtc gcgatcgcgc tgcgcaaccg ctggcgccgc cagcaggtcg
      301 ccgagccgat gcgcgcggag atcacgccga agaacatcct gatgatcggg ccgaccggcg
      361 tgggcaagac cgagatcgcg cggcgcctgg ccaagctcgc cgacgcgccg ttcatcaagg
      421 tcgaggcgac caagttcacc gaggtcggct atgtcggcaa ggatgtcgac tcgatcgtgc
      481 gcgacctggt cgacatggcc gtcaagcaag agcgcgagag cgcactgcgc cggcaccgca
      541 cacgtgccga agacgcggcc gaagaccggg tgctcgacat cctggtgccg ccggcccgga
      601 gcacgaacaa cgagttcggc ctgaccgctt cggtgccgaa gaccgagccc gacaacaccg
      661 cgcgccaggt gatgcgcaag cgcctgcgcg aagggctgct ggccgacaag gagatcgaga
      721 tcgaggtcgc cgacccgaag ccctcgctcg agatcatggg cccgcccggc atggaagaga
      781 tgaccgagca gctcaagggc ttgttcgcga acgccggcag cgcgaagaag aaggtgcgca
      841 agctgcgcat cgccgaggca cacaagctgc tgatcgaaga agaagccgcc aagctcgtca
      901 acgacgacga ggtcaagacc gccgcgctcg cgaacgccga gcagaacggc atcgtcttca
      961 tcgacgagat cgacaaggtc acttcgcgct cggatgccgg tggcggcgcc gaggtctcgc
     1021 gccagggcgt gcagcgcgac ctgctgccgc tggtcgaagg caccaccgtc aacaccaagt
     1081 acggcatggt caagaccgac cacatgctgt tcatcgcgtc gggcgcgttc cacctggcca
     1141 agccgagcga tctgatcccc gagctgcagg gccgcttccc gatccgcgtc gaactgggtt
     1201 cgctgagcgt cgacgacttc gaggcgatcc tgacgcagac ccacgccagc ctggtcaagc
     1261 agtacacgct gctgctcgcg accgagggtg tgacgctgga actgcagccc gaggcgatcc
     1321 gccgcatcgc gcagatcgcg ttcgacgtca acgagcgcac cgagaacatc ggcgcgcggc
     1381 gcctgtcgac cgtgatggag cggctgctcg acgagatcag cttcgacgcg ccgaaccgcg
     1441 gtggtcagac ggtgcgcgtc gatgcggccg aggtcaacgc caagctcgcc gaactggcga
     1501 agaacgaaga cctgtcgcgc tacattctct gatccgagcg tgcgcgagaa aaaagcgaac
     1561 ccgacgggct cgctctcggt cgagcaacgc ccgacgtcag ccgcggcggc gccttgcgat
     1621 caagcccgtc acgcccagcc cggccagcat cagcgcatag gtgccgggtt cgggcaccgc
     1681 ggcgacacca taggcctgaa tctcggaaac gctgtagtag ttgtcgccgc cggtcgcggt
     1741 gaagcgcagg aagtcggtag tgatcgcagg cagcgcaccc gaggtgcgcg tcaccagccc
     1801 gaaggtataa accggcgcca cgtcccacgc cagttgccaa gccgaaccgt cccagtactc
     1861 gaggcgatag ctgtcgttgt cgtcggcttg caccgcaaag ccggtgagcg tgaacatctg
     1921 gttcagatgg atcgtggtca cgaccggcga cgtgttgacg ctcgggtctt cgtcccacca
     1981 ccagctgccg gtgttccagg tctgcatttc cggcacgaac acgccgtcga caatcgagga
     2041 ctgtgccgcg agcgacgcgc tgccggccca cggtgagccg gcacgcagca caccgaccgt
     2101 accgaaggtc tgaatgacgt cccaattgcc gggcaggacg ttggcggcag caaacgcgcc
     2161 ggtcgacaag gcggccaagg cggcgcccgc gagggcagtg cggatacgga tgttcatggc
     2221 tggttgctcc tggggtcgag ttgatgcgga tcgcacgtgt cgcccgccgg ccattgttga
     2281 ggcgacctcg cagctcggct cccccaaagg gtaggggcga ccccgcgatc cgggggctcg
     2341 gtgcgggcag ctcgcccgcg agccgtgtcg gcttacagct atagcgccaa tcgat
//
LOCUS       DASRDG010000553         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1468777, whole genome shotgun sequence.
ACCESSION   DASRDG010000553 DASRDG010000000
VERSION     DASRDG010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468777"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2228
                     /locus_tag="VFA35_09270"
     CDS             <1..2228
                     /locus_tag="VFA35_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT56404.1"
                     /translation="IEVAHHLRDEMERLRPTLPKDIDMRLAYDATVFMEDALTEITKT
                     LGETVLIVGIVVFLFLGSVRTAIVPLLAMPVSLIGAAAVMLACGFSLNLLTILAIVLS
                     VGLVVDDAIVVVENIARHVREGKARLDAALLGARELVGPIIAMTITLAAVYAPIGFQG
                     GLTGSLFLEFAISLAAAVVLSGIVAVTLSPVMSAHFVHPHGREGWLTRLVDRAFDLVR
                     RAYAVLLDGALRRRGAVVAAAVLAMSAAVPFYQHSRRELAPVEDQSHISLFLDAAPDS
                     TLHAVNGESRKVVNAITSFPEAKFMWSLCASWGGFGGMVAKDWHERARSTEQMYGAVF
                     GAVSQVPGLRVFPRLDPPLPNAGQYDVELVLQSAVPPEQMLATAAAVVGAGWQSGKFL
                     YVDTDLKVDRPEARVVIDREQIADLGLDLASVGQELGTMLGGAYVNRFNWFDRSYKVI
                     PQLGDEDRATVGPLLDLKIRTPGGQLVPVSTFTRIESGTEPRSLNRFQQRNALRVFGG
                     VKPGVTKEQGLAVLEQAAAAAALPDQQMDYAGESRQIRREGSALTVTLGFAVVLIYLV
                     LAAQFQSFRDPLIVLLGSVPLAVSGALAFSFLDLTTINIYSQVGLITLVGLIAKNGIL
                     IVEFANKLQARGVDKTAALREASLTRLRPVLMTSAATVFGHFPLVLVTGPGAHARNSI
                     GTVLVAGMVVGTLFTLFVVPVFYSLIAARRAPAPERLEDAHAAIAAPPLLAPVLAEAV
                     S"
     gene            2225..>2465
                     /locus_tag="VFA35_09275"
     CDS             2225..>2465
                     /locus_tag="VFA35_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HZT56405.1"
                     /translation="MIASTPRLGIPRLVAVLAVGACSAVGPDYHPPTAAVPDAFRETG
                     AVLVAAEPDLAQWWHRLDDAVLDGLVEQATRQNLDL"
BASE COUNT          320 a          920 c          843 g          382 t
ORIGIN      
        1 agatcgaggt cgcccaccac ctgcgcgacg agatggagcg gctgcggccg acgctgccca
       61 aggacatcga catgcggctc gcctacgacg cgacggtgtt catggaggac gcgctgaccg
      121 agatcaccaa gacgctcggc gagacggtgc tgatcgtcgg catcgtcgtg ttcctgttcc
      181 tcggctcggt gcgcacggcg atcgtgccgc tgctggcgat gccggtgtcg ctgatcggcg
      241 ccgccgccgt gatgctggcg tgcggcttca gcctgaacct gctgacgatc ctcgcgatcg
      301 tgctgtccgt cggcctggtc gtcgacgacg cgatcgtcgt cgtcgagaac atcgcgcgcc
      361 acgtgcgcga agggaaggcg cggctcgacg cggcgctgct cggcgcgcgc gagctggtcg
      421 gcccgatcat cgcgatgacg atcacgctgg cggccgtcta cgcgccgatc ggcttccagg
      481 gcgggctcac gggctcgctg ttcctcgagt tcgcgatctc gctggcggcc gccgtcgtgc
      541 tgtccggcat cgtcgccgtg acgctgtcgc cggtgatgag cgcgcacttc gtgcatccgc
      601 acggcaggga agggtggctc acgcgcctcg tcgaccgcgc cttcgacctg gtgcggcgcg
      661 catacgccgt cctgctcgac ggcgcgctgc ggcgccgcgg cgccgtcgtc gccgccgccg
      721 tgctcgccat gagcgccgcg gtgccgttct accagcactc gcgccgcgaa ctcgcgccgg
      781 tcgaggacca gagccacatc agcctgttcc tcgacgcggc gcccgactcg acgctccacg
      841 ccgtcaacgg cgagtcgcgc aaggtcgtga atgcgatcac cagcttcccc gaggcgaagt
      901 tcatgtggtc gctctgcgcc tcgtggggcg gcttcggcgg catggtcgcc aaggactggc
      961 acgaacgcgc gcgctcgacg gagcagatgt acggcgcggt gttcggcgcc gtgtcgcagg
     1021 tgcccggcct gcgggtgttc ccgcgcctcg acccgccgct gcccaacgcc ggccagtacg
     1081 acgtcgagct cgtgctgcag agcgccgtgc cgccggagca gatgctcgcg accgcggccg
     1141 ccgtcgtcgg cgccggctgg cagagcggca agttcctcta cgtcgacacc gacctcaagg
     1201 tcgaccggcc ggaggcgcgc gtcgtcatcg accgcgagca gatcgccgac ctcggcctcg
     1261 acctcgcgtc ggtgggccag gagctcggca ccatgctcgg cggcgcctac gtcaaccgct
     1321 tcaactggtt cgaccgcagc tacaaggtca ttccgcagct cggcgacgag gaccgcgcga
     1381 cggtcggccc gctgctcgac ctcaagatcc gcacgcccgg cggccagctg gtcccggtct
     1441 ccacgttcac gcgcatcgag tccggcaccg agccgcgcag cctcaaccgc ttccagcagc
     1501 gcaacgcgct gcgcgtgttc ggcggcgtca agcccggtgt caccaaggag cagggcctcg
     1561 ccgtgctcga gcaggcggcg gcggccgcgg cgctgcccga ccagcagatg gactacgccg
     1621 gcgaatcgcg gcagatccgc cgcgagggct cggcgctcac cgtcacgctc ggcttcgccg
     1681 tcgtgctcat ctacctggtg ctcgccgcgc agttccagag cttccgcgac ccgctgatcg
     1741 tgctgctcgg ctcggtgccg ctcgcggtgt ccggcgcgct ggcgttcagc ttcctcgacc
     1801 tgacgacgat caacatctac tcgcaggtcg ggctgatcac gctggtcggg ctgatcgcca
     1861 agaacggcat cctgatcgtc gagttcgcca acaagctgca ggcgcgcggc gtcgacaaga
     1921 ccgcggccct gcgcgaagcc tcgctgacgc gcctgcggcc ggtgctgatg acctcggcgg
     1981 cgaccgtgtt cggccacttc ccgctggtgc tggtcacggg ccccggcgcc cacgcccgca
     2041 acagcatcgg caccgtgctg gtcgccggca tggtcgtcgg cacgctgttc acgctgttcg
     2101 tcgtgccggt cttctactcg ctgatcgcgg cgcggcgcgc gcccgcgccg gaacgactcg
     2161 aggacgcgca cgcagcgatc gccgcgccgc cgctgctcgc gcccgtgctc gcggaggcag
     2221 tgtcgtgatc gccagtaccc cccgattggg catcccccgg ctggtcgccg tgctcgcagt
     2281 cggcgcctgc agcgccgtcg gccccgacta ccacccgccg accgcagccg tgccggacgc
     2341 gttccgcgag accggggccg tgctggtcgc cgccgaaccc gatctcgcgc agtggtggca
     2401 tcgcctcgac gacgccgtgc tcgacggcct cgtcgagcag gcgacgcgcc agaacctcga
     2461 cctca
//
LOCUS       DASRDG010000554         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1011139, whole genome shotgun sequence.
ACCESSION   DASRDG010000554 DASRDG010000000
VERSION     DASRDG010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011139"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /gene="lnt"
                     /locus_tag="VFA35_09280"
     CDS             <1..606
                     /gene="lnt"
                     /locus_tag="VFA35_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZT56406.1"
                     /translation="DYTNSVFGVGPQFQGVYRYNKHHLVPFGEFIPFGFRWFTEMMNI
                     PLGDFNRGPISAASFAFRGERVGANICYEDLFGEELAARFRAPAGAPTIFANISNIGW
                     FGDTAAITQHLQISRMRALEFQRPMLRATNTGATAVIDHTGRVTASLPRLTQGVLDGE
                     VQGRTGLTPFAWWASRWGLWPPTLLALLIVASAVFARRREP"
     gene            661..1577
                     /gene="glyQ"
                     /locus_tag="VFA35_09285"
                     /pseudo
     CDS             661..1577
                     /gene="glyQ"
                     /locus_tag="VFA35_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
     gene            1581..>2630
                     /gene="glyS"
                     /locus_tag="VFA35_09290"
     CDS             1581..>2630
                     /gene="glyS"
                     /locus_tag="VFA35_09290"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZT56407.1"
                     /translation="MTHATPTRNLLVELFVEELPPKALKKLGEAFAGALAAGLRAQGL
                     ATDASVVTPFASPRRLAAHVTSVSAQAAAKAVSHKLMPVGVGLNAAGEATPALLKKLA
                     AIGADASAVPQLKRRIDGKAEALFCDSVVTGATLAEGLQTALDEALAKLPIPKVMQYQ
                     LADGWTSVNFVRPAHGLVALHGDAVVPVAALGLTAGRTTHGHRFEAAADPIELQHADA
                     YAEALAAQGAVIASFDARRAEIARQLQAAAARQRLTPIDDAALLDEVTALVERPNVLV
                     CRFEPEFLAVPPECLILTMKANQKYFPLLDAAGRLSNRFLIVSNISPQDPAAVIGGNE
                     RVVRPRLADAKFFFEH"
BASE COUNT          431 a          924 c          888 g          387 t
ORIGIN      
        1 gattacacca acagcgtgtt cggcgtcggc ccgcagttcc agggcgtgta ccgctacaac
       61 aagcaccacc tggtgccgtt cggcgagttc atcccgttcg gttttcgctg gttcaccgag
      121 atgatgaaca tcccgctcgg cgacttcaac cgcgggccga tcagcgccgc gtcgttcgcg
      181 ttccgcggcg aacgcgtcgg cgcgaacatc tgctacgagg atctgttcgg cgaagagctc
      241 gcggcgcgct tccgcgcccc ggccggcgcg cccacgatct tcgcgaacat cagcaacatc
      301 ggctggttcg gcgacacggc cgcgatcacg cagcacttgc agatttcgcg catgcgcgcg
      361 ctcgagttcc agcgcccgat gctgcgcgcg accaacaccg gcgcgaccgc ggtgatcgac
      421 cacaccggcc gcgtcaccgc gagcctgccg cggctgacgc agggcgtgct cgatggcgag
      481 gtgcagggtc gcaccggcct cacgccgttc gcgtggtggg catcgcgctg gggcctgtgg
      541 ccgccgacgt tgctcgcgct gctgatcgtc gcgagcgcgg tgttcgcgcg gcggcgcgag
      601 ccttgagcgg cgccccggcc ctctagaatc gagcgtttac ggtagaaccc gacggcctcg
      661 atgctcacct tccagcagat cattctcaag ttgcaggact actggggttc gcagggttgc
      721 gcgctgctcc agccctacga catggaagtg ggcgcgggca ccagccacac cgcgaccttt
      781 ctgcgcgcga tcggccccga gccctggaag gccgcctacg tgcagccgag ccgccgcccc
      841 aaggacggcc gctacggcga gaacccgaac cggctgcagc actactacca gtaccaggtc
      901 gtgctcaagc ccgcgccggc cgacatcctc gagctctacc tgggctcgct cgaagcgctc
      961 ggcttcgacc tgaaggccaa cgacatccgc ttcgtcgaag acgactggga gaacccgacg
     1021 ctgggctgct ggggcctggg ctgggaggtc tggctgaacg gcatggaggt acgcagttca
     1081 cctacttcca gcaggtcggc ggcatcgact gcaagccgat caccggcgag atcacctacg
     1141 gcctggagcg gctcgcgatg tacctgcagg gcgtcgacaa cgtctacaac ctgacctgga
     1201 ccgaggggct gagctacggc gacgtctaca agcagaacga ggtcgaacaa agcacctaca
     1261 acttcgagca cagcgatgtc gactttctgc tgaccgcgtt cgcagcgcac gaaaaacagt
     1321 cgaaacactt gatggcgcag caactcgcgc tgccggccta cgagcaactg ctgaaggccg
     1381 gccacagctt caacctgctc gacgcgcgcg gcgcgatcag cgtgaccgag cgggccgcct
     1441 acatcgggcg catccgcaac ctcgcgcgtg ccgtcgcgca gagctatctc gagagccgcg
     1501 cgcgcctggg tttcccgatg gcgccgcggg cctgggccga tgaagtcgcc gcgacgctgg
     1561 cgaagaaggc ggcctgatcg atgacgcatg cgaccccgac ccgcaacctg ctcgtcgaac
     1621 tgttcgtcga agaactcccg ccgaaggcgc tgaagaagct gggcgaggcg ttcgccggcg
     1681 cgctcgccgc cggcctgagg gcgcagggcc tcgcgaccga cgcctcggtt gtcaccccgt
     1741 tcgcatcgcc gcgtcgcctg gcggcgcatg tcacgtcggt gagcgcgcag gccgccgcca
     1801 aggccgtgtc gcacaagctg atgccggtcg gcgtcggcct gaatgccgcg ggcgaagcga
     1861 cacccgcgct gctgaagaag ctcgcggcca tcggcgccga tgcgtcggcc gtgccgcaac
     1921 tcaaacgccg catcgacggc aaggccgagg cgctgttctg cgacagcgtc gtgaccggcg
     1981 cgaccctggc cgagggcctg cagacggcac tcgacgaggc gctcgcgaag ctgccgatcc
     2041 cgaaggtgat gcagtaccag ttggccgacg gctggaccag cgtgaacttc gtgcgcccgg
     2101 cgcacgggct ggtcgcgctg catggcgacg cggtcgtgcc ggtcgcggcg ctgggcctga
     2161 cggcaggccg cacgacgcac ggccaccgct tcgaggccgc ggccgacccg atcgaattgc
     2221 agcatgccga cgcctacgcc gaggcactgg ccgcgcaggg cgcggtgatc gccagcttcg
     2281 acgcgcgccg cgccgagatc gcgcgccagc tgcaggccgc ggccgcgcgg cagcggctga
     2341 cgccgatcga cgacgcggcg ctgctcgatg aagtcaccgc gctggtcgag cggccgaatg
     2401 tgctggtgtg ccggttcgag cccgagttcc tcgccgtgcc gcccgagtgc ctgatcctga
     2461 cgatgaaggc caaccagaag tacttcccgc tgctcgacgc ggcgggccgc ctgagcaacc
     2521 gctttctgat cgtgagcaac atcagtccgc aggacccggc cgcggtgatc ggcggcaacg
     2581 agcgggtggt gcggccgcgt ctggcggacg ccaagttctt cttcgagcac
//
LOCUS       DASRDG010000555         4014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_316902, whole genome shotgun sequence.
ACCESSION   DASRDG010000555 DASRDG010000000
VERSION     DASRDG010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316902"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..895)
                     /locus_tag="VFA35_09295"
     CDS             complement(116..895)
                     /locus_tag="VFA35_09295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZT56408.1"
                     /translation="MTTLYPSLKDRTVFVSGGSSGIGAELVRSFAAQGATVAFCGLRA
                     DGGAALVDEIKAAGHATPFFAPCDVRDVAAYQALLAEVGVRCGPIRVLVNNAGRDDRH
                     AMEEVTSEYWDDRLALNLKHYFFAIQAVAPGMAQAGGGSVVNMGSVSWMRGRPNLAGY
                     TTAKAGILGLTRTLARELGTRNIRVNAIVPGAIVTERQTTLHRDPAADQAFLDAQCLK
                     IRLDPGHVARPTLFLAADDSDGITGQHILVDAGIAQQSIIS"
     gene            complement(898..1773)
                     /locus_tag="VFA35_09300"
     CDS             complement(898..1773)
                     /locus_tag="VFA35_09300"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZT56409.1"
                     /translation="MPIEPSLLGESPLWHPHEQALYWCDIPGRRLNRYDPAAQQHRDW
                     TFDTDVACCAPHAGGGLVLALRDGLWRFDPIGNTRTKLADAPYDTHVERFNDGKADTG
                     GRLWVGTIYEPRDTPSAALYRWADAKLDRVAADITVSNGLAWSPDSATMYWADTKAHT
                     VYTCDFDATHGVPSNRRVFMRFATREPNTPLDRYGGRPDGATVDAEGCYWIAMFEGAR
                     VLRLSPRGELLRTIHLPVRCPTMPCFGGPDLRTLYITTARENRPADELTAMPWSGCVL
                     ELRVDVPGLPANIAR"
     gene            complement(1821..2936)
                     /locus_tag="VFA35_09305"
     CDS             complement(1821..2936)
                     /locus_tag="VFA35_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZT56410.1"
                     /translation="MNQTIRYGIIGCGSMGREHIENIRALGSGVVTAIADPDAGSREQ
                     AAALAGGSVQLFEDHRALLDSGLCDALVVASPNFTHVAVMRDALATDLHILCEKPLVT
                     KVADGIEMIELARKRKGIVWVAQEYRYMPPVAEMIRLAHDGAVGTLHQVAIREHREPF
                     YPKVGDWNRFNANTGGTLVEKCCHYFNLMDFILKEKALRVFASGGQRVNHLDEKYDGK
                     TPDILDSAYVIVEYPSGARASLDLCMFAENSVDNEQVVIVGAEGKLESLLPSGVLRFG
                     RREDWGRREVWGQPSGTAKGVSVRQIRDTNIKYLGQHFGASYVEHQKFARAVREGLPS
                     EIPLEEGLRAVATGLAAHRSIETGLPVAIGDLLPAGL"
     gene            complement(3007..>4014)
                     /locus_tag="VFA35_09310"
     CDS             complement(3007..>4014)
                     /locus_tag="VFA35_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZT56411.1"
                     /translation="VSKVKRLDSGTYTASRLIFRGVEDLGDGLTAGFYLETRFNADVG
                     AQQSATKFFNAGSQVYLADKKWGSLTLGRQYVPIFWSFLFADDTGPLRLHGYSALQSV
                     QRSAFARISAAASPVKAAGSLDTISGGIYQLGITSAFEDNLVVYKTPVFGGATAMLAV
                     GAAEGAGPGSGRVLGGNVEYRGGPFYGSVAFNQKRGTVPAGGSGAYQTQTEELVSALY
                     EIMPNQLKVWGNFHPWKLQSPGGDLKGHDWMLGASYWFSQSELWVNYAAKTLDNCAAC
                     NSKGFGLGYHYFMSRRTELYASVAQVANDANSGNTLNGFAPGTLGKKVKGYAVGIAHT
                     F"
BASE COUNT          608 a         1360 c         1393 g          653 t
ORIGIN      
        1 tcgaccgcga cgccgaggcc ggtcgccccg ccgccgatca cgacgacatc ccattcggcg
       61 ggggccatca gccgcgcgag caacgtggcg cgatgggtca cggcaggctt catcgtcagc
      121 tgatgatgga ttgctgcgcg atgccggcat cgaccaggat gtgctggccg gtgatgccgt
      181 cgctgtcgtc ggccgccagg aacagggtcg gccgcgcgac gtgacccgga tcgagccgga
      241 tcttcaggca ctgcgcgtcg aggaaggcct ggtccgcggc cgggtcgcgg tgcagcgtgg
      301 tctggcgctc ggtcacgatc gcgccgggaa cgatcgcgtt gacgcggatg ttgcgcgtgc
      361 cgagctcgcg cgcgagcgtg cgcgtgaggc cgagaatgcc cgccttggcg gtcgtgtagc
      421 cggccaggtt cggccgcccg cgcatccagg acaccgagcc catgttgacg accgagccgc
      481 cgccggcctg cgccatgccg ggcgcgacgg cctggatcgc gaagaagtag tgcttcaggt
      541 tcagggcaag acggtcgtcc cagtactcgg acgtcacctc ctccatcgcg tgcctgtcgt
      601 cgcggccggc gttgttgacg agcacgcgaa tcggaccgca acgcacgccg acctcggcga
      661 gcagggcctg gtaggcggcc acgtcgcgca cgtcgcacgg cgcgaagaac ggcgtggcgt
      721 ggccggcggc tttgatctcg tcgaccagtg ccgcgccgcc gtcggccctc aggccgcaga
      781 acgcgacggt cgcgccctgc gccgcgaagc tgcgcaccag ctccgcgccg atgcccgaac
      841 tcccgccgga aacgaagacg gtgcgatctt tcaatgaagg gtaaagcgtg gtcatggtca
      901 tctcgcaata ttggccggca agcccggcac gtcgaccctc agttccagca cgcagcccga
      961 ccacggcatc gccgtcagtt cgtcggccgg ccggttctcg cgggcggtgg tgatgtagag
     1021 cgtgcgcaga tcggggccgc cgaagcacgg catggtcggg caccgaaccg gcagatgaat
     1081 cgtgcgcagc agttcgccgc gcggcgacag gcgcagcacg cgcgcgccct cgaacatcgc
     1141 gatccagtag cagccttcgg catcgacggt cgcgccgtcg ggtcgaccgc cgtaccgatc
     1201 gagaggggta ttcggctcgc gcgtcgcgaa gcgcatgaac acgcgccggt tcgacggcac
     1261 gccgtgcgtg gcgtcgaagt cgcacgtgta gaccgtgtgc gccttcgtgt cggcccagta
     1321 catcgtcgcg ctgtcggggc tccacgccag gccgttgctg accgtgatgt cggcggcgac
     1381 gcgatcgagc ttcgcgtcgg cccatcgata cagcgcggcg ctcggcgtgt cgcgaggctc
     1441 gtagatcgtg ccgacccaca ggcgtccgcc cgtgtcggcc ttgccatcgt tgaagcgctc
     1501 gacgtgcgtg tcgtagggcg cgtccgcgag cttcgtgcga gtgttgccaa tgggatcgaa
     1561 gcgccacaag ccgtcgcgca gcgccagcac caggccgccg cccgcatgtg gcgcgcagca
     1621 ggccacgtcg gtgtcgaacg tccagtctcg atgctgttgc gcggccgggt cgtagcggtt
     1681 caggcggcgc cctggaatgt cgcaccagta gagcgcctgc tcgtgcggat gccacagcgg
     1741 cgactcaccg agcaggctcg gctcgatggg caagggtcga atcgacatcg cggcgctcgt
     1801 cgttcgcagg cagacggcgg ctacaggccc gccggcagca ggtcgccgat cgccaccggc
     1861 aacccggtct cgatgctgcg gtgcgcggcc aggccggtgg ccacggcgcg caggccttcc
     1921 tcgagcggga tctcggacgg caggccctcg cgcaccgccc gcgcgaactt ctggtgctcg
     1981 acgtagctgg cgccgaaatg ctggccgagg tacttgatgt tggtgtcgcg gatctggcgc
     2041 accgacacgc ccttcgcggt gcccgagggt tggccccaga cctcgcgccg gccccagtcc
     2101 tcgcgccggc cgaagcgcag cacgcccgaa ggcagcagcg attcgagctt gccctcggcg
     2161 ccgacgatca ccacctgctc gttgtcgacc gagttctcgg cgaacatgca caggtcgagc
     2221 gacgcgcgcg cgcccgaagg gtactcgacg atcacatagg cgctgtcgag gatgtcgggc
     2281 gtcttgccgt cgtacttctc gtccaggtgg ttgacgcgct ggccgccgga ggcgaacacg
     2341 cgcagcgcct tctccttcag gatgaagtcc atcaggttga aatagtggca gcacttctcg
     2401 accagcgtgc cgccggtgtt ggcgttgaag cggttccagt cgccgacctt ggggtagaac
     2461 ggctcgcggt gctcgcggat cgcgacctgg tgcagcgtgc ccaccgcgcc gtcgtgcgcg
     2521 aggcggatca tctctgccac cggcggcatg tagcggtact cctgcgcgac ccagacgatg
     2581 cccttgcgct tcctggcgag ctcgatcatc tcgatgccgt cggccacctt cgtcaccagc
     2641 ggcttctcgc acaggatgtg caggtcggtc gcgagcgcat cgcgcatcac cgcgacgtgg
     2701 gtgaagttcg ggctcgcgac cacgagcgca tcgcacaagc cgctgtcgag cagcgcgcgg
     2761 tggtcctcga acagctgcac cgagccgccc gccagcgcgg ccgcctgctc gcggctgccg
     2821 gcgtcggggt cggcgatcgc ggtgacgacg ccggagccga gggcgcggat gttttcgatg
     2881 tgctcgcggc ccatgctgcc gcaaccgatg atgccgtagc gaatggtctg attcatagct
     2941 tgctctggag ggttgagatc ggcccggtcg cgtcgtgacg gccgggcctt ccggttcgtt
     3001 ccgttgtcag aacgtgtgcg cgattccgac ggcgtagccc ttcaccttct tgccgagcgt
     3061 gcctggcgcg aagccgttca gcgtattgcc cgaattggcg tcgttggcga cctgcgcgac
     3121 cgacgcatac agctcggtgc ggcgcgacat gaagtagtgg tagcccaggc cgaagccttt
     3181 cgagttgcag gccgcgcagt tgtccagcgt cttggccgcg tagttcaccc acagctcgct
     3241 ctgcgagaac cagtacgagg cacccagcat ccagtcgtgc cccttgaggt cgccgcccgg
     3301 actctggagc ttccacgggt ggaagttgcc ccagaccttc agctggttcg gcatgatttc
     3361 gtacagcgcc gagaccagct cttcggtctg cgtttgatac gcgcccgagc cgccggcggg
     3421 aaccgtgccg cgcttctggt tgaacgcgac cgagccgtag aacggaccgc cgcgatactc
     3481 gacgttgccc ccgagtacgc gcccgctgcc ggggcccgcg ccttcggcag cgccgaccgc
     3541 cagcatggcg gtcgcaccgc cgaacaccgg cgtcttgtag acgacgaggt tgtcctcgaa
     3601 cgccgacgtg atgccgagct gatagatgcc cccggagatc gtgtcgagcg agcccgcggc
     3661 cttcaccggc gacgccgccg cggagatgcg tgcgaacgcg ctgcgctgga ccgactgcag
     3721 cgcgctgtag ccgtgcagcc gcagcggacc ggtgtcgtcg gcgaacagga aggaccagaa
     3781 gatcggcacg tactgccggc ccagggtcag cgacccccac ttcttgtcgg ccaggtagac
     3841 ctgcgagccg gcattgaaga acttggtggc gctctgctgt gcgccgacgt cggcattgaa
     3901 gcgggtctcc agatagaagc cggcggtcag gccatctccc aggtcttcga cgccgcgaaa
     3961 gatcaatcgc gatgcggtgt aggtgccgct gtcgagacgc ttgaccttgc tgac
//
LOCUS       DASRDG010000556         4665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1469162, whole genome shotgun sequence.
ACCESSION   DASRDG010000556 DASRDG010000000
VERSION     DASRDG010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469162"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFA35_09315"
     CDS             <1..328
                     /locus_tag="VFA35_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZT56412.1"
                     /translation="YGRYLAGPAGHCIECHSTPGADGRPDFEKSLGAGGMSFTGPWGV
                     SVAPSLRGANLRRYSDDQLKTIVTTGVRPDGSRLKPPMGVPYYANLTPADLDALVAFL
                     RSLPPL"
     gene            complement(358..795)
                     /locus_tag="VFA35_09320"
     CDS             complement(358..795)
                     /locus_tag="VFA35_09320"
                     /inference="COORDINATES: protein motif:HMM:NF027434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4826 family protein"
                     /protein_id="HZT56413.1"
                     /translation="MEANEDERWCDERAAEVAACMARLAPAHGRVGERPAWFAMPYAS
                     LWAVESAHRPEWIGWWAIAGDLPADALAAHELATPRDALRAFGQRWLRHAEQLDRGEV
                     PPAWSHLPDDALPKLAATLKRRGAALQVWADDATAWPADEDEA"
     gene            complement(806..1696)
                     /locus_tag="VFA35_09325"
     CDS             complement(806..1696)
                     /locus_tag="VFA35_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZT56414.1"
                     /translation="MPALETLPPHEKDLGGGFMVRRSLPSPRRQAVGPFVFFDHFGPV
                     TALPGDNFDVRPHPHIGLATVSYLFEGAMMHRDSTGVVQRIEPGAINLMTAGRGIVHS
                     ERTPEDLRAVERRSHGLQLWLAVPAEHEEDAPGFQHVPGAEIPAVDVNGVRVRVLIGS
                     AFGLRSPVATKSPTLYLDLSFAAGASLTIPDAAQERALYSVDHDFTLDGTPVPAFTMV
                     VLEPGSEPVLSAPPGGRIVLVGGAPLGPRFIVWNFVSSRKERIAQAQDDWEAFTSGRA
                     AGFEHVPGETEFIPLPPRRP"
     gene            1804..2895
                     /gene="ychF"
                     /locus_tag="VFA35_09330"
     CDS             1804..2895
                     /gene="ychF"
                     /locus_tag="VFA35_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZT56415.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     ELPDPRLQDLAAIVEPERIVPAIVEFVDIAGLVAGASKGEGLGNQFLAHIRETDAIVN
                     VVRCFDDENVIHVAGKVDPISDIEVIQTELCLADMGTVEKGLHRYNKVARSGDKEAQK
                     IVAVLEKCMAALNQGKPVRTIAFGKEDLALVKPLCLITAKPAMFVGNVDENGFENNPY
                     LDKLRAYAQGQGAPVVAICAKTEAELAEMDDADRLMFLSEMGQTEPGLNRLIRAAYTL
                     LGLQTYFTAGVKEVRAWTIHVGDTAPQAAGVIHTDFERGFIRAQTIAFADFVAFKGEQ
                     GAKDAGKMRAEGKDYVVKDGDVLNFLFNV"
     gene            3031..>4665
                     /locus_tag="VFA35_09335"
     CDS             3031..>4665
                     /locus_tag="VFA35_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HZT56416.1"
                     /translation="MATDTPTDKPLHWTAAFALTALCYALAGIGALPLAIPPGYSTPL
                     YPAAGVALASVLLFGPRMLPAVALGALSVNLSLALQRHAFSPALLPVPLAIAVGAALQ
                     ALAGALLAKRFVRQPLTLSEPRDIAVFLLTAMVSCLVSPVIGNLALWTAGSVPTAELP
                     FSASTWWIGDLLGVLIATPIILTLFGRPRDAWVPRRLSVGLTLALVTLLLALGIRQVA
                     QWNNERVQTAFDHDASNAAQALAAQLREPLQALEALNGVFLAAEHVSAEAMRLATRSW
                     LAPGRLQAMTWTERVARGDLPAFEARVRVEPGMERFKVFDRQDAAGASPRAGAPDDDM
                     LVVRYIEPPAGNTGAIGVNAMSVAAARGAIERTRDTGAPAASGGFRLTQQAAHESQLG
                     IVVYQAVYAPRAPAPAQRAAALRGTVSVVLRMDDQLQALAAQLPPYLALCLLDVSPGE
                     APRRLAGAPGCETRQAPLQLARALPYAGRRWEVRVTPAPGMQPNVHSADVWLFALVGL
                     GSTAMLGGFLLTVTGRTRRIETAVRERTAALQAEVRE"
BASE COUNT          660 a         1708 c         1659 g          638 t
ORIGIN      
        1 ctacggtcgc tacctcgccg ggccggccgg gcactgcatc gagtgccatt cgacgccggg
       61 cgccgacggc cggcccgact tcgagaagag cctcggggcc ggcggcatgt cgttcaccgg
      121 gccgtggggc gtcagcgtcg cgccgagcct gcgcggcgcg aacctgcggc gctactccga
      181 cgaccagctc aagacgatcg tcacgaccgg cgtgcgcccc gacggtagcc ggctgaagcc
      241 gccgatgggc gtgccgtact acgccaacct gacgccggcc gacctggacg cgctggtcgc
      301 gttcctgagg tcgctgccgc cgctgtagcg atcacacgcc cgccgcgccg cggcggctca
      361 ggcctcgtct tcgtccgccg gccacgcggt cgcgtcgtcg gcccagacct gcagcgccgc
      421 gccgcgccgc ttcagcgtcg cggcgagctt gggcagcgcg tcgtcgggca ggtgcgacca
      481 ggccggcggc acctcgccgc gatcgagctg ctcggcatgc cgcagccagc gctggccgaa
      541 tgcgcgcagc gcgtcgcgcg gcgtggcgag ctcgtgcgcc gcgagcgcgt cggccggcag
      601 gtcgcccgcg atcgcccacc agccgatcca ctcgggccgg tgcgcgctct cgacggccca
      661 gaggctcgcg tacggcatcg cgaaccaggc cggccgctcg ccgacgcggc catgcgcggg
      721 cgcgaggcgc gccatgcagg ccgcgacctc ggcggcgcgt tcgtcgcacc agcgttcgtc
      781 ttcgtttgct tccacgcgaa tgacctcaag ggcgccgcgg cggcagcggg atgaactcgg
      841 tctcgcccgg cacgtgctcg aagccagcgg cgcgtcccga ggtgaaggcc tcccagtcgt
      901 cctgcgcctg tgcgatgcgt tccttgcgcg acgagacgaa gttccagacg atgaagcgcg
      961 gcccgagcgg cgcgccgccg accagcacga tgcggccgcc gggcggcgcg ctcagcaccg
     1021 gctcgctgcc gggttcgagc acgaccatcg tgaacgccgg cacgggcgtg ccgtcgagcg
     1081 tgaagtcgtg gtcgacactg tagagcgcgc gctcctgcgc cgcgtccggg atcgtgagcg
     1141 acgcgccggc cgcgaagctc aggtcgaggt acagcgtcgg cgactttgtg gcgaccggcg
     1201 agcgcaggcc gaacgcgctg ccgatcagca cgcgcacccg cacgccattc acgtcgacgg
     1261 ccgggatctc ggcgcccggc acgtgctgaa agcccggcgc gtcttcttcg tgctcggccg
     1321 gcaccgcgag ccagagttgc aggccgtggc tgcggcgctc gaccgcgcgc agatcctcgg
     1381 gcgtgcgttc cgagtgcacg atgccgcggc ccgccgtcat caggttgatc gcgccgggct
     1441 cgatgcgctg caccacgccg gtcgagtcgc ggtgcatcat cgcgccctcg aacaggtagc
     1501 tgaccgtggc cagcccgatg tgcggatgcg ggcgcacatc gaagttgtcg cccggcagtg
     1561 ccgtgaccgg gccgaagtgg tcgaagaaca cgaacgggcc gacggcctgg cgccgcggcg
     1621 acggcagcga acggcggacc atgaagccgc cgcccaggtc cttctcgtgg ggcggcaggg
     1681 tttcgagcgc gggcatgggt cactccgaca ggcagttcgg cgacagtatc ggcgaccgcg
     1741 ggtcggtcgc ctaaaatccg cccttcgccc gcagtccctg ccgggccatc cagaaagacc
     1801 tccatgagtt tgaagtgcgg catcgtgggc ctgcccaacg tcggcaagtc caccctgttc
     1861 aatgccctca ccaaggccgg catcgcggcc gagaactacc cgttctgcac gatcgagccg
     1921 aacgtcggca tcgtcgagct gcccgacccg cggctgcagg acctggccgc gatcgtcgaa
     1981 cccgagcgca tcgtgccggc gatcgtcgag ttcgtcgaca tcgccgggct ggtggccggc
     2041 gcgtccaagg gcgaaggcct gggcaaccag ttcctcgcgc acatccgcga gaccgacgcg
     2101 atcgtcaacg tggtgcgctg cttcgatgac gagaacgtga tccacgtcgc cggcaaggtc
     2161 gacccgatct cggacatcga ggtgatccag accgaactct gcctggccga catgggcacg
     2221 gtggaaaaag gcctgcaccg ctacaacaag gtcgcgcgct cgggcgacaa ggaagcgcaa
     2281 aagatcgtcg ccgtgctcga gaagtgcatg gccgcgctga accagggcaa gccggtgcgc
     2341 acgatcgcgt tcggcaagga agacctggcg ctggtcaagc cgctgtgcct gatcaccgcg
     2401 aagccggcga tgttcgtcgg caacgtcgac gagaacggct tcgagaacaa cccctatctc
     2461 gacaagctgc gcgcgtatgc gcaggggcaa ggcgcgccgg tcgtcgcgat atgcgccaaa
     2521 accgaggccg aactggctga gatggacgac gcggaccggc tgatgttcct ctcggaaatg
     2581 ggccagaccg agccgggcct gaaccgcctg atccgcgccg cctacacgct gctgggcctg
     2641 cagacctact tcaccgccgg agtcaaggag gtacgcgcct ggacgatcca cgtcggcgac
     2701 accgcgccgc aggcggccgg cgtaatccac accgatttcg agcgcggctt catccgcgcc
     2761 cagacgatcg ctttcgccga cttcgtcgcg ttcaagggcg aacagggcgc gaaggacgcg
     2821 ggcaagatgc gcgccgaagg caaggactac gtcgtgaagg atggcgacgt gctgaacttc
     2881 ctgttcaacg tgtgacgcgg gtcggcaact gcaacttgtt tcgttgaatt gtcgaccgcg
     2941 gggctgcggc agcaccgcaa gccgcgcaga atcggccctt gacgaacccg gcgccgccgg
     3001 gccgcatcca ccccgagacg cgcgcgacgc ttggccaccg acacgcctac cgacaagccc
     3061 ttgcactgga cggccgcgtt cgcgctgacc gcgctgtgct acgcgctcgc cggcatcggc
     3121 gcgctgccgc tggcgattcc gccgggctat tcgacgccgc tctaccccgc cgccggcgtc
     3181 gcgctggcga gcgtgctgct gttcggcccg cgcatgctgc cggccgtcgc gctcggcgcg
     3241 ctgagcgtga acctctcgct cgcgttgcaa cgccacgcct tcagcccggc gctgctgccg
     3301 gtgccgctcg cgatcgccgt cggcgccgcc ctgcaggccc tcgccggcgc gctgctggcg
     3361 aagcgcttcg tgcggcagcc gctgacgctg tcggagccgc gcgacatcgc cgtcttcctg
     3421 ctcacggcca tggtctcgtg cctggtcagc ccggtcatcg gaaacctcgc gctgtggaca
     3481 gccggcagcg tgccgacggc cgagctgccg ttcagcgcct cgacctggtg gatcggcgac
     3541 ctgctcggcg tgctgatcgc cacgccgatc atcctgaccc tgttcggccg gccgcgcgac
     3601 gcctgggtgc cgcggcgcct gagcgtcggg ctgacgctcg cgctggtcac gctgctgctg
     3661 gccctgggca tccgccaggt cgcgcagtgg aacaacgagc gcgtgcagac cgcgttcgac
     3721 catgacgcgt cgaacgcggc gcaggcgctg gccgcccagt tgcgcgagcc gctgcaggcg
     3781 ctcgaggcgc tgaacggcgt gttcctcgcc gccgagcacg tcagcgccga ggcgatgcgc
     3841 ctggccacgc gcagctggct cgcgcccggc cgcctgcagg cgatgacctg gacggaacgt
     3901 gtggcgcgcg gcgacctgcc ggcgttcgag gcccgggtgc gcgtcgagcc cggcatggag
     3961 cgtttcaagg tcttcgaccg ccaggacgcc gcgggcgcgt cgccgcgcgc gggcgcgccg
     4021 gacgacgaca tgctggtggt gcgctacatc gagccgcccg ccggcaacac cggcgcgatc
     4081 ggcgtcaacg cgatgtcggt cgcggccgca cgcggcgcga tcgagcgcac gcgcgacacc
     4141 ggcgcgcccg cagccagcgg cggcttccgc ctgacgcagc aggccgcgca cgagagccag
     4201 ctcggcatcg tggtctacca ggccgtctac gcgccgcgcg cgccggcgcc ggcgcagcgt
     4261 gcggcggcgc tgcgcggcac cgtctcggtg gtgctgcgca tggacgacca gctgcaggcg
     4321 ctcgccgcgc agctgccgcc ctacctcgcg ctgtgcctgc tcgacgtgag cccgggcgag
     4381 gcgccgcgcc gcctcgccgg cgcgccgggc tgcgagacgc gccaggcacc gctgcagctg
     4441 gcgcgcgcgc tgccctacgc cggccgccgg tgggaggtgc gggtcacgcc ggcgccgggc
     4501 atgcagccga acgttcacag cgccgacgtg tggctgttcg cgctggtcgg cctgggctcg
     4561 accgcgatgc tcggcggctt cttgctcacc gtgaccggcc gcacccggcg catcgagacc
     4621 gccgtgcgcg agcgcacggc cgcgctgcag gccgaagtgc gcgag
//
LOCUS       DASRDG010000557         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1118815, whole genome shotgun sequence.
ACCESSION   DASRDG010000557 DASRDG010000000
VERSION     DASRDG010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118815"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFA35_09340"
     CDS             complement(<1..467)
                     /locus_tag="VFA35_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56417.1"
                     /translation="MPLLTPNGRPNPAFIHRAPTRLLTPAQEIVTEVVAEVMARGVEN
                     LERHWLQEALLEFETAIALMPEFPHAHWNRAQVLLMLGRYDEGFVEFDWRFKLFGDTL
                     WHQGVPVWCGEKLNGRRLLLWHEHGYGDTILLLRYLPVLLAMGAPLTVAVPEP"
     gene            complement(512..2101)
                     /locus_tag="VFA35_09345"
     CDS             complement(512..2101)
                     /locus_tag="VFA35_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004541751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HZT56418.1"
                     /translation="MTAVTPSLPQKRGRGRPRKVVLSRAETNIKWIEEWCRVPDGRRD
                     LIGKPVVLREWQRAAIRTIYDNPHGTRRAILSFGRKNGKTAFAAMLMLLHLCGPEAQV
                     NSQLFSAAQARAQAALLFEAAAKIVRMNPQLDINKDGFISINDTNKVMRCLELGTVYR
                     ALSAEVTTAFGLSPSFIVHDELGQVRGPRSPLYQALETATGAQEKPLSIVISTQAPTD
                     GDLLSLLIDDARSGHDPSTVLVLHTAPMECDPFADETIKMANPAYGDFLNAAEVRSMA
                     DAARRMPSAEADYRNLILNQRVATQQAFVPVSVWDECAAVPMPLDELEVYGGLDLSAV
                     SDLTALVLIGKHEGIWHVHPTFWLPGHGLREKSQEDRQPYDLWARDGHLITAAGKSVD
                     YEEVALYLREVFNRYKIMQIGFDDWAFAQFQPWLLKAGFGESEITAHFANFRQGSKSM
                     TPALRVLESELKNGRIAHGNHPVLRMCAMNAVTHGNDNARKLVKPHERGRIDGMVALA
                     MAMGVVPTPVGDTIDVDAMIV"
     gene            complement(2046..2462)
                     /locus_tag="VFA35_09350"
     CDS             complement(2046..2462)
                     /locus_tag="VFA35_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56419.1"
                     /translation="MTQRGRKSAKLLTVIGPHGVEPVRRPEPPASLNVECADEWRAVV
                     GAMSADYFRPETLALLEQRCRHVVWARRLAQLIEGEELKGEQFDERRYSGLLRVAAVQ
                     SHAIAALDTKLRLTQESVYDRSDPKPAAKKRPWQTA"
BASE COUNT          429 a          906 c          809 g          466 t
ORIGIN      
        1 aatggctcgg gcaccgctac cgtcaacggc gcacccatcg ccagcagtac cggcaggtag
       61 cgcagcagca ggatcgtgtc gccgtagcca tgctcgtgcc acagcaacaa ccgccggccg
      121 ttcagttttt cgccgcacca caccggcacg ccctgatgcc acagcgtgtc gccaaacaat
      181 ttgaagcgcc agtcgaactc gacgaagccc tcgtcgtagc ggccgagcat cagcagcacc
      241 tgcgcccggt tccagtgcgc atgcggaaac tcaggcatca gtgcaatagc ggtctcgaac
      301 tcgagcagcg cctcctgcag ccagtgccgc tcgaggttct ccacccctcg cgccatgacc
      361 tcagccacca cctcggtgac gatctcctgg gccggcgtca gtagccgagt tggcgcgcga
      421 tgtatgaacg ctggattcgg tcggccgttc ggcgtcaaca ggggcaccgg gtcatcgcga
      481 aactgccgca gccgccgttc tgataaccgc atcagacaat catcgcgtcg acgtcgatcg
      541 tgtcgccgac cggcgtcggc accacgccca tcgccatggc cagcgccacc atgccgtcaa
      601 tgcgcccgcg ctcgtgcggc ttgaccagct tgcgcgcgtt gtcgttgccg tgcgtcaccg
      661 cgttcatcgc gcacatgcgc agcaccgggt ggttgccgtg cgcgatgcgg ccgttcttca
      721 gctccgactc caacacccgc agcgcaggcg tcatcgactt gctgccctgg cggaagttgg
      781 cgaagtgcgc ggtgatctcg ctctcgccga agccggcttt cagcagccag ggctggaatt
      841 gtgcgaacgc ccagtcgtcg aagccgatct gcatgatctt gtagcggttg aacacctcgc
      901 gcagataaag cgccacctcc tcgtagtcga cgctcttgcc ggcggccgtg atcaggtgac
      961 cgtcgcgcgc ccacagatcg tacggctggc ggtcctcttg cgacttctcg cgcagcccat
     1021 gacccggcaa ccagaatgtc gggtgcacgt gccagatgcc ctcgtgcttg ccgatcagca
     1081 ccagcgccgt gagatcgctg accgccgata ggtcgagccc gccgtacacc tccagctcgt
     1141 ccagcggcat cggcacggcc gcgcattcgt cccacaccga gaccggaacg aacgcctgct
     1201 gcgtcgcgac tctctggttc aggatcaggt tgcggtagtc ggcttcggcc gacggcatgc
     1261 gccgggcggc gtcggccatc gagcgcacct cggcggcgtt gaggaagtcg ccgtaggccg
     1321 ggttcgccat cttgatggtc tcgtcggcga acggatcgca ttccattggc gcagtgtgca
     1381 gcaccagcac ggtgctcgga tcgtgtccac tgcgcgcatc gtcgatcagc agcgagagca
     1441 gatcgccgtc ggtcggcgcc tgcgtgctga tcacgatcga cagcggtttc tcttgcgcgc
     1501 cagtggccgt ctccagggcc tgataaagcg gcgagcgcgg accgcgaacc tggcccagct
     1561 catcatgcac gatgaagctc ggcgacaagc cgaacgccgt ggtcacctca gccgacagcg
     1621 cacgatagac cgtgcccaac tccaagcagc gcatcacctt gttggtgtcg ttgatgctga
     1681 tgaacccgtc cttgttgatg tcgagctgcg ggttcatgcg cacgatcttc gccgcggcct
     1741 cgaacagcag tgccgcctgt gcgcgcgcct gcgccgcgct gaacaactgg ctgttcacct
     1801 gcgcttccgg cccgcagagg tggagcagca tcagcatcgc ggcgaaggcc gtcttgccgt
     1861 tcttgcgccc gaacgacagg atcgctcggc gtgtgccgtg agggttgtcg taaatcgtgc
     1921 ggatcgcagc gcgctgccac tcgcgcagca ccaccggctt gccgatcaga tcacgccggc
     1981 cgtccggtac gcgacaccac tcctcgatcc atttgatgtt ggtctcggcg cgactgagaa
     2041 caactttacg cggtctgcca cggcctcttt tttgcggcag gcttggggtc actgcggtca
     2101 tacacactct cttgggttag tcggagcttg gtatcgagcg cggcaatcgc gtgtgattgc
     2161 accgccgcca cgcgcagcag cccgctgtag cggcgctcgt cgaactgctc gcccttcagc
     2221 tcctcgcctt cgatcagttg cgccaagcgc cgcgcccaca cgacgtgccg gcagcgctgc
     2281 tcgagcaggg caagcgtctc aggccggaag tagtcggccg acatcgcgcc cacgacggcg
     2341 cgccactcgt ccgcgcactc gacgttcagg ctggccggcg gctccggccg acgcaccggc
     2401 tcgacaccgt gcggcccgat cacggtcaat agcttcgccg acttgcgccc gcgttgtgtc
     2461 atgtcagggc cctttgggtt ggcggcgcgg gcccccgtgt gggtggtgca tcaacagggc
     2521 ttgggaggca ccgatgcacc gggctggact gagcccgcgc catctattgg tccgccgcga
     2581 tcacctcgaa catgcggtcg aagcagacaa
//
LOCUS       DASRDG010000558         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_199946, whole genome shotgun sequence.
ACCESSION   DASRDG010000558 DASRDG010000000
VERSION     DASRDG010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199946"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFA35_09355"
     CDS             complement(<1..780)
                     /locus_tag="VFA35_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56420.1"
                     /translation="MREGMRRWLIRGAWATGLCCGAMLLGLRLGRPIWYSDGAREHSA
                     SELAAAGMLQWPTPEAEVELPGPVQGRVAMLPDGKLIYGRTQADGTTDLVVFDPSRPG
                     LAPEPAYGLDTPSHELAPAVAADGTVYFASDRPGGSGGFDLYRSHWQNGGFTPPEPLP
                     LCNTARDETDPAPAPGTDELLFVRVDPNVRGGDHGVLLRAHLNGDTDPEPVFADVAAR
                     RPEAQPVDRDPVFARDGGAVWFVRREPGRPAALLRASRLGDA"
     gene            complement(815..1231)
                     /locus_tag="VFA35_09360"
     CDS             complement(815..1231)
                     /locus_tag="VFA35_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZT56421.1"
                     /translation="MRLDLDDTDDEFAMNLTPMLDIVFNLLLFFLAATTFAREEVAMD
                     LRLPQARSGAESKGGHQLIVNVFADGRLQVDGRVVTLEALRQKLQAAAARDKDQAVLV
                     RGDKQAQFGIGLQVLDACRLAKIGKVDFAALPEQKQ"
     gene            complement(1221..1916)
                     /locus_tag="VFA35_09365"
     CDS             complement(1221..1916)
                     /locus_tag="VFA35_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZT56422.1"
                     /translation="MLCMSFVPQEPSHVIATIGEVFAKGGFMMWPILGCSVLLVGLTI
                     ERLLSMRQRRVIPRAVALAVEQIRQGRADAVEPQILESMAPAARVLAAGVRRRGYPLG
                     DIERGMSDQVAREGALLRSNVRGVTLMAAVGPLCGLLGTVIGIARAFAAVEGVGQNRA
                     EMLASGIGVALYTTIFGLSVAIPATLIGAWLQGKVRRLMLAIDTAVSPAIEHLAPAPK
                     PPVPGEAEATHAA"
     gene            complement(1978..>2567)
                     /locus_tag="VFA35_09370"
     CDS             complement(1978..>2567)
                     /locus_tag="VFA35_09370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZT56423.1"
                     /translation="AEAGFLVGECCLKLGDCAHAAPQFRALLQQAPQHERAAAARLAL
                     GECDIELGNADEAVAALEEFLRARPADKTEAARAQLLLGRARLLRKEHDRAEAAFAKV
                     TELSEGALGAEAQFRLGESRAMRGDLEGAADAFVKLPILYAQEEWVRRGLLQAGLCYE
                     RLQQPAKARRFFDELVRRFPQSQEGKEAQSHLRGV"
BASE COUNT          340 a          984 c          902 g          341 t
ORIGIN      
        1 cgcgtcgccg agccggctcg cgcgcagcag ggccgccgga cggcccggct ccctgcgcac
       61 gaaccagacc gcgccgccgt cgcgcgcgaa caccgggtcg cggtcgaccg gctgcgcctc
      121 gggacgccgc gccgcgacgt ccgcgaacac gggctccgga tcggtgtcgc cgttcaggtg
      181 cgcgcggagc agcacgccgt ggtcgccgcc gcgcacgttc ggatcgacgc gcacgaacag
      241 cagctcgtcg gtgcccggcg ccggcgccgg atcggtctcg tcgcgcgccg tgttgcagag
      301 gggcagcggc tccggcggcg tgaagccgcc gttctgccag tgcgaacggt agagatcgaa
      361 cccgccgctg ccgcccggcc ggtcggaggc gaagtagacg gtgccgtccg cggcgaccgc
      421 gggcgcgagc tcgtggctcg gcgtgtcgag cccgtacgcc ggctccggtg cgaggcctgg
      481 ccgcgacgga tcgaacacca cgaggtcggt cgtgccgtcc gcctgcgtgc gcccgtagat
      541 cagcttgccg tcgggcagca tcgcgacgcg tccctgcacc ggcccgggca gctcgacttc
      601 ggcctcgggc gtcggccact gcagcatgcc cgccgccgcg agttcggacg ccgagtgctc
      661 gcgcgcgcca tcgctgtacc agatcggccg cccgaggcgc agcccgagca gcatcgcgcc
      721 gcagcagaga ccggtcgccc atgcgccccg gatcagccat cgccgcatgc cttcgcgcat
      781 cgccggtcct cctttcggcc tacggtcctc gccgctactg cttctgctcg ggcagggccg
      841 cgaagtcgac cttgccgatc ttcgccagcc ggcacgcgtc cagcacctgc agaccgatcc
      901 cgaactgcgc ctgcttgtcg ccgcgcacga gcacggcctg atccttgtcg cgcgcggccg
      961 cggcctgcag cttctgccgc agggcctcga gcgtgacgac ccggccgtcg acctgcagcc
     1021 ggccgtcggc gaacacgttg acgatcagct ggtggccgcc cttgctctcg gcgccgctgc
     1081 gcgcctgcgg caggcgcagg tccatcgcca cctcctcgcg cgcgaacgtc gtcgcggcga
     1141 ggaagaacag cagcaggttg aagacgatgt cgagcatcgg cgtcaggttc atcgcgaact
     1201 cgtcgtccgt gtcgtcgagg tcaagccgca tgggtcgcct ccgcctcgcc cggcaccggc
     1261 ggtttcggcg ccggcgccag gtgctcgatc gccggcgaga ccgccgtgtc gatcgccagc
     1321 atcagccgcc gcaccttgcc ctgcagccag gcgccgatca gcgtggcggg gatcgccacg
     1381 gacagcccga agatcgtcgt gtagagcgcg acgccgatgc ccgacgccag catctcggcg
     1441 cggttctgcc cgacgccctc gaccgccgcg aacgcgcgcg cgatgccgat cacggtgccg
     1501 agcaggccgc acagcggacc gaccgccgcc atcagcgtca cgccgcgcac gttgctgcgc
     1561 agcagcgcgc cttcgcgcgc gacctggtcg ctcatgccgc gctcgatgtc gccgagcgga
     1621 tagccgcgcc ggcgcacacc cgccgccagt acgcgcgccg cgggcgccat cgactcgagg
     1681 atctgcggct cgacggcgtc ggcgcgaccc tgccggatct gctcgaccgc gagcgccacc
     1741 gcgcgcggga tcacgcgccg ctggcgcatg ctcagcagcc gctcgatcgt gagcccgacc
     1801 agcagcacgg agcagccgag gatcggccac atcatgaacc cgcctttggc gaacacctcg
     1861 ccgatcgtcg cgatcacgtg cgacggttcc tgcgggacga aggacatgca cagcatcgaa
     1921 tcgactcctc tggatggcgg gtcggatggc gtatcggatg gcggatcagc gggacggcta
     1981 gacgccgcgc aggtgcgact gcgcttcctt gccctcctgg ctctgcggga agcggcgcac
     2041 gagttcgtcg aagaaacgcc gggccttggc gggctgctgc aggcgctcgt agcacaggcc
     2101 ggcctgcagc aggccgcggc gcacccactc ctcctgcgcg tagaggatcg gcagcttgac
     2161 gaacgcgtcg gcggcaccct cgaggtcgcc gcgcatggcg cggctctcgc cgagccggaa
     2221 ctgcgcctcg gcgcccagcg cgccttcgga cagctcggtg accttcgcga acgcggcctc
     2281 ggcgcggtcg tgctccttgc gcaacagacg cgcgcgcccg agcagcagct gcgcgcgcgc
     2341 cgcctcggtc ttgtcggcgg gcctggcgcg caggaactcc tccagtgccg cgaccgcctc
     2401 gtcggcgttg ccgagctcga tgtcgcactc gccaagcgcg agccgcgccg cggcggcgcg
     2461 ttcgtgctgc ggcgcctgct gcagcagcgc gcggaactgc ggcgccgcgt gcgcgcagtc
     2521 gccgagcttc aggcagcact cgccgacgag gaagccggcc tcggccg
//
LOCUS       DASRDG010000559         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1119171, whole genome shotgun sequence.
ACCESSION   DASRDG010000559 DASRDG010000000
VERSION     DASRDG010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119171"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VFA35_09375"
     CDS             <1..712
                     /locus_tag="VFA35_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZT56424.1"
                     /translation="QRLRELAQHLQNSIEQERTAIAREIHDDVGGSLTALKFDLAWIA
                     RHSDSDDVLSRVNAALETVTLAIDSSKRIMHNLRPAILEQGLVAALHWLASRFEKRTG
                     IACEIRLPADAPDLPAGVPLVAYRTAQEALTNITKHAQASRVQLDLSLAGGVLSLEIS
                     DNGRGLSNEDLAKARSFGIRGLHERAGTVGGWVELSSSGNGTTMILSVPLNPPGIADI
                     PSAATHDDDDPSQWGDLL"
     gene            822..1460
                     /locus_tag="VFA35_09380"
     CDS             822..1460
                     /locus_tag="VFA35_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZT56425.1"
                     /translation="MIKVFLCDDHALIRRGIRDTLSDAPDIEVVGEAGDYGELRTLMR
                     EHSADVLVLDINLPGRSGLDVLHVLKDEGTPMRVLVVSMYPEDQYAIRALRAGAFGYV
                     NKGGDPAQIVAAVRTVAQGRKYVTPEIAQMLVESLTTPMIANAHEKLSDRELQTLVMI
                     ASGKRLSDIAEELMLSPKTVSVYRARVLEKLGLANNSEMTVYAIRNGLVGSE"
     gene            1607..>2031
                     /locus_tag="VFA35_09385"
     CDS             1607..>2031
                     /locus_tag="VFA35_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56426.1"
                     /translation="MSQPPALLMIASFAVGAALLWLSAELGVAAGMRSPRLARPLFAI
                     AVGLGAGALWWPANALRVPDVAVVSTQGFLWLSSWPACLLLAGLAGAAGALWNRGERA
                     WAMLATAGLPIAAYLHVVFGAPDWLGVGALTLALSAGLLL"
BASE COUNT          300 a          722 c          698 g          311 t
ORIGIN      
        1 gcagcgcctg cgcgaactcg cgcagcacct gcagaacagc atcgagcagg agcgcaccgc
       61 gatcgcacgc gagatccacg acgacgtggg cggctcgctc acggcgctga agttcgacct
      121 ggcgtggatc gcgcgccact ctgactccga cgacgtgctg agccgcgtca acgcggcgct
      181 cgaaaccgtc acgctggcga tcgattcgag caagcggatc atgcacaacc tgcgcccggc
      241 gatcctcgaa caggggctgg tcgccgcgct gcactggctc gcgagccgct tcgagaagcg
      301 caccggcatt gcgtgcgaga tccgcctgcc ggccgacgcg cccgacctgc ccgcgggcgt
      361 gccgctcgtg gcctaccgca ccgcgcagga ggcgctcacc aacatcacca agcacgcgca
      421 ggcgtcgcgc gtgcaactcg acctctcgct cgccggcggc gttctgtcgc tcgagatcag
      481 cgacaacggc cggggcttga gcaacgagga cctcgcgaag gcgcgctcgt tcggcatccg
      541 cggcctgcac gagcgtgccg gcacggtcgg cggctgggtc gaattgagca gcagtggcaa
      601 cggcacgacg atgatcctgt cggtgccctt gaatccgccg ggcatcgctg atataccctc
      661 cgcagcaacg cacgacgacg acgatccgag ccagtggggt gatctgttgt gatgacgacc
      721 cctcgcccgc cgagcacagc gttcgatccc ccgacgggat gcgggccggc ttgggagcgg
      781 cccggcgctc ggcccgcctg atcgcgaagg cagcacaaac gatgatcaag gtctttctct
      841 gcgacgacca cgcgctgatc cggcgcggca tccgcgacac gctctcggac gcgcccgaca
      901 tcgaggtcgt cggcgaggcc ggcgactacg gcgagttgcg caccttgatg cgcgagcaca
      961 gcgccgatgt gctcgtgctc gacatcaacc tgcccggccg cagcggcctg gacgtgctgc
     1021 acgtgctgaa ggacgaaggc acgccgatgc gcgtgctggt ggtttcgatg tacccggaag
     1081 accagtacgc gatccgcgcg ctgcgcgccg gcgcgttcgg ctacgtcaac aagggcggcg
     1141 acccggcgca gatcgtcgcc gcggtgcgca ccgtggccca gggccgcaag tacgtgacgc
     1201 ccgagatcgc gcagatgctg gtcgagagcc tgaccacgcc gatgatcgcg aacgcgcacg
     1261 agaagctctc cgaccgcgag ctgcagacgc tggtgatgat cgcctcgggc aagcgcctct
     1321 cggacatcgc cgaagagctg atgctcagcc cgaagaccgt cagcgtctac cgcgcccgcg
     1381 tgctcgaaaa gctcggcctg gccaacaact ccgagatgac ggtctacgcg atccgcaacg
     1441 ggctggtcgg cagcgaatag cgccgcatca cgtcggttcg gcgccgcgcc ctgcggcatg
     1501 cgtcacgcta gtcgcggcat tggcgcccgc gccgcgcgcg ttgctgccga aaacccgcac
     1561 catccgctct gcttgtcgcg ccgagcccgg cgctgtccgg agttcgatga gtcagccgcc
     1621 tgccctgttg atgatcgcca gcttcgctgt cggtgctgcg ctgctctggt tgtcggctga
     1681 actcggcgtc gcagccggca tgcgctcgcc gcgactggcc cggccgctgt tcgcgatcgc
     1741 cgtcgggctc ggtgccggtg cgctgtggtg gccggcgaac gcgctgcgcg tgcccgacgt
     1801 ggcggttgtg agcacgcaag gcttcctgtg gctgtcgtcg tggcccgcct gcttgctgct
     1861 cgcgggcctg gccggcgccg cgggtgcgct ctggaaccgc ggcgagcgcg cctgggccat
     1921 gctcgccacg gccggcttgc cgatcgccgc ctacctgcat gtggtgttcg gcgcgcccga
     1981 ttggctcggc gtcggcgcgc tgacgctggc gctgtcggcc ggcctcttgc t
//
LOCUS       DASRDG010000560         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1119346, whole genome shotgun sequence.
ACCESSION   DASRDG010000560 DASRDG010000000
VERSION     DASRDG010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119346"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFA35_09390"
     CDS             complement(<1..713)
                     /locus_tag="VFA35_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZT56427.1"
                     /translation="MIVGTAGHIDHGKTTLVRALTGVNTDRLPEEQRRGISIELGYAY
                     LDLPDRADSPGARPPEAGQPRRGADGVSGRGLHRIGFIDVPGHERLVHTMLAGATGID
                     LALLLVAADDGVMPQTREHLAVLSLLGVARGAVVVTKIDRVDAQRVATVRAEAAALVA
                     GSTLAAAPVLAVSAVTGQGVDALRAWLFDAAAQIARRDDASLGFRLAVDRAFTLAGVG
                     TVVTGTAHAGSVQVDDELLL"
     gene            complement(720..1577)
                     /gene="fdhD"
                     /locus_tag="VFA35_09395"
     CDS             complement(720..1577)
                     /gene="fdhD"
                     /locus_tag="VFA35_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZT56428.1"
                     /translation="MTNSAPILSTSRSSDDASGGPAGLATVDAIALRDGVPTPRSECV
                     ADEVAIAIEVNGISHAVMLATPLDLEDFALGFLLSEGLIKEPGDLLDVEPEVTPQGVT
                     LGLTVTQRCLWGFKERRRTLTGRTGCGLCGTESIDQAMRPVAHVDAGALRVPAAALSR
                     AMRELAEGQELQRACGAVHAAAWATAEGQVKLVREDVGRHNALDKLIGALVHAGVDAH
                     QGFIVVTSRASYEMVQKTASAGVGLLAAISAPTRLAIQTAQTAGVTLAGFVRGTNAVL
                     YAHAERVVS"
     gene            complement(1578..2522)
                     /gene="fdhE"
                     /locus_tag="VFA35_09400"
     CDS             complement(1578..2522)
                     /gene="fdhE"
                     /locus_tag="VFA35_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HZT56429.1"
                     /translation="MQRILQPGEIEALDRVAFPRVRLPQPAVLFQERAARLAQLAEGS
                     PIADYLRFAARLVQGQHRQAAQARAPEPLAEAAVRRSQANSMPLWPASEALPPGWCDT
                     LRALLAEFSADPAVPELLHPVLERLAAIDDDALGVLARQALADNIGREDLAAAPLVMA
                     ALQVGFAVRAAALDVRSVPYTDPATVCPVCGSAPVASVLRIGGEAGGHRYLHCSLCAT
                     EWHMVRVKCSHCESTQGVRYQGVENADSKVSAQVVLAETCDACHTYRKLVNQEKDPYA
                     EPLVDDLASLTLDLLMGETEFSRASGNPLLAIEKPLAV"
     gene            complement(2644..3351)
                     /locus_tag="VFA35_09405"
     CDS             complement(2644..3351)
                     /locus_tag="VFA35_09405"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HZT56430.1"
                     /translation="MSHAEHSKDAEFAVDLRGRPAIQRYQPNDRTNHWLTAITFVMLA
                     LSGLALFHPAMFWLTALFGGGPWTRILHPFIGLVMFFSFMGLALRFWHHNFLDKADIE
                     WMTKIGDVLQNREDRLPKIGSYNAGQKMLFWSLLACMIGLLLTGIVMWRAYFSHFFHI
                     DIIRVSSLLHALCGFVLIAAIAVHIYAAIWIKGSIKAMTRGTVSYAWARRHHARWYDE
                     IVQGEKKTDRQHKAAGG"
     gene            complement(3338..>3793)
                     /locus_tag="VFA35_09410"
     CDS             complement(3338..>3793)
                     /locus_tag="VFA35_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta
                     transmembrane domain-containing protein"
                     /protein_id="HZT56431.1"
                     /translation="GLEPACVKTCPTGAIMFGTKQAMKDQAAERIEDLKERGFEKAGL
                     YDPAGVGGTHVMYVLHHADKPSLYHGLPDEPKISPMVSLWKGFAKPLALAGIALTALA
                     GFFHYTRIGPNETTDAEEADADAKALARREAAGVLPPAHHHEEAHHEPR"
BASE COUNT          567 a         1319 c         1354 g          553 t
ORIGIN      
        1 agcaagagct cgtcgtcgac ctgcacgctg ccggcatgcg cggtgccggt aaccacggtg
       61 ccgacgccgg cgagcgtgaa cgcgcggtcg acggcaaggc gaaagccgag cgacgcgtcg
      121 tcgcgccgcg cgatctgcgc cgcggcgtcg aagagccagg ccctgagcgc atcgacaccc
      181 tgccccgtga cggccgagac ggcgagcacc ggcgccgccg cgagcgtcga gccggcgacg
      241 agtgccgccg cctcggcgcg aaccgtcgct acgcgctgcg catcgacgcg atcgatcttg
      301 gtgacgacca ccgcgccgcg cgcgacgccc agcagcgaca gcaccgcgag gtgctcgcgc
      361 gtctgcggca tcacgccgtc gtcggccgcc acgagcagca aggcaaggtc gatgccggtc
      421 gcgccggcga gcatggtgtg caccaggcgc tcgtggccgg gcacgtcgat gaagccgatg
      481 cgatggagcc cccgcccgga tacaccgtcc gccccccgcc ggggctgtcc ggcttcgggc
      541 ggccgggcgc cggggctgtc ggcgcgatcg ggcaggtcga gataggcata gcccagctcg
      601 atgctgatgc cgcgccgttg ctcctccggc aggcggtcgg tgttcacgcc ggtcagcgcg
      661 cgcaccagcg tggtcttgcc gtggtcgatg tggcccgcgg tgccgacgat catgtggcgt
      721 cagctcacca cccgctcggc gtgcgcgtac aagaccgcgt tcgtgccgcg cacgaagccg
      781 gccagcgtga cacccgcggt ctgcgcggtc tggatcgcca gccgcgtcgg tgccgaaatt
      841 gcagccagca gcccgacacc ggcgctggcc gtcttctgca ccatctcgta gctcgcgcgg
      901 ctggtcacga cgatgaagcc ctggtgcgcg tcgacgcccg catgcacgag cgcgccgatc
      961 agcttgtcga gcgcgttgtg gcgacccacg tcttcacgca ccagcttcac ctgcccctcg
     1021 gccgtggccc aggccgcggc gtgcacggcg ccgcaggcgc gctgcagttc ctggccttcg
     1081 gcgagctcgc gcatcgcgcg cgacagcgca gcggccggca cgcgcaacgc gccggcgtcg
     1141 acgtgcgcca ccggccgcat cgcctggtcg atcgactcgg tgccgcacag cccgcagccg
     1201 gtgcgcccgg tcagcgtgcg acggcgctcc ttgaagcccc acaggcagcg ctgcgtgacc
     1261 gtcaacccca gcgtgacgcc ttgcggcgtg acctcgggct cgacgtcgag cagatcgccg
     1321 ggctccttga tcaggccctc gctgagcagg aagccgagcg cgaagtcctc gaggtcgagc
     1381 ggcgtcgcga gcatcaccgc gtgcgagata ccgttgacct cgatggcgat ggcaacttcg
     1441 tcggcgacgc actcgctgcg cggcgtcggc acgccgtcgc gcagcgcgat cgcgtcgacc
     1501 gtcgcgaggc cggccgggcc gcccgaagcg tcgtccgaac tgcgcgatgt ggagaggatg
     1561 ggagctgaat tcgtcatcta taccgccagc ggcttctcga tcgcgagcaa cgggttgccg
     1621 ctggcgcgcg aaaactcggt ctcgcccatc agcaggtcga gggtgaggct ggccaggtcg
     1681 tcgaccagcg gctcggcata ggggtccttt tcctggttca ccagcttgcg ataggtgtgg
     1741 caggcgtcgc aggtctcggc cagcacgacc tgggcgctga ccttgctgtc ggcattctcg
     1801 acgccctggt agcgcacgcc ctgggtcgat tcgcagtggc tgcacttcac gcgcaccatg
     1861 tgccattcgg tggcgcacag gctgcagtgc aggtagcggt gaccgccggc ctcgccgccg
     1921 atgcgcagca cgctggccac gggggcgctg ccgcataccg ggcagaccgt ggccgggtcg
     1981 gtgtagggca cggatcgcac atcgagcgcc gcggcgcgga ccgcgaagcc gacttgcaac
     2041 gcggccatca cgagcggcgc ggcggccagg tcctcgcggc cgatgttgtc ggccagggcc
     2101 tggcgcgcca gaacgccgag cgcgtcgtca tcgatggcgg cgaggcgctc gagcaccggg
     2161 tgcaacagct cgggcacggc cggatcggcc gagaactccg ccagcagcgc gcgcagcgtg
     2221 tcgcaccacc cgggcgggag cgcctccgag gccggccaca acggcatgct gttggcctgg
     2281 gaacggcgca ccgctgcctc ggccagcggc tcgggcgcgc gcgcctgagc cgcctggcgg
     2341 tgctgcccct gcaccagccg cgcggcgaaa cgcaggtagt cggcgatggg gctgccctcg
     2401 gccagctgcg cgaggcgtgc ggcgcgttcc tggaacagta ccgccggctg gggcagccgc
     2461 acgcgcggga acgccacacg gtcgagggct tcgatttcgc caggttggag gatgcgttgc
     2521 acgggaggaa gagaggtgat tgaaaagagg aaatgccggg acggacccgg cacctctgtc
     2581 tggacgcaag ccgatcgcgg cgagtctatg ccagcccgca ggggccggca cgggacaggg
     2641 accctatccg cccgcggcct tgtgctggcg atcggtcttc ttttcgccct ggacgatctc
     2701 gtcgtaccag cgcgcgtggt ggcggcgcgc ccacgcgtag ctgaccgtgc cccgcgtcat
     2761 ggccttgatc gagcccttga tccagatcgc cgcatagatg tggaccgcga tggcggcgat
     2821 gagtacgaag ccgcagagcg cgtgcagcag cgaggacacg cggatgatgt cgatgtggaa
     2881 gaagtggctg aagtacgcgc gccacatgac gatgccggtc agcagcaggc cgatcatgca
     2941 ggccagcaac gaccagaaca gcatcttctg cccggcgttg tagctgccga tcttgggcag
     3001 gcgatcctcg cggttctgca gcacgtcgcc gatcttcgtc atccactcga tgtcggcctt
     3061 gtccaggaag ttgtggtgcc agaagcgcag cgccagcccc atgaacgaga agaacatcac
     3121 cagcccgatg aagggatgca ggatgcgcgt ccacggcccg ccgccgaaca gcgcggtgag
     3181 ccagaacatc gccggatgga acagcgccag ccccgacagc gccagcatca cgaaggtgat
     3241 cgcggtcagc cagtggttgg tccggtcgtt gggctggtag cgctggatcg cgggccggcc
     3301 gcgcaggtcg accgcgaact cggcgtcctt cgaatgctca gcgtggctca tggtgcgcct
     3361 cctcgtgatg atgcgcgggc ggcaacacgc ccgccgcttc gcggcgcgcc agcgccttcg
     3421 cgtccgcgtc ggcctcctcc gcgtcggtgg tctcgttggg gccgatgcgc gtgtagtgga
     3481 agaagcctgc cagcgcagtc agcgcaatgc cggccagggc cagcggcttg gcgaagccct
     3541 tccacaggct gaccatcggg ctgatcttcg gctcgtcggg caggccgtgg tacagcgaag
     3601 gcttgtcggc gtggtgcagc acgtacatga cgtgcgtgcc gccgacgccg gccggatcgt
     3661 acaggcccgc cttctcgaag ccgcgctcct tcaggtcctc gatgcgctct gccgcctggt
     3721 ccttcatggc ctgcttggtg ccgaacatga tggcgccggt cgggcaggtc ttcacgcagg
     3781 ccggctccag gcc
//
LOCUS       DASRDG010000561         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1012443, whole genome shotgun sequence.
ACCESSION   DASRDG010000561 DASRDG010000000
VERSION     DASRDG010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012443"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFA35_09415"
     CDS             <1..803
                     /locus_tag="VFA35_09415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZT56432.1"
                     /translation="LLNRENELKAEQLRRRDLQQRLWWLLAAAFVLAFAVVALLYRRV
                     RRTNRLLSTSNELLKVQSERDPLTGLANRRHFQTAMRRLAEDGKLCGTVYLIDIDHFK
                     HINDRHGHGAGDAVLVEVARRLRETLREQDLIVRWGGEEFLVVVQALGADQVDALAQR
                     MLCVLDRAPVGAGAQRVAVTASIGFATFPIGPGLLRVPWERAIDLVDTAMYLAKAHGR
                     NRAYGVRLLQARDEAALDAITHSLESAWRDGQVGLTLLQGHAALAVAA"
     gene            800..>2463
                     /locus_tag="VFA35_09420"
     CDS             800..>2463
                     /locus_tag="VFA35_09420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZT56433.1"
                     /translation="MRFLVGALLAALFGGAAAAPAHAPHAPDPFAEQVDALVNAGFEH
                     PARALLALRTMPAARIGSTDEQRVLLQAIGEVEAQSDGAARAGAIAEQLLALAPDDAS
                     GRAVAASNLVRALVAENAGQPDVAAALAQSALPAFASGCPDAPASAAAGPECDYRSAW
                     RALAVLERRAGSQGLPASAAAHARSALALAESAGDARRSAVNLSSLALYASGRGEREL
                     AQRLVAQARKLAAQTGDPAEQARVRNTEARLASAQGDRSGALRRYDEALALAVQAQAP
                     RLEALLLNNLSDAYARLHRPADALRAAERALAIVQRHHDLRAERVLINNAGIAKIGLG
                     RIAEGKHDLARLLELWQRSGETGRQVATLLEFGEALAAAGDARGALDLYHRERALSAE
                     LMRANRAIALKELQVRNDADARQRDIELLARDNALKTTALANRDLLQRIGWLLAAVMA
                     LAIGLVTMLYRRVRETHQKLAASHLQLQAQSERDPLTNLANRRHFQAVMTALCGAGGF
                     EGALLLVDIDHFKHVNDAQGHAAGDQVLVEVARRLNAAVRSDDLVVR"
BASE COUNT          328 a          909 c          932 g          294 t
ORIGIN      
        1 tgctgctgaa ccgcgagaac gagctcaagg ccgagcagct gcgccggcgc gacctgcagc
       61 agcggctgtg gtggctgctg gcggcggcgt tcgtgctcgc gttcgcggtg gtcgcgctgc
      121 tgtaccgccg cgtgcgccgg acgaaccggc tgctctcgac cagcaacgaa ctgctgaagg
      181 tgcaaagcga gcgcgacccg ctcaccggcc tggcgaaccg ccgccacttc cagaccgcga
      241 tgcggcgcct ggccgaggac ggcaagctct gcggcaccgt ctacctgatc gacatcgacc
      301 acttcaagca catcaacgac cgccacggcc atggcgccgg cgatgcggtg ctggtcgagg
      361 tggcgcggcg tctgcgcgag acgctgcgcg agcaggacct gatcgtgcgc tggggcggcg
      421 aggagtttct ggtggtcgtg caggcgctcg gcgccgatca ggtcgacgcg ctcgcgcagc
      481 gcatgctttg cgtgctcgac cgcgcgccgg tcggcgccgg cgcgcagcgc gtcgcggtga
      541 ccgcgtcgat cggcttcgcg accttcccga tcggaccggg cctgctgcgc gtgccgtggg
      601 agcgcgcgat cgacctggtc gacaccgcga tgtacctggc caaggcgcac ggccgcaacc
      661 gcgcctacgg cgtgcggctg ctgcaggccc gcgacgaggc cgcgctcgac gcgatcacgc
      721 actcgctcga atcggcctgg cgcgacgggc aggtcgggct cacgctgctg caagggcacg
      781 cggcgctggc ggtggcggca tgaggttcct cgtcggtgcc ttgctcgccg cgctgttcgg
      841 cggcgcggcg gccgccccgg cgcatgcgcc gcacgcgccc gacccgttcg ccgagcaggt
      901 cgacgcgctg gtgaacgcgg gcttcgagca cccggcgcgg gcgctgctcg cgctgcgcac
      961 gatgccggcc gcgcgtatcg gctcgaccga cgagcagcgc gtgctgctgc aggcgatcgg
     1021 cgaggtcgag gcgcagtccg acggcgccgc gcgcgccggt gccatcgccg agcagttgct
     1081 ggcgctcgcg cccgacgacg cgagcggccg cgcggtcgcc gcatcgaacc tggtgcgcgc
     1141 gctcgtcgcc gagaacgccg ggcagcccga tgtcgcggcg gcgctggcgc aatcggcgct
     1201 gccggcgttt gccagcggtt gccccgacgc gccagcaagc gcggccgcgg gccccgaatg
     1261 cgactaccgc tcggcctggc gtgcgctggc ggtcctcgag cggcgcgccg ggagccaggg
     1321 gctgccggcc agcgcggcgg cgcatgcgcg cagcgcgctg gcgctggccg agtcggccgg
     1381 cgacgcgcgc cgcagcgccg tcaacctgag ttcgctcgcg ctgtacgcga gcgggcgcgg
     1441 cgagcgcgag ctcgcgcagc gcctggtcgc gcaggcacgc aagctcgcgg cgcagaccgg
     1501 cgacccggcc gagcaggcgc gcgtgcgcaa caccgaagcg cgcctggcca gcgcccaggg
     1561 cgaccggtcc ggcgcgctgc gccgctacga cgaggcgctc gcgctggcgg tgcaggccca
     1621 ggcgccgcgg ctcgaggcac tgctgctgaa caacctgagc gacgcctatg cgcggctgca
     1681 ccgcccggcc gacgcgctgc gcgctgccga gcgcgcgctg gcgatcgtgc agcgccacca
     1741 cgacctgcgc gccgagcgcg tgctgatcaa caacgccggc atcgcgaaga tcggactggg
     1801 ccgcatcgcc gagggcaagc acgacctcgc gcgcctgctc gagctctggc agcgcagcgg
     1861 cgagaccggc cggcaggtgg ccacgctgct cgagttcggc gaggcgctgg ccgccgccgg
     1921 cgacgcccgc ggcgcactcg atctctacca ccgcgagcgc gcgctgagcg ccgagctgat
     1981 gcgcgcgaac cgcgcgatcg cgctgaagga gctgcaagtg cgcaacgacg ccgacgcgcg
     2041 ccagcgcgac atcgagctgc tcgcgcgcga caacgcgctg aagaccaccg cactcgcgaa
     2101 ccgcgacctg ctgcaacgca tcggctggct gctcgccgcg gtgatggcgc tggcgatcgg
     2161 cctggtgacg atgctgtatc gccgcgtgcg cgagacgcac cagaagctgg ccgccagcca
     2221 cctgcagctg caggcgcaga gcgagcgcga cccgctgacg aacctggcga accggcgcca
     2281 cttccaggcc gtgatgacgg cgctgtgcgg cgccggcggc ttcgagggcg cgctgctgct
     2341 ggtcgacatc gaccacttca agcatgtcaa cgacgcgcag ggccacgccg ccggcgacca
     2401 ggtgctggtc gaggtggcgc ggcgcctgaa cgcggccgtg cgcagcgacg acctggtcgt
     2461 gcg
//
LOCUS       DASRDG010000562         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1347642, whole genome shotgun sequence.
ACCESSION   DASRDG010000562 DASRDG010000000
VERSION     DASRDG010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347642"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFA35_09425"
     CDS             <1..303
                     /locus_tag="VFA35_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HZT56434.1"
                     /translation="GPFKPLLAALHRLQLAGNAQMRIRTALVTARSAPAHERAIRTLM
                     KWNVHVDEAMFLGGLPKGEFLREFEPDFFFDDQTGHVDSAAAHVPAGHVASGVSNG"
     gene            complement(313..1083)
                     /locus_tag="VFA35_09430"
     CDS             complement(313..1083)
                     /locus_tag="VFA35_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZT56435.1"
                     /translation="MNPSPNAAAPAPAATPAPIHQRARAPHAAELADIPWLAALDREE
                     RAVAVADLKVVQVAAGELLCRVGRPVTFWFGVIDGFLKMSNDSHRGHARGPSGDQSMP
                     ITFTGLPPGGWFGEGTVIKREAYRYNIQAVRTSVVAGLSVATFHWLLERSIPFNRFII
                     QQLNERLGQFIGARETDRMIDVDARVARSLGALFHPVLYPGVGGLLRITQQELGYLIG
                     LSRQRVNEALNSLQAKGLIRIEYGGVSVLDLEGLRRIG"
     gene            1202..>2582
                     /locus_tag="VFA35_09435"
     CDS             1202..>2582
                     /locus_tag="VFA35_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZT56436.1"
                     /translation="MPTTFPRLMLDHAARRPNAAALREKEFGIWQTLSWSALATLVRA
                     LACGLHEAGLKRGEHLVVIGENRPRLYASMLAAQVLGAIPVPLYQDAATPEFVFPIIN
                     AEVRFAIVEDQEQVDKMLELRAQCPQLKHIWFDDPRGLRHYAEPGLSPLDALIKAGHA
                     FNAVHPGVFEAEVHQTRPDDVAAMFFTSGTTGNPKGVVHTHATLIDRARAGAAFDKLT
                     HDEEVLAYLPPAWIGQNIFSYAQWLACGYVVNCPESPSTVGIDLNEIGPTYYFAPPRI
                     FEGLLTTVMIRMEDAGAIKRWLFRHLMDVARRVGPRLMDGLAVSALERLHYALGKLVI
                     YGPLRNTLGLSRVRVAYTAGEAIGPDLFTFYRSIGINLKQLYGSTETAVFVCLQPDHE
                     AKADTVGVPIDGVQIRLTDAGEILVKSPGLLREYYKNPTATAEVLTSEGWYHTGDAGF
                     LDAGGHLKII"
BASE COUNT          413 a          920 c          865 g          384 t
ORIGIN      
        1 ggcccgttca agccgctgct ggccgcgctg catcggctgc agctggccgg caacgcgcag
       61 atgcgcatcc gcaccgcgct ggtcacggca cgcagcgcgc cggcgcacga gcgcgccatc
      121 cgcacgctga tgaagtggaa cgtccacgtg gacgaggcca tgttcctcgg cggcctgccc
      181 aagggcgagt tcctgcgcga gttcgagccc gacttcttct tcgacgacca gaccggccac
      241 gtcgattcgg ccgccgccca cgtgccggcc gggcacgtgg cctcgggcgt gagcaacggc
      301 tgacgcggtg gctcagccga tccggcgcag cccctcgaga tcgagcacgc tgacgccgcc
      361 gtactcgatc cggatcagcc ccttggcctg cagcgagttc agcgcctcat tgacgcgctg
      421 ccgcgacaag ccgatcaggt agccgagctc ctgctgcgtg atgcgcagca ggccgccgac
      481 gcccgggtaa agcaccgggt ggaacagcgc gccgaggctg cgcgcgacgc gggcgtcgac
      541 gtcgatcatc cggtcggtct cgcgcgcacc gatgaactgg ccgagccgct cgttcagctg
      601 ctggatgatg aagcggttga acgggatgct gcgctcgagc agccagtgga aggtcgcgac
      661 gctcagcccg gcaaccacgc tggtgcgcac cgcctggatg ttgtagcggt aggcctcgcg
      721 cttgatcacc gtgccttcgc cgaaccagcc gccgggcggc aggccggtga aggtgatcgg
      781 catgctctgg tcccccgagg ggcctctggc gtggcccctg tggctgtcgt tgctcatctt
      841 caggaagccg tcgatcacgc cgaaccagaa ggtcaccggg cggccgaccc ggcacagcag
      901 ctcgccggcc gcgacctgca ccaccttcag gtccgcaacc gcgacggcac gctcctcgcg
      961 atcgagcgcg gccagccacg gaatgtcggc gagctcggcc gcgtgaggcg cacgcgcccg
     1021 ctggtggatc ggcgccggcg ttgccgcagg cgccggcgcg gcagcgttgg ggcttgggtt
     1081 catcgcggga aacacccgag gggactgtcc ctaggattgt cgtcagaacg acaacctggc
     1141 gtcaaacttg tttctacatt cgggctcaga cctgcctcgt tccccgaccc gaagaccccg
     1201 cgtgccaacc accttccccc gcctgatgct cgaccacgcg gcccggcgtc cgaacgccgc
     1261 ggccttgcgc gagaaggagt tcggcatctg gcagacgctg agctggagcg ccctggccac
     1321 cctggtgcgc gcgctcgcgt gcgggctgca cgaggccggg ctgaagcgcg gcgagcacct
     1381 cgtcgtgatc ggcgagaacc gcccgcggct gtatgcatcg atgctggccg cacaggtgct
     1441 cggggcgatc cccgtgccgc tgtaccagga cgcggcgacg cccgagttcg tgttcccgat
     1501 catcaatgcc gaggtcaggt tcgcgatcgt cgaagaccag gagcaggtcg acaagatgct
     1561 cgagctgcgc gcgcagtgcc cgcaactaaa gcacatctgg ttcgacgacc cgcgcgggct
     1621 gcgccactac gcggagcccg gcctctcacc gctcgatgcg ctgatcaagg cggggcatgc
     1681 attcaacgcg gtgcacccag gcgtgttcga ggccgaggtg caccagacgc ggcccgacga
     1741 cgtggccgcg atgttcttca cctcgggcac gaccggcaac ccgaagggcg tggtccacac
     1801 ccacgccacg ctgatcgacc gcgcacgcgc cggcgcggcc ttcgacaagc tcacgcacga
     1861 cgaagaggtg ctcgcctacc tgccacccgc gtggatcggc cagaacatct tctcgtacgc
     1921 gcagtggctg gcctgtggct acgtcgtcaa ctgccccgag tcgccgagca cggtcggcat
     1981 cgacctgaac gagatcggcc cgacctacta cttcgcgccg ccgcgcatct tcgagggcct
     2041 gctgacgacg gtgatgatcc gcatggagga cgccggtgcg atcaagcgct ggctgttccg
     2101 tcacctcatg gacgtggcgc ggcgcgtcgg cccgaggctg atggacggcc tggcggtgag
     2161 cgcgctcgag cgcctgcact acgcgctcgg caagctcgtc atctacggcc cgctgcgcaa
     2221 cacgctcggc ctctcgcgcg tgcgcgtcgc ctacaccgcg ggcgaggcga tcggcccgga
     2281 cctgttcacc ttctaccgct cgatcggcat caacctgaag cagctctacg gctcgaccga
     2341 gaccgcagtg ttcgtctgcc tgcagcccga ccacgaagcg aaggcggaca cggtcggcgt
     2401 gccgatcgac ggcgtgcaga tcaggctcac cgatgccggc gagatcctcg tcaagtcgcc
     2461 cggcctgctg cgcgagtact acaagaaccc gacggcaacc gccgaggtgc tgacgagcga
     2521 aggctggtac cacacgggcg acgccggttt cctcgatgcc ggcgggcacc tgaagatcat
     2581 cg
//
LOCUS       DASRDG010000563         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_658830, whole genome shotgun sequence.
ACCESSION   DASRDG010000563 DASRDG010000000
VERSION     DASRDG010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658830"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFA35_09440"
     CDS             complement(<1..542)
                     /locus_tag="VFA35_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56437.1"
                     /translation="MLQRFERSEAPTHGLQRGAAFAEPGAGLVFLAGELLVPGLALVE
                     HRGEGFDLLAMFVVVGGELFRRRAQRGEIGGLLFRAQRFEAAADFHRLAIEIVEARAF
                     DFGGLARIGAGLGMLVPLRLPLRERGLGGVLCFGSLLGGFAQRFELRLAVEQRGAQRL
                     DLLAIGRDMLAEFGIGIFGF"
     gene            complement(549..809)
                     /locus_tag="VFA35_09445"
     CDS             complement(549..809)
                     /locus_tag="VFA35_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56438.1"
                     /translation="MSGQRTRARVATLLRRNQRDIGLARLREFAEEIERHIDEHAVEQ
                     AREHGFDRGFPTGFDVERFTEARRLVEAEAFEPFVRRRFLLT"
     gene            complement(822..1772)
                     /locus_tag="VFA35_09450"
     CDS             complement(822..1772)
                     /locus_tag="VFA35_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56439.1"
                     /translation="MLGETLFRQRFARLLQQVFDAVALLLVAAEFVIARFQHLACLDE
                     LLVDEQALIEVDLALGFEVGQRRLSRFKLLRDLGLARFELGELAAHAQQRLFKRIDAC
                     AFGFYVEREPVRLLRCLARIHARFLACFEQATAFAIEMLAMLFLVAHARDGFFEPRAC
                     FARSDITGGELGFEFGDFAVDARYARLRGFELAAVALQSARKLGHAAVRDVEFALRVV
                     ARAFGIGALRLDFVKARVERRDAFLGGGEFVLERVDFLLACDHARLRVAAPAHAREAR
                     AEPFAGRRNDGFVRREAAGARTRRIKRIGGVNAVEDMQRG"
     gene            1782..>2007
                     /locus_tag="VFA35_09455"
     CDS             1782..>2007
                     /locus_tag="VFA35_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56440.1"
                     /translation="MRAQQLQDAIVAAGHEFAALIESLPQGADHEEWQRDITELDAKI
                     RRLEPVNLAAISEYEEQSQRKTYLDSQLTDL"
BASE COUNT          413 a          698 c          644 g          252 t
ORIGIN      
        1 tcgaaaccga aaatgcctat gccgaactcg gcgagcatat cgcggccgat cgcgagcaga
       61 tcgagacgct gcgcacctcg ctgctcgacg gcgagccgaa gctcgaaacg ctgcgcgaag
      121 ccgccgagca gactgccgaa gcacagcacg ccgccgagtc cgcgctcgcg caatggcagt
      181 cgcagtggga cgagcattcc aagtccagcg ccgattcggg ccaggccgcc gaagtcgaac
      241 gcacgcgcct cgactatctc gatcgccagt cgatggaagt cggccgccgc ctcgaagcgc
      301 tgcgcgcgga aaagcaggcc gccgatttcg ccacgctgag cgcggcggcg gaacaactcg
      361 ccgccgacca cgacgaacat cgcgagaagg tcgaaacctt caccgcgatg ctcgacgagc
      421 gcaaggccag gcacgagcaa ctcgccggcg aggaacacca ggccagcgcc tggctcagcg
      481 aaggccgcac ctcgctgcag accatgcgtg ggcgcctcgc ttcgctcgaa gcgctgcagc
      541 atgccgcatt aggtcaggag aaatcgccgg cgcacgaatg gctcgaacgc ctcggcctcg
      601 acaaggcgcc gcgcctcggt gaagcgctcg acgtcgaagc cggttgggaa accgcggtcg
      661 aaaccgtgct ctcgggcctg ttcgacggcg tgctcgtcga tgtgccgttc gatttcctcg
      721 gcgaactcgc gcaggcgagc aaggccgata tcgcgctggt ttcggcgcag caaggtggca
      781 acgcgcgcgc gggtacgctg gccgctcacg tgcaaggacc atcagccgcg ctgcatatcc
      841 tcgacagcat ttacaccgcc gattcgcttg atgcggcgcg tgcgcgcgcc agcagcctcg
      901 cgccgcacga atccgtcatt acgccggccg gcgaatggct cggcgcgggc ttcgcgcgcg
      961 tgcgccggcg cggcgacgcg caggcgggcg tgatcgcacg cgagaaggaa atccacgcgc
     1021 tcaaggacga actcgccgcc tccgaggaac gcatcgcgac gctcaacgcg cgctttgacg
     1081 aaatcaagtc gcagcgcgcc gatgccgaac gcacgcgcga cgacgcgcag cgcgaactct
     1141 acgtcgcgca ccgccgcgtg gccgagcttg cgggcgcttt gcagagccac cgcggcaagc
     1201 tcgaaaccgc gcaggcgcgc atagcgcgcg tcgacggcga aatcgccgaa ctcgaaaccc
     1261 agctcgccac cggtgatgtc actacgcgcg aagcacgcgc gcggctcgaa gaagccgtct
     1321 cgcgcatggg cgaccaggaa cagcatcgcc agcatctcga tggcgaacgc cgtcgcctgc
     1381 tcgaagcacg cgaggaagcg cgcatgaatg cgcgcgaggc atcggagcag acgcaccggc
     1441 tcgcgctcga catagaatcc aaacgcacag gcgtcaatgc gcttgaacag gcgctgttgc
     1501 gcatgagcgg ccagctcgcc cagctcgaat cgcgcaagac cgagatcgcg cagcagcttg
     1561 aatcgggaca gacgccgctg gccgacctcg aatccgagcg ccagatctac ctcgatcagc
     1621 gcctgctcat cgacaagcaa ctcgtcgagg cacgcaaggt gctggaaacg cgcgatcacg
     1681 aattccgcgg ccacgagcag gagcgccacc gcatcgaaca cctgctgcag caggcgcgcg
     1741 aagcgctgtc ggaaaagcgt ctcgccgagc acggcctgaa gatgcgtgcg cagcagttgc
     1801 aggacgcgat cgtcgccgcg ggtcatgagt tcgccgcgct gatcgagtcg ctgccgcagg
     1861 gcgccgacca cgaggaatgg cagcgcgaca tcaccgaact cgacgccaag atcaggcgcc
     1921 tcgaaccggt caatctcgcc gcgatctccg aatacgagga gcagtcgcag cgcaagacct
     1981 atctcgactc gcaactcacc gacctca
//
LOCUS       DASRDG010000564         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_541826, whole genome shotgun sequence.
ACCESSION   DASRDG010000564 DASRDG010000000
VERSION     DASRDG010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541826"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFA35_09460"
     CDS             <1..200
                     /locus_tag="VFA35_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HZT56441.1"
                     /translation="VRAVLITGQPLGPALERAIAALRAFDAHAGALQPHFAYGALDKP
                     AYTRAHLMHLANHWNEAADTA"
     gene            230..988
                     /locus_tag="VFA35_09465"
     CDS             230..988
                     /locus_tag="VFA35_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZT56442.1"
                     /translation="MSIKTATLNSVATIEIARPEKKNAITGAMYLQMAAALDAAKADA
                     GVRAVLITGQPGIFTSGNDIEDFVQRPAGEPPAFVFMRALIGCDKPVIAAVTGAAIGI
                     GTTLLLHCDFVYVSDEARLAMPFVSLGVVPEFASSLIVPALMGNVRAAEKLLLGEPFS
                     GVDAVECGIANAVLPAGEVVNHARRIAERFNALPPGAVRDTKALMRGPRAKLVAEAIA
                     TEAEVFAKRLRSPEAQEAFSAFFQKRKPDFSNFS"
     gene            1007..1729
                     /locus_tag="VFA35_09470"
     CDS             1007..1729
                     /locus_tag="VFA35_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZT56443.1"
                     /translation="MSLALKLVLSPVLLAQARAARRRAPALPEAAGPREGQLGSGTPA
                     LRVLIAGDSSAAGVGVLNQDHAFAGHFTRTLHRRSGRAVRWRLRAKSGLTTQQVHALL
                     RADPPPAADIAVVLTGVNDVIGLVSPQRALQHRAALAEWLLGEGRARYVLFAPLPPIN
                     QFPLLPQPLRHVMGSEARLHDEAMAAWATTRHNVFHAPIALQLAPAAMSRDGFHPGEP
                     VYRACGETLARFVVDKILPLEG"
     gene            2004..2780
                     /gene="otsB"
                     /locus_tag="VFA35_09475"
     CDS             2004..2780
                     /gene="otsB"
                     /locus_tag="VFA35_09475"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HZT56444.1"
                     /translation="MTPSDPPALAASAALFLDFDGTLAPIAPRPQDVRVPPWVPAALQ
                     ALARQLDGALAIVSGRPLAQLDALLAPLRLPAAGAHGAEWRGASGATTRRLDAPPPPL
                     VQAARALVAAHAGLILETKPAGFALHYRARPELEDTCREALFAALSARPEAGAVWQWL
                     DGHCVFELKQRSVSKGLAVRALLAQAPFAGRQPVFVGDDVTDEDGIAAVQAAGGFGVR
                     VGAGASQARYRLADTDAVGAWLMSAASVAALRQDPEKNDA"
     gene            2777..>3171
                     /locus_tag="VFA35_09480"
     CDS             2777..>3171
                     /locus_tag="VFA35_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HZT56445.1"
                     /translation="MMNNQASLNLGLIGNCTISALIDAQARMVWCCMPRFDSDPVFHA
                     LLDSAEGIGEEGSFQIVLDRCVRTEQAYDENTAILRTRLFDDHDNAVEVTDFAPRFTH
                     HERSFHPMQLVRRLKPVQGSPRIRIVLRPR"
BASE COUNT          416 a         1174 c         1139 g          442 t
ORIGIN      
        1 gcgtgcgcgc ggtgctgatc accggccagc cgctggggcc cgcgctcgag cgtgcgatcg
       61 ccgcgctgcg cgcgttcgat gcgcacgccg gcgcgctcca acctcacttt gcctatggcg
      121 cgctcgacaa gccggcctac acgcgcgcgc acctgatgca cctggccaac cactggaacg
      181 aagccgccga cacggcatga atcacccacc gcatgaagga gaccacagca tgagcatcaa
      241 gaccgccacc ctgaacagcg tcgcgacgat cgagatcgcg cgccccgaga agaagaacgc
      301 gatcaccggc gcaatgtacc tgcagatggc cgccgcgctc gacgcggcca aggccgacgc
      361 cggcgtgcgc gcggtgctga tcaccggcca gcccggcatc ttcacctcgg gcaacgacat
      421 cgaggacttc gtgcagcgcc cggcgggcga gccgcccgcg ttcgtcttca tgcgcgcgct
      481 gatcggctgc gacaagccgg tgatcgcggc cgtcaccggc gcggcgatcg gtatcggcac
      541 gacgctgctg ctgcactgcg acttcgtcta cgtgtccgac gaggcgcgcc tggcgatgcc
      601 cttcgtcagc ctcggcgtgg tgcccgagtt cgcgtcgagc ctgatcgtgc cggcgctgat
      661 gggcaacgtg cgcgcggccg agaagctgct gctcggcgag ccgttcagcg gcgtcgatgc
      721 ggtcgagtgc ggcatcgcga atgcggtgct gccggccggc gaggtcgtga accatgcgcg
      781 ccgcatcgcc gagcgcttca acgcgctgcc gcccggcgcg gtgcgcgaca ccaaggcgct
      841 gatgcgcggc cctcgcgcga agctcgtcgc cgaggcgatc gcgaccgagg ccgaggtgtt
      901 cgccaagcgg ctgcgcagcc ccgaagcgca agaggcgttc agcgcgttct tccagaagcg
      961 caagcccgac ttctcgaact tctcgtgacc ttcccatgat ccgggcatgt cgcttgcgct
     1021 gaagctggtc ctctcgccgg tgctgctcgc gcaggcccgg gcggcgcgcc ggcgcgcgcc
     1081 cgcgctgccc gaggccgccg gcccgcgcga gggccagctc ggcagcggca cgcccgcact
     1141 gcgcgtgctg atcgcgggcg actcgtcggc cgccggcgtc ggggtgctga accaggacca
     1201 cgcgttcgcc ggccacttca cgcgcacgct gcaccgccgc agcggccgcg cggtgcgctg
     1261 gcggctgcgc gcgaagtcgg gcctgacgac gcagcaggtg cacgcgctgc tgcgcgccga
     1321 tccgccgccg gcggccgaca tcgcggtcgt gctgaccggc gtgaacgacg tgatcggcct
     1381 cgtctcgccg cagcgcgcgc tgcagcaccg cgcagcgctg gccgagtggc tgctcggcga
     1441 gggccgcgcg cgctacgtgc tgttcgcgcc gctgccgccg atcaaccagt ttcctctgct
     1501 gccgcaaccc ttgcgccatg tgatgggcag cgaagcgcgc ttgcacgacg aggcgatggc
     1561 ggcctgggcg acgacccggc acaacgtctt ccacgcgccg atcgcgctgc agctcgcgcc
     1621 ggcggcgatg tcgcgcgacg gctttcaccc gggcgagccg gtctaccgcg cctgcggcga
     1681 aacgctcgcc cgcttcgtcg tcgacaagat ccttccgctg gaggggtgac gacacctggc
     1741 gggccggcgc ggcgttaccg aacgcccgaa tttctgtcgg acggcgccta cagcgtgccc
     1801 cggacagtgt cttcggcgac ggcctccggt ggtgcgcgca cgccgccgcc gaccatggcc
     1861 aacgccgggt gacgcgggct gtggcggggt tcggcgccga cttttccgtt ggctgaattg
     1921 cgactttcgc gcgctcgggc gtcgcgccgc cagcggttcg ccgcgctccg tgcgggtgca
     1981 tgagtctgct agggttgccc tcgatgacac caagcgaccc ccctgcgttg gcggcgtcgg
     2041 cggcgctgtt cctcgacttc gacggcacgc tggcaccgat cgcaccgcgc cctcaggacg
     2101 tgcgggtgcc gccgtgggtg ccggccgcgc tgcaggcgct ggcgcgccaa ctcgatggcg
     2161 cgctggcgat cgtcagcggc cgcccgctcg cgcagctcga cgcgcttctg gcaccgctgc
     2221 gcctgcccgc cgccggcgcg cacggggccg agtggcgcgg cgcgtcgggc gccaccacgc
     2281 gccggctcga cgcgccgccg cccccgctcg tgcaggccgc gcgcgcgctc gtggcggcgc
     2341 atgcgggcct gatcctcgag accaagccgg ctggcttcgc gctgcactac cgcgcgcggc
     2401 ccgagctcga agacacatgc cgcgaggcgc tgttcgcggc gctctccgcg cggcccgagg
     2461 cgggcgccgt ctggcagtgg ctggacgggc actgcgtgtt cgaactgaag cagcgcagcg
     2521 tctcgaaagg cctggcggtg cgcgcgctgc tggcgcaggc gccgttcgcc gggcgccagc
     2581 cggtgttcgt cggcgacgac gtgaccgacg aggacggcat cgcggcggtg caggcggccg
     2641 gcgggttcgg cgtgcgcgtc ggcgcgggtg cgtcgcaggc gcgctaccgg ctggccgaca
     2701 ccgacgcggt cggtgcgtgg ctgatgtccg cggcgtccgt ggcggcgctg cgccaagacc
     2761 cggagaagaa cgacgcatga tgaacaacca agccagcctg aacctcggcc tgatcggcaa
     2821 ctgcacgatc agcgcgctga tcgacgcgca ggcgcgcatg gtctggtgct gcatgccgcg
     2881 cttcgacagc gacccggtgt tccacgcgct gctcgactcg gccgagggca tcggcgagga
     2941 gggcagcttc cagatcgtgc tcgaccgctg cgtgcgcacc gagcaggcct acgacgagaa
     3001 caccgcgatc ctgcgcaccc ggctcttcga cgaccacgac aacgcggtcg aggtcaccga
     3061 cttcgcgccg cgcttcacgc accacgagcg ctcgttccac ccgatgcagc tggtgcggcg
     3121 cctgaagccg gtgcaggggt cgccgcgcat ccgcatcgtg ctgcgcccgc g
//
LOCUS       DASRDG010000565         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_658919, whole genome shotgun sequence.
ACCESSION   DASRDG010000565 DASRDG010000000
VERSION     DASRDG010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658919"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..588
                     /locus_tag="VFA35_09485"
     CDS             124..588
                     /locus_tag="VFA35_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZT56446.1"
                     /translation="MMEIRDLYRDVILDHNRHPRNFGRLDPADASAVGHNPLCGDALS
                     LTLRFDGDRVSELRFNGQGCAISVASASLMTEAVAGKTRPEVESLYQEVHSLLTDANH
                     KPAVSLGKLMALAGVREFPARVKCASLCWHTLHAAMHPGADGQHASVSTEQP"
     gene            585..1139
                     /gene="sufT"
                     /locus_tag="VFA35_09490"
     CDS             585..1139
                     /gene="sufT"
                     /locus_tag="VFA35_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HZT56447.1"
                     /translation="MSGHPSEPVTLSRDVEAVIVPAGTPITLKAGLSGFITQALGGSF
                     TLYIEGNLYRIPGAQADAIGKEIVQAPELPPGATEDDVRELAWTQMRSCYDPEIPVNI
                     VDLGLVYACAVTPLGDGRRKISVSMTLTAPGCGMGEVLVRDVREKLELIPTVAEADVQ
                     LVFDPPWSQSMMSDAAKLQTGMMW"
     gene            1142..1606
                     /locus_tag="VFA35_09495"
     CDS             1142..1606
                     /locus_tag="VFA35_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZT56448.1"
                     /translation="MTELILMRHAAAVQAAIGGGDYERELSAPGRVEAVQAARELARA
                     APPIERILYSPAQRTSATARIVAAEQSLGPQAIVAVPSLYTATPAAISAAIAREHGGA
                     SVLLVVGHNPGVSDFGGELSRQRGHLPTAGYWRVRLDYEEWHALLRAGGAKG"
     gene            1603..>2069
                     /locus_tag="VFA35_09500"
     CDS             1603..>2069
                     /locus_tag="VFA35_09500"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZT56449.1"
                     /translation="MSSPHPALFLGHGSPMHAIEPSRYSAAWTELGRALPPPRAILAI
                     SAHWYTRGTAVTAQPQPRTIHDFHGFPPELYRLQYPASGDPQLAARVAQLLSPAPVRL
                     DLDWGLDHGSWAVLRYLYPSADVPIVQLSVDGTVGPQAHFELAQRLAPLRDERV"
BASE COUNT          323 a          728 c          708 g          310 t
ORIGIN      
        1 catgccggtc atgacgtact acgggctcgc cgcgacggtg cgcgcctcct tcggctgcta
       61 cagcaccagc gacgacatcg agcggctggc gctagggctt gcgcgcgtgc gcgaggtgtt
      121 cgcgtgatgg agatccgcga cctgtatcgc gacgtgatac tcgaccacaa ccgccatccg
      181 cggaatttcg gccgcctcga cccggcggac gcctcggccg tgggccacaa cccgttgtgt
      241 ggcgatgccc tgagccttac gctgcgtttc gacggcgatc gcgtcagcga actgcgcttc
      301 aacggccagg gttgcgccat ctcggtggct tcggcctcgc tcatgaccga ggccgtggcg
      361 ggcaagacac gccccgaggt cgagtcgctc taccaggaag tgcactcgct gctgacggac
      421 gcaaaccaca agccggccgt atcgctcggc aagctcatgg cgcttgcggg agtgcgcgag
      481 ttcccggccc gcgtcaagtg cgcgagcctt tgctggcaca cgctccatgc ggcaatgcat
      541 ccaggcgcgg acggccagca cgcgagcgtt tcgacggagc agccgtgagc ggtcacccga
      601 gcgaaccggt caccttgagc cgcgacgtcg aggccgtgat cgtgcccgcg ggcacgccaa
      661 tcacgctcaa ggccggcctc agcggcttca tcacgcaggc gctcggcggc agcttcacgc
      721 tctacatcga gggcaacctg tatcgcattc ccggcgcgca ggccgacgcc atcggcaagg
      781 agatcgtgca ggcgccggag ttgccgcccg gcgcgaccga ggacgacgtg cgggagctcg
      841 cgtggacgca gatgcgcagc tgctacgacc cggagattcc agtcaacatc gtcgacttgg
      901 ggctggtata cgcttgcgcc gtcacgcctc tgggcgatgg ccggcgcaag atcagcgtga
      961 gcatgacgct gacggcgccc ggctgcggca tgggcgaggt gctcgtgcgt gacgtgcgcg
     1021 agaagctcga gctgatcccg accgtggccg aggccgacgt gcaactcgtg ttcgacccgc
     1081 cgtggagcca atcgatgatg tcggatgcgg ccaagctcca gaccggcatg atgtggtagc
     1141 gatgaccgag ctcatcctca tgcggcacgc ggccgcggtc caggccgcca ttggcggcgg
     1201 cgactacgag cgcgaactgt ccgcaccggg gcgcgtcgaa gcggtgcagg cggcccgcga
     1261 gctcgcacgc gcggcgcctc cgatcgagcg gatcctctat agcccggcgc agcgcacgag
     1321 cgcgaccgcc aggatcgtcg cggcggagca gtcgctcggc ccgcaagcga tcgtggcggt
     1381 accgtcactg tacacagcca ccccggccgc gatcagtgcg gcgatcgcgc gcgaacacgg
     1441 cggcgcaagc gtgctgctcg tcgtcggcca caatcccggc gtcagcgact tcggcggcga
     1501 gctctcgcgc cagcgcggcc acctgccgac agcaggatac tggcgggtca ggctcgatta
     1561 cgaggagtgg cacgcgctgc tgcgcgccgg cggcgcgaaa ggatgagctc cccgcacccg
     1621 gccctgttcc tcggccacgg ctcgccgatg cacgcaatcg agccttcgcg ttattcggct
     1681 gcgtggaccg aactcgggcg cgcgctgccg cctccgcgcg cgatcctcgc catttcagcc
     1741 cactggtaca cgcgcggcac ggcggtcacg gcgcagccgc aaccccgcac gatccacgac
     1801 ttccacgggt ttccgcccga gctgtaccga ttgcagtacc cggcaagcgg cgatccgcag
     1861 ctggcagcgc gcgtggcgca gttgctctcg ccggcgccgg tgcggctcga tctcgactgg
     1921 ggcctggacc acggcagctg ggcggtgctg cgctatctct atccatcggc cgacgtgcca
     1981 atcgtgcagc tgtccgtgga cggcaccgtc ggcccgcagg cgcatttcga actggcacag
     2041 cggcttgcac cgctgcgcga cgaacgcgt
//
LOCUS       DASRDG010000566         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1013020, whole genome shotgun sequence.
ACCESSION   DASRDG010000566 DASRDG010000000
VERSION     DASRDG010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013020"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VFA35_09505"
     CDS             complement(<1..1194)
                     /locus_tag="VFA35_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZT56450.1"
                     /translation="MDTPVPQTLDTTRRTLIKGVAAAGAASLLPGLNAVVYAQGSDKP
                     EKEEVRIGFIPLTDCASVVMASVLGFDKKYGIKIVPTKEASWAGVRDKLVNGELDMAH
                     VLYGLVYGVHLGIGGPKKDMAVLMTLNNNGQAITLSKKLADKGAVDGPSLAKLMSGDK
                     REYTFAQTFPTGTHAMWLYYWLASAGINPLKDAKVITVPPPQMVANMRVGNMDGYCVG
                     EPWGHRAIADGIGITAITTQDIWKDHPEKVLGTTNDFVKKYPNTARAVVMAVLEAGKW
                     IDASLGNKNKMAETVADKSYVNTSVDVINQRILGRYQNGLGKTWDDPNYMKFYNDGAV
                     TFPYLSDGMWFLTQHKRWGLLKEHPDYLAVATQINQIGLYKQAAAQVKVPVPKDVLRT
                     SKLVDG"
     gene            complement(1405..2031)
                     /locus_tag="VFA35_09510"
     CDS             complement(1405..2031)
                     /locus_tag="VFA35_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZT56451.1"
                     /translation="MTTPAILRIVIVAPDSLVADHPAAETEVRRSRWLRIGLLEGGYN
                     IVAVLPADVFLPERLAQIGPDMIIVDAESQARDTLEHVVMATRDARRPIVLFTEDNDT
                     SHVQQAIAAGVTAYVVAGLAPDRVKPVLEVALARFQHEEAMRQELAAARTELSGRKLI
                     DRAKGILMRSQGIAEDEAYARLRKTAMDKGMKLADVAQRVIDVADLLA"
BASE COUNT          333 a          709 c          770 g          417 t
ORIGIN      
        1 gccgtccacc agcttgctgg tgcgcaacac gtccttcggc accggcacct tgacctgcgc
       61 tgcggcctgc ttgtacaggc cgatctggtt gatctgcgtg gcgacggcca ggtagtcggg
      121 gtgctctttc agcagccccc agcgcttgtg ctgggtgagg aaccacatgc cgtcgctgag
      181 gtacgggaag gtgaccgcgc cgtcgttgta gaacttcatg tagttcgggt cgtcccaggt
      241 cttgcccaga ccgttctggt agcggccgag gatgcgctgg ttgatcacgt cgacgctggt
      301 gttgacgtag ctcttgtcgg ccaccgtctc ggccatcttg ttcttgttgc ccaggctggc
      361 gtcgatccac ttgccggctt cgagaaccgc catcacgacc gctcgcgcgg tgttcgggta
      421 cttcttgacg aagtcattcg tggtgccgag caccttttcg gggtgatcct tccagatgtc
      481 ttgcgtcgtg atcgcggtga tgccgatgcc gtcggcgatc gcgcggtggc cccagggttc
      541 gccgacgcag tagccgtcca tgttgcccac gcgcatgttc gcgaccatct gcggcggcgg
      601 cacggtgatg accttggcgt ccttcagcgg gttgatgccg gccgaggcga gccagtagta
      661 cagccacatc gcgtgcgtgc cggtcgggaa ggtctgcgca aaggtgtact cgcgcttgtc
      721 gccgctcatc agcttggcca gcgacgggcc atcgaccgcc cctttgtcgg ccagcttctt
      781 cgacagcgtg atcgcttggc cgttgttgtt cagggtcatc agcacggcca tgtccttctt
      841 ggggccgccg atgccgaggt gcacgccgta gaccaggccg tagagcacgt gcgccatgtc
      901 gagctcgccg ttgaccagct tgtcgcgcac gccggcccac gaggcttcct tggtcggcac
      961 gatcttgatg ccgtacttct tgtcgaagcc cagcaccgag gccatcacaa ccgaagcgca
     1021 atcggtcagc gggatgaagc cgatgcgtac ttcctctttc tcgggcttgt cggagccctg
     1081 cgcgtagacg acggcgttca gcccgggcag gaggctggca gcgcccgccg ccgcgacgcc
     1141 cttgatgagc gtgcggcggg tcgtgtcgag tgtttgggga acaggtgtgt ccatggggct
     1201 gtggtggcga tacggaaaac aaaaaggcgt ccgcacgggc ccggctcgaa gccaggattc
     1261 gtggggacgc cagcgtccgg cgcgaagctc accagccttg gtgcgctcgc gcggtgtcag
     1321 aagatcgcca ttgaccttcc gtggggcatt aagcaaccgg cgtgccacgc gcgggcgcac
     1381 gccgacggtg cgcgtccggc cgcgtcaggc gagcaggtcg gccacatcga tcacgcgctg
     1441 cgcgacgtcg gcaagcttca ttcccttgtc catcgcggtc ttgcgcagcc gcgcgtaggc
     1501 ctcgtcctcg gcgatcccct ggctgcgcat caggatgcct ttggcgcgat cgatcagctt
     1561 gcggcccgag agctcggtgc gcgccgcggc cagttcctga cgcatcgctt cttcgtgctg
     1621 aaagcgtgcg agcgccactt cgagcaccgg tttgacgcgg tcgggcgcca ggccggccac
     1681 cacgtaggcc gtcacgcccg cggcgatcgc ctgctgaaca tgcgaggtgt cgttgtcctc
     1741 ggtgaacagc acgatcggac ggcgcgcatc gcgcgtggcc atgacgacat gctcgagcgt
     1801 gtcgcgcgcc tggctttcgg cgtcgacgat gatcatgtcg gggccgatct gggccagccg
     1861 ttcgggcagg aagacgtcgg cgggcagcac cgcgacaatg ttgtagccgc cttcgagcag
     1921 accgatgcgc agccagcgcg agcggcgcac ttcggtttcg gcggcgggat ggtcggccac
     1981 cagcgagtcg ggcgcaacga tgacgatgcg caggatggcg ggcgtggtca tgccccgtgt
     2041 ggagcaacat tcacgccaat ggcgggcgga aacggatgcg gcaaggatca gcggatcagc
     2101 ggttggcgtg ggtcgcggca agcgcgcgtc gcagccagcc tccgaacggg cgcagcgagc
     2161 cgaacagcga atccgccgcc ggggcctcac gctcggcgac caggtggcgc gattcgagca
     2221 tctcgagga
//
LOCUS       DASRDG010000567         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1470820, whole genome shotgun sequence.
ACCESSION   DASRDG010000567 DASRDG010000000
VERSION     DASRDG010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470820"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /gene="nadB"
                     /locus_tag="VFA35_09515"
     CDS             <1..1342
                     /gene="nadB"
                     /locus_tag="VFA35_09515"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZT56452.1"
                     /translation="PLGLHLTREGGHGVRRIAHAADATGKAIHDALLARAAAHPNIEL
                     REQAFAVDLITSRHAKRAESPQRCYGVYALDIASGRVDALAASAVVLATGGIGKVYRY
                     TTNPDTATGDGIAMAWRAGCRVANMEFIQFHPTCLYHPQERSFLITEALRGEGAILRL
                     PDAPPGSPPSAGTRFMPAHDERAELAPRDVVARAIDFEMKKHGLDNVLLDATHLGEDF
                     LKSHFPTIHARCLQLGIDIARQAIPVVPAAHYTCGGIVTDLDGRCDLPGLYAVGEAGY
                     TGLHGANRLASNSLLECVVLGRTCAEHVLHAPRANAPALPAWDESQVEDADEQVVISH
                     NWDELRLLMWNYVGIVRTTRRLERALHRIELLRSEIEDYYANFRVNRDLLELRNLVDC
                     AELVVRSALLRHESRGLHYSRDFPQPLPVSFATVLTPPARRDANAQATPVTRIE"
     gene            complement(1312..2178)
                     /gene="nadC"
                     /locus_tag="VFA35_09520"
     CDS             complement(1312..2178)
                     /gene="nadC"
                     /locus_tag="VFA35_09520"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZT56453.1"
                     /translation="MFDTDETLAQARERNIRDALAEDIGRGDWTGQLIPAGRRVRARV
                     KVREHAVLCGRDWFDGCVRALDPAAWIAWQFDEGAEMPADSLVCEIEGDARALLAAER
                     PALNFLQLLSATATVTRAHVNAVKGASPNPRGCAVLDTRKTLPGLRQAQKYAVRVGGG
                     QNQRLALWHGILIKENHIAAAGGVAAALANAAALNSGVQIQIEVETLTQLREALAAGA
                     ASVLLDNFGEAAMREAVAITAGRALLEASGGLTLDQLRSVAATGVDRISVGKLTKDVR
                     AIDYSMRVTGVA"
     gene            complement(2185..>2653)
                     /gene="nadA"
                     /locus_tag="VFA35_09525"
     CDS             complement(2185..>2653)
                     /gene="nadA"
                     /locus_tag="VFA35_09525"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZT56454.1"
                     /translation="IELELLRREHPDAQVLVHPESPASVVAQADVVGSTTQLLKAVAD
                     GKAPTYIVATDNGIMHRMRQLAPGKLLLEAPTAGESATCKSCAHCPWMAMNGLPGVLA
                     CLEHGRGEISVPEPVRSRALGCIERMLDFVAAHPAAVAASGAQQGFVPNIGVA"
BASE COUNT          365 a         1000 c          917 g          371 t
ORIGIN      
        1 cccgctcgga ttgcacctca cgcgcgaggg cgggcatggc gtgcggcgca tcgcgcacgc
       61 ggccgatgcg accggcaagg cgatccacga tgcgctgctc gcgcgcgccg cggcgcaccc
      121 gaacatcgag ctgcgcgagc aggcttttgc cgtcgacctg atcacgtcgc ggcatgcaaa
      181 gcgggccgaa tcgccgcagc gctgctacgg cgtctacgcg ctcgacatcg cgagcggccg
      241 cgtcgatgcg ttggccgcga gcgccgtcgt gctcgccacc ggcggcatcg gcaaggtcta
      301 ccgctacacc accaaccccg ataccgccac cggcgacggc atcgcgatgg cctggcgcgc
      361 cggctgccgg gtcgcgaaca tggagttcat ccagttccac ccgacctgcc tgtaccaccc
      421 acaggaacgc agcttcctca tcaccgaggc gctgcgcggc gaaggcgcga tcctgcgcct
      481 gcccgacgcg ccgcccggat caccgccgag cgccggcacc cgcttcatgc cggcgcacga
      541 cgaacgtgcc gagctcgcgc cgcgcgacgt cgtcgcccgc gcgatcgact tcgagatgaa
      601 gaagcacggc ctggacaacg tgctgctcga cgcgacgcat ctcggcgaag acttcctgaa
      661 atcgcacttc ccgacgatcc acgcgcgctg cctgcagctc ggcatcgaca tcgcgcgcca
      721 ggccattccc gtcgtgccgg ccgcgcacta cacctgcggc ggcatcgtca ccgacctcga
      781 cggccgctgc gacctgcccg ggctgtacgc ggtcggcgag gccggctaca ccgggctgca
      841 cggcgcgaac cggctcgcga gcaactcgct gctcgaatgc gtggtgctcg gccgcacctg
      901 cgccgagcac gtgctgcacg cgccgcgggc gaacgcgccg gcgctgccgg cctgggacga
      961 gagccaggtc gaagacgccg acgagcaggt cgtgatctcg cacaactggg acgagctgcg
     1021 cctgctgatg tggaactacg tcggcatcgt gcgcacgacg cgccgcctcg agcgcgcgct
     1081 gcaccgcatc gagttgctgc gcagcgagat cgaggactac tacgcgaact tccgcgtcaa
     1141 ccgcgacctg ctcgagctgc gcaacctcgt cgactgcgcc gagctggtgg tgcgctcggc
     1201 gctgctgcgg cacgagagcc gcgggctgca ctacagccgc gacttcccgc agccgctgcc
     1261 ggtgagtttc gcgaccgtgc tgacgccccc ggcgcgccgc gacgccaacg ctcaggccac
     1321 gccggtcacg cgcatcgagt agtcgatcgc gcgcacgtcc ttcgtcagct tgccgaccga
     1381 gatgcggtcg acgccggtcg cggcgacgct gcgcagctga tcgagcgtga ggccgcccga
     1441 ggcttcgagc agcgcgcgcc cggcggtgat cgcgacggcc tcgcgcatcg cggcctcgcc
     1501 gaagttgtcg agcagcacgc tagccgcgcc ggccgcgagc gcctcgcgca gctgggtgag
     1561 cgtctcgacc tcgatctgga tctgcacgcc cgaattcagt gcggccgcat tcgccagcgc
     1621 cgccgccacg ccgccggccg cggcgatgtg gttctccttg atcaggatgc cgtgccacag
     1681 cgccagccgc tggttctgcc cgccgccgac ccgcaccgcg tacttctgcg cctggcgcag
     1741 cccgggcagc gtcttgcgcg tgtcgagcac cgcgcagccg cgcgggttcg gcgatgcgcc
     1801 cttgaccgcg ttcacgtgcg cgcgcgtgac ggtcgcggtg gccgacagca gctgcaggaa
     1861 gttcagcgcc ggccgctcgg ccgcgagcag cgcgcgcgcg tcgccctcga tctcgcagac
     1921 caggctgtcg gccggcatct cggcgccctc gtcgaactgc caggcgatcc acgccgcagg
     1981 gtcgagcgcg cgcacgcagc cgtcgaacca gtcgcgcccg cagagcacgg cgtgctcgcg
     2041 caccttgacg cgcgcccgca cgcggcggcc cgcggggatc agttggccgg tccagtcgcc
     2101 gcggccgatg tcttcggcca acgcatcgcg gatgttgcgt tcgcgcgcct gcgcaagcgt
     2161 ctcgtcggtg tcgaacatcg cggttcaggc cacgccgatg ttcggcacga agccttgctg
     2221 cgcgccgctg gcggcgaccg cggccgggtg cgcggcgacg aagtcgagca tgcgctcgat
     2281 gcagcccagt gcccggctgc gcaccggctc gggcacgctg atctcgccgc ggccgtgctc
     2341 gaggcacgcc agcacgccgg gcaggccgtt catcgccatc caggggcagt gcgcgcagct
     2401 cttgcaggtg gcgctctcgc ccgcggtcgg cgcttcgagc agaagcttgc ccggcgcgag
     2461 ctggcgcatg cggtgcatga tgccgttgtc ggtggcgacg atgtaggtcg gtgccttgcc
     2521 gtcggcgacc gccttgagca gctgtgtcgt cgagccgacc acgtcggcct gcgcgaccac
     2581 gctggccggc gactcggggt gcaccagcac ctgcgcgtcg gggtgttcgc gccgcagcag
     2641 ctcgagctcg atg
//
LOCUS       DASRDG010000568         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_88700, whole genome shotgun sequence.
ACCESSION   DASRDG010000568 DASRDG010000000
VERSION     DASRDG010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88700"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VFA35_09530"
     CDS             complement(<1..1164)
                     /locus_tag="VFA35_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT56455.1"
                     /translation="MTALVALIRAQRLATLAAVFAALSFVLPLGFSAKGQALHWPNVA
                     GPFVFACCGLFTALLAVLRPPRRLAPVLGFALLGALAALAPLILLATDVAAALDAATW
                     RSGFWCYTFALACLGVTTVRRLAALEPGGGFSAALSAYGTPLLFGAWVIVLWQLLVRA
                     FDVPQVLLPAPSAVWRALWASFGVLAGDFEQTVLHAVLIGLVAGSALGFVVGVLIDRS
                     AFLQRGLLPLAALASTVPLVGIAPIMVMWFGFDWHSKAAVVVVMTFFPMLVNTLAGLA
                     ESGHMERDLMRSYGAGYWRTLWSLRIPVALPFVFNALKVNSTLAMIGAIVAEFFGSPT
                     AGLGFRISTEAARLNMGLVWAAILVAALAGSLLYGLLGWIERRATFWHPSIRTG"
     gene            complement(1161..2060)
                     /locus_tag="VFA35_09535"
     CDS             complement(1161..2060)
                     /locus_tag="VFA35_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT56456.1"
                     /translation="MAATEARRGAAGALWFRAAPVLAVLGVVLVLWYALAVWLNAPQV
                     IERTLSAQPGWGAIDLLRGAWAMDRPVLPAPHQIALDLVGSIVDWPLDSPRNLLFHAA
                     VTSAATLLGFAMGTLLGAVLAVAIVHSRTLDRSLFPWIIASQTVPVLAIAPIIIVILG
                     SMGMTGTFPKAIISMYLCFFPVAVALVKGLRSPGVIETELLHTYAASKPQAFWKLRVP
                     ASLPFLFPALRVGIAAGLVGAIVGELPTGAQAGLGARLLTGSYYGNTVQIWSALVMSA
                     LLGLALTGLVALAERLLTPGGRR"
BASE COUNT          391 a          802 c          812 g          208 t
ORIGIN      
        1 gcccgtccgg atcgacgggt gccagaacgt cgcgcgccgc tcgatccagc cgagcagccc
       61 gtaaagcaag gagccggcca gcgcggcgac caggatcgcg gcccagacca ggcccatgtt
      121 gaggcgcgcc gcctcggtcg agatccggaa gccgaggccg gcggtcggcg agccgaagaa
      181 ctcggcgacg atcgcgccga tcatcgccag cgtcgagttg accttcagcg cgttgaacac
      241 gaagggcagc gcgaccggga tgcgcagcga ccacagcgtg cgccagtagc ccgcgccgta
      301 tgagcgcatc aggtcgcgct ccatgtggcc ggattcggcc agcccggcga gcgtgttcac
      361 cagcatcggg aagaaggtca tcacgacgac gaccgcggcc ttggagtgcc agtcgaagcc
      421 gaaccacatc accatgatcg gcgcgatgcc gaccagcggc acggtgctcg cgagcgccgc
      481 cagcggcagc agcccgcgct gcaggaacgc gctgcggtcg atcagcacgc cgacgacgaa
      541 gccgagcgcg gagccggcga cgaggccgat cagcaccgcg tgcagcacgg tctgctcgaa
      601 gtcgccggcg agcacgccga aggacgccca cagcgcgcgc cagaccgcgc tgggcgcggg
      661 cagcagcacc tgcggcacgt cgaacgcgcg caccagcagc tgccacagca cgatcaccca
      721 cgcgccgaac agcagcggcg tgccgtatgc cgacagcgcc gcgctgaagc cgccgcccgg
      781 ttcgagcgcg gcgaggcggc gcaccgtcgt gacgccgagg caggcgagcg cgaacgtgta
      841 gcaccagaag ccgctgcgcc aggtcgcggc gtcgagcgcg gcagccacgt cggtcgcgag
      901 caggatcagc ggcgcaagcg cggcgagcgc gcccagcagc gcgaagccca gcaccggcgc
      961 gagccggcgc ggcgggcgca gcaccgcgag cagcgccgtg aacaggccgc agcaggcgaa
     1021 cacgaaggga cccgccacgt tcggccagtg cagggcctgg cccttggcgc tgaaccccag
     1081 cggcagcacg aacgacagcg ccgcgaacac ggccgcgagc gtggccaggc gctgggcgcg
     1141 gatcagcgcg acgagcgcgg tcaccgccgg cctcccggcg tgagcagccg ttcggcgagc
     1201 gcgaccaggc cggtcagcgc caggccgagc agcgccgaca tcaccagcgc cgaccagatc
     1261 tgcaccgtgt tgccgtagta cgagccggtc agcagccgcg cgcccaggcc ggcctgcgcg
     1321 cccgtgggca gctcgcccac gatcgcgccg acgaggccgg ccgcgatgcc cacgcgcagg
     1381 gccgggaaca ggaacggcag cgaggctggc acgcgcagct tccagaacgc ctgcggcttc
     1441 gaggcggcgt aggtgtgcag caactcggtc tcgatcacgc ccggcgagcg caggcccttg
     1501 acgagcgcga ccgcgaccgg gaagaagcac aggtacatcg agatgatcgc cttcgggaac
     1561 gtgccggtca ttcccatgct gccgaggatc acgatgatga tcggcgcgat cgccagcacc
     1621 ggcacggtct gcgacgcgat gatccacggg aacaggctgc ggtcgagcgt gcgcgagtgc
     1681 acgatggcca ccgcgagcac cgcgccgagc agcgtgccca tcgcgaagcc gagcagggtc
     1741 gccgcgctcg tcacggcggc gtggaacagc aggttgcgcg gcgaatcgag cggccagtcg
     1801 acgatcgagc cgaccaggtc gagcgcgatc tggtgcggcg ccggcagcac cgggcggtcc
     1861 atggcccagg ccccgcgcag caggtcgatc gcgccccagc cgggttgcgc cgagagcgtg
     1921 cgttcgatga cctgcggcgc gttcagccag acggccagcg cgtaccagag cacgagcacg
     1981 acgccgagca ccgcgagcac cggcgcggcg cggaaccaca gcgcgcccgc ggcgccgcgg
     2041 cgcgcctcag tggccgccat gcccgtcctg cagcgccatg cgcacgcggt gcgcgatctc
     2101 ggcgaactcg ggcgtgtcgc ggatctcgag cgtgcggtcg gccggcaggc cggactcgat
     2161 cacgtcgacg atgcgcccgg ggcgcggcga catcacgacg atgcgcgacg aca
//
LOCUS       DASRDG010000569         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1348366, whole genome shotgun sequence.
ACCESSION   DASRDG010000569 DASRDG010000000
VERSION     DASRDG010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348366"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFA35_09540"
     CDS             <1..312
                     /locus_tag="VFA35_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZT56457.1"
                     /translation="LVLAVIVAGYVLQRIEEGVFIACFGIEMHIWQRFDSRFRLITAR
                     RNPNLLLLTVSVLLGRPDLGIVAVAVWTAASLLVHALRLLQAALARRGGKLQSWLAAP
                     P"
     gene            309..1304
                     /locus_tag="VFA35_09545"
     CDS             309..1304
                     /locus_tag="VFA35_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56458.1"
                     /translation="MSAPGRPESQYRRAAPEGCPARTELAALLDAELERAVPAPVTAL
                     AAELAARAGAATAAVLFYGSALRDVTLDGVLDFYVLTDKPIAWPGSRWTSLAGRVLPP
                     NVGYLETVVGAATLRTKYAVMTLAQFRDAMSAGGLDTTLWARFSQPVACIRARGDAAT
                     RAVREAIVEAVITAARWAAALGPERAEATAFWRSLYQRTYAAELRVERGARADDLVAR
                     DAARYAALLPAAWRAGGLDFAVGEDGLLAPALSAKERAAAERRWRRRRILGRPLNLLR
                     LLKAATTFDNAADYVAWKVERHSGYRIEPSEFQRRHPLLAAPALYLKLRARGVLR"
     gene            complement(1331..2266)
                     /locus_tag="VFA35_09550"
     CDS             complement(1331..2266)
                     /locus_tag="VFA35_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZT56459.1"
                     /translation="MTGATGFIGRHLVVALQRAGFRLRLLLRREPDVPEWRGLAAPEV
                     VAGALDDEAALERLVAGADVVIHLAGLIKAARRRDFFAVNRDGAAAMARAVERVAPGA
                     RFVLVSSLAAREPQLSDYAASKRAGEGAVLELLGARASVLRPSVVYGPGDRETLVFFQ
                     LAGGRLVPLLGSAEARAALIHVADLVRLIVAMARDPLPGAAVRSAADDRPQGYTWEEL
                     LGAAAQAVGNTAPRFVRAPRALLGGVALLGDLAKVFGSSSMISSQKLRELRHLDWGVA
                     AAERATAPGWAAQFDLERGFADAVAWYRKAGWLPA"
     gene            2244..>2951
                     /locus_tag="VFA35_09555"
     CDS             2244..>2951
                     /locus_tag="VFA35_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56460.1"
                     /translation="MKPVAPVTAASGGAALDSASCRERGIDVGRDAARGAGGSGPPRI
                     IGHMSLNPSERRFAVVTTCHAAGYETYGRTMIETFLAHWPAEVALLLYHEGFEPPTAP
                     GRIEAHDLMASCPELLDFKRRHADNPRANGQRPRWRRVRLGPLAMTLPLPRRRPSYRW
                     DAVRFAHKCYALFDAARRTDADVLIWIDADTRFFADVSLAELAVLAPVQSAVACLRRP
                     DFTETGFVAYNLRHPETP"
BASE COUNT          394 a         1141 c         1031 g          385 t
ORIGIN      
        1 ctggtgcttg ccgtcatcgt cgccggctat gtcctgcagc gcatcgagga aggcgtcttc
       61 atcgcctgct tcggcatcga gatgcacatc tggcagcgct tcgacagccg cttccgcctg
      121 atcacggcac ggcgcaatcc caacctgctg ctgctcaccg tgtcggtgct gctcggccgg
      181 ccggacctcg gcatcgtcgc ggtcgcggtc tggaccgccg cctcgctcct ggtccatgcg
      241 ctgcgcctgc tgcaggcggc gctggcgcgg cgcggcggca agctgcaatc gtggctcgcg
      301 gcacccccat gagcgcgccg ggccgtccag agagccaata ccggcgggca gcgcccgaag
      361 ggtgcccggc ccgcacggag ctcgccgcgc tgctcgacgc cgagctcgag cgggcggtcc
      421 ctgcgccggt gacggcgctg gccgccgagc tggcggcgcg cgccggcgcg gccacggccg
      481 cggtgctgtt ctacggctcg gcgctgcgcg atgtgacgct tgacggcgtg ctcgatttct
      541 acgtcctgac cgacaagccc atcgcctggc caggctcacg ctggacatcg ctggccggac
      601 gcgtgctgcc gcccaacgtc ggctacctcg agaccgtcgt cggcgcagcg acgctgcgca
      661 ccaagtacgc ggtcatgacg ctggcccagt tccgcgacgc gatgtcggcc ggcggcctgg
      721 acaccacgct ctgggcgcgc ttctcgcagc cggtggcctg cattcgcgcc cgcggcgatg
      781 ccgcgacgcg cgccgtacgc gaagcgatcg tggaggccgt catcactgcg gcacgctggg
      841 ccgcggcgct cggaccggag cgcgccgagg caaccgcctt ctggcgctcc ctctaccagc
      901 gtacctacgc cgccgagctg cgtgtcgagc gcggcgcccg cgccgacgac ctggtggcgc
      961 gcgacgccgc ccgctatgcg gcgcttctgc ccgcggcctg gcgtgccggc ggcctcgact
     1021 tcgccgtcgg cgaggacggc cttctggcgc cggcactgag cgcgaaggaa cgcgccgccg
     1081 ccgagcgccg ctggcggcgg cgtcgaatcc tcggccgacc gctcaacctg ctgcgcctgc
     1141 tgaaggcggc aacgaccttc gacaacgccg ccgactacgt cgcctggaag gtcgagcggc
     1201 acagcggcta caggatcgag ccgagcgaat tccagcgccg ccaccccttg ctcgcggcgc
     1261 cggcgctcta cctcaagctg cgcgcgcgcg gcgtgctgcg ctgatcgccg agcgccccga
     1321 ggcgctgccg ctaggccggc aaccagccgg cctttcgata ccaggccacg gcgtcggcga
     1381 agccgcgctc gagatcgaac tgcgcggccc agcccggcgc ggtcgcgcgt tccgccgcgg
     1441 cgacgcccca gtccagatgg cgcagctcgc gcagcttctg cgagctgatc atgctgctcg
     1501 agccgaagac cttcgccagg tcgccgagca gcgccacgcc gccgagcagc gcccgcggtg
     1561 cacgcacgaa gcgcggcgcc gtgttgccca cggcctgcgc cgcggcgccg agcagctctt
     1621 cccaggtgta gccctgcgga cggtcgtccg cggccgagcg caccgcggcg ccgggcagag
     1681 gatcgcgcgc catcgcgacg atcaggcgca ccaggtcggc gacatggatc aacgccgcgc
     1741 gcgcctcggc gctgccgagc aagggcacga gccggccgcc cgcaagctgg aagaacacca
     1801 gggtctcgcg gtcgcccggg ccgtagacga ccgaggggcg cagcacgctc gcgcgcgcgc
     1861 ccagcagctc gagcacggca ccctcgcccg cccgcttgct cgcggcgtag tccgagagct
     1921 gcggctcgcg cgccgccagg ctcgacacca gcacgaagcg cgcgcccggc gcgacgcgct
     1981 ccaccgcgcg cgccatcgcc gcggcgccgt cgcgattgac ggcgaagaag tcgcgccgcc
     2041 gcgctgcctt gatcagcccg gcgaggtgga tcacgacgtc ggcgcccgcc accaggcgct
     2101 cgagggccgc ttcgtcgtcg agcgcgccgg ccacgacctc gggcgccgcc agcccgcgcc
     2161 actcgggcac gtcgggctcg cggcgcagca gcaggcgcag gcgaaagcct gcccgttgca
     2221 aggcgacgac gagatgccgg ccgatgaagc cggtggcgcc ggtcacggcg gcgagcggcg
     2281 gagccgcgct cgattcggca tcctgccgcg aaagaggcat tgacgtaggc agggacgcgg
     2341 cccgtggcgc cggagggagc ggcccgccaa ggataatcgg acacatgtcg ctgaaccctt
     2401 ccgagcgccg cttcgcggtc gttaccacct gccacgccgc cggctacgag acttacggcc
     2461 ggacgatgat cgagaccttc ctcgcgcact ggccggccga ggttgcgttg ctgctgtatc
     2521 acgaaggctt cgagccgccg accgcgcccg ggcgcatcga ggcgcacgac ctcatggcat
     2581 cctgccccga gctgctcgac ttcaagcgac gtcatgccga caacccgcgg gccaacggcc
     2641 agcggccgcg ttggcgccgg gtgcgcctcg gcccgctggc catgacgctg ccgctgccgc
     2701 gccgccgtcc cagctaccgc tgggacgcgg tgcgcttcgc gcacaagtgc tacgccctct
     2761 tcgatgccgc gcggcggacc gacgccgacg tgctgatctg gatcgacgcc gacacccggt
     2821 tcttcgccga cgtcagcctt gccgagttgg cggtgctcgc gccggtgcag agcgccgttg
     2881 cctgcctgcg ccgaccggat ttcaccgaga ccggcttcgt cgcctacaac cttcgccatc
     2941 cggagacacc g
//
LOCUS       DASRDG010000570         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_891549, whole genome shotgun sequence.
ACCESSION   DASRDG010000570 DASRDG010000000
VERSION     DASRDG010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891549"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFA35_09560"
     CDS             <1..1011
                     /locus_tag="VFA35_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56461.1"
                     /translation="GSLMHELDDAVAGKYSVAVSQQRVNQKIANLGSVLLRAADRADR
                     DVSFDAIRAHMQALASMRELKARLPAEFFKVGNDLLVGLSGDATDLLGEDRIWVEHRI
                     ASQLVLAFKSVLGKMPDGVSAIAQAVKNAAHEEACRRNDAVFDLLVRVLNCFTREAVK
                     KKENASVFNVVYSYKALVRRLLVDRPECVPRLVQHLCFYAEYARVQGLPFIYELFSYE
                     LGELTARAYEGQAAPARELLDAALALGGVEQSVGLVKSRAILAAYFLERGLTQELELV
                     EASLHGAAPAVVESARRAILSVQDRVFWELNDRGLNFDFVEPRRRKRVAEVFDRVSAG
                     TG"
     gene            1067..2185
                     /locus_tag="VFA35_09565"
     CDS             1067..2185
                     /locus_tag="VFA35_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT56462.1"
                     /translation="MLVLSIVFFAFLAGVFWIGVALWFVAAFRLRGPDLSAFDQPSGE
                     RFSNGPQPSAELQAALASLARIGEAVRRVPRRQRTAALREAMDQLSAGRRFDASFTPV
                     DCGGVPGEWVLAPGADGTRRTLYIHGGAYAVGSPRSHRTLTSSFSAITGGAVLAIDYR
                     LMPEHPRIAGIEDCRRAYRWMLENGPAGAQPARTVFVAGDSAGGNLTLSLIAWLRDQG
                     LRAPDAAVALSPATDSTGASPSLKSNLHSDPMLGPLFAPLTKVPHPLLLWFGWLQNRI
                     NPRDPMISPVYGDLSRLPPVLVQVSQVEMLRDDGRRYVNRACAAGSPARLQTWDHMVH
                     VWQIFNPELPEARAALEEIRKFLAPHQARLTAAPASRA"
BASE COUNT          309 a          788 c          797 g          344 t
ORIGIN      
        1 ggcagcctga tgcacgagct cgacgatgcg gtcgcgggca agtattcggt ggcggtgtcg
       61 cagcagcgcg tcaaccagaa gatcgccaac ctcggcagcg tgctgctgcg cgccgccgat
      121 cgcgccgacc gcgacgtgag cttcgacgcg atccgcgcgc acatgcaggc gctggccagc
      181 atgcgcgagc tgaaggcgcg gctgccggcg gagttcttca aggtcggcaa cgacctgctc
      241 gtcggcctgt ccggcgatgc caccgacctg ctcggcgagg accgcatctg ggtcgagcac
      301 cggatcgcga gccagctcgt gctggccttc aagagcgtgc tgggcaagat gccggacggc
      361 gtcagcgcga tcgcccaggc ggtcaagaat gccgcgcacg aagaggcgtg ccggcgcaac
      421 gacgcggtgt tcgacctgct cgtgcgggtg ctgaactgct tcacgcgcga ggccgtcaag
      481 aagaaggaga acgcctccgt cttcaatgtc gtgtactcgt acaaggcgct ggtgcgccgc
      541 ctgctcgtcg accgtcccga gtgcgtgcca cggctggtgc agcacctgtg cttctacgcc
      601 gagtacgcgc gcgtgcaggg gctgcccttc atctatgaac tgttcagcta cgagttgggc
      661 gaactgacgg cgcgcgccta cgagggccag gcggcaccgg cgcgggagct gctcgatgcc
      721 gcgctcgccc tgggcggcgt cgagcagagc gtggggctgg tcaagtcgcg cgcgattctt
      781 gcggcctact tcctcgagcg cgggttgacg caggagctgg agctcgtgga ggcgtcgttg
      841 cacggtgcgg cgccggctgt cgtcgagagt gcgcggcgcg ccatcctgtc ggtccaggac
      901 cgggtgttct gggaactgaa cgaccgcggc ctcaacttcg acttcgtcga gccgcggcgc
      961 cgcaagcggg tggccgaggt cttcgatcgg gtgagcgccg gcacaggctg acctgtgtat
     1021 gctcgccggc ggcgcgacac ccgttccgtc gccgaaggga agcagcatgc tcgtcctgtc
     1081 gatcgtcttc ttcgcattcc tcgccggcgt gttctggatc ggcgtcgcgc tgtggttcgt
     1141 cgccgccttc cgtctgcgcg gccccgacct gtcggccttc gaccagccga gcggcgagcg
     1201 cttttcgaac ggcccgcaac ccagcgccga actgcaggct gcgctggcgt cgctggccag
     1261 aatcggcgaa gccgtgcgac gggtgccgcg acgccagcgc accgcggcgc tgcgcgaggc
     1321 gatggaccag ctgtccgccg gccgccgctt cgacgccagc ttcacgccgg tcgactgcgg
     1381 cggcgtgccc ggcgaatggg tgctcgcgcc gggcgccgac ggcacgcggc gcacgctgta
     1441 catccacggc ggcgcctacg cggtcggcag cccgcgcagc caccgcacgc tgacgagcag
     1501 tttctcggcg atcaccggcg gcgcggtgct cgcgatcgat taccgcctga tgcccgagca
     1561 cccgcgcatc gccggcatcg aggattgccg ccgcgcctac cgctggatgc tcgagaacgg
     1621 gccggccggt gcgcagccgg cgcgcaccgt gttcgtcgcg ggcgattcgg ccggcggcaa
     1681 tctcacgctg tcactgatcg cgtggctgcg cgaccagggc ctgcgcgcgc cggacgccgc
     1741 ggtggcgctg tcgccggcca ccgacagcac cggcgccagc ccgagcctga agtccaacct
     1801 gcacagcgat ccgatgctcg gcccgctgtt cgcgccgctg accaaggtgc cgcacccgct
     1861 gctgctgtgg ttcggctggc tgcagaaccg catcaacccg cgcgacccga tgatctcgcc
     1921 ggtgtacggc gacctgtcgc ggctgccgcc ggtgctcgtg caggtcagcc aggtcgagat
     1981 gctgcgcgac gacggccggc gctacgtcaa tcgcgcatgc gcggccggct cgccggcgcg
     2041 gctgcagacc tgggaccaca tggtccacgt ctggcagatc ttcaaccccg agttgcccga
     2101 ggcgcgcgcc gcgctggagg agattcgcaa gtttctcgcc ccgcaccagg cgcggctcac
     2161 agccgcgcca gcatctcgcg cgtgatcagc acgacgccgc tgtcggtgcg gtagaagcgc
     2221 cgcgcgtcct cggcggcg
//
LOCUS       DASRDG010000571         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_542473, whole genome shotgun sequence.
ACCESSION   DASRDG010000571 DASRDG010000000
VERSION     DASRDG010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542473"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFA35_09570"
     CDS             <1..864
                     /locus_tag="VFA35_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HZT56463.1"
                     /translation="CLTQLARRDAAALGGAMYAGPGAGIVVSGLFASGMVQWGWRAAA
                     GWAIFGVLAFVLAALVWRIFGTAATPQPAPGARAAAASNGAIDIAQGRAELGCLALAY
                     GIAGFGYIVTATFVPVIARAALPGSPWLDMFWPIFGLGVIAGALLSTRFEVSGDLRLL
                     LAGSYLVQAAGIFTSVWSPSLAGFAIGSLLLGLPFTAITFFAMQEVRRLRPALAASFM
                     GLLTATYGIGQIVGPPLVAWLLQRSASAGAGFALSLEIAASALVVGAGLYLGMVKAYP
                     MQRARVTLPRA"
     gene            complement(845..1198)
                     /locus_tag="VFA35_09575"
     CDS             complement(845..1198)
                     /locus_tag="VFA35_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HZT56464.1"
                     /translation="MKPSHHALVYSTDAGRMCPACRQPIKACACGRPVLPVGDGIVRV
                     SRETKGRAGKGVTLVKGLALDAPALAALGKQLKAACGSGGTVKDGVIEVQGDHVERVI
                     ELLKQDGRTVKRAGG"
     gene            complement(1257..2840)
                     /locus_tag="VFA35_09580"
     CDS             complement(1257..2840)
                     /locus_tag="VFA35_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZT56465.1"
                     /translation="MKFLTRLRAALGRMPIRPQLHASFAAVLLLTAAVGAVALTGLAR
                     VDQQAQALSDKWLRGVGLLSEIRVALIEARDHEVKHSRTGDTSYHSEYEEKLAAASTV
                     VGDALKTYAALASADDERALTAKFGKGWAGYQQAAQRVVALGRAKKQQDAADISDGVA
                     SMSFDETMSALNAMTKHDFAGGQAAAALTSAIYRQARWLVAALLGAAMLCGVALAYLI
                     TRHLLRQLGAEPSAASTLARSVAEGDLTTPIALRRGDTASLMAQLARMQTNLASAVTN
                     VRLGSENVATASAQIAQGNNDLSNRTEQQASALQQTAATMDELGATVRNNADNAKQAN
                     QLAQGASVVATRGGEVVGQVVETMKGINDSSKQIADIIGVIDGIAFQTNILALNAAVE
                     AARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIGASVGRVEQGTKLVDQAGQTMG
                     EIVQAIRRVSEIVAEISTASSEQSTGVVQVGQAISQMDQTTQQNAALVEQSAAAAESM
                     SQQARQLVDAVAVFKLHPA"
     gene            complement(2871..3362)
                     /locus_tag="VFA35_09585"
     CDS             complement(2871..3362)
                     /locus_tag="VFA35_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HZT56466.1"
                     /translation="MKLFPTAIAAALVLCAAGAASAADPKATIADLDARLAKIGAAKI
                     DGTDKVAGKTVPALYFGERKINNNYDVVDTVRRVHEASATIFVKDGDEFVRVSTNVLT
                     PEGKRGVGTQLARNKAYEALSKGASYCGPIDVLGTAFDACYDPIKDAGGKLVGVAYIG
                     HKK"
BASE COUNT          407 a         1332 c         1330 g          626 t
ORIGIN      
        1 tgcctcacgc agctggcgcg ccgcgacgcg gcggcgctcg gcggcgcgat gtacgccggg
       61 ccgggcgccg gcatcgtcgt cagcggcctg ttcgcgagcg gcatggtgca gtggggctgg
      121 cgcgcggccg ccggctgggc gatcttcggc gtgctcgcgt tcgtgctggc ggcgctggtg
      181 tggcgcatct tcggcaccgc cgccacgccg cagccggcgc cgggcgcgcg cgccgccgcg
      241 gcgtcgaacg gcgcgatcga catcgcgcaa ggccgcgccg agctcggctg cctggcgctc
      301 gcctacggca tcgccggctt cggctacatc gtcactgcca ccttcgtgcc ggtgatcgcg
      361 cgcgcggccc tgcccggctc gccctggctc gacatgttct ggccgatctt cggcctcggc
      421 gtgatcgccg gcgcgctgct gtcgacacgg ttcgaggtca gcggcgacct gcgcctgctg
      481 ctcgccggca gctacctggt ccaggccgcc ggcatcttca ccagcgtgtg gagtccgagc
      541 ctggccggct tcgcgatcgg cagcctgctg ctgggcctcc cgttcaccgc gatcaccttc
      601 ttcgcgatgc aggaggtgcg gcgcctgcgg ccggcgctcg cggcgagctt catggggctg
      661 ctgacggcga cctacggcat cgggcagatc gtcgggccgc cgctggtcgc gtggctgctg
      721 cagcgcagcg cgagcgccgg cgcggggttc gcgctctcgc tggagatcgc ggcgagtgcg
      781 ctggtggtcg gcgcggggct gtacctgggg atggtgaagg cgtacccgat gcagcgcgcc
      841 cgagtcaccc tcccgcgcgc ttgacggtcc ggccgtcctg cttcagcagt tcgatcaccc
      901 gctcgacgtg atcgccctgg acctcgatca cgccgtcctt caccgtgccg ccggagccgc
      961 acgccgcctt cagctgcttg ccgagcgcgg cgagcgcggg cgcatcgagc gcgagcccct
     1021 tgaccagcgt cacgcccttg ccggcgcggc ccttcgtctc gcgcgagacg cgcacgatgc
     1081 cgtcgccgac cggcagcacc ggccggccgc aggcgcaggc cttgatcggc tggcggcagg
     1141 ccgggcacat gcgcccggcg tcggtcgagt agacgagggc gtgatgcgaa ggcttcatgc
     1201 ggcgacgatc gcggcgcggt gccgccgcgt caatccgggc agatgcgagg ccggcttcag
     1261 gccggatgca gcttgaacac cgccaccgcg tcgacgagct ggcgtgcctg ctggctcatg
     1321 ctctcggcgg ccgcggcgct ctgctcgacc agcgcggcgt tctgctgcgt cgtctggtcc
     1381 atctgcgaaa tcgcctggcc gacctgcacg acgccggtgc tctgctcgct gctggcggtg
     1441 ctgatctcgg ccacgatctc gctgacgcgc cggatcgcct ggacgatctc gcccatcgtc
     1501 tgccccgcct ggtcgacgag cttcgtgccc tgctcgacgc ggccgacgct ggcgccgatc
     1561 agttccttga tctccttcgc cgcgccggcg ctgcgctgcg cgaggctgcg cacctcgctg
     1621 gcgacgaccg cgaagccgcg cccctgctcg ccggcgcgcg ccgcctcgac cgcggcgttc
     1681 agcgccagga tgttggtctg gaacgcgatg ccgtcgatca cgccgatgat gtcggcgatc
     1741 tgcttgctgc tgtcgttgat gcccttcatc gtctcgacga cctggccgac gacctcgccg
     1801 cccctggtcg cgaccaccga cgcgccctgc gcgagctggt tcgcctgctt cgcgttgtcg
     1861 gcgttgttgc gcacggtcgc gccgagctcg tccatcgtcg ccgcggtctg ctgcagcgcg
     1921 ctggcctgct gctcggtgcg gttcgagagg tcgttgttgc cctgcgcgat ctgcgcgctg
     1981 gcggtggcga cgttctcgga gccgagccgc acgttggtga cggccgaggc gaggttggtc
     2041 tgcatgcgcg cgagctgcgc catcaggctc gcggtgtcgc cgcgccgcag tgcgatcggc
     2101 gtcgtcagat cgccctcggc gaccgagcgc gccagcgtcg atgcggcgct cggctcggcg
     2161 ccgagctggc gcagcaggtg gcgcgtgatc aggtacgcga gcgcgacgcc gcacagcatc
     2221 gctgcgccga gcagcgcggc gaccagccag cgcgcctggc ggtagatcgc cgaggtcagc
     2281 gccgcggcgg cctggccgcc cgcgaagtcg tgcttggtca tcgcgttgag cgcgctcatc
     2341 gtctcgtcga aggacatgct ggcgacgccg tcgctgatgt cggcggcgtc ctgttgcttc
     2401 ttcgcgcggc cgagcgcgac gacgcgctgc gccgcctgct ggtagcccgc ccagcccttg
     2461 ccgaacttcg cggtcagcgc gcgctcgtcg tcggcactcg ctagcgccgc gtaggttttc
     2521 agcgcgtcgc ccaccaccgt gctcgcggcc gcgagcttct cctcgtactc cgagtggtag
     2581 ctggtgtcgc cggtgcggct gtgcttgacc tcgtggtcgc gcgcctcgat cagcgcgacg
     2641 cggatctccg agagcaggcc gacgccgcgc agccacttgt cggacagcgc ctgcgcctgc
     2701 tggtcgacgc gtgccaggcc ggtcagcgcg actgcgccga ccgcggcggt cagcagcagc
     2761 acggcggcga acgacgcatg gagttgcggg cggatcggca tgcggccgag tgccgcccga
     2821 aggcgggtga ggaacttcat cgcgtctctc cgagggtgcg gcgggtgggc ctacttcttg
     2881 tggccgatgt aggcgacgcc gacgagcttg ccgccagcgt ccttgatcgg gtcgtagcac
     2941 gcgtcgaacg cggtgccgag cacgtcgatc gggccgcagt acgacgcgcc cttgctgagc
     3001 gcctcgtagg ccttgttgcg cgcgagctgc gtgcccacgc cgcgtttgcc ctcgggcgtg
     3061 agcacgttgg tgctgacgcg cacgaactcg tcgccgtcct tgacgaagat cgtcgcgctg
     3121 gcttcgtgga ccctgcgcac cgtgtcgacg acgtcgtagt tgttgttgat cttgcgctcg
     3181 ccgaagtaca gcgccggcac ggtcttgccg gcgaccttgt cggtgccgtc gatcttggcg
     3241 gcgccgatct tcgccagacg cgcgtcgagg tcggcgatgg tggccttcgg gtcggcggcg
     3301 ctggccgcgc cggcggcaca caagacgagg gccgcggcga tggcggtcgg aaagagcttc
     3361 atggtgagtc cgatcggttg gcaggggttg gtgatgctgc ggcgtgggcg gccatgggcc
     3421 gcgaacctgc tttatcgacg ccaagctcgc ggacttgaac cgcgcacccc gcatggaccg
     3481 cacgcggcat ggggcgttat gtagtgcgct atatttcgat cccgctcatc acgtcgaccc
     3541 gccatgcacc cgctcagact ccgccgccgc ctgctcgccg cactgcttgc cgcaccgctc
     3601 gccgtttccg gcctcgcggc gcaggccgcc gacctgaccg tctccgccgc ggcgagcctg
     3661 accaacgcgc tgcgcgaact cggcccggcg ttcga
//
LOCUS       DASRDG010000572         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1121160, whole genome shotgun sequence.
ACCESSION   DASRDG010000572 DASRDG010000000
VERSION     DASRDG010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121160"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..531)
                     /gene="lspA"
                     /locus_tag="VFA35_09590"
     CDS             complement(43..531)
                     /gene="lspA"
                     /locus_tag="VFA35_09590"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZT56467.1"
                     /translation="MATTTRSPLLPWLGIAAIVVLLDQITKTMILKTMAIGDSHTVNA
                     FFNLVHWRNTGAAFSFLHASSGWQRWLFVGLAVTAAVFIVWMLSRHGGQRLFGWALAL
                     ILGGAVGNVIDRLLHGSVVDFIQLHWNGAAFPSFNVADSAITIGAGLLILDELLRVRR
                     GR"
     gene            597..809
                     /locus_tag="VFA35_09595"
     CDS             597..809
                     /locus_tag="VFA35_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56468.1"
                     /translation="MNDDTLIVVLVEDFKRKTSHERSVKRGGSDPKALRGSLQARRCG
                     LNRNPEFAAQASLLRFVPPVCGLDVR"
     gene            complement(839..1078)
                     /locus_tag="VFA35_09600"
     CDS             complement(839..1078)
                     /locus_tag="VFA35_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HZT56469.1"
                     /translation="MHKDHAMAPQPEFADLFELPPAERLQLVEDLWDSLIPREAELPV
                     SDWVVRELHERKKRFDANPESGLTWEEVVRGLDPD"
     gene            complement(1078..>2314)
                     /locus_tag="VFA35_09605"
     CDS             complement(1078..>2314)
                     /locus_tag="VFA35_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZT56470.1"
                     /translation="ILDVWFDSGSTFFHVLRGSHPGTTVPDDDGAAPEADLYLEGHDQ
                     HRGWFHSSLLIACALEGHAPYRGLLTHGFTVDGSGRKMSKSLGNFVSLQESVQKRGAE
                     IIRLWCAATDYSGDLAIDEKILDRVVDSYRRIRNTLRFLLANTSDFDPAKDAVPLGEL
                     LEIDRWALARAAQVQAEFVGDFDTARGVFEGGHYKVYEFHPVVAKLQLFCSEDLGAFY
                     LDVLKDRLYTTAPKSLARRSAQTALWHITHAMLRWMAPFLSFTAEEAWQLFGTSPSIF
                     TETYADTSAWGGAELLAKWARIRAIRDAANKEIEAVRTAGLLGSSLQANLTIAANDAD
                     HALLASLGDDLRFVTITSQALLSAAPELHVEATPSSATKCERCWHYRDDVGADAAHPT
                     ICGRCVSNLYGAGERRTVA"
BASE COUNT          383 a          745 c          813 g          373 t
ORIGIN      
        1 cgcaagacga ccgggagcag catactgatc ccgtaggtcg gatcagcggc cccgacgaac
       61 gcgcagcaac tcgtcgagga tcagtagccc ggcgccgatc gtgatcgcgc tgtcggcgac
      121 gttgaacgac gggaaggccg ctccgttcca gtgcagctgg atgaagtcga ccaccgagcc
      181 gtgcagcagc cggtcgatca cgttgccgac cgcgccgccg aggatcagcg ccagcgccca
      241 gccgaacagg cgctggccgc cgtggcggct gagcatccag acgatgaaga ccgccgcggt
      301 gaccgcgagg ccgacgaaca gccagcgctg ccagcccgac gacgcgtgca ggaacgagaa
      361 cgcggcgccg gtgttgcgcc agtgcacgag gttgaagaac gcgttgacgg tgtggctgtc
      421 gccgatcgcc atcgtcttca ggatcatcgt cttcgtgatc tggtcgagca gcaccacgat
      481 cgcggcgatg ccgagccagg gcagcagcgg gctgcgggtt gtcgttgcca tgtgggtcag
      541 ggggtaccga gtcgctcgcg ccacttcgtc gggtcttgcg aggtgtggaa cacgagatga
      601 acgacgacac gctcatcgtc gtactcgtag aagatttcaa aaggaaaacg tcgcacgagc
      661 gctcggtgaa acggggcgga agcgatccga aagcgctcag gggatcgttg caagcgcgac
      721 gttgcggcct caaccgcaat ccggaattcg cggcccaggc ctcgttgctg cgattcgtac
      781 caccggtgtg cggccttgac gtccgctgag gcctccggcg tgatgatgag gcgccgcatc
      841 aatccggatc cagcccccgg acgacttctt cccaggtcag cccgctttcg gggttcgcgt
      901 cgaagcgctt cttgcgctcg tgcaactcgc gcacgaccca gtcggagacc gggagttcgg
      961 cttcgcgtgg gatgaggctg tcccacaggt cttcgaccag ttgcagccgc tctgccggcg
     1021 gcaactcgaa caggtcagcg aactcgggct gcggtgccat tgcgtggtcc ttgtgcatca
     1081 cgccactgta cggcgttcgc cggcgccgta caaattgctg acgcagcgcc cgcagatcgt
     1141 cgggtgcgcc gcgtcggcgc ccacgtcgtc gcggtagtgc cagcagcgct cgcacttggt
     1201 cgcgctcgac ggcgtggcct cgacgtgcag ctccggtgcg gccgagagca acgcctgcga
     1261 ggtgatcgtg acgaagcgca ggtcgtcgcc cagcgacgcg agcagcgcgt ggtcggcgtc
     1321 gttcgcggcg atcgtcaggt tcgcctgcag cgacgagccg agcagtccgg ccgtgcgcac
     1381 cgcctcgatc tccttgttcg cggcgtcgcg gatcgcgcgg atgcgcgccc acttcgcgag
     1441 cagttcggcg ccgccccagg cgctggtgtc ggcgtaggtc tcggtgaaga tcgacggcga
     1501 ggttccgaac agctgccacg cttcctcggc cgtgaagctc aggaacggcg ccatccagcg
     1561 cagcatcgcg tgcgtgatgt gccacagcgc cgtttgcgcc gagcggcgcg ccaggctctt
     1621 tggcgcggtc gtgtacaggc ggtccttcag cacgtcgagg tagaaggcac cgaggtcctc
     1681 ggagcagaac agctgcagct tggccaccac cgggtggaac tcgtagacct tgtagtggcc
     1741 accttcgaac acgccgcgcg cggtatcgaa gtcgccgacg aactcggctt gcacctgcgc
     1801 cgcgcgcgcc agcgcccagc ggtcgatctc gagcagttcg ccgagcggca cggcgtcttt
     1861 ggcgggatcg aagtcgctgg tgttcgcgag cagaaagcgc agcgtgttgc ggatgcgccg
     1921 gtacgaatcg acgacgcggt cgaggatctt ctcgtcgatc gcgaggtcgc ccgagtagtc
     1981 ggtcgcggcg caccacagcc ggatgatctc ggcgccgcgc ttttgcaccg attcctgcag
     2041 cgagacgaag ttgcccaggc tcttgctcat cttgcggccc gagccgtcga cggtgaagcc
     2101 gtgggtcaag aggccccggt agggcgcgtg gccctcgagc gcgcaggcga tcagcagcga
     2161 cgaatggaac cagccgcggt gctggtcgtg gccctcgaga tagaggtcgg cctcgggcgc
     2221 ggcgccgtcg tcgtcgggca cggtcgtgcc cggatggctg ccgcgcagga cgtggaagaa
     2281 ggtcgagccc gagtcgaacc agacgtcgag gatg
//
LOCUS       DASRDG010000573         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1694216, whole genome shotgun sequence.
ACCESSION   DASRDG010000573 DASRDG010000000
VERSION     DASRDG010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694216"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFA35_09610"
     CDS             complement(<1..757)
                     /locus_tag="VFA35_09610"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZT56471.1"
                     /translation="MSFLAIDVGNTRLKWAQYASPQPGASLLNHGAVFLETIDDLAEK
                     EWRTLPAPSSMLGCVVAGEGVRRRVAEQLEIWDVEPRWVVSGASACGVTNGYDHPSRL
                     GVDRWVALIGARHRVLAQGAARPALVVMVGTAVTVDALDASGRFLGGLILPGFGLMLR
                     ALEMGTAGLKAPTGEVVEFPTNTSDALMSGGADAIAGAVERMHRKLLARTGQAPLLMM
                     TGGAAVKLAPITDLPFETVDTLIFEGLLQLQSHR"
     gene            complement(754..1455)
                     /locus_tag="VFA35_09615"
     CDS             complement(754..1455)
                     /locus_tag="VFA35_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56472.1"
                     /translation="MAAGLVPIKTPEGQAELATRQRRVSQRHRTVLFLVDGKRSAPEV
                     RALALQAGVPESCFDELVAMGLIMLPEPTFSMLTDTAALPDVLHVDLPLSGFNAGGVG
                     TTVDSVLPPSRSLYPTLSTDSSLGDSPVPSGWLPSEAGAPGGTLDPAVAEARLILLRA
                     VRTEAPLAGSLTLLRLRRARTRDELVGLLDEVEARITKPHRALAAGQTMRRVRHLLGL
                     RVDPSLASADRQSTP"
     gene            1689..>2446
                     /locus_tag="VFA35_09620"
     CDS             1689..>2446
                     /locus_tag="VFA35_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HZT56473.1"
                     /translation="MRKGAKKFDLKRFDPAARPFGSGDKARDKEAAAALAVELDALQN
                     LFYADKRFKLLVVLQGTDTSGKDGTIRGVFGQMSALGVRTVGWKAPTEPERTHDYLWR
                     IHSQVPGSGEVTIFNRSHYEDVLVPPVNGWITPEQTAQRYAQINDFERLLTETGTVIV
                     KFLLLISKNEQRVRLQERLDDPTKNWKFAVGDLDARKQWDAYQKAYSAAVAATGTPWA
                     PWTIVPADSKTHRNLMIATVMKQVLTGLKLRFPPP"
BASE COUNT          408 a          854 c          854 g          330 t
ORIGIN      
        1 atcgatgcga ctgaagctgc agtaggcctt cgaaaatcag cgtgtcgacc gtctcgaacg
       61 gcaggtcggt gatcggcgcg agtttcaccg cagcgccgcc ggtcatcatc agcagcggcg
      121 cttgaccggt tcgcgcgagc agcttgcggt gcatgcgctc gaccgcgccg gcgatcgcgt
      181 cggcgccgcc gctcatcagt gcgtcgctgg tgttggtcgg gaattcgacg acctcgccgg
      241 tgggcgcctt caacccggcc gtgcccatct cgagggcgcg cagcatcagg ccgaagccgg
      301 gcaggatcag cccgcccaga aagcgcccgc tcgcatccag cgcatcgacg gtgacggccg
      361 tgcccaccat caccaccagc gcgggccgcg ccgccccttg cgcgagcacg cggtggcgcg
      421 cgccgatcag cgcgacccag cggtcgacgc cgagccggct cgggtggtca tagccgttgg
      481 tcacgccgca ggccgaggcg cccgagacga cccagcgcgg ctcgacgtcc cagatttcga
      541 gttgctccgc gacgcgccgc ctcacgcctt cgccggcgac cacgcagccg agcatgctgc
      601 tcggcgcggg cagcgtgcgc cactccttct cggccaggtc gtcgatggtt tcgagaaaga
      661 ccgcgccatg gttcagcagg ctcgcgccgg gttggggcga ggcgtactgc gcccacttca
      721 ggcgcgtgtt gcccacgtcg atcgcgagaa agctcatggc gtggattgtc ggtcggccga
      781 tgccagcgac ggatcgaccc gcaagcccag caggtgccgc acgcggcgca tcgtctggcc
      841 ggcggccagc gcgcggtgcg gcttggtgat gcgcgcctcg acctcgtcga gcaggccgac
      901 gagttcatcg cgggtgcgcg cgcggcgcag gcgcagcagg gtcagcgagc cggccagcgg
      961 cgcttcggtg cgcaccgcgc gcagcaggat cagccgcgcc tcggcgacgg ccgggtcgag
     1021 cgtgccgccg ggcgcgcccg cttcggacgg cagccagccg ctgggcacgg gcgaatcgcc
     1081 gagcgacgag tcggtcgaca gcgtggggta gaggcttcgc gacggcggca ggaccgagtc
     1141 gacggtggta ccgacaccac cggcattgaa ccccgacagc ggcaggtcga cgtgcagcac
     1201 atccggcagc gccgcggtgt cggtcagcat cgagaaggtc ggctcgggca gcatgatcag
     1261 gcccatcgcg accagctcgt cgaagcagct ctcgggcacg ccggcctgca gcgccagggc
     1321 gcgcacctcg ggtgcgctgc gtttgccatc gaccaggaac agcacggtgc ggtggcgctg
     1381 gctgacgcgg cgctggcgcg tggcgagctc ggcctgccct tcgggggtct tgatcggaac
     1441 caatcctgcg gccaagcgaa tctcctgtca tcactgcccg tccggcgaag gctccgtggc
     1501 cccgcgcaag cccggatacg cagcatcccg cccggcgcga ttgtgccgcc aatccccgcc
     1561 ggcggcgata ggaggcgata cactcgcgat gtgacgtata cgtcaattga ccgacacccg
     1621 gaggccgcgt ggcgaagaag acaccccagt gggcggagca gttggcgcac taccgattcg
     1681 acgccgcgct gcgcaaaggc gccaagaagt tcgacctgaa acgcttcgac cccgccgccc
     1741 ggccgttcgg cagcggcgac aaggcgcgcg acaaggaagc cgcggccgcg ctcgcggtcg
     1801 agctcgacgc gctgcagaac ctgttctacg ccgacaagcg cttcaagctg ctggtcgtgc
     1861 tgcagggcac cgacacctcg ggcaaggacg gcacgatccg cggcgtgttc ggccagatga
     1921 gcgcgctcgg cgtgcgcacc gtcggctgga aggcgccgac cgaacctgaa cgcacgcacg
     1981 actacctctg gcgcatccac agccaagtgc ccggcagcgg tgaggtgacg atcttcaacc
     2041 gcagccacta cgaagacgtg ctggtgccgc cggtcaatgg ctggatcacg cccgagcaga
     2101 cggcgcagcg ctacgcgcag atcaacgact tcgagcgcct gctgaccgag accggcacgg
     2161 tgatcgtcaa gttcctgctg ctgatctcga agaacgagca gcgcgtgcgc ctgcaggagc
     2221 ggctcgacga cccgaccaag aactggaagt tcgcggtcgg cgacctcgat gcccgaaagc
     2281 agtgggacgc gtatcagaag gcctacagcg ccgcggtggc cgcgaccgga acaccgtggg
     2341 cgccgtggac catcgtgccg gccgactcga agacccaccg caacctgatg atcgcgaccg
     2401 tgatgaagca ggtgctgacc gggttgaagc tgcgtttccc gccacc
//
LOCUS       DASRDG010000574         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_892261, whole genome shotgun sequence.
ACCESSION   DASRDG010000574 DASRDG010000000
VERSION     DASRDG010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892261"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFA35_09625"
     CDS             complement(<1..193)
                     /locus_tag="VFA35_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT56474.1"
                     /translation="MIRRIVAFALYQPLFIVLGMALFIGGGVWAFINLPVEAFPDVTD
                     TQVTVVSLYPGRAAEEVELQ"
     gene            complement(200..1318)
                     /locus_tag="VFA35_09630"
     CDS             complement(200..1318)
                     /locus_tag="VFA35_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZT56475.1"
                     /translation="MKRALAGLCVVLLLAACGDKNGKEGAAQPTVKDHSVVIFPAGSP
                     QVQQLISVPIEPRRETTLLFNGRLVWNEDRTVRVFPPFAGRVVSIAVRPGDRVKPGQT
                     LAVLAAPELGVAQADVRRAEQDYALAQKNLKRIRELHAAGVAPQKDLQTAQADVARMA
                     AERQRAAAKLKPYGKTNIVDQQLALTSPVEGVVVERNLNPGQELRPDAQGDKALFVVS
                     DPSQLWFVLDVTEKDIGSVKPGTEVRIASTSLGDDRINGRIDHVADVVDPQTRTVKAR
                     GSIQSTDERLKAEMYIVAKVHVPATSGFLVPARAVYLRGEQYFVFVDDGNGHYTRRAV
                     LVGSIADGKQVILEGLAADQRVVVDGNLQLERLLASRD"
     gene            complement(1315..>2248)
                     /locus_tag="VFA35_09635"
     CDS             complement(1315..>2248)
                     /locus_tag="VFA35_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZT56476.1"
                     /translation="AASGLQRAAQADSLDLVRQQLAALRGAYFDLKQAQEKAEVLAGT
                     ADLFSNTLAAAQRRLKAGDLSQADVAKVQVDYERAQNDARAARGEFARNQIALAYMLG
                     LEADAAELRPVDAWPAPERAYPDTIEQAIDARPDVLAAKARLEAAEKLRDLARAQRTR
                     DVTIGAQYEHYPGSVPVNSVGFGVAIPLFTGNSFEGDIQKAEVDRYASIDALARARAV
                     AGNDLRRAAGDLNSAADRLERFDGSLLGAAARSAQAAEFAFQRGAISVLEVLDARRTH
                     RAVQLEALASRTDHAKALAAWRASLTSAESLKGK"
BASE COUNT          318 a          797 c          775 g          358 t
ORIGIN      
        1 cctgcagctc cacctcttcc gccgcgcgcc ccgggtagag cgacaccacc gtcacctgcg
       61 tgtcggtgac gtcggggaag gcttccaccg gcaggttgat gaaggcccac acgccgccgc
      121 cgatgaacag cgccatgccg agcacgatga acagcggctg gtacagcgcg aacgcgacga
      181 tgcgccggat cacgggcgcc tagtcccgcg aggcgagcag gcgctcgagc tgcaggttgc
      241 cgtcgacgac cacgcgctgg tcggcggcga gtccctcgag gatcacctgc ttgccgtcgg
      301 cgatcgaccc caccagcact gcgcggcgcg tgtagtgtcc gttgccgtcg tcgacgaaga
      361 cgaagtactg ctcgccgcgc aggtacaccg cgcgcgccgg gacgagaaat cccgacgtcg
      421 ccgggacgtg caccttggcg acgatgtaca tctcggcttt caggcgctcg tcggtggact
      481 ggatgctgcc gcgcgccttg acggtgcgcg tctgcggatc caccacgtcc gcgacgtgat
      541 cgatgcgtcc gttgatgcga tcgtcgccga gggaagtgct ggcgatcctc acttcagtgc
      601 ccggcttgac gctgccgata tccttttcgg tcacgtcgag cacgaaccag agctgcgacg
      661 gatccgaaac cacgaacaat gccttgtcgc cctgcgcatc gggacgcagc tcctggccag
      721 ggttgaggtt gcgctccacg acgacgcctt ccaccgggct tgtcagcgcg agctgctggt
      781 cgacgatatt ggttttgccg tagggcttga gcttggccgc cgcgcgctgc cgctcggccg
      841 ccatgcgcgc cacatcggcc tgcgccgtct gcaggtcttt ctgcggtgcc acgcccgcgg
      901 catgcagctc ccggatgcgc ttcaggttct tctgcgccag cgcgtagtcc tgctcggccc
      961 ggcgcacatc ggcctgcgcg acgccgagct ccggcgcagc cagcaccgcc agcgtctgcc
     1021 ccggcttcac ccgatctccg ggacgaaccg cgatggacac gacccggccg gcgaacggcg
     1081 ggaagacgcg aaccgtgcgg tcctcgttcc acaccaggcg gccgttgaag agcagggtcg
     1141 tttcgcgccg cggctcgatc ggcaccgaga tgagctgctg aacttgcggg ctgcccgcgg
     1201 ggaagatcac cacgctgtgg tccttcaccg tcggctgggc agcgccttcc ttgccgttct
     1261 tgtcgccaca ggcggcgagg agcagcacga cgcagaggcc ggcgagggcc cgcttcattt
     1321 gcccttcagg ctttcggcgc tggtcaggct cgcgcgccag gcggcgagcg ccttcgcatg
     1381 atcggtgcgg gacgcgagcg cctcgagctg taccgcacga tgcgtgcgcc gcgcgtcgag
     1441 cacctcgagg accgagatcg cgccgcgctg gaaggcgaac tcggcggcct gcgcgctacg
     1501 cgctgcggca ccaagcagcg agccatcgaa gcgctccagc cggtcggcgg cggagttgag
     1561 gtcacccgcc gcgcgccgca aatcgttgcc ggcgacggcg cgcgcccgcg cgagcgcgtc
     1621 gatcgatgcg tagcggtcga cctcggcctt ctggatgtcg ccctcgaagc tattgccggt
     1681 gaagagcggg atggcgacgc cgaagcccac cgagttcacc ggcacgctgc cgggatagtg
     1741 ctcgtattgc gcgccaatgg tcacgtcacg cgtgcgctgt gcgcgcgcca ggtcgcgcag
     1801 cttctcggct gcctcgagcc gcgccttcgc ggcgagcacg tcgggccggg cgtcgatcgc
     1861 ctgctcgatc gtgtccggat aggcgcgctc gggcgcgggc caggcgtcca ccggccggag
     1921 ctctgcagcg tcggcctcca gcccgagcat ataggcgagc gcgatctggt tcctggcgaa
     1981 ctcgccgcgc gccgcgcgcg cgtcgttctg cgcgcgctcg tagtcgacct gcaccttggc
     2041 gacgtctgcc tgggagagat cgccggcctt cagccgccgc tgcgccgcgg cgagcgtgtt
     2101 cgagaagagg tcggcggtgc cggcgagcac ctcggctttt tcctgcgcct gcttgagatc
     2161 gaagtacgcg ccgcgcagcg ccgccagctg ctggcgcacc aggtccagcg aatccgcctg
     2221 cgccgcacgc tgcaagccgg aggctgcg
//
LOCUS       DASRDG010000575         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1694472, whole genome shotgun sequence.
ACCESSION   DASRDG010000575 DASRDG010000000
VERSION     DASRDG010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694472"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1508)
                     /locus_tag="VFA35_09640"
     CDS             complement(159..1508)
                     /locus_tag="VFA35_09640"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HZT56477.1"
                     /translation="MDQPAAAAAVAVPPPAPVAAAERIASIDVIRGLAMLGILVMNVV
                     EFAWPFDAYDNPAYAGGSTGLDLATWFVQATLFDGKMRALFSMLFGAGLVLIDQRMQA
                     AGRGGEAADLLLRRCLWLVPFGIVHRFVLQWSGDILYQYGLLALLAIPFRRWPARRLL
                     ALGIAVLALAAVPEAWGYRGLDRLRSDAAAAAALAAKGEPLPAPLATAQKDWKRYANR
                     VPPEPETLAAQVQAMRKGYVDICTERWDYHHKFQSIYLYEYFVYDVLGMMLLGMGLMK
                     TGFFAGACSPRVYRRVLVAGGIAALAVLAWSIAWQQSGFSNAALGLRLVRGAGYSCTR
                     ALVGLAWAAAVLLLLRAGRLRLLTAALASVGRMAFSCYIVQTLCCTLWFFGYGFGHYG
                     DMTRSALMVVVAVVSLIQIAFSTAWLRLFRFGPLEWAWRSLTYWRRQPLLRARAPSG"
     gene            complement(1663..>2022)
                     /locus_tag="VFA35_09645"
     CDS             complement(1663..>2022)
                     /locus_tag="VFA35_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56478.1"
                     /translation="NVLTATTLPWTGSTYRWLATGMPGNAIALDVLGLSTMSVPLSSI
                     LPQGVAGCALLASPDVFDFLLPAGGSVRAQFAIPNAVALAGQVVHQQVGSVEFGVLGN
                     VTALTSTNGLSLTIGTF"
BASE COUNT          349 a          723 c          725 g          225 t
ORIGIN      
        1 gagaagcaac ctgggacgca cgcctatgcc gacaatgcga agaagtaccg caccgcggcc
       61 gaggaggcgt tccagaggct gcggcgttcc ttccccgacg cgacgatgcc cgacgggcag
      121 cgcgtcgtcg acgtgacgcg cgccgaggtg tccggccgct agccggacgg cgcgcgcgcg
      181 cgcagcagcg gctgccgccg ccagtaggtc agcgagcgcc acgcccactc gagcggcccg
      241 aagcggaaca ggcgcagcca cgctgtgctg aacgcgatct ggatcagcga cacgaccgcc
      301 acgacgacca tcaacgcgct gcgcgtcatg tcgccgtagt ggccgaagcc gtagccgaag
      361 aaccacagcg tgcagcagag cgtctgcacg atgtagcagc tgaacgccat gcggccgacg
      421 gacgcgagcg ccgccgtcag aaggcgcaac cggcccgcgc gcagcagcag cagcaccgcc
      481 gcggcccacg cgaggccgac cagcgcgcgc gtgcacgagt agccggcgcc gcgcacgagc
      541 cggagcccga gcgccgcgtt cgagaacccg ctctgctgcc acgcgatcga ccaggcgagc
      601 acggcgagcg cggcgatccc gccggcgacc agcacgcggc ggtagaccct cgggctgcac
      661 gcgccggcga agaagccggt cttcatcagc cccatgccga gcagcatcat gccgagcacg
      721 tcgtagacga agtactcgta gaggtagatc gactggaact tgtggtggta gtcccaccgt
      781 tcggtgcaga tgtcgacgta gcccttgcgc atcgcctgca cctgcgcggc cagagtctcg
      841 ggctcgggcg ggaccctgtt cgcgtacctc ttccagtcct tctgcgcagt cgccagcggc
      901 gcgggcaacg gctcgccctt cgcagccagc gccgcggcag ccgcggcatc gctgcgcagc
      961 ctgtcgagcc cgcgatagcc ccacgcctcg ggcaccgcag ccagcgccag cacggcgatg
     1021 ccgagcgcga gcaggcggcg cgccggccag cgccggaacg ggatcgcgag cagcgcgagc
     1081 aggccgtact ggtagaggat gtcgccgctc cactgcagca cgaagcggtg cacgatgccg
     1141 aacggcacca gccacaggca gcgccgcagc agcaggtcgg cggcctcgcc gccgcggccg
     1201 gccgcctgca tgcgctggtc gatcagcacg agcccggcgc cgaacagcat cgagaacagc
     1261 gcgcgcatct tgccgtcgaa cagcgtcgcc tggacgaacc aggtcgcgag gtcgaggccg
     1321 gtgctgccgc ccgcgtaggc ggggttgtcg taggcgtcga agggccaggc gaactcgacg
     1381 acgttcatca ccaggatgcc gagcatcgcc aggccgcgga tcacgtcgat gctggcgatg
     1441 cgttcggcgg cggcgacggg cgcgggcggc gggacggcga ccgcggcggc tgcagcaggc
     1501 tggtccatgc gatgcctacg gtccggcggc cgcgcgcgcg ggctcacgac gcgcaattcc
     1561 cggcacgcgg gcagacgagg gagccgacgc tcacaggcgc cggacgccac gttgctggca
     1621 gcgaccgcga ggtctcgctg gccgtgcagc gaccctggag gctcagaaag tgccgatcgt
     1681 caacgagagc ccgttcgtgc tcgtgagagc cgtgacgttg ccgagcactc cgaactcgac
     1741 ggagccgacc tgctgatgca cgacctggcc cgccagcgcg acggcgttcg gaatcgcgaa
     1801 ctgcgcccgc acgctgccgc cggccggcag caggaagtcg aacacgtcgg ggctcgcgag
     1861 cagcgcgcag cccgcgacgc cctgcggcag gatcgacgac agcggcacgg acatggtgga
     1921 caagccgagc acgtcgagcg cgatcgcgtt gcccggcatg ccggtcgcga gccacctgta
     1981 agtggaaccg gtccacggca gcgtggtcgc ggtcagcacg tt
//
LOCUS       DASRDG010000576         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1349473, whole genome shotgun sequence.
ACCESSION   DASRDG010000576 DASRDG010000000
VERSION     DASRDG010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349473"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFA35_09650"
     CDS             complement(<1..180)
                     /locus_tag="VFA35_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT56479.1"
                     /translation="MHARGVSVALGSTLALRDLSFAVPAGWTAIVGPNGAGKSTLLRA
                     LAGLQPPDSGEVMLND"
     gene            complement(246..1259)
                     /locus_tag="VFA35_09655"
     CDS             complement(246..1259)
                     /locus_tag="VFA35_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZT56480.1"
                     /translation="MSDRDRRRRFALGLAFVTAALLLGGLAAGSEGWSLTQLVQTWRS
                     PDGALIVGEIRAPRTLGAWLVGALLGLAGATAQGLFRNPLADPYLLGTASGASLGVVL
                     VLAASAFGGHALSLATADAVARVGLVAAAFAGALLGVLLTLALARGAQQTTRLLLAGV
                     VVGVVLGALGDLVTSAAPDALRGKQAFMLGSTGFLGWGSCGVLALGLVLTLPPSWRLA
                     RALDALTLGTDSARSLGLALPRIRIALVASLALATGVAVSQAGLVAFVGLVAPHLVRR
                     FAPATHGFTLVASATAGGTLLLAADVLARSVIAPQEVPVGALTALLGGGYLIWLLRRG
                     RVS"
     gene            complement(1256..2146)
                     /locus_tag="VFA35_09660"
     CDS             complement(1256..2146)
                     /locus_tag="VFA35_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HZT56481.1"
                     /translation="MSSLLRRFAAGGLVALSTFSTTAPAQLTLRDDRGATLALAAPPQ
                     RIVSLLPSLTESVCALGACARLVGTDRFSNWPASVRALPKLGGLEDAQIERIVSLRPD
                     VVLLSTSARVTDRLEALGLKVLVLDSRDHADVKRSLTTIAQLLGTPAEAERVWAGIER
                     ATHAAIERVPASLRGRRVYFEVDPTPYAAGPGSFIGETLTQLGLANAVPAELGSFPKL
                     NPEYVVRLQPDIVMAVQPSLAEMPKRPGWAALRALQDGRACGFASERYELLVRPGPRM
                     GEAAGAIADCLVGIAQKARQ"
     gene            complement(2156..>3080)
                     /locus_tag="VFA35_09665"
     CDS             complement(2156..>3080)
                     /locus_tag="VFA35_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZT56482.1"
                     /translation="RRDQFTWQNALKLDASNQLMLAYEHLNEQAESSAYLADQKRSNN
                     AFVLGYTGGFGAHVLTADLRRDQNSVYGGETTGRLGWSMEVAKGLRVRALAGTTFRAP
                     SFNELFYPGYGVPTVTPERGRSVELGVVWRAGDSEASATVYRNRVRDLIAFESNVAFC
                     PADPSYSFGCARNIGRAQLQGATLAGAHRFGALRVRATVDFLDAKDVDTGARLTRRAA
                     HQESLAADYEAGAWRFGGALLAVGARPEGAATLGRYTTLDLNARWRFAPQWQLEAKLL
                     NATDRQYEPARGYQALGRQAWIGVRFDSVGL"
BASE COUNT          440 a         1149 c         1136 g          355 t
ORIGIN      
        1 gtcgttcagc atgacctcgc ccgaatcggg cggctgcagg ccggcgagtg cgcgcagcaa
       61 cgtcgacttg ccggcaccgt tcgggccgac gatcgcggtc cagccggccg gaaccgcgaa
      121 gctgaggtcg cgcagcgcca gcgtgctgcc gagcgccacg ctcacgccgc gcgcgtgcag
      181 accggtcact ggagcacctt cgtgctgcgc actccggaat tcgcccttgg ggcggcccgg
      241 cgggctcatg acacccgccc gcgacgcagc agccagatca ggtagccgcc gccgagcagc
      301 gcggtcagcg cgccgaccgg cacctcctgc ggcgcgatca cgctgcgcgc gagcacgtcg
      361 gccgcgagca gcagcgtgcc gccggccgtg gcgctcgcga ccagtgtgaa gccgtgcgtg
      421 gcgggcgcga agcggcgcac caggtgcggt gcgaccaggc cgacgaacgc gaccaggccc
      481 gcctgcgaga ccgccacgcc ggtggcgagc gcgagcgacg cgaccagcgc gatgcggatg
      541 cgcggcagcg ccaggccgag gctgcgcgcg ctgtcggtgc cgagcgtcag cgcgtcgagc
      601 gcgcgcgcga ggcgccagct cggcggcagg gtcaacacga ggccgagcgc gagcacgccg
      661 caactgcccc agccgaggaa gccggtgctg ccgagcatga aggcctgctt gccgcgcagg
      721 gcgtcgggcg cggcgctcgt gaccaggtcg ccgagcgcgc cgagcaccac gccgacgacc
      781 acgccggcga gcagcaggcg cgtcgtctgc tgcgcgccgc gcgcgagcgc gagcgtcagc
      841 agcacgccga gcagcgcgcc ggcgaaggcg gcggcgacca ggccgacgcg cgcgaccgca
      901 tcggccgtcg cgaggctgag cgcgtgcccg ccgaacgcgc tcgcggcgag caccagcacg
      961 acgccgagcg atgcgcccga ggccgtgccg agcaggtacg ggtcggccag cgggttgcgg
     1021 aacaggcctt gcgcggtcgc accggccagg cccagcagcg cgccgaccag ccacgcgccg
     1081 agcgtgcgcg gcgcgcggat ctcgccgacg atcaacgcac cgtcgggact gcgccaggtc
     1141 tgcacgagtt gcgtgagcga ccagccttcg ctgcccgcgg ccagcccgcc gagcagcagc
     1201 gccgcggtga cgaacgccag cccgagcgcg aagcgacggc ggcgatcgcg gtcgctcact
     1261 ggcgagcctt ctgggcgatg cccaccaggc aatcggcgat cgcaccggcc gcctcgccca
     1321 tgcgcggccc cgggcgcacc agcaactcgt agcgctcgct cgcgaagccg caggcgcgcc
     1381 cgtcctgcaa tgcgcgcagc gccgcccagc cgggccgctt cggcatctcg gcgaggctcg
     1441 gttgcaccgc catcacgatg tcgggctgca gccgcacgac gtactccggg ttcagcttcg
     1501 gaaacgaccc gagctcagcc ggcacggcgt tcgcgaggcc gagctgcgtc agcgtctcgc
     1561 cgatgaacga gccggggccg gccgcgtacg gcgtcgggtc gacctcgaag tagacgcgcc
     1621 ggccgcgcag cgacgccggc acgcgttcga tcgccgcgtg cgtcgcgcgc tcgatgccgg
     1681 cccagacgcg ctcggcctcg gcgggcgtgc ccagcagctg cgcgatcgtc gtcaggctgc
     1741 gcttcacgtc ggcatggtcg cgcgaatcga gcaccagcac cttcagcccc agtgcctcga
     1801 ggcgatcggt gacgcgcgcc gaggtcgaga gcagcaccac gtcgggccgc agcgagacga
     1861 tgcgctcgat ctgcgcgtct tccaggccgc cgagcttcgg cagcgcgcgc acgctcgccg
     1921 gccagttcga gaagcgatcg gtgccgacca ggcgcgcgca ggcgccgagc gcgcacacgc
     1981 tctcggtcag cgacggcagc aggctgacga tgcgctgcgg cggcgcggcc aacgcgagcg
     2041 tcgcgccgcg gtcgtcgcgc agcgtgagct gcgccggcgc ggtcgtcgag aacgtcgaga
     2101 gcgccaccag cccgcccgcg gcgaagcgac gaagcaggct gctcatcgcg ggtgctcaaa
     2161 ggccgacgct gtcgaagcgc acgccgatcc aggcctggcg gcccagcgcc tggtagccgc
     2221 gcgcgggttc gtactgccgg tcggtcgcgt tcagcagctt ggcctcgagc tgccactgcg
     2281 gtgcgaagcg ccagcgcgcg ttcaggtcga gcgtcgtgta gcggccgagc gtggccgcgc
     2341 cctcgggccg cgcgccgacc gcgagcagcg caccgccgaa gcgccacgcg ccggcctcgt
     2401 agtcggccgc gaggctctcc tggtgcgcgg cgcggcgggt caggcgcgcg ccggtatcca
     2461 catccttcgc gtcgagaaaa tcgaccgtgg cgcgcacgcg cagcgcgccg aagcggtgtg
     2521 cgcccgccag cgtcgcgccc tgcaattgcg cgcggccaat gttgcgcgcg cagccgaagc
     2581 tgtacgacgg gtcggccggg cagaacgcga cgttgctctc gaacgcgatc aggtcgcgca
     2641 cccggttccg gtagacggtg gccgaggcct cgctgtcgcc agcccgccag acgacgccga
     2701 gctcgacgct gcggccgcgc tcgggcgtca ccgtgggcac gccgtagccc ggatagaaca
     2761 actcgttgaa gctcggcgcg cggaaggtcg ttccggcgag cgcgcgcacg cgcaggcctt
     2821 tcgcgacctc catgctccaa cccaggcgcc cggtggtctc gccgccgtag accgagttct
     2881 ggtcgcggcg cagatcggcc gtcagcacgt gcgcaccgaa cccaccggtg tagccgagca
     2941 cgaacgcgtt gttgctgcgc ttctggtcgg ccaggtacgc gctcgactcg gcttgctcgt
     3001 tcaggtgttc gtaggccagc atcaactggt tcgaggcgtc gagcttcagc gcgttctgcc
     3061 acgtgaactg gtcgcgccgc
//
LOCUS       DASRDG010000577         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1349598, whole genome shotgun sequence.
ACCESSION   DASRDG010000577 DASRDG010000000
VERSION     DASRDG010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349598"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFA35_09670"
     CDS             complement(<1..457)
                     /locus_tag="VFA35_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56483.1"
                     /translation="MNHDDDSWDELLARLAHAEADLGAAEAELQTASPAPLPPGFVDA
                     AVAAARTAPRERRGRTRLFLVPAMLLLAAATVLGLLWPQAHDSSSTMPYALAVQLLLR
                     PDQPPAHRQSAACQVRECLYYGVETLQRLRDDARTPAALRAHARQHLALL"
     gene            complement(468..1085)
                     /locus_tag="VFA35_09675"
     CDS             complement(468..1085)
                     /locus_tag="VFA35_09675"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZT56484.1"
                     /translation="MLPSPTMPADQGHDATTARLLAAGDPDGLRRLLVDHGGRVRGAL
                     QRAFADALDASSIDEALSQASQRAWRAGARFDPARGSLAAWFYVIARNCARRLLECRR
                     RQTALAFVDDLDRAGARPPQRPPDAAPRSSFDQLYRCIDALPPQQRAVILADLRADGT
                     ARTDDLAHQLGTTPNAIYVARANGRKALRRALEALGHAAAAATTP"
     gene            1270..>2032
                     /locus_tag="VFA35_09680"
     CDS             1270..>2032
                     /locus_tag="VFA35_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56485.1"
                     /translation="MFLLEHDIAHCLRSCLHTICRRQWRTLCGEGEPSAAAIVDPEAL
                     VVMSLALRREAGIESMGIDGMMTRWAAVSSRLLNLQRLRSLLHGAPPAAQVGLRWLAE
                     ACAAAGDRRFTALAGPRVRPRHPHGHGPSGNGRAGRVESPAALMLRLRAVFGLGNKAD
                     LLAFALGSTRAGGAQRAITLAAIAQSLVCSIPAARRAAGELVHAGLLHGTRDRPTLYS
                     LQPPAEALAGTGGGAAPWRDWALVMPFLLQALEFAA"
BASE COUNT          236 a          728 c          808 g          260 t
ORIGIN      
        1 cgagcagggc caggtgctgg cgtgcgtgcg cgcgcagcgc cgccggcgtg cgcgcgtcgt
       61 cgcgcaaccg ctgcagcgtc tcgacgccgt agtacaggca ctcgcggacc tggcaggcgg
      121 ccgactgccg gtgcgccggc ggctggtccg gccgcagcag cagctgcacg gccagcgcgt
      181 agggcatcgt cgaagagctg tcgtgtgcct gcggccacag gaggccgagc accgtggcgg
      241 ccgccagcag cagcatcgcg ggcacgagga acaggcgcgt tcgtcccctg cgttctcgcg
      301 gtgcggtgcg cgccgcggcc accgcggcgt cgacgaaccc cggcggcagc ggggccggcg
      361 aagcagtctg cagttccgcc tcggcggcgc cgaggtccgc ctcggcgtgc gcgaggcgcg
      421 ccagcagttc gtcccaggaa tcgtcgtcgt ggttcatggg tcgtggttca tggcgtggtg
      481 gcggcggcgg ccgcgtgccc gagggcctcg agcgcgcggc gcagcgcctt gcggccgttg
      541 gcgcgcgcga cgtagatggc gttgggggtc gtgccgagct ggtgcgcgag gtcgtcggtg
      601 cgggcggtgc cgtcggcgcg caggtcggcg aggatcaccg cgcgctgctg cggcggcagc
      661 gcgtcgatgc agcggtagag ttggtcgaag gacgaccgcg gcgccgcgtc cggtgggcgc
      721 tgcggtgggc gcgcgccggc gcggtcgaga tcgtcgacga acgccagcgc ggtctggcgg
      781 cggcggcact cgagcagccg gcgcgcgcag ttgcgggcga tcacgtagaa ccacgcggcc
      841 agcgagccgc gcgcgggatc gaagcgggcg ccggcgcgcc acgcgcgctg gctcgcctgg
      901 ctcaacgcct cgtcgatcga cgacgcatcg agcgcatccg cgaacgcgcg ctgcagcgcg
      961 ccgcgcacgc gcccgccgtg gtcgaccagc agccggcgca ggccgtcggg atcgccggcc
     1021 gcgagcagcc gcgcggtggt ggcgtcgtgg ccctggtccg cgggcatggt ggggctcggc
     1081 aacacggttc ggtagaggta cccccggacc ttagtccgat gacttcggat cgcgaccggg
     1141 agagatcggt tgctaaggca gcagggtacc gggggcagcg gtcccgcgac aacagcccga
     1201 gcccccatgg atgccgcgaa tcccggatct gccttcgagt tcgccggccc gactccggcg
     1261 gcaccaccca tgttcctgct cgagcacgac atcgcccatt gcctgcgctc ctgtctgcac
     1321 acgatctgcc ggcggcagtg gcgcacgctc tgcggcgaag gcgagccgtc ggcggccgcc
     1381 atcgtcgacc cggaggcatt ggtcgtgatg tcgctggcgc tgcggcggga ggccggcatc
     1441 gagagcatgg gcatcgacgg gatgatgacg cgctgggccg ccgtgagcag ccgcctgctg
     1501 aacctgcagc ggctgcgcag cctcctgcac ggcgcgccgc cggcggcgca ggtgggcctg
     1561 cgctggctcg cggaagcctg cgccgccgcg ggggaccgcc ggttcaccgc gctggcgggg
     1621 ccacgcgtcc gcccgcgcca tccacacggc cacgggccgt ccggcaacgg gcgcgcgggt
     1681 cgcgtggaat cgccggccgc gctgatgctg cgcctgcggg cggtgttcgg gctcggcaac
     1741 aaggccgacc tgctcgcgtt cgcgctgggc agcacgcgcg cgggcggcgc ccagcgcgcg
     1801 atcacgctcg cggcgatcgc gcagtccctc gtgtgctcga tccccgcggc gcggcgcgcg
     1861 gccggcgaac tggtgcatgc ggggctgctg cacggcaccc gcgaccgccc cacgctctat
     1921 tcgctgcaac cgcccgccga agcgctcgcc ggcaccggcg gcggtgcggc gccgtggcgc
     1981 gactgggcgc tcgtcatgcc gttcctgctg caggcgctcg agttcgcggc gg
//
LOCUS       DASRDG010000578         3307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_543689, whole genome shotgun sequence.
ACCESSION   DASRDG010000578 DASRDG010000000
VERSION     DASRDG010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543689"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="VFA35_09685"
     CDS             complement(<1..1494)
                     /locus_tag="VFA35_09685"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZT56486.1"
                     /translation="MHEIELTLQVPAGRGAAVDAAVAGKLPAARLRLQAAYHDTPERA
                     LAAAGLALRLRREGRQWVQTLKGAGDDGLTRAEHNMPRGGGAAMPAIEPALHADTAVG
                     DRLLALLGASPNLQILYRTDIRRRTRALAVRAPGGPAARVELAFDTGHIVAGAAAIEV
                     CELEIELLAGTPLAVIDTARRWLPRFGLWLDTRSKAERGDLLARGEAVAAPRLATTLR
                     LDAALDASAAWQVVLRHCADQILANASQIASGEHGPEHVHQLRVGLRRLRSAIALFEH
                     DDAALAGGAAALFRALGAARDSVVIDAEFGAALAEAMRGAGLALGAAPAAAPPAELPA
                     ARLREGATQAFLLDLLAAMHAEPAAEADPATGAPPLRDLLAARLNRWHRKIVADAKRY
                     AALDDAARHRLRKRAKRLRYATEFCAALFGKREVRRYLKALQALQTRLGAVSDVLVAI
                     TAFAPRAPADAHAVFALGWLAARREALIAAAAPELKAFAKVERFWKHT"
     gene            complement(1566..2075)
                     /locus_tag="VFA35_09690"
     CDS             complement(1566..2075)
                     /locus_tag="VFA35_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HZT56487.1"
                     /translation="MTVRYRRKHWLAALAAWAVAATLLGCDVQRIAELEVGVTTEADV
                     RAKWGEPAAVYTEPDGSKTLEYPRQPAGQVNYMLAIGTDGKLVAMRQVLKPTNFAKIE
                     TGWDKAQVRRLLGLPAKTQRYELKQEETWDWRFNDNGENKLFSVTFDTSGRVTATATT
                     IDPKDSGGR"
     gene            2146..2346
                     /locus_tag="VFA35_09695"
     CDS             2146..2346
                     /locus_tag="VFA35_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HZT56488.1"
                     /translation="MPLFWKAYTSDVTSFIEELKANKPTLEEEQRQGRNLLWDKRIDR
                     EAQAEYRDATVAQQPYVYQTKS"
     gene            2400..3239
                     /locus_tag="VFA35_09700"
     CDS             2400..3239
                     /locus_tag="VFA35_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZT56489.1"
                     /translation="MADAPVGEQDSPAASPAVVDAVALARLYGEPLFAMPTDLYIPPD
                     ALEVFLEAFEGPLDLLLYLIRKQNFNILDIPMASVTRQYLAYVEQIRKTNLELASEYL
                     LMAAMLIEIKSRMLLPPKKSADGQEPEDPRAELVRRLIEYEQMKLAAARIDQLPLIGR
                     DFLRAQVVIEQSLAPRFPDVDADELRAAWADILKRAKLNQHHHITREQLSVREHMSIV
                     LRALQGRRFALFEDLFDPSRGPQVLVVTFIALLELAREHLLEVTQAEAFAPIYVRLAY
                     APA"
BASE COUNT          469 a         1190 c         1175 g          473 t
ORIGIN      
        1 cgtgtgcttc cagaagcgct cgaccttcgc gaacgccttc aactcggggg cggccgcggc
       61 gatcagggcc tcgcgccgcg cggcgagcca gccgagcgcg aacaccgcgt gcgcgtcggc
      121 cggcgcgcgc ggcgcgaagg ccgtgatcgc caccagcacg tcgctgaccg cgccgagccg
      181 cgtctgcagc gcctgcagcg ccttcaggta gcgccgcact tcgcgcttgc cgaacagcgc
      241 ggcgcagaac tcggtggcgt agcgcagccg tttcgcgcgc ttgcgcaggc ggtggcgcgc
      301 ggcgtcgtcg agcgcggcat agcgcttcgc gtcggcgacg atctttcgat gccagcggtt
      361 caggcgcgcg gcgagcagat cgcgcagcgg cggcgccccg gtcgcggggt cggcttctgc
      421 ggcgggttcg gcgtgcatcg cggcgagcag gtcgagcaag aaggcctggg tcgcgccctc
      481 gcgcagccgc gccgccggca actccgcggg cggcgcggcg gcgggcgcgg ccccgagcgc
      541 cagccccgcg ccgcgcatcg cctcggccag cgccgcgccg aactcggcgt cgatgacgac
      601 gctgtcgcgc gccgcgccga gcgcgcggaa cagcgcggcg gcgccaccgg cgagcgccgc
      661 gtcgtcgtgc tcgaacagcg cgatcgcgct gcgcaggcgc cgcaggccga cgcgcagctg
      721 gtgcacgtgc tcgggcccat gctcgcccga ggcgatctgg ctcgcgttcg cgaggatctg
      781 gtcggcgcag tggcgcagca ccacctgcca ggcggccgat gcgtcgagcg ccgcgtcgag
      841 ccgcaacgtg gtcgcgagcc ggggcgctgc caccgcctcg ccacgcgcga gcaggtcgcc
      901 gcgctcggcc ttgctgcgcg tgtcgagcca caggccgaag cgtggcagcc agcggcgcgc
      961 ggtgtcaatc actgcgagcg gcgtgccggc gagcagctcg atctcgagtt cgcagacctc
     1021 gatcgccgcg gcgccggcaa cgatgtggcc ggtgtcgaac gcgagttcga cgcgcgccgc
     1081 aggcccgccc ggtgcgcgca ccgccagcgc acgggtgcgg cggcggatgt cggtgcggta
     1141 gaggatctgc agattcgggc tcgcgccgag caacgcaagc agccgatcgc cgaccgcggt
     1201 gtcggcatgc agcgccggct cgatcgccgg catggccgcg ccgccgccgc gcggcatgtt
     1261 gtgctcggcg cgggtcaggc cgtcgtcgcc ggcgcctttc agcgtctgca cccactgtcg
     1321 gccctcgcgg cgcagccgca gcgcgaggcc ggcggcggcg agcgcgcgct cgggcgtgtc
     1381 gtggtacgcg gcctgcagcc gcagccgcgc ggccggcagc ttgccggcga cggccgcgtc
     1441 gaccgcggcg ccgcgcccag ccggcacctg cagcgtcagt tcgatctcat gcatgccggg
     1501 atcgtgacag gcgacccgcc gaatttgatg aaagcgctgg ccgcagcatc ggcggcgggg
     1561 tcttctcagc gcccgccact gtccttcggg tcgatcgtcg tcgcggtcgc ggtgacgcgg
     1621 ccgctggtgt cgaaggtgac cgagaacagc ttgttctcgc cgttgtcgtt gaagcgccag
     1681 tcccaggtct cttcctgctt cagctcgtag cgctgggtct tggccggcag gccgagcagg
     1741 cggcgcacct gggctttgtc ccagccggtc tcgatcttcg cgaagttggt gggcttgagc
     1801 acctggcgca tcgcgaccag cttgccgtcg gtgccgatcg cgagcatgta gttgacctgc
     1861 ccggccggct ggcgcgggta ttcgagggtc ttgctgccgt cgggctcggt gtagacggca
     1921 gcgggctcgc cccacttggc gcgcacgtcg gcctcggtcg tgacgccgac ctcgagctcg
     1981 gcgatccttt gcacatcgca gccgagcagc gtggcggcga ccgcccacgc ggcgagcgcg
     2041 gccagccaat gcttgcgacg atatcgaacg gtcattccct gtctccccgt taaacttgag
     2101 gccttcactc tagcgcccgc cctgtggcgt gcgcgccaag ccgccatgcc attgttctgg
     2161 aaagcctaca catccgacgt cacctcgttc atcgaggagc tcaaggccaa caagccgacg
     2221 ctcgaggaag aacaacgcca gggccgcaac ctgctctggg acaagcgcat cgaccgcgag
     2281 gcgcaagccg agtaccgcga cgcgacggtg gcccagcagc cttacgtcta ccagaccaag
     2341 agctgaacgc ggccagaccc gtgagcgatg acgtcgtggc ggcacccgag gtgccggaat
     2401 tggcggacgc gccggtcggc gagcaggaca gcccggccgc gagcccggcc gtcgtcgacg
     2461 ccgtcgcctt ggcgcgcctg tacggcgagc cgctgttcgc gatgccgacg gatctctaca
     2521 tcccgccgga cgcgctcgag gtcttcctcg aagccttcga gggcccgctc gacctgctgc
     2581 tgtacctgat ccgcaagcag aacttcaaca tcctcgacat cccgatggcg agcgtgacgc
     2641 ggcagtacct ggcctacgtc gagcagatcc gcaagaccaa tctcgagctc gccagcgaat
     2701 acctgctgat ggcggcgatg ctgatcgaga tcaagtcgcg catgctgctg ccgccgaaga
     2761 agagcgccga cggccaggag cccgaagacc cgcgcgccga gctcgtgcgc cggctgatcg
     2821 agtacgagca gatgaagctc gccgcggcgc gcatcgacca gctgccgctg atcgggcgcg
     2881 acttcctgcg cgcgcaggtg gtgatcgagc agtcgctggc gccgcgtttc ccggatgtcg
     2941 acgccgacga actgcgcgcg gcctgggccg acatcctgaa acgcgcgaag ctcaaccagc
     3001 atcaccacat cacgcgcgag caactcagcg tgcgcgagca catgagcatc gtgctgcgcg
     3061 cgctgcaggg ccggcgcttc gcgctgttcg aagacctgtt cgacccgagc cgtggcccgc
     3121 aagtgctggt cgtgaccttc atcgcgctgc tcgagctggc gcgcgagcac ctgctcgagg
     3181 tgacgcaggc cgaagcgttc gcaccgatct acgtgcggct ggcgtacgcg ccggcttgag
     3241 ccccgaccga ccgcgcctca gcccggcttc acgccgagct tgaacgcgat catcgcgcgc
     3301 agcttgt
//
LOCUS       DASRDG010000579         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1349730, whole genome shotgun sequence.
ACCESSION   DASRDG010000579 DASRDG010000000
VERSION     DASRDG010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349730"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFA35_09705"
     CDS             <1..213
                     /locus_tag="VFA35_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56490.1"
                     /translation="LARAVELAPIAINLLFLGDALWAGGDRDAAAAQWQLASAADGDD
                     ATRWSTPLLRELARRRLAALAREPAK"
     gene            291..>2035
                     /locus_tag="VFA35_09710"
     CDS             291..>2035
                     /locus_tag="VFA35_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZT56491.1"
                     /translation="MPPPKDLEQLLAQEPFVRGLARALVADDADEVVQQTWLQALGSR
                     SSVREPRSWLARTVHNAARNLRRSRRRQLQRERAAAVSERLPSSAELLLREERRRALV
                     LAVDALPAPLRTVVLLRWFEALPPRRIAADLGLPVATVWNQLRRALQLLRERLDAEHC
                     GDRRAWLLPLVPVAAVNGTAALYGGLLVTAKTKAVAAGALVLFLALGLALRAPRGAAL
                     DTTRSAAVDPAVASAPHPALPPVEAPPPEREVVHGAAAATTGALVVHVRHVDSDLPVA
                     QRPVVVGLAGSGRRLEARLVVTDAAGDARIDGLAPGGVYAADLLAGFGIHEQFDVTAG
                     TTVEVTLRVRPGMQIDGVVVDEHGLPIAGALVEYAAAAGGDVRTAAVTDARGGFRLRD
                     CIEYCLVGARAAGRTAAPMRLLMGRRDSTLQVRIELLQAGGQIDGVVVRTDGRPVARA
                     WVCIGPGPFGCLSGDTAVPAIVRSDADGRFTALGLPPGTQPVRVLAAGAAPWEGSCEA
                     VAGATTPLRVVVQDAIACTGVVRGRDRKPIAGVSVEVGESGQETFEYARTESAADGSF
                     TLAGLRSGAMVRARH"
BASE COUNT          225 a          720 c          820 g          270 t
ORIGIN      
        1 ctcgcgcgcg ccgtcgaact ggcaccgatc gcgatcaatc tgctgttcct cggcgacgcg
       61 ctgtgggcgg gcggcgaccg cgacgcggcg gctgcgcagt ggcaactggc gagcgcggcg
      121 gacggcgacg acgcgacgcg ctggtcgacg cctctgttgc gcgaactggc gcgccggcgg
      181 ctcgcggcgc tggcgcgcga accggcgaaa tgacggcggc gaaggaccgt cgcgaaaggg
      241 actggcgggt acaggaaacc gcaggcgcgg tgtcgtttcc gtcgatgacc gtgcctccgc
      301 cgaaggacct ggagcagctg ctggcgcagg agccgttcgt gcgcgggctc gcgcgcgcgc
      361 tcgtcgccga cgacgccgac gaggtcgtgc aacagacgtg gctgcaagcg ctcggcagcc
      421 ggtcttcggt gcgcgagccg cggtcgtggc tggcgcgcac cgtgcacaac gccgcgcgca
      481 acctgcggcg cagccgccgc cggcagctgc agcgcgaacg cgcggcggcg gtgagcgaac
      541 ggctgccgtc gtcggcggag ctgctgctgc gcgaggagcg gcggcgtgcg ctggtgctcg
      601 cggtcgacgc gctgccggcg ccgctgcgga ccgtcgtgct gctgcgctgg ttcgaggcgc
      661 tgccgccgcg ccgcatcgcg gcggatctcg ggctgccggt ggcgacggtc tggaaccagc
      721 tgcgccgcgc gctgcagctc ttgcgcgaac ggctcgacgc cgagcactgc ggcgaccggc
      781 gcgcgtggct gctgccgctg gtgccggtcg cggcggtgaa cggcaccgcg gccctgtacg
      841 gaggtctgct cgtgacggcg aagacgaagg cggtcgcggc gggtgcgttg gtgcttttcc
      901 tggcgctcgg gttggcgttg cgtgcgccgc gcggcgcggc gctggacacg acccggagcg
      961 ccgcggtgga tcccgcggtg gcgagtgctc cgcacccggc gttgccgccc gtcgaggcgc
     1021 ctccgcccga acgcgaagtc gtccacggcg ccgccgccgc gaccaccggc gcgctcgtcg
     1081 tgcacgtccg gcacgtcgat tcggatctgc ccgtcgcgca gcgccccgtg gtggtcggcc
     1141 tcgccggttc cggccggcgg ctcgaggcgc gactggtcgt caccgatgcg gctggcgacg
     1201 cccgcatcga cgggctcgcg ccgggcgggg tctacgctgc cgatctgctc gccggcttcg
     1261 gcatccacga gcagttcgac gtgaccgccg ggacgaccgt cgaggtcacg ctccgggtcc
     1321 ggcccggcat gcagatcgat ggcgtcgtcg tcgacgagca cggcctgccg atcgccggcg
     1381 cgctggtgga gtacgcagcg gcggccgggg gcgacgtgcg gacggctgcc gtcacggatg
     1441 cgcgcggcgg gttccggctg cgcgactgca tcgagtactg cctcgtcggc gcgcgcgccg
     1501 ccggccgcac cgcggcgccg atgcgcctgc tgatgggtcg ccgcgattcg accctgcagg
     1561 tgcgcatcga gctcctgcag gccggcggcc agatcgacgg cgtggtcgtg cggaccgacg
     1621 gacgcccggt ggcccgtgcg tgggtgtgca tcggcccggg tccgttcggc tgtctcagcg
     1681 gcgacacggc cgtgcccgcg atcgtccgca gcgatgccga cggtcggttc acggcgctcg
     1741 gcctcccgcc cggaacgcaa ccggtgcgcg tgctcgccgc gggcgcggcg ccgtgggaag
     1801 gcagctgcga agcagtcgcc ggcgcgacca ctccgctgcg cgtcgtcgtg caggacgcga
     1861 tcgcgtgcac gggcgtggtg cgcgggcgcg accggaagcc gatcgccggg gtcagcgtcg
     1921 aggtcggcga gagcggccag gagaccttcg agtacgcgcg gaccgagtcg gccgcggacg
     1981 ggtcgttcac gctcgcggga ctgcggagcg gggccatggt ccgcgcgcgc cacag
//
LOCUS       DASRDG010000580         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_449640, whole genome shotgun sequence.
ACCESSION   DASRDG010000580 DASRDG010000000
VERSION     DASRDG010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449640"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2182
                     /locus_tag="VFA35_09715"
     CDS             <1..2182
                     /locus_tag="VFA35_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZT56492.1"
                     /translation="SARHMTLRGLFEFVVDPAKAIPIDEVEPAREIVKRFATGAMSLG
                     SISTEAHATLAVAMNRIGGKSNTGEGGEDPARYRAEMRGEQVAEGTHFSDIIGKGDII
                     VDYPLKAGDSLRSKIKQVASGRFGVTTEYLQSADQIQIKMAQGAKPGEGGQLPGGKVS
                     EYIGFLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNANARASVSVKLVSEVGVGT
                     IATGVAKAKADHVVIAGHDGGTGASPWSSIKHAGTPWELGLAETQQTLVLNRLRSRIR
                     VQADGQMKTGRDVVIGALLGADEFGFATAPLVAEGCIMMRKCHLNTCPVGVATQDPVL
                     RKKFTGRPEHVVNFFFFIAEEARSIMAQLGIRKFDDLIGRVDLLDTKKGIEHWKAKGL
                     DFSRVFAQSAAPADVPRRQTEAQDHGLAKALDVKLIEKCRPAIDRGEKVQFMEEVRNV
                     NRTVGAMLSGELIRVRPEGLPDHTIFIQMEGTGGQSFGAFLAKGITIYLIGDANDYTG
                     KGLSGGRIAIRPSIEFRGDATQNIIVGNTVLYGATSGEAFFRGVAGERFAVRLSGATA
                     VVEGTGDHGCEYMTGGTVAVLGKTGRNFAAGMSGGVAYVYDEDGSFASRCNTSMVTLG
                     KVLSAAEQEAEKVHWHNGQPDEVILKKLVEDHHKWTGSLRARHIMDNWVESRAKFVKV
                     FPNEYKRALGERAAKAVMLASESLAVQEADSTIAKAKNKSVPAK"
     gene            2221..>2763
                     /locus_tag="VFA35_09720"
     CDS             2221..>2763
                     /locus_tag="VFA35_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT56493.1"
                     /translation="MGKVTGFMEYERLEEGYAPVAERKQHYKEFVIGLEEADAKVQSA
                     RCMDCGTPFCNNGCPVNNIIPDFNDLVYRAEWKNAFAVLDSTNNFPEFTGRICPAPCE
                     AACTLNVNDDAVGIKSIEHAIIDKAWAEGWVTPRPPKAKTGRRVAVVGSGPAGLAASQ
                     QLARAGHDVTLFEKNDAIGGL"
BASE COUNT          515 a          925 c          952 g          371 t
ORIGIN      
        1 gtcggcgcgc cacatgacgc tgcgcgggct gttcgagttc gttgtcgatc ccgcgaaggc
       61 gattccgatc gacgaggtcg agccggccag ggagatcgtc aagcgcttcg ccaccggcgc
      121 gatgtcgctc ggctcgatct cgaccgaggc gcacgcgacg ctcgcggtgg cgatgaaccg
      181 catcggcggc aagtcgaaca ccggcgaggg cggcgaggac ccggcgcgct accgcgccga
      241 gatgcgcggc gagcaggtcg ccgaaggcac gcacttcagc gacatcatcg gcaagggcga
      301 catcatcgtc gactacccgc tcaaggcggg cgactcgctg cgctcgaaga tcaagcaggt
      361 cgcgtcgggc cgtttcggcg tgaccaccga gtacctgcag agcgccgacc agatccagat
      421 caagatggcg cagggcgcga agcccggcga gggcggccag ctgcccggcg gcaaggtcag
      481 cgagtacatc ggtttcctgc gctactcggt gccgggcgtc gggctgatct cgccgccgcc
      541 gcaccacgac atctactcga tcgaagacct tgcgcagctg atccacgacc tgaagaacgc
      601 gaacgcgcgc gccagcgtca gcgtcaagct ggtcagcgag gtcggcgtcg gaacgatcgc
      661 gaccggtgtg gccaaggcca aggccgacca cgtcgtgatc gccggccacg acggcggcac
      721 cggcgcgtcg ccgtggagct cgatcaagca cgcgggcacg ccgtgggagc tgggcctggc
      781 cgagacgcag cagacgctgg tgctgaaccg gctgcgctcg cgcatccgcg tgcaggccga
      841 cggccagatg aagaccggcc gcgatgtcgt gatcggcgcg ctgctcggtg ccgacgagtt
      901 cggcttcgcg accgcgccgc tggtggccga gggctgcatc atgatgcgca agtgccacct
      961 gaacacctgc ccggtcggcg tcgcgacgca ggacccggtg ctgcgcaaga agttcaccgg
     1021 ccgccccgag cacgtcgtca acttcttctt cttcatcgcc gaggaagcgc gctcgatcat
     1081 ggcgcagctc ggcatccgaa agttcgacga cctgatcggc cgtgtggacc tgctcgatac
     1141 caagaagggc atcgagcact ggaaggcgaa gggcctcgat ttcagccgcg tgttcgcgca
     1201 atccgcggcg ccggccgacg tgccgcgccg ccagaccgag gcgcaggacc acggcctcgc
     1261 gaaggcgctc gacgtgaagc tgatcgagaa gtgccgcccc gcgatcgacc gcggcgagaa
     1321 ggtgcagttc atggaagagg tgcgcaacgt caaccgcacg gtcggcgcga tgctctcggg
     1381 cgagctgatc cgcgtgcgcc ccgagggcct gcccgaccac accatcttca tccagatgga
     1441 aggcaccggc ggccagagct tcggcgcgtt cctggccaag ggcatcacga tctacctgat
     1501 cggcgacgcg aacgactaca ccggcaaggg cctctcgggc gggcgcatcg cgatccggcc
     1561 gagcatcgag ttccgcggcg acgcgaccca gaacatcatc gtcggcaaca ccgtgctgta
     1621 cggcgcgacc agcggcgagg cgttcttccg cggcgtggcc ggcgagcgct tcgcggtgcg
     1681 cttgtcgggc gcgacggcgg tcgtcgaagg caccggtgac cacggctgcg agtacatgac
     1741 cggcggcacg gtcgcagtgc tcggcaagac cgggcgcaac ttcgccgccg gcatgagcgg
     1801 cggcgtggcc tatgtgtacg acgaggacgg cagcttcgcg tcgcgctgca acacctccat
     1861 ggtcacgctc ggcaaggtgc tgagcgcggc cgagcaggag gccgagaaag tgcactggca
     1921 caacggccag cccgacgagg tgatcctgaa gaagctggtc gaggaccacc acaagtggac
     1981 cggctcgctg cgcgcgcgcc acatcatgga caactgggtc gaatcgcgcg ccaagttcgt
     2041 caaggtgttc ccgaacgagt acaagcgtgc gctcggtgag cgcgctgcga aggccgtgat
     2101 gctcgcgagc gaatcgctgg cggtgcagga ggccgacagc acgatcgcca aggcgaagaa
     2161 caagtcggtc ccggccaagt agccgcatcg cctgcaaccc gaacgaacac agaagacatc
     2221 atgggaaaag tcaccggctt catggaatac gagcgcctcg aagagggcta cgcgcccgtc
     2281 gccgagcgca agcagcacta caaggagttc gtcatcggcc tcgaagaagc cgacgcgaag
     2341 gtccagtccg cccgctgcat ggactgcggc acgccgttct gcaacaacgg ctgtccggtc
     2401 aacaacatca ttccggactt caacgacctg gtgtaccgcg ccgagtggaa gaacgcgttc
     2461 gcggtgctcg actcgaccaa caatttcccc gagttcaccg gccgcatctg cccggcgccg
     2521 tgcgaggccg cgtgcacgct caacgtcaac gacgacgcgg tcggcatcaa gagcatcgag
     2581 cacgcgatca tcgacaaggc ctgggccgag ggctgggtca cgccacggcc gccgaaggcg
     2641 aagaccggca gacgcgtcgc cgtcgtcggc tccggcccgg ccggcctggc ggcgtcgcag
     2701 cagctcgcgc gtgccgggca cgacgtgacg ctgttcgaga agaacgacgc gatcggcggc
     2761 ctg
//
LOCUS       DASRDG010000581         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_543735, whole genome shotgun sequence.
ACCESSION   DASRDG010000581 DASRDG010000000
VERSION     DASRDG010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543735"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFA35_09725"
     CDS             complement(<1..568)
                     /locus_tag="VFA35_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56494.1"
                     /translation="MRHWFLAWLGAQLATQLATQLAAQLAAQVPSPLLSGRVVDEQGA
                     PLAGVAVCLLDENPGAPLFVTADLLQEPLARTAADGTWSAALPAAVHGVGMRLLLVAP
                     GRVHVAAPLHSHDGWPVALPRAHTLAGRVVDTDGRPLAGVRVAARDWLCDARYRADNQ
                     RVPWLPEPLTAVLTDDGGRFVLPGTVGAG"
     gene            700..1209
                     /locus_tag="VFA35_09730"
     CDS             700..1209
                     /locus_tag="VFA35_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZT56495.1"
                     /translation="MHVDRQPREDAARIVQQHQAEVWRYLRYLGCSAELADDLTQETF
                     LQYLRAPYEERGATARSGWLRTVARNLYLRSFRQPAVTVRELEEGDAVWLQFARDDGG
                     AGWLDALRSCLQELDGRARAAVRLQYEQRASRQQIGDELGIGQDGVKSLLRRVRGVLR
                     RCIEGRLAS"
     gene            1206..>2145
                     /locus_tag="VFA35_09735"
     CDS             1206..>2145
                     /locus_tag="VFA35_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56496.1"
                     /translation="MSDWQDKVVDAALRQVHGERPPDQTAIVLHRLQTELEPITRLPE
                     AAAAAPALRSRWRTLLGGSALGAAAGLMLTWLVLPRPLPDRGPAARIDVAALDGAAEF
                     AELAAAGAPERSFTLAQGERSAWRAQAGNWLMSRGDRGVELSLAPFGVVSTGTHTRME
                     VRSMELSIRNGAIVTGAMTFAVVSGVVTWKTLSHNETVAAGRELRVEAPKDPGPDAAL
                     ASAQKENQELRDRLQQLETQLARQPAPAAPPAPATKTIDDAAKLPEPPAPAEKGKPAF
                     DNEQFREMLAAIDWNAVGEANKEIAPLLDELAEALNE"
BASE COUNT          305 a          754 c          834 g          252 t
ORIGIN      
        1 caccggcgcc gacggtgccc ggcagcacga agcggccgcc gtcgtcggtg agaaccgcgg
       61 tcagcggttc gggcagccag ggcacgcgct ggttgtcggc gcggtagcgc gcgtcgcaga
      121 gccagtcgcg cgccgcgacg cgtacgccgg cgagcggcct gccgtcggtg tcgacgacgc
      181 ggcccgccaa ggtgtgcgcg cggggcagcg cgaccggcca gccgtcgtgg ctgtgcagcg
      241 gcgcggcgac atgcacgcgg cccggcgcga cgagcagcag ccgcatgccg acgccgtgca
      301 ccgccgccgg cagcgcagcc gaccaggtgc cgtcggccgc ggtccgcgcc agcggctcct
      361 gcaacaggtc cgcggtcacg aacagcggtg cgccggggtt ctcgtcgagc aggcagaccg
      421 cgacgcccgc gagcggcgcg ccctgttcgt cgacgacgcg gccggacagc agcggcgacg
      481 gcacctgcgc cgccagctgt gccgccagct gtgtcgccag ctgtgtcgcc aattgggcgc
      541 cgagccaggc gaggaaccaa tggcgcaatg ggtccgttcg ttcgcggcat cccgccggcg
      601 gcatgggctg catggtagcg gcagttcgcc gtcgcgggag caatcgcgga cccgcgcgca
      661 ccctttcgct ccgcgctgga ttgtggtggg gtgtccgtca tgcacgtcga ccgacagccc
      721 cgcgaggacg ccgcccggat cgtccagcag caccaggccg aggtgtggcg ctacctgcgc
      781 tacctcggct gctcggcaga gctcgccgac gatctcacgc aggagacgtt cctgcagtac
      841 ctgcgcgcgc cgtacgagga acgcggcgca acggcgcgca gcgggtggct gcgcacggtg
      901 gcgcgcaacc tctacctgcg gtcgttccgg cagccggcgg tgacggtgcg cgagctcgag
      961 gaaggcgacg cggtgtggct gcagttcgcg cgcgacgacg gcggcgcggg ctggctcgac
     1021 gcgctgcgca gctgcctgca ggagctcgac ggccgcgcgc gcgccgccgt gcggctgcag
     1081 tacgaacagc gcgcgtcgcg ccagcagatc ggcgacgagc tcggcatcgg ccaggacggc
     1141 gtgaagtcgc tgctgcgccg cgtgcgcggc gtcctgcggc gctgcatcga ggggaggctc
     1201 gcgtcatgag cgactggcag gacaaggtcg tcgacgctgc gctgcggcag gtgcacgggg
     1261 aacggccgcc ggaccagacg gcgatcgtgc tgcaccgcct gcagaccgaa ctggaaccga
     1321 tcacgcgcct gcccgaggcc gcggcggccg cccccgcgct gcgcagccgt tggcggacgc
     1381 tgctcggcgg cagtgcactc ggcgctgccg ccgggctcat gttgacctgg ctcgtgctgc
     1441 cgaggccgct gccggaccgc ggacccgcgg cgcgcatcga cgtggcggcg ctcgacggcg
     1501 cggcggagtt cgccgagctc gcggcggcgg gcgcgccgga gcgctcgttc acgctcgcgc
     1561 agggcgagcg ctctgcttgg cgcgcgcagg cgggcaactg gctgatgagc cgcggcgacc
     1621 gcggcgtcga gctgtcgctg gcgccgttcg gcgtcgtgag caccggcaca cacacacgca
     1681 tggaggttcg ttcgatggaa ttgtcgatca ggaacggggc gatcgtcacc ggtgcgatga
     1741 cgttcgcggt ggtctcggga gtggtgacct ggaaaacgct ctcgcacaac gagaccgtgg
     1801 ccgcaggcag ggagctgcgc gtcgaggcgc cgaaggatcc ggggcccgac gcggcgctcg
     1861 ccagcgcgca gaaggagaac caggagctgc gcgaccgcct gcagcagctc gagacgcagc
     1921 tcgcgcggca accggcgccg gccgcgcccc cggcgccggc gacgaagacc atcgacgatg
     1981 cagcgaagct ccccgagccg ccagctcccg cggagaaggg caagccggcg ttcgacaacg
     2041 agcagttccg ggagatgctc gcggccatcg actggaacgc ggtgggcgag gccaacaagg
     2101 agatcgcgcc gctgctcgac gagctggccg aggcgctgaa cgagg
//
LOCUS       DASRDG010000582         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_543790, whole genome shotgun sequence.
ACCESSION   DASRDG010000582 DASRDG010000000
VERSION     DASRDG010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543790"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFA35_09740"
     CDS             complement(<1..938)
                     /locus_tag="VFA35_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZT56497.1"
                     /translation="MTTPASYDLAPLLAILAVGALLALGPFVWVWRRDHAKGMVARLQ
                     ALTLLTLFLTFDLVLFGAFTRLTDSGLGCPDWPGCYASASPLGAHAEIHAAQAALPDG
                     PVTHGKAWIEMIHRYLATGVGALITLLALLSWVAARNKKLPISPWWASATLVWVCVQG
                     AFGALTVTMKLYPAIVTAHLLGGLTGLALLAIQKEHYRRAPLALPPRLHAAAIVIAVL
                     SVLQIALGGWVSTNYAVLACRDFPTCQGAWWPAMDFEHGFALRRELGAGRDGGFLPFA
                     ALTAIHYVHRLGAYVVLAAMAGFAWALHASFDAALRR"
     gene            complement(952..1629)
                     /locus_tag="VFA35_09745"
     CDS             complement(952..1629)
                     /locus_tag="VFA35_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56498.1"
                     /translation="MHERDSPLRFTVHSMPAPALDDAALRRTQAGRVKMLLVLAICAA
                     PVIASYLSYFLIRPAGRTNYGELIEPQVPIPADLPLTDLQGVAVKSETLKGQWLMVVV
                     SGAACDAICEKQLWVQRQLRETLGRDKDRVDKLWLIDDAGTPKPQTLAGVTTHTDATV
                     LRVPRAALMAWLRPAPGQALEDHIYLVDPLGHWMMRAPANPEPAKLKADMVKLLRASA
                     GWDKAGR"
     gene            complement(1616..2371)
                     /locus_tag="VFA35_09750"
     CDS             complement(1616..2371)
                     /locus_tag="VFA35_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HZT56499.1"
                     /translation="MALSAKGRAAVMLIATLIGVGVTANLGAWQLRRAAQKIALQDAL
                     ESRARLGALTASEIAGDLARTPDQVEAQHYRPVHLRGRWLAQHNVFLENRQMNGRVGF
                     YLVTPLQLDGRPEVVLVQRGWLPRDLRERTLLPPVATPAGTVEVDGHVAPAPARLYEF
                     APSTAGLIRQNLDLDEFRRETGLPLAPVSVLQADSPGNAGDGLLRQWPHPAVDVQKHY
                     GYAFQWFALGALMAGLYVWFQLVRPRWQRDARA"
     gene            2402..>2614
                     /locus_tag="VFA35_09755"
     CDS             2402..>2614
                     /locus_tag="VFA35_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HZT56500.1"
                     /translation="MKYLIGLAFVGILGALALAGVFMLRDGRDGETKRGNMMRALAVR
                     VGLSVLLFACVLFSWWAGWIQPTGIPL"
BASE COUNT          395 a          911 c          954 g          354 t
ORIGIN      
        1 cggcgcagcg cggcgtcaaa gctggcgtgc agcgcccacg cgaagccggc catcgcggcc
       61 agcacgacat aggcaccgag gcgatggacg tagtggatcg ccgtcagcgc cgcgaacggc
      121 aggaagccgc cgtcgcggcc cgcgccgagt tcgcgccgca gcgcgaagcc gtgctcgaag
      181 tccatcgccg gccaccacgc gccctggcag gtcgggaagt cgcggcaggc cagcaccgcg
      241 taattggtgc tgacccagcc gccgagcgcg atctgcagca cgctgagcac ggcgatgacg
      301 atcgcggccg cgtgcaggcg cggcggcagc gcgagcggcg cgcggcggta gtgctctttc
      361 tggatcgcca gcagcgccag cccggtgagg ccgccgagca ggtgcgcggt gacgatcgcc
      421 gggtaaagct tcatcgtcac cgtcagcgcg ccgaacgcgc cctgcacgca gacccagacc
      481 agcgtcgcgc tcgcccacca cggcgagatc ggcagcttct tgttgcgcgc ggcgacccag
      541 ctcagcagcg caagcagcgt gatcagcgca ccgacgccgg tcgcgaggta gcggtggatc
      601 atctcgatcc aggccttgcc gtgcgtgacc gggccgtccg gcagcgcggc ctgcgccgcg
      661 tggatctcgg cgtgcgcgcc gagcgggctc gcgctcgcgt agcaaccggg ccagtcgggg
      721 cagccgaggc cggaatcggt caggcgcgtg aacgcgccga acagcaccag gtcgaaggtg
      781 aggaagaggg tgagaagcgt cagcgcctgc agccgcgcga ccatgccctt ggcgtgatcg
      841 cgacgccaga cccagacgaa cgggccgagc gcaagcagtg cgccgaccgc gaggatcgcc
      901 agcagcggtg ccagatcata ggaagcaggt gtcgtcatcg ccagcgcgtg ctcagcgccc
      961 cgccttgtcc cagcccgccg aagcgcgcag cagcttgacc atatcggcct tcagcttcgc
     1021 gggttcgggg ttcgccggcg cacgcatcat ccagtggccg agcgggtcga cgaggtagat
     1081 gtggtcttcg agcgcctggc cgggcgccgg ccgcagccat gccatcagcg cggcgcgcgg
     1141 gacgcgcagc acggtcgcgt cggtgtgggt cgtgacgccg gcgagcgtct gcggcttcgg
     1201 cgtgccggcg tcatcgatca accagagctt gtcgacgcgg tccttgtcgc ggccgagcgt
     1261 ctcgcgcagc tggcgctgca cccacagctg cttctcgcag atcgcgtcgc aggccgcgcc
     1321 cgagacgacg accatcagcc actggccctt cagcgtctcg gacttgaccg ccacgccctg
     1381 caggtcggtc agcggcaagt cggccgggat cggcacctgc ggctcgatca gctcgccgta
     1441 gttggtgcgg cccgcgggcc ggatcaagaa gtagctcagg tacgacgcga tcaccggcgc
     1501 ggcgcagatc gccagcacga gcagcatctt cacgcggccg gcctgggtgc ggcgcagcgc
     1561 cgcgtcgtcg agcgccggcg ccggcatcga gtgcaccgtg aagcgcagcg gcgaatcacg
     1621 ctcgtgcatc gcgttgccag cggggccgga cgagttggaa ccagacatac agacctgcca
     1681 tcagcgcacc cagcgcgaac cactggaacg cgtaaccgta gtgcttttga acatcgaccg
     1741 cggggtgcgg ccattggcgc agcaggccgt cgcccgcgtt gcccggggaa tcggcctgca
     1801 gcaccgacac gggcgcgagc ggcaggccgg tttcgcgcct gaactcgtcg aggtcgagat
     1861 tttgccggat caggccggcg gtcgacggcg cgaactcata gagccgcgcc ggcgccgggg
     1921 cgacgtggcc gtcgacctcg accgtgccgg cgggtgtggc gaccggcggc agcagcgtgc
     1981 gctcgcgcag gtcgcgcggc aaccagccgc gctgcaccag cacgacctcg ggccggccgt
     2041 cgagctgcag cggcgtgacg aggtagaagc cgacgcggcc gttcatctgc cggttctcga
     2101 ggaagacgtt gtgctgcgcc agccaccggc cgcgcaggtg caccgggcgg tagtgctgcg
     2161 cttccacctg atcgggcgtg cgcgcgagat cgccggcgat ctctgacgcg gtgagcgcgc
     2221 ccagccgcgc gcgcgactcg agcgcgtcct gcagcgcgat cttctgcgcg gcgcggcgca
     2281 actgccacgc gccgaggttc gcggtcacgc ccacaccgat caaggtggcg atcagcatga
     2341 ccgcggcgcg gccctttgcg ctcaatgcca tggccgggcg cgcggcggat aatcggcgcg
     2401 catgaagtac ctgatcggcc tcgccttcgt cggcatcctg ggcgcgctgg cgctggccgg
     2461 cgtgttcatg ctgcgcgacg gccgcgacgg cgagaccaag cgcggcaaca tgatgcgtgc
     2521 gctcgcggtt cgcgtcggct tgtcggtgct gctgttcgca tgcgtgctgt tcagttggtg
     2581 ggcgggctgg attcagccga ccggcatccc gctc
//
LOCUS       DASRDG010000583         4660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_449833, whole genome shotgun sequence.
ACCESSION   DASRDG010000583 DASRDG010000000
VERSION     DASRDG010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449833"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VFA35_09760"
     CDS             complement(<1..889)
                     /locus_tag="VFA35_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZT56501.1"
                     /translation="MTEPAASTGPLAAWASDPRRTRGRRHAEAPAPTRNDFQRDRDRI
                     VHSTAFRRLVYKTQVFLNHEGDLFRTRLTHSIEVAQLARSIARTMRLNEDLCEAIALA
                     HDLGHTPFGHAGQDALDERVRQADPDSHGFEHNFQSLRVVDVLEERYPAYDGLNLTFE
                     TREGILKHCSRRRALALEIDEPGGVGRRFLDRSVPSLEAQVCNLADEIAYNVHDIDDG
                     VRSSLLVYEQLLELPLFLRFHALALQGAPALTGRRLLYETLRRMLSALVYDMVDASTA
                     LIEAHGPASADAARAGPPLI"
     gene            complement(886..1977)
                     /gene="aroB"
                     /locus_tag="VFA35_09765"
     CDS             complement(886..1977)
                     /gene="aroB"
                     /locus_tag="VFA35_09765"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZT56502.1"
                     /translation="MTTDIERVHIELAERGYDILIGTGLLDRPDTYRDLPVGAAAVIV
                     SNETVAPLLAEALARALSGRFARVQLVVLPDGEAHKDWPALNSIFDALLASGCDRKTV
                     LFALGGGVVGDMTGFAAACYMRGVPYVQVPTTLLAQVDSSVGGKTAINHPLGKNMIGV
                     FNQPLRVVADLGTLDTLPQRELIAGLAEVIKYGPIADAAFFDWIDANLDALKARDKGV
                     LAHAIRRSCEIKAWVVAQDERENDLRAILNFGHTFGHAIETGLGYGEWLHGEAVGCGM
                     VMAADLSARLGLIDAAYAGRIRAVIERAGLPVVGPRLGASRYLELMRLDKKAEAGEIR
                     FVVIEAPGRAALRRAPDALVAQVLDAHTE"
     gene            complement(2016..2585)
                     /locus_tag="VFA35_09770"
     CDS             complement(2016..2585)
                     /locus_tag="VFA35_09770"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZT56503.1"
                     /translation="MARSGNIALVSMPGGGKSTIGRHLARRLNREFADADVVVERRIG
                     CSIREFFEREGEARFRDIEQEVIAELLSATNLVLATGGGVVLRETNRESLHARAAVVY
                     LRSTPEDLYRRLRYDTKRPLLQVPDPLARLREMYALRDPLYREVAHFHVDTGRPSVPT
                     LVNMIMMQLELGGAIDAADAPSPIDTPPG"
     gene            complement(2612..3028)
                     /locus_tag="VFA35_09775"
     CDS             complement(2612..3028)
                     /locus_tag="VFA35_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56504.1"
                     /translation="MKHTRIYASIFMVAAAVSLASCGGGGDAEAGSPTAFSVQPTTFT
                     FTAFPPSLGGPPAGLCASTDAGEYFVYGGAAPYRLDNPNPDSVVLDRTTVSERGGSFK
                     VRFVGPGCVSPALIIIVDKLDNQVVLTLNNKPSGGT"
     gene            complement(3074..>4660)
                     /locus_tag="VFA35_09780"
     CDS             complement(3074..>4660)
                     /locus_tag="VFA35_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ family protein"
                     /protein_id="HZT56505.1"
                     /translation="RQQGQSLVVEFLRSTLPETLRRRLDVTDFGTPVQSVTTTQNGDR
                     VRMLVEPRGNWEHSAYQSDNQFVLEVRPQKVDPNKLTQGRGYAGEKLSLNFQNIEVRA
                     LLQVIADFTNFNVVTSDTVTGNVTLRLKDVPWDQALDIILQAKGLGLRKSGNVILIAP
                     KDELNAKEKVELEAKQQIAALEPLRTQSFQLNYAKAADVATGLTGQASGQGGGGTASR
                     ILSARGSVIYETRTNQLFVSDVPSKLQEIQDMIAKIDIPVRQVLIESRIVIADDTFGR
                     SLGVKLGLVDQRGARGGISGYNVGGSNYIGVGGNLNAVGSQTQQNGAASSFSDTNFVS
                     LPATPLGGNSAASIALSLFTAGANRFVNLELSALEADGKGKIVSSPRVITADQQKALI
                     EQGEEIPYQTTSLSGTQTQFRKANLKLEVTPQITPEGSVILDVDITKDSRGASAGQAG
                     FAINTKHVQTKVLVENGGTVVIGGIFEQIDREDVSKVPFLGDVPYLGNLFKTRTKSSS
                     KTEMLIFLTPKVVTDRSAAR"
BASE COUNT          748 a         1544 c         1550 g          818 t
ORIGIN      
        1 cgatcagcgg tgggccagcg cgtgcggcat cggccgatgc cgggccgtgc gcctcgatca
       61 gcgccgtcga cgcgtcgacc atgtcgtaga ccagcgccga gagcatgcgc ctgagcgttt
      121 cgtacagcag gcgccgaccg gtcagagccg gcgcgccttg cagggccagt gcgtgaaagc
      181 gcaagaacag cggcagctcg agcagttgct cgtagaccag caggctcgag cgaacgccat
      241 cgtcgatgtc gtgcacgttg taggcgatct cgtcggcgag gttgcagacc tgcgcttcga
      301 ggctcggcac gctgcgatcg agaaagcggc gcccgacgcc gcccggctcg tcgatctcga
      361 gcgccagcgc gcggcgccgc gagcagtgct tgaggatgcc ctcgcgcgtt tcgaaggtca
      421 ggttgagccc gtcgtaggcg gggtagcgct cctcgagcac gtcgaccacg cgcaggctct
      481 gaaagttgtg ctcgaacccg tggctgtccg gatcggcctg gcgcacgcgc tcgtccagcg
      541 catcctgccc ggcgtggccg aacggcgtgt ggcccaggtc atgggcgagc gcgatcgcct
      601 cgcacagatc ctcgttcagc cgcatcgtgc gagcgatcga gcgcgcgagc tgtgcaactt
      661 cgatcgagtg cgtcaggcgc gtgcggaaca ggtcgccttc gtgattcagg aagacctggg
      721 tcttgtagac caggcgccga aacgccgtgc tgtggacgat gcggtcgcgg tcgcgctgaa
      781 agtcgttgcg cgtcggcgcc ggcgcttcgg cgtgtcggcg cccgcgggtg cggcgcgggt
      841 cgctggccca agcagccagc ggcccggtcg acgcagccgg ctcggtcatt cggtgtgggc
      901 gtcgagcacc tgcgccacca gcgcgtccgg cgcacggcgc agtgcggcgc ggccgggcgc
      961 ctcgatcacg acgaaccgga tctcgccggc ttcggccttc ttgtccaaac gcatcagctc
     1021 gaggtagcgc gaggcgccca ggcgcgggcc caccaccggc agacccgcgc gctcgatcac
     1081 ggcgcgaatg cgaccggcgt aggcggcatc gatcaggccg agccgcgccg acaggtcggc
     1141 cgccatcacc atgccgcaac cgaccgcctc gccgtgcagc cattcgccgt agcccagccc
     1201 ggtttcgatc gcatgaccga aggtgtggcc gaaattcagg atcgcgcgca gatcgttctc
     1261 gcgctcgtct tgagcgacca cccatgcctt gatctcgcag gatcggcgga tcgcatgtgc
     1321 gagcacgccc ttgtcgcgcg ccttcaaggc gtcgaggttc gcatcgatcc agtcgaagaa
     1381 tgcggcatcg gcgatcggac cgtacttgat gacctcggcc agcccggcga tcagttcgcg
     1441 ctgcggcagc gtgtcgagcg tgccaaggtc ggcgacgaca cgcagcggct ggttgaacac
     1501 gccgatcatg ttcttgccga gcgggtggtt gatcgcggtc ttgccgccga ccgatgagtc
     1561 gacctgcgcc agcagcgtcg tcggcacctg cacgtaaggc acgccgcgca tgtagcacgc
     1621 cgccgcgaac ccggtcatgt cgcccacaac gcctccgccg agcgcgaaca acacggtctt
     1681 gcggtcgcac cccgacgcga gcagcgcatc gaagatgctg ttcaacgccg gccaatcctt
     1741 gtgcgcctct ccgtcgggca gcacgacgag ttgcacgcgc gcgaacctgc cgctcagcgc
     1801 ccgcgccagc gcttcagcga gcagcggcgc gacggtctcg ttgctcacga tcaccgctgc
     1861 cgcgcccaca ggcaggtcgc ggtaggtgtc ggggcggtcc agcaggccgg tgccgatcaa
     1921 gatgtcgtag cctcgctcgg ccagctcgat gtgcacacgt tcgatgtcgg tcgtcatcac
     1981 cgaagtgtac gagtgcgcca cgctcggcac gcgtgtcaac cgggcggcgt gtcgatcggc
     2041 gacggcgcgt ctgcggcgtc gatcgcgccg ccgagttcca actgcatcat gatcatgttg
     2101 accagcgtcg gcaccgatgg gcggccggta tcgacatgga agtgtgcgac ctctcggtac
     2161 aacgggtcgc gcagcgcata catctcgcgc agccgcgcga gcgggtcggg cacttgcagc
     2221 aagggccgct tcgtgtcgta gcgcaaacgg cgatacaaat cctcgggtgt cgagcgcagg
     2281 tagacgacgg cggcgcgcgc gtgcagcgac tcgcggttcg tctcgcgcag cacgacacca
     2341 ccgccggtcg cgagcaccaa attcgtcgcg ctcagaagct cggcaatcac ctcctgctcg
     2401 atgtcgcgaa accgggcctc gccctcgcgc tcgaagaact cgcggatcga gcagccgatg
     2461 cggcgctcga ccaccacatc ggcgtcggcg aactcgcggt tcagccgccg cgcgaggtgt
     2521 cggccgatgg tcgacttgcc ccccccgggc atcgagacca gggcgatgtt gccactgcgg
     2581 gccacgaacg cgtgctaccg atgcgcggtc ttcaagtgcc gccggacggc ttgttgttga
     2641 gcgtcagcac gacctggttg tcgagcttgt cgacgatgat gatcagcgca ggtgaaacgc
     2701 agcccggacc gacgaaacgc accttgaaac tgccgccgcg ctcagaaaca gtcgtccggt
     2761 cgagaacaac cgaatcaggg ttcgggttgt ccagcctgta aggggcagcg cctccgtaca
     2821 cgaagtactc cccagcgtcc gtagacgcgc acagccccgc gggcggcccc ccgagcgacg
     2881 gaggaaaggc ggtgaacgta aacgtcgtcg gctggacgct gaatgcggtc ggcgagccgg
     2941 cctcggcgtc gcccccgccg ccgcaactgg cgagcgagac ggccgcggcc accatgaaga
     3001 tacttgcgta gattctcgtg tgcttcatct aggacacctg ttcagtctcg gccatgcgtg
     3061 ttcttgtggg atgtcagcgt gccgccgacc ggtccgtcac cactttcggg gtcaggaaga
     3121 tcagcatttc tgtcttgctg ctcgacttgg tgcgggtctt gaagaggttg ccgaggtaag
     3181 gcacatcgcc gaggaaaggc accttcgaca cgtcttcccg gtcgatctgc tcgaagatgc
     3241 cgccaatcac gacggtgcca ccattctcga ccagcacctt ggtctgcacg tgcttggtgt
     3301 tgatcgcgaa gccggcctgc cccgcggacg cgccacggct gtcctttgta atatcgacgt
     3361 cgaggatcac actgccttcc ggcgtgattt gcggggtcac ttcgagtttg aggttggctt
     3421 tgcggaactg ggtctgagtg cccgacagag acgtggtctg gtacgggatc tcctcccctt
     3481 gctcaatcag cgccttctgc tggtccgcgg tgatgacgcg cgggctcgac acgatcttgc
     3541 ccttgccgtc ggcttcgagg gccgagagct caaggttcac gaaccggttg gcacccgcgg
     3601 tgaacaagga aagtgcgatg cttgccgcac tgttgccgcc cagtggagtt gcgggcaagc
     3661 tgacgaaatt cgtgtcgctg aagctgctgg cggcgccgtt ttgctgcgtc tgcgagccga
     3721 cggcattcag gttgccgccg acaccgatgt agttgctgcc gccgacgttg tagcccgaga
     3781 tgccgccgcg cgcaccgcgc tgatcgacca agccgagctt gacgccgagc gagcgcccga
     3841 acgtatcgtc ggcgatcacg atgcgcgact cgatcagcac ttggcgcacc gggatgtcga
     3901 tcttcgcgat catgtcctgg atttcctgca gcttcgacgg cacgtcgctg acgaacaact
     3961 ggttcgtgcg cgtttcgtag atcacgctgc cgcgtgccga caggatgcgc gacgcggttc
     4021 cgccgccgcc ctgcccggaa gcctgcccgg tcagcccggt cgcgacgtcg gctgctttcg
     4081 catagttgag ttggaacgac tgcgtacgca gcggctcgag cgcagcgatc tgctgcttgg
     4141 cttcgagctc gaccttctcc ttcgcattga gttcgtcctt cggcgcgatc agtatcacgt
     4201 tgcccgactt gcgcagcccg aggcccttgg cctgcaggat gatgtcgagc gcctgatccc
     4261 agggcacgtc cttcaggcgc agcgtcacgt tgccggtgac ggtgtcgctg gtgacgacgt
     4321 tgaagttggt gaagtcggcg atcacctgca gcagcgcacg cacctcgatg ttctggaagt
     4381 tgagcgagag tttctcgccg gcatagcccc gaccctgcgt gagcttgttc gggtcgacct
     4441 tctgcgggcg cacttcgagc acgaactggt tgtcgctctg gtacgcgctg tgctcccagt
     4501 tgccacgcgg ctcgaccagc atgcggacgc gatcgccgtt ctgcgtcgtc gtgacgctct
     4561 gcaccggcgt accgaagtcg gtcacgtcca gacggcgccg cagcgtttcc ggcagggtcg
     4621 aacgcaggaa ctcgaccacc aggctttggc cctgctggcg
//
LOCUS       DASRDG010000584         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1122668, whole genome shotgun sequence.
ACCESSION   DASRDG010000584 DASRDG010000000
VERSION     DASRDG010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122668"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFA35_09785"
     CDS             complement(<1..310)
                     /locus_tag="VFA35_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZT56506.1"
                     /translation="MKNIHTTAIALAVAAVLGACSPAKPPAEAPRPVRTAEIRYDATR
                     EANRYAGSVQSRFEVDQAFRVGGKVALRKVDVGQLVHEGDVLAVLDDSDLRLAEEVAR
                     Q"
     gene            complement(338..1909)
                     /locus_tag="VFA35_09790"
     CDS             complement(338..1909)
                     /locus_tag="VFA35_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZT56507.1"
                     /translation="MRTGPYADITSNPHEIHPMPRLPTTCPLIRDVRQTTFRHPERLT
                     SLIVAAALAASIALVFTGCSTPVMKPALDVQTTFAASSASEMEPEAAWWETFHDPVLS
                     NLVQRAAQENRDIKIAAERLRAARSGVTISRSFLAPSVSAVGSASDRSSGYGDATKQL
                     LPDTRTGSAGLEVAWEVDLSGRLRAGAAAATAEALAAEDGVRGVRLLVMTDVTTNYFT
                     LVGALRQLDTLRAISAAHDETLRLVQARYRAGLATPFDVERAQTDAESARAQIPPLET
                     IAAVSRHRIAVLIGDQAFHGASIEPWRGDLAVADIQPGQPAALLQRRPDVLALMAQLD
                     AANARRKEAAAEWFPRLFLGATFGGQNVGLNGVALGAARFTNVAAMLAMPIFNAGRTS
                     ALNKIAESGQQEALLRAEDGMVRALEDVENALVSLASDRQRSKSLQAASASAEAALGR
                     AQSLYDRGQIDLLPLLDAQRTRLAARISANDGSTRLLLDSVQLYKALGGGWQVFEPTG
                     PFQAQPTAVVSASHF"
     gene            complement(1977..2192)
                     /locus_tag="VFA35_09795"
     CDS             complement(1977..2192)
                     /locus_tag="VFA35_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56508.1"
                     /translation="MVLNPESKYPYRRAYVVNLRGDATPAALAGRIENMVTGRQHEFA
                     SAQELLASILSDLASACGEHAADVAGE"
     gene            2360..>3321
                     /locus_tag="VFA35_09800"
     CDS             2360..>3321
                     /locus_tag="VFA35_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZT56509.1"
                     /translation="MKLKLTPGRLLAAAIVVLAFWIVHGFTQALLAAGVIAIASWPLY
                     LAFRARLPRSVGQSSGAAIFTVAITVFLLAPMVFACGALLSEAHALLHGVAAADRNGL
                     PDWLAKAPVFGPSLAAQWEQRVVLPDALSTLTQHADPGALLGWARSLGQFTFHHALTL
                     SFTVLLLGFLYQIGGSLARELTAVLRHAIGDRAERYVDVGTRAVRSSVNSMLVVGMFD
                     ALATALAYAVAGAPHPLLWAAITGALAAVPFLGYAAVAAMALELAMQGASTAGLLALM
                     LGCAVLLCGDKLVRPMVARGGVRLPFVWVLMGCIGGFGVLGLAGL"
BASE COUNT          467 a         1112 c         1186 g          556 t
ORIGIN      
        1 gctggcgcgc gacctcctcg gcgagccgga ggtcggaatc gtcgagcacg gccaggacgt
       61 cgccctcgtg cacgagttgg ccgacgtcga ccttgcgcag cgccaccttg ccgcccacgc
      121 ggaaggcctg gtcgacctcg aagcgcgact gcacgctgcc ggcatagcga ttggcttcgc
      181 gggtggcgtc atagcggatt tcggccgtgc gcacgggtcg cggcgcctcg gcgggaggct
      241 tggccgggct gcacgcgccg agcacggcag caacggccaa tgcgatggcc gtggtgtgga
      301 tgttcttcaa ggcagttcct cgtgtagggt gatgggttca gaaatgcgac gcggaaacaa
      361 cggccgtagg ctgggcctgg aatggacccg ttggctcgaa cacctgccag ccgccgccca
      421 gcgccttgta gagctgcacg ctgtcgagca gcaggcgggt gctgccgtcg ttggcggaga
      481 tgcgtgccgc gaggcgcgtg cgttgcgcat cgaggagcgg tagcaggtcg atctgtccgc
      541 ggtcgtagag cgattgcgcc cgccccagcg cggcttcggc ggaagcggac gcagcctgaa
      601 gcgatttcga acgctgccga tccgaagcga gcgagacgag cgcgttctcg acgtcttcaa
      661 gtgcgcgcac catgccgtct tcggcgcgca gaagagcctc ttgctggccg ctctcggcga
      721 tcttgttcag agcgctcgtg cggccggcgt tgaagatcgg catcgcaagc atcgccgcca
      781 cgttggtgaa gcgcgcggcg ccaagggcca cgccgttcag ccccacgttc tggcctccga
      841 acgtcgcgcc gagaaacagg cgcggaaacc attcggccgc ggcctccttg cggcgcgcat
      901 tggcggcgtc cagctgtgcc atcagcgcca gcacgtcggg ccgccgctga agcagcgcgg
      961 cgggctgtcc gggctggatg tcagcgacgg ccaggtcgcc gcgccatggc tcgatgctcg
     1021 cgccgtgaaa ggcctggtcg ccgatcagca ccgcgatgcg gtgccgcgac actgccgcaa
     1081 tcgtctcgag cggcgggatc tgcgcgcgcg ccgactcagc atcggtctgc gcgcgttcca
     1141 catcgaaggg cgtcgccagc cctgcccgat agcgcgcctg caccaggcgc aaggtctcgt
     1201 cgtgagcggc ggagatggcg cgcagggtgt caagctggcg cagtgcgccg accagcgtga
     1261 agtagttggt cgtgacatcg gtcatcacga gcaggcgcac accacgcacg ccatcctccg
     1321 cggccagcgc ctcggcggtc gctgccgccg ctcctgcgcg caggcgcccg ctcaggtcga
     1381 cctcccaggc aacctccagt ccggcgctgc cagtccgggt gtccggcagc agctgcttgg
     1441 tcgcgtcgcc gtagccgctg ctgcggtcgc tggccgagcc gaccgcgctg acgctcggcg
     1501 ccaggaagga acggctgatc gtcactccgg atcgcgccgc gcgcagccgc tcggcggcga
     1561 tcttgatgtc gcggttctct tgcgccgcgc gctgcaccag gttgctcagg accgggtcgt
     1621 ggaaggtttc ccaccaggcg gcctcgggct ccatctcgga ggccgagctc gcggcgaaag
     1681 tcgtctgcac gtccagcgcg ggcttcatta ccggcgtgga acagccggtg aagacgaggg
     1741 cgatcgacgc cgccagcgca gcggcgacga tgagacttgt gaggcgctcg gggtgtcgga
     1801 aggtcgtctg cctgacatcg cggatcagcg gacaagtggt tggaagtcgc ggcatgggat
     1861 ggatctcgtg tggattcgag gtgatgtcag cgtagggccc ggtgcgcacc gagtcgtcag
     1921 cgttgggtcg cgattcggtc gacgttccga gctctcactc attcattcgc gcggcgtcat
     1981 tcgccagcga cgtctgccgc gtgctcgccg cacgccgacg cgaggtcgga caagatcgac
     2041 gccagcagct cttgggcgga cgcgaactcg tgctggcgtc cggtgaccat gttctcgatg
     2101 cgacccgcaa gggccgccgg cgtggcgtcg ccccgcagat tcacgacata cgcacggcgg
     2161 tacgggtact tggattcagg gttgaggacc atcgcagacc aaggaaagga agactgggcc
     2221 acagcgtagg cggccccctt ggcggagtcg tcagcgaaag gtcggccttc agtcgcgaca
     2281 aggctcgcgg caacgccgac ggaatcgcga ccggtcgctg acgactgagg gcgcaagcgt
     2341 ccctagcatg gtggacacca tgaaactcaa gctcaccccg ggacggctac tcgccgccgc
     2401 gatcgtggtc ctggccttct ggatcgtgca cggcttcacc caggccttgc tggcggccgg
     2461 cgtcatcgcc atcgcaagct ggcctctcta cctcgccttc cgcgcgcggc tgccgcgcag
     2521 cgtgggccag agcagcggcg cggcgatctt cacggtggcg atcacggtgt tcctgctcgc
     2581 gccgatggtg ttcgcctgcg gggcgctgct cagtgaagcg catgcgctgc tgcatggcgt
     2641 ggccgccgcc gaccgcaatg gtctgccgga ctggctggcc aaggcgcccg tgttcgggcc
     2701 ttcgctggcc gcgcaatggg aacagcgggt cgttctcccc gacgccctct cgacgctgac
     2761 gcagcacgcg gaccctggcg ccctcctcgg ctgggcacga tccctgggac agttcacctt
     2821 ccaccatgcg ctgaccctca gcttcactgt cctgctgctg ggctttctct atcaaatagg
     2881 gggatcgctc gcccgcgagc tcaccgccgt cctgcggcac gccatcggcg accgagctga
     2941 acgctatgtc gatgtgggca cgcgcgcggt gcgctcctcg gtgaacagca tgctggtggt
     3001 gggcatgttc gatgcgctcg ctacggccct cgcgtatgcg gtggccggcg cacctcaccc
     3061 gctgctctgg gccgcgatca ccggcgcgct ggccgcggtg ccgttcctgg gctacgcggc
     3121 cgtggctgcg atggcgctgg agctcgcaat gcagggcgcg tccaccgccg ggctgctggc
     3181 gctgatgctg ggttgcgcgg tgctcctttg cggcgacaag ctggtgcggc cgatggtggc
     3241 gcgcggcggg gtgcggctgc ccttcgtctg ggtgctgatg ggttgcatcg gcggcttcgg
     3301 cgtgctcggg ctggcgggcc t
//
LOCUS       DASRDG010000585         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_544675, whole genome shotgun sequence.
ACCESSION   DASRDG010000585 DASRDG010000000
VERSION     DASRDG010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544675"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VFA35_09805"
     CDS             complement(<1..1118)
                     /locus_tag="VFA35_09805"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT56510.1"
                     /translation="MTSYLDRELGLKVAAALAALPVVVISGLRQSGKTTFLQHDARLR
                     DRRYVTLDDWPVLEQARRDPDAFVAGDEPLTVDEAQRCPELLLAVKRIVDRRRRPGQI
                     VLSGSANFRLLRSVGESLAGRAVYLPMHPLTRRERARATQIEPWLAQLFAAGELPRRR
                     EFTAVTDEEVLRGGMPQLALDPALDRDLWLRGYEQSYLERDVRDLAQVADLAAFRRVL
                     RLTALRSGQLGNESGIGRDAKLPATTTGRYLDLLETSFVLQRLPPFVRGRSQRLIKTP
                     KVYFADSGLAAYLADVAEIDARAGERLRGALYETYVAQNLRAIVDLHLGRAELAFWHV
                     QGRHEVDFVVATARAAVAIEIKAAARFDDADLAGLRAFA"
     gene            1452..2048
                     /locus_tag="VFA35_09810"
     CDS             1452..2048
                     /locus_tag="VFA35_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZT56511.1"
                     /translation="MDENTRSLVSRALQNDRDAVDVLFQRYRDRLRSGLRKLIGPKYR
                     ILMADSEDAAQDAILSALRRLHQFEYRGDGSFLAWLLKGAEFEVVRRIRALETQKRSA
                     KGGIVQLDTEAGHVVPDKDRSPSEALDEQELAERVRQALQQLPDREREVIVLRRYLEL
                     DTEEICAEMGLPTAGAVRALLSRAQARLAGLLSRDGEE"
     gene            2056..>2455
                     /locus_tag="VFA35_09815"
     CDS             2056..>2455
                     /locus_tag="VFA35_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56512.1"
                     /translation="MPKTEVGGGNAHGDTAGLVQRAVEEFIDRCGAGENVDPEQFARG
                     FAEGARGRIAEQCRNYLHFDDFVGRQAWSADELDAPSSRTFGDFLIQEELGRGGMGIV
                     YLAHQRSLNRRVALKVMASGLVLSKRHVERF"
BASE COUNT          365 a          894 c          867 g          329 t
ORIGIN      
        1 gcgaacgccc gcaggccggc gaggtcggcg tcgtcgaacc gcgccgccgc cttgatctcg
       61 atcgcgaccg ccgcgcgcgc ggtcgcgacc acgaagtcca cctcgtggcg gccttgcacg
      121 tgccagaacg cgagttcggc cctgcccagg tgcaggtcga cgatggcgcg caggttctgc
      181 gcgacatacg tctcgtacag cgccccgcgc aaccgctcgc cggcgcgcgc gtcgatctcg
      241 gccacgtccg cgaggtacgc cgcgagcccc gaatcggcga agtacacctt cggcgtcttg
      301 atcagccgct gcgacctgcc gcgcacgaac ggcggcaggc gttgcagcac gaacgacgtc
      361 tccagcaggt cgagatagcg gcccgtggtg gtcgccggca gcttggcgtc gcggccgatg
      421 ccgctctcgt tgccgagctg gcccgaccgc agggccgtga gccgcagcac gcgccggaac
      481 gccgccaggt cggcgacctg agcgaggtcg cgcacgtcgc gctcgaggta gctctgctcg
      541 tagccgcgca gccacagatc gcgatccagc gccgggtcca gcgccagctg cggcatgccg
      601 ccgcgcagga cctcctcgtc ggtgaccgcc gtgaactcgc ggcgccgcgg cagttcaccg
      661 gccgcgaaca gctgcgcgag ccagggctcg atctgcgtcg cacgcgcccg ctcgcggcgc
      721 gtcaacgggt gcatgggcag gtagaccgcg cggccggcca atgactcgcc caccgaacgc
      781 agcagccgaa agttcgccga gcccgacagc acgatctggc cgggccggcg gcggcggtcc
      841 acgatccgct tgaccgcgag cagcagctcg ggacagcgct gcgcctcgtc gaccgtcagc
      901 ggctcgtcgc cggcgacgaa cgcgtccggg tcgcggcgcg cctgttcgag caccggccag
      961 tcgtcgagcg tgacgtagcg gcggtcgcgc agccgcgcgt cgtgctgcag gaacgtggtc
     1021 ttgcccgact gccgcaggcc gctgatcacg accaccggca gcgcggccag tgcggcagcg
     1081 accttcagcc ccagctcgcg atccaggtag cttgtcatga ttcaccatcg atgatggcat
     1141 tatgacatca tgctggtcgg ctacttgcca cttgcgaccg gagaacccgg gcgccgtgga
     1201 aagcgttgcc cctgcgcacc catgcgtcgg cgcgacggcg cccgctgcct cggcgcgacc
     1261 ccgacgcgcc gtaaacaccc tcacggatcg aaaaaccgcg tgcgctcgtc agggtcctgg
     1321 ctctctgcag gagtaccatg ctggacgccg ccgctctcgc cgatccgttc cgccccgtgc
     1381 ccggcgccgt cccttgccgg acagccggac cgcggcagaa tcggtgcgcc catcgggcgg
     1441 tccgcttccc attggacgaa aacacccgca gtctcgtcag ccgcgccctg cagaacgatc
     1501 gggacgcggt cgacgtgctc ttccagcgct accgcgatcg cctgcggtca gggctgcgca
     1561 agctcatcgg cccgaagtac cggatcctga tggcggacag cgaggacgcg gcgcaggacg
     1621 cgatcctctc cgcgctgcgg cggctgcacc agttcgagta ccgcggcgac gggtcgttcc
     1681 tcgcgtggct gctgaagggc gccgagttcg aggtcgtgcg gcgcatccgc gcgctcgaga
     1741 cgcagaagcg cagcgcgaag ggcggcatcg tgcagctcga caccgaggcc ggccacgtgg
     1801 tgcccgacaa ggacaggtcg ccgtccgagg cgctcgacga gcaggagctc gccgaacgcg
     1861 tgcgccaggc gctgcagcag ctgcccgacc gcgagcgcga ggtgatcgtg ctgcgccgct
     1921 acctcgagct cgacaccgag gagatctgcg ccgagatggg cctgccgacg gcgggcgccg
     1981 tgcgcgcgct cttgtcgcgc gcgcaggcgc ggctcgcggg gctcttgtcg cgggacggcg
     2041 aggaatgaca tggccgtgcc gaagaccgaa gtcggcggag gcaacgcgca cggcgacacc
     2101 gcgggcctcg tgcagcgcgc ggtcgaggag ttcatcgacc gctgcggcgc cggcgagaac
     2161 gtcgatcccg agcagttcgc gcgcggcttc gccgagggcg cgcgcggcag gatcgccgag
     2221 cagtgccgca attacctgca cttcgacgac ttcgtcggcc gccaggcgtg gtcggcggac
     2281 gagctggatg cgccgagcag ccgcacgttc ggcgacttcc tgatccagga ggagctcggc
     2341 cgcggcggca tgggcatcgt ctacctcgcg caccagcggt cgctcaaccg ccgcgtcgcg
     2401 ctgaaggtga tggcgagcgg cctcgtgctc agcaagcgcc acgtcgagcg cttcc
//
LOCUS       DASRDG010000586         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_321340, whole genome shotgun sequence.
ACCESSION   DASRDG010000586 DASRDG010000000
VERSION     DASRDG010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321340"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFA35_09820"
     CDS             <1..405
                     /locus_tag="VFA35_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56513.1"
                     /translation="GWNEEFGNGCPGSAGVPDMRAQTEPTVGRTFIFQLANCAPATLL
                     RLVVVGLSNTVAGGSPLPAPLGSLGMPGCSLLVSINFNPSCGQDGYGVIRIPNAAMYD
                     GMGFFMQGFAIEPGVNPGGMISSNGLHCVVGM"
     gene            complement(551..1357)
                     /locus_tag="VFA35_09825"
     CDS             complement(551..1357)
                     /locus_tag="VFA35_09825"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZT56514.1"
                     /translation="MQALLEFLVRHGYAVVFVWVLVGQAGLPLPAIPVLLAAGALAGT
                     GKLSLAAVLAVSVVASVISDAVWFVIGRRAGSRVLALLCRVSLEPETCVRRTESTFAR
                     RGALTLVVAKFVPGLNTAAPPLAGTIGMSWRRFLLFNGAGALLWSVTFALPGFLFADQ
                     LEQVAAEAALTGAWLFGILVALVAAFLAAKIVRRWLFLRRLRIARIAPHELRALLDSG
                     DPPFVVDLRLAEDFAHEPETVPGAFHLTVEEIEAQHAAIPRDRDVVLYCT"
     gene            complement(1427..>3188)
                     /locus_tag="VFA35_09830"
     CDS             complement(1427..>3188)
                     /locus_tag="VFA35_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56515.1"
                     /translation="HNIIGILTESFGRPEPTPITQSLDRRLPYGDYPDPVPSQEWHAR
                     QTIDYLQTANFAILDYAARYREELLLGIWTMGDRAIRLGGEDHWTPTPKLVAAARARG
                     DDSPFRDPLLRDPRAYVLRSDQPDHGALVRFVRALQKSGVEVLMAQQPFEVAGVTAPA
                     GSFVVRCAQAFRAHVLDMFEPQWHPDDMKDGRPVPPYDAAGWTLAMQTGVDAIRVFGD
                     LDGPFAPLDALAECGTRAVPSGGAGWRLDPADSHAVTAVNRLLAARVAVERLGDGSFY
                     VPNGDGDAIAAAVRKAVAGLGVAAAKVDAQTDEVRPRLRQPRIGLFDVFGGSMPVGWD
                     QWTLLQFEFPVQRVWGDRIARGELGADFDALVFHAGLPGPRDLGRAGMQRDPETLQKL
                     AKALPPFEDWSDLAARATPLAADTAVPALRTFVERGGALIALGGECEKVIRHFELPIR
                     VGTFVPDPSAEGGERRTRREEFYVPGSLLALDIDLRHPLAAGMRQYQAAMFNANSPVF
                     EVTGPGPEVVARYRVWDTLVSGWALGDDHLAGKAAIVSVPLGKGRIVLFGADVTYRGQ
                     PVGTFKPLFSALLQSAMAGQ"
BASE COUNT          467 a         1189 c         1143 g          389 t
ORIGIN      
        1 ggctggaacg aagagttcgg caacggctgc cccggcagcg ccggcgtgcc cgacatgcgc
       61 gcgcagaccg agcccaccgt cggtcgcacg ttcatcttcc agctcgccaa ctgcgcgccg
      121 gcgacgctgc tgcgcctggt cgtggtcggc ctctccaaca cggtcgccgg cggctcgccg
      181 ttgccggcgc cgctcggctc cctcggcatg cccggctgca gcctcctcgt cagcatcaac
      241 ttcaatccgt cgtgcgggca ggacggctac ggcgtgatcc ggatccccaa cgccgcgatg
      301 tacgacggca tgggcttctt catgcagggc ttcgcgatcg agccgggcgt gaacccgggc
      361 ggcatgatca gctccaacgg gctccactgc gtggtcggca tgtaggtgcg catcagcccg
      421 cgcgcgacgc gacgaccacg gggaagccgg cgtcgcgcca gccgtcgagc ccaccgagca
      481 gcgggcgcac gcgcgtgatg ccgcgcctcg ccagcagcag cgccacacgg gcgctggtga
      541 cctcgttggg tcaagtacag tagagcacga cgtcgcggtc gcgcgggatc gcggcgtgct
      601 gcgcttcgat ctcctcgacc gtcaggtgga acgcgccggg cacggtctcc ggctcgtgtg
      661 cgaagtcctc ggcgaggcgc aggtcgacga cgaacggtgg gtcgccgcta tcgagcagcg
      721 cgcgcagctc gtgcggcgcg atccgcgcga tgcgcagccg gcgcaggaac agccaccggc
      781 gcacgatctt cgccgcgagg aacgccgcca ccagcgcgac caggatcccg aacagccacg
      841 cgccggtgag cgcggcctcg gccgcgacct gttcgagctg gtcggcgaac aggaagcccg
      901 gcagcgcgaa cgtgacgctc cacagcagcg cgcccgcgcc gttgaacagc aggaagcggc
      961 gccagctcat gccgatcgtg cccgcgagcg gcggcgccgc ggtgttcagg cccggcacga
     1021 acttggcgac caccagcgtc agcgcgccgc ggcgcgcgaa cgtcgactcg gtgcggcgca
     1081 cgcaggtctc cggttcgagc gacacgcggc acagcaatgc cagcacgcgg ctgccggcgc
     1141 ggcggccgat cacgaaccac accgcgtcgc tgatcaccga ggcgacgacg gagacggcga
     1201 gcaccgccgc gaggctcagt ttgcccgtgc cggccagcgc gcccgcggcc agcagcaccg
     1261 ggatcgcggg cagcggcagg ccggcctggc cgaccagcac ccagacgaac acgaccgcat
     1321 agccgtgtcg gacgaggaat tcgagcagcg cctgcatcgc cgtgtaaggc gccctgtttc
     1381 gcaccaaggg ccgcgcggcg caagcgccgc gtacccggcc cgctcgctac tggcccgcca
     1441 tcgccgactg cagcagcgcc gagaacagcg gcttgaacgt gccgaccggc tggccgcgat
     1501 aggtcacgtc ggcgccgaac agcacgatgc gccccttgcc gagcggcacc gacacgatcg
     1561 ccgccttgcc cgcgaggtga tcgtcgccga gcgcccaacc ggacacgagc gtgtcccaga
     1621 cgcggtagcg cgcgacgacc tcgggtcccg ggccggtgac ctcgaacacc ggcgagttgg
     1681 cgttgaacat cgccgcctgg tactggcgca tgcccgccgc gagcggatgc cgcaggtcga
     1741 tgtcgagtgc cagcagcgaa ccggggacgt agaactcttc gcgccgcgtg cggcgttcgc
     1801 cgccctcggc cgacggatcg ggcacgaacg tgccgacgcg gatcggcagc tcgaagtggc
     1861 ggatcacctt ctcgcactcg ccgccgagcg cgatcagcgc gccgccgcgc tcgacgaacg
     1921 tccgcagcgc gggcaccgcg gtgtcggccg cgagcggcgt cgcgcgcgcg gcgaggtcgc
     1981 tccagtcctc gaacggcggc agcgccttgg cgagcttctg cagcgtctcc gggtcgcgct
     2041 gcatccccgc gcgcccgagg tcgcgcggac ccggcaggcc ggcgtggaac accagcgcgt
     2101 cgaagtcggc gccgagctcg ccgcgcgcga tgcggtcgcc ccacacgcgc tgcacgggga
     2161 actcgaactg cagcagcgtc cactggtccc agcccaccgg catcgagccg ccgaacacgt
     2221 cgaacaggcc gatgcgcggc tgccgcagcc gcggccgcac ctcgtcggtc tgcgcgtcga
     2281 ccttcgccgc cgcgacgccg agacccgcga ccgccttgcg caccgccgcg gcgatcgcgt
     2341 cgccatcgcc gttgggcacg tagaacgacc cgtcgccgag ccgctcgacc gcgaccctgg
     2401 ccgcgagcag ccggttgacg gccgtcaccg cgtggctgtc ggcgggatcg agccgccagc
     2461 cggcgccgcc ggaaggcacc gcgcgcgtgc cacactcggc gagcgcgtcg agcggcgcga
     2521 acggcccgtc gaggtcgccg aacacgcgga tcgcgtcgac gcccgtctgc atcgcgagcg
     2581 tccacccggc ggcgtcgtac ggcggcacgg gcctgccgtc cttcatgtcg tcgggatgcc
     2641 actgcggctc gaacatgtcg agcacgtgcg cgcggaacgc ctgcgcgcag cggaccacga
     2701 acgagcccgc gggcgcggtg acgccggcga cctcgaacgg ctgctgggcc atgagcacct
     2761 cgacgccgct cttctgcagc gcgcgcacga accgcaccag cgcgccgtgg tcgggctggt
     2821 cgctgcgcag cacgtaagcg cgcggatcgc gcagcagcgg gtcgcggaac ggactgtcgt
     2881 cgccgcgggc gcgcgccgcc gcgaccagct tcggcgtcgg cgtccagtgg tcctcgccgc
     2941 ccagccggat cgcgcggtcg cccatcgtcc agatgccgag cagcagctcc tcgcggtagc
     3001 gcgcggcgta gtcgaggatc gcgaagttcg cggtctgcag gtagtcgatc gtctggcgcg
     3061 cgtgccattc ctggctcggc accggatcgg ggtagtcgcc gtacgggagc cggcggtcga
     3121 gcgactgggt gatcggtgtc ggctccggcc ggccgaacga ctcggtgagg atgccgatga
     3181 tgttgtgg
//
LOCUS       DASRDG010000587         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1242639, whole genome shotgun sequence.
ACCESSION   DASRDG010000587 DASRDG010000000
VERSION     DASRDG010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242639"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFA35_09835"
     CDS             <1..213
                     /locus_tag="VFA35_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HZT56516.1"
                     /translation="FAQGFDKINTTVTNVNAILVTISIAVVTIAIIWAGFKMIFQGAR
                     LSDVANVLIGGTLIGGATAFSTYIVS"
     gene            235..567
                     /locus_tag="VFA35_09840"
     CDS             235..567
                     /locus_tag="VFA35_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HZT56517.1"
                     /translation="MQAEAIYKGATRPAMKLGIPLVPLVLLFGTGMLLVLWVGSLVSW
                     WILPVVLAAFVPALAWMRFVTGKDDQRFRQMFVAAKLRFNDRNRRLWRSRSYAPYLYR
                     GTCDVWHR"
     gene            725..>2401
                     /locus_tag="VFA35_09845"
     CDS             725..>2401
                     /locus_tag="VFA35_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HZT56518.1"
                     /translation="MIARGGDYLRVWRLDGVAFECADEQVIAERHEAKCSLLRNLAGG
                     QFAVWEHRLHRTIADDLSLPPEPGFTRDLAEAHGRLLRRQRMMSNELYLTLVYRPTVS
                     TVGRAFQSTRRTRQDIARSQAEALRVMDEKSALVERVLRGFGPELLGSVTRNGREYSE
                     VAEFLGYLVNGCWRPVPMHAGPLYRTLPTARLSFGADKLEIRNGDQRRYAAFVDIHEY
                     ADAVEPGVLNALLYERSEFIETQSFSILPRREAMRALELQRDQLIASDDVVESQVTEM
                     DVALNELGDGQFCMGEYHYSLLVFGDDVADAGRRAAQAIGAVGEACSLQMRPVDLVAD
                     AAWFAQMPGNWQWRPREAKLSSRAFAALASGHNFARGKRDGNPWGEALVLLRTPSGQP
                     FYLNLHKSPEDEDSEDKKLPGNTLIIGSTGVGKTTLVMMLLALTRKWNPAPRLVLFDL
                     DRGSEIALRALGGRYFTLEAGTPTGCNPLQRDPTPARIQFWEQLVRTCIATPTLPLLP
                     ADERAIAAAVRSVAMMPRHLRRFSTVRQNLPKTGENSLYERLGRWCKGGAL"
BASE COUNT          373 a          849 c          811 g          368 t
ORIGIN      
        1 ttcgcccagg gcttcgacaa gatcaacacc accgtgacaa acgtcaacgc catcctggtg
       61 acgatctcga tcgcggtggt cacaatcgcg atcatctggg ccggcttcaa gatgatcttc
      121 cagggcgcgc ggctcagcga cgtcgccaac gtcctgatcg gcggcacctt gatcggcgga
      181 gccaccgcgt tctcgacgta catcgtcagc tgaccggcgt gcgaggaaga caccgtgcaa
      241 gccgaagcca tctacaaggg cgcgacgcgc ccggcgatga agctgggcat cccgctcgtg
      301 ccgctcgtcc tgctcttcgg caccgggatg ctcctggtcc tctgggtcgg cagcctggtg
      361 agttggtgga tcttgcccgt tgtcctcgcc gccttcgtcc cggcgctcgc ctggatgcgc
      421 ttcgtcaccg gcaaggacga ccagcgcttt cggcagatgt tcgtcgcggc gaagctgcgc
      481 ttcaacgacc gcaaccggcg cctctggcgc tcgcgcagct atgcgcccta cctctatcgg
      541 gggacatgcg atgtttggca tcggtagttc gaacacccgg acgggtgtgc cacccacggg
      601 tgtcccaccc aggggtgcac cgtggctgcg gagcgcccag tgcccccgcc actgggcccg
      661 cgcccgggcc gagaacccgc tgacgcgctt cgtccctttc tcgtcgctgg tctcccccgt
      721 cgatgtgatc gcccgagggg gcgactacct ccgggtgtgg cggctcgacg gcgtggcgtt
      781 cgagtgcgcc gacgagcagg tgatcgccga acgccacgaa gcgaagtgca gcctgctgcg
      841 caacctcgca ggcggccagt tcgcggtctg ggagcaccgg ctgcaccgga cgatcgccga
      901 cgacctgtcg ctgccgcccg agccgggatt cacccgcgac ctggccgagg ctcacggccg
      961 tctgctgcgc cggcagcgca tgatgagcaa cgagctctac ctgacactgg tctaccgtcc
     1021 caccgtctcg acggtcggcc gagccttcca gtcgacgcgc cgcacgcgcc aggacatcgc
     1081 ccgcagccaa gcggaggccc tgcgcgtgat ggacgagaag tcggcgctgg tcgaacgcgt
     1141 gctgcgaggg ttcgggccgg aactgctggg ctcggtcacg cgcaacggcc gcgagtactc
     1201 ggaagtggcc gagttcctcg gctacctggt caacggctgc tggcgcccgg tgccgatgca
     1261 cgccgggccg ctctatcgga cgctgccgac cgcgcgcctg tccttcggcg ccgacaagct
     1321 cgagatccgc aacggcgacc agcggcgcta cgccgccttc gtcgacatcc atgagtacgc
     1381 cgacgcggtc gagcccggcg tgctgaacgc gttgctctac gagcgcagcg agttcatcga
     1441 gacgcagagc ttctcgatcc tgccgcgccg cgaggcgatg cgcgcgctcg aactgcagcg
     1501 cgaccagctg atcgccagcg acgacgtcgt cgagagccag gtcaccgaga tggatgtcgc
     1561 gctgaacgaa ctcggcgacg gccagttctg catgggcgag taccactaca gcctgctcgt
     1621 gttcggcgac gacgtggccg atgccggacg gcgcgccgcc caggccatcg gtgcggtcgg
     1681 cgaagcctgc agcctgcaga tgcgcccggt cgatctggtc gccgacgccg cctggttcgc
     1741 gcagatgccg ggcaactggc agtggcggcc ccgcgaagcc aagctgtcgt cgcgtgcgtt
     1801 cgcggcgttg gccagcggcc acaacttcgc gcgcggcaag cgcgacggca acccatgggg
     1861 cgaggcgctg gtgctgctgc gcacgccaag cgggcagccg ttctatctga acctgcacaa
     1921 gagccccgag gacgaggact ccgaggacaa gaagctgccg ggcaacacgc tgatcatcgg
     1981 ctcgaccggc gtcggcaaga cgacgctcgt gatgatgctg ctggcgctga cgcgcaaatg
     2041 gaacccggcc ccgcgcctgg tgctgttcga cctggaccgc ggcagcgaga tcgcgctgcg
     2101 cgcgctcggc ggacgttact tcacgctcga agccggcacg ccgaccggct gcaacccgct
     2161 gcagcgcgac ccgacgccgg cccggatcca gttctgggag cagctcgtgc gcacctgcat
     2221 cgcgaccccg accttgccgc tgctgccagc cgacgagcgc gcgatcgccg cggcggtgcg
     2281 ctcggtcgcg atgatgccca ggcatctgcg gcgtttctcg acagtgcgcc agaacctgcc
     2341 gaagacgggc gagaacagcc tgtacgagcg gctcggccgc tggtgcaagg gcggcgcgct
     2401 c
//
LOCUS       DASRDG010000588         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_91523, whole genome shotgun sequence.
ACCESSION   DASRDG010000588 DASRDG010000000
VERSION     DASRDG010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91523"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VFA35_09850"
     CDS             complement(<1..1430)
                     /locus_tag="VFA35_09850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZT56519.1"
                     /translation="MTQDPDFGLHRDFYTFPPEALGEALPEFAIVREIGRGSMGIVYE
                     ATRKEDRQRVALKVLPPSPTLGERTLARFLREGRVMARVQHPHITRFLGQGHGEAGGV
                     RLHWFAMEFVDGVPLQERLQVGPLPLQNACEIAVQIARALQFAHERGVVHRDVKPGNV
                     ILRSQPTGGGTPSVAITDFGLARETGTGSMTESGAIVGTPMYMAPELVLGGSAQAGTL
                     GDVYALGASLYAMLTGRPPFEGPTAQSVLKQVVDEEPARPCRLRADLPADAEAVVLKA
                     MAKDPRQRYGSASELADDLERLLRGERTLARLPGPLALLARRAARRPLQAALLAAVLL
                     LSAGALQLFLSQRRQQVQEDLAEARNWLAQAATARDEQDRPRTPAQRRELLLAAVSAA
                     TDCLRSDADNVDARLLRARAHHELRQFHEAMLDLDAAERLGGLSSELLQRRIATLQQC
                     GDKASIHRLQQDLTRLLASDPSDRTRT"
     gene            complement(1427..>2101)
                     /gene="kdsA"
                     /locus_tag="VFA35_09855"
     CDS             complement(1427..>2101)
                     /gene="kdsA"
                     /locus_tag="VFA35_09855"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZT56520.1"
                     /translation="VALVFKSSFDKANRTSGQSFRGLGIDDGLAILAAVKSKVGLPVL
                     TDVHEAAQCAHAAEVVDCLQIPAFLCRQTDLLLAAAATGRAVNVKKGQFLAPEDVANV
                     LQKITAQNPNALITERGVSFGYRTLVVDFAGFPTMRSFGQPLVFDATHSVQRPGGEGR
                     STGGDRTKVPYLARAAVACGVDGLFLEVHEDPDHAPSDGPNMLRLGELPALLSELLRI
                     QDAVRR"
BASE COUNT          257 a          787 c          767 g          290 t
ORIGIN      
        1 gtgcgcgtgc ggtcgctcgg gtcgctcgcg agcaggcgcg tgaggtcctg ctgcagccgg
       61 tggatcgacg ccttgtcgcc gcactgctgc agcgtggcga tgcggcgctg cagcagttcg
      121 ctcgacagcc cgccgagccg ctcggcggcg tcgaggtcga gcatcgcctc gtggaactgg
      181 cgcagctcgt ggtgcgcccg cgcgcgcagc agccgcgcgt cgacgttgtc ggcgtcgctg
      241 cgcaggcagt cggtggcagc agagacggcc gcgagcagca gttcgcggcg ctgtgccggc
      301 gtgcgcggcc ggtcctgctc gtcgcgcgcg gtcgccgcct gcgccagcca gttgcgcgcc
      361 tcggcgaggt cctcctgcac ctgctggcgg cgctggctca agaacagctg cagcgcgccg
      421 gcggacagca gcagcacggc cgcgagcagc gccgcctgca gcggccgccg cgccgcgcgg
      481 cgcgcgagca gcgcgagcgg cccgggcagg cgcgccagcg tgcgttcgcc gcgcagcagc
      541 cgctcgaggt cgtccgcgag ctcggacgcc gacccgtagc gctggcgcgg gtccttcgcc
      601 atcgccttga gcacgaccgc ctcggcgtcc gccggcaggt cggcgcgcag gcggcacggc
      661 cgcgccggct cctcgtcgac cacctgcttg agcacgctct gcgcggtcgg gccctcgaac
      721 ggcggccggc ccgtcagcat cgcgtagagc gacgcgccga gcgcgtagac gtcgccgagc
      781 gtgcctgcct gggcgctgcc gccgagcacg agctccggcg ccatgtacat cggcgtgccg
      841 acgatcgcgc cgctctccgt catcgacccg gtgccggtct cgcgcgcgag cccgaagtcg
      901 gtgatcgcga cgctcggcgt gccgccgccg gtcggctggc tgcgcaggat cacgttgccc
      961 ggcttgacgt cgcggtgcac gacgccgcgc tcgtgcgcga actgcagcgc gcgcgcgatc
     1021 tgcacggcga tctcgcacgc gttctgcagc ggcagcggtc cgacctgcag ccgctcctgc
     1081 agcggcacgc cgtcgacgaa ctccatcgcg aaccagtgca ggcgcacgcc gccggcttcg
     1141 ccgtgcccct ggccgaggaa gcgcgtgatg tgcgggtgct gcacgcgcgc catcacgcga
     1201 ccctcgcgca ggaaccgcgc cagcgtgcgc tcgcccagcg tcgggctcgg cggcagcacc
     1261 ttgagcgcca cgcgctggcg gtcctccttg cgcgtcgcct cgtagacgat ccccatcgaa
     1321 ccgcggccga tctcgcgcac gatcgcgaac tccggcagcg cctcgcccag cgcctcgggc
     1381 gggaacgtgt agaagtcgcg gtgcagcccg aaatcgggat cctgcgtcat ctgcgcacgg
     1441 cgtcctggat gcgcagcagc tcgctgagca gcgccggcag ctcgccgagc ctcagcatgt
     1501 tcggcccgtc gctcggcgcg tggtcgggat cctcgtgcac ctcgaggaac aggccgtcga
     1561 cgccgcacgc gaccgccgcg cgcgcgaggt aggggacctt ggtgcggtcg ccgcccgtcg
     1621 acctgccctc gccgcccggc cgctgcaccg agtgcgtggc gtcgaacacc agcggctggc
     1681 cgaagctgcg catcgtcggg aagccggcga agtcgaccac cagcgtgcgg tagccgaagc
     1741 tgacgccgcg ctcggtgatc agcgcgttcg ggttctgcgc cgtgatcttc tgcagcacgt
     1801 tggcgacgtc ttcgggcgcc aggaactggc ccttcttgac gttgaccgcg cgcccggtcg
     1861 ccgcggccgc gagcagcagg tcggtctgcc ggcacaggaa cgccgggatc tgcaggcagt
     1921 cgacgacctc ggccgcgtgc gcgcattgcg cagcctcgtg cacgtcggtg agcaccggca
     1981 ggccgacctt gctcttgacg gcggcgagga tcgcgaggcc gtcgtcgatg ccgagcccgc
     2041 ggaagctctg ccccgaggtg cggttcgcct tgtcgaagct gctcttgaac accagcgcga
     2101 c
//
LOCUS       DASRDG010000589         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1602315, whole genome shotgun sequence.
ACCESSION   DASRDG010000589 DASRDG010000000
VERSION     DASRDG010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602315"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFA35_09860"
     CDS             <1..386
                     /locus_tag="VFA35_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HZT56521.1"
                     /translation="GMDNRTASFRVIAGSPKATRLETRCPGADVNPYLAMAAVIAAGL
                     HGVEQGLKLTQPPITGTNQGADDIPGAPRTLIETTRVFRQSDVARDWFGDDFVDHFAA
                     TREWEWRQWLDAVTDWELKRYFEII"
     gene            398..1537
                     /locus_tag="VFA35_09865"
     CDS             398..1537
                     /locus_tag="VFA35_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZT56522.1"
                     /translation="MAITRFSFPTTIHFGAGARALAGPHLRAQGLKRPLIITDKGLAA
                     LPLIAELSAGLAAAGLEVAVYGGVFGNPTGAQVMDGAAACRAHAADCVVGVGGGAALD
                     VAKVVGLLASHPGDVMEYVWDHPQVRAIDAATLPYFIALPTTSGTGSEVGRSSVISEE
                     DTHVKRVIFSPAILAKVVFADPELTLGLPPAVTAATGMDALTHNIESYLSPAYHPLCD
                     GIALEGARIGAHALLTAVREPGNLAARADMMMSSMMGAIAFQKDLGAVHSCAHALGTV
                     CDLHHGLANALMIEPVMAFNIEAVPHKFAELAHVVGAGSGDAFIPWLAQLKQQIGIAP
                     GLAAVGVKREHFGRLVEIATADGCHATNPRACVPADFERFFTEAF"
     gene            1539..>2051
                     /locus_tag="VFA35_09870"
     CDS             1539..>2051
                     /locus_tag="VFA35_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828739.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZT56523.1"
                     /translation="MARPLIIGISARIHHPIGPVLSLGGVYTKTLHYLEQSVAHWVLA
                     KEVLAVMIPAIESEGLIQRSEMSLSSYAEHLDALVLQGGADIAPESYGETPLTPEWSG
                     DRVRDRYEIDLFNAFVAAGKPVIGICRGCQLINVALGGTLFQDIPTQVDTAVAHRDVA
                     QYERALHDVNL"
BASE COUNT          330 a          693 c          708 g          320 t
ORIGIN      
        1 ggggcatgga caaccgcacc gcgagctttc gcgtgatagc aggcagcccg aaggcgacac
       61 ggctggagac gcgttgcccc ggggccgacg tgaacccgta cctggcgatg gccgcggtga
      121 tcgcggccgg gttgcacggt gtcgagcagg gcctgaaact gacgcagccg ccgatcaccg
      181 gcaccaacca gggcgcggac gacatcccgg gcgcgccgcg tacgctgatc gagacgacgc
      241 gggtgttcag gcaatccgac gtcgcccgcg actggttcgg cgacgatttc gtcgaccact
      301 tcgccgcgac ccgcgaatgg gagtggcggc agtggctcga cgccgtgacc gactgggaac
      361 tgaagcgcta cttcgagatc atctgaaacc tgttgccatg gccatcaccc gattttcctt
      421 tccgaccacg atccatttcg gcgccggtgc acgcgcgctc gccggcccgc atctgcgcgc
      481 gcagggcttg aagcgcccgc tgatcatcac cgacaaaggc ctggccgcgc tgccgctgat
      541 cgccgagctg agcgcggggc tggccgcggc gggcttggag gtcgcggtct acggcggtgt
      601 gttcggcaac ccgaccggcg cccaggtgat ggacggcgcc gcggcctgcc gcgcgcacgc
      661 ggccgactgc gtggtcggcg tcggcggtgg ggctgcgctc gatgtggcga aggtcgtcgg
      721 cctgctcgcg agccacccgg gcgacgtgat ggagtacgtg tgggaccacc cgcaggtgcg
      781 cgcgatcgac gccgcgaccc tgccgtactt catcgccttg ccgaccactt cgggcaccgg
      841 cagcgaggtc ggccgcagca gcgtgatcag cgaggaagac acgcacgtca agcgcgtgat
      901 cttctcgccc gcgatcctcg cgaaggtcgt gttcgccgac cccgaactga cgctgggtct
      961 gccgcccgcc gtgaccgccg cgaccggcat ggacgcgctg acccacaaca tcgagagcta
     1021 cctgtcgccc gcctaccacc cgctgtgcga cggcattgcg ctcgaaggtg cgcgcatcgg
     1081 cgcgcacgcg ttgctgacgg cggtgcgcga gccgggcaac ctggccgcgc gcgccgacat
     1141 gatgatgagc tcgatgatgg gtgcgatcgc gttccagaag gacctcggcg cggtgcactc
     1201 gtgcgcgcac gcgctgggca cggtgtgcga cctgcaccac ggcctggcga acgcgctgat
     1261 gatcgagccg gtgatggcat tcaacatcga ggcggtgccg cacaaatttg ccgaactggc
     1321 gcatgtcgtc ggcgcaggtt cgggcgacgc gttcatcccc tggctcgcgc agctgaagca
     1381 acagatcggc atcgcgcccg ggctggcggc ggtcggcgtg aagcgcgagc actttggccg
     1441 actggtcgag atcgcgacgg cagatggttg ccatgcgacc aatccgcgcg cctgtgtgcc
     1501 ggccgatttc gaacgcttct tcacggaggc attctgaaat ggcccgaccg ttgatcattg
     1561 gcatctcggc ccgcatccac cacccgatcg gcccggtgct cagcctgggt ggcgtctaca
     1621 ccaagacgct gcactacctc gagcaatcgg tcgcgcactg ggtgctcgcg aaagaagtgc
     1681 tcgcggtgat gatcccggcg atcgaaagcg agggcctgat ccagcgcagc gagatgagcc
     1741 tgagctcgta tgccgagcac ctcgacgctc tcgtgctgca gggtggcgcc gacatcgcgc
     1801 ccgagagcta cggcgagacg ccgctgacgc ccgaatggtc cggcgatcgc gtgcgcgatc
     1861 gctacgagat cgacctgttc aacgcgttcg tcgcggcggg caagccggtg atcgggatct
     1921 gccgcggctg ccaattgatc aacgtggcgc tcggcggcac cttgttccag gacatcccga
     1981 cgcaggtcga caccgcggtc gcgcaccgcg atgtcgccca gtacgagcgc gcattgcacg
     2041 acgtgaatct c
//
LOCUS       DASRDG010000590         4852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1243166, whole genome shotgun sequence.
ACCESSION   DASRDG010000590 DASRDG010000000
VERSION     DASRDG010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243166"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFA35_09875"
     CDS             complement(<1..519)
                     /locus_tag="VFA35_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZT56524.1"
                     /translation="MAPQSQPPSQPLRLHVPEPSGRPGQHTDFSYLHLSEAGAVRRPP
                     VDVAAADTGDLATALVRVLADDGGALGPWAPQLAPERLVQGLRAMLKTRIFDARMLIA
                     QRQKKISFYMQCLGEEAIACAHALALADGDMCFPTYRQQGLLLARDDIDMSEMMCQLM
                     SNERDPMKGRQLP"
     gene            650..856
                     /locus_tag="VFA35_09880"
     CDS             650..856
                     /locus_tag="VFA35_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HZT56525.1"
                     /translation="MSDHVYKLLELAGSSSTSIEDAVQNAIAKAHQSIRNIHWFTITE
                     TRGHVSDGKVAHWQVTLKVGFTLE"
     gene            859..1890
                     /locus_tag="VFA35_09885"
     CDS             859..1890
                     /locus_tag="VFA35_09885"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HZT56526.1"
                     /translation="MDAQRELAATVQALTAGAKGVLAADESGATIAKRFQAIGVESTE
                     AARRAWRSLLLGTAGLGEYISGVILFEETLGQRDAAGTPLPQLAARQGIVPGIKVDAG
                     KVALALAPGDEITQGLDGLAKRLEAYRQQGARFAKWRAVYNVGPQLPSRLALEANAEV
                     LARYAAICQAQGVVPIVEPEVLMDGAHTIERCAEVSEAAWREVFAALYRHRVVLELML
                     LKPSMVVPGKAHAAPAPPAEVARATLAVLRRSVPAAVAGIFFLSGGQTPAEASANLDA
                     LNRCGPQPWPLSFSYGRALQEPVLSAWRGQAANVALAQAALLKRARLNSAARAGRYDA
                     ALEAATDTA"
     gene            complement(1851..2267)
                     /locus_tag="VFA35_09890"
     CDS             complement(1851..2267)
                     /locus_tag="VFA35_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZT56527.1"
                     /translation="MARQLISSGSSFEAEIGYSRAVVDGDWVFVSGTTGFDYATMTIA
                     DDLVAQTEQCLKNIVAALAQAGCGIDDVVRVNYVLPPGNDFSACWPTLRRYFGTVRPA
                     ATMIHAGLADPRMRIEIEVTARRRQAVSVAASSAAS"
     gene            complement(2312..2938)
                     /locus_tag="VFA35_09895"
     CDS             complement(2312..2938)
                     /locus_tag="VFA35_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZT56528.1"
                     /translation="MDLSTWLTFFVAAWLISLSPGAGAVAAMSAGLSHGFMRGYVTSL
                     GLVLGIWTQIVVVGLGLGALIAASSLAFAAVKWLGVAYLVWLGIKQWRAPARPLVAAQ
                     ADAAGVSARTLILRGWMINAVNPKGTVFLLAVVPQFLDLSRALAPQYLVIAATLAFTD
                     LVVMGGYTALAARVLRLLKSPRQIKALNRTFGSLFIAAGALLALFKRA"
     gene            3004..4380
                     /gene="radA"
                     /locus_tag="VFA35_09900"
     CDS             3004..4380
                     /gene="radA"
                     /locus_tag="VFA35_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZT56529.1"
                     /translation="MAKDRSIYTCTECGGTSAKWLGQCPHCEAWNTLVESVAETAPKN
                     RYGAGTRGLIASQPVATLAHIEASDVDRQPTGIAELDRVLGGGIVAGGVVLIGGDPGI
                     GKSTLLLQALDSLSRTMKTLYVTGEESGAQVALRSRRLDLNASQVRVLAEIQLEKIAA
                     TLDQEQPAVCVIDSIQTVYSDQLSSAPGSVAQVRECAAQLTRIAKRSGTTIILVGHVT
                     KEGALAGPRVLEHIVDTVLYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGV
                     ANPSAIFLSTHGEPVAGACVLVTLEGTRPLLVEIQALVDSGGPSPRRLSVGIERDRLA
                     MMLAVLHRHAGVSAADQDVFVNAVGGVRISEPAADLAVMLAIQSSLRGKALPRGFIAF
                     GEVGLAGEVRPAPRGQERLREAAKLGFSVAVVPKANAPKGMTKAIEGLTIHAIERIEQ
                     AIDLVRGL"
     gene            complement(4387..>4852)
                     /locus_tag="VFA35_09905"
     CDS             complement(4387..>4852)
                     /locus_tag="VFA35_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56530.1"
                     /translation="TIGAALLSTLLLSTSAQAGLLGGGGAAGGLGGGLNAGVTPRSVS
                     IDGQAAGQASRDGALLPGRDAVKDGVGAAAQRGKEAGQSASAKAAESQSKTADGARDK
                     AAVAPASNAAKTGGGTAAATRRADASAEGSAQASRTDRSVSGGASAQGSVQR"
BASE COUNT          758 a         1721 c         1737 g          636 t
ORIGIN      
        1 cggcagctgg cggcccttca tcgggtcgcg ttcgttcgac atcagctggc acatcatttc
       61 cgacatgtcg atgtcgtcgc gcgccagcag caggccctgc tggcggtagg tcgggaagca
      121 catgtcgccg tccgcgagcg cgagcgcatg cgcacaggcg atcgcctcct cgcccaggca
      181 ctgcatgtag aacgagatct tcttctggcg ctgcgcgatc agcatgcggg cgtcgaagat
      241 gcgcgtcttc agcatcgcgc gcaggccctg caccagccgc tcgggtgcga gctgcggcgc
      301 ccagggcccg agcgcgccac cgtcgtcggc cagcacgcgc accagcgcgg tcgcgaggtc
      361 gccggtgtcg gccgccgcca cgtcgacggg cgggcgccgc accgcgccgg cctccgacaa
      421 atgcaggtac gaaaaatccg tgtgttggcc gggccgcccg ctgggctccg ggacatgcaa
      481 gcgaaggggt tgcgatggtg gttgggattg cggcgccatt cacgatcctc cgcgcccgcg
      541 ggcgcgtggg tcagggtagc gcttgcggcc ccgctgcgcc agcgggggat tgtggtcaca
      601 caccccagcg cctaagctca cagcacaaac ccacgcacgg agcacaacca tgagcgacca
      661 tgtctacaag ctgctggaac tcgcgggctc gtcgagcacg agcatcgagg acgcggtcca
      721 gaacgcgatc gccaaggcac accagagcat tcgcaacatc cactggttca cgatcaccga
      781 aacccgcggc cacgtcagcg acggcaaggt cgcgcactgg caggtgacgc tgaaggtcgg
      841 cttcacgctc gaatagcgat ggacgcgcag cgcgaactgg ccgcgacggt ccaggcgctg
      901 accgccggcg cgaagggcgt gctcgccgcc gacgagagcg gcgcgacgat cgccaagcgc
      961 ttccaggcga tcggcgtcga gtcgaccgaa gcggcgcgcc gcgcctggcg cagcctgctg
     1021 ctcggcaccg cgggcctggg cgagtacatc agcggcgtga tccttttcga ggagacgctg
     1081 ggccagcgcg acgcggccgg cacgccgctg ccgcagctcg ccgcgcgtca gggcatcgtg
     1141 ccggggatca aggtcgacgc cggcaaggtg gcgctcgcgc tcgcgcccgg cgacgagatc
     1201 acgcaagggc tggacggcct ggccaagcgc ctcgaagctt atcggcagca gggcgcgcgc
     1261 ttcgcgaagt ggcgcgccgt ctacaacgtc ggcccgcagc tgccgagccg gctcgcgctg
     1321 gaggccaacg ccgaggtgct ggcgcgctat gccgcgatct gccaggcgca gggcgtcgtg
     1381 ccgatcgtcg agcccgaggt gctgatggac ggcgcgcaca cgatcgagcg ctgcgccgag
     1441 gtcagcgagg cggcgtggcg cgaggtcttc gcggcgttgt accggcaccg cgtcgtgctc
     1501 gagctgatgc tgctgaagcc gagcatggtc gtgcccggca aggcgcacgc cgccccggcg
     1561 ccgccggccg aggtcgcgcg cgcgacgctc gcagtgctgc gccgcagcgt gccggcggcc
     1621 gtcgccggca tcttcttcct ctcgggcggg cagacgccgg ccgaggcgag cgcgaacctc
     1681 gatgcgctga accgatgcgg cccgcaaccg tggccgctga gcttctcgta cggccgggcg
     1741 ctgcaggagc cggtgctgag tgcctggcgc ggccaggccg cgaacgtcgc gctcgcgcag
     1801 gccgcgctgc tgaaacgcgc gcggctgaac agcgcggccc gcgccggtcg ctacgacgcc
     1861 gcgctggaag ccgccaccga caccgcctga cggcgtcgcg ccgtgacctc gatctcgatc
     1921 cgcatgcgtg ggtcggccag gccggcgtgg atcatcgtcg cggcgggacg gaccgtgccg
     1981 aagtacctgc gcagcgtcgg ccagcaggca ctgaaatcgt tgccgggcgg cagcacgtag
     2041 ttcacgcgca ccacatcgtc gatgccgcag cccgcctgcg cgagcgcggc cacgatgttc
     2101 ttcaggcact gctcggtctg cgcgaccagg tcgtcggcga tcgtcatcgt ggcgtagtcg
     2161 aagccggtcg tgccggagac gaacacccag tcgccgtcga cgacggcgcg cgagtagccg
     2221 atctcggcct cgaagctcga accggaactg atcagttggc gggccatgcg gcgctcccgg
     2281 ggtggcgaga gaggtcgtga gatcgcggcg atcaggcgcg cttgaacaac gccagcagcg
     2341 cgccggcggc aatgaacagg ctgccgaacg tgcggttcag cgccttgatc tggcgcggcg
     2401 acttcagcag ccgcagcacc cgggccgcga gcgcggtgta gccgcccatc acgaccaggt
     2461 cggtgaacgc gagcgtcgcc gcgatcacca ggtactgcgg tgcgagcgcg cgcgacaggt
     2521 cgaggaactg cggcacgacc gcgagcagga acaccgtgcc cttcgggttg accgcgttga
     2581 tcatccagcc gcgcaggatc agcgtgcgcg ccgacacgcc ggccgcatcg gcctgcgccg
     2641 cgacgagcgg acgcgccggt gcgcgccact gcttgatgcc gagccagacc aggtaggcca
     2701 cgccgagcca cttgacggcc gcgaacgcga ggctcgacgc tgcgatcagc gcaccgagcc
     2761 cgagcccgac gaccacgatc tgggtccaga tgccgagcac gagccccagg ctcgtcacgt
     2821 aaccgcgcat gaaaccatgg ctgagcccgg cgctcatcgc ggcgacggcg ccggcacccg
     2881 gcgacaggct gatcagccac gcagcgacaa agaaggtgag ccaggtcgag aggtccatcg
     2941 gccaaattat ccgcgctcac ggcgccgcct cgaagtcgcg gcgcttgccg cgacaatcaa
     3001 agcatggcga aagaccgatc gatctacacc tgcaccgagt gcggcggcac ctcggccaag
     3061 tggctgggcc agtgcccgca ctgcgaggcc tggaacacgc tggtcgagtc ggtcgccgag
     3121 acggcgccga agaaccgcta cggcgccggc acacgcgggc tgatcgcgag ccagccggtc
     3181 gcgacgctcg cgcacatcga agccagcgac gtcgaccgcc agcccaccgg catagccgag
     3241 ctcgaccgcg tgctcggcgg cggcatcgtc gccggcggcg tggtgctgat cggcggcgac
     3301 ccgggcatcg gcaagtcgac gctgctgctg caggcgctcg attccttgtc gcgcacgatg
     3361 aagacgctct acgtcaccgg cgaagagagc ggcgcgcagg tcgcgctgcg ctcgcgccgg
     3421 ctcgacctga acgcttcgca ggtgcgcgtg ctggccgaga tccagctcga gaagatcgcc
     3481 gccacgctcg accaggagca gcccgcggtc tgcgtgatcg actcgatcca gaccgtctac
     3541 agcgaccaac tgagttccgc gcccggctcg gtcgcgcagg tgcgcgagtg cgcggcacaa
     3601 ctcacgcgca tcgccaagcg cagcggcacg acgatcatcc tcgtcggcca cgtcacgaaa
     3661 gagggcgcgc tcgccgggcc gcgcgtgctc gaacacatcg tcgacacggt gctgtacttc
     3721 gagggcgaca cgcattcgag ctttcgcctg gtgcgcgcga tcaagaaccg cttcggcgcg
     3781 gtcaacgaga tcggcgtgtt cgcgatgacc gagaaggggc tgaagggcgt cgcgaacccg
     3841 agcgcgatct ttctctcgac gcatggcgag ccggtcgccg gcgcgtgcgt gctggtcacg
     3901 ctcgaaggca cgcggccgct gctggtcgag atccaggcgc tggtcgattc gggtgggccc
     3961 agcccgcggc ggctgagcgt gggcatcgag cgcgaccggc tcgcgatgat gctcgcggtg
     4021 ctgcaccgcc acgccggcgt cagcgccgcg gaccaggacg tgttcgtcaa cgccgtcggc
     4081 ggcgtgcgca tcagcgagcc ggccgccgac ctcgcggtga tgctggcgat ccagagcagc
     4141 ctgcgcggca aggcgctgcc gcgcggcttc atcgcgttcg gcgaggtcgg cctggccggc
     4201 gaggtgcggc cggcgccgcg cgggcaggag cgcttgcgcg aggcggcgaa gctcggcttc
     4261 tcggtcgccg tcgtgccgaa ggcgaacgcg ccgaagggca tgacgaaagc catcgaaggc
     4321 ctcacgatcc atgcgatcga acgcatcgag caggcgatcg acctggtgcg aggcttgtga
     4381 cgagactcag cgctgcacgc tgccttgcgc ggacgcgccg ccgctgaccg aacgatcggt
     4441 gcgcgaggct tgggccgaac cttcggccga tgcatccgca cgacgcgtcg cggcagcggt
     4501 cccgcccccg gtcttcgcgg cattcgaagc cggcgccacc gcggccttgt cgcgggctcc
     4561 gtcggccgtc ttggactggg actccgcggc cttggcggaa gccgactggc cggcttcctt
     4621 gccccgctgg gccgcggcgc ccacgccgtc cttcacggcg tcgcggcccg gcagcagcgc
     4681 cccgtcgcgg ctggcctgac ccgcggcttg gccgtcgatg ctcacgctgc gcggcgtgac
     4741 gccggcattc aggccaccac ccaatccgcc ggcagcgccc ccgccgccga gcaggccggc
     4801 ctgggccgag gtggacaaca gcagcgtcga cagcagcgcc gcgccgatcg tc
//
LOCUS       DASRDG010000591         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1697094, whole genome shotgun sequence.
ACCESSION   DASRDG010000591 DASRDG010000000
VERSION     DASRDG010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697094"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..955)
                     /locus_tag="VFA35_09910"
     CDS             complement(62..955)
                     /locus_tag="VFA35_09910"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZT56531.1"
                     /translation="MTSKQHEPSPSRVVAFLGLGVMGYPMAGHLARAGHRVTVYNRSP
                     ARAAQWVAEHGHASAATPRTAAANADIVFCCVGNDDDLRAVVLGEHGALAGMKPGAVF
                     VDHTTASAEVARELAAAARARDLQFIDAPVSGGNLGAVNGTLTVMCGGDAVAFDAIRP
                     VALAFAKAVTLLGASGAGQLAKMVNQIAIAGLIQGLAEAIAFGAKAGLDMAAVLDVIG
                     KGAAQSWQMDNRGPTMIDDRFDFGFAVDWMRKDLGIALDEARRNGARLPVTALVDQFY
                     ADIQAQGGNRLDTSSLVKRLR"
     gene            complement(995..1780)
                     /locus_tag="VFA35_09915"
     CDS             complement(995..1780)
                     /locus_tag="VFA35_09915"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZT56532.1"
                     /translation="MDLKTRLQWYGRELQSAGTWRLPIFKSKVHRARRSSTGSLAPLE
                     SRYCATPLYARGGTSDLSVFYQIFVEREYACLDDAIDVRLIIDCGANVGYSAAYLLNR
                     FPRARLIAIEPDNDNFRMLQMNLAPYRDRCTLIQAGVWSRSTGLVVSPVREGCGDEWG
                     ITVRECVPGEAPMVEAVDLGSLLEGSGEARISILKVDIEGSEREVFASNIDGWIDKFD
                     NLVIELHGEECEIAVRNALKDQDIAYSTSGELTVCKRVHRLAS"
     gene            complement(1833..>2807)
                     /locus_tag="VFA35_09920"
     CDS             complement(1833..>2807)
                     /locus_tag="VFA35_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZT56533.1"
                     /translation="KNCIITQREVGIDTDGWEQALKNTLRQAPDVILMGEIRDRETME
                     TFGTDGVVWCVDYAKANALLPQVLKDVAVTKRGLVIFVGATGSGKSTSLAAMVDFRNE
                     NSFGHIITIEDPVEFVHAHKNCIITQREVGIDTEGWEAALKNTLRQAPDVILMGEIRD
                     RETMEHAVAFAETGHLCLATLHANSANQALDRIINFFPEERRAQLLMDLSLNLKALIS
                     QRLMPRQEGRGRVAAVEIMLNTPLVSDLIFKGEVSEIKEIMKRSRELGMQTFDQALFD
                     LYEGHAITFEDALRNADSVNDLRLQIKLHSQRARSSDLSAGTEHLTIV"
BASE COUNT          438 a          963 c          913 g          493 t
ORIGIN      
        1 aactcgccga cctgcaaacc aagccggccc caagcaagcc ctgatctccg cgctgcgccg
       61 ttcagcgcag ccgcttcacc aggctcgagg tgtcgagccg gttgccgccc tgggcctgga
      121 tatcggcgta gaactggtcg accagcgccg tcactggcag gcgcgcgccg ttgcgacggg
      181 cttcgtcgag cgcaatgccg aggtccttgc gcatccagtc gaccgcgaag ccgaagtcga
      241 accgatcgtc gatcatcgtc gggccgcggt tgtccatctg ccagctctgc gcggccccct
      301 tgccgatcac gtcgagcacg gcggccatgt ccagcccggc cttcgcgccg aaggcaatcg
      361 cctccgccag gccttggatc agcccggcga tcgcgatctg gttgaccatc ttcgcgagtt
      421 ggccggcgcc gctcgcgccg agcagggtca cggccttcgc gaacgccagc gcgaccgggc
      481 ggatcgcgtc gaacgcgacc gcgtcgccgc cgcacatcac ggtcagcgtg ccattgaccg
      541 cgccgaggtt gccgcccgaa accggtgcgt cgatgaactg aaggtcgcgg gcccgcgccg
      601 ccgcggcgag ttcgcgcgcg acctcggccg acgcggtcgt gtggtcgacg aagaccgcgc
      661 cgggtttcat gcccgccaac gcgccgtgct cgccgagcac gaccgcgcgc aggtcgtcgt
      721 cgttgccgac gcagcagaac acgatgtcgg cgttcgccgc cgccgtacgc ggcgtcgcgg
      781 cactcgcatg gccgtgctcg gcaacccact gtgcggcccg ggccggcgag cggttgtaga
      841 ccgtcacccg atgccccgcg cgggcgaggt ggccggccat cggatagccc atgacgccga
      901 ggccgagaaa cgcgacgacg cgcgaaggcg agggttcgtg ctgcttggag gtcagcgggg
      961 caggcacttt ggagctgatg ttcacgcgtc ggcgttagga cgcgaggcga tgcacgcgct
     1021 tgcacaccgt cagttcgccc gaggtggagt acgcgatgtc ctggtctttc aaggcgttgc
     1081 gaaccgcgat ttcgcactct tcaccgtgca actcgatcac caggttgtcg aacttgtcga
     1141 tccagccgtc gatgttcgac gcgaacacct cccgctccga tccctcgatg tcgaccttga
     1201 gtatcgagat gcgcgcctcg ccggaacctt cgagcagcga accaaggtcg accgcctcaa
     1261 ccatgggcgc ctcgcccggc acgcactcgc gcaccgtgat gccccattcg tcaccgcaac
     1321 cttcacgaac cggcgacacc accagcccgg tcgagcgcga ccacacgccg gcctggatga
     1381 gcgtgcaccg gtcgcgatac ggtgcgaggt tcatctgaag catccggaag ttgtcgttgt
     1441 cgggctcgat cgcaatcaat cgcgcgcgcg ggaaccggtt gagcaaatag gccgcggaat
     1501 accccacgtt ggcgccgcag tcgatgatga gcctgacatc gatcgcgtcg tcgaggcagg
     1561 cgtactcgcg ctcgacgaag atctggtaga agacgctcaa gtcgctggtg ccaccacgcg
     1621 catagagcgg cgttgcgcag tagcgactct ccagcggcgc caggctgcct gtcgacgacc
     1681 ggcgtgcacg atgaaccttg gacttgaaaa tcggcaggcg ccaagtgcct gcgctctgaa
     1741 gttcgcgccc gtaccactgc agcctcgtct tcaggtccat tccctaccct gtcttcgttt
     1801 cggttccccc gcagcgcgca cggcaggatc ggctacacaa tcgtcaggtg ctcggtcccg
     1861 gccgacaggt cgctgctgcg cgcgcgctgg ctgtgcagct tgatctgcag ccgcaggtcg
     1921 ttgaccgagt cggcgttgcg cagcgcgtct tcgaaggtga tggcgtggcc ttcgtagagg
     1981 tcgaacagcg cctggtcgaa ggtctgcatc ccgagttcgc gcgagcgctt catgatctcc
     2041 ttgatctccg agacctcgcc cttgaagatc aggtccgaga ccagcggcgt gttcagcatg
     2101 atctcgaccg cggcgacccg gccgcgccct tcctggcgcg gcatcaggcg ctgcgagatc
     2161 agcgccttga ggttcagcga caggtccatc agcagctggg cgcgccgctc ttcggggaag
     2221 aagttgatga tgcggtcgag cgcctggttg gcactgttgg cgtgcagcgt cgccaggcag
     2281 aggtggccgg tctcggcgaa cgcgaccgcg tgctccatcg tctcgcggtc gcggatctcg
     2341 cccatcagga tcacgtcggg cgcctgccgc agcgtgttct tcagcgccgc ttcccagccc
     2401 tcggtgtcga tgccgacctc gcgctgcgtg atgatgcagt tcttgtgcgc gtgcacgaac
     2461 tcgaccgggt cttcgatcgt gatgatgtgg ccgaacgagt tctcgttgcg gaagtcgacc
     2521 atcgccgcga gcgacgtgct cttgcccgag ccggtcgcgc cgacgaagat cacgaggccg
     2581 cgcttggtga cggccacgtc cttcagcacc tgcggcagca gcgcgttggc cttggcgtag
     2641 tcgacgcacc agacgacccc gtccgtgccg aaggtttcca tcgtctcgcg gtcgcggatt
     2701 tcgcccatca ggatcacgtc gggcgcctga cgcagcgtgt tcttcagcgc ctgctcccag
     2761 ccgtcggtgt cgatgccgac ctcgcgctgc gtgatgatgc agttctt
//
LOCUS       DASRDG010000592         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1697221, whole genome shotgun sequence.
ACCESSION   DASRDG010000592 DASRDG010000000
VERSION     DASRDG010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697221"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VFA35_09925"
     CDS             complement(<1..914)
                     /locus_tag="VFA35_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HZT56534.1"
                     /translation="MATVALAGSDRRFECAAGDTLLRAGLRAGIGLSYECNAGSCGTC
                     KIDLVSGELDDLYPEAPGIKQRDRDRGRRLACQSVPRGDCTIKSRVADSYLTPNPPRR
                     RTVRLAGTRDITHDIREFSFRADGPAAFLAGQYAMLVLPGAPAPRAYSMSNTANGDGL
                     WKFMIRRTPTGQVSRSLFELAPGSEVELDGPFGLAYLREDSPRDIVCIAGGSGIAPMA
                     AILDAAAEHEHVRHLGAWLFYGGRGPADVPAIADLLATHHLERGLEWHPVVSVPALAE
                     GTDWSGEFGFVHELLPKKLPRPLPEYEF"
     gene            complement(925..1791)
                     /locus_tag="VFA35_09930"
     CDS             complement(925..1791)
                     /locus_tag="VFA35_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT56535.1"
                     /translation="MATPHTSLWADLQGVAFEQAYVDVDGIRTRYLHAGQKGKPALIF
                     IHGTGGHAEAYSRNLEAHGEHFDVYAIDMLGHGFTAKPDKPYLIDDYVAHLRGFMDKL
                     KIDRASLSGESLGGWVAAHFAVVHPERVDKLVLNTASGDKVNPEALARLRTLSVEAIN
                     DPSYERIKGRLEWLMHDKSKVNDDLVASRQAIYAQPSMKQAIHHILALHTPEARARYA
                     IKAEQWKALKAPTLVLWTDNDPTATVQVGQELADAIPGSQFVVMKGCGHWPQFEDAET
                     FNRIHIDFLQKG"
     gene            complement(1813..>2386)
                     /locus_tag="VFA35_09935"
     CDS             complement(1813..>2386)
                     /locus_tag="VFA35_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZT56536.1"
                     /translation="DFYATMGPSSAPPYRTSVAHILRLGHLVIKSTDFDATVSYFTDV
                     LNFAVSDSIDKTVTFLRCWPSPYHHSLGIGQGRKGSGLHHVALMVDSADDIGRSYWRL
                     QREHVPVVHGPGRHLPSGSMFLYYLDPDGLTIEYTFGMEEFPEVEPRAPRLLPPVPES
                     NDIWASKLDPRKASVGAIEQVPLTTTVSSH"
BASE COUNT          345 a          766 c          893 g          382 t
ORIGIN      
        1 tagaactcgt actccggcag cggccgcggc agcttcttcg gcagcagctc gtggacgaag
       61 ccgaactcgc cgctccagtc ggtgccctcg gccagcgccg gcaccgagac caccgggtgc
      121 cactcgaggc cgcgctcgag gtgatgcgtc gccagcaggt cggcgatcgc cggcacgtcg
      181 gccgggccgc gcccgccgta gaacagccac gcaccgagat ggcgcacgtg ctcgtgctcc
      241 gcggccgcgt cgaggatggc ggccatcggc gcgattcccg agccgccggc gatgcagacg
      301 atgtcgcgcg ggctgtcctc gcgcaggtag gcgaggccga acggcccgtc gagctcgact
      361 tcgctgccgg gcgcgagctc gaacagcgag cggctgacct ggccggtcgg ggtgcgccgg
      421 atcatgaatt tccaaagccc gtcgccgttg gcggtgttcg acatcgagta ggcccgcggc
      481 gccggcgcgc cgggcaggac gagcatcgcg tactggccgg cgaggaaagc ggcggggccg
      541 tcggcgcgaa agctgaactc gcgaatgtcg tgggtgatgt cgcgcgtgcc ggccagccgc
      601 accgtgcgtc gacgcggcgg gttcggcgtc aggtagctgt cggcgacgcg cgacttgatc
      661 gtgcagtcgc cgcgcggcac gctctggcag gcaaggcgcc ggccgcgatc gcgatcgcgc
      721 tgcttgatgc cgggtgcctc ggggtagaga tcgtcgagct cgccgctcac gaggtcgatc
      781 ttgcaggtgc cgcaggagcc ggcgttgcac tcgtaggaga ggccgatgcc ggcgcgcagc
      841 ccggcgcgca gcagcgtgtc gcccgcagcg cattcgaagc gtcggtcgct gccggcgagc
      901 gcgacggtgg ccatggccgc gccctcagcc cttctgcagg aagtcgatgt ggatgcggtt
      961 gaaggtctcc gcgtcctcga actgcggcca gtggccgcag cctttcatca ccacgaactg
     1021 cgagcccggg atggcgtcgg cgagctcctg gccgacctgg accgtcgcgg tcgggtcgtt
     1081 gtcggtccag agcaccagcg tcggcgcctt cagcgccttc cattgctccg ccttgatcgc
     1141 gtacctggcg cgcgcctccg gcgtgtgcag ggcgaggatg tgatggatcg cctgcttcat
     1201 ggacggctgg gcgtaaatcg cctggcggct ggcgacgagg tcgtcgttga ccttggactt
     1261 gtcgtgcatc agccattcga gccggccctt gatgcgctcg tacgacgggt cgttgatcgc
     1321 ctcgaccgag agcgtgcgca gccgagccag cgcctcggga ttcaccttgt cgccgctggc
     1381 ggtgttgagc acgagcttgt ccacgcgctc cggatggacg acggcgaagt gcgccgccac
     1441 ccagccgccg agcgattcgc cggaaaggga cgcccggtcg atcttcagct tgtccatgaa
     1501 gccgcgcaga tgggcgacgt agtcgtcgat caggtagggc ttgtcgggct tggcggtgaa
     1561 gccgtggccg agcatgtcga tcgcgtagac gtcgaagtgc tcgccgtgcg cctcgaggtt
     1621 gcgcgagtag gcctcggcgt ggccgccggt gccgtggatg aagatcagcg ccggcttgcc
     1681 cttctggccg gcgtgcaggt agcgggtgcg gatgccgtcg acgtcgacgt aggcctgctc
     1741 gaaggccacg ccttgcaggt cggcccagag gctggtgtgg ggggttgcca tggcggagct
     1801 cgcggtgagg gatcagtggg aggacacggt cgtcgtcagc ggcacctgct cgatcgcgcc
     1861 gaccgaggcc ttgcgcggat cgagcttcga ggcccagatg tcgttcgatt cgggcaccgg
     1921 cggcagcagc cgcggcgccc gcggctcgac ctccgggaac tcctccatgc cgaaggtgta
     1981 ctcgatggtc aggccgtccg ggtcgaggta gtagaggaac atgctgcccg acggcaggtg
     2041 ccggcccggg ccgtgcacca ccggaacatg ttcgcgctgc agccgccagt aggagcggcc
     2101 gatgtcgtcg gcagaatcga ccatcagcgc gacatggtgc aggccgctgc cctttcgccc
     2161 ctggccgatg ccgagcgagt ggtggtacgg gctcggccag cagcgcagga aggtgacggt
     2221 cttgtcgatc gagtcggaga cggcgaaatt gagcacgtcg gtgaagtagc tcaccgtcgc
     2281 atcgaagtcg gtcgacttga tcaccaggtg gccgaggcgc aggatgtgcg cgaccgaggt
     2341 ccggtagggc ggcgcgctcg agggccccat ggtcgcgtag aagtcg
//
LOCUS       DASRDG010000593         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_895612, whole genome shotgun sequence.
ACCESSION   DASRDG010000593 DASRDG010000000
VERSION     DASRDG010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895612"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFA35_09940"
     CDS             <1..209
                     /locus_tag="VFA35_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HZT56537.1"
                     /translation="RVGRTRVHLDRVEGLGDFLELEVVLRDDESAADGVAEAHALLRR
                     LRVDAAQLVSGAYVDLLRGGAAPG"
     gene            complement(216..1268)
                     /locus_tag="VFA35_09945"
     CDS             complement(216..1268)
                     /locus_tag="VFA35_09945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZT56538.1"
                     /translation="MKIVIVTDAWLPQVNGVVTTLLELLGGLEARGHEVRLIEPSGFR
                     RFACPGYAEIELAWRPGAEVARRIDAADADAVHIATEGPLGAAARRHCLRRGMAFTTA
                     FHTRFPDILAKALHVPSSWGYAWFRRFHAPSSGVMVPTAGMLEILKQHRFERLLRWSH
                     GVDLRLFRPVEPAVLDLPRPVFLYVGRVSYEKNLEAFLKLELPGSKLVYGVGPLLPAL
                     REKYPQVHWRGVVPREALPAVYSAADAFVFPSHSETFGLVMLEAMACGTPVAAFPVTG
                     PLDVVGDSDGGVLDADLARAAMRALDVPRERARARALEFDWERVCDQFVSFLVPADGR
                     PGQAARPARPTVGANA"
     gene            complement(1292..2152)
                     /locus_tag="VFA35_09950"
     CDS             complement(1292..2152)
                     /locus_tag="VFA35_09950"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZT56539.1"
                     /translation="MQRDFIPATEPPPGLRCAEDDDPPAGLHVRTVWISDLHLGTPGC
                     QAGALLAFLKHVECETLYLVGDIIDGWQLRRAWYWPQAHNDVVQKLLRKARKGTRVIF
                     VPGNHDEFARKYVQHNFGGVEVVDEWVHETADGRRLWVIHGDLFDGVIQCAKWLAHLG
                     DTMYEFTLKLNRHLNSLRARLGLPYWSLSKYLKLKVKRAVSYVSDFEVAVAREARKRG
                     LQGVVCGHIHHAELREIDGVLYANDGDWVESLTALIEHGDGRLEILDWAGHAARAHDE
                     ALAPALAPAV"
BASE COUNT          343 a          806 c          804 g          341 t
ORIGIN      
        1 tgcgcgtcgg ccgcacgcgc gtgcacctcg atcgggtcga aggtctgggc gactttctcg
       61 aactcgaggt ggtactgcgc gacgacgaga gcgccgccga cggcgtggcc gaagcccacg
      121 cgctgctgcg ccgcctgcgc gtcgatgcgg cgcaactcgt ctcgggcgcg tatgtggatc
      181 tgctgcgtgg tggcgcagcg cctggctgac gccggctacg cgttcgcgcc gaccgtcggt
      241 cgtgcgggcc gcgcggcctg acccggccgg ccgtcggccg gcaccaggaa cgacacgaac
      301 tggtcgcaca cgcgctccca gtcgaattcg agcgcacgtg cacgggcacg ctcgcgcggt
      361 acgtcgagcg cgcgcatcgc ggcgcgcgcg aggtcggcgt cgagcacgcc gccgtcggag
      421 tcgccgacga catcgagcgg cccggtcacc gggaaggccg cgaccggcgt gccgcaggcc
      481 atcgcctcca gcatcaccag gccgaaggtc tcgctgtggc tggggaacac gaacgcatcg
      541 gcggcgctgt agaccgccgg cagcgcctcg cgcggcacga cgccgcgcca atgcacctgc
      601 gggtacttct cgcgcagcgc cggcagcagc gggccgacgc cgtagaccag cttcgagccg
      661 ggcagctcga gcttcaggaa ggcttcgagg ttcttctcgt acgagacgcg gccgacatac
      721 aggaacaccg gccgcggcag atcgagcacg gccggctcga ccggcctgaa cagccgcagg
      781 tcgaccccgt gcgaccagcg cagcaggcgc tcgaaacgat gctgcttcag gatctcgagc
      841 atgcctgcgg tcggcaccat cacgcccgac gacggcgcat ggaagcggcg gaaccacgcg
      901 tagccccagg acgacggcac atgcagcgcc ttcgcgagga tgtcgggaaa gcgggtgtgg
      961 aacgcggtcg tgaacgccat gccgcggcgc aggcagtggc ggcgcgcggc cgcgccgagc
     1021 gggccttcgg tggcgatgtg gaccgcgtcg gcatcggccg catcgatgcg acgcgcgacc
     1081 tcggcgcccg gccgccacgc gagttcgatc tcggcgtagc cggggcaggc gaagcgcctg
     1141 aagcccgagg gctcgatcag ccgcacctca tggccgcgcg cttccagccc acccaaaagt
     1201 tcgagcaagg tggtgacgac gccgttgacc tgcggcagcc atgcgtcggt gacgatcacg
     1261 atcttcattt gcatctccgt gcagtgtggc ttcaaaccgc cggcgcgagt gcaggcgcga
     1321 gcgcttcgtc gtgcgctcgt gcggcgtgcc ccgcccagtc gagtatttcc agccgcccgt
     1381 cgccatgctc gatcagcgcg gtcaggctct cgacccagtc gccgtcgttc gcgtacagca
     1441 cgccgtcgat ctcgcgcagt tcggcatggt ggatgtggcc gcacacgacg ccctgcagcc
     1501 cgcgcttgcg cgcctcgcgc gcgaccgcga cctcgaagtc gctgacgtag ctgaccgcgc
     1561 gcttgacctt cagcttcagg tacttcgaca ggctccagta cggcagcccc aggcgcgcgc
     1621 gcagcgagtt caggtgtcgg ttcagcttca gcgtgaactc gtacatcgtg tcgcccaggt
     1681 gtgcgagcca cttggcgcac tggatcacgc cgtcgaacag gtcgccgtgg atgacccaga
     1741 gccggcggcc atccgccgtc tcgtggaccc actcgtcgac gacctcgacg ccgccgaagt
     1801 tgtgctgcac gtacttgcgc gcgaactcgt cgtggttgcc cggcacgaaa atcacgcgcg
     1861 tgcccttgcg cgccttgcgc agcagcttct gcaccacgtc gttgtgcgcc tgcggccagt
     1921 accaggcgcg ccgcagctgc cagccgtcga tgatgtcgcc gacgaggtag agcgtctcgc
     1981 actcgacgtg cttcaggaac gcgagcagcg cgccggcctg gcagcccggc gtgcccaggt
     2041 gcaggtcgga gatccagacc gtgcgcacgt gcaggccggc gggcggatcg tcgtcttcgg
     2101 cgcaccgcag gccgggcggc ggttccgtcg cgggaatgaa gtcgcgctgc atgaggcggg
     2161 atcgtcgcgg cggcccgtga cggccgcatg tcggctgcgt gacgtgtcga tgacgggcgc
     2221 ttagcggtac acggccatgt catgaaactg ccacagcgcg ttgctagcct tgccggcttt
     2281 gctgcgtgct tccc
//
LOCUS       DASRDG010000594         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_452376, whole genome shotgun sequence.
ACCESSION   DASRDG010000594 DASRDG010000000
VERSION     DASRDG010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452376"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..601
                     /locus_tag="VFA35_09955"
     CDS             131..601
                     /locus_tag="VFA35_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZT56540.1"
                     /translation="MRHWLMKSEPSECSIDDLARAPAQTVAWVGVRNYQARNFMRDEM
                     RVGDGVLFYHSSCPEPGIAGLAEVASAAYADATQFDPTSHYFDAKSRPEAPRWLHVDV
                     KLVRKTPLLPLAAMRAAPTLATMRVLERGNRLSITPVTGAEWRAVLALLGKAAQ"
     gene            complement(623..1672)
                     /locus_tag="VFA35_09960"
     CDS             complement(623..1672)
                     /locus_tag="VFA35_09960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZT56541.1"
                     /translation="MNETTAALSQERIETTRSLARALDADAAGLAAVLLEMLDRVDVL
                     LSIKDAATGRYLHVNPAMAALFGRAPAAMLNATDADLLEPAQAAAMRAAEQTALAHDA
                     PMLSEHKIERDGQRREFSVTRMALPGANGSPAHQVLSVWVDRTAAQQREQQLQQALAQ
                     LEQQQLAAETLRRETQDQSLRDGTTGLYQRVHFDDQLRREVDLSSREHREFALVSIAL
                     DPLNDAVKSAGDDARTRVLEALGRLLRSNTRAMDASCRLDEDRFAVLLSGVGLATAHS
                     RMEGLRRQCATQIVVLEGRDLGFTVSMGVASFPHTAHSEPDLMQAADTALREAQKRGG
                     NHVALASIRFELGAA"
     gene            complement(1839..>2457)
                     /locus_tag="VFA35_09965"
     CDS             complement(1839..>2457)
                     /locus_tag="VFA35_09965"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HZT56542.1"
                     /translation="AGGALLAESVRHVEANLASLEKNLSKATHGDLLDAVRAPWPALK
                     AALLVPGEAAALAEVDAQAERMLLQADRLVSALEAGAMLASLHVINVSGRQRMLSQRV
                     AKQALLAAQLSGSASVQAREQGEQARAAFEDAMKYLRSVPLSTPDIVDALQAAGRAWT
                     ELLASLGQARGAAGQRALLDASEALLAAFEVLTERYERSMQMLMG"
BASE COUNT          327 a          887 c          866 g          377 t
ORIGIN      
        1 gttggtgtga tcgtctcgcg tcagatgaac ccgaagccgg gctgacctcg cccacctgaa
       61 ctcctggttc aggaatgcgg ctcacggttc gcggcggaca cctttcatcc gcgagctgcg
      121 agcctgcccc atgcgccact ggttgatgaa aagcgagccg tccgagtgct cgatcgacga
      181 cctggcgcga gcgccggcac agaccgtcgc gtgggttggg gtgcgcaact accaggcgcg
      241 caatttcatg cgcgacgaga tgcgcgtcgg cgacggcgtg ctgttctatc actcgtcgtg
      301 ccccgagccg ggcattgccg gcctggccga ggtggcgagc gccgcgtacg cggacgcaac
      361 ccagttcgat ccgacgagcc actacttcga tgccaagtcc aggcccgagg cgccgcgctg
      421 gctgcatgtc gacgtgaagc tggtgcgcaa gacgccgctg ctgccgctcg ccgcgatgcg
      481 cgccgcgccg acgctggcga cgatgcgcgt gctcgagcgc ggcaaccggc tgtcgatcac
      541 gccggtcacc ggcgccgagt ggcgcgcggt gctggccttg ctcgggaagg cagcccagta
      601 gcgttcggtg tgacccgcag cctcaggccg cgccgagctc gaagcggatg ctcgcgagcg
      661 cgacgtggtt gccgccgcgc ttttgcgcct cgcgcagcgc ggtgtcggcg gcctgcatca
      721 gatcgggctc gctgtgcgcg gtgtgcggaa agctcgccac gcccatcgac acggtgaacc
      781 cgaggtcgcg cccctcgagc acgacgatct gggtcgcgca ctggcgccgc agcccttcca
      841 tgcgcgagtg cgcggtcgcg aggccgacgc ccgagagcag caccgcgaag cggtcttcgt
      901 cgaggcggca cgaggcgtcc atcgcgcgcg tgttgctgcg cagcaggcgg ccgagcgctt
      961 cgagcacgcg ggtgcgggcg tcgtcgccgg ccgacttgac cgcgtcgttg agcggatcga
     1021 gcgcgatcga gacgagtgcg aactcgcggt gttcgcgcga cgacagatcg acctcgcgcc
     1081 gaagctggtc gtcgaaatgc acgcgctgat acaggccggt ggtgccgtcg cgcaggctct
     1141 ggtcctgcgt ctcgcggcgc agcgtttcgg cggcgagctg ctgctgctcg agctgcgcga
     1201 gcgcctgctg cagctgctgc tcgcgttgct gcgcggcggt gcgatcgacc cacacgctga
     1261 gcacctggtg cgccgggctg ccgttggcgc ccggcagcgc catgcgcgtg acgctgaatt
     1321 cgcgccgctg cccgtcgcgt tcgatcttgt gctcgctcaa catcggcgcg tcgtgcgcca
     1381 gggcggtctg ctcggcggcg cgcatggccg cggcctgggc gggctcgagc aggtcggcat
     1441 cggtcgcatt cagcatcgcg gcgggcgcgc gcccgaacag cgccgccatc gccgggttca
     1501 cgtgcaggta gcggccggtc gcggcgtcct tgatcgagag cagcacgtcg acccggtcca
     1561 gcatctccag cagcaccgcg gccagcccgg ccgcgtcggc gtcgagcgca cgcgccagcg
     1621 agcgcgtcgt ctcgatgcgc tcctgcgaca gagccgctgt cgtctcgttc atatctctgc
     1681 gcgctcctcg gcggcccgcc gtcgtcgaca gccgggtccg cctcattcga ttcatcttgg
     1741 cccgcgctcg gcactctggc aagcgcgacc tctgtcaatc ggagatcgtc tccctagatc
     1801 gatgatagcc gcggcgcgga cggacgcacg ggcggcgatc agcccatcag catctgcatg
     1861 ctgcgctcgt agcgctcggt cagcacttcg aaggcggcga gcagcgcctc gctcgcgtcg
     1921 agcagcgccc gctggcccgc ggcgccacgg gcctggccga gcgacgcgag caactcggtc
     1981 caggcgcggc cggcggcctg cagcgcgtcg acgatgtcgg gcgtgctcag gggcacgctg
     2041 cgcaggtact tcatcgcatc ctcgaacgcg gcgcgcgcct gctcgccctg ctcccgcgcc
     2101 tgcaccgagg cgcttcccga caactgcgcc gcgagcagcg cctgcttggc gacgcgctgc
     2161 gacagcatgc gctggcggcc cgacacgttg atcacgtgca ggctcgcgag catcgcaccg
     2221 gcctcgagtg ccgagacgag gcggtcggcc tgcagcagca tgcgttcggc ctgcgcgtcg
     2281 acctccgcca gcgccgccgc ttcgcccgga accagcagcg cggccttcag cgccggccag
     2341 ggcgcccgca ccgcatcgag caggtcgcca tgcgtggcct tcgacaggtt cttctccagc
     2401 gacgcaaggt tcgcctcgac atgccgcacc gactcggcca gcagtgcgcc gcctgcg
//
LOCUS       DASRDG010000595         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_663642, whole genome shotgun sequence.
ACCESSION   DASRDG010000595 DASRDG010000000
VERSION     DASRDG010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663642"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..865
                     /locus_tag="VFA35_09970"
     CDS             104..865
                     /locus_tag="VFA35_09970"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZT56543.1"
                     /translation="MRVIVTRPATQAADWVVQLRAHAIDAVALPLIGIAPVADPAALR
                     VAWRGLARQRLVVFVSPNAAEQFFAQRPLDAPWPPALLAGSPGPGTTRVLLGLGVPAE
                     RVVAPAGDALQFDSESLWAQMRDRDWRGANVLIVRGDGGRDWLAQTLREAGAHVAHLA
                     AYARAVPAFDAAESALLRAALARPAVHLWFFSSSEAIDNLAAIQDVGTDWAAAQAIAT
                     HPRIVARARQLGFARVTAARPSLPAVIACIQSIGS"
     gene            862..1998
                     /locus_tag="VFA35_09975"
     CDS             862..1998
                     /locus_tag="VFA35_09975"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZT56544.1"
                     /translation="MTEDSHNTSESGGAPAAALAVLPSAPARPAVPTRWFWAALAVLG
                     ALCVASLGLAWKADQRGGSLEQELVRRQQESAGRSTEARAFAKQAQESAGATAAKVAL
                     LEARVAEVAVQRGQLEDLIQSLSRSRDENLLVDIEAAIRVALQQNAITGSPEPLVATL
                     KQSDERLARYNQPRLEGVRRAIARDLDRVKATSVADIGSLGIKLDEAIRIVDDLPLLG
                     MADARSGKDAAKSPPAGKVSAAANAATPASASAGWGGRLIDTSNAFGEKVWSEVKSLV
                     RVTRIDHPEAMLLAPEQSFFVRENLKLRLLNARLALLSRQFDTAQSDLQAALTTLDRY
                     FERGSKRSVLATELIRQVAGQARQVVVPRPDDTLAALTAASAGR"
BASE COUNT          272 a          794 c          797 g          283 t
ORIGIN      
        1 cgcggcgacg gcggccgcga ctggctcgcg cagacgctgc gcgaggccgg cgccgcggcc
       61 tacctcggcg cggccggcgc ggcgccctga gcgcggtgcc gcgatgcgcg tgatcgtcac
      121 gcggccggcg acgcaggccg ccgactgggt ggtgcagctg cgggcccacg cgatcgacgc
      181 ggtcgcgctg ccgctgatcg gcatcgcgcc ggtcgccgac ccggccgcgc tgcgcgtcgc
      241 gtggcgcggg ctcgcgcggc agcggctggt cgtcttcgtc agcccgaacg cggccgagca
      301 gttcttcgcg cagcggccgc tcgatgcgcc ttggccgccc gcgctgctcg ccggctcgcc
      361 cggcccgggc acgacgcgcg tgctgctcgg cctgggcgtg ccggccgagc gcgtcgtcgc
      421 gccagcgggc gatgcgctgc agttcgactc cgaatcgctg tgggcgcaaa tgcgcgatcg
      481 cgactggcgt ggcgcgaatg tgctgatcgt gcgcggcgac ggcggccgcg actggctcgc
      541 gcagacgctg cgcgaggcag gcgcgcacgt ggcgcacctg gccgcctacg cgcgcgctgt
      601 gccggcgttc gacgcggccg aatcggcgct gctgcgcgct gcgctcgcgc ggccggcggt
      661 gcatctctgg ttcttcagca gttccgaggc gatcgacaac ctcgccgcga tacaggacgt
      721 gggcaccgac tgggccgccg cgcaggcgat cgccacgcat ccgcgcatcg tcgcgcgcgc
      781 gcggcaattg gggttcgcac gcgtgaccgc ggcgcgaccc tctctgccgg ccgtgatcgc
      841 ctgcatacaa tcgatcggat cgtgaccgaa gactcccaca acacctccga gtcgggcggc
      901 gcgccggccg ccgcgctggc cgtgctgccg agtgcgccgg cgcgcccggc cgtgccgaca
      961 cgctggttct gggccgcgct ggccgtgctc ggcgcgctgt gcgtcgccag cctggggttg
     1021 gcctggaagg ccgaccagcg cggcggcagc ctcgagcaag aactcgtgcg ccgccagcag
     1081 gagagcgcgg gcaggtcgac cgaggcgcgt gctttcgcca agcaggcgca ggagagcgcc
     1141 ggcgccaccg ccgccaaggt cgcgctgctc gaggcgcgcg ttgccgaggt cgcggtgcag
     1201 cgcggccagc tcgaagacct gatccagtcg ctgtcgcgct cgcgcgacga gaacctgctg
     1261 gtcgacatcg aggccgcgat ccgggtcgcg ctgcagcaga acgcgatcac cggcagcccc
     1321 gagccgctgg tcgccacgct gaagcagagc gacgagcgcc tcgcgcgcta caaccagcca
     1381 cgcctcgaag gcgtgcgccg tgcgatcgcg cgcgacctcg accgcgtcaa ggcgaccagc
     1441 gtggccgaca tcggctcgct cggcatcaag ctcgacgagg cgatccgcat cgtcgacgac
     1501 ctgccgctgc tggggatggc cgatgcgcgc agcggcaagg acgccgccaa gtcgccaccc
     1561 gccggcaagg tcagcgccgc ggcgaatgcc gcgacgccgg cgagcgcctc ggccggctgg
     1621 ggcgggcggc tgatcgacac ctcgaatgcg ttcggcgaga aggtctggag cgaggtcaag
     1681 tcgctcgtgc gcgtgacccg catcgaccac cccgaggcga tgctgctcgc gcccgagcaa
     1741 tcgttcttcg tgcgcgagaa cctgaagctg cggctgctga acgcgcggct cgcgctgctg
     1801 agccggcagt tcgacaccgc gcagtccgac ctgcaggcgg cgctgaccac gctcgatcgc
     1861 tacttcgagc gcggctcgaa gcgcagcgtg ctcgcgaccg agctgatccg ccaggtcgcg
     1921 ggccaagcgc gccaggtcgt cgtgccgcgg ccggacgaca cgctggcggc actgacggcc
     1981 gcgtcggccg gccgctaggg cccatgcgca acaccatctg gttcgtgctg ctgttcggcg
     2041 tggccgtgct cgccgcgacc acgctcggca ccaacgacgg cctggcgacc ttctactggc
     2101 gcggctggcg cgtcgacgtc tcgctgaact tgttcgtgct cgcgct
//
LOCUS       DASRDG010000596         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1475577, whole genome shotgun sequence.
ACCESSION   DASRDG010000596 DASRDG010000000
VERSION     DASRDG010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475577"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFA35_09980"
     CDS             <1..642
                     /locus_tag="VFA35_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZT56545.1"
                     /translation="PEVKFVRQVLRSTEVVWTDVFREAGKTYQLPKLTLYTGSYPTGC
                     GQGEAAMGPFYCPADAHVYLDMGFFNTMSHRLGAPGQFAQAYVIAHEIGHHVQNLLGI
                     SDKVDAMRRRGTEAQANAMSVRLELQADCFAGVWAKRSQSEQGWRLEQGDIETALNAA
                     SQIGDDTLQRKSRGTVVPESFTHGTSAQRVGWFKRGFESGSPDQCNTFEVRQL"
     gene            complement(653..1822)
                     /locus_tag="VFA35_09985"
     CDS             complement(653..1822)
                     /locus_tag="VFA35_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT56546.1"
                     /translation="MSGSALRRLGLGLPLAWRVYAAFFLYAFGMGGIFPRLAEIQRGL
                     GVAEGGFGLALIGTACGTLVSLTLAGRLLERIGHRRALLGLSALLPAFYALASHATGP
                     LALFVLLLPAGLCIGAIEIVVNLEADRVEHQTGRRFMNRAHAFWSVGFFVAGLFGAAL
                     SQLGVTPQWHLALVVPIEWAGLALLLGRFDAAPHRDTSAAAPPAARFARPTWGVIALV
                     AVALPALVLEGAGADWSAIYMRDAFGAPPFVAGLAVAIGAGAQALARFFADRFVEKHR
                     PVAVARVLLGVLGVGTLLVFKAPTGAVALLGFALMGVGTSAIFPLAMSAAAQRTDRAP
                     ATNVAALAQFAFVAFLLGPPLLGGVAQAFGIRWTFGVGLPLVGLSFAAAAALRSR"
     gene            complement(1819..2970)
                     /locus_tag="VFA35_09990"
     CDS             complement(1819..2970)
                     /locus_tag="VFA35_09990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZT56547.1"
                     /translation="MPVITHIEDLRVLAEKRVPRMFYDYADSGSYTESTYRANESDFQ
                     KIKLRQRVAVNMEGRSIRTRMAGVDTAMPVAIAPTGLTGMQHADGEILGALAASKFGI
                     PFTLSTMSICSIEDIAQATRAPFWFQLYWMRDRDFMDRLMDRAKAANVSALMLTLDLQ
                     VLGQRHKDIKNGMTAPPKPTLRNILNLMTKPRWCLGMAGTKRHGFGNLIGHAKGVSDM
                     SSLSTWTREQFDPKLDWNDVEWIKKRWGGKLILKGIMDEEDARLAVESGADAMVVSNH
                     GGRQLDGAPSSIEALPAITQAVGKMIEVWMDGGIRSGQDVLKAVALGAKGTLIGRAFL
                     YGLGAMGEAGVTQALEIIAKELDITMAFCGHTDIRKVDRGILLPGTLPG"
BASE COUNT          476 a         1072 c         1043 g          410 t
ORIGIN      
        1 cccgaggtga agttcgtgag gcaggtcctg cgcagcaccg aagtcgtctg gaccgacgtg
       61 ttccgcgagg cgggcaagac ttaccagttg cccaagctca cgctgtacac cggcagctac
      121 ccgaccggct gcggccaggg cgaagcggcg atgggcccgt tctattgccc ggccgatgcc
      181 cacgtctacc tcgacatggg cttcttcaac acgatgagcc accgcctggg tgcgcccggc
      241 cagttcgcgc aggcctacgt gatcgcgcac gagatcggcc accacgtgca gaacctgctc
      301 ggcatcagcg acaaggtcga tgcgatgcgt cggcgcggca ccgaagcgca ggccaacgcg
      361 atgagcgtgc ggctcgaact ccaggccgac tgcttcgcgg gcgtctgggc caagcgctcg
      421 cagtccgagc agggctggcg tctggagcag ggtgacatcg agaccgcgct gaacgccgcc
      481 tcgcagatcg gcgacgacac cttgcaacgc aaatcgcgcg gcacggtcgt gcccgagagc
      541 ttcacgcacg gcaccagcgc gcagcgcgtc ggctggttca agcgcggctt cgagtcgggc
      601 agcccggacc agtgcaacac cttcgaggtg cgccagctct gacggtctcg aatcaacggc
      661 tgcgcaacgc cgccgcggcg gcgaagctca gcccgaccag cggcagcccg acgccgaagg
      721 tccagcggat gccgaaggcc tgcgccacgc cgcccagcag cggcggcccg agcaggaacg
      781 cgacgaacgc gaactgcgcc agcgcggcca cgttggtggc cggcgcgcgg tcggtgcgct
      841 gcgccgcggc cgacatcgcg agcggaaaga tcgcgctcgt gcccacgccc atcagggcga
      901 agccgagcag cgcgaccgcg cccgtcggcg ccttgaacac cagcagcgtg ccgacgccga
      961 gcacgcccag cagcacccgc gcgacggcaa cgggccggtg cttctcgacg aagcggtcgg
     1021 cgaagaagcg cgcgagcgcc tgcgcgccgg caccgatcgc gaccgcgagg ccggcgacga
     1081 acggcggcgc gccgaaggca tcgcgcatgt agatcgccga ccagtcggcg cccgcgcctt
     1141 cgagcaccag cgccggcagc gccacagcga ccagcgctat cacgccccag gtcggccgcg
     1201 cgaagcgtgc cgccggcggc gcggcggcgc tggtgtcgcg gtgcggcgcg gcgtcgaagc
     1261 ggccgagcag cagcgccagg ccggcccatt cgatcggcac gacgagcgcg aggtgccatt
     1321 gcggcgtgac gccgagttgc gacagcgccg cgccgaacag cccggcgacg aagaagccga
     1381 cactccagaa ggcgtgcgcg cggttcatga agcgccggcc ggtctggtgc tcgacccggt
     1441 cggcctcgag gttgacgacg atctcgatcg cgccgatgca cagccccgcc ggcagcagca
     1501 gcacgaacag cgcgagcggc ccggtggcgt gcgacgcgag cgcgtagaac gccggcagca
     1561 gcgcgctcag cccgagcagc gcgcgccggt ggccaatgcg ctcgagcagc cgcccggcca
     1621 gcgtcagtga caccagcgtg ccgcaggcgg tgccgatcag cgcgaggccg aacccgccct
     1681 cggccacgcc gagcccgcgc tggatctcgg ccagccgcgg aaagatgccg cccatcccga
     1741 acgcgtagag aaagaacgcc gcgtacaccc gccacgcgag cggcaggccg agcccgaggc
     1801 gccgcagtgc ggagccgctc accccggcag cgtgcccggc agcaggatgc cgcggtcgac
     1861 cttgcggatg tcggtgtggc cgcagaacgc catcgtgatg tcgagctcct tcgcgatgat
     1921 ctcgagcgcc tgggtcacgc cggcctcgcc catcgcgccc agcccgtaga ggaacgcgcg
     1981 gccgatcagc gtgcctttgg cgccgagcgc gacggccttc agcacgtcct gccccgagcg
     2041 gatgccgccg tccatccaga cctcgatcat cttgccgacc gcctgcgtga tggccggcag
     2101 cgcctcgatc gacgagggcg cgccgtcgag ctggcggccg ccgtggttcg agaccaccat
     2161 cgcgtcggcg ccgctctcga ccgcaagacg agcgtcttcc tcgtccatga tgcccttcag
     2221 gatcagcttg ccaccccagc gcttcttgat ccactcgacg tcgttccagt cgagcttggg
     2281 gtcgaactgc tcgcgcgtcc aggtgctgag cgagctcatg tcgctgacgc ccttggcgtg
     2341 gccgatcagg ttgccgaagc cgtggcgctt cgtgccggcc atccccaggc accagcgcgg
     2401 cttggtcatc aggttcagga tgttgcgcag cgtgggtttc ggcggcgcgg tcatgccgtt
     2461 cttgatgtcc ttgtggcgct ggccgagcac ctgcaggtcc agcgtcagca tcagcgcgct
     2521 gacgttcgcg gccttcgcgc ggtccatcag ccggtccatg aagtcgcggt cgcgcatcca
     2581 gtagagctgg aaccagaacg gcgcacgcgt ggcctgcgcg atgtcctcga tcgagcagat
     2641 gctcatcgtg ctgagcgtga acgggatgcc gaacttgctt gccgcgagcg cgccgaggat
     2701 ctcgccgtcg gcgtgctgca tgccggtcag gccggtcggc gcgatcgcga ccggcatcgc
     2761 ggtgtcgacc cccgccatcc gggtgcggat cgaacgccct tccatgttca ccgcgacgcg
     2821 ctggcgcagc ttgatcttct ggaagtcgct ctcgttcgcg cggtaggtgc tttcggtata
     2881 ggagcccgag tcggcgtagt cgtagaacat gcgcggcacg cgcttctcgg ccagcacgcg
     2941 caggtcttcg atgtgggtga tcacgggcac gggggtctcc tgcttctgat tgcccgcgat
     3001 g
//
LOCUS       DASRDG010000597         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1244450, whole genome shotgun sequence.
ACCESSION   DASRDG010000597 DASRDG010000000
VERSION     DASRDG010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244450"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="VFA35_09995"
     CDS             complement(<1..1705)
                     /locus_tag="VFA35_09995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZT56548.1"
                     /translation="MTPAVTPAAPPIGAPISQLAAPAAPAVVAARAHVLTVSLEEYFH
                     GGALAKVVRRKHWDRFEPRLDRNVDQLLGALDKNSARATFFVLGLLADRNPALVRRIV
                     GAGHEVASRGFWPRGIADMQVEEFVEDLDRTALAISAAGGGRVRGFRSPRWLKREDLW
                     IVEVLGRKGYAYDSSVNPIGRSFAWMPEYHTVRRHEVPGVPGGIFEVPVSTASWFGFR
                     VAIAGGNWMRQLPPWLLARAVARWERQRPDPLVFYLTSWEFDPEQPLVTAASPWSRLR
                     HYRNLGRMRERLQHYLGQYRFVSIAEHLGLPALETAPAPAAGVQGPQAMIPLAPPPPA
                     PIGAVPVPVSVVVPMYQEQENIPYLMRTLATVSDRLAGRYRFEFVFVDDCSRDGTWEV
                     LQQSCRGRNDVKLLRHEKNRGVAAAIMTGIRAASNEIVCSIDCDCSYDPMEIQNMVPR
                     LGDGDLVTASPYHPDGQVLNVPRWRLLLSRTLSWIYRRVLGKDLFTWTSCFRVYRRSK
                     VVGMELQNGGFLGIAEMLVRMLRRGAVVREYPTLLESRLLGASKMKTMRTVRGHLGLL
                     WQ"
     gene            complement(1743..>2292)
                     /locus_tag="VFA35_10000"
     CDS             complement(1743..>2292)
                     /locus_tag="VFA35_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56549.1"
                     /translation="AIGFCAIGDGEGGNGLVGSLALLLGSRDKQVALVEATLRTPTLS
                     PLFEVSNEPGLADLLAGRGDLQDALRPQVAPGVDLVTGGSAADPFWGFTSDRFRALLG
                     ELLATRDLCLVDVPALNRAPEAALVIRALDAVVLVVEANRHRADVVRRNVAFLRSLGT
                     PFLGAVLTDLVHELPTRLARLC"
BASE COUNT          343 a          826 c          842 g          281 t
ORIGIN      
        1 cctgccacaa gagccccagg tgcccgcgca ccgtgcgcat cgtcttcatc ttcgacgcgc
       61 cgagcagccg cgactcgagc agcgtcgggt actcgcgcac gacggcgccg cggcgcagca
      121 tgcgcacgag catctcggcg atgccgagga agccgccgtt ctggagctcc atgccgacga
      181 ccttgctgcg ccggtagacg cggaagcagc tggtccaggt gaacaggtcc ttgccgagca
      241 cgcgccggta gatccacgac agcgtgcgcg acagcaacag gcgccagcgc ggcacgttga
      301 gcacctggcc gtccgggtgg tacggcgacg cggtgaccag gtcgccgtcg ccgagccgcg
      361 gcaccatgtt ctggatctcc atcgggtcgt agctgcagtc gcagtcgatc gagcagacga
      421 tctcgttcga cgccgcgcgg atgccggtca tgatcgcggc ggcgacgccg cggttcttct
      481 cgtggcgcag cagcttcacg tcattgcggc cgcggcagct ctgctgcagc acctcccagg
      541 tgccgtcgcg gctgcagtcg tcgacgaaca cgaactcgaa ccggtagcgg ccggcgagcc
      601 ggtcgctgac ggtcgcgagc gtgcgcatca ggtaggggat gttctcctgc tcctggtaca
      661 tcggcaccac caccgacacg ggcaccggca cggcgccgat cggcgccggc ggcggcggcg
      721 cgagcgggat catcgcctgc ggcccctgaa cgccggccgc gggcgccggc gccgtctcga
      781 gcgcgggcag gccgaggtgc tcggcgatcg agacgaaacg gtactggccg aggtagtgct
      841 gcagccgttc gcgcatgcgc ccgaggttgc ggtagtggcg caggcggctc cacggcgacg
      901 ccgcggtgac cagcggctgc tcggggtcga actcccacga cgtgaggtag aagaccagcg
      961 gatcgggccg ttgccgctcc caccgcgcaa ccgcgcgcgc gagcagccac ggcggcagct
     1021 gccgcatcca gttgccgccc gcgatcgcga cgcggaaacc gaaccacgac gccgtcgaca
     1081 cgggcacctc gaagatgccg cccggcacgc cgggcacctc gtggcgccgc acggtgtggt
     1141 actccggcat ccacgcgaac gagcgcccga tcgggttgac gctgctgtcg tacgcgtagc
     1201 ccttgcggcc gagcacctcg acgatccaca ggtcctcgcg cttgagccag cgcggcgagc
     1261 ggaacccgcg cacgcggccg ccgccggcgg cggagatcgc gagcgcggtg cggtcgaggt
     1321 cctcgacgaa ctcctcgacc tgcatgtcgg cgatgccgcg cggccagaag ccgcgcgagg
     1381 cgacctcgtg gccggcgccg acgatccggc gcacgagcgc cgggttgcgg tcggcgagca
     1441 gcccgagcac gaagaacgtc gcgcgcgccg agttcttgtc cagtgctccc agcagctggt
     1501 cgacgttgcg gtcgagccgc ggctcgaagc ggtcccagtg cttgcgccgc acgaccttcg
     1561 ccagcgcgcc gccgtggaag tactcctcca gcgacacggt gagcacgtgc gcgcgcgccg
     1621 cgaccaccgc gggcgccgcg ggcgcggcga gctgcgagat cggcgcgccg atcggagggg
     1681 cggccggcgt cacggctggc gtcactgggg gggctccagg gtccgcgggc acacgtgcag
     1741 tgtcagcaga ggcgcgccag gcgcgtcggc aattcgtgca ccaggtcggt caggaccgcg
     1801 ccgaggaacg gcgtacccag cgagcgcagg aacgcgacgt tccggcgcac cacgtcggcg
     1861 cggtggcggt tggcctccac caccagcacg accgcgtcga gtgcgcgaat caccagcgcc
     1921 gcctcgggcg cgcgattgag cgccggcacg tcgacgaggc agagatcgcg cgtcgccagc
     1981 agctcgccga gcagcgcacg gaagcggtcg ctcgtgaagc cccagaacgg atcggctgcg
     2041 ctgccgccgg tgacgaggtc gacgccgggc gcgacctgcg gccgcagcgc gtcctgcagg
     2101 tcgccgcggc cggcgagcag gtcggcgagg ccgggttcgt tcgagacctc gaacagcggc
     2161 gacagcgtcg gcgtgcgcag cgtcgcctcg accagcgcca cctgcttgtc gcggctgccg
     2221 aggagcagcg cgaggctgcc gacgaggccg ttgccgccct cgccgtcgcc gatcgcgcag
     2281 aaaccgatcg cg
//
LOCUS       DASRDG010000598         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_323692, whole genome shotgun sequence.
ACCESSION   DASRDG010000598 DASRDG010000000
VERSION     DASRDG010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323692"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VFA35_10005"
     CDS             complement(<1..1616)
                     /locus_tag="VFA35_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HZT56550.1"
                     /translation="MNHTASQLARPVLATLTFALLSGCAALKPAGGGNAAAAVQPSTT
                     PRPTAAAPGPAPSASGVPPGTPQLAPPPGSPAAFATVIRDAKKTEGLFTTYQKDEKVW
                     LELKPADFDKPFFLSPKLATGIGEARVFGGLMDQALVIEFRRIHNQVQMIARNTDYTA
                     RAGTPEGRAVAAAFSPSLLASTPVASQPQPDRKSVLVDLNALFVNDMLGIGMRLQRSY
                     RQGYAFDARNSAITALRVKPESVSLEVMQHFATGSIAVRQPNTPPGAPVPTVPATLAD
                     PRSLFVTAQYSISPLPAAPMHARPADARVGYFTATYWDFSDDLSRTPVVRHVNRWRLE
                     KKDPAAALSEPVKPIVYVLDRTIPLKYRDTITAGILEWNKAFEKIGFKNAIQVKVQAD
                     DADVDTLDGGASVRWMTNAAPSFGAIGPSHVDPRSGEILAANIGIESLSSRNQRATRA
                     QILNGSPADLARLMQFGGAASGDAFDARACLNADQAADQLGYALDVLAARGDVDPDSP
                     EAQAYVLAYLKDVTMHEVGHTLGLRHNFRAS"
     gene            complement(1693..>2939)
                     /gene="ettA"
                     /locus_tag="VFA35_10010"
     CDS             complement(1693..>2939)
                     /gene="ettA"
                     /locus_tag="VFA35_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZT56551.1"
                     /translation="DRTLEIAMDALRLPPAEATVANLSGGEKRRVALCRLLLSKPDML
                     LLDEPTNHLDAESVDWLEQFLQRYPGTVVAITHDRYFLDNAAEWILELDRGRGIPYKG
                     NYSTWLDQKEQRLEQEQKTEDARTKAMKKELEWVRANPKGRQAKSKARLARFEELSDI
                     DYQKRNETNEIFIPVGERLGHEVLEFENVTKSFGDRVLIDNLSFKVPAGAIVGIIGPN
                     GAGKSTLFRMISGKETPDSGTVKIGPTVKMAFVDQSREDLQADKTVWQDVSGGLDNIV
                     VGKFVMPSRAYIGKFNFKGNDQQKLVGQLSGGERGRLHLAKTLAQGGNVLMLDEPSND
                     LDVETLRALEDALLEFAGSLMVISHDRWFLDRIATHILACEGDSKWVFFDGNYQEYEA
                     DKKKRLGEEGARPHRLRYKALK"
BASE COUNT          401 a          959 c         1051 g          528 t
ORIGIN      
        1 gacgcgcgga agttgtggcg caggccgagc gtgtggccga cctcatgcat cgtcacgtcc
       61 ttcaggtacg cgagcacgta ggcctgcgcc tcggggctgt cggggtcgac gtcgccgcgc
      121 gcggcgagca cgtcgagcgc gtagccgagc tggtcggcgg cctggtcggc gttcaggcag
      181 gcacgcgcgt cgaaggcgtc gccgctggcg gcaccgccga actgcatcag ccgcgccagg
      241 tcggccgggc tgccgttcag aatctgcgcg cgcgtcgcgc gctggttgcg cgacgacaag
      301 ctctcgatgc cgatgttggc cgccaggatt tcgccgctgc gcgggtcgac atggctcggg
      361 ccgatcgcgc cgaaggacgg cgcggcgttg gtcatccaac gcaccgaggc gccgccgtcg
      421 agcgtgtcga catcggcgtc gtcggcctgc accttgactt gaatcgcgtt cttgaagccg
      481 atcttctcga aggccttgtt ccactcgagg atgccggcgg tgatcgtgtc acggtacttc
      541 agcgggatcg tgcggtcgag cacgtagacg atcggcttga ccggctcgga cagcgcggcc
      601 gccgggtcct tcttctcgag gcgccagcgg ttcacgtggc gcacgaccgg ggtgcgggac
      661 aggtcgtcgc tgaaatccca gtaggtcgcg gtgaagtagc cgacgcgcgc gtcggccgga
      721 cgcgcgtgca tcggtgcggc cggcagcggc gagatcgaat actgcgcggt cacgaacagg
      781 ctgcgcgggt cggccagcgt cgccggcacg gtcggcacgg gcgcgccggg cggcgtgttc
      841 ggctggcgca ccgcgatcga gccggtcgcg aaatgctgca tcacttcgag cgacacgctc
      901 tcgggcttga cgcgcagcgc agtgatcgcc gagttgcgcg cgtcgaacgc gtagccctgc
      961 cggtagctgc gctgcagtcg catgccgatg ccgagcatgt cgttgacgaa cagcgcgttc
     1021 aggtccacca gcaccgactt gcgatcgggc tgcggctggc tcgccaccgg cgtgctcgcg
     1081 agcaggctgg gcgagaacgc cgcggcgacc gcgcggccct cgggcgtgcc ggcgcgcgcc
     1141 gtgtagtcgg tgttgcgcgc gatcatctgg acctggttgt ggatgcgccg gaattcgatc
     1201 accagcgcct ggtccatcag gccgccgaac acgcgcgcct cgccgatgcc ggtggcgagc
     1261 ttcggcgaca ggaagaacgg cttgtcgaag tcggccggct tcagctcgag ccagaccttc
     1321 tcgtccttct ggtaggtcgt gaacaagcct tcggtcttct tcgcgtcgcg gatcacggtc
     1381 gcgaacgccg ccgggctgcc cggcggcggt gccagttgcg gcgtgccggg cggcacgccc
     1441 gaggcgctcg gcgccgggcc tggggccgct gcggtcgggc gcggcgtggt cgatggttgc
     1501 accgccgcgg ccgcgttgcc gccgcccgcg ggcttcagcg cggcgcagcc gctcagcaag
     1561 gcgaaggtca gcgtggccag cacgggccgg gcgagttggg acgcagtgtg gttcatcggt
     1621 gcgcggtggg ctgggaaact gattctgcca gcggcgtgta aacccccgct ggcagcttgt
     1681 cacatcagcg tgtcacttca gcgccttgta gcgcaggcgg tgcgggcggg cgccctcttc
     1741 gccgaggcgc ttcttcttgt cggcctcata ttcctgatag ttgccatcga agaagaccca
     1801 cttcgagtcg ccctcgcagg ccaggatgtg ggtggcgatg cggtcgagaa accaacggtc
     1861 gtggctgatc accatcagcg agcccgcgaa ctcgagcagc gcgtcttcga gcgcacgcag
     1921 ggtttcgacg tcgaggtcgt tgctcggttc gtcgagcatc agcacgttgc cgccctgcgc
     1981 cagcgtcttc gccaggtgca gccggccgcg ctcgccgccc gagagctggc cgaccagctt
     2041 ttgctggtcg ttgcccttga agttgaactt gccgatgtag gcgcgcgacg gcatcacgaa
     2101 cttgccgacg acgatgttgt ccaggccgcc ggaaacgtcc tgccacacgg tcttgtcggc
     2161 ctgcagatcc tcgcggctct ggtcgacgaa ggccatcttc acggtcgggc cgatcttcac
     2221 ggtgcccgag tcgggcgtct ccttgcccga gatcatccgg aacagcgtcg acttgccggc
     2281 gccgttcggc ccaatgatgc cgacgatcgc gccggccggc accttgaagc tgaggttgtc
     2341 gatcagcacg cggtcgccga agctcttcgt gacgttctcg aactcgagca cctcgtgacc
     2401 gaggcgctcg ccgaccggga tgaagatctc gttggtctcg ttgcgcttct ggtagtcgat
     2461 gtcgctcagt tcctcgaagc gcgccaggcg cgccttgctc ttcgcctggc ggcccttcgg
     2521 gttcgcgcgc acccattcga gctctttctt catcgccttg gtgcgggcgt cttcggtctt
     2581 ctgctcctgc tcgaggcgct gctccttctg atcgagccag gtcgagtagt tgcccttgta
     2641 agggatgccg cgaccgcggt cgagttcgag aatccactcg gccgcgttgt cgaggaagta
     2701 gcggtcgtgg gtgatcgcga cgaccgtgcc cggatagcgc tgcaggaact gctcgagcca
     2761 gtcgacgctt tcggcgtcga ggtggttggt cggctcgtcg agcagcagca tgtcgggctt
     2821 gctcaacaag agccggcaca gcgcgacgcg gcgcttctcg ccgcccgaga ggttggcgac
     2881 ggtggcctcg gcgggcggga gccgcagcgc atccatggcg atctcgaggg tgcggtcga
//
LOCUS       DASRDG010000599         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_896112, whole genome shotgun sequence.
ACCESSION   DASRDG010000599 DASRDG010000000
VERSION     DASRDG010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896112"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VFA35_10015"
     CDS             complement(<1..1114)
                     /locus_tag="VFA35_10015"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HZT56552.1"
                     /translation="MSSPARSLFAAVEMAPRDPILGLNEQFNADPNPAKVNLGVGVYF
                     DDNGKLPLLDCVQAAEKMMMEAPKARGYLPIDGIAAYDKAVQGLVFGADSAAVKAGRI
                     ATVQAVGGTGGLKVGADFLKRLNPGATVLISDPSWENHRALFEGAGFPVAQYPYYDAA
                     KHGVDVDGMLAALDKAAAGTIVVLHACCHNPTGYDITPDQWAKVIATIKARNLVPFLD
                     MAYQGFGEGIQEDGAVVAQFMAAGLDFFVATSFSKSFSLYGERVGALSVVCQSKEEAD
                     RVLSQLKRVIRTNYSNPPTHGAQVVALVLTTPALRTMWEGELAAMRVRIKEMRVALRA
                     KLEAAGLKQDVSFITRQKGMFSYSGLTKPQMERLRSE"
     gene            1202..3322
                     /gene="uvrB"
                     /locus_tag="VFA35_10020"
     CDS             1202..3322
                     /gene="uvrB"
                     /locus_tag="VFA35_10020"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZT56553.1"
                     /translation="MAELSEIIDPALGAAEPEGRFVTYPGSPFQLFQPYPPAGDQPSA
                     IAGLVEGLNDGLSFQTLLGVTGSGKTFTMANVIAQMGRPAIVFAPNKTLAAQLYAEFR
                     EFFPKNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSLMERRDV
                     VIVASVSAIYGIGDPSAYHQMVMTLRVHDKLGQRDVIAHLIRMQYTRNEVDFSRGTFR
                     VRGDTIDVFPAEHSELAIRIELFDDEIESLQLFDPLTGRIRQKLPRFTVYPSSHYVTP
                     RERVVSAIDTIKAELRERLDELVKNGKLVEAQRLEQRTRFDLEMLQEVGHCKGIENYT
                     RHLSGAAPGDPPPTLVDYLPADALMFLDESHVLIGQFGGMYNGDRARKTTLVEYGFRL
                     PSALDNRPLKFEEFEKKMRQVMYVSATPAAYEQRTAGQVVEQLVRPTGLVDPKVEVRP
                     ASNQVDDVLQEIRERVEAGERVLITTLTKRMAEQLTDYLSDNGVKVRYLHSDVDTVER
                     VEILRDLRLGAFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAA
                     RNLNGRAILYADKVTESMRKAIDETERRRARQIAHNERLGITPRSVVKKVRDLIDGVY
                     SEKGAKDDLKSAVESAATLALSEKDLSKRIKLLEKQMLDHARNLEFEKAARVRDQLAQ
                     LREQAFGAPGTDNVVALAPNRPER"
BASE COUNT          606 a         1117 c         1162 g          548 t
ORIGIN      
        1 attcgctgcg caggcgttcc atttgcggct tggtcaggcc cgagtagctg aacatgccct
       61 tctggcgcgt gatgaagctc acatcctgct tcagccccgc ggcttcgagc ttggcccgca
      121 gcgcgacgcg catttccttg atgcgcacgc gcatcgcggc gagttcgcct tcccacatcg
      181 tgcgcagcgc gggcgtcgtg agcaccagcg cgacgacttg cgcgccgtgc gtcggcgggt
      241 tcgagtagtt ggtgcggatg acgcgcttga gctggctcag cacgcggtcg gcctcttcct
      301 tcgattgaca gacgacgctg agcgcgccga cgcgttcgcc gtagagcgag aagctcttcg
      361 agaaactggt cgcgacgaag aagtcgagcc cggcggccat gaactgcgcg acgaccgcgc
      421 cgtcttcctg gatgccctcg ccgaagccct ggtaggccat gtcgaggaag ggcaccagat
      481 tgcgcgcctt gatcgtggcg atcaccttgg cccattggtc gggcgtgatg tcgtagccgg
      541 tcgggttgtg gcagcaggcg tgcagcacga cgatggtgcc ggccgcggcc ttgtcgagcg
      601 cggcgagcat gccgtccacg tcgacgccgt gcttggccgc gtcgtagtac gggtactggg
      661 cgaccgggaa accggcgccc tcgaacagcg cgcggtggtt ctcccagctc gggtcgctga
      721 tcagcacggt ggcgccgggg ttcaggcgct tcaggaagtc ggcgccgacc ttcagcccgc
      781 cggtgccgcc gacggcttgc acggtcgcga tgcggccggc cttgaccgcc gcgctgtcgg
      841 cgccgaacac caggccttgc acggccttgt cgtaggcagc gatgccgtcg atcggcagat
      901 agccccttgc cttcggcgct tccatcatca tcttctcggc ggcctggacg cagtcgagca
      961 gcgggagctt gccgttgtcg tcgaagtaga cgccgacgcc caggttgacc ttcgccgggt
     1021 tcggatcggc gttgaactgc tcgttcaggc ccaggatcgg gtcgcggggg gccatttcga
     1081 cggcggcaaa cagtgaacga gcgggcgaag acatggtcgg tccttgtcag tagaggggcg
     1141 caggcgattc cgttaacctg cgcggttgct taacggggaa aacccggaga ttttaactgc
     1201 catggccgag ctgtccgaaa tcatcgatcc cgcgctcggc gctgccgagc ccgagggcag
     1261 gttcgtcacc tatcccggtt cgccgttcca gctgttccag ccgtacccgc cggcgggcga
     1321 tcagccgagc gcaatcgctg gcctggtcga aggcctgaac gacgggctga gcttccagac
     1381 gctgctcggc gtcaccggct cgggcaagac cttcacgatg gcgaacgtga tcgcgcagat
     1441 gggccggccg gcgatcgtgt tcgcgccgaa caagacactg gcggcgcagc tttacgccga
     1501 gttccgcgag ttcttcccga agaacgcggt cgagtacttc gtcagctact acgactacta
     1561 ccagcccgag gcctacgtgc cgcagcgcga cctgttcatc gagaaggaca gctcgatcaa
     1621 cgagcacatc gagcagatgc gcctgtcggc gacgaagagc ctgatggagc gccgcgacgt
     1681 ggtcatcgtc gcgagcgtct cggcgatcta cggcatcggc gaccccagcg cctaccacca
     1741 gatggtgatg acactgcgcg tgcacgacaa gctgggccag cgcgacgtga tcgcgcacct
     1801 gatccgcatg cagtacacgc gcaacgaggt cgatttctcg cgcggcacgt tccgcgtgcg
     1861 cggcgacacg atcgacgtgt tcccggccga gcactcggag ctggcgattc gcatcgagct
     1921 gttcgacgac gagatcgaat cgctgcagct gttcgacccg ctcaccgggc gcattcgcca
     1981 gaagctcccg cgcttcacgg tctatccgag cagccactac gtgacgccgc gcgagcgcgt
     2041 ggtgagcgcg atcgacacca tcaaagccga gttgcgcgag cggctcgacg aactcgtgaa
     2101 gaacggcaag ctggtcgagg cgcagcggct cgagcagcgc acgcgcttcg atctcgagat
     2161 gctgcaggag gtgggccact gcaagggcat cgagaactac acgcggcacc tgtcgggcgc
     2221 ggcgccgggc gacccgccgc cgacgctggt cgactacctg ccggccgacg cgctgatgtt
     2281 cctcgacgag agccatgtgc tgatcggcca gttcggcggc atgtacaacg gcgaccgggc
     2341 gcgcaagaca acgctggtcg aatacggctt tcggctgccg agcgcgctcg acaaccggcc
     2401 gctgaagttc gaggagttcg agaagaagat gcgccaggtg atgtacgtct cggcgacgcc
     2461 ggcagcctac gagcagcgga ccgccgggca agtcgtcgag cagctcgtgc gcccgaccgg
     2521 gctggtcgat ccgaaggtcg aggtgcgccc ggcgagcaac caggtcgatg acgtgctgca
     2581 ggagatccgc gagcgcgtcg aggcgggcga gcgggtcctc atcacgacgc tgaccaagcg
     2641 catggccgag cagctcaccg actacctcag cgacaacggc gtcaaggtgc gctacctgca
     2701 cagcgatgtc gacacggtcg agcgggtcga gatcctgcgc gacctgcggc tcggcgcttt
     2761 cgacgtgctg gtcggcatca acctgctgcg cgaggggctg gacatccccg aggtctcgct
     2821 ggtcgcgatc ctcgacgccg acaaggaagg ctttctgcgc tccgagcgca gcctgatcca
     2881 gaccatcggc cgcgcggcgc gcaacctgaa cggccgcgcg atcctgtatg ccgacaaggt
     2941 gaccgagtcg atgcgcaagg cgatcgacga gaccgagcgg cgccgcgccc ggcagatcgc
     3001 gcacaacgag cggctcggca tcacgccgcg cagcgtcgtc aagaaggtgc gcgacctgat
     3061 cgacggcgtc tacagcgaga agggcgcgaa ggacgacctg aagtcggcgg tcgagtccgc
     3121 agcgaccctg gcactgagcg agaaggacct gagcaagcgc atcaaactgc tcgaaaagca
     3181 gatgctcgac cacgctcgca acctcgaatt cgaaaaggcg gcgcgggtgc gcgaccagct
     3241 ggcgcagctg cgcgagcaag ccttcggcgc gccgggcacc gacaacgtcg tcgcgctcgc
     3301 gccgaatcgg cccgaacgct gaggccggaa acgcatttcc ctcgcgaccg aagggaactt
     3361 ccccggccaa tctcgaccga atttgtcggc ttcggctata cttgacggaa ctagtcggaa
     3421 tagggcgggt tta
//
LOCUS       DASRDG010000600         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_800854, whole genome shotgun sequence.
ACCESSION   DASRDG010000600 DASRDG010000000
VERSION     DASRDG010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800854"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="carB"
                     /locus_tag="VFA35_10025"
     CDS             <1..616
                     /gene="carB"
                     /locus_tag="VFA35_10025"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828861.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HZT56554.1"
                     /translation="AQKALGGRPPREIVPPYYSVKEAVFPFNKFPGVDPILGPEMRST
                     GEVMGSGRSFGEAMLKSQLGAGSRLPTSGAVCITVKNGDKARAVVVARDLHALGFALV
                     ATKGTAAAIAAAGVPVKAVNKVKDGRPHIVDMVKAGEIQLVFTTVDETRTAIADSRHI
                     RQAALAHRITYYTSMAGCEAAVEGMKHEDDLVVLSLQELHRELH"
     gene            690..1166
                     /gene="greA"
                     /locus_tag="VFA35_10030"
     CDS             690..1166
                     /gene="greA"
                     /locus_tag="VFA35_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZT56555.1"
                     /translation="MATIPLTVRGAEKLKEELARLKNVERHAVIQAIAEARAQGDLSE
                     NAEYEAAKDKQGFIEGRILEIESKLAAAQIIDPRTLNAEGRVVFASTVDLEDEDSGVA
                     VTYQIVGDDEADLKHGLISISSPIARALIGKSAGDVAEVQAPGGVRNYEIIAVRYG"
     gene            1189..1656
                     /locus_tag="VFA35_10035"
     CDS             1189..1656
                     /locus_tag="VFA35_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HZT56556.1"
                     /translation="MNFARFAALIAGLWAGVLLCIGAIAAPAAFAALAPTDAGRVAGR
                     MFAIEAYLSLAVAVVLCVIERQRSRDAAGTGSVFSADLMLLLGTLFCTVAGYFAVQPM
                     MEAARAGQGAVSLGTLHAISAGLFVLKGLLVLALAWRLNAMTPTAVSRTATTS"
     gene            complement(1631..2107)
                     /locus_tag="VFA35_10040"
     CDS             complement(1631..2107)
                     /locus_tag="VFA35_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HZT56557.1"
                     /translation="MTAIQLTPAQRKEHRAEAHHLDPVVMIGADGLTPAVQKEVDAAL
                     SAHGLIKVRVLSDERATRETIFTTLADALGAAPIQHIGKLLVLWRPLPPKEKAERAGR
                     GAGPRVVKILKFSKSGNHRAQVKKITVMGNMRIAAGGELKRATKRRSSVKKSSQSD"
     gene            2128..2838
                     /locus_tag="VFA35_10045"
     CDS             2128..2838
                     /locus_tag="VFA35_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HZT56558.1"
                     /translation="MKTQTKSKKVNKQWLHDHMNDTYVKLAQREGYRARAAYKLKEID
                     EELHLIKPGQLVVDLGSVPGAWSQYVRRRFAPKEVGAGGAAAGELNGTIIALDVLAMA
                     PIEGVTFLQGDIREEAVLAQLEEIVAGRPVDVVVSDMAPNLSGIESADSARISHLVEL
                     AVDFARAHLAPQGALVCKVFHGSGYSQLVKLFKQTFRVVKPIKPKASRDKSSETFLVG
                     IGLRPDAQARPVPADIAS"
BASE COUNT          455 a          958 c         1006 g          461 t
ORIGIN      
        1 cgcacagaag gcgctgggcg ggcggccgcc gcgcgagatc gtgccgccct actactcggt
       61 caaggaagcg gtcttcccgt tcaacaagtt ccccggcgtc gacccgatcc tcggacccga
      121 gatgcgctcc accggcgagg tgatgggctc cggccgcagc ttcggcgagg cgatgctgaa
      181 gagccagctc ggcgccggct cgcggctgcc gacctcgggc gccgtctgca tcaccgtgaa
      241 gaacggcgac aaggcgcgcg cggtcgtggt tgcgcgcgac ctgcacgcgc tcggcttcgc
      301 gctcgtcgcg accaaaggca cggccgcggc gatcgcggcg gccggcgtgc cggtcaaggc
      361 ggtcaacaag gtcaaggacg gccggccgca catcgtcgac atggtcaagg ccggcgagat
      421 ccagctcgtg ttcacgacgg tcgacgaaac ccgcaccgcg atcgccgact cgcgccacat
      481 ccgccaggcg gcgctggccc atcgcatcac ctactacacc agcatggccg ggtgcgaagc
      541 ggcggtcgaa gggatgaagc acgaagacga cctcgtcgtg ttgtctctgc aggaactgca
      601 tcgcgaactg cactaaactc cgcgccatca gtaccgccgc cggctaatcc cggcggcggc
      661 gttatttttg cgatcgaggt ttgctcatca tggccaccat tcccctcacg gtccgcggcg
      721 ccgagaagct caaggaagag cttgcccggc tgaagaacgt cgaacgtcac gccgtcatcc
      781 aggccatcgc cgaggcgcgc gcgcagggcg acttgtcgga gaacgccgag tacgaggcgg
      841 cgaaggacaa gcagggcttc atcgaaggcc gcatcctcga gatcgagagc aagctcgcgg
      901 ccgcgcagat catcgacccg cggacgttga acgccgaggg ccgcgtcgtg ttcgcctcga
      961 cggtcgacct ggaagacgaa gacagcggcg tcgcggtgac ctaccagatc gtcggcgacg
     1021 acgaggccga tctgaaacac ggcctgatct cgatcagctc gccgatcgcg cgggcgctga
     1081 tcggcaagtc ggccggcgac gtggccgagg tgcaggcgcc cggcggcgtg cgcaactacg
     1141 aaatcatcgc ggtccggtat gggtgagccg gggccgggtg cggtggcagt gaacttcgct
     1201 cgcttcgccg ccctgatcgc cgggctgtgg gccggggtgc tgctgtgcat cggcgccatc
     1261 gcggcaccgg ccgcgtttgc ggcgctcgcg ccgaccgatg cggggcgggt ggccggtcgc
     1321 atgtttgcga tcgaggccta cctgagtctg gcggtcgcgg tggtcctgtg tgtcatcgaa
     1381 cgccagcgca gccgcgacgc cgcgggcacc ggctcggtgt tcagcgccga cctgatgctg
     1441 ctgctcggca ccctgttctg cacggttgct ggctacttcg cggtgcaacc gatgatggag
     1501 gccgcgcgcg ccgggcaggg cgccgtctcg ttgggcacgc tgcacgcgat ttcggccggc
     1561 ctcttcgtgc tgaagggcct gctcgtgctg gcgctggcgt ggcgcctgaa cgccatgacg
     1621 ccaacggcag tcagtcggac tgcgacgact tcttgacgct gctgcggcgc ttcgtcgccc
     1681 gcttcagctc gccgccagcg gcaatacgca tgttgcccat caccgtgatc ttcttgacct
     1741 gtgcgcggtg gttgccgctc ttcgagaact tcaggatttt cacgacccgc gggcctgcgc
     1801 cgcggccggc gcgttcggcc ttctccttcg gcggcagcgg ccgccacagc accagcagct
     1861 tgccgatgtg ctggatcggc gcggcgccga gcgcgtcggc cagcgtcgtg aagatcgttt
     1921 cgcgcgtggc gcgttcatcg gagagcacac gcaccttgat caggccgtgg gcactgaggg
     1981 ccgcgtccac ttccttctgc acggccggcg tcagcccgtc ggcgccgatc atcaccaccg
     2041 ggtcgaggtg atgggcttcg gcgcggtgtt ccttgcgctg ggcgggagtg agttggatcg
     2101 cggtcatccc tctattatcg ccaccagatg aagacccaga ccaaaagcaa gaaggtgaac
     2161 aagcagtggt tgcacgacca catgaacgac acctacgtga agctggcgca gcgcgagggc
     2221 tatcgggccc gcgccgcgta caagctgaag gagatcgacg aggaactgca cctgatcaag
     2281 ccgggccagc tggtcgtcga cctgggctcg gtgcccggcg cctggagcca gtacgtgcgc
     2341 cggcgtttcg cgccgaagga ggtcggcgcc ggcggcgcgg cggccggcga actgaacggc
     2401 acgatcatcg cgctcgacgt gctggcgatg gcgccgatcg agggtgtgac cttcctgcag
     2461 ggcgacatcc gcgaggaggc ggtgctggcg cagctcgaag agatcgtcgc cgggcggccg
     2521 gtcgacgtcg tcgtctccga catggcgccg aatctgtcgg gcatcgaaag cgccgactcg
     2581 gcgcgcatca gccacctggt cgagctggcg gtcgacttcg cccgcgcgca cctcgcaccg
     2641 cagggcgcgc tggtgtgcaa ggtgttccat ggcagcggct acagccagct ggtgaagctg
     2701 ttcaagcaga ccttccgggt ggtcaagccg atcaagccga aggcctcgcg cgacaagtcg
     2761 tccgagacct ttctggtcgg gatcgggctg aggcccgatg ctcaggcccg ccctgttcct
     2821 gccgatatcg cttcctgagg gccggtcgcg agggtcgaac agctcgatac acgcccgcgg
//
LOCUS       DASRDG010000601         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1353558, whole genome shotgun sequence.
ACCESSION   DASRDG010000601 DASRDG010000000
VERSION     DASRDG010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353558"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VFA35_10050"
     CDS             complement(<1..996)
                     /locus_tag="VFA35_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZT56559.1"
                     /translation="MAVNPFGSDWLAGGRGERLRPQGTLSYVSGSTDEPLRFITLSQQ
                     LDKTVSRHGPRDAAIFDAEELRLSWYDLKQRADEMAAGLLALGLRRGNRVGIWAPNRH
                     EWIVTQFATARIGLVLVNINPAYRKTELEYALAKVGCRALVMARSFKGSDWLGMLGEI
                     APEIHLKGASEALDSVHLPELKHVVLLGDEPVPPRCLSYRRLLALAGPAQRARLDALS
                     GALDPDDAINIQFTSGTTGSPKGATLTHFNIVNNARYCAKAIALGPDDRLCIPVPLYH
                     CFGMVLGVLCATASGATMVFPGESFDAGETLRSIARNGCTALHGVPTMFGAMLEHD"
     gene            complement(1025..>2182)
                     /locus_tag="VFA35_10055"
     CDS             complement(1025..>2182)
                     /locus_tag="VFA35_10055"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZT56560.1"
                     /translation="VDATVATRILDAGGTIAGKAHCEYFCLSGGSHTSASGPVHNPYK
                     LGYSAGGSSSGCAALVGAGEIEMAIGGDQGGSVRMPGSFSGVYGMKPTHGLVPYSGAM
                     PIEATIDHLGPMTGTVADNALLLEVIAGADGLDPRQYNVRVDKYTAALGRGVAGLRIG
                     VLTEGFEFESCEPDVVQKVRQAAERLRSLGAIVEEVSIPMHVDGLPIWTPIALEGLVA
                     QMMHGNGMGFNWEGLYTTSLLDAHANWRARANQLSRTLKVSMMAGEYFIRQYRGHFYA
                     KAQNLSRLLKKTYDQAFARHELLLMPTTPMKATPIPAQDAPLALYCQRAFEMLPNTAP
                     FDVSGHPAMNVPCGLSAGLPVGMQLVGAHYAESTIYRAAHAFEQLGDWRNF"
BASE COUNT          314 a          741 c          786 g          341 t
ORIGIN      
        1 gtcgtgttcg agcattgcgc cgaacatcgt cggcacaccg tgcagcgccg tgcagccatt
       61 cctcgcgatc gagcgcagcg tctcgccggc gtcgaagctc tcgccaggga agaccatggt
      121 cgcgcccgag gcggtggcgc agagcacgcc gagcaccatg ccgaagcagt ggtagagcgg
      181 caccgggatg cagagccggt cgtcggggcc cagcgctatg gccttggcgc agtagcgcgc
      241 gttgttgacg atgttgaagt gggtgagcgt tgcccccttc ggcgacccgg tcgttccgct
      301 cgtgaactgg atgttgatcg cgtcgtcggg gtcgagcgcg ccgctcaggg cgtcgagccg
      361 cgcgcgctgc gctgggccgg caagcgcgag cagccggcgg tacgataggc agcgcggcgg
      421 caccggctcg tcgccgagca ggacgacgtg cttgagctcg ggcaggtgga cgctgtccag
      481 cgcctcgctc gcgcccttga gatggatctc gggggcgatc tcgccgagca tgccgagcca
      541 gtcgctgccc ttgaagcttc gcgccatgac gagcgcacgg cagccgacct tggcgagcgc
      601 gtactcgagc tcggtcttgc ggtacgccgg attgatgttg accaggacga gaccgatccg
      661 cgccgtcgcg aactgggtga cgatccactc gtgccggttc ggcgcccaga tgccgacgcg
      721 gttgccgcgc cgcaggccga gcgcgagcag gcccgcggcc atctcgtcgg cgcgctgctt
      781 caggtcgtac catgacaggc gcagctcctc ggcgtcgaag atcgctgcgt cgcgcgggcc
      841 gtggcgggaa acggtcttgt cgagctgttg cgacagcgtg atgaagcgga gcggctcgtc
      901 ggtcgagccg gacacgtacg agagcgttcc ctgcggcctc agccgctcgc cgcggccgcc
      961 ggcgagccag tcggagccga acggattgac cgccatggct gtcctcgcgg gcgctcgagc
     1021 cgcgctagaa gttgcgccag tcgccgagct gctcgaaggc gtgcgcggcg cggtagatcg
     1081 tcgactcggc gtagtgcgcg ccgaccagct gcatgccgac cggcagcccc gccgacaggc
     1141 cgcagggcac gttcatcgcc gggtggccgc tgacgtcgaa cggcgcggta ttgggcagca
     1201 tctcgaaggc gcgctggcag tagagcgcga gcggcgcgtc ctgtgccggg atcggcgtcg
     1261 ccttcatcgg cgtcgtcggc atcagcaaca gctcgtggcg cgcgaacgcc tggtcgtagg
     1321 tcttcttcag caggcgcgac aggttctgcg ccttggcata gaagtgaccg cggtactgac
     1381 ggatgaagta ctcgccggcc atcatcgaga ccttgagcgt gcgcgaaagc tggttcgcgc
     1441 gcgcgcgcca gttggcgtgc gcgtcgagca ggctcgtcgt gtacaggccc tcccaattga
     1501 agcccatgcc gttgccgtgc atcatctgcg ccaccaggcc ttcgagcgcg atcggcgtcc
     1561 agatcggcag gccgtcgacg tgcatcggga tcgacacctc ctcgacgatc gcgcccaggc
     1621 tcctcagccg ctccgccgcc tgacgcacct tctgcacgac gtcgggctcg cacgactcga
     1681 actcgaagcc ttcggtgagc acgccgatgc gtagccccgc gacgccgcgg ccgagcgcgg
     1741 ccgtgtactt gtcgacgcgg acgttgtact ggcgcgggtc caggccgtcg gccccggcga
     1801 tcacctcgag cagaagggcg ttgtcggcga cggtgccggt catcggcccg aggtggtcga
     1861 tcgtcgcctc gatcggcatc gcgccgctgt agggaaccag gccgtgcgtc ggcttcatgc
     1921 cgtagacgcc gctgaacgat cccggcatgc gcaccgagcc gccctggtcg ccgccgatcg
     1981 ccatctcgat ctcgcccgcg ccgacgagcg cggcgcagcc gctcgacgag ccgccggccg
     2041 agtagccgag cttgtacggg ttgtgcaccg gcccggacgc cgaggtgtgg ctgccgccgg
     2101 acaggcagaa gtactcgcag tgcgccttac cggcgatcgt gccgccggcg tcgaggatgc
     2161 gcgtggcgac cgtggcgtcg ac
//
LOCUS       DASRDG010000602         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_664429, whole genome shotgun sequence.
ACCESSION   DASRDG010000602 DASRDG010000000
VERSION     DASRDG010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664429"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="VFA35_10060"
     CDS             complement(<1..1401)
                     /locus_tag="VFA35_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56561.1"
                     /translation="MPQRVPRAAQRIVADIDLAVSASMGRNGCGCETAFSHRRVICAA
                     RLLWPVALAYHAGSILGRIPMKSLIACAAIAICAHAAASVPHPFGIHDLVMLDRVSDP
                     QISPDGKHVAYQVRETDYEANKGKNGIWAIELGNPKAQAMRLTDAAANATAPRWSADG
                     SAIYYQDKGQIWRLPAGGKGAALQATDLALDINAYKFSADGKQVLLSIDTWPDCAKEN
                     DPIACTKKRGDDKAKSKASGRVQDKLFVRHWDAWADGLRSQLFVATVGADGKIGAPKL
                     LTRGIDGDMPSKPFGGDDEFAFSSDGKTVYFSVRIAGTSEPWSTNFDIYSVPADASAV
                     PKNLTTANPAWDTAPVPSADGKTLYYLAMKRAGFEADRFGIMAMDLASGATREIDPQW
                     DRSAGGLKLSADAKTLFATADDNGEHPLFAVDIASGKVTKLAGQGSVHDYSVHGNEII
                     FSRATLKTPADLFRVAA"
     gene            complement(1358..2116)
                     /locus_tag="VFA35_10065"
     CDS             complement(1358..2116)
                     /locus_tag="VFA35_10065"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZT56562.1"
                     /translation="MNTPANANVSGNDKYPPNSTFTPVTPPSHGTVTLYPDGSYTYTP
                     ANNYSGTDTFTYKVCELAPNDTLCSTATVTVTVAAGVSDMMATGAATQTVPVNTTITV
                     VTTCTNNGPQVAINATCSVAGIPTTATFAAAPGAGVALPSAAAAAAGPGSGTTCVPVT
                     PVANLALGGVITCTTMFTPTQAGTVTLQTTVASDTPDSNPANDVAPSVVIVTDNAPPR
                     TLAAPVPAMSQWTLLLLGLLLAAAGAARSAAHRR"
     gene            2025..2219
                     /locus_tag="VFA35_10070"
     CDS             2025..2219
                     /locus_tag="VFA35_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56563.1"
                     /translation="MPWLGGVTGVNVEFGGYLSLPDTFAFAGVFTVVVNVSSFAFGPT
                     TIVTAVEPQRAGGPELSHTL"
     gene            2226..>2489
                     /locus_tag="VFA35_10075"
     CDS             2226..>2489
                     /locus_tag="VFA35_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56564.1"
                     /translation="MSTPVKPAFGVYAQLPEASQLTVPCAGCVGTTDAGLIVEPATVS
                     FAATLMVTGVLNGVVPESGVGVIWPGLTRVVAVLFDAIGSSVSL"
BASE COUNT          388 a          825 c          820 g          456 t
ORIGIN      
        1 cgcggcgacg cggaacaggt cggccggtgt cttcaacgtc gcacgcgaga aaatgatctc
       61 gttgccgtgc acgctgtagt cgtgcaccga gccctggcct gcgagtttcg tcaccttgcc
      121 cgaagcgata tcgaccgcga acagcggatg ctcgccattg tcgtccgcgg tcgcaaacag
      181 tgtcttggcg tcggccgaga gcttgagccc gccggccgaa cgatcccatt gcggatcgat
      241 ctcgcgcgtc gcaccgctcg ccaggtccat cgccatgatg ccgaaacggt ccgcctcgaa
      301 ccccgcgcgc ttcatcgcga ggtagtacag cgtcttgccg tctgcggacg gcaccggcgc
      361 ggtgtcccag gccggattcg ccgtggtcag attcttcggc acggcgctgg cgtcggccgg
      421 caccgagtag atgtcgaaat tcgtcgacca cggctcgctg gtaccggcga tgcgcacgct
      481 gaaatagacg gtcttgccgt cggacgagaa cgcgaactcg tcatcgccac cgaacggctt
      541 cgacggcatg tcgccatcga tgccgcgcgt cagcagtttc ggcgcgccga tttttccgtc
      601 cgcgccgacc gtagcgacga acagctgcga acgcagccca tcggcccacg catcccagtg
      661 gcgcacgaac agcttgtcct ggacgcgacc gctggccttg ctcttcgcct tgtcgtcgcc
      721 gcgcttcttc gtgcaggcga tcggatcgtt ttccttggcg cagtcgggcc aggtgtcgat
      781 ggacagcagc acctgcttgc cgtcggcgga gaatttgtac gcattgatgt cgagagcaag
      841 atcggtagcc tgcaatgcgg cgcccttgcc gccggcgggc aaacgccaga tctggccctt
      901 gtcctgatag tagatcgcgc tgccgtccgc cgaccagcgc ggcgcggtcg cattcgcagc
      961 cgcatcggtc aggcgcatgg cctgcgcctt cggattgccg agttcgatcg cccagatgcc
     1021 gttcttgccc ttgtttgcct cgtagtcggt ctcgcgcacc tgataggcga cgtgcttgcc
     1081 gtccggcgat atctgcggat cgctgacgcg atcaagcatg accaggtcgt ggatgccgaa
     1141 tggatgcggc acggacgcgg cggcgtgcgc gcagatggca atggctgcgc aggcgatcaa
     1201 cgacttcatc ggaattctcc caaggatcga gccggcatgg tacgcgagcg cgacaggcca
     1261 caaaagccgt gccgcgcaaa tcacgcgacg atgggagaat gcagtctcgc aaccgcagcc
     1321 gttcctgccc atcgaggcag aaacggccaa gtcaatgtca gcgacgatgc gctgcgctgc
     1381 gcgcggcacc cgctgcggca agcaacaggc cgagcagcaa cagtgtccat tgcgacatcg
     1441 ccggcaccgg cgccgcaagc gtccgcggcg gcgcgttgtc ggtcacgatc accacgctcg
     1501 gcgccacgtc gttcgccgga ttcgagtcgg gcgtgtcgct ggccacggtc gtctgcagcg
     1561 tgaccgtgcc cgcctgcgtc ggcgtgaaca tggtcgtgca ggtgatcacg ccgcccagcg
     1621 caaggttggc caccggcgtc accggcacgc aggtcgtgcc cgaaccgggt ccggccgcag
     1681 ccgcagcggc cgagggcaag gcgacgcctg ccccaggcgc agcggcgaag gtcgccgtgg
     1741 tcgggatgcc cgcgaccgag caggtcgcat tgatcgcgac ttgcgggccg ttgttcgtgc
     1801 acgtcgtcac caccgttatc gtcgtgttga cgggcacggt ctgcgtcgcc gcgccagtcg
     1861 ccatcatgtc ggagacgccc gccgccaccg tgaccgtcac ggtcgccgtg ctgcacagcg
     1921 tgtcattggg cgccagctcg cacaccttgt acgtgaacgt atccgtgccg ctgtagttgt
     1981 tcgccggcgt ataggtatag ctgccgtccg gatacaacgt caccgtgccg tggctcggcg
     2041 gcgtcaccgg cgtgaatgtc gaattcggcg gatacttgtc gttgcccgac acgtttgcgt
     2101 tcgccggcgt gttcaccgtc gtcgtcaacg tgtcgtcgtt cgccttcggt ccgaccacga
     2161 tcgtcaccgc cgtggagccg cagcgcgctg gcgggccgga gttgtcgcac acgctgtagg
     2221 tgtacgtgtc cacaccggtg aagcccgcat tcggtgtgta cgcgcagttg cccgaggcat
     2281 cgcagctcac tgtgccgtgc gccggctgcg tcggcaccac cgatgccgga ttgatcgtcg
     2341 agcccgcaac agtgtcgttc gcagccacgt tgatggtgac cggcgtgttg aacggggtcg
     2401 tgcccgaatc cggcgtcggc gtgatctggc ccggattgac cagggtcgtg gccgtgttgt
     2461 tcgacgcaat cggatcgagc gtgtcgctg
//
LOCUS       DASRDG010000603         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1126049, whole genome shotgun sequence.
ACCESSION   DASRDG010000603 DASRDG010000000
VERSION     DASRDG010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126049"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFA35_10080"
     CDS             complement(<1..569)
                     /locus_tag="VFA35_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZT56565.1"
                     /translation="MGATDALLLDAYSQTVSDVVERLGPSVAAVQVGDAGAAGAGGGS
                     GFLFTPDGYVLTNSHVVRAGKPGFAPGEPKPAYRVSLADARSYRARWVGDDPDTDLAV
                     LHIDGLSQGRLEHAPLGSSVGLKRGEIAIAIGNPLGFEHTVTSGIVSALGRSMRTSTG
                     RLIPNVIQTDAALNPGNSGGPLLNARGEV"
     gene            747..1331
                     /locus_tag="VFA35_10085"
     CDS             747..1331
                     /locus_tag="VFA35_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HZT56566.1"
                     /translation="MMSPAECAQQLKQLFPALFGGAVKPLKLRIQVDIQARAPGRFGK
                     QALSAFFRRHTGATSYLIAMTKAPHRFDLDGQAAGEITDEHRKVAADELARRRGNQEE
                     RRQLEEQQRRNRAGLLHDFQTTTLTKANFCALKGVPVDELDGLLEIARGEAEERARQA
                     PPPRHAPAFDRPRGTGGRPGPGGDRSTRGGRGRG"
     gene            complement(1357..1554)
                     /locus_tag="VFA35_10090"
     CDS             complement(1357..1554)
                     /locus_tag="VFA35_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56567.1"
                     /translation="MHLEQLMGRYFRLKQELALAYREQPWHGARIDRLADDLSATERE
                     IAALQPTDEQCGDPMLGFATP"
     gene            1809..2063
                     /locus_tag="VFA35_10095"
     CDS             1809..2063
                     /locus_tag="VFA35_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56568.1"
                     /translation="MKKLILALAIVLAGGASIAQTTEAVKESGKAVAEKAKEGKENVQ
                     AAATSDPKKKATHKAKAKAHKAKALKHDAKAKAAGEQIGK"
     gene            2098..>2725
                     /locus_tag="VFA35_10100"
     CDS             2098..>2725
                     /locus_tag="VFA35_10100"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZT56569.1"
                     /translation="MKLRKLALGLLAALVVAGVAGWFGLDKETRGLLATLPTDRDVLF
                     WTQPQRDAAFRALDRLPFLARSRPVPAGGTVLPLPPGPPLKLALDVDAYMAGQRSAAL
                     LVVHDGRLRLERYGLGFDAHGRWTSFSVAKSITSTLLGAALRDGDIRSLDDKVSEYIP
                     QMKGSAYDDVSIRQLLTMTSGVKWNEDYGDPRSDVALFNKHRPEEGVDA"
BASE COUNT          440 a          952 c          964 g          369 t
ORIGIN      
        1 atcacctcgc cgcgcgcgtt cagcagcggg ccgccggagt tgccggggtt gagcgcggca
       61 tcggtctgga tcacgttcgg gatcagccgg ccggtgctgg tgcgcatgct gcgcccgagc
      121 gcgctgacga tgcccgaggt caccgtgtgc tcgaagccga gcgggttgcc gatcgcgatc
      181 gcgatctcgc cgcgcttcag gccgaccgag ctgcccagcg gcgcgtgctc cagccggcct
      241 tgcgagaggc cgtcgatgtg cagcacggcg aggtcggtgt cggggtcgtc gccgacccag
      301 cgggcgcggt agctgcgcgc atcggcgagc gagacgcggt acgcgggctt gggctcgccc
      361 ggcgcgaagc ccggcttgcc ggcgcgcacc acgtgcgagt tggtcagcac atagccgtcg
      421 ggcgtgaaca gaaagcccga cccgccgccg gcgccggccg cgcccgcgtc gccgacctgc
      481 accgcggcca cgctcggccc caggcgctcg accacgtcgc tgacggtctg cgaatacgcg
      541 tcgagcagca aagcatccgt tgcacccatg agtcatctct cctgctggat tcggaagtag
      601 gggtgacggg cggatttgca agcacaatcc agcccttcca cgtcatccgc gctgccgctc
      661 ccgttcccat gtcagagacc tcgccgctgc ccatgccaga cgccgcaccg gacgccgcac
      721 cgacggttgc gccgagtccc gcgccgatga tgtccccggc cgaatgcgcg cagcagctca
      781 agcagctgtt tccggcgctg tttggcggcg cggtgaagcc cttgaagctg cgcatccagg
      841 tcgacatcca ggcgcgcgcg ccgggcaggt tcggcaagca ggcgctgtcg gcgttcttcc
      901 gccgccacac cggcgcgacc tcgtacctga tcgcgatgac gaaggcgccg catcgcttcg
      961 acctcgacgg ccaagccgca ggcgagatca ccgacgagca ccgcaaggtc gcggccgacg
     1021 aactcgcgcg ccgccgcggc aaccaggagg agcggcgcca gctcgaagag cagcagcgcc
     1081 gcaaccgcgc cgggttgctg cacgacttcc agacgacgac gctgacgaag gcgaatttct
     1141 gtgcgctgaa gggtgtgccg gtcgacgaac tcgacggctt gctcgagatc gcgcgcggcg
     1201 aggccgagga gcgcgcccgg caggcgccgc caccgcgcca tgcgccggcc ttcgacagac
     1261 cgcgcggcac cggcggccgg cccggcccgg gcggcgaccg ttcgacccgg ggcggccgag
     1321 gccgcggtta gtgcgaccgc cggcggggag ggcgggtcag ggcgtcgcga agccgagcat
     1381 cgggtcgccg cactgttcgt cggtgggttg cagcgcggcg atctcgcgtt cggtcgcgga
     1441 caggtcgtcg gccaggcgat cgatgcgcgc gccgtgccaa ggctgctcgc ggtaggccag
     1501 cgccagctcc tgcttcagcc ggaagtaccg gcccatcaac tgctcaaggt gcatcgtcgc
     1561 actcccatat ggtgcggtgc agtatgagcg agtcggtagg cggcgcgtgt aggacaaggt
     1621 cgcccgacga ccccctcatc gagggggtga ggccgaaaca atgtcacgac gttgccaact
     1681 gttcacgctg cgggtggcgg cacggcacgc tcgcgtgaac ctgtgcgcag aggcctatga
     1741 tcccgtgcct gtcgccgacg cgtggccgca ggccattcca ttcaactcaa caccgaggag
     1801 tcaccaacat gaagaaactg atcctggcgc tggccatcgt gctcgccggc ggcgcaagca
     1861 tcgcccagac cacggaagcc gtgaaggagt ccggcaaggc cgtcgcagag aaggcgaagg
     1921 agggcaagga gaacgttcag gccgcggcga ccagcgaccc gaagaagaag gcgacgcaca
     1981 aggccaaggc caaggcgcac aaggccaagg ccctgaagca cgacgcgaag gccaaggcgg
     2041 ccggcgagca gatcggcaag tagccgcttc cgggcgcgtg cacgctgccc gactgccatg
     2101 aagctcagga agctggcgct cgggctcctg gctgcgcttg tcgttgccgg cgtcgccgga
     2161 tggttcggtc tcgacaagga aacgcgcggg ctgctggcga cgctgccgac cgaccgcgac
     2221 gttctcttct ggacccagcc gcagcgcgac gccgcgtttc gcgcgctcga ccggctcccc
     2281 ttcctggccc ggtcgcggcc cgtgccagcc ggcggcaccg tgttgccgct gccgccgggg
     2341 ccgccgctga agctcgcgct cgacgtcgac gcctacatgg cgggccagcg cagcgcggcg
     2401 ctgctggtcg tgcacgacgg caggctgcgc ctggagcgct atggcctggg cttcgacgcc
     2461 cacgggcgct ggacgagctt ctcggtcgcc aagtcgatca cttcgacctt gctgggcgcg
     2521 gcgctgcgcg acggcgacat ccgcagcctg gacgacaagg tcagcgagta catcccgcag
     2581 atgaaaggct cggcctacga cgacgtcagc atccgccagc tgctgacgat gacctcgggc
     2641 gtgaagtgga acgaggacta cggcgacccc cgatccgacg tcgcgctgtt caacaagcac
     2701 cggcccgagg aaggcgtcga tgcgc
//
LOCUS       DASRDG010000604         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_801087, whole genome shotgun sequence.
ACCESSION   DASRDG010000604 DASRDG010000000
VERSION     DASRDG010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801087"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..2249
                     /locus_tag="VFA35_10105"
     CDS             90..2249
                     /locus_tag="VFA35_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZT56570.1"
                     /translation="MPPLNLEAIPSLRYAAWLRTLLGYRVLNGLSVGPGLAVMSVLVY
                     LAAGPAVAVSALLGMLIVSIGDQVAPARGKLRQLAPLLWMAAPMSAAVQFAHLVPDHS
                     TALIGAVVCVCGFIAMLGTAWGARGAPLSFALVLTIVFAMSTSPTDWRAALVRVAWFQ
                     VGAFLYAAWAVFSAWILNLRFRTQALAEAIDELAVMLRQQGKRLAATGDRRENELRAL
                     LGEQATLADKLQAARDLVLDLPASERELRLAGMLIAAIRLREQALACELELDMQPARG
                     CAPPAPAQVQALESLWGEFSADLDRVGWSLLGGGELPARLAESLQARTADAQRAVPPA
                     VRGAVDAMCAEIGSLLRLAERGGDEPRLALAEQARDWPRFRTSMRWPIAPLKRAMHGQ
                     SPVLRYALRVSIALLVGYIIGLHLPWATHPQWILLSIAVVMRTNLAQTLERRNARLLG
                     TLIGCVLVTALLSLNPSVPVQFLVLALGAGIAHGFVQVRYLVAATAATVLALIQGHLL
                     HTAGEFALFERLADTLIGTALAWVFSYVLPAWERRQLPALLKRLRNAQLQHAQVALGS
                     AELSTANADWRLARREVHDSLAAIALAAQRARSEPRAVQPPLDLLEKVQLRSYRLLAQ
                     LGGLRTWREQPRALPATEAAPLLAAHLARITAALAASSAATGTDAPIEARTETISGFE
                     ELAQLHRRLDDAADEARALGADLAAAQAWEAARLQEK"
     gene            2252..>4224
                     /gene="mutS"
                     /locus_tag="VFA35_10110"
     CDS             2252..>4224
                     /gene="mutS"
                     /locus_tag="VFA35_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZT56571.1"
                     /translation="MTSTRTPAAAPAPVPALSPMMAQYTKIKADFPDTLVFYRMGDFY
                     ELFFDDARKANRLLDITLTARGSSGGEPIPMAGVPVVSLDAYLGKLIKLGEAVAICEQ
                     VGDVATAKGPVERKVVRVVTPGTVTDTELLADKSDALLLAVSRQGRQIGLAWLALTSG
                     ELGMTECAEGQLGAWLARLAPAEVLVDRDHVPAALAGAPLTITHRPAWQFDTALGLRK
                     LCAQLRVASLAGFNAQDLRAAQAAGAALLSFAEHTQGQALAHVRTLEVQRASDLLDLP
                     PTTHRNLELTQTLRGDDAPTLLSLLDTCRTGMGSRALRHWLTHPGRERRAATQRHDAI
                     DALHTARVGELRDALRGVSDVERITARIALRQVRPRELAGLRATLRALPALREQVPLG
                     EATLLDMLGEALAPPAAILELLADAIADEPAVLLRDGGVIAPGFDAALDELRGIGQNC
                     DAYLIDLESRERARTGIANLRVQFNKVHGFYIEVTQGQAGKVPADYQRRQTLKNAERF
                     ITPELKGFEDKALSAQDRALAREKLLYEQLLDSLQAHIEPLSALARALASLDALAALA
                     ERATTLNWCRPEFVREPCIRIEAGRHPVVEARLLELGAGNFVANDCKLDAAQKMLVIT
                     GPNMGGKSTFMRQVALIALLAAMGSFVPARACRL"
BASE COUNT          564 a         1573 c         1504 g          583 t
ORIGIN      
        1 gacgccgagc gcaaggtgcc gcgcgcgcgc ccgccgcgtg gaggcgacac gccgttctga
       61 ggccggcatc cggtcgccgc agcatgcaga tgccgccgct gaacctcgaa gcgatcccct
      121 cgctgcgcta tgcggcgtgg ctgcgcacgc tgctcggcta tcgcgtgctg aacggcctgt
      181 cggtcggccc ggggctggcg gtgatgtcgg tgctggtcta cctcgccgcc gggccggcgg
      241 tggccgtgtc ggccttgctc ggcatgctga tcgtgagcat cggcgaccag gttgcgccgg
      301 cgcgcggcaa gctgcgccag ctcgcgccgc tgctgtggat ggccgcgccg atgtcggcgg
      361 cggtgcagtt cgcgcacctc gtgcccgatc actcgaccgc gctgatcggc gcggtcgtct
      421 gcgtctgcgg cttcatcgcc atgctcggca cggcgtgggg cgcgcgcggc gcgccgctca
      481 gcttcgcgct ggtgctcacg atcgtgttcg cgatgtcgac gtcgccgacc gactggcgcg
      541 ccgccctcgt gcgcgtcgcg tggttccagg tcggcgcgtt tctctacgcg gcctgggccg
      601 tgttcagcgc gtggatactg aacctgcgct ttcgcaccca ggcgctggcc gaagcgatcg
      661 acgaactcgc ggtgatgctg cgccagcagg gcaagcgcct ggccgcgacc ggcgaccggc
      721 gcgagaacga gttgcgcgcg ctgctcggcg agcaggcgac gctggccgac aagctgcagg
      781 ccgcgcgcga tctggtgctc gacctgccgg ccagcgagcg cgagctgcgc ctggccggca
      841 tgctgatcgc cgcgatccgc ctgcgcgaac aagccctcgc ctgcgagctc gaactcgaca
      901 tgcagccggc ccgtggctgc gccccgccgg cgccggcgca ggtgcaagcg ctcgagtcgc
      961 tgtggggcga gttcagcgcc gacctcgacc gtgtcggctg gtcgctgctc ggcggcggcg
     1021 aactgccggc gcggctggcc gagtcgctgc aggcgcgcac ggccgacgcg caacgcgcgg
     1081 tgccgcctgc cgtgcgcggc gccgtcgacg cgatgtgcgc cgagatcggc agcctgctgc
     1141 gcctggccga acgcggcggc gacgagcccc gcctggcgct ggccgagcag gcacgcgact
     1201 ggccgcgctt tcgcacctcg atgcgctggc cgatcgcgcc gctgaagcgc gcgatgcacg
     1261 gccagtcgcc ggtgctgcgc tatgcgctgc gggtatcgat cgcgctgctg gtcggctaca
     1321 tcatcggcct gcacctgccc tgggcgacgc acccgcaatg gatcctgctg tcgatcgcgg
     1381 tcgtgatgcg aaccaacctc gcgcagacgc tggagcgccg caacgcgcgc ctgctcggca
     1441 cgctgatcgg ctgcgtgctc gtcaccgcgc tgctcagcct gaacccgagc gtgccggtgc
     1501 agttcctcgt gctcgcgctc ggcgccggca tcgcgcacgg cttcgtgcag gtgcgctacc
     1561 tcgtcgccgc cacggccgcg accgtgctcg cgctgatcca gggccacctg ttgcacaccg
     1621 ccggcgagtt cgcgctgttc gagcggctgg ccgacacgct gatcggcacc gcgctcgcct
     1681 gggtcttcag ctacgtgctg ccggcctggg agcggcgcca gttgccggcc ctgctgaagc
     1741 gcctgcgcaa tgcgcagctg cagcacgcgc aggtcgcgct cggcagcgcc gagctctcga
     1801 ccgcgaacgc cgactggcgc ctcgcgcgcc gcgaggtgca cgacagcctc gccgcgatcg
     1861 cgctggcggc gcaacgcgcg cgcagcgaac cgcgcgcggt gcagccgccg ctcgatctgc
     1921 tcgagaaggt gcagctgcgc agctaccgtc tgctcgcgca gctcggcggc ctgcgcacct
     1981 ggcgcgaaca accgcgcgca ttgcccgcca ccgaggccgc gccgctgctc gcggcgcacc
     2041 tggcgcgaat caccgcggcg ctggcggctt cgtcggccgc gaccggcacc gatgcgccga
     2101 tcgaggcgcg taccgaaacc atctccggct tcgaggagct cgcgcaactg caccggcgcc
     2161 tggacgacgc ggccgacgag gcccgcgcac tcggcgccga cctggccgca gcccaggcct
     2221 gggaagcagc gcgcctgcag gaaaagtagc gatgacatcg acccgcacgc ccgccgcggc
     2281 accggcacca gtaccggcac tctcgccgat gatggcccag tacaccaaga tcaaggccga
     2341 ttttcccgac acgctcgtgt tctatcgcat gggcgatttc tacgagctgt tcttcgacga
     2401 cgcgcgcaag gcgaaccgcc tgctcgacat caccttgacc gcgcgcggca gcagcggcgg
     2461 cgagccgatc ccgatggctg gcgtgccggt ggtttcgctc gacgcctatc tgggcaagct
     2521 gatcaagctg ggcgaagcgg tcgcgatctg cgagcaggtc ggcgacgtgg cgacggcgaa
     2581 ggggccggtc gagcgcaagg tcgtgcgcgt ggtcacgccg ggcacggtaa ccgacaccga
     2641 actgctcgcc gacaagagcg acgcgctgct gctcgccgtc tcccgccaag gccgacaaat
     2701 cggcctggcg tggctcgcgc tgaccagcgg cgagctcggg atgaccgagt gcgccgaagg
     2761 ccagctgggc gcctggctcg cgcgcctggc gccggccgaa gtgctggtcg atcgcgatca
     2821 cgtgccggcg gcgcttgcgg gcgccccgct cacgatcacg caccgcccgg cctggcagtt
     2881 cgacaccgcg ctgggcctgc gcaagctctg cgcgcagttg cgcgtcgcga gcctggccgg
     2941 cttcaacgcg caagacctgc gcgccgcgca ggctgccggc gcggcgctgc tgagcttcgc
     3001 cgagcacacg cagggccagg cgctcgcgca cgtgcgcacg ctcgaagtcc agcgtgcgag
     3061 cgacctgctc gacctgccac cgacgacgca tcgcaacctc gaactgacgc agaccctgcg
     3121 cggcgacgac gcgccgacgc tgctctcgct gctcgacacc tgccgcaccg gcatgggctc
     3181 gcgcgcgctg cgccactggt tgacgcaccc ggggcgcgaa cggcgtgcgg caacccagcg
     3241 gcacgacgcg atcgatgcgc tgcacaccgc gcgcgtgggc gagttgcgcg acgcgctgcg
     3301 cggcgtcagc gatgtcgagc gcatcaccgc gcgcatcgcg ctgcgccagg tgcgcccgcg
     3361 cgagctcgcc ggcctgcgcg cgacactgcg cgcgctgccc gcgctgcgcg agcaggtgcc
     3421 gctgggcgaa gcgacgctgc tcgacatgct cggcgaggcg ctcgcgccgc cggccgcgat
     3481 cctcgagctc ctggccgacg ccatcgccga cgagcccgcc gtgctgctgc gcgacggcgg
     3541 cgtcatcgcc cccggcttcg acgccgcgct cgacgagctg cgcggcatcg gccagaactg
     3601 tgacgcgtac ctcatcgacc tcgagtcgcg cgaacgcgcg cgcaccggca tcgcgaacct
     3661 gcgggtgcag ttcaacaagg tgcacggctt ctacatcgag gtcacgcagg gccaggcggg
     3721 caaggtgccg gccgactacc agcgccgcca gacgctgaag aacgccgagc gcttcatcac
     3781 accggagctg aagggcttcg aggacaaggc gctctcggcg caggaccgcg cgctggcgcg
     3841 cgagaagctg ctgtacgagc agctgctcga cagcctccag gcgcacatcg agccgctctc
     3901 ggcgctggcc cgcgcgctcg cgagcctcga tgcgctcgcg gcattggccg agcgcgcgac
     3961 gacgctgaac tggtgccgcc cggagttcgt ccgcgaaccc tgcatccgca tcgaggccgg
     4021 ccggcacccg gtcgtcgagg cgcggctgct ggaactgggt gccggcaact tcgtggccaa
     4081 cgactgcaag ctcgacgccg cgcagaagat gctcgtgatc accggcccga acatgggcgg
     4141 caagagcacc ttcatgcgcc aggtcgcgct gatcgcgctg ctcgccgcga tgggctcgtt
     4201 cgtgcccgcg cgggcgtgtc gcct
//
LOCUS       DASRDG010000605         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1245571, whole genome shotgun sequence.
ACCESSION   DASRDG010000605 DASRDG010000000
VERSION     DASRDG010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245571"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFA35_10115"
     CDS             complement(<1..411)
                     /locus_tag="VFA35_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZT56572.1"
                     /translation="MNLLALPAFTDNYIWMLHDGRQALVVDPGESAPVIAALDARHLV
                     LAAILVTHHHADHVGGVDALRTRLHGPVYGPRRESIPQPYRPLAGGDAIEVLGLRFEV
                     IEVPGHTSGHIAYFHARDAADPDAAPILFCGDTLF"
     gene            410..1249
                     /locus_tag="VFA35_10120"
     CDS             410..1249
                     /locus_tag="VFA35_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZT56573.1"
                     /translation="MAEQAIIGLTDWLKTPAGAYVLQWEQAHLDTAVVDLFGFHALQL
                     GLPELEALRANRMPHRWVATEQASDPPGVDRDALPGEAPPPRIAIALRCDFDALPFDT
                     NSLDLVVLPHALELARDPHLALREVERVLVPEGRVVIVGFNPTSLWGLRQHLGRWRRR
                     LLPGSPRGLFLPSAGEFLGYRRLRDWLRLLSFEVEDGRFGCWRPPVASMRWLARFAWA
                     DRVGERWWPVFGAVYFVVAVKRVRGMRLVGMVRAPRAVPQAAPAPVVATTHHRHQLEE
                     QDS"
     gene            1246..1731
                     /gene="rnhA"
                     /locus_tag="VFA35_10125"
     CDS             1246..1731
                     /gene="rnhA"
                     /locus_tag="VFA35_10125"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZT56574.1"
                     /translation="MSIEATQDAAPKPPAIQRPHVVIYTDGACKGNPGRGGWGAWLRT
                     GEHQKELFGGEPLTTNNRMELTAVIEALNSLKRTCDVTVHTDSEYVRKGITEWIHGWK
                     LRGWKTADKKPVKNAELWQRLDALRNLHHVEWRWVKGHSGDPGNERADALANKGVESV
                     R"
     gene            complement(1753..2382)
                     /locus_tag="VFA35_10130"
     CDS             complement(1753..2382)
                     /locus_tag="VFA35_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZT56575.1"
                     /translation="MLLTPDPSDDLYRFALEFGYSAHLTLSRDLYGSEFSLVALHASC
                     PAPAHASMYSALFGCPVHFGHRSDEVVIDLAWSAVAAGMPDPVTHEAVREICQQFEVD
                     LAHSGGTASIVRRTLVEQMPWRFPTIDAMATALEMHPRTLRRRLESDGTSFKDLLSEV
                     RRRLAIEYLRRTRMTTEEIASRLGYSDAANFRHAFARWTGRTPHEYRTA"
BASE COUNT          388 a          805 c          869 g          367 t
ORIGIN      
        1 gaacagcgtg tcgccgcaga acagaatcgg cgcggcgtcg gggtcggcgg cgtcgcgtgc
       61 gtggaaatac gcgatgtgcc cgctcgtgtg gccgggcacc tcgatgacct cgaagcgcag
      121 cccgagcacc tcgatcgcgt cgccaccggc gagcggccgg tacggctgcg ggatggactc
      181 gcgccgcggg ccgtacacgg gcccgtgcag gcgtgtgcgc aaggcgtcga cgccaccgac
      241 atgatcggcg tggtggtggg tcactagaat tgccgccagc accaggtgcc gtgcgtcgag
      301 cgcggcgatc actggcgcgg actcacccgg atcgacaacg agggcttgtc ggccatcgtg
      361 gagcatccag atgtagttgt cggtgaaggc gggcagggcg agcaggttca tggcggaaca
      421 ggcgattata ggtttgaccg actggttgaa gacccctgca ggtgcttacg ttctgcaatg
      481 ggaacaggcc catttggaca ccgccgtcgt cgacctgttc ggcttccacg cgctgcagct
      541 cggcctgccc gaactcgagg ccttgcgggc caaccggatg ccgcatcgct gggtcgcgac
      601 cgagcaggcg tcggatccgc ccggagtgga ccgggatgct ttacccggtg aagcaccgcc
      661 gccgcgcatc gcgatcgcgc tgcggtgcga cttcgatgcc ttgcccttcg acaccaacag
      721 tctcgacctg gtcgtgctgc cgcatgcgct cgagctcgca cgcgacccgc acctcgcgct
      781 gcgcgaggtc gagcgcgtgc tcgtgcccga aggccgggtc gtgatcgtcg gcttcaaccc
      841 gaccagcctg tggggcctgc gccagcacct cgggcgctgg cgccggcgcc tgctgccggg
      901 ctcgccgcgc gggctgttcc tgccgagcgc gggcgagttc ctgggctacc ggcggctgcg
      961 cgactggctg cggctgctga gcttcgaggt cgaggacggg cgcttcggct gctggcgccc
     1021 gccggtcgcg tcgatgcgct ggctggcgcg cttcgcctgg gccgatcgcg tcggcgagcg
     1081 ctggtggccg gtgttcggcg cggtctactt cgtcgtcgcg gtcaagcgcg tgcgcggcat
     1141 gcggctggtc ggcatggtgc gcgcgccgcg cgccgtgccg caggccgcgc cggcgccggt
     1201 cgtcgcgacg acccatcatc ggcatcaact cgaagaacag gattcatgag catcgaagca
     1261 acccaagacg cggcacccaa gccgccggcg atccagcgcc cgcatgtcgt catctacacc
     1321 gacggcgcct gcaagggcaa ccccggccgc ggcggctggg gcgcgtggct gcgcaccggc
     1381 gagcaccaga aggaactgtt cggcggcgag ccgctgacca ccaacaaccg catggaactc
     1441 accgcggtga tcgaggcgct gaactcgttg aagcgcacct gcgacgtgac cgtgcacacc
     1501 gacagcgaat acgtgcgcaa ggggatcacc gaatggatcc acggctggaa gctgcgtggc
     1561 tggaagaccg ccgacaagaa gccggtgaag aacgccgaac tctggcagcg cctggacgcg
     1621 ctgcgcaacc tgcaccatgt cgagtggcgc tgggtcaagg ggcactcggg cgacccgggc
     1681 aacgagcgcg ccgatgcgct cgcgaacaag ggcgtcgagt cggtacgctg acgagcgccc
     1741 tgtctcgaca ggtcaggccg tgcggtactc gtgcggcgtt ctgccggtcc agcgcgcgaa
     1801 cgcgtggcgg aagttcgccg cgtcgctgta gcccaggcgg ctggcgatct cctcggtggt
     1861 catccgggtc cggcgcaggt actcgatcgc cagcctgcgg cgcacttcgg acaacaggtc
     1921 cttgaaactc gtgccgtcgc tctccagccg gcgacgcagc gtgcgtgggt gcatctcgag
     1981 cgccgtcgcc atggcatcga tggtcggaaa ccgccaaggc atctgttcca cgagcgtgcg
     2041 ccgcacgatc gaggcggtac cgcccgagtg cgccaggtcg acctcgaact gctggcagat
     2101 ctcgcgcacg gcctcgtgcg tgaccggatc gggcatcccg gcggcgaccg cggaccaggc
     2161 taggtcgatc acgacctcgt cactgcggtg accgaagtga acggggcagc cgaacagcgc
     2221 cgaatacatg ctggcgtgag caggcgcggg gcacgaggcg tgcagcgcga ccaagctgaa
     2281 ttcactgccg tacaggtctc gactgagcgt caggtgcgcc gagtagccaa attcgagcgc
     2341 aaagcggtat agatcgtcgc tggggtcggg cgtgagcagc acctcgtagg tgtagatccc
     2401 gacctctccc tcgcgcgaga aagcgatgg
//
LOCUS       DASRDG010000606         5656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_453633, whole genome shotgun sequence.
ACCESSION   DASRDG010000606 DASRDG010000000
VERSION     DASRDG010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453633"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFA35_10135"
     CDS             <1..968
                     /locus_tag="VFA35_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56576.1"
                     /translation="PYDGGNAEVAAIYINRMDLNLVRRMYATKTATDNIAFVVCNHPG
                     PDGSSQREVDDVLDTGLADQKRVACVAMEWSTSPGVNGGKPFTKFLTFGPDGSLLPSV
                     NLDGRGEKYMPGACVACHGGTQYNGQFAARGIAPSPYLGAGFLPFDTGNYLFGSGSGL
                     SEAAQSQAIHDLNMLVKATDNRGYPATTGLIDGWYTGTATTLNKQYVPAAWTALEASK
                     PGAAKFYREVVGGVCRTCHIALGSAQFDWDVKTGTFSGLPGSLANAHFCGGTADVAIN
                     ASMPNALISRDRLADRIGADPELAALMQQFLGCVTPAADPAYAKR"
     gene            1016..2314
                     /locus_tag="VFA35_10140"
     CDS             1016..2314
                     /locus_tag="VFA35_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZT56577.1"
                     /translation="MNLGRQRPSSAFALMTLVAVVGCAGPTSPSNPPAPAPAATVAAA
                     RPAAIAAAPAPAAAPAPNTFVAPAPIKFEDAIARAGQQLFAQAAPQLGGDSRVLVIDP
                     LIDANTGAQTVSTVKMGEQLESIAKSRNTRWAVKPLTREALAQKPLLLIGTLTPVTVE
                     RSVDTVPDAFRVWLTLIDLRSGKVVAKQLDRATVDSVNPEPLKYYADSPTWHKDRTVL
                     GYINSCQINTKIGDPADPDYLARLPAAAVVNEAILAYDGGKVPLANKLYKEAEPIADP
                     GDLRVLNGLYITSWQLGQRDAARDAFGKLVESGLEQKRLPVKLLFQPGKTQFNTIGDL
                     PQQYTMWIASLAQKAGNTPSCVRVVGHTSRTGSARGNETLSRQRAEAVQRMLERDNRA
                     LAAKLSAAGVGSREALVGLGTDDVRDALDRRVEFRVVDCV"
     gene            2330..>5656
                     /locus_tag="VFA35_10145"
     CDS             2330..>5656
                     /locus_tag="VFA35_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZT56578.1"
                     /translation="MTRLPRRRLPGWCLAFVIMLGGIVWPGTSHADQWTGTTWGPSCF
                     SCHGTGTFEANTAGLSNLKTYSAFSSHVSAGFTNSPTMMTFKSAVANAVANPSTPTGL
                     TDRDATELIRLYLLTALSSPVTAPTANAGPNQTVVRGRAVSLAGSGGSTYRWQLDSRP
                     AGSSAALSSTTSATAGLTPDVVGTYNVSLIVTSATGVDSAPDRIVVTATANAAPTANA
                     GANQTVTQGATVTLDGSASSDPDSGTTLTYTWSAPGGITLSNVNAQKPSFTANATGSF
                     TFGLVVRDEVQPSSSAFVTVTVNPAVGVLQPAPSPLTGFSARAGGSVADVKLLTLTNG
                     GPALSGVALNGFAFSSGYSQDTTQACTPAGSCCNASTTLMGGQSCQLWVKFLPPVAGN
                     YSAGNVSIGYVASGSASTSISVPLSGTATQSTIGTTLPGGPPLTQSFGSVQNGFSAIQ
                     TLTITNTGDASLNFSSDPKDPARLTGSAEYSIVGGTCAQGTPVAPTSGSCTLQLCFAP
                     LVANAALPNPRQATLTLASDAGNGPLVVTLTGTASALPVPSAVPNSLTFAATAWGDTS
                     ASQDVVLANPRSDVITYSLGTPSSADFVVVDDGSAACAARSIPANGGQCTVKLAFRPQ
                     VTGANCSGLRCADLSVSLTPTSGPTMSPLSVHAVGTAIPQLRTSANALTLTTMATVPI
                     TGSVVVTNQSNASVTLGSISFSGATSPYSLDGASTCMSSGTLAASASCVLSVRYSPPV
                     AASDEAVATVPFGSAGGSLTVDLHGTATALPQGIVQLGASTLAFGDTPLTTTARLDLP
                     VHNAGNKALTFSAFDVAGANAADFRVGGTCSTSTLLGIGADCTLSVDFVPGALGARTG
                     SLTVRSDGVDPALVVSLNGNGTPAPAPRVVFSPLDAATGAVSFGNQTVGGIYPARVVQ
                     LKNAGTAALQISGFTVDGSGFSLASTTCAGQLAPQTSCDLLVLFTPSAADTDYAGTLK
                     AATNAPGSPQLAPLRGRGVAYTAPVLIWQPAVTGLIFGTVSVGSLSTAQSVVLSNQGP
                     GGATLALLNAVGMAAAEFVVGPAQQPPDAFACIAGSVLQQGQSCRVDVRFVPAASGDR
                     SASLQVVSTGAAPTTLTLAGT"
BASE COUNT          951 a         1962 c         1842 g          901 t
ORIGIN      
        1 aaccgtacga cggtggcaat gccgaggtcg cggcgatcta catcaaccgc atggacttga
       61 acctggtgcg ccgcatgtac gccacgaaga cggcgaccga caacatcgcg tttgtcgtct
      121 gcaaccaccc ggggccggat ggctcgtcgc agcgcgaagt cgacgacgtg ctcgacaccg
      181 ggctcgccga ccagaagcga gtggcctgcg tcgcgatgga atggtcgacc tcgcccggcg
      241 tcaacggagg caagccgttc acgaaattcc tcaccttcgg cccggacggt tcgctgctgc
      301 cctcggtcaa tctcgatggc cgcggcgaga agtacatgcc cggcgcttgc gtggcctgcc
      361 atggcggcac gcaatacaac gggcagttcg cggcgcgcgg catcgcgccg tcgccctacc
      421 tcggcgccgg ctttctgccg ttcgataccg gcaactacct gttcggttcg ggttcgggcc
      481 tgtccgaggc tgcgcaaagt caggcgatcc acgatctgaa catgctggtc aaggcaaccg
      541 acaaccgcgg ctatccggcg accacggggc tgatcgacgg ctggtacacc ggcactgcca
      601 ccacgctgaa caagcagtac gtcccggccg cgtggacggc gctcgaagcg agcaagcccg
      661 gcgcggcgaa gttctaccgt gaggtcgtgg gcggcgtgtg ccgcacctgc cacatcgcgc
      721 tcggctcggc gcagttcgac tgggacgtca agaccggaac attcagcgga ctgcccggct
      781 cgctcgcgaa cgcgcacttc tgcggcggca ccgccgacgt ggcgatcaac gcgtcaatgc
      841 cgaatgctct gatttcgcgc gaccgcctcg ccgaccgcat cggcgccgat ccggagctgg
      901 cggcactcat gcagcagttc ctgggctgcg tgaccccggc ggccgacccg gcctacgcaa
      961 aacgatgatc cgctcgagcg gccctgtccc gaacggagct gaagggatgt tcaacatgaa
     1021 cctaggtcgt cagcgtccgt cgagcgcctt cgcgctcatg acactcgtcg cggtggtcgg
     1081 ctgcgccgga cccacctcgc catccaatcc gccggccccg gcgcccgcgg caacggttgc
     1141 cgccgcgcgg cccgcagcga tcgccgccgc gcccgcacct gccgccgcac cggccccgaa
     1201 caccttcgtc gcgccggcac cgatcaagtt tgaagacgcc atcgcacgcg ccggccagca
     1261 gctattcgcg caagcggccc cgcagctcgg cggcgattcg cgcgtactcg tgatcgaccc
     1321 gctgatcgac gcgaacaccg gcgcgcagac cgtcagcacc gtgaagatgg gcgaacagct
     1381 cgaatcgatc gcgaagagcc gcaacacgcg ttgggccgtg aagccgctga cgcgcgaagc
     1441 gctcgcgcaa aaaccgctgc tgctgatcgg cacgctgacg ccggtgaccg tcgaacgttc
     1501 ggtcgacacg gtgcccgatg cctttcgcgt ctggctaacc ttgatcgatc tgcgcagcgg
     1561 caaggtcgtc gcgaagcaac tcgaccgcgc gacggtcgac tcggtgaacc cggagccgct
     1621 gaagtactac gccgacagcc cgacctggca caaggacagg acggtgctgg gctacatcaa
     1681 ctcgtgccag atcaacacca agatcggcga cccggccgat cccgactacc tggcgcggct
     1741 gccggccgcc gcggtcgtca acgaagcgat cctcgcctac gacggcggca aggtgccgct
     1801 ggcgaacaag ctctacaagg aggccgagcc gatcgccgac ccgggcgacc tgcgcgtgct
     1861 gaacggcctg tacatcacga gctggcagct gggccagcgc gacgccgcgc gcgacgcgtt
     1921 cggcaagctc gtcgaatccg ggctcgagca gaagcgcctg ccggtgaagc tgctgttcca
     1981 gcctggcaag acgcagttca acacgatcgg cgacctgccg cagcagtaca cgatgtggat
     2041 cgcgtcgctc gcgcagaagg ccggcaacac gccatcgtgc gtgcgcgtgg tcggccacac
     2101 cagccgcacc ggcagcgcgc gcggcaacga aacgctgtcg cgccagcgcg ccgaagccgt
     2161 gcagcgcatg ctcgaacgcg acaaccgcgc gctcgccgcg aagctgagtg ccgctggcgt
     2221 cggctcgcgc gaggcgctgg tggggttggg cacggacgat gtgcgcgatg cactggatcg
     2281 gcgcgtggag ttcagggtag tggattgcgt ctagagaggc cgagcgatca tgacccgtct
     2341 tccgcgccgt cggctcccag gttggtgcct ggccttcgtc atcatgctgg gtggcatcgt
     2401 ctggccgggt acgtcccacg ccgaccagtg gacagggacc acctggggtc catcctgctt
     2461 cagctgccac ggcaccggga cgttcgaggc caatacggcg ggcctcagca atctcaagac
     2521 ctacagcgcg ttctcttccc atgtgtccgc gggattcacc aattccccga cgatgatgac
     2581 attcaagagc gccgtggcaa acgctgtggc gaatccctcc actcccaccg gtctgaccga
     2641 ccgcgatgcc accgagttga tccggctgta cctgctgacg gcgctctcgt ctcccgtgac
     2701 cgcaccaacc gccaatgccg gcccgaatca gacggtagtc cgcgggcgtg cagtgtcgct
     2761 ggcaggaagc ggcgggagca cgtaccgctg gcagctcgac agccggcctg caggaagctc
     2821 tgccgcattg agctccacga cgtcggcaac ggctggcctc acaccggacg tcgtggggac
     2881 ctataacgtc agcctcatcg tcaccagtgc gaccggcgtc gacagtgcgc cggaccgcat
     2941 cgtggtgacg gccaccgcga acgccgcgcc gacagcgaac gctggggcga atcaaacggt
     3001 gactcagggt gccaccgtca cgctcgacgg cagcgccagc agcgaccccg attcgggcac
     3061 gacgcttact tacacatgga gcgcgcccgg cggcatcacg ctgtcgaacg tgaacgcgca
     3121 gaagccgagt ttcacggcca acgcgacggg ctcgttcacg ttcgggttgg tcgttaggga
     3181 cgaagtccaa cccagcagca gcgcttttgt caccgtaacg gtaaacccgg cggtgggtgt
     3241 gttgcagccc gcgccgtcgc cgctgaccgg cttcagcgca cgtgccggcg gcagcgtggc
     3301 cgacgtcaag ctgctgacgc tcaccaacgg cggtcccgca ctgagcggcg tcgcgctgaa
     3361 cgggttcgcg ttctcgtccg gctactcgca ggacacgacc caggcctgta cgccggccgg
     3421 atcgtgctgc aatgccagca ccaccctgat gggcggccag agctgccaat tgtgggtcaa
     3481 gttcctgccg cctgttgccg gcaactacag cgccggcaac gtctcgatcg gctatgtcgc
     3541 gtcaggctcg gccagcacgt cgataagcgt cccgctcagc ggcaccgcca cgcagtccac
     3601 gatcggcacg acgttgccgg gcggcccgcc cctgactcaa tcgttcggtt cggtgcagaa
     3661 cgggttcagt gcgatccaga cgctgacgat caccaatacc ggcgatgcat cgctcaactt
     3721 cagttccgat ccgaaggacc cggcgcgcct gacgggcagc gcggaataca gcatcgtcgg
     3781 cggtacgtgc gcgcaaggca cgccggtggc gccgaccagc ggctcctgca cactccagtt
     3841 gtgcttcgcg ccgctggtcg cgaacgcggc gttgccgaac ccccgccagg ccaccttgac
     3901 attggcctcc gacgccggga acgggccgct ggtggtgacc ttgaccggta ccgccagcgc
     3961 actgcccgtg cctagcgcgg tgccgaattc gctgacgttt gcggcaacag cgtggggcga
     4021 caccagtgca agccaggatg tcgtcctcgc gaacccgcgc tcggacgtca tcacttactc
     4081 gctcggcacg ccgagcagcg ccgactttgt cgtcgttgac gacggcagcg cggcttgcgc
     4141 ggcgagaagc attccggcaa acggtggaca atgcacggtc aaactcgcgt tccgcccgca
     4201 ggtgaccggc gcaaactgct ccggactgcg ctgcgccgac ctgtcggtga gcttgacgcc
     4261 gacatcggga ccgacgatgt cgccgctgag cgtgcatgct gtgggaaccg ccatcccgca
     4321 gttgcgcaca tcggcgaacg cgctgaccct cacgacgatg gcgacggtac cgatcacagg
     4381 cagcgtcgtc gtcacgaacc agagcaacgc ctctgtcacg ctcggcagca tctcgttcag
     4441 tggagcgacc tcgccgtatt cgcttgatgg cgccagcacc tgcatgtcaa gtggcacact
     4501 ggctgcgagc gccagttgcg ttctgtctgt tcgctacagc ccgcccgtgg cggccagcga
     4561 cgaagctgtc gctaccgtgc cgttcggttc agccggcggc tcgctgactg tcgatctgca
     4621 cggcaccgcc acggcgcttc cgcaaggaat cgtgcaactt ggtgcctcga cgctcgcgtt
     4681 cggcgacacg ccgctaacga ccaccgctcg gctggatctg cctgtgcaca atgccggcaa
     4741 caaggccttg acgttttcgg cattcgacgt ggccggcgcc aacgccgccg acttccgtgt
     4801 cggcggtaca tgcagcacca gcacactgct cggcatcggt gccgactgca cgctttcggt
     4861 ggatttcgtt cccggcgcgc tgggcgcgcg caccggctca ctgacggttc gctccgacgg
     4921 tgtcgatccg gcactggtgg tctcgctcaa tggcaatggc acgccggctc cggcgccgcg
     4981 ggtggtgttc tcgccgctgg acgctgcgac cggcgcggtg tcgttcggca accagacggt
     5041 aggcgggatc tatccggcgc gggtcgtgca gttgaagaat gcaggcacgg cggcactgca
     5101 gatatcgggc ttcacggtcg atgggtcggg attctcgctc gcgagcacga cctgcgcggg
     5161 tcagttggca ccgcagacga gttgtgatct gctcgttctg ttcacgcctt cggcagccga
     5221 cacggattac gcgggaacgc tcaaagcagc gaccaatgcc ccaggctctc cgcaactggc
     5281 gccgctgcgc ggccggggtg tcgcctacac cgcgccggtg ctgatctggc agccggcggt
     5341 gaccgggttg atcttcggca cggtatcggt cggcagtcta tcgaccgcgc agtcggtcgt
     5401 gctgagcaat caggggccgg gcggcgcaac cttggccctg ctgaatgcgg tgggcatggc
     5461 ggcggccgag tttgtcgtcg ggccagctca acagcccccg gatgccttcg cctgcatcgc
     5521 cggctcggtg ttgcagcaag ggcagagttg ccgtgtcgat gtgcgcttcg tgcctgctgc
     5581 cagtggcgat cgaagtgcct cgctgcaagt cgtctcgacc ggcgcggcgc cgaccacgct
     5641 gacactcgcc ggaacc
//
LOCUS       DASRDG010000607         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1354118, whole genome shotgun sequence.
ACCESSION   DASRDG010000607 DASRDG010000000
VERSION     DASRDG010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354118"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFA35_10150"
     CDS             complement(<1..233)
                     /locus_tag="VFA35_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HZT56579.1"
                     /translation="MSGFASALAAPRPLLHAARFEANPATRETPWVKWTILGLGLAFF
                     ALFLLMPLAAVFFEAFRKGWATYVAALVEPDALS"
     gene            complement(230..1123)
                     /gene="cysT"
                     /locus_tag="VFA35_10155"
     CDS             complement(230..1123)
                     /gene="cysT"
                     /locus_tag="VFA35_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HZT56580.1"
                     /translation="MSAVLAPFPAPSAGALPKRKPRRVLPGFGLSLGYTIAYLSLLVL
                     IPLSAVFFKTATLGWEPFWNVVTAPRVVASYQLSFGASLLAAAINSVFGLLLAWSLVR
                     YSFPGKRLVDALIDLPFALPTAVAGIALTALYAGNGWLGGLLEPLGIKVAFKPLGVLV
                     ALVFIGLPFVVRTVQPVLEDIDTELEEAAASLGAHRWQTFRHVVFPVLTPALLTGFAL
                     AFARAVGEYGSVIFIAGNLPMVSEITPLMIITKLEQYDYAGATAIATVMLVFSFTLLL
                     AINGLQAWSAKTRGVGAGRRP"
     gene            complement(1123..2130)
                     /locus_tag="VFA35_10160"
     CDS             complement(1123..2130)
                     /locus_tag="VFA35_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZT56581.1"
                     /translation="MSKKLFLPVLFASLALAGAARAQAPAETLLNVSYDVSRELYKEV
                     NPAFVAQWKAATGKTLAINQSHGGSTAQAQAVANGLEADVVTMNQSTDIDVLAERGLV
                     APDWKKRFPFGAAPYTTTSVFLVRKGNPKKILDWADLARPGLAVIIPNPKTSGNGRYT
                     YLGAWGSVIARGGNEAQAKEFVAKLLANVPVLDGGGRGATTTFTQRQIGDVLVTFENE
                     AALIAREVGRDQFDIVYPTLTIEAEAPVAVNEKVTAKRGTRAAAQAYLDFLFTPVGQQ
                     IIARHDFRPRDPAVLKANAERFPPVKTFTVDSLLGGWGAVFKKHFGDGGLYDQLVLRR
                     S"
     gene            2292..2645
                     /locus_tag="VFA35_10165"
     CDS             2292..2645
                     /locus_tag="VFA35_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZT56582.1"
                     /translation="MTTNDALRVVCLCAGWCGVCRDYRATFDAAAASFGARAAFAWFD
                     IEDDAALLDDVDVENFPTVLIAHGDHLLFFGTITPQPATLARLVQRALAGELRPQAAA
                     PEVDAFVQRARAAGA"
     gene            complement(2656..2889)
                     /locus_tag="VFA35_10170"
     CDS             complement(2656..2889)
                     /locus_tag="VFA35_10170"
                     /inference="COORDINATES: protein motif:HMM:NF028296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CzcE family metal-binding protein"
                     /protein_id="HZT56583.1"
                     /translation="MLAARDPGSDPFGSAAGVAMVTRTIVIRPDTGYVNVTGGEVIRF
                     EVGAKSFVWNFNGTRSSFDLAAIAPNPRYPKRG"
     gene            complement(2987..3448)
                     /locus_tag="VFA35_10175"
                     /pseudo
     CDS             complement(2987..3448)
                     /locus_tag="VFA35_10175"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
     gene            3438..>3604
                     /locus_tag="VFA35_10180"
     CDS             3438..>3604
                     /locus_tag="VFA35_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HZT56584.1"
                     /translation="MAYMLLILEPRGQRAERGLEAGKQVYAQMLGFAEALKQRGVLQA
                     VESLATDATATR"
BASE COUNT          561 a         1243 c         1306 g          494 t
ORIGIN      
        1 gacagcgcgt cgggctcgac cagcgcggcg acgtaggtgg cccagccctt cctgaacgcc
       61 tcgaagaaga cggccgccag cggcatcagc aggaacaggg cgaagaacgc cagcccgaga
      121 ccgaggatgg tccacttgac ccacggcgtc tcgcgcgtcg ccgggttggc ctcgaagcgc
      181 gccgcgtgca gcagcgggcg cggcgcggcg agtgcggagg cgaagccgct catggcctgc
      241 gccccgcgcc gacgccgcgc gtcttcgcgc tccaggcctg cagcccgttg atcgcgagca
      301 gcagcgtgaa cgagaacacc agcatcacgg tggcgatcgc ggtcgcgccg gcgtagtcgt
      361 actgctcgag cttggtgatg atcatcagcg gcgtgatctc cgagaccatc ggcaggttgc
      421 cggcgatgaa gatcaccgag ccgtactcgc cgaccgcgcg cgcgaacgcg agcgcgaagc
      481 cggtcagcag cgccggcgtc agcaccggga acacgacatg gcggaaggtc tgccagcgat
      541 gcgcgcccag gctcgcggcg gcctcctcga gttcggtgtc gatgtcttcg agcaccggct
      601 gcaccgtgcg cacgacgaac ggcaggccga tgaagaccag cgccaccagc acgcccagcg
      661 gcttgaacgc gaccttgatg ccgagcggct cgagcaggcc cccgagccag ccgttgccgg
      721 cgtacagcgc cgtcagcgcg atgccggcca ccgccgtggg cagcgcgaac ggcaggtcga
      781 tcagcgcgtc gacgaggcgc ttgccgggga acgagtagcg caccagcgac caggccagca
      841 gcagcccgaa cacgctgttg atcgccgccg ccagcagcga ggcaccgaag ctcagctggt
      901 acgaggcgac gacgcgcggc gcggtgacga cgttccagaa cggctcccag cccagcgtcg
      961 cggtcttgaa gaacaccgcc gacagcggga tcagcacgag cagcgacagg tacgcgatcg
     1021 tgtagccgag cgagagcccg aagccgggca gcacgcggcg cggctttcgc ttgggcagcg
     1081 ccccggccga tggcgccggg aacggcgcga gcacggcgct catcaggacc gcctcagcac
     1141 gagctggtcg tacagaccgc cgtcgccgaa gtgcttcttg aagaccgcgc cccagccgcc
     1201 gagcagcgag tcgaccgtga aggtcttgac cggcgggaag cgctcggcgt tcgccttcag
     1261 caccgccggg tcgcgcgggc ggaagtcgtg ccgggcaatg atctgctggc cgaccggggt
     1321 gaacaggaag tcgaggtagg cctgcgccgc ggcgcgcgtg ccgcgcttgg cggtgacctt
     1381 ctcgttcacc gccaccggcg cctcggcctc gatcgtgagg gtcgggtaga caatgtcgaa
     1441 ctggtcgcgg ccgacctcgc gcgcgatcag cgcggcctcg ttctcgaaag tcacgagcac
     1501 atcgccgatc tggcgctgcg tgaacgtggt ggtggcgccg cggccgccac cgtcgagcac
     1561 cggcacattg gcgagcagct tcgcgacgaa ctccttcgcc tgcgcctcgt tgccgccgcg
     1621 cgcgatcacc gagccccagg cgccgaggta ggtgtagcgg ccgttgcccg aggtcttcgg
     1681 gttcgggatg atcaccgcca ggccgggccg cgccagatcg gcccagtcga ggatcttctt
     1741 cgggttgccc ttgcgcacca ggaacaccga ggtcgtcgtg tagggcgccg caccgaacgg
     1801 gaagcgcttc ttccagtccg gggcgaccag cccgcgctcg gcgagcacgt cgatgtcggt
     1861 cgactggttc atcgtcacca cgtcggcctc gagcccgttc gcgaccgcct gggcctgcgc
     1921 ggtggagccg ccgtgcgact ggttgatcgc gagcgtcttg cccgtcgcgg ctttccactg
     1981 cgcgacgaac gccgggttca cctccttgta gagctcgcgg ctcacgtcgt acgagacgtt
     2041 cagcagcgtc tcggcgggcg cctgggcgcg cgcggcgccg gcgagcgcaa ggctcgcgaa
     2101 gagaacaggc aggaacagct tcttggacat ggcaatggcg gcgcgcggag aaccacgggt
     2161 gacgggaccg cgcaggttag gtgccggcgc gcggcgtgtg aaccatcgat tcggaatttc
     2221 catatccatg gaatcgatcg caggttttca gctaagcgcg tgccgacgcg ccgccagggc
     2281 acactgcgcg catgacgacg aacgacgctt tgcgcgtggt gtgcctgtgc gccggctggt
     2341 gcggcgtgtg ccgcgactac cgcgcgacct tcgacgccgc cgcggcgtcg ttcggcgcgc
     2401 gcgccgcgtt cgcctggttc gacatcgagg acgacgcggc gctgctcgac gacgtcgatg
     2461 tcgagaactt cccgaccgtg ttgatcgcgc acggcgacca cctgctgttc ttcggcacga
     2521 tcacaccgca gccggcgacg ctcgcgcggc tggtgcagcg cgcgctggcc ggcgaactgc
     2581 ggccgcaagc ggcggcgccc gaggtcgacg ccttcgtgca gcgcgcgcgc gcggccggcg
     2641 cctgacacgg cacgctcagc cgcgcttcgg gtagcgcggg ttcggcgcga tcgctgccag
     2701 gtcgaagctg ctgcgcgtgc cgttgaagtt ccagacgaag ctcttggcgc cgacctcgaa
     2761 gcggatcacc tcgccgccgg tgacgttgac atagccggtg tcgggccgga tcacgatcgt
     2821 gcgcgtgacc atcgcgacgc cggcggcgct gccgaacggg tcggagccgg ggtcgcgcgc
     2881 ggccagcacc tcgggggcgg gcgtcgatcc ggaccgatcc cacgcgagat agccgagccc
     2941 gccgcaggcg agccacgtgc gtcagctcga cgccgttgcg gaacgcgagc tccttcaggt
     3001 cgtcgggcag ttgctcggcg cgcggcatcg acgcgtcgtg caccagcacc gggatcaccg
     3061 ggatgtcgcg cttgagcgcg ctcgcggtct cgagccgcac gaaatcggcc gggtcgtcga
     3121 ggcggcgctt gccgctcggg tcggtggccg tgagccggct ccggccgatc accgcgagca
     3181 gcacgccgca ggacgcgacc tgctgctcga tcacgcggcg gaagtcgcgg cccggctcga
     3241 tgccggcgac gtccatgaac accgcgccct tgccgaagcg gtcgcacaag tcctcgaaca
     3301 ggtggccggc gtggccctcg gtgtcgtccc ggcgatagct aatgaagatc gcgcgcatcc
     3361 gcgcattgtg cggaagcgcg cgcgcggcga acccccagtc tggcggcaga atggggcggt
     3421 cagcaacgga gggaaccatg gcgtacatgc tgttgatcct ggagccgcgc ggccagcgcg
     3481 ccgagcgcgg gctggaggcc ggcaagcagg tctatgcgca gatgctgggc tttgcggaag
     3541 ccctgaagca gcgcggcgtg ctgcaggcgg tcgagtcgct cgcgaccgac gcgaccgcga
     3601 cccg
//
LOCUS       DASRDG010000608         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_665013, whole genome shotgun sequence.
ACCESSION   DASRDG010000608 DASRDG010000000
VERSION     DASRDG010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665013"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VFA35_10185"
     CDS             complement(<1..1033)
                     /locus_tag="VFA35_10185"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZT56585.1"
                     /translation="MAARIGIDTGGTFTDVVRWSPRGVVVHKLPSTPDDPGLAVLRGL
                     AAVRRRPDEPVDVVHGTTVGLNAVLQGRLPRTLFATNKGFRDLIEIGRQARTDLYALE
                     PDRPQPPVPRALRVEVDCRRGPDGAVERALTARAISDLVARARALRPEAIAIGFLHAP
                     AAPADERRCAAALRRALPDVPVSCSAEVLPAFGEYERFSAAILNAAIAPLVGRYTARL
                     SRAIGAGRLRLLRSSLGILPPAEAERFPARAMFSGPAGGVLATRRLCAAAGLPRAVAF
                     DMGGTSTDACLVLPDSVPTDQGEIAGLPLPLPTVDVHTVGCGGGSIAHRDRGGALCVG
                     PHSAGADPGP"
     gene            1111..1731
                     /locus_tag="VFA35_10190"
     CDS             1111..1731
                     /locus_tag="VFA35_10190"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HZT56586.1"
                     /translation="MGLVLGCVCAFALGSVPFALLLVRALKGVDVRTVGSGNVGATNA
                     SRAFAGRGARLAVFLLIYLLDAAKGFAPTWFGPALVGGTPQLDGVLLGAAAILGHVFP
                     PALRFRGGKGVATTTGVLLALDWQVTALALAAFFAVRWITGEVFLGSLALGVALTAAA
                     IALHPAAAFAARLPLTLLCCALTLFLFWTHRGNLRRRFAAARDGAR"
     gene            1728..>2294
                     /locus_tag="VFA35_10195"
     CDS             1728..>2294
                     /locus_tag="VFA35_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HZT56587.1"
                     /translation="MKIAVLGNGGFGTAMAITLAAAGRTVALWGHDVGYTQQLARTRQ
                     NPRYLEGVVLPAAVLVATDPAPVLEGADAVLVAVPTQHVRSTLVPMAALLAPPVAIVS
                     LAKGLEQATALRPSEVIAAVCGGSERVLVLSGPSHAEEIARGLPTTVVLAGPQEQVVH
                     RLQDQLATPAFRIYRNQDVLGTELCGALK"
BASE COUNT          256 a          865 c          889 g          284 t
ORIGIN      
        1 cgggccccgg atcggcgccg gcgctgtgcg ggccgacgca cagcgcgccg ccgcggtcgc
       61 ggtgcgcgat gctgccgccg ccgcagccga ccgtgtgcac gtcgaccgtc ggcagcggca
      121 gcggcagacc ggcgatctcg ccctggtcgg tgggcacgga gtcgggcagc acgaggcacg
      181 cgtcggtcga ggtgccgccc atgtcgaacg ccacggcgcg cggcaggccc gcggccgcgc
      241 acaggcgtcg ggtcgcgagc acgccgccgg ccgggcccga gaacatcgcg cgcgcgggga
      301 accgctccgc ctccgcgggc ggcaggatgc cgaggctcga gcgcaggagg cgcagccggc
      361 cggcgccgat cgcgcgcgac aaccgcgccg tgtagcggcc gacgagcggc gcgatcgccg
      421 cgttgaggat cgccgccgag aagcgctcgt actcgccgaa cgcgggcagc acctcggcgc
      481 tgcacgagac cggcacgtcg gggagtgcgc ggcgcagcgc cgccgcgcag cggcgttcgt
      541 ccgccggcgc cgcgggcgcg tgcaggaagc cgatcgcgat cgcctcgggc cgcagcgcgc
      601 gcgcgcgggc cacgaggtcg gagatcgcgc gcgccgtcag cgcgcgttcg accgcgccgt
      661 cggggccgcg ccggcagtcg acctccacgc gcagcgcgcg cggcaccggc ggctgcggcc
      721 ggtcgggctc cagcgcgtag aggtcggtcc tggcctggcg gccgatctcg atcaggtcgc
      781 ggaagccctt gttggtcgcg aacagcgtcc gcggcaggcg gccctgcagc accgcgttga
      841 gcccgacggt ggtgccgtgc acgacgtcga ccggctcgtc cgggcgccgc cgcaccgccg
      901 cgaggccgcg cagcaccgcg agccccggat cgtccggcgt cgaaggcagc ttgtgcacga
      961 ccacgccgcg cggcgaccac cgcacgacgt cggtgaacgt gccgccggtg tcgatgccga
     1021 tgcgcgccgc catggtcgcg cgacggtagg gacgccgcgc ggaaagtcga gagttggcgc
     1081 gcgggccgct gcccgcatac gttctcggcc gtgggcctgg tcctcggttg cgtgtgcgcg
     1141 ttcgcgctcg gcagcgtgcc gttcgcgctg ctgctcgtgc gcgcgctcaa gggcgtggac
     1201 gtgcggaccg tcggctcggg caacgtcggc gccaccaacg cgagccgcgc gttcgccggc
     1261 cgcggcgccc ggctcgccgt gttcctgctg atctatctgc tcgacgccgc gaaagggttc
     1321 gcgccgacgt ggttcggacc ggcgctggtc ggcggcactc cgcagctcga cggcgtgctg
     1381 ctcggcgccg ccgcgatcct cggccacgtg ttcccgccgg cgctccgttt ccgcggcggc
     1441 aagggcgtgg cgacgaccac cggcgtgctg ctcgcgctcg actggcaggt gaccgcgctc
     1501 gcgctcgcgg cgttcttcgc cgtgcgctgg atcaccggcg aggtgttcct cggcagcctc
     1561 gcgctcgggg tcgcgctgac ggccgcggcg atcgcgctgc accccgcggc ggcgttcgcg
     1621 gcgcggctgc cgctgacgtt gctctgctgc gcgctcacgc tgttcctgtt ctggacccac
     1681 cgcggcaacc tgcggcgccg gttcgccgcg gcgcgggacg gtgcgcgatg aagatcgccg
     1741 tgctcggcaa cggcggcttc ggcaccgcga tggcgatcac gctcgcggcg gcgggccgca
     1801 cggtggcgct ctggggccac gacgtcgggt acacgcagca gctcgcccgc acgcgccaga
     1861 acccgcgcta cctcgagggc gtcgtgctgc cggcggcagt gctcgtcgcg accgacccgg
     1921 cgccggtgct ggaaggcgcc gacgcggtgc tggtcgcggt gccgacgcag cacgtgcgca
     1981 gcacgctggt gccgatggct gcgctgctcg cgccgccggt cgcgatcgtc tcgctcgcga
     2041 aagggctcga gcaggcgact gcgctgcgcc cgagcgaggt catcgccgcc gtctgcggcg
     2101 gcagcgaacg cgtgctcgtg ctcagcggcc cgagccacgc cgaggagatc gcgcgcggcc
     2161 tgccgaccac ggtcgtgctc gccggcccgc aggagcaggt cgtgcaccgg ctccaggacc
     2221 agctcgcgac cccagcgttc cggatctacc gcaaccagga cgtgctcggc accgagctgt
     2281 gcggcgcgct caag
//
LOCUS       DASRDG010000609         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1699921, whole genome shotgun sequence.
ACCESSION   DASRDG010000609 DASRDG010000000
VERSION     DASRDG010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699921"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1547
                     /locus_tag="VFA35_10200"
     CDS             93..1547
                     /locus_tag="VFA35_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZT56588.1"
                     /translation="MDTPALFEIAREAMRQRGLQPDFSAAAQQEASAMTEPVAPPGAA
                     LRDLRELLWCSIDNDDSRDLDQLTVALPEADDVQRILVAIADVDALVHDGGAIDAHAR
                     TNTTSVYTAAGVFPMLPLRLSTDLSSLNENEDRQAIVVEIAIGAPGASSAPQVYRALV
                     RNRAQLSYNAVAAWLDGKAPVPPKVAALPGLEAQLRTQDRAGQALKEMRRRHGALSLA
                     TPEARPVFDGDGVLTDLRVDDGNRAKELIAGFMIAANGVTARFLDERNFPSLRRFLRE
                     PARWQKIAEIAAGFGAELPAEPSPIALDGFLTQRRLAAPEKFAELSLTIVKLLGSGEY
                     MLSRPGQRAPGHFGLAVNDYTHSTAPNRRYPDLITQRLLKAALRGAAPPLGDAELTQL
                     AQHCTEQEDNANKVERQVRKSAAALLLAARIGQSFNAIVTGVSAKGTWVRIASPVAEG
                     RVVQGYEGLDVGDKLHVTLVALDVARGFIDFKRV"
     gene            complement(1620..>2596)
                     /locus_tag="VFA35_10205"
     CDS             complement(1620..>2596)
                     /locus_tag="VFA35_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZT56589.1"
                     /translation="FNHPMNQVAHKVVPSVASNNRMVLKPSEKVPLSAYYFADLLYEA
                     GLPPEMLQVVTGDPREIADELLTHRHVDLITFTGGVPIGKYIAAKAGYRRIVLELGGN
                     DPLIVMDDADLDKASELAVQGSYKNSGQRCTAVKRMLVHQKVLGEFTERVVAKTRDWR
                     HGDPADKANEMGTVIDEAAARSFEKLVDEAVAQGARLLVGNRREGALYSPTVLDRVRP
                     EMTVVREETFGPVSPIIGFKDIDEAIRISNGTAFGLSSGICTNRMDDITRFARELQVG
                     TVNLWEVPGYRIELTPFGGIKDSGLGYKEGVQEAIKSFTNGKTFTLPW"
BASE COUNT          380 a          922 c          900 g          394 t
ORIGIN      
        1 caggtgggtc aatggcttca tgggcgggct ccgtcaggtg cgactgtcac acaatcggcg
       61 ctccagcacc ttcgcgagcc cccgcaccgc cgatggacac ccccgcactt ttcgagatcg
      121 cccgcgaggc gatgcgccag cgcgggctgc aacccgactt ttccgccgcg gcgcagcagg
      181 aggcctccgc gatgaccgag ccggtcgcgc cgccgggcgc ggcgctgcgc gacctgcgcg
      241 agctgctctg gtgctcgatc gacaacgacg actcgcgcga tctcgaccag ctcaccgtcg
      301 cgctgcccga ggccgacgac gtgcagcgca tcctcgtcgc gatcgccgat gtcgacgcgc
      361 tggtgcacga cggcggcgcg atcgacgcgc acgcgcgcac caacacgacc tcggtctaca
      421 cggccgcggg cgtgttcccg atgctgccgc tgcggctgtc gaccgacctg agctcgctca
      481 acgaaaacga agaccggcag gccatcgtgg tcgagatcgc gatcggtgcg ccgggcgcat
      541 cgagcgcgcc gcaggtctac cgcgcgctgg tgcgcaaccg cgcgcagctg agctacaacg
      601 ccgtcgccgc ctggctcgac ggcaaggcgc cggtgccgcc caaggtcgcg gcgctgccgg
      661 ggctcgaggc gcaactgcgc acgcaggacc gcgccggcca ggcgctgaag gagatgcgcc
      721 gccggcacgg cgcactgagc ctggcgacgc ccgaggcgcg gccggtgttc gacggcgacg
      781 gcgtgctgac ggacctgcgt gtcgacgacg gcaaccgcgc caaggagctg atcgccggct
      841 tcatgatcgc ggccaacggc gtgaccgcgc gctttctcga cgagcggaac ttcccgtcgc
      901 tgcgccgctt cctgcgcgag ccggcgcgct ggcagaagat cgccgagatc gccgcgggct
      961 tcggcgcgga actgcccgcc gaaccgagcc cgatcgcgct cgacggcttt ctcacgcagc
     1021 gccgcctggc cgcgcccgag aagttcgccg agctctcgct gaccatcgtc aagctgctcg
     1081 gctcgggcga gtacatgctg tcacgccccg gccagcgcgc gcccggccac ttcggcctcg
     1141 cggtcaacga ctacacccac tcgaccgcgc cgaaccggcg ctacccggac ctgatcacgc
     1201 agcgtctgct gaaggccgcg ctgcgcggcg ccgcgccgcc gctgggcgac gccgaactca
     1261 cgcagctcgc gcagcactgc acggagcagg aggacaacgc gaacaaggtc gagcgccagg
     1321 tgcgcaagtc ggccgcggcg ctgctgcttg ccgcgcgcat cggccagagc ttcaacgcga
     1381 tcgtcacggg cgtgtcggcc aagggcacgt gggtgcgcat cgcgtcgccg gtcgccgaag
     1441 ggcgggtggt gcagggctac gaggggctgg acgtgggcga caagctgcac gtcacgctgg
     1501 tggcgctcga tgtggcgcgg gggttcatcg acttcaagcg ggtgtgaacc cgccgcggcg
     1561 cggtttgcgc ccatctcttg ccgctatcgc acgagggccc gcaccagact gccggcccgc
     1621 taccagggca aggtgaacgt cttgccgttg gtgaaggatt tgatggcctc ctgcaccccc
     1681 tctttgtagc ccaagccgga gtccttgatg ccgccaaagg gcgtcagctc gatccggtag
     1741 cccggcactt cccagaggtt gacggtgccg acctggagct ccctggcgaa gcgggtgatg
     1801 tcgtccatgc ggttggtgca gatgcccgac gacaggccga aggccgtgcc gttggagatg
     1861 cggatcgcct cgtcgatgtc cttgaagccg atgatcggcg acaccgggcc gaaggtctct
     1921 tcgcgcacca cggtcatctc ggggcgcacg cggtcgagca ccgtcggcga gtacaaggcc
     1981 ccttcgcgcc ggttgccgac cagcagccgt gcaccctgcg ccaccgcctc gtcgaccagt
     2041 ttctcgaacg agcgcgccgc ggcctcgtcg atcacggtgc ccatctcgtt ggccttgtcg
     2101 gccgggtcgc cgtggcgcca gtcccgggtc ttcgcgacga cccgctcggt gaactcgccg
     2161 agcaccttct ggtgcaccag catgcgcttg accgcggtgc agcgctggcc ggagttcttg
     2221 tacgagccct gcaccgccag ttcgctggcc ttgtccaggt cggcgtcgtc catcacgatc
     2281 agcgggtcgt tgccgccgag ctcgagcacg atgcggcggt agccggcctt ggcggcgatg
     2341 tacttgccga tcggcacgcc gccggtgaag gtgatcaggt cgacatgccg gtgcgtcagc
     2401 aactcgtcgg cgatctcgcg cgggtcgccg gtcacgacct gcagcatctc gggcggcagc
     2461 ccggcttcgt agagcaggtc ggcgaagtag taggccgaca gcggtacctt ctccgagggc
     2521 ttgagcacca tgcggttgtt gctggcgacg ctgggcacca ccttgtgcgc gacctggttc
     2581 atcgggtggt tgaacg
//
LOCUS       DASRDG010000610         5490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1700088, whole genome shotgun sequence.
ACCESSION   DASRDG010000610 DASRDG010000000
VERSION     DASRDG010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700088"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFA35_10210"
     CDS             complement(<1..295)
                     /locus_tag="VFA35_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZT56590.1"
                     /translation="MKILVTGSAGHLGEAMMRGLRGTPHEAHGIDLLASPFTTQVGSI
                     ADRAFVARAMRGVDAVLHAATLHKPHVATHSKQDFIDTNLTGTLNLLEAAAAAQ"
     gene            399..707
                     /locus_tag="VFA35_10215"
     CDS             399..707
                     /locus_tag="VFA35_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56591.1"
                     /translation="MKTFAVSKVRLDPDGRVTHVYWGEVDTRKNDWAAPEAVAPVIDV
                     VRAIHDGNQVFALFPSTHGHLPERRFIVADYDNGWETIVLDGPPMDEREVHDMSRLPA
                     "
     gene            725..1927
                     /gene="pcaF"
                     /locus_tag="VFA35_10220"
     CDS             725..1927
                     /gene="pcaF"
                     /locus_tag="VFA35_10220"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HZT56592.1"
                     /translation="MTPAFICDAIRTPFGRYGGALSTVRADDLAAVPIRALMARNPGV
                     DWAAVTDVVYGCANQAGEDNRNVARMAGLLAGLPPDVAGTTLNRLCGSGMDAVGTAAR
                     AIKAGEAQLMIAGGVESMSRAPFVMPKAESAFSRANAVYDTTLGWRFVNRLMRQQYGV
                     DSMPETAENVAAEFKIERAAQDRMALASQLKALAAQRSGFFDAEITPVSVPQRKGEPL
                     SVTQDEHPRATSLEALATLKGVVRPDGTVTAGNASGINDGACALLLASEAAAAKHGLT
                     PRARVVGMAVAGVAPRIMGFGPAPATRKLLELTGLALAQMDVIELNEAFAAQGLAVLR
                     DLGLADDDARVNPNGGAIALGHPLGASGARLVTTAVNQLHKTRGRYALCTMCVGVGQG
                     IAIVVERV"
     gene            complement(1938..2210)
                     /locus_tag="VFA35_10225"
     CDS             complement(1938..2210)
                     /locus_tag="VFA35_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56593.1"
                     /translation="MESHGAHRDGPHRPPARYLVVIDAGGEQVARLFDARRTQVAEFD
                     AGTEEVAVMAKGLAPQPGADAAEWDAALASHSATERAAADIYLLDL"
     gene            2311..3441
                     /locus_tag="VFA35_10230"
     CDS             2311..3441
                     /locus_tag="VFA35_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZT56594.1"
                     /translation="MKSLGKKPDGERLERIKASKLWAGESFRNLHPIAPGLRDPAAPK
                     PTLSEFLCGGPRRVPAAPLPSMNPLDAWRRPPETGLRATWLGHSTVLIEIDGLRVLTD
                     PVWGPRASPSRIAGPKRFQPVPIALRAMPPIDLVLVSHDHYDHLDYPTIRELATMGVP
                     FVTSLGVGAHLVAFGVPPERITELDWWESHTVIGQGRGGTELTVTAAPSQHFSGRGLK
                     DRNATLWSSFAIRTPKHSVFFSGDTGLTSEYATIRERLGPFDLVMLEVGAFHPSWGDI
                     HLGPEHALEAYQLLGGGAFLPVHWGTFALAVHDWDAPAETLLELGPKQGAHLVMPRLG
                     EAVEPARAEAVTPWWRAVDRRDEPAPEPEPMTLPKTMAWPVD"
     gene            complement(3451..3978)
                     /locus_tag="VFA35_10235"
     CDS             complement(3451..3978)
                     /locus_tag="VFA35_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZT56595.1"
                     /translation="MFKTVALVIAALIGGVLVFASTKPDSFSVQRSASIKAPPEKIFA
                     VLNDFHRWTEWSPWEKLDPAMKRTLGGAASGKGATYAWEGNSKAGQGRMEIIESAPAS
                     KVGIQLDFIKPFEGHNIAEFTLTPQGDATRVDWVMRGPTPFVSKLMQVFVSLDAMIGK
                     DFEEGLANLKRVTEK"
     gene            complement(3978..4391)
                     /locus_tag="VFA35_10240"
     CDS             complement(3978..4391)
                     /locus_tag="VFA35_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZT56596.1"
                     /translation="MQFSTYLFFDGRCEEAAAFYQQAIGAELVFMMRYSDSPEPHPPG
                     MVPPGFESKVMHMTLKIGDNTVMAADDCTGHPKFTGFSLSLAVKTEAEADKLFAALGD
                     GGSVTMPLTKTFFAPKFGMLTDRFGVGWMVLVGDK"
     gene            complement(4436..>5490)
                     /locus_tag="VFA35_10245"
     CDS             complement(4436..>5490)
                     /locus_tag="VFA35_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZT56597.1"
                     /translation="LLEHLQGRRSRQRLVRTFVREAETGAVKYVNWIEQDIAFAEVGT
                     PTWDGLPLDRYLSLLDMLNPMHRLWSDGRWNKLTVAHGCYWKKCSFCDVSLDYISRYE
                     GASAETLVDRIEAIVAETGQTGFHFVDEAAPPKALRALAAELQRRRLAISWWGNIRFE
                     KSFSPALCRELADSGCIAISGGLEVASDRLLALMKKGVSVEQVARVTKGFADAGILVH
                     AYLMYGFPTQTVQDTVDALEYVRQLFETGCIQSGFFHRFACTVHSPVGQNPADYGVTL
                     QPLPPVSFAKNDIGFIDPTGTDHDALGHALKKALYNYMHGIGLDEDVRSWFDLPVPKT
                     KVARHRIARALALAPA"
BASE COUNT          820 a         1913 c         1919 g          838 t
ORIGIN      
        1 cctgcgcggc ggcggccgct tcgagcaagt tcagcgtgcc ggtgaggttg gtgtcgatga
       61 aatcttgctt gctgtgggtg gcgacatgcg gcttgtgcaa ggtggccgcg tgcagcaccg
      121 catcgacgcc gcgcatcgcg cgcgcgacga acgcgcggtc ggcgatcgag ccgacctgcg
      181 tcgtgaaggg cgaggcgagc aggtcgatgc cgtgcgcctc gtgcggcgtg ccccgcagcc
      241 cgcgcatcat cgcctcgccg aggtggccgg cgctgccggt cacgagaatc ttcatccgct
      301 tggccttctg tggacgccgc gaaagcggca agtctacggt ctggacgctc ccgggtgcag
      361 ccgctatcct cgttcgcagt gaaccccttg gagcgcgcat gaaaaccttc gccgtctcga
      421 aagtcaggct cgaccccgac gggcgcgtga cccacgtcta ctggggcgag gtcgacacgc
      481 gcaagaacga ctgggccgcg cccgaggcgg tcgcgccggt gatcgatgtg gtgcgcgcga
      541 tccacgacgg caaccaggtg ttcgcgctgt tcccctcgac ccacgggcac ttgcccgagc
      601 gccgtttcat cgtcgccgac tacgacaacg gctgggagac gatcgtgctc gacggcccgc
      661 cgatggacga gcgcgaagtg cacgacatgt cgcgcctgcc ggcgtgaccg caacccgccc
      721 ggccgtgacc ccggccttca tctgcgacgc gatccgcacg ccgttcggcc gctacggcgg
      781 cgcgctcagc accgtgcgcg ccgacgacct cgcggcggtc ccgatccggg cgctgatggc
      841 gcgcaacccc ggcgtcgact gggcggcggt caccgacgtg gtctacggct gcgcgaacca
      901 ggccggcgaa gacaaccgca atgtcgcgcg catggccggg ctgctcgccg gcctgccgcc
      961 cgatgtggcc ggcacgacgc tgaaccgcct gtgcggctcg ggcatggacg cggtcgggac
     1021 cgcggcgcgc gcgatcaagg ccggcgaggc gcagctgatg atcgccggcg gcgtcgagag
     1081 catgagccgc gcgccgttcg tgatgcccaa agccgaaagc gcgttcagcc gcgctaacgc
     1141 ggtctacgac acgacgctcg gctggcgctt cgtcaaccgg ctgatgcggc aacagtacgg
     1201 cgtcgactcg atgcccgaga ccgccgagaa cgtcgcggcc gagttcaaga tcgagcgcgc
     1261 ggcgcaggac cgcatggcgc tcgcctcgca attgaaggcg ctggcggcgc agcgcagtgg
     1321 cttcttcgat gccgagatca cgccggtcag cgtgccgcag cgcaagggcg agccgctgag
     1381 cgtgacgcag gacgagcatc cgcgcgcgac cagcctggag gcgctcgcga cgctgaaagg
     1441 cgtcgtccgg cccgacggca cggtgacggc aggcaacgcc tcgggcatca acgacggcgc
     1501 gtgcgcgctg ctgctcgcga gcgaagcggc ggcggcgaag cacggcctga cgccgcgtgc
     1561 ccgggtcgtc ggcatggcgg tggccggcgt cgcgccgcgc atcatgggct tcggcccggc
     1621 gccggcgaca cgcaagttgc tcgaactcac cggcctggcg ctcgcgcaga tggacgtgat
     1681 cgagctgaac gaagccttcg ccgcgcaggg cctggccgtg ctgcgcgacc tgggcctcgc
     1741 cgatgacgat gcgcgcgtca acccgaacgg cggcgcgatc gcgctcggcc acccgctcgg
     1801 cgcgagcggc gcgcggctgg tgacgacggc cgtcaaccag ctgcacaaga cccgtggccg
     1861 ctacgcgctg tgcacgatgt gcgtcggtgt cggccagggc atcgcgatcg tggtcgagcg
     1921 cgtttgaaag ccggggccta gaggtcgagc aggtagatgt cggccgcggc gcgctccgtg
     1981 gcgctgtggc tcgcgagcgc ggcgtcccac tcggcggcat cggcgccggg ctgcggcgcc
     2041 aggcccttcg ccatcacggc gacttcttcg gtgccggcgt cgaactcggc gacctgggtg
     2101 cggcgcgcgt cgaacagccg cgcgacctgc tcgccacccg catcgatcac gaccaggtag
     2161 cgtgccggcg gccggtgcgg gccgtcgcgg tgcgcgccgt gcgattccat cccgaagccc
     2221 tgtcccatgc gctcatcttg ccacgcaata gggcgccatg ggggcgtatc gtgcgggttc
     2281 ggtcagctcc acgcggatac cccaagcgcc atgaaatcac tcggcaagaa acccgacggc
     2341 gaacggctcg aacgcatcaa ggcctcgaag ctctgggccg gcgagtcgtt ccgcaacctg
     2401 cacccgatcg cgcccggctt gcgcgacccg gcggcgccga agcccacgct cagcgagttt
     2461 ctgtgcggcg gcccgcgccg tgtgccggcc gcgccgctgc cgtcgatgaa cccgctcgac
     2521 gcgtggcgca ggccgcccga gaccggcctg cgcgccacct ggctcggcca ctcgaccgtg
     2581 ctgatcgaga tcgacggcct gcgcgtgctg accgacccgg tctggggccc gcgcgcctcg
     2641 ccgtcgcgca tcgccgggcc gaagcgcttc cagccggtgc cgatcgcgtt gcgcgccatg
     2701 ccgcccatcg acctggtgct ggtctcgcac gaccactacg accacctcga ctacccgacg
     2761 atccgggagc tggcgacgat gggcgtgccc ttcgtcacct cgctcggcgt cggcgcgcac
     2821 ctcgtggcct tcggcgtccc gcccgagcgc atcaccgagt tggactggtg ggagtcgcac
     2881 acggtgatcg gtcaagggcg agggggcacc gagctgacgg tcacggccgc gccctcgcag
     2941 cacttctcgg gccgcgggct gaaagaccgc aacgcgacgc tgtggtcgtc gttcgcgatc
     3001 cgcacgccga agcactcggt gttcttcagc ggcgacaccg gcctgacgag cgagtacgcc
     3061 accatccgcg agcgcctcgg cccgttcgac ctggtgatgc tcgaagtcgg cgctttccac
     3121 ccgtcgtggg gcgacatcca cctcgggccc gaacacgcgc tcgaggccta ccaactgctc
     3181 ggcggcggcg cgttcctgcc ggtgcactgg ggcaccttcg cactcgcggt gcacgactgg
     3241 gacgcgcccg ccgagacgct gctcgaactc gggccgaagc agggcgcgca cctggtgatg
     3301 ccgcgcctgg gcgaagcggt cgaacccgcg cgcgccgaag cggtcacgcc atggtggcgc
     3361 gcggtcgacc ggcgcgacga gccggcaccc gaacccgagc cgatgacctt gccgaagacg
     3421 atggcctggc cggtcgactg aagcccggct tcacttctcg gtgacgcgct tcaggttcgc
     3481 gaggccttcc tcgaagtcct tgccgatcat cgcgtcgagg ctgacgaaca cctgcatcag
     3541 cttcgagacg aacggcgtcg gcccgcgcat cacccagtcc acccgggtcg cgtcgccctg
     3601 cggcgtcagg gtgaactcgg cgatgttgtg gccctcgaac ggcttgatga aatcgagctg
     3661 gatgccgacc ttgctcgcag gtgccgattc gatgatctcc atgcggccct ggccggcctt
     3721 gctgttgcct tcccaggcgt aggtcgcgcc cttgccgctg gcggcgccgc ccagcgtgcg
     3781 cttcattgcc gggtcgagct tctcccaggg cgaccactct gtccagcggt gaaagtcgtt
     3841 cagcaccgcg aagatcttct cgggcggcgc cttgatcgag gccgagcgct gcacgctgaa
     3901 gctgtcgggc ttggtcgacg cgaacaccag cacgccgccg atcagcgcgg cgatgacgag
     3961 ggcgacggtc ttgaacatca cttgtccccg accagcacca tccagcccac gccgaagcgg
     4021 tcggtgagca tcccgaactt cggcgcgaag aaggtcttcg tcagcggcat cgtcacgctg
     4081 ccgccgtcgc cgagcgcggc gaacagcttg tcggcctcgg cctcggtctt gaccgcgagc
     4141 gaaagcgaga agccggtgaa cttcgggtgg ccggtgcagt cgtcggcggc catcacggtg
     4201 ttgtcgccga tcttcagcgt catgtgcatg accttgctct cgaagcccgg cggcaccatg
     4261 cccggcggat gcggctcggg gctgtcgctg tagcgcatca tgaagacgag ctcggcgccg
     4321 atcgcctgct ggtagaacgc ggcagcctct tcgcagcggc cgtcgaagaa caggtaggtg
     4381 ctgaattgca tggggtggcc ttctggatct tgttgaacga acgagaaaga tagcgctaag
     4441 ccggcgccag cgccaatgcc cgcgcgatcc gatggcgcgc gactttggtc ttcggcacgg
     4501 gcaggtcgaa ccagctgcgc acgtcttcgt cgagcccgat gccgtgcatg tagttgtaga
     4561 gcgccttctt cagcgcgtgg ccgagcgcgt cgtggtcggt gccggtcggg tcgatgaagc
     4621 cgatgtcgtt cttcgcgaaa ctcaccggcg gcagcggctg cagcgtgacg ccgtagtcgg
     4681 ccgggttctg cccgaccggc gagtgcaccg tgcacgcgaa gcggtggaag aagcccgact
     4741 ggatgcagcc ggtctcgaac aactggcgca cgtattcgag cgcgtcgacc gtgtcctgca
     4801 ccgtctgcgt cgggaagccg tacatcaggt aggcgtggac gaggatgccg gcgtcggcga
     4861 agcccttcgt cacgcgcgcg acctgctcga ccgacacgcc tttcttcatc agcgccagca
     4921 gccggtcgga ggccacttcg agcccgcccg agatcgcgat gcagccgctg tcggcgagct
     4981 cgcggcacag cgcggggctg aacgacttct cgaaccggat gttgccccac cacgagatcg
     5041 cgagccggcg acgctgcagc tcggcggcga gcgcgcgcaa agccttcggc ggcgcggcct
     5101 cgtcgacgaa gtggaagccg gtctggccgg tctcggcgac gatcgcctcg atgcggtcga
     5161 ccagcgtttc ggcggaggca ccttcgtagc gcgagatgta gtcgaggctc acgtcgcaga
     5221 agctgcactt cttccagtag cagccgtgcg cgaccgtcag cttgttccag cgcccgtcgg
     5281 accacagccg atgcatcggg ttcagcatgt cgagcagcga caggtagcgg tccagcggca
     5341 ggccgtccca ggtcggtgtg ccgacttcgg cgaaggcgat gtcttgttcg atccagttga
     5401 cgtacttcac cgcgcctgtt tcggcctcgc gcacgaaggt gcgcacgagg cgctggcgcg
     5461 agcgccggcc ctgcaggtgt tcgagcagcg
//
LOCUS       DASRDG010000611         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_326219, whole genome shotgun sequence.
ACCESSION   DASRDG010000611 DASRDG010000000
VERSION     DASRDG010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326219"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFA35_10250"
     CDS             <1..394
                     /locus_tag="VFA35_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56598.1"
                     /translation="VYGGAMLLLLGYLVHVYVGLPWWSRAHRPFTLAWRALRGIAPDD
                     PEPQWREAFHRLHAALNRTAGEVVFESGIDRFVAAQPRFEHLRDDLVTFFEQSRREFF
                     SQQPTARAERAWLIGFCRRCRDAERGSA"
     gene            391..1434
                     /locus_tag="VFA35_10255"
     CDS             391..1434
                     /locus_tag="VFA35_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HZT56599.1"
                     /translation="MSFAHPLLLSLLPLAVLPLLAQPAGALLATWPALTPRDRASEAL
                     GWALRGLGVLALAALVVGMTGPYRPEYDIERVGKGAEIVLVLDRSRSMDQGFAGARPA
                     GGATQGTGPQALDYYFSQSPGRLRDSKGKVARQMLSEFAAKRPDDRFGMIVFSTLPIR
                     VLEFTQKNDVIQAAIAAGNIGRGLSETNIGLALQSALSYFDDRPYTGSRIVMLVSDGG
                     DRLDVGARERVAYLARLHRVAIYWIYLRSANSPGLMLETGESPENADTVPEYVLHRYF
                     ESIGTSYHAYEASNPEALLKAIEAVNQLENLPITYIDTMPRRDLSAACFGAAFACVLL
                     LLTANLMEIRRWA"
     gene            1425..2051
                     /locus_tag="VFA35_10260"
     CDS             1425..2051
                     /locus_tag="VFA35_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MxaK protein"
                     /protein_id="HZT56600.1"
                     /translation="MGLSPDRRRKDAFLSDARPRGARVALLASWRRTRAVWLLLALVA
                     VAAAVDTVRLWQQQRWNDSIDALSVPARGNYKDLPPEVQFAQAYALAASGAGEAARQR
                     YNGLLDDTRLGQAARYNSANLLMREAVVVRASAQPGQAIALIELAKEGYRDVLRLDPQ
                     NWQARYNLERAQRLLADPDELLEAAPPESKRDAERAATTMRGFSPGLP"
BASE COUNT          293 a          732 c          716 g          335 t
ORIGIN      
        1 cgtctacggc ggcgcgatgc tgttgctgct cgggtacctg gtccatgtgt acgtcggctt
       61 gccgtggtgg tcgcgcgcgc atcgtccgtt cacgctggca tggcgcgcgt tgcgcggcat
      121 cgcccccgac gatcccgagc cgcaatggcg tgaggcgttt catcgcctgc acgcggcctt
      181 gaaccggact gcaggcgagg tcgtcttcga atcgggcatc gaccgcttcg tcgccgccca
      241 gccccgcttc gagcacctgc gcgacgacct ggtcacgttc ttcgagcagt cgcgtcggga
      301 gttcttctcg cagcagccga cggcgcgcgc cgaacgggcc tggttgatcg gcttttgccg
      361 acgctgtcgc gatgccgagc gaggatcggc atgagcttcg cgcacccctt gctgctgagc
      421 ctgctcccgc tggccgtgct gccgctgctc gcgcagcctg ccggcgcgct cctcgccaca
      481 tggccggcgt tgacgccgcg cgaccgcgcg tcggaagcgc tgggctgggc gctgcgcggc
      541 ctcggcgtgc tggcgctcgc ggcgctcgtc gtcggcatga ccggccccta ccggcccgaa
      601 tacgacatcg agcgggtcgg caagggtgcc gagatcgtgc tcgtgctcga tcgcagccgc
      661 agcatggacc agggcttcgc cggggcgcgt ccggccggcg gggcgacgca gggcacgggc
      721 ccccaggcgc tcgactacta cttcagccag tcgccgggtc gcctgcgcga ttccaagggc
      781 aaggtcgcgc ggcagatgct gtccgagttc gcggcgaagc gcccggacga tcgattcggc
      841 atgatcgtct tcagcaccct gccgatccgg gtgctcgaat ttacgcagaa gaacgacgtg
      901 atccaggccg ccatcgcggc cggcaacatc ggccgcggcc tgtccgagac caacatcggc
      961 ctggcgctgc aatcggcgct gtcctacttc gacgatcggc cctacaccgg ctcgcgcatc
     1021 gtcatgctgg tgtccgatgg cggcgaccgg ctcgacgtgg gcgcgcgtga gcgcgtggcc
     1081 tatctcgcgc gcttgcaccg agttgcgatc tactggatct acctgcgctc ggccaacagt
     1141 ccgggattga tgctggagac cggcgaatcg cccgagaacg ccgacaccgt gccggagtac
     1201 gttctgcatc gctacttcga gtcgatcgga acgtcgtacc acgcctacga ggccagcaat
     1261 cccgaggccc tgctcaaggc catcgaggcg gtgaatcagc tcgagaacct gcccatcacc
     1321 tatatcgaca cgatgccgcg ccgcgacctc tcggcggcgt gcttcggcgc cgccttcgcc
     1381 tgtgtgctgc tgctgctgac cgccaacctg atggagatcc ggcgatgggc ctgagcccgg
     1441 accgacgccg caaggacgcg ttcctgtcgg acgcaaggcc gcgcggcgcc cgggtcgcgt
     1501 tgctggcctc atggcgacgg acgcgcgccg tgtggctgct cctggcgctc gttgccgtcg
     1561 ccgcggccgt cgacacggtg cgcctctggc aacagcagcg ctggaacgac tcgatcgatg
     1621 cattgtcggt tccggcgcgg ggcaactaca aggacctgcc gcccgaagtg cagttcgcgc
     1681 aggcctacgc gctggctgcc agcggcgcgg gagaagcggc gcggcagcgc tataacggct
     1741 tgctggacga cacgcggctc ggccaggccg cgcgctacaa cagcgcgaac ctgttgatgc
     1801 gcgaggcggt cgtggtccgg gccagcgctc agcccggcca ggcgatcgcg ctgatcgagc
     1861 tggcgaagga gggctatcgc gacgtgctcc gcctcgaccc gcagaattgg caggcgcgct
     1921 acaacctgga gcgcgcgcag cgtctgctgg ccgatcccga cgagctgctc gaggccgcac
     1981 cacccgaatc gaagcgcgat gccgagcgcg cggccacgac catgcggggc ttctcgcccg
     2041 gcctgccatg acggcgcggg accgcctgcg caggtt
//
LOCUS       DASRDG010000612         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_326245, whole genome shotgun sequence.
ACCESSION   DASRDG010000612 DASRDG010000000
VERSION     DASRDG010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326245"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="VFA35_10265"
     CDS             <1..1445
                     /locus_tag="VFA35_10265"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZT56601.1"
                     /translation="GNVYFCADATDIFDCTGGMPLCTVAVSGGTMACSTNALSAGSHE
                     IRAYFPGDIDNEASSSRLTQLVGTAPAITSANATTFTVGVAGSFTVTTTGTPTPTVNA
                     GVVAFPAGVNFVDNGNGTATFSGTPAFGSVGAYNATLHAQNIVPPQATQTFTLTVVRA
                     NQTLTFPPQSPASHAFTAGGTFAISPPATSATPNSGNPIVYSSQTAGICSVAGTTVTM
                     LAIGTCTIAADQAGNADYAAAATVTQNVAIGLTSQTITFGPAPMVSVNGNGSVTATTT
                     ATPAANYPIVFSTASADCTVTTAGAVSGIRAGVNNCIIAATQAGDALYASASATQVLS
                     IGKKSTTVALAATPNPATINAAVTLTVTVAGDPPTGAVSFCDGATTVNATCAGGSTLC
                     ATVALTQGATNSSATCAVSFASAGTHSLSAYYAGDGNFSPAATGQVLALQVDPAALPA
                     VPAPLSRWTLLLLAGLLIAAAGLARARMRA"
     gene            1810..>2158
                     /locus_tag="VFA35_10270"
     CDS             1810..>2158
                     /locus_tag="VFA35_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZT56602.1"
                     /translation="MSTLGADALFDAVYARLKAMAGRQLAGRERGTLDTTALVHELYL
                     RINGQRDLQFAHPAQFFAYAARAMRHLLADRARGHLRQRAGGDWLRVTLSGVDAQIVT
                     DSAEQALALDAALR"
BASE COUNT          363 a          770 c          694 g          331 t
ORIGIN      
        1 ccggaaacgt ctacttttgc gccgacgcaa ccgacatctt cgactgcacc ggcggcatgc
       61 cgctgtgcac ggtcgcggtc agcggcggca cgatggcgtg ttcgacgaat gccctcagcg
      121 ccggatcgca cgagatccgc gcgtacttcc ccggcgacat cgacaacgag gcttcttcct
      181 cgcgcctgac ccaactcgtc ggcacggcgc cggcgatcac cagcgcgaac gccacgacat
      241 tcaccgtcgg cgtggccggc agtttcaccg tgaccacgac cggcacgccg acgccgacgg
      301 tcaacgccgg cgtcgtcgcg ttccccgccg gagtcaactt cgtcgataac ggcaacggca
      361 ccgcgacgtt ctcgggcacc ccggcgtttg gcagcgtggg cgcttacaac gcgaccttgc
      421 acgcgcagaa catcgtgccg ccccaagcca cgcagacgtt cacgctgact gtcgttcgcg
      481 ccaatcagac gttgaccttc ccgccgcagt cgccggccag ccacgcgttc actgcgggcg
      541 gcacgttcgc gatctcgcca ccggcgacgt cggcgacgcc gaattccggc aatccgatcg
      601 tttattcctc gcagaccgcc ggcatctgca gcgtggccgg cacgacggtg acgatgctcg
      661 cgatcggcac ctgcacgatc gccgccgacc aggccggtaa tgccgattac gccgccgcgg
      721 ctacggtcac gcagaacgtg gcgatcggac tcaccagcca gacgatcacg ttcggccctg
      781 cgccgatggt ttcggtgaat ggcaacggca gcgtgactgc cacgaccacc gcgacgcccg
      841 cggcgaacta cccgatcgtc ttctccacgg catcggccga ctgcacagtg accacggcgg
      901 gggcggtgag cggaattcgc gccggcgtga acaactgcat catcgcggcg acgcaggcgg
      961 gcgatgcgct ctacgcaagc gcgagcgcga cacaggtgct gagcatcggc aagaagtcga
     1021 ccacggttgc gctggccgcc acgccgaatc cggcgacgat caacgcagcg gtgacgctga
     1081 cggtgaccgt ggccggcgac ccgcccacgg gcgccgtcag cttctgcgat ggcgcgacca
     1141 cggtgaacgc gacgtgcgcc ggcggcagca cgctttgcgc cacggtggcg ctcacccaag
     1201 gcgcgaccaa ttccagcgcg acctgcgcgg tcagcttcgc gagcgcgggc acgcacagcc
     1261 tcagcgcgta ctacgccggc gacggcaatt tctcgccggc cgcaactggg caagtgctgg
     1321 cgctgcaagt cgatcccgca gcgctaccgg cggtgcccgc gccactcagc cgctggacgt
     1381 tgctgctgct tgcaggtctc ctgatcgctg cggccggcct cgcacgcgcg cgcatgcgtg
     1441 cgtgaaggac cagaaagccc atgcgcggcg acggaccgca cgcaggggct catcaaaatt
     1501 gcaaaaactc gcagcactgc gcgagtccgg atcggcaatc gcaacggtcg cctgaagcct
     1561 tgatcgcggg cgtcttgttc tgtcccaggg atgaacgcac aggcgccgcc gacacgccac
     1621 tgatccatcc gccgctcgcc tgggccagat ggagatgggc atatgctttg cccgccgccc
     1681 gggcaggcgt tgcaggcatt ttgcgctttg ctggctgtat agtgctccac aagggatggc
     1741 tgggcaggtt ccgctgcgta gcgcggactg ctaatggtgg gtgcggtgga accgatggca
     1801 gattctgcga tgtccacgct gggcgccgac gcgctgttcg atgcggtcta tgcgcgcctc
     1861 aaggccatgg ccgggcgcca actcgccgga cgcgaacgcg gcacgctcga cacgactgcg
     1921 ctcgtacacg agctttatct gcgcatcaac gggcaacgcg atctgcagtt cgcgcatccg
     1981 gcacaattct tcgcctatgc cgcacgcgcg atgcgccatc tgctcgccga tcgcgcgcgc
     2041 ggccatctgc gccagcgcgc cggcggcgat tggctacgcg tcacgctgag cggcgtcgat
     2101 gcgcaaatcg tcaccgacag cgccgagcaa gcgctcgcgc tcgatgccgc gttgcgcc
//
LOCUS       DASRDG010000613         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_95850, whole genome shotgun sequence.
ACCESSION   DASRDG010000613 DASRDG010000000
VERSION     DASRDG010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95850"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFA35_10275"
     CDS             complement(<1..719)
                     /locus_tag="VFA35_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56603.1"
                     /translation="MNHTHTTRAVLAALFALCAPLAAQSPLQLPMAADNCGPPGGTVY
                     FDLDVLTTVTFTQFDVNTLSLPGTAGSVNVWIGPHTWVGASGDPTAWTLAATGPVTAA
                     DVDTPSPCTLAQPFALGPGTYALAIEHVGLQPAYTNGDGTNQAFANSELSFRAGAATP
                     AAFAGPVASPRVFNGAIWYTLGGTPLPLASSIPFGTGCYAVQGSFSELFPAVPGQRTF
                     DLDNTTLHLVPNAQGGYDVTRT"
     gene            complement(731..2326)
                     /locus_tag="VFA35_10280"
     CDS             complement(731..2326)
                     /locus_tag="VFA35_10280"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZT56604.1"
                     /translation="MLPVPFRVAPRTFRRLKTLGVVLGLLGLTAATTAIIRDLTLVAD
                     LHAPAVPAHQDQNELLALVQGGRGHSAFDKAFTQGDRLFDAVFNALDGVGANVGQGQR
                     FTRMPRADLTGPGEWANHVPSRATGPNARGCVECHNEPVTDGAGGVAGNVVRDPRHSG
                     NVAQMIHRSTPHTFGMGALQRLAEEMTDELLGIRSALAAQVRASGTPASVPLAAKGVS
                     FGTLTALPGKGVGSAVFDTSAVQGVSSDLIVRPLQWKGSVAFLRDFNRDAANNELGMQ
                     AVELVGEGVDGDGDGVVDELSVGDITALTVYLAAQPRPVTKLELNELGLLPEPLTQTQ
                     IDQIGHGQQVFAQIGCASCHVPSLQLVDPVFKEPSSNANFRDARFPSGTLPRVAGVRA
                     DLPVSFDLRTDQPANVFVVNGQIVRLGGFRAGANGNGAVVELYGDLKRHDLGPELAEA
                     IDEVGTGASVFLTENLWGVGATAPYMHDGRATTLTEAILEHGGEGAASRAAFRALTTQ
                     EQLDMLLFLDNLTLIKGGLPVGG"
BASE COUNT          309 a          879 c          850 g          337 t
ORIGIN      
        1 gtgcgcgtca cgtcgtagcc gccctgcgcg ttggggacca ggtgcagcgt ggtgttgtcg
       61 aggtcgaacg tgcgctgccc cggcaccgcc gggaacagtt cgctgaagct gccctgcacg
      121 gcgtagcagc cggtcccgaa cgggatgctc gaggccagcg gcagcggcgt gccgccgagc
      181 gtgtaccaga tcgcgccgtt gaacacgcgc gggctcgcga ccggacccgc gaacgcagcc
      241 ggcgtcgcgg cgccggcgcg gaacgacagt tcgctgttgg cgaacgcctg gttggtgccg
      301 tcgccgttgg tgtacgcggg ctgcagcccg acgtgttcga tcgcgagcgc gtaggtgccc
      361 gggccgagcg cgaacggctg ggcgagcgtg cacgggctcg gcgtgtcgac gtcggccgcg
      421 gtcaccggcc ccgtggcggc gagcgtccag gccgtcgggt cgccgctcgc gccgacccag
      481 gtgtggggac cgatccagac gttgaccgag cccgcggtgc cgggcagcga cagcgtgttg
      541 acgtcgaact gcgtgaacgt gaccgtcgtc agcacgtcga ggtcgaagta gacggtgccg
      601 cccggcgggc cgcagttgtc ggccgccatc ggcagctgca gcggcgactg tgccgcgagc
      661 ggcgcgcaca gcgcgaacag cgcggccagc accgcgcggg tcgtgtgagt gtggttcatg
      721 tcaggtctcc tcagccgccc accggcaggc cgcccttgat cagtgtgaga ttgtcgagga
      781 acagcagcat gtcgagctgc tcctgcgtcg tgagcgcgcg gaacgccgcg cgcgacgcgg
      841 cgccctcgcc gccgtgctcg aggatcgcct cggtcagcgt cgtcgcgcgg ccgtcgtgca
      901 tgtacggcgc cgtcgcgccg acgccccaca ggttctcggt caggaacacc gacgcgcccg
      961 tgcccacctc gtcgatcgcc tccgccagct cgggacccag gtcgtgccgc ttcaggtcgc
     1021 cgtacagctc caccaccgcg ccgttgccgt tcgcgccggc gcggaagccg cccagccgca
     1081 cgatctgccc gttcaccacg aacacgttcg ccggctggtc ggtgcgcaga tcgaagctca
     1141 ccggcaggtc cgcgcgcacg cccgccaccc gcggcaacgt cccgctcggg aaccgcgcgt
     1201 cgcggaagtt cgcgttgctg ctcggctcct tgaacaccgg gtccacgagc tgcaggctcg
     1261 gcacgtggca ggaggcacag ccgatctgcg cgaacacctg ctgcccgtgc ccgatctggt
     1321 cgatctgcgt ctgcgtcagc ggctccggca gcagcccgag ctcgttcagc tcgagcttgg
     1381 tcaccggccg cggctgcgcc gccaggtaga ccgtcagcgc cgtgatgtcg ccgacgctga
     1441 gctcgtcgac gacgccgtcg ccgtcgccgt cgacgccctc gccgaccagc tcgaccgcct
     1501 gcatgccgag ttcgttgttg gccgcatcgc ggttgaagtc gcgcaggaac gccacgctgc
     1561 ccttccactg cagcggccgc acgatcaggt cgctgctcac gccctgcacc gccgaggtgt
     1621 cgaacaccgc gctgccgacg cccttgccgg gcagcgccgt cagcgtcccg aacgagacgc
     1681 ccttcgccgc cagcgggacg ctcgccggcg tgccggacgc gcgcacctgc gccgccagcg
     1741 cgctgcggat cccgagcagc tcgtccgtca tctcctccgc cagccgctgc agcgcgccca
     1801 tgccgaacgt gtgcggcgtg ctgcggtgga tcatctgcgc cacgttgccc gagtgccgcg
     1861 gatcgcgcac gacgttgccc gcgaccccgc cggcgccatc ggtcaccggc tcgttgtggc
     1921 actcgacgca gccgcgcgcg ttcgggcccg tcgcccgcga cggcacgtgg ttggcccatt
     1981 cgccgggccc cgtcaggtcg gcgcgcggca tgcgcgtgaa gcgctgcccc tggccgacgt
     2041 tggcgccgac gccgtcgagc gcgttgaaca ccgcgtcgaa cagccggtcg ccctgcgtga
     2101 acgccttgtc gaacgccgag tgcccgcggc cgccctgcac cagcgcgagc agctcgttct
     2161 ggtcctggtg cgccggcacc gccggcgcgt gcaggtcggc caccagcgtc aggtcgcgga
     2221 tgatcgcggt cgtcgccgcc gtgaggccga gcaggccgag cacgacgccc agcgtcttca
     2281 gtcggcggaa cgtgcgcggc gcaacgcgga acggaacagg gagcatggag tcgcctcggg
     2341 gattgctcgg aacgggatgc gggggtggag gcgcc
//
LOCUS       DASRDG010000614         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_802914, whole genome shotgun sequence.
ACCESSION   DASRDG010000614 DASRDG010000000
VERSION     DASRDG010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802914"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="VFA35_10285"
     CDS             complement(<1..1571)
                     /locus_tag="VFA35_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZT56605.1"
                     /translation="MSRLLLPLVLFAACARQPAPEPALTATPLRERAATPGPRFQRLD
                     PKTTGLDFTNTLQPQNTVPYVYVGGGVAIGDYDGDGLPDVFLCSLDGPNKLFKQVAPM
                     RFVDVTAQAGGPNGGLDGGDAWSTAAAFADVDGDGRLDLYVCNFESPNQLWLNNGDGT
                     FRECAGQFGLAITAACTGCAFADYDGDGDLDLYVVTDRMFGPALAQEIVDEVQLPTSV
                     KKTRAQLFPPYPRFERRDGKAVVPEGYEDFFAAVEDQIVAAGQRDYLMRNDGHGRFRD
                     ATEEAGIRGHGTGLSACWWDCDGDGRPDLYVANDLEAPDQLWHNRGDGTFEEITTKAL
                     PHVAYFGMGCDFGDIDGDGRFDFCVADMSSTTHYMGKMLMGSMGDKRWFLMHSDPQQY
                     MRNAVFLNTGTGRFLEAGYLLGLASTDWTWTVRFVDLDEDGRLDFYATNGIPRFEDNP
                     DTVAEFRRLLRSGHKAEALALARNLEPVLERNVARRNAGDLRFVDCGKEWGLDELGVS
                     QAAAFADLDRDGDLDL"
     gene            1659..>2229
                     /locus_tag="VFA35_10290"
     CDS             1659..>2229
                     /locus_tag="VFA35_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZT56606.1"
                     /translation="MSIAARVSIAVVLTAVFYAVALGLAGLLGFFVYAEFTHARHVNG
                     RAVIAAGVAMAVILWSVAPRIDRFPDPGVRLRRERQPKLWQLIEEVAQATAQPMPAEV
                     FLVTDVNAFVAQRGGLLGIGSRRVLGIGLPLLQLLTVAEVRAVLAHEFGHFHGGDTKL
                     GPWIYKTRGAVLRTVHNFAKVGSWLQLPFR"
BASE COUNT          293 a          752 c          830 g          354 t
ORIGIN      
        1 aggtcgaggt cgccgtcgcg gtcgaggtcc gcgaacgccg cggcctggct cacgccgagc
       61 tcgtcgaggc cccactcctt gccgcagtcg acgaagcgca ggtcgccggc gttgcggcgc
      121 gcgacgttgc gttccagcac cggctcgagg ttgcgcgcga gcgcgagcgc ctcggccttg
      181 tgccccgagc gcagcaggcg ccggaactcg gcgaccgtgt cggggttgtc ctcgaagcgc
      241 gggatgccgt tggtggcgta gaagtcgagc cggccgtcct cgtcgaggtc gacgaagcgc
      301 acggtccagg tccagtcggt gctcgccagc ccgagcaggt agcccgcctc gaggaagcgg
      361 ccggtgcccg tgttgaggaa caccgcgttg cgcatgtact gctgcgggtc gctgtgcatg
      421 aggaaccagc gtttgtcgcc catgctgccc atcagcatct tgcccatgta atgggtcgtg
      481 ctcgacatgt cggcgacgca gaagtcgaag cggccgtcgc cgtcgatgtc gccgaaatcg
      541 cagcccatgc cgaagtaggc gacgtgcggc agcgccttcg tggtgatctc ctcgaacgtg
      601 ccgtcgccgc ggttgtgcca cagctggtcg ggcgcttcga ggtcgttggc cacgtagagg
      661 tcgggccggc cgtcgccgtc gcagtcccac cagcacgccg acaggccggt gccgtggccg
      721 cggatgccgg cctcctcggt ggcgtcgcgg aaccggccgt ggccgtcgtt gcgcatcagg
      781 tagtcgcgct ggccggcggc gacgatctgg tcctcgaccg ccgcgaagaa gtcctcgtag
      841 ccttcgggca cgaccgcttt gccgtcgcgg cgctcgaagc gcggataggg cgggaacagc
      901 tgcgcgcgcg tcttcttgac gctcgtcggc agctgcacct cgtcgacgat ctcctgcgcc
      961 agcgcaggac cgaacatgcg gtccgtgacg acgtacaggt cgaggtcgcc gtcgccgtcg
     1021 tagtcggcga acgcgcagcc cgtgcacgcg gccgtgatcg cgagcccgaa ctggccggcg
     1081 cactcgcgga acgtgccgtc gccgttgttg agccagagct ggttcggcga ctcgaagttg
     1141 cagacgtaga ggtcgagcct gccgtcgccg tcgacgtcgg cgaacgccgc cgccgtgctc
     1201 cacgcgtcgc cgccgtcgag tccaccgttg gggccgccgg cctgcgcggt gacgtcgacg
     1261 aagcgcatcg gcgcgacctg cttgaacagc ttgttggggc cgtcgagcga gcagaggaac
     1321 acgtcgggca ggccgtcgcc gtcgtagtcg ccgatcgcga cgccgccgcc gacgtagacg
     1381 tagggcaccg tgttctgcgg ctgcaacgtg ttggtgaagt cgaggccagt ggtcttcgga
     1441 tcgagccgct ggaagcgcgg ccccggcgtg gccgcgcgct cgcgcagcgg cgtcgcggtg
     1501 agcgccggct cgggcgcagg ctgccgcgcg caggcggcga acaggacgag cggcaggagc
     1561 agacgggaca tccggcgccg atcttggtgg ccggcggtcg cggctgcaat ctccccggga
     1621 gctgcgcggc gaaacgcggc ggttaggatt ccctgcgcat gtcgatcgca gcgcgggtct
     1681 cgatcgccgt ggtgctgacg gcggtgttct acgccgtcgc actggggctc gcggggttgc
     1741 tcggcttctt cgtctatgcc gagttcacgc acgcccggca cgtgaacggc cgcgccgtga
     1801 tcgccgcggg cgtcgcgatg gcggtgatcc tctggtcggt ggcgccgcgc atcgaccgct
     1861 tccccgatcc cggcgtgcgg ctgcggcgcg agcggcagcc gaagctgtgg cagctgatcg
     1921 aggaggtcgc gcaggcgacc gcacaaccga tgccggccga ggtgttcctg gtcaccgacg
     1981 tcaacgcgtt cgtggcgcag cgcggcggcc tcttggggat cggcagccgc cgcgtgctcg
     2041 gcatcggact gccgctgctg cagctgctga cggtggcgga ggtgcgcgcc gtgctcgcgc
     2101 acgagttcgg ccacttccac ggcggcgaca cgaagctcgg cccgtggatc tacaagacgc
     2161 gcggcgcggt gctgcgcacg gtgcacaact tcgccaaggt cggctcgtgg ctgcagctgc
     2221 cgttccgct
//
LOCUS       DASRDG010000615         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1478541, whole genome shotgun sequence.
ACCESSION   DASRDG010000615 DASRDG010000000
VERSION     DASRDG010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478541"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFA35_10295"
     CDS             complement(<1..299)
                     /locus_tag="VFA35_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZT56607.1"
                     /translation="MRAALAAAAVAVAALTAQAPPQYRQLRYEEDWRYLRDPARAADG
                     LDAIKYMPLDDAGASWLSLGGEVRERYEYFHNSQWGGGPQDQNGYLLQRYMLHGDL"
     gene            complement(304..2163)
                     /locus_tag="VFA35_10300"
                     /pseudo
     CDS             complement(304..2163)
                     /locus_tag="VFA35_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
BASE COUNT          290 a          824 c          824 g          351 t
ORIGIN      
        1 aggtcgccgt gcagcatgta gcgctgcagc aggtagccgt tctggtcctg cggcccgccg
       61 ccccactgcg agttgtggaa gtactcgtag cgctcgcgca cctcgccgcc gagcgacagc
      121 cagctggcac ccgcgtcgtc gagcggcatg tacttgatcg cgtcgagccc gtcggcggcc
      181 cgcgccggat cgcgcaggta gcgccagtcc tcctcgtagc gcagctgccg gtactgcggc
      241 ggcgcctgcg ccgtgagcgc ggcgacggcg accgcggcgg ccgccagcgc cgcgcgcacg
      301 gggtcagaac gcgaagcagt cacagccctc ggctccgaac gcagagccag gcggccgctg
      361 cagcgccgcg gcgccggcgc gcacgcgctc gacgaacttc tcgaccgcgc ccttctccat
      421 cgagccgcgg tcgcgcgccg cggcctgcgc gtagccgccg taggtccgca ccggcgacca
      481 cgacgggctc gccggcggca gcggcggcgc cagcttctgg aagtcgccgg cgccgtgcac
      541 gacgcggccg ccgagcatcg tcagcgccga ctgcaggttc gggatctgct cctcggggat
      601 cgcgaagtag tccgcggtca gcacggcgag atccgcgagc tgaccctagg cgatcgcgcc
      661 cttcttcgcg tcctcgcccg agaaccacgc cgagcctgcg gtgtagaggc gcagcgccgt
      721 gctgcggtcg agccggttct gttgcggata gagcggcgtc ccgccgaccg tcttgccggt
      781 gaccagccag tgcagcgaca cgaacgggtt gtagctggcg acgcgcgtcg cgtccgtgcc
      841 ggcgccgacc gggatgccga gctcgagcat gcgccggatc ggcggcgtgc gctcggcggc
      901 cttgctgccg tagcgctcga cgaagtactc gccctggtac gccatgcggt gctggatggc
      961 gatgccgccg ccgagcgcgc gcacgcgttc gaggctcttg tcggtgatcg tctcggcgtg
     1021 gtcgaagaac cagcgcagcc cctgcagcgg cacgtcgcgg ttcacgcgct cgaacacgtc
     1081 gagggcgcgc acgatcgact cctcgtaggt cgcgtgcagc cggaacggcc accgcttctc
     1141 ggcgaggtgc cgcacgacgg cctcgagctc gccctcgagc tcccgcggca gatcgggccg
     1201 cggctcgagg aagtcctcga agtccgccgc cgagtagacc agcatctcgc cggcgccgtt
     1261 cacgcggtac aggtcgtcgc cctggcccgg ctccacctgc gcggtccagc gctggaagtc
     1321 cgcgagctcg cccttcggct tctgcgtgaa caggttgtag gcgatgcgca ccgtgagctc
     1381 gccgcggcgg tgcaggtcct cgacgaccgc gtagtcgtcg gggtagttct ggaagccgcc
     1441 gccggcgtcg atgatgctcg tgatgccgag ccggttgagc tcgcgctgga agtggcgcga
     1501 cgagttgcgc tggtcctcgg gcagcagctt gggccccttc gcgagcgtgc tgtagagcag
     1561 ccccgcgttc gggcgcgcga tcagcatgcc ggtcggctcg ccgcgcttgt cgcgctggat
     1621 ctcgccgccg ggcggatccg gcgcgtcctt gccgtagccg acggcgcgca gcgccgcgcc
     1681 gttgagcagc gcgcggtcgt agaggtgcag cacgaacacc ggcgtgtcgg gcgccgcggc
     1741 gttgatctcg gcgagcgtcg gcatccggcg ctccttgaac tggaactcgg tccagccgcc
     1801 gaccacgcgc acccactgcg gcggcggcgt gcgcgcggcc tgctccttca gcatgcgcag
     1861 cgcgtccgcc agcgacggca cgccgtccca gcgcagctcc atgttgtagt tgagcccgcc
     1921 gcggatcacg tggatgtgcg aatcgttgag gcccgggatc accgtgcggc cgcccagcgc
     1981 gatcacctgc gtcttggggc cgcgcaggcg catcacttcc tggtcggtgc cgacggccgc
     2041 gaacctgccg ccgcggatcg cgacggcggt cgccgacggt cgcgcgcggt ccatcgtggc
     2101 gatgcggccg ccggtgagga tgacgtcggc agattcggaa acggggttcg aagacgcgct
     2161 catggttgcc tccgcggttc tttggatcgg gaagcgggtc agcgcttctt cgccaccact
     2221 tgcggcttct gcgccgtctg cggcgccttg tgcaccatcg tgtacgcgta ctcgatgccc
     2281 gctccgtac
//
LOCUS       DASRDG010000616         4830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1247554, whole genome shotgun sequence.
ACCESSION   DASRDG010000616 DASRDG010000000
VERSION     DASRDG010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247554"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFA35_10305"
     CDS             <1..326
                     /locus_tag="VFA35_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56608.1"
                     /translation="IAFERAELRRQLTQVNAAARLPNLLRGVIGGNLGQALFGAAGPA
                     GQGGWVALALSLLRRYRVAAALLGSAAPMLRGRGGWRRVVRVGVLAAAAYFGWRLVQG
                     HERQR"
     gene            347..1738
                     /locus_tag="VFA35_10310"
     CDS             347..1738
                     /locus_tag="VFA35_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HZT56609.1"
                     /translation="MAPASAFVERRARLAAALRAAGGGVAVIPTAPERQRNSDNDHPF
                     RHDSSFHYLTGFDEPGAWLVLQASGETALGCHPNDLETAIWHGHRLGVEAGPAALGVD
                     AAFDVDTLDAVMLARLAGAPAVWFPFDAGPEVARRIDGWLATLRGRARSGVEAPAVQR
                     DLAPLVGEMRLVKDAGELATLRRAAQISADAHARAMRFCGARFRAAPDGTLREYEIEA
                     ELLHEFRRRGAQSPAYPSIVAAGANACVLHYAAGDAELRAGELCLIDAGCELDGYASD
                     ITRTFPANGRFTPAQRALYEIVFAAQQAAVDATRPGARQRDAHHAAVRVLTQGMLDSG
                     LLDRNAVGNVDDAIRSCAYRQFYMHGTGHWLGRDVHDVGEYLALDEPPGPQIDGMGRA
                     VVMKPSRVLVPGMVVTLEPGLYVRPAVGVPERFWHIGIRIEDDAVVTDTGCELISRGV
                     PVAAAEIEALMRA"
     gene            1758..3563
                     /locus_tag="VFA35_10315"
     CDS             1758..3563
                     /locus_tag="VFA35_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZT56610.1"
                     /translation="MDRRRLLRSAAAPLLAGLQPGALTNALAAPGPLPRVRPGDAAWP
                     SEARWAELGERVGGRLLALADPFKACRDAPAGAACAELFGSLKNPHVIGDSPALTQTL
                     GWVDAWTSRTSAYAVAAQTSADVVAAVNFARAHRLRLVVKGGGHSYQGTSNAADSLLV
                     WTRGMRAIALHDAFVGAGCTGPPQPAVTVEAGALWGPVFDAVTTRGGRYVQGGGCLTV
                     GVAGLVQSGGFGSFSKGFGLAAASLLQAEVVTADGMLRIANACTNPELFWGLKGGGGG
                     SLGVVTRLTLRTHVLPASIGAVNMTIQANSDAAYRRLIGLVTEHCRDRLMNPHWGEQI
                     VLARDNVLRIAMVFQGLDRGAASAVWQPFIDAVSAAPREFQMTSAPFIVALPARRFWE
                     PGFLKLLPGVVVGDNRPGAPPGNIIWSGDQGQVGQVLHGYQSAWLPAALLQPDRRESL
                     GDALFAATRHWQVALHLNKGLAGAPDAAIAAARDTAMNPAVLDAFALAIVAAGQAPAY
                     PGVAGHEPDAPRARQRSQAVADAMAALRGLMPNPGSYLAESDFFEPDWPRAFWGANLA
                     RLQAVKRRYDPDGLFFAHHSVGSERWSADGFTPLR"
     gene            complement(3571..>4830)
                     /locus_tag="VFA35_10320"
     CDS             complement(3571..>4830)
                     /locus_tag="VFA35_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56611.1"
                     /translation="LLEELGVRRSERGQVVYDERMLCHSPQERLHIDGFWHDGLLPPI
                     AALPAAERATTLAQYRRFGAAVDAAGAGGAFALPTARSAWSARLDALDAVSFAAWLDA
                     QGIDAPALRWYLDYCCRDDYGAGAAQVSAWAGLHYFASRHGFHAPGDANDERDTVLTW
                     PEGNAWLARRLAVPLRERTHTGCIALRVVEGRDAVEVDVWNERAHRAERWRAPQVVLA
                     LSLFVAARLLDAPPPALRAAVALMPHAPWLVGNLHLADALDDPPGAPLSWDNVVYDAG
                     DANPTLGYVDAMHQSTRSLPGPTVLTAYWALGGNTPAQLAQSRARLLERPWSSWAAAL
                     VAKLATVHADLPAKLKQVDLMRYGHAMAVPAPGLRSGAALRALAAPQSRIQFAHGDLS
                     AYSVFEEAIYHGTRAGAAAAGRLRRRA"
BASE COUNT          589 a         1810 c         1814 g          617 t
ORIGIN      
        1 gcatcgcgtt cgagcgcgcc gagctgcgcc gccagttgac gcaggtcaac gccgcggcgc
       61 gcctgccgaa cctgctgcgc ggcgtgatcg gcggcaacct cggccaggcg ctgttcggcg
      121 ccgccgggcc ggcgggccag ggcggctggg tcgcgctggc cctgtcgctg ctgcggcgct
      181 accgggtcgc ggcggccctg ctcggcagtg cagcgccgat gctgcgcggg cgtggcggct
      241 ggcggcgggt cgtgcgcgtg ggcgtgctgg ccgctgcggc gtacttcggc tggcgcctcg
      301 tgcaggggca cgagcggcag cgctgaacgc tcacggccgt tcagggatgg ccccggcttc
      361 ggctttcgtc gagcgccgtg cgcggctcgc tgcggcgctg cgcgccgccg gcggcggcgt
      421 ggccgtgatc ccgaccgcgc cggagcgcca gcgcaacagc gacaacgacc acccgttccg
      481 ccacgacagc tcgttccact acctgaccgg cttcgacgag cccggcgcct ggctggtgct
      541 gcaggcaagc ggcgaaacgg cgctcggctg ccacccgaac gacctggaga cggcgatctg
      601 gcacggccac cggctcggtg tcgaggcagg gccggccgcg ctcggggtcg acgccgcgtt
      661 cgatgtcgac acgctcgacg ccgtgatgct cgcgcgcctc gccggcgcgc cggcggtgtg
      721 gttcccgttc gacgccggcc ccgaggtcgc gcgccgcatc gacggttggc tcgccacgct
      781 gcgcggccgc gcgcgcagcg gcgtcgaggc gccggcggtg cagcgcgacc tcgccccgct
      841 cgtcggcgaa atgcgcctcg tcaaagacgc cggcgagctc gcaacgctgc gccgcgccgc
      901 gcagatcagc gccgatgcgc acgcgcgcgc gatgcgcttt tgcggcgcgc gctttcgcgc
      961 cgcgccggat ggcacgctgc gcgagtacga gatcgaggcc gagctgctgc acgagttccg
     1021 ccgccgcggc gcgcagagcc cggcctatcc gtcgatcgtc gcggcaggcg cgaacgcctg
     1081 cgtgctgcac tacgccgcgg gcgacgccga gttgcgcgcc ggcgagctgt gcctgatcga
     1141 cgccggctgc gaactcgacg gctacgccag cgacatcacc cgcacgttcc cggccaacgg
     1201 ccgcttcacg ccggcgcagc gcgctctcta cgagatcgtg ttcgcggcgc agcaggccgc
     1261 cgtcgacgcg acgcgccccg gcgcgcgcca gcgcgatgcg caccacgccg cggtgcgcgt
     1321 gctcacgcaa ggcatgctcg acagcgggct gctcgaccgc aacgccgtcg gcaacgtcga
     1381 tgacgcgatt cgctcgtgcg cctaccgcca gttctacatg cacggcaccg gccactggct
     1441 cgggcgcgac gtgcacgacg tgggcgagta cctcgcgctc gacgagccgc cggggccgca
     1501 gatcgacggc atgggccgcg cggtcgtgat gaagccgtcg cgcgtgctcg tgccgggcat
     1561 ggtcgtgacg ctcgagccgg gcctctacgt gcgcccggcg gtcggcgtgc cggagcgctt
     1621 ctggcacatc ggcatccgca tcgaggacga cgcggtcgtg accgacaccg gctgcgagct
     1681 gatcagccgc ggcgtgccgg tggccgcggc cgagatcgag gccttgatgc gcgcgtagag
     1741 cgcgcgcctg ccgaggcatg gaccgccgcc gactcctgcg ctccgccgcc gcgccgctgc
     1801 tggccggcct gcaacccggc gcgctgacga atgcgctcgc ggcacccggc ccgctgccgc
     1861 gcgtgcgccc cggcgacgcg gcctggccgt ccgaagcgcg ctgggccgag ctcggcgagc
     1921 gcgtcggcgg ccggctgctc gcgctggccg acccgttcaa agcctgccgc gacgcgccgg
     1981 ccggcgccgc gtgcgccgaa ctcttcgggt cgctgaagaa cccgcacgtc atcggcgatt
     2041 cgcccgcgct gacgcagacg ctgggctggg tcgacgcctg gacctcgcgg accagcgcct
     2101 acgccgtggc cgcgcagacg agcgccgatg tcgtcgccgc ggtgaacttc gcgcgcgcgc
     2161 accggcttcg gctggtcgtc aagggcggcg gccacagcta ccagggcacc tcgaacgcgg
     2221 ccgactcgct gctcgtgtgg acgcgaggca tgcgcgcgat cgcgctgcac gacgcgttcg
     2281 tcggcgccgg ctgcaccggg ccgccgcagc cggccgtgac ggtcgaggcc ggcgcgctct
     2341 gggggccggt cttcgatgcg gtcacgaccc ggggcgggcg ctacgtgcaa ggcggcggct
     2401 gcctgaccgt gggggtggcc gggctggtcc agagcggcgg cttcggcagc ttctcgaaag
     2461 gcttcgggct ggcggcggcg agcctgctgc aggccgaggt cgtgaccgcc gacggcatgc
     2521 tgcgcatcgc caacgcctgc acgaacccgg agctgttctg ggggctcaag ggcggcggtg
     2581 gcggcagcct tggcgtcgtg acccggctga ccttgcgcac gcatgtgctc ccggcgtcga
     2641 tcggcgcggt caacatgacg atccaggcga attccgacgc cgcgtaccgg cgcctgatcg
     2701 ggctcgtcac cgaacactgc cgcgaccgcc tgatgaaccc gcactggggc gagcagatcg
     2761 tgctggcgcg cgacaacgtg ctgcggatcg cgatggtgtt ccaaggcctt gatcgcggcg
     2821 cggcctcggc cgtgtggcag cccttcatcg acgcggtcag cgcggcgccg cgcgagttcc
     2881 agatgacatc cgcgccgttc atcgtggcgc tgccggcgcg ccgcttctgg gagccgggct
     2941 tcctgaagct gctgcccggc gtggtcgtcg gcgacaaccg gcccggtgcg ccgcccggca
     3001 acatcatctg gtcgggcgac caaggccagg tcggccaggt gctgcacggc taccagtcgg
     3061 cgtggctgcc ggccgcgctg ctgcagccgg accggcgcga gtcgctgggc gacgcgctgt
     3121 tcgcggcgac gcgccactgg caggtcgcgc tgcacctcaa caagggcctg gccggcgcgc
     3181 cggacgcggc gatcgcggcg gcgcgcgaca cggcgatgaa cccggccgtg ctcgacgcgt
     3241 tcgcgctcgc gatcgtggcg gccgggcaag cgccggccta tcccggcgtg gccggccacg
     3301 aacccgatgc gccgcgcgcc cgccagcgct cccaggccgt cgccgacgcg atggccgcgt
     3361 tgcgcggcct catgccgaac ccgggctcgt acctcgccga gagcgacttc ttcgagcccg
     3421 actggccgcg cgcgttctgg ggcgcgaacc tcgcgcgcct gcaggccgtg aagcggcgtt
     3481 acgaccccga cggcctgttc ttcgcgcacc acagcgtcgg cagcgagcgc tggagcgcgg
     3541 acggcttcac accgttgcgc tgactcacgc tcacgcgcgc cgccgaagcc gcccggcggc
     3601 ggctgcgccg gcgcgcgtgc cgtggtagat cgcctcctcg aacaccgagt aggccgacag
     3661 gtcaccgtgc gcgaactgga tgcgggactg cggcgccgcc agcgcacgca acgccgcgcc
     3721 gctgcgcagc cccggcgccg gcacggccat cgcgtggccg tagcgcatca gatcgacctg
     3781 cttcagcttg gcgggcaggt cggcgtggac cgtcgccagc ttcgcgacca gcgccgcggc
     3841 ccagctcgac cagggccgct ccagcagccg cgcgcgcgat tgcgcgagct gcgcgggcgt
     3901 gttgccgccg agtgcccagt acgcggtcag cacggtcggc ccgggcaggc tgcgcgtgct
     3961 ctggtgcatc gcgtcgacat agcccagcgt cgggttcgcg tcgccggcgt cgtagacgac
     4021 gttgtcccac gacagcggcg cgccgggcgg gtcgtcgagc gcgtcggcga ggtgcaggtt
     4081 gccgaccagc cagggcgcat gcggcatcaa cgcgaccgcc gcgcgcaacg ccggcggcgg
     4141 cgcgtcgagc agccgcgcgg cgacgaacag cgacagcgcg agcacgacct gcggcgcgcg
     4201 ccaacgctcg gcgcggtgcg cgcgctcgtt ccacacgtcg acctcgacgg cgtcgcggcc
     4261 ctcgacgacg cgcaatgcga tgcaccccgt gtgggtgcgt tcgcgcagcg gcacggcgag
     4321 ccggcgcgcg agccaggcgt tgccctcggg ccaggtcaac accgtgtcgc gctcatcgtt
     4381 cgcgtcgccc ggcgcatgaa agccgtgccg gctcgcgaag tagtgcagcc cggcccaggc
     4441 cgagacctgc gcggcgccgg cgccgtagtc gtcgcggcag cagtagtcga ggtaccagcg
     4501 cagcgcgggc gcatcgatgc cctgcgcgtc gagccaggcg gcgaagctga cggcatcgag
     4561 cgcgtcgagc cgcgcgctcc acgcgctgcg cgcggtcggc agcgcgaacg cgccgccggc
     4621 gcccgcggcg tcgaccgcgg cgccgaaccg ccggtactgc gcgagcgtgg tcgcacgctc
     4681 ggccgcgggc agcgcggcga tcggcggtag cagaccgtcg tgccagaagc cgtcgatgtg
     4741 caagcgctcc tgcggcgagt ggcacagcat gcgctcgtcg tacacgacct ggccgcgctc
     4801 gctgcgccgc acgccgagtt cctcgagcaa
//
LOCUS       DASRDG010000617         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_666788, whole genome shotgun sequence.
ACCESSION   DASRDG010000617 DASRDG010000000
VERSION     DASRDG010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666788"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFA35_10325"
     CDS             <1..221
                     /locus_tag="VFA35_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HZT56612.1"
                     /translation="NLPPDAPDAMVFHAGTALQGHDTVTAGGRVLCVTALGDSVKLAQ
                     QRAYEVLRGIHFDGAQYRRDIGYRAIKR"
     gene            218..1159
                     /gene="hemF"
                     /locus_tag="VFA35_10330"
     CDS             218..1159
                     /gene="hemF"
                     /locus_tag="VFA35_10330"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HZT56613.1"
                     /translation="MSTDTRAVRAYLLGLQDRIVAAIEAEDGQSFITDAWQRDPGGKL
                     EGEGLSRLLEGGAVIERGGCGFSHVKGKALPPSATQHRPELAGAPFEAMGVSLVLHPR
                     NPFVPTVHMNVRMLAAFPVDPAAGGTPVTWFGGGMDLTPYYGFEADAAHFHRVNRAAL
                     EPFGADLYPRFKQWCDDYFFLKHRNEPRGIGGVFFDDFAEAGFERSFAMLRAVGDALL
                     PAYLPILQRHKNDAYGERERAFQTYRRGRYVEFNLVFDRGTLFGLQSGGRTESILMSM
                     PPVVTWRYDWQPEPGTPEARLYSEFLRPRNWADWTPD"
     gene            1156..1776
                     /gene="nadD"
                     /locus_tag="VFA35_10335"
     CDS             1156..1776
                     /gene="nadD"
                     /locus_tag="VFA35_10335"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZT56614.1"
                     /translation="MNIGIFGGSFDPVHNAHVALARQALAELALDELIWLPAGQPWQK
                     RRVLAPAADREAMVRLAIEGEPRFTLSRIETSRSGPSYTIDTVCELRAQRPGVSWHLV
                     IGQDQYAAFHTWRGWQELLGLVTLAIANRPGASLAADPQVLRVPHAAVALPMMDISST
                     DIRARLAHRQPIDDLVPAPVARYIARHHLYLGPTGPAPAQELNGHS"
     gene            1763..2431
                     /gene="rsfS"
                     /locus_tag="VFA35_10340"
     CDS             1763..2431
                     /gene="rsfS"
                     /locus_tag="VFA35_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZT56615.1"
                     /translation="MDIRKLQRVIVDALEDVKAQNIVVFNTEHLSALFERVIIASGTS
                     NRQTKSLASSVREAVKTAGYPVPRTEGEGNGEWIIIDCGAAVVHVMQPTIRDYYHLEE
                     IWGDKPVNMKIAAGATKLVKAAEPAAPAKKVAAKKVVAKKAPAAKAPAKKAAAKKAPA
                     KSIPVVKKTLVVGRPAAKKKPAAVVGRGVTRTVGVKSTAAQQAPAKKAAAKKASARKT
                     SGRA"
     gene            2501..2968
                     /gene="rlmH"
                     /locus_tag="VFA35_10345"
     CDS             2501..2968
                     /gene="rlmH"
                     /locus_tag="VFA35_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZT56616.1"
                     /translation="MRLLVVAVGQRQPAWAQTAYAEFEKRFPPEMRLELHAVKAETRG
                     QKSAEQLMAAEGQRLQAALPRGVRRVVLDERGTRVTTLQLADRMKAWQHDGRDVAFMI
                     GGPDGLDASLKATADETLRLSELTLPHAFVRVLLAEALYRAWTVTVNHPYHRE"
     gene            2975..3586
                     /locus_tag="VFA35_10350"
     CDS             2975..3586
                     /locus_tag="VFA35_10350"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZT56617.1"
                     /translation="MSDTAPFIYLASQSPRRRQLLDQLGVRHELLLADADEDAEALEA
                     ERAGELPAAYVERVTRAKLQAAKARRRRRGLPDAPVLCADTTVALGRRILGKPADAAA
                     AVATLMLLSGRTHRVITAVAVAAGRRTGFAVSSSRVRVAPIPAAAIAAYVAGGEPFGK
                     AGAYAIQSSFSGWIAGIDGSYSGIMGLPLFETAELLRRAGLAI"
     gene            3633..>4765
                     /locus_tag="VFA35_10355"
     CDS             3633..>4765
                     /locus_tag="VFA35_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZT56618.1"
                     /translation="MQDILINWTPQETRVAIVENGAVQELHVERALERGLVGNIYAGK
                     VVRVLPGMQSAFIDIGLERAAFLHVADVQVNGVVPRGSYGAASRGEHANNGAPPTPIE
                     RLVFEGQTLTVQVIKDPIGTKGARLSTQISIAGRLLVFLPQDDHIGISQKIGSHELRE
                     QLRARMGVLAQVPEGSGPQGGFILRTNAEEATDAELSDDIRYLRKTWAAVRAKGFKSP
                     PGALLHQDLSLVERVLRDLTHDGTQAVRIDSRMQFELLHKFGQEFTPTSVAKLEHYAG
                     ERPIFDQFNIDEEIARALMRRVDLKSGGYLIIDQTEALTTIDVNTGGFVGARNFDDTI
                     FKTNLEAAQSLARQLRLRNLGGIIIIDFIDMAREDHQASVLGEL"
BASE COUNT          770 a         1661 c         1655 g          679 t
ORIGIN      
        1 ccaacctgcc gcccgacgcg ccggacgcga tggtgtttca tgccggcacg gcgctgcagg
       61 gccacgacac cgtgaccgcc ggtgggcggg tgctgtgcgt gacggcgctc ggcgactcgg
      121 tcaagctcgc gcagcagcgc gcctacgagg tgctgcgcgg catccacttc gatggcgcgc
      181 agtaccggcg cgacatcggg taccgggcga tcaagcgttg agcaccgaca cccgcgcggt
      241 ccgcgcgtac ctgctcggct tgcaggaccg catcgtcgcg gcgatcgagg ccgaggacgg
      301 ccagtcgttc atcaccgacg cctggcagcg cgacccgggc ggcaagctcg aaggcgaagg
      361 cctgtcgcgc ctgctcgaag gcggcgcggt gatcgagcgc ggcggctgcg gcttctcgca
      421 cgtcaagggc aaggcgctgc cgccgtcggc gacgcagcat cgcccggagt tggccggcgc
      481 gcccttcgag gcgatgggcg tgtcgctggt gctgcacccg cgcaatccgt tcgtgccgac
      541 cgtgcacatg aacgtgcgca tgctcgcggc cttccctgtt gatcccgccg ccggcggcac
      601 gccggtgacc tggttcggcg gcggcatgga cctgacgccg tactacggct tcgaagccga
      661 tgccgcgcac ttccaccgcg tcaaccgcgc cgcgctcgag cccttcggcg ccgacctgta
      721 cccgcgcttc aagcaatggt gcgacgacta cttcttcctg aagcaccgca acgagccgcg
      781 cggcatcggc ggtgtgttct tcgacgactt cgctgaagca ggcttcgagc gcagcttcgc
      841 gatgctgcgc gccgtcggcg atgctttgct accggcctac ttgccgatcc tgcagcgcca
      901 caagaacgac gcttacggcg aacgcgaacg cgcgttccag acctaccggc gcggccgcta
      961 cgtcgagttc aacctcgtgt tcgaccgcgg cacgctgttc ggcctgcagt cgggcgggcg
     1021 caccgagagc atcctgatgt cgatgccgcc ggtcgtgacc tggcgctacg actggcagcc
     1081 cgagccgggc acgccggagg cgcggctgta cagcgaattc ctgcgcccgc gcaattgggc
     1141 cgattggact cccgattgaa cattggcatc ttcggcggca gctttgatcc ggtgcacaac
     1201 gcgcacgtcg cgctggcgcg gcaggcgctg gccgagctcg cgctcgacga gctgatctgg
     1261 ctgcccgccg gccagccgtg gcaaaagcgt cgcgtgctcg cgcccgcggc cgatcgcgag
     1321 gcgatggttc ggctcgcgat cgagggcgaa ccgcgcttca cgctgtcgcg catcgagacc
     1381 tcgcgcagcg gcccgagcta caccatcgac accgtgtgcg aattgcgcgc gcagcgcccc
     1441 ggcgtgagct ggcacctcgt gatcgggcag gaccagtacg ccgccttcca cacctggcgc
     1501 ggctggcagg agcttctggg cctcgtcacg ttggcgatcg cgaaccgtcc gggcgcctcg
     1561 ctcgcggccg acccgcaggt gttgcgcgtg ccgcacgcag ccgtcgcgct gccgatgatg
     1621 gacatctcgt cgaccgacat ccgcgcgcgc ctcgcgcacc ggcagccaat cgacgatctg
     1681 gtgcccgccc cggtggcacg ctatattgcc cgccaccacc tttacctggg tccgaccgga
     1741 cccgcccccg ctcaggagct gaatggacat tcgtaaactg caacgcgtca tcgtcgatgc
     1801 actggaagac gtcaaggccc agaacatcgt cgtgttcaac accgagcacc tgtcggccct
     1861 gttcgagcgc gtgatcatcg cctcgggcac gagcaaccgg cagaccaagt cgctggcgtc
     1921 cagcgtgcgc gaggcagtca agacggcggg ctacccggtg ccgcgcaccg aaggcgaggg
     1981 caacggcgaa tggatcatca tcgattgcgg cgccgccgtg gtgcacgtga tgcagccgac
     2041 gattcgcgac tactaccacc tcgaagagat ctggggcgac aagccggtca acatgaagat
     2101 cgcggccggc gccaccaagc tggtgaaggc ggccgagccg gctgcgccgg cgaagaaggt
     2161 ggccgcgaag aaggtcgttg ccaagaaggc cccggccgcg aaggcgcccg cgaagaaggc
     2221 cgcggcgaag aaggctcccg cgaagagcat tccggtcgtc aagaagacgc tggtcgtcgg
     2281 caggccggcg gcaaagaaga agccggcggc agtggtcggg cgaggcgtga cgcgcaccgt
     2341 cggcgtgaag tcaacggcgg cccaacaggc gccggccaag aaggccgcgg cgaagaaggc
     2401 ctcagcccgg aagacctccg gccgcgcatg agcgcgtcgg gccgccccga gcgcgaagct
     2461 cccctcgcgc aggggtggcg cgcagcgcct ggggtacatc attaggctct tggtcgtcgc
     2521 ggtcggccag cgccagccgg cctgggcgca gacggcctat gccgagttcg agaagcgctt
     2581 tccgcccgag atgcggctcg aactgcatgc ggtgaaggcc gagacccgcg gccagaagag
     2641 cgccgagcag ctgatggccg cggaaggcca gcgcctgcag gcggcgctgc cgcgcggcgt
     2701 gcgccgggtc gtgctcgacg aacgcggcac ccgggtcacc acgctgcaac tggccgatcg
     2761 gatgaaggcg tggcagcacg atggccgcga cgtcgccttc atgatcggcg ggcctgacgg
     2821 gctcgatgcg tcgttgaagg cgacggccga cgaaaccttg cgcctttccg agctgacctt
     2881 gccgcacgcg ttcgtgcgcg tgctgctcgc cgaggcgctc taccgcgcgt ggaccgttac
     2941 ggtcaatcat ccctaccacc gggagtgact cgccgtgagt gacaccgcac cgttcatcta
     3001 cctcgcatcg caaagcccgc gccgccgcca gctgctcgac cagctcggcg tgcgccacga
     3061 gttgctgctg gccgatgccg acgaagacgc cgaggcgctg gaggccgagc gcgccggcga
     3121 gttgccggcc gcctatgtcg agcgcgtcac gcgcgccaag ctgcaggcgg cgaaggcacg
     3181 ccggcggcgc cgcgggctgc ccgacgcgcc ggtcctgtgc gccgacacca cggtggcgct
     3241 gggccggcgc atcctcggca agcctgccga cgcggccgca gcggtcgcga cgctgatgct
     3301 gctgtcgggg cgcacccacc gcgtcatcac cgcggtggcc gtcgccgccg ggcggcgcac
     3361 cgggttcgcc gtcagcagct cgcgggttcg ggttgcaccg atcccggccg cggcgatcgc
     3421 ggcctatgtg gccggcggcg agcccttcgg caaggccggg gcctacgcga tccagagcag
     3481 cttctcgggc tggattgccg gcatcgacgg cagctactcg ggcatcatgg ggctgccgct
     3541 gttcgagacc gccgagttgc tgcgccgcgc gggcttggca atctgaagga cggcttaccg
     3601 atgcaaacaa gaacagcagg gacagcaacg acatgcagga catcctgatc aattggacgc
     3661 cgcaggagac gcgcgtcgcg atcgtcgaga acggcgcggt tcaggaactg cacgtcgaac
     3721 gcgcgctgga gcgcggcctg gtcggcaaca tctacgccgg caaggtggtg cgcgtgctgc
     3781 cgggcatgca gtcggccttc atcgacatcg gcctggagcg cgccgcgttc ctgcacgtgg
     3841 ccgatgtgca ggtgaacggc gtggtaccca ggggcagcta cggcgccgcg tcgcgcggcg
     3901 agcatgccaa caacggcgcg ccacccacgc cgatcgagcg cctcgtgttc gagggccaga
     3961 cgctgaccgt gcaggtcatc aaggacccga tcggcaccaa gggcgcgcgg ctgtcgacgc
     4021 agatcagcat cgccgggcgg ctgctcgtct tcctgccgca ggacgatcac atcggcatct
     4081 cgcagaagat cggttcgcac gagctgcgcg agcagctgcg cgcgcgcatg ggcgtgctcg
     4141 cgcaggtgcc cgaagggtcg gggccgcagg gcggcttcat cctgcgcacg aacgccgagg
     4201 aggcgacgga cgccgaactc tccgacgaca tccgctacct gcgcaagacc tgggccgcgg
     4261 tgcgcgccaa gggcttcaag tcgccgccgg gcgctctgct gcaccaggac ctcagcctgg
     4321 tcgagcgcgt gctgcgcgac ctcacgcacg acggcacgca ggcggtgcgg atcgactcgc
     4381 gcatgcagtt cgagctgttg cacaagtttg gccaggagtt cacgccgact tcggtcgcca
     4441 agctcgagca ctacgcgggc gagcggccga tcttcgacca gttcaacatc gacgaggaga
     4501 tcgcgcgggc gctgatgcgc cgcgtcgatc tcaagtcggg cggctacctg atcatcgacc
     4561 agaccgaggc gctcaccacg atcgacgtca acaccggcgg cttcgtcggc gcgcgcaact
     4621 tcgacgacac gatcttcaag accaatctcg aggcggcaca gtcgctggcc cgacagctgc
     4681 gtctgcgcaa cctcggcggc atcatcatta tcgacttcat cgacatggcg cgcgaggacc
     4741 accaggcctc ggtgctcggc gagct
//
LOCUS       DASRDG010000618         4858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_456311, whole genome shotgun sequence.
ACCESSION   DASRDG010000618 DASRDG010000000
VERSION     DASRDG010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456311"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VFA35_10360"
     CDS             complement(<1..701)
                     /locus_tag="VFA35_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZT56619.1"
                     /translation="MDHLSSMDASFLHFETPETPMHVASLSLLSLPEGYKGDYFEDVK
                     AMIGKRMHLATVLTRKLAQMPFELAEPVWIDDDDIDLDYHVRSLTLRRPGTMEQLEAL
                     VARLHSTLLDRSRPLWEMYVIEGLQDGQVAFYSKAHHSGVDGKAGTEMAKVLYDISPQ
                     MREVTPPRRKNAQGSSGYQLGVAELLQAAVSNSAAQYRKLAALLPTAAKALTAAGKVL
                     ASQRTAKGERPLNLG"
     gene            complement(760..2106)
                     /locus_tag="VFA35_10365"
     CDS             complement(760..2106)
                     /locus_tag="VFA35_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZT56620.1"
                     /translation="MHLHFVRLAVATAAAGLFSGAACAQAIDAASLTDLDKRATALQP
                     KMVAWRRDIHEHPELSGQELRTAKLVADHLRKLGLEVKTGVGGTGVVGLLKGGKPGKV
                     VALRADMDALPVKEDTGLPFASKVIAKNFGKDAPVAHACGHDGHTAILMGVAELLAGM
                     REQIAGSVKFIFQPAEEGRSEEPKDPNAPFGAKWMIADGALDNPKVDAMFGLHLGPGF
                     PGWSVGTIGYRIGPFLAGSDNFRIKVTGRQTHGSRPWDGIDPIVASAQIVTGLQTIVS
                     RQLDISVVPTVVTIGAINGGLRENIVPDSVDMLGNLRSFNNELRADAMKRITTTATSI
                     AAASGAKAEVVFPPTFWPPTVNPAALSEQAVPVLQAVAGGKAVIAPLIPGSEDFADFQ
                     LKVPGFFFLLGAPPKGKTPANSATNHSPKFDFDEDAMPVGAKALAALALDQLARPK"
     gene            complement(2157..3857)
                     /locus_tag="VFA35_10370"
     CDS             complement(2157..3857)
                     /locus_tag="VFA35_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZT56621.1"
                     /translation="MNDMPWLASYPDGMRWDLAIEPAPVQKILDDTVAKWPDRPAVEF
                     MGKRISYRELGSLTDRMAKGLQQLGVKPGVHVGIYLPNSPHTVIAFFGILKAGGTVVN
                     YSPLDAAKVLEHKIEDSQTDFLVTLDLAALYPNMAGMFGKTRLKKLIVGNMGEMTPAP
                     DAVNAQMKAGKQLADVTWDDQHVKFAALLDNDGQYTRYPMANPADAIAVLQYTGGTTG
                     WPKGAMLTHANLSAATAQYVESTRGKPPLLEEGKERFLCVLPPFHIYALTVNMLFGLK
                     MGAELIQHGRFDPKAALEDIANKKITAFCGVPTMFTALIDHPDTPKYNLRTLKYCGSG
                     GAPLPVEVGQRYTEITGAPISEGWGMTETSPTGTFSPTGKSKAGSCGIPLPNIVIKLL
                     SLEDPTKYVALGEKGEMCISGPNVMKGYWKKPEATAEEMTFDGLFRTGDVAYMDDDGY
                     VFIVDRCKDMLLCSGFNVYPRVLEEAIYLHPSVGEVCVIGIPDAYRGQSPKAFVALKA
                     GAQPFTLKELQAFLKDKLGKHEMVQELEIRDALPKTAVGKLSKKDLVDEEGRKRAAQA
                     "
     gene            complement(4001..4786)
                     /locus_tag="VFA35_10375"
     CDS             complement(4001..4786)
                     /locus_tag="VFA35_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT56622.1"
                     /translation="MTETDPSAPPSRRLLMLEARAIPELSAYFSMAPALRLAPAGDGH
                     PVLVLPGLAASDTSTRPLRAFLRDRGYHAHGWKLGNNFGLRPGVEAQMDERLAELHAR
                     HGRKVSLIGWSLGGIFARELARRAPAKVRGVITLGSPFAGAPRASNAWRLYESVSESS
                     VDDWAEREVAKEPPPVPATAIFSRSDGIVAWQGCLEREGPMSENIEVEGSHCGLGHNP
                     IVLYAIADRLAQAEGQWKPFDRSGMRRFVYPDPQRDRLSAAMR"
BASE COUNT          708 a         1609 c         1682 g          859 t
ORIGIN      
        1 atgcccaggt tcagcggccg ctcgcccttg gcggtgcgct ggctggccag caccttgccg
       61 gccgcggtga gggccttggc cgcggtgggc agcagcgcgg cgagcttgcg gtactgcgcc
      121 gccgagttgg acaccgccgc ctgcagcagt tcggccacgc caagctggta gccgctgctg
      181 ccctgggcgt tcttgcgccg cggcggcgtc acctcgcgca tctgcggcga gatgtcgtag
      241 agcaccttgg ccatctcggt gccggccttg ccgtcgacgc cgctgtggtg ggccttgctg
      301 tagaaggcga cctgaccgtc ctgcaggccc tcaatgacgt acatctccca cagcggccgg
      361 ctgcggtcga gcagggtcga atgcagccgt gcgacgagtg cctccaactg ctccatcgtg
      421 ccgggccggc gcagcgtcag gctgcgcacg tggtagtcca ggtcgatgtc gtcgtcgtcg
      481 atccacaccg gctcggccag ctcgaacggc atctgcgcca gcttgcgcgt cagcaccgtc
      541 gccaggtgca tgcgtttgcc gatcatggcc ttcacgtcct cgaagtagtc gcccttgtag
      601 ccctcgggca agctcagcaa cgacaggctg gccacatgca tcggtgtctc gggcgtctcg
      661 aagtgcagga acgaggcgtc catgctgctg aggtggtcca ttaggaggcc tttcagttga
      721 attcgtttga cgggcggctc gatgggctgc gttgtgccgc tacttcggcc gcgccagctg
      781 gtcgagcgcg agcgccgcga gggccttcgc acccacgggc atcgcgtcct cgtcgaagtc
      841 gaacttcggc gaatggttgg tcgccgagtt ggcaggcgtc ttgcctttcg gcggcgcgcc
      901 gagaaggaag aagaaaccgg gcaccttgag ctggaagtcg gcgaagtcct ccgagccggg
      961 gatcagcgga gcgatcaccg ccttgccgcc ggcgacggcc tgcagcaccg gcaccgcctg
     1021 ctcggacagc gcggccgggt tcacggtcgg cggccagaac gtgggcggga agaccacctc
     1081 ggccttcgca ccgctggccg cggcgatcga cgtggcggtg gtggtgatgc gcttcatcgc
     1141 atcggcacgc agctcgttgt tgaaggagcg caggttgccg agcatgtcga cgctgtcggg
     1201 gacgatgttc tcgcgcaggc caccgttgat cgcgccgatg gtcaccaccg tgggcaccac
     1261 ggaaatgtcg agctggcggc tgacgatggt ctgcaggccg gtgacgatct gcgccgacgc
     1321 gacgatcggg tcgatgccgt cccaaggccg cgagccatgc gtctggcgcc cggtgacctt
     1381 gatgcggaag ttgtcggagc cggccaggaa cgggccgatg cggtagccga tcgtgccgac
     1441 ggaccagccg gggaaccccg gtccgaggtg caggccgaac atcgcgtcga ccttcgggtt
     1501 gtcgagcgcg ccgtcggcga tcatccactt cgcgccgaac ggcgcgttcg gatccttcgg
     1561 ctcctcggag cgcccctcct cggccggctg gaagatgaac ttgacgctgc cggcgatctg
     1621 ctcgcgcatg ccggcgagca actcggccac gcccatcagg atcgcggtgt ggccgtcgtg
     1681 gccgcaggcg tgcgcgaccg gcgcgtcctt gccgaagttc ttcgcgatca ccttcgacgc
     1741 gaacggcagg ccggtgtctt ccttgaccgg cagcgcatcc atgtcggcgc gcagcgcgac
     1801 caccttgccg ggcttgccgc ccttgagcag gccgacgacg ccggtgccgc cgacgccggt
     1861 cttgacctcc agcccgagct tgcgcaggtg atcggcgacc aacttcgcgg tgcgcagctc
     1921 ctgacccgac aactcggggt gctcgtggat atcgcgccgc cacgcgacca tcttcggctg
     1981 caaggcggtg gcgcgcttgt cgaggtcggt cagcgaggcg gcatcgatgg cctgcgcgca
     2041 cgcagcaccg gagaacaggc ctgccgcagc agtcgcgacg gcgaggcgaa cgaaatgcaa
     2101 gtgcatggga atccttacgg ggtctcgggg gcgggctcga tgcccggtcg aagggctcag
     2161 gcctgcgccg cgcgcttgcg cccttcctcg tcgaccagat ccttcttcga cagcttgccc
     2221 accgccgtct tgggcagcgc gtcgcggatc tccagctcct ggaccatctc gtgcttgccg
     2281 agcttgtcct tcaggaaggc ctgcagctcc ttcagcgtga acggctgcgc gccggccttg
     2341 agcgcgacga aagccttggg cgactgaccg cggtaggcat ccgggatgcc gatcacgcag
     2401 acctcgccga ccgacgggtg cagatagatc gcctcctcga gcacgcgcgg gtagacgttg
     2461 aagccgctgc acagcagcat gtccttgcag cggtcgacga tgaagacata accgtcgtcg
     2521 tccatgtagg ccacgtcgcc ggtgcggaac aggccgtcga aggtcatctc ctcggcggtc
     2581 gcttccggct tcttccagta gcccttcatc acgttcgggc cgctgatgca catctcgccc
     2641 ttttcgccga gcgcgacgta cttcgtcggg tcttcgagcg acaacagctt gatcacgatg
     2701 ttgggcagcg ggatgccgca ggagccggcc ttgctcttgc cggtgggcga gaaggtgccg
     2761 gtgggcgagg tctcggtcat gccccagcct tcggagatcg gggcgccggt gatctcggtg
     2821 tagcgctgcc ccacctcgac cggcagcggc gcgccgcccg agccgcagta cttcagcgtg
     2881 cgcaggttgt acttgggcgt gtccggatgg tcgatcagcg cggtgaacat cgtcggcacg
     2941 ccgcagaagg cggtgatctt cttgttcgcg atgtcctcca gcgcggcctt cgggtcgaag
     3001 cgcccatgct ggatcagctc ggcgcccatc ttcaggccga acagcatgtt caccgtcagc
     3061 gcgtagatgt ggaagggcgg cagcacgcag aggaagcgct ccttgccttc ctcgagcaac
     3121 ggcggcttgc cgcgcgtgga ctcgacgtat tgcgcggttg ccgccgagag gttggcgtgc
     3181 gtgagcatcg cgcccttggg ccagccggtc gtgccgccgg tgtactgcag cacggcgatg
     3241 gcgtccgccg gattggccat ggggtagcgc gtgtactggc cgtcgttgtc cagcagcgcg
     3301 gcaaacttga cgtgctggtc gtcccaggtg acatcggcga gctgcttgcc cgccttcatc
     3361 tgcgcgttca ccgcgtcggg cgccggggtc atctcgccca tgttgccgac gatcaacttc
     3421 ttcaaccgcg tcttgccgaa catgccggcc atgttcgggt acagcgccgc caggtccagc
     3481 gtgaccagga agtcggtctg gctgtcctcg atcttgtgtt cgagcacctt ggctgcgtcg
     3541 agcggcgagt agttgaccac cgtgccgccg gccttgagga tgccgaagaa ggcgatcacg
     3601 gtgtgcggcg agttcggcag gtagatgccc acatgcacgc cgggcttgac gcccagttgc
     3661 tgcaggccct tggccatgcg gtcggtcagg ctgcccagct cgcggtagct gatgcgcttg
     3721 cccatgaact cgaccgccgg ccggtccggc cacttcgcga ccgtgtcgtc gaggatcttc
     3781 tgcaccggtg caggttcgat cgccagatcc catcgcatcc catcgggata ggaagccagc
     3841 cacggcatgt cgttcatgct tttgtctcct tcgttgttcg agcttggtca agcgtcgggc
     3901 gcttgcgctg ctcgttgtgt gccgatcttt tcgcgcccgc gccgattcgg aaaggtgcga
     3961 tcaggccagt tcgcggtgat ttcgggactc ggcaaaccgg ctatcgcatc gccgccgaga
     4021 ggcgatcgcg ctgcgggtcc gggtagacga agcggcgcat gccgctgcgg tcgaacggct
     4081 tccactggcc ctcggcttgc gccaggcgat cggcgatcgc gtacagcacg atcgggttgt
     4141 gacccaggcc gcagtgactg ccctcgactt cgatgttctc ggacatcggg ccttcgcgct
     4201 cgagacaacc ttgccaggcg acgatgccgt cgctgcggct gaagatcgcg gtggccggca
     4261 ccggcggcgg ctccttcgcc acttcgcgtt cggcccaatc gtcgacgctg ctctcgctca
     4321 ccgattcata gaggcgccag gcgttgctcg cgcgcggcgc gcccgcgaac gggctgccga
     4381 gcgtgatcac gccgcgcacc ttcgccgggg cccggcgcgc cagctcgcgg gcgaagatgc
     4441 cgccgaggct ccagccgatc aggctcacct tgcgcccgtg gcgcgcatgc agttcggcga
     4501 ggcgctcgtc catctgcgcc tcgacgcccg ggcgcaggcc gaagttgttg ccgagcttcc
     4561 agccatgcgc gtgatagcca cggtcgcgca ggaaggcgcg caaggggcgg gtcgacgtgt
     4621 cgctcgccgc gagccccggc aacacgagca ccgggtgtcc gtcgccggcc ggcgccagac
     4681 gcagcgctgg cgccatcgaa aagtaggcgg acaactccgg gatggcgcgc gcctcgagca
     4741 tcagcagtcg cctggacggt ggcgcggacg ggtcggtttc ggtcatgaag gtcggcaatg
     4801 caatgggtgg agaaggagaa cacggtgtgc gcgaggacct cgcgagcctc ggaagggc
//
LOCUS       DASRDG010000619         5053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_899422, whole genome shotgun sequence.
ACCESSION   DASRDG010000619 DASRDG010000000
VERSION     DASRDG010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899422"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFA35_10380"
     CDS             <1..261
                     /locus_tag="VFA35_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZT56623.1"
                     /translation="GPAWWSDAAPMAGFGLPLVVPGIVGGVGGGGESTAMTLEPPRGA
                     DGGLPPIAPPPITAPVPEPQTWALMLAGLAAGALVRRRVARR"
     gene            complement(279..3284)
                     /gene="uvrA"
                     /locus_tag="VFA35_10385"
     CDS             complement(279..3284)
                     /gene="uvrA"
                     /locus_tag="VFA35_10385"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZT56624.1"
                     /translation="MPDPADSSAARASPPEATTIRVQPAEAANAIRVRGARTHNLKNI
                     DLDIPRNQLVVITGLSGSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQLMDKPDVDV
                     IEGLSPAISIEQKATSHNPRSTVGTVTEIHDYLRLLYARAGTPYCPDHDLPLQAQSVS
                     QMVDAVLALPEGTRLMIVAPVVRDRKGEFTELFAEMQAQGYIRFRVDGQTCEAADLPK
                     LKKAEKHDIDVVIDRVKVVAGLQQRLAESFEAALRIADGRAIALEMDTDKAHLFSSKF
                     ACPVCSYSLSELEPRLFSFNSPVGACPTCDGLGHTTVFDPERVVAFPSLSLASGAVKG
                     WDRRNGYTFSLLESVAKHYAFDIDAAFEELPKSAQKVLLHGSGATDIEFVYEADGPRG
                     KQRMVKRSHPFEGILPNLERRFRETDSAAVREDLMRYQSAKPCPDCNGTRLRREARHV
                     FLVDATSAAKEPTERPRCEPTERPRREPIYRVEHFTLRECLAYFESLTLQGAKAEIAD
                     KVVREIRSRLKFLNDVGLNYLSLDRNADSLSGGESQRIRLASQIGSGLTGVMYVLDEP
                     SIGLHQRDNERLIGTLRHLRDIGNSVLVVEHDEDAIRAADHVIDMGPGAGVHGGRVMA
                     QGTAADVAANPESLTGRYLAGALRIAVPARRMPRRGQAWGAHGDTATELRIVGARGNN
                     LKNVSVEIPVGLLTCVTGVSGSGKSTLVNDTLYASVARKLYNSHAEPEPVDSIEGLDA
                     FDKVINVDQSPIGRTPRSNPATYTGMFTPIRELFAEMNTAKERGYGPGRFSFNVAGGR
                     CEACQGDGVLKVEMHFLPDVYVPCDVCHGKRYNRETLEVLYKGKNITEVLDLTVEDAH
                     AYFSAVPNIARRLQTLLDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRDTGKTLYI
                     LDEPTTGLHFADIDLLLKVLHQLRDAGNTIVVIEHNLDVIKTADWIIDMGPEGGAGGG
                     TVLVAGTPEDVAASPASFTGRYLRPLLGA"
     gene            complement(3370..4362)
                     /locus_tag="VFA35_10390"
     CDS             complement(3370..4362)
                     /locus_tag="VFA35_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56625.1"
                     /translation="MNTPRHDDPERDAWLSEALRHAPDAQAAPPAALRDAILRQARAA
                     AHAARVPAPASARGPWAVAWDWLARPRVAAGFASVMVATLVGVMWWGQPIDRTLAPPP
                     AVDATPAPAATPPAAPAAPPEFAPDAPRQAATPAPRRQPARAAPPAAPSPAPAPTPTP
                     AQVPALRERAPPPLTAAAPSPVEAARDEARAPNAAAVSRGIVAKAAAPAAERATDAVD
                     ARPATLRALLAAVAAQPERWGWERSGAVQALTPALQRWLAQLDAASAGVATRAAAETA
                     PSGEASVLRLYRDGALAATLRLDDTSAGLVPGPRVALPPATLAALKQALDDATR"
     gene            complement(4448..>5053)
                     /locus_tag="VFA35_10395"
     CDS             complement(4448..>5053)
                     /locus_tag="VFA35_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZT56626.1"
                     /translation="MTAYAAGDARAFEQLYARHQAGLYRFIRRLLGSALNAQTDEVFQ
                     DTWLRVVHARARWEPQGASFRTWLFTLAHHRVVDLLRRSGRELSVDAFEGADGAPWEP
                     RAEASAAWRHWPAPAAAARTEELAFWRRAGEKLLGCLEQLPVPQRSAFLLHHDDGLAL
                     DEVASALEVGFETAKTRLRYAMSKLRTCMGAYLAPLGHEAR"
BASE COUNT          618 a         1775 c         1901 g          759 t
ORIGIN      
        1 gggcctgcgt ggtggagcga tgcagcgccc atggccgggt tcggcctgcc gctggtggtg
       61 ccgggcatcg tgggcggcgt cggtggtggc ggtgagtcga ccgcgatgac cctggagccg
      121 ccgcgcggcg ccgatggcgg cctgccgccg atcgcgccgc cgccgatcac ggccccggtg
      181 cccgaaccgc agacctgggc gctgatgctg gccggcctcg ccgcgggggc gctggtgcgg
      241 cggcgggtcg ccaggcgctg aacaacgatc gcggggcgtc aggcgcccag cagcggccgc
      301 agataacgcc cggtgaagct cgccgggctc gcggccacgt cttccggcgt gccggcgacg
      361 agcaccgtgc cgccgccggc cccgccctcc ggccccatgt cgatgatcca gtcggcggtc
      421 ttgatcacgt cgaggttgtg ctcgatcacg acgatggtgt tgccggcgtc gcgcagctgg
      481 tgcagcacct tgagcagcag gtcgatgtcg gcgaagtgca gcccggtggt cggctcgtcc
      541 aggatgtaga gcgtcttgcc ggtgtcgcgc ttgctgagtt cgagcgcgag cttgacgcgc
      601 tgcgcctcgc cgccgctcag tgtcgtcgcg ctctggccga ggcggatgta gcccaggccc
      661 acgtcgagca gcgtctgcag ccggcgcgcg atgttgggca ccgcgctgaa gtacgcatgc
      721 gcgtcttcga cggtgaggtc gagcacctcg gtgatgttct tgcccttgta gagcacctcg
      781 agcgtctcgc ggttgtagcg cttgccgtgg cacacgtcgc agggcacgta gacgtcgggc
      841 aggaagtgca tctcgacctt cagcacgccg tcgccctggc aggcctcgca gcgcccgccg
      901 gcgacgttga agctgaagcg cccggggccg tagccgcgct ccttcgcggt gttcatctcg
      961 gcgaacagct cgcggatcgg cgtgaacatg ccggtgtagg tggccgggtt gctgcgcggc
     1021 gtgcggccga tcgggctctg gtcgacgttg atgaccttgt cgaacgcgtc gagcccctcg
     1081 atcgaatcga ccggctcggg ctccgcgtgg ctgttgtaga gcttgcgcgc gacgctcgcg
     1141 taaagcgtgt cgttgaccag cgtgctcttg cccgagcccg agacgccggt cacgcaggtc
     1201 agcaggccca ccgggatctc gacgctgacg ttcttcaggt tgttgccgcg cgcaccgacg
     1261 atgcgcagct cggtcgcggt gtcgccatgc gcaccccagg cctggccgcg ccgcggcatg
     1321 cgccgcgccg gcaccgcgat ccgcagcgcg cccgccaggt accggcccgt gagcgactcg
     1381 gggttcgccg cgacatccgc cgcggtgccc tgcgccatca cgcgcccgcc gtgcacgccg
     1441 gcgcccgggc ccatgtcgat cacgtgatcg gcggcgcgga tcgcgtcctc gtcgtgctcg
     1501 accaccagca cgctgttgcc gatgtcgcgc agatggcgca gcgtgccgat caggcgctcg
     1561 ttgtcgcgct ggtgcaggcc gatgctgggc tcgtcgagca cgtacatcac gccggtcagc
     1621 cccgagccga tctgcgacgc gaggcggatg cgctgcgact cgccgcccga gagcgaatcg
     1681 gcattgcggt ccaggctcag gtagttcagc cccacgtcgt tcaggaactt cagccgcgag
     1741 cggatctctc gcaccacctt gtcggcgatc tcggccttcg cgccctgcaa ggtgagcgac
     1801 tcgaagtacg cgaggcactc gcgcagcgtg aagtgctcga cgcgataaat gggctcgcgc
     1861 cgtgggcgct cggtgggctc gcaccgcggg cgctcggtcg gctcctttgc ggcgctggtc
     1921 gcgtcgacca ggaacacgtg gcgtgcctcg cgccgtagcc gcgtgccgtt gcagtcgggg
     1981 cagggcttcg cgctttggta gcgcatcagg tcctcgcgca ccgcggccga atcggtctcg
     2041 cggaagcgcc gctccaggtt cggcaggatg ccctcgaacg ggtgcgagcg cttgaccatg
     2101 cgctgtttgc cgcgcggccc atcggcctcg tagacgaact cgatgtcggt cgcgcccgag
     2161 ccgtgcagca gcaccttttg cgcgctcttc ggcagttcct cgaacgcggc gtcgatgtcg
     2221 aatgcgtagt gcttcgcgac gctttcgagc agcgagaagg tgtagccgtt gcgccggtcc
     2281 cagcccttga ccgcgccgct ggccaggctc agcgacggga aggcgacgac gcgctcgggg
     2341 tcgaacacgg tcgtgtggcc gaggccgtcg caggtcgggc aggcgccgac cggcgagttg
     2401 aacgagaaca ggcgcggctc gagctcgctc aggctgtagc tgcagaccgg gcaggcgaac
     2461 ttgctcgaga acaggtgcgc cttgtcggtg tccatctcga gcgcgatcgc gcggccgtcg
     2521 gcgatgcgca gcgccgcctc gaagctttcg gcgagccgtt gctgcaagcc ggcaaccacc
     2581 ttgacgcggt cgatcacgac gtcgatgtcg tgcttctcgg ccttcttcaa cttcggcaag
     2641 tccgcggctt cgcaggtctg gccgtcgacg cgaaagcgga tgtagccctg cgcctgcatc
     2701 tcggcgaaca gctcggtgaa ctcgcccttg cggtcgcgca cgaccggcgc gacgatcatc
     2761 agccgcgtcc cttcgggcag cgcgagcacg gcgtcgacca tctggctcac cgactgcgcc
     2821 tgcagcggca ggtcgtggtc tgggcagtag ggcgtgccgg cgcgcgcata cagcaggcgc
     2881 aggtagtcgt ggatctcggt gacggtgccg acggtggagc gcgggttgtg cgaggtcgcc
     2941 ttctgctcga tgctgatcgc gggcgagagg ccctcgatca cgtcgacatc gggcttgtcc
     3001 atcagctgca ggaactggcg cgcgtacgcc gacaggcttt ccacgtaacg ccgctggccc
     3061 tcggcataca gcgtgtcgaa cgcgagcgag ctcttgcccg aacccgacag gccggtgatc
     3121 accacgagct ggttgcgcgg gatgtcgagg tcgatgttct tcaggttgtg ggtgcgcgcc
     3181 ccgcgaacgc ggatcgcgtt cgcggcctcc gcgggctgaa cgcggatcgt cgtcgcttcg
     3241 ggcggcgacg cgcgtgcggc ggaagagtcg gcggggtcgg gcatcgggcg ttcgcgcaaa
     3301 accgaccatg ataagggtca tgcggcgcac gggccgctcg ccccgctgcg ccggactcat
     3361 cgcgcccgct cagcgggtgg cgtcgtcgag cgcctgcttc agcgcggcga gcgtggccgg
     3421 cggcagggcg acgcgcgggc ccggtaccaa gccggcgctc gtgtcgtcga gccgcagcgt
     3481 cgcggccagc gcgccgtcgc ggtacaggcg cagcacgctg gcctcgcccg acggcgcggt
     3541 ctcggccgcg gcgcgggtgg cgacgccggc gctggcggca tcgagctgcg cgagccagcg
     3601 ctgcaatgcc ggtgtcagcg cttgcaccgc gccgctgcgc tcccagcccc aacgttcggg
     3661 ttgcgcggcg acggcggcga gcagcgcgcg gagcgtggcc ggtcgcgcgt ccacagcatc
     3721 cgtggcgcgc tcggccgcgg gggcggcggc cttggcgacg atgccgcgcg acacggcggc
     3781 ggcgttcggc gcgcgcgcct cgtcgcgtgc cgcctcgacc gggctcggcg cggccgcggt
     3841 caacggcggc ggcgcccgtt cgcgcagcgc cggcacctgg gcgggcgtgg gcgtgggcgc
     3901 gggtgcgggc gaaggcgcag ccggcggcgc ggctcgcgct ggctgtcgcc gcggcgccgg
     3961 cgtggcggcc tggcgcggcg cgtcgggcgc gaactcgggc ggcgcggcgg gcgccgcggg
     4021 cggtgttgcc gccggcgccg gggtcgcatc gaccgccggt ggcggtgcga gggtccgatc
     4081 gattggctgg ccccaccaca tcacgccgac cagcgtcgcg accatcacgc tcgcgaaccc
     4141 ggccgcgacc cgcgggcgcg cgagccaatc ccatgcgacg gcccacgggc cgcgcgccga
     4201 tgccggcgcg ggcacccgcg ccgcatgcgc tgccgcgcgc gcctggcgca ggatcgcgtc
     4261 gcgcagcgcc gcgggcggcg cggcctgcgc gtcgggcgcg tggcgcagcg cttcgctgag
     4321 ccaagcgtcg cgttccgggt catcgtgccg cggcgtgttc atgacgactc cccgatcgcc
     4381 gggtacggcg atcggccctc cacgagggtc gggcctcgct tgggacggcc cggcgcggcg
     4441 gcccgcatca tcgcgcttcg tgccccagcg gcgccaggta ggcgcccatg caggtgcgca
     4501 gtttgctcat cgcatagcgc agccgcgtct tcgcggtctc gaagccgact tcgagcgcgc
     4561 tcgcgacctc gtcgagcgcc aggccgtcgt cgtggtgcag caggaacgcg ctgcgctgcg
     4621 ggaccggcag ctgttcgagg cagcccagca gcttctcgcc ggcgcggcgc cagaacgcga
     4681 gctcttcggt gcgtgcggcg gcggccggcg cgggccagtg ccgccacgcg gccgaggcct
     4741 cggcgcgcgg ctcccacggc gcgccgtctg cgccctcgaa tgcgtcgacc gagagctcgc
     4801 ggccgctgcg ccgcagcagg tcgacgacgc ggtggtgcgc gagcgtgaac agccaggtgc
     4861 gaaagctcgc gccctgcggc tcccagcgcg cgcgcgcgtg cacgacgcgc agccaggtgt
     4921 cctggaacac ctcgtcggtc tgcgcattca gcgcgctgcc gagcaggcgg cggatgaagc
     4981 ggtacaggcc ggcctggtgg cgcgcataca gttgctcgaa cgcgcgcgcg tcgccggccg
     5041 cgtaggcggt cat
//
LOCUS       DASRDG010000620         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_899594, whole genome shotgun sequence.
ACCESSION   DASRDG010000620 DASRDG010000000
VERSION     DASRDG010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899594"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2088)
                     /locus_tag="VFA35_10400"
     CDS             complement(<1..>2088)
                     /locus_tag="VFA35_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZT56627.1"
                     /translation="ARWQSDGRRCTCIFHTPAPPATDRDSALARRSPRAQAALFRLLA
                     DNVPVLIAYYSAHDFRCQFANKRYAQTFGRDEHAIVGSTLEEVIGADAAREIQPQTER
                     VLALREPVVYERALGGSARRWLEVHLLPHLDEATGAAIGLFVLISDITKHRHAEQAAR
                     ESEERLAKFMQANAEGIVFHRDGFITDANPPLCALIGYSLSELLGRRTLDFIAPDHVA
                     KVAAVFASGQETGYESVLLDKHGRRIPVEFIVRTMTHGGEHLRMTIVRDIRDRHAAQA
                     RIHHLAHHDVLTGLPNRLSFLAHLAQQMAPGPDGQPARLALLFIDLDHFKRVNDSLGH
                     LVGDTLLRTVAARITAALRATDLVSRFGGDEFVVLLSGLAPGVQQRADAEEVAQKLLT
                     GIGAPLDAEGRPLSVTPSIGIALYPGDGGSPEELVKHADSAMYLAKARGRANHQFFDR
                     GRANSAYAELVMEGQLAQALERGEFVLHFQPQVRARDGRLVGAEALIRWNHPERGLLL
                     ADAFIPLAEQRRLMLPIGQWVLAEAARCAVRWHQMGLGVAPVAVNLSSVQFHSIGFLE
                     GVAQVLPSDGIGAGLLELELTERMLIDDLVEVKQRLAHLKAMGLQISVDDFGTGYSSL
                     RHLKELPIDKVKIDRSFIHDLPDNGDSAAITRAIIQMGRNLGMTVIAEGVETEAQRAF
                     LAAHGCDELQGLLI"
BASE COUNT          307 a          731 c          738 g          312 t
ORIGIN      
        1 gatcaacaac ccctgcaact cgtcgcaccc gtgtgcggca aggaacgcgc gttgggcttc
       61 tgtctcgacg ccttcggcga tcacggtcat gccgaggttg cgccccatct ggatgatcgc
      121 gcgcgtgatc gcggccgaat cgccgttgtc cggcaggtcg tggatgaacg aacggtcgat
      181 cttgaccttg tcgatcggca gctccttcag gtgccgcagc gacgagtagc cggtgccgaa
      241 gtcgtcgacg gagatctgca gccccatcgc cttcaggtgc gcgaggcgct gcttcacctc
      301 gaccaggtcg tcgatcagca tgcgctcggt cagctcgagt tcgagcagcc ccgcgccgat
      361 accgtcgctg ggcaacacct gcgcgacccc ttcgagaaag ccgatcgaat ggaactgcac
      421 gctcgacagg ttgaccgcga ccggcgcgac gcccaagccc atctggtgcc agcgcaccgc
      481 gcagcgcgca gcctcggcca gcacccattg cccgatcggc agcatcagcc ggcgctgctc
      541 ggccagcggg atgaacgcat cggcgagcag caggccgcgc tcggggtggt tccagcggat
      601 cagcgcctcg gcgccgacca agcggccgtc gcgggcccgc acctgcggct ggaagtgcag
      661 cacgaactcg ccgcgctcca gcgcctgcgc gagctggccc tccatcacga gctcggcata
      721 ggccgagttc gcccgcccgc ggtcgaagaa ctggtggttc gcgcggccac gcgccttcgc
      781 gaggtacatc gccgagtcgg cgtgcttgac cagttcctcg ggcgagccgc cgtcgccggg
      841 gtacagcgcg atgccgatcg acggcgtcac cgacagcggc ctgccttcgg catcgagcgg
      901 cgcgccgatg ccggtgagca gcttttgcgc gacctcctcg gcatcggcgc gctgctgcac
      961 gccgggcgcc aggcccgaca gcagcacgac gaactcgtcg ccaccgaagc gcgagaccag
     1021 gtcggtcgcc cgcagcgccg cggtgatgcg cgcggccacg gtgcgcagca gggtgtcgcc
     1081 gaccaggtgg ccgagcgagt cgttgacgcg cttgaagtgg tcgaggtcga tgaacagcag
     1141 cgccagccgc gccggctggc cgtccggccc cggcgccatc tgctgcgcca ggtgcgccaa
     1201 gaacgacagc cggttcggca ggccggtcag cacgtcgtgg tgcgcgaggt ggtggatgcg
     1261 cgcctgcgcg gcgtggcggt cgcggatgtc gcgcacgatc gtcatgcgca agtgctcgcc
     1321 gccgtgcgtc atcgtgcgca cgatgaattc gaccgggatg cgccggccat gcttgtcgag
     1381 cagcacgctc tcgtagccgg tctcctggcc cgaggcgaac accgcggcga ccttggcgac
     1441 atggtcgggc gcgatgaagt cgagcgtgcg ccggccgagc agttcgctca gcgagtagcc
     1501 gatcagcgcg cacaacggcg ggttcgcgtc ggtgatgaag ccgtcgcggt ggaacacgat
     1561 gccttcggcg ttggcctgca tgaacttcgc gagccgctct tccgattcgc gcgcggcctg
     1621 ctcggcgtgc cggtgcttgg tgatgtcgct gatcagcacg aacaggccga tcgccgcacc
     1681 ggtcgcttca tccaggtgcg gcagcaggtg cacctcgagc cagcgccggg cgctgccgcc
     1741 cagcgcgcgc tcgtagacca ccggctcgcg cagcgcgagc acgcgctcgg tctgcggctg
     1801 gatttcgcgc gccgcgtcgg cgccgatcac ctcctcgagc gtcgagccga cgatcgcgtg
     1861 ctcgtcgcgg ccgaaggtct gcgcgtaccg cttgttcgcg aactggcagc ggaaatcgtg
     1921 cgcgctgtag tacgcgatca gcaccggcac gttgtcggcc agcagccgga acagcgccgc
     1981 ctgggcccgc ggcgaacggc gcgcgagtgc gctgtcgcga tccgtggcgg gcggcgccgg
     2041 tgtgtggaag atgcacgtgc agcgccggcc gtcggactgc cagcgcgc
//
LOCUS       DASRDG010000621         5227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_327562, whole genome shotgun sequence.
ACCESSION   DASRDG010000621 DASRDG010000000
VERSION     DASRDG010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327562"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VFA35_10405"
     CDS             complement(<1..1452)
                     /locus_tag="VFA35_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZT56628.1"
                     /translation="MNDILGIAGPVTGRRHAFRRALRLPLGLLLALQLIIAPARAHAE
                     PDQALPELRAAEFSADGGVTWSHVALPDTWSLRGLPPRGSASYRLQLTLAHVPTQMWA
                     LRADRISSAYTLYVNDRLADAGSGAAAHNGRPIAHLIDLPPDLLHAGTNDIRIELDYR
                     NRGGLSALALGPLPLLQGPFARHVALTEILPECLNIAGAAFGLFMCAIWWRRRGEAAI
                     GWFGVLCMLVSVRNYCYFVVDGPALPDALSNWAYYVAQVASALLFGAFALALSGERLR
                     WFERTLQGSAVVLLAGSVLAGGTDLQTLRALTYPLVIALSLVSLWLVVAHARRVRQHA
                     LLVLPCGLAMVLLGGVHDYLYQQGHLSVLGGYWMPYATPVALFSFALVLVNRLVQALA
                     DVELLNASLERRVADRTHALEVANASKTRFLAAASHDMRQPVVTIGLLVGLVREQLAA
                     LPAVRPMLDRIHEAVASLEALLKGLMDLSRLESG"
     gene            complement(1522..3297)
                     /locus_tag="VFA35_10410"
     CDS             complement(1522..3297)
                     /locus_tag="VFA35_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56629.1"
                     /translation="MKHSLWIPTVLSTAILAACGGGGDDAGSASSNGTGPTTPAALTL
                     TGVAATGAAIGGKTVEAKCSTGTGSAMSNPDGSYTISVTSGVLPCVLKVTPASGPALY
                     SLATGSGSAATANISPVTQLVMASLTGTDPDAYFTGFDATAAAAVTSSKVSDAVAAVK
                     TTLLAAGVDLGAIDVLAGTLTPATTTTTGNAYDQALDALAAKLAAAGTTLTALTTSVV
                     ATSPAAPTTVTTAAGSTASLPAELLLRPAAPSCASLRSGTYRVVAPTPPTPSMPSTLA
                     IEVGTITINAVTGAVVYSDGSPGTWTAHPTEHCRFTDETGQSDVVVSPAGVIVGRSYQ
                     TLSNTDNTFRPVIAFPEQSHTLADVAGAWNVIGVNPNDANTAYYGVAGTGTLDANGAI
                     TAISVCGNDGSWNLATTCVTPTDAQSTRRLNPAGGFDVVEPGGYVSGRGFVYRAGGGA
                     LMRVEVYDRGDFTVWTQQRKNDLPAIGRVNTSWDLRISNTMLATPVLSESTNTVQTVD
                     ATASSFVRLAKTVGGTDEHLETVLVNNPRDGYNFRAAATVLGTDGTTMVPVSEWTNLN
                     LRGMGLNALTRPAQKQFMFSVQQPS"
     gene            complement(3533..4582)
                     /gene="queA"
                     /locus_tag="VFA35_10415"
     CDS             complement(3533..4582)
                     /gene="queA"
                     /locus_tag="VFA35_10415"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZT56630.1"
                     /translation="MPPDHRLSDFDFELPIELIAQTPAAERSASRLLDGTGTAPVDRV
                     FRDLPTLLKAGDLLVFNDTRVIKARLHGSKASGGAVEALVERVLANHEVLMHLRASKS
                     PKAGSVVRFADAFDAEVLGRAGPQDALFHLRFPSDPFALLEAHGHVPLPPYITHADTA
                     EDERRYQTVFAANPGAVAAPTAALHFDAALLAELAQRGIARASVTLHVGAGTFQPVRT
                     ENLAEHTMHSEWFDVPQATVDAIARTRAAGGRIVAVGTTTLRALESAARGGELEPGSR
                     ETDIFITPGFTFRVVDLLVTNFHLPKSTLVMLVSAFAGFDRVRALYAHAIGARYRFFS
                     YGDAMLLERKAGPTA"
     gene            complement(4643..>5227)
                     /locus_tag="VFA35_10420"
     CDS             complement(4643..>5227)
                     /locus_tag="VFA35_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56631.1"
                     /translation="MISTLFRRTAGSVLVAVAALFTGCAQVPLGTPVASIDNIQAARA
                     VGMQPVNVGKFELAAGKDPTLDKGLAIRAASVGSPIDGSFAKYLRETLTVELKAAGLF
                     DPTAKTTIEGFLTDSAADAAMSRGTGRLGARFVVVRGGRTVYDKELKVDASWDSSFVG
                     AVAIPAAINEYSALYRKLVAKLLADPAFRAAVTA"
BASE COUNT          763 a         1740 c         1960 g          764 t
ORIGIN      
        1 gcccgactcg agccgcgaca aatccatcag gcctttcagc agcgcctcga gcgaggccac
       61 cgcctcgtgg atccggtcga gcatcgggcg caccgcgggc agcgccgcga gctgctcgcg
      121 caccaggccg acgagcagcc cgatcgtcac caccggctgg cgcatgtcgt ggctggcggc
      181 ggcgaggaag cgcgtcttcg aggcgttcgc gacctcgagc gcgtgggtcc ggtcggcgac
      241 ccgccgctcc agcgacgcgt tgagcagctc gacgtcggcg agcgcctgca ccagccggtt
      301 caccagcacc agggcgaagc tgaacagcgc caccggcgtg gcgtagggca tccaatagcc
      361 gccgagcacc gagaggtggc cctgctggta gaggtagtcg tggacgccgc cgagcagcac
      421 catcgccagg ccgcacggca gcaccagcag cgcgtgctgc cgcacccggc gcgcatgcgc
      481 gaccaccagc cacagcgaga ccagcgacag cgcgatcacc agcgggtagg tcagcgcgcg
      541 cagcgtctgc aggtcggtcc cgccggccag caccgagccc gccagcagca ccacggccga
      601 gccctgcagc gtgcgctcga accagcgcag gcgctcgccc gacaacgcga gcgcgaacgc
      661 gccgaacagc agcgcgctgg cgacctgggc gacgtagtag gcccagttgc tcagcgcatc
      721 gggcagggcc gggccgtcga ccacgaaata gcagtagttg cgcaccgaca ccagcatgca
      781 cagcacgccg aaccagccga tggccgcctc gccgcggcgg cgccaccaga tcgcgcacat
      841 gaacaggccg aaggccgcgc cggcgatgtt caggcactcc ggcaggatct cggtcagcgc
      901 cacgtggcgc gcgaacgggc cctgcagcag cggcagcggg cccagcgcca gcgcggacag
      961 gccgccgcgg ttgcggtagt cgagctcgat gcggatgtcg ttcgtcccgg catgcagcag
     1021 gtcgggcgga agatcgatca ggtgcgcgat cgggcggccg ttgtgcgccg ccgcgccgct
     1081 gcccgcatcg gccaggcggt cgttgacgta aagcgtgtag gcgctgctga tgcggtcggc
     1141 gcgcagtgcc cacatctgcg tcggcacgtg cgccagcgtc agctgcagcc ggtagctggc
     1201 gctgccgcgc ggcggcagcc cgcgcagcga ccaggtgtcg ggtaatgcga catgcgacca
     1261 cgtgacgccg ccgtcggccg agaattcggc cgcacgcagt tcgggcagcg cctgatcggg
     1321 ttctgcgtgg gcgcgggccg gtgcgatgat cagctgcagc gccagcagca gccccagcgg
     1381 caagcgcaag gcccgccgaa atgcgtgtcg ccggcccgtg accgggccgg cgattcccaa
     1441 gatgtcgttc atcgtgaccc tgcgatggcg gccggttggc cccggtcttt caaatccgag
     1501 cttgcaaccg gccccgctgc gtcaggaggg ttgctgcacc gagaacatga actgcttctg
     1561 cgccgggcgc gtcagcgcat tcagacccat gccgcgcagg ttgaggttgg tccactcgga
     1621 cacggggacc attgtcgtgc cgtcggtgcc gagcacggtc gcggcggcgc ggaagttgta
     1681 gccgtcacgc gggttgttga cgagcacggt ctcgaggtgc tcgtcggtgc cgcccacggt
     1741 cttggcgagc cgcacgaagc tgcttgcggt cgcgtcgacg gtctggacgg tgttggtcga
     1801 ctcgctcagg accggcgtgg ccagcatcgt gttgctgatc ctcaggtccc agctcgtgtt
     1861 gacgcggccg atcgccggaa ggtcattctt gcgctgctgc gtccagaccg tgaagtcgcc
     1921 gcggtcgtag acctcgacac gcatcagcgc gccgccgccg gcgcgataga cgaagccgcg
     1981 cccggagacg tagccccccg gctcgacgac gtcgaagccg ccggccgggt tcagccgacg
     2041 cgtcgactgc gcgtcggtgg gggtgacgca ggtcgtcgcc aggttccagc tgccgtcgtt
     2101 gccgcacacc gagatcgcgg tgatggcgcc gttggcgtcc agcgtgccgg tgcccgcgac
     2161 gccgtagtaa gcggtgttgg cgtcgttggg gttcacgccg atcacgttcc aggcgccggc
     2221 cacgtccgcc agcgtgtggc tctgttcggg gaaggcgatg acgggacgga aggtgttgtc
     2281 ggtgttgctg agcgtctggt acgagcggcc gacgatcacg ccggccggcg acacgacgac
     2341 gtcggattgg ccggtctcgt cggtgaagcg gcagtgctcg gtggggtggg ccgtccaggt
     2401 gccaggagag ccgtcgctgt agacgactgc gccggtgacg gcgttgatcg tgatcgtgcc
     2461 gacctcgatg gccagggtcg acggcatcga cggtgtcggc ggcgtcggcg ccaccacccg
     2521 gtaggtgccg ctgcgcagcg acgcgcagct cggggcggcc ggcctgagta gcagctcggc
     2581 gggcagcgaa gccgtgctgc cggcggccgt ggtcaccgtc gtgggcgccg ccggcgacgt
     2641 ggcgacgacc gaggtggtca gcgcggtcag cgtcgtgccg gcggcggcga gcttggcagc
     2701 gagcgcgtcg agcgcctggt cgtaggcatt gccggtcgtc gtcgtggtgg cgggtgtcag
     2761 cgtgccggcc agcacgtcga tggcgccgag gtcgacgccg gcggccagca gcgtggtttt
     2821 cacggcggcg acggcgtcgc tgaccttgct gctcgtcacc gccgctgccg cggtcgcgtc
     2881 gaagccggtg aagtaggcgt cggggtcggt gccggtcagg ctcgccatca ccagctgcgt
     2941 gacggggctg atgttcgcgg tggcggcgct gccgctgccg gtggccagcg agtacagcgc
     3001 cgggccgctg gccggcgtga ccttgagcac gcagggcagc acgcccgacg tgacgctgat
     3061 cgtgtagctg ccgtcggggt tcgacatcgc gctgccggtg ccggtgctgc atttcgcctc
     3121 gacggtcttg ccgccgatgg ccgcgccggt ggccgcgacg cccgtcaacg tgagcgccgc
     3181 gggcgtggtc gggcccgtgc cgttgctaga cgcgctgccc gcgtcgtcac caccgccgcc
     3241 acaggcggcc aggatggcgg tgctgagcac ggtcgggatc cagagggaat gcttcacggt
     3301 gatgttcctt tgtccaacga tgggaatgag gggcgcccgc aacgggccgg cacacgccgc
     3361 cgagttcgag gtcggcgctg ttggtgctga agtcattctc gaaacgccgc cgcagctggg
     3421 gtatcggcca aatggtcgag tggccgcccg gggagtcatg gcgtgtgaac aatgtcgcgg
     3481 cgtgcgagcc gcccgaggca gcggcgtcgc aacgcgtggc gcggaacaaa cgtcaggcgg
     3541 ttgggcctgc ttttcgttcg agcagcatcg cgtcgccgta gctgaagaag cggtagcgcg
     3601 cgccgatcgc atgcgcgtac agcgcgcgca cgcggtcgaa gccggcgaac gcgctgacca
     3661 gcatcaccag cgtgctcttg ggcagatgga agttggtgac cagcaggtcg acgacacgga
     3721 acgtgaagcc cggcgtgatg aagatgtcgg tctcgcggct gccgggttcg agctcgccac
     3781 cgcgtgctgc ggattcgagc gcgcgcagcg tcgtcgtgcc gacggcgacg atgcgcccgc
     3841 ccgccgcgcg ggtgcgtgcg atcgcatcga cggtggcttg cggcacgtcg aaccactcgc
     3901 tgtgcatcgt gtgctcggcg aggttctcgg tgcgcaccgg ctggaaggtg ccggcgccga
     3961 cgtgcagcgt gacgctcgcg cgtgcgatgc cgcgttgcgc gagttcggcg agcagcgccg
     4021 cgtcgaagtg cagcgcggcg gtcggcgcgg cgaccgcgcc ggggttcgcg gcgaacacgg
     4081 tctggtagcg gcgttcatct tccgcggtgt cggcgtgcgt gatgtacggc ggcagcggca
     4141 cgtggccgtg ggcttcgagc agcgcgaacg ggtcggacgg gaaacgcaga tggaacagcg
     4201 catcctgcgg cccggcgcgg ccgagcacct cggcgtcgaa cgcatcggca aagcgcacca
     4261 cgctgcccgc cttcggcgac ttgctggcgc gcaggtgcat cagcacctcg tggttcgcca
     4321 gcacgcgctc gacgagcgcc tcgaccgcgc cgccgctggc tttgctgccg tgcagccgtg
     4381 ccttgatcac gcgcgtgtcg ttgaagacca gcaggtcgcc ggccttcagc agcgtgggca
     4441 gatcgcggaa cacgcggtcg accggcgcgg tgccggtgcc gtcgagcagg cgcgaggcgc
     4501 tgcgctcggc ggcaggggtc tgcgcgatca gttcgatagg aagctcgaag tcgaagtcgc
     4561 tcagacgatg gtcgggaggc atggggcggc ggaggggcgc agtgcgcggg cccgcattat
     4621 cggaccgcgc ctaccctcgg cgctatgcgg tgaccgcggc gcggaacgcc ggatccgcaa
     4681 gcagcttggc gaccagcttg cggtagagcg ccgagtactc gttgatcgcc gcgggaatgg
     4741 cgaccgcgcc gacgaaggac gaatcccagc ttgcatcgac cttcagttcc ttgtcgtaga
     4801 ccgtgcggcc gccacgaacg acgacgaagc gcgcgccgag gcggccggtg ccccgactca
     4861 tcgccgcgtc ggcggcgctg tcggtcagaa agccctcgat cgtcgtcttc gctgtcggat
     4921 cgaacagccc ggcagccttc agctcgacgg tcagcgtttc gcgcaggtac ttcgcgaagg
     4981 agccgtcgat cggcgagccg acggaggcgg cgcggatcgc gaggcccttg tcgagggtcg
     5041 ggtccttgcc ggccgcgagc tcgaacttgc cgacattcac cggctgcatg ccgacggccc
     5101 tggcggcctg gatgttgtcg atgctggcca cgggcgtgcc aagcggcacc tgcgcgcacc
     5161 cggtgaacag ggctgcaacc gcgaccagca cgctgccggc ggtgcggcga aaaagcgtgg
     5221 aaatcat
//
LOCUS       DASRDG010000622         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_210278, whole genome shotgun sequence.
ACCESSION   DASRDG010000622 DASRDG010000000
VERSION     DASRDG010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210278"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VFA35_10425"
     CDS             complement(<1..1285)
                     /locus_tag="VFA35_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(698..700),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZT56632.1"
                     /translation="MKLSRRQFMKVTGSTLAGSSLALMGFSPATALAEVRQYKLAATT
                     VTRQTCTYCSVGCGILMYSLGDNAKNAKRSVIHVEGDPDHPVNRGTLCPKGASLLDIV
                     NSPSRLLYPEYRAPGSNEWKRMSWDDALDRIAKLLKADRDANFVETTAEGRRVNRWTS
                     SGMLAASAASNEAGYITHKIARSWGMLAFDNQARVUHGPTVAGLAPTFGRGAMTNHWV
                     DIKNADVILVMGGNAAEAHPCGFKWVTEAKAHNKAHFMVVDPRFNRSASVADFYAPIR
                     SGSDIVFLGAMINYLITNDKIHHEYVKAYTDFTFIVREDFQFDEGIFSGYHEDKRQYD
                     KTSWDYELGADGFVKTDPTLKHPRCVFQLLKAHYASYTPEKVESACGTPKAKFLHVCE
                     QFASTAVPGRAATILYALGWTQHDIGAQTLRCAAMV"
     gene            1658..3037
                     /gene="selA"
                     /locus_tag="VFA35_10430"
     CDS             1658..3037
                     /gene="selA"
                     /locus_tag="VFA35_10430"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZT56633.1"
                     /translation="MNPLPLHPPSVDRLLDTPALRALIEQHGRTGVVQAVREQLDAWR
                     AAAASGFDGARFEQACRERLDARAASSLRPVFNLTGTVLHTNLGRAVLPPEAVDAVAA
                     VMRQPSNLEYELGGGARGDRDDHVEGLLRELTGAQAATAVNNNAAAVLLMLNTLALGK
                     EVPVSRGELIEIGGAFRIPDIMRRAGAKLVEVGTTNRTHLRDYAEAIGPDTAALMKVH
                     TSNYVIEGFTAAVAEAELAALARERGLPLIVDLGSGSLVDLTRWGLSPEPTPRQALAS
                     GANLVSFSGDKLLGGPQAGLIVGDADLVARIKRNPLKRALRLDKMTLAALEAVLKLYR
                     DPDRLAQRLPTLRALTRPVAELQALAERLAPALRAALGPLAQVQVRACRSQIGSGSLP
                     VDRLASICLGVGAAAGGSGGYPDKLAAAFRALPVPVIGRIEEGELRLDLRCLDDEASF
                     VAQLTALVA"
     gene            complement(3051..3593)
                     /locus_tag="VFA35_10435"
     CDS             complement(3051..3593)
                     /locus_tag="VFA35_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZT56634.1"
                     /translation="MDHLGDVARDALNRNSFVGAALLGALIFGLASVVVVLIRRTARR
                     VGQHLSDVTALRFVSSLAQLLTYLIAFALCAHLVPELRVFGTALLAGASVFSVVVGLA
                     AKDALGCLIAGFLLVLYRQVRVGDTIRVDSPAGVVTATVQVISLGFTILVDDQKAEVV
                     VPNNVMMGSTIIRVARGPER"
BASE COUNT          584 a         1292 c         1284 g          597 t
ORIGIN      
        1 gcaccatcgc cgcgcagcgc agggtctgcg cgccgatgtc gtgctgcgtc cagcccaggg
       61 cgtacaggat ggtcgcggcg cgcccgggca cggccgtcga tgcgaactgc tcgcacacgt
      121 gcaggaactt cgccttcggc gtgccgcacg cgctctccac cttctcgggc gtgtagctcg
      181 cgtagtgggc cttcagcaac tggaagacgc agcgcgggtg cttcagcgtc ggatcggtct
      241 tgacgaagcc gtcggcgccc agttcgtagt cccagctggt cttgtcgtac tggcgcttgt
      301 cctcgtggta gcccgagaag atgccttcat cgaactggaa gtcctcgcgc acgatgaagg
      361 tgaagtcggt gtacgccttc acatactcgt ggtggatctt gtcgttcgtg atcaggtagt
      421 tgatcatcgc cccgaggaag acgatgtctg acccagagcg gatcggcgca tagaagtcgg
      481 ccaccgacgc cgagcggttg aagcgcgggt cgaccaccat gaagtgcgcc ttgttgtgcg
      541 ccttcgcttc cgtcacccac ttgaagccgc aggggtgcgc ctcggcggca ttgccgccca
      601 tcaccagaat cacatccgcg ttcttgatgt cgacccaatg gttcgtcatc gctccacggc
      661 caaacgtcgg ggcaagacct gccaccgtcg gaccgtgtca aacgcgtgcc tggttgtcga
      721 atgcgagcat gccccaggag cgggcaatct tgtgcgtgat gtagccggcc tcgttgctgg
      781 cggccgaggc cgcgagcatg ccggacgagg tccagcggtt gactcgtctg ccctccgccg
      841 tcgtctcgac gaagttggcg tcgcggtcgg ccttcagcag cttggcgatg cggtccagcg
      901 cgtcgtccca cgacatgcgc ttccactcgt tcgagccggg tgcgcggtac tcggggtaca
      961 gcagccggct cgggctgttg acgatgtcca gcaggctcgc gcccttgggg cacagcgtgc
     1021 cgcggttcac cgggtggtcg gggtcgccct cgacgtggat caccgagcgc ttcgcgttct
     1081 tggcgttgtc gccgagcgaa tacatcagga tgccgcagcc gaccgagcag taggtgcagg
     1141 tctggcgcgt gaccgtggtg gcggcgagct tgtactgacg cacctcggcc agcgcggtcg
     1201 ccggcgagaa gcccatgagc gccaagctcg agcccgccag ggtggagcca gtcactttca
     1261 tgaactggcg acgcgagagt ttcatgagag gcctcgtcgt ggtgggtgat gggaatgtgc
     1321 gcggtgccag gcgcggccgg cagcgcttgg attcgacctc gcgcccgaca cgcacatgcg
     1381 ctcaagcgca catgcccgcc attaaccgcc accttcggag gcgatgcaat acgggtcaat
     1441 cctgagacaa cttgtcgccc gcagcgcgac aacctgtcgc gccgccacgc cgcgccgcag
     1501 cagggttttg gcagcgcgca gcgtttcgct tcggcgctgc gccggactcc attttcccgt
     1561 ctcgcgcaag gagggatggg ctttgatcat aggagatgta gttcatatga gagacggccg
     1621 acgtgcgcgt cggccggggg cagataatcc ggctgcgatg aacccactgc cactgcatcc
     1681 accctcggtc gaccggctgc tcgacacgcc cgcgctgcgc gcgctgatcg agcagcacgg
     1741 tcgcaccggc gtcgtgcagg cggtgcgcga gcagctcgac gcctggcgcg cggcggccgc
     1801 ctccggcttc gacggcgcac gcttcgagca ggcgtgccgc gagcggctcg acgcgcgcgc
     1861 cgcatcgagc ctgaggccgg tgttcaacct caccggcacc gtgctgcaca cgaacctggg
     1921 tcgcgccgtg ttgccgcccg aagcagtcga cgcggtggcc gccgtgatgc gccagccatc
     1981 gaacctcgag tacgagctcg gcggcggcgc ccgcggcgac cgcgacgatc acgtcgaggg
     2041 cctgctgcgc gagctgacag gcgcgcaggc cgccaccgcc gtcaacaaca acgccgccgc
     2101 ggtgctgctg atgctgaaca cgctcgcgct cggcaaggag gtgccggtct cgcgcggcga
     2161 gctgatcgag atcggcggcg cgttccgcat cccggacatc atgcggcgcg ccggcgcgaa
     2221 gctggtcgag gtcggcacga ccaatcgcac ccacctgcgc gactacgccg aagcgatcgg
     2281 ccccgacacc gcggcgctga tgaaggtgca cacgagcaac tacgtcatcg aaggcttcac
     2341 cgccgcggtg gccgaggccg agctggccgc gctcgcgcgc gagcgcgggt tgccgttgat
     2401 cgtcgatctc ggcagcggct cgctggtcga tctgacgcgc tggggcctgt cgcccgagcc
     2461 gacgccgcgc caggcgctgg ccagcggcgc caacctcgtc agcttcagcg gcgacaagct
     2521 gctcggcggg ccgcaggcgg ggctgatcgt cggcgacgcc gatctcgtcg cacgcatcaa
     2581 gcgcaacccg ctgaagcgcg cgctgcggct cgacaagatg acgctcgccg cgctcgaagc
     2641 cgtgctgaag ctgtaccgcg acccggatcg cctcgcgcag cgcctgccga cgctgcgcgc
     2701 gctgacccgg ccggtcgccg agttgcaggc cctcgccgaa cgcctcgcgc cggcgctgcg
     2761 cgcggcgctc gggccgctcg cgcaggtgca ggtgcgcgca tgccgcagcc agatcggttc
     2821 gggctcgctg ccggtcgatc ggctcgcgag tatctgcctc ggcgtcggcg cggccgcagg
     2881 cggatcgggc ggctatcccg acaagctcgc cgccgcgttc cgcgcgctgc cggtgccggt
     2941 gatcgggcgc atcgaggaag gcgagttgcg cctcgacctg cgctgcctcg acgacgaggc
     3001 gagcttcgtc gcgcaactga cggcgctggt ggcctgaccc tggaggcgcc tcaccgctcg
     3061 gggccgcgcg cgacgcggat gatcgtgctg cccatcatga cgttgttcgg caccacgacc
     3121 tcggccttct ggtcgtcgac gaggatggtg aagcccagcg agatgacctg aaccgtggcc
     3181 gtgacgacac ccgcgggaga gtcgacgcgg atcgtgtcgc cgacgcgaac ctgccggtac
     3241 agcaccagca agaagccggc gatcagacaa ccgagcgcgt ccttggcggc gaggccgacg
     3301 accaccgaga agacgctggc tccggcgagc aacgcggtgc cgaagacgcg cagctccggc
     3361 acgaggtggg cgcacagcgc gaaggcgatc aggtaggtca gcagttgcgc cagcgaactg
     3421 acgaatcgca gcgccgtgac atccgacagg tgctgcccca cgcggcgcgc cgttctgcgg
     3481 atcaacacga ccacgaccga tgcgagcccg aagatcagcg cgcccagcag cgcggccccg
     3541 acgaacgaat tccggttcag cgcgtctcga gccacatcgc cgagatggtc cattcctgct
     3601 cccttgtctt ggctctcgag tatcgcttca gggccgcttc ttgcgttcct tcagcgtcgg
     3661 aaacatcgcc tgcagatcgc cgaccacgcc ggtcgattcg tcggcgtggt aacccggcag
     3721 ccgattcacc gtcaccttga actcgtcgcc ctggacg
//
LOCUS       DASRDG010000623         4221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_804394, whole genome shotgun sequence.
ACCESSION   DASRDG010000623 DASRDG010000000
VERSION     DASRDG010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804394"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFA35_10440"
     CDS             complement(<1..1034)
                     /locus_tag="VFA35_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZT56635.1"
                     /translation="MATPNPLPFRPTNPGLTTLGLSLAVFMQVLDTTIANVSLPTIAG
                     NLGASADQATWVITSFAVSNAIALPLTGWLTRRFGEVKLFVAATLLFCVASFLCGIAQ
                     SMTMLILFRALQGAVAGPMYPVTQSLLLSIYPPEKRSQALALLIMVTIVAPIAGPILG
                     GWITDSYSWPWIFFVNVPVGIFAALIVYAQMGQRSEQTMRPKMDYVGLATLIIGVGAL
                     QILLDKGNDEDWFQSNFIILMTIVSVVSLAIFLIWELTDKHPVVDLSLFKRRNFWSGA
                     LATAVAYGLFFGNVVILPLWLQQWMGYTATQAGMIMAPVGLLAIFFSPVVGLTVGKID
                     PRRYATFSFLV"
     gene            complement(1210..2403)
                     /locus_tag="VFA35_10445"
     CDS             complement(1210..2403)
                     /locus_tag="VFA35_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZT56636.1"
                     /translation="MSTPNPTTETAAPTNGKRKLLLRLVLLIVVVGAIGFGIWYYTIG
                     RWYEGTDDAYVNGNVVQITPQVAGTVVRINADDGNLVHKGDVLVEIDGSDARIALQQA
                     EAQLARTVRQVRGLYSNAEGGQAIVAAQKVSLDRARADYARRQGLVATGAISAEELAH
                     ARDALATAESSLASTRQQLSTTTALVDETVIAKHPDVLAASAALRVAYLNDARSKIYA
                     PVEGYVAKRSVQVGQRLQPGSPLMAVVPLHQVWIDANFKETQLAHMRIGQPVALKSDL
                     YGSKVEFKGKVQSLGVGTGSAFSLLPAQNATGNWIKIVQRIPVRIELDAKELDKNPLR
                     IGLSMAADVNLHDQSGPVLAQQPPKEPAFITDVYQGQMQGAQADIDRVIHDNAARTSA
                     DVATK"
     gene            complement(2504..3952)
                     /locus_tag="VFA35_10450"
     CDS             complement(2504..3952)
                     /locus_tag="VFA35_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZT56637.1"
                     /translation="MRTLALFPLLLTAAITLAGCAGTGGLHTDGTPTDAATLHSERSF
                     ARIKTTPAAWPASDWWTQLGDAQLDALIAEALKDNPDLASADARAKEAQSQVDAQNAK
                     RLPTVNAAGSVAGVYLPTTAVPAPVGGSFGTYPAVYASFNWSLDLWGGKRAAWEAALG
                     QARAAEIDAHAARLALSVNVARAYVQLGYAFAEKDVADAQLKRASASHALVKQRVAAG
                     IDNRMQIKQAEAEVASAEQQVAVTDEAIDAARIALAVLLGKGPDRGLDIAHPKSLQPA
                     ALVLPPNLNADLIGRRADLVAARWRVEAATKSIAAVKTEFLPNIGLSAFAGLTSSSFD
                     NLLSLPARFYMVQPAISLPIFDGGRRRADLHNANAGYDLAVAHYNSTLVGAVNDVADK
                     LANLGSLQTQSAAQQRAVDAARTAYDLSQQRYKAGIGSYLDTLSVQQQLLIAEQRAAA
                     LAAQQVDTSVRLVQALGGGFNAGDGVPPVAER"
     gene            complement(3979..>4221)
                     /locus_tag="VFA35_10455"
     CDS             complement(3979..>4221)
                     /locus_tag="VFA35_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZT56638.1"
                     /translation="AMTRLLDQLEDKGYVERKPHAQDRRALTIELTPAGKELWRKMSQ
                     FIEHAQDLALRDLKPTDRAKLFDLLKRVRDTLDETP"
BASE COUNT          666 a         1293 c         1498 g          764 t
ORIGIN      
        1 accaggaacg aaaaggtcgc gtaccggcgc gggtcgatct tgcccacggt gagtccgacg
       61 acgggcgaga agaagatcgc caacagcccg accggcgcca tgatcatgcc ggcctgcgtt
      121 gcggtatagc ccatccactg ctgcaaccac agcggcagga tcaccacgtt gccgaagaac
      181 aggccgtagg ccaccgcggt ggccagcgcg cccgaccaga aattgcggcg cttgaacagc
      241 gacaggtcga ccaccgggtg cttgtcggtc agctcccaga tcaggaagat ggcgaggctg
      301 acgaccgaga cgatcgtcat caggatgatg aagttcgatt ggaaccagtc ctcgtcgttg
      361 cccttgtcga gcaggatctg cagcgcgccg acgccgatga tcaaggtggc gaggccgacg
      421 tagtccatct ttggccgcat cgtctgctcg ctgcgctggc ccatctgcgc gtagacgatg
      481 agcgcggcga agatgccgac cggcacgttg acgaaaaaga tccacggcca ggaatagctg
      541 tcggtgatcc agccgccgag gatcgggccg gcaatcggcg cgacgatggt gaccatgatc
      601 aacagggcca gcgcctgact gcgtttctcg ggcggataaa tcgacagcag caggctctgc
      661 gtgaccggat acatcggtcc ggcgacggca ccctgcagcg cgcggaacag gatcagcatc
      721 gtcatgctct gcgcgatgcc gcagaggaaa gaagcaacgc agaacagcag cgtcgcggcg
      781 acgaacagct tcacttcgcc gaagcgtctg gtcagccagc cggtcagcgg cagggcgatg
      841 gcgttgctga ccgcgaacga ggtgatgacc caggtcgcct ggtccgcgct cgcgccgagg
      901 ttgcccgcga tcgtcggcag cgacacgttg gcgatcgtcg tgtcgagcac ctgcatgaac
      961 acggcgagcg aaaggccgag cgtggtcagg ccggggtttg tggggcggaa tggcaaggga
     1021 ttcggggtgg ccattgttgg aagagcctgt ttgccgcaga ttgcgcgact ggagcagata
     1081 tcggatcgta gtggttcgat tttcgcgcgc ctcgcgcgaa agaaatcgat tcgtatcgaa
     1141 cccggatttt gatctttttg tcgaaaaagc gggcgcgacc gatctgatca aatcagcacg
     1201 ccccctaggt cacttcgtgg ctacgtcggc actggtgcgc gccgcattgt cgtgaatgac
     1261 gcggtcgatg tcggcttgcg cgccttgcat ctggccttgg tagacgtcgg tgatgaacgc
     1321 gggctccttc ggcggctgct gggcgagcac cggcccgctc tgatcgtgca ggttcacgtc
     1381 cgccgccatc gacaggccga tgcgcagcgg attcttgtcg agttccttgg cgtccaattc
     1441 gatgcgcacg gggatgcgct gcacgatctt gatccagttg ccggtcgcgt tctgcgcagg
     1501 cagcagcgag aacgcgctgc cggtgccgac gccgaggctc tgtaccttgc ccttgaactc
     1561 gaccttggaa ccgtacaggt cggacttcag cgccaccggt tgaccgatgc gcatgtgcgc
     1621 gagctgggtt tccttgaagt tcgcatcgat ccagacctgg tgcagcggca ccacggccat
     1681 cagcggcgaa ccgggctgca ggcgctggcc gacctgcacg ctgcgcttgg cgacgtagcc
     1741 ttcgaccggc gcgtagatct tcgagcgtgc gtcgttgaga taggcgacgc gcagcgcggc
     1801 actcgccgca agcacgtcgg gatgcttggc gatcacggtc tcgtcgacga gcgccgtggt
     1861 cgtgctcaac tgttgccggg tcgaggccag cgaactttcc gcggtggcga gcgcatcgcg
     1921 cgcgtgcgcg agctcctcgg ccgagatcgc gccggtggct acgaggccct ggcggcgcgc
     1981 gtagtcggcg cgcgcgcggt cgagcgagac cttctgcgcg gcgacgatgg cctggccacc
     2041 ctcggcattg ctgtacaagc cgcgtacctg gcgcacggtg cgcgcgagct gggcttcggc
     2101 ctgctgcagg gcgatgcgtg cgtcgctgcc gtcgatctcg acgagcacgt cgcccttgtg
     2161 cacgaggttg ccgtcgtcgg cgttgatcct gaccacggtg ccggcgacct gcggcgtgat
     2221 ctgcacgacg ttgccgttga cgtaggcgtc gtcggtgcct tcgtaccaac ggccgatcgt
     2281 gtagtaccag atgccgaagc cgatcgcgcc gacgacgacg atcaggagga cgaggcgcag
     2341 cagcagtttg cgtttgccgt tggtcggcgc ggcggtttcg gtggtggggt ttggggtgct
     2401 catgactgta tacctttgca aaagtatctt ttggccgttt cttttttctc gtcatcccgg
     2461 cggctttcaa cagccgaagg gctggtcaag ccgggatgac gagctatctt tctgccactg
     2521 gcggcacgcc gtcgcccgca ttgaagccac caccgagcgc ctgcacgagg cgcaccgaag
     2581 tgtcgacctg ctgcgcggcg agcgcggcgg cgcgctgctc tgcgatgagc agttgctgct
     2641 gcacgctcag tgtgtcgagg tagctgccga tgccagcctt gtagcgttgc tgggaaaggt
     2701 cgtacgcggt gcgcgccgca tcgacggcgc gttgctgcgc ggcactttgc gtctgcagcg
     2761 agccgaggtt ggcgagcttg tcggcgacgt cgttgaccgc accgacgagg gtcgagttgt
     2821 agtgcgcgac ggcgaggtcg tagcccgcgt tggcattgtg gagatcggca cggcgacggc
     2881 cgccgtcgaa gatcggcagg ctgatcgccg gctgcaccat atagaagcgc gcaggcagcg
     2941 agagcaggtt gtcgaacgag gacgaggtca ggccggcgaa cgcggacagt ccgatgttcg
     3001 gcaggaactc ggtcttcacc gcggcgatgc tcttcgtcgc cgcctcgacg cgccagcgcg
     3061 cggcgacgag gtcggcgcga cgaccgatca gatcggcgtt caggttcggc ggcagcacga
     3121 gcgcggcagg ctgcagcgac ttcggatggg cgatgtcgag accgcggtcc gggcccttgc
     3181 cgagcagcac ggcgagcgcg atgcgcgcgg cgtcgatcgc ctcgtccgta acggcgacct
     3241 gttgttcggc gctggcgacc tcggcttcgg cctgcttgat ctgcatgcgg ttgtcgatgc
     3301 cggcggcgac gcgctgcttg accagcgcat gcgaggcgct tgcgcgtttg agctgcgcgt
     3361 cggcgacatc cttctcggcg aatgcatagc cgagttgcac ataggcgcgg gcgacgttga
     3421 ccgacagcgc gaggcgtgcg gcgtgcgcat cgatctcggc ggcacgcgcc tggccgagcg
     3481 ccgcttccca ggcggcgcgc ttgccgcccc agaggtcgag gctccagttg aaactcgcat
     3541 agaccgcggg atacgtgccg aacgagccgc cgaccggtgc gggcaccgcc gtcgtcggca
     3601 aatagacgcc ggcgacgctg cccgcggcgt tgaccgttgg caggcgcttg gcgttctgcg
     3661 catcgacctg gctttgcgct tctttggcgc gcgcgtcggc gctggccagg tcggggttgt
     3721 ccttgagcgc ttcggcgatc agtgcgtcga gttgcgcgtc gccgagttgc gtccaccagt
     3781 cggacgcggg ccacgcggcc ggcgtcgtct tgatcctggc gaagctgcgc tcgctgtgca
     3841 gcgttgccgc atcggtcggc gtgccgtcgg tgtgaaggcc gcccgtgccg gcgcagccgg
     3901 ccaacgtgat cgccgcggtg agcagaaggg gaaaaagggc gagggtacgc atggcgcggt
     3961 ccttttcgcg ggtggatttc atggggtttc gtcgagggtg tcgcgcacgc gcttgagcag
     4021 gtcgaagagc ttggcgcggt cggtcggttt caggtcgcgc agggccaggt cctgggcgtg
     4081 ctcgatgaac tggctcatct tgcgccacag ctccttgccg gccggggtga gttcgatcgt
     4141 cagcgcgcgg cgatcctgcg cgtgcggctt gcgctcgacg tagcccttgt cttcgagctg
     4201 atcgagcaga cgcgtcatcg c
//
LOCUS       DASRDG010000624         4523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_668400, whole genome shotgun sequence.
ACCESSION   DASRDG010000624 DASRDG010000000
VERSION     DASRDG010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668400"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..2107)
                     /locus_tag="VFA35_10460"
     CDS             complement(137..2107)
                     /locus_tag="VFA35_10460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZT56639.1"
                     /translation="MPAHRKTPAPFGSWPSPVTAELIAGATIRLGQIALCGDEIVWSE
                     GRPQEQGRNVLVRRRADGTLADLNPAPLNVRTRAHEYGGGAFVLLPDGSVVFSHYDDQ
                     RLYRVGAAGVVQPLTHDADQRYADALVDSARNRLIAVCEDHRTAESDGGGDAIATLVA
                     IALDSGAVTVLAQGHDFFASPALSPDGRRLAWIAWDHPNMPWDGCTLWLADVAADGTL
                     STPQPIAGGIDESIFQPQWSPRGELHFVSDRSGWWNLYRWRDDAVQALHPMDAEFGEP
                     QWAFGMSRYGFDARGHIVCTVSQHGVARLARLDTETGAFETLATPFCAIDSLRVGADF
                     AVFVGATPTQPSAVVRLDLRTLAHEVLRSASTARVAAAYLSIAAAISFPTEGGLEAHA
                     FFYPPTHPEACGPAGELPPLIVISHGGPTGATDAGFKWSYQYWTSRGFALVDVNYGGS
                     TGYGRAYRARLDGQWGVVDVDDAVNAARYLAARGAVDGERIAIRGGSAGGYTTLCALT
                     FRSFFKAGASHYGIGDLEALATDTHKFESRYLDRLVGPYPQAKALYRERSPVHHTARL
                     ARPMILFQGAEDKAVPPAQAQAMFDAVAAKGLPVAYLLFEGEQHGFRRAATIKRVFEA
                     ELCFYGKVFGFTPADAIEPVAIRNLKEHPLAP"
     gene            complement(2131..2397)
                     /locus_tag="VFA35_10465"
     CDS             complement(2131..2397)
                     /locus_tag="VFA35_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HZT56640.1"
                     /translation="MDDTPSPASAAPQARNPLHGITLEAMLTELVAHFGWPELGARVR
                     INSFNSDPSIASSLKFLRKTPWARAKVEGLYLFMRREQARAKRG"
     gene            2507..3046
                     /locus_tag="VFA35_10470"
     CDS             2507..3046
                     /locus_tag="VFA35_10470"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZT56641.1"
                     /translation="MKKIMAAVAATLAIAAPAWAALDIGEKAPDFTAPAALAGKQYSF
                     SLSQTLAKGPVVLYFFPAAFTTGCSAEAHEFAEATEKFAALGATVIGVSTDDIDTQLK
                     FSMQACQGKFAVASDAAKSVIKSYDAAMMIMPDFANRVSYVITPDRSIIYSYQSLNPA
                     KHVTNTLTALQNWEKTRKK"
     gene            complement(3063..3248)
                     /locus_tag="VFA35_10475"
     CDS             complement(3063..3248)
                     /locus_tag="VFA35_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HZT56642.1"
                     /translation="MFSVFQSIKNLFSVSANPAQDRDDQYLAESSDIYDLERRMRQID
                     SGRHHLYAIGAYGLLMR"
     gene            3381..>4523
                     /locus_tag="VFA35_10480"
     CDS             3381..>4523
                     /locus_tag="VFA35_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT56643.1"
                     /translation="MFQFFEKLVQPYPDAEPAPPPKRFFPFLWACTRGLRPYMIAMTL
                     CSAVIGAFEALLFAFLGRVVDWLGATTPARLWAEQGHNLLLLGAVLAASIGLVAVQSL
                     LKQQAMAGNFPMLLRWNFHRLMLAQSMHFYQDEFAGRISTKVMQTALAVRDAWMILAE
                     LLVFVVIYFVTIMAMLGSFDRWLLAPFSAWLALYCAALWYFVPRLGKVAKAQADARSM
                     MTGRVTDAYTNIATVKLFSHSKREAGYARAAMQEFLVTVQSQMRLVTGFEIVNQALSM
                     ALIAATTGAALWLWTQGQAGVGALAAATAMALRLNGISHWVMWEMASLFEHIGTVQDG
                     IATLARPRQVVDRADARPLEVSRGEIRFEHVRFAYGGRNVVIDDLSL"
BASE COUNT          672 a         1530 c         1594 g          727 t
ORIGIN      
        1 tcaacctcgg cctcgttgcg gtagaccacg atgtcctgcc cttgccaggc ggtgcgccac
       61 gacgatctca ttcacgttcc tccatcagaa cggtgaccgt aacgtgtacg cgcgcatctg
      121 cgtgtcagca gacgcgtcaa ggtgcaagcg ggtgttcctt caggttgcga atggcgaccg
      181 gctcgatcgc atcggccggc gtgaaaccga acaccttgcc gtagaagcac agctcggctt
      241 cgaacacgcg cttgatggtt gcggcgcgcc ggaagccgtg ctgctcgccc tcgaacagca
      301 ggtaggcgac cggcagcccc ttcgcggcga ccgcgtcgaa catcgcttgc gcctgcgcgg
      361 gcggcacggc cttgtcctcg gcgccctgaa acaggatcat cggcctggcg agccgggcgg
      421 tgtggtggac cggcgagcgc tcgcggtaca gcgccttcgc ctgcggatac ggcccgacca
      481 gccggtcgag gtagcgcgac tcgaacttgt gggtgtcggt cgcgagcgct tccaggtcgc
      541 cgatgccgta gtggctcgcg ccggccttga agaagctgcg gaaggtcagc gcgcacagcg
      601 tggtgtagcc gcccgcgctg ccgccgcgga tcgcgatgcg ctcgccgtcg accgcgccgc
      661 gcgccgccag gtaacgcgcc gcattgaccg cgtcgtcgac gtcgaccacg ccccattgcc
      721 cgtcgagccg cgcgcggtag gcgcggccgt agccggtgct gccgccgtag ttcacgtcga
      781 ccagcgcgaa gccgcggctg gtccagtact ggtacgacca cttgaacccg gcgtcggtcg
      841 cgccggtggg gccgccgtgg ctgatcacga tcagcggcgg cagttcgccc gcggggccgc
      901 aggcctcggg gtgggtcggc gggtagaaga acgcgtgcgc ctcgagcccg ccttcggtcg
      961 ggaagctgat cgccgcggcg atcgagaggt aggcggcagc tacgcgcgcg gtgctggcgc
     1021 tgcgcagcac ctcgtgcgcg agcgtgcgca ggtccagccg cacgaccgcg ctcggctgcg
     1081 tcggtgtggc gccgacgaag accgcgaagt cggcgccgac gcgcagcgag tcgatcgcgc
     1141 agaacggcgt ggccagcgtc tcgaaggcgc cggtctcggt gtcgagccgg gcaagccgcg
     1201 ccacgccgtg ctggctgacc gtgcagacga tgtgcccgcg tgcgtcgaag ccgtagcggc
     1261 tcatgccgaa agcccattgc ggctcgccga actcggcgtc catcgggtgc agcgcctgca
     1321 ccgcgtcgtc gcgccaccgg tacaggttcc accagccgct gcggtcgctg acgaagtgca
     1381 gctcgccgcg cggcgaccac tgcggctgga agatcgattc gtcgatgccg ccggcgatcg
     1441 gctgcggcgt cgacagcgtg ccgtcggcgg ccacatcggc gagccagagc gtgcagccat
     1501 cccacggcat gttcgggtgg tcccaggcga tccacgcgag ccggcggcca tcggggctga
     1561 gggcggggct cgcgaagaag tcgtggcctt gcgcgagcac cgtgacggcg ccgctgtcga
     1621 gcgcgatcgc caccagcgtc gcgatcgcat cgccgccgcc atcactttcg gcggtgcggt
     1681 ggtcttcgca caccgcgatc agccggttgc gggcgctgtc gacgagcgca tcggcgtagc
     1741 gctggtccgc gtcgtgcgtc agcggctgga cgacgccggc agcaccgacc cggtagagcc
     1801 gctggtcgtc gtagtgactg aacaccacgc tgccatcggg caacagcacg aacgcaccgc
     1861 cgccgtactc gtgcgcgcgg gtgcgcacgt tgagcggcgc cgggttcagg tcggccagcg
     1921 tgccgtcggc acggcggcgc acgagcacgt tgcggccctg ctcctgtggc cggccttcgc
     1981 tccagacgat ctcgtcgccg cacagcgcga tctggccgag gcggatcgtc gcgccggcga
     2041 tcagctcggc ggtgaccgga gagggccagc tgccgaacgg cgcgggcgtc ttgcggtgtg
     2101 cgggcatggc ggtcgctcgg gttgcggcgt tcagccgcgc ttcgcgcgcg cctgttcgcg
     2161 ccgcatgaac aggtacagcc cttcgacctt cgcgcgcgcc cacggcgtct tgcgcaggaa
     2221 cttcaggctc gacgcgatgc tcgggtcgct gttgaagctg ttgatccgga cgcgcgcacc
     2281 gagctcgggc cagccgaagt gcgcgaccag ctcggtcagc atcgcttcga gcgtgatgcc
     2341 gtgcagcggg ttgcgcgctt gtggcgcggc cgaggcaggc gagggcgtgt cgtccatgtc
     2401 ggcggatcat atcggggccg ccgcctacag ccagcagggc cagtcgggaa catgccaacg
     2461 ggccggaacg cttctggcaa gatcgcgcgg tgtcaaggag caggcgatga agaagatcat
     2521 ggcagcagtg gcagcgaccc tggcgatcgc cgcgccggcc tgggccgcgc tcgacatcgg
     2581 cgagaaggcg cccgacttca ccgccccggc cgcgctcgcc ggcaagcagt acagcttttc
     2641 gctgagccag acgctggcga agggcccggt ggtgctgtat ttcttcccgg ccgcgttcac
     2701 gacgggttgc tcggccgagg cacacgagtt cgccgaagcc accgagaagt tcgctgcgct
     2761 cggtgcgacc gtgatcggcg tctcgaccga cgacatcgac acgcagctca agttttcgat
     2821 gcaggcctgc cagggcaagt tcgcggtggc ctccgacgcg gccaagtccg tgatcaagtc
     2881 ctacgacgcg gcgatgatga tcatgccgga cttcgcgaac cgcgtgtcct acgtcatcac
     2941 gcccgaccgc tcgatcatct acagctacca gagcctgaac ccggccaagc atgtgacgaa
     3001 cacgctgacc gcgctgcaga actgggagaa gacgcgcaag aagtagcgcg ccgaatcact
     3061 gttcagcgca tcagcaggcc gtaggcgccg atcgcgtaca ggtggtggcg gccggagtcg
     3121 atctggcgca tgcggcgctc gaggtcgtag atgtccgacg attcggccag gtactggtcg
     3181 tcgcgatcct gggccgggtt ggccgacacg ctgaacaggt tcttgatcga ctggaaaacg
     3241 gaaaacatgg gggtctttgg agtttctagg cttggcatta tgcgggaaaa ccctaggttt
     3301 tgcccgattc gccggcgagg ccttgaggag aggcgcctct aatcccgccg ccccgccgaa
     3361 cccgatccgg agatccccgc gtgttccagt tcttcgaaaa gctcgtccag ccttacccgg
     3421 acgccgagcc cgcgccgccg cccaagcgct tcttcccgtt cctgtgggcc tgcacgcgcg
     3481 gcctgcggcc ctacatgatc gcgatgacct tgtgcagcgc ggtcatcggc gcgttcgagg
     3541 ccttgctgtt cgcgtttctc ggccgcgtgg tcgactggct cggcgcgacc acgccggcgc
     3601 gcctgtgggc cgagcagggg cacaacctgc tcctgctcgg cgccgtgctg gccgccagca
     3661 tcgggctcgt cgcggtgcaa tcgctgttga agcagcaggc gatggccggc aacttcccga
     3721 tgctgctgcg ctggaacttc caccgcctga tgctcgcgca gagcatgcac ttctaccagg
     3781 acgagttcgc cggccgcatc tcgaccaagg tgatgcagac cgcgctggcg gtgcgcgacg
     3841 cctggatgat cctggccgag ctgctcgtgt tcgtggtgat ctacttcgtg acgatcatgg
     3901 cgatgctcgg cagcttcgac cgctggctgc tggcgccgtt cagcgcgtgg ctggcgctgt
     3961 actgcgcggc gctgtggtac ttcgtgccgc gcctgggaaa agttgcgaag gcgcaggccg
     4021 atgcgcgctc gatgatgacc gggcgcgtca ccgacgccta caccaacatc gcgaccgtca
     4081 aactgttctc gcattccaag cgcgaggccg gctacgcgcg cgcggcgatg caggagtttc
     4141 tcgtcacggt gcagtcgcag atgcggctcg tgaccggctt cgagatcgtc aaccaggcgc
     4201 tgagcatggc gctgatcgcg gccacgaccg gcgccgcgct gtggctgtgg acgcaagggc
     4261 aggccggcgt cggcgcgctc gctgccgcga ccgcgatggc gctgcgcctg aacggcatct
     4321 cgcactgggt gatgtgggag atggcgtcgc tgttcgagca catcggcacg gtgcaggacg
     4381 gcattgccac gctcgcgcgc ccgcgccagg tcgtcgaccg cgccgacgcc cgcccgctcg
     4441 aggtgagccg cggcgagatc cgcttcgagc acgtccgctt cgcctacggc ggccgcaacg
     4501 tggtgatcga cgacctgtcg ctg
//
LOCUS       DASRDG010000625         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1480279, whole genome shotgun sequence.
ACCESSION   DASRDG010000625 DASRDG010000000
VERSION     DASRDG010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480279"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..81)
                     /locus_tag="VFA35_10485"
     CDS             complement(<1..81)
                     /locus_tag="VFA35_10485"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HZT56644.1"
                     /translation="MAKLTTHVLDTANGCPAAGMAVALYRL"
     gene            188..934
                     /locus_tag="VFA35_10490"
     CDS             188..934
                     /locus_tag="VFA35_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZT56645.1"
                     /translation="MASSAPKLQVIRTTKANAARAADPATSENLTQRIAEAITTAIIE
                     RRLMPGTKLAEQKIADIFKVSRTLVRQALMQLSRDHLITLEPARGARVAEPSIEEAKQ
                     VFEARNMLEAAMIRRAAAELTDAQIAELRAHLKAEADALRRTDVSGRTRLLADFHVVI
                     AQMLGNAVLAGMLGDLVSRCSLIALMYQSAHSAEHSAGEHVAIVDALARRDARAAVRL
                     METHLHNVERNLRLDPRAPDLGSVLLPVGR"
     gene            950..1873
                     /gene="puuE"
                     /locus_tag="VFA35_10495"
     CDS             950..1873
                     /gene="puuE"
                     /locus_tag="VFA35_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HZT56646.1"
                     /translation="MTKDYPRDLIGYGRNPPQANWPGNARIAVQFVLNYEEGAENCVL
                     HGDPSSETFLSEIIGAQAFETRHLSMESMYEYGSRVGAWRILREFEKRGLPLTVFGVS
                     MALHRNPELTRAFLDLNHEIACHSWRWIHYQHMSETRERRHIEIGTQIIKDLTQGQWP
                     LGWYTGRDSPNTKRLVADHGGYEYDSDYYGDDLPFWMPVEKSDGTTIDRLIVPYTLDT
                     NDMRFASMQGFNTGDHFYQYLKDAFDVLYEEGAQTPKMLSIGMHCRILGRPGRFKALQ
                     RFLDHVQQHERVWICRRIDIARHWAATHPAA"
     gene            1893..>2106
                     /locus_tag="VFA35_10500"
     CDS             1893..>2106
                     /locus_tag="VFA35_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HZT56647.1"
                     /translation="MASSLTLDALNRAAPAQFTALLDGVYEHSPWIAEAACAQRPFAS
                     LAQLKYALVQVLRDAGRGAQIALIRAH"
BASE COUNT          380 a          746 c          676 g          304 t
ORIGIN      
        1 gagccgataa agcgcgacgg ccatgccggc ggccgggcag ccgttggcgg tgtcgagcac
       61 gtgggtggtg agcttggcca tggatggcgt cctcgcgcgg tgaacaagaa agtgcttgat
      121 gcgtggtcaa aagtgtatac actttgtctc acaaagcaag cccgctccga tctttgcgcc
      181 ccgccacatg gcctcctccg caccgaaact gcaagtcatc cgcaccacga aggcgaatgc
      241 cgcgcgcgcg gccgacccgg caacgagcga gaacctcacg cagcgcatcg ccgaggcgat
      301 caccaccgcg atcatcgagc gccggctgat gccgggcacc aagctggccg agcagaagat
      361 cgccgacatc ttcaaggtct cgcgcacgct ggtgcgccag gcgctgatgc agctcagccg
      421 cgatcacctg atcacgctcg agccggcgcg cggcgcacgc gtggccgagc cgagcatcga
      481 ggaggcgaag caggtcttcg aggcccgcaa catgctcgag gcggcgatga tcaggcgcgc
      541 cgccgccgaa ctcaccgatg cgcagatcgc cgagctgcgc gcgcacctca aggccgaagc
      601 cgacgcgctg cggcgcaccg acgtgtcggg ccgcacccgg ctgctcgccg actttcacgt
      661 cgtgatcgcg cagatgctgg gcaacgcggt gctggccggc atgctcggcg acctggtctc
      721 gcgctgctcg ctgatcgcgc tgatgtacca gtcggcgcac tcggccgagc actcggccgg
      781 cgagcatgtc gcgatcgtcg acgcactcgc ccggcgcgac gcgcgcgccg cggtcaggct
      841 gatggaaacg cacttgcaca atgtcgagcg caacctgcgg ctcgacccgc gcgcgcccga
      901 tctgggctcg gtcttgctac cggtcgggcg ctgagagaac accacgatga tgaccaaaga
      961 ctatccgcgc gacctgatcg gctacggccg caacccgccg caggcgaact ggcccggcaa
     1021 cgcccggatc gccgtgcagt tcgtgctgaa ctacgaggag ggcgccgaga actgcgtgct
     1081 gcacggcgac ccgtcgtccg agacctttct gtccgagatc atcggcgcgc aggctttcga
     1141 gacccggcac ctgtcgatgg agtcgatgta cgagtacggc tcgcgcgtcg gcgcctggcg
     1201 catcctgcgc gagttcgaga agcgcggcct accgctcacc gtgttcggcg tctcgatggc
     1261 gctgcaccgc aaccccgagc tgacccgagc cttcctcgac ctgaaccacg agatcgcctg
     1321 ccactcgtgg cgctggatcc actaccagca catgagcgag acgcgcgaac gccgccacat
     1381 cgagatcggc acgcagatca tcaaggactt gacccagggc cagtggccgc tcggctggta
     1441 caccgggcgc gacagcccga acaccaagcg actcgtggcc gaccacggcg gctacgagta
     1501 cgacagcgac tactacggcg acgacctgcc gttctggatg ccggtcgaga agagcgacgg
     1561 cacgacgatc gaccgcctga tcgtgcccta cacgctcgac accaacgaca tgcgcttcgc
     1621 gtcgatgcag ggcttcaata ccggcgatca cttctatcag tacctgaagg acgcgttcga
     1681 cgtgctgtac gaagaaggcg cgcagacgcc caagatgctg tcgatcggca tgcactgtcg
     1741 catcctcggc cggcccggcc gcttcaaggc gctgcagcgt ttcctcgacc acgtgcagca
     1801 gcacgagcgg gtgtggatct gcaggcgcat cgacatcgcg cgccactggg ccgccacgca
     1861 ccctgccgcc tgaaaaagta cccggagaca cgatggcctc ttccctcacg ctcgatgcac
     1921 tcaaccgcgc ggcaccggcg cagttcacgg cgctgcttga cggcgtctac gaacactcgc
     1981 cgtggatcgc cgaggccgcc tgcgcgcagc gcccgttcgc cagcctggcg cagctgaagt
     2041 acgcgctggt gcaggtgctg cgcgacgccg gccgcggcgc gcagatcgcg ctgatccgcg
     2101 cccacc
//
LOCUS       DASRDG010000626         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_457710, whole genome shotgun sequence.
ACCESSION   DASRDG010000626 DASRDG010000000
VERSION     DASRDG010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457710"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFA35_10505"
     CDS             <1..264
                     /locus_tag="VFA35_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family drug resistance efflux
                     transporter"
                     /protein_id="HZT56648.1"
                     /translation="PLGIYAFGWALMVPVVTLMVLDLVPERRGMASSFQACIGSIANG
                     LVAGVIAPLVMHSTIWLAVASLLMMSVGVFAWIWVKPRVTERH"
     gene            318..890
                     /locus_tag="VFA35_10510"
     CDS             318..890
                     /locus_tag="VFA35_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HZT56649.1"
                     /translation="MTLFIIGLVLFLGVHSVAIVAPAWRDAQAARSPGAWKGVYTLIS
                     IAGFALLIYGYGLARQSAVVVWTPPTAMRHIALLLMLPVFPLLLAAYLPGRIKTAAKH
                     PMLLAVKFWALAHLLANGTLTDVILFGAFLAWAVFDRIAVSKRAVPRAIPGAPAAAIN
                     DAIALVGGLAIYALFVLWAHRWLIGVSPLG"
     gene            1029..1214
                     /locus_tag="VFA35_10515"
     CDS             1029..1214
                     /locus_tag="VFA35_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZT56650.1"
                     /translation="MNKDQVKGRVEQVKGSIKEAAGKVVGNEKLEAEGKVDKAAGKTQ
                     AVYGDLKEDVKDAVKKP"
     gene            complement(1218..>2355)
                     /locus_tag="VFA35_10520"
     CDS             complement(1218..>2355)
                     /locus_tag="VFA35_10520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HZT56651.1"
                     /translation="QTILDASNAVIALAPQRFGKQLWSDAASSERPSLITVRDESAQA
                     AWVADQVLRHRERGIALKGQVALFRSSHHSAALELELTRRNIPFVKFGGLKFLEAAHV
                     KDVLAVLRWAQNLRSRMAGLRVAQLVPGIGTASAKRLLDAIASAADPVDALHAFKPPP
                     AALADWSAWLATFDALRADAHWPADLERANAWYRPQLRRLHDDAAIREGDLAQLARIA
                     SGYTSRASFLAELTLDPPEATSDESGPPLRDEDYMILSTIHSAKGQEWKAVFVLNAVD
                     GCIPSDMATGSAIEIEEERRLLYVAMTRARQHLHLLVPQRFYVTQQAEFGDKHLYGAL
                     TRFIPPAIAHCFDARSEEAPDDGSPALLPNLTLDVAARVGSMWS"
BASE COUNT          359 a          794 c          811 g          391 t
ORIGIN      
        1 ccgctgggca tctacgcgtt cggctgggcg ctgatggtgc ccgtggtcac gctgatggtg
       61 ctcgacctgg ttcccgagcg acgcggcatg gcctcgtcgt tccaggcctg catcggcagc
      121 attgcgaacg gcctcgtcgc cggcgtgatc gcgccgctgg tgatgcactc gacgatctgg
      181 ctcgcggtgg cctcgctgct gatgatgagc gtcggcgttt tcgcctggat atgggtcaag
      241 ccgcgggtta ccgagcggca ctgaattgcc tgcgcaatcg aaccgataga ctggccgctc
      301 catcgaagga gcgcgtcatg accctgttca tcatcggcct cgtgctgttc ctcggcgtcc
      361 attcggtcgc gatcgtcgcg ccggcgtggc gcgacgcgca ggccgcgcgc agccccggcg
      421 cctggaaggg tgtctacacg ctgatctcga tcgccggctt cgcgctgctg atctacggct
      481 acggcctggc gcgccagtcg gccgtcgtcg tgtggacgcc gccgaccgcg atgcgccaca
      541 ttgcattgct gctgatgctg ccggtgttcc cgctgctgct ggccgcctac ctgccggggc
      601 gcatcaagac cgcggccaag cacccgatgc tgctcgcggt gaagttctgg gcgctcgcgc
      661 acctgctcgc gaacggtacg ctgaccgacg tgatcctgtt cggcgcgttc cttgcctggg
      721 cggtgttcga ccgcatcgcg gtgagcaagc gcgccgtgcc gcgcgcgatt ccgggcgcgc
      781 cggcggcggc gatcaacgac gcgatcgcac tggtcggtgg cctggcgatc tatgcgctgt
      841 tcgtgctgtg ggcgcatcgc tggctgatcg gcgtctcgcc gctgggttga atccgcgtgc
      901 gcggcgtcct acagcgggtg cgcaccgcgc ccgacagacc ttgaactgcc ccttcggcac
      961 agtgacctca tcgatcggaa gcagatcgat gccgggccac tctcttcact cagtgcaaag
     1021 gaatcagcat gaacaaggac caggtcaagg gccgcgtcga acaggtcaag ggcagcatca
     1081 aggaagccgc gggcaaggtc gtcggcaacg agaagctgga agccgaaggc aaggtcgaca
     1141 aggcagcggg caagacccag gccgtctatg gcgacctgaa ggaagacgtc aaggacgccg
     1201 tcaagaagcc ctgagcatca cgaccacatc gatccgacgc gcgcggccac atcgagcgtc
     1261 agattcggca ggagcgccgg cgagccatcg tccggcgctt cttcgcttcg tgcgtcgaag
     1321 caatgcgcga tcgccggcgg gatgaagcgc gtcaacgcgc catacagatg cttgtcgccg
     1381 aactcggcct gctgcgtgac gtagaagcgt tgcggcacga gcagatgtag atgctggcgc
     1441 gcgcgcgtca tcgcgacgta gagcaggcgc cgctcctcct cgatctcgat cgcgctgccg
     1501 gtcgccatgt cagacgggat gcagccgtcg accgcgttca gcacgaacac cgctttccac
     1561 tcctggcctt tcgcggaatg gatcgtcgac aggatcatgt agtcctcgtc gcgcaacggc
     1621 gggccggatt cgtcgctcgt cgcctcgggc gggtcgagcg tcagttcggc gaggaacgac
     1681 gcgcgcgacg tgtagcccga cgcgatgcgc gcgagctgcg ccaggtcacc ttcgcggatc
     1741 gcggcgtcgt cgtgcaggcg acgcagctgc ggccggtacc aggcgttggc gcgttccagg
     1801 tcggcgggcc agtgcgcgtc ggcgcgcagc gcatcgaagg tcgcgagcca cgccgaccaa
     1861 tcggccaacg cggccggcgg tggcttgaac gcatgcagcg cgtcgaccgg gtcggccgcg
     1921 ctcgcgatcg catcgagcag ccgcttcgcg ctcgccgtgc cgatgcccgg caccagctgc
     1981 gcgacgcgca ggccggccat ccggctgcgc aggttctgcg cccagcgcag caccgcgagc
     2041 acgtccttca catgcgcggc ctcgagaaac ttcagcccgc cgaactttac gaacgggatg
     2101 ttgcggcgtg tcagctcgag ctcgagcgcc gcgctgtggt gcgagctgcg gaacagcgcg
     2161 acctgaccct tcagcgcgat gccgcgctcg cggtggcgca gcacctggtc ggcgacccag
     2221 gcggcctgcg ccgattcgtc gcgcaccgtg atcagcgacg ggcgctcgct cgaggccgca
     2281 tcgctccaca gctgcttgcc gaaacgctgc ggtgcgagcg cgatcaccgc gttcgacgcg
     2341 tcgaggatgg tctgc
//
LOCUS       DASRDG010000627         4466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_668807, whole genome shotgun sequence.
ACCESSION   DASRDG010000627 DASRDG010000000
VERSION     DASRDG010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668807"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1802
                     /locus_tag="VFA35_10525"
     CDS             <1..1802
                     /locus_tag="VFA35_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT56652.1"
                     /translation="AWLLQPGGAAAKPPGWLARVAERGYQWVLGRYARSLDWALASKG
                     LVMLILVAVVGLNVYLFAAAPKGFFPQQDSGQLNGGLRADQSISTKEMGRKLREVVDI
                     VHKDPAVDTVVGFTGGGRAGGGFMFVNLKPLGKGRTDTGNAVIARLRPQLARVTGLSV
                     FLGTVQDVRSGGRAASSTYQYTLKSDNLADLKKWAVKLTDEMKQSTVMTDVDSDQQEN
                     GVQTNVRVDRASASRLGVTSGAVDAALYNAFGQRQVATIYTELNQYHVVMEWAPLYTR
                     SPNSLADIYVPASRIVDSGGTAVPTEATAADTASSKTAPAVSANPGLKGASTGNVLAN
                     SVTRLVPLSALAKFVEDSAATSVNHQDGELATTVSFNLAEGQTLADARAAIAQAEANI
                     GMPTNVRGAFAGTAASAQESQGQQTLLIVAAIVVIYIVLGILYESLIHPVTVLSTLPS
                     AGVGAVLALLMFRMEFSIIALIGVFLLIGIVKKNAILIIDFALDAERSRGLTPLEAVR
                     EACLLRFRPILMTTLAAALGALPLAIGFGEGAELRQPLGVAIIGGLIASQLLTLLTTP
                     VVYMLLDKLRRRGTDERHLSRGIDPTLPSARAT"
     gene            1799..3220
                     /locus_tag="VFA35_10530"
     CDS             1799..3220
                     /locus_tag="VFA35_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZT56653.1"
                     /translation="MTNQNLCVNIGVIAIAAMLSACAVGPAYERPTSAAPAAFKEAPQ
                     GDAGWFPAAPADTLARGPWWELFGDAELNRLAAQVEVSNQNVAAAVAAYAQARALVAQ
                     QRASLFPLITADGSARRSGGNGAAAGTSNAFQASLGASWEPDVWGALRQGVTGAQASA
                     QASEAEFAGARLSAQGELATNYFSLRAADLELELLRRTVDGYERAATITRNRYDAGIA
                     PRTDVLQAQTQLANARADLASLTNTRAQLEHAIAVLIGKAPAEFTLAPAEWNMAVPAL
                     PLVVPSELLQRRPDIAAAERAAAAANANIGIQRSAYFPSIGLSASFGSAASRVGDLFN
                     ASTTLWSLGLSVAQTLFDAGATRARVAGAEAAHDGAVARYRQTVLTAFQAVEDQLSSA
                     RALAEQAGLRRAASEAADLTEQQLLNRYKAAQVSYTEVVTAQASALSARRTLAQLAAS
                     RQAAAIALVQALGGGWQQPAAAP"
     gene            3303..>4466
                     /locus_tag="VFA35_10535"
     CDS             3303..>4466
                     /locus_tag="VFA35_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZT56654.1"
                     /translation="MEKSWLRQYPAGVPANIDVEQYGSLVALLDESFKKYAGRMAYKF
                     MGKTISFAQVDEASRALAAYLQAQGLEKGDRVALMMPNVPQYPVACAAVLRAGYVVVN
                     VNPLYTPRELEHQLKDSGAKLIVIIENFATTLQQVLAAVPTKKIVVTALGDMLGFPKA
                     LIVNYVVRHVKKMVPAYELPGAVVFNEALRQGRSKPFKPAAVGPDDIAVLQYTGGTTG
                     VSKGAVLLHRNLVANILQSEAWYQPALKKIPAGQQIVTICALPIYHIFGFNANMMLAM
                     RMGGANILIANPRDLPSMFKELKGEAFHSFPAVNTLFQAMAHHPDFNTVDWSGLVISV
                     GGGMAVQSATAKLWLEKTGCPIVEGYGLSETSPSATCNPVDSTAYSGNIGLPMP"
BASE COUNT          687 a         1606 c         1554 g          619 t
ORIGIN      
        1 gcgcgtggct gctgcagccg ggcggcgcgg ccgcgaagcc gccggggtgg ctcgcgcgcg
       61 tggccgagcg cggctaccaa tgggtgctcg ggcgctacgc gcgcagcctc gattgggcgc
      121 tcgcgagcaa gggcctggtg atgctgatcc tggtcgcggt cgtcggcctg aacgtctacc
      181 tgttcgcggc cgcgccgaag ggcttcttcc cgcagcagga cagcgggcag ctgaacggcg
      241 gcctgcgcgc cgaccagagc atctcgacca aggagatggg ccgcaagctg cgcgaggtcg
      301 tcgacatcgt gcacaaggac ccggcggtcg acacggtcgt cggcttcacc ggcggcgggc
      361 gcgccggcgg cggcttcatg ttcgtgaacc tgaagccgct cggcaagggc cgcaccgaca
      421 ccggcaacgc ggtgatcgcg cggctgcgcc cgcagctcgc gcgcgtgacc ggcctgagcg
      481 tgtttctcgg caccgtgcag gacgtgcgct ccggcggccg cgccgccagc tcgacttatc
      541 agtacacgct gaagagcgac aacctggccg acctgaagaa gtgggccgtc aagctgaccg
      601 acgagatgaa gcagtcgacc gtgatgaccg atgtcgactc cgatcagcag gagaacggcg
      661 tgcagaccaa cgtccgggtc gaccgcgcca gcgcgagccg gctcggtgtc acctcgggcg
      721 cggtcgacgc ggcgctgtac aacgcgttcg gccagcgcca ggtcgcgacg atctacaccg
      781 agctgaacca gtaccacgtc gtgatggagt gggcgccgct gtacacgcgc agcccgaact
      841 cgctcgccga catctacgtg cccgcgagcc gcatcgtcga cagcggcggc acggcggtgc
      901 cgaccgaagc gacggccgcc gacacggcat cgtccaagac cgccccggcc gtctccgcga
      961 atccgggcct gaagggcgcc tcgaccggca acgtgctcgc gaacagcgtg acgcgcctgg
     1021 tgccgctctc ggcgctcgcg aagttcgtcg aggattcggc cgcgacctcg gtcaatcacc
     1081 aggacggcga gctcgccacc accgtctcgt tcaacctcgc cgaaggccag accctcgccg
     1141 acgcgcgcgc cgcgatcgcg caggccgagg ccaacatcgg catgccgacc aacgtgcgcg
     1201 gcgcgttcgc cggcaccgcc gcgagcgcgc aggagtcgca gggccagcag acgctgctga
     1261 tcgtcgcggc gatcgtcgtg atctacatcg tgctcggcat cctgtacgag agcctgatcc
     1321 acccggtcac ggtgctgtcg accctgccgt cggccggcgt cggcgcggtg ctcgcgctgc
     1381 tgatgttccg catggagttc tcgatcatcg cgctgatcgg cgtgttcctg ctgatcggca
     1441 tcgtcaagaa gaacgcgatc ctgatcatcg acttcgcgct cgacgccgag cgttcgcgcg
     1501 gcttgacgcc gctcgaagcg gtgcgcgagg cctgcctgct gcgcttccgc ccgatcctga
     1561 tgacgacgct cgccgccgcc ctgggcgcgc tgccgctggc gatcggcttc ggcgaaggcg
     1621 ccgagttgcg ccagccgctc ggcgtcgcga tcatcggcgg gctgatcgcg agccagctgc
     1681 tgacgctgct gaccacgccg gtcgtctaca tgttgctcga caagttgcgc cgccgcggca
     1741 ccgacgagcg ccacctgagc cgcggcatcg acccgaccct gccttccgcg agagccacat
     1801 gacgaaccag aatctctgcg tgaacatcgg cgtgatcgcg atcgccgcga tgctgtcggc
     1861 ctgcgcggtc gggcccgcct acgagcggcc gacttccgcg gcgcccgccg cgttcaagga
     1921 ggcgccgcag ggcgatgccg gctggttccc cgccgcgccg gccgacacgc tggcccgcgg
     1981 gccgtggtgg gagctgttcg gcgatgccga gctgaaccgg ctcgcggcgc aggtcgaggt
     2041 ctcgaaccag aacgtcgccg cggccgtcgc cgcgtatgcg caggcgcgcg cgctggtcgc
     2101 gcagcagcgc gcgtcgctgt tcccgctgat cacggccgac ggcagcgcgc ggcgcagcgg
     2161 cggcaacggc gccgccgccg gcacctcgaa cgcattccag gccagcctcg gtgcgagctg
     2221 ggagcccgat gtgtggggcg cactgcgcca aggcgtcacg ggtgcgcagg ccagcgcgca
     2281 ggcgagcgag gccgagttcg ccggcgcgcg cctgtcggcg cagggcgagc tcgcgaccaa
     2341 ctacttctcg ctgcgcgctg ccgacctcga gctcgagctg ctgcgccgca ccgtcgacgg
     2401 ctacgagcgc gccgcgacga tcacgcgcaa ccgctacgac gccggtatcg cgccacgcac
     2461 cgatgtgctg caggcgcaga cccagctcgc gaacgcgcgc gccgacctgg ctagcctgac
     2521 gaacacccgc gcgcagctcg aacacgcgat cgcggtgctg atcggcaaag cgccggccga
     2581 gttcacgctc gcaccggccg aatggaacat ggcggtgccc gcgctgccgc tggtggtgcc
     2641 gtcggaactg ctgcagcgcc ggcccgacat cgctgcggcc gagcgcgcgg ccgcggccgc
     2701 gaacgcgaac atcggcatcc agcgttcggc ctacttcccg agcatcggct tgagcgcgtc
     2761 gttcggcagc gcggcgagcc gcgtcggcga cctgttcaac gcgtcgacga cgctgtggtc
     2821 gctcggcctg tcggttgcgc agacgctgtt cgatgccggc gccacgcgcg cccgcgtcgc
     2881 cggcgccgag gccgcgcacg atggtgcggt ggcgcgctac cggcagaccg tgctgacggc
     2941 attccaggcg gtcgaggatc agctctcgtc cgcgcgcgcg ctggccgagc aggcgggcct
     3001 gcgccgcgcc gcgtcggaag cggccgacct gaccgagcag caactgctga accgctacaa
     3061 ggccgcacag gtcagctaca ccgaggtcgt gaccgcgcag gcctcggcgt tgagcgcgcg
     3121 ccgcacgctc gcgcagctgg cggcgagccg gcaggcggcg gcgatcgcgc tggtccaggc
     3181 gctcggcggc ggctggcagc agccggccgc ggcgccttag cgcccgatct gccccggggc
     3241 aagaccctag ccccgcgaac tccccgctcg gtcagaatgc cccgctgttc cgggagcaac
     3301 cgatggaaaa gagctggttg aggcagtacc ccgccggcgt gccggccaac atcgatgtcg
     3361 agcagtacgg ctcgctggtg gcgctgctgg acgagagctt caagaagtac gctggccgca
     3421 tggcgtacaa gttcatgggc aagaccatca gcttcgcgca ggtcgacgag gcctcgcgcg
     3481 cgcttgccgc ctacctgcag gcgcaggggc tcgagaaggg cgaccgcgtc gcgctgatga
     3541 tgcccaacgt gccgcagtac ccggtcgcgt gcgcggcggt gctgcgcgcg ggctatgtcg
     3601 tcgtcaacgt caacccgctg tacacgccgc gcgaactcga gcaccagttg aaggactcgg
     3661 gcgccaagct gatcgtcatc atcgagaact tcgcgaccac gctgcagcag gtgctggccg
     3721 cggtgccgac caagaagatc gtcgtcaccg cgctcggcga catgctcggc ttcccgaagg
     3781 ccttgatcgt caactacgtc gtgcgccacg tgaagaagat ggtgccggcc tacgagctgc
     3841 cgggcgccgt cgtgttcaac gaggcgctcc ggcagggccg cagcaagccg ttcaaaccgg
     3901 ccgcggtcgg ccccgacgac atcgcggtgc tgcagtacac cggcggcacc accggcgtca
     3961 gcaagggcgc cgtgctgctg caccgcaact tggtcgcgaa catcctgcag tccgaggcct
     4021 ggtaccagcc ggcgctgaag aagatccccg ccggccagca gatcgtgacg atctgcgcgc
     4081 tgccgatcta tcacatcttc ggcttcaacg cgaacatgat gctggcgatg cgcatgggcg
     4141 gcgccaacat cctgatcgcc aacccgcgcg acctgccgag catgttcaag gagctgaagg
     4201 gcgaggcctt ccacagcttc ccggcggtca acacgctgtt ccaggcgatg gcgcaccacc
     4261 ccgacttcaa caccgtcgac tggagcggcc tggtgatctc ggtcggcggc ggcatggcgg
     4321 tgcagagcgc caccgccaag ctctggctcg agaagaccgg ctgcccgatc gtcgagggct
     4381 acggcctgtc ggagacgtcg ccgtcggcca cctgcaaccc ggtcgacagc accgcctaca
     4441 gcggcaacat cggcctgccg atgccg
//
LOCUS       DASRDG010000628         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_328387, whole genome shotgun sequence.
ACCESSION   DASRDG010000628 DASRDG010000000
VERSION     DASRDG010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328387"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFA35_10540"
     CDS             <1..518
                     /locus_tag="VFA35_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HZT56655.1"
                     /translation="GYKGYAEYDTVAAVKAALRIPVCANGDIDSPEKARDVLAATGAD
                     AIMIGRAAQGRPWIFREIAHFLDTGEHLPAPEVAQAKAWLLEHLHDHYGLYGWDDDGT
                     GVRSARKHIGWAVRALPGGEAFRARMNLLGSCEAQVRAVTDWFDELADTHHRLPRTAV
                     DEQGTQEEAFA"
     gene            515..754
                     /locus_tag="VFA35_10545"
     CDS             515..754
                     /locus_tag="VFA35_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZT56656.1"
                     /translation="MKKPTHIEECIRDSLEIYFKDLRGVEPAAMYEMILGVVEKPLLD
                     VVMKHADGNQSRAAEWLGINRNTLRRKLLDHKLIK"
     gene            751..>2209
                     /gene="purH"
                     /locus_tag="VFA35_10550"
     CDS             751..>2209
                     /gene="purH"
                     /locus_tag="VFA35_10550"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZT56657.1"
                     /translation="MKNPLTALLSVSDKTGVLEFAQALHALGVKLLSTGGTAKLLADA
                     GLPVTEVAEHTGFPEMLDGRVKTLHPTIHGGLLARRDRPAHMAAIAQHGIATIDLLVV
                     NLYPFEATVAKPGCTLDDAIENIDIGGPAMVRSAAKNWKDVAVLTDAAQYPQVLAELQ
                     ADGAVSASTRFALAVAAFNRISNYDAAISDYLSSLQPDGSRAAFPAQSNARFVKLQDL
                     RYGENPHQSAAFYRDLYPAPGSLVTATQLQGKALSYNNIADADAAWECVKSFGTDGPA
                     CVIVKHANPCGVALGADAAQAYAKAFKTDPTSAFGGIIAFNCELDGDGAALVAKQFVE
                     VLMAPSFTPEALAFFAAKPNTRVLQIALPAGGPSDWEQGRNAQDVKRVGSGLLIQTAD
                     NREITAAELKVVTDRQPTPAQLADLLFAWKVAKYVKSNAIVFCGGGMTLGVGAGQMSR
                     VDSARIASIKAANAGLSLQGSAVASDAFFPFRDGID"
BASE COUNT          387 a          779 c          737 g          306 t
ORIGIN      
        1 aaggctacaa gggctacgcc gaatacgaca cagtggctgc cgtgaaggcc gcgctgcgta
       61 ttccggtctg cgcgaacggc gacatcgatt cgcccgagaa ggcgcgcgac gtgctcgcag
      121 ccaccggcgc cgacgcgatc atgatcggcc gcgccgcgca gggccggccg tggatcttcc
      181 gcgagatcgc gcactttctc gacaccggcg agcatctgcc ggcgcccgag gtcgcgcagg
      241 ccaaggcctg gctgctcgag cacctgcacg accactacgg cctgtacggc tgggacgacg
      301 acggcaccgg cgtgcgcagc gcgcgcaagc acatcggctg ggcggtgcgc gccctgcccg
      361 gcggcgaggc gttccgcgcc cgcatgaacc tgctcggcag ttgcgaggcg caggtccgcg
      421 ccgtcaccga ctggttcgac gaactcgccg acacgcacca ccggttgccg cgcaccgccg
      481 tcgacgaaca agggacacaa gaagaagcat tcgcatgaag aaaccgaccc acatcgagga
      541 gtgcatccgt gacagcctcg agatctactt caaggacttg cgcggcgtgg aacccgcggc
      601 gatgtacgag atgattctcg gcgtggtcga gaagccgttg ctcgacgtcg tgatgaagca
      661 cgccgacggc aaccagagcc gcgcggccga gtggctgggc atcaaccgca acacgctgcg
      721 ccgcaagctg ctcgaccaca aactcatcaa atgaagaacc cactcaccgc cctgctctcg
      781 gtctccgaca agaccggcgt cctcgaattc gcgcaggcgc tgcatgcgct cggcgtcaag
      841 ctgctctcga ccggcggcac cgccaagctg ctcgccgatg ccggcctgcc ggtcacggag
      901 gtggccgagc acaccggctt tcccgagatg ctcgacggcc gcgtcaagac gctgcacccg
      961 acgatccacg gcggcctgct ggcgcgccgc gatcggcccg cgcacatggc ggcgatcgcg
     1021 cagcacggca tcgcgacgat cgatctgctg gtcgtgaacc tctacccgtt cgaggcgacc
     1081 gtcgcgaagc ccggctgcac cctcgacgac gcgatcgaga acatcgacat cggcggcccg
     1141 gcgatggtgc gctcggcggc gaagaactgg aaggacgtgg cggtgctgac cgatgcggcg
     1201 cagtacccgc aggtgctggc cgaattgcag gccgacggcg ccgtgagcgc atcgaccagg
     1261 ttcgcgttgg ccgtcgccgc gttcaaccgc atctcgaact acgacgcggc gatcagcgac
     1321 tacctctcgt cgctgcagcc cgacggctcg cgcgcggcct tcccggcgca gtcgaacgcg
     1381 cgcttcgtca agctgcagga cctgcgctac ggcgagaacc cgcaccagag cgcggcgttc
     1441 taccgcgacc tgtaccccgc acccggctcg ctcgtcaccg caacgcagct gcagggcaag
     1501 gcgctctcgt acaacaacat cgccgacgcc gacgcggcct gggagtgcgt gaagtcgttc
     1561 ggcaccgacg gcccggcctg cgtgatcgtc aagcacgcca acccctgcgg cgtcgcgctc
     1621 ggcgcggacg cggcgcaggc ctacgccaag gccttcaaga ccgacccgac ctcggccttc
     1681 ggcggcatca tcgcgttcaa ctgcgaactc gacggcgacg gcgccgcgct ggtcgcgaag
     1741 cagttcgtcg aggtgctgat ggcgccgagc ttcacgcccg aggcgctggc gttcttcgcg
     1801 gcgaagccga acacgcgggt gttgcagatt gcattgcccg cgggcggacc gtccgattgg
     1861 gagcaaggcc gcaacgcgca ggacgtgaag cgcgtcggct ccgggttgct gatccagacc
     1921 gccgacaacc gcgagatcac cgcggccgag ctgaaggtcg tgaccgacag gcagccgaca
     1981 cccgcacaac tcgccgacct gctgttcgcc tggaaggtcg cgaagtacgt gaagagcaat
     2041 gcgatcgtgt tctgcggggg cggcatgacg ctgggcgtcg gggccgggca gatgagccgt
     2101 gtcgactcgg cgcgcatcgc aagcatcaag gccgccaacg ccgggctctc gctgcagggc
     2161 tcggccgtcg cgagcgacgc gtttttcccg tttcgcgacg gcatcgatg
//
LOCUS       DASRDG010000629         5404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_98501, whole genome shotgun sequence.
ACCESSION   DASRDG010000629 DASRDG010000000
VERSION     DASRDG010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98501"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFA35_10555"
     CDS             complement(<1..488)
                     /locus_tag="VFA35_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein"
                     /protein_id="HZT56658.1"
                     /translation="MIRSFDAHELDELVTELVRPGAIAELAILAGCLVAAWIIVRLLR
                     GSERPVASVWFGNKIFDGVLFPVIALALAYAARVVLAGTVKPAVFKVAIPILISLVVI
                     RLSVRVLSRTFPGVRWVRTVERSISWLAWIAVVLWVTGVSPVILDAMDEVHWKVGATD
                     VT"
     gene            complement(565..1497)
                     /locus_tag="VFA35_10560"
     CDS             complement(565..1497)
                     /locus_tag="VFA35_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HZT56659.1"
                     /translation="MTTAFYSHPDCRRHDMGRGHPECPQRLDAIDDYLLATGLDVALE
                     RHEAPLVDLRDVAHAHSSGYVAELRDALAQLPADGSLLALDPDTCASAGTWAAVQRAA
                     GAAVAATDAVIDGRAANAFCSVRPPGHHATRNAAMGFCFFNNVAIAARHALDVRGLQR
                     VAIIDFDVHHGNGTEDIIAGDERVLMVSIFQDKLYPFSGGVPMGDNMVNVPVPAYTRG
                     AAVRQLIEDRWMARLDAFAPEMIFVSAGFDAHRDDELGQLGLVEADYTWITEKIKALA
                     DKHAGGRIVSCLEGGYNLGALARSVAAHLRVLMA"
     gene            1535..2476
                     /locus_tag="VFA35_10565"
     CDS             1535..2476
                     /locus_tag="VFA35_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZT56660.1"
                     /translation="MDKRVDNTPLAAQLIELVDQIATIIVGKRTQIEDCVACMLAGGH
                     LLIEDVPGVGKTTLAHALAVSLGLRFSRTQFTADLMPSDLIGVSVYERTKESFVFHPG
                     PVFAQVLLADEINRAGPKTQSALLEAMEEHQVTVEGETRALPEPFFVIATQNPSDQLG
                     TYPLPESQLDRFLMCLTLGYPDQASERALLAGQDRREAIHGLRAVMTPPQLASAQQAV
                     QAVHVSDALLDYLQALIAATRSGRWFVEGLSPRAGIGVLRAARARALLGQRDYVAPDD
                     VQYVLPQTIAHRLVPAAGAGRGRVEQVRAMIEAVALP"
     gene            2484..3488
                     /locus_tag="VFA35_10570"
     CDS             2484..3488
                     /locus_tag="VFA35_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZT56661.1"
                     /translation="MAELAAPPSAGLNPAAHARARFRAWVDARQPRTDTLLLTQRNVY
                     ILPTRAGWMFGLTLIVLLLASINYQLNLGYVLTFLLAGSGVVSMHLTHATLCGLTLHL
                     KPVTPVFAGDPAVLDIVLTSPGAARFGIGLKVAGAPEATLTWIDVPALGQAHAQVSFV
                     PPTRGLHATPTLGAETRFPLGLFRVWTVWRPAAQLLVYPPPERPPAPLPAARALPGGP
                     TQSRSADGGEVEGVRAYRRGDPLKLIAWKKAAKTLDSGGDLVSRDTSASAHQELWLAW
                     QACGALAPEERLARLTAWTLAAHRAGADYGLRLPGVEIAPADGDAQRRRCLEALALWR
                     "
     gene            3479..>5404
                     /locus_tag="VFA35_10575"
     CDS             3479..>5404
                     /locus_tag="VFA35_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HZT56662.1"
                     /translation="MALTGRLARGPRIARLWPGWSRLPRDARDTLFLLFVIGWTVLPH
                     ATHLPLWCSLLTLVVLLWRAQLALSNAPLPGRWTLVAVLIVCAALTFWSYRTLLGKEP
                     GVTLAVVLMALKTLELRARRDAFVVFFLGFFLILTHFLYSQSLAVAGAMLVSVWGLLT
                     ALVLAHMPVGQPSLRQAARLAARTALFGAPIMVLLFVLFPRIAPLWGVPQDGLSKTGL
                     SSTMRMGSVAEIAIDDSIALRVRFAGLPPPAQAMYFRGPVLTRFDGVEWRALPTRSFD
                     ALGSLPPNLRTSGAPVRYEMTLEPLRLTSLPLLEATATAPTIEGYRVAGMPDLQWRTD
                     KPLFERVRFSAEAFTSFRYGPVDRVADPEFDLQDALALPPGYNPRTLAWAAALRREPR
                     YAAAEPRVLAQALLQHIRSGGFSYTLAPGSYGDERGHDAIDEFWLDRRAGFCEHFAAA
                     FVVVMRAAGVPARVVTGYQGVDPVPSDGYYTVRQSAAHAWTEYWQPGVGWVRADPTAA
                     VAPERISRARRLRPAPGLVAGTIGNVSPELLAQLRDGWEALNNRWNQWVLNYSRGQQL
                     DLLKDLGFRAPSWEDLALLLIGAASALALAGAGWAWWDRHRVDPWTRQMDRLRRALQR
                     AGLSTGAHEPPRALAQRV"
BASE COUNT          732 a         1942 c         1969 g          761 t
ORIGIN      
        1 aaggtcacgt ccgtcgcgcc gaccttccag tgcacttcgt ccatcgcgtc gaggatgacc
       61 ggcgagacgc cggtgaccca gaggacgacc gcgatccagg ccagccacga gatgctgcgc
      121 tcgacggtgc ggacccagcg cacgcccgga aaggtgcggc tcaggacgcg cacgctgagg
      181 cggatgacga cgagcgagat caggatcggg atcgcgacct tgaacaccgc cggcttgacg
      241 gtccccgcga gcacgacgcg cgccgcatac gccagggcga gcgcgatgac cgggaagagg
      301 acgccgtcga agatcttgtt gccgaaccag accgaggcga ccggccgctc gctgccgcgc
      361 agcaggcgga cgatgatcca ggcggcgacc aggcagccgg cgaggatcgc cagctcggcg
      421 atggcgcccg gacgaaccag ctcggtaacg agctcgtcga gctcgtgggc gtcgaaggaa
      481 cggatcacgg ccgggcgccg ggccgcccca agccgggccg cgcccccccg gggggcagcg
      541 aacgaagaga gcgtgggggc accgtcacgc catcaggacg cgcaggtgcg cggcgacgct
      601 gcgcgcgagc gcgccgaggt tgtagccgcc ttctaggcac gagacgatgc ggccgcccgc
      661 atgcttgtcg gcgagcgcct tgatcttctc ggtgatccag gtgtagtcgg cctcgaccag
      721 gccgagctgg ccgagctcgt cgtcgcggtg ggcgtcgaag ccggcggaga cgaagatcat
      781 ctcgggcgcg aaggcgtcga gccgcgccat ccagcggtcc tcgatgagct gccgcaccgc
      841 ggcgccgcgc gtgtaggccg gcacgggcac gttgaccatg ttgtcgccca tcggcacgcc
      901 gccgctgaac gggtagagct tgtcctggaa gatgctaacc atcagcacgc gctcgtcgcc
      961 ggcgatgatg tcttcggtgc cgttgccgtg gtgcacgtcg aagtcgatga tcgccacgcg
     1021 ctgcagcccg cgcacgtcga gcgcgtggcg cgcggcgatc gcgacgttgt tgaagaagca
     1081 aaagcccatg gctgcgttgc gcgtcgcgtg gtggccgggc gggcgcaccg agcagaaggc
     1141 gttggcggcg cggccgtcga tcaccgcatc ggtggcagcc accgcggcgc cggcggcgcg
     1201 ctgcaccgcg gcccaggtgc cggcgctggc gcaagtatcg gggtcgagcg cgagcagcga
     1261 gccgtcggcc ggcagctgcg cgagcgcatc gcgcagctcg gcgacatagc cgctgctgtg
     1321 cgcgtgcgcc acgtcgcgca ggtcgaccag cggcgcttca tggcgctcga gcgccacatc
     1381 gagcccggtc gcgagcaggt agtcgtcgat cgcgtcgagg cgctgcgggc actcggggtg
     1441 gccgcggccc atgtcgtggc ggcggcaatc gggatggctg tagaaggcgg tcgtcatcgg
     1501 cttacggttt cttacttttc gggtaaggtg gggcatggac aaacgcgtcg acaacacgcc
     1561 gctggccgcg cagctgatcg agctcgtcga tcagatcgcg acgattatcg tcggcaagcg
     1621 cacccagatc gaagactgcg tcgcctgcat gctcgccggc ggtcacctgc tgatcgaaga
     1681 cgtgcccggc gtcggcaaga cgacgctggc gcacgcactc gcggtctcgc tgggcctgcg
     1741 cttctcgcgc acccagttca cggccgacct gatgccgtcc gacctgatcg gcgtcagcgt
     1801 ctacgagcgc acgaaggaga gcttcgtgtt ccaccccggc ccggtgttcg cgcaggtgct
     1861 gctggccgac gagatcaacc gcgccgggcc gaagacgcag agcgcgctgc tcgaagcgat
     1921 ggaggagcat caggtgacgg tcgagggcga gacccgcgcg ctgcccgagc ccttcttcgt
     1981 gatcgcgacg cagaacccga gcgaccaact cggcacctac ccgttgcccg aatcgcagct
     2041 cgaccgcttc ctgatgtgcc tgaccctggg ctaccccgac caggcgagcg agcgcgcgct
     2101 gctcgccggg caagaccggc gcgaagcgat ccacggcctg cgcgcggtga tgacgccgcc
     2161 gcaactggcc tccgcgcagc aagcggtgca ggcggtgcat gtgtcggatg cgctgctcga
     2221 ctacctgcag gcgctgatcg cggcaacgcg ctcgggccgc tggttcgtcg agggcctgag
     2281 cccgcgcgcc ggcatcggtg tgctgcgcgc cgcgcgcgcc cgcgcgctgc tcggccagcg
     2341 cgactacgtg gcgcccgacg acgtgcagta cgtgctgccg cagaccatcg cacaccgcct
     2401 cgtgccggcc gccggcgccg ggcgcgggcg cgtcgagcag gtgcgggcca tgatcgaggc
     2461 ggtggcgctg ccgtaggcat cgcatggccg aactcgccgc gccgcccagc gccgggctga
     2521 accccgccgc ccacgcccgc gcgcgctttc gcgcctgggt cgacgcgcgc cagccgcgca
     2581 ccgacacgct gctgctgacc cagcgcaacg tctacatcct gccgacgcgc gccggctgga
     2641 tgttcgggct gacgttgatc gtgctgctgc tcgcgtcgat caactaccag ctcaacctcg
     2701 gctacgtgct gacctttctg cttgccggca gcggcgtcgt ctcgatgcac ctgacgcacg
     2761 cgacgctgtg cggcctgacg ctgcacctga agccggtgac gccggtgttc gcgggcgacc
     2821 cggccgtgct cgacatcgta ctgaccagcc ccggcgccgc gcgcttcggc atcgggctga
     2881 aggtcgcggg cgcacccgag gccacgctga cctggatcga cgtgccggcg ctcggccagg
     2941 cgcatgcgca ggtcagcttc gtgccgccaa cgcgcggcct gcacgcgacg ccgacgctcg
     3001 gcgcggagac acgcttcccg ctcgggctgt tccgcgtctg gaccgtctgg cgcccggccg
     3061 cgcagctgct cgtctacccg ccacccgagc ggccgcccgc gccgctgccg gcggcacggg
     3121 cgctgccggg cgggccgacg cagtcgcgca gtgcggacgg cggcgaggtc gaaggcgtgc
     3181 gcgcataccg gcgcggcgac ccgctgaagc tgatcgcctg gaagaaggcc gcgaaaacgc
     3241 tggacagcgg cggcgacctc gtcagccgcg acacgagcgc gtcggcgcac caggagttgt
     3301 ggctggcctg gcaagcctgc ggcgcgctcg cgcccgagga gcgcctggca cgcctgaccg
     3361 cgtggacgct cgccgcacac cgggcaggcg ccgactacgg cctgcgcctg cccggcgtcg
     3421 agatcgcccc ggccgacggc gacgcgcaac gccgccgctg tctggaggcg ctggcactgt
     3481 ggcgctgacc ggccggctcg cgcgcggccc gcgcatcgct cgcctctggc cgggctggag
     3541 ccgcctgccg cgcgatgcgc gcgacacgct gttcctgctg ttcgtgatcg gctggacggt
     3601 gctgccgcat gccacgcact tgccgctgtg gtgctcgctg ctgacgctgg tggtgctgct
     3661 gtggcgcgcg cagctggcgc tctcgaacgc gccgctgccc gggcgctgga cgctcgtggc
     3721 ggtgctgatc gtctgcgccg cgctgacctt ctggtcgtac cgcacgctgc tcggcaagga
     3781 gccgggcgtg acgctcgcgg tggtgctgat ggcgctgaag acgctcgagt tgcgggcgcg
     3841 gcgcgacgcc ttcgtcgtgt tctttctcgg cttcttcctg atcctcacgc acttcctcta
     3901 ctcacaatcg ctggccgtcg ccggcgcgat gctggtgtcg gtctggggcc tgctgaccgc
     3961 gctggtgctc gcgcacatgc cggtgggcca gccgagcctg cgccaggcgg cgcggctcgc
     4021 ggcgcgcacc gcgctgttcg gcgcgccgat catggtgctg ctgttcgtgc tgttcccgcg
     4081 catcgcgccg ctgtggggtg tgccgcagga cgggctgtcg aagaccggcc tgtcgagcac
     4141 gatgcgcatg ggctcggtcg ccgagatcgc aatcgacgac agcatcgcgc tgcgcgtgcg
     4201 cttcgcgggc ctgccgccgc cggcgcaggc catgtacttc cgcgggccgg tgctgacacg
     4261 cttcgacggc gtcgagtggc gggcgctgcc gacgcgatcg ttcgacgcgc tcgggtcgct
     4321 gccgcccaac ctgcgcacca gcggcgcccc tgtgcgctac gagatgacgc tcgagccgct
     4381 gcgcctgacc agcctgccgc tgctcgaagc gaccgccacg gcgccgacga tcgagggcta
     4441 ccgcgtcgcc ggcatgcccg atctgcaatg gcgcaccgac aagccattgt tcgagcgggt
     4501 tcgtttcagc gccgaggcgt tcacgagctt tcgctacggg ccggtcgacc gcgtggccga
     4561 ccccgaattc gacctgcagg acgcgctcgc gttgccaccc ggctacaacc cgcgcacgct
     4621 cgcgtgggcc gcggcgctgc gccgcgagcc gcgctacgcc gccgccgagc cgcgcgtgct
     4681 cgcgcaggcg ctgctgcagc acatccgcag cggcggcttc agctacacgc tcgcaccggg
     4741 cagctacggt gacgagcgcg gccacgacgc gatcgacgag ttctggctcg accgccgcgc
     4801 gggcttttgc gagcacttcg cggccgcatt cgtcgtcgtg atgcgcgcgg cgggcgtgcc
     4861 ggcgcgggtc gtcaccggct accagggcgt cgacccggtg ccgagcgacg gctactacac
     4921 ggtgcgccag agcgcggcgc acgcctggac cgaatactgg cagccgggcg tcggctgggt
     4981 gcgcgccgac ccgaccgcgg cggtcgcacc cgagcgcatc agccgcgcgc ggcgcctgcg
     5041 gcccgcaccg ggcctggtgg caggcacgat tggcaacgtc agcccggagc tgctcgcaca
     5101 gctgcgcgac ggctgggagg cgctgaacaa ccgctggaac cagtgggtgc tgaactactc
     5161 gcgcggccag cagctcgacc tgctgaaaga tctgggcttt cgcgcgccga gctgggaaga
     5221 cctcgcgctg ctgctgatcg gggccgcgag cgcgctggcg ctcgccggcg ccggctgggc
     5281 ctggtgggac cgccatcgcg tcgacccctg gacgcgccag atggatcgcc tgcgcagagc
     5341 gctgcaacgc gccggcctga gcactggggc gcacgagccg ccgcgcgcgc tggcgcagcg
     5401 cgtg
//
LOCUS       DASRDG010000630         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_328937, whole genome shotgun sequence.
ACCESSION   DASRDG010000630 DASRDG010000000
VERSION     DASRDG010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328937"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFA35_10580"
     CDS             <1..888
                     /locus_tag="VFA35_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56663.1"
                     /translation="PPEPHWGASILPADRPELRAALRFDRFTQFSKTGQQFNEIEETA
                     GFNLLSVSYTENTPADPDLFFTVAGGVGYSGDQPTRFFQNDYVHMILGQPGVPVGATR
                     DGVEYTASGSLTKWFDGPRLFDTGDPFDDAAWRTRFFLGAGASTSTIYHEAFAHGGAT
                     LFVPDLVLSHGFRFTLADRITFPTGEDEFHDLASVSDVAQASVGLVPAHVHADSPVLE
                     FLGNPEFGVSITYDSGFFQRAGEPISIWFAGFYVQWPTGLRFETWNDFANGTDFGPTY
                     GFLVSVDLFSLFHNTRDRW"
     gene            891..1511
                     /locus_tag="VFA35_10585"
     CDS             891..1511
                     /locus_tag="VFA35_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56664.1"
                     /translation="MRRPAFLLPLVFALLACALTSCTTGVLIANADTGYEAKGDRYIR
                     LRLETVRLDVVDLQPGQATMPGALCCKDHTDASQRALRFRLPFQAWNGSGEPWPIRLR
                     MFQLTRTGTGEPAGAPVVLEAPDDDMVVALVPPGSGTTFDVLVHVAGLPACDDPLQAE
                     YRLTVQDENGEVLLVKTMSAGSYSQLAQVARFVGLATLLLVVAAAI"
     gene            1579..1896
                     /locus_tag="VFA35_10590"
     CDS             1579..1896
                     /locus_tag="VFA35_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZT56665.1"
                     /translation="MNRVLLRASLALVPGALCAPLLAQGEPKFAPPVRLMAGGKFLGG
                     NRLFPSPVLRDMNGDGRLDVVVGDLFGRLTVALQQPGDALSFGAETELDGADGKRLDF
                     HNW"
     gene            1921..>2380
                     /locus_tag="VFA35_10595"
     CDS             1921..>2380
                     /locus_tag="VFA35_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZT56666.1"
                     /translation="MDFDGDGVIDIVAGTFDGSPHLARGSGKGWLQPEQILDRDGARI
                     VFNQFWNFDSKKWDSTRRCDPEGEQDRREGHLTSAVAFDVDGDGALDLVLGDHSTGRI
                     CWRRNEGTNQKPAFATRNQPLLAGGAPVDVPGTVATLRVVDWNGDGRPDLV"
BASE COUNT          341 a          865 c          822 g          352 t
ORIGIN      
        1 ccgcccgagc cgcactgggg cgcgtccatc ctgccggcgg atcggcccga gctgcgcgcc
       61 gcgctgcgct tcgatcgctt cacgcagttc agcaagaccg gtcagcagtt caacgagatc
      121 gaagagacgg cgggcttcaa cctgctgtcg gtgagctaca cggagaacac gcccgcggat
      181 ccggacctgt tcttcaccgt ggccggcggc gtgggctaca gcggcgacca gccgacgcgc
      241 ttcttccaga acgactacgt gcacatgatc ctcggccagc ccggcgtgcc ggtgggcgcg
      301 acgcgcgacg gtgtcgagta cacggcgtcg ggcagcctga cgaagtggtt cgacggcccg
      361 cggctgttcg acaccggcga cccgttcgac gatgccgcct ggcgcacgcg cttcttcctc
      421 ggcgccggcg cgtcgaccag cacgatctac cacgaagcgt tcgcccacgg cggcgcgacg
      481 ctgttcgtgc ccgacctggt cctgagccac ggcttccggt tcacgctcgc cgaccgcatc
      541 accttcccga ccggcgaaga cgaattccac gacctcgcca gcgtgagcga cgtcgcgcag
      601 gcgtcggtcg gactggtgcc cgcgcacgtg cacgccgact cgccggtgct cgagtttctg
      661 ggcaacccgg agttcggcgt gtcgatcacc tacgacagcg gtttcttcca gcgcgccggc
      721 gagccgatct cgatctggtt cgcgggcttc tacgtgcagt ggccgacggg gctgcggttc
      781 gagacctgga acgacttcgc caacggcacg gacttcgggc cgacctacgg cttcctggtc
      841 agcgtcgatc tgttctcgct gttccacaac acccgcgacc ggtggtgacc atgcgcagac
      901 ccgccttcct gttgccactg gtgttcgcgc tgctcgcctg cgcgctgacg tcgtgcacga
      961 ccggcgtgct gatcgcgaac gccgacaccg gctacgaggc gaagggcgac cgctacatcc
     1021 ggctccggct cgagaccgtg cggctcgacg tcgtcgacct gcagcccggg caggcgacga
     1081 tgcccggcgc gctgtgctgc aaggaccaca cggacgcgtc gcagcgcgcg ctgcggttcc
     1141 ggctgccgtt ccaggcctgg aacggcagcg gcgagccgtg gccgatccgg ctccgcatgt
     1201 tccagctcac gcgcaccggc accggcgagc ccgcgggcgc gcccgtggtg ctggaggcgc
     1261 ccgacgacga catggtcgtc gcactggtcc cgccgggcag cggcacgacg ttcgacgtgc
     1321 tcgtgcacgt ggcggggctg ccggcgtgcg acgacccgct gcaggccgag taccggctca
     1381 cggtgcagga cgagaacggc gaggtgctgc tggtgaagac gatgtcggcc ggcagctaca
     1441 gccagctcgc gcaggtggcg cgcttcgtcg gcctggcgac gctgctgctg gtcgtcgccg
     1501 cggcgatctg acgcaggccg accgccgcct ctgctacgct accggcgcgg acgcgagccg
     1561 cgcccgcgag gagaccccat gaaccgagtc ctgctccgcg ccagcctggc gctcgtcccc
     1621 ggcgcgctct gcgcaccgct gctcgcgcag ggcgagccga agttcgcgcc gccggtgcgc
     1681 ctgatggctg gcggcaagtt tctcggcggc aaccgcctgt tcccgtcgcc ggtgctccgc
     1741 gacatgaacg gcgacggccg cctcgacgtc gtcgtcggcg atctcttcgg ccgcctcacc
     1801 gtggcgctgc agcagcccgg cgacgcgctc tcgttcggcg ccgagaccga gctggacggc
     1861 gcggacggca agcggctcga cttccacaac tggtgatgca tcggcatgag tccacagttc
     1921 gtggacttcg acggggacgg cgtgatcgac atcgttgccg gcaccttcga cggctcgccg
     1981 cacctcgcgc gcggcagcgg caagggctgg ctgcagcccg aacagatcct cgaccgcgac
     2041 ggcgcgcgca tcgtgttcaa ccagttctgg aacttcgaca gcaagaaatg ggactcgacc
     2101 aggcgctgcg accccgaggg tgagcaggac cgtcgcgagg gacacctcac gtccgcggtc
     2161 gcgttcgacg tcgacggcga cggcgcgctc gacctggtgc tcggcgacca ctcgaccggc
     2221 cgaatctgct ggcgccgcaa cgaaggcacg aaccagaagc cggcgttcgc gaccaggaac
     2281 cagccgctgc tggccggcgg cgcgccggtc gacgtgccgg gcacggtcgc gacgctgcgc
     2341 gtcgtcgact ggaacggcga cggccgcccc gacctcgtcg
//
LOCUS       DASRDG010000631         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_211857, whole genome shotgun sequence.
ACCESSION   DASRDG010000631 DASRDG010000000
VERSION     DASRDG010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211857"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1003
                     /locus_tag="VFA35_10600"
     CDS             143..1003
                     /locus_tag="VFA35_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZT56667.1"
                     /translation="MTDTLVLGAVAYDAKVITIWDGFQRWFAAQGLPFAYVLYSDYER
                     QVEQHFAGAVHVTWNSPLAWLQAQRVAAARGRKAQAIAMRDTDRDLTSVIVVRSDSPI
                     RSVADLRGRTVGTGAADSPQATLIPLQHLAEAGVEPEKACTVRRFDVLVGKHGDHVGG
                     ERDAARALLQGEVDAACMIDGNHLAFVNEGTLPAGRTRVLAQTAPYDHCNFTALDGAP
                     DGAPAALIARFRELLLAMRYDDPQVRPLMDLEGLKAWREGRTSGYAALERACRRTDWL
                     QPFLRRMGAA"
     gene            1000..2190
                     /locus_tag="VFA35_10605"
     CDS             1000..2190
                     /locus_tag="VFA35_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZT56668.1"
                     /translation="MTGTRVLDLGQLGFDRGAHILVQRELLRLARGEALAVRGAADGL
                     AAHLRAWARAMGHEFEAAPGLAGVVRAGPAAAARWRGAERAGSAAADGAVAAAPLHWG
                     LAARGALVEAGMPAFAFALAQKDEVWADDPGALYRQAAAAQWDPETAIPWAAAAASSG
                     DGEDALVQVLTYLIENETAAALVPARFCAQTHPHYREVLQLLAIQAADEARHIEVFTR
                     RAALRRTELGTSTVGGQASLKTLVDEPDFALASFLLSVLGEGSFLHLLGFLHVHAPDP
                     CTAEIMRLAAQDESRHVAFGMAHLQRHGARDATLRSRLALAIERRHDALRHTAGLDQN
                     VFDALVVLAARGFAPEAIAGGHAAVAELVQQMDAGRRARLRKLGFEGAEAAALSALHT
                     RNFM"
     gene            complement(2183..2575)
                     /locus_tag="VFA35_10610"
     CDS             complement(2183..2575)
                     /locus_tag="VFA35_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZT56669.1"
                     /translation="MGLYLDALRAHGLTQGEAHLLAHLAQVRAATVGELHEVLAHKRS
                     TLTSILDRLVERGLVTRDVGEADRRTFVIELTAPGRRVAARTRDHVLDLERAVGRQFT
                     AADLATFRAVLAAIERTADQRTAARRVT"
     gene            complement(2577..>3112)
                     /locus_tag="VFA35_10615"
     CDS             complement(2577..>3112)
                     /locus_tag="VFA35_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56670.1"
                     /translation="LRATGTALAAGRRRRAGAREAAMNDAIALDTAFARAFERGEVGN
                     DSFRHREHLRLAWACLAEGPSTEAACARVGAAIRAFARRAGSAQRYHETLTQFWVRLL
                     AGVQAGVRAAACDAIGLDAVLAAHPWLLDKDLPLAYYTRALLFGDAARAAWRPPDLRP
                     LPSHAPAADPRAPPRHA"
BASE COUNT          385 a         1137 c         1200 g          390 t
ORIGIN      
        1 ggcgtcgagc ggcggttccg cgacgcgcgc gcggcgatga tcatggcgcc caccagcgac
       61 cagctgttcg acttcatcgg caaggccgtc tgcggcctgc ccctgttctg agccgcggcc
      121 cccggacatg gaggaggcga agatgaccga cacgctcgtc ctcggcgccg tcgcctacga
      181 cgccaaggtg atcacgatct gggacggctt ccagcgctgg ttcgccgcgc agggcctgcc
      241 gttcgcgtac gtgctctatt ccgactacga acggcaggtc gaacagcatt tcgccggcgc
      301 ggtgcacgtg acgtggaact cgccgctcgc gtggctgcag gcgcagcgcg tggccgcggc
      361 gcgcggccgc aaggcgcagg cgatcgcgat gcgcgacacc gaccgcgacc tcacgtcggt
      421 gatcgtcgtg cgcagcgact cgccgatccg ttccgtcgcg gacctgcgcg gccggaccgt
      481 cggcaccggc gccgccgact cgccgcaggc gacgttgatc ccgctgcagc acctcgccga
      541 ggcgggcgtc gagccggaga aggcgtgcac ggtgcggcgc ttcgacgtgc tggtcggcaa
      601 gcacggcgac cacgtcggcg gcgaacgcga cgcggcgcgc gcgctgctgc agggcgaggt
      661 cgacgcggcg tgcatgatcg acggcaacca cctcgcgttc gtcaacgaag gcacgctgcc
      721 ggccggccgc acgcgcgtgc tcgcgcagac cgcgccgtac gaccactgca acttcaccgc
      781 gctcgacggt gcgcccgacg gggcgcccgc ggcgctgatc gcgcgcttcc gcgagctgct
      841 gctggcgatg cgctacgacg atccgcaggt ccggccgctg atggacctcg agggcctcaa
      901 ggcctggcgc gagggccgca cgtccggcta cgcggcgctc gagcgcgcgt gccggcgcac
      961 ggactggctg cagccgttcc tgcgccgcat gggcgcggca tgaccgggac ccgcgtcctc
     1021 gacctcgggc agctcggctt cgaccggggc gcgcacatcc tggtgcagcg cgagctcctc
     1081 cgcctggcgc gcggcgaggc gctggccgtg cgcggcgcgg ccgacgggct ggccgcgcac
     1141 ctgcgcgcgt gggcgcgcgc catgggtcac gagttcgaag cggcgcccgg cctcgccggg
     1201 gtcgtgcgcg ccggccccgc cgccgcggcg cgctggcgtg gcgccgaacg cgcgggctcg
     1261 gccgccgccg acggcgcggt cgcggcggcg ccgctccact ggggcctggc cgcgcgcggc
     1321 gcgctggtcg aggccggcat gcccgcgttc gcgttcgcgc tcgcgcagaa ggacgaggtg
     1381 tgggccgacg acccgggcgc gctctaccgg caggcggcgg cggcgcagtg ggatcccgag
     1441 accgcgatcc cgtgggcggc ggcggccgcg tcgtcgggcg acggcgagga cgcgctcgtg
     1501 caggtgctca cgtacctgat cgagaacgag acggcggcgg cgctggtgcc ggcgcggttc
     1561 tgcgcgcaga cgcacccgca ctaccgcgaa gtcctgcagc tgctcgcgat ccaggccgcc
     1621 gacgaagcgc ggcacatcga ggtgttcacg cgccgcgcgg cgctgcggcg caccgagctg
     1681 ggcacgtcga ccgtcggcgg gcaggcgtcg ctgaagacgc tggtcgacga acccgacttc
     1741 gcgctggcga gcttcctgct gtccgtgctc ggcgagggct cgttcctgca cctgctcggc
     1801 ttcctgcacg tgcacgcgcc cgatccgtgc acggcggaga tcatgcggct cgcggcgcag
     1861 gacgagtcgc gccacgtcgc cttcggcatg gcgcacctgc agcggcacgg cgcccgcgac
     1921 gcgacgctgc gcagccggct cgcgctggcc atcgagcggc ggcacgatgc gctgcggcac
     1981 accgccggac tcgaccagaa cgtcttcgac gcgctggtcg tcctcgccgc gcgcggcttc
     2041 gcccccgagg cgatcgcggg cggacacgcc gcggtcgccg aactggtgca gcagatggac
     2101 gccggacgcc gcgcgcggct gcgcaagctc ggtttcgagg gggccgaggc ggcagcgctc
     2161 agcgcgctgc acacgcgcaa cttcatgtga cgcgacgcgc agccgtccgt tggtcggcgg
     2221 tgcgctcgat cgccgcgagc acggcgcgga aggtggcgag gtcggcggcg gtgaactgac
     2281 ggccgaccgc gcgttcgagg tcgagcacgt ggtcgcgggt gcgcgccgcg acgcgccggc
     2341 ccggggcggt cagctcgatc acgaacgtgc ggcggtcggc ctcgccgacg tcgcgcgtga
     2401 cgagcccgcg ctcgaccagg cggtcgagga tgctggtcag cgtcgagcgc ttgtgcgcga
     2461 gcacttcgtg cagctcgccg acggtggcgg cgcggacctg cgcgagatgg gccagcagat
     2521 gggcttcgcc ctgcgtcagg ccgtgcgcgc gcagcgcgtc caggtagagc cccaccctat
     2581 gcgtggcgcg gtggagcgcg gggatcagct gcaggtgcat gggacggcag gggccgcagg
     2641 tccggcggac gccacgcggc ccgggccgcg tcgccgaaca gcagcgcgcg cgtgtagtag
     2701 gcgagcggca ggtccttgtc cagcagccac ggatgcgcgg ccagcacggc gtcgagcccg
     2761 atcgcatcgc acgccgcggc gcgtacccct gcctgtaccc ctgcaagtag ccgcacccag
     2821 aactgcgtca acgtctcgtg gtagcgctgc gcgctgccgg cacgccgcgc gaacgcgcgg
     2881 atcgcggcgc cgacgcgcgc acacgcggcc tcggtcgacg gtccctcggc gaggcaggcc
     2941 caggcgaggc gcaggtgctc gcggtggcgg aaggagtcgt tgcctacctc gccgcgctcg
     3001 aacgcgcgcg cgaacgccgt gtcgagggcg atggcgtcgt tcatcgcggc ctcgcgtgcg
     3061 ccagcgcgcc ggcgacggcc tgctgccagt gcggtccctg ttgcgcgaag tg
//
LOCUS       DASRDG010000632         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1609896, whole genome shotgun sequence.
ACCESSION   DASRDG010000632 DASRDG010000000
VERSION     DASRDG010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609896"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VFA35_10620"
     CDS             <1..1105
                     /locus_tag="VFA35_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZT56671.1"
                     /translation="SLVRVLDDEGKAVGPWNPNVDAAFLKKGMRAMLRTRTFDARMLV
                     AQRQKKTSFYMQSLGEEAIGCAHEMALERGDMNFPTYRQQSLLLAHDDAPMVEMICQL
                     LSNERDPLKGRQLPVMYSMKRAGFFSVSGNLATQFVQAVGWAMASAIKGDTRIASAWI
                     GDGATAEADFHTALTFAHVYRAPVILNVVNNQWAISTFQAIAGGEGTTFAGRGVGCGI
                     ASLRVDGNDFLAVYAASQWAAERARRNLGPTLIEWLTYRAGPHSTSDDPSKYRPADDV
                     ARFPLGDPIARLKQYLIALGAWSDAQHTALHGELTAEIAAAQKEAEAFGTLADGHVPP
                     LASMFEDVYREMPAHLQAQLRQVQAAKGRAWRP"
     gene            1102..2106
                     /locus_tag="VFA35_10625"
     CDS             1102..2106
                     /locus_tag="VFA35_10625"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZT56672.1"
                     /translation="MTMIQALRSALDVMLARDPNVVVFGQDVGYFGGVFRCTEGLQSK
                     HGSSRVFDTPISEGGIVGIAVGMGAYGLRPVAEIQFADYFYPASDQIVSEAARLRYRS
                     VGDFTAPITIRMPCGGGIYGGQTHSQSPEALFTHVCGLRTLMPSNPYDAKGLLIAAIE
                     CDDPVIFLEPKRLYNGPFDGHHERPVVPWAQHPLAEVPEGHYRVPLESAAVFRAGQAL
                     TVLTYGTMVFVSEAAAVEAGIDAEIIDLRSLWPLDLDAIVASVRKTGRCVIVHEATRT
                     CGFGAELAALVQEHCFYHLEAPIERVTGWDTPYPHALEWDYFPGPARVAAALRRAMED
                     "
     gene            2106..3323
                     /locus_tag="VFA35_10630"
     CDS             2106..3323
                     /locus_tag="VFA35_10630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZT56673.1"
                     /translation="MGVHVIRMPDIGEGIAEVELVVWRVQPGDTVAEDQILADVMTDK
                     ATVEIPSPVAGSVLALGGAEGRVLAVGAELIRIEVADAAGAALAPAPAVVEATAAPRA
                     ATPVPAGPVPGRAAGERPIASPALRRRAWELGIDLHAVAGSGPGGRILQADLAAHAAR
                     APVARHAPAAAPARADEQLQLTGLRRRIAQKMQESKRRIPHYTYVEEVDVTELEALRA
                     QLNARHGAERGRLTLLPLLARAIVLAVGEFPQINARFDDEAGVVTRVAAVHLGIATQT
                     DAGLMVPVLRDAGTRDLWANAAEIARLAGAARAGKALRDELTGSTITLTSLGALGGIV
                     STPVINHPEVAIVGVNRVVARPVIRDGAVVARQMMNLSSSFDHRVVDGMHAAQFVQAL
                     RGLLECPALLFVD"
BASE COUNT          476 a         1201 c         1201 g          465 t
ORIGIN      
        1 ttcgctcgtc cgcgtcctgg atgacgaggg caaggcggtc gggccctgga acccgaacgt
       61 cgacgcggca ttcctgaaga agggcatgcg cgcgatgttg cgcacgcgca ccttcgacgc
      121 gcgcatgctc gtcgcccagc ggcagaagaa gacctcgttc tacatgcaga gcctgggcga
      181 ggaggcgatc gggtgcgcgc acgagatggc gctcgagcgc ggcgacatga acttcccgac
      241 ctaccggcag cagagcctgc tgctcgcgca cgacgacgcg ccgatggtcg agatgatctg
      301 ccagctcctg tcgaacgagc gcgacccgct gaagggccgc cagctgccgg tgatgtactc
      361 gatgaagcgg gccggcttct tctcggtcag cggcaacctc gcgacccagt tcgtccaggc
      421 ggtgggctgg gcgatggcgt cggcgatcaa gggcgacacg aggatcgcct cggcctggat
      481 cggcgacggc gcgaccgccg aggccgactt ccacacggcg ctgaccttcg cgcatgtgta
      541 ccgcgcgccg gtgatcctga acgtcgtcaa caaccagtgg gcgatctcga ccttccaggc
      601 gatcgccggc ggcgaaggca cgaccttcgc cggccgcggc gtcggctgcg gcatcgcgtc
      661 gctgcgcgtc gacggcaacg acttcctggc ggtctatgcg gcctcgcaat gggcggccga
      721 gcgcgcgcgc cgcaacctcg ggccgaccct gatcgagtgg ctcacctacc gcgccggccc
      781 gcactcgacc tcggacgacc cgtcgaagta ccggcccgcc gacgacgtgg cgcgcttccc
      841 gctcggcgat cccatcgcgc gcctcaagca gtacctgatc gcgctcggcg cctggtccga
      901 cgcgcagcac accgcgctgc acggcgagct gacggccgag atcgccgcgg cgcagaaaga
      961 ggcggaggcg ttcggcacgc tcgccgacgg ccacgtgccg ccgctcgcct cgatgttcga
     1021 ggatgtctac cgcgagatgc cggcgcacct gcaggcgcag ctgcggcaag tgcaagcggc
     1081 gaagggccga gcatggcggc catgacgatg atccaggcgc tgcgctcggc gctggacgtg
     1141 atgctggcgc gcgacccgaa cgtggtcgtg ttcggccagg acgtgggcta cttcggcggc
     1201 gtgttccgct gcaccgaggg cctgcaatcg aagcacggca gctcgcgcgt gttcgacacg
     1261 ccgatctccg agggcggcat cgtcggcatc gcggtcggca tgggcgccta cgggctgcgg
     1321 ccggtcgccg agatccagtt cgccgactac ttctacccgg cctcggacca gatcgtctcc
     1381 gaggcggcgc ggctgcgcta ccgctcggtc ggcgacttca ccgcgccgat cacgatccgc
     1441 atgccctgcg gcggcggcat ctacggcggc cagacccaca gccagagccc cgaggcgctg
     1501 ttcacgcacg tgtgcgggct gcgcacgctg atgccgtcga atccgtacga cgcgaaaggc
     1561 ctgctgatcg cggcgatcga gtgcgacgac ccggtgatct ttctcgaacc gaagcgtctg
     1621 tacaacgggc cgttcgacgg ccaccacgag cgccccgtcg tgccctgggc ccagcacccg
     1681 ctggccgagg tgcccgaggg ccactaccgc gtgccgctcg aatcggccgc ggtgttccgc
     1741 gccgggcagg cgctgacggt gctgacctac ggcacgatgg tgttcgtcag cgaggccgct
     1801 gccgttgaag ccggcatcga cgccgaaatc atcgacctgc gcagcctgtg gccgctggac
     1861 ctggacgcaa tcgtggcctc ggtcaggaag accgggcgct gcgtgatcgt gcacgaggcg
     1921 acgcgcacct gcggcttcgg cgccgaactg gcggcgctgg tgcaggagca ctgcttctac
     1981 cacctcgagg caccgatcga gcgcgtgacc ggctgggaca cgccgtaccc gcacgcgctg
     2041 gagtgggact actttcccgg cccggcgcgc gtggccgcgg cgctgcgcag ggcgatggag
     2101 gactgatggg cgtgcacgtg atcaggatgc ccgacatcgg cgaaggcatc gccgaggtcg
     2161 aactcgtcgt ctggcgcgtg cagcccggcg acacggtcgc cgaggaccag atcctcgccg
     2221 acgtgatgac cgacaaggcg accgtcgaga ttccgtcgcc ggtggccggc agcgtgctcg
     2281 cgctcggcgg cgccgaaggc cgggtgctcg cggtgggcgc cgagctgatc cgcatcgagg
     2341 tcgccgacgc ggcgggcgcc gcgctggcgc cggcgcccgc ggtcgtcgag gccaccgccg
     2401 cgccgcgtgc cgcaacaccg gtgccggccg ggccggttcc cggccgcgcg gccggcgagc
     2461 ggccgatcgc ctcgccggcg ctgcgccggc gcgcctggga gctcggcatc gatctgcacg
     2521 cggtcgccgg cagcggcccg ggcgggcgca tcctgcaggc cgatctggcc gcgcatgcgg
     2581 cacgcgcgcc cgttgcgcgg catgcccccg cggccgcacc ggcgcgcgcc gacgaacagc
     2641 tgcagctcac cgggctgcgc cgccggatcg cgcagaagat gcaggagtcc aagcgccgca
     2701 tcccgcacta cacctatgtc gaagaggtcg acgtgaccga gctcgaggcg ttgcgtgcgc
     2761 agctcaacgc caggcacggc gccgagcgcg gccgcctgac cttgctgccg ctgctggcgc
     2821 gggcgatcgt gctcgcggtc ggcgagttcc cgcagatcaa cgcgcgcttc gacgacgagg
     2881 ccggcgtcgt cacgcgcgtg gccgcggtgc acctcggcat cgcgacgcag accgacgccg
     2941 ggctgatggt gccggtgctg cgcgacgccg gcacgcgcga cctgtgggcc aacgccgccg
     3001 agatcgcacg cctggccggg gccgcgcgcg ccggcaaggc gctgcgcgac gaactgaccg
     3061 gctcgaccat caccttgacc agcctgggcg cgctcggcgg catcgtctcg acgccggtga
     3121 tcaaccaccc ggaagtggcg atcgtcggcg tcaaccgcgt cgtcgcgcgg ccggtgatcc
     3181 gcgacggcgc ggtcgtcgcg cgccagatga tgaacctgtc gtcgtcgttc gatcaccgcg
     3241 tcgtcgacgg catgcacgcg gcgcagttcg tgcaggcgct gcgtggcctg ctcgaatgcc
     3301 cggcgctgtt gttcgtcgac tgactccgct tgccgccgct att
//
LOCUS       DASRDG010000633         4989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1250663, whole genome shotgun sequence.
ACCESSION   DASRDG010000633 DASRDG010000000
VERSION     DASRDG010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250663"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1534
                     /locus_tag="VFA35_10635"
     CDS             41..1534
                     /locus_tag="VFA35_10635"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HZT56674.1"
                     /translation="MNEASARAVLLLQAFETAQPPSPSWSDDDRAWATRLALDAAAPD
                     AETFIAQRARHAMQRLAPREPAAASWLARRLWRARWVGWAVLAGLVVGALADSIGSGQ
                     RINLLAPPLWAVLAWNALVYLVLLGHGLARLLMRPTRPGSLVLLTQRLLRLGRRLPGP
                     GAASASADGPSGSARALQAFATGWLRASAPLAAARASTLLHAAAAALALGLIAGMYLR
                     GLVLDYRVAWESTFLSAETAHAALAALLAPAVAISGIALPDAAGFEALRAVHGGAAAS
                     ASAAPWIHLLALTLLLFVVLPRSALALGAALRAHWLARHIALPLADAYFQRLARLQRG
                     DVAHVVVQPYAATPSAQAALGLRSLLAPALGDGLQLRVAPTAAFGAEDADDSAAALDA
                     GTTLAIALFDLAATPEAESQGRFAQQLAQRAPAGAATILLVDEAGFRQRFGADSARLA
                     QRRDAWRVFGEALGTLPVCVDLGAPDVPGATRALQLALRSPLNRGTA"
     gene            1531..3144
                     /locus_tag="VFA35_10640"
     CDS             1531..3144
                     /locus_tag="VFA35_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HZT56675.1"
                     /translation="MSAGRAPGRPQAGPHPLGGSIAVSGDRGAHNISLSLVSHTNAGK
                     TTLARTLLGRDVGTVRDAPHVTEFADVFTMLETPQRERLLLWDTPGFGDSLRLVRRLR
                     GAANPVGWLVAEVWDRWRDRPFWASQQAMKNVRDEADVMLYLVNAAESPEAAGYVAPE
                     MELLGWTAKPVIVLLNQLGAPRDGAAEDADLLRWRAYLAQWPQVRAVLPLDAFARCWV
                     QEGTLLQAIEAALPPPRRALMARLRAAWSARRAQVFDASMASLAASLARLAGAFEEVP
                     DPATLAARLRRVGHAIGIGGSDETPLALAQKALAERLDAEVRANTAELIALHGLEGQA
                     QGEILTRLATHFETRLRMDEGKAALWGGMVSGALVGLKADILSGGLTLGGGLLAGGLI
                     GALGAAGLARCVNLVRGTDRSFVAWNAQALDQLLEAALLRYLAVAHFGRGRGDWAQGE
                     APPHWRELIQHALSPQRDALALIWNERDAHGEAGEGDKSAERGAGERLIAAALVPVLC
                     EAAAQTLRTLYPNAPPLRESAQPVAVPAQ"
     gene            3155..3766
                     /locus_tag="VFA35_10645"
     CDS             3155..3766
                     /locus_tag="VFA35_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HZT56676.1"
                     /translation="MNAPHRPIDFYFDFSSPYSYIASEWIEALAARHGRTVAWRAILL
                     GATFQVTEIKSPVSYPLKREYTMLDFERSARFAGVPLKVPAKFPIATQNTARIFWWLN
                     ADDPARARDWARHALRALFARGVDLSDAAALRALAIEFGLDGSQAADVWNDPHWKAAL
                     KDANDAAIALGVFGAPFFMIDGQPFWGNDRRPMIERWLEKGPF"
     gene            3856..>4989
                     /locus_tag="VFA35_10650"
     CDS             3856..>4989
                     /locus_tag="VFA35_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZT56677.1"
                     /translation="MPALASKLSPRSEDFKANAAAMRALVDDLNAKLAQIALGGGEGP
                     RAKHVARGKLLPRDRVEMLLDPDTPFLEIAPLAALEMYRNDAPGAGLIAGIGRVSGVE
                     CMIVCNDATVKGGTYYPMTVKKHLRAQEIAQQNRLPCIYLVDSGGANLPNQDEVFPDR
                     EHFGRIFFNQAQMSAQGIPQIAVVMGSCTAGGAYMPAMSDESIIVKNQGTIFLAGPPL
                     VKAAIGEIVSAEELGGGDVHTRLSGVADHLAQNDTHALALARASVANLNWRKTHEQRL
                     IEPRAPLFDPAELHGVIPTDTRKPYDVREIIARIVDGSEFDEFKARYGTTLVTGFAHI
                     HGHPVGIVANNGILFSESALKGAHFIELCGQRKVPLVFLQNITG"
BASE COUNT          643 a         1820 c         1851 g          675 t
ORIGIN      
        1 cgatctcgcc cgccgcctcg gcgcctgacg ccgcaagccg atgaacgaag ccagcgcgcg
       61 cgccgtcctg ctgctgcagg ccttcgagac cgcgcagcca ccgagcccga gctggagcga
      121 cgacgaccgc gcgtgggcga cccggctcgc gctcgacgcc gccgcgcccg acgccgagac
      181 cttcatcgcg cagcgcgcac gccatgcgat gcagcgcctg gcgccgcgcg agcccgcggc
      241 ggcgagctgg ctggcgcgcc ggctgtggcg agcgcgctgg gtcggctggg cggtgcttgc
      301 cgggctcgtc gtcggcgcgc tcgccgacag catcggcagc ggtcagcgca tcaacctgct
      361 cgcgccgccg ctgtgggcgg tgctggcgtg gaacgcgctc gtctacctcg tgctgctcgg
      421 ccacggcctg gcgcggttgc tgatgcgccc gacccggccc ggcagcctgg tgctgctcac
      481 gcagcgcctg ctgcgcctcg gccgccgcct gccggggccg ggcgcggcga gtgcgtcggc
      541 cgatggcccg agcggcagcg cgcgcgcgct gcaggccttt gcgaccgggt ggctgcgcgc
      601 gagtgcgccg ctcgcggcgg cgcgtgcgtc gaccctgctg cacgccgccg ccgccgcgct
      661 cgcgctcggg ctgatcgccg gcatgtacct gcgcggcctg gtgctcgact accgcgtcgc
      721 gtgggagagc actttcctgt cggccgagac ggcgcacgcg gcgctcgcgg cgttgctcgc
      781 gccggccgtg gcgatctcgg gcatcgcctt gcccgatgcg gccgggttcg aggcgctgcg
      841 cgcggtgcac ggcggcgccg cggccagtgc ctcggccgcg ccgtggatcc acctgctcgc
      901 gctgaccctg ttgctgttcg tcgtgctgcc gcgcagcgcg ctcgcgctcg gcgcggcgct
      961 gcgcgcgcac tggctggcgc ggcacatcgc gctgccgctg gccgatgcgt acttccagcg
     1021 gctcgcgcgg ctgcagcgcg gcgatgtcgc gcatgtcgtc gtccagccct acgccgcgac
     1081 gccgagcgcg caggccgcgc tcgggctgcg ctcactgctc gcgccggcgc tgggcgacgg
     1141 gctgcagctg cgtgttgcgc cgacggcggc gttcggcgcc gaggacgccg acgacagcgc
     1201 cgccgcgctc gacgccggca cgacgctcgc gatcgcgctg ttcgacctcg cggccacgcc
     1261 cgaggccgag agccagggcc gtttcgcgca gcaactcgcg cagcgcgcgc cggccggcgc
     1321 ggcgacgatc ctgctggtcg acgaggccgg gttcaggcag cgcttcggcg ccgactcggc
     1381 gcggctcgcg cagcgccgcg acgcgtggcg cgtgttcggc gaggcgctgg gcacgctacc
     1441 ggtgtgcgtc gacctcggcg cgcccgacgt gcccggcgcg acgcgtgcgt tgcagctcgc
     1501 gctgcgcagc ccgttgaacc ggggaacagc gtgagcgcgg gccgagcgcc gggccgcccc
     1561 caagccggcc cgcatcccct cgggggatcg atcgccgtat ccggcgatcg aggggcccac
     1621 aacatttcgc tcagcctcgt ctcgcacacg aacgccggca agaccacgct cgcgcgcacc
     1681 ttgctcggcc gcgatgtcgg cacggtgcgc gacgcgccgc acgtcaccga gttcgccgac
     1741 gtgttcacga tgctcgagac gccgcagcgc gagcggctgc tgctctggga cacgccgggc
     1801 ttcggcgaca gcctgcggct cgtgcgccgc ctgcgcggcg cggccaaccc tgtcggctgg
     1861 ctcgtcgccg aggtctggga ccgctggcgc gaccggccgt tctgggccag ccagcaggcg
     1921 atgaagaacg tgcgcgacga ggccgacgtg atgctgtacc tcgtcaacgc cgcggagtcg
     1981 cccgaggcgg ccggctatgt cgcgcccgag atggagttgc tcggctggac ggccaagccg
     2041 gtgatcgtgc tgctgaacca gctcggcgcg ccgcgcgacg gcgcggccga agacgccgac
     2101 ctgctgcgct ggcgtgccta cttggcgcag tggccgcaag tgcgcgcggt gctgccgctc
     2161 gacgcgttcg cgcgctgctg ggtgcaggag ggcactttgc tgcaggcgat cgaggcggcg
     2221 ctgccgccgc cgcgccgcgc gctgatggcg cggctgcgcg cggcgtggtc ggcgcggcgc
     2281 gcgcaggtgt tcgacgcatc gatggcctcg ctcgcggcca gcctggcgcg cctggccggc
     2341 gcgttcgagg aagtgcccga cccggcgacg ctggctgcgc ggctgcgacg cgtcggccat
     2401 gcgatcggca tcggcggttc ggacgagacg ccgctcgcgc tggcgcagaa ggcgctcgcc
     2461 gagcgcctcg atgccgaggt gcgcgcgaac accgccgagc tgatcgcgct gcacgggctc
     2521 gagggccagg cgcagggcga gatcctgaca cggctcgcaa cgcatttcga gacgcggctg
     2581 cgcatggacg aaggcaaggc ggcgctgtgg ggcggcatgg tcagcggtgc gctggtcggg
     2641 ctgaaggccg acatcctgag cggcggcctg acgcttggcg gcggcctgct ggccggcggc
     2701 ctgatcggcg cgctcggcgc cgccggcctg gcgcgctgcg tgaacctggt gcgcggcacc
     2761 gatcgctcgt tcgtcgcgtg gaacgcgcag gcgctcgacc agttgctcga ggccgcgctg
     2821 ctgcgctacc tcgcggtcgc gcacttcggc cgcggccgcg gcgattgggc gcaaggcgag
     2881 gcgccgccgc actggcgcga gctgatccag cacgcgctgt cgccgcagcg cgacgcgctg
     2941 gcactgatct ggaacgagcg tgatgcgcac ggcgaggccg gcgaaggtga caagagcgcc
     3001 gagcgcggcg cgggcgaacg cctgatcgcc gccgcgctcg tgcccgtgct gtgcgaggcg
     3061 gcggcgcaga cgctgcgcac gctctacccg aacgcgccgc cgcttcggga aagtgcccag
     3121 cccgtggcgg tgccggcgca gtagcctagg gcggatgaac gccccgcacc gaccgatcga
     3181 tttctacttc gacttctcgt cgccgtactc ctacatcgcc tccgagtgga tcgaggcgct
     3241 ggcggcgcgc cacggccgca cggttgcatg gcgcgcgatc ctgctcggcg cgaccttcca
     3301 ggtcaccgag atcaagagcc cggtgtcgta cccgctgaag cgcgagtaca cgatgctcga
     3361 cttcgagcgc tcggcgcgtt tcgccggcgt gccgctgaag gtgccggcga agttcccgat
     3421 cgcgacgcag aacaccgcgc gcatcttctg gtggctgaac gccgacgacc cggcgcgcgc
     3481 tcgcgattgg gcgcggcatg cgctgcgcgc gctgttcgcc cgcggcgtcg acctgagcga
     3541 cgcggccgcg ctgcgcgcgc tggcgatcga gttcggcctc gacggctcgc aggcggcgga
     3601 cgtctggaac gacccgcact ggaaggccgc gctgaaggac gcgaacgacg ccgcgatcgc
     3661 gctcggcgtg ttcggcgcgc cgttcttcat gatcgacggc cagcccttct ggggcaacga
     3721 ccggcgcccg atgatcgagc gctggctcga gaaggggccg ttctagcggg tcgaaccggc
     3781 ggccccggcc gagccctaca attgacgtat acgtcaactg cgaagttcgg cgcgcccggc
     3841 gccacgaaag cccgcatgcc cgcactcgcc tccaagctca gcccgcgctc cgaagacttc
     3901 aaggccaacg ccgcggcgat gcgcgcgctg gtcgacgact tgaacgcgaa gctcgcgcag
     3961 atcgcgctcg gcggcggcga ggggccgcgc gccaagcacg tcgcgcgcgg caagctgctg
     4021 ccgcgcgacc gggtcgagat gctgctcgac cccgacacgc cgttcctcga gatcgcgccg
     4081 ctggccgcgc tcgagatgta ccgcaacgac gcgcccggcg ccgggctgat cgccggcatc
     4141 ggccgcgtca gcggtgtcga gtgcatgatc gtgtgcaacg acgcgacggt gaagggcggc
     4201 acctactacc cgatgacggt gaagaagcac ctgcgcgcgc aggagatcgc gcagcagaac
     4261 cggctgccgt gcatctacct cgtcgacagc ggcggcgcga acctgccgaa ccaggacgag
     4321 gtgttccccg accgcgagca cttcggccgc atcttcttca accaggcgca gatgagcgcg
     4381 cagggcatcc cgcagatcgc ggtcgtgatg ggctcgtgca cggccggcgg cgcctacatg
     4441 ccggcgatga gcgacgagtc gatcatcgtc aagaaccagg gcacgatctt cctggccggt
     4501 ccgccgctcg tgaaagcggc gatcggcgaa atcgtcagcg ccgaggagct gggcggcggc
     4561 gacgtgcaca cgcggctgtc cggcgtggcc gatcacctgg cgcagaacga tacgcacgcg
     4621 ctcgcgctgg cgcgcgcttc ggtcgcgaac ctgaactggc gcaagacgca cgagcagcgc
     4681 ctgatcgagc cgcgcgcgcc gctgttcgat ccggccgagc tgcacggcgt gatcccgacc
     4741 gacacgcgca agccgtacga cgtgcgcgag atcatcgcgc gcatcgtcga cggctccgag
     4801 ttcgacgagt tcaaggcacg ctacggcacg acgctggtca ccgggttcgc gcacatccac
     4861 gggcatccgg tcgggatcgt ggccaacaac gggatcctgt tctccgagtc ggcgctgaag
     4921 ggcgcgcact tcatcgagct gtgcgggcag cgcaaggtgc cgctcgtctt cctgcagaac
     4981 atcaccggc
//
LOCUS       DASRDG010000634         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1250669, whole genome shotgun sequence.
ACCESSION   DASRDG010000634 DASRDG010000000
VERSION     DASRDG010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250669"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /gene="nrfD"
                     /locus_tag="VFA35_10655"
     CDS             <1..1159
                     /gene="nrfD"
                     /locus_tag="VFA35_10655"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZT56678.1"
                     /translation="PVMWAVDITNFVFWVGIAHCGTLVSAVLFLFRSHFRRAVYRIAE
                     AMTVFGVLTAAMFPAIHTGRPWFDYWLFPYPNQRQLWTNVRSPLEWDVFAVNTYLTVS
                     SIFFLVGLIPDVAAVRDRTKHPLMKFIYTLLSFGWTGANYQWKHFYGAYLFFAALATP
                     LVFSVHSVVSWDFAMAQVPGWHSTLFAPYFVAGAIFSGVALVINLLIPIRKAFKLEHI
                     VTLDHMEKLGKLVLLTSCMVGYSYLTEFFMAWFGPDKAEGDVFWFRATGYYWWCFWTM
                     FSCNVIFPLLLWFKSFRRNMFALFILCIFVNVGMWFERFVIIVTSLTRAFNPANWFYY
                     HISITEVALIVGSFAWFFMFFLVFVRVLPAFSIAEIKETLPAPRRGDGGHR"
     gene            1156..1701
                     /locus_tag="VFA35_10660"
     CDS             1156..1701
                     /locus_tag="VFA35_10660"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HZT56679.1"
                     /translation="MIGTIQKLLFPPPPKFYGVMGIYYYVDDAAKAVTSLRQAGHKHL
                     RVYSPVPHHEIEAALEQGPSLVRWVTAVGGFLGCTGGFALCWYTIYTWPMVIGGKELY
                     SVPPFTILGYESMILLAGLSNLFGMLALARLPEIRATAPYDPRFQEDRIGIWVACPPD
                     KAAPIEQTMKGHGAEEVQVHA"
     gene            1694..>2169
                     /locus_tag="VFA35_10665"
     CDS             1694..>2169
                     /locus_tag="VFA35_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56680.1"
                     /translation="MLRPIAVLGLLALWSVSVQQGCNDLKKAVTHLKYYPIRDMRQTI
                     APDPQRDIWSSTGPVRWAAVPDSLSVPTIGVDAYAAATAPYDAVEKKIVAPPATDASI
                     AHGDSLFHTICWSCHGKTMAGDGPIAAKFIPPPDLLAESSRGKTDGFMYMYMRHGGA"
BASE COUNT          311 a          748 c          690 g          420 t
ORIGIN      
        1 cccggtgatg tgggcggtcg acatcacgaa cttcgtgttc tgggtcggca tcgcgcactg
       61 cggcacgctg gtgtcggcgg tgttgttcct gttccgctcg cacttccggc gcgccgtcta
      121 ccgcatcgcc gaggcgatga ccgtgttcgg cgtgctcacc gccgccatgt tccccgccat
      181 ccacaccggc cggccgtggt tcgactactg gctgttcccg tacccgaacc agcgccagct
      241 gtggacgaac gtccgctcgc cgctggagtg ggacgtgttc gccgtcaaca cgtacctgac
      301 cgtgtccagc atcttcttcc tggtcggcct gatccccgac gtggcggccg tgcgcgaccg
      361 caccaagcac ccgctcatga agttcatcta cacgctgctg tcgttcggct ggaccggcgc
      421 caactaccag tggaagcact tttacggggc gtatctcttc ttcgcggcgt tggccacgcc
      481 gctggtgttc tcggtgcact cggtcgtgtc gtgggacttc gcgatggcgc aggtgccggg
      541 ctggcactcg acgctgttcg cgccctactt cgtggccgga gcgatcttct ccggcgtcgc
      601 gctggtgatc aacctgctga tccccatccg caaggcgttc aagctcgagc acatcgtcac
      661 gctcgaccac atggagaagc tcgggaagct cgtgctgctc acctcgtgca tggtgggcta
      721 ctcctacctg accgagttct tcatggcgtg gttcggcccc gacaaggccg agggcgacgt
      781 gttctggttc cgcgccaccg gctactactg gtggtgtttc tggaccatgt tctcgtgcaa
      841 cgtgatcttc ccgctgctgc tgtggttcaa gagtttccgc cgcaacatgt tcgccctgtt
      901 catcctctgc atcttcgtga acgtgggcat gtggttcgag cgcttcgtga tcatcgtcac
      961 ctcgctcacg cgtgcgttca atcccgcgaa ctggttctac taccacatca gcatcaccga
     1021 ggtcgcgctc atcgtcggct cgttcgcgtg gttcttcatg ttcttcctcg tgttcgtgcg
     1081 cgtgctgccc gcgttctcga tcgccgagat caaggagacg ctgccggcgc cgcgccgtgg
     1141 ggacgggggc caccggtgat cgggaccatc cagaagctgc tgttcccgcc gccgcccaag
     1201 ttctacggcg tcatgggcat ctactactac gtggacgacg cggcgaaggc cgtgacctcg
     1261 ctccgccagg ccgggcacaa gcacctgcgc gtctactcgc cggtgccgca ccacgagatc
     1321 gaggcggcgc tcgagcaggg cccgtcgctc gtgcgctggg tgacggccgt gggcggcttc
     1381 ctcggctgca ccggcggctt cgcgctgtgc tggtacacga tctacacgtg gccgatggtg
     1441 atcggtggca aagagctcta cagcgtgccg ccgttcacga tcctcggcta cgagagcatg
     1501 atcctgctcg ccggcctctc gaacctgttc ggcatgctgg cgctggcgcg gctgcccgag
     1561 atccgcgcca cggcgccgta cgacccgcgc ttccaggagg accgcatcgg catctgggtg
     1621 gcctgcccgc ccgacaaggc cgcgcccatc gagcagacga tgaaaggcca cggcgccgag
     1681 gaggtccagg tccatgctta ggccgatcgc cgtgctcggg ttgctcgcgc tgtggtcggt
     1741 gtcggtgcag cagggctgca acgacctgaa gaaggccgtc acgcacttga agtactaccc
     1801 gatccgcgac atgcggcaga cgatcgcgcc cgacccgcag cgcgacatct ggtcctcgac
     1861 cgggccggtg cgctgggccg cagtgcccga ctcgctgagc gtgccgacga tcggggtcga
     1921 cgcgtacgcg gccgccaccg cgccgtacga tgcggtggag aagaagatcg tggcgccgcc
     1981 cgcgaccgac gcgtcgatcg cgcacggcga cagcctgttc cacaccatct gctggtcgtg
     2041 ccacggcaag accatggccg gcgacgggcc catcgccgcc aagttcattc cgccgcccga
     2101 cctgctcgcc gagagctcgc gcggcaagac ggacggcttc atgtacatgt acatgcgcca
     2161 cggcggcgc
//
LOCUS       DASRDG010000635         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_669952, whole genome shotgun sequence.
ACCESSION   DASRDG010000635 DASRDG010000000
VERSION     DASRDG010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669952"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2844
                     /locus_tag="VFA35_10670"
     CDS             <1..2844
                     /locus_tag="VFA35_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HZT56681.1"
                     /translation="LDVRGLPQELPPNAFQLKLVDASGENVWWGQRSEFRFPTTWQTL
                     RFRQRQIEFAWGPASDKALRKTASIELVIASGTGAGKGSACVDRLTLRRLPDVAPPTP
                     APVMRADGLSVDYGLPREFGALALRWRPGAAGSRYAIELSDDGASWRRVREVERARGQ
                     VQTHWLPESEARFVRVVPEAGRAVALDAIEPRDAPSANAFFMQLAKDAPRGRYPRAYV
                     GEQSYWTVLGVDGARVASLLSEDGVLEPVPGVGALEPFLVADGRVLSWADARITHRLR
                     DGDLPMPAVHWLAKDLALDIEAFGHGTPAAAQALARYTVHNLGRTTRRVTLALAWRPF
                     QANPPTQFLAHPGGASAVDSIAWDGRALRVNGATRVQPLVAPTSVQLEPLAAGPVGDW
                     LAESTRVAQVADAAGFANGVLRYELRLRPGESRVVTVALPVSGAPVAPTLASLARAER
                     LVAAQWRERLGRVRISGPGEVADVAATLRTALGHILVNRSGPALQPGARAYARSWIRD
                     GALTSSALLRLGHADAARDFLQWFAPFQFKNGKVPCCATARGADPVPENDSDGEFAFA
                     VADLWQYTRDEQAARALWPNVRAAVAHMESLRQSERTDKNRPPERSEGSQPTERVAYF
                     GLMPPSISHEGYADKAAYSYWDDFWAAIGYRSAVELAQALNLPDDAARMGAQRDEFLA
                     DLQASIAASTQRFGLAVLPGAADRGDVDPTSSNVALSPGGLLGTLPDALVRQTFERYW
                     GEFEARRRSEAAGVRHGDGAYTPYEWRNVGAFVRLGQRERALAAMAYFYRDRRPAGWK
                     QWAEVVVRDAREPRFLGDMPHGWVASDQIRAVLDLFAYEHEGAHALVLAAGVPLAWLD
                     GPGLKIDGLRTPYGPLGWQARARTIAGRTTVTFDLKPLRTQPPGGIELRGPWPANARV
                     WIDGRPVATPADRIALPRTPVRVRIELPEPGRG"
     gene            2896..3216
                     /locus_tag="VFA35_10675"
     CDS             2896..3216
                     /locus_tag="VFA35_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56682.1"
                     /translation="MPRRPVLALVFASSLLAGCDMLGGALGIESPEKAAAAREADGRA
                     IGGACRHAGRAIEDCYALNRKADRAAVFAGWRDMNDYMRENKIDAVAPQLGTQVAGAK
                     AHDS"
     gene            3338..4138
                     /locus_tag="VFA35_10680"
     CDS             3338..4138
                     /locus_tag="VFA35_10680"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HZT56683.1"
                     /translation="MRSTHTVFLRRIAALLVVASASAVISTPARADDDGKVHRSFFIN
                     GGGARVTGSGKLASENRAVAGFQAIALRASMKLVLRQGPREGIELRADDNILSLIETV
                     VVERGGVPTLEIAAKKGTNFSTGNPIVATIDLVTLRALSISGSGDVVGDGLKTPGLSV
                     AISGSGNVRLKQLVADEVSARVSGSGDIEFSGRTAKLGVSISGSGDVKTRGLEADDVS
                     VSVAGSGDADVTARRTLTVSIAGVGNVKYSGDATVKSSIAGRGNVTKQ"
     gene            4169..4912
                     /locus_tag="VFA35_10685"
     CDS             4169..4912
                     /locus_tag="VFA35_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZT56684.1"
                     /translation="MSEEVLNRRPVWREPEFARGVRDMVGVSLGIAAWGLVTGVAMAK
                     SGLPLPLCILMSLLVFAGSAQLAALPMLVSGAPVWMSWAIALCVNLRFLIFSAQWRPY
                     WSHLSRGQRMLRGYFTGDLNYVIFMTRFPEPRPAPAQEPYFWGGVATNWSAWQIPSLA
                     GMLLADAIPTEWGFGFAGILALLGLMYSLMHDRASWVAAGVASAAAVAAAALPLKLNV
                     VVAIAAAIAVGLLMDHATPVEARPMEDNT"
BASE COUNT          654 a         1782 c         1837 g          702 t
ORIGIN      
        1 ctcgacgtgc gcggcctgcc ccaggaactg ccgccgaacg cgttccagct caagctggtc
       61 gacgcgagcg gcgagaacgt ctggtggggt cagcggagcg agttccgctt cccgaccacg
      121 tggcagacat tgaggttccg ccagcgccag atcgagttcg cgtggggccc ggcgagcgac
      181 aaggcgctgc gcaagacggc gtcaatcgag cttgtcatcg catcgggcac cggcgcgggc
      241 aagggcagcg cgtgcgttga ccggctgacc ttgcgccgac tgccggacgt ggcgccgccg
      301 acccccgcgc cagtgatgcg cgccgacggc ctgagcgtcg actacgggct gccgcgcgag
      361 ttcggcgcgc tcgcgttgcg ctggcgcccc ggcgcggccg gctcgcgcta cgccatcgag
      421 ctgtcggacg acggcgcgtc atggcgccgc gtgcgcgagg tcgaacgcgc gcgcggccag
      481 gtgcagacgc actggctgcc cgagtccgaa gcgcgcttcg tgcgcgtcgt gccggaggcc
      541 ggccgcgccg tggcgctcga tgcgatcgag ccgcgcgacg cgccgagcgc gaacgcgttc
      601 ttcatgcagc ttgcgaagga cgcgccgcgc ggccgctacc cgcgtgccta tgtcggcgag
      661 cagtcgtact ggaccgtgct cggcgtcgac ggcgcgcgcg tcgcgtcgct gctgagcgag
      721 gacggcgtcc tcgagccggt gcccggcgtc ggcgcactcg agccctttct cgtcgccgac
      781 ggccgggtgc tgagctgggc ggacgcgcgg atcacccacc gtctgcgcga cggcgatctg
      841 ccgatgcccg cggtgcactg gcttgcgaag gacctcgcgc tcgacatcga ggccttcggc
      901 cacggcacgc ccgccgccgc gcaggcgctc gcgcgctaca ccgtgcacaa cctcggccgc
      961 acgacgcgca gggtcacgct cgcgctcgcg tggcggccgt tccaggcgaa cccgccgacg
     1021 cagtttctcg cccacccggg cggcgcgagc gcggtcgatt cgatcgcgtg ggacggccgc
     1081 gcgctgcgcg tcaacggcgc gacgcgcgtg cagccgctgg tcgcgccgac gagcgttcag
     1141 ctggagccgc tcgcggccgg gcccgtcggc gactggctcg ctgaatccac gcgcgtcgca
     1201 caggtcgccg acgcggcggg cttcgcgaac ggcgtgctgc gctacgaact gcgcctgcgg
     1261 ccgggcgagt cgcgcgtggt cacggtcgca ttgccggtca gcggcgcgcc ggtcgcgccg
     1321 acgctcgcca gcctggcccg cgcggagcgg cttgttgccg cgcagtggcg cgagcgcctc
     1381 ggccgcgtgc gcatcagcgg cccgggcgag gttgccgacg tggccgcgac gctgcgcacc
     1441 gccctcggcc acatcctcgt gaaccgcagc gggccggcgc tgcagcccgg cgcgcgcgcc
     1501 tatgcgcgct cgtggatccg cgacggcgcg ctgacctcgt cggcgctgct gcgcctgggc
     1561 cacgccgacg cggcgcgcga cttcctgcag tggttcgcgc cgttccagtt caagaacggc
     1621 aaggtgccgt gctgcgcgac cgcgcgcggc gccgacccgg tgcccgagaa cgacagcgac
     1681 ggcgagttcg cgttcgcggt cgccgatctg tggcagtaca cacgcgacga gcaggccgcg
     1741 cgcgcgctgt ggccgaacgt gcgcgcggcc gtggcgcaca tggagtcgct gcgccaaagc
     1801 gagcgcaccg acaagaaccg cccacccgag cgcagcgagg gttcccagcc cacggagcgc
     1861 gtcgcctact tcgggctgat gccgccgtcg atcagccacg agggctatgc cgacaaggcg
     1921 gcctactcgt actgggacga cttctgggcc gccatcggct accgcagcgc ggtcgaactc
     1981 gcgcaggcgc tgaacctgcc cgacgacgca gcgcgcatgg gcgcgcagcg cgacgagttc
     2041 ctcgccgacc tgcaggcctc gatcgccgcg agcacgcagc gcttcggcct cgccgtgctg
     2101 cccggcgcgg ccgaccgcgg cgatgtcgac ccgacctcga gcaacgtcgc gctgagcccc
     2161 ggcggcctgc tcggcacctt gcccgacgcg ctggtgcgcc agaccttcga gcgctactgg
     2221 ggcgagttcg aggcgcgccg gcgcagcgaa gccgccggcg tgcgccacgg cgacggtgcc
     2281 tacacgccgt atgagtggcg caatgtcggc gcgttcgtgc gcctcggcca gcgcgagcgg
     2341 gcgctggcgg cgatggccta cttctaccgc gaccgccgcc cggccggctg gaagcagtgg
     2401 gccgaggttg tcgtgcgcga tgcgcgcgag ccgcgctttc tcggcgacat gccgcacggc
     2461 tgggttgcct cggaccagat ccgcgcggtg ctcgacctgt tcgcttacga gcacgagggc
     2521 gcgcacgcgc tggtgcttgc ggccggcgtg ccgctggcgt ggcttgacgg cccgggcctg
     2581 aagatcgacg gcctgcgcac gccctatggc ccgctcggct ggcaggccag ggcgcgcacg
     2641 atcgcggggc gcacgacggt caccttcgac ctgaagccgc tgcgcacgca gccgcccggc
     2701 ggcatcgagc tgcgcgggcc atggccggcg aatgcgcgcg tgtggatcga cggccgcccg
     2761 gtcgcgacgc cggccgaccg gatcgcgctg ccgcgcacgc cggtgcgggt gcgcatcgag
     2821 ctgcccgagc cggggcgcgg ctgaaccgcg ccaactcgcg ctcaagccgg cgccgggcgc
     2881 gccgataagc tcgccatgcc ccgccgtccc gtcctcgccc tcgtcttcgc cagcagcctg
     2941 ctcgcgggtt gcgacatgct gggcggcgcg ctgggcatcg agtcgcccga gaaggcggcg
     3001 gccgcgcgcg aggccgacgg ccgtgcgatc ggcggcgcgt gccgccacgc cggccgcgcg
     3061 atcgaagact gctatgcgct gaacaggaag gccgaccgcg ccgcggtgtt cgccggctgg
     3121 cgcgacatga acgactacat gcgcgagaac aagatcgatg ccgtcgcgcc gcagctcggc
     3181 acccaggtgg ccggcgcgaa ggcgcacgat tcgtaacctg ccgcagcccg cccgcaaccc
     3241 acgtttcgtc gcgcgaggct gcagttcgtc gcgcgccgcg tgacggcgcc tcggggcgcg
     3301 ggcacagtcg cgtctcccgt tccactgcgg agacaccatg cgttccaccc acaccgtttt
     3361 cctccgccgc atcgcggcgc tgctggtcgt cgcgagcgcg agcgccgtga tctcgacccc
     3421 ggcccgggcc gacgatgacg gcaaggtcca tcgctcgttc ttcatcaacg gcggcggtgc
     3481 ccgcgtcacc ggctccggca agctcgcgag cgagaaccgc gcggtcgccg gcttccaggc
     3541 gatcgcgctg cgtgcgtcga tgaagctggt gctgcgccag ggcccgcgtg aaggcatcga
     3601 gctgcgcgcc gacgacaaca tcctgtcgtt gatcgagacg gtggtggtcg agcgcggcgg
     3661 cgtgccgacg ctggagatcg ccgccaagaa gggcacgaac ttctcgaccg gcaacccgat
     3721 cgtcgcgacc atcgacctgg tgacgctgcg cgcgctgtcg atcagcggtt cgggcgacgt
     3781 ggtcggcgat ggattgaaga cgcccggtct gagcgtggcg atcagcggtt cgggcaacgt
     3841 caggctgaag cagctcgtcg ccgacgaggt ctcggccagg gtctcgggca gcggcgacat
     3901 cgagttcagc ggccgcacgg ccaagctcgg cgtctcgatc tcgggcagcg gcgacgtgaa
     3961 gacgcgcggc ctcgaagccg acgatgtgag cgtcagcgtt gctggcagcg gcgatgccga
     4021 cgtgacggca cgcaggacgc tcacggtctc gatcgccggc gtgggcaacg tcaagtacag
     4081 cggcgacgcc accgtgaagt cgtcgatcgc gggccgcggt aacgtcacca aacagtgagg
     4141 ttgattcgct cataggacaa tggtccgaat gagcgaagaa gtcctgaatc gccgcccggt
     4201 ctggcgcgag cccgaattcg cgcgcggcgt gcgcgacatg gtcggggttt cgctcggcat
     4261 cgcggcctgg gggctggtca ccggcgtggc gatggccaag agcgggctgc cgctgccgct
     4321 gtgcatcctg atgtcgctgc tcgtgttcgc cggcagcgcg cagctcgcgg cgctgccgat
     4381 gctggtctcg ggcgcgccgg tctggatgag ctgggcgatc gcgctgtgcg tgaacctgcg
     4441 cttcctgatc ttcagcgcgc agtggcggcc gtattggtcg cacctgtcac gcgggcagcg
     4501 catgctgcgc ggctacttca ccggcgatct gaactatgtg atcttcatga cgcgctttcc
     4561 cgagccgcgc ccggcgccgg cgcaggagcc gtacttctgg ggcggcgtcg cgaccaactg
     4621 gtcggcgtgg cagattccgt cgctcgccgg catgctgctg gccgacgcga tcccgaccga
     4681 atggggcttc ggtttcgccg gcatcctcgc gctgctcggg ctgatgtact cgctgatgca
     4741 tgaccgcgcc agctgggtcg cggccggtgt cgcgagcgcc gctgcggtgg cggcggcggc
     4801 gctgccgctg aagctgaacg tcgtcgtcgc gatcgccgcg gcgatcgcgg tcgggctgct
     4861 gatggaccac gcgacgccag tcgaggccag gccgatggag gacaacacat gagcacctgg
     4921 gagaccgtga tcgcgatcct ggggctggcg gtcatcaccg tcggcacgcg cggtt
//
LOCUS       DASRDG010000636         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_329541, whole genome shotgun sequence.
ACCESSION   DASRDG010000636 DASRDG010000000
VERSION     DASRDG010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329541"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFA35_10690"
     CDS             complement(<1..400)
                     /locus_tag="VFA35_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HZT56685.1"
                     /translation="MSSTYAALHLRDPQPMLGDALRAWGGEQDLWVFGYASLIWRPEF
                     EADEARTAVVHGWHRALEMRSRVNRGTPECPGLVFALVSGGSCRGMVYRIERARAAAE
                     LERLWLREMPTGVYDPKWLACRTVHGRVKAL"
     gene            442..921
                     /locus_tag="VFA35_10695"
     CDS             442..921
                     /locus_tag="VFA35_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZT56686.1"
                     /translation="MSDENDESRNYALLVLAGLVALVIAGVLALAVHKGLGGSAKPAG
                     TANGAATAIIVGEPQGRIYFELGSDALPADASAILVTVADAARANAGKVVLISGFHDA
                     SGDAATNAELAKKRAQAVRHALEANGVAPERLVLDKPSMTTGGADAREARRVELRLR"
     gene            1058..>2505
                     /gene="edd"
                     /locus_tag="VFA35_10700"
     CDS             1058..>2505
                     /gene="edd"
                     /locus_tag="VFA35_10700"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HZT56687.1"
                     /translation="MKPAVLEVTERIRARSAATRAAYLARVDAAIARPRGADRMGCAN
                     VAHAFAALPGRDKLRIVAERAPNLAVVTSYNDMLSAHQPYEAFPALIRDEAHKLGATV
                     QVAGGVPAMCDGVTQGLPGMELSLFSRDTIAMGTAIALTHDVFDGALLLGVCDKIVPG
                     LLIGALHFGHLPCVFVPAGPMSTGLSNTAKSKVREQFAQGLVGREELLQAESAAYHGA
                     GTCTFYGTANSNQMLLEAMGLHVPGAAFIHPHTEMREALTREAVRKLLGIVKREHFTP
                     IGQLVDERVIVNAMVALLATGGSTNHLIHWVAVARSAGILIDWTDFAELSHVTPLLAR
                     VYPNGTADVNQFQAAGGPGFVIRELIDSGTMHGDVGTVVPGGLREYGKVPTRAGERLT
                     WADLPAAAIDAAIVRTAAAPFGAEGGLKLLTGNLGRAVIKVSAVPEDRHVVEAPAIVF
                     DTQEELLAAFKAGALERDFVAVVRFQGPAACG"
BASE COUNT          380 a          874 c          907 g          344 t
ORIGIN      
        1 cgagcgcctt cacgcggccg tgcacggtgc ggcaggccag ccatttgggg tcgtacacgc
       61 cggtgggcat ctcgcgcagc cacaggcgct cgagttcggc cgcggcccgg gcgcgctcga
      121 tgcggtagac catgccccgg cacgatccgc cggacaccag cgcgaacacg aggccggggc
      181 attcgggcgt gccgcggttc acgcgcgagc gcatctcgag cgcgcgatgc cagccgtgga
      241 cgacggcagt gcgcgcttcg tcggcctcga actcggggcg ccagatcagc gaggcgtagc
      301 cgaacaccca cagatcctgc tcgccgcccc aggcccgcag cgcgtcgccg agcatcggct
      361 gcgggtcgcg caggtgcagg gcggcgtagg ttgaactcat gccgccactc tagaatggaa
      421 atcttcttgg aggagacccg aatgagtgat gaaaacgacg agagccggaa ttacgcactg
      481 ttggtgctgg cgggcctggt ggcgctggtg atcgcgggcg tgctcgcgct ggcggttcac
      541 aaggggctgg gcgggtcggc gaagcctgcg ggcaccgcga acggggcggc gaccgcgatc
      601 atcgtcggcg agccgcaggg ccgcatctac ttcgagctcg gctcggacgc actgccggcc
      661 gatgccagcg cgatcctcgt gacggtggct gacgccgcac gcgcgaacgc cggcaaggtc
      721 gtgctgatct cgggcttcca cgacgccagc ggcgatgccg cgaccaacgc cgaactcgcg
      781 aagaagcgcg cccaggcggt gcgccacgcg ctcgaagcca acggcgtcgc gcccgagcgc
      841 ctggtgctgg acaagccgag catgaccacc ggcggtgccg acgcgcgcga agcccgccgc
      901 gtcgagttgc gcctgcgctg acggggacaa gggcgcaggc ggcgccggcc atggcacttg
      961 cggccgtccc aggcgggcgc gttgccgaat ggaatgattt gacgatgtaa ccaggttacg
     1021 tgaaaatcac cggcatggtt accaccagga gtctgccgtg aagccagccg ttctcgaagt
     1081 caccgaacgc atccgcgcgc gcagcgcggc gacacgggcc gcgtacctcg cgcgcgtcga
     1141 cgcggcgatc gcgcgcccgc gcggcgccga ccgcatgggc tgcgcgaacg tcgcgcatgc
     1201 gttcgcggcg ctgccggggc gcgacaagct gcgcatcgtc gccgagcgcg cgccgaatct
     1261 ggcggtcgtg acctcgtaca acgacatgct ctcggcgcac cagccctacg aagcctttcc
     1321 ggcgctgatc cgcgacgagg cgcacaagct cggcgccacg gtgcaggtcg ccggcggtgt
     1381 gcccgcgatg tgcgacggcg tcacgcaggg cctgcccggc atggagctga gcctgttctc
     1441 gcgcgacacg atcgcgatgg gcaccgcgat cgcgctgacc cacgacgtgt tcgacggcgc
     1501 gttgctgctc ggcgtgtgcg acaagatcgt gccggggctg ctgatcggtg cgctgcactt
     1561 cgggcacctg ccgtgcgtgt tcgtgccggc cgggccgatg agcaccgggc tgtcgaacac
     1621 cgcgaagtcg aaggtgcgcg agcagttcgc gcagggcctg gtcggccgcg aagaactgct
     1681 gcaggccgaa tcggcggcct accacggcgc cggcacctgc accttctacg gcaccgcgaa
     1741 cagcaaccag atgctgctcg aggcgatggg gctgcacgtg ccgggcgccg ccttcatcca
     1801 cccgcacacc gagatgcgcg aggcgttgac gcgcgaggcg gtgcgcaagc tgctcggcat
     1861 cgtgaagcgc gaacatttca cgccgatcgg ccagctcgtc gacgagcgcg tcatcgtcaa
     1921 cgcgatggtc gcgctgctcg cgaccggcgg ctcgacgaat cacctgatcc actgggtcgc
     1981 ggtcgcgcgc tcggccggca tcctgatcga ctggaccgac ttcgccgagc tctcgcacgt
     2041 gacgccgctg ctggcgcgcg tctacccgaa cggcactgcc gacgtgaacc agttccaggc
     2101 cgccggcggg ccgggcttcg tgatccgcga gctgatcgac agcggcacga tgcatggcga
     2161 cgtcggcacc gtcgtgcccg gcggcctgcg cgagtacggc aaggtgccga cgcgggccgg
     2221 cgagcggctg acctgggccg acctgccggc cgcggcgatc gacgccgcca tcgtgcgcac
     2281 cgccgcggcg ccgttcggcg ccgagggcgg cctgaagctg ctgaccggca acctcggccg
     2341 cgccgtcatc aaggtctcgg cggtgccgga agaccgccat gtcgtcgagg ccccggcgat
     2401 cgtcttcgac acgcaggaag aactgctcgc cgcgttcaag gccggcgcgc tggagcgcga
     2461 tttcgtcgcg gtcgtgcgct tccaggggcc ggccgcctgc ggcat
//
LOCUS       DASRDG010000637         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1359304, whole genome shotgun sequence.
ACCESSION   DASRDG010000637 DASRDG010000000
VERSION     DASRDG010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359304"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..717
                     /locus_tag="VFA35_10705"
     CDS             61..717
                     /locus_tag="VFA35_10705"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZT56688.1"
                     /translation="MVGAPHTEAAIHAAGIAFDGYPSFVARCCGLPADEAMLMLCFAH
                     AGREFRIAHVTLHASVREALAQITGPRIIGTLRATENALKVLGIERPRIAVSGVNPHA
                     GEGGAFGREELEVVTPALEQLRRAGMAVEGPFGADSLLQRAEYDAYVVMLHDQGHVPA
                     KMLAPLRAAALTIGTPVLFSSVGHGSALDIAGKGIADASAMREAITRLVSRRRRTPAT
                     "
     gene            959..1228
                     /locus_tag="VFA35_10710"
     CDS             959..1228
                     /locus_tag="VFA35_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56689.1"
                     /translation="MYVALMAALPLVTASMSQMRYAAQASAATTQPTFEQLDRNRDGY
                     VDRREAEWLPGLDAIFDAADRRADGRLDKVEYAKALAMLDPAGRP"
     gene            1225..2019
                     /locus_tag="VFA35_10715"
     CDS             1225..2019
                     /locus_tag="VFA35_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56690.1"
                     /translation="MNLSFGLVKTAADEAADALLAREARWLRELEQVRELEGQVPRVL
                     EEGTSPAGRRYLVISMWPARGETRAFTADHARFLATLGRARFRSLEFDACGTSQWMQE
                     SLAQAHEFAPQRSLELLQDAYHDCETALLYWTGPFVLSQGDFAPWNIRNLGSQLFVTD
                     WGEAKSDASPLDDVLHYLMIQRALNHRPVTVPVLRQAMSRAAEFAVQAYPEWNWRAPV
                     IGALTLVYLLGVVLHRSLARRRIDRTDRVVGAYWKLLEKRSAWMPK"
BASE COUNT          354 a          768 c          752 g          324 t
ORIGIN      
        1 tcggcgctgg aagcggccgc cgcagcgctc gagggcgcgc gcgcgggcaa gtacgaagcg
       61 gtggtcggtg cgccgcacac cgaggcggcg atacacgccg ccggcatcgc gttcgatggc
      121 tatccgtcgt tcgtcgcccg ctgctgcggc ctgcccgccg atgaagcgat gctgatgctc
      181 tgcttcgcgc atgcggggcg cgagttccgg atcgcgcacg tcacgctgca tgcgagcgtg
      241 cgcgaagcac tggcgcagat caccggaccg cgcatcatcg gcacgctgcg cgcgaccgag
      301 aatgcgctga aggtgctggg catcgagcgg ccgcgcatcg cggtgagcgg ggtgaacccg
      361 catgccggag agggcggcgc ctttggccgc gaggaactcg aggtggtgac gcccgcgctc
      421 gaacagctca gacgcgccgg catggcggtc gaaggaccgt tcggtgccga cagccttctg
      481 cagcgtgctg agtacgacgc ctatgtcgtg atgctgcacg accagggcca tgtgccagcg
      541 aagatgctgg cgccgctgcg cgctgccgcg ctcaccatcg gcacgccggt acttttttct
      601 tccgtcggcc acggctccgc gctcgatatc gccggcaaag gcatcgccga cgcgagcgcc
      661 atgcgagaag caattacacg tctagtcagt cgtcgacgtc gcacgcctgc gacctgaaat
      721 cccgcacgca aacaaggtag tagcgattcg ctggcagcgc gcagtccgcg cgcggcgacg
      781 cttcgcgcgc tccgcgcgaa cggcgatttg ccactaactc gttgccgcag cgcgcttttc
      841 gcgggctggt acggcgcttg cacagagagc tcccgcaagc cccgaccaac gacaccaacg
      901 ctcgatgaat acacggctgc gccagcagcg cacgaatttc ctctttttcc ttggcgccat
      961 gtacgtcgct ctgatggcgg cgctgccgct ggtgacggct tcgatgtcgc agatgcgcta
     1021 tgcggcgcag gcgagcgccg cgacgacgca gccgaccttc gagcagctcg atcgcaaccg
     1081 cgacggctat gtcgatcggc gtgaagccga gtggctgccg ggactcgacg cgatcttcga
     1141 tgccgctgac cgacgcgccg atggccgtct cgacaaggtc gagtacgcga aagcgctggc
     1201 gatgctcgac ccagcaggca ggccttgaac ctgtcctttg gcctggtgaa gacggcggcc
     1261 gacgaggcgg ccgacgcgct cctcgcgcgc gaggcgcgct ggctgcgcga gctcgagcag
     1321 gtgcgcgagc tcgaggggca ggtgccgcgc gtgctcgaag aaggcacgag cccggccggc
     1381 cggcgctatc tcgtgatcag catgtggccg gcgcgcggcg agacccgtgc cttcaccgcc
     1441 gaccatgcgc gctttctcgc cacgctcggg cgcgcgcgct tccgctcgct cgagttcgac
     1501 gcctgcggca ccagccagtg gatgcaggag tcgctcgccc aggcgcacga gttcgcgccg
     1561 cagcgctcgc tcgagctgct gcaggacgct taccacgact gcgagacggc gctcctctac
     1621 tggaccggcc cgttcgtgct gtcgcagggc gacttcgcgc cttggaacat ccgcaacctc
     1681 ggctcgcagc tcttcgtcac cgactggggc gaggcgaaga gcgacgcgag cccgctcgac
     1741 gacgtgctgc attacctgat gatccagcgc gcgctcaacc accggccggt caccgtgccg
     1801 gtgctgcgcc aggcgatgag ccgcgccgcc gagttcgccg tgcaggccta cccggaatgg
     1861 aactggcgcg cgccggtgat cggcgcgctg acgctcgtct acctgctggg cgtggtgctg
     1921 catcgctcgc tcgcccggcg acgcatcgac cgcaccgacc gcgtggtcgg cgcgtactgg
     1981 aagttgctcg agaaaagatc ggcatggatg ccgaaataac ccgaaggaag gaaacgtcat
     2041 gagcgccgcc gccaccgctg ccggctatct gtacgcctcc ggccgcctgc gcggacgcgc
     2101 gcagcgcgag caggaggaag gccacaacga acaccaccac gagcacggcg cacccgaagc
     2161 gcgccccgag cagtccaagc cgcgcggcga gcgcccgc
//
LOCUS       DASRDG010000638         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1251002, whole genome shotgun sequence.
ACCESSION   DASRDG010000638 DASRDG010000000
VERSION     DASRDG010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251002"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VFA35_10720"
     CDS             complement(<1..1067)
                     /locus_tag="VFA35_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZT56691.1"
                     /translation="MPPTDASSPATIDIESLGLDQGGLLLLRRALAEIAPGAELRVRG
                     RAPGWTVHLAAWCRQHGHGLRFDERPDGSCELWVARASHAEGRWHQAMHTGRAWLGDG
                     EWPAAEAAPAWGLAARGATVEAGSPAFAFALARRDELWAATAGALYRQGAAAQWDPAL
                     AIDWGAAFTLAPAIEAAVVQVMTYLIENENAALLVPARHLGQVHPHYREVMQLLALQC
                     ADEARHVEVFTRRATLRGCEPALSSAGGQASLRTLFEASDFSQATFLLAVLGEGTFVN
                     LLNFLHAHAPDPVTRQIARLAARDEARHVAFGMAHLEHRLAAQPDYRASLRNAIEQRY
                     DELAATTGLNEEVFDALVLLAA"
     gene            complement(1024..1896)
                     /locus_tag="VFA35_10725"
     CDS             complement(1024..1896)
                     /locus_tag="VFA35_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HZT56692.1"
                     /translation="MAAPMDPNPLVMGAVAYAPKVVTIWEGFKAWFAEQGLAFDYVLF
                     SNYERQVEAQFDGRIQLAWNSPLAWERARRIAAARGLKVEAVAMRDTDCDLRSVFVVR
                     DDRAIATLADLRGRTLGVGAIDSPQATLIPLDHLRQLGLVGGRDFRVRRFDVLGGKHG
                     DHIGGERDAARALLAGEVDACCMIDGNHLAFGLDGTLPAGSTRILATTGAFDHCNFTA
                     SPGAPEAAVRQFTGLLLKMRWDDPQVRPLLELEGLRAWQRGRTSGYALLARAIDDEAF
                     YAADGRILAGDYRY"
     gene            complement(1896..>2815)
                     /locus_tag="VFA35_10730"
     CDS             complement(1896..>2815)
                     /locus_tag="VFA35_10730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZT56693.1"
                     /translation="IAQACPSTAMVLCMHYCATAVIEALGPDALRREIAAGRHLSTLA
                     FSEAESRSHFWAPLSSARAAGAQVLLDARKSWVTSAFEADSYVWSSKPLAAEGASTLW
                     LVDAKLPGLAQPGRFDGLGLRGNASTPITAKEVAVAADARLGDDGKGFDTMMGVVLPW
                     FSVLSAACSVGLMEGAITRAAGHVAQTRLQHLGSSLADLPTIRAYLARGRIQTDMAGA
                     LLHDTITAIGTGRPDTMLRVLEVKAGAAEAALAVTDIAMRVCGGAAFRKEAGIERLFR
                     DARASSVMGPTSDVLYDFIGKAVTGLPLF"
BASE COUNT          397 a         1033 c         1019 g          366 t
ORIGIN      
        1 gccgccagca gcacgagcgc gtcgaagacc tcttcgttca gcccggtcgt cgcggcgagt
       61 tcgtcgtagc gttgctcgat ggcattgcgc aggctggctc ggtagtccgg ctgcgcggcc
      121 agccggtgct ccagatgggc catgccgaag gcgacgtggc gcgcctcgtc gcgcgccgcg
      181 agccgcgcga tctggcgtgt cacggggtcg ggcgcgtgcg cgtgcaggaa gttgagcagg
      241 ttcacgaacg tgccctcgcc gagcaccgcc agcaggaagg tcgcctgcga aaaatcgctg
      301 gcctcgaaca gcgtgcgcaa cgaggcctgg ccacccgcgc tcgacagcgc cggctcgcag
      361 ccgcgcaagg tggcgcggcg cgtgaacacc tcgacatgac gcgcctcgtc ggcgcattgc
      421 agcgccagca actgcatcac ctcgcggtag tgcggatgga cctggccgag gtggcgcgcc
      481 ggcaccagca gcgccgcgtt ctcgttctcg atcaggtagg tcatcacctg cacgaccgcg
      541 gcctcgatgg ccggcgcgag cgtgaaggca gcgccccagt cgatcgccag cgccgggtcc
      601 cattgcgcgg cggcgccctg gcggtacagg gcgcccgcgg tggcggccca aagctcgtcg
      661 cgccgcgcca gcgcgaaggc gaacgccggg ctgccggcct cgacggtcgc gccgcgcgcc
      721 gccagccccc acgcgggcgc ggcctcggca gcgggccact cgccgtcgcc gagccaagcg
      781 cgcccggtgt gcatcgcctg gtgccagcgc ccttcggcat gcgacgcgcg cgccacccac
      841 agctcgcagc tgccgtcggg acgttcgtcg aagcgcaggc cgtggccgtg ctgccggcac
      901 cacgcagcca ggtgcaccgt ccagcccggc gcgcggccgc gcacccgcag ttccgcgccg
      961 ggcgcgatct cggccagcgc gcgccgcaac agcagcagcc cgccttgatc gagcccgagc
     1021 gactcaatat cgatagtcgc cggcgaggat gcgtccgtcg gcggcataga aggcttcgtc
     1081 gtcgatcgcg cgggcgagca gcgcgtagcc gctggtgcgc ccgcgctgcc aggcccgcag
     1141 gccttccagc tcgagcagcg gccgcacctg cgggtcgtcc cagcgcatct tcagcagcag
     1201 gccggtgaac tgtcgcaccg ctgcctcggg tgcgcccggg ctggccgtga agttgcaatg
     1261 gtcgaacgcg ccggtcgtcg ccagaatgcg cgtgctgccc gccggcagcg tgccgtcgag
     1321 cccgaacgcc aggtggttgc cgtcgatcat gcagcaggcg tcgacctcgc cggccagcag
     1381 cgcccgcgcg gcgtcacgct cgccgccgat gtggtcgccg tgcttgccgc cgagcacgtc
     1441 gaagcggcgc acccggaagt cgcgcccacc gacgagaccg agctgccgca ggtggtcgag
     1501 cgggatcagc gtggcctgcg gcgagtcgat cgcgccgacg ccgagcgtgc ggccgcgcag
     1561 atcggccagc gtggcgatgg cgcggtcgtc gcgcacgacg aacaccgagc gcaggtcgca
     1621 gtcggtgtcg cgcatcgcga cggcctcgac cttcaggccg cgcgccgcgg cgatgcggcg
     1681 cgcacgctcc caggccagcg gcgagttcca cgcgagctgg atgcggccgt cgaactgggc
     1741 ttcgacctgg cgctcgtagt tcgagaacag aacatagtcg aaggccaggc cctgctcggc
     1801 gaaccaggcc ttgaagccct cccagatcgt gacgaccttg ggtgcgtagg cgaccgcacc
     1861 catcaccagc gggttcgggt ccattggcgc ggccatcaga acagcggcag gccggtgacc
     1921 gccttgccga tgaagtcgta gagcacatcg gacgtcggcc ccatcacgct ggacgcacgc
     1981 gcgtcgcgga acaggcgctc gatgcccgcc tccttgcgga aagccgcgcc gccgcagacg
     2041 cgcatcgcga tgtcggtgac cgccagcgcg gcttcggcgg cgccagcctt gacctcgagc
     2101 acgcgcagca tcgtgtcggg gcggccggtg ccgatcgcgg tgatggtgtc gtgcagcagc
     2161 gcgccggcca tgtcggtctg gatgcggccg cgcgccaggt aggcgcggat cgtcggcagg
     2221 tcggcgagcg aactgcccag gtgctgcagc ctggtctgcg cgacgtggcc tgcggcgcgc
     2281 gtgatcgccc cttccatcag gcccaccgag caggccgcgc tgagcaccga gaaccacggc
     2341 aggaccacgc ccatcatcgt gtcgaagccc ttgccgtcgt cgccgagccg cgcgtcggcc
     2401 gcgaccgcga cctccttggc ggtgatcggc gtcgaagcgt tgccgcgcag gcccaggccg
     2461 tcgaacctgc cgggctgggc gagccccggc agcttcgcgt cgacgagcca gagcgtgctc
     2521 gcgccctcgg cggccagcgg cttgctcgac cacacatagc tgtcggcctc gaacgcgctc
     2581 gtgacccagc tcttgcgcgc atcgagcagc acctgcgcgc cggccgcgcg cgcgctgctg
     2641 aggggtgccc agaaatggct gcgcgactcg gcctcggaga acgcgagcgt gctcaggtgg
     2701 cggccggcgg cgatctcgcg gcgcagcgcg tcgggcccga gcgcctcgat cacggcggtc
     2761 gcgcagtagt gcatgcacag caccatcgcg gtcgacgggc aggcctgcgc gatga
//
LOCUS       DASRDG010000639         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1131399, whole genome shotgun sequence.
ACCESSION   DASRDG010000639 DASRDG010000000
VERSION     DASRDG010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131399"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /gene="cydD"
                     /locus_tag="VFA35_10735"
     CDS             complement(<1..1630)
                     /gene="cydD"
                     /locus_tag="VFA35_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HZT56694.1"
                     /translation="MPTALLTPPRLASMQQAGAVIQLFAALLWLPQAGLMAWAVQRLA
                     EGGGVSAVLPAVAGVAVLGLLRAACDAWGLRLAFSAARRELTALRAGVAASLATGSTP
                     GAQQTSSGTAASLIAEQAEAVLPWRVRFAPVRLRVTWVPIVLLLAVLWWSWAAALILL
                     VAAPLIPLFMAIVGWRAQAASKAQMVELGEMNGFLLDRLRGLPTLRALSAVQFTAGRV
                     LAAAQDLRRRTMAVLRIAFLSSAVLELFAALGVAMVAAYVGFHLLGQFRFGTWGHRLS
                     LGEGFFILLLAPAFFEPLRDLAAVWHDRAAGEAALGQLERLAPPPADPLDARMSVITP
                     ALPPGPLELRVRGLGFAHRERAPVFEGFALHVQPGEHVALVAASGGGKSTLLALLAGL
                     HVPQAGEVRIGSVPMNASSAEALRRRIGWMDQRPHVFAGSLRANVALGRPGVGAADID
                     AALRLAGLGELAAARPAASLGEGGQGLSGGELVRLALARLAVQHDAGLLLIDEPTAHL
                     DGETAAQVTDALLQLARGRTLIVATHDPQLAARMH"
     gene            complement(1823..>2938)
                     /locus_tag="VFA35_10740"
     CDS             complement(1823..>2938)
                     /locus_tag="VFA35_10740"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZT56695.1"
                     /translation="SYSAQAHWHHFRYRIGELIGLDRLSRQVHIAPFVDSDGIQVTPQ
                     RSFGYDTLVIAIGSQSNDFGTPGVADHAMRLETTADAQRFNRRLVNSCIRANAQSGPL
                     EPHQLRVAIIGAGATGVELSAELHRTTREVISFGLDHIDPDKDLQIHLIEAADRVLPG
                     LPPGTSAATQSLLEELRVQVHTKAKVEAVLPKGVRLADGREIPAELVVWAAGVKAADV
                     LKDLAGLESNRINQLVVRPTLQTTRDDDIFAIGDCAACAWPEANGGKGGFVPPRAQAA
                     HQQASLVAAQIKRRLAGKPLRDYRYRDFGSLVSLSDYSTVGNMAGNMVIQGWFARMMY
                     LSLYKMHELALHGFWKVSLETFARMLTRRTEPHVKLH"
BASE COUNT          448 a         1062 c         1041 g          387 t
ORIGIN      
        1 gatgcatccg cgcggccagc tgcgggtcgt gcgtggcgac gatcagcgtg cggccgcgcg
       61 cgagctgcag cagcgcgtcc gtcacctgcg ccgccgtttc gccgtcgagg tgggcggtcg
      121 gctcgtcgat cagcagcaag cccgcgtcgt gctgcaccgc caggcgcgcc agcgccagcc
      181 gcaccaattc gccgccggag aggccttggc caccctcacc gagcgacgcc gccggtcgcg
      241 ccgccgccag ttcgcccagg ccggccaggc gcagcgccgc gtcgatgtcg gccgcgccga
      301 cgccggggcg cccgagcgcc acgttggcgc gcagcgagcc cgcgaacaca tggggccgct
      361 ggtccatcca gccgatgcgc cggcgcagcg cctcggcgct gctggcgttc atcggcaccg
      421 atccgatgcg cacctcgccg gcctgcggca cgtgcagccc ggccagcagc gccagcagcg
      481 tgctcttgcc gccgccgctg gccgcgacca gcgcgacgtg ctcgccgggc tgcacatgca
      541 gcgcgaaacc ctcgaagacc ggcgcgcgct cccggtgcgc gaagccgagg ccacgcaccc
      601 gcagctccaa cgggcccgga ggcagcgccg gcgtgatcac gctcatgcgc gcgtccagcg
      661 ggtcggcagg cggcggcgcg aggcgctcca gctggcccag cgccgcctcg ccggcggcgc
      721 ggtcgtgcca gaccgccgcc agatcgcgca acggctcgaa gaaggccggc gccagcagca
      781 ggatgaagaa gccctcgccc aggctgagcc tgtgccccca cgtgccgaag cggaactgcc
      841 cgagcagatg gaagccgacg tatgccgcca ccatcgccac cccgagcgcg gcaaacagct
      901 ccagcacggc cgaggacagg aaggcgatgc gcagcaccgc catcgtgcgc cgccgcaggt
      961 cttgagcggc ggcgagcacg cggccggcgg tgaattgcac ggcactcagc gcgcgcagcg
     1021 tcggcaggcc gcgcagacga tcgagcagga agccgttcat ctcgcccagt tccaccatct
     1081 gcgccttgct ggccgcctgg gcgcgccagc cgacgatggc catgaacagt ggaatgagcg
     1141 gcgccgcgac gagcaggatc agcgccgccg cccacgacca ccacagcacg gccagcagca
     1201 gcacgatcgg cacccaggtc acgcgcagcc gcaccggtgc gaagcgcacg cgccagggca
     1261 gcacggcctc ggcctgctcg gcgatgaggc tggcggccgt gccggacgat gtctgctgcg
     1321 cgcccggtgt cgaccctgtg gccagggacg ccgccacgcc ggcgcgcaat gccgtcagtt
     1381 cgcgccgggc ggccgagaag gccaggcgca ggccccaggc atcgcacgcg gcccgcagca
     1441 ggccgagcac ggcgacgccc gccaccgccg gcagcacggc cgacacaccg ccgccctcgg
     1501 ccagccgctg cacggcccac gccatgagcc ccgcctgtgg cagccacagc agcgccgcaa
     1561 aaagctggat cacagcacca gcttgctgca tggaagcaag cctgggcggc gtcaggaggg
     1621 cggtcggcat tttgtggcta cagaattctc agtagttctt gaaaattccg ctgttatacg
     1681 ccgcaagagt cggggcgaag atgcgggccc ggtcagccat gggttcggcg ggatagaacg
     1741 ggtcctcgac gaccggtagg gcgcgccggt gtcgcgcgcc caggtcacgc atccagggcg
     1801 ggtcgttgcc gccggcccgc gatcagtgca gcttgacgtg cggttcggtg cggcgtgtga
     1861 gcatccgcgc gaacgtctcg agcgacacct tccagaagcc gtgcagcgcc agttcgtgca
     1921 tcttgtacag cgacaggtac atcatgcggg cgaaccagcc ctggatcacc atgttgccgg
     1981 ccatgttgcc gacggtgctg tagtcgctga gcgaaaccag cgacccgaag tcgcgatagc
     2041 ggtagtcgcg cagcggcttg ccggccagcc ggcgcttgat ctgcgcggcg acaagcgagg
     2101 cctgctggtg cgcggcctgc gcgcgcggcg gcacgaagcc gcccttgccg ccgttcgctt
     2161 cgggccaggc gcaagcggcg cagtcgccga tcgcgaagat gtcgtcgtcg cgggtggtct
     2221 gcaaggtcgg ccgcacgacc agttgattga tccggttgct ctccaggccg gcgagatcct
     2281 tgagcacgtc cgcggccttg acgcccgccg cccagaccac cagctccgcg gggatctcgc
     2341 gcccgtcggc gaggcgcacg cctttcggca gcacggcctc gaccttggcc ttggtgtgca
     2401 cctggacgcg cagttcctcg agcaggctct gggtcgcggc cgaggtgccc ggcggcaacc
     2461 cgggcagcac gcggtcggct gcctcgatga ggtgaatttg caggtccttg tcgggatcga
     2521 tgtggtcgag tccgaacgag atgacctcgc gcgtcgttcg gtgcagttcc gccgacagtt
     2581 cgacgccggt cgccccggcg ccgatgatcg ccacccgcaa ctggtgcggc tcgagcggcc
     2641 cgctttgcgc gttggcgcga atgcagctgt tgacgagacg gcggttgaag cgttgcgcgt
     2701 cggcggtggt ctcgagccgc atcgcatggt cggcgacgcc cggcgtgccg aaatcgttgc
     2761 tctggctgcc gatcgcgatc accagcgtgt cgtagccgaa actgcgctgc ggcgtgacct
     2821 ggatgccgtc gctgtcgacg aacggcgcga tgtgcacctg gcggctcagc cgatcgaggc
     2881 cgatcagctc gccgatccga tagcggaagt ggtgccaatg cgcctgcgcg ctgtacga
//
LOCUS       DASRDG010000640         5079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1482060, whole genome shotgun sequence.
ACCESSION   DASRDG010000640 DASRDG010000000
VERSION     DASRDG010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482060"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..370
                     /locus_tag="VFA35_10745"
     CDS             68..370
                     /locus_tag="VFA35_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56696.1"
                     /translation="MTNEAFARVKIDSLLAAQGWDTLDTNAVRFEVQLPDGTRADYVL
                     CDRHGRALAVIEAKRYSVSPGDAARGCATAVRRASRGDRIDAAATGRGPREGSSHL"
     gene            683..1852
                     /locus_tag="VFA35_10750"
     CDS             683..1852
                     /locus_tag="VFA35_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZT56697.1"
                     /translation="MLLSGCGKPQGAEPGAPGHGPGGGAPPAMPVAVLAASTRSVPVL
                     IDAVGRAEGSKEVEVRARVTGLIEQQRYREGDRVKAGAKLFGIERAPFEIALAQARAG
                     QAQEQARLDQARREAKRLAPLAEQRAISQREYDDAASTLSLAEAAAAAARAQVAQAQL
                     NLSYTSVVAPITGVTGRAEKSQGSLVSPTDGLLTRVTQTDPIWVRFSFSEGELARLRS
                     GKAGAVRLLSADGQPVGAPGKLNFEGSTVDPQLGTVQLRAEFPNPDLALLPGQFVRAQ
                     VHAGEERAFVVPQAAMTTGERGKAVWVVKDGKALPAPVEVGAWVGDGWAVRKGLTEGD
                     PVIVDNLLKLRPGAPVAPHAASAPANGGAASAPAASAPAASAAAPASAAASTPSR"
     gene            1863..5033
                     /locus_tag="VFA35_10755"
     CDS             1863..5033
                     /locus_tag="VFA35_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HZT56698.1"
                     /translation="MFSRFFIERPVFAAVLSIIILLAGAVSLKILPIAQYPEIAPPTV
                     SVIANYPGASAQTLAATVAAPIEEQLSGVENLQYYSSTSASNGQTEITVTFEVGTDID
                     KATFDVNNRVQLATPRLPDEVRRQGVTVAKRSNNFLLVMALNSPKSSYDQIYISNYAT
                     QNVVDNIKRVPGVADVFIFGARDYSMRLWLQPEKMARLGITTGDIRSAVNAQNAQFAA
                     GKIGAEPSPKGQQIEYTVTTRGRLLEAEQFGDIVLRAGGPTGVLRLKDVARVELGAQS
                     YDATNSINGQPAIAIAVFLASGANALQVGDAVKARMAALKSDRFPPDLDYSIPYDTTR
                     FVKASIDEVMFTMLDAAVIVLLVVFVFLQTWRATLIPMLAVPVSLIGTFAGLWLFGFS
                     INTLTLFAMVLAIGIVVDDAIVVLENVERLIRTEKMTPMAAAIEAMHEVQGAVIGIVL
                     ALCAVFIPVAFLGGIAGQLYRQFAVTVTVAVVLSGIVALTLTPALCAVLLKPGDHDGD
                     HWQARLFRPFNSALAWVTEHFVRGVGFALRNRVVALLGFAAIIAVAALLLWRVPSSFV
                     PPEDQGYIVAIAVLPDGATLERTTRTTEQLRHMMDGNKAVENFFSVNGFDFIGGGSKA
                     NAATMFLPMVPWDKRQQSSEQLTAEMSGKGFALNDGIAFAFNPPAIQGLSQAGGFEVF
                     VQARSDPDPQRLAQVTGAFMQALQKRPEMGMVNTFFRPTVPQLQVEVDREKAMALGVN
                     VDEVFTALQAQMGSLYVNDFNKAGRTYRVTMQADAQFRAKPEDLGAIYVRSTTSGQMI
                     PLKALIRVDNVIGPEQLQRYNGFVAARILGNAKEGYSSGQAIRAVEETAKTALPPGYD
                     MAWTGQAYQEKRTGNASVFAFGFAIVMVYLILSALYERWGVPFAVVLAVPFALTGALL
                     FVWLRHMENDIYFQIGLVVLIGLAAKNAILIAEFAMQGMEAGKSAAEAARDAARLRFR
                     PIVMTSLAFVFGVVPLVTATGAGAAARQSMGTGVFGGMIVATFVAPIFVPLFFTLLAR
                     KPRPRHEHHGEAQALQAPGGEAGGSS"
BASE COUNT          762 a         1784 c         1778 g          755 t
ORIGIN      
        1 aagccgagcc ggttgttcgt gccgatgcac ctggtagcgc agttgctggg ttagcctgcc
       61 cgatgccatg acgaacgaag cctttgcccg ggtcaagatt gactcgctgc tcgcagcgca
      121 gggctgggac acgctcgaca ccaacgccgt ccgcttcgag gtccaactgc cagacggcac
      181 gcgcgctgac tatgtgctgt gcgaccgcca cggccgcgca ctggccgtga tcgaagccaa
      241 gcgctactcg gtcagccctg gcgacgcggc ccgaggatgc gcaactgcag ttcgcagagc
      301 gagtcgcggc gatcgaatcg atgctgcggc aacaggtcga gggcctagag aaggcagcag
      361 ccacctttga cgctctgctg tcacgagctt ccggctgagg aagcgctgcc gccatgcctt
      421 cgggcgtcct gcatccttac acactgctgg ggatcgagga ccagatcatc tatcaggcga
      481 tggccaacgt cgttgccgaa cggcgagcaa cgcccgccgc ggttttaaag tacatttttc
      541 cgtcctgcga atcccgcagc aaggctcatt catggcgcag acgatcgcac aaccttcgcc
      601 ccgcaccacc cgtttctctc gcgtcccgtc actcccttcg catcgcgctc gcgcgctcgt
      661 cgcgagcctg ctgggatcgg cgttgctgct gtcgggctgc ggcaaacccc aaggcgccga
      721 acccggcgca ccgggccacg gccccggcgg cggcgcgccg cccgcgatgc cggtcgccgt
      781 gctcgcggcg agcacgcgca gcgtgccggt gctgatcgac gcggtcggcc gcgccgaagg
      841 ctccaaggag gtcgaagtgc gcgcccgcgt gaccgggctg atcgagcagc agcgttaccg
      901 cgaaggcgac cgcgtgaagg ccggcgccaa gctgttcggc atcgagcgcg cgccgttcga
      961 gatcgcgctc gcgcaagcgc gcgccgggca ggcgcaggag caggcccgcc tcgaccaggc
     1021 gcggcgcgaa gccaagcggc tcgcgccgct ggccgagcag cgcgcgatca gccagcgcga
     1081 gtacgacgat gccgcctcga ccctgagcct ggccgaggcc gcggccgccg ccgcgcgcgc
     1141 gcaggtcgcg caggcgcagc tgaacctgtc gtacaccagc gtggtggcgc cgatcaccgg
     1201 cgtcaccggc cgcgccgaga agtcgcaggg cagcctggtc agcccgaccg atggcctgct
     1261 cacgcgcgtg acgcagaccg atccgatctg ggtgcgcttc tcgttctccg aaggcgagct
     1321 ggcgcggctg cgcagcggca aggccggcgc ggtgcgcctg ctgtcggccg acggccagcc
     1381 ggtgggcgcg ccgggcaagc tcaacttcga aggctcgacg gtcgatccgc agctgggcac
     1441 ggtgcagctg cgcgccgagt tcccgaaccc cgacctcgcg ctgctgccgg gccagttcgt
     1501 gcgcgcccag gtgcacgcgg gcgaggagcg cgccttcgtc gtgccgcagg cggcgatgac
     1561 caccggcgag cgcggcaagg cggtctgggt cgtgaaggac ggcaaggcgc tgcccgcgcc
     1621 ggtcgaggtc ggcgcctggg tcggcgacgg ctgggcggtg cgcaagggcc tgaccgaagg
     1681 cgacccggtg atcgtcgaca acctgctgaa gctgcgcccc ggcgcgccgg tggcgccgca
     1741 tgccgcgtcg gcgccggcca acggcggcgc ggcgtcggca cccgcggcgt cggcacctgc
     1801 agcatcggcc gcggcgccgg catcggccgc tgcgtccacg ccctcacgct gaggcccgca
     1861 ccgtgttctc gcgcttcttc atcgaacgcc cggtgttcgc cgcggtgctg tcgatcatca
     1921 tcctgctggc cggcgcggtc tcgctgaaga tcctgccgat cgcgcagtac cccgagatcg
     1981 cgccgcccac cgtcagcgtg atcgcgaact accccggcgc gtcggcgcag acgctcgccg
     2041 cgaccgtggc cgcgccgatc gaggagcagc tctcgggggt cgagaacctg cagtactaca
     2101 gctcgacctc ggcctcgaac ggccagaccg agatcaccgt gaccttcgag gtcggcaccg
     2161 acatcgacaa ggccaccttc gacgtcaaca accgcgtcca gctcgccacg ccgcgcctgc
     2221 ccgacgaggt gcggcgccag ggcgtgacgg tcgcgaagcg ctcgaacaac ttcctgctcg
     2281 tgatggcgct gaattcgccg aagagcagct acgaccagat ctacatctcg aactacgcga
     2341 cccagaacgt cgtcgacaac atcaagcgcg tgcccggcgt cgccgacgtc ttcatcttcg
     2401 gcgcgcgcga ctactcgatg cggctgtggc tgcagcccga gaagatggcg cggctgggca
     2461 tcacgaccgg cgacatccgc agcgcggtga acgcgcagaa cgcgcagttc gcggccggca
     2521 agatcggcgc cgagccctcg ccgaaaggcc agcagatcga gtacaccgtg acgacgcgcg
     2581 gccggctgct cgaggccgag cagttcggcg acatcgtgct gcgtgccggc ggccccaccg
     2641 gcgtgctgcg gctgaaggac gtcgcgcggg tcgagctcgg cgcgcagagc tacgacgcga
     2701 ccaacagcat caacggccag ccggcgatcg cgattgcggt gttcctcgcg tcgggcgcga
     2761 acgcgctgca ggtcggcgat gcggtcaagg cgcgcatggc cgcgctgaag tccgaccgct
     2821 tcccgcccga cctcgactac tcgatcccct acgacaccac gcgcttcgtg aaggcctcga
     2881 tcgacgaggt catgttcacg atgctcgacg ccgcggtcat cgtgctgctg gtcgtgttcg
     2941 tgttcctgca gacctggcgc gccacgctga tcccgatgct ggcggtgccg gtgtcgctga
     3001 tcggcacctt cgccgggctg tggctgttcg gcttctcgat caacacgctg acgctgttcg
     3061 cgatggtgct ggcgatcggc atcgtcgtcg acgacgcgat cgtcgtgctc gagaacgtcg
     3121 agcgcctgat ccgcaccgag aagatgacgc cgatggccgc cgcgatcgag gcgatgcacg
     3181 aggtccaggg cgcggtgatc ggcatcgtgc tggcgctgtg cgcggtgttc atcccggtgg
     3241 cctttctcgg cggcatcgcc gggcagctgt accggcagtt cgcggtcacc gtgacggtcg
     3301 cggtcgtgct ctcgggcatc gtcgcgctga cgctgacgcc ggcgctgtgc gcggtgctgc
     3361 tcaagcccgg cgaccacgac ggcgaccatt ggcaggcgcg cctgttccgg ccgttcaaca
     3421 gcgcgctcgc ctgggtcacc gagcacttcg tgcgcggcgt cggcttcgcg ctgcgcaacc
     3481 gcgtggtcgc gctgctcggc ttcgcggcga tcatcgcggt cgcggcgctg ctgctgtggc
     3541 gcgtgccgag cagcttcgtg ccgcccgaag accagggcta catcgtcgcg atcgcggtgc
     3601 tgcccgacgg cgcgacgctc gagcgcacga cccgcaccac cgagcagctg cgccacatga
     3661 tggacggcaa caaggcggtc gagaacttct tcagcgtcaa cggcttcgac ttcatcggcg
     3721 gcggcagcaa ggcgaacgcg gcgacgatgt tcctgccgat ggtgccgtgg gacaagcgcc
     3781 agcagagctc cgagcagctg accgccgaga tgagcggcaa gggcttcgcg ctgaacgacg
     3841 gcatcgcgtt cgcgttcaac ccgccggcga tccaggggct gagccaggcg ggcggtttcg
     3901 aggtgttcgt tcaggcgcgc agcgaccccg acccgcagcg cctcgcgcag gtcaccggcg
     3961 ccttcatgca ggcgctgcag aagcggcccg aaatgggcat ggtcaacacc ttcttccgcc
     4021 cgaccgtgcc gcagctgcag gtcgaggtcg accgcgagaa ggcgatggcg ctcggcgtca
     4081 acgtcgacga ggtgttcacc gcgctgcagg cgcagatggg ttcgctgtac gtcaacgact
     4141 tcaacaaggc ggggcgcacc taccgcgtga cgatgcaggc cgacgcgcag ttccgcgcca
     4201 agcccgaaga cctgggcgcg atctacgtgc gctcgaccac gagcgggcag atgatcccgc
     4261 tgaaggcgct gatccgggtc gacaacgtga tcggccccga gcagctgcag cgctacaacg
     4321 gcttcgtcgc ggcgcgcatc ctcggcaacg cgaaggaagg ctacagctcg ggccaggcga
     4381 tccgcgccgt cgaggagacc gcgaagaccg cgctgccgcc cggctacgac atggcctgga
     4441 ccggccaggc ctatcaagag aagcgcaccg gcaacgcctc ggtgttcgcg ttcggcttcg
     4501 cgatcgtgat ggtctacctg atcctgtcgg cgctgtacga gcgctggggc gtgccgttcg
     4561 cggtcgtgct cgcggtgccg ttcgcgctca ccggcgcgct gctgttcgtg tggctgcgcc
     4621 acatggagaa tgacatctac ttccagatcg gcctggtcgt gctgatcggg ctggcggcga
     4681 agaacgcgat cctgatcgcc gagttcgcga tgcaggggat ggaggccggc aagagcgccg
     4741 ccgaggccgc gcgcgacgcg gcgcggctgc gcttccggcc gatcgtgatg acctcgctgg
     4801 ccttcgtgtt cggcgtcgtg ccgctggtca ccgccaccgg cgccggcgcg gcggcgcgcc
     4861 agtcgatggg caccggcgtg ttcggcggca tgatcgtcgc gaccttcgtc gcgccgatct
     4921 tcgtgccgct gttcttcacg ctgctcgcgc gcaagccgcg gccgcggcac gagcaccacg
     4981 gggaagcgca ggcgctgcag gcgccgggcg gcgaagccgg aggctcgtcg tgaggcgcgg
     5041 cgcgatgctg atcgccacgg cctgcgccgc gctgctcgc
//
LOCUS       DASRDG010000641         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1482103, whole genome shotgun sequence.
ACCESSION   DASRDG010000641 DASRDG010000000
VERSION     DASRDG010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482103"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /gene="map"
                     /locus_tag="VFA35_10760"
     CDS             complement(<1..849)
                     /gene="map"
                     /locus_tag="VFA35_10760"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HZT56699.1"
                     /translation="MKPIDLDKLRRSGRISATALRHGRAMIAPGARIEDIARACEQVI
                     LDLGGKPAFPAQLSRNHIAAHYCSPPGDPTTLQPDDIVKLDLGTQVDGYVTDNATTVD
                     LRDGDGSLLVAASRMALDNAIAVMGPGASLTEIGRQIEGTIKALGFNPVYNLTGHGVA
                     RYVIHCAPSVPNYPDSKAGRLRANMTIACEPFACDGRGSIEVNGAAEVFMLRREIRPK
                     DSVPADVIEAVAVTEGLPFARRQLQPRLGSPARVDEAVALLRKKGLIEEYPPLCEKRG
                     VRVAQTE"
     gene            complement(872..>2026)
                     /locus_tag="VFA35_10765"
     CDS             complement(872..>2026)
                     /locus_tag="VFA35_10765"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZT56700.1"
                     /translation="ELDEIRAQGLWKEERVIASPQGARITSNGKEVLCLCANNYLGLA
                     NDADLRAAAKKAVDSHGLGLASVRFICGTQDIHKTLEQKVSRFLGTEDTILYGSCFDA
                     NGGLFETLMGEQDAIISDALNHASIIDGIRLSKAQRFRYQNGDMKELEQHLQATQSCR
                     SRLIATDGVFSMDGYVARLPEICALADKYQAMVMVDDSHATGFFGPTGRGTPEFHGVQ
                     KRIDILTSTLGKALGGAAGGFTTGRKEIVDLLRNRSRPYLFSNTLAPAIAGATIACLD
                     KLSSTTALCSKLAKNTQFFRAAMAKAGFAIVPGEHPICPIMLGEATVAVDMARRMLDE
                     GVYVIGFSYPVVPKGKARIRVQISAAHEIPQLETAVAAFAKVGRAMGIVK"
BASE COUNT          261 a          687 c          734 g          344 t
ORIGIN      
        1 ctcggtctgc gcgacgcgca cgccccgctt ctcgcacagc ggcgggtact cctcgatcag
       61 gcccttcttg cgcaacagcg cgaccgcctc gtcgacgcgc gcggggctgc ccagccgcgg
      121 ctgcagctgg cggcgcgcga acggcaggcc ctccgtgacc gcgaccgcct cgatcacgtc
      181 ggcgggcaca ctgtccttgg gccggatctc gcgccgcagc atgaacacct cggcggcgcc
      241 gttgacctcg atgctgccgc ggccgtcgca cgcgaacggc tcgcacgcga tcgtcatgtt
      301 ggcgcgcagg cggccggcct tgctgtcggg atagttgggc accgacggcg cgcagtggat
      361 cacgtagcgc gcgacgccgt ggccggtgag gttgtagacg gggttgaagc cgagcgcctt
      421 gatcgtcccc tcgatctggc ggccgatctc cgtcagcgac gcgccggggc ccatcacggc
      481 gatcgcgttg tcgagcgcca tgcggctcgc cgcgaccagc agcgaaccgt cgccgtcgcg
      541 caggtcgacg gtggtcgcgt tgtcggtcac gtagccgtcg acctgcgtgc cgaggtcgag
      601 cttgacgatg tcgtcgggct gcagcgtcgt cgggtcgccg ggcggcgagc agtagtgcgc
      661 ggcgatgtgg ttgcgcgaca gctgggccgg gaacgccggt ttgccgccga ggtcgaggat
      721 cacctgttcg caggcgcgcg cgatgtcctc gatgcgcgcg cccggcgcga tcatcgcgcg
      781 gccgtggcgc agtgccgtgg cgctgatgcg gcccgaacgg cgcagcttgt cgaggtcgat
      841 cggcttcatc gcccggtcat ttcacgagcg gtcacttcac gatccccatc gcgcggccga
      901 ccttggcgaa cgcggcgacc gccgtctcga gctgcgggat ctcgtgcgcg gcgctgatct
      961 gcacgcggat gcgcgccttg cccttgggca ccacggggta gctgaagccg atcacgtaga
     1021 cgccttcgtc gagcatgcgc cgcgccatgt cgacggcgac ggtcgcctcg ccgagcatga
     1081 tcgggcagat cgggtgctcg ccgggcacga tcgcgaagcc cgccttcgcc atcgccgcgc
     1141 ggaagaactg cgtgttcttc gccagcttcg agcaaagcgc ggtggtcgag gacagcttgt
     1201 cgaggcacgc gatcgtggcg ccggcgatcg ccggcgcgag cgtgttggag aacaggtacg
     1261 gccggctgcg gttgcgcagc aggtcgacga tctccttcct gccggtcgtg aagccgccgg
     1321 cggcgccgcc gagcgccttg cccagcgtcg acgtgaggat gtcgatgcgc ttctgcacgc
     1381 cgtggaactc cggcgtgccg cggccggtcg ggccgaagaa gccggtggcg tggctgtcgt
     1441 cgaccatcac catcgcctgg tacttgtccg cgagcgcgca gatctccggc agcctggcga
     1501 cgtagccgtc catcgagaac acgccgtcgg tggcgatcag gcgcgagcgg cagctctgcg
     1561 tcgcctgcag gtgctgctcc agctccttca tgtcgccgtt ctggtagcgg aagcgctgcg
     1621 ccttgctgag ccggatgccg tcgatgatcg acgcgtggtt gagcgcgtcg ctgatgatcg
     1681 cgtcctgctc gcccatcagc gtctcgaaca ggccgccgtt ggcgtcgaag cacgagccgt
     1741 agaggatcgt gtcctcggtg ccgaggaagc ggctgacctt ctgctcgagg gtcttgtgga
     1801 tgtcctgcgt gccgcagatg aagcgcacgc tcgcgaggcc gagcccgtgc gagtcgacgg
     1861 ccttcttcgc ggcggcgcgg aggtcggcgt cgttggcgag gccgaggtag ttgttggcgc
     1921 agaggcagag cacctccttg ccgttgctcg tgatgcgcgc gccctgcggg ctcgcgatca
     1981 cgcgctcctc cttccacagg ccctgggcgc ggatctcgtc gagttc
//
LOCUS       DASRDG010000642         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1024662, whole genome shotgun sequence.
ACCESSION   DASRDG010000642 DASRDG010000000
VERSION     DASRDG010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024662"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFA35_10770"
     CDS             <1..701
                     /locus_tag="VFA35_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HZT56701.1"
                     /translation="AHAAGAGRVYVELLDHSPTVMRVDPIRPELMFRGDWAGTSADAI
                     ARTTVVCGCGGGDAVRAPLPRLLSLARRLVLDADALNALAADTALATLLRARTARGHA
                     TVLTPHPLEAARLLACSSAEVQADRLRAVQALADGFACVVVLKGSGSVIAAPQQVARI
                     NSTGNASLASAGTGDVLAGWLGGRWTQHAPGDEAATAFKVAQQAVAEHGAAAEPAPHG
                     ALRAGDLIERLAAL"
     gene            complement(718..>2230)
                     /locus_tag="VFA35_10775"
     CDS             complement(718..>2230)
                     /locus_tag="VFA35_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HZT56702.1"
                     /translation="IDADAYERATSVYFPRRVIPMLPEKLSNGLCSLNPYEDRLAMVC
                     DMLVTAGGEIHAYQFFPAVICSHARFTYNEVAAILANTRGPEAAKRQELVPDLLHLHE
                     VYRALLKERNKRGAIDFETTETQIVCDENGRIEKIVPRTRTEAHKLIEEAMLAANVCA
                     ADFIESAKHPSLYRVHEGPTPEKKTLLQNYLRALGLGLSIGDDPKPGEFQAIAHATKD
                     RPDAMQIHTMLLRSMQQAIYTGVNSGHFGLAYEAYTHFTSPIRRYPDLLVHRVIKALL
                     GGKRYHLVTGASNPAPSNRKTTKPVRQATAEEEKWEVAGVHCSVNERRADEASRDVEA
                     WLKCRYMREHLGEEYGGTVSAVTSFGLFVQLDGLYVEGLVHITELGGEYYRFDEVRQE
                     LRGERSGVRYAVGSRVRVQVSRVDLDGRKIDFRMVRDGEDDASLLRAKRDKANATAAG
                     EPAHVKETDRAVKAVTKKNAQKARSSGAGGAAHPVRAAKSTARKAGGKASTRR"
BASE COUNT          271 a          792 c          796 g          371 t
ORIGIN      
        1 ccgcgcacgc ggccggtgcg gggcgcgtct acgtcgaact gctcgaccat tcgccgacgg
       61 tcatgcgcgt cgatccgatc cggcccgaat tgatgttccg cggcgattgg gccggcacca
      121 gcgccgacgc catcgcgcgc accaccgtcg tctgcggttg cggcggcggc gacgcggtgc
      181 gcgcgccatt gccgcggctg ctgagcctgg cgcgccgcct ggtgctcgac gccgacgcgc
      241 tcaacgcact cgccgccgac accgcgctcg cgacgctgct gcgcgcgcgc accgcgcgcg
      301 gccacgcgac cgtgttgacc ccgcacccgc tggaagccgc gcgcctgctt gcctgctcga
      361 gcgccgaggt tcaggccgat cgcctgcggg cggtgcaggc gctggccgac ggtttcgcct
      421 gtgtcgtcgt gctgaaggga tcgggcagcg tgatcgccgc gccgcagcag gtggcgcgca
      481 tcaacagcac cggcaacgcg tcgctcgcca gcgccggcac cggcgatgtg ctcgccggct
      541 ggctcggcgg gcgttggacg caacacgcgc cgggcgacga ggccgcgacg gcattcaagg
      601 ttgcgcagca ggcggtcgcc gaacacggcg cggccgccga acccgcgccg cacggcgcgc
      661 tgcgcgccgg cgacctgatc gagcgactcg ccgccctgtg acccgcgcgg cgccggctca
      721 gcgccgcgtc gacgccttgc cgccggcctt gcgcgccgtc gacttggccg cccgcaccgg
      781 atgcgccgcg ccgccggcgc cactgctgcg cgccttctgc gcgttcttct tggtcaccgc
      841 cttgacggcg cgatcggtct ccttgacgtg agccggctcg cccgcggccg tcgcgttggc
      901 cttgtcgcgc ttggcgcgca acaggctcgc gtcgtcttcg ccgtcacgca ccatgcggaa
      961 gtcgatcttg cgcccgtcga ggtcgacgcg gctgacttgc acccgcacgc gcgagccgac
     1021 cgcgtagcgc acgccgctgc gctcgccgcg cagctcctgc cgcacttcat cgaagcggta
     1081 gtactcgccg ccgagttcgg tgatgtgcac gaggccctcg acgtaaaggc cgtcgagctg
     1141 cacgaacagg ccgaagctcg tgaccgcgct gaccgtgccg ccgtactcct cgccgaggtg
     1201 ctcgcgcatg tagcggcact tcagccaggc ctcgacatcg cgcgaggcct cgtcggcgcg
     1261 gcgctcgttg acgctgcaat gcacgccggc gacttcccac ttctcttctt cggcggtggc
     1321 ctggcgcacc ggcttggtcg tcttgcggtt gctcggcgcg gggttgctcg cgccggtgac
     1381 gaggtggtag cgcttgccgc cgagcagggc cttgatgacg cggtgcacca gcaggtcggg
     1441 atagcgccgg atcgggctcg tgaagtgcgt gtaggcctcg tacgcaagcc cgaagtggcc
     1501 gctgttgacg ccggtgtaga tcgcctgctg catcgagcgc agcagcatcg tgtggatctg
     1561 catcgcgtcg gggcgatcct tggtcgcgtg cgcgatcgcc tggaactcgc cgggcttcgg
     1621 atcgtcgccg atgctcaggc cgaggccgag cgcgcgcagg tagttctgca gcagcgtctt
     1681 cttctcgggc gtcggccctt cgtgcacgcg gtagagcgac gggtgcttgg cgctctcgat
     1741 gaagtcggcc gcgcacacgt tcgccgcgag catcgcctcc tcgatcagct tgtgggcctc
     1801 ggtgcgcgtg cgcggcacga tcttctcgat gcggccgttc tcgtcgcaga cgatctgcgt
     1861 ctcggtggtc tcgaagtcga tcgcgccgcg cttgttgcgt tccttcagca gcgcgcggta
     1921 aacctcgtgc aggtgcagca ggtcgggcac cagttcctgg cgcttcgcgg cctcggggcc
     1981 gcgcgtgttc gcgaggatcg cggcgacctc gttgtaggtg aagcgcgcgt gcgagcagat
     2041 caccgccggg aagaactggt acgcgtggat ctcgccgccg gcggtgacca gcatgtcgca
     2101 gaccatcgcg aggcggtctt cgtacgggtt cagcgagcac aggccgttgc tgagcttctc
     2161 gggcagcatc gggatcacgc ggcgcgggaa gtagaccgag gtggcgcgct cgtaggcgtc
     2221 ggcatcgatc
//
LOCUS       DASRDG010000643         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1359793, whole genome shotgun sequence.
ACCESSION   DASRDG010000643 DASRDG010000000
VERSION     DASRDG010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359793"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFA35_10780"
     CDS             complement(<1..339)
                     /locus_tag="VFA35_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56703.1"
                     /translation="MRLPHLAALLLPTIAFAQQQTGTTEPARPEPGKLTPVAVTAVPA
                     ADSPEAAALFDKACAKMQAVGRGAFTTTEEQDPAMMRGHGMQGMGDQSVEVNGGWSGD
                     LVWGTVGEDEF"
     gene            complement(541..984)
                     /gene="rbfA"
                     /locus_tag="VFA35_10785"
     CDS             complement(541..984)
                     /gene="rbfA"
                     /locus_tag="VFA35_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZT56704.1"
                     /translation="MNDRRLQRLQEQIKARVAEILQRDLQDPKLGIVTVSRVELDREF
                     TQCTVYWSALGSDKEQRRTQAALQRARSFVQRAVGVTLHTRTVPRLLFVFDPSIAGAI
                     KMEGLLKELQRERQARTGEAPAPEGPPPDVQPDVPPDWPPDRPRP"
     gene            complement(1007..1300)
                     /locus_tag="VFA35_10790"
     CDS             complement(1007..1300)
                     /locus_tag="VFA35_10790"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HZT56705.1"
                     /translation="MLHIGVLQFTLEIPYAESLKDKRNVIKALKDRLRRSFNVSIAEV
                     DDLDECTVATLGAVVAGSDVPHLNSTLDHLLNTLQDWRDGTLADHQLEIFTPQ"
     gene            complement(1366..>2333)
                     /locus_tag="VFA35_10795"
     CDS             complement(1366..>2333)
                     /locus_tag="VFA35_10795"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZT56706.1"
                     /translation="VVESKQSPEQGVVVTVLVTDGTLRVKDQVVCGESFSRVRAMIDD
                     HGRQLQEAGPSTPVTVFGLDRLPNPGDKLFAVEDAKKAREVVEDRQRRIRELSMAERS
                     AVTLETLSQKLAERNLQEIKIILKADAMGSLEPLRKCLEELSTPEVRVNLVHSALGGI
                     NETDVSLAEASGAIIIGFNTVADTSARQAAERGGVEIRYFDIIYELLDQVKAAMEGLL
                     KPQEVESVVGHAEVRALFKSSKFGTIAGCYVTDGICKRSAKARLSRDGKVVYTGKVAS
                     LRREKDDAREVRAGFECGITLQDFQDLKVGDVIEFAQVDLVRRTL"
BASE COUNT          306 a          827 c          827 g          373 t
ORIGIN      
        1 gaactcgtcc tcgccgacgg tgccccagac gaggtcgccg ctccagccgc cgttcacctc
       61 gacgctctgg tcgcccatgc cttgcatgcc gtggccgcgc atcatcgccg ggtcctgttc
      121 ctcggtggtc gtgaacgcgc cgcggccgac ggcctgcatc ttggcgcagg ccttgtcgaa
      181 cagcgccgcg gcctcgggcg agtccgcggc gggcaccgcc gtcacggcga ccggggtgag
      241 cttgcccggc tcgggcctgg caggctcggt cgtgccggtc tgctgctggg cgaaggcgat
      301 ggtggggagg aggagggccg cgaggtgggg gaggcgcatg cgaggaaggg tagccaggag
      361 gcggaagggg tggcgagagc ggcgcccgac gaacgagtcc cgcgaaaacg cccaccgcgc
      421 cgcgaacggg caacagcggg cgggcagagc acccgggacg gtcgcggcgt cgggtcgcgc
      481 gtcagcgctg ccctgcggcc gcaaccacgt gccgcgcgcg agaacgcggc agaccctgga
      541 tcagggccgg gggcggtccg gcggccaatc cggcggcacg tccggttgca cgtccggcgg
      601 cggcccctcg ggggccggcg cctcgcccgt gcgggcctgc cgctcgcgct gcagctcctt
      661 cagcagccct tccatcttga tcgcgccggc gatcgacgga tcgaacacga acagcagccg
      721 cggcaccgtg cgcgtgtgca gcgtcacgcc gaccgcgcgc tgcacgaagc tgcgcgcgcg
      781 ctgcagggcc gcctgcgtgc ggcgctgctc cttgtcgctg ccgagcgccg accagtagac
      841 ggtgcactgc gtgaactcgc ggtcgagctc gacgcgggag acggtgacga tcccgagctt
      901 cgggtcctgc aggtcgcgct gcaggatctc ggcgacgcgc gccttgatct gctcctgcag
      961 gcgctggagg cggcggtcgt tcacggcgac tctccgccgg ccggcactac tggggcgtga
     1021 agatctcgag ctggtggtcg gcgagcgtgc cgtcgcgcca gtcctgcagc gtgttgagca
     1081 ggtggtccag cgtgctgttg aggtgcggca cgtcgctgcc cgcgaccacg gcgccgagcg
     1141 tcgccaccgt gcactcgtcg aggtcgtcga cttcggcgat cgacacgttg aacgagcgcc
     1201 gcagccggtc cttgagcgcc ttgatcacgt tgcgcttgtc cttgagcgat tccgcgtacg
     1261 ggatctccaa ggtgaactgc agcacgccga tgtgcagcac ggtcgttcac tccttgtcgg
     1321 ccatcctggc gggccattcg tttgtcggct cgcggcgcgg ccgggctaga gcgtgcggcg
     1381 cacgagatcg acctgcgcga actcgatcac gtcgccgacc ttgaggtcct ggaagtcctg
     1441 cagcgtgatg ccgcactcga aaccggcgcg gacctcgcgc gcgtcgtcct tctcgcggcg
     1501 caggctcgcg accttgcccg tgtagacgac cttgccgtcg cgcgacaggc gcgccttggc
     1561 gctgcgcttg cagatgccgt cggtgacgta gcagccggcg atcgtgccga acttgctcga
     1621 cttgaacagc gcgcgcacct cggcgtggcc gaccaccgac tcgacctcct gcggcttgag
     1681 caggccttcc atcgccgcct tcacctggtc gagcagctcg tagatgatgt cgaagtagcg
     1741 gatctcgacg ccgccgcgct cggctgcctg ccgcgcgctg gtgtcggcga cggtgttgaa
     1801 gccgatgatg atcgcgcccg acgcctcggc gaggctgacg tcggtctcgt tgatgccgcc
     1861 gagcgcgctg tgcacgaggt tgacgcgcac ctccggcgtg ctcagctcct cgaggcactt
     1921 gcgcagcggt tcgagcgaac ccatcgcgtc ggccttcagg atgatcttga tctcctgcag
     1981 gttgcgctcg gcgagcttct gcgacagcgt ctcgagcgtc accgcgctgc gctccgccat
     2041 cgacagctcg cggatgcgcc gctgccggtc ctcgaccacc tcgcgcgcct tcttcgcgtc
     2101 ctcgacggcg aacagcttgt cgccggggtt cggcagccgg tcgaggccga acacggtcac
     2161 cggcgtcgac ggacccgcct cctgcagctg gcggccgtgg tcgtcgatca tcgcccgcac
     2221 gcgcgagaag ctctcgccgc agacgacctg gtccttgacc cgcagcgtgc cgtcggtcac
     2281 cagcacggtg accaccacgc cctgctccgg cgactgcttg gactcgacca cca
//
LOCUS       DASRDG010000644         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1482473, whole genome shotgun sequence.
ACCESSION   DASRDG010000644 DASRDG010000000
VERSION     DASRDG010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482473"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFA35_10800"
     CDS             <1..239
                     /locus_tag="VFA35_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56707.1"
                     /translation="ECWSVYAWPVEYGVTGLRAFAITQHGDVLWTRNEVHFSGRQKPM
                     PPTAALRAEANGTLRGPYTVNDIAADGSTWGIIQ"
     gene            290..1084
                     /locus_tag="VFA35_10805"
     CDS             290..1084
                     /locus_tag="VFA35_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZT56708.1"
                     /translation="MPEPAPKSITSTKNPIVARFRAAATGDDAGALLAEGVRLVQEGL
                     DAGLAVHAALVSPRLREPALGERLRAVAAEFAECSDEVLARTSALETHQGVAAVFARP
                     RWQPADLLRRDLAPLVTAAAGVRDPGNLGALLRTAEAAAATGFVALAGGADPFREKAV
                     RGAMGSTFRLPTLAGMQPAELLAFCRQQKLQLVVADGVGERDYLDFDWRKPIVLVVGA
                     EAAGVPPQLLQAADARVRIAVRAPVDSLNVAVAAGVLLFEARRQRR"
     gene            1087..1956
                     /locus_tag="VFA35_10810"
     CDS             1087..1956
                     /locus_tag="VFA35_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56709.1"
                     /translation="MRAHLAAVLGLAACAAVDPDPQWLSHAPVGAEAAAARAVGFAVP
                     AALELGFDDKVAGGSIAAGDRVLFGVRLDRDGDAEVRFVQVEVVGVEVQQWELPFALA
                     GDGSAAPAVGQMPFARVHATVCDADGRALGDDTVLLHALELEHGLVAACRGDGPAETR
                     RAISALRSVLHVVRGSAALRALLWQVVDLPSLFSLLWHFGVKVSLAENFAGSQRGAAT
                     ALGAATVATWEAPLELRLNGAPALRCRVLVTDPDAPWALCAGIVAIQAASPSHPERTV
                     TMRLLGARRGAPP"
     gene            1953..>3076
                     /locus_tag="VFA35_10815"
     CDS             1953..>3076
                     /locus_tag="VFA35_10815"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZT56710.1"
                     /translation="MSLYGDEHAGAKAPAAPLAERMRPRTLAEFLGQEQIVGEGRALR
                     AAIERGAAGSLILWGPPGTGKTTLANLIAQHAGLAFVPFSAVLSGIAQVRESVAEAKE
                     RREQTGKGTLLFVDEIHRFNKSQQDAFLPHVESGLLVLIGATTENPSFAVNAALLSRC
                     RVVALQSLPDTAVAALVARALTDKERGLGARGLQLADDAQARLLVLAGGDARRALTAV
                     EACAQTCADGDTITAAMITEAFQHRALRHDKDGDLHYDLLSALHKSLRNSDVQAAVYW
                     LQRAIEAGADPLHAARRMVAMAAEDIGLADPMALQVAVAAQQAAHFLGLPEANLPLTQ
                     AAVYLAAAPKSNAVVSAISAAQAAARDGAQHPVPVHLRNAV"
BASE COUNT          370 a         1135 c         1184 g          387 t
ORIGIN      
        1 cagaatgctg gtccgtctac gcgtggcccg tcgaatacgg agtcacgggc ctgcgcgcgt
       61 tcgcgatcac ccagcacggc gacgtgctgt ggacgcgcaa cgaagtgcac ttcagcggcc
      121 ggcagaagcc gatgccaccg accgcggcac tgcgtgccga ggcgaacggg acgctgcgcg
      181 gcccctacac ggtcaacgac atcgctgccg acggcagcac ctggggcatc atccagtagc
      241 cggccgcgcg cccttctcac ggcgacgccg ccgcgtcaag atggcgccga tgccggaacc
      301 cgcgccgaag tcgatcacgt cgacgaagaa cccgatcgtc gcgcgcttcc gcgccgcggc
      361 gaccggcgac gacgccggcg cgctgctcgc ggagggcgtg cggctggtgc aggaaggcct
      421 cgacgcgggg ctcgccgtgc acgcggccct ggtgtcgccg cggctgcgcg aaccggcgct
      481 cggcgaacgg ctgcgcgccg tcgccgcgga gttcgccgag tgcagcgacg aggtgctcgc
      541 gcgcacgagc gcgctcgaga cgcaccaggg cgttgccgcc gtgttcgcgc ggccccgctg
      601 gcagccggcg gacctgttgc gccgcgatct cgccccgctc gtcacggccg ccgccggcgt
      661 gcgcgatccc ggcaacctcg gcgcgctgct gcgcacggcc gaggccgcgg cggcgaccgg
      721 gttcgtcgcg ctcgcgggcg gcgccgatcc gttccgcgag aaggcggtgc gcggcgcgat
      781 gggctcgacg ttccggctgc cgacgctcgc cggcatgcag cccgcggagc tgctcgcctt
      841 ctgccgccag cagaagctgc agctggtcgt cgccgacggc gtcggcgaac gcgactacct
      901 cgacttcgac tggcgcaagc cgatcgtgct cgtcgtcggc gccgaggccg ccggcgtgcc
      961 gccgcagctg ctgcaggccg ccgacgcgcg cgtgcgcatc gccgtgcgcg cgccggtcga
     1021 cagcctcaac gtcgccgtcg ccgccggcgt gctgctgttc gaggcgcggc gccagcggcg
     1081 gtgacgatgc gcgcgcacct cgcagcggtg ctggggctgg ccgcgtgcgc ggcggtcgat
     1141 cccgatccgc agtggctgtc gcacgcgcca gtgggggccg aagcggcggc ggcgcgtgcg
     1201 gtcgggttcg cggtgccggc ggcgctcgaa ctcggcttcg acgacaaggt cgcgggcggc
     1261 agcatcgccg ccggcgatcg cgtgctgttc ggcgtgcggc tcgaccgcga tggcgacgcc
     1321 gaggtccgct tcgtgcaggt ggaggtcgtc ggggtcgagg tccagcagtg ggagctgccg
     1381 ttcgcgctcg ccggcgacgg gtccgccgcg cccgcggtcg ggcagatgcc cttcgcccgc
     1441 gtgcatgcga cggtgtgcga cgccgacggc cgcgccctcg gcgacgacac cgtgctgctg
     1501 catgccctcg aactcgagca cgggctcgtc gctgcgtgcc gcggcgacgg gccggccgag
     1561 acgcggcgcg cgatcagcgc cctgcgcagc gtgctgcacg tcgtgcgcgg cagcgcggcg
     1621 ctgcgcgcgc tgctgtggca ggtcgtcgac ctgccgtcgc tgttctcgct gctctggcac
     1681 ttcggcgtga aggtgtcgct ggcggagaac ttcgccggca gccagcgcgg tgcggcaacg
     1741 gcgctcggtg ccgcgacggt ggcgacctgg gaggcgccgc tcgaactgcg gctcaacggc
     1801 gcgccggcgc tgcgctgccg cgtgctggtg accgaccccg acgcgccgtg ggcgctgtgc
     1861 gccggcatcg tcgcgatcca ggccgcgtcg ccgagtcatc cggagcgcac cgtgacgatg
     1921 cgcctgctcg gcgcgcggcg gggggcgccg ccgtgagcct ctacggcgac gaacacgcgg
     1981 gcgcgaaggc gccggccgcg ccgctcgccg agcgcatgcg accgcgcacg ctggccgagt
     2041 tcctgggcca ggagcagatc gtcggcgaag ggcgcgcgct gcgggccgcg atcgagcgcg
     2101 gcgccgccgg ctcgctgatc ctgtgggggc cgccgggcac cggcaagacg acgctcgcca
     2161 acctgatcgc gcagcatgcg gggctcgcgt tcgtgccgtt ctcggcggtg ctgtcgggca
     2221 tcgcgcaggt gcgcgagtcg gtcgccgagg cgaaggagcg gcgcgagcag accggcaagg
     2281 gcacgctgct gttcgtcgac gagatccacc gcttcaacaa gagccagcag gacgcgttcc
     2341 tgccgcacgt cgagtcgggg ctgctggtgc tgatcggcgc caccaccgag aacccgtcgt
     2401 tcgcggtcaa cgcggcgctg ctgtcgcgct gccgcgtggt cgcgctgcag tcgctgccgg
     2461 acacggcggt ggcggcgctg gtggcccgcg cgctcaccga caaggaacgc gggctcggtg
     2521 cgcgcggcct gcagctcgcc gacgacgcgc aggcacggct gctggtgctc gccggcggcg
     2581 acgcgcggcg cgcgctgacg gcggtcgagg cctgcgcgca gacctgcgcc gacggcgaca
     2641 cgatcaccgc ggcgatgatc accgaggcgt tccagcaccg cgcgctgcgc cacgacaagg
     2701 acggcgacct gcactacgac ctgctgtcgg cgctgcacaa gagcctgcgc aactccgacg
     2761 tgcaggcggc cgtctactgg ctgcagcgcg cgatcgaggc cggcgccgac ccgctgcacg
     2821 ccgcgcggcg catggtggcg atggcggccg aggacatcgg cctcgccgac ccgatggcgc
     2881 tccaggtcgc ggtcgcggcg caacaggcgg cgcacttcct cggcctgccc gaagccaacc
     2941 tgccgctgac gcaggcggcc gtctacctcg ccgcggcgcc gaagagcaac gcggtggtca
     3001 gcgcgatctc tgccgcgcag gcggcggcgc gcgacggcgc gcagcatccc gtgcccgtgc
     3061 acctgcgcaa cgcggt
//
LOCUS       DASRDG010000645         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_553707, whole genome shotgun sequence.
ACCESSION   DASRDG010000645 DASRDG010000000
VERSION     DASRDG010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553707"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFA35_10820"
     CDS             <1..640
                     /locus_tag="VFA35_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZT56711.1"
                     /translation="QDSPGFLVNRCLSPYLNEAARLLVEGNDPDDLDRVLLDFGMPMG
                     PCRLLDEIGFDVAAKVSEVMHKAFAERMQPCPLFAAMVEAKALGAKTGGGIRSKKGDG
                     PGPGMQVVQRLRRERGAPPRQASRGEVLQRLIYPMVDEAYRCLDDKIVAAEQDLDLGL
                     VMGIGFPPFLGGVVGFSKREGLRTIVGALDELARSVDARFAPSDGLRRRALS"
     gene            973..1917
                     /locus_tag="VFA35_10825"
     CDS             973..1917
                     /locus_tag="VFA35_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56712.1"
                     /translation="MTFDVSNRAILRELAHSSPMRLGVEFLPPAADESAVAVTPAAAR
                     AQDAPATAPARPQAPISPTGDPDDVRSDRHTWTLSIEDLSAMRTPLERETLQFLADLR
                     HADLRRQRRELGTPILTIETVDLESPELRLMQDDALADERAEWLTEHGFELFRRPASL
                     LLRRLPLVQGLQVELDDFKSDNVPLSAEYRQTHQDEPNLGRVSMRVRANDLHDPVEIA
                     YMKSGVRIGTSQGMLKLGYTHRLTDNIDFSVRPRLDYATGAWHVRADLGWTLSHTTSL
                     HLVAGDNLDFLATSNFYSLFDSPMDGSPGLLFYAVHLF"
BASE COUNT          303 a          809 c          743 g          333 t
ORIGIN      
        1 ccaggactcg cctggcttcc tcgtcaaccg ctgcctgtcg ccctacctca acgaggcggc
       61 gcgcctgctg gtcgagggca acgacccgga cgacctcgac cgcgtgctgc tcgacttcgg
      121 catgccgatg ggaccgtgcc gcctgctcga cgagatcggc ttcgacgtgg cggcgaaggt
      181 cagcgaggtg atgcacaagg cgttcgccga gcgcatgcag ccctgtccgc tgttcgcggc
      241 gatggtcgag gccaaggccc tcggcgccaa gacgggcggc ggcatccgca gcaagaaggg
      301 tgacggaccg ggccccggca tgcaggtcgt gcagcggctg cgcagggaac gcggcgcgcc
      361 gccgcgccag gcgtcgcgcg gcgaggtcct gcagcgcctg atctacccga tggtcgacga
      421 ggcctaccgc tgcctcgacg acaagatcgt cgccgccgaa caggacctcg atctcggcct
      481 cgtgatgggc atcggcttcc cgccgttcct cggcggcgtc gtcggcttca gcaagcgcga
      541 aggattgcgc acgatcgtcg gtgcactcga cgaactcgcg cggtccgtcg atgcgcgctt
      601 cgcgccgagc gacggattgc gccggcgcgc gctgagctga atcgagcgcg ccgaggcaac
      661 ggcgcgcgtc tgcgcacatc tgcggcgggg cccgataacg gatttctcga cctcgtcgcg
      721 cccatgccaa cgtaaggtcg gcgccgagac gaaactcgtc cttgtaatcg gtgtagaatg
      781 cgtgcacgcg cgcgcgagca gcggtgtagc gcgcgcattc gtgtgccgat aggcgagggc
      841 cataggggcc tctccgtcgc ggttcgtcac gcgttccttc gcatcgccgt ggtccgtgtc
      901 ttgaggttcc tgctgggcgc ttttctctgc ctggccgcct gcgcaacgca gtcgacctca
      961 gtcgatgcct cggtgacgtt cgatgtctcg aatcgagcca tcctgcgtga gctcgcgcac
     1021 agcagcccga tgcgcctcgg cgtcgagttc ctgccccccg ccgccgacga gagcgcggtc
     1081 gccgtcacgc cggccgcggc gcgtgcgcag gacgcgccgg cgacggcgcc ggcgaggccg
     1141 caggccccga tctcgcccac cggcgacccc gacgacgtgc gcagcgaccg gcacacgtgg
     1201 acgctcagca tcgaggacct ttcggcgatg cgcacgccgc tcgagcgcga gacgctgcag
     1261 ttcctcgccg acctgcgcca cgcggacctg cggcgccagc ggcgcgagct cggcacgccg
     1321 atcctgacca tcgagaccgt cgacctcgag agccccgagc tgcggctgat gcaggacgac
     1381 gcgctcgcgg acgagcgcgc cgaatggctc acggagcacg ggttcgagct gttccggcgc
     1441 ccggcgagcc tgctgctgcg ccggctgccg ttggtccagg ggctgcaggt ggagctcgac
     1501 gacttcaagt ccgacaacgt gccgctgtcc gcggagtacc gccagacgca ccaggacgag
     1561 cccaacctcg gccgcgtgtc gatgcgcgtg cgcgcgaacg acctccacga tcccgtcgag
     1621 atcgcctaca tgaagtccgg cgtgcgcatc ggcacgagcc aggggatgct caagctcggc
     1681 tacacgcatc ggctgacgga caacatcgac ttctcggtcc gcccgcgcct cgactacgcc
     1741 accggcgcct ggcacgtgcg ggccgacctc ggctggacgc tgtcgcacac gacgagtctg
     1801 cacctggtcg cgggcgacaa tctggacttc ctggcgacct ccaacttcta ctccctgttc
     1861 gactcgccga tggacggttc cccggggctc ctgttctatg ccgtgcacct gttctagcgg
     1921 cagccgccgc aggagcgctg ccgttgcggc gaccgccgcc gcgggtgcct ggctcgccgc
     1981 gtgctcgtac gtgccgccgc cgtcgttccc gcgtcccgct ctgccggcgc cgcccgcggt
     2041 gtcggcccgc tacgcgctgc cggagcctcc tcgcctgctg cacctcgacc agcgctcggt
     2101 cgacggcgcc gccgacggcg agctcgcctg cggcgcggag accatccatt tccatctgca
     2161 gcgcgcgccg gaccccgacc ggccggtg
//
LOCUS       DASRDG010000646         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_100656, whole genome shotgun sequence.
ACCESSION   DASRDG010000646 DASRDG010000000
VERSION     DASRDG010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100656"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="VFA35_10830"
     CDS             <1..1561
                     /locus_tag="VFA35_10830"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZT56713.1"
                     /translation="VPGLPTLHAALLAGVATVLSLAVTRFMSRVERHLVDRADGLERS
                     LRQANSDLQKIAYHDALTGLPNRLVFEDKLLAAVGRADQAKARLAVLFIDLDGFKPIN
                     DSFGHSSGDVVLRQVGDRLRRLSRSADTMARVGGDEFLMLLEGEVDEQSASQVAARML
                     ATLGEPYSIGSREVTVSCSIGIVFYPDGGASPKLIARADAAMYAAKRSGGACYCFFEP
                     SMEADAREQLDLQRDLRLALERNELELFYQPKVDAHSGKVTAAEALLRWKHPTKGMIG
                     PAMFIPVAERFGLIGTLGNWVIEDACRQSRAWRDKGLRMRVAINLSAFQMRQDDLVER
                     ITTALARHRIDPSLLTCEITESVAMEDTKATQTTFKRLGEAGVHLSIDDFGTGYSSLG
                     YLRKLPAEELKIDRSFVMDIETSADARAVVDAVIHLAHALGLKVVAEGVETERQRDVL
                     IQMGCDELQGYLFARPMSARALLLWASDDREREVAFKPSLFGETRPVTTDEVLAQRRS
                     AATTVPLRLDF"
     gene            complement(1600..2664)
                     /locus_tag="VFA35_10835"
     CDS             complement(1600..2664)
                     /locus_tag="VFA35_10835"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56714.1"
                     /translation="MRKTATPFPTLITDAPESWQWPAPPIAWRHRPAPGLDEVQPCRI
                     ESPNGTPVDGEMLGFDPGKRTLTFRSSATGPVVELSFARFRRLTLTEPLRPVVQAAGT
                     PVEQVRAAAHERDYRLRSNSTAPAISGRTAGRVEAPEGLYLFAPVEEEASLQRVFVPR
                     SAYTLCEFGASAEEVAARMWIASPTELLAAIERQQRMPIRPLGQSMLELGLLTQTQLE
                     RALARQTGDRPLGESLVAGGLISRNDLQTALAHKMGYPRVDLARFPIDPQAIAKLPQR
                     IAVGFRLMPLMLDGDRLIVAVDKPARVVKLRTVHAIAGITVVPVLASRMQILAAQERE
                     THDIWSGHVSERLGFFSTTV"
     gene            complement(2801..3226)
                     /locus_tag="VFA35_10840"
     CDS             complement(2801..3226)
                     /locus_tag="VFA35_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HZT56715.1"
                     /translation="MKLEKILYTAHATSTGGREGTSKSSDGVLDVKLTTPKEMGGSGA
                     VGTNPEQLFAAGYSACFIGAMKYVAMTQKIALPADLSITADVGIGPIPAGFGIQVTLN
                     IAVPGMERAAAEKLVEAAHGVCPYSNATRGNIDVTLKVA"
BASE COUNT          495 a         1108 c         1174 g          500 t
ORIGIN      
        1 cgtgccgggc ttgccgacct tgcatgcagc cttgctcgca ggtgtcgcga ccgtgctgtc
       61 gctcgccgtg acacgcttca tgtcgcgcgt cgaacgccac ctggtcgacc gcgccgacgg
      121 cctcgagcgc tcgctgcgcc aggccaattc cgacctgcag aagatcgcgt accacgacgc
      181 gctcaccggc ttgccgaacc ggctggtgtt cgaggacaag ctgctcgccg cggtcggtcg
      241 ggccgaccag gcgaaggcgc gcctggcggt gctgttcatc gacctcgacg gcttcaagcc
      301 gatcaacgac tcgttcggcc acagcagcgg cgacgtcgtg ctgcgccagg tcggcgaccg
      361 gctgcggcgc ctgtcgcgct cggccgacac gatggcgcgc gtcggcggcg acgagtttct
      421 gatgctgctc gaaggcgagg tcgacgagca gagcgcctcc caggtcgccg cgcgcatgct
      481 ggccacgctc ggcgagccgt attcgatcgg cagccgcgag gtcacggtgt cgtgttcgat
      541 cggcatcgtg ttctacccgg acggtggcgc gagccccaag ctgatcgcgc gcgccgacgc
      601 cgcgatgtac gcggccaagc gctcgggcgg cgcctgctac tgcttcttcg agccgagcat
      661 ggaggccgac gcgcgcgagc agctcgacct gcagcgcgac ctgcgcctgg cgctggagcg
      721 caacgagctc gagctgttct accaacccaa ggtcgatgcc cacagtggca aggtcacggc
      781 ggccgaggca ctgctgcgct ggaaacaccc gaccaagggc atgatcggcc cggccatgtt
      841 catcccggtg gccgagcgct tcggcctgat cggcaccctc ggcaactggg tcatcgaaga
      901 cgcctgccgc cagtcgcgcg catggcgcga caagggcttg cgcatgcgcg tcgcgatcaa
      961 cctgtcggcg ttccagatgc gccaggacga cctggtcgag cgcatcacga cggcgctggc
     1021 gcggcaccgc atcgacccgt cgctgctgac ctgcgagatc accgagtcgg tggcgatgga
     1081 agacaccaag gccacgcaga cgaccttcaa gcgcctcggc gaagccggcg tgcacctgtc
     1141 gatcgacgac ttcggcaccg gctattcgag cctgggctac ctgcgcaagc tgccggccga
     1201 ggagctgaag atcgaccgca gcttcgtgat ggacatcgag accagcgccg atgcgcgcgc
     1261 ggtcgtcgac gcggtgatcc acctcgcgca cgcgctcggc ctgaaggtgg tggccgaagg
     1321 cgtcgagacc gagcgccagc gcgacgtgct gatccagatg ggctgcgacg agctgcaggg
     1381 ctacctgttc gccaggccga tgtcggcgcg ggcgctgctg ctgtgggcga gcgacgatcg
     1441 cgagcgcgag gtcgccttca agccgtcgct gttcggcgag acccggccgg tgaccacgga
     1501 cgaggtactg gcccagcgcc gcagcgccgc gacgacggtg ccgctgcggc tcgacttcta
     1561 ggagagtgtc gggcctgaga cgggcctgcg ccgggcggct cagacggtgg tcgagaagaa
     1621 cccgagacgt tcggacacgt gcccggacca gatgtcgtgc gtctcgcgct cctgcgccgc
     1681 cagaatctgc atccgcgacg ccagcaccgg caccacggtg atcccggcaa tggcgtgaac
     1741 cgtcctgagc ttgacgaccc gcgccggctt gtcgaccgcg acgatcagcc ggtcgccgtc
     1801 gagcatcagc ggcatcagcc ggaacccgac ggcgatgcgt tgcggcagtt tggcgatcgc
     1861 ctgcggatcg atcggaaagc gggccagatc gacccgcgga tagcccatct tgtgcgccag
     1921 cgcggtctgc agatcgttgc gcgagatcag gccgccggcc accagtgact cgcccagcgg
     1981 ccggtcgccg gtctggcggg ccagcgcgcg ctcgagctgg gtctgcgtca gcaagcccag
     2041 ctccagcatc gactggccga gcgggcggat cggcatgcgc tgctggcgct cgatcgccgc
     2101 gagcaactcg gtcgggctcg cgatccacat gcgcgcggcc acctcttcgg cggaagcgcc
     2161 gaactcgcac agggtgtagg ccgagcgcgg cacgaacacg cgctgcagcg aggcctcttc
     2221 ttcgaccggc gcgaacaggt agagcccttc gggtgcctcg acgcggccgg cggtgcgccc
     2281 gctgatggcg ggtgcggtgc tgttcgaccg caagcggtag tcgcgctcgt gcgcggcggc
     2341 gcgaacctgc tcgaccggcg tcccggcggc ctgcacgacc gggcgcaacg gctcggtcag
     2401 cgtgagccgg cgaaagcgcg caaatgaaag ttccaccacc gggccggtgg ccgaactgcg
     2461 gaacgtcagc gtgcgcttgc cggggtcgaa gccgagcatc tcgccgtcca ccggggtgcc
     2521 gttcgggctc tcgatgcggc acggctgtac ctcatcgagg ccgggcgccg gccggtgccg
     2581 ccacgcgatg ggcggcgccg gccattgcca gctttcgggg gcgtcggtga tcagggtcgg
     2641 aaacggggtc gcggtcttgc gcatggcggt cggacagagt agggcagggt tgcggcagcc
     2701 aaggccgagg ctgttatcca gcctacatcc gccagcgctg ccgggtaccg tgaaaaagcc
     2761 gcggatgccg ccgcatttgc ggcgatccgc tcggcgagcc tcaggcgacc ttcaacgtga
     2821 cgtcgatgtt gccgcgcgtc gcgttcgagt acgggcacac gccgtgcgcg gcttcgacca
     2881 gcttctcggc ggcggcgcgc tccatgccgg gcaccgcgat gttcagcgtg acctggatgc
     2941 cgaagccggc cgggatcggg ccgatgccga cgtcggcggt gatcgacaag tcggccggca
     3001 gcgcgatctt ctgtgtcatt gcaacgtact tcatcgcgcc gatgaagcag gccgagtagc
     3061 ccgccgcgaa caactgctcg gggttggtgc cgacggcgcc gctgccgccc atttccttcg
     3121 gcgtggtcag cttcacgtcg agcacgccgt cggaggactt cgaggtgcct tcgcgcccgc
     3181 cggtggaggt ggcgtgggcg gtgtagagaa tcttttcgag cttcatggtg cgttcctctg
     3241 gttgaacggg tgggggaagg gatcgggcga gcgcatg
//
LOCUS       DASRDG010000647         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_554286, whole genome shotgun sequence.
ACCESSION   DASRDG010000647 DASRDG010000000
VERSION     DASRDG010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554286"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VFA35_10845"
     CDS             <1..165
                     /locus_tag="VFA35_10845"
                     /inference="COORDINATES: protein motif:HMM:NF018249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HZT56716.1"
                     /translation="PNETTLQHLDPAPGQTAGGFAETVQNIELVGSRVVDHIVGNWAQ
                     WQAGVPALKP"
     gene            complement(187..693)
                     /gene="ispF"
                     /locus_tag="VFA35_10850"
     CDS             complement(187..693)
                     /gene="ispF"
                     /locus_tag="VFA35_10850"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZT56717.1"
                     /translation="MNAPRIRPPLWRIGEGWDTHALVAGRKLVLGGVEIPHTHGLLGH
                     SDADALCHAITDALFGAAAMGDIGFHFPDTDTQYQGADSLALLAEAARRVRAAGWLIG
                     NVDSTIIAQAPKMAPHILAMRQRVAQAIGVQLDQVSVKAKTAEKMGPVGNGEAIEARA
                     VCLIAKAA"
     gene            complement(690..1394)
                     /gene="ispD"
                     /locus_tag="VFA35_10855"
     CDS             complement(690..1394)
                     /gene="ispD"
                     /locus_tag="VFA35_10855"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZT56718.1"
                     /translation="MTLALTSEAPPVLYALVPCAGTGERAGAAGPKQYAMLGERSLVA
                     HTLAALAQVARINEVLVVVSPGDTQIDAHLDGRASVARCGGASRAETVRNGLDALLAR
                     GARAHDWVLVHDAARCLVRPEWVDALIDACVDDRVGGLLALPVADTLKAEHAGRVEAT
                     IDRRAKWQAQTPQMFRIGLLREALAHTGASVTDEASAIEALGHAPKLVACSMENFKIT
                     WPADFDLAARLLRTRP"
     gene            1493..>2572
                     /locus_tag="VFA35_10860"
     CDS             1493..>2572
                     /locus_tag="VFA35_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZT56719.1"
                     /translation="MQLPAIAAGKRFTLPRPTGSADALLLAKFAQTQAAQEKITAIIT
                     AEPADTQRLEAELPFFAPGLRIAVFPDWETLPYDTFSPHQDLISERLATLWRIQQGDV
                     DVVLMPASTALVRLAPPAFLARYTFHFKQKQKLNEAALKAQLLLAGYTHVSQVVSPGE
                     YAVRGGLIDLFPMGSLVPYRVDLFGDEVDSIRTFDPDTQRSLFPVPEVRLLPGREFPM
                     DEDARTRFRARWREKMEGDPSRVRLYKDIGTGLASGGIEYYLPLFFDDTATIFDYLGE
                     SATLVLHGELDEALKKFWTDTRERHRFLQADRERPILPPEDLFLKPEDFFARCNEHAQ
                     LALRGIEATEWARVLPDLGVDRGAPE"
BASE COUNT          380 a          911 c          869 g          412 t
ORIGIN      
        1 ccgaacgaga cgaccttgca gcatctcgac ccggcgccgg gccagaccgc cggcgggttt
       61 gccgagacgg tgcagaacat cgagctggtc ggctcgcgag tcgtcgacca catcgtgggc
      121 aactgggcgc agtggcaggc gggcgtgccg gccctcaagc cctgacgcgc cacggcgccg
      181 gccggatcag gcggccttcg cgatcaggca gacggcgcgc gcttcgatcg cctcgccatt
      241 gccgaccggc cccatcttct cggccgtctt cgccttcacg ctgacctggt cgagctgcac
      301 gccgatcgcc tgcgcaacgc gctgccgcat ggccagaatg tgcggcgcca tcttcggcgc
      361 ctgcgcaatg atcgtgctgt cgacgttgcc gatcagccag ccggccgcgc gcacgcggcg
      421 cgccgcttcg gccagcagcg cgagcgagtc ggccccctga tactgcgtgt cggtgtcggg
      481 aaagtgaaag ccgatgtcgc ccatcgcggc ggcgccgaac agggcatcgg tgatcgcatg
      541 gcacagcgcg tcggcgtcgg aatggccgag caggccgtgc gtgtgcggta tctcgacgcc
      601 gccgagcacg agcttgcggc cggcgaccaa cgcatgcgtg tcccagcctt cgccgatgcg
      661 ccagagaggc ggcctgatcc tgggtgcgtt catgggcgtg ttctcagcag tcgagcagcc
      721 aggtcgaagt cggcgggcca ggtgatcttg aagttctcca tcgagcatgc gacgagcttg
      781 ggtgcgtggc cgagcgcctc gatcgcgctg gcttcgtcgg tgacgctcgc gccggtgtgg
      841 gccagtgcct cgcgcagcag gccaatgcgg aacatctgcg gcgtctgcgc ctgccacttc
      901 gcgcggcggt cgatcgtggc ttcgacgcgg ccggcgtgct cagccttcag cgtatcggcg
      961 accggcagcg cgagcagccc gccgacgcgg tcgtccacac aggcgtcgat caacgcgtcg
     1021 acccattcgg ggcgaaccag gcagcgcgcg gcgtcgtgca ccagcaccca gtcgtgcgct
     1081 cgcgcgccgc gcgcgagcaa cgcgtcgagg ccgttgcgca cggtttcggc gcggctcgca
     1141 ccgccgcagc gggcgacgct cgcacggccg tcgagatgcg catcgatctg cgtgtcgccc
     1201 gggctcacca cgaccagcac ctcgttgatc cgcgcgacct gtgccagagc ggccagcgtg
     1261 tgtgccacca ggctgcgctc accgagcatc gcgtactgct tcggccccgc ggccccggcg
     1321 cgctcgcccg tgcccgcgca ggggacgagt gcatagagaa ccggcggggc ttcggaggtg
     1381 agggcaagcg tcattggccc gcgaattcta taatcggcac cagtttccgc cccggggatg
     1441 ccacgtccag acggccccgc ggggcgcttt gctttttgtg cgccaaggat tcatgcaact
     1501 ccccgcgatc gccgccggaa aacgcttcac gctgccccgc cccaccggtt cggccgatgc
     1561 actgctgctg gcgaaattcg cccagacgca ggcggcgcaa gagaagatca ccgcgatcat
     1621 caccgccgag cccgccgaca cgcagcgcct cgaggccgag ttgccgttct tcgcgccggg
     1681 gctgcgcatc gccgtgtttc ccgattggga gacgctgccc tacgacacct tctcgccgca
     1741 ccaagatttg atctcggagc ggctcgccac cttgtggcgc atccagcaag gcgatgtcga
     1801 tgtggtgctg atgccggctt cgaccgcgct cgtgcgcctc gcgccaccgg cctttctcgc
     1861 ccgctacacc ttccacttca agcagaagca gaagctcaac gaggccgcgc tgaaggcgca
     1921 gctcctgctt gccggctaca cccacgtgag ccaggtcgtc tcgcccggcg agtacgcggt
     1981 gcgcggcggg ctgatcgacc tgttcccgat gggttccctg gtgccgtacc gcgtcgacct
     2041 gttcggcgac gaggtcgact cgatccgcac cttcgatccc gatacccagc gcagcctgtt
     2101 cccggtgccc gaggtgcggc tgctgccggg ccgcgagttc ccgatggacg aggacgcgcg
     2161 cacgcgcttt cgcgcccgtt ggcgcgagaa gatggagggc gatccttcgc gcgtgcggct
     2221 gtacaaggac atcggcaccg ggctggcgag cggcggcatc gagtactacc tgccgctgtt
     2281 cttcgacgac accgcgacga tcttcgatta cctcggcgag agcgcgacgc tcgtcttgca
     2341 cggcgaactc gacgaggcgc tgaagaagtt ctggaccgac acgcgcgagc ggcatcgctt
     2401 tctgcaggcc gatcgcgaac ggccgatcct tccgcccgaa gacctgttcc tgaagcccga
     2461 ggatttcttc gctcggtgca acgagcatgc gcaactcgcg ctgcgcggca tcgaggccac
     2521 ggagtgggcg cgcgtgctgc ctgatctcgg ggtcgaccgc ggcgcgcccg ag
//
LOCUS       DASRDG010000648         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_214329, whole genome shotgun sequence.
ACCESSION   DASRDG010000648 DASRDG010000000
VERSION     DASRDG010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214329"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VFA35_10865"
     CDS             complement(<1..1180)
                     /locus_tag="VFA35_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZT56720.1"
                     /translation="MALDPHLTRDYAVDRRLLTIAALAVPIGALGTAAAWGLLTLIHL
                     FTNLFFFQTVSIVERAPASNTLGAWVIVLPVIGGLVVGLMARFGSEKIRGHGIPEAIE
                     AILFGKSRMSAKVALLKPLSSGIVIGSGGPFGAEGPIIMTGGALGSLLGQLFHLTAGE
                     RKTLLVAGATAGMTAVFGTPVAAVLLAVELLLFELRPRSLLPVAVACAVAGFTRPLLL
                     DAGRLFPLHTEAADGAALAACVLAGLLAGLLSSALSRALYATEDLFHRLPLHWMWWPA
                     IGGLAVGIGGWLQPRALGVGYDVIGDLLNHRLLVGAALALLAVKALMWLVALGSGTSG
                     GVLAPLLMIGAGLGTVMSVWLPVGDVSLWPLVCMAAVLAGVLGAPLTATVFAFGLTGD
                     A"
     gene            complement(1193..>2024)
                     /locus_tag="VFA35_10870"
     CDS             complement(1193..>2024)
                     /locus_tag="VFA35_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZT56721.1"
                     /translation="FALFIAAALSITAFPVMARILKDRGLTNAPFGQLALSAAAMVDV
                     FAWVLLAGVVAMAGAGEGDAALLKTAGGVLLLVAVVYGALRPGFAWLLRTHAPDGEPT
                     TGVLTALMIGLLVCALATEWLQLHAVFGAFLFGTCLPRDDRLLRSLGQRIEPLSMVVL
                     MPLFFALAGLGTTARAFGSASLGAMALIVAVAVVGKLAGAALGARIAGYGWRDSLCTG
                     ALMNARGLMELIVMKVGLDAGLIGPELFTMLLVMALVTTAMTGPLMTLFAGRGIYVTT
                     "
BASE COUNT          369 a          760 c          695 g          200 t
ORIGIN      
        1 tggcgtcgcc ggtcaggccg aacgcgaaca cggttgcggt cagcggcgcg ccgagcacgc
       61 cggccagcac cgcggccatg cacaccagcg gccagagcga gacgtcgccg accgggagcc
      121 acaccgacat caccgtgccg agcccggcgc cgatcatcag cagcggcgcg agcacgccgc
      181 ccgaggtgcc cgaaccgagc gcgacgagcc acatcagcgc cttcaccgcg agcagcgcga
      241 gtgccgcgcc gacgagcaga cgatggttca gcaggtcgcc gatcacgtcg tagccgaccc
      301 cgagcgcgcg cggctgcagc cagccgccga tgccgaccgc gagcccgccg atcgccggcc
      361 accacatcca gtgcagcggc aggcggtgaa acaggtcttc ggtcgcatac agcgcgcgcg
      421 acagcgccga cgacaacagc cccgcaagca ggcccgcaag cacgcaggcg gccagcgcag
      481 ccccgtcggc ggcctcggtg tgcagcggga acaggcggcc ggcatcgagc agcagcgggc
      541 gcgtgaagcc ggcgacggcg caggcgaccg ccacgggcag caggctgcgc ggacgcaact
      601 cgaacagcag cagttcgacc gcgagcagca ccgccgcgac cggcgtgccg aacacggccg
      661 tcatcccggc ggtcgcgccg gcgacgagca gcgtcttgcg ctcgccggcg gtgaggtgga
      721 acaactggcc gagcagggag ccgagcgcgc cgccggtcat gatgatcggc ccttcggccc
      781 cgaacgggcc acccgagccg atcacgatgc ccgaggacag cggcttgagc aacgcgacct
      841 tcgccgacat ccggctcttg ccgaacagga tcgcctcgat cgcctcgggg atgccgtggc
      901 cgcggatctt ctcggagccg aagcgcgcca tcagcccgac gaccaggccg ccgatcaccg
      961 gcagcacgat gacccaggcg cccagcgtgt tcgaggccgg cgcacgctcg acgatcgaga
     1021 cggtctgaaa gaagaacagg ttcgtgaaca ggtggatcag cgtcagcagc ccccacgccg
     1081 cggcggtgcc gagtgcgccg atcggcaccg ccagcgcggc gatcgtcagc aggcgccggt
     1141 cgacggcgta atcgcgcgtg aggtgggggt cgagggccat gccccaatta catcacgtcg
     1201 tgacataaat accgcggccc gcgaacagcg tcatcagcgg gccggtcatc gcggtggtca
     1261 cgagcgccat cacgagcagc atcgtgaaca gctccgggcc gatcagtccg gcgtcgagac
     1321 cgaccttcat cacgatcagt tccatcaggc cgcgcgcgtt catcagcgcg ccggtgcaga
     1381 ggctgtcgcg ccagccatag ccggcgatgc gcgcgccgag tgccgcgccg gccagcttgc
     1441 cgaccaccgc gaccgcgacg atcagcgcca tcgcgcccag gctcgcgctg ccgaaggcgc
     1501 gggccgtcgt gcccaggccg gccagcgcga agaacagcgg catcagcacg accatcgaca
     1561 gcggctcgat gcgctggccg agcgagcgca gcagccggtc gtcgcgcggc aggcaggtgc
     1621 cgaacaggaa cgcgccgaac accgcgtgca gttgcagcca ctcggtcgcc agcgcgcaca
     1681 ccagcaggcc gatcatcagc gcggtcagca cgccggtcgt cggctcgccg tcgggcgcgt
     1741 gcgtgcgcag cagccacgcg aacccggggc gcagcgcgcc gtagacgacc gcgaccagca
     1801 gcagcacgcc gccggcggtc ttcagcagcg ccgcatcccc ctcgcccgca cccgccatcg
     1861 caactactcc cgcaagcagc acccacgcga acacgtcgac catcgccgcg gcgctgagcg
     1921 cgagctggcc gaacggcgcg ttcgtcaagc cgcggtcctt caggatgcgc gccatgaccg
     1981 gaaacgcggt gatcgacagc gccgccgcga tgaacagcgc gaac
//
LOCUS       DASRDG010000649         3883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1705960, whole genome shotgun sequence.
ACCESSION   DASRDG010000649 DASRDG010000000
VERSION     DASRDG010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705960"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..620)
                     /locus_tag="VFA35_10875"
     CDS             complement(165..620)
                     /locus_tag="VFA35_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZT56722.1"
                     /translation="MPGPQPAPPAVVLDTNAVLDWLVFRNPELESLGAAIVRGDVRWI
                     ATAWMRAELAHVLDRGHLDAWQPELPGIWAQWERHCAQQPTPTFTGPSGRLRCSDPDD
                     QPFIDLARAGGARWLISRDRAVLKLARRLREHGVAVIPPGAWRPLDPTA"
     gene            777..1154
                     /gene="rpsL"
                     /locus_tag="VFA35_10880"
     CDS             777..1154
                     /gene="rpsL"
                     /locus_tag="VFA35_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZT56723.1"
                     /translation="MPTINQLVRHGRETEITKSKSPALQGGPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEIISYIGGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGSL
                     DLQGVKDRKQSRSKYGAKRPKKA"
     gene            1272..1742
                     /gene="rpsG"
                     /locus_tag="VFA35_10885"
     CDS             1272..1742
                     /gene="rpsG"
                     /locus_tag="VFA35_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZT56724.1"
                     /translation="MPRRREVPKRDILPDPKFGSVDLSKFMNVIMESGKKAIAERIIY
                     GALEQVEKKSGKDPLEVFVTALNNIKPMVEVKSRRVGGANYQVPVEVRPVRRVALAMR
                     WLKESARKRGEKSMAQRLANELLEAVEGRGGAMKKRDEVHRMAEANKAFSHFRF"
     gene            1821..>3883
                     /gene="fusA"
                     /locus_tag="VFA35_10890"
     CDS             1821..>3883
                     /gene="fusA"
                     /locus_tag="VFA35_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZT56725.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWKGMELGFPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRLAFVNKMDRTGANFYKVYEQMKV
                     RLKANPVPIVIPIGAEENFTGVIDLIKMKAIIWDEASQGMKFDYREIPAELLEEAKKW
                     RENMLEAAAESSEEMMNKYLETGDLTEDEIKLAIRTRTIAAEIQPMLCGSAFKNKGVQ
                     RMLDAVIDFMPSPIDVPPVPGHDDDDKEVVRRADDGEKFAALAFKLMTDPYVGQLTFV
                     RVYSGVLKSGDSVYNPIRGKKERIGRILQMHANQREEIKEILAGDIAACVGLKDVTTG
                     ETLCDPEAIITLEKMIFPEPVISQAVEPKTKADQEKMGIALGRLAQEDPSFRVKTDEE
                     SGQTIISGMGELHLEIIVDRMKREFGVEANVGKPQVAYRETIRKAVSDVEGKFVRQSG
                     GKGQYGHVVLKIEPQEPGKGFEFVDAIKGGVVPREFIPAVRKGVEDSLPAGVLAGYPV
                     VDVKVTLTFGSYHEVDSNENAFKMAASLGFKDGCRKAGPVILEPMMAVEVETPEDFMG
                     NVVGDLSSRRGMIQGMDDNPTGKVIKAEVPLAEMFGYSTTLRSLSQGRATYTMEFKHY
                     TEAPKS"
BASE COUNT          777 a         1216 c         1297 g          593 t
ORIGIN      
        1 gacgccgaac gcaaggccat cgtgatgcag gaggccgagg aactggccgc cgtgcaactc
       61 gcggccgtgg atgcggccac cgacgctgcg gccgccgaat aagcggcgcg caacgcaagc
      121 ttcaagggcg acttcggtcg ccctttttca tgggcgtgac cgcctcaggc tgtcgggtcg
      181 agcggccgcc aggcgcccgg cggaatcacc gcgacgccgt gctcgcgcag gcgccgcgcc
      241 agcttcagca cggcccggtc gcggctgatc agccagcgcg cgccgccggc acgcgccagg
      301 tcgatgaagg gctggtcgtc cgggtcgctg cagcgcagcc gcccagatgg gccggtgaac
      361 gtgggcgtgg gctgctgcgc gcagtggcgc tcccattgcg cccagatccc cggcaactcg
      421 ggttgccaag cgtcgaggtg acctcggtcg agcacgtgag ccagctccgc gcgcatccac
      481 gcggtggcga tccagcgcac atcgccgcgg acgatcgcgg caccgagcga ctcaagttcc
      541 ggattgcgga agaccaacca gtcgagcacg gcgttggtgt cgagcacgac ggcgggcggg
      601 gcgggttgcg gtccgggcat ggcggcattg tcgtctcgcg ccaagggctg gtcttgcgag
      661 gtctgccgga gcagcataca atcgagggct tttcggcaga cactgctgcg ctctttgctg
      721 cgccaagttg gcttgttcgc cggaaaccag tgacttcaaa cgggaacaag ttaccaatgc
      781 ctaccatcaa ccagctcgtg cgtcacggtc gcgagaccga gatcacgaag tccaagtcgc
      841 cggcgctgca aggcggcccg cagcggcgcg gcgtgtgcac gcgcgtgtac accacgacgc
      901 cgaagaagcc gaactctgcg cttcgtaaag tcgccaaggt tcgcctgacc aacggcttcg
      961 agatcatctc ctacatcggc ggcgaaggcc acaacctgca agagcacagc gttgtgctgg
     1021 tgcgtggcgg ccgtgtgaag gatctgccgg gtgttcgcta ccacattgtg cgcggctcgc
     1081 tcgacctgca aggcgtgaaa gaccgcaagc agtcgcgttc caagtacggc gccaagcggc
     1141 ccaagaaggc ttaaaccctt cgtccccatg cggcaggcca atcccggacc tgccgagtaa
     1201 gtggatggcc acagcgtgcc gtccgcggcg ggtgcgaaag cactgcgcca actgaagcca
     1261 aaggaagaga aatgccacgt cgtcgcgaag tccccaaacg ggacatcctc cctgacccga
     1321 agttcggttc ggtcgatctg tccaagttca tgaacgtgat catggaatcg ggcaagaagg
     1381 ccattgccga gcgcatcatc tacggtgcgc tcgagcaggt cgagaagaag tcgggcaagg
     1441 acccgctcga agtcttcgtc accgcgctga acaacatcaa gccgatggtc gaagtgaagt
     1501 cacgccgcgt cggcggtgcg aactaccagg ttcccgtgga agttcgcccg gtgcgccgcg
     1561 tggcgctggc gatgcgctgg ctgaaggagt cggcccgcaa gcgcggtgag aagtcgatgg
     1621 cgcagcgcct cgcgaacgag ttgctggagg ccgtcgaagg ccgcggcggc gcgatgaaga
     1681 agcgcgacga agtgcaccgc atggcagaag ccaacaaggc gttctcgcac ttccgcttct
     1741 aagacactgt ttttccccgt cccagccgct cctcgggttt gtacgaaccc ggtggcggct
     1801 cacgcatttg gagtgccatc atggcccgca agacccccat cgagcgctat cgcaacatcg
     1861 gcatctcggc gcacatcgac gccggcaaga ccacgaccac cgagcgcatc ctgttctaca
     1921 ccggcgtgaa ccacaagatc ggtgaagtgc acgacggtgc ggcgaccatg gactggatgg
     1981 agcaggagca ggagcgtggc atcacgatca cgtcggccgc gaccacctgc ttctggaagg
     2041 gcatggagct gggctttccc gagcaccgca tcaacatcat cgacaccccg gggcacgtcg
     2101 acttcacgat cgaggtcgag cgctcgatgc gcgtgctcga cggcgcgtgc atggtgtact
     2161 gcgcggtcgg cggcgtgcag ccgcagtccg agaccgtctg gcgccaggcc aacaagtaca
     2221 aggtgccgcg cctcgcgttc gtcaacaaga tggaccgcac cggcgcgaac ttctacaagg
     2281 tctacgagca gatgaaggtg cgcctgaagg cgaaccccgt gccgatcgtg atcccgatcg
     2341 gcgccgaaga aaacttcacc ggcgtgatcg acctgatcaa gatgaaggcg atcatctggg
     2401 acgaagcctc gcagggcatg aagttcgact accgcgagat ccccgccgag ctgctcgaag
     2461 aagccaagaa gtggcgcgag aacatgctcg aagccgcggc cgagtcgtcc gaagagatga
     2521 tgaacaagta cctcgaaacg ggtgacctga ccgaggacga gatcaagctc gccatccgca
     2581 cgcgcacgat cgcagccgaa atccagccga tgctgtgcgg cagcgcgttc aagaacaagg
     2641 gcgtgcagcg catgctcgac gccgtgatcg acttcatgcc ttcgcctatc gacgtgccgc
     2701 cggttcccgg ccacgacgac gacgacaagg aagtcgtgcg ccgtgccgat gacggcgaga
     2761 agttcgccgc gctcgcgttc aagctgatga ccgaccccta tgtcggccag ctgaccttcg
     2821 tgcgcgtcta ttcgggcgtg ctgaagtcgg gcgactcggt ctacaacccg atccgcggca
     2881 agaaggagcg catcggccgg atcctgcaga tgcacgcgaa ccagcgtgag gaaatcaagg
     2941 aaatcctggc cggcgacatc gctgcctgtg tgggcctgaa ggacgtgacg accggcgaga
     3001 cgctgtgcga cccggaggcg atcatcacgc ttgagaagat gatcttcccg gagccggtga
     3061 tttcgcaggc cgtcgagccc aagaccaagg ccgaccagga gaagatgggc atcgcgctgg
     3121 gtcgcctggc gcaggaggac ccgtcgttcc gcgtcaagac cgacgaagag tcgggccaga
     3181 ccatcatctc gggcatgggc gagctgcacc tggaaatcat cgtcgaccgc atgaagcgcg
     3241 agttcggcgt cgaggccaac gtcggcaaac cgcaggtcgc gtaccgcgaa acgattcgca
     3301 aggcggtgtc ggatgtcgaa ggcaagttcg tgcgccagtc ggggggcaag ggccagtacg
     3361 gccacgtcgt gctgaagatc gaaccgcagg agccgggcaa gggcttcgag ttcgtcgacg
     3421 cgatcaaggg cggcgtggtg ccgcgcgagt tcattcccgc ggtcaggaag ggcgtggaag
     3481 actcgctgcc ggccggcgtg ctggcgggct acccggtggt cgacgtgaag gtcacgctga
     3541 ccttcggttc gtaccacgag gtcgactcga acgaaaacgc gttcaagatg gctgcgtcgc
     3601 tcggtttcaa ggacggctgc cgcaaggccg gcccggtgat tctcgagccg atgatggcgg
     3661 tcgaagtcga gacgcccgaa gacttcatgg gcaacgtggt cggcgacctg tcgtcgcgcc
     3721 gcggcatgat ccagggcatg gacgacaacc cgaccggcaa ggtgatcaag gccgaagtgc
     3781 cgctggcgga gatgttcggc tactcgacga cgctccgctc gctgtcgcag ggccgcgcga
     3841 catacacgat ggagttcaag cactacaccg aggcgcccaa gtc
//
LOCUS       DASRDG010000650         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1706121, whole genome shotgun sequence.
ACCESSION   DASRDG010000650 DASRDG010000000
VERSION     DASRDG010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706121"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /locus_tag="VFA35_10895"
     CDS             <1..2025
                     /locus_tag="VFA35_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56726.1"
                     /translation="LEDQGPSMIPVNEFWRDYAVDPAGTPFRSTHFAAVTSSFAEMML
                     ALAVLDLPFTGAAPRPQREGNRLTMRPATPMLLVRQELLPAQPAAGQTEVLLSQNFYR
                     LDDRYRFENGERLDRFVTGEFLAGIVYGCQVVLTNPTSTPRKLDLLLQIPVGAVPVQS
                     GFQTRGIPVHLDAYATQKIEYAFYFPAPGDFARYPAHVSKDGAVVAAASVQPPVLHVV
                     AVPTEVDAGSWERVSQDATADDVLAFLGKANLQRLDLGRMLWRLHDRSFFERAVALLQ
                     SRHVYHDGVWSYAVLQNAEALTAQYLRHADAFVQRCGSALQSKLLTIEPVERGLWQQI
                     EFAPLFLSRAHQIGKRREILDRDLAAQYRALLDVLDHEPRLSAGDWLIATYYLLLQDR
                     VDDAVAAFARVDRSACPTHLQYDYLACYLDFYSDEPGRARAIAQPYADHPVERWRNLF
                     RSVLAQLDEAEGKATPGGDLQSRETRQGDLAASEPMLDFAVEGGKAIVRHKNLARCEV
                     RYYRMDVEFSFSTNPFVQQGSGSFAWVKPQRADALELKADAGETAFALPAEFQNQNVL
                     VELRGGGLVRRQTAFATSLRLQTIESYGQLQVTDAASKKPLPKVYVKVYARLPGGAVR
                     FHKDGYTDLRGRFDYASVTERTTAGADRYAILVLSEQNGAVIREVTPPAQ"
     gene            2151..>2478
                     /locus_tag="VFA35_10900"
     CDS             2151..>2478
                     /locus_tag="VFA35_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56727.1"
                     /translation="MRSLHLVAAAALLAAPVQSQAIIAQSSGLSNPDAVIDFGANLFP
                     NFTPVTTQFAGVVLTHARYFTTGVSNNLVGGFITNDPAIGNPNTLRIQFVQPITDVSF
                     VYHQIGQ"
BASE COUNT          372 a          931 c          827 g          348 t
ORIGIN      
        1 ctcgaggacc agggcccctc gatgattccc gtcaacgaat tctggcgcga ctacgccgtc
       61 gatccggcgg gcacgccgtt ccgctcgacg cacttcgccg ccgtcaccag cagcttcgcc
      121 gagatgatgc tggcgctcgc ggtgctcgac ctgccgttca ccggcgcggc gccgcgcccg
      181 cagcgcgaag gcaaccgcct caccatgcgc ccggcgacgc cgatgctgct cgtgcgccag
      241 gagctgctgc cggcgcagcc cgcggcgggc cagaccgagg tgctgctgag ccagaacttc
      301 taccggctcg acgaccgcta ccggttcgag aacggcgagc gcctggaccg cttcgtcacc
      361 ggcgagttcc tcgccggcat cgtctacggc tgccaggtgg tgctcaccaa cccgacgtcg
      421 acgccgcgca agctcgacct gctgctgcag atcccggtcg gcgccgtgcc cgtgcagtcc
      481 ggcttccaga cgcgcggcat cccggtgcac ctcgacgcct acgcgacgca gaagatcgag
      541 tacgcgttct acttcccggc gcccggcgac ttcgcgcgct acccggcgca cgtgagcaag
      601 gacggcgccg tggtcgccgc cgcgtcggtg cagccgcccg tgctgcacgt cgtcgcggtg
      661 ccgaccgagg tcgacgcggg ctcgtgggag cgcgtgtcgc aggacgcgac ggcagacgac
      721 gtgctcgcgt tcctcggcaa ggccaacctg cagcggctcg acctcggccg catgctgtgg
      781 cgtctgcacg accggtcgtt cttcgagcgc gcggtcgcgc tgctgcagag caggcacgtc
      841 taccacgacg gcgtctggtc ctacgcggtg ctgcagaacg ccgaggcgct gaccgcgcag
      901 tacctgcgcc acgccgacgc gttcgtgcag cgctgcggca gcgcgctgca gagcaagctg
      961 ctgaccatcg aaccggtcga gcgcggactc tggcagcaga tcgagttcgc gccgctgttc
     1021 ctgtcgcgcg cgcaccagat cggcaagcgg cgcgagatcc tcgaccgcga cctcgccgcg
     1081 cagtaccgcg cgctgctcga cgtgctcgac cacgagccgc ggctgtcggc cggcgactgg
     1141 ctgatcgcca cctactacct gctgctgcag gaccgcgtcg acgacgcagt ggcggcgttc
     1201 gcgcgcgtcg accgcagcgc gtgcccgacg cacctgcagt acgactacct cgcctgctac
     1261 ctcgacttct actccgacga gccgggccgc gcccgcgcga tcgcgcagcc gtacgcggac
     1321 cacccggtcg agcgctggcg caacctgttc cgcagcgtgc tcgcgcagct cgacgaggcc
     1381 gagggcaagg cgacgccggg cggcgacctg cagagccgcg agacgcggca gggcgacctc
     1441 gccgcgagcg agccgatgct cgacttcgcc gtcgagggcg gcaaggcgat cgtgcggcac
     1501 aagaacctcg cgcgctgcga agtgcgctac taccgcatgg acgtcgagtt ctcgttctcg
     1561 accaacccgt tcgtgcagca gggcagcggc agcttcgcgt gggtcaagcc gcagcgcgcc
     1621 gacgcgctgg agctgaaggc cgacgccggc gagaccgcgt tcgcgctgcc ggccgagttc
     1681 cagaaccaga acgtgctcgt cgagctgcgc ggcggcggcc tcgtgcgccg gcagaccgca
     1741 ttcgcgacct cgctgcggct gcagacgatc gagagctacg gccagctgca ggtcaccgac
     1801 gccgcgagca agaagccgct gccgaaggtc tacgtgaagg tctacgcgcg cctgcccggc
     1861 ggcgcggtgc gcttccacaa ggacggctac accgacctgc gcggccgctt cgactacgcg
     1921 tccgtgaccg agcgcacgac ggcaggggcc gaccgctacg cgatcctcgt gctcagcgag
     1981 cagaacgggg cggtgatccg cgaagtgacg ccgccggccc aatgatcgac ggcccgagga
     2041 ccggcgcgcc gcggttgcga aactggtgca gcacagcggc gcccgcggct ccgccggacg
     2101 ccgcgggcgg gtagtgttgc gtgcccattc gcttcgccaa ggagcctcgc atgcgttccc
     2161 tccacctcgt cgccgccgcg gcgctgctcg cggcgccggt ccagagccag gcgatcatcg
     2221 cccagtcgtc cggcctgtcg aatccggacg ccgtgatcga cttcggcgcc aacctgttcc
     2281 cgaacttcac gcccgtcacc acccagttcg ccggcgttgt gctcacgcac gcgcgctact
     2341 tcacgaccgg cgtgtcgaac aacctcgtcg gcggattcat caccaacgac ccggcgatcg
     2401 gcaaccccaa cacgctgcgc atccagttcg tgcagccgat caccgacgtc tcgttcgtct
     2461 accaccagat cgggcaga
//
LOCUS       DASRDG010000651         5614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1253269, whole genome shotgun sequence.
ACCESSION   DASRDG010000651 DASRDG010000000
VERSION     DASRDG010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253269"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /gene="dnaJ"
                     /locus_tag="VFA35_10905"
     CDS             <1..1100
                     /gene="dnaJ"
                     /locus_tag="VFA35_10905"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZT56728.1"
                     /translation="ASEEDIKKAYRKLAMKHHPDRNQGDDAKKSEGKFKEAKEAYEML
                     TDPHKRAAYDQYGHAGVDPNMGRGGPGAEGFGGFAEAFGDIFGDIFGGAAGGQRRGGG
                     QQVFRGSDLSYAMEITLEEAAHGKETQIRIPSYETCETCNGSGAKPGTSPKVCSTCNG
                     QGTVHMRQGFFSIQQTCPHCHGSGKIIPEPCTTCSGQGRIKKNKTLEVKIPAGINEGM
                     RIRSSGNGEPGTNGGPAGDLYIEIRIKQHEVFERDGDDLHCTVPVGLTTAALGGEIEV
                     PTLDGKAEIDLPEGTQHGKTFRLRGKGIKGVRSSYPGDLYCHVSVETPIKLTEHQRKL
                     LKELDEAFKKSGDRHSPNAKTWTDRVKDLFM"
     gene            1157..2830
                     /locus_tag="VFA35_10910"
     CDS             1157..2830
                     /locus_tag="VFA35_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HZT56729.1"
                     /translation="MTMRRTFSGLSGNPHADGWNPLTLALLAGVWIAALPNWPLWQAL
                     LALPETHTARGGLFVAGFGVMVAALTALLLTLLAWRATIKPAIALLLLAAAFGAHFMG
                     SYGVVIDTTMMTNVLQTDPREVRDLLSLRMLGTVLLLAGLPLLALWRVPVRGLGFWRQ
                     AGRNLLGLVAFALVLAALLFALFADLSATMRNHRTMRYLINPVNSLYALVDLGFASGR
                     VPSGPPLAIGADAHILPRPAGTKPTLLVLVVGETARADHFALDGYARPTNPALAALGV
                     ASFRNVMSCGTNTATSLPCMFSALGKKGFESRARDQQNLLDLVQRAGMAVLWVDNQAG
                     CKGLCERVPNAYAHDPVAGVAALPAALCDGEDCLDEALLHGLDARLAALPAAARARGV
                     LIVLHQMGSHGPAYFKRSPPDRKPFLPECTSNALQQCDHQALVNAYDNSIAYTDHVLG
                     QTIQWLQQQASRYDPALLYLSDHGESLGENNLYLHGLPYAVAPVEQKHVPMVLWLAAP
                     AAAQSACIAGLRDAALTHDNLFHSVLGLLGIRAAEYRPTLDAFAACRAP"
     gene            complement(2842..3981)
                     /locus_tag="VFA35_10915"
     CDS             complement(2842..3981)
                     /locus_tag="VFA35_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HZT56730.1"
                     /translation="MQTGRKIDGAQRDEPAAAARGPVKGRGTVWAIEHRFSPHTSEPC
                     DDGWGTLDQQASEERLAPATTIIEERVKSVLSRNDSPDISFDLSVNPYRGCEHGCVYC
                     FARPTHSYLNMSPGLDFETRIIAKVNAAEKLREAFASRRYEPRLLNIGAATDAYQPVE
                     RKLRITRAVIEVLAECAHPFSLVSKSSGVERDLDLIAPMAQRGLAAVYISVTSLDAAL
                     ARILEPRAAAPQRRLQTIAALARAGVPVGVSVSPIIPFINEPELERILEAAAEAGATS
                     AFSIVLRLPWEVNPLFQQWLDDHFPQRAARVMARVREMRGGHDYDARFGARMKGEGVW
                     AQLLDQRFRKAAARLGLNRQRVELDLTQFKRPSMPRADGQIDLFG"
     gene            4089..4550
                     /locus_tag="VFA35_10920"
     CDS             4089..4550
                     /locus_tag="VFA35_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56731.1"
                     /translation="MSIQHIHHGSTQGYQVRVGPRHAAMTKFFAVRKYGGKRKALAQA
                     KATELELRKIAPPTMPREGARVVAQANNTSGVIGVRPRYVLFSEHPYLYFVASWCVNG
                     KACSTSFSAERHGRLAALTLAMERRAKATGVKYDITPRQALNRMKHLLSAR"
     gene            4635..5099
                     /locus_tag="VFA35_10925"
     CDS             4635..5099
                     /locus_tag="VFA35_10925"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HZT56732.1"
                     /translation="MQALVYAVAVCAALALSVAAWRALRRHRPHASSPGFGDMRERDF
                     EALVAAALRAQGYEPIGATQGAPETITGEFMMRRERTSFLVTCRHFRIGRVDADAIRS
                     LQRAMAARGASAGVVLTSGRFSREAIAVAAGYGIRLVDGRALPTLLASVKTP"
     gene            5310..>5614
                     /locus_tag="VFA35_10930"
     CDS             5310..>5614
                     /locus_tag="VFA35_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HZT56733.1"
                     /translation="MHEFGGDFTVPKKAAELSPLAVNRITDAGLHFVGGVAGLALQVL
                     PSGGRTWILRLTVAGKRRDMGLGGFPDVTLAGARDAARAARSKADAGIDPIDERDSAR
                     "
BASE COUNT          882 a         1964 c         1919 g          849 t
ORIGIN      
        1 acgcctccga ggaagacatc aagaaggcct atcgcaaatt ggccatgaag caccaccctg
       61 accgcaacca gggtgacgac gcgaagaagt cggaaggcaa gttcaaggag gccaaggagg
      121 cctacgagat gctcaccgac ccgcacaagc gcgctgccta cgaccagtac ggccatgccg
      181 gtgtcgaccc gaacatgggc cgcggcggcc cgggcgcgga gggcttcggc ggctttgccg
      241 aggcgttcgg cgatatcttc ggcgacatct tcggcggcgc cgccggcggc cagcgccgcg
      301 gcggcggcca gcaggtgttc cgcggcagcg acctgagcta cgcgatggag atcacgctcg
      361 aagaagccgc gcacggcaag gagacgcaga tccgcatccc ctcgtacgag acctgcgaaa
      421 cctgcaatgg cagcggcgcg aagccgggca cgagcccgaa ggtctgcagc acctgcaacg
      481 gccagggcac ggtgcacatg cgccagggct tcttcagcat ccagcagacc tgcccgcact
      541 gccacggcag cggcaagatc atccccgagc cctgcaccac ctgcagcggc cagggccgga
      601 tcaagaagaa caagacgctg gaggtgaaga tccccgccgg catcaacgaa ggcatgcgca
      661 tccgctccag cggcaacggc gagccgggca cgaacggcgg gccggccggt gacctctaca
      721 tcgagatccg catcaagcag cacgaggtct tcgagcgcga cggcgacgac ctgcactgca
      781 cggtgccggt cggcctgacg accgcagcac tcggcggtga gatcgaggtg cccacgctcg
      841 acggcaaggc cgagatcgac ttgcccgaag gcacgcagca cggcaagacc ttccgcctgc
      901 gcggcaaggg catcaagggc gtgcgctcga gctacccggg cgacctctac tgtcatgtga
      961 gcgtcgagac gccgatcaag ctcaccgagc atcagcgcaa gctgctgaag gaactcgacg
     1021 aggcgttcaa gaagtcgggc gaccggcatt cgccgaacgc gaagacctgg accgaccggg
     1081 tcaaggatct gttcatgtga tcgacgcttc ggcgcccgcg ggcgccgaag tcgttcttgg
     1141 ccgccacact cgcccgatga ccatgcgcag gaccttctcc ggcctgtccg gcaacccgca
     1201 cgccgacggc tggaatccgc tgacgctcgc gctgctcgcc ggcgtgtgga tcgcggcact
     1261 gccgaactgg ccgctgtggc aagcgctgct cgcgctgccc gagacgcaca ccgcgcgcgg
     1321 cggcctcttc gttgccggct tcggcgtgat ggtcgcggcg ctgacggcgc tgctgctcac
     1381 cttgctcgcg tggcgcgcca cgatcaagcc ggcgatcgca ctgctactgc tcgccgccgc
     1441 gttcggcgcg cacttcatgg gcagctacgg cgtggtcatc gacacgacga tgatgaccaa
     1501 cgtgctgcag accgacccgc gcgaggttcg cgacctgctc agcctgcgca tgctgggcac
     1561 ggtgctgctg ctggccggcc tgcccttgct ggcgctgtgg cgcgtgccgg tgcgcggcct
     1621 cgggttctgg cggcaggcag ggcgcaatct gctcggcctc gtggcgttcg cgctggtgct
     1681 ggcggcgctg ctgttcgcgc tgttcgccga cctgtcggca acgatgcgca accaccgcac
     1741 gatgcgttac ctgatcaacc cggtgaattc gctctacgcg ctggtcgacc tcggcttcgc
     1801 gtcggggcgc gtgccgagcg ggccgccgct ggcgatcggc gccgacgcgc acatcctgcc
     1861 gcgcccggcg ggcacgaaac caacgctgct ggtgctggtg gtcggcgaga ccgcgcgcgc
     1921 cgatcacttc gcgctcgacg gctacgcgcg gccgaccaac ccggcgctgg cggcgctcgg
     1981 cgtggcgagc ttccgcaacg tgatgtcctg cggaaccaac accgccacct cgctgccctg
     2041 catgttctcg gcgctcggca agaagggatt cgagtcgcgc gcgcgcgatc agcagaacct
     2101 gctcgacctg gtccaacgcg ccggcatggc ggtgctgtgg gtcgacaacc aagccggctg
     2161 caagggcttg tgcgagcgcg tgccgaacgc ttatgcgcac gacccggtcg ccggcgtggc
     2221 ggccctgccg gccgcgctgt gcgatggtga agactgcctg gacgaagcgc tgctgcacgg
     2281 gctcgacgcc cgcctggccg ccctgcccgc cgccgcccgt gcccgcggcg tgctgatcgt
     2341 gctgcaccag atgggcagcc acgggcccgc ctacttcaag cgcagcccgc ccgaccgcaa
     2401 gccgttcttg cccgagtgca catcgaacgc gttgcagcag tgcgaccacc aggcgctggt
     2461 caacgcctac gacaactcga tcgcgtacac cgaccacgtg ctggggcaga cgattcagtg
     2521 gctgcagcag caggcatcgc gctacgaccc ggccctgctc tacctgtcgg accacggcga
     2581 gtcgctcggc gaaaacaacc tgtacctgca cggcctgccc tacgcggtgg cgccggtcga
     2641 gcagaagcat gtgccgatgg tgctgtggct cgcggcgccg gccgcggcgc agtcggcctg
     2701 catcgccggc ctgcgcgacg cggcgctgac gcacgacaac ctgttccatt cggtgctcgg
     2761 cctgctcggc atccgcgccg ccgagtaccg gccgaccctc gacgccttcg ccgcctgccg
     2821 cgcgccctga tcgcgcgacg ctcagccgaa caagtcgatt tgcccgtcgg cccgcggcat
     2881 cgaagggcgc ttgaactgcg tgaggtcgag ttcgacgcgt tggcggttca gcccgagccg
     2941 cgctgccgcc tttctgaagc gctgatcaag cagctgtgcc cacacgccct cgcctttcat
     3001 gcgtgcaccg aagcgcgcgt cgtagtcgtg cccgccgcgc atctcgcgca cgcgcgccat
     3061 cacgcgggcg gcgcgctgcg gaaaatgatc gtcgagccac tgctggaaca gcggattcac
     3121 ctcccagggc agccgcagca cgatgctgaa ggccgaggtc gcaccggcct cggccgcggc
     3181 ctcgaggatg cgctcgagtt cgggctcgtt gatgaaaggg atgatcggcg acacgctcac
     3241 cccgaccggc acgccagccc gcgcgagcgc cgcgatcgtc tgcagccggc gttgcggcgc
     3301 ggcggcgcgc ggttcgagga tgcgcgcgag cgcggcgtcg agcgaggtca ccgagatgta
     3361 gaccgccgcg aggccgcgct gcgccatcgg cgcaatcaga tcgagatcgc gctcgacgcc
     3421 cgacgacttg ctgaccagcg agaacgggtg cgcgcactcg gccagcacct cgatcaccgc
     3481 gcgcgtgatg cgcagcttgc gctcgaccgg ctgataggcg tcggtcgcgg cgccgatgtt
     3541 gagcagccgc ggctcgtagc ggcggctcgc gaaggcctcg cgcagcttct cggccgcgtt
     3601 gaccttggcg atgatgcggg tctcgaagtc gagcccgggc gacatgttca ggtagctgtg
     3661 cgtcggccgc gcgaagcaat agacgcaacc atgctcgcag ccgcggtatg ggttcaccga
     3721 caggtcgaac gagatgtcgg gcgagtcgtt gcgcgacagg accgatttga cgcgctcctc
     3781 gatgatcgtc gtggccggcg cgaggcgctc ctcgctggcc tgctggtcga gcgtgcccca
     3841 gccgtcgtcg cagggctcgc tcgtgtgcgg gctgaaccgg tgctcgatcg cccacaccgt
     3901 tccgcggccc ttcacggggc cgcgggccgc ggcagcgggt tcgtcgcgct gcgctccgtc
     3961 gatcttccgg cctgtttgca tactgtgaat atatccagct ttcggcacga tgccaacgcg
     4021 ccgcctggcg aacgcgacaa tgcagtcctc cgaagccgtc atctttccgg ccgaaaggtc
     4081 aatcccctat gtcgattcaa cacatccatc acggctccac ccagggctac caggtgcgtg
     4141 tcggcccgcg ccacgcggcg atgacgaagt tcttcgcggt gcgcaagtac ggcggcaagc
     4201 gcaaggcgct cgcgcaggcg aaggcgaccg aactcgaact gcgcaagatc gcgccgccga
     4261 cgatgccgcg cgagggtgcg cgcgtcgtgg cgcaggccaa caacaccagc ggtgtgatcg
     4321 gcgtgcggcc gcgctacgtg ctgttctcgg agcacccgta cctgtatttc gtcgcctcct
     4381 ggtgcgtcaa cggcaaggcc tgctcgacca gcttctcggc cgagcggcac ggcaggctgg
     4441 cggcgctgac gctggcgatg gagaggcgcg cgaaggcgac cggcgtcaag tacgacatca
     4501 cgccgagaca agcgctcaac cggatgaagc acctgctcag cgcgcgctga ggctgcggct
     4561 tccgtcacgg attgcgtgac ggcgtgcgca ccgcggcgcg ccgtcgccga agtgaacgcg
     4621 ctagcctgcg ggcgatgcaa gccctcgtct atgccgtggc ggtgtgtgcc gccctcgccc
     4681 tgtcggtcgc tgcctggcgg gcgctgcggc gccatcgccc gcacgcgtcg tcgccgggct
     4741 tcggcgacat gcgcgagcgt gacttcgaag cgctggtcgc ggcggccctg cgtgcccagg
     4801 gctacgagcc gatcggcgcg acgcagggcg ccccggagac gatcaccggc gagttcatga
     4861 tgcgacgcga gcgcacgagc tttctcgtca cgtgccggca tttccgcatc ggccgggtgg
     4921 atgccgacgc catccggtcg ctgcagcgcg cgatggccgc acgcggcgca tccgccggcg
     4981 tcgtgctgac cagcgggcgc ttcagccgcg aggcgatcgc agtggccgcg ggttacggca
     5041 tccggctcgt cgacggccgt gcgctgccga cgttgctcgc gagcgtgaag acaccgtaag
     5101 cgcgcgccgg catgacaaac gggcccctcg gggcccgttc tttctgattc ttcgccttgg
     5161 cggagtcgga gtcctccgga atcgaacttc gtgacgcatc acctcacttc acccgccggg
     5221 tgagacgagc ctcagccccg taatcgttgc atcctgtcgt atcgcgtcgt atcatccagt
     5281 ctcagaattg ttgatgggtg agaaaatggg tgcacgagtt cggcggagac ttcacagtgc
     5341 ctaagaaggc ggccgagctg agcccgctcg cggtgaatcg catcaccgat gccgggcttc
     5401 atttcgtcgg cggcgtggca gggctcgcgc tgcaggtatt gccgagcggc gggcgcacgt
     5461 ggattctgcg cctcactgtc gcgggcaagc gccgggacat ggggctcggt ggcttcccgg
     5521 acgtcacgct cgccggcgcg cgcgacgcgg cccgtgcggc ccgatcgaag gctgatgccg
     5581 gcatcgaccc catcgacgag cgcgattccg cccg
//
LOCUS       DASRDG010000652         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1253283, whole genome shotgun sequence.
ACCESSION   DASRDG010000652 DASRDG010000000
VERSION     DASRDG010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253283"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFA35_10935"
     CDS             <1..331
                     /locus_tag="VFA35_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56734.1"
                     /translation="HRLILTRSLRTALHTLLRVRAADANAPYKLIRREVALAALASMP
                     PAPRIPSVLLTVYLSRRAHRVTEQPVTHFARTAGQPSLRLRRLAVFCRGALSELMRFH
                     RALSQRT"
     gene            328..2409
                     /locus_tag="VFA35_10940"
     CDS             328..2409
                     /locus_tag="VFA35_10940"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HZT56735.1"
                     /translation="MSGGIALAWSDGSFVYALRWWATLLLLSAGGWLLAERGLRHWHD
                     RGWAISRPLFLLALAYPIWALANFFPVFTPAFQWSGAGCLLLAGLLLRFKTGVPAREA
                     FPLRTLAFAELRFLLPFAFYLAMRGFSHDLIGLEKFMDFAFVNSAERSATLPPPDPWF
                     AGAPINYYYFGHYLAAFLCKLTGVPPAFGYNLMLATLFGSVFQLSYALVMELTAGVPQ
                     TLRNAMSAITATWLTLGGNIHGFLYGFIKPWLVDAGWLARPAQAFLLSDPTRFVGHDP
                     PTQDKLIHEFPAYAFYVGDLHAHLSNLPQVLLLLAALLEWGRRSAQGAQKARFVWLAI
                     AAALVGIFAMANSWDALMYAGLLGVLLVLRGLAAVRHGARALGVAVSEGLLAGIVVAA
                     TALPFFLHFEPLSDGFHLTHSHTPAWQWMILYGEQAMLGLLGCALAFSDRVRRLQPES
                     RFLAMLVGFGIAFALVPELVYLKDIYGAEFYRGNTAFKFGFQAFTLLTVAAGAALAMI
                     AALPLWRGRTVALVLALELALVPPLYYGWFVLQGGLGVWREREWTLDGLRYVTLSYPE
                     DAAAIAWLRQQPRDVVVEAVGDSYTYAARIATNAGGPDVLGWPVHEQLWRGSSPQVWE
                     RRDDVAHFYTGRDADAARRFLARYHARWIVVGGFERERYRELDVAFLESLGRVVFRSG
                     NTFIVEVRPVQ"
     gene            complement(2381..>2711)
                     /locus_tag="VFA35_10945"
     CDS             complement(2381..>2711)
                     /locus_tag="VFA35_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56736.1"
                     /translation="IKANLDRYGEIRLTHAAFNGNETLIDFYLTGAEREAVKVRNAKA
                     PCVLDEARTQVWILREERHVRLEHSACPVVRQILQTLYDPRGAPLFRMVQISPLPEFI
                     APDEPRR"
BASE COUNT          368 a          930 c          841 g          572 t
ORIGIN      
        1 ccatcgcctg atcctgaccc gcagcctcag gactgcgctg catactctgt tgcgcgtgcg
       61 cgcggccgat gccaacgctc cgtacaaact gatacgtcgg gaagtggcgc tggccgcgtt
      121 ggccagcatg ccgccggcac cgcgcattcc gtccgtgctg ctcacggtct acctctcgcg
      181 ccgtgcgcat cgagtgacgg aacagccggt cacgcacttc gcccgaacgg caggacaacc
      241 gtccctgcgg ctgcgccgac tcgcggtgtt ctgtcgcggc gctttatccg aactgatgcg
      301 tttccatcgc gcactgtcgc aacggacatg agtggcggca tcgcgctcgc ctggagcgac
      361 ggctccttcg tctacgcact gcgatggtgg gccacgcttc tgctgctgag cgcgggcgga
      421 tggctgctcg ccgaacgcgg gctgcgccat tggcacgatc gcggctgggc catttcgcga
      481 ccgctgttcc tgctggcgct ggcttacccc atttgggccc tcgcgaattt ttttccggtg
      541 ttcacgccgg ccttccaatg gagcggcgca ggctgcctgc tgctggcggg tctgctcctg
      601 cgtttcaaga ccggtgttcc ggcccgtgaa gcgtttccgc tgcgcacgct tgccttcgcc
      661 gagctgcgct tcttgctgcc cttcgcgttc tacctcgcca tgcgcggctt cagccacgat
      721 ctcatcgggc tggaaaaatt catggacttc gccttcgtca attccgccga gcgcagcgcc
      781 accctgccgc ctcccgaccc ctggttcgcc ggcgcgccga tcaactacta ctacttcggc
      841 cactatctcg cggctttcct gtgcaagctc acgggcgtcc cacccgcgtt cggctacaac
      901 ctgatgctgg ccacgttgtt cggatccgtt ttccaactga gctacgcgct ggtgatggag
      961 ctcacggcgg gcgtgccgca gactctgcgc aacgccatgt cggcgatcac ggcaacctgg
     1021 ctcacgctcg gcggcaacat ccacggcttt ctctacggct tcatcaagcc ctggctcgtc
     1081 gacgccggtt ggctcgcgcg gccggcgcag gcgttcctgt tgtccgatcc cacgcgcttc
     1141 gtcggccacg acccgccgac ccaggacaag ctgatccacg agtttcccgc ctatgcattc
     1201 tacgtgggcg atctgcacgc gcacctttcc aatcttccgc aggtgctgtt gctcctggcc
     1261 gcgttgctcg aatgggggcg gcgcagcgct caaggtgcgc agaaggcgcg cttcgtctgg
     1321 ctcgccatcg cagccgccct ggtcggcatt tttgccatgg cgaacagctg ggacgcgctg
     1381 atgtacgcgg gactgctcgg cgtgctgctc gtgttgcgcg gcctcgctgc cgtacgccat
     1441 ggagcgcgcg cgctcggcgt cgcggtaagc gaaggcctgc tggccggcat cgtcgtcgcg
     1501 gcgaccgcgc tgcctttctt cctgcacttc gagccgcttt cggacggttt tcacctcacc
     1561 cacagccaca cgccggcgtg gcagtggatg atcctgtacg gcgagcaggc gatgctcggc
     1621 ctgttaggtt gcgcactcgc cttttcggac cgcgtgcgac ggcttcagcc cgagtcgcgc
     1681 ttcctcgcca tgctggtcgg gttcggcatc gccttcgcgc tggtcccgga actggtctac
     1741 ctgaaggaca tctatggcgc ggaattctat cgtggcaaca ccgcgttcaa attcggtttc
     1801 caggccttca cgctgctcac cgtcgccgcc ggcgccgccc ttgccatgat cgcagcgctt
     1861 ccgctgtggc gcggacgcac tgtagcgctg gttctggcac tcgagctcgc gctggtgcca
     1921 cccctgtact atggctggtt cgtcctgcag ggcggcctcg gcgtctggcg cgagcgtgag
     1981 tggaccttgg acgggctgcg ctacgtcacc ctgtcctatc cggaggacgc ggcggcgatt
     2041 gcctggttga gacagcagcc gcgcgacgtg gtcgtggagg cagtcggtga cagctatacc
     2101 tatgccgcgc gcatcgccac gaatgccggc gggcccgatg tgctgggctg gccggtgcac
     2161 gagcaactct ggcgcggcag cagcccgcaa gtatgggagc ggcgcgacga tgtcgcccac
     2221 ttctacacgg gccgcgatgc cgatgccgca cgccggtttc ttgcccgtta tcatgcacgc
     2281 tggatcgtcg tcggcgggtt cgagcgcgag cgctatcgcg agctcgacgt ggcttttctc
     2341 gaatcactgg ggcgggtcgt cttccgcagc ggcaacacct tcatcgtcga ggttcgtccg
     2401 gtgcaatgaa ttccggcagt ggcgatatct gcaccatgcg gaaaagcggt gcgcccctgg
     2461 ggtcgtacag cgtctggagt atctggcgga caaccgggca cgcactgtgc tcgagtctca
     2521 catgacgctc ctcccgcagg atccatactt gcgtgcgcgc ctcgtccagc acgcatggcg
     2581 ctttcgcgtt tctcaccttc accgcttcgc gttccgcacc ggtgagatag aaatcgatca
     2641 gcgtctcgtt gccgttgaac gctgcgtgtg tgagtcgtat ctccccatac cggtcgagat
     2701 tcgcttttat c
//
LOCUS       DASRDG010000653         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_214907, whole genome shotgun sequence.
ACCESSION   DASRDG010000653 DASRDG010000000
VERSION     DASRDG010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214907"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VFA35_10950"
     CDS             complement(<1..1745)
                     /locus_tag="VFA35_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZT56737.1"
                     /translation="MNPLDLEPARPARAASPTEGLAFLWRGRFTIALATLLGAGGGVF
                     YAQHRGTIYRARSVLYVERDSPMMPGSSVSLWLQSRNYANTQSALLRSTPILEKCLQN
                     PALFTSQLFGDSTNRLAWLGRHLSVAVGNEDDLITVSLDSVQIEDACTIVNSVVDAYL
                     SFHARNRRETTGEVLVGLQQQLASHETELAAIQKEQFEFLARHPTLVLSGEAGKIATE
                     RLRELNNAMTRAEIDTSEAEAAWQAAKTMAESNDALRRLPLLNGDPVLVQSAVGDVKR
                     VDELKQRRLALQQEVTPDHPDLLRLDGVIAALEKENAAHAHQLAQAAISALEARYRGA
                     KQRFDALASRIAEEEKRLQNVDPAEAEYRGIEIRFDRERKLTDSLYERIRSIDINEKV
                     DTSDKPQLSTLVYEYASPDSAPVASSKTAIAAIATFVGFVLGIAFAWFRALTDQRLRS
                     PDDVVAQLPLLGTLPRVAIDHRGVLHGWRRSPVYVAAMRSLRTVLQFNVRSARHRVLQ
                     VTSTRNGDGKSTVVAGLGIAMAMSGERTLIVDADFGRAAQARLFGLAGEVGLAQALAS
                     RSPAAQSIVATAVEKL"
     gene            complement(1748..>3326)
                     /locus_tag="VFA35_10955"
     CDS             complement(1748..>3326)
                     /locus_tag="VFA35_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZT56738.1"
                     /translation="GVAGIRCVRRLQRQHRRTVEESRRGPRGTPTVLIGAGVGGRLLL
                     QELAEHGDLGYQPVAFVDDDEEKAGRVVGGVRVLGTTADLPAIAARFSAKVALITIPS
                     AGGREINRIVDRCKAAGMQTKIVPHLYEIVSGRVRISQLRDVAIEDLLGRDPVVIENS
                     AAPAVVEGRVVMVTGAGGSIGSELCRQLLHLRPSRLLLVERTENSLYEIHRELTAGDS
                     GDRVVPELADVCDAERMTELFASHRPHLVFHAAAHKHVPMMERVPCEAIKNNVFGTKT
                     VADLADRFGVERFVFVSTDKAVRPASVMGATKRLAELYLKDLAARSRVRYASVRFGNV
                     MGSAGSVIPLFREQIARGGPVTVTHELMERFFMTIPEACRLVLEAAGLCEGGEVMLLD
                     MGKPVRIRELAENMIRLSGYEPNSQIPIAITGVRPGEKLHEELSLPEEMVDGTRCKKL
                     FVWRGGAAAQPMPAILERLRAPGHTPDEIRRTLHEVLPEYSSAEPAGSLLPTGLLPTG
                     LLPAVAGSPQRAGSMSA"
BASE COUNT          459 a         1186 c         1193 g          488 t
ORIGIN      
        1 agcttctcga ccgccgtcgc gacgatcgac tgggccgcgg gcgaacggct cgcgagcgcc
       61 tgcgcgaggc cgacctcgcc ggcgaggccg aacaggcgcg cctgcgccgc ccggccgaag
      121 tcggcgtcga cgatcagcgt gcgctcgccg gacatcgcca tcgcgatgcc gaggcccgcg
      181 accaccgtcg acttgccgtc gccgttgcgc gtcgacgtga cctgcagcac gcgatggcgg
      241 gcgctgcgga cgttgaactg cagcaccgtg cgcagcgacc gcatcgccgc cacgtacacg
      301 ggcgatcgcc gccagccgtg cagcacgccg cggtggtcga tcgcgacccg cggcagcgtc
      361 ccgagcagcg gcagctgcgc cacgacgtcg tcgggcgagc gcaggcgctg gtcggtgagc
      421 gcgcggaacc atgcgaaggc gatgccgagc acgaagccga cgaacgtggc gatcgccgcg
      481 atcgccgtct tcgacgacgc gaccggcgcg ctgtccggcg acgcgtactc gtagacgagc
      541 gtgctgagct gcggcttgtc cgacgtgtcg accttctcgt tgatgtcgat cgaacggatg
      601 cgctcgtaga gcgagtcggt gagcttgcgc tcgcggtcga aacggatctc gatgccgcgg
      661 tactccgcct cggcggggtc gacgttctgc aggcgcttct cctcctcggc gatgcgcgaa
      721 gcaagggcgt cgaagcgctg cttggcgccg cggtagcgcg cctcgagcgc gctgatcgcc
      781 gcctgcgcga gctgatgcgc gtgcgccgcg ttctccttct cgagcgcggc gatgacgccg
      841 tcgaggcgca agaggtcggg gtggtcgggc gtgacctcct gctgcagcgc cagccggcgc
      901 tgcttgagct cgtcgacgcg cttgacgtcg ccgaccgcgg actgcaccag cacgggatcg
      961 ccgttgagca gcggcagacg gcgcagcgcg tcgttcgatt cggccatcgt cttcgccgcc
     1021 tgccacgccg cctcggcctc gctcgtgtcg atctcggcgc gcgtcatggc gttgttgagc
     1081 tcgcgcaggc gctcggtggc gatcttgcct gcttcaccgg agagcacgag cgtcgggtgc
     1141 ctggccagga actcgaactg ctccttctgg atcgccgcca gctcggtctc gtggctggcg
     1201 agctgctgct gcaggccgac gagcacttcg cccgtggtct cgcggcggtt gcgcgcgtgg
     1261 aacgacaggt aggcgtcgac caccgaattg acgatggtgc acgcgtcctc gatctgcacc
     1321 gagtcgaggc tgacggtgat gaggtcgtcc tcgttgccga cggcgaccga caggtggcgc
     1381 ccgagccacg ccagccggtt cgtcgagtcg ccgaacagct ggctggtgaa gagcgccggg
     1441 ttctgcaggc acttctcgag gatcggcgtc gaacgcagca gggccgactg cgtgttggcg
     1501 tagttgcgcg actgcaacca cagcgacacg ctgctgccgg gcatcatcgg cgaatcgcgc
     1561 tcgacgtaga gcaccgagcg cgcgcggtag atcgtgccgc ggtgctgcgc gtagaacacg
     1621 ccgccgccgg cgccgagcag cgtcgcgagc gcgatggtga accggccgcg ccagaggaac
     1681 gccagcccct ccgtcggcga cgccgcgcgc gcggggcgcg ccggctcgag atcgagcggg
     1741 ttcatggcta cgccgacatg ctgcccgcgc gctgtggcga tccggccacc gccggcagca
     1801 acccagttgg cagcaaccct gttggcagca gcgaaccggc gggttcggcg gacgagtact
     1861 cggggagcac ctcgtgcagc gtgcggcgga tctcgtcggg cgtgtgcccc ggcgcgcgca
     1921 gccgctcgag gatcgccggc atcggctgcg cggcagcgcc gccgcgccag acgaacagct
     1981 tcttgcatcg ggtcccgtcg accatctcct cgggcagcga cagctcctcg tgcagcttct
     2041 cgccggggcg cacgcccgtg atcgcgatcg ggatctgcga gttcggctcg tagcccgaca
     2101 gccggatcat gttctcggcg agctccctga tgcgcacggg cttgcccatg tcgagcagca
     2161 tgacctcgcc gccctcgcag agccccgccg cctcgagcac gagccggcac gcctcgggga
     2221 tggtcatgaa gaaccgctcc atcagctcgt gcgtgacggt caccgggccg cccctggcga
     2281 tctgctcgcg gaacagcggg atcacgctgc ccgcggagcc catcacgttg ccgaagcgca
     2341 ccgacgcgta gcgcacgcgg ctgcgcgccg cgagatcctt gaggtagagc tcggcgagcc
     2401 gcttggtcgc gcccatcacc gacgcgggcc gcaccgcctt gtcggtcgac acgaacacga
     2461 agcgctcgac gccgaaccgg tccgcgaggt cggcgacggt cttggtgccg aacacgttgt
     2521 tcttgatcgc ctcgcacggc acgcgctcca tcatcggcac gtgcttgtgc gcggcggcgt
     2581 ggaacacgag gtgcggccgg tgcgacgcga acagctccgt catgcgctcg gcgtcgcaga
     2641 cgtcggccag ctcgggcacg acgcggtccc cggagtcgcc cgcggtgagc tcgcggtgga
     2701 tctcgtagag gctgttctcg gtgcgctcga ccagcagcag ccgcgacggc cgcaggtgca
     2761 gcagctggcg gcacagctcc gagccgatgc tgccgcccgc gcccgtgacc atgaccacgc
     2821 ggccctcgac gacggcgggc gcggcgctgt tctcgatcac cacggggtcg cggccgagca
     2881 ggtcctcgat cgcgacgtcg cgcagctgcg agatgcgcac cctgccgctc acgatctcgt
     2941 agaggtgcgg cacgatcttg gtctgcatcc ccgcagcctt gcagcggtcg acgatgcggt
     3001 tgatctcgcg gccgcccgcg ctcgggatcg tgatcagcgc gaccttggcg gagaagcgcg
     3061 cggcgatcgc cggcaggtcg gcggtggtgc cgagcacgcg gacgccgccg accacgcggc
     3121 cggccttctc ctcgtcgtcg tcgacgaacg ccaccggctg gtagccgaga tcgccgtgct
     3181 cggcgagctc ctgcagcagg aggcggccgc cgacgccggc gccgatcagc accgtcggcg
     3241 tgccccgcgg cccgcgccgc gactcctcga cggtgcggcg gtgctgcctc tggagccgcc
     3301 gtacgcagcg gatgccggcg acgccc
//
LOCUS       DASRDG010000654         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_555959, whole genome shotgun sequence.
ACCESSION   DASRDG010000654 DASRDG010000000
VERSION     DASRDG010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555959"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFA35_10960"
     CDS             <1..545
                     /locus_tag="VFA35_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZT56739.1"
                     /translation="TEAARLGGDPTRVFVMGHSAGAYNAAMLALDPRWLGATGHTPTE
                     LAGWIGLAGPYDFFPTDNLEARPVFFHPNYPPLAQPIEFAAAKGRPVFLGAPIDDAVV
                     DPTRSTRALAAQQQAAGTPVDLKMYARASHTTLIGAFATPLRWIAPVLDDVLAFIAAT
                     PATVQRPGSAVASNIAKVGG"
     gene            complement(494..793)
                     /locus_tag="VFA35_10965"
     CDS             complement(494..793)
                     /locus_tag="VFA35_10965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZT56740.1"
                     /translation="MIIVTGTLHARPDALAEVLRLSREHVQRSRAEPGCLEHGVAIDA
                     DDPLRLVFFERWADHDAIGAHLRLPASRGFGKAVAALVDQPPTLAMFDATALPGR"
     gene            complement(825..1580)
                     /locus_tag="VFA35_10970"
     CDS             complement(825..1580)
                     /locus_tag="VFA35_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZT56741.1"
                     /translation="MPALRLTWPLPALLAWALGWAVFALLRSLGAPLALAIAVPIALG
                     AALSALGTTPWRRVFVAAGFPLSLLASGLAGAMPAWGWLLPLALLALVYPLNAWHDAP
                     LFPTPAGALDGLARLAPLAPNARVLDAGCGLGAGLRELHREYPAAATHGLEWSWPLRI
                     ACAWGCRFATVRRGDIWAADWSGYDLVYVFQRPESMDRAAAKATRELRPGAWLVSLEF
                     DAPGFAPTKVIECADGRRLWLYRVPFRTRPALS"
     gene            complement(1591..>2548)
                     /locus_tag="VFA35_10975"
     CDS             complement(1591..>2548)
                     /locus_tag="VFA35_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZT56742.1"
                     /translation="TRLVAFNASVEAKRAGDAGRGFGVVADAVKDLAGKVEFSSKEIM
                     RTVGVLDRRIDTLAREIRFDANAAQQGEFHAALADVQAAVASIITAAEQSRSICGGLD
                     AQMSGIVTEMDRTSGVLGAAMKRSETFLDVSEHLIEIVAECGIETEDTPYIQAVMEGA
                     NQLGAILDAALAGGATTLDVLFDEVYRPIVGTNPPQHLTRFTPVTDKLFPQVLERMLE
                     VSPKVVFCMAGDRNGYVPTHNKKHNVPQRGDLVWDTANSRYRRIFNDRTGLASTRNRR
                     PFLLQTYRRDMGGGNFVVMKEAAAPITVKGKHWGGLRLAFRF"
BASE COUNT          375 a          931 c          906 g          336 t
ORIGIN      
        1 tgaccgaagc ggcgcgcctg ggcggcgatc cgacgcgcgt gttcgtgatg ggtcacagcg
       61 ccggcgccta caacgccgca atgctcgcgc tcgatccgcg ctggctgggc gccaccggcc
      121 acacgccgac cgaactcgcg ggctggatcg gcctggccgg cccctacgac ttcttcccca
      181 ccgacaacct cgaggcgcgg ccggtgttct tccacccgaa ctacccgccg ctggcgcagc
      241 cgatcgagtt cgccgcggcg aaagggcggc ccgtctttct cggcgcgccg atcgacgacg
      301 ccgtcgtcga cccgacccgc agcacgcgcg cactcgccgc ccagcagcag gccgccggaa
      361 cgccggtcga cctgaagatg tacgcgcgcg ccagccacac gacgctgatc ggcgcgttcg
      421 cgacaccgct gcgctggatc gcgccggtgc tcgacgacgt gctggcgttc atcgccgcca
      481 cgccggcgac tgttcagcgc cccggcagcg ccgtcgcgtc gaacatcgcc aaggtcggcg
      541 gctgatcgac cagcgcggca accgccttgc cgaagccgcg cgaggccggc agccgcaggt
      601 gcgcgccgat cgcgtcgtga tcggcccagc gttcgaagaa caccagccgc agcgggtcgt
      661 cggcatcgat cgcgacaccg tgctccaggc agccgggctc ggcgcgcgag cgctgcacgt
      721 gttcgcggct gaggcgcagc acctcggcca gcgcgtcggg tcgggcatgc aatgtgccgg
      781 tgacgatgat catcgtggtc tcctcgcggt ggggcaccgc gccctcacga tagcgcaggc
      841 cgggtgcgga acggcacacg gtaaagccac agccgccgcc cgtcggcgca ctcgatgacc
      901 ttggtcggcg cgaagccggg cgcgtcgaac tcgaggctga cgagccaggc gccgggccgc
      961 agctcgcgcg tcgccttcgc ggccgcgcgg tccatgctct cgggtcgctg gaagacatag
     1021 acgaggtcgt agccggacca gtccgcggcc cagatgtcac cgcgccgcac cgtcgcgaag
     1081 cggcagcccc aggcgcatgc gatgcgcagc ggccagctcc attcgaggcc gtgcgtcgcg
     1141 gccgccgggt actcgcgatg cagctcgcgc aggccggcgc cgaggccgca gccggcatcg
     1201 agcacgcgtg cgttcggtgc cagcggcgcc agccgcgcca gcccgtcgag cgcgccggcg
     1261 ggggtcggga acagcggcgc gtcgtgccag gcgttcagcg gatagaccag cgcgagcagc
     1321 gccagcggca gcagccaacc ccaggccggc atcgcgccgg ccaggcccga ggcgagcagc
     1381 gacagcggaa agccggccgc gacgaagacg cggcgccacg gcgtcgtgcc gagcgcgctc
     1441 aacgccgcgc cgagcgcgat cggcacggcg atcgccagcg cgagtggcgc gcccagagag
     1501 cgcagcagcg cgaacactgc ccagcccagc gcccacgcca gcagcgccgg cagcggccag
     1561 gtcaggcgca acgcgggcac gtccgagtca tcagaatctg aacgcgaggc gcaggccgcc
     1621 ccagtgcttg cccttcacgg tgatcggcgc ggccgcttcc ttcatcacga cgaagttgcc
     1681 gccgcccatg tcgcggcggt aggtctggag caggaacggc cgccggttgc gcgtcgaggc
     1741 gagaccggtg cggtcgttga agatgcggcg gtagcggctg ttggcggtgt cccagaccag
     1801 gtcgccgcgc tgcggcacgt tgtgcttctt gttgtgcgtg ggcacatagc cgttgcggtc
     1861 gccggccatg cagaacacga ccttcggact cacctcgagc atgcgctcga gcacctgcgg
     1921 aaacagcttg tcggtgaccg gcgtgaagcg agtcaggtgc tgcggcggat tcgtcccgac
     1981 gatgggtcgg tagacctcgt cgaacaggac atcgagcgtg gtcgcgccgc cggccagcgc
     2041 cgcgtcgagg atcgcgccga gctggttcgc gccttccatc acggcctgga tgtagggcgt
     2101 gtcctcggtc tcgatgccgc actcggcgac gatctcgatc aggtgctccg acacgtcgag
     2161 aaaggtctcg ctgcgcttca tcgcggcgcc gagcacgccg ctggtgcggt ccatctcggt
     2221 gacgatgccg ctcatctgcg cgtcgaggcc gccgcagatc gagcggctct gctcggcggc
     2281 cgtgatgatg ctcgcgaccg ccgcctgcac gtcggccagc gccgcgtgga actcgccctg
     2341 ctgcgccgcg ttcgcgtcga aacggatctc gcgcgcgagc gtgtcgatgc gccggtcgag
     2401 cacgccgacg gtgcgcatga tctccttcga cgagaactcg accttgccgg ccaggtcctt
     2461 gaccgcgtcg gcgacgacgc cgaagccgcg cccggcgtcg ccggcacgct tggcctcgac
     2521 cgacgcgttg aacgcgacca gccgggtc
//
LOCUS       DASRDG010000655         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_673368, whole genome shotgun sequence.
ACCESSION   DASRDG010000655 DASRDG010000000
VERSION     DASRDG010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673368"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VFA35_10980"
     CDS             <1..1488
                     /locus_tag="VFA35_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZT56743.1"
                     /translation="GSPVPSAFQPLSGVAVIATNTWAAMNGRKALKLDWDAGPNAVYA
                     SDSYRAAMEAAATRPGKLIRNHGDVDGAMKTAAKTVTAQYYMPHLTQAPMEPPAATAR
                     IKDGRCEVWGPFQSPQAVRSDVAKRLELPADKVTVNVTLLGGGFGRKSKGDFATEAAL
                     LARETGGKPVKVVWTREDDLHHSYYHTVSVERLEAGLDAGGKPVAWLHRSVGPTLMAL
                     FMPDPKTESPIELGMGLANVPFAVPNMRVENPEAAAYTRIGWYRAVSNIPHAFAVQSF
                     VCEMANAAGRDPKDYLLELLGPPRRIDPRDINDTWNHGEDPTAYPIDIGRLRRVATTV
                     AKEANWGKPLAKGRGQGIAAHYSFTTYVAAVVEVSVDAAGKLTIPQVDIAIDCGAIVN
                     PERVRAQMEGACMMGLGNALTGEISFKDGRAQQDNFHQFEVLRMNAAPKKIVVHLIPG
                     DYDAPLGGAGEPGVPPIAPALANAIFAATGKRIRNLPIRDQLRSA"
     gene            complement(1612..>2133)
                     /locus_tag="VFA35_10985"
     CDS             complement(1612..>2133)
                     /locus_tag="VFA35_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56744.1"
                     /translation="ALRWPDRVRSLTLYEPVRFALLTHAPALSESADWITGVGRRIGM
                     RVLSGARHAAAAMFVDYWSGEDSWPLLSASQRNALAERMPKVQAEFEALFADRVPPAA
                     YRELRMPVTLLGGSRSPLPVRQVLDLLEPQFPNCRRVHFDGLGHMGPMQDPQRVLAEV
                     ETSVESPSWPDAA"
BASE COUNT          357 a          758 c          744 g          274 t
ORIGIN      
        1 ggctcaccgg tcccgagcgc tttccagccg ctgtccggcg tggcggtgat cgcgaccaac
       61 acctgggccg caatgaacgg ccgcaaggcg ctcaagctcg actgggacgc cggcccgaac
      121 gcggtctatg catccgacag ctaccgcgcc gccatggaag cggccgcgac acgccccggc
      181 aagctgatcc gcaaccatgg cgatgtcgac ggggcgatga agacggccgc caagaccgtg
      241 acggcgcagt actacatgcc gcacctgacg caggcgccga tggagccgcc ggccgcgacc
      301 gcgcggatca aggacggccg ctgcgaggtc tggggcccgt tccagagccc gcaggcggtg
      361 cgcagcgatg tggccaagcg gctcgaactg ccggccgaca aggtgacggt caacgtcacc
      421 ctgctgggcg gcggcttcgg ccgcaagagc aagggcgact tcgcgaccga ggcagcgctg
      481 ctcgcgcgcg agaccggcgg caagccggtc aaggtggtgt ggacgcgcga agacgacctg
      541 caccattcct actaccacac ggtctcggtc gagcgcctgg aagccgggct cgacgcgggc
      601 ggcaagcccg ttgcctggct gcaccgcagc gtcgggccga cgctgatggc gctgttcatg
      661 cccgacccga agaccgagtc gccgatcgag ctcggcatgg ggctggccaa cgtgccgttc
      721 gcggtgccca acatgcgcgt cgagaatccg gaggccgcgg cctacacgcg cattggctgg
      781 taccgcgcgg tgtcgaatat tccgcacgcg ttcgcggtcc agtccttcgt ctgcgaaatg
      841 gccaacgcgg ccgggcgcga cccgaaggac tacctgctcg agctgctcgg gccgccgcgc
      901 cggatcgacc cgcgcgacat caacgacacc tggaaccatg gcgaggaccc gacggcctac
      961 ccgatcgaca tcgggcggct ccgccgcgtg gccacaaccg tggccaagga agcgaactgg
     1021 ggcaagccgc tcgccaaggg ccggggccag ggcatcgccg cgcactacag cttcaccacc
     1081 tatgtggcgg cggtggtgga ggtgtcggtg gacgcggccg gcaagctgac gatcccgcag
     1141 gtcgacatcg cgatcgactg cggcgccatc gtcaaccccg agcgcgtgcg cgcgcagatg
     1201 gagggcgcct gcatgatggg cctgggcaac gccttgaccg gcgagatcag cttcaaggac
     1261 ggccgcgcgc agcaggacaa cttccaccag ttcgaagtgc tgcgcatgaa cgccgcgccg
     1321 aagaagatcg tcgtccacct gattccgggc gactacgacg cgccgctcgg cggggcgggc
     1381 gagccgggcg tgccgccgat cgcgccggcg ctggcgaacg cgatcttcgc cgccaccggc
     1441 aagcgcatcc gcaacctgcc gatcagggat cagctgcgct cggcctgagg cagatcgacg
     1501 cgaccgcggc gcgcctcgcc tcaagcgcgc cgcgcggcgc cggcgatcgc atgcgccggc
     1561 gcgcaagctg gcatcggggt ctgaacggct gattcatggc cagcaccttc gctacgcggc
     1621 gtcaggccac gacggcgact cgacgcttgt ctcgacttcg gccagcaccc gctgcgggtc
     1681 ctgcatcgga cccatgtggc ccaggccgtc gaagtgcacg cggcggcaat tcgggaactg
     1741 cggttcaagc agatccagaa cctgtcgcac cggcaagggc gagcggctgc cgccgagcag
     1801 cgtcaccggc atacgcagct cgcggtaggc agccggtggc acgcggtcgg cgaacaacgc
     1861 ctcgaattcg gcctgcacct tgggcatgcg ttctgccagt gcgttccgct ggctggcgct
     1921 gagcagcggc cagctgtcct cgccgctcca gtagtcgacg aacatcgcgg ccgccgcatg
     1981 ccgcgcgccc gacagcaccc gcatgccgat gcgccgcccg acaccggtga tccagtcggc
     2041 cgactcgctc agcgccggcg cgtgggtcag cagcgcgaag cgcacgggct cgtacagggt
     2101 caggcttctg acgcgatcgg gccagcgcaa cgc
//
LOCUS       DASRDG010000656         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_556913, whole genome shotgun sequence.
ACCESSION   DASRDG010000656 DASRDG010000000
VERSION     DASRDG010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556913"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="VFA35_10990"
     CDS             <1..1444
                     /locus_tag="VFA35_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZT56745.1"
                     /translation="AGNVGGAAMTTLTALTLRGHAWTLAHVGDSRAWMIRGAGDACTQ
                     LSTDHALDHPDQRSGLTRAVGLDDRVRIDFLQGEVQVGDVFVLTTDGVHGVLKPARLA
                     ALARAGEAAGAAEAIVRAALEAGSADNASAAVLRVRGLAQGRLEDSLLQGRQLPVPGR
                     LKVGDRVDGCTITALVADNGVHRLYQARLDATQQLVALKTLHEARASDREERAMLAHE
                     AWLASRVAPGSGEQGGGAFVRTHELHEPSAFYTVYDWHAGRTLEQLLAGDHRFAVTDI
                     ATAGLALARALGRLHRHGVVHRDIKPGNLHLGDDGVWRVLDLGVAVSGREPKSLRTLH
                     AGTPSYMNPEQWNTDEHVPADARSDLYALGVTLYQWLTGKLPYGEVEPYQSGRYRRDP
                     KPPSRLRPEVPIWLDHVVLKAIALDPRLRFETAEEFALALARGASRPLPAPGTTPLAV
                     RDPRVLWQMGLGVSVLFNLLLIYWLLFLPR"
     gene            complement(1490..>2248)
                     /locus_tag="VFA35_10995"
     CDS             complement(1490..>2248)
                     /locus_tag="VFA35_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT56746.1"
                     /translation="LAAATMVLPMLVMWFCAKEPPDREHQTLREHTACLFEKDGWAFS
                     LIYIITFGGFIGLASFLPTYFYDQFKVTKVEAGQLTMLATLMGSAVRVVGGYISDRVG
                     GISTLSGVLILVAVSLVLCGLAGTSLAVTTLLFMLCFAALGAGNGALFQLVPLRWPLT
                     TAVAGSMIGEIGALGGGFLPNAMGQSKQLAGTYLWGFVSFAVLALVMLVMLRWVQIRW
                     TRTWAEKGGRARATSLPSEAGFAPTTFGAQSARP"
BASE COUNT          346 a          794 c          814 g          294 t
ORIGIN      
        1 cgcgggcaat gtcggtggcg cggccatgac gacgctgacc gcgctgacac tgcgcggcca
       61 tgcgtggacg ctggcgcacg tcggcgacag ccgtgcctgg atgatccgcg gcgcgggcga
      121 tgcctgcacc cagctcagca ccgaccacgc cctcgatcac cccgaccagc gcagcggcct
      181 gacccgggcg gtggggctcg acgaccgcgt gcgcatcgac ttcctgcaag gcgaagtgca
      241 ggtcggcgat gtgttcgtgc tgacgaccga tggcgtgcac ggcgtgctga agcccgcgcg
      301 cctggccgcg ctggcgcgcg cgggcgaagc ggcgggggcg gccgaggcga tcgtgcgcgc
      361 cgcgctggag gcgggcagcg ccgacaacgc cagtgccgcg gtgctgcgcg tgcgcggcct
      421 ggcgcagggg cgcctcgagg acagcctgct gcagggtcgg cagctgccgg tgccgggtcg
      481 cctcaaggtc ggcgaccgcg tcgacggctg cacgatcacc gcgctggtcg cggacaacgg
      541 cgtgcaccgg ctctaccagg cgcgcctcga cgcgacgcag caacttgtcg cgctgaagac
      601 gctgcacgag gcgcgcgcca gcgaccgcga ggagcgtgcg atgctcgcgc acgaggcctg
      661 gctcgcgagt cgcgtcgcgc cgggttcggg cgagcagggc ggcggcgcgt tcgtgcgcac
      721 ccacgagctg cacgagccga gcgcgttcta cacggtgtac gactggcacg ccgggcggac
      781 cctcgagcaa ttgctggcgg gcgaccaccg gttcgccgtg accgacatcg cgaccgccgg
      841 gctcgcgctg gcgcgcgcgc tcggccgctt gcatcgtcac ggcgtcgtcc accgcgacat
      901 caaaccgggc aacctgcacc tcggcgacga cggcgtctgg cgcgtcctcg acctcggtgt
      961 ggcggtttct ggacgcgagc ccaagtcgct gcgcacgctg cacgccggca cgccgagcta
     1021 catgaacccc gagcagtgga acaccgacga gcacgtgccg gccgacgcgc gcagtgacct
     1081 gtacgccctc ggcgtgacgc tgtaccagtg gttgaccggc aagctgccct acggcgaagt
     1141 cgagccgtac cagagcgggc gctaccggcg cgacccgaag ccaccgtcgc gcttgcgccc
     1201 cgaggtgccg atctggctcg accacgtcgt gctgaaggcg attgcgctcg acccgcgctt
     1261 gcgcttcgag accgccgagg aattcgcgct cgcactcgcc cgcggcgcgt cgcgcccgct
     1321 gcccgcgccc gggaccacgc cgctggcggt gcgcgacccg cgcgtgctct ggcagatggg
     1381 gctgggcgtg tccgtgctgt tcaacctgct gctgatctac tggttgctgt tcctgccgcg
     1441 ctgagcggca gggcaaagga gagcggtgca cggcaatggc ctcgtgtggc tacggccgtg
     1501 cgctttgcgc gccgaaggtc gtcggcgcaa agccggcctc ggacggaagg cttgtcgcgc
     1561 gggcgcggcc acccttctcg gcccaggtgc gcgtccagcg gatctggacc cagcggagca
     1621 tgaccagcat gacgagtgcg agcacggcga agctcacgaa gccccacagg taggtcccgg
     1681 ccaattgctt cgactggccc atggcgttcg gcagaaagcc gccgccgagc gcgccgatct
     1741 cgccgatcat cgagccggcg accgccgtcg tcagcggcca gcgcagcggc acgagctgga
     1801 acagcgcgcc gttgcccgcg ccgagggcag cgaagcaaag catgaacagc agcgtcgtca
     1861 ccgcgagcga ggtgccggcg aggccgcaca gcacgagcga gacggcgacc aggatcagca
     1921 cgccggagag tgtcgagatc ccgccgacgc gatcggagat gtagccgccg accacgcgca
     1981 ccgccgagcc catcagcgtg gccagcatcg tgagctgccc ggcctcgacc ttggtgacct
     2041 tgaactggtc gtagaagtag gtcggcagaa agctcgccag cccgatgaag ccgccgaagg
     2101 tgatgatgta gatcaggctg aaggcccagc cgtccttctc gaacaggcag gcggtgtgct
     2161 cgcgcagcgt ctggtgctcg cgatccggcg gctccttggc gcagaaccac atcaccagca
     2221 tcggcagcac catcgtcgcg gcggccag
//
LOCUS       DASRDG010000657         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_216325, whole genome shotgun sequence.
ACCESSION   DASRDG010000657 DASRDG010000000
VERSION     DASRDG010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216325"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFA35_11000"
     CDS             complement(<1..854)
                     /locus_tag="VFA35_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT56747.1"
                     /translation="MSKKFARSLKRSFVYGARDIGRLLFGRLLPRATPDALRPSEFWA
                     VRDVSFALHRGRSLGIVGQNGSGKTTLLRMVAGVLRPTLGSVRADGAIAPMLALGAGF
                     KPVLSGRENVFLNLALLGVGERAARARFDAILDFAELHDAIDAPLGTYSSGMQARLGF
                     ACAVHTEPSILIVDEVLAVGDARFRMKCRNRINELRRAGTALLLVSHSPILVEALCDE
                     ALLLRGGRMAQLGPPREVLKAYEDELLRHAVAGDAGRSPARVERPLPEGAQLAVRGVD
                     LHTPGGGSG"
     gene            complement(893..1711)
                     /locus_tag="VFA35_11005"
     CDS             complement(893..1711)
                     /locus_tag="VFA35_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56748.1"
                     /translation="MNARPHGWRARAAGAAAAVALSWRIFARTIGAKYRRSFLGYFWM
                     VAPALLITGTVVLANRAGVVTPGATELPYWLWVFLGTLVWQVFADAVDVPHEAFESAR
                     SYLTRVAFPREAIVLAQLYETLIGAGVRVVLALLVLAAAHGVGVAGAGIVALCFLGAV
                     LLGLGVGAVLMPFTQLFADLQSTKKLLLAYGLFLTPAIARPQGDGLFATVVRCNPVSP
                     LIDAARQGAAGVALVEPLRFAAVIAAGALVAALGLLLVRAVAPIVVERMLLGGR"
     gene            complement(1708..2604)
                     /locus_tag="VFA35_11010"
     CDS             complement(1708..2604)
                     /locus_tag="VFA35_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56749.1"
                     /translation="MQPQRHRAPEFAAFHAARPPGADGPHPNANLDADGYLRMLDLLF
                     RSARLFHRDAVCTLLTDRETRVDGVRGPWRRVEAPVDHGALMLSRAQAQLQWVEQRDF
                     TRPLALLDSDILLAGPLQALFDGSFDVALTWRANPEMPINGGLLLLNDRRPEVARRFF
                     RRFVAVYRERYAGANAGWYGDQLALRDCVGIAAAQMAQQPAVDVDGCRVRLLPCSSHN
                     FSPGNSFAAIANGLPGVQVLHFKGQRKRLMRPYWDAFLRPRESRLPWAALLGRRARRR
                     LQELARAEAAAGAGARAEDDAA"
     gene            complement(2677..>3162)
                     /locus_tag="VFA35_11015"
     CDS             complement(2677..>3162)
                     /locus_tag="VFA35_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56750.1"
                     /translation="QTEHCDAVLGALANSGAPLARPEAVRRLALDDSAPLRTRGRALR
                     CLESTGAVDDAAAFEQMRLRHPALLYAAARLLLTARRPAGVEMLFEVLRDGDEPPVEH
                     ADEGDARSVLEAQLGARQILAELADTSVNAEVAEWQQWWGRRPELTLTRLPAPTVTFG
                     R"
BASE COUNT          447 a         1200 c         1172 g          343 t
ORIGIN      
        1 ccggacccgc cgcccggcgt gtgcaggtcg acgccgcgca cggcgagctg cgcgccttcc
       61 gggagcggcc tctcgacgcg cgccggcgac cgcccggcgt cgccggccac cgcgtgccgc
      121 agcagttcgt cctcgtacgc cttcagcacc tcgcgcggcg gcccgagctg cgccatgcgg
      181 ccgccgcgca gcagcagcgc ctcgtcgcac agcgcctcga cgaggatcgg actgtgcgac
      241 accaacagca gcgccgtgcc ggcgcggcgc agctcgttga tgcggttgcg gcacttcatg
      301 cggaagcgcg cgtcgccgac cgcgagcacc tcgtcgacga tcaggatcga cggctcggtg
      361 tgcaccgcgc acgcgaagcc gaggcgcgcc tgcatgcccg agctgtaggt gccgagcggc
      421 gcgtcgatcg cgtcgtgcag ttcggcgaag tcgaggatcg cgtcgaagcg cgcccgcgcc
      481 gcgcgctcgc cgacgccgag cagcgcgagg ttcaggaaca cgttctcgcg gcccgacagc
      541 accggcttga agccggcgcc gagcgccagc atcggcgcga tcgcgccgtc ggcgcggacg
      601 ctgccgagcg tcggccgcag cacgccggcg accatccgca gcagcgtcgt cttgccgctg
      661 ccgttctggc cgacgatgcc cagcgaacgg ccgcggtgca gcgcgaacga gacgtcgcgc
      721 acggcccaga actcgctcgg ccgcagcgcg tccggcgtgg cgcgcggcag caaccggccg
      781 aacagcagcc ggccgatgtc gcgggcgccg tagacgaacg agcgcttgag cgagcgcgcg
      841 aacttcttgc tcacggagcg cagctcgacg cagacctcgc cgtcggccac gttcaacgcc
      901 cccccagcag catgcgctcg acgacgatcg gcgccaccgc gcgcacgagc agcagcccca
      961 gcgccgcgac cagcgcgccg gcggcgatca ccgccgcgaa ccgcagcggc tccaccagcg
     1021 cgacaccggc cgccccctgg cgcgcggcgt cgatgagcgg cgacacggga ttgcagcgca
     1081 cgaccgtcgc gaacaggccg tcgccctgcg gccgggcgat cgccggcgtg aggaacagcc
     1141 cgtaggcgag cagcagcttc ttggtgctct ggaggtccgc gaacagctgc gtgaacggca
     1201 tcagcacggc gccgacgccg agccccagca gcacggcgcc gaggaaacac agcgcgacga
     1261 tgccggcgcc ggcgacgccg accccgtgcg ccgcggcgag cacgagcagc gcgaggacga
     1321 cgcgcacgcc ggcgccgatc agcgtctcgt agagctgcgc gagcacgatc gcctcgcgcg
     1381 ggaaggcgac gcgggtcagg tagctgcgcg cgctctcgaa cgcctcgtgc ggcacgtcga
     1441 cggcgtcggc gaacacctgc cacaccagcg tgccgaggaa cacccacagc cagtacggca
     1501 gctcggtcgc gcccggcgtc acgaccccgg cgcggttcgc gagcacgacg gtgcccgtga
     1561 tcagcagcgc cggcgcgacc atccagaagt agccgaggaa cgacctgcgg tacttggcgc
     1621 cgatcgtgcg cgcgaagatg cgccacgaca gcgcgactgc cgcggcggcg ccggcggcgc
     1681 gcgcgcgcca accgtgcggg cgtgcgttca tgcggcgtcg tcctcggcgc gcgcgccggc
     1741 ccctgccgcg gcctcggccc gcgcgagctc ctgcaggcgg cgccgcgcgc ggcggccgag
     1801 cagcgcggcc cacggcaggc gcgactcgcg cggccgcagg aacgcgtccc agtacgggcg
     1861 catcagccgc ttgcgctggc ccttgaagtg cagcacctgc acgccgggca ggccgttcgc
     1921 gatcgccgcg aacgaattcc cgggcgagaa gttgtgcgag ctgcacggca gcaggcgcac
     1981 gcggcagccg tcgacgtcga cggccggctg ctgcgccatc tgcgccgccg cgatgccgac
     2041 gcagtcgcgc agcgcgagct ggtcgccgta ccagcccgcg ttcgcgcccg cgtagcgttc
     2101 gcggtagacg gcgacgaaac gccggaagaa gcggcgcgcg acctcgggcc ggcggtcgtt
     2161 gagcagcagc aggccgccgt tgatcggcat ctcggggttc gcgcgccagg tcagcgcgac
     2221 gtcgaagctg ccgtcgaaca gcgcctgcaa cggcccggcg agcaggatgt cggagtcgag
     2281 cagcgcgagc ggccgcgtga agtcgcgctg ctcgacccac tgcagctgtg cctgcgcgcg
     2341 cgacagcatc aacgcgccgt ggtcgaccgg cgcctcgacg cggcgccacg gcccgcgcac
     2401 gccgtccacg cgcgtttcgc ggtcggtgag cagcgtgcag acggcgtcgc ggtggaacag
     2461 ccgcgccgag cggaacagca ggtccagcat ccgcaggtag ccgtcggcgt ccaggttcgc
     2521 gttcgggtgc gggccgtccg cgcccggcgg ccgcgcggcg tggaaggccg cgaattcggg
     2581 cgcccggtgc cgctgcggct gcatacctgc aacgtagcgg cccggcgcgg cgcggagaag
     2641 ccgccgtccg gcgtccgctg tcgtcgcccg gctctgctaa cggccgaacg tcaccgtcgg
     2701 cgcaggcagc cgcgtgaggg tcagctccgg ccgccgcccc caccactgct gccactccgc
     2761 gacctcggcg ttgacgctcg tgtcggcgag ctcggcgagg atctggcgtg cgccgagctg
     2821 cgcttcgagg acggaccgcg cgtcgccttc gtcggcgtgt tcgacggggg gctcgtcgcc
     2881 gtcgcgcagc acctcgaaca gcatctcgac gccggccggg cggcgagcgg tgagcagcag
     2941 ccgcgccgcc gcgtagagca gcgccggatg gcgcagccgc atctgctcga acgccgcggc
     3001 gtcgtcgacg gcgccggtcg actcgagaca gcgcagcgcg cggccgcgcg tgcgcagcgg
     3061 cgccgaatcg tcgagcgcga gccggcgcac ggcttcggga cgcgcgagcg gtgcgccgct
     3121 gttcgcgagc gcgccgagca ccgcatcgca gtgctcggtc tg
//
LOCUS       DASRDG010000658         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_333752, whole genome shotgun sequence.
ACCESSION   DASRDG010000658 DASRDG010000000
VERSION     DASRDG010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333752"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="VFA35_11020"
     CDS             complement(<1..1477)
                     /locus_tag="VFA35_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZT56751.1"
                     /translation="MNLANLKIRTRLTLGFAALAALMALLGAFSIHYLLSIQHEFDEV
                     MNDRYPKLQLAGDIKAVNNDVAQALRNLFVVSDPDDIRAQYEVIAGSAKRASVAIDRL
                     TQMITSDAGKEALARLNTARAEYWVTRDKLVELLKARRAEEAKTVLLLELRPKQTAYM
                     GRVDDLIKLQDKLMVASGDEVSRTVATAKTMIGVLLAIAGIFALATAVWIIRSVTQPI
                     AEAMRIARGVADGNLSMVFEANGTSETGLLLGALHEMKTRLAHIVGDVRGNAEGVASA
                     SAQISQGNNDLSARTEQQASALQQTAASMEQLGATVRQNADNAKQADQLARGASTVAA
                     QGGEVVAQVVETMKGINDSSKKVADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVASEVRSLAQRSADAAKEIKTLIAASVERVEQGTSQVDRAGETMREVVASIRRVTD
                     IVAEISAASTEQSSGVAQIGEAVTQMDQATQQNAALVEESAA"
     gene            complement(1570..2373)
                     /locus_tag="VFA35_11025"
     CDS             complement(1570..2373)
                     /locus_tag="VFA35_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZT56752.1"
                     /translation="MNSLFSMLGIESWKPVLTALLLPPVPWLLLMLIGARLILPRRGL
                     GWFVIVLSVTGLWLSACTGSAQLLSQFVLRPPPALGSDRIQALKATKTPTAIIVLGGG
                     LEPFAPEYGVGNLYYASLERLRYGVWLSRETGLPLGFSGGVGFAQPDAKAEAQIAAGI
                     AASEFGRPLRFVEDNSRDTRENAARTVALLRPLGIREIVLVTHGWHMPRALKAFETAA
                     AGSDIRIEAAPMGLARRTETPALQWLPSSKGTTDVRNILRELLGRLAGA"
     gene            2453..>3855
                     /locus_tag="VFA35_11030"
     CDS             2453..>3855
                     /locus_tag="VFA35_11030"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HZT56753.1"
                     /translation="MKQQEIAVVWLRRLAWGVGAVLLLWAIGWLAIAPLLKWQLPLRA
                     SAALGRTVTLGDVSFHPWNLDLTLNDLAVAGLPAAKQPLLQIGQLHANLSAASLFRLA
                     PVIEALEVDALRLNVARTAEGHYDVDDLIARFTPKADAKPAGEPAHFALYNVLVRDAQ
                     LRFDDQPVRRVHAIDALHLALPFISNLPAQVEVKVEPRLAFKLNGTPFDSGAQATPFA
                     QTRSGDLKLAFAELDLAPYLGYLPASLPVKLTKGSVSADLNLNFALPPSGAPSVAVKG
                     QLGAKDLALADAAGAPLLGWQALRLGLRDVQPFARKLGFDTLHIDGLRLQATRDAAGR
                     INLLQIASPPGAAKATAAPAGRAAAAWQLSLDALELADARVLWTDAAVKPAAALQLDG
                     LSVGAKQVRWPLTKPIPVSVKGALHTQTANTPALAEFTAEGPVTDRAAKLAVKLTGVS
                     LAGFAPYLAQTLVPSVDG"
BASE COUNT          513 a         1366 c         1374 g          602 t
ORIGIN      
        1 cggcggcgct ttcttcgacc agcgctgcgt tctgctgcgt cgcctggtcc atctgcgtga
       61 ccgcctcgcc gatctgcgcg acgccgctgc tctgttcggt gctcgcggcg ctgatctcgg
      121 ccacgatgtc ggtgacgcgc cggatcgacg cgaccacctc gcgcatggtc tcgccggcgc
      181 ggtcgacctg cgacgtgcct tgctcgaccc gctcgacgct cgcggcgatc agcgtcttga
      241 tctccttcgc cgcgtcggcg ctgcgctgcg cgaggctgcg cacctcgctg gcgacgaccg
      301 cgaagccacg gccctgctcg ccggcgcgcg ccgcttcgac cgcggcgttc agcgccagga
      361 tgttggtctg gaatgcgatg ccgtcgatca cgccgatgat gtcggcgacc ttcttcgaac
      421 tgtcgttgat gcccttcatc gtctcgacga cctgggcgac gacctcgccg ccctgggccg
      481 cgacggtcga ggcgccgcgc gccaattgat cggcctgctt cgcgttgtcg gcgttctggc
      541 gcacggtggc gccgagctgt tccatcgacg ccgcggtctg ctgcagcgcg ctggcctgct
      601 gctcggtgcg cgccgacagg tcgttgttgc cttgcgagat ctgcgccgag gcgctggcca
      661 cgccctcggc gttgccgcgc acgtcgccga cgatgtgcgc caggcgcgtc ttcatctcgt
      721 gcagcgcgcc gagcagcagg ccggtctcgc tcgtgccgtt ggcctcgaac accattgaca
      781 ggttgccgtc ggcgacgccg cgggcgatcc gcatcgcctc ggcgatcggt tgcgtgaccg
      841 aacggatgat ccagaccgcg gtcgccagcg cgaatatgcc ggcgatcgcg agcagcacgc
      901 cgatcatcgt cttggcggtc gcgacggtgc ggctcacctc gtcgcccgac gcgaccatca
      961 gcttgtcctg cagcttgatc aggtcgtcga cccggcccat gtaggcggtc tgcttcgggc
     1021 gcagctcgag cagcagcacg gtcttcgctt cttcggcgcg ccgggccttg agcagctcga
     1081 ccagcttgtc gcgcgtcacc cagtactcgg cgcgcgcggt gttgagccgg gccagcgcct
     1141 ccttgccggc gtcgctggtg atcatctggg tcagcctgtc gatggcgacg ctggcgcgtt
     1201 tcgcgctgcc ggcgatgact tcgtactggg cccggatgtc gtccgggtcg gagacgacga
     1261 acaggttgcg cagcgcctgc gccacgtcgt tgttgacggc cttgatgtcg ccggcgagtt
     1321 gcagcttcgg gtagcggtcg ttcatgacct cgtcgaactc gtgctggatc gacagcaggt
     1381 agtggatcga gaacgcgccg agcagcgcca tcagcgcggc gagcgcggcg aagccgaggg
     1441 tcaggcgggt gcggattttc aggttggcga ggttcatcgt ggggcgcgag ggcaggttgg
     1501 caggaaggga agtacctgcc tgtttcggcc gaaaccgcgg cgacttgagg cgtgcgccgc
     1561 ccccgatcct caggcgccag ccaggcgccc gagcagctcg cgcaggatgt tgcgcacgtc
     1621 ggtcgtgccc ttggacgacg gcagccactg cagcgccggc gtctcggtgc ggcgcgccag
     1681 gcccatcggc gcggcctcga tgcggatgtc gctgccggcg gcggcggtct cgaaggcctt
     1741 cagcgcgcgc ggcatgtgcc agccgtgggt cacgagcacg atctcgcgga tgcccagcgg
     1801 gcgcagcagc gcgaccgtgc gcgcggcgtt ctcgcgcgtg tcgcgcgagt tgtcttcgac
     1861 gaacctcagc gggcggccga actcgctcgc ggcgatcccg gcggcgatct gcgcctcggc
     1921 cttcgcgtcg ggctgcgcaa agccgacgcc gccgctgaag cccagcggca gcccggtctc
     1981 gcggctcagc cacacgccgt agcgcaggcg ttccagcgac gcgtagtaca ggttgccgac
     2041 gccgtactcc ggcgcgaacg gctcgagccc gccgccgagc acgatgatcg cggtcggcgt
     2101 cttcgtcgcc ttcagcgcct ggatgcggtc gctgccgagc gccggcggcg gcctgagcac
     2161 gaactgcgac agcaactgcg cgctgccggt gcaggcgctg agccacagcc ccgtgacgct
     2221 cagcacgatg acgaaccagc cgagcccgcg gcgcggcagg atcagccgcg cgccgatcag
     2281 catcagcagc agccagggca ccggcggcag cagcagcgcg gtcagcacgg gcttccagga
     2341 ttcgatgccg agcatcgaga agaggctgtt caagggcggc cttgggcggg gttggcgagt
     2401 ggctcgggat tgtgcagcag taggatgcgg cggcgttggg tgaaacggca ggatgaaaca
     2461 gcaggagatc gcggtggtct ggctcagaag gttggcgtgg ggcgtgggcg cggtgctgct
     2521 gctgtgggcg atcggctggc tcgcgatcgc gccgttgctg aagtggcagc tgccgctgcg
     2581 cgcgagcgcg gcgctgggcc gcacggtgac gctcggcgac gtgagcttcc acccgtggaa
     2641 cctcgacctg acgttgaacg acctcgcggt ggccggcctg cccgcggcga agcagccgct
     2701 gctgcagatc gggcagctgc acgcgaacct ctcggccgcg tcgctgttcc gcctcgcgcc
     2761 ggtgatcgag gcgctcgagg tcgacgcgct gcgcctgaac gtcgcgcgca ccgccgaagg
     2821 ccactacgac gtcgacgacc tgatcgcgcg cttcacgccg aaggccgacg ccaagcccgc
     2881 cggcgagccc gcgcacttcg cgctctacaa cgtgctggtt cgcgacgcgc aactgcgctt
     2941 cgacgaccag cccgtccggc gcgtgcatgc gatcgacgcg ctccacctcg cgctgccgtt
     3001 catctcgaac ctgccggcgc aggtcgaggt caaggtcgag ccgcggctcg cgttcaagct
     3061 caacggcacg ccgttcgaca gcggcgcaca agccacgccg ttcgcgcaga cgcgcagcgg
     3121 cgacctgaag ctcgcgtttg ccgagctcga cctcgcgccc tacctcggct acctgcccgc
     3181 gtcgctgccg gtgaagctga cgaaaggcag cgtctcggcc gacctgaacc tcaacttcgc
     3241 gctgccgccg agcggcgcgc cgagcgtcgc ggtcaagggc caactcggcg cgaaggatct
     3301 ggcgctcgcc gacgccgcag gcgcgccgtt gctcggctgg caggcactgc gcctcggcct
     3361 gcgtgacgtg cagccgttcg cgcgcaagct cgggttcgac acgctgcaca tcgacggcct
     3421 gcggctgcag gcgacgcgcg acgcggccgg ccggatcaac ctgctgcaga tcgcgtcgcc
     3481 gcccggcgct gcgaaggcca cggccgcgcc ggccggccgc gccgcagcgg cgtggcagct
     3541 cagcctcgac gcgctcgaac tcgccgacgc gcgcgtgctc tggaccgatg cggcggtcaa
     3601 gccggccgcc gcgttgcagc tcgacggcct gagtgtcggc gcgaagcaag tgcggtggcc
     3661 gctgacgaag ccgatcccgg tcagcgtgaa gggcgcgctg cacacgcaga ccgcgaacac
     3721 gcccgcactc gccgagttca ccgccgaggg cccggtcacc gaccgcgccg ccaagctcgc
     3781 ggtgaagctg accggcgtct cgcttgccgg tttcgcgccc tacctggcgc agacgctggt
     3841 gcccagcgtc gacgg
//
LOCUS       DASRDG010000659         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_103893, whole genome shotgun sequence.
ACCESSION   DASRDG010000659 DASRDG010000000
VERSION     DASRDG010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103893"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VFA35_11035"
     CDS             <1..1121
                     /locus_tag="VFA35_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT56754.1"
                     /translation="NIASVLRFRHRREVCNAELFVALLVDVAMLTAQLYLSGGATNPF
                     VFLYLLQVILGAVLLRPWSTWTMVAVTCACFAGLALLSRPLALPLDLDRGLASPYVQG
                     MLICFALNAALLAIFVKRISGNLRARDARLADLRQRAAEEEHIVRMGLLASGAAHELG
                     TPLATLSVILGDWRRMPAFMQSRELLQELGEMQAQLARCKSIVSGILRSAGEARGESS
                     ARTTIGTFLNDLVDEWRATRPAIPFTFENRFGDDLPMVSDSALKQMICNVLDNALEAS
                     PGWIRLEAARHADALSLVVTDDGPGFPPGMLARFGKPYQSTKGRPGGGLGLFLVVNVA
                     RTLGGSVSARNRPEGGASVAVTLPLATITLAESAVHGS"
     gene            1111..1659
                     /locus_tag="VFA35_11040"
     CDS             1111..1659
                     /locus_tag="VFA35_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZT56755.1"
                     /translation="MAARKKAARRLLIVEDDAAFARTLSRSFERRGYVVQTANSFAEV
                     VALLPASSPGYAMVDLKLAGEASGLACVQALHAHDPAMLIVVLTGFASIATAVEAIKL
                     GACHYLAKPSNTDDIEAAFGRAAGNVGVELTNRSTSIKTLEWERIHETLAETGFNISE
                     TARRLGMHRRTLARKLGKPRVK"
     gene            complement(1666..>2046)
                     /locus_tag="VFA35_11045"
     CDS             complement(1666..>2046)
                     /locus_tag="VFA35_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZT56756.1"
                     /translation="VRGRRVSNWWTGLETLGAGIDDSLYGFRVYPVGALVAVMRRQPW
                     MRRFDFDTEAVVRLAWRGVKPLNLAAPVKYLTAEEGGVSHFRYGRDNALLTWMHIRLM
                     LEFALRLPLLLWRRITQRPPFQRL"
BASE COUNT          302 a          735 c          714 g          295 t
ORIGIN      
        1 tcaacatcgc cagcgtgctg cgctttcggc accggcgcga ggtgtgcaac gccgagctgt
       61 tcgtcgcgct gctggtcgac gtcgcgatgc tgaccgcgca gctctacctc agcggcgggg
      121 ccaccaaccc gttcgtgttc ctgtacctgc tgcaggtgat cctgggcgcg gtgctgctgc
      181 ggccgtggtc gacctggacg atggtggccg tcacctgcgc ctgcttcgcc ggccttgcgc
      241 tgctgtcgcg cccgctcgcg ttgccgctcg acctcgaccg cggcctggcg agcccgtatg
      301 tccagggcat gctgatctgc ttcgcgctga acgcggcgct gctcgcgatc ttcgtcaagc
      361 gcatcagcgg caacctgcgc gcacgcgacg cccgcctggc cgacctgcgc cagcgcgccg
      421 ccgaagagga gcacatcgtg cgcatgggcc tgctcgcgtc gggcgcggcg cacgagctcg
      481 gcacgccgct ggcgacgctc tcggtgatcc tcggcgactg gcggcgcatg cccgcgttca
      541 tgcagagccg cgagctgctg caggagctcg gcgagatgca ggcgcagctc gcgcgctgca
      601 agagcatcgt cagcggcatc ctgcgctcgg ccggcgaggc acgcggcgaa tcgtcggcgc
      661 gcacgaccat cggcaccttc ctgaacgacc tggtcgacga gtggcgcgcc acccggccgg
      721 cgattccgtt cacgttcgag aaccgcttcg gcgacgatct gccgatggtc tccgactcgg
      781 cgctgaagca gatgatctgc aacgtgctcg acaacgcact cgaggcttcg ccgggctgga
      841 tccggctcga agcggcgcgc cacgccgatg cgctgagcct ggtcgtgacc gacgacggcc
      901 cgggcttccc gcccgggatg ctggcgcggt tcggcaagcc ctatcaatcg accaagggcc
      961 ggccgggcgg cgggctcggg ctgttcctcg tcgtcaacgt cgcacgcacg ctgggcggca
     1021 gcgtcagcgc gcgcaaccgt cccgagggcg gcgccagcgt ggccgtgacc ctgccgctcg
     1081 cgacgatcac gctcgcagaa agcgccgtgc atggcagcta ggaagaaggc cgcgcgccgg
     1141 ctgctgatcg tcgaggacga cgccgcgttc gcgcgcacgc tgagccgctc gttcgagcgg
     1201 cgcggctacg tcgtgcagac ggcgaatagc ttcgccgaag tcgtcgcgct gctgcccgcg
     1261 agctcgccgg gctacgcgat ggtcgacctg aagctcgccg gcgaggcctc gggcctggcc
     1321 tgcgtgcagg cattgcacgc gcacgacccg gccatgctga tcgtcgtgct caccggtttc
     1381 gcgagcatcg cgaccgcggt cgaggcgatc aagctcggcg cctgccacta cctcgcgaag
     1441 ccctcgaaca ccgacgacat cgaggcggcg ttcggccgcg ccgcgggcaa cgtcggggtc
     1501 gagctgacga accgctcgac ctcgatcaag acgctcgaat gggagcgcat ccacgagacg
     1561 ctggccgaga ccggtttcaa catctccgag accgcgcggc gcctgggcat gcaccgtcgc
     1621 acgctggcgc gcaagctcgg caagccgcgc gtgaagtgac ccgggtcaca agcgttggaa
     1681 cggcggccgc tgcgtgatgc ggcgccacag cagcaacggc agccgcagcg cgaattcgag
     1741 catcagccgg atgtgcatcc aggtcagcag cgcgttgtcg cgcccgtagc ggaagtgcga
     1801 gacgccgccc tcctcggccg tcaggtactt caccggcgcc gcgaggttca gcggcttgac
     1861 gccgcgccag gccaggcgca cgacggcctc ggtgtcgaag tcgaagcggc gcatccacgg
     1921 ctggcggcgc atcaccgcga ccaacgcgcc gaccgggtag acgcgaaagc cgtagagcga
     1981 gtcgtcgatg ccggcgccga gcgtctcgag cccggtccac cagttcgaga cgcgccggcc
     2041 gcgcac
//
LOCUS       DASRDG010000660         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_334319, whole genome shotgun sequence.
ACCESSION   DASRDG010000660 DASRDG010000000
VERSION     DASRDG010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334319"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFA35_11050"
     CDS             <1..735
                     /locus_tag="VFA35_11050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZT56757.1"
                     /translation="HLQASRSRLSEIVITHKAPGDVVRAIDHEARTLIGDVVMRHFPG
                     HSFAGEAGVEAAELLADGRPAWIVDPIDGATNYLRGYPQYAVTIALVEAGEPQVGVVY
                     DPSRNEFFGAIRGRGAVLNGQAIRCAKPRPAGESLAATVFPKPASPRMPIYMAELGRV
                     LRAFSGVRRSGSMALELAYLAAGRIDAFWQHEMRAWECAAGAVLLREAGALIHARDGL
                     PLLASRSLLACTPTLFEPFLSLLAEA"
     gene            886..1791
                     /locus_tag="VFA35_11055"
     CDS             886..1791
                     /locus_tag="VFA35_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZT56758.1"
                     /translation="MNKPTLAGRNFPTAQQDASKDTANALARYQGPESSYKLAFTDTP
                     FLLREEMRPVRMQLELLKPELIQDEQGIESTVVIFGSARIVSPEVAQRLVDDASSAGD
                     EAALAIAQRHLSMSHYYEEARRFGALVTEKSKQLETPLYVVTGGGPGIMEAGNRGAFD
                     VGGKSIGLNIVLQHEQAPNPYVTPELCFQFHYFGLRKMHFLMRSVALVCFPGGFGTLD
                     EMFEALTLIQTGKSRRRPVLLFGRAFWTRLINFDLLVETGMISALDLQLFRFVETAEE
                     AWGVLEAEYGFQLRESPKDLPPVDL"
     gene            1805..2722
                     /gene="rocF"
                     /locus_tag="VFA35_11060"
     CDS             1805..2722
                     /gene="rocF"
                     /locus_tag="VFA35_11060"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HZT56759.1"
                     /translation="MTSTRISLIGAPTDIGAGSRGASMGPEALRVANIGPVLVSHGLE
                     VIDRGNLAGPPNPWLPPVDGYRHLHEVVAWNQTVHGAVHAELQQGRLPILLGGDHALS
                     IGSISAVARHCRESGKKLRVLWLDAHADFNTNGLTPSGNLHGMPVACLCGNGPAALCE
                     IGGVVPAISPKWVRQIGIRSVDPGEKRLVHEAGLDVFDMRYIDEMGMRHAMELALATL
                     DANTHLHVSFDVDFLDASIAPGVATTVKGGPTYREAQLCMEMIADTGRLASLDVMELN
                     PALDVRNGTAIVAVDLIESLFGKSTLMRR"
BASE COUNT          447 a         1024 c          954 g          415 t
ORIGIN      
        1 cacctgcagg cgtcgcgatc ccggctctcc gagatcgtga tcacccacaa ggcgcccggc
       61 gacgtggtcc gcgcgatcga ccacgaggcg cgcacgctga tcggcgacgt ggtcatgcgc
      121 cacttccccg gccacagctt cgccggcgag gcgggggtgg aggccgccga gttgctcgcc
      181 gacggccgcc cggcctggat cgtcgacccg atcgacggcg ccaccaacta cctgcgcggc
      241 tacccgcagt acgcggtcac gatcgcgctg gtcgaggccg gcgagccgca ggtcggcgtg
      301 gtctacgacc cgagccgcaa cgaattcttc ggcgcgatcc gcggccgcgg cgcggtgctc
      361 aacggccagg cgatccgctg cgccaagccg cggccggcgg gcgagtcgct cgcggccacg
      421 gttttcccca agccggcgag cccgcgcatg ccgatctaca tggccgagct cggccgcgtg
      481 ctgcgcgcgt tcagcggcgt gcgccgctcg ggctcgatgg cactggaact tgcctacctc
      541 gcggccggcc gcatcgacgc gttctggcag cacgagatgc gcgcctggga gtgcgccgcc
      601 ggcgccgtac tgctgcgcga ggccggcgcg ctgatccacg cgcgcgacgg cctgcccctg
      661 ctcgccagcc gctcgctgct ggcctgcacg cccacgctgt tcgagccctt cctgagcttg
      721 ctcgcggaag cctgaaccgc gggacctggc acggctcggg ccacccgccg ggccgcccca
      781 agcaaggccc ggccaagagc cacgcagggg cccctggcgg ccccgggatg gctgatcagc
      841 gtacccgtcg atcggggtgt cgacatattc gcttaagctg cgtccatgaa caaacccacc
      901 ctcgccggcc gcaactttcc gaccgcccag caagacgcct cgaaggacac ggccaacgcc
      961 ctcgcccgct accagggccc cgaaagctcg tacaagctcg ccttcaccga caccccgttc
     1021 ctgctgcgcg aagagatgcg cccggtgcgc atgcagctcg agttgctcaa gcccgaactg
     1081 atccaggacg aacagggcat cgagtcgacc gtcgtgatct tcggcagcgc gcgcatcgtg
     1141 tcgcccgagg tggcgcagcg cctggtcgac gatgcgagct cggccggcga cgaagcggcg
     1201 ctggcgatcg cgcagcggca tctgagcatg tcgcactact acgaagaggc gcggcgcttc
     1261 ggcgcgctcg tgaccgagaa gtcgaagcag ctcgagaccc cgctctatgt cgtcaccggc
     1321 ggcggccccg gcatcatgga ggcgggcaac cgcggcgcgt tcgacgtcgg cggcaagagc
     1381 atcggcctga acatcgtgct gcagcacgag caggcgccga acccctacgt cacgccggag
     1441 ctgtgcttcc agttccacta cttcggcctg cgcaagatgc acttcctgat gcgctcggtc
     1501 gcgttggtgt gcttcccggg cggcttcggc acgctcgacg agatgttcga ggcgctgacg
     1561 ctgatccaga cgggcaagtc gcgccgccgc ccggtgctgc tgttcggtcg cgcgttctgg
     1621 accaggctga tcaacttcga cctgctcgtc gagaccggca tgatcagcgc gctcgacctg
     1681 cagctgttcc gcttcgtcga gaccgccgaa gaagcgtggg gcgtgctcga agcggagtac
     1741 ggcttccagc tgcgcgagag ccccaaggac ttgccgccgg tcgatctttg agaggccgca
     1801 gcgcatgacg agcacccgca tcagcctgat cggcgcgccg accgacatcg gcgccggcag
     1861 ccgcggcgcc agcatgggcc ccgaggcgct gcgcgtggcc aacatcggcc cggtgctggt
     1921 gagccacggc ctcgaggtga tcgatcgcgg caacctcgcc ggcccgccga acccgtggct
     1981 gccgccggtc gatggttacc ggcacttgca cgaagtcgtc gcgtggaacc agacggtgca
     2041 cggcgcggtg catgccgagc tgcagcaggg ccgcctgccg atcctgctcg gcggtgacca
     2101 tgccttgagc atcggctcga tcagcgcggt cgcgcgccat tgccgcgaga gcggcaagaa
     2161 gctgcgcgtc ctctggctcg acgcgcacgc cgacttcaac accaacggcc tcacgcccag
     2221 cggcaatctg cacggcatgc cggtcgcatg cctgtgcggc aacggccccg cggcgctgtg
     2281 cgagatcggc ggcgtcgtgc ccgcgatcag cccgaagtgg gtgcgccaga tcggcatccg
     2341 cagcgtcgac ccgggcgaga agcggctcgt gcacgaggcc ggcctcgacg tcttcgacat
     2401 gcgctacatc gacgagatgg gcatgcgcca cgcgatggag ctcgcactcg cgacgctcga
     2461 cgcgaacacc cacctgcacg tgagcttcga cgtcgacttc ctcgacgcct cgatcgcccc
     2521 cggcgtggcc accaccgtga aaggcggccc gacctaccgc gaagcgcagc tgtgcatgga
     2581 gatgatcgcc gacaccggta ggctcgcgtc gctcgacgtg atggagctga acccggcgct
     2641 cgacgtgcgc aacggcaccg cgatcgtcgc ggtcgacctg atcgagagcc tgttcggcaa
     2701 gagcacgttg atgcggcgct gagatccagt attcgaggaa ggtttcaatg caagtcaccg
     2761 ggcggcaaat cctgccggcg gtactcgtat tcatgatgtt gcaggcatac gccggtgcgc
     2821 agcctctgcc accgccggag
//
LOCUS       DASRDG010000661         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_674859, whole genome shotgun sequence.
ACCESSION   DASRDG010000661 DASRDG010000000
VERSION     DASRDG010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674859"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFA35_11065"
     CDS             <1..673
                     /locus_tag="VFA35_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56760.1"
                     /translation="VGTGRALQVYSGNGEPQLQLPPHPGVIAALAWKAKGSELAAVGN
                     GGARIHRLEPPQQTREYACPGACLTASWSPDGRFLAAGMQDGSVHYWNLAAGTQSQMR
                     GYGAKVSLTCWSANGRLLATAADEQIVVWDFGGRGPEGSEPLQLAAHTARVTALACQP
                     RGTLIAAGARDHRLTLWRPAQPGQPVDADLLADEVALLRWSNDGAQLVAADRGGNILL
                     YALRQ"
     gene            complement(688..3081)
                     /locus_tag="VFA35_11070"
     CDS             complement(688..3081)
                     /locus_tag="VFA35_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZT56761.1"
                     /translation="MTPRTLIAGRDRQQRLWLRHHLQTLWPDAEPPSLDLQQLEAHVD
                     TITRRNYDIVLLCASFDAAVGEQTEGLGWLRRLRRGRNRPPVIIVAAGGNEIAAVRSV
                     RLGAAAYLPRDLLDAQLLERTLRKVLQAGNRRQRRLASIRRRRARDAGGLEIPNYTLL
                     RRLGRSARASVWLAYSDSLQRHVAVKISQPLDGLPTDQQQFAREYAAIAALRDPAIVD
                     IHQHGIHEGREFLAMEYFPCGDLKQRMLNPMTSAEAVGYTRRIAAALAVVHGSGMMHR
                     DLKPPNIMLRPDGSVVLIDFGLAKRVNSDTQSTAIGVLRGSPYYMSPEQVQGQVLDAR
                     SDQYSLGVVLYEMLAGRKPYTGLTAMDLMQQHVSGERPALPAPLAHFEPLIARLMARD
                     REQRYPDVGAVLEELERLQPDEAANAAAGAAPVVDGAALDPGAAAGDAAGDIAAEPAG
                     ATTVAMLQLRGQRDLLAELLLLERSALAYFMPVAARIMARVRALLRQRSHEHGAFREK
                     LERLQRLYARLRRRAAALPLEALTDLLDEAMAALAAALALEAPTGDALLPVLARNDAV
                     FLALATVADCSGIPLRARRAVPGRARLAAVPAGGGGDEPASQLALALQQLADQLATEQ
                     GKLVELTTIGLEKIPENQAAACYDMLSQMLRNSIEHGIETPAQRRAAGKGTRGSLLVE
                     FQWRLGGQSELNFQDDGQGLDAERIVQVAVANGLVTDDTSLEQNRRQASALIFHSGLS
                     TAAEPAGRGFGMRILRDHIKRLRGQIQVATKRGQFTRIRIRLPTPAAAADSGKKALA"
     gene            3291..>3730
                     /locus_tag="VFA35_11075"
     CDS             3291..>3730
                     /locus_tag="VFA35_11075"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT56762.1"
                     /translation="MDGPVVIIGAGLAGLQVAESLRHEKYEGPITLLGAEPHAPYNRP
                     PLSKQWLREQRPTSTLHIRGLEAIARRHIDLRVRTTASAVDRDARIVHCADGGKFPYA
                     GLALATGARMRTLPVPGVGLAGVHGLRTIDDAERIAAALARCAAR"
BASE COUNT          516 a         1296 c         1350 g          568 t
ORIGIN      
        1 ggtcggcacg ggtcgagcgc tccaggtcta ctccggcaac ggcgaaccgc agctgcagtt
       61 gccgccgcat cccggcgtca tcgccgcgct tgcctggaag gcaaaaggaa gcgaactcgc
      121 cgccgtgggc aatggcggtg cgcgcatcca tcggctcgag ccgccgcagc agacgcgcga
      181 atacgcgtgc cccggcgcct gcctgacggc gagctggagc ccggacggac gtttcctggc
      241 tgcgggcatg caagacggtt cggtgcatta ctggaacctc gctgccggca cgcagtcgca
      301 gatgcgcggc tacggcgcca aggtgtcgct cacctgctgg agcgccaacg ggcggctgct
      361 ggcgacggcc gccgacgagc agatcgtggt ctgggacttc ggcggtcgcg ggccggaagg
      421 cagcgagccg ctgcagctgg cggcgcacac cgcgcgcgtg acggcgctcg cctgccagcc
      481 gcgcggaacg ctgattgcgg cgggtgctcg cgatcaccgg ctcacgctct ggcggccagc
      541 gcagcccggg cagcccgtcg atgccgacct gctcgccgac gaagtcgcgc tgctgcgctg
      601 gtcgaacgac ggcgcgcagc ttgtagctgc cgatcgcggc gggaatatcc ttctgtacgc
      661 gctgcgccag tagcggcgcg gcggacctca ggccagcgcc ttcttcccgg agtcggcggc
      721 cgcggccggc gtgggaagcc ggatgcgaat gcgggtgaac tgcccgcgct tggtcgccac
      781 ctggatctgg ccacgcaggc gcttgatgtg gtcgcgcagg atgcgcatgc cgaagccgcg
      841 ccccgcaggt tcggccgccg tgctgaggcc ggagtgaaag atgagcgccg aggcctggcg
      901 tcgattctgc tcgagcgagg tgtcgtcggt gacgaggccg ttggccaccg ccacctggac
      961 gatgcgttcc gcatcgagtc cctggccgtc gtcctggaaa ttcagctccg attggccgcc
     1021 gagccgccac tggaactcca ccagcagcga gccgcgcgtg cccttgccgg ccgcgcggcg
     1081 ttgcgccggc gtctcgatgc cgtgctcgat cgaattgcgc agcatctggc taagcatgtc
     1141 gtagcaggcg gccgcctggt tctcgggtat cttctcgagg ccgatggtcg tcagctccac
     1201 gagcttgcct tgctcggtcg cgagctggtc ggcaagctgt tgcagcgcca acgcgagttg
     1261 cgacgccggc tcgtcgccgc cgccgccggc cgggacagcc gcgaggcgtg cgcgcccggg
     1321 cactgcacgc cgcgcgcgca acgggatgcc gctgcaatcg gcgaccgtgg cgagtgcgag
     1381 gaacaccgcg tcgttgcgcg ccagcactgg cagcagcgca tcgcccgtcg gtgcttccag
     1441 tgcgagcgcc gcagccagcg ccgccatcgc ctcgtcgagc aggtccgtca gcgcctccaa
     1501 gggcagcgcc gcggcccgcc gccgcaggcg cgcgtacagg cgctgcaggc gctcgagctt
     1561 ctcacggaaa gccccgtgtt cgtggctgcg ctgccgcagt aatgcccgca cgcgcgccat
     1621 gatgcgcgcc gccacgggca tgaaataggc gagggcgctg cgctcgagca gcagcagttc
     1681 cgcgagcagg tcgcgctgcc cgcgaagttg cagcattgcc accgtggtgg cgcctgcggg
     1741 ctccgcggcg atgtcacctg ccgcgtcgcc ggccgccgcg cccgggtcca gcgccgcgcc
     1801 gtcgacaacg ggagctgcgc cggccgccgc attggccgcc tcgtcgggtt gcaggcgctc
     1861 gagttcctcg agtacggcgc cgacgtcggg atagcgctgt tcgcgatcgc gcgccatgag
     1921 ccgtgcaatc agcggctcga agtgcgcgag cggcgcgggc agcgccggac gctcgccaga
     1981 gacgtgctgt tgcatgaggt ccatcgccgt cagcccggta tagggcttgc gcccggcgag
     2041 catctcgtag agcacgacgc cgagcgagta ctggtcgctg cgcgcgtcga gcacctggcc
     2101 ctgcacctgc tccgggctca tgtaataggg cgaaccgcgc agcacgccga tggcggtgct
     2161 ctgggtgtcg gaattgacgc gcttggcgag tccgaaatcg atcagcacca ccgagccgtc
     2221 ggggcgcagc atgatgttgg gcggcttgag gtcacggtgc atcatgccgc tgccgtgcac
     2281 gacagcgagc gccgcggcaa tgcgacgcgt gtagccgacc gcttcggcgc tcgtcatcgg
     2341 gttcagcatg cgctgcttga ggtcgccgca cgggaaatat tccatggcga ggaattcgcg
     2401 tccctcgtgg atgccatgct ggtggatgtc cacgatcgcc ggatcccgga gcgccgcgat
     2461 tgcggcgtac tcgcgcgcga actgctgctg gtccgtcggc aggccgtcga gcggctggct
     2521 gatcttgacg gcgacgtggc gctggagcga gtcgctgtag gccagccaga cgctggcgcg
     2581 agccgagcgc ccgagccggc gcagcagcgt gtaattgggg atctcgagtc cgcccgcatc
     2641 gcgtgcgcgg cggcggcgaa tgctggccag gcggcgctgg cgccggttgc cggcctgcag
     2701 caccttgcgg agcgtgcgct ccagcagctg cgcgtccagc aggtcgcgcg gcaggtaggc
     2761 cgcggcaccg agccgcaccg agcgcacggc cgcgatctcg ttgccgccgg ccgcgacgat
     2821 tatcacgggc ggccgattac gtccgcgccg cagccgccgg agccagccca ggccctcggt
     2881 ctgctccccg accgcagcgt cgaagctcgc acacagcagc acgatgtcgt agttgcggcg
     2941 cgtgatcgtg tcgacgtgtg cctcgagctg ttgcaggtcg aggctcggcg gttcggcgtc
     3001 cggccacagc gtctgcaggt gatgtcgcag ccacaggcgc tgctgccggt cgcggcctgc
     3061 gatcagcgta cgcggcgtca cgccgcctcc cggcgaaagt ctggggcgaa tttgcggctc
     3121 gatggtctca cgcgcgctcg acggattcga tcagttccgg tgccgggact ttacgttttc
     3181 tggctgcggg ccgagccgca cgcgccgcaa tctgtgactc acttgagccg ggcgtgccgc
     3241 gaactgtgac gccagtcgcg tttcgcgccg gacacggcta atctgccgcc gtggacggac
     3301 ccgtcgtcat aattggtgcc ggtctggccg gtttgcaggt cgccgaatcg ctccgtcacg
     3361 agaagtacga gggcccgatc acgctgcttg gagccgagcc gcacgctccc tacaaccgcc
     3421 cgccgctgtc gaagcagtgg ctgcgcgagc agcgcccgac ctcaacgctg cacatccgtg
     3481 ggctcgaggc gatcgcgcgg cggcacatcg acctgcgagt gcgcacgacc gccagcgcgg
     3541 tcgatcgcga cgcgcgtatc gtgcattgcg ccgacggcgg gaagtttccc tacgctgggc
     3601 tggcgctggc gactggcgcg cgcatgcgca cgctgcccgt accgggcgtg ggactggcgg
     3661 gcgtgcacgg cttgcgcacc atcgacgacg ccgagcgcat cgccgcggcg ctcgcacgct
     3721 gcgccgcgcg
//
LOCUS       DASRDG010000662         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1708554, whole genome shotgun sequence.
ACCESSION   DASRDG010000662 DASRDG010000000
VERSION     DASRDG010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708554"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFA35_11080"
     CDS             <1..968
                     /locus_tag="VFA35_11080"
                     /inference="COORDINATES: protein motif:HMM:NF015114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56763.1"
                     /translation="LGRGDSPEHRKAKAVFAAELLGTAHHNPLLGESAAIALGLLCLP
                     PEKLTEDAQFAQALLHGWQHATDQLLRYQAALALGRIGGDSNRKTLLGIYEHSNRNIE
                     RPWVALALGVLAHQHRGTDSPVDAELGRLLLSDVQTIENGDAQSAFALALGLIGYTEA
                     APVLERMFGQVGNETLEGYLAVALALLDARGAGDAMVARMQRSVHRPFVLLQCALALG
                     RLGDARTVPTLLEMLGRADSTAALAAIATALIRIGDRRCIEPLIQAALAADRTKLARA
                     FAAAALGGIGDKDPVPWNSQIAAETNYMAAVDTLTNGATGVLDIL"
     gene            1028..>2879
                     /locus_tag="VFA35_11085"
     CDS             1028..>2879
                     /locus_tag="VFA35_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZT56764.1"
                     /translation="MRRAAVPLLACALLAAVALLLTVRSTFAPPPGPGARAPAAGAPS
                     AVRVDGADAGTAPAAAAAGDAAAADARTASAAVLDYAAERTGYRGRVVAARSLQPVPG
                     MNVTLCRGAPDLALPPGVSVFARAPIEPQIVAARARTGADGRFQLDGVLPRGLCFLRL
                     EFPDVTQAPPPWRGGQGTTVPVQRTPAPGEVVDLGDLPLKPGATLLGRVVDGQGPVAG
                     ATVRAARLPPVPFGAVPVERLRPDGALLVTAAGIRSVIELPRWVADVLAELPIPTSST
                     AADGTFALYGVDPGVNVVAATAPQHASLLRQGLAVEPDATQALGDLELPAGEEVEIAV
                     ADPSGAPIAGAEVLVAPQSTGVPVHIAERAGRTDGDGRVRVRGLPRGSALAAARRSPK
                     EEWVVGEPAPVDGEVRVTLPAAFALTLAVQGPQSKPAADVQLRLVDASANAGAVELSM
                     FGLLRGVDLDDRLRRLDDGRLRIERLRAGTWCVVVGASGCASTSFDVELRADVDRTVQ
                     LRAARALRVRTIDADGAPVAAATIYVQPRGGERAQRIVELPLAAGRTDGDGWCTVKDL
                     PTDETRITASHPRLGSVHATIAGAPPETVLQFAAPCSLRGTLTDGGRPPAP"
BASE COUNT          328 a         1104 c         1098 g          349 t
ORIGIN      
        1 tgctcgggcg cggcgactcg ccggagcacc gcaaggcgaa ggccgtgttc gcggccgagc
       61 tcctgggcac cgcgcaccac aacccgctgc tcggcgagag cgccgcgatc gcgctggggc
      121 tcctgtgcct gccgcccgag aaactgacag aggacgcgca gttcgcgcag gcgttgctgc
      181 acggctggca gcacgccacc gaccagctgc tgcgctacca ggcggcgctg gcgttgggcc
      241 gcatcggcgg cgacagcaac cgcaagacgc tgctcggcat ctacgagcac agcaaccgca
      301 acatcgaacg tccgtgggtc gcgctcgcgc tcggcgtcct cgcgcaccag caccgcggca
      361 ccgactcgcc cgtcgacgcc gagctcggcc gcctgctgct gagcgacgtg cagacgatcg
      421 agaacggcga cgcgcagtcg gcgttcgcgc tcgcgctcgg gctgatcggc tacacggagg
      481 ccgcgccggt gctcgagcgc atgttcgggc aggtcggcaa cgagacgctc gaaggctacc
      541 tcgcggtggc gctggcgctg ctcgacgccc gcggcgccgg cgacgcgatg gtcgcgcgca
      601 tgcagcgaag cgtgcaccgg ccgttcgtgc tgctgcagtg cgccctggcg ctcgggcggc
      661 tcggcgacgc gcgcaccgtg ccgacgctgc tggagatgct cggccgcgcc gacagcaccg
      721 cggcgctggc cgcgatcgcc accgcgctga tccgcatcgg cgaccggcgc tgcatcgaac
      781 cgctgataca ggccgcgctc gccgccgacc gcaccaagct cgcgcgcgcg ttcgccgccg
      841 cggcgctcgg cggcatcggc gacaaggacc cggtgccgtg gaactcgcag atcgcggccg
      901 agaccaacta catggcggcc gtcgacacgc tgaccaacgg ggccacggga gtgctcgaca
      961 tcctgtgacg gccttcttgt gactgccttc ttgtgactgg gcggaccatc ggcggttcaa
     1021 tggcgccatg cgccgtgccg ccgtcccgct gctcgcctgc gcgctgctcg ccgcggtggc
     1081 gctgctgctg acggtccgct ccacgttcgc gccgccgccc ggcccgggcg cgcgcgcacc
     1141 ggcggccggc gcgccgtccg cggtccgggt cgatggcgcc gacgccggca ccgcgcccgc
     1201 cgccgccgcc gcgggcgacg ccgctgcggc cgacgcgcgc acggcgtccg ccgcggtgct
     1261 cgactacgcg gccgaacgca cgggctaccg cgggcgcgtg gtcgccgccc gttccctgca
     1321 gcccgtgccg ggcatgaacg tgacgctctg ccgcggggcg cccgacctcg cgctgccgcc
     1381 cggcgtctcg gtgttcgcgc gcgcgccgat cgaaccgcag atcgtcgccg cgcgcgcgcg
     1441 cacgggcgcc gacggccgct tccagctcga cggcgtgctg ccgcgcggac tgtgcttcct
     1501 gcgcctcgag ttcccggacg tgacgcaggc gccgccgcca tggcgcggcg ggcagggcac
     1561 gacggttccc gtgcagcgga cgccggcgcc cggcgaagtc gtcgacctcg gcgacctgcc
     1621 gctgaaacca ggcgccacgc tgctcgggcg cgtggtcgac gggcaggggc cggtcgcagg
     1681 cgcgaccgtg cgcgccgcgc ggctgccgcc ggtgccgttc ggcgcggtgc cggtggagcg
     1741 tctgcggccg gacggcgcac tgctcgtcac tgcggcgggc atccgcagcg tgatcgagct
     1801 gccgcgctgg gtcgcggacg tgctcgccga actgccgatc ccgacgtcgt cgaccgcggc
     1861 cgacggcaca ttcgcgctct acggcgtcga tcccggcgtc aacgtcgtcg cggcgacggc
     1921 gcctcagcac gcgagcctgc tgcggcaggg gctcgcggtg gagccggatg cgacgcaggc
     1981 gctcggcgac ctggagctgc ccgcgggcga ggaggtcgag atcgccgtcg ccgacccgtc
     2041 cggcgcgccg atcgcgggcg ccgaggtcct ggtcgcgccg cagagcaccg gcgtgcccgt
     2101 gcacatcgcc gagcgcgccg gccgcacgga cggcgacggc cgcgtgcgcg tgcgcgggct
     2161 gccgcgcggc agcgcgctgg cggcggcgcg ccggtcgccg aaggaggaat gggtcgtcgg
     2221 cgagccggcg ccggtcgacg gcgaagtgcg cgtgacgctg cccgcggcgt tcgcgctaac
     2281 gctcgcggtg cagggtccgc agtcgaagcc tgcggccgac gtgcagctgc ggctcgtcga
     2341 cgcctcggcg aacgcgggcg ccgtcgagct gtcgatgttc gggctgttgc gcggcgtcga
     2401 tctcgacgac cgcctgcgcc ggctcgacga cgggcggctc cgcatcgagc ggctgcgcgc
     2461 cggcacgtgg tgcgtcgtcg tcggcgccag tggctgcgcg tcgacctcgt tcgacgtcga
     2521 gctgcgcgcc gacgtcgacc gcaccgtgca gctgcgggcc gcgcgcgcgc tgcgcgtgcg
     2581 cacgatcgac gccgacggcg cgccggtcgc ggcggcaacc atctacgtgc agccgcgcgg
     2641 cggcgagcgc gcgcagcgca tcgtcgagct gccgctcgcg gcggggcgca ccgacggcga
     2701 cggctggtgc acggtgaagg acctgccgac cgacgagacg cgcatcaccg cgagccaccc
     2761 gcgcctcggc tccgtgcacg cgacgatcgc cggtgcgccg ccggagacgg tgctgcagtt
     2821 cgcggcgccg tgctcgctgc gcggcacgct gacggacggc ggccggccgc ccgctcccg
//
LOCUS       DASRDG010000663         4726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1708878, whole genome shotgun sequence.
ACCESSION   DASRDG010000663 DASRDG010000000
VERSION     DASRDG010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708878"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFA35_11090"
     CDS             <1..312
                     /locus_tag="VFA35_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZT56765.1"
                     /translation="DCTHYVGHGITGTEGMPVTVALRPEKIRLAREAPAESEFNHARG
                     TVKDMAYFGSFTVYHLLLPSGAVLKVSTGNTERHRDHAIAVGDAAWASWAPTAQVVLT
                     S"
     gene            309..1223
                     /locus_tag="VFA35_11095"
     CDS             309..1223
                     /locus_tag="VFA35_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZT56766.1"
                     /translation="MSRTNDRTGWRVIAPPYFWLLLFFLLPFLIVLKISVSEMDVASV
                     KDVVTMQDGRLDLNLRFGNYLTLVTDDLYFSTYLSSLKYAAVTTVLCLGLGYPFAYFM
                     ARARATLQPALLMLVMLPFWTSFLLRVYAWKGLLTEHGWAHAFITSTGLDQLLLHAGL
                     IPGAGQLMNTPFSLVLGMTYTYLPFMILPLYSNLAKMDLRLLEAASDLGATAWVAFWK
                     ITVPLSKAGIIAGAMLVFIPCVGEYVIPELLGGPQTQMIGRTLWDEFFSNNDWPMASA
                     VAVAMVLLVIVPLALFNKFQAEATERHP"
     gene            1220..2062
                     /locus_tag="VFA35_11100"
     CDS             1220..2062
                     /locus_tag="VFA35_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZT56767.1"
                     /translation="MSAASLNRWFGRGWLSLGYAFLYLPIVALMIFSFNDSPVPNVWG
                     RFTLKWYAALKSDTEILSGLWLSLRIAFFTACASVVLGTLAALALVKHRRFIGRTLFS
                     GMLNAPLVMPEVIIGLSLLLMLVAMQQALGFPERGLLTIWLGHLLLGMAYAAVVVQAR
                     LKDLNPQLEEAAMDLGARPSQVFWLVTLPMISQALLSAWLLTFTLSLDDVVLSAFLSG
                     PGATTMPLVIFSRARLGLNPSVNAVATLTVLLVSIGVIGASLWIARRERARAAEIAAA
                     ARAA"
     gene            2059..2967
                     /locus_tag="VFA35_11105"
     CDS             2059..2967
                     /locus_tag="VFA35_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZT56768.1"
                     /translation="MTAAQRPHAPWLSFLCLAGSMALVGSYVGLSKVLVLVFPVFLLA
                     FLRFGIAALAMAGWVRRGATEPALNAHDRRLLFLESFFGNFLFSICMLFGVSMSSALA
                     AGVIMAGIPAAVALLSRVFLHERIAARTMLGIACAVGGIALVSLTKNAAAPAHSSLLG
                     NLLLVAAVLCEASYVVIGKKLTGNVSPKRISALINLWGLALVTPFGVWQALSFDFGAV
                     AAASWWLLAFYSIAASMVTVWLWMTGLKHVPASKAGIFTVLLPVSAAAVGVLFLGERF
                     STAQAAAFALALAGVVLATWPSRNSN"
     gene            complement(2968..3963)
                     /gene="dusA"
                     /locus_tag="VFA35_11110"
     CDS             complement(2968..3963)
                     /gene="dusA"
                     /locus_tag="VFA35_11110"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HZT56769.1"
                     /translation="MQPGEQQVFNRWRLSVAPMMDWTDRHCRFFHRLLSRHTHLYTEM
                     VTTGALLHGDVPRHLDFDAAEHPLALQLGGSEPADLAHCAKLAGQWGYDEVNLNCGCP
                     SERVQRGAFGACLMAEPQLVADCVKAMRDAVSLPVTVKHRIGIDKTESYAFVRDFVGT
                     VADAGCEVFIVHARNAWLKGISPKENRELPPLRYELVHRLKREFPALTIVLNGGIKTN
                     AQIAEQLQLLDGVMLGREPYHHPWLMADWDARFFGAPPVELDRDAIEAQMVAYMQRVV
                     AGGEPWSHVSRHMLGLRNGLPGARRWRQVWSDHRLKQADPAEVSRLARESAEAFA"
     gene            4075..4350
                     /locus_tag="VFA35_11115"
     CDS             4075..4350
                     /locus_tag="VFA35_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HZT56770.1"
                     /translation="MATLTIRNLEDALKSRLRLRAAARNRSMEDEVRHILRAALQEPA
                     APASDLGTRIRARFAGLGDVQLPVTAREPVRPPPLFDAAPAKSARRK"
     gene            4347..>4726
                     /locus_tag="VFA35_11120"
     CDS             4347..>4726
                     /locus_tag="VFA35_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZT56771.1"
                     /translation="MSTLLDTNVLSELLRAAPEPAVLRWLAAQPADGLFVTAVTQAEM
                     LLGARLLPAGKRRSALEAALRGLFDEDFAGRVLPFDSGATPAYAELVAARRAAGRPIS
                     QFDAQIAAIARRHAMGVATRDVDD"
BASE COUNT          628 a         1657 c         1621 g          820 t
ORIGIN      
        1 gactgcaccc actacgtcgg ccacggcatc accggcaccg agggcatgcc ggtcacggtg
       61 gcgctgcgcc cggagaagat ccggctcgcg cgcgaggcgc cggccgagag cgagttcaac
      121 cacgcccgcg gcaccgtgaa ggacatggcc tacttcggca gcttcaccgt ctaccacctg
      181 ctgctgccca gcggcgcggt gctgaaggtc agcaccggca acaccgagcg gcaccgcgac
      241 cacgcgatcg cggtcggcga tgccgcgtgg gcgagctggg cgccgaccgc gcaggtggtg
      301 ctgacgtcat gagccgaacg aacgatcgca ccggctggcg cgtgattgcg ccgccctact
      361 tctggctgct gctgttcttc ctgctgccgt tcctgatcgt gctgaagatc agcgtctcgg
      421 agatggacgt cgcgagcgtc aaggatgtcg tgacgatgca ggacgggcgg ctcgacctga
      481 acctgcgctt cggcaactac ctgacgctgg tgaccgacga cctgtacttc tcgacctacc
      541 tgagcagcct gaagtacgcg gccgtcacga ccgtgctgtg cttggggctg ggctacccgt
      601 tcgcctactt catggcccgc gcgcgcgcga cgctccagcc cgccttgctg atgctggtga
      661 tgctgccgtt ctggacttcg ttcctgctgc gcgtctacgc ctggaagggc ctgctgaccg
      721 agcacggctg ggcccatgcg ttcatcacct cgaccggcct ggaccagcta ctgctgcacg
      781 ccggcctgat ccccggcgcg ggccagctga tgaacacgcc gttctcgctg gtgctgggca
      841 tgacctacac ctacctgccg ttcatgatcc tgccgctgta ctcgaacctc gcgaagatgg
      901 acctgcgcct gctcgaggcc gcgagcgacc tcggcgccac cgcctgggtc gcgttctgga
      961 agatcaccgt gcccttgtcg aaggccggca tcatcgccgg cgccatgctg gtgttcatcc
     1021 cgtgcgtcgg cgagtacgtg atcccggagc tgctcggcgg cccgcagacg cagatgatcg
     1081 gccgcacgct gtgggacgag ttcttcagca acaacgactg gccgatggct tcggcggtcg
     1141 cggtggcgat ggtgctgctg gtgatcgtgc cgctggcgct gttcaacaag ttccaggccg
     1201 aagcgacgga gcggcatcca tgagcgcggc gtcgttgaac cgctggttcg gccgcggctg
     1261 gctgtcgctc ggctacgcgt tcctgtacct gccgatcgtc gcgctgatga tcttctcgtt
     1321 caacgactcg ccggtgccga acgtctgggg ccgcttcacg ctgaagtggt atgcggcgct
     1381 caagtccgac accgagatcc tgagcggcct gtggctctcg ctgcggatcg cgttcttcac
     1441 cgcctgcgcg tcggtcgtgc tcggcacgct ggcggcgctg gcgctcgtca agcaccggcg
     1501 cttcatcggc cgcacgctgt tctcgggcat gctgaacgcg ccgctggtga tgcccgaggt
     1561 gatcatcggc ctgtcgctgc tgctgatgct ggtggcgatg cagcaagcac tcggcttccc
     1621 cgagcgcggc ctgctgacga tctggctcgg ccacctgctg ctcggcatgg cgtacgccgc
     1681 cgtcgtcgtg caggcgcgcc tgaaggacct gaacccgcag ctcgaggagg cggcgatgga
     1741 cctcggcgcg cggccttcgc aggtgttctg gctggtcacg ctgccgatga tctcgcaggc
     1801 gctgctgtcg gcctggctgc tgaccttcac gctctcgctc gacgacgtgg tgctgtcggc
     1861 attcctgtcc ggccccggcg cgacgacgat gccgctggtg atcttctcgc gcgcccgcct
     1921 cggcctgaac ccgagcgtga acgcagtcgc gacgctgacg gtgctgctgg tgtcgatcgg
     1981 cgtgatcggc gcgagcctgt ggatcgcgcg gcgcgagcgc gcgcgcgccg ccgagatcgc
     2041 ggccgccgcg cgcgcggcat gacggcggca cagcgcccgc acgcaccctg gctctcgttc
     2101 ctgtgcctgg cgggcagcat ggcgctggtc ggcagctatg tcgggctgtc gaaggtgctg
     2161 gtgctggtgt tcccggtctt cctgctggcc ttcctgcgct tcggcatcgc ggcgttggcg
     2221 atggccggct gggtccggcg cggcgcgacc gagccggcgc tgaacgcgca cgaccgccgg
     2281 ctgctgttcc tcgaatcgtt cttcggcaac ttcctgttct cgatctgcat gctgttcggc
     2341 gtctcgatga gctcggcgct ggcagccggc gtgatcatgg ccggcatccc ggcggcggtg
     2401 gcgctgctgt cgcgcgtgtt cctgcacgag cgcatcgcgg cgcgcacgat gctgggcatc
     2461 gcctgcgcgg tcggcggcat cgcgctggtg tcgctgacga agaacgccgc cgcgccggcg
     2521 cacagctcgc tgctcggcaa cctgctgctc gtcgccgcgg tgctgtgcga ggccagctat
     2581 gtcgtgatcg gcaagaagct caccggcaac gtcagcccga agcgcatcag cgcgctgatc
     2641 aacctgtggg gcctcgcgct ggtcacgccg ttcggcgtct ggcaggcatt gagcttcgac
     2701 ttcggcgcgg tcgcggcggc gtcgtggtgg ctgctcgcgt tctactcgat cgcggccagc
     2761 atggtcaccg tgtggctgtg gatgaccggc ctgaagcacg tgcctgcctc gaaggccggc
     2821 atcttcacgg tgctgcttcc ggtcagcgcc gcggcggtcg gcgtgctgtt tctcggcgag
     2881 cgcttcagca cggcgcaggc ggcggcgttt gcgctcgcgc tggccggcgt ggtgctggcg
     2941 acctggccgt cgcgcaactc gaactgatca ggcgaaggct tcggcgcttt cgcgcgccag
     3001 ccggctgacc tcggccggat cggcctgctt caagcgatgg tccgaccaca cctgccgcca
     3061 gcggcgcgcg cccggcaggc cgttgcgcag gccgagcatg tggcgcgaca cgtgcgacca
     3121 cggctcgccg ccggcgacca cgcgctgcat gtacgcgacc atctgcgcct cgatcgcgtc
     3181 gcggtcgagc tcgaccggcg gcgcgccgaa gaagcgcgcg tcccagtccg ccatcagcca
     3241 cgggtggtgg tacggctcgc gacccagcat cacgccgtcg agcagctgca gctgttcggc
     3301 gatctgggcg ttcgtcttga tgccgccgtt cagcacgatc gtcagcgccg ggaactcgcg
     3361 cttgagccgg tgcacgagtt cgtagcgcag cggcggcagt tcgcggttct ccttcgggct
     3421 gatgcccttc agccaggcat tgcgcgcatg cacgatgaac acctcgcagc cggcatcggc
     3481 cacggtgccg acgaaatcgc gcacgaaggc gtagctctcg gtcttgtcga taccgatgcg
     3541 gtgcttcacg gtgaccggca ggctcaccgc atcgcgcatc gccttgacgc agtcggcgac
     3601 gagctgcggc tcggccatca ggcacgcgcc gaacgcgccg cgctgcacgc gctcgctcgg
     3661 gcagccgcag ttcaggttga cctcgtcgta gccccactgc ccggccagct tcgcgcagtg
     3721 cgccagatcg gccggctcgc tgccgccgag ctgcagcgcg agcgggtgct cggcggcgtc
     3781 gaagtccagg tgacgcggca catcgccatg cagcagcgcg ccggtcgtga ccatctcggt
     3841 gtacagatgc gtgtgccggc tcagcaggcg atggaagaag cggcagtgcc gatcggtcca
     3901 gtccatcatc ggggcgacac tcaggcgcca ccggttgaaa acttgttgct cgccgggttg
     3961 catagggctg cattttcccc cgagtgctcg ctcttcaccg cgtcgcggca agtggagttc
     4021 aatgctgtca ttgacatcat tgactacaat gccctatagc cggagaccgc aaccatggcc
     4081 acgctgacga tccgcaacct cgaagacgcg ctgaagagcc gcctgcgcct gcgcgccgcg
     4141 gcccgcaacc gctcgatgga ggacgaggtg cggcacatcc tgcgcgccgc gctgcaggag
     4201 cccgccgcgc ccgcgtccga cctgggcacg cgcatccgcg ctcgctttgc cggcctgggc
     4261 gacgtgcagc tgccggtcac cgcgcgcgag cccgtgcgac cgccgccgct gttcgacgcc
     4321 gcgccggcaa agagcgcgcg ccgcaagtga gtacgctgct cgacaccaac gtgctctccg
     4381 agttgctgcg cgcggcgccc gagccggcgg tgctgcgctg gctcgccgcc cagccggccg
     4441 acggcctatt cgtcaccgcc gtcacgcagg ccgagatgct gctcggcgcg cgcctgctgc
     4501 ccgccggcaa gcgacgcagc gcgctcgaag cggcgctgcg cggcctgttc gacgaagatt
     4561 tcgcgggccg cgtgctgccg ttcgacagcg gcgcgacgcc ggcctatgct gaactcgtcg
     4621 ccgcaaggcg cgccgcgggc cggccgatct cgcagttcga tgcccagatc gccgcgatcg
     4681 cacgtcgcca cgcgatgggc gtcgcaacgc gcgatgtcga cgactt
//
LOCUS       DASRDG010000664         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1487067, whole genome shotgun sequence.
ACCESSION   DASRDG010000664 DASRDG010000000
VERSION     DASRDG010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487067"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2828)
                     /locus_tag="VFA35_11125"
     CDS             complement(<1..>2828)
                     /locus_tag="VFA35_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZT56772.1"
                     /translation="GLVISAIGALALARGMVRPIRTLDDGARRIGAGNLDQKIEIHTN
                     DELQTLAERFNRMSGALRESYAGLERKVEERTRELQNSLEQQTAISEILRVISSSPTD
                     VQPVLDAVAKRAGLLCRADGSRVWLAVDGQLRAMTSYGEAYGTDSLNEVLPVRRSSIG
                     GRAFMEHRSVHVEDVVPLLDTEYPDVRALQARYGFRTVLVVPMLREGRSIGIIALLRN
                     QVRPFTPVEIALVQTFADQAVIAIENVRLFNETREALEQQTATAQVLQVISSSVADAR
                     PVFDEILRSCQRLFDSAEQGILLVDADGRAHLGAHHGSAQPLLQQIFAGGVPIAQFGD
                     GGRRRTPLHVPDVFADGAPGWLRQIGERLGVGAYSQVLAPIAREGRLVGYLYAIRQPA
                     TGFDAKEIALLQTFAEQAVIAIENARLFNETREALHEVEQRTAELSEALEYQTAISEV
                     LRVISTSPTDVAPVFEAILDSASRLLGTPLSAVFRYDGTLVHQVAVRNWPAAAVADAR
                     RFYPGPPNPQMMSGRAILSGEVQVEEDTLADTGYDQASARLGLWRRMVAAPLLRDGIA
                     VGAIVLAWREPGVTPQRQVELLKTFADQAVIAIENVRLINETKEALEQQTATAEVLQV
                     ISRSPTDVQPVFDAICERAMTLCGARIGGVARFDGELVHLVAFHGASPEATQAMLAAF
                     PMKPGRGAITARVILERAPVQIPDVLADPDYALQDAARRSGYRSNLGVPMLREGQVVG
                     SIGICREEPGPFPDKQVKLLQTFADQAVIAIENVRLFNETKEALEQQTATAGVLQVIS
                     NSVADTQPVFDAIVGSCRQLFGGRNVILLLAAGATLKRVAFATDGTLVTEGEWEWPLD
                     RTSASGDCVVSSKVIAVRDIDEVAARYPRTPQLAKRSHWVSGLFVPLRREGGAIGCIG
                     ILRGVQGEFSAKEIALAQTFADQAVIAI"
BASE COUNT          399 a         1009 c          996 g          424 t
ORIGIN      
        1 gatcgcgatc acggcttggt cggcgaaggt ctgcgccagc gcgatctcct tggcggagaa
       61 ctcgccctgc acgccgcgca ggatgccgat gcagccgatc gcgccgccct cgcgccgcaa
      121 gggcacgaac aggcccgaga cccagtggct gcgcttcgcg agctggggcg tgcgcgggta
      181 gcgcgcggcg acctcgtcga tgtcgcgcac cgcgatcacc ttcgacgaga ccacgcagtc
      241 gcccgaggcg ctcgtgcggt cgagcggcca ctcccactcg ccttcggtga cgagcgtgcc
      301 gtcggtcgcg aacgcgaccc gcttcagcgt cgcacccgcg gcgagcagca ggatcacgtt
      361 gcgcccgccg aagagctgcc ggcaactgcc gacgatggcg tcgaagaccg gctgcgtgtc
      421 ggcgaccgag ttgctgatga cctgcagcac gccggcggtg gcggtctgct gctccagcgc
      481 ctccttggtc tcgttgaaca gccgcacgtt ctcgatcgcg atcacggcct ggtcggcgaa
      541 ggtctgcaac agcttcacct gcttgtcggg gaacgggccg ggttcctcgc gacagatccc
      601 gatcgagccg acgacctggc cctcgcgcag catcggcacg ccgaggttgc tgcggtagcc
      661 cgaccgccgg gcggcgtcct gtagcgcgta gtcggggtcg gcgagcacat cggggatctg
      721 gaccggcgcg cgttcgagga tcacgcgcgc ggtgatcgcg ccacgcccag gcttcatcgg
      781 aaaggccgcg agcatggcct gcgtcgcctc cggcgaggcg ccgtggaaag cgaccaggtg
      841 caccagttcg ccgtcgaagc gcgcgacgcc gccgatgcgc gcgccgcaca gggtcatcgc
      901 gcgttcgcag atcgcgtcga acaccggctg cacgtcggtc ggcgagcgcg agatcacttg
      961 cagcacctcg gcggtcgcgg tctgctgctc cagcgcctcc ttggtttcgt tgatcaggcg
     1021 cacgttctcg atcgcgatca cggcctggtc ggcgaaggtc ttgagcagtt cgacctggcg
     1081 ctgcggcgtc acgcccggct cgcgccacgc cagcacgatg gcgccgacgg cgatgccgtc
     1141 cctgagcaag ggcgcggcga ccatgcgccg ccacaggccg agccgcgccg aggcctggtc
     1201 gtagccggtg tcggcgaggg tgtcctcctc gacctgcacc tcgcccgaga ggatggcgcg
     1261 gccgctcatc atctgcgggt tgggcgggcc tgggtagaag cgccgcgcgt cggcgacggc
     1321 ggcggccggc cagttgcgca cggcgacctg gtgaaccagc gtgccgtcgt agcggaacac
     1381 cgccgacagc ggcgtgccga gcagcctcga ggcactgtcc aggatcgctt cgaacaccgg
     1441 cgccacatcg gtcggcgagg tgctgatgac gcgcagcacc tcgctgatgg cggtctggta
     1501 ctcgagtgct tcgctcaact cggcggtgcg ctgctcgacc tcgtgcagcg cctcgcgggt
     1561 ctcgttgaac aaccgcgcgt tctcgatcgc gatcaccgcc tgctcggcga aggtctgcag
     1621 cagcgcgatc tccttcgcgt cgaagccggt cgccggctgg cggatcgcgt acaggtagcc
     1681 gaccaggcgg ccctcgcgcg cgatcggcgc cagcacctgc gaatacgcac cgacgccgag
     1741 ccgctcgccg atctgccgca gccaccccgg cgcgccgtcg gcgaacacgt cgggcacatg
     1801 cagcggcgtg cgccggcgcc cgccgtcgcc gaactgcgcg atcggcacgc cgccggcgaa
     1861 gatctgctgc agcagcggct gcgccgagcc gtggtgggcg cccaggtgcg cccggccgtc
     1921 cgcgtcgacg agcaggatgc cctgctcggc gctgtcgaac aggcgctggc agctgcgcag
     1981 gatctcgtcg aacaccgggc gcgcatcggc caccgacgag ctgatgacct gcagcacctg
     2041 cgccgtcgcg gtctgctgct cgagcgcctc gcgcgtctcg ttgaacaggc gcacgttctc
     2101 gatcgcgatc acggcctggt cggcgaaggt ctgcaccagc gcgatctcga ccggcgtgaa
     2161 cgggcgcacc tggttgcgca gcagcgcgat gatgccgatc gagcggccct cgcgcagcat
     2221 cggcacgacc agcaccgtgc ggaagccgta gcgcgcctgc agcgcgcgca cgtcggggta
     2281 ctcggtgtcg agcagcggca cgacgtcttc gacatgcacg ctgcgatgtt ccatgaacgc
     2341 gcggccgccg atcgagctgc gccgcaccgg cagcacttcg ttcagtgaat cggtgccgta
     2401 ggcctcgccg tagctcgtca tcgcgcgcag ctgcccgtcg acggcgagcc agacgcggct
     2461 gccgtcggcc cggcacaaca gcccggcgcg cttggcgacc gcgtcgagca ccggctgcac
     2521 gtcggtgggc gagctcgaga tcacgcgcag gatctcgctg atggcggtct gctgctcgag
     2581 cgagttctgc agttcgcgcg tgcgctcctc caccttgcgc tcgaggcccg cgtaggattc
     2641 gcgcagcgct ccgctcatgc ggttgaagcg ctcggccagc gtctgcagct cgtcgttggt
     2701 atggatttcg atcttctggt cgagattccc cgcgccgatg cgccgcgctc cgtcgtcgag
     2761 cgtgcgtatc gggcgcacca tgccgcgggc gagcgcaagc gcgccgatgg ccgaaatcac
     2821 gaggccgg
//
LOCUS       DASRDG010000665         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_105264, whole genome shotgun sequence.
ACCESSION   DASRDG010000665 DASRDG010000000
VERSION     DASRDG010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105264"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFA35_11130"
     CDS             complement(<1..188)
                     /locus_tag="VFA35_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56773.1"
                     /translation="MHDARDAAAVLGLHQQHVAAVALGHDLILQVFRGFLAAQVRLER
                     AAQPCFLLSQAIADQPQLR"
     gene            complement(195..473)
                     /locus_tag="VFA35_11135"
     CDS             complement(195..473)
                     /locus_tag="VFA35_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56774.1"
                     /translation="MARALQFEGAVRRERRLDAVDRLGEARPPLQHVELGRRADRQLQ
                     IVRAPTERVGQREQNAPDFLGLLLFERHDVVVDFHRAERLEKQAGAAG"
     gene            537..1937
                     /locus_tag="VFA35_11140"
     CDS             537..1937
                     /locus_tag="VFA35_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZT56775.1"
                     /translation="MTGPNTGGKTVALKSAGLLAMMMQAGLRIPAEHGSRLPVFRSLF
                     ADIGDEQSIEANLSTFSAHIKNIASMDRGLVTPALVLLDEVGSGTDPIEGGALGVAIV
                     DHFRRRGATVIATSHYDQLKTYASTTAGVSSAAFGFELATFAPTYQLIYGSPGRSLAL
                     EIAGRLGLNPSVVAAARENLTAREAQLAEHLAKIDHDLRALEHEHRLAARERDTLKEA
                     ETRMHQREDALRQREETFKKRLNEELAAQVRQARKEIDEVIAALKAKAEAIAHGPRLV
                     STGDTGKARSDARAAVDAITARTSDQSTIDNSQSISNPQSAISNVSVGSRVIVGGLGL
                     EATVTSVHDGTADLDVRGKRMRASVRDLRVIDGQPAAARVNVNVELQPRETTSADLNV
                     IGCTVDEALTRAERFLDESLLGDQRVLRLIHGYGTGQLKRALAGFLQQHPLVARFATA
                     PPNQGGGGVTVVELKD"
     gene            1937..>3055
                     /gene="dnaG"
                     /locus_tag="VFA35_11145"
     CDS             1937..>3055
                     /gene="dnaG"
                     /locus_tag="VFA35_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZT56776.1"
                     /translation="MGLFPQQFIDDLRMQASILTVVQEYVPLKRAGTSYKGNCPFHNE
                     KTPSFNVNPEKGFFHCFGCGVGGDVFKFLELHEKVGFQDAVKMLAQKFGVALPELAPG
                     EGGDDARRDSALREGLLKAHEIAAAYFAEQLAAPAGGRARSQLAERGVAPQTIAQLGL
                     GFAPNSRDGLKQRLLKQGFAQGVLLQSGLVVQREGGEVVDRFRNRLMVPICRDTGSVI
                     AFGGRQMDPDQGGPKYLNSPETPIYSKGRTLYGLNLTKGQIRQGGFAVLVEGYFDFAQ
                     VFQSQAAPTVASCGTALTPQQAQLLRRFTSKIVLSFDPDAAGLGAAVRSCELLVAEGF
                     DVNVVVLDKGEDPDTFIRRKGGDQYRDRLKSSRPYLEYL"
BASE COUNT          545 a         1017 c         1029 g          464 t
ORIGIN      
        1 cggagctgcg gctgatccgc gatcgcctgc gaaagcagaa aacacggctg cgcagcacgc
       61 tcgagtcgta cctgcgcggc aaggaaaccg cgaaatacct gcaggatcag gtcgtgtccg
      121 agcgcaacgg ccgctacgtg ctgctggtga aggccgagca ccgcggcggc atcccgggca
      181 tcgtgcacgg cgcctcagcc agcggcgcca gcctgtttct cgagccgctc agcacggtgg
      241 aaatcaacaa cgacatcgtg gcgctcgaag agcagcaggc cgaggaagtc cggcgcattc
      301 tgctcgcgct gaccgacgcg ttccgtcggc gcgcggacga tctggagctg acgatcggcg
      361 cggcgaccga gctcgacgtg ctgcaggggc gggcgcgctt ctccgagtcg atcgacggcg
      421 tcgagccggc gctctcgacg gacggcgcct tcgaactgca gggcgcgcgc catcctctgc
      481 tcaagaaccc ggtcgcggtg acgatcaagg tgattccacc ggcgaccatc ctgctggtga
      541 ccggaccgaa caccggcggc aagaccgtcg cgctgaagag cgccgggctg ctggcgatga
      601 tgatgcaggc cggcctgcgc atcccggccg agcacggatc gcggctgccg gtgttccggt
      661 cgctgttcgc cgacatcggt gacgagcagt cgatcgaggc gaatctcagt accttctcgg
      721 cgcatatcaa gaacatcgcg tcgatggacc gcgggctggt gacgcccgcg ctggtgctgc
      781 tggacgaagt ggggtctggc accgatccga ttgaaggcgg cgccctcggc gtggcgatcg
      841 tggatcactt ccgccgccgc ggtgcgaccg tgatcgcgac cagccactac gaccagctca
      901 agacctacgc ctcgaccacc gccggtgtgt ccagcgcggc gttcgggttc gagctggcca
      961 cgttcgcgcc gacctatcag ctgatctacg gcagccccgg ccgcagcctg gcgctcgaga
     1021 tcgccggccg gctcggactg aacccgtcgg tggtcgccgc tgcgcgcgag aacttgaccg
     1081 cgcgcgaagc gcagctcgcc gagcatctcg ccaagatcga ccacgacctg cgcgcgctcg
     1141 agcacgagca tcggctcgcg gcgcgcgagc gcgacacgct gaaggaggcc gagacgcgca
     1201 tgcaccagcg cgaggacgcc ctgcgccagc gcgaagagac gttcaagaag cggctcaacg
     1261 aagagctcgc ggcccaggtg cgccaggcgc gcaaagagat cgacgaagtg atcgcggcgc
     1321 tgaaggccaa ggccgaggcg atcgcgcacg gcccgcgcct ggtctccacc ggcgacaccg
     1381 gaaaggctcg cagtgacgcg cgcgccgcgg tcgacgcgat caccgcgcgc acgagcgatc
     1441 aatccacaat cgacaattca caatcaatca gcaatccgca atccgcaatc agcaatgtgt
     1501 ccgtcggcag ccgagtcatt gtcggtggcc tcgggctcga ggcgacggtg acctcggtgc
     1561 acgacggcac cgccgatctc gacgtacgcg gcaagcggat gcgcgccagc gtccgcgacc
     1621 tgcgcgtcat cgacggccag ccagccgccg cgcgcgtcaa tgtcaacgtc gagctgcagc
     1681 cgcgcgagac gacctcagcc gatttgaacg taatcggctg caccgtcgat gaagcgctca
     1741 cgcgggcgga gcgctttctc gacgagtcgc tacttggcga tcagcgcgtg ctgcggctga
     1801 ttcacggcta cggcacgggg cagctgaagc gcgcgctcgc aggcttcttg caacaacacc
     1861 cgttggtcgc gcggttcgcg acggcgccgc ccaatcaggg cggcggcggc gtcaccgtcg
     1921 tagagttgaa ggactagtgg gcctgttccc gcagcaattc atcgacgacc tgcgcatgca
     1981 ggccagcatc ctcacggtgg tgcaggaata tgtacccctg aagcgcgcgg ggacgtctta
     2041 caagggcaac tgcccgttcc acaacgaaaa gaccccgtct ttcaacgtga accccgagaa
     2101 ggggttcttc cactgtttcg gctgcggcgt cggcggcgac gtgttcaagt ttctcgagct
     2161 gcacgagaag gtcggcttcc aggacgcggt gaagatgctc gcgcagaagt tcggcgtggc
     2221 gctgcccgaa ctggcccccg gcgagggggg cgacgatgcc cgccgcgatt cggcgctgcg
     2281 cgaaggcctg ctcaaggcgc acgagattgc ggcagcctac ttcgccgagc agctggccgc
     2341 accggccggc gggcgcgccc ggtcgcagct cgccgagcgc ggcgtcgcac ctcagaccat
     2401 cgcccagctc ggcctcggat tcgcgcccaa ttctcgcgac ggcttgaagc aacggctcct
     2461 gaaacaaggc ttcgcgcagg gcgtattact gcaaagcggc ctggtcgtgc agcgcgaggg
     2521 cggcgaggtg gtcgatcggt tccgcaatcg gctgatggtg ccgatctgcc gagataccgg
     2581 ctcggtgatc gccttcggcg gccgtcagat ggatccggac cagggcgggc cgaaatatct
     2641 caattcgccg gaaacgccca tctattcgaa gggccgtacg ctctacggtc taaatctgac
     2701 caaaggccag atccgacaag gcggcttcgc ggtcctggtg gaaggctatt tcgacttcgc
     2761 gcaggtgttt cagtcacagg cggcccccac ggtcgcctcc tgcggcacgg cgctgacgcc
     2821 gcagcaggcg cagctgctcc ggcggttcac gtcgaagatc gtgctcagtt tcgaccccga
     2881 cgcggccggc ctcggcgcgg cggtccgttc atgtgagttg ctcgtggcgg aggggttcga
     2941 cgtcaacgtc gtggtcctcg acaagggcga ggatcccgat acattcatca ggcgcaaagg
     3001 cggcgatcaa tacagggacc ggctgaaatc gtcgcggccg tatctcgaat acctg
//
LOCUS       DASRDG010000666         5479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1256579, whole genome shotgun sequence.
ACCESSION   DASRDG010000666 DASRDG010000000
VERSION     DASRDG010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256579"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFA35_11150"
     CDS             <1..567
                     /locus_tag="VFA35_11150"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HZT56777.1"
                     /translation="PGLELPMHTRSGHWQIAAAAVAIGFVLSGHGQWAAVPLTLAATI
                     GLAQVLAWQPWAARHKPILWILHAGYALLNAGLLLAAVRASGQPLRAAWPIHLIAMGG
                     LSVLIIGMITRTALGHLGRPLLIDRWMLASFWLMLAAVVLRLLALLPFSVHGLLEGAA
                     VCWIAAFAIYIERFASLMIRPRADASKS"
     gene            690..2351
                     /locus_tag="VFA35_11155"
     CDS             690..2351
                     /locus_tag="VFA35_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZT56778.1"
                     /translation="MDSAIMSLLGAFVLSIIGLFVFVWSLRQGLLVENPVAASVIFAP
                     GELGKVDDPALTEKAQRSMQGAVGARDAPIPAVDAVDVATIEARVASDQSSAFPVFMF
                     IAFACMWLLLGSTAGLVSSIKLHAPDWLVSEAWMTFGRMRSVHLTAVLYGWITNAALG
                     MILWVLPRLLGTRLLGGIWAMLGGALINTGIAGGIGAIGTGWSDGMEYLEIPWQFAIF
                     IFVGFVLVILPVLFTLVNRKVEHLYVSVWYMVAALLWIALLFLVGKLPGVHTGVQQAT
                     TNWWYGHNVLGLWFTPVSVGAIYYFLPKIIGRPVSSYNLSILGFWTLAFFYGQVGGHH
                     LIGGPVPGWLTTLSIVQSVMMIIPVIAFSINMGGTLRGRMHLARYSPTLRFMMFGGLM
                     YMASSLQGTLESLRSVNQVTHFTHFTVAHAHLGAYGFVTMVLFGAIYFMMPRVLNWEW
                     PYPKLITLQFWLAAIGILVYFIGLSIGGWLQGEAMLDAKRPFMESVTLMAPYLFSRSV
                     GGSLMLLSHLIFVFHFLAMALRFGPTRTSAALFWQQGLQERANGQ"
     gene            2341..2931
                     /locus_tag="VFA35_11160"
     CDS             2341..2931
                     /locus_tag="VFA35_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HZT56779.1"
                     /translation="MDNEVKLAAGTMVALSLATAALVVLPYIQVRDIKPLPGLKAYTS
                     EQLRGRNVYVANGCVYCHTQQPRDRNFGPDLERGWGRASVPADYVYDRPHLLGSMRTG
                     PDLFNIGARQPSKDWHLGHLYQPRAYVPGSIMPAYPYLFYVKVAAEEGEQVLNLPPGY
                     SPAGKVVVPTPEALDLVKYLQALDHTYPVLPPEPKP"
     gene            3051..3563
                     /locus_tag="VFA35_11165"
     CDS             3051..3563
                     /locus_tag="VFA35_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZT56780.1"
                     /translation="MITLAVVLTGVLYILLSEPFGRPELGDRRTLADLRPAVVRPGQG
                     ADGQQVYSANCVACHQATGKGLPGVFPPLDGSEWVNGDPRILANIVLHGVTGDISVAG
                     QVYKGAMPSFAQLGDAELAAVLSHVRSAWSNKSAAIKSGLIATERKASERKTPFAGGD
                     ELKTLTGKTP"
     gene            3566..4027
                     /locus_tag="VFA35_11170"
     CDS             3566..4027
                     /locus_tag="VFA35_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZT56781.1"
                     /translation="MRLTMMSDYALRLLMYAAQQPDRLCTIAEVAQAYAISEAHLMKV
                     THQLAVAGWLVTVRGKGGGLRLARPAGEINLGAVVRDIEPDFFLVECFASGSACTLTG
                     RCRLTGVLQNALQGFMDGLDRHTLADIVPPATLAAKPGTRKRSPVVRMRVA"
     gene            4027..4656
                     /locus_tag="VFA35_11175"
     CDS             4027..4656
                     /locus_tag="VFA35_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZT56782.1"
                     /translation="MLRTALASLSLALLGWWSAAWITEDFQVWTAEGARRLTVIEQPV
                     PAPKVALSGPGMIDADLHTFLAGADRATIVDFVYTRCPTVCTTLGSGFQQLQQALAAQ
                     QAEGVKLLSISFDPAHDDAAGLQRYAAQWRADPKRWAVATVPDVDQLQRLLKAFQVVV
                     IDDGRGGYEHNAALLVLDGRGRLVRIFDDTELELALNYARSIARRGAGK"
     gene            4767..5306
                     /locus_tag="VFA35_11180"
     CDS             4767..5306
                     /locus_tag="VFA35_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56783.1"
                     /translation="MAAHMLIQYPCLILAGALLVERVPNRWLEVAQRWNELGIAGLVC
                     SALTLAVLMIPRVLDLALVDGQVETTKLIALIASGAALRLSWKRAGVVVQTFFLGNVL
                     PMTGVAGTMYQDSAVRLCNAYRLDDQHDLGLALVVIAIATAVVWLLIVATRRSGGDST
                     VPCEQTMVKAASREPGPRP"
BASE COUNT          895 a         1778 c         1774 g         1032 t
ORIGIN      
        1 cccggtctcg agctcccgat gcacacgcgc agcggtcatt ggcagattgc cgcagcggcg
       61 gtcgcgatcg gcttcgtgct ttctggccac gggcaatggg cagccgttcc gttgacgttg
      121 gcggccacca tcggcttggc ccaggtgctg gcgtggcagc cctgggcggc gcggcacaaa
      181 ccaatcctct ggatactcca cgccggttac gccctcctga atgcgggcct gctgctggca
      241 gcggtccgcg catccggtca gccactgcgc gcggcctggc cgattcacct gatcgcgatg
      301 ggtgggctgt cggttctgat catcggcatg atcacgcgca ccgcgctcgg ccacctgggg
      361 cgaccgttgc tgatcgatcg ctggatgctc gcgagtttct ggctcatgct tgcagccgtg
      421 gtgcttcgac tgctggcatt gctcccgttc tcggtgcacg ggttgctcga aggcgcagcg
      481 gtgtgctgga tcgctgcgtt tgcgatctac atcgagcggt ttgcttcgct gatgatccgg
      541 ccgcgtgccg atgcgtcgaa gagttgaatg cgggcgagca acagggccga catgtccaca
      601 gcaagctatg acaagacgcg gagcgggatc aggatgcaca ctcccgaccc cggcgggccg
      661 cgaatcgcgg catcacaaga ggtgaactga tggacagtgc aatcatgagt ctgctcggcg
      721 ctttcgtgct gtcgatcatc ggcctgttcg tcttcgtgtg gtcgctgcgc cagggccttc
      781 tggtcgagaa ccccgtcgcc gcctccgtca tttttgcccc tggcgagctc ggcaaggtcg
      841 acgatccggc gctcaccgag aaggcgcagc gttcgatgca gggcgcagtc ggcgcacgcg
      901 atgccccgat cccagcggtc gacgcggtcg atgtggcgac catcgaagcg cgggtcgcct
      961 cggatcagtc gtccgccttc ccggtgttca tgttcatcgc gttcgcctgc atgtggttgc
     1021 tgttgggttc gaccgccggc ttggtctcgt cgatcaagct gcatgcgccc gactggctgg
     1081 tgagcgaagc ctggatgacc ttcggccgca tgcgcagcgt gcacctcacc gcggtgctgt
     1141 acggctggat cacgaacgct gcgctcggca tgattctctg ggtgctgccc cgcctgctgg
     1201 gcacgcgcct gctcgggggc atctgggcca tgctcggcgg cgcgttgatc aacaccggca
     1261 tcgccggcgg catcggcgcg atcggcaccg gctggagcga cggcatggag tacctcgaga
     1321 ttccgtggca gttcgcgatc ttcatcttcg tcggcttcgt gctcgtgatc ctgccggtgc
     1381 tgttcacgct ggtgaaccgc aaggtcgagc acctgtacgt cagcgtctgg tacatggtcg
     1441 cggcgctgct ctggatcgcg ctgctgttcc tggtcggcaa gctgccgggc gtgcacaccg
     1501 gggtgcagca ggccacgacc aactggtggt acgggcacaa cgtgctgggc ctgtggttca
     1561 cgccggtcag cgtcggcgcg atctactact tcttgcccaa gatcatcggg cgcccggtca
     1621 gctcgtacaa cctgtcgata ctcggcttct ggacgctcgc gttcttctac gggcaagtcg
     1681 gcggccacca cctgatcggc ggcccggtgc ccggttggct gaccacgctg tcgatcgtgc
     1741 agagcgtgat gatgatcatt ccggtcatcg ccttcagcat caacatgggc ggcacgctgc
     1801 gcggtcgcat gcacctggcg cgctactcgc cgaccttgcg cttcatgatg ttcggcggcc
     1861 tgatgtacat ggcgtcgtcg cttcaaggca cgctcgaatc gctgcgcagc gtgaaccagg
     1921 tgactcactt cacgcacttc acggtcgcgc atgcgcatct gggcgcctac ggcttcgtca
     1981 cgatggtgct gtttggtgcg atctacttca tgatgccgcg cgtgctcaac tgggagtggc
     2041 cctatccgaa gctgatcacg cttcagttct ggctcgcggc gatcggcatc ctggtctatt
     2101 tcatcggcct ctcgatcggg ggctggctgc agggcgaggc gatgctcgac gcgaagcggc
     2161 ccttcatgga gtctgtcacg ttgatggcgc cctatctctt ctcgcgctcg gtggggggat
     2221 cgttgatgtt gctcagccac ctgatcttcg tcttccactt cctcgcgatg gcgctgcgtt
     2281 tcgggccgac acgcaccagt gctgcgctgt tctggcaaca gggccttcag gagcgcgcga
     2341 atggacaatg aagtcaagct cgcagccggc accatggtgg cgctgagcct ggcaacggca
     2401 gcgctggtgg tgttgcccta catccaggtg cgcgacatca agccgctgcc cggcctcaag
     2461 gcatacacca gtgagcaact gcgagggcgg aacgtctacg tggccaacgg ctgcgtgtac
     2521 tgccataccc agcagccgcg cgatcggaac ttcggccccg acctcgaacg cgggtggggc
     2581 cgcgcctccg tgccggccga ctacgtgtac gaccggcctc acctgctcgg cagcatgcgc
     2641 accggccccg acctgttcaa catcggcgca cgccagccca gcaaagactg gcatctcggc
     2701 cacctgtacc agccgcgcgc ctacgtgccg ggttcgatca tgccggccta cccttatctt
     2761 ttctacgtga aggtagccgc agaagaaggc gaacaggtcc tcaacctgcc gccgggctac
     2821 agccccgctg gcaaggtggt ggtgcccacg cccgaggcgc tcgacctggt caagtacctg
     2881 caggcgctcg accacaccta cccggttctg ccccccgagc cgaagccctg acgctgattc
     2941 aaagcaaggc cgacaccatg tcaaatgaat ccgaaccggg ccgccgcgcg caacagcgcg
     3001 agacgaccga gccgtccgaa cgcagccagc cgattccatt gatagttgcg gtgatcacgc
     3061 tcgcggtggt gctgaccggc gtgctctaca tcttgctgtc ggagccgttc ggccggcccg
     3121 aactcggcga ccgtcgcacg ctggctgacc ttcgaccggc cgtcgttcgc cccggccagg
     3181 gcgccgacgg gcagcaggtc tacagcgcca attgcgtcgc ttgccatcaa gcgacgggca
     3241 aggggttgcc tggcgtgttc ccgccgctcg acggttcgga atgggtcaac ggcgatccgc
     3301 gcatcctggc caacatcgtg cttcacgggg taacgggcga catcagcgtc gcaggtcagg
     3361 tttacaaggg ggcgatgccg agcttcgcgc aactgggcga cgccgagctg gcggcggtgc
     3421 tcagccatgt tcgcagcgca tggtccaaca aatctgccgc gatcaagagc ggtctgatcg
     3481 cgaccgaacg caaggccagc gaacgcaaaa cgccgttcgc gggcggcgac gagttgaaga
     3541 cgctgaccgg aaagacgccc tgatcatgcg gttgaccatg atgtcggact acgcgctgcg
     3601 gctgttgatg tatgcagcgc aacagccgga ccgcctctgc accatcgccg aagtggcaca
     3661 ggcgtacgcc atctctgaag ctcacttgat gaaagtcacg caccagctcg cggtggcggg
     3721 ctggctcgtt accgtgcgag gaaaaggcgg cgggctgcgt ttggcaagac cggcgggcga
     3781 gatcaacttg ggcgccgtgg tgcgcgacat cgagcccgac ttctttctgg tcgagtgctt
     3841 cgcgagcgga agcgcctgca ccttgacggg ccgatgccga ctcaccggtg ttctgcaaaa
     3901 cgcgttgcaa gggttcatgg acgggctgga ccgccacacg ctcgccgaca tcgtgccccc
     3961 ggcaacactc gccgccaagc ctggaacacg caagcgttcg ccggtcgtgc gcatgcgcgt
     4021 ggcctgatgc tgcgaaccgc gctggccagt ctttccctgg cgttgctcgg ctggtggagc
     4081 gccgcctgga tcaccgaaga cttccaggtc tggaccgcag agggcgcacg ccgcctgacc
     4141 gtgatcgagc aacccgtgcc cgcgccgaag gtggcgctct caggccccgg catgatcgac
     4201 gcggacctgc acactttcct ggcgggcgct gacagagcca cgatcgtcga ttttgtctac
     4261 acacgttgcc cgacggtgtg caccacgctc ggcagcggat tccagcaact tcagcaggca
     4321 ctcgcggcgc aacaggccga gggcgtgaaa ctgctttcga tcagtttcga tccggcccat
     4381 gacgacgccg ccggcctgca gcgttatgcg gcccaatggc gcgccgaccc caagcgctgg
     4441 gcggtcgcca cagtgcccga cgtcgatcaa ctccagcgcc tgttgaaggc gttccaggtc
     4501 gtggtcattg acgacggtcg cggtggctac gagcacaacg cggcacttct tgtgctcgac
     4561 ggacgcggac gcctggtacg gatcttcgac gacaccgagc tcgaactcgc gctgaactac
     4621 gcgcgctcga tcgctcggcg aggggccggc aaatgacgtt gtcctcaggc tcggccgagc
     4681 gcgccgcaac gtggcgaacg gcagcggctt ggctcctgct gctcatgatg ctcgtgccac
     4741 ccttgcggaa aatgatcgag tccagcatgg ccgcgcacat gctgattcag tacccctgcc
     4801 tgattctggc aggcgcactt cttgtggagc gtgtgcccaa tcggtggctc gaggttgcgc
     4861 agcgctggaa cgaactgggc atcgcgggcc ttgtctgcag cgcgctcacg ctggcggttc
     4921 tgatgatccc gagggtgctg gacctggcgc tcgtggatgg gcaggtcgag acaacaaagc
     4981 tgatcgcact gatcgccagc ggcgcagcgc tgaggctgtc ctggaagcgc gcaggcgtgg
     5041 tggtgcagac attttttttg ggcaacgtgc ttccgatgac gggcgtggcc ggaaccatgt
     5101 accaagattc cgcggtgcgc ctgtgcaacg cctatcggct tgacgatcag cacgacctcg
     5161 ggctcgcgct cgtcgtcatc gcgatcgcga cggcggtggt ttggctactc attgtcgcca
     5221 cgcgccggtc gggcggcgat tccacggtcc catgcgagca aaccatggtg aaggcagcat
     5281 cgcgtgagcc tggaccgcgc ccatgagtgc ccctatcaag ttgtgcaggg acacctcgaa
     5341 ctggactcac catccgaggg gacgaaaccg atgaacatgg tcagcttgat acacagaatt
     5401 ctgcacaaac tgcaccggtg gtgagcgcat aagcgctaga tgccccgcat ccacagcggc
     5461 atttcgaagc gccgatccg
//
LOCUS       DASRDG010000667         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1365530, whole genome shotgun sequence.
ACCESSION   DASRDG010000667 DASRDG010000000
VERSION     DASRDG010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365530"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..472)
                     /gene="purU"
                     /locus_tag="VFA35_11185"
                     /pseudo
     CDS             complement(1..472)
                     /gene="purU"
                     /locus_tag="VFA35_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426395.1"
                     /note="produces formate from formyl-tetrahydrofolate which
                     is the major source of formate for PurT in de novo purine
                     nucleotide biosynthesis; has a role in one-carbon
                     metabolism; forms a homohexamer; activated by methionine
                     and inhibited by glycine; frameshifted; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
     gene            complement(547..1557)
                     /gene="waaF"
                     /locus_tag="VFA35_11190"
     CDS             complement(547..1557)
                     /gene="waaF"
                     /locus_tag="VFA35_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HZT56784.1"
                     /translation="MARTSSLVIAPQWIGDAVMSEPLLARLARRGEQLTVAALPWVAP
                     VYRAMPQVGAVIELPFAHGRLDWGARRRLAGELRGRFDTAYVLPNSIKSALLPWLAGI
                     PRRIGYQGEGRVLLLNKRLPNPPGRPPMVAFYAALSGEPAHEGERPLMKFDTSVLEQA
                     TRAAAVEPGAYWAFAPGAEYGPAKCWPADRYAELARTLHQQHGQPVLLLGSGKEATLC
                     EQIAAQSEGACRVLAGRTSLIDAMALIAAARGVVSNDSGLMHVAAAFGVPQAAVFGST
                     SPEHTPPLNPRARVLWLKQELQLDCAPCFDRVCRFGHTRCLSGVSAARVESALDDALS
                     AS"
     gene            complement(1544..1747)
                     /locus_tag="VFA35_11195"
     CDS             complement(1544..1747)
                     /locus_tag="VFA35_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HZT56785.1"
                     /translation="MSNPNEIVELLAKDLQGHGVAFCPNPKMALWSGHPRVFLDVGAV
                     GEAKCPYCGTVYRLKAGEVVGSH"
     gene            complement(1744..2667)
                     /locus_tag="VFA35_11200"
     CDS             complement(1744..2667)
                     /locus_tag="VFA35_11200"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZT56786.1"
                     /translation="MAMDDRDGKIWMDGTMVDWRDAKIHVLTHTLHYGCGAFEGVRAY
                     NTVDGTAIFRLREHTERLFNSAKILRMKIPFSFEEVVQAQLAVVRENKLESCYLRPLT
                     WIGSEKLGVSPKGNKVHLMVAAWPWGAYLGEEGLKRGIRVKISSYTRHHVNITMTQAK
                     AVSNYTNSILANMEATDDGYDEAMLLDANGFVSEGAGENLFVVKNGVVYTPDLSAGAL
                     NGITRNTVFAICADLGLKVVEKRITRDEVYIADEAFFTGTAAEVTPIRELDRIELGSG
                     SRGPITEKIQAAFFDIVNGRNPKYAEWLSKV"
     gene            2962..>3208
                     /locus_tag="VFA35_11205"
     CDS             2962..>3208
                     /locus_tag="VFA35_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56787.1"
                     /translation="MRFSKSLLGAAAALALIASVTAARADTIDYSFNVAANGLDVSGG
                     DYGTIHLATNGTGGVDFTITLRGDLNFTTTGNTGSHAL"
BASE COUNT          528 a         1090 c         1061 g          529 t
ORIGIN      
        1 ttggcctgcg cgctcgcgcc ggtcggcagc ggcaggtggt ggaacggcac gccgtgactc
       61 gcacacagtt cggcgaaatc ggggtggttc gagaccacgc ccgcaatcgc cgccggcagc
      121 tggcccgact ggcagcgaaa cagcaggtcg ttcaggcagt ggccgtgctt gctcaccatc
      181 agcagcaggc gcggctggcg cgccagtgca tgcaccttca ggtccatcgc gaagcgctcg
      241 cgcagcggcg cgaccaacgc ttccagcggc ggcgcgccgg ccagcgccgc cggcgcctcg
      301 aagtgcacgc gcatgaagaa caacccggtc gcgtcggcgc cgagcacgtc gccgaactgc
      361 tggagtcgat gatgttgcag ccggcctcgt acaacaggcc cgacaccgcg taaacgatgc
      421 ccttggtgtc tcgacaggag agggtgagga tgaattcgca ggggctgggc atgggggtag
      481 gtttcgctgc ctttttggcg ccttcgattg tacgaagccc tgcctcggcc agccgaggcc
      541 ggcgcctcag gaggcgctca acgcgtcgtc caacgccgac tcgacccgcg cggcgctgac
      601 gccgctcagg cagcgcgtgt ggccgaagcg gcagacgcgg tcgaagcagg gcgcgcagtc
      661 gagttgcagt tcctgcttca gccacagcac acgcgcgcgc gggttcagcg gcggcgtgtg
      721 ctcggggctg gtcgagccga acaccgccgc ctgcggcacg ccgaacgcgg cggcgacatg
      781 catcaggccc gagtcgttgc tgaccacgcc gcgcgccgcg gcgatcagcg ccatcgcgtc
      841 gatcagcgag gtccggcccg cgagcacgcg gcacgcgccc tcggactgcg cggcgatctg
      901 ctcgcacagc gtcgcttcct tgcccgagcc gagcagcagc accggctggc cgtgctgctg
      961 gtgcagcgtg cgcgcgagct cggcgtagcg gtcggcgggc cagcacttgg ccgggccgta
     1021 ttcggcgccg ggcgcgaacg cccaatacgc gccaggttcg acggcggcag cgcgggttgc
     1081 ttgttccagc accgacgtgt cgaatttcat cagtgggcgc tcaccttcat gcgccggttc
     1141 gccggacagc gcggcataga acgcgaccat gggcggccgc cccggcggat tcggcagacg
     1201 cttgttcagc agcagaaccc ggccctcgcc ttggtagccg atacgccgcg ggatgcccgc
     1261 cagccacggc agcagcgccg acttgatcga gttcggcagc acataggcgg tgtcgaagcg
     1321 accgcgcaac tcgccggcaa ggcgccgccg cgcgccccaa tcgagccggc catgggcgaa
     1381 cggcagctcg atcaccgcgc cgacctgcgg catcgcgcga tagaccggcg cgacccacgg
     1441 cagcgccgcg accgtgagct gctcaccgcg cctggcgagc cgcgccagca gcggctcgga
     1501 catcacagcg tcgccgatcc actgcggcgc gatgacgagg gagctagtgc gagccaacga
     1561 cctcgccggc tttcagccga tagacggtgc cgcaatacgg gcacttcgcc tcgccgacgg
     1621 cgccgacgtc gaggaacacc cgcgggtggc cgctccacag cgccatcttc gggttcgggc
     1681 agaacgcgac gccatggcct tgcaggtcct tggcgagcag ttcgacgatc tcgttgggat
     1741 tgctcatacc ttgctcaacc attcggcgta tttcgggttc cggccgttca cgatgtcgaa
     1801 gaacgccgcc tggatcttct cggtgatcgg tcctcgcgag cccgagccga gttcgatgcg
     1861 atcgagctcg cggatcggcg tgacctcggc ggcggtgccg gtgaagaacg cttcgtcggc
     1921 gatgtagacc tcgtcgcgcg tgatgcgctt ttcgaccacc ttcagcccca ggtcggcaca
     1981 gatcgcgaac acggtgttgc gggtgatgcc gttgagtgcg ccggccgaga ggtcgggcgt
     2041 gtagaccacg ccgttcttga ccacgaacag gttctcgccc gcgccttcgc tgacgaagcc
     2101 gttcgcgtcg agcagcatgg cttcgtcgta gccgtcgtcg gtcgcttcca tgttggcgag
     2161 gatcgaattc gtgtagttgc tgaccgcctt ggcctgcgtc atcgtgatgt tgacgtgatg
     2221 gcgggtgtag ctcgagatct tcacgcggat gccgcgcttc aggccttctt cgccgaggta
     2281 ggcgccccag ggccaggccg ccaccatcag gtgcactttg ttgcccttgg gcgagacgcc
     2341 gagcttctcg gagccgatcc aggtgagcgg gcgcaggtag cacgactcga gcttgttctc
     2401 gcgcaccacc gcgagctgcg cctgcaccac ctcctcgaac gagaacggga tcttcatgcg
     2461 caggatcttc gcgctgttga acagccgctc ggtgtgctcg cgcagccgga agatcgcggt
     2521 tccgtcgacg gtgttgtaag cgcgtactcc ttcgaacgcc ccacagccgt aatgcagcgt
     2581 gtgggtcagc acatggatct tggcgtcgcg ccaatcgacc atcgtgccgt ccatccagat
     2641 cttgccgtcg cgatcgtcca ttgccatggc cgcgctccat gaatgcgtgt tcgggatgcg
     2701 aattctagcg acccgggcgg agcacaccgc gcgcgccgca ccaggcgggg gcgacacgtc
     2761 gattcgtgac aaaggccgcg gaatgacgct tgcacccaac gcatcgttgg gctaggaatc
     2821 ccaaaacccc cggttcgagg gggcccgacc gcggctcacc ggtgcgaact tccattgaca
     2881 atccgacaca gccgaatcgc tcaaccagtt cgaagagcaa gacccggcga tgttcatcaa
     2941 ccgtctcctg gggagttaga tatgaggttt tccaaatcgc tgctcggcgc cgccgcggcc
     3001 ctagcgctca tcgcatcagt caccgccgct cgagctgaca ccattgacta ctcgttcaat
     3061 gtggccgcta acggcctcga tgtcagcggt ggcgactacg gcacgatcca cctcgccacc
     3121 aatgggaccg gcggcgtcga tttcaccatt acgttgcgcg gcgacctgaa tttcacgacg
     3181 accggcaaca ccggctcgca cgcactgt
//
LOCUS       DASRDG010000668         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_336108, whole genome shotgun sequence.
ACCESSION   DASRDG010000668 DASRDG010000000
VERSION     DASRDG010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336108"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /gene="ndhC"
                     /locus_tag="VFA35_11210"
     CDS             complement(<1..319)
                     /gene="ndhC"
                     /locus_tag="VFA35_11210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZT56788.1"
                     /translation="MTLDPYLPVILFILIGVAVGVAPQVLGFLLGPRRPDQAKNSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVSLREIGASGFWAMMIFLAI
                     LVVG"
     gene            complement(325..409)
                     /locus_tag="VFA35_11215"
     tRNA            complement(325..409)
                     /locus_tag="VFA35_11215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(373..375),aa:Leu,seq:gag)
     gene            complement(576..1004)
                     /gene="secG"
                     /locus_tag="VFA35_11220"
     CDS             complement(576..1004)
                     /gene="secG"
                     /locus_tag="VFA35_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZT56789.1"
                     /translation="MNLLLNLVVALQIVSALVMIGLVLIQHGKGADMGASFGSGASGS
                     LFGATGSANFMSRSTAVCAAVFFACTLTLAFYSNDRARASSGSVLDRPANAAPAASVP
                     LTGAAQVPGAASAPGAGVPAPAAPVRPAPQASGAGAVPTK"
     gene            complement(1060..1800)
                     /gene="tpiA"
                     /locus_tag="VFA35_11225"
     CDS             complement(1060..1800)
                     /gene="tpiA"
                     /locus_tag="VFA35_11225"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZT56790.1"
                     /translation="MPRKLVVGNWKMNGNRAANAELLAGLKAAGPWVADVAVCVPMPY
                     LGDVALSLQGEAIGWGAQDCSAFESGAYTGEVSAAMLAEFGCRYALVGHSERRAYHAE
                     SDQLVADKAKAALAHRITPIVCVGETLAEREAGQTDAVVKRQLSAVIHTLGHCISQIV
                     VAYEPVWAIGTGRTASPEQAQAVHALLRAQLKAATEHAVDMRILYGGSVNADNASSLF
                     AQADIDGGLIGGASLKAGDFAVICRAAG"
     gene            complement(1827..>2417)
                     /locus_tag="VFA35_11230"
     CDS             complement(1827..>2417)
                     /locus_tag="VFA35_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZT56791.1"
                     /translation="NVFDRARLKPGETLLIQGGSSGIGVTAIQLAKAAGATVIATAGS
                     DDKCAACVQLGADHAINYRQSDFVAEVKRITAGRGVDVILDMVAGGYVARELECLAED
                     GRLVFIAVQGGVQAQIDAGAVLRKRLTITGSTLRPRPIAFKAAVAAALRATAWPWIEA
                     GKVKPVIYKVFPAAQAAAAHTLMESNAHIGKLVLQW"
BASE COUNT          406 a          861 c          791 g          359 t
ORIGIN      
        1 acccgacgac gagaatcgcg aggaagatca tcatcgccca gaagccgctc gcaccgatct
       61 cgcgcaacga cacggcccac ggaaacagga aggcgatctc gaggtcgaac aggatgaaga
      121 ggatggcgac caggtagtag cgcacatcga acttcatgcg cgcgtcctcg aacgcctcga
      181 agccgcattc gtacggggag ttcttcgcct gatccggccg tctcggcccg agcagaaaac
      241 cgaggacctg cggcgccacc ccgaccgcca cgccgatcag gatgaagagg atgacgggga
      301 ggtaggggtc gagagtcatt gagttggtgc cgacggcgag actcgaactc gcacagcttt
      361 cgccactacc ccctcaagat agcgtgtcta ccaatttcac cacgacggcc aagctcggat
      421 ccgggtcgga tcttgcgctt gaaacagcac ccggatcgcg tgaggagttg ctcaccgggc
      481 agcggtggat tctaaccaca aagccccctc attttcggcg cgcgcgctgc gctgccacag
      541 caagatgacg ccccgtggcg aggcgacctg gcgcgctact tggtcggcac cgcgcccgcg
      601 ccggaggcct gcggcgctgg ccggaccggc gccgcggggg ccggcacgcc ggcccccggc
      661 gcgcttgccg cacccggcac ctgcgcggcg ccggtcaacg gcacgctcgc ggccggagcc
      721 gcgttcgccg ggcgatcgag cacgctgccg ctcgaggcgc gcgcgcgatc gttcgagtag
      781 aacgcgagcg tcagcgtgca agcgaagaaa accgccgcgc acaccgcggt cgagcgcgac
      841 atgaagttcg cgctgccggt cgcaccgaac aagctgcccg aggcgccgct gccgaacgag
      901 gcgcccatgt ccgcgccctt gccatgctgg atcagcacga ggccgatcat caccagcgcc
      961 gagacgatct gcaacgccac caccagattc aacaacaggt tcatttcttc caactccaaa
     1021 attcgtgttc gcgtgccgcg gttgcgaggc ctcgccccgt caaccggctg cgcggcagat
     1081 gacggcgaaa tcgcccgcct tcagcgaggc gccgccaatc aggccgccgt cgatgtcggc
     1141 ctgggcgaac aggctcgagg cgttgtcggc gttgacgctg ccgccgtaca ggatcctcat
     1201 gtcgaccgcg tgctcggtcg cagccttcag ctgcgcgcgc agcaacgcgt gcacggcctg
     1261 cgcctgctcg ggcgatgcgg tccggccggt gccgatcgcc cagaccggct cgtacgcgac
     1321 gacgatctgc gagatgcagt ggcccagcgt gtggatcacc gccgagagct ggcgcttgac
     1381 gaccgcatcc gtctgcccgg cttcgcgctc ggccagcgtt tcgccgacgc agacgatcgg
     1441 cgtgatgcgg tgcgcgagcg cggccttggc cttgtcggcc acgagttgat cgctctcggc
     1501 atggtaggcg cggcgctccg agtggccgac cagcgcgtag cggcaaccga actcggccag
     1561 catcgccgcc gagacttcgc cggtgtaggc gcccgactcg aaggccgagc aatcctgcgc
     1621 accccagccg atcgcctcgc cttgcagcga gagcgccaca tcgccgagat acggcatcgg
     1681 cacgcacacc gccacgtcgg ccacccacgg gccggcggcc ttcagcccgg ccaacagctc
     1741 ggcattcgct gcgcggttgc cgttcatctt ccagttgccg acgacgagtt tgcgaggcat
     1801 ggccgaagtc tccgagattg tcgccatcac cactgcagca ccagcttgcc gatgtgcgcg
     1861 ttcgactcca tcagcgtgtg ggcggcggcg gcctgtgcgg cgggaaacac cttgtagatc
     1921 accggcttga ccttgccggc ttcgatccac ggccaggcgg tcgcgcgcag cgcggccgcg
     1981 accgccgcct tgaacgcgat cggccgtggc cgcaaggtcg aaccggtgat cgtcaggcgc
     2041 ttgcgcagca cggcgcccgc gtcgatctgc gcctggaccc cgccctgtac cgcgatgaac
     2101 acgagccggc cgtcctcggc cagacactcg agctcacgcg ccacgtaccc gcccgcgacc
     2161 atgtcgagga tcacgtcgac gccgcggccg gcggtgatgc gtttcacttc tgcgacgaaa
     2221 tcggactgcc gatagttgat cgcgtgatcg gcgccgagtt gcacgcacgc ggcgcacttg
     2281 tcgtcgctgc ccgcggttgc gatcacggtc gcgccggcgg ccttggccag ttggatcgcg
     2341 gtgacgccga tgccgctcga gccgccctgg atcagcagcg tctcgccggg cttcagccgc
     2401 gcgcggtcga acacatt
//
LOCUS       DASRDG010000669         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1710397, whole genome shotgun sequence.
ACCESSION   DASRDG010000669 DASRDG010000000
VERSION     DASRDG010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710397"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFA35_11235"
     CDS             <1..435
                     /locus_tag="VFA35_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZT56792.1"
                     /translation="NVMLAPTLVKKRDTAAAAEQARQLLARVGLAEKFDAQPDQLSGG
                     QQQRVAIARALAMEPAVLLCDEITSALDPELVGEVLRVVESLADEGMTLLMVTHEMNF
                     ARKVSDRLVFMHAGRIHEIGPPEVIFGNPQTPELKQFLSSIH"
     gene            complement(445..1185)
                     /locus_tag="VFA35_11240"
     CDS             complement(445..1185)
                     /locus_tag="VFA35_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZT56793.1"
                     /translation="MSHDGPTFYDDAQVFDRYARLRERAESANDTLEAPVIKALIGAV
                     DGLDVLDLGCGDGLYGRELLAAGARSYLGIDGSMNMVRAAQASLAGTKGRVQRGDLAC
                     GVPAEPASFDLAISRLALHYVADIGPVLRGIHAALRPGGRLVFSTEHPVITCSNQALA
                     DDGLRQDWIVDDYFVPGRRVVAWLGAEVAMHHRTIEEWFAAVQGAGFVVTALRESKPQ
                     RERFADPALFERRGRIPLFLFIAARKPL"
     gene            1292..>2468
                     /gene="argG"
                     /locus_tag="VFA35_11245"
     CDS             1292..>2468
                     /gene="argG"
                     /locus_tag="VFA35_11245"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZT56794.1"
                     /translation="MATILQHLPVGQKVGIAFSGGLDTSAALHWMRNKGAIPYAYTAN
                     LGQPDEADYDEIPRKAMEYGAQAARLIDCRSQLVAEGLAALQSGAFHVSTGGLTYFNT
                     TPLGRAVTGTMLVAAMRDDDVNIWGDGSTYKGNDIERFYRYGLLANPALKIYKPWLDQ
                     LFIDELGGRAEMSAFMSAAGFKYKMSAEKAYSTDSNMLGATHEAKDLEHLSSGMKIVN
                     PIMGVAFWRDEVDVQREEVSVRFEEGVPVALNGRTFANAVELMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLFIAYERLVTGIHNEDTIEQYRMNGLKLGRLLYQG
                     RWFDPQAIMLREAAQRWVARAITGEVTLELRRGNDYSLLDTRSPNLTYQPERLTMEKG
                     "
BASE COUNT          431 a          860 c          832 g          345 t
ORIGIN      
        1 aacgtgatgc tcgcgccgac gctggtgaag aagcgcgaca ccgccgctgc agccgagcag
       61 gcaaggcagc tgctcgcgcg cgtcggcctg gccgagaagt tcgacgccca gcccgatcag
      121 ctctcgggcg ggcagcagca gcgcgtcgcg atcgcgcgcg cgctggcgat ggagccggcg
      181 gtgctgctgt gcgacgagat cacctcggcg ctcgaccccg agctcgtggg cgaggtgctg
      241 cgcgtcgtcg agtcgctcgc cgacgagggc atgaccttgc tgatggtcac gcacgagatg
      301 aacttcgcgc gcaaggtctc ggaccgcctc gtgttcatgc acgccggccg catccacgag
      361 atcggcccgc cggaagtgat cttcggcaac ccgcagacgc cggagctgaa gcagttcctg
      421 tcatcaattc attgaaccga tcgctcacag cggcttgcgc gccgcgatga acaggaacag
      481 cggaatccgg ccgcgccgct cgaacagcgc cgggtcggcg aagcgctcgc gctgcggctt
      541 cgactcgcgc agcgcggtga caacgaagcc ggcgccctgc accgcggcga accactcctc
      601 aatcgtgcgg tggtgcatcg cgacctcggc gccgagccac gcgacgacgc gccgtcccgg
      661 cacgaagtag tcgtcgacga tccagtcctg gcgcaggccg tcgtcggcga gcgcctggtt
      721 gctgcaggtg atgaccgggt gctcggtcga gaacaccagc cgcccgcccg gccgcaacgc
      781 ggcgtggatg ccgcgcagca ccggcccgat gtcggccacg tagtgcaacg cgagccgcga
      841 gatcgccagg tcgaacgacg cgggctcggc cggcacgccg cacgcaagat cgccgcgctg
      901 cacgcggccc ttggttccgg ccaggctcgc ttgggcggcg cgcaccatgt tcatcgagcc
      961 gtcgatgccg aggtaggagc gcgcgccggc ggcgagcagt tcgcgcccgt acagcccatc
     1021 gccgcagccg aggtcgagca cgtcgaggcc gtcgaccgcg ccgatcagcg ctttgatcac
     1081 cggcgcttcg agcgtgtcgt tcgcgctctc ggcgcgctcg cgcagccggg catagcggtc
     1141 gaacacctgc gcgtcgtcgt agaaggtcgg gccgtcgtgg ctcatgagtg cgtgaggctg
     1201 cggcgggcaa gcttagcgca catgccggca cgcccggccc ttgcacctaa aatcgccgat
     1261 tctcccggta gcacccactc tcctccaccc catggcaacc attcttcagc acctgcccgt
     1321 cggccagaag gtcggcatcg cgttttccgg ggggctggac accagtgccg cactgcactg
     1381 gatgcgcaac aagggcgcga tcccctacgc ctacaccgcc aacctcggcc agcccgacga
     1441 ggccgactac gacgagatcc cgcgcaaggc gatggagtac ggcgcgcagg ccgcgcgcct
     1501 gatcgactgc cgctcgcagc tcgtcgccga gggcctggcg gcgctgcaaa gtggcgcctt
     1561 ccacgtctcg accggcggcc tgacctactt caacacgacg ccgctgggcc gcgccgtcac
     1621 cggcacgatg ctggtcgcgg cgatgcgcga cgacgatgtc aacatctggg gcgacggcag
     1681 cacctacaag ggcaacgaca tcgagcgctt ctaccgctac gggctgctcg ccaacccggc
     1741 gctgaagatc tacaagccct ggctcgacca gctgttcatc gacgagctcg gcgggcgcgc
     1801 cgagatgtcg gcattcatga gcgcggccgg cttcaagtac aagatgtcgg ccgagaaggc
     1861 ctactcgacc gactcgaaca tgctcggtgc gacacacgag gcgaaagacc tcgagcacct
     1921 gagcagcggc atgaagatcg tcaatccgat catgggcgtg gcattctggc gcgacgaggt
     1981 ggacgtccaa cgcgaagagg tcagcgtgcg cttcgaggaa ggcgtgccgg tcgcgctgaa
     2041 cggccggacg tttgcgaacg cggtcgagct gatgctcgag gcgaatcgca tcggcgggcg
     2101 ccacggcctg ggcatgagcg accagatcga gaaccgcatc atcgaggcca agagccgcgg
     2161 catctacgaa gcgccgggcc tggcgctgct gttcatcgcc tacgagcggc tcgtcaccgg
     2221 catccacaac gaggacacga tcgagcaata ccgcatgaac ggcctgaagc tcggccgact
     2281 gctgtaccag ggccgctggt tcgacccgca ggcgatcatg ctgcgcgagg cggcgcagcg
     2341 ctgggtcgcg cgcgcgatca cgggtgaggt aacgctggag ctgcgccgcg gcaacgacta
     2401 ctcgctgctc gacacccgct cgcccaacct cacctaccag cccgagcggc tgacgatgga
     2461 aaagggcg
//
LOCUS       DASRDG010000670         7375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1137927, whole genome shotgun sequence.
ACCESSION   DASRDG010000670 DASRDG010000000
VERSION     DASRDG010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137927"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="VFA35_11250"
     CDS             <1..1583
                     /locus_tag="VFA35_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZT56795.1"
                     /translation="AKLIVIDPRRIDLVKTPHGKATYHLKLRPGTNVAMLNALAHVIV
                     KEGLLDQAFIDQRCDGDAFRQWCDFVAAEENSPETLETDTGVPAELVREAARLYATGG
                     NAAIYYGLGVTEHTQGSTAVMAIANLAMATGNIGRPGVGVNPLRGQNNVQGSCDIGSF
                     PHELPGYRHVSDTTVRQAFEAAWGVPLHSEPGLRIPNMFDAAVDGSFMGLYCEGEDIV
                     QSDPDTQHVSHALEAMECIVVQDLFLNETAKYAHVFLPGTSFLEKDGTFTNAERRISR
                     VTKVMPPQPGYADWEVTMMLSNALGYPMNYTHPREIMAEIAALTPTFSGVSYELIDKL
                     GSVQWPCNAQAPNGTPTMHIDAFVRGKGKFIITQYVASDEKVTRKFPLLLTTGRILSQ
                     YNVGAQTRRTENNQWHSEDRLEIHPVDAEDRGIRDGDWVGIQSRAGDTVLRAVVSERM
                     QPGVVYTTFHFPESGANVITTDNSDWATNCPEYKVTAVQVMPVSQPSEWQREYSRFDK
                     AQAELLQQRHAAEAVTGK"
     gene            1601..1825
                     /locus_tag="VFA35_11255"
     CDS             1601..1825
                     /locus_tag="VFA35_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HZT56796.1"
                     /translation="MNNDNLIRMVNRIGTFFAAMPDREEAMSDIAQHVRRFWEPRMRK
                     QLFEHLDTKHGEGVDAIVVDALARHRALFS"
     gene            1961..2644
                     /locus_tag="VFA35_11260"
     CDS             1961..2644
                     /locus_tag="VFA35_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZT56797.1"
                     /translation="MQYPERSQDINLKNPKTPSNLSGEIFGTLVNLSGRRRFTSQRVV
                     LFAVLAAQGRDRARQMADDALTAFRDAHSALVHGTDRVPGIFCDELQEAYFGASQGDK
                     VIRDFIGLAGRALDAIEACSPGAPSLLEQLVESATPLLAVLNGITQVYDDLAKRQASL
                     AKKQLTGIMSDIESIAKQAHIVGFNAQIVAARAGASGREFSVVASELSRITGKIDELV
                     REALRASAA"
     gene            complement(2669..4087)
                     /gene="pabB"
                     /locus_tag="VFA35_11265"
     CDS             complement(2669..4087)
                     /gene="pabB"
                     /locus_tag="VFA35_11265"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HZT56798.1"
                     /translation="MAEIEVTIRRVTLPDGPRGTMARLRGEGRYALLESALPMPRQAE
                     WSYIAGPALATLYTDETSTRLEREGDVLACWDDPFEALAAVGGAHAGGVRFDGECPQG
                     MDFVGGWVGVLGYDLARHIEPLPEQARHDPVLPRQWWMAADQVLAFHHPSGQWWHATL
                     RDPAACLPWSRPGREPVWKCSLAQARGPAPARGQWRAGARSLRTSRAQFESGVRTIRA
                     AIARGDVLQVNLTRREEAAFTGDAWALYEDLAVAHPAPFAAYVEGPGFAIASCSPERF
                     LRRHGDLVEARPIKGTAARGGDEAEDLAQREWLAASIKDRAENLMIVDLMRNDLGRVA
                     VTGSVRVPELFALEPYASVWQMVSTVRARLRPDCGVAQLLRACWPPGSMTGAPKIKAM
                     QIIEALEPLRRGCYAGSVGYLDCRGNMDLSVVIRTAIVAGARVMLQLGGGIVADSEPA
                     AEWAETVAKGRRIVGTLDRGGG"
     gene            4086..4664
                     /locus_tag="VFA35_11270"
     CDS             4086..4664
                     /locus_tag="VFA35_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56799.1"
                     /translation="MSSLARQTPQRQRFHDPSDAPALAAHARGMLKLLLAQDGSTTRL
                     CETIAAGPVALQVLAQRVVHAAPGEVHDTLPGASFIERITCLAAHGEVMMDNLSYIAL
                     DGLAPDLRRDLEAGAMPIGHLLARLWVRRAFLEAAPMMYERLWGVVGLPDPGASRAYR
                     IVTPEGARMLIAETYRRGMLMACDGARRVATG"
     gene            complement(4607..5875)
                     /locus_tag="VFA35_11275"
     CDS             complement(4607..5875)
                     /locus_tag="VFA35_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZT56800.1"
                     /translation="MSSAAIVDAAFLADPYPTYTALREAGPIHWSEAFFGGAWLLTRH
                     ADVEAVLRDSARFSAQRTGGWVMQDAAARGELKRFQQLFARAMLFVDAPDHTRIRGVL
                     NAGFRGELLQRLVPAIEQRVDALLDELSAVDVEAGFDFMQALARPLPADVMALLMDIP
                     AADRGDFIAWSDDLAAFIGATLPSLEQARRAQTSLLAMCGYFERLVPLRRAAPGDDLV
                     SRLLRAEAAGEIRGAAELLAQCAMLLFAGHETTRNLLGNGLQALLAQPDQWQRLQREP
                     GLLPGAVRELLRFESPVQYTGRRIATDLVLHGQQLRRGDLVVALIASANRDPARYEHP
                     ERLDIARRPGGLLSFGAGPHVCIGAGLTMMEAEIVFGRLLRRFPRLCLAAAQPRWSGN
                     AVYRGLDALPVRQPVATRLAPSHAISMPRR"
     gene            complement(5872..6555)
                     /gene="bioD"
                     /locus_tag="VFA35_11280"
     CDS             complement(5872..6555)
                     /gene="bioD"
                     /locus_tag="VFA35_11280"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HZT56801.1"
                     /translation="MTGTDTEVGKTRVSAALVHWMARRGRVAAGFKPVAAGTVLIGGT
                     RVNEDVRRLREASSAAVTDAEVGPFQFDAACAPHLAAALEGRAIDPGRILAAGRALLA
                     RCEFVVVEGVGGFCVPLGPDWDSSHIACGLALPVVLVVGMRLGCLNHALLTAEAIKAR
                     RLPLAGWIANTVDPSMAYLDRNLSTLRHELDERRQVPCLGVVPRLDDPCPDAVAAHLN
                     DAALRSVFA"
     gene            complement(6585..>7375)
                     /locus_tag="VFA35_11285"
     CDS             complement(6585..>7375)
                     /locus_tag="VFA35_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZT56802.1"
                     /translation="LIDGALLAKAPLQRYAHGRTDVLADQLARCGTPIKLIVTDAVFS
                     MDGDLAPLPELLALAERFDAWIIVDDAHGFGVLGEQGRGALSHFGLRSERFICMGTLG
                     KAAGIAGAFVAADATIVDWLVQAARSYIYTTAAPPALSHALRASLELIRGEEGEQRRA
                     RLAALIGLLRRRLGALIAATPRLGWTLAESATAIQPVIVGDNEAALALSAALESRGLW
                     VPAIRPPTVPLGSARLRITLSAAHTEDDVLRLVDALEHAARALA"
BASE COUNT         1188 a         2639 c         2540 g         1008 t
ORIGIN      
        1 gcgcgaagct gatcgtgatc gatccgcgtc gcatcgacct ggtcaagacg ccgcacggca
       61 aggccacgta tcacctcaag ctgcgtcccg gcaccaacgt cgcgatgctc aatgcgctcg
      121 cacacgtcat cgtcaaggaa gggctgctgg atcaggcttt catcgaccaa cgctgcgatg
      181 gcgatgcctt ccggcaatgg tgcgatttcg tcgctgcgga agagaactcc ccggaaacgc
      241 tcgagaccga caccggcgtg cctgcggagc tggtccgcga agccgctcgc ctctacgcca
      301 ccggcggcaa cgccgcgatc tattacggac tcggcgtcac cgaacacacg caaggatcga
      361 ctgcggtgat ggcgatcgcc aacctggcaa tggccaccgg caacatcggg cgtcccggcg
      421 tcggcgtgaa tccgctgcgc ggccagaaca acgtgcaggg ctcgtgcgac atcggctcgt
      481 tcccgcacga gttgccgggc taccggcacg tctccgacac gaccgtgcgc caggcgttcg
      541 aggccgcctg gggcgtgccg ctgcacagcg aacccggcct gcgcatcccg aacatgttcg
      601 acgcggcggt cgacggcagc ttcatgggcc tgtactgcga aggcgaggac atcgtgcagt
      661 ccgacccgga cacccagcac gtgtcgcatg cgctcgaggc gatggagtgc atcgtcgtgc
      721 aggatctgtt cctgaacgag accgcgaagt acgcgcacgt gttcctgccg ggcacctcgt
      781 tcctcgagaa ggacggcacg ttcacgaacg ccgagcggcg catctcgcgc gtcaccaagg
      841 tgatgccgcc ccagcccggc tacgccgact gggaagtgac gatgatgctg tcgaacgcgc
      901 tcggctaccc gatgaactac acgcacccgc gcgagatcat ggccgagatc gcggcgttga
      961 cgccgacctt ctcgggcgtc agctacgagc tgatcgacaa gctcggcagc gtgcagtggc
     1021 cgtgcaacgc gcaggccccg aacggcacgc cgacgatgca catcgacgcg ttcgtgcgcg
     1081 gcaagggcaa gttcatcatc acgcagtacg tcgccagcga cgagaaggtg acgcgcaagt
     1141 tcccgctgct gctgaccacc gggcgcatcc tgtcgcagta caacgtcggc gcgcagacgc
     1201 gccgcaccga gaacaaccag tggcacagcg aggaccggct cgagatccac ccggtcgatg
     1261 cggaagaccg cggcatccgc gacggcgact gggtcggcat ccaaagccgc gccggcgaca
     1321 cggtgctgcg cgcggtcgtc agcgagcgca tgcagccggg cgtcgtctac acgaccttcc
     1381 acttccccga atcgggcgcg aatgtgatca cgaccgacaa ctccgactgg gccaccaact
     1441 gccccgagta caaggtcacg gcagtgcagg tgatgccggt gtcgcagccc tccgagtggc
     1501 agcgcgagta cagccgcttc gacaaggcgc aggccgagtt gctgcagcag cgccacgcgg
     1561 ccgaagcggt gacgggcaaa tgacgcgagc cgactgagcc atgaacaacg acaacctgat
     1621 ccgcatggtc aaccgcatcg gcaccttctt cgccgcgatg cccgatcgtg aagaagccat
     1681 gagcgacatc gcgcagcacg tgcggcgctt ctgggaaccg cgcatgcgca agcaattgtt
     1741 cgagcacctc gacacgaagc acggcgaagg cgtcgatgcg atcgtcgtcg atgcgctcgc
     1801 gcgccaccgg gcgttgttca gctgagggtc cggggggcgc ggcggctggg catcgcgcct
     1861 ggatgtcgct ggtacggacc gtgctacagc gtggctcagc gccgcgagtc gcaccgcttc
     1921 ggcgttcttg ggctcgcgca tgtcgatgtg gagccctcgg atgcagtacc cggaacgtag
     1981 ccaagatatc aatctcaaga acccgaagac accttcgaac ttgtccggcg agatcttcgg
     2041 cacgctggtc aacctctcgg gccgaaggcg cttcacgtcg cagcgcgtcg tgttgtttgc
     2101 agtgctggcg gcgcaggggc gcgaccgggc acgccagatg gccgacgatg cgctgacggc
     2161 gtttcgagac gcgcacagcg ccctggtcca cggaaccgat cgcgtgcccg gcatcttctg
     2221 cgacgagttg caggaggcgt acttcggggc gtcgcaaggc gacaaggtca ttcgcgattt
     2281 catcgggctt gccgggcgcg cgctcgatgc catcgaggcg tgctcgcccg gcgcaccgtc
     2341 gctgctggag cagctcgtgg agagcgcgac gccactgctg gccgtgctca acgggatcac
     2401 ccaggtctac gatgacctgg cgaagcgcca ggcctctctc gcgaagaagc agttgaccgg
     2461 gatcatgagc gacatcgaat cgatcgccaa gcaggcgcac atcgttgggt tcaacgccca
     2521 gatcgtcgcg gctcgcgccg gcgcgtcagg ccgggaattc tcggtcgtgg caagcgaatt
     2581 gtctcggatc acgggcaaga tcgacgaact ggtgcgcgaa gcgctgcggg cctcggccgc
     2641 ctgaggcctc gatgcggcgc cgcatcggtc atccgccgcc ccgatccagg gtgccgacga
     2701 tgcgccgccc cttggccacc gtctcggccc actcggccgc gggctcggag tccgcgacga
     2761 tgccgccgcc gagctgcagc atcacgcgag cgccggccac gatggccgtg cgaatcacga
     2821 ccgacaggtc catgttgccg cggcagtcca ggtagcccac cgagccggcg tagcagccgc
     2881 ggcgcagggg ttcgagcgcc tcgatgatct gcatcgcctt gatcttcggc gcgccggtca
     2941 tcgagcccgg cggccagcag gcgcgcagca gctgcgcgac gccgcagtcg ggacgcagcc
     3001 gcgcgcgcac ggtcgagacc atctgccaca cgctggcata gggctcgagc gcgaacagct
     3061 cgggcacccg cacgctgccg gtcacggcca cgcgaccgag gtcgttgcgc atcaggtcga
     3121 cgatcatcag gttctcggcg cgatccttga tgctcgcggc cagccactcg cgttgcgcga
     3181 ggtcttcggc ttcgtcgccg ccgcgggcgg cggtcccctt gatgggacgc gcttcgacga
     3241 gatcgccgtg gcgccgcagg aagcgttcgg gcgagcagct ggcgatcgcg aacccgggcc
     3301 cttcgacgta ggccgcgaac ggcgccggat gcgccaccgc gaggtcttcg tacaaggccc
     3361 aggcgtcacc cgtgaacgcg gcctcttcgc ggcgcgtcag gttgacctgc agcacgtcgc
     3421 cgcgggcgat ggccgcacgg atggtccgca cgccggactc gaattgcgcg cgcgacgtgc
     3481 gcaggctgcg cgcgccggca cgccattgac cacgcgcggg cgcggggccg cgggcctgcg
     3541 ccaagctgca cttccagacc ggttcgcgac cggggcgcga ccacggcaag cacgcggccg
     3601 ggtcacggag ggtcgcgtgc caccattgcc cgctcggatg atgaaacgcg agcacctgat
     3661 ccgccgccat ccaccattgc ctgggcagca ccgggtcgtg ccgcgcctgc tcgggaagcg
     3721 gctcgatgtg gcgcgccagg tcgtagccca gaacccccac ccagccgccg acgaagtcca
     3781 tgccctgcgg acactcgcca tcgaaacgaa caccccccgc gtgagcgccg ccgaccgccg
     3841 cgagcgcttc gaagggatcg tcccagcacg cgagcacgtc gccctcgcgt tcaagccggg
     3901 tcgaggtttc gtctgtgtac agtgtggcca gcgccggccc ggcgatgtag gaccattcgg
     3961 cctggcgcgg catcggcagc gcgctttcca gcagcgcata gcggccctcg ccgcgcagcc
     4021 gcgccatcgt gccgcgcggt ccatcgggca gggtcacgcg gcgaatcgtc acttcaatct
     4081 cagccatgtc ttcactcgct cgccagacgc cgcagcgtca acgcttccac gacccgtcgg
     4141 acgcgccggc gctggcggcg cacgcgcgcg gcatgctgaa actgctgctg gcgcaggacg
     4201 gttcgacgac ccgattgtgc gaaacgatcg cggccggccc ggtcgcgctg caggtgctgg
     4261 cgcagcgggt cgtgcacgcg gcgcccggcg aagtccacga cacgctgccc ggcgcgagct
     4321 tcatcgagcg catcacctgc ctggccgcgc acggcgaggt gatgatggac aacctgtcgt
     4381 acatcgcgct cgacggcctg gcgccggacc tgcgccgcga cctcgaggcc ggagccatgc
     4441 cgatcggtca tctgctggcg cggctgtggg tgcggcgcgc gtttctcgaa gcggcgccga
     4501 tgatgtacga gcggctctgg ggtgtcgtcg ggctgcccga ccccggcgcc tcgcgcgcgt
     4561 accgcatcgt cacgcccgag ggggctcgca tgctgatcgc cgagacctac cggcgcggca
     4621 tgctgatggc gtgcgacggc gccaggcgcg tggcgacggg ttgacgcacc gggagcgcat
     4681 ccagaccgcg gtacaccgca ttgccgctcc agcgcggctg cgcagcggcc aggcacagtc
     4741 gcgggaatcg acgcaacagg cggccgaaga cgatttcggc ctccatcatg gtcaggccgg
     4801 cgccgatgca cacatgcggt ccggcgccga aggacagcag gccgccgggg cgacgcgcga
     4861 tgtccagtcg ctcgggatgc tcgtagcgcg ccgggtcgcg gttggcgctc gcgatcagcg
     4921 cgacgaccag gtcgccgcgg cgcaactgct ggccgtgcag caccaggtcg gtcgcgatgc
     4981 ggcggccggt gtactgcacc gggctctcga agcgcagcag ctcgcgcacc gcgcccggca
     5041 acaggccggg ctcgcgctgc aggcgctgcc actggtccgg ctgcgcgagc agcgcctgca
     5101 ggccgttgcc cagcaggttg cgcgtggtct cgtggccggc gaacagcagc atcgcgcact
     5161 gggccagcag ctcggcagcg ccgcggatct cgccggccgc ctcggcgcgc agcagccggc
     5221 tcaccaggtc gtcgcccggc gcggcgcggc gcagcggcac gagccgctcg aagtagccgc
     5281 acatcgccag caggctggtc tgcgcgcggc gggcctgctc gagcgatggc agcgtggcgc
     5341 cgatgaacgc cgccaggtcg tcggaccagg cgatgaagtc gccgcggtcg gccgccggga
     5401 tgtccatcag cagcgccatc acgtcggccg gcagcggccg cgccagtgcc tgcatgaagt
     5461 cgaagccggc ctcgacgtcc accgcactca actcgtcgag caaggcatcg acgcgctgct
     5521 cgatggccgg cacgagccgc tgcagcagct cgccgcgaaa ccccgcattc agcacaccgc
     5581 gaatgcgcgt gtgatccggt gcatcgacga acagcatcgc gcgcgcgaac agctgctgga
     5641 agcgcttcag ctcgccgcgc gccgcagcgt cttgcatcac ccagccgccg gtgcgctgcg
     5701 ccgagaagcg cgccgaatcg cgcagcaccg cctcgacatc ggcatgccgg gtcagcagcc
     5761 aggccccgcc gaagaacgcc tcgctccagt ggatcgggcc ggcctcgcgc aacgcggtgt
     5821 aggtcggata cgggtcggcc aggaacgcgg cgtcgacgat cgcggccgag ctcatgcgaa
     5881 caccgaccgc agtgcggcat cgttcagatg cgcggccacg gcgtccgggc acgggtcatc
     5941 gagccgcgga acgacgccga ggcagggcac ctggcggcgc tcgtccagtt cgtgccgcag
     6001 cgtggacagg ttgcggtcca gatacgccat gcttgggtcg acggtgttcg cgatccagcc
     6061 ggccagtggc aggcggcgcg ccttgatcgc ctcggcggtc agcagcgcgt gattcaggca
     6121 gccgagccgc atgccgacca ccaggacgac cggcagcgcg aggccgcagg cgatgtggct
     6181 gctgtcccag tcggggccca gcggcacgca aaagccgccc acgccttcga ccaccacgaa
     6241 ctcgcagcgc gccagcagcg cccggcctgc cgccaggatc cgacccggat cgatcgcccg
     6301 gccctccagc gcggcggcga gatgcggggc acaggcggcg tcgaactgga acggcccgac
     6361 ctccgcatcg gtgacggcgg ccgaactcgc ctcgcgcagt cggcgcacgt cctcgttgac
     6421 ccgcgttccg ccgatcagca ccgtgccggc ggccaccggc ttgaagccgg cagcgacccg
     6481 gccgcgccgc gccatccagt gaaccagtgc ggcgctgacg cgcgtcttgc cgacctccgt
     6541 gtcggtgccg gtcacgaagc agcccggcgg cccggtgcgc gcgttcatgc cagcgccctc
     6601 gcggcatgtt cgagcgcatc gaccagccgc agcacatcgt cctcggtgtg cgccgcgctc
     6661 agcgtgatgc gcaagcgcgc cgaaccgagc gggaccgtcg gcgggcggat cgccggcacc
     6721 cacagcccgc gcgattcgag cgccgccgac agcgccagtg cggcctcgtt gtcgcccacg
     6781 atcaccggct ggatcgccgt ggccgactcg gccagtgtcc agccgagccg tggcgttgcc
     6841 gcgatcaacg cgccgagccg tcggcgcagc agcccgatca gcgcggcgag ccgagcgcgc
     6901 cgttgctcgc cttcttcgcc ccggatcaac tccaggctcg cgcgcagcgc atgcgacagc
     6961 gcgggcggcg cggcggtcgt gtagatgtag ctgcgcgccg cctggaccag ccagtcgacg
     7021 atcgttgcat cggccgcgac gaaggcgccg gcgatgccgg cggccttgcc gagcgtgccc
     7081 atgcagatga agcgctcgct gcgcagcccg aaatgcgaca gcgcgccccg gccctgctcg
     7141 cccagcaccc cgaagccgtg ggcgtcgtcg acgatgatcc aggcatcgaa gcgctcggcc
     7201 agcgccagca gctcgggcag cggcgcgaga tcgccgtcca tgctgaacac cgcgtcggtc
     7261 acgatcagct tgatcggcgt gccgcagcgc gcgagctgat ccgccagcac gtccgtccgc
     7321 ccatgcgcgt agcgctgcag gggcgctttg gccagcaagg cgccgtcgat cagcg
//
LOCUS       DASRDG010000671         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1257557, whole genome shotgun sequence.
ACCESSION   DASRDG010000671 DASRDG010000000
VERSION     DASRDG010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257557"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFA35_11290"
     CDS             <1..665
                     /locus_tag="VFA35_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZT56803.1"
                     /translation="INYYDGQGFMVPKKLNVDSAKKLNGATLCVQAGTTSEKNVADYF
                     GANAMKYKPVVFDTAEAITSAFFAGRCQVYTTDMSDLAGARTKAPNADDYVILPQVIS
                     KEPLGPSVRRGDDEWFQIVRWTLFAMIEAEEEGLTQANVDEQKTSNKDPGVQRFLGVS
                     EDTGKLIGLDAAWAHRIVKQVGNYGESFERNLGPKTPVALPRGVNNLWTKGGLMYAPP
                     LR"
     gene            667..1818
                     /locus_tag="VFA35_11295"
     CDS             667..1818
                     /locus_tag="VFA35_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429249.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZT56804.1"
                     /translation="MIGADAAWRARRRARLRRDWLLQGAVALVLVALALLLARNVGAN
                     LEARQIKSGFDFLSDPAGFNIGELLFDYTSRDSYLRAFAIGMSNTLRVALAGVVLASV
                     LGVGIGLLRLSHHPLVSGLCAAYVEIFRNIPLLIQLLAIYLLITELLPGSNDALSVDG
                     VALLSKEGLQIAVPESGRLALLCALVAFAVGAMLGMHAAHRWLEVGVSLVALASGAVA
                     ASGAWLIVGATGGWSRPVLDGFLISGGASLTPEFLALWLGLALFTSASIAEIVRAGAL
                     AVPAGQWDAALALGMTRPQAIAGAIFPQALRLAVPPLASQYMNLIKNSSLAVVVGYPD
                     LVSIGNTSINQNGQALEVIVIIMAAYLSLNLVVAAVMNAVNARVTRAPR"
     gene            1833..>2514
                     /locus_tag="VFA35_11300"
     CDS             1833..>2514
                     /locus_tag="VFA35_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434085.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZT56805.1"
                     /translation="MAAPDRIAPPPARLFAQARTRLFAGPGASLVTLVLLGALAWAGW
                     RLLAWGVIDAVAAPDYAACKAAPGACWGFVAEKWRLIVFGRYPYEQQWRPALATGAVL
                     AMLVASALPALWSRHGVRMLALGWLLAFALFFTLMFGGVFGLEPVGTDRWGGLPLTVI
                     LTLFGMVASTPIGIALALARRSSLALLRSLAIGYIELVRGVPLITVLFVATFVFPLVL
                     PAGWRLDPF"
BASE COUNT          332 a          898 c          854 g          430 t
ORIGIN      
        1 cgatcaacta ctacgacggc cagggcttca tggtgcccaa gaagctgaac gtcgacagcg
       61 cgaagaagct caacggcgcg actttgtgcg tgcaggccgg caccaccagc gagaagaacg
      121 tcgccgacta cttcggcgcg aacgcgatga agtacaagcc ggtcgtgttc gacaccgccg
      181 aagcgatcac gagcgcgttc ttcgccggcc gctgccaggt ctacacgacc gacatgagcg
      241 acctcgccgg agcgcgcacc aaagcgccca atgccgacga ctacgtgatc ctgccgcagg
      301 tgatctcgaa ggagccgctc ggcccgtcgg tgcgacgcgg cgacgacgaa tggttccaga
      361 tcgtccgctg gacgctgttc gcgatgatcg aagccgagga agagggtttg acgcaggcga
      421 acgtcgacga gcagaagacg agcaacaagg atccgggcgt gcagcgcttc ctcggcgtct
      481 ccgaggacac cggcaagctg atcggcctcg acgcggcgtg ggcccaccgc atcgtcaagc
      541 aggtcggcaa ctacggcgaa agcttcgagc gcaacctcgg gccgaagacg ccggtggcgc
      601 tgccgcgcgg cgtcaacaac ctgtggacca agggcggcct gatgtacgcc ccgccgctgc
      661 gctgaagtga tcggcgccga cgccgcctgg cgcgcccgcc ggcgagcgcg gctgcggcgc
      721 gactggctgc tgcagggcgc cgtcgccctg gttctggtcg cgctcgcgct cctcctcgcg
      781 cgcaacgtcg gagccaacct cgaagcgcgg cagatcaagt cgggcttcga cttcctgagc
      841 gacccggccg ggttcaacat cggcgagctg ctgttcgact acacgtcgcg cgacagctac
      901 ctgcgcgcgt tcgcgatcgg catgagcaac acgctgcgcg tcgcgcttgc cggcgtggtg
      961 ctcgcgagcg tgctcggcgt cggcatcggc ctgctgcgcc tctcgcacca cccgctcgtt
     1021 tccgggctct gcgccgccta tgtcgagatc ttccgcaaca tcccgctgct gatccagttg
     1081 ctcgcgatct acctgctgat caccgagctg ctgccggggt cgaacgacgc gttgtcggtc
     1141 gacggcgtcg ccctcctttc gaaggaaggg ctgcagatcg cggtgccgga gtccggcagg
     1201 ctcgcgctgc tgtgcgcgct cgtcgccttc gccgtcgggg cgatgctggg catgcacgca
     1261 gcgcaccgct ggctcgaagt cggcgtcagc ctcgtcgcgc tcgcgagcgg cgccgtggcg
     1321 gcgagcggcg catggctgat cgtcggcgcc accggaggat ggtcgcgacc ggtccttgac
     1381 ggcttcctga tcagcggcgg cgcgtccctc actcccgagt tcctcgcgtt gtggctcggc
     1441 ctggccctct tcacttcggc gtcgattgcc gagatcgtcc gcgccggagc gctcgccgtg
     1501 cctgccggcc agtgggacgc cgccctcgcg ctcggcatga cgcgcccgca ggcgatcgcc
     1561 ggtgcgatct ttccgcaggc gctgcgcctc gcggttccgc cgctcgcgag ccagtacatg
     1621 aacctcatca agaactcgtc gctggcggtc gtggtcggct atccggatct ggtgtcgatc
     1681 ggcaacacat cgatcaacca gaacggccag gcactcgagg tcatcgtcat catcatggcg
     1741 gcgtacctga gcctgaacct cgtcgtcgcg gcggtcatga atgcggtcaa tgcgcgcgtc
     1801 acgcgcgcgc cgcgctagcg cggtcggcat cgatggctgc gcccgaccgg atcgctcctc
     1861 cgccggcgcg cctgttcgcc caggcgcgca cgcgcctctt cgccgggccc ggcgcgagcc
     1921 tggtcacgct cgtcctgctc ggtgcgctcg cctgggccgg ctggcgcctg ctcgcctggg
     1981 gcgtgatcga cgccgtcgcc gcgccggact acgcggcctg caaggcggcg ccgggagcgt
     2041 gctggggctt cgtcgccgag aagtggcgcc tgatcgtgtt cggtcgctac ccctacgagc
     2101 agcagtggcg gcctgcgctg gcgaccggcg cggtgctggc gatgctggtc gcgagcgccc
     2161 tgccggcgct ctggtcgcgg cacggcgtgc gcatgctcgc cctcggctgg ctgcttgcct
     2221 tcgccctctt cttcaccctg atgttcggcg gcgtgttcgg cctcgagccc gtaggcaccg
     2281 accgctgggg cggccttccg ctcaccgtga tcctgacgct gttcggcatg gtcgcatcga
     2341 cgccgatcgg catcgcgctc gcgctcgcgc ggcgctcgtc gttggcgctg ctgcgctcgc
     2401 tcgccatcgg ctacatcgag ctcgtgcgcg gcgtgccgct gatcaccgtg ctgttcgtcg
     2461 cgaccttcgt gttcccgctc gtccttccgg ccggctggcg gctcgatccg ttct
//
LOCUS       DASRDG010000672         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1710594, whole genome shotgun sequence.
ACCESSION   DASRDG010000672 DASRDG010000000
VERSION     DASRDG010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710594"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /gene="tssK"
                     /locus_tag="VFA35_11305"
     CDS             complement(<1..1066)
                     /gene="tssK"
                     /locus_tag="VFA35_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HZT56806.1"
                     /translation="MTWHNKVVWTEGMFLQPQHFQQHDRFTEYQLRQRLAATLGHAWG
                     FTSVAVDDAALALGKLSINSAQGVLPDGTAFSIPSNDAAPAAIDIPADARNERVVLAL
                     VLQRPGIVESDAEQAAGAMGPRYTVAEVEVGDSNTNVDRDTAIQIGRLNLRLMLERDA
                     GEGYATIGVARVVERRADNKLVLDTQYIPPMMHAQGHQILDGYVREICGLLHQRGEAL
                     AARLSQPGRGGVAEIADFLLLEAVNRSQPVFMQLQQRSVLHPEILYAACLSLAGDLST
                     FREKRRPPPFPEYLHDDLQKCFRAVMDDLRQSLSMVMEQTAIPIELQDRKHGIRVSVI
                     PDIDLQRNAQFVLAVNAQMPG"
     gene            1459..1956
                     /locus_tag="VFA35_11310"
     CDS             1459..1956
                     /locus_tag="VFA35_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZT56807.1"
                     /translation="MLAALFACGPAFAQSSLDIGTSVPDAASVKDGLFPEDACKELEA
                     SGFKCMGLKPAVKYSLPASSFKVGSAELPELLKKQLDVFAEVLRSKKGTARQIKVTGH
                     ADASGTPEANQQLSIRRAEAVKEYLVQKGADPNMLTIYGQGSKDPVNPADPLAPENRR
                     VEIGR"
     gene            complement(2027..2416)
                     /locus_tag="VFA35_11315"
     CDS             complement(2027..2416)
                     /locus_tag="VFA35_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HZT56808.1"
                     /translation="MANHVCMGALLQCSFGVAPSSLVVLPVNKVLTSFVPAANIMDNK
                     PMVNIMPFGMCSSPSNPVVAAATAAALGVLTPMPCIPNTTTPWAPGAPNVMLANQPAL
                     DDTSILNCMWGGVIKVNFAGQVTEQIP"
     gene            complement(2433..3182)
                     /locus_tag="VFA35_11320"
     CDS             complement(2433..3182)
                     /locus_tag="VFA35_11320"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZT56809.1"
                     /translation="MELATLSRRGGRNYNEDACGHWHSDSHLCCVVADGAGGHGGGDK
                     ASKLAVQFILGAFAGAPDGTPEAIERMIVATNRSIIQHRADDRALQNMHSTVVALFID
                     FEARAGTWGHCGDSRLYVFRAGKVAARTRDHSLVQSLVDGGMLAAEDMRSHPQRSELL
                     SALGVGEEDLQVSVSAPPWQVEAGDAFLLCTDGMWEYVEDAQMEAALTQAVSPSAWLK
                     VLEQAVLHGASHKPRHDNFTALAVWVSEPGP"
     gene            complement(3226..>3661)
                     /gene="tagH"
                     /locus_tag="VFA35_11325"
     CDS             complement(3226..>3661)
                     /gene="tagH"
                     /locus_tag="VFA35_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HZT56810.1"
                     /translation="EIRAEVTMIVTRENNPLKFSPSVEVALGHLLSPPARGFMESDKA
                     MRDAYDDLRAHQFGFLAGMRAALEGILKRFDPATLEGKLTQKSLLSSILPGQSKAQMW
                     EVFTQLYSQISSEASDDFHELFGKEFLRAYNEYIDQLAKDRP"
BASE COUNT          585 a         1207 c         1258 g          611 t
ORIGIN      
        1 cgcccggcat ctgcgcattg accgcgagca cgaactgcgc gttgcgctgc aggtcgatgt
       61 cggggatcac ggagacgcgg atgccgtgct tgcggtcctg cagctcgatc gggatggcgg
      121 tctgctccat caccatcgac agcgactggc gcaggtcgtc catcacggcg cggaagcact
      181 tctgcagatc gtcgtgcagg tattcgggga acggcggcgg acggcgcttc tcgcggaagg
      241 tggacaggtc gccggccagg ctcaggcagg cggcatagag gatttccggg tgcagcaccg
      301 agcgctgctg caactgcatg aacaccggct gggaacggtt caccgcctcc agcagcagga
      361 agtcggcgat ctcggcgacg ccgccgcggc cgggctgcga caggcgcgct gccagggctt
      421 cgccgcgctg gtgcagcagg ccgcagatct cgcgcacgta gccgtcgagg atctgatggc
      481 cctgcgcatg catcatcggc gggatgtact gggtgtcgag caccagcttg ttgtcggccc
      541 ggcgctcgac gacgcgggcc acgccgatcg tcgcatagcc ttcgccggcg tcgcgctcga
      601 gcatcaggcg caggttcagc cggccgatct ggatcgcggt gtcgcggtcg acgttggtgt
      661 tgctgtcgcc gacctcgacc tcggccacgg tgtagcgcgg ccccatcgcc ccggcggcct
      721 gctcggcgtc cgactcgacg atgccggggc gctgcaggac gagggcgaga acgacgcgct
      781 cgttgcgcgc gtcggccggg atgtcgatcg ccgccggcgc ggcatcgttg ctgggaatcg
      841 agaacgccgt gccgtcgggc aggacgcctt gcgccgagtt gatgctcagc ttgccgaggg
      901 cgagcgcggc gtcgtcgaca gccacggaag tgaagcccca cgcatgaccg agggttgcgg
      961 caagacgctg gcgcagttgg tactcggtga agcgatcatg ctgctggaag tgctggggct
     1021 gcaggaacat cccctccgtc cagaccacct tgttgtgcca ggtcatagcg ataagtgaac
     1081 tgtggcttcc cttgcgactg cacgtcggca aaagccgcgg ccgttctctt gttcgtgcag
     1141 caccgatgcg ccgtatcgcc gatcgagcag gcggatggcg gcctcgcact ttacttgaat
     1201 gcgacgccgc tcgaaacaga atctccgcgc ggcgagcaca aggccacgaa ccgcggcacc
     1261 gctcgtcggc gtccctcgtc ttcgggggct tcgccgagcc taaactggct atgatcgaaa
     1321 ggctgcgcag accagtcggg cccacccctc ggctgcgcca gcaagcgccg aaatacggcc
     1381 gctcgcgcat ccttatggga gttacgagga gtgcatatga agcaagagct gaaccctggc
     1441 cgcatcggcc gcgtcgcatt gctggcggcg ctgtttgcct gcggtcccgc tttcgcgcaa
     1501 tcgtctctcg acatcggcac ctccgtgccg gacgctgcct cggtgaagga cggcctcttt
     1561 cccgaagacg cctgcaagga actcgaggcc agcggcttca agtgcatggg cctgaagccg
     1621 gcggtcaaat attcgctgcc ggcgtcgtcg ttcaaggtcg gctccgccga gctcccggag
     1681 ctgctgaaga agcagctcga cgtcttcgcc gaggtgctgc gatcgaagaa gggcaccgcc
     1741 cggcagatca aggtcaccgg ccatgccgac gcctccggca cgcccgaagc gaatcagcag
     1801 ctttcgatcc ggcgggccga agccgtcaag gaatacctgg tgcaaaaggg cgccgacccg
     1861 aacatgctga cgatctacgg ccagggctcc aaggatccgg tcaacccggc cgatccgctc
     1921 gcgccggaga atcgccgggt cgaaatcggc cgctgaggcg gttcgtcccc tagcggaaac
     1981 ggccggtttc tcccggccgt tcctcattgg cgccggcggc gcggcctcag gggatctgct
     2041 cggtcacctg gccggcgaag ttgaccttga tgacgccgcc ccacatgcag ttcaggatgg
     2101 aggtgtcgtc gagcgccggc tggttggcga gcatcacgtt cggcgcgccg ggcgcccacg
     2161 gcgtggtggt attgggaatg cacggcatgg gggtgagcac gcccagcgcc gccgccgtcg
     2221 ccgcggcgac gaccggattc gaggggctgc tgcacatccc gaacggcatg atgttgacca
     2281 tcggcttgtt gtccatgatg ttcgctgccg gcacgaacga ggtcagcacc ttgttgaccg
     2341 gaagcaccac cagcgagctc ggtgcgacgc cgaagctgca ctgcagcaag gcgcccatgc
     2401 agacgtgatt cgccatcctt tcctccgctt cctcagggac cgggctcgct gacccagacg
     2461 gcgagcgcgg tgaagttgtc gtgccgcggc ttgtgcgagg cgccgtgcag cacggcctgc
     2521 tcgagcacct tcagccaggc gctcgggctg acggcctggg tcagcgcggc ttccatctgc
     2581 gcatcctcga cgtactccca catgccgtcg gtgcagagca ggaacgcgtc gcccgcctcg
     2641 acctgccacg gcggcgccga gacgctgacc tgcaggtctt cctcgcccac gccgagggcc
     2701 gaaagcagtt cgctgcgctg cggatgcgag cgcatgtcct cggcggcgag catgccgccg
     2761 tcgaccagcg actgcaccag gctgtggtcg cgggtgcgcg ccgccacctt gccggcgcga
     2821 aagacgtaga gccgcgagtc gccgcagtgg ccccaggtgc cggcccgggc ctcgaagtcg
     2881 atgaacaggg cgacgaccgt gctgtgcatg ttctgcagcg cacggtcgtc ggcgcgatgc
     2941 tggatgatcg agcggttggt cgcgacgatc atccgctcga tcgcctcggg cgtgccatcc
     3001 ggggcgccgg cgaaggcgcc gaggatgaac tgcaccgcca gcttcgaggc cttgtcgccg
     3061 ccgccgtgac cgccggcgcc gtcggcgacg acgcagcaca gatggctgtc cgagtgccag
     3121 tggccgcagg cgtcctcgtt gtagttgcga ccgccccggc gcgacagggt cgcgagttcc
     3181 atctgcacga tcaaggtctc ccgctacgcc cgcggcgcgt tgccatcatg gccgatcctt
     3241 ggccagctgg tcgatgtact cgttgtaggc gcgcaggaac tccttgccga aaagttcatg
     3301 gaaatcgtcg gaagcttcgg acgagatctg gctgtacaac tgggtgaaca cctcccacat
     3361 ctgcgccttg ctctggcccg gcaggatcga ggagagcagt gatttctggg tgagcttgcc
     3421 ttccagcgtc gccgggtcga agcgcttcaa gatgccttcg agcgcggcgc gcatgccggc
     3481 gagaaagccg aactggtgcg cgcgcaggtc gtcgtaggcg tcgcgcatcg ccttgtcgga
     3541 ttccatgaag ccgcgcgctg gcgggctgag caggtggccg agcgccacct ccaccgaggg
     3601 cgagaacttg agcgggttgt tctcccgggt gacgatcatc gtcacctcgg cgcgaatctc
     3661 g
//
LOCUS       DASRDG010000673         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_336502, whole genome shotgun sequence.
ACCESSION   DASRDG010000673 DASRDG010000000
VERSION     DASRDG010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336502"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFA35_11330"
     CDS             complement(<1..450)
                     /locus_tag="VFA35_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56811.1"
                     /translation="MHPGPRDVAQIRLANALILFDEFVHATVKHADAATLRGLEGRFA
                     ERLQIQPSYWSQIKGRSRQIGERLARQFEQLCKKPAGWMDEHHGPHEWANRGTDTPQP
                     GARVTPAALAASDDDRQRNVPQDDDERFIVGLVLSYYRRHPQRARARL"
     gene            535..1737
                     /gene="purT"
                     /locus_tag="VFA35_11335"
     CDS             535..1737
                     /gene="purT"
                     /locus_tag="VFA35_11335"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HZT56812.1"
                     /translation="MTTLGTPLSPHATKVMLLGSGELGKEVLIALQRLGVETIAVDRY
                     VDAPGQQVAHHARTIAMSDPAQLQALIETERPMLVVPEIEALATSTLEALEAAGVVRV
                     IPTARAARLTMDREGIRRLAAETLALPTSPYRFCDSLAELQAAIDTAIGYPCIVKPVM
                     SSSGKGQSRIDGPADVSKAWEHAMAGGRVSHSRVIVEGFIDFDYEITLLTVRALGAEG
                     RVETHFCEPIGHVQVGGDYVESWQPHPMRPAALQRAREIAQAVTDNLGGQGLFGVELF
                     VKGDAVWFSEVSPRPHDTGMVTMITQWQNEFELHARAILGLPVDTALKSPGASAVIYG
                     GVDARGPVFDGVDAALRVPHTELRLFGKPESFVKRRMGVALAFDADVEVARSRAKQAA
                     ACVKPRAM"
     gene            1911..2309
                     /locus_tag="VFA35_11340"
     CDS             1911..2309
                     /locus_tag="VFA35_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56813.1"
                     /translation="MTTDGWWILVGLALLVGAAAGGWLTWRIARMRHAVQLRKVNGEL
                     QRKFLGTIDQLRAAQVRAQTELEQARNAAKRQLAASDAPRAAAARAEERLRAAQIEMD
                     QMRRAKPRPDTEREELTDGFAATRPMYDRL"
     gene            complement(2382..>2888)
                     /gene="fusA"
                     /locus_tag="VFA35_11345"
     CDS             complement(2382..>2888)
                     /gene="fusA"
                     /locus_tag="VFA35_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430570.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZT56814.1"
                     /translation="GGTIPTQFIPAVEKGVRSALDSGVIAGHPLKDVRVIVYDGKSHS
                     VDSKDIAFATAGRKAFIDAVKKAGPIVLEPIVRVEITAPEHSMGDVTGDLSAKRGQVN
                     GTQALAGGAFVVNGQVPLSELSNYQARLNGMTGGQGRYTLELSHYEAVPPSVQAALMS
                     QYQVKEED"
BASE COUNT          434 a         1007 c         1013 g          434 t
ORIGIN      
        1 caggcgtgcc cgcgcgcgtt gcgggtggcg gcggtagtag ctcagcacca ggccgacgat
       61 gaagcgctcg tcgtcgtcct gcggcacgtt gcgctggcgg tcgtcgtcgc tcgcggccag
      121 cgccgccggc gtgacgcgtg cgccgggctg cggcgtgtcg gtcccgcggt tcgcccattc
      181 gtgtgggccg tggtgctcgt ccatccagcc cgcgggcttc ttgcacagct gctcgaactg
      241 ccgcgccagc cgctcgccga tctggcgcga gcgacccttg atctggctcc agtagctcgg
      301 ctggatctgt agccgctcgg cgaaacgccc ttcgaggccg cgcagcgtgg cggcatcggc
      361 gtgcttgacg gtggcgtgga cgaattcgtc gaagaggatc agggcgttcg cgaggcgaat
      421 ctgggcgacg tcgcgcgggc cggggtgcat gtgtcagatg ataaagcgtc gttgtgcagg
      481 ggcagcgagg ggagaatccg cggttttcca tctccagttc acaggacgcg ctcgatgacc
      541 acgctcggaa ccccgctctc accccacgcc acgaaggtca tgctgctggg cagcggcgag
      601 ctcggcaagg aggtgctgat cgcgctgcag cggctcggcg tcgagacgat cgcggtcgat
      661 cgatacgtcg acgcgccggg ccagcaggtc gcgcaccacg cgcgcacgat cgcgatgagc
      721 gatcccgcgc aactgcaggc gctgatcgag acggagcgcc cgatgctggt cgtgcccgag
      781 atcgaggcgc ttgcgaccag cacgctcgaa gcgctcgaag cggccggcgt cgtgcgcgtg
      841 attccgaccg cgcgcgccgc gcgcctgacg atggaccgcg agggcatccg ccgcctcgcc
      901 gccgagacgc tggcgctgcc gaccagtccg taccggttct gcgactcgct ggccgagctg
      961 caggcggcca tcgacacggc catcggctac ccgtgcatcg tcaaaccggt gatgagcagc
     1021 tccggcaagg gccagagccg gatcgacggc ccggccgacg tcagcaaggc gtgggagcac
     1081 gcgatggccg gcggccgcgt cagccacagc cgcgtgatcg tcgagggttt catcgacttc
     1141 gactacgaga tcacactgct cacggtgcgc gccctcggcg ccgagggccg ggtcgagacc
     1201 cacttctgcg agccgatcgg ccatgtgcag gtcggcggcg actatgtcga gagctggcag
     1261 ccgcacccga tgcgcccggc cgcgctgcag cgtgcgcgcg agatcgcgca agccgtgacc
     1321 gacaacctcg gtggccaggg cctgttcggc gtcgaactct tcgtcaaggg cgacgccgtc
     1381 tggttcagcg aggtctcgcc gcgcccgcac gacaccggca tggtcacgat gatcacgcag
     1441 tggcagaacg agttcgagct gcacgcgcgc gcgatcctcg ggctgccggt cgacaccgcg
     1501 ctgaagagcc cgggcgcgag cgcggtgatc tatggcggcg tcgacgcccg gggcccggtg
     1561 ttcgacggtg tcgacgcggc gctgcgcgtg ccccacaccg agctgcgcct gttcggcaag
     1621 cccgagagct tcgtgaagcg ccgcatgggc gtggcgctcg cgttcgacgc cgatgtcgag
     1681 gtcgcgcgca gccgggccaa gcaagctgcc gcatgtgtga agccgcgtgc gatgtaggcg
     1741 ccgggcagcg cccgaaatga ctgcggggtg cgcgcggtgt gcggtttgca cgcccgattc
     1801 gtcgcgccgc acacgctgcg ctgcagtttc gatcgctcgg tgccgataag ttgctaggct
     1861 gcccgccttg cgtggcggca cgcgacccgc gtcacccgag agaacgacac atgacaacgg
     1921 acggatggtg gatcctggtc ggcctcgcac tgctcgtcgg tgccgccgcg ggcggctggc
     1981 tgacttggcg cattgcgcgg atgcgccacg ccgtgcaact gcgcaaagtg aacggcgaac
     2041 tgcagcgcaa gttcttgggc acgatcgacc aactgcgcgc cgcgcaggtg cgcgcccaga
     2101 ccgaactcga acaggcccgc aacgcggcca agcgccagct cgcggcgtcc gatgcaccac
     2161 gcgccgccgc ggcgcgtgcc gaagagcgct tgcgcgccgc ccaaatagag atggatcaga
     2221 tgcgtcgcgc caagccgcgg cccgacaccg agcgcgagga actgaccgac ggcttcgccg
     2281 ccacgcgacc gatgtacgac cggctctgag gtagccgtct cagccggcgc cgggccgtcc
     2341 caaggcggcc gagcgcccca agggccacga aggggccgtc atcagtcctc ttccttcacc
     2401 tggtactggc tcatcaacgc cgcctgcacc gagggcggca ccgcctcgta gtggctcagc
     2461 tccagcgtgt agcggccctg cccgccggtc atgccgttca gacgcgcctg gtagttcgac
     2521 agctccgaca gcggtacctg cccgttcacg acgaacgccc cgccggccag cgcctgcgtg
     2581 ccgttgacct gcccgcgttt ggccgacaga tcgccggtca cgtcgcccat gctgtgctcg
     2641 ggcgcggtga tctcgacccg aacgatcggc tcgagcacga tcgggcccgc ctttttcacc
     2701 gcgtcgataa aggccttgcg cccggcagtc gcgaacgcga tgtccttcga gtcgacgctg
     2761 tgcgacttgc cgtcgtagac gatcacgcgc acgtccttca acgggtgacc ggcgatcacg
     2821 cccgagtcga gcgccgagcg cacgcctttc tcgaccgccg ggatgaactg ggtcgggatc
     2881 gtgccgcc
//
LOCUS       DASRDG010000674         3099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1366994, whole genome shotgun sequence.
ACCESSION   DASRDG010000674 DASRDG010000000
VERSION     DASRDG010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366994"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFA35_11350"
     CDS             complement(<1..956)
                     /locus_tag="VFA35_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZT56815.1"
                     /translation="MAKSHADARLLLSPGLKDAPVLDRMCSQFVLTLTMHNAGRFNLR
                     RDWNSLLSLTGKHLVWPGSVLARLRGFLKTRCKGNEHWRGHELLDDDAFMLRHGGWRG
                     PYEEGTLFFYIDEYIKDAPKDLLAVLGATADWLGRSLKKESTLVQKNIDSLAGLLQLN
                     PAERALLLYGTLARYQRDLRGLLVEFKVSNAQEAYAAIANVAGVSETDVAEALRAGSR
                     LERIGMVENLISEHNITDLADLMKVSEKLPPVLMREYRDQNELMAVFTRPSNKSELKL
                     SDFEFVADDAQVLCSLLRNAVTRKEAGVNVLLYGPPGTGKTEL"
     gene            complement(1170..1463)
                     /locus_tag="VFA35_11355"
     CDS             complement(1170..1463)
                     /locus_tag="VFA35_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HZT56816.1"
                     /translation="MQMLYNSDSYAVVLFDVAAAVAPGAAPRVADAPEPLSHGGYEIV
                     DKFARKEIFLQGAMAESFKAGVEALIQTKPSEEEIDDYIERFTSLMQQPVILH"
     gene            complement(1580..2590)
                     /locus_tag="VFA35_11360"
     CDS             complement(1580..2590)
                     /locus_tag="VFA35_11360"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HZT56817.1"
                     /translation="MSTHGFGPNFLPRLGVPIGAALLLHAVILGGADWLWPSTAPPPL
                     PTAALQVRVVAPAPPVATQARAVVAVPPPVAPVAARVKPVPPPIAVARTAAVTVERRT
                     AVVAAAPVAAPADDAAIPLYRTRLPPAATLRYELSRGPLRGTGELVWRPQGDRYELRL
                     EARLDGLTILAQTSTGALDAAGIAPLRFTDQRPRRGTLAANFQREAGKISYSGSASEF
                     AVQPGAQDRLSWMLQLAAIVSAEPQLALAGAKVVMQVTGARGDAGVWVFRCLGPEALD
                     TRGGATDALKFVREPREAYDTTVQVWLDAKPPHFPLRATQNTGGSDVGYELRLVEASV
                     PN"
     gene            2630..>3099
                     /locus_tag="VFA35_11365"
     CDS             2630..>3099
                     /locus_tag="VFA35_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZT56818.1"
                     /translation="MKLATYQDGSRDGQLVVVSRDLTLAHYASHAATRLQQVLDDWSF
                     LSPQLEEISQTLNHGKARHAFTFEPEKCMAPLPRAYQWADGSAFINHVELVRKARGAE
                     MPESFYTDPLMYQGGSDDLLGPRADIVAASAEHGIDFEAEVAVVTGDVAMGSSP"
BASE COUNT          475 a         1095 c         1055 g          474 t
ORIGIN      
        1 agctcggtct tgcccgtgcc cggcgggccg tacaacagca cgttcacgcc cgcctccttg
       61 cgcgtgacgg cgtttcgcaa cagcgaacac agcacctggg cgtcgtcggc gacgaactcg
      121 aagtcggaca gtttcagttc gctcttgttc gacgggcgcg tgaacacggc catcagttcg
      181 ttctggtcgc ggtactcgcg catcagcacc ggcggaagtt tctcgctcac cttcatcagg
      241 tcggcgaggt cggtgatgtt gtgctcggag atcaggttct cgaccatgcc gatgcgctcc
      301 aggcgcgagc cggcgcgcag cgcctcggcg acgtcggtct cgctgacgcc ggcgacgttc
      361 gcgatcgcgg cgtatgcctc ctgcgcgttc gagaccttga actcgaccag cagcccgcgc
      421 aggtcgcgct ggtagcgcgc gagcgtgccg tacagcagca gcgcgcgttc ggccgggttc
      481 agctgcagca gcccggccag cgagtcgatg ttcttctgga ccagcgttga ctccttcttc
      541 aggctgcggc ccagccagtc ggccgtggcg ccgagcacgg cgagcaggtc tttcggtgcg
      601 tccttgatgt actcgtcgat gtagaagaac agcgtgcctt cttcgtaggg cccgcgccag
      661 ccgccgtggc gcagcatgaa ggcgtcgtcg tcgagcagct cgtgaccgcg ccaatgctcg
      721 ttgcccttgc agcgcgtctt caggaagccg cgcagccgcg ccagcaccga gccgggccag
      781 acgaggtgct tgccggtcag cgagagcagg ctgttccagt cgcgccgcag gttgaagcgc
      841 ccggcgttgt gcatggtcag cgtcagcacg aactgcgagc acatgcggtc gagcaccggc
      901 gcatccttca ggccgggcga cagcagcaag cgtgcatcag cgtggctctt ggccatgaca
      961 cctccgtggg cggtagggcc tgctaacgct acgggagggc atcggccctt cgggtgacct
     1021 cccgtagcgt tagcaggccc aagtcggtgg atcatcttgg cgcagagtgc gcagcggggc
     1081 aagccccggc gcgcggcacc gttgacctta acgccggcgg gcgcgatcgg aggacacccg
     1141 gcgccccggc cggcgcgcca agtgtcggtt caatgaagga tgacgggctg ctgcatcagc
     1201 gacgtgaagc gctcgatgta gtcgtcgatc tcttcctccg agggcttggt ctgaatcagc
     1261 gcctcgacgc cggccttgaa gctctcggcc atcgcgcctt gcagaaagat ttccttgcgc
     1321 gcgaacttgt cgacgatctc gtagccgccg tggctcagcg gctcgggcgc atcggccacg
     1381 cgcggggcgg caccgggcgc cactgcagcc gccacgtcga acagcaccac ggcgtagctg
     1441 tcggagttgt agagcatctg cattcggtgg ttccttcgtg agcgccttgc attcgatcga
     1501 tgacaactac ctgagcattg caacgcaaat ttcaagccaa cggatgaccg cgcccgggac
     1561 tttcccccga gccagggggt caattcggca cgcttgcctc gaccagccgc agttcgtaac
     1621 ccacgtcgct gccgcccgtg ttctgggtcg cgcgcagcgg gaagtgcggc ggcttcgcgt
     1681 cgagccagac ctgcaccgtg gtgtcgtagg cctcgcgcgg ctcgcgcacg aacttgagcg
     1741 cgtcggtcgc gccgccgcgg gtgtcaaggg cctccgggcc gaggcagcga aatacccaca
     1801 cgcccgcatc gccgcgcgcg ccggtgacct gcatcacgac ctttgcgccc gccagcgcga
     1861 gctgcggctc ggcggaaacg attgccgcga gctgcagcat ccagctgagc cggtcctgcg
     1921 cgccgggctg caccgcgaat tcgctcgccg agcccgagta gctgatcttg ccggcctcgc
     1981 gctgaaagtt cgccgcgagc gtgccgcgcc gcgggcgctg gtcggtgaag cgcagcggcg
     2041 cgatgccggc cgcgtcgagc gcgccggtgc tggtctgcgc caggatcgtc aggccgtcga
     2101 gcctggcctc gagccgcaac tcgtagcgat cgccttgcgg gcgccagacc agctcgccgg
     2161 tgccgcgcag tgggccccgg ctgagctcgt agcgcagcgt cgcggccggc ggcagccgcg
     2221 tgcggtagag cggaatcgcc gcgtcgtcgg cgggtgcggc gacgggcgcg gcggccacca
     2281 ccgccgttcg tcgttcgacg gtcaccgccg ccgttcgcgc cacggcgatc ggcggcggca
     2341 ccggcttgac ccgcgccgcc accggcgcaa ccggcggcgg cacggcaacc accgcgcgcg
     2401 cctgggtcgc caccggcggc gcgggcgcca cgacgcgcac ctgcaacgcc gccgtcggca
     2461 ggggcggcgg cgccgtcgac ggccagagcc aatccgcgcc acccaggatc acggcatgca
     2521 gcagcagcgc cgcgccgatc ggcacgccca ggcgcggcag aaaattcgga ccgaagccgt
     2581 gcgtcgacaa tggcgttcct tcgacaatcg cagcgcctgc gggacaacca tgaagctggc
     2641 aacctaccaa gacggctcgc gcgacggcca actcgtcgtc gtctcgcgcg acctgacact
     2701 cgcccactat gccagccacg ccgcgacccg gctgcagcag gtgctcgacg attggagctt
     2761 tctctcgccg cagctcgaag agatctcgca gacgctgaac cacggcaagg cgcgccacgc
     2821 gttcacgttc gaacccgaga agtgcatggc gccgctgccg cgcgcctacc aatgggccga
     2881 cggctcggcc ttcatcaacc acgtcgagct cgtgcgcaag gcgcgcggcg ccgagatgcc
     2941 cgagagcttc tacaccgacc cgctgatgta ccaaggcggc agcgacgacc tgctcggccc
     3001 gcgcgccgac atcgtcgccg ccagcgccga gcacggcatc gacttcgagg ccgaggtcgc
     3061 ggtggtcacc ggcgatgtcg cgatgggcag ctcgcccga
//
LOCUS       DASRDG010000675         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1367180, whole genome shotgun sequence.
ACCESSION   DASRDG010000675 DASRDG010000000
VERSION     DASRDG010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367180"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFA35_11370"
     CDS             <1..343
                     /locus_tag="VFA35_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZT56819.1"
                     /translation="DDPDAPDPAAPTRTWVHWVLVDIPPGAIAIPEGGGPLPEGCRDG
                     LNDWQRTGYGGPCPPVGRHRYFFKLYALDTRLPGLQRPTKADLERAMQGHVLAQAQMV
                     GTYQKHKGGAG"
     gene            407..622
                     /locus_tag="VFA35_11375"
     CDS             407..622
                     /locus_tag="VFA35_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56820.1"
                     /translation="MTHEQLAAHDDKGERYVVMVTRTPIPGSPHLKGPPRYTWRDGQA
                     LHLIDAKAGILECVVTGQRLKIEAWRG"
     gene            complement(750..>2753)
                     /locus_tag="VFA35_11380"
     CDS             complement(750..>2753)
                     /locus_tag="VFA35_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HZT56821.1"
                     /translation="LGDAFASGGSAGYDHKVMGITARGAWESVKRFFREQGIDTQSTP
                     FTVVGVGDMSGDVFGNGMLLSRHIRLVAAFDHRHVFIDPNPDPATSFAERERLFKLPR
                     SSWADYDAKLISDGGGIWARSEKSIPISPQTRATLGIEAERLTPTELLSAILKAPVDL
                     LYNGGIGTYVKASTETHADVGDRANDALRIDGRELRCKVVAEGGNLGFTQRGRIEAAL
                     AGVRLNTDAIDNSAGVDTSDHEVNIKILLGLAISDGEMTEKQRNALLAQMTDDVAMLV
                     LRDNYFQAQALSVGARLGPSYLDQEVRFIRFLEKTGNLNRAIEFLPAEDEIAERKARG
                     AGLTTPERAVIFAYSKMWLFEEIMASDLPEDPWIGTALARYFPALLREKFGAYIPRHP
                     LKREIVATHVLNSMINRVGSTFVHRLSERTGARPAQVVRAYLAAREVLGDVAVWQQVE
                     ALDNKVPDAVQSEMLIEEGWLTARATTWFLRSRRLAEPLEQTLRRFIPAVQALRARLE
                     PEAAASPQATAWIAAGAPPPLALRVASADHLIAALDVAEVAEAVQRNFDEVSEVHVGI
                     GTRLGLSRLRQQIDELPSDSHWQSLAKAALGDDLAGLQHTLAQDVLQQGPGSPAELLA
                     TWETGNATALERAQRLLAELADAKGGADLSMLSVALRELRNLG"
BASE COUNT          411 a          940 c          983 g          419 t
ORIGIN      
        1 cgatgacccg gacgcgccgg acccggcggc accgacccgg acctgggtgc actgggttct
       61 ggtcgacatt ccgcccggcg cgatcgcgat tcccgagggt ggcgggccgc tgcccgaagg
      121 gtgccgggac ggcttgaacg actggcaacg gaccggctat ggcgggccct gtccgccggt
      181 ggggcggcac cgctacttct tcaagctgta cgcgctcgat acacggctgc ccggtctgca
      241 gcggccgacc aaggccgacc tcgagcgcgc aatgcagggc catgtgctcg cgcaggccca
      301 aatggtcggg acctaccaga agcacaaagg aggagccggg tgaggccatc acggccccgg
      361 cttgggacct ggcgcgcacc aacggaacga cgacatgcca aacagaatga cccatgagca
      421 actggccgcc cacgacgaca agggcgagcg ctacgtcgtc atggtgaccc gcacgccgat
      481 cccgggctcg ccgcatctca agggaccgcc gcgctacaca tggcgcgacg gacaagcgct
      541 gcacctcatt gacgcgaagg ccggcatcct cgagtgcgtc gtcaccgggc agcgattgaa
      601 gatagaggcc tggcgcggat gagacccgcg ctgattcccc aaggtccttg caaactgcgc
      661 ggagcgaacg cagccgttcc tcggttcgct tccaggctca cctcatctgc aaggaccgcc
      721 tcacatgggg tgccggacct tccctgacac tagccgaggt tgcgcagctc ccgcagcgcc
      781 accgagagca tcgacaggtc ggcgcctccc ttggcgtcgg ccaactcggc gagcagccgc
      841 tgcgcgcgct cgagcgcggt cgcgttgccc gtctcccagg tcgccagcaa ctcggcgggg
      901 ctgcccgggc cctgctgcag cacgtcctgg gcgagcgtgt gctgcagccc ggcgaggtcg
      961 tcgccgagcg cggccttcgc cagggattgc cagtggctgt cggacggcag ttcgtcgatc
     1021 tgctggcgca gccgcgagag gccgaggcgg gtgccgatgc cgacgtgcac ctcgctcacc
     1081 tcgtcgaagt tgcgctgcac cgcctcggcg acctcggcga catcgagtgc ggcgatgagg
     1141 tggtcggcgc tggccacgcg cagggccagc ggcggcggcg cgcccgcggc gatccaggcg
     1201 gtcgcctgcg gcgacgcggc ggcctccggc tcgagccgcg cgcgcagcgc ctgaaccgcc
     1261 gggatgaagc ggcggagcgt ctgctccagc ggctcggcca ggcgccgcga gcgcaggaac
     1321 cacgtcgtgg cgcgcgcggt gagccagccc tcctcgatca gcatctccga ctgcacggcg
     1381 tcgggcacct tgttgtcgag cgcctcgacc tgctgccaga cggcgacgtc gccgagcacc
     1441 tcgcgcgctg ccaggtaggc gcgcaccact tgcgccggcc gcgcgccggt gcgctccgac
     1501 aggcgatgca cgaaggtcga accgacccgg ttgatcatgc tgttgagcac gtgcgtggcc
     1561 acgatctcgc gcttgagcgg atggcgcggg atgtaggcac cgaacttctc gcgcagcagc
     1621 gccgggaagt agcgcgccag cgcggtgccg atccaggggt cttccggcag gtcggaggcc
     1681 atgatctcct cgaacagcca catcttgctg taggcgaaga tcaccgcccg ctcgggggtg
     1741 gtgaggccgg cgccgcgcgc cttgcgctcg gcgatctcgt cttcggccgg caggaactcg
     1801 atggcgcggt tcaggttgcc ggtcttctcg aggaagcgga tgaagcgcac ctcctggtcg
     1861 aggtagctcg gaccgaggcg cgcgccgacc gacagcgcct gcgcctggaa gtagttgtcg
     1921 cgcagcacca gcatggcgac gtcgtcggtc atctgcgcga gcagggcgtt gcgctgcttc
     1981 tcggtcatct cgccgtcgct gatcgcgagg ccgagcagga tcttgatgtt gacttcgtgg
     2041 tctgaggtat cgaccccggc cgagttgtcg atcgcgtcgg tgttgaggcg cacgccggcg
     2101 agtgccgcct cgatgcgccc gcgttgggtg aagccgaggt tgccgccttc ggccacgacc
     2161 ttgcagcgca gctcgcggcc gtcgatgcgc agcgcgtcgt tagcgcggtc gccgacgtcg
     2221 gcgtgggtct ccgtgctggc cttgacgtaa gtgccgatgc cgccgttgta gagcaggtcg
     2281 acgggcgcct tcaggatcgc actcaacagc tcggtcggtg tcaggcgctc tgcctcgatg
     2341 ccgagggtgg cgcgagtctg tggcgagatc gggatcgatt tctcggagcg cgcccagatg
     2401 cccccgccgt cggagatcag cttcgcgtcg tagtcggccc acgacgagcg cggcagcttg
     2461 aagaggcgct cccgctcggc gaatgacgtg gcggggtcgg ggttggggtc gatgaacaca
     2521 tgccggtggt cgaacgccgc gaccagccgg atgtgccgcg acagcagcat gccgttgccg
     2581 aacacgtcgc ccgacatgtc gccgacgccg acgacggtga acggcgtgct ctgggtgtcg
     2641 atgccctgct cgcggaagaa gcgcttcacg ctctcccagg cgccacgcgc cgtgatcccc
     2701 atgaccttgt ggtcgtagcc ggcgctgccg ccggaggcga aggcgtcgcc gag
//
LOCUS       DASRDG010000676         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_220371, whole genome shotgun sequence.
ACCESSION   DASRDG010000676 DASRDG010000000
VERSION     DASRDG010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220371"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VFA35_11385"
     CDS             <1..1336
                     /locus_tag="VFA35_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HZT56822.1"
                     /translation="DARDFDDAVYAEREGDGFRLLVAIADVSHYVRPGTALDTEARAR
                     GTSVYFPTRVLPMLPTALSDHLCSLEPQVDRLCMAADMSVSARGALRSWRMYPAVMRS
                     AARLTYDMAHTLLFRREPAARAALGALAARLDPLVEVYQVLLRARAQRGALDFDAPEA
                     KFVLSREEQVRAIEFRSRNDAHKLIEECMVLANVAAAKEMRRLHVPGLHRVHPPPEQR
                     KLDALKGTLQVLGIELQLPEQVRTRDLASIASRVRDPELRPMVESLVVRSLAQALYQP
                     DHIGHFGLALPDYAHFTSPIRRYPDLLVHRALRAGLGLAPRESIPDQPALEAAGAELS
                     RLEKRADEADRYVETFLKCVYLRDRVGQSFDGLVTTVVEFGCFIQLLGVGVDGLLHLS
                     ALRDDDYVMTRDGGQWRGRRSGRNLAPGTRLRVLVTAVNPVEGMVDLELAGD"
     gene            1336..2079
                     /gene="rlmB"
                     /locus_tag="VFA35_11390"
     CDS             1336..2079
                     /gene="rlmB"
                     /locus_tag="VFA35_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZT56823.1"
                     /translation="MALERLYGLHAARILLSRAPQRVLRVCLQQGRNDARAAEIEALA
                     RTAGRPVERVDAQRLRGWVGDVAHQGVVAEIEPLRPMTEDEFYEQLGADREPLVLALD
                     GVQDPHNLGACLRTADACGVVAVVVPRDRAATLNAAARKVAAGAAETTPVVTVTNLAR
                     CLRLIKDAGLWIVGADAEAGQKAADADLRGGRCLVLGAEGKGLRELTRRSCDFLVSLP
                     NQGAVESLNVSVAAGMLLYEALRQRRHPG"
     gene            2147..2533
                     /gene="rpsF"
                     /locus_tag="VFA35_11395"
     CDS             2147..2533
                     /gene="rpsF"
                     /locus_tag="VFA35_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZT56824.1"
                     /translation="MRHYEVVFLVHPDQSEQVPAMIERYRGMIAAGSGQVHRLEDWGR
                     RQLCFPIAKVHKAHYVLMNIECDQKTLAELTGAFRFSDAVLRHLVVSMDGPVTEPSPM
                     ARSEEENEARRERDEMGDDDEMSVGS"
BASE COUNT          399 a          883 c          923 g          410 t
ORIGIN      
        1 ggacgcgcgc gacttcgacg acgcggtcta cgccgagcgc gagggcgatg gtttccggct
       61 gctggtcgcc atcgccgacg tcagccacta cgtgcggccc ggcacggcgc tggatacgga
      121 agcgcgcgcg cgcggcacct cggtctactt cccgacgcgg gtgctgccga tgctgccgac
      181 ggcgctgtcg gatcacctgt gctcgctcga gccgcaggtc gaccgactgt gcatggcggc
      241 cgacatgagc gtgagcgcgc gtggtgcgct gcgcagctgg cgcatgtatc cggccgtcat
      301 gcgctcggcc gcgcgcctca cctacgacat ggcgcacacg ctgctgttca ggcgcgagcc
      361 cgccgcccgc gcggcgctcg gtgcgctggc ggcgcggctc gaccccctgg tcgaggtcta
      421 ccaggtgctg ctgcgggcgc gcgcgcaacg cggcgcgctc gatttcgacg cgcccgaggc
      481 gaagttcgtg ctgagccgcg aggaacaggt gcgcgcgatc gagttccgct cgcgcaatga
      541 cgcccacaag ctcatcgagg agtgcatggt gctggccaac gtcgccgccg ccaaggaaat
      601 gcggcggctg cacgtccccg ggctgcaccg cgtgcacccg ccacccgagc agcgcaagct
      661 cgatgccctc aagggcacgc tgcaggtgct gggcatcgag ctgcagttgc ccgaacaggt
      721 caggacgcgc gacctggcct cgatcgcatc gcgggtgcgt gaccccgagc tgcggccgat
      781 ggtcgagtcg ctggtcgtgc gctcgcttgc gcaggccctc taccagcccg accacattgg
      841 tcacttcggc ctggcgttgc ccgactatgc ccacttcacc tcgccgatcc gccgctaccc
      901 ggacctgctc gtgcaccgcg cgctgcgggc cgggctggga ttggcgccac gcgagtcgat
      961 cccggaccag ccggccctcg aggccgccgg cgccgaactg tcgcgactcg agaagcgggc
     1021 cgacgaggcc gatcgctacg tcgagacctt tctcaagtgc gtctatttgc gcgaccgcgt
     1081 cggccagtct ttcgatgggc tcgtcaccac ggtggtggaa ttcggttgct tcatccagct
     1141 tttgggcgtg ggcgtcgacg ggctgctgca cctcagtgcg ctgcgcgacg acgactatgt
     1201 gatgacgcgc gacggcgggc aatggcgcgg ccggcgcagc ggccgcaacc tggcgcccgg
     1261 gacacggctg cgcgtgctcg tgaccgccgt caatcccgtg gaagggatgg tggacctcga
     1321 gctcgcgggc gactgatggc gctcgagcgc ctgtacggcc tgcacgcggc gcgcatcctg
     1381 ctgtcgcgcg cgccgcaacg cgtgctgcgg gtctgcctgc agcaaggccg caacgatgcg
     1441 cgggcggcgg agatcgaagc cctggcgcgg actgcgggac gtccagtcga acgtgtcgat
     1501 gcgcagcggc tgcgcggctg ggtgggcgat gtcgcgcacc agggtgtcgt tgcagaaatc
     1561 gaaccgctcc ggccgatgac cgaggacgag ttctacgagc agctcggtgc cgaccgcgag
     1621 ccgctcgtgc tggcgctcga tggcgtgcag gatccacaca acctcggtgc ctgccttcga
     1681 actgccgacg cttgtggcgt ggtggccgtg gtcgtgccgc gcgatcgggc cgcaacgctc
     1741 aatgccgccg cgcgcaaggt ggcggcaggc gcagccgaaa cgacgcccgt ggtcaccgtc
     1801 accaacctcg cgcgctgcct gcgtctcatc aaggacgcgg ggctgtggat cgtcggcgcc
     1861 gatgccgaag ccggacagaa ggccgcggat gccgacctcc ggggcggccg ctgcctggtg
     1921 ctgggtgcgg agggcaaggg actgcgggaa ctgacgcgcc gcagttgcga cttcctggtg
     1981 agcctaccga accagggcgc ggtggagagc cttaacgtct cagtcgcggc cggaatgctc
     2041 ctctacgagg ccttgcgcca gcgccgacac cccggctaaa ctgcgcggct tctccttgcc
     2101 gcactgccaa tgcctggcgg gcggcaattt ccataaggag tcatgcatga ggcattacga
     2161 agtggtgttc ctggtccacc cggaccagag cgagcaggtc ccggcgatga tcgagcgcta
     2221 ccgcggcatg attgccgccg gcagcgggca ggtgcatcgc ctcgaggact ggggccgtcg
     2281 ccagctgtgc ttcccgatcg ccaaggtgca caaggcacac tacgtgctca tgaacatcga
     2341 gtgcgaccag aagacgctcg ctgagctgac cggggcattc cgtttcagcg acgcggtgct
     2401 gcgccacctc gtggtcagca tggacggtcc ggtgaccgaa ccctcgccga tggcgcgcag
     2461 cgaggaagaa aacgaggccc gtcgcgagcg cgacgaaatg ggcgacgacg acgaaatgtc
     2521 ggtcggctcc tgaagcattg agggagaaca agatgtctcg tttcgttcgc cgcaagaaat
     2581 tctgtcgctt cacggctgat gcggtcgcca cgatc
//
LOCUS       DASRDG010000677         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_678016, whole genome shotgun sequence.
ACCESSION   DASRDG010000677 DASRDG010000000
VERSION     DASRDG010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678016"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VFA35_11400"
     CDS             complement(<1..998)
                     /locus_tag="VFA35_11400"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZT56825.1"
                     /translation="MDAPAVRLHLFGSPTLESGEARTALSLERRTQLVVLLALRRQWV
                     PRAEIAAMLWPEQEDRLAFANLRKTLFRLQALPWAAAIETQGAALRLEMATDVADFES
                     ALREQRSAEALDAYRGELLAGFDDGESEAWTRWVAFERDRLRTAWRAAALARLADPAL
                     EPAQAVALSARLLEVDPLDEAALRQHMSALARDGQAGAARAAYRQFVERLSTDLGLTP
                     GAELRAMHDSLLGARPVASGIGVPAAGPADDGFVGRSVELQRIADLLARDECRLLCLI
                     GPGGVGKTRLARRTMQELAPRYADGAVFVPLEDVDTPEQLGLRLAREGGIAAASGN"
     gene            1066..1959
                     /locus_tag="VFA35_11405"
     CDS             1066..1959
                     /locus_tag="VFA35_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZT56826.1"
                     /translation="MFIAPPTAAAGDATPPPAVERPFRPSPWACGDYAVIGATLAIVA
                     EQLVEAADVRAGEKVLDVAAGTGNAALAAARRYAHVTATDPVRALLDRARERARAEAL
                     PLHCQVGSVEALPFADASFDVVLSTFGATYAPDEASAAREMLRVLRPGGRLGLASWTP
                     RGFVGRLCRVVGAHLPPVDRCTSPTRWGTPPRLAALSGLPPSQLQCRWRRFDFRYRSA
                     AHWVQVFRDFHGPLHQAFAALDPPRQQALEHAITALLQAANTAGPGSLVVPASYLEAV
                     ITRPPIPSPAAHPRSDSSPPP"
BASE COUNT          236 a          789 c          714 g          264 t
ORIGIN      
        1 tcgttgcccg aggcggcggc gatcccgccc tcgcgggcga ggcgaagtcc gagctgctcc
       61 ggcgtgtcga cgtcctcgag cggcacgaag acggcgccgt cggcgtagcg cggcgccagc
      121 tcctgcatcg tgcgccgtgc cagccgcgtc ttgccgaccc cgccgggacc gatcaggcac
      181 aacaggcggc attcgtcgcg cgcgagcagg tcggcgatgc gctgcagctc caccgagcgg
      241 ccgacgaagc cgtcgtcggc cgggcctgcc gcgggcacgc cgatgccgct cgccaccgga
      301 cgcgcgccaa gcaagctgtc gtgcatggcg cgcagctccg cgccgggcgt caggccgagg
      361 tcggtcgaca gccgctcgac gaactgccgg tacgccgcgc gcgccgcacc ggcctggccg
      421 tcgcgcgcca gcgcgctcat gtgctgtctg agggcggctt cgtcgagcgg gtcgacctcg
      481 agcagccggg ccgacagcgc gaccgcctgc gccggctcga gcgccgggtc ggccaggcgc
      541 gccagcgccg cggcacgcca ggccgtgcgc aggcggtcgc gctcgaacgc gacccagcgc
      601 gtccacgctt cgctttcgcc gtcgtcgaat ccggccagca gttcgccgcg gtaggcgtcc
      661 agcgcttcgg cgctgcgctg ctcgcgcagc gccgactcga agtccgcgac gtcggtcgcc
      721 atctcgaggc gcagcgccgc gccttgcgtc tcgatcgccg ccgcccatgg cagcgcctgc
      781 aggcgaaaca gggtcttgcg caggttggcg aaggcgagcc ggtcttcctg ctcgggccag
      841 agcatcgccg cgatctcggc ccgcggcacc cactggcggc gcagggccag cagcacgacc
      901 agctgggtgc ggcgctcgag ggaaagcgcc gtccgggcct cgccggactc gagcgtcggc
      961 gagccgaaca ggtgcaggcg gaccgccggc gcgtccatgg gaacaagtat cgcagggcgt
     1021 tgccgtggaa cgccggtgga acgccgggct gcgcacagtg ggcccatgtt catcgcaccg
     1081 ccgaccgccg ccgccggcga tgccacgccc ccgccggccg tcgagcgacc gttccggccg
     1141 tcgccgtggg cctgtggcga ctacgccgtc atcggtgcga cgctggcgat cgtcgccgag
     1201 cagctcgtcg aggccgccga cgtccgcgcc ggcgagaagg tgctcgacgt ggccgccggc
     1261 accggcaacg ccgcgctcgc cgcggcgcgg cgctacgcgc acgtgaccgc gaccgacccg
     1321 gtgcgcgcgt tgctcgaccg agcgcgcgag cgggcgcggg ccgaggcgct gccactgcac
     1381 tgccaggtcg gcagcgtcga ggcgctgccc tttgccgacg cgagcttcga cgtcgtgctc
     1441 tcgaccttcg gcgcgacgta cgcgcctgac gaggccagcg cggcccgcga gatgctgcgc
     1501 gtgctgcgcc ccggcgggcg cctcggcctc gcgagctgga cgccgcgggg cttcgtcggg
     1561 cggctctgcc gcgtcgtcgg cgcccacctg ccgcccgtcg accgctgcac ctcgccgacg
     1621 cgctggggca cgcccccgcg actggccgcg ctgtccggcc tgccgccttc gcagctgcag
     1681 tgtcgctggc gtcgcttcga cttccgctat cgctccgcgg cccattgggt gcaggtgttc
     1741 cgcgacttcc acggcccgct gcaccaggcc ttcgccgcgc tcgacccgcc gcgccagcag
     1801 gcgctcgagc acgccatcac ggcgctgctg caggctgcca acaccgccgg tccgggctcg
     1861 ctcgtggtgc cggcttccta tctcgaagcc gtgatcacgc ggccgccgat tccttcgccc
     1921 gcggcgcatc cgcgctccga ttcttcgccc ccgccctgag gaaccttccc gtgtcacgtc
     1981 acgaaaacat cctgcagacc atc
//
LOCUS       DASRDG010000678         3226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_910069, whole genome shotgun sequence.
ACCESSION   DASRDG010000678 DASRDG010000000
VERSION     DASRDG010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910069"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFA35_11410"
     CDS             <1..722
                     /locus_tag="VFA35_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZT56827.1"
                     /translation="FLRVLKDALFAGPGSADLLLPRASLSALLPTPAQRWLGEAGATL
                     RLQQRVDALLADGAGWSVAGERFDHVVLATTPVEAVRLTREIAPGWSRAAAALRYEPI
                     VTVYLQGAGVRLAEPMLTLRSDAHSPAQFVFDRGQLGGPTGLLAFVISGAQPWVDRGI
                     AATAAATIAQAQAALGLHLTLLQALTEKRATFRCTPGLQRPALRIAPNLHAAGDHVAG
                     PYPATLEGAVRSGLAAVSAFG"
     gene            complement(706..1845)
                     /locus_tag="VFA35_11415"
     CDS             complement(706..1845)
                     /locus_tag="VFA35_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HZT56828.1"
                     /translation="MRRSLLRGVGFALLLVATAFTAAAQAGGADYLTRRYDFGPPAAS
                     AASAAPQWIRIPRVAGRITAKELGLVINTADPYSIEVGEFYAKARGLTRAQVLRVQLP
                     VTPTLSPDEFKAFAAQVDAFFDVPIQALALAWVQPYAVHCNSITGALALGYDRALCDH
                     SCAASARSRYFDNGSTRPYSDLKMRPSMLLAARDVAAAKAMIQRGVAADGSLGLRGAV
                     PVNAYFVTTGDAARNVRTPLFPPPGLLRRVGVDVHVEKTEALENASRVLIYQTGAAKV
                     GKLDTIRWVPGALADHLTSYGGALEAANGQMSILDWIGAGATASYGTVSEPCAHPQKF
                     PQPQVLLLNYLQGASALEAYWRSVAWPQQGVFVGEPLAAPFSRTR"
     gene            1916..2683
                     /locus_tag="VFA35_11420"
     CDS             1916..2683
                     /locus_tag="VFA35_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZT56829.1"
                     /translation="MAKTESKTPTIQVLERAFGLLDVLASHPDPVSLKHISEQAGLHP
                     STAHRILNDLTIGRYVDRPEAGSYRLGMRLLELGNLVKARLDVRDAALGPMRELHKLT
                     HQPVNLSMRQGDEIVYIERTYSERSGMQVVRAIGGRAPLHLTSVGKLFLAHDDAPRVR
                     AYATRTGLAGHTKNSITELAQLERELARVNQYGTARDDEELELGVRCMAASILDDQGK
                     LIAGLSISAPADRLEESWLERLKETAAQISAALGHRG"
     gene            complement(2730..>3226)
                     /locus_tag="VFA35_11425"
     CDS             complement(2730..>3226)
                     /locus_tag="VFA35_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S11"
                     /protein_id="HZT56830.1"
                     /translation="ALGRAYPGGLDAFVAAMNRKAKALGMNDTHYVEPTGLSSSNQSS
                     ARDLATLVKAAQEHPIIREFSTSPEFEVEVGNRPTQFRTTNALVRSPAWDIALQKTGY
                     ITEAGRCLVMQARMAGRQLIMVFLDSAGKYSRLGDAERVRRWVSELPVHVPAAAAALP
                     KSTS"
BASE COUNT          477 a         1159 c         1131 g          459 t
ORIGIN      
        1 tgttcctgcg cgtgctgaag gatgccctct tcgccggccc cggctcggcc gacctgctgc
       61 tgccgcgcgc cagtctgagc gcgctgctgc cgacgccggc acagcgctgg ctcggtgaag
      121 ccggcgccac gctgcgcttg cagcaacgcg tcgacgcctt gcttgcggac ggtgccggct
      181 ggtcggtggc gggcgaacgc ttcgaccacg tcgtgctcgc gaccacgccg gtcgaagccg
      241 tacgcctgac ccgcgagatc gcgcccgggt ggtcgcgtgc cgccgccgcg ctgcgctacg
      301 aaccgatcgt caccgtctac cttcaaggcg ccggcgtgcg tctggccgag ccgatgctca
      361 cgctgcgcag cgacgcgcac tcaccggcgc agttcgtgtt cgatcgcggc cagctcggcg
      421 gcccgacggg cctgctcgca ttcgtgatca gcggcgcgca gccctgggtc gaccgcggca
      481 tcgcggcgac ggcggcggcc acgatcgcgc aggcgcaggc ggcgctcggc ctgcacctga
      541 cgctgctgca ggcgctcacc gagaagcgcg cgaccttccg ctgcacgccc gggctgcagc
      601 ggcccgcgct gcgcatcgcg cccaacctgc acgccgcggg cgaccatgtc gccggcccct
      661 acccggccac gctcgaaggc gcggtgcgca gcgggctggc ggcggtcagc gcgttcggct
      721 gaacggcgcc gccagcggct cgccgacgaa cacgccctgc tgcggccagg ccacgctgcg
      781 ccagtaggct tcgagcgcgc tggcgccctg caggtagttc agcagcagca cctgcggctg
      841 tgggaacttc tgcgggtgcg cgcagggctc ggagacggtg ccgtagctcg ccgtcgcgcc
      901 cgcgccgatc cagtcgagga tcgacatctg gccgttcgcg gcttcgagcg cgccgccgta
      961 ggacgtcagg tgatcggcga gcgcgccggg cacccacctg atcgtgtcga gcttgccgac
     1021 cttggccgcc ccggtctggt agatcagcac ccggctcgcg ttctcgaggg cctcggtctt
     1081 ctcgacatgc acgtcgaccc cgacgcgccg caacagcccc ggcggcggga acaagggcgt
     1141 gcgcacgttg cgcgccgcgt cgccggtggt cacgaaatac gcgttcacgg gcaccgcgcc
     1201 gcgcaggccg aggctgccgt cggcggcgac gccgcgctgg atcatcgcct tcgcggcggc
     1261 cacgtcgcgc gccgcgagca gcatcgacgg gcgcatcttc aggtccgagt acggccgcgt
     1321 cgagccgttg tcgaagtaac gcgaacgcgc cgaggccgcg cagctgtgat cgcagagcgc
     1381 acggtcgtag ccgagtgcga gcgcgccggt gatcgaattg cagtggacgg catagggttg
     1441 aacccaggcc agcgccaacg cctgaatcgg cacgtcgaag aacgcatcca cctgcgccgc
     1501 aaaggccttg aactcgtccg ggctcagcgt cggcgtgacc ggcagttgca cgcgcagcac
     1561 ctgcgcgcgc gtcagcccgc gtgccttggc gtagaactcg ccgacctcga tcgagtacgg
     1621 gtcggcggtg ttgatcacga ggccgagttc cttcgctgtg atgcgacccg cgacgcgggg
     1681 aatccggatc cactgcggcg ccgcagacgc cgcggacgcc gccggtggac cgaagtcgta
     1741 gcgtcgtgtc aggtagtcgg cgccgcccgc ctgggccgcc gcggtgaacg cggtggcgac
     1801 gagcagcagc gcgaacccca cgccacgcag caagctacgg cgcattccgc ctcccggatg
     1861 gcagcccgag gtttcgcgat acaaaaatcc gttcagttga gcgagaatcc ggagcatggc
     1921 aaaaactgaa agcaagacgc cgacgataca ggtgctcgag cgcgccttcg ggctgctcga
     1981 cgtgctggcc agccaccccg atccggtctc gctgaagcac atcagcgaac aggccgggct
     2041 gcatccctcc actgcccacc gcatcctgaa cgacctgacg atcggccgct atgtcgaccg
     2101 cccggaggca ggcagttatc ggcttggcat gcgcctgctc gagctcggca acctcgtgaa
     2161 ggcgcggctc gacgtgcgcg acgccgcgct cggcccgatg cgcgagttgc acaagctgac
     2221 gcaccagccg gtgaacctct cgatgcgcca gggcgacgag atcgtctaca tcgagcgcac
     2281 ctacagcgaa cgctcgggca tgcaggtggt gcgggcgatc ggcggccgcg cgccgctgca
     2341 cctcacctcg gtcggcaagc tcttcctcgc gcatgacgac gcgccgcgcg tgcgtgctta
     2401 cgcgacccgc accggcctgg ccgggcacac gaagaacagc atcacggagc tcgcgcagct
     2461 cgagcgcgag ttggcgcgcg tcaaccagta cggcaccgcg cgcgacgacg aggagctgga
     2521 attgggcgtg cgctgcatgg ccgcgagcat cctcgacgac cagggcaagc tgatcgcggg
     2581 cctgtcgatc tcggcgcctg ccgatcggct cgaagagagc tggctggagc gcctgaagga
     2641 aaccgcggcg cagatctcgg ccgcgctggg ccaccgcggc tgaccaccgg ctcgccatgc
     2701 gccgcgcggc gccggcccgg cccggccgat caggacgtcg acttcggcag cgctgcggcc
     2761 gccgccggca cgtgcaccgg cagttcgctg acccagcggc gcacgcgctc ggcgtcgccc
     2821 aggcgcgaat acttgccggc cgagtcgagg aacaccatga tcagctggcg gccggccatg
     2881 cgcgcctgca tcacgaggca gcggccggct tcggtgatgt agccggtctt ttgcagcgcg
     2941 atgtcccagg ccgggctgcg caccagcgcg ttcgtggtgc ggaactgcgt cggccggttg
     3001 ccgacttcga cctcgaactc gggcgaggtc gagaactcgc gaatgatcgg gtgctcttgc
     3061 gcggccttga ccagcgtggc gaggtcgcgc gcgctcgact ggttgctgct cgacaagccc
     3121 gtcggttcga catagtgcgt gtcgttcatg ccgagcgctt tcgccttgcg gttcatcgca
     3181 gcgacgaatg cgtccagacc gcccgggtag gcgcggccga gcgcgt
//
LOCUS       DASRDG010000679         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_910537, whole genome shotgun sequence.
ACCESSION   DASRDG010000679 DASRDG010000000
VERSION     DASRDG010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910537"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VFA35_11430"
     CDS             complement(<1..1075)
                     /locus_tag="VFA35_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56831.1"
                     /translation="MPGGDGQSAHRRHRARRCADGNRQSALRLNLAAARAAAAASPAI
                     MPVSSPAAIEHALLELSALSFVAHREVADALPALRHHGDDVALVWLDAVKRLFQHDRE
                     AGKAFVRGSREAEKISETVLPWTQQALEFLHWPGPAAAVEGYMKNLPRAFGTLGHAGE
                     RRWAEIGFMWYARHSDSGRAYFAMPVLDLACRQGITGIEQLCEPMDELFETRKLMLAT
                     YLGGAIRVRNLLGAQAVLPWALRGADIMQSGRARGEAYFRLESEESLSVLLDHLPGFR
                     MNERNRLLTLLLNVWFERPFELKESTWSPDKGRPFVESDGRSVFVPAVMPDRDEAILA
                     VLHAAGRQLFGSFDRAALEQIFRE"
     gene            complement(988..1833)
                     /locus_tag="VFA35_11435"
     CDS             complement(988..1833)
                     /locus_tag="VFA35_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HZT56832.1"
                     /translation="MKTAATRLRGVEHDIIQQPRASDIAPYYEPQGNEVAIFEAAYRK
                     RLPVMLKGPTGCGKTRFLEHMAFTLKRPLVTVSCHEDLTSSDLVGRFLLEGSSTVWQD
                     GPLTRAVKAGAICYLDEIVEARTDSTVVIHSLTDHRRRLTIEKKGQEIDAHEDFLLVI
                     SYNPGYQTVLKDLKPSTKQRFIAINFDFPAEDVERKIVVNEVSGIDAELAAKLVATGR
                     KGRLLKDHGLEEATSTRALVYAANMINAGLDPVNACQVAMVNPLTDDIELADALMEIV
                     KAHFA"
BASE COUNT          357 a          652 c          634 g          377 t
ORIGIN      
        1 cttcgcggaa tatctgctcg agcgcggcgc ggtcgaagct gccgaacaac tggcgccccg
       61 ctgcgtgcaa tacagcaaga attgcttcgt cgcgatccgg catgaccgcg ggcacgaaca
      121 cgctgcggcc gtccgattcg acgaacggcc ggcctttgtc cggcgaccac gtgctttcct
      181 tcaattcgaa cgggcgctcg aaccagacgt tgagcagcag tgtcagcagc cggttgcgct
      241 cgttcatgcg gaaacccggc aggtgatcga gcaacaccga caggctttct tcgctttcga
      301 gccggaagta cgcttcgccg cgcgcgcggc ccgattgcat gatgtcagcg ccgcgcaacg
      361 cccacggcag caccgcctgc gcgccgagca ggttgcgcac gcgaatcgca ccgccgagat
      421 aagtcgcgag catcagcttg cgcgtctcga aaagctcgtc catcggctcg cacagctgct
      481 cgatacccgt gatgccctgg cgacacgcga gatcgagcac cggcatcgcg aaataagcgc
      541 gcccgctgtc gctgtgccgc gcgtaccaca tgaagccgat ttccgcccag cgccgctcgc
      601 cggcgtggcc cagcgtgccg aacgcgcgcg gcagattctt catgtagcct tcgacggcgg
      661 ccgccggccc cggccaatgc agaaattcca gcgcctgctg cgtccacggc agcaccgttt
      721 cggagatttt ttcggcctcg cggctgccgc gcacgaacgc cttgccggct tcgcgatcgt
      781 gctgaaacag ccgcttgacc gcgtcgagcc agacgagcgc gacgtcgtcg ccatggtgcc
      841 gcaatgccgg cagcgcgtcg gcgacttcgc gatgggcgac aaaggagagc gcgcttaatt
      901 cgaggagcgc gtgctcgata gcggctggcg atgaaacggg catgatggct ggtgatgcag
      961 cggcggccgc gcgagcggcc gcgaggttca ggcgaagtgc gctttgacga tttccatcag
     1021 cgcatcggcg agctcgatgt cgtcggtgag cggattgacc atcgccacct ggcacgcatt
     1081 gaccgggtcg agccccgcgt tgatcatgtt ggccgcgtac accagcgcgc gcgtcgacgt
     1141 cgcctcttca agaccgtggt ctttcagcag ccggcccttg cgcccggtcg cgacgagctt
     1201 cgcggcgagt tcggcatcga tgcccgacac ctcgttgacc acaattttgc gctcgacgtc
     1261 ttccgccgga aaatcgaaat tgatcgcgat gaagcgctgc ttggtcgacg gcttcaggtc
     1321 cttcagcacc gtctgatacc cggggttgta cgagatcacg agcagaaaat cttcgtgcgc
     1381 gtcgatttcc tggcccttct tttcgatcgt gagccggcgc cggtgatcgg tcagcgagtg
     1441 aatcacgacc gtcgaatcgg tgcgcgcctc gacgatttcg tcgaggtagc aaatcgcgcc
     1501 ggccttcacc gcgcgcgtca gcggcccgtc ctgccacacc gtcgagctgc cttcgagcag
     1561 gaagcggccg acgagatcgg agctcgtcag gtcttcgtgg catgacacgg tcaccagcgg
     1621 acgcttgagc gtaaacgcca tgtgctcgag gaaccgcgtc ttgccgcagc ccgtcgggcc
     1681 tttcaacatg accggcaggc gcttgcggta ggcggcctcg aaaattgcca cttcattgcc
     1741 ttgcggctcg tagtacggcg cgatatcgct ggctctgggc tgctggatga tgtcgtgttc
     1801 cacgccgcgc aggcgggttg ctgctgtctt cattcaataa tccttggctg atagctcaat
     1861 actggtcata ttctatcagc aggacgaagg aatcgacagg cgatgggcgg ggtgaagcaa
     1921 aaagcgttag gcgtgaggcg ttaggcgggg ggcctcaatc gcttttcccc tcacgcctca
     1981 cgcctaacgc ctcacaaaat aacgggcccg tccggcagtt
//
LOCUS       DASRDG010000680         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1491107, whole genome shotgun sequence.
ACCESSION   DASRDG010000680 DASRDG010000000
VERSION     DASRDG010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491107"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1074
                     /locus_tag="VFA35_11440"
     CDS             223..1074
                     /locus_tag="VFA35_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZT56833.1"
                     /translation="MAMSDLAAPALPQLTQPLRDLAAWTAHFRAAEIPVLGQTAETLE
                     ALRANEDRVDANSLGEMIAGDPLMTLKVLAHASMQRSERVVTDVETVTAALVMMGISP
                     FFRAFGPQPTVADRLADETEALQGLTDTLRRAHRAANFALGFAVHRMDHDAAVIHAAA
                     LLHDFAEMLLWCHAPALALRIRAMQRADPALRSSAAQRAVLGIELVDLQQALMKAWRL
                     PALLIRISDDRHADSAGVQSVMLALRLARHTAQGWDNAALPEDLTDIATLLNLSPAAT
                     LQFVREI"
     gene            1264..1953
                     /gene="aqpZ"
                     /locus_tag="VFA35_11445"
     CDS             1264..1953
                     /gene="aqpZ"
                     /locus_tag="VFA35_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HZT56834.1"
                     /translation="MMKAYVAEFFGTFWLVLGGCGSAVLAAAFPGLGIGFVGVALAFG
                     LTVVTMAFAIGHVSGCHLNPAVSIGLWAGGRFDAGKLLPYIVSQVLGALVAGGVLYLI
                     ASGKADFSTAGGFASNGYGEHSPGKYSVVAAALCEVVMTAMFLIVIMGSTDKRAPAGF
                     APLAIGLCLTLIHLISIPVTNTSVNPARSTGVAVFAGGWAIEQLWLFWIAPIVGAMLG
                     AFAYRSFGDEH"
     gene            complement(2005..2487)
                     /locus_tag="VFA35_11450"
     CDS             complement(2005..2487)
                     /locus_tag="VFA35_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZT56835.1"
                     /translation="MGNATELDAIDRRILRALQADGRMIYETLAAQVSLSPSATLRRV
                     KRLEESGVIAGYVAIVSPERVGLGLTAYINVRLEKHSEVHKRNPMDLFRAAVQTWPEV
                     VECAALTGEMDYLLRVVVQDMSHYSRFIMDTLLKHPSVEDCKTSFVLDRVKNTTAIPV
                     "
BASE COUNT          405 a          923 c          896 g          426 t
ORIGIN      
        1 cacgcggtag ccgatctcga tcgcaagacg caccgcgtcg atttcggcgg cgcgcgtgcg
       61 ggccgactcg cccatcttcc aggtgcccaa gccgatcgac ggcagcgtgc ttccgtcggg
      121 caacgcgaca gtgttcatgg cggctcccgc aaacgcgatc gtcgatgcta gaccacacgc
      181 gccgagctca agcgcagcac ccaccgcgcc gatatggagt ccatggcgat gtcagacctc
      241 gcggcgcccg cgctgcccca gctcacgcag ccgctgcgcg acctcgccgc gtggacggcg
      301 cacttccgtg ccgccgagat cccggtgctc gggcagaccg ccgagaccct cgaagcgctg
      361 cgcgcgaacg aagaccgcgt cgacgcgaac agcctcggcg agatgatcgc gggcgacccg
      421 ctgatgacgc tgaaggtgtt ggcccacgcg tcgatgcagc gcagcgagcg cgtcgtcacc
      481 gatgtcgaga ccgtcaccgc ggcgctggtg atgatgggca tctcgccgtt cttcagggcc
      541 ttcggcccgc agccgaccgt cgccgaccgg ctcgccgacg agaccgaggc gctgcagggc
      601 ctgaccgaca ccctcaggcg cgcccaccgc gccgcgaact tcgcgctcgg cttcgcggtg
      661 caccggatgg accacgacgc cgcggtgatc cacgcggccg cgctgctgca cgatttcgcc
      721 gagatgctgc tgtggtgcca cgcgccggcg ctggcgctgc gcatccgcgc gatgcagcgc
      781 gccgacccgg cgctgcgctc cagcgccgcg cagcgcgcgg tactcggcat tgaactggtc
      841 gacctgcaac aggcgctgat gaaggcctgg cgcctgcccg cgctgctgat ccgcatcagc
      901 gacgaccgcc atgccgacag cgccggcgtg caaagcgtga tgctggcgct gcgcctggcg
      961 cgacacaccg cgcagggctg ggacaacgcg gccctgcccg aggacctgac cgacatcgcc
     1021 acgctactga acctgtcgcc agcggccacg ctgcaattcg tgcgcgaaat ctgaggcgtc
     1081 gcctggcact ggcgcacgca ctgcgcgcgc agtgtcgcct gcgcgcgccg cgcgggggaa
     1141 acatctcact cgacgcgcac gaagccgcag cgcgcgcgct ttcgcgatgg cagagtgcgc
     1201 gcttttcggc gcgctggcat tcgccaccgc ggcggacccc agggcaacac gacgaggagt
     1261 gcggtgatga aggcatacgt ggcggagttt ttcgggacct tctggctggt gctcgggggc
     1321 tgcggcagcg ccgtgctggc cgcggccttt ccaggcctgg gcatcggctt cgtcggtgtc
     1381 gcgctggcct tcggcctgac ggtggtgacg atggcattcg cgatcggcca tgtgtcgggc
     1441 tgccacctga acccggcggt gtcgatcggc ctgtgggccg gcgggcgctt cgacgcgggc
     1501 aagctgctgc cgtacatcgt ctcgcaggtg ctcggggcgc tcgtcgccgg cggcgtgctc
     1561 tacctgatcg ccagcggcaa ggccgatttc agcaccgccg gcggcttcgc atcgaacggc
     1621 tacggcgagc actcgcccgg caagtactcg gtggtggcgg cggcgctgtg cgaggtggtg
     1681 atgaccgcga tgttcctgat cgtcatcatg ggatcgaccg acaagcgcgc gccggccggc
     1741 tttgcgccgc tggcgatcgg cctgtgcctg acgctgatcc acctgatcag catcccggtg
     1801 accaacacct cggtgaaccc ggcgcgcagc accggcgtcg cagtgttcgc cggcggctgg
     1861 gcgatcgagc agttgtggtt gttctggatc gcgccgatcg tcggggcgat gctgggcgcg
     1921 ttcgcgtacc gctcgttcgg cgacgagcac tgagcccgcg ctcgcgccga ctcgcgcacg
     1981 gcgctgcggc gccctgcttc gctgctagac cggaatcgcg gtcgtgttct tcacgcggtc
     2041 gagcacgaag ctggtcttgc agtcctcgac gctcgggtgc ttcagcagcg tgtccatgat
     2101 gaagcgcgag tagtgcgaca tgtcctgcac gacgacgcgc agcaggtagt ccatctcgcc
     2161 ggtcaacgcg gcgcactcga cgacctcggg ccaggtctgc accgcggcgc ggaacaggtc
     2221 catcgggttg cgcttgtgca cctcggagtg cttctcgagc cgcacgttga tgtaggccgt
     2281 caggcccagg ccgacgcgct cgggcgagac gatcgcgaca tagccggcga tcacgcccga
     2341 ttcctccagc cgcttgaccc gccgcagggt cgccgacggc gacaggctga cctgcgcggc
     2401 gagcgtttcg taaatcatcc ggccgtcggc ctgcaacgcg cgaagaatgc gccgatcgat
     2461 ggcatccagc tctgttgcgt ttcccatctc gatgattttg cagctttgca gcgctcaatt
     2521 caagttgaaa gcagcaactt gcagaaaaac tgcgggtcaa cgaacctaca gtttcatggc
     2581 gacgacgtga cgcacgttca acccttgccg aggagcgcca aatgcaattc accccctggg
     2641 acaacccgat
//
LOCUS       DASRDG010000681         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_562445, whole genome shotgun sequence.
ACCESSION   DASRDG010000681 DASRDG010000000
VERSION     DASRDG010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562445"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VFA35_11455"
     CDS             <1..830
                     /locus_tag="VFA35_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZT56836.1"
                     /translation="KRHLSFALTEPGHGSDATWLETTGRRDGDHWVIDGMKRWNSQVA
                     RAHGNLVFARTSGKPGDGRGITAFIVPTDTPGHNILYNHWTFNMPSDHAEVELKGVRV
                     PDSAILHKEGEGLMVAQRFVHENRIRQAAASAGAARYCITEAAKYARSRIAFGEPLSK
                     KQAVQFPLVELYAECEMVRNFIFKTAWQMDRQDPLEISDWVSICNFRANRLCCEAADR
                     AMQVHGGIGYSRALPFEHIYRHHRRYRITEGSEEVQMRRVAQYLFGFSGKMATSGAQ"
     gene            830..1888
                     /locus_tag="VFA35_11460"
     CDS             830..1888
                     /locus_tag="VFA35_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZT56837.1"
                     /translation="MGGSTVPAPERDLDFDAGRLEAYLRALLGPADGPLEARRTEGGM
                     SNPTYFLRRGGWQAVLRKQPGGVVLPSAHAIDREYRVLSALRDTDVPVPRPLHYCADR
                     EVLGTPFYLMEKLDGRVFHEFSTPGLSRGDRAALFDSMVGTMAAIHRLDVAAIGLADY
                     GRPGNYFARQLKRWSEQWVTFRRGDDDNPALDRIVAWLSERVPESELQTLCHGDFRIG
                     NMMFHGTEPRVIGVLDWELSTLGHPLVDVAFNSQAWRMAPDENGGLLGLPLAELGIPD
                     EETYLDRYYALARSGERMTTFHQVFAMFRGAVGSAGVAARGEGGNAFLPDAARVGRKL
                     ALAYAARGLALIRERGEE"
     gene            1896..>2494
                     /locus_tag="VFA35_11465"
     CDS             1896..>2494
                     /locus_tag="VFA35_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZT56838.1"
                     /translation="MKEIAIVGPGLMGLGIAQVAAAAGWHVRLVGRDAAAAVAGRVRL
                     ATQLDRQVARGRLDRTVADALLDRVGAVADDAELGRCELAIESVPEDRALKLAVLSRL
                     QDALPAGTLIATNTSGLPVSGLAAALRRPERFVGLHFFSPVERMKLVEVVRGKATAQA
                     TLREALAFVEDLGQTPIVVRDGPGFFTSRVFAAYLDEALA"
BASE COUNT          365 a          857 c          882 g          390 t
ORIGIN      
        1 gcaagcggca cctctcgttc gcgctcaccg agcccggcca cggcagcgac gcgacctggc
       61 tcgagacgac cgggcgccgc gacggcgatc actgggttat cgacggcatg aagcgctgga
      121 acagccaggt cgcgcgcgcg cacggcaacc tcgtgttcgc gcgcacttcg ggcaagccgg
      181 gcgatggccg cggcatcacc gccttcatcg tgccgaccga cacgccgggt cataacatcc
      241 tctacaacca ctggaccttc aacatgccga gcgaccatgc ggaggtcgag ctgaagggcg
      301 tacgcgttcc cgacagcgcg atcctgcaca aggaaggcga ggggctgatg gtggcgcagc
      361 gcttcgtcca cgagaaccgg atccgccagg cggcggcaag cgccggcgcg gcgcgctact
      421 gcatcaccga ggcggcgaag tacgcgcgct cgcgaatcgc gttcggcgag cccttgtcga
      481 agaagcaggc agtccagttt ccgctcgtcg agctctatgc cgaatgcgag atggtgcgca
      541 acttcatctt caagaccgcc tggcagatgg accggcagga cccgctcgag atcagcgact
      601 gggtctcgat ctgcaacttc cgcgcgaacc gcctctgctg cgaggcggcc gaccgggcga
      661 tgcaggtgca cggcggcatc ggctacagcc gtgccctgcc gttcgagcac atctaccggc
      721 accatcgccg ctaccgcatc accgagggct cggaggaagt gcagatgcgg cgcgtcgccc
      781 agtacctgtt cggcttcagc ggcaagatgg cgacatccgg cgcccagtga tgggcgggtc
      841 gactgtcccg gcgccggagc gcgacctcga cttcgatgcg gggcgcctgg aggcgtatct
      901 ccgtgcgctg ctcggtccgg cggacggccc gttggaggct cggcgaaccg agggtgggat
      961 gtcgaacccg acctacttcc tgcgccgggg agggtggcag gcggtcctgc gcaagcagcc
     1021 gggcggggtg gtgctgccat cggcgcacgc cattgaccgc gagtaccgcg tgctgagcgc
     1081 gttgcgcgac accgacgtgc cggtgccgcg gcccttgcac tactgcgccg accgcgaagt
     1141 gctcggcacc ccgttctacc tgatggaaaa gctcgacggc cgcgtgttcc acgagttctc
     1201 gacaccgggc ctgtcgcgcg gcgaccgggc ggcgttgttc gattcgatgg tcggcacgat
     1261 ggccgcgatc caccgcctcg acgtcgcggc gatcggcctg gccgactacg gtcgtccggg
     1321 caactacttc gcgcgccagc tgaagcgctg gtccgagcaa tgggtgacgt tccgacgcgg
     1381 cgacgacgac aaccccgcgc tcgatcgcat cgtcgcctgg ctgtccgagc gcgtgcccga
     1441 gagcgagctg cagacgcttt gccacggtga tttccgcatc ggcaacatga tgttccacgg
     1501 caccgagccg cgagtgatcg gcgtcctcga ctgggaactc tcgacgctcg gccatccgct
     1561 cgtcgatgtc gccttcaaca gccaggcctg gcggatggcg cccgacgaga acggcggctt
     1621 gctcggcctt ccgctggccg agctcggcat ccccgacgag gagacctacc tcgatcgcta
     1681 ctacgcgctc gctcgatctg gcgagcgcat gacgaccttc caccaggtgt tcgcgatgtt
     1741 tcgcggcgcc gtcggcagcg ccggcgtggc ggcgcgcggg gagggcggca atgcctttct
     1801 gcccgacgcg gcgcgcgtcg gtcgcaagct cgcgctggcg tacgcggcgc gcggcctggc
     1861 cctgatccgc gagcgcggcg aggaatgacc gggcgatgaa ggagatcgcg atcgtcggcc
     1921 cgggtctgat ggggcttggc atcgcccagg tcgctgccgc ggccggttgg catgtgcggc
     1981 tggtgggtcg cgacgccgcg gccgcagtgg ccgggcgcgt gcggctcgcg acacagctcg
     2041 accggcaggt cgcgcgcggt cgcctcgatc gcaccgtggc cgatgccttg ctcgaccggg
     2101 tcggcgcggt cgccgacgac gccgagctcg gccgctgcga gctcgccatc gagtcggtgc
     2161 cggaggatcg tgccctcaag ctggccgtgt tatctcgtct gcaggatgcg ctgccggcgg
     2221 gcacgctgat cgcgaccaac acctcgggtt tgccggtgag cgggctggcc gcggcgctgc
     2281 gccggcccga gcgcttcgtc ggcctgcact tcttctcgcc cgtggagcgg atgaaactgg
     2341 tcgaggtggt gcgcggaaag gcgaccgcgc aggcgacgct gcgcgaagcc cttgcgttcg
     2401 tggaagacct gggccagacc ccgatcgtcg tgcgtgacgg ccccggattc ttcacctcgc
     2461 gcgtgttcgc tgcctatctc gacgaagccc tggc
//
LOCUS       DASRDG010000682         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_679877, whole genome shotgun sequence.
ACCESSION   DASRDG010000682 DASRDG010000000
VERSION     DASRDG010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679877"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFA35_11470"
     CDS             complement(<1..330)
                     /locus_tag="VFA35_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56839.1"
                     /translation="MTAPTLPPEAESWLHIGPGTYYAPDIALKVWLPSERVVIGNYCS
                     IAERVSIFTGGMRRTDTAALYPFNVQQAYRSTADTTIGHDVWIGYGATIFRGARIGNG
                     AIVGAGAV"
     gene            complement(379..612)
                     /locus_tag="VFA35_11475"
     CDS             complement(379..612)
                     /locus_tag="VFA35_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HZT56840.1"
                     /translation="MQPDDDDTERYRVVINQEEQYSIWPADMDNAPGWSDAGAAGSKQ
                     ACLDYIREHWTDMRPASLRRAMEAAGDDASAIP"
     gene            complement(635..1432)
                     /locus_tag="VFA35_11480"
     CDS             complement(635..1432)
                     /locus_tag="VFA35_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZT56841.1"
                     /translation="MSGGERPCPSAPPGDGWLQCLRPAAMPRLRLIALPHAGGGPWAY
                     RSWRDHLAPPIELWAVQLPGRGSLLDQPARTALPPLVDELRQAVAPHLNLPYALFGHS
                     MGALLAFELARALRRHARPPPLALFVAGHRAPQLPRTEPDLHHLSDRALAAEVAQFDG
                     TPDELLDDAALSALFVPILRADFTVCETYRYQHEDPLLAPIFALGADDDPLVPRPQLE
                     AWRVQTVGGFELMMVPGNHFFIRGSAAVICTRLNSELLARTTAQAPA"
     gene            complement(1429..>2758)
                     /locus_tag="VFA35_11485"
     CDS             complement(1429..>2758)
                     /locus_tag="VFA35_11485"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZT56842.1"
                     /translation="TLDALCDVLLGELADGAAPPVVACRPMRCVPAFAQVRINPAGPR
                     MQRVRAGGVYLITGGLGGIGLVLAQYLAAQAPVKLVLLGRSVLPARAAWPGLLARDPD
                     APLARKVRAIQALEGAGADVMLLDADVADRTRMEQVAREVRAGFGSVNGIVHAAGVAG
                     GGLIALKTPEDAARVMAPKTQGMRVLAAVFADAPLDFVVLCSSLSAQLGGVGQIDYCA
                     ANCYLDAFAWQNPWRARGVAVISIDWDTWGEVGMAVETRVPEDLRAQQQEALRRGIRN
                     AEGVAAFDIVISDNQPQVALSTVPLALRQAAAVRARAGAAGAQPAPQARAHPRPALST
                     EHVAPRTNTERQVAAIWSQVLGIADVGVDDNFLELGGHSLLAVQLLSRLRDALLAEVP
                     LQTFFAAPTVAAVSTLIDAGASAADPFEAPPLVPLPRERYRQHGEGGSLP"
BASE COUNT          384 a          969 c         1020 g          385 t
ORIGIN      
        1 caccgcgccg gccccgacga tggcgccgtt gccgatccgg gcgccgcgga agatggttgc
       61 gccgtagccg atccagacgt catggccgat cgtcgtgtcg gccgtgctgc ggtaggcctg
      121 ctggacgttg aagggataga gcgccgccgt gtcggtgcgg cgcatgccgc cggtgaagat
      181 gctgactcgc tcggcgatcg agcagtagtt gccgatgaca acccgttcgc tgggtagcca
      241 gactttcagc gcgatgtcgg gcgcatagta agtgccgggg ccgatgtgca gccaggactc
      301 ggcctcgggg ggtagcgtgg gtgcggtcat gcgctctcct tggcacgcga tgggcggccg
      361 cagcggcggc gggccgggtc aagggatggc gcttgcatcg tcgccggccg cttccatggc
      421 gcggcgcagg ctcgccggac gcatgtcggt ccagtgctcg cggatgtagt ccaggcaggc
      481 ctgcttgctg ccggcggcgc cggcgtccga ccagccgggg gcgttgtcca tgtcggccgg
      541 ccagatggaa tattgctctt cctggttgat caccactcgg tagcgctcgg tgtcgtcgtc
      601 gtcgggctgc atcgcggtct ccttcgaatc cgggtcaagc cggcgcctgc gccgtggtac
      661 gcgccagcag ttcgctgttc aggcgggtgc aaatcacggc ggcgctgccg cggatgaaga
      721 aatggttgcc gggcaccatc atgagctcga agccgccgac ggtctgtacg cgccaagcct
      781 ccagctgagg acgcgggacg agcgggtcgt cgtcggcgcc gagcgcgaag atcggcgcca
      841 gcagcgggtc ctcgtgctgg tagcggtagg tttcgcagac cgtgaagtcg gcgcgcagga
      901 tgggcacgaa cagcgcactc agggccgcgt cgtccagcag ctcgtcgggc gtgccgtcga
      961 actgggcgac ctcggcggcg agggcgcggt cgctcaggtg gtgcaggtcg ggctcggtgc
     1021 gcggcagctg cggcgcgcgg tgccccgcca cgaagagcgc cagcggcggc ggccgggcgt
     1081 gccgtcgcag tgcgcgcgcc agttcgaagg cgagcagtgc gcccatgctg tggccgaaca
     1141 gcgcgtacgg caggttgagg tgcggcgcga ccgcctgccg tagctcgtcc accagcggcg
     1201 gcagcgcggt gcgtgccggc tggtcgagca ggctgccgcg gcccggcaac tgcacggccc
     1261 agagttcgat cggcggtgcc aggtggtcgc gccagctgcg gtaggcccaa ggtccgcctc
     1321 ccgcatgcgg cagggcgatc aggcgcagcc gtggcatcgc cgccggccgc aggcactgca
     1381 gccagccgtc gcctggcggc gccgatgggc acgggcgctc gccgccgctc atggcaggct
     1441 gccaccctcg ccgtgctgcc ggtagcgctc gcgcggcagc ggcaccaatg gcggtgcctc
     1501 gaacggatcg gctgccgacg cgccggcgtc gatcagtgtg ctcaccgccg ccacggtcgg
     1561 cgcggcgaag aaggtctgca gcggcacctc ggccagcagc gcatcgcgta gccgcgacaa
     1621 cagctgcacc gccagcagcg agtggccgcc gagctcgagg aagttgtcgt cgacgccgac
     1681 gtcggcgatg cccagcacct gcgaccagat ggcggccact tggcgctcag tgttggtacg
     1741 tggcgccacg tgctcggtcg acagggccgg gcggggatgc gcacgcgcct gcggcgcagg
     1801 ctgcgccccg gccgcgccgg cgcgagcgcg cactgctgcg gcctggcgca gtgccagagg
     1861 caccgtcgac agcgccacct gcggctggtt gtcggagatg acgatgtcga aggcggccac
     1921 gccctcggcg ttgcggatgc cgcgccgcag cgcttcctgt tgctgcgccc gcaggtcttc
     1981 gggcacgcgg gtctcgaccg ccattccgac ctcgccccag gtgtcccagt cgatcgagat
     2041 caccgccacg ccgcgcgcgc gccacgggtt ctgccacgcg aaggcgtcga ggtagcagtt
     2101 ggcggcgcag tagtcgatct ggccgacacc gcccagttgc gccgacagcg acgaacacag
     2161 cacgacgaag tcgagtggcg cgtcggcgaa caccgctgcc agcacgcgca tgccttgggt
     2221 cttgggcgcc atcacgcgcg ccgcgtcctc aggcgtcttc agggcgatca ggccgccgcc
     2281 ggcgacaccg gcggcgtgca cgatgccgtt gaccgacccg aagccggcac gcacctcccg
     2341 tgccacctgc tccatgcgcg tgcgatcggc gacatcggcg tcgagcagca tcacgtccgc
     2401 accggcgcct tccagcgcct ggatcgcccg gaccttgcgc gccagcggag catcgggatc
     2461 gcgcgccagc aggcccggcc aggcagctcg tgctggcagc acgctgcggc ccagcagcac
     2521 cagcttcacc ggtgcctgcg cggccaggta ctgcgccagc accagcccga tgccacccag
     2581 gccgccggta atcaggtaca cgccgcccgc ccgcacgcgc tgcatgcgcg gaccggccgg
     2641 gttgatgcgc acctgcgcaa acgctggcac gcagcgcatt ggccggcagg ccaccacagg
     2701 cggcgctgcg ccgtcggcga gttcgcccag caggacatcg cacaaggcgt ccagcgtc
//
LOCUS       DASRDG010000683         5112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1491791, whole genome shotgun sequence.
ACCESSION   DASRDG010000683 DASRDG010000000
VERSION     DASRDG010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491791"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFA35_11490"
     CDS             complement(<1..641)
                     /locus_tag="VFA35_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HZT56843.1"
                     /translation="MKRRSTLKSLIAVAASLAGATARAQTDGDPFGSFQWPDLKREFL
                     HGGPVRFDPRVQVRGPAFAEDPMNVPITVSADGLRDVDVIVVIVDRNPIRKVLEYFPL
                     GALPAVSFRFKLEQASPVRAAVRTRDGAWHVGGVFVDSAGGGCTAPGATRRDGSWSRT
                     LGEVSARVFPHSVPLGGGGAGTRLRLRVMHPMDTGLVGGVPAFYLNRLALVDA"
     gene            903..2429
                     /locus_tag="VFA35_11495"
     CDS             903..2429
                     /locus_tag="VFA35_11495"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZT56844.1"
                     /translation="MDMFELKTVGIDTEYPYKKQYGNYIGGQWLAPVSGEYFDNVSPL
                     NGKPFCSIPRSKAGDIEIALDAAHKARRGWAQTSVTDRANVLLKIADRMEQNLKLLAV
                     AETIDNGKPLRETMAADVPLAIDHFRYFAGCIRAQEGAISEIDKDTVAYHFDEPLGVV
                     GQIIPWNFPLLMAAWKLAPAMAAGCCIVMKPAEQTPASILVLMELVGDLIPPGVVNIV
                     NGFGREAGEALATSKRIAKIAFTGSTPVGKRILQAAADNLIPSTVELGGKSPNIFFED
                     VMDADDAFFNKTLEGLAMFALNQGEVCTCPSRIFIQESIYEKFIAKAIARVEAIKTGN
                     PLDATTMVGAQASQQQLDKILGYIEIGRGEGATCLTGGARNLIGGGLDAGYYIQPTML
                     LGHNKMRVFQEEIFGPVASVLTFKDEAEAIALANDTSFGLGAGVWSRDGARAYRMGRE
                     IEAGRVWTNCYHLYPAHAAFGGYKQSGIGRETHKMALGNYQHTKNLLVSYNPNAMGFF
                     "
     gene            2507..3553
                     /locus_tag="VFA35_11500"
     CDS             2507..3553
                     /locus_tag="VFA35_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZT56845.1"
                     /translation="MRIETIRQRLREHGAGPLHEHRVLRLWATAQSQASGKRRPEDFL
                     PQTVRAMLPALVDELAALARLRSEHPADDGSSRLLVELADGQAVESVLLPRGGLCVST
                     QLGCAVGCVFCMTGREGLLRQLGSAEIVAQVVLARTLRAVTKVVFMGMGEPAHNLDNV
                     TEAIELLGSAGGIGHKNLVFSTVGDVRVFEQLPRLRVRPALALSLHSTRPALRAQLMP
                     RAPRITPAELVELGERYARDTGHPVQYQWTLLAGINDTDEELDGIVELLRGKYALLNM
                     IPYNTVDGLAFKRPSRERAEAIARSLHARGVLTKLRQSAGQDVDGGCGQLRARALAPA
                     AQAVTVHRRAQVLA"
     gene            3550..4143
                     /locus_tag="VFA35_11505"
     CDS             3550..4143
                     /locus_tag="VFA35_11505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZT56846.1"
                     /translation="MNQPPLTPDKEQIATLEPFERLPLSRIELVATGAQAERAAAELA
                     GATVLGFDTESKPTFAKGEVSDGPHIVQLATLAKAWIFQLHDPACRAALCELLASNAA
                     QKVGFGLGDDMRRIRAKLGVEALGVVDLNHLFRAQGYRKDIGVKTAVALLFGKRFLKS
                     GKASTSNWAARQLSESQLMYAANDAWAAARAYEAMHR"
     gene            4196..4540
                     /locus_tag="VFA35_11510"
     CDS             4196..4540
                     /locus_tag="VFA35_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56847.1"
                     /translation="MKDFPAREKLDLTEKVARYLVLAGTLDKNSAPDDYDMANELSLE
                     LAMVLPTPIYRAMVEAAAHPDGKVNPATVVVMMRKELLADEPNASDPELHPEQVVFHT
                     PGVATKPRSKAH"
     gene            4562..4831
                     /locus_tag="VFA35_11515"
     CDS             4562..4831
                     /locus_tag="VFA35_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HZT56848.1"
                     /translation="MCRSIKTLHNFEPPVTAEEIHAASLQFVRKLSGFHKPSKANEAA
                     FEQAVTEIAQVAGTLLASLETHAPPRDRDEEAAKARARAAVRFAA"
     gene            complement(4841..>5112)
                     /locus_tag="VFA35_11520"
     CDS             complement(4841..>5112)
                     /locus_tag="VFA35_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HZT56849.1"
                     /translation="VEQLLRGVVLHDDPLPVRAAACEQTGEHALRMTLLEGKYHQVKR
                     MVAATGNTVNSLHRSAYGVLALPADLAPGQWRWLAGPAAVLGAGG"
BASE COUNT          848 a         1814 c         1735 g          715 t
ORIGIN      
        1 tcagcgtcca cgagcgcgag ccggttcagg tagaacgccg ggaccccgcc gaccaggccg
       61 gtgtccatcg gatgcatcac gcgcaggcgc aggcgcgtgc cggccccgcc gccaccgagc
      121 ggcacgctgt gcgggaacac gcgcgcgctg acctcgccga gcgtgcgcga ccagctgccg
      181 tcgcgccgcg tcgcgcccgg cgcggtgcag ccgccgcccg ccgagtcgac gaacacgccg
      241 ccgacgtgcc aagcgccgtc gcgcgtgcgc accgccgcgc gcaccgggct cgcctgctcg
      301 agcttgaagc gaaagctcac cgcgggcagc gcgccgagcg gaaagtactc gagcaccttg
      361 cggatcgggt tgcggtcgac gatcaccacg atcacgtcga catcgcgcag gccgtcggcc
      421 gagaccgtga tcggcacgtt catcgggtct tcggcgaacg ccgggccgcg gacctgcacg
      481 cgcgggtcga accgcaccgg cccgccgtgc aggaactcgc gcttcaaatc cggccactgg
      541 aacgagccga acgggtcgcc gtcggtctgc gcgcgcgcgg tcgcgccggc cagcgaggct
      601 gcgaccgcga tcaacgattt caatgtcgaa cgacgcttca tggatgtgtg cccccgcgca
      661 tccgaagcaa cttctgtgct gcggcgggca gtggctgcgc tgttcctttg cgtgacaagc
      721 aggctggcgg tgtcagcgtt aacacgtagc cggcgcgccg catttgttca gaaccgcagc
      781 agcttcgcga cctcggaggc ctggcacgcg gcgtgcgcat cgatcggttg cggctcggca
      841 gttcgctcga gcgagcgaca ccccgagcgg tttcattttt tcttttcaac cggagacaag
      901 ccatggacat gttcgaactg aagacggtgg ggatcgacac cgaatacccg tacaagaagc
      961 agtacggcaa ctacatcggc ggccagtggc tggcgccggt gtcgggcgag tacttcgaca
     1021 acgtctcgcc gctcaacggc aagccgttct gcagcatccc gcgctccaag gccggtgaca
     1081 tcgagatcgc gctcgacgcc gcccacaagg cgcgccgcgg ctgggcccag acctcggtga
     1141 ccgaccgcgc gaacgtgctg ctgaagatcg ccgaccgcat ggagcagaac ctgaagctgc
     1201 tggcggtggc cgagaccatc gacaacggca agcccctgcg cgaaacgatg gctgccgacg
     1261 tgccgctggc catcgaccac ttccgctact tcgccggctg catccgcgcc caggaaggcg
     1321 cgatctccga gatcgacaag gacacggtgg cctaccactt cgacgagccg ctcggcgtcg
     1381 tcggccagat catcccgtgg aactttccgc tactgatggc ggcgtggaag ctggcgcccg
     1441 cgatggccgc cggctgctgc atcgtgatga agccggccga gcagacgccg gcctcgatcc
     1501 tggtgctgat ggaactggtc ggcgacctga tcccgcccgg cgtcgtcaac atcgtcaacg
     1561 gcttcggccg ggaagccggc gaggcgctgg ccaccagcaa gcgcatcgcc aagatcgcct
     1621 tcaccggctc gacgccggtg ggcaagcgca tcctgcaggc cgccgccgac aacctgatcc
     1681 cgtcgaccgt cgagctcggc ggcaagagcc ccaacatctt cttcgaagac gtgatggatg
     1741 ccgacgacgc gttcttcaac aagacgctcg aaggcctggc gatgttcgcg ctgaaccagg
     1801 gcgaggtctg cacctgcccg tcgcgcatct tcatccagga atcgatctac gagaagttca
     1861 tcgcgaaggc gatcgcgcgc gtcgaggcca tcaagaccgg caacccgctg gacgccacca
     1921 cgatggtcgg cgcgcaggcc tcgcagcagc agctcgacaa gatcctgggc tacatcgaga
     1981 tcggccgcgg cgaaggcgcc acctgcctga ccggcggcgc gcgcaacctg atcggcggtg
     2041 gcctggatgc cggctactac atccagccga cgatgctgct gggccacaac aagatgcgcg
     2101 tgttccagga ggagatcttc ggcccggtcg cgtcggtgct gaccttcaag gacgaggccg
     2161 aagccatcgc gctcgccaac gacaccagct tcgggctcgg cgccggcgtg tggtcgcgcg
     2221 acggcgcgcg cgcctatcgc atgggccgcg agatcgaggc cggccgggtc tggaccaact
     2281 gctaccacct ctacccggcg catgccgcgt tcggcggcta caagcagtcg ggcatcggcc
     2341 gcgagacgca caagatggcg ctcgggaact accagcacac gaagaacctg ctggtcagct
     2401 acaacccgaa cgcgatgggc ttcttctgat cgccccaggg ccgggctgcg cccggcccgc
     2461 cccgctcccc gcggggattc gcgcgagcgc ctaaactcgc gcgctcatgc gcatcgaaac
     2521 catccgccag cggctgcgcg agcacggcgc cggcccgctg cacgaacacc gcgtcctgcg
     2581 cctgtgggcg acggcgcagt cgcaggccag cggcaagcgc cggccggaag actttctgcc
     2641 gcaaaccgtg cgcgcgatgc tgcccgcgct cgtcgacgag ctcgccgcgc tggcccggct
     2701 gcgctccgaa cacccggccg acgacggttc gtcgcggctg ctggtcgagc ttgccgacgg
     2761 ccaggcggtc gagagcgtgc tgctgccgcg cggcggtttg tgcgtgtcga cgcaactggg
     2821 ctgcgcggtc ggctgcgtgt tctgcatgac cgggcgcgag ggcctgctgc gccagctcgg
     2881 cagcgccgag atcgtcgcgc aggtcgtgct cgcgcgcacg ctgcgcgcgg tcacgaaggt
     2941 cgtcttcatg ggcatgggcg agccggcgca caacctcgac aacgtgaccg aggcgatcga
     3001 gctgctcggc agcgccggcg gcatcggcca caagaacctg gtgttctcga ccgtcggcga
     3061 cgtgcgcgtg ttcgaacagc tgccgcgcct gcgggtgagg ccggcgctgg cgctgtcgct
     3121 gcattcgacg cggccggcgt tgcgcgcgca gttgatgccg cgcgcgccgc gcatcacgcc
     3181 ggccgagctg gtcgagctcg gcgaacggta cgcgcgcgac accggccatc ccgtccagta
     3241 ccagtggacc ttgctcgccg gcatcaacga caccgacgaa gagctcgacg gcatcgtcga
     3301 gctgctgcgc ggcaagtacg cgctgctgaa catgattccc tacaacaccg tcgacggcct
     3361 ggccttcaag cgtccctcgc gcgagcgcgc cgaggcgatc gcgcgctcgc tgcatgcgcg
     3421 cggcgtgctg acgaagctgc gccagtcggc cgggcaggac gtcgatggcg gctgcggtca
     3481 gctgcgcgcc cgcgcgctcg cgccggccgc gcaggccgtc acggtccatc gtcgtgcgca
     3541 ggtgctcgca tgaatcaacc gcctttgacc cccgacaagg aacagatcgc cacgctcgaa
     3601 ccgttcgagc gcctgcctct gtcgcgcatc gagctcgtcg cgacgggtgc gcaggccgag
     3661 cgcgcggccg ccgaactggc gggcgcgacg gtgctcggtt tcgacaccga atcgaagccg
     3721 accttcgcga agggcgaggt ctccgacggg ccgcacatcg tgcagctcgc gacgctggcc
     3781 aaggcgtgga tcttccagct ccacgacccg gcctgccgcg ccgcgctgtg cgagctgctc
     3841 gcgtcgaacg ccgcgcagaa ggtcggcttc ggcctcggcg acgacatgcg ccgcatccgc
     3901 gccaaactcg gtgtcgaggc gctcggcgtg gtcgacctga accacctgtt ccgcgcccag
     3961 ggctaccgca aggacatcgg cgtgaagacc gcggtcgcgc tgctgttcgg caagcgcttt
     4021 ctcaagtcgg gcaaggccag cacctcgaac tgggccgcgc gccagctcag cgagagccag
     4081 ctgatgtacg cggcgaacga cgcctgggcc gcggcgcggg cctacgaggc gatgcaccga
     4141 tgacgacgcg gtgctgtacg ctcgaaggct cgaccgcgtt ccaggagaca tggacatgaa
     4201 agacttcccc gcccgcgaaa agctcgacct gaccgagaag gtcgcccgct acctggtgct
     4261 ggccggcacg ctcgacaaga actccgcacc cgacgactac gacatggcga acgaactcag
     4321 cctcgagttg gcgatggtgc tgcccacgcc gatctaccgc gcgatggtcg aggccgcggc
     4381 gcaccccgac ggcaaggtga acccggcgac cgtcgtcgtg atgatgcgca aggaactgct
     4441 cgcggatgag ccgaacgcga gcgaccccga actgcacccc gagcaggtcg tattccacac
     4501 ccccggcgtt gcgaccaagc cgcgcagcaa agcgcactga tcttcaaagg agggcctcgc
     4561 gatgtgccga agcatcaaga cgctgcacaa cttcgagccg ccggtgacgg cggaagagat
     4621 ccacgcggcc tcgctgcagt tcgtgcgcaa gctcagcggc ttccacaagc cgtcgaaggc
     4681 gaatgaggcg gcgttcgagc aggcggtgac cgagatcgcg caggtcgccg gcacgctgct
     4741 ggcctcgctc gaaacccacg cgccgccacg cgatcgcgat gaagaggcgg cgaaggccag
     4801 ggcccgcgcg gcggtgcgtt tcgccgcgta gcgcggcgag tcagccgccg gcgccgagta
     4861 ccgcggccgg cccggcgagc cagcgccatt gccccggcgc cagatcggcc ggcagcgcga
     4921 gcacaccgta ggcgctgcgg tgcagcgaat tgaccgtgtt gccggtcgcg gcgaccatgc
     4981 gcttgacctg gtggtacttg ccttcgagca gggtcatgcg cagcgcgtgt tcgccggtct
     5041 gctcgcacgc cgcggcgcgc accggcagcg ggtcgtcgtg cagcacgacg ccgcgcagca
     5101 gctgctcgac ct
//
LOCUS       DASRDG010000684         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1141481, whole genome shotgun sequence.
ACCESSION   DASRDG010000684 DASRDG010000000
VERSION     DASRDG010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141481"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VFA35_11525"
     CDS             <1..1047
                     /locus_tag="VFA35_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZT56850.1"
                     /translation="FTELQALPDAAPPAGYLWVGIARAEFERNIATLQSALQRWTGGQ
                     LVDLHVSDLLNPQLPSHFDYTSWYDLLVFRRLAATSADDERDAPAAALTRIDTSPVGF
                     AVFDRVLLTVHPADCMVREYFANRLQQQTTGTEARAGGARVPPSPADMALRMVNHMVD
                     SYLDLRRLLTKQLDLLQQEMFGPRPDPGSWKLLLDSRDGLHLLEDTCEDQRAAITEWI
                     DALDELPPEGDTALLRERELLRVRSRDVLEHIERVLTHVRRLESSAENAVQMHFSAQS
                     NRTNEIMRTLTVLTAIFLPLNLVTGFFGMNFDALPGIHSPRAFWITFGTMLVVGVGLS
                     LHFWRKRYIGRVPH"
     gene            complement(1081..1596)
                     /gene="ybeY"
                     /locus_tag="VFA35_11530"
     CDS             complement(1081..1596)
                     /gene="ybeY"
                     /locus_tag="VFA35_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZT56851.1"
                     /translation="MKPLVRRVRRTIPRGDAGRLGSGPALGPPLTLSLQFADASDRAV
                     LPRHRVARWIRAALEAPGEITVRIVGADEGRALNREYRAKDYATNVLTFDYSRAPVVS
                     ADLVLCAPVLQREAHELGIPLAAHYAHMLVHGTLHAQGYDHELDAEARAMEARESELM
                     RALGFEDPYAK"
     gene            complement(1598..2551)
                     /locus_tag="VFA35_11535"
     CDS             complement(1598..2551)
                     /locus_tag="VFA35_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZT56852.1"
                     /translation="MILRHAFEPRDNARLAHLCGSLDEHLRSIERLLDVSVSRRNEFF
                     RIEGPKPAAAHAMNLLLALYEQAQRPIDAEQLQLALVEVGANAALPEAARGDTPTLRT
                     RRAELAGRTPNQVTYLRNILAHDITFGIGPAGTGKTFLAVACAVDALERSAVQRVILT
                     RPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGADKVAKAFEKGTLEIAPLAFM
                     RGRTLNHAFVILDEAQNSTVEQMKMFLTRIGFGTKVVINGDVSQIDLPRGTESGLIDA
                     ERVLRRVRGIATTRFTSSDVVRHPLVARIVEAYDAARLDEA"
     gene            2678..>2983
                     /locus_tag="VFA35_11540"
     CDS             2678..>2983
                     /locus_tag="VFA35_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56853.1"
                     /translation="MPKPPAPLAAIRWLGRRCLAGWLALVGVLAICAPALGADAQIVT
                     IDRAVSVQQMVEPGSVQQLALPVKLPDDWALTRPRFEGSVWYRTGFDRPTGVDSHELM
                     "
BASE COUNT          401 a         1071 c         1055 g          456 t
ORIGIN      
        1 ttcaccgagc tgcaggcgct gcccgacgcg gcgccgcccg ccggttatct gtgggtcggc
       61 atcgcgcgcg ccgagttcga gcgcaacatc gcgaccctgc agagcgccct tcagcgctgg
      121 accggcggcc agctcgtcga cctgcacgtc tccgacctgc tgaacccgca gctgccctcg
      181 catttcgact acacctcgtg gtacgacctg ctggtgtttc gccgactcgc ggcgaccagc
      241 gccgacgacg agcgcgatgc gccggccgcc gcgctgaccc gcatcgacac cagcccggtc
      301 ggcttcgcgg tgttcgaccg cgtgctgctg accgtgcacc cggccgactg catggtgcgc
      361 gaatacttcg cgaaccggct gcagcagcag acgaccggca ccgaggcgcg cgccggcggc
      421 gcgcgcgtgc cgccgagccc ggccgacatg gcgctgcgca tggtcaacca catggtcgac
      481 agctacctcg acctgcgccg gctgctgacc aagcagctcg acctgttgca gcaagagatg
      541 ttcggcccgc ggcccgaccc gggcagctgg aagctgctgc tcgactcgcg cgacggcctg
      601 cacctgctcg aagacacgtg cgaagaccag cgcgccgcga tcaccgagtg gatcgacgcg
      661 ctcgacgagc tgccgcccga gggcgacacc gcgctgctgc gcgagcgcga gctgctgcgg
      721 gtgcgctcgc gcgacgtgct cgagcacatc gagcgcgtgc tcacgcacgt gcggcggctc
      781 gagtcctcgg ccgagaacgc agtgcagatg cacttctcgg cgcagagcaa ccgcaccaac
      841 gagatcatgc gcacgctgac ggtgctgacc gcgatcttcc tgccgctgaa tctcgtgacc
      901 ggcttcttcg gcatgaactt cgacgcgctg cccggcatcc acagcccgcg cgcgttctgg
      961 atcaccttcg gcacgatgct ggtggtcggc gtcggcctga gcctgcactt ctggcgcaag
     1021 cgctacatcg ggcgcgtgcc ccactgagtt tggcgccgac ccgcgcgacc cgccacaggc
     1081 tcacttcgcg tacgggtctt cgaacccgag cgcgcgcatc agctcggact cgcgcgcttc
     1141 catcgcgcgc gcctcggcgt cgagctcatg gtcgtagccc tgcgcgtgca gcgtgccgtg
     1201 cacgagcatg tgcgcgtagt gcgcggcgag cgggatgccg agctcatgcg cctcgcgctg
     1261 cagcaccggc gcgcacagca ccaggtcggc actgacgacg ggtgcacggc tgtagtcgaa
     1321 ggtcagcacg ttggtcgcgt agtccttcgc gcggtattcg cggttcagcg cccggccttc
     1381 gtcggcaccg acgatgcgca ccgtgatctc gcccggcgct tcgagcgccg cgcgaatcca
     1441 gcgcgcgacc cggtggcgcg gcagcacggc gcggtcgctc gcgtcggcga actgcaacga
     1501 cagggtcagc ggcgggccga gcgccgggcc gctcccaagc cggcccgcat ccccgcgggg
     1561 gatcgtccga cgtacccgtc ggacgagggg cttcatttca ggcttcatcc aggcgcgcgg
     1621 cgtcgtaggc ctcgacgatc cgcgctacca gcgggtggcg caccacgtcg gacgaggtga
     1681 agcgcgtggt cgcgatgccg cgcacgcggc gcagcacgcg ctcggcgtcg atcaggccgc
     1741 tctcggtgcc gcgcggcagg tcgatctggc tcacatcgcc gttgatcacg accttggtac
     1801 cgaagccgat gcgcgtcagg aacatcttca tctgctcgac cgtgctgttc tgcgcctcgt
     1861 cgaggatcac gaacgcgtgg ttcagcgtgc gcccgcgcat gaacgcgagc ggcgcgatct
     1921 cgagcgtgcc cttctcgaac gccttcgcga ccttgtcggc gcccatcagg tcgtacagcg
     1981 cgtcgtagag cgggcgcagg taggggtcga ccttctgcgc caggtcgccg ggcaggaagc
     2041 cgagccgctc gccggcctcg accgccgggc gcgtcaggat cacgcgctgc accgcgctgc
     2101 gctccaacgc gtcgaccgcg caggcgaccg cgagaaaggt cttgccggtg ccggccgggc
     2161 cgatgccgaa ggtgatgtcg tgcgcgagga tgttgcgcag gtaggtgacc tggttcggcg
     2221 tgcggccggc cagctcggcg cggcgcgtgc gcagcgtcgg cgtgtcgcca cgcgcggcct
     2281 cgggcagcgc cgcgttggcg ccgacctcga ccagcgcgag ctgcagctgc tcggcgtcga
     2341 tcggccgctg cgcctgctcg tacagtgcca gcagcaggtt catcgcgtgc gcggcggccg
     2401 gcttcggccc ctcgatgcgg aagaactcgt tgcgccgcga gacgctgacg tcgagcagcc
     2461 gctcgatgct gcgcaggtgc tcgtcgaggc tgccgcacag gtgcgcgagg cgggcgttgt
     2521 cgcgcggttc gaaggcgtgg cgaaggatca aggcggtggg cgtgcggttc ggagcgccgg
     2581 attgtcgccc gacggcgtgc ctttgtcggc accggcgcgc ggtttggtgt tgcgtgggcg
     2641 ctcgtcgcgg cgggggcgtc cggcacaatc cgcgtcgatg ccgaaacccc ctgctcccct
     2701 cgctgcaatc cggtggctcg gccgccgctg ccttgccggg tggctggcgc tggtcggcgt
     2761 cctcgcgatc tgcgcgccgg cgctcggcgc cgacgcgcag atcgtcacga tcgaccgcgc
     2821 cgtctcggtc cagcaaatgg tcgagccggg gtcggtgcag cagctcgcgc tgccggtcaa
     2881 gctgcccgac gactgggcgc tgacgcggcc ccgcttcgag ggcagcgtct ggtaccgaac
     2941 cggtttcgac cggccgaccg gcgtcgactc gcacgagctg atg
//
LOCUS       DASRDG010000685         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_680608, whole genome shotgun sequence.
ACCESSION   DASRDG010000685 DASRDG010000000
VERSION     DASRDG010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680608"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /gene="lptE"
                     /locus_tag="VFA35_11545"
     CDS             <1..469
                     /gene="lptE"
                     /locus_tag="VFA35_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HZT56854.1"
                     /translation="ALALPLVAGCGFELRRAPEMRFRSVQLVGFKPRSPLADELRMNI
                     NASTTTLVVAGLAQAQVVLEALDDAREKVVVASNSVGQVTEFQLRERFAFRLRSVNGR
                     ELIPRTEILQNRDLSYTESAALAKEQEETALFRAMQSDIVSQVMRRLASVQNF"
     gene            469..1566
                     /gene="holA"
                     /locus_tag="VFA35_11550"
     CDS             469..1566
                     /gene="holA"
                     /locus_tag="VFA35_11550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZT56855.1"
                     /translation="MQIRPEHLAAHLTKGLRPIYTVWGDEPLLAQEAGDAIRAAARAS
                     GCTERQVHTVAGAHFDWSGLLGAALAMSLFAQRQLIEIRIPSGKPGKDGSEALQRYCE
                     ALSSDVVTLVQLPRLDRSQQGSAWFGALDAAGVTVRVDPIERKALPQWIAQRLAQQGQ
                     RVAGGEDGQRTLAFFADRIEGNLLAAHQEIQKLGLLYPAGELGFEQIEAAVLNVARYD
                     VFKLGEAVLAGQVARALRMLAGLQAEGEAAVLVHWTLTEDIRALKRVQDALAAGKPLP
                     LALREARVWGAKERLFERVVPMLTEHAVAHLLEAAQVCDGLVKGLKHPDWPQDPWDGL
                     KRLVLMLVQHTSAAPAAGRGRAVVQRLALQA"
     gene            complement(1576..>2214)
                     /locus_tag="VFA35_11555"
     CDS             complement(1576..>2214)
                     /locus_tag="VFA35_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZT56856.1"
                     /translation="RMVDPRMNATMAQTALWAVASLQRGFFDYAAQQRERVWKQHALR
                     HADEVTVAVLGMGQMGRATAQLLAQQGYRVLGWHRGAGPLAPVLAQADVVINLLPLTP
                     ATHGLFNAATFAQMRRGASLVNLARGAHVVDADLLAALAGGQLRRAVLDVFATEPLPA
                     SHPFWTHPAITVLPHVAAPTDPRSAAAIAARNIAALRRGEALQHLVDHTRGY"
BASE COUNT          306 a          793 c          826 g          289 t
ORIGIN      
        1 tgcgctcgcg ctgccgctcg tcgccggctg cgggttcgag ctgcggcgcg cgcccgaaat
       61 gcgctttcga tcggtgcagc tggtcggctt caagccccgt tcgccgctcg cggacgagct
      121 gcgcatgaac atcaacgcga gcacgacgac gctggtcgtc gccggcctgg cgcaggcgca
      181 ggtcgtgctc gaggcgctcg acgacgcgcg cgagaaggtc gtcgtcgcga gcaactcggt
      241 cgggcaggtc acggaattcc agctgcgcga gcgattcgcg ttccggctgc gcagcgtcaa
      301 tgggcgcgag ctgatcccgc gcaccgagat cctgcagaac cgcgacctca gctacaccga
      361 aagcgccgcg ctcgcgaagg agcaggaaga aaccgcgctg ttccgcgcga tgcagagcga
      421 catcgtctcg caggtgatgc ggcgcctcgc gtcggtgcag aacttctgat gcagatccgc
      481 cccgagcacc tcgccgcgca cctgaccaag ggcctgcgcc cgatctacac cgtctggggc
      541 gacgagccgc tgctcgcgca ggaggccggc gacgcgatcc gcgccgcggc gcgcgccagc
      601 ggctgcaccg agcgccaggt gcacaccgtc gccggcgcgc acttcgactg gagcgggctg
      661 ctcggcgccg cgctggcgat gagcctgttc gcgcagcgcc agctgatcga gatccgcatt
      721 ccgtcgggca agccgggcaa ggacggctcg gaagcccttc agcgctactg cgaggccctg
      781 agcagcgacg tggtcacgct ggtgcagttg ccccggctcg accgcagcca gcagggcagc
      841 gcgtggttcg gcgcgctcga cgccgcgggc gtcacggtgc gcgtcgaccc gatcgagcgc
      901 aaggcgctcc cgcagtggat cgcgcagcgg ctcgcgcagc agggccagcg cgtggccggc
      961 ggcgaagacg gccagcgcac gctcgcgttc ttcgccgacc gcatcgaggg caacctgctc
     1021 gccgcgcacc aggagatcca gaagctcggc ctgctctacc cggcgggcga gctcggcttc
     1081 gagcagatcg aggcggcggt gctgaatgtg gcgcgctacg acgtgttcaa gctcggcgag
     1141 gcggtgctcg ccggccaggt cgcgcgcgcg ctgcgcatgc tggccgggct gcaggccgag
     1201 ggcgaggccg ccgtgctcgt gcactggacg ctgaccgagg acatccgcgc gctcaagcgc
     1261 gtgcaggacg cgctcgccgc cggcaagccg ctgccgctcg cgctgcgcga ggcgcgcgtc
     1321 tggggcgcga aggagcggct gttcgagcgc gtcgtgccga tgctgaccga gcacgcggtc
     1381 gcgcacctgc tcgaagcggc gcaggtctgc gacggcctcg tcaagggcct gaagcatccc
     1441 gactggccgc aggacccgtg ggacggcctg aagcggctcg tgctgatgct ggtgcagcac
     1501 acctcggcgg cgcccgccgc cggccgcggc cgcgccgtcg tgcagcggct cgcactgcag
     1561 gcctgaccag gcgcgtcagt agccgcgcgt gtggtcgacg aggtgctgca gcgcttcgcc
     1621 gcggcgcagc gcagcgatgt tgcgcgcagc gatcgcggcc gcactgcgcg ggtcggtcgg
     1681 cgcggcgacg tgcggcagca cggtgatggc cgggtgcgtc cagaacgggt gcgacgcggg
     1741 cagcggctcg gtcgcgaaca cgtcgaggac ggcacgccgc agctggccgc ccgcaagcgc
     1801 ggcgagcagg tccgcgtcga cgacgtgggc gccgcgcgcg aggttcacga ggctcgcgcc
     1861 gcggcgcatc tgcgcgaagg tcgcggcgtt gaacaacccg tgcgtcgcgg gcgtcagcgg
     1921 cagcaggttg atcacgacat cggcctgcgc gagcaccggt gcgagcgggc cggcgccgcg
     1981 gtgccagccg agcacgcggt agccctgctg cgccagcagc tgcgcggtcg ctcggcccat
     2041 ctggcccatg cccagcaccg cgaccgtcac ttcatctgcg tgccgcagcg cgtgctgctt
     2101 ccagacgcgc tcgcgctgct gcgccgcgta gtcgaagaag ccgcgctgca ggctcgccac
     2161 cgcccacagc gcggtctgcg ccatcgtcgc gttcatcctg gggtcgacca tgcg
//
LOCUS       DASRDG010000686         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1713894, whole genome shotgun sequence.
ACCESSION   DASRDG010000686 DASRDG010000000
VERSION     DASRDG010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713894"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /locus_tag="VFA35_11560"
     CDS             <1..1878
                     /locus_tag="VFA35_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56857.1"
                     /translation="DVDRIDDGENRLPRLPLLSRDGARLLLLGYDGALLGCELAADGA
                     HWRLDQPGWWAQQGAFAPDGRTFVVRWQHGRQQQGAEDVRLYDSATGRELRRLELGGQ
                     ETMCCAFAADGSRLAVGGMDGVVRVFDTAGWQPVAELRAGGEDLAHVYWVGFAAGGAQ
                     LVALGWDGLSVWDLGSRARVAHHVAADARPFHVGAFSDDGRRFAAVVKDGSVRFYETA
                     AWTEIGSARWTHRYPNELVWNHRGDAVAFQDGRGVQVAGLQAPAPELRPHRDAIVSLE
                     FLADGRHVLTASRDATAAVVDLDSGLAEPVFPHPAPVRRARLSGDGARVVTAGEDGVV
                     RVFDRRGGAPQLALPLHDGPAIDARFVAGDARLLSIGRDGRVQYVDAATGRAVATLRA
                     HRQACLALCVDEQLGLIATGGADHRVLVCDLAGAPVADLSTLPPGDAPGLDIQGNAEA
                     LAFDAARRRLVVANRRDALLVFAIDGWQPTWVRSRTQGQEYSSHLALAPGSAFYATAH
                     SGVGDWTFVDAATLEPADVGAAGFPSAIVSSLRFSPDGRLLLVASRDDTVSLWDLARR
                     ERQLELRAQHGGVRSTAFDRAGEWVATGSQDGTLRAWPVHPLPFAERHWARLAGRPAG
                     P"
BASE COUNT          217 a          779 c          797 g          253 t
ORIGIN      
        1 gacgtcgacc gcatcgacga cggtgagaac cggctgccgc ggctgccgct gctgtcgcgc
       61 gacggcgcgc ggctgctgct gctcggctac gacggcgcgc tgctgggctg cgagctggcc
      121 gccgacggcg cgcactggcg cctcgaccag cccggctggt gggcgcagca gggcgcgttc
      181 gcgccggacg gccgcacgtt cgtcgtgcgc tggcagcacg gccgccagca gcagggcgcc
      241 gaggacgtgc gcctctacga cagcgccacc gggcgggagc tgcggcggct cgagctcggc
      301 ggccaggaga ccatgtgctg cgcgttcgcg gccgacggca gccggctcgc ggtcggcggc
      361 atggacggcg tggtgcgcgt gttcgacacg gccggctggc agccggtcgc cgaactgcgc
      421 gccggcggcg aggacctggc gcacgtctac tgggtcggct tcgcggccgg cggcgcgcaa
      481 ctggtcgcgc tgggctggga cgggctgtcg gtgtgggacc tcggcagccg cgcgcgcgtc
      541 gcgcaccacg tcgcggccga cgcgcggccg ttccacgtcg gcgcgttcag cgacgacggc
      601 cggcgcttcg cggcggtcgt caaggacggc agcgtgcgct tctacgagac ggcggcgtgg
      661 accgagatcg gcagcgcgcg ctggacccac cgctacccga acgagctggt ctggaaccac
      721 cgcggcgacg cggtcgcgtt ccaggacggg cgcggcgtgc aggtcgcggg cctgcaggcg
      781 ccggcgcccg agttgcggcc gcaccgcgac gcgatcgtgt cgctcgagtt cctggccgac
      841 ggccggcacg tgctgaccgc gtcgcgcgac gcgaccgccg ccgtcgtcga cctcgactcg
      901 gggctcgccg agcccgtgtt cccgcatccg gcgccggtgc ggcgcgcgcg cctctcgggc
      961 gacggcgcgc gcgtcgtcac ggccggcgag gacggcgtcg tgcgcgtgtt cgaccgccgc
     1021 ggcggcgcgc cgcagctggc gctgccgttg cacgacggtc ccgcgatcga cgcgcgcttc
     1081 gtcgccggcg acgcacggct gctcagcatc ggccgcgacg gccgcgtgca gtacgtcgac
     1141 gcggccacgg gccgtgccgt cgcgacgttg cgcgcgcacc ggcaggcgtg cctcgcgctc
     1201 tgcgtcgacg agcagctggg cctgatcgcg accggcggcg ccgaccaccg cgtgctcgtc
     1261 tgcgacctcg ccggcgcgcc ggtcgccgac ctgtcgacgc tgccgcccgg cgacgcgccg
     1321 gggctcgaca tccagggcaa tgccgaagcg ctcgcgttcg acgcggcgcg gcggcggctc
     1381 gtggtcgcca accggcgcga cgcgctgctg gtgttcgcga tcgacggctg gcagccgaca
     1441 tgggtgcggt cgcgcacgca gggccaggag tactcgagcc acctggcgct cgcgcccggc
     1501 agcgccttct acgcgaccgc gcacagcggc gtcggcgact ggaccttcgt cgacgccgcg
     1561 acgctcgagc ccgccgacgt cggcgccgcg ggcttcccga gcgcgatcgt ctcctcgctg
     1621 cggttctcgc ccgacggccg gctgctgctg gtcgcgtcgc gcgacgacac cgtgagcctg
     1681 tgggacctcg cgcgccgcga acggcagctc gagctgcgcg cgcagcacgg cggcgtgcgc
     1741 agcaccgcgt tcgaccgcgc cggcgagtgg gtcgcgaccg gcagccagga cggcacgctg
     1801 cgcgcgtggc ccgtgcatcc gctgccgttc gcggagcgcc actgggcgcg gctcgcaggc
     1861 cggcccgccg gaccgtgatc agggcagcag ctgtgcggcg ctcggccact cgcgcacgcg
     1921 gtcgcgcagc cgctgccagc gcttctgcac ggcttcgacg ccgatcccga gccgcgccgc
     1981 gacctcggcg tgcggcatcg cctcgagccc gacgtggatc agcagcatgc gctcctcgcc
     2041 gtcgag
//
LOCUS       DASRDG010000687         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1492687, whole genome shotgun sequence.
ACCESSION   DASRDG010000687 DASRDG010000000
VERSION     DASRDG010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492687"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFA35_11565"
     CDS             <1..309
                     /locus_tag="VFA35_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56858.1"
                     /translation="KPVLSSTGAIRLEDLRGDTLMVQIASKSMLLNVRTAQRIVDDCV
                     SPKQRELIEAARVAKAAAAAASAAPAAEAGSAPATAPASPPASAAPAMPTTAPTVQPK
                     "
     gene            390..635
                     /locus_tag="VFA35_11570"
     CDS             390..635
                     /locus_tag="VFA35_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZT56859.1"
                     /translation="MEATVAERGQITLPKAVRDALGLTKGTQLKVELDGGRIILRKNV
                     DDAISRARGRFKLPPGVTTDDVMRELRGRAPGDPIEV"
     gene            635..1057
                     /locus_tag="VFA35_11575"
     CDS             635..1057
                     /locus_tag="VFA35_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZT56860.1"
                     /translation="MIAIDSSVLIDLLGEDAMADAAEACLRDALAHGPVVLCDVVVSE
                     VTAGLGHGAEIMDVVEEMGMSYLAVERKAAIRAGEMQRRYRQRLRALGKDVAVQRTVP
                     DFIVGAHALLQCNALITRDAGFFRDYFKGLKVINPSAA"
     gene            1112..>2765
                     /locus_tag="VFA35_11580"
     CDS             1112..>2765
                     /locus_tag="VFA35_11580"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HZT56861.1"
                     /translation="MLQAYRQHVAERAALGIPPLPLSAQQTSELIELLKAPPPGEEAT
                     LVELITQRVPAGVDDAAKVKASYLAAVAHGTEVTPLISRARATELLGTMLGGYNISPL
                     VDLLDDPVVGTVAAEGLKKTLLMFDQFHDVQEKAEKGNANAKAVLQSWADAEWFTSRP
                     EVPKSITVSIFKVAGEINTDDLSPAPDAWSRPDIPMHALAMHKNARPGIVPEEDGKRG
                     PVKFIEELRARGNLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGG
                     KIAPIFYNTMEDSGALPIELDVSQMNMGDVVELRPYEGKALKDGKVIAEFTVKSDVLF
                     DEVRAGGRIPLIIGRGLTAKAREALGLPPSTLFRLPTNPADTKKGFSLAQKMVGKACG
                     LPVVDGEQQGVRPGTYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHT
                     AAYPKPVDVKMHHELPDFISTRGGISLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFP
                     IGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGTMQPGVTL"
BASE COUNT          475 a          980 c          927 g          383 t
ORIGIN      
        1 aagcccgtgc tgtcgtcgac cggcgcgatc cggctcgaag acctgcgcgg cgacacgctg
       61 atggtgcaga tcgccagcaa gtccatgctg ttgaacgtca ggacggcgca gcgcatcgtc
      121 gatgactgcg tcagcccgaa acagcgcgaa ctgatcgagg cggcgcgggt ggcgaaggcg
      181 gcggccgccg cggcgtcggc ggcaccggcg gccgaagccg gctccgcgcc cgccacggcg
      241 ccggccagcc cgccggcctc ggcggccccg gcgatgccca cgacggcgcc gaccgtgcaa
      301 cccaagtaga cccagcgcgc ccggccgtcc ttgtagtacg catcatttgg tatgtatact
      361 tagcgcatac ttgattccgg gagctgtgca tggaagcaac tgtcgccgaa cgcggtcaga
      421 tcacgctgcc gaaggccgtg cgcgacgcgc tcgggctgac caagggcacg cagctcaagg
      481 tcgagctcga cggcggccgc atcatcctgc gcaagaacgt cgacgacgcg atctcgcggg
      541 cgcgcggcag gttcaagctg ccgcccggcg tgaccaccga cgacgtgatg cgcgagctgc
      601 gcggccgcgc cccgggcgat ccgatcgagg tctgatgatc gcaatcgatt cttcggtgct
      661 gatcgacctc ctcggcgagg acgcgatggc cgacgccgcg gaggcctgcc tgcgcgatgc
      721 cctggcccac ggcccggtcg tgctgtgcga cgtggtcgtc agcgaggtca ccgccggcct
      781 cggtcacggc gccgagatca tggacgtggt cgaggagatg ggcatgagct acctcgcggt
      841 cgagcgcaag gccgcgatcc gcgccggcga gatgcaacgt cgctacaggc agcggctgcg
      901 cgcgctgggc aaggacgtcg cagttcagcg caccgttccc gacttcatcg tcggggcaca
      961 tgccttgctt cagtgcaacg ccctgatcac gcgcgacgcc ggcttcttcc gcgactattt
     1021 caagggcctg aaagtcatca acccgagcgc ggcctgacga ccctgcccac ccgcccccac
     1081 cgcctctaca cgaacaccca aggagctccc gatgctgcaa gcctaccgcc aacacgttgc
     1141 cgaacgcgcc gccctcggca tcccgccgct gccgctgtcg gcccagcaga ccagtgagct
     1201 gatcgagctg ctgaaggcgc cgccgcccgg cgaagaagcc acgctggtcg agctgatcac
     1261 gcagcgcgtg ccggccggcg tcgacgacgc ggccaaggtc aaggcgagct acctcgcggc
     1321 ggtggcacac ggcaccgagg tcacgccgct gatctcgcgc gcgcgcgcca ccgagctgct
     1381 cggcacgatg ctcggcggct acaacatcag cccgctggtc gacctgctcg acgatcccgt
     1441 cgtcggcacg gtcgccgccg aaggcctgaa gaagacgctg ctgatgttcg accagttcca
     1501 cgacgttcag gagaaggccg agaagggcaa cgccaacgcc aaggcggtgc tgcagagctg
     1561 ggccgatgcc gagtggttca cctcgcgccc cgaggtgccg aagtcgatca ccgtgagcat
     1621 cttcaaggtc gccggcgaga tcaacaccga cgatctctcg cccgcgcccg acgcctggag
     1681 ccgccccgac atcccgatgc acgcgctggc gatgcacaag aacgcgcgcc ccggcatcgt
     1741 gccggaagaa gacggcaagc gcggcccggt caagttcatc gaggaactgc gcgcgcgcgg
     1801 caacctcgtc gcctacgtgg gcgacgtcgt cggcaccggc tcgtcgcgca agtcggcgac
     1861 caactcggtg ctgtggttca ccggcgaaga catcccgttc gtgccgaaca agcgcttcgg
     1921 cggcgtctgc ctcggcggca agatcgcgcc gatcttctac aacacgatgg aagactcggg
     1981 cgcgctgccg atcgagctcg acgtgagcca gatgaacatg ggcgacgtgg tcgagctgcg
     2041 cccctacgaa ggcaaggcgc tgaaggacgg caaggtgatc gccgagttca cggtcaagag
     2101 cgacgtgctg ttcgacgaag tgcgcgccgg cggccgcatc ccgctgatca tcgggcgcgg
     2161 cctgaccgcg aaggcgcgcg aagcgctggg cctgccgccg agcacgctgt tccgcctgcc
     2221 gaccaacccg gccgacacca agaagggctt cagcctggcg cagaagatgg tcggcaaagc
     2281 ctgcgggctg ccggtcgtcg acggcgagca gcaaggcgtg cgccccggca cctattgcga
     2341 accgcgcatg accagcgtcg gctcgcagga caccaccggc ccgatgacgc gcgacgagct
     2401 gaaagacctc gcctgcctcg gcttcagcgc cgacctggtg atgcagagct tctgccacac
     2461 cgcggcctac ccgaagccgg tcgacgtgaa gatgcaccac gaactgcccg acttcatcag
     2521 cacccgcggc ggcatctcgc tgcggcccgg cgacggcgtg atccactcgt ggctgaaccg
     2581 cctgctgctg cccgatacgg tgggcaccgg cggagattcg cacacgcgct tcccgatcgg
     2641 catcagcttt ccggcgggct cgggccttgt ggctttcgcg gccgccaccg gcgtgatgcc
     2701 gctggacatg cccgagagcg tgctggtgcg cttcaagggc acgatgcagc cgggcgtgac
     2761 gctgc
//
LOCUS       DASRDG010000688         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_110091, whole genome shotgun sequence.
ACCESSION   DASRDG010000688 DASRDG010000000
VERSION     DASRDG010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110091"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      162..268
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            326..2476
                     /locus_tag="VFA35_11585"
     CDS             326..2476
                     /locus_tag="VFA35_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HZT56862.1"
                     /translation="MARLTPRFHTLCPIAAAAWFVATHAVAQTAPAPTQLEPITVTGR
                     TTPPATVSGWGDTPLAKTPLQASVFGSEQLKDGGVQRLSDLTAVDAGVSDAYNAEGYW
                     DFLTVRGYVLDNRFNFRRDGLPINAETSIPLDNKARIEILKGTSGMQAGTSAPGGLVN
                     YVVKRPLDAPLRSVSLEWRQPGSVTGALDLSQRFGAGDAFGVRLNAAAAHLDPMVRDS
                     KGNRNLLALAADWRISPDSLLEAEVETSHRAQPSQPGFSMLGNTVPQPGDPRLSLNNQ
                     PWSLPVVFDATTASLRWQQKLGKDWRFVAHAATQQLRTDDRLAFPFGCSKESNYDRYC
                     SDGSFDYYDFQSDNERRRTDTLQLSMEGQATTGTLKHRLGFGVLESRFRSRLQPRVND
                     GIIVGEGTVDGLTQISTLPVLDMVPNTDLTERSTELFFRDAIALSERLTAWVGVRHTR
                     IHRESVRTDGTVATAPFAQSFTTPSIALGYAFARDQLVYASWGQGVESNAVPNRSIYS
                     NAGAVFTTKSRQAEVGLKGAGGAFEWNLAAFDIRRPRTQDFGSCDADATCTTRLDGTA
                     HHRGIEANAAWRQGPWTLRGGAQWLRARVEGSADPTVDGKQPTNVPERTLKLQAGYDV
                     AAVRGLNLQAGLTHEGARMVLPDNSATIPAWTRVDAALRYETKLAGAQTTWRAGIDNL
                     FDKRAWRESPYEFSHVYLYPLAPRTLRVSLQIDL"
     gene            2512..2587
                     /locus_tag="VFA35_11590"
     tRNA            2512..2587
                     /locus_tag="VFA35_11590"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2545..2547,aa:Asn,seq:gtt)
BASE COUNT          445 a          969 c          826 g          347 t
ORIGIN      
        1 tcgccgcctt cggcgccgcg gcgatcgtgt ttcccgacca gccggtgcac ctcgaataca
       61 cgaccaccag cccgcgcctg caccgggcgc tcggcgtcgc gcccgcgacc gcagcagggg
      121 cggctgggtc ataattcaga ccccgcgcgg tcatgccgcg cggcattcgc taggggtgtc
      181 gaacggttct cccgaaccgc tcggctgaga aagtcccttt gaacctgatt gaggtaatcc
      241 tcgcgcaggg aagcatggcc gaagcggcgc cgcgttctgc ggctcgccgc cggcccgccg
      301 acctgccatc gaccacggaa ccacgatggc acgcttgacc ccacgctttc acacactctg
      361 cccgatcgcg gccgctgcat ggttcgtcgc gacgcatgcg gtggcgcaga ccgcgccggc
      421 cccgacgcag ctcgaaccga tcaccgtcac cgggcgcacg acgccgccgg caacggtcag
      481 cggctggggc gacacgccgc tcgcgaagac gccgctccag gccagcgtct tcggcagcga
      541 acagttgaag gacggcggcg tgcagcgcct gtccgacctg accgcggtcg acgcgggcgt
      601 cagcgacgcc tacaacgccg agggctactg ggacttcctc accgtgcgcg gctacgtgct
      661 cgacaaccgc ttcaacttcc gccgcgacgg gctgccgatc aacgccgaga cctcgatccc
      721 gctcgacaac aaggcacgca tcgagatcct caagggcacg agcggcatgc aggccggcac
      781 gagcgcgccc ggcgggctcg tgaactacgt cgtcaagcgc ccgctcgatg caccgctgcg
      841 ctcggtgagc ctcgaatggc gccagcccgg cagcgtgacc ggcgcgctcg atctgagcca
      901 gcgcttcggt gccggcgacg cgttcggcgt gcgcctgaac gccgcggccg cgcacctcga
      961 cccgatggtg cgcgacagca aaggcaaccg caacctgctc gcacttgccg ccgactggcg
     1021 catcagcccc gactcgttgc tcgaagccga ggtcgagacc agccaccgcg cgcagcccag
     1081 ccagccgggc ttcagcatgc tcggcaacac ggtgccgcag cccggtgatc cgcgcctgag
     1141 cctgaacaac cagccgtggt cgctgccggt cgtgttcgac gcgacgaccg catcgctgcg
     1201 ctggcagcaa aagctcggca aggattggcg cttcgtcgcg catgcggcga cgcagcagct
     1261 gcgcaccgac gaccggctcg cgttcccgtt cggctgctcg aaggagagca actacgaccg
     1321 ctattgctca gacggcagct tcgactacta cgacttccag agcgacaacg agcgccgccg
     1381 caccgatacg ctgcagctct cgatggaagg gcaagccacg accggcacgc tcaagcaccg
     1441 gctcggcttc ggcgtgcttg aatcgcgctt caggagtcgc ctccaaccgc gcgtcaacga
     1501 cggcatcatc gtcggtgaag gcacggtcga cggactcacg cagatctcca cgctgccggt
     1561 gctcgacatg gtgccgaaca ccgacctcac cgagcgctcc accgagctct tctttcgcga
     1621 cgccatcgcc ctgagcgagc gcctgaccgc ctgggtcgga gtgcgccaca cccgcatcca
     1681 tcgcgaaagc gtgcggaccg acgggacggt cgccacggcg ccgttcgcgc aatcgttcac
     1741 gacgccgtcg atcgcgctcg gctacgcgtt tgcgcgcgac caactggtct atgccagctg
     1801 gggccagggc gtcgagtcca acgccgtgcc gaaccgcagc atctactcca acgccggcgc
     1861 ggtcttcacg accaagagcc ggcaggccga ggtcggcctg aagggcgcag gcggcgcgtt
     1921 cgagtggaac ctcgccgcgt tcgacatccg gcgcccgcgc acccaggact tcggcagctg
     1981 cgacgccgac gccacctgca ccacgcggct cgacggcacc gcacaccacc gcggcatcga
     2041 ggcgaacgcg gcgtggcgcc aaggcccatg gacgctgcgc ggcggcgcgc agtggctgcg
     2101 cgcgcgcgtc gaaggcagcg ccgacccgac agtcgacggc aagcagccga ccaacgtgcc
     2161 cgagcgcacg ctgaagctgc aggccggcta cgacgtagcc gcggtccgcg gcctgaacct
     2221 gcaggccggc ctcacgcacg agggcgcacg catggtgctg cccgacaaca gcgcgaccat
     2281 ccccgcgtgg acgcgcgtcg acgccgcctt gcgctacgag accaagctcg caggcgcgca
     2341 gaccacctgg cgcgccggca tcgacaacct gttcgacaag cgcgcctggc gcgagtcgcc
     2401 ctacgagttc agccacgtct acctctaccc gctcgcgccg cgcaccctgc gcgtctcgct
     2461 gcagatcgat ctctgaggca ccaagaaaag ccagctataa tgcaaggctg ttcctcgata
     2521 gctcagtcgg tagagcgccg gactgttaat ccgtaggtcc ctggttcgag cccaggtcgg
     2581 ggagcca
//
LOCUS       DASRDG010000689         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1035424, whole genome shotgun sequence.
ACCESSION   DASRDG010000689 DASRDG010000000
VERSION     DASRDG010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035424"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFA35_11595"
     CDS             <1..199
                     /locus_tag="VFA35_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56863.1"
                     /translation="VIAALADLRLPLDPALAAAAAQRYVLRIELERDVLPGALRLPAL
                     IDADWRLSTGDYAWQARSGAR"
     gene            172..2370
                     /locus_tag="VFA35_11600"
     CDS             172..2370
                     /locus_tag="VFA35_11600"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZT56864.1"
                     /translation="MAGPIRRALSFVARRVLPALAIAALLAATLLLARDAAGDTNRLG
                     RFYPWILGASALALLVLIAIIAQRLLRLSRELKRSVPGARLTRRWLLMLIALAVPPVL
                     VVYGFSLYFLNATIDTWFNARLEQGLSAALGVGKAYLDEHLRAAETSAANIARDLANL
                     PDADLQQRLNAALDAQPITQLVVFGDNRQVLANAGADPRFLDAVYPDANALNQLKITG
                     RYAVAEPLGEPIVLKLRVVLPLADATLGSSRLLQALYPVPEQMQPLMATVEQAGYDFQ
                     RLKFLRDSLKLTFTLVLTFVLLLSALFAVLTAFGVARRLTAPIGRLAAATRAVGAGRY
                     DTPLPVAGDDELGFLTQSFNQMQGELERASSRLAHSAQETDSQRTYLRAVLERLSAGV
                     LGLDREGVLRTANHAAETILDLPLSRHIGQHLSVLRRERPALAPLIDPILKHMREAAR
                     EWREEVVLDDPDQRSGNERRVLMLRGAELSPMLGEDAGIVAVFDDLTLLNRAQRDAAW
                     AEVARRLAHEVKNPLTPIQLAAERLRRRFIGRLPPEETEILDRATLTIVNQVEALKAM
                     VNAFGDYARPPQIDARALSINALASEVVDLYENDQRIQLTRQFAENDPSLRADAVRLR
                     QLLHNLIKNSLEAVTGEHKPHIEVITREIDEGGRHWVELAVADNGPGLPEGFDARWFE
                     PYTTSKVKGTGLGLAVAKKIVEEHGGTIRAENRAEGGAVFTLRLPRDGDV"
     gene            2510..>4007
                     /locus_tag="VFA35_11605"
     CDS             2510..>4007
                     /locus_tag="VFA35_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZT56865.1"
                     /translation="MPPSPARRELWLFLLFALVVIGAGIGLRDPWPADEPRFALSAQQ
                     MVASGDWLIPHRGHEIYADKPPAFMAMQAAAFELVRNWRIAFLLPSLLAALGTLWLVY
                     DLGRRLWNHRTGLYAGVALLCVFQFVYQAKRAQIDPMVTFFITLANYGLLRHFLLGPD
                     WRMYWLGCFAAGLGVITKGVGVLALLMFVPYIFARSIHWNFVATTKDAAWRWAGGLAA
                     LLAAIALWLAPLALAVSLHPASEYQAYLHDILFRQTAARYTDSWDHQQPPWYYLGVIL
                     SGWMPLWLTWFGTVPAWVRALRERDARILLPLAWSLLVIAFFSIPAGKRDMYIMPALP
                     MVALACSPYLPQIVERRWFRVLALALLVVLGAMLAGAGLHAWSGHWKFANDLAAQRGF
                     DGGGAALWGFLIALGAWLLACAAWFRFARAAHGLVAGFAGLWLLWGLWASPLLNDSSS
                     ARGLMQQAGAMIGPDAELALVAWKEQNLLFADRKVVEFGFVKPPHLQLA"
BASE COUNT          550 a         1432 c         1313 g          712 t
ORIGIN      
        1 cgtcatcgcc gcgctcgccg acctgcgcct gccgctcgat ccggcgctgg ccgccgcggc
       61 ggcgcagcgc tatgtgctgc gtatcgagct cgaacgcgat gtcttgcccg gcgcgctgcg
      121 cctgccggcg ctgatcgacg cggattggcg cctttccacc ggggactacg catggcaggc
      181 ccgatccggc gcgcgctgag cttcgtcgcg cggcgggtgc tgcccgcgct cgccatcgcc
      241 gccctgctgg cggcgacgct gctgctcgcg cgcgacgcgg caggcgacac gaaccggctc
      301 gggcgcttct atccgtggat cctcggtgcc tcggcgctgg cgctgctcgt gttgatcgcg
      361 atcatcgcgc aacgcctgct gcggctgtcg cgcgaactca agcgctcggt gccgggcgcg
      421 cgcctgaccc ggcgctggct gctcatgctg atcgcgctcg ccgtgccgcc cgtactcgtg
      481 gtctacggct tctcgctcta cttcctcaat gcgacgatcg acacctggtt caacgcgcgc
      541 ctcgagcaag gcctgtccgc cgcgctcggc gtcggcaagg cctacctcga cgagcatctg
      601 cgtgcggccg agacgagcgc ggcgaacatc gcgcgcgatc tggccaacct gcccgacgcc
      661 gacctgcaac agcgcctcaa cgccgcgctc gatgcgcagc cgatcaccca gctcgtcgtg
      721 ttcggcgaca accgccaggt gctcgccaac gccggtgccg atccgcgctt cctcgacgcg
      781 gtttacccgg acgcgaacgc actgaaccag ctcaagatca ccggccgtta cgccgtcgcc
      841 gagccgctcg gcgagccgat cgtgctcaag ttgcgcgtgg tgttgccgct cgccgatgcc
      901 accctcggtt cgtcgcggct gctgcaggcg ctgtatcccg tgcccgagca gatgcagccg
      961 ctgatggcga cggtggaaca ggccggctac gacttccagc gcctgaaatt cctgcgcgat
     1021 tcgctcaagc tgaccttcac cctcgtgctg accttcgtgc tgctgctgtc ggcgctgttc
     1081 gcggtgctga ccgcgttcgg cgtcgcgcgc cggctgaccg caccgatcgg ccgcctcgcc
     1141 gcggcgacgc gcgcggtcgg cgcgggccgc tacgacacgc cgctgccggt cgcgggcgac
     1201 gacgagctcg gcttcctcac ccagtcgttc aaccagatgc agggcgagct cgagcgcgcg
     1261 agctcgcgtc tcgcccacag cgcgcaggag accgacagcc agcgcacgta cctgcgcgcc
     1321 gtgctcgagc gcctgtccgc cggcgtgctc ggcctcgacc gcgaaggcgt gttgcgcacg
     1381 gccaaccatg ccgccgagac gatcctcgac ctgccgctgt cgcgccacat cggtcagcat
     1441 ctgtcggtgc tgcgccgcga acgccccgcg ctcgcaccgc tgatcgatcc gatcctcaag
     1501 cacatgcgcg aggccgcgcg cgaatggcgc gaagaggtcg tgctcgatga tccggatcag
     1561 cgctcgggca acgagcgccg cgtgctcatg ctgcgcggcg ccgaactctc gccgatgctc
     1621 ggcgaggatg ccggcatcgt cgccgtgttc gacgacctga ccctgctcaa ccgcgcccag
     1681 cgcgatgcgg cctgggccga ggttgcgcgt cgcctcgccc acgaagtgaa gaatccgctg
     1741 acgccgatcc agctcgcggc cgaacgcctg cgccgccgtt tcatcggccg cctgccgccc
     1801 gaggaaaccg aaatcctcga ccgcgccacg ctgaccatcg tcaaccaggt cgaggcgctc
     1861 aaggccatgg tcaacgcgtt cggcgactac gcgcggccgc cgcaaatcga cgcacgcgcg
     1921 ctgtcgatca atgcgctcgc cagcgaggtg gtcgatctgt acgagaacga ccagcgcatc
     1981 cagcttacgc gtcaattcgc agagaacgat ccgagcctgc gtgccgacgc ggtgcgcctg
     2041 cgccagctgc tgcacaacct gatcaagaac tcgctcgaag ccgtcaccgg cgagcacaag
     2101 ccgcacatcg aggtgatcac gcgcgagatc gacgaaggcg gccgccattg ggtcgaactc
     2161 gccgtcgccg acaacggccc gggtcttccc gagggcttcg acgcgcgctg gttcgagccg
     2221 tacacgacga gcaaggtcaa gggcacgggc ctgggcctgg cggtcgcgaa gaagatcgtc
     2281 gaggaacacg gcggcacgat ccgcgccgag aaccgtgccg aaggcggtgc cgtgttcacc
     2341 ctgcgcctgc cgcgcgacgg cgatgtctga gaacaggttt tgacgaagac caaaccggcc
     2401 ggcgtcagcg cggcgtcaac ttgtgttaag taggctcgac cgtgcgcacg agcgcatgcc
     2461 gcttttccgt cactccgttt cttcgatcac cgcaatgagc gttccatcga tgccaccttc
     2521 acctgcccgc cgcgaactgt ggttgttcct gttgttcgcc ctcgtcgtca tcggcgccgg
     2581 catcggcctg cgcgatccgt ggccggcgga tgagccgcgc ttcgcgttgt cggcgcagca
     2641 gatggtcgcc tcgggcgact ggctgatccc gcatcgcggc cacgagatct acgccgacaa
     2701 gccgcccgcg ttcatggcca tgcaggcggc cgcgttcgag ctcgtgcgca actggcgcat
     2761 cgccttcctg ctgccctcgt tgctcgccgc gctcggcacg ctgtggctgg tctatgacct
     2821 cggccggcgg ctctggaacc accgcactgg cctctacgcc ggcgtcgccc tgctctgcgt
     2881 gttccagttc gtctaccagg ccaagcgcgc gcagatcgat ccgatggtga cgttcttcat
     2941 caccctcgcc aactacggtc tgctgcggca tttcctgctt ggccctgact ggcgcatgta
     3001 ctggctcggc tgtttcgccg cgggccttgg cgtgatcacc aagggtgtcg gcgtgctcgc
     3061 gctgctgatg ttcgtgccgt atatttttgc gcgaagtata cattggaact ttgtcgcaac
     3121 gaccaaggat gccgcctggc gctgggccgg cggactcgcc gcgctgctcg ccgcgatcgc
     3181 gctgtggctt gcaccgcttg cgctggcggt gtcgctgcat cccgcatcgg agtaccaggc
     3241 ctatctgcac gacatcctgt tcaggcagac ggcggcgcgc tacaccgatt cgtgggatca
     3301 ccagcagccg ccgtggtatt acctcggtgt gatcctgagc ggctggatgc cgctgtggct
     3361 gacctggttc ggcaccgtgc cggcgtgggt gcgcgcgctg cgcgagcgcg atgcgcgcat
     3421 cctgctgccg ctagcctggt cgctgctcgt gatcgcgttc ttcagcattc ccgcgggcaa
     3481 gcgcgacatg tacatcatgc cggcactgcc gatggtcgcg ctggcctgct cgccgtatct
     3541 gccgcagatc gtggaacggc gctggttccg cgtgctcgcg ctggcgctgc tcgtcgtgct
     3601 cggcgcgatg ctcgccggcg ccggcctgca cgcctggtcg ggacactgga aatttgccaa
     3661 cgacctcgcc gcacagcgtg gtttcgacgg cggtggcgcg gcgctgtggg gttttctgat
     3721 cgcgctgggc gcgtggttgc tggcctgcgc cgcctggttc cgtttcgcgc gcgccgcgca
     3781 tgggctagtc gcgggtttcg ccgggctgtg gctgctctgg ggcctgtggg cgagtccgtt
     3841 gctcaatgat tccagttccg cgcgcggcct gatgcagcag gccggtgcga tgatcgggcc
     3901 cgatgccgaa ctcgcgctgg tcgcgtggaa agaacagaac ctgctgtttg ccgatcgcaa
     3961 agtcgtcgaa ttcggtttcg tcaaaccccc gcacctgcaa ctggccg
//
LOCUS       DASRDG010000690         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1371748, whole genome shotgun sequence.
ACCESSION   DASRDG010000690 DASRDG010000000
VERSION     DASRDG010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371748"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFA35_11610"
     CDS             <1..229
                     /locus_tag="VFA35_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HZT56866.1"
                     /translation="QPIVQALLDHGDHYLLLADYESYVATQLKVDALYRDPGQWAERA
                     IANVAGMGMFSSDRTIREYAREIWGIAPELR"
     gene            271..>2185
                     /gene="glgB"
                     /locus_tag="VFA35_11615"
     CDS             271..>2185
                     /gene="glgB"
                     /locus_tag="VFA35_11615"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZT56867.1"
                     /translation="MLRDNEIDSLSHAAHGDPFAVLGIHADASGQRWLRVMLPGAAQV
                     AVLNASNGELLGTLTSRRADGFFEGPLNTDEHPNYRLQVRWNDGHSAIVDDPYRFPPV
                     LGELDVWLLGEGSHLRPFEVLGAAQRVMLGVAGTSFAVWAPNASQVSVVGDFNFWDAR
                     RHPMRLRRECGVWEIFLPGVGAGAKYKYQIRARDGHVLPQKADPYALQSELRPATASV
                     VARMPSAVPKSEARERANALDAPVSIYEVHLASWRRKHDQGSGPDGRWLNWDELASEL
                     VPYARDMGFTHLELLPISEYPFDGSWGYQPIGLYAPTARFGDAAGFRRFVDRCHAEGL
                     GLLLDWVPAHFPSDAHGLADFDGTHLYEYADPREGFHQDWNTLIYNFGRTEVSNFLVG
                     NALYWLERYNVDGLRVDAVASMLYRDYSRKAGEWIPNEHGGRENLEAIAFLRRMNEVV
                     GGERPQAVTLAEESTAFPGVSRPTYAGGLGFHYKWNMGWMHDTLQYIERDPIYRKHHQ
                     GEMTFSLVYAFTEQFVLPLSHDEVVHGKGSLLSRMPGDDWQKHANLRALYGYMWAHPG
                     KKLLFMGGEFAQRSEWNHDQSLDWHLLQSGMHSGVAALVRDLNRQLREHPALHELDGD
                     GRGFEWLQADSAAA"
BASE COUNT          352 a          762 c          746 g          325 t
ORIGIN      
        1 ccagccgatc gtccaggcgc tgctcgatca cggcgaccac tacctgctgc tcgccgacta
       61 cgagtcgtac gtggcgacgc agctgaaggt cgatgcgctg taccgcgacc cgggccagtg
      121 ggccgagcgc gcgatcgcga atgtggccgg catgggcatg ttctcgtccg accgcacgat
      181 ccgcgagtac gcgcgcgaga tttggggcat cgcgccggaa ttgcgctgaa cccttgttac
      241 gatgcgatgg gcaccacgag aacagacccc atgcttcgag acaacgagat cgactccctc
      301 agccatgccg cgcacggcga cccgttcgcg gtgctgggca tccatgccga cgccagcggc
      361 cagcgctggc tgcgcgtgat gctgcccggc gcggcccagg tggcggtgct gaacgccagc
      421 aacggcgagc tgctcggcac gctgacctcg cgccgcgccg acggcttctt cgagggcccg
      481 ctcaataccg acgagcaccc gaactaccgg ctccaggtgc gctggaacga cggccactcg
      541 gcgatcgtcg acgatcccta tcgcttcccg cccgtgctcg gcgaactcga tgtctggctg
      601 ctcggcgagg gctcgcacct gcgcccgttc gaggtgctcg gcgcggcgca gcgcgtgatg
      661 ctgggcgtgg ccgggaccag ctttgcggtc tgggcgccga acgcgtcgca ggtcagcgtg
      721 gtcggcgact tcaacttctg ggatgcgcgc cgccacccga tgcggctgcg gcgcgaatgc
      781 ggcgtctggg agatcttcct gcctggcgtc ggcgccggcg cgaagtacaa gtaccagatc
      841 cgcgcccgcg acggccacgt gctgccacag aaggccgatc cttacgcgct gcagtccgag
      901 ctgcgcccgg cgaccgcgag cgtggtcgcg cgcatgcctt cggcggtgcc gaagtccgaa
      961 gcgcgcgagc gcgccaatgc gctcgacgcg ccggtcagca tctacgaagt gcacctcgcc
     1021 tcgtggcggc gcaagcacga tcaaggcagc ggccccgacg ggcgctggct gaactgggac
     1081 gaactggcca gcgagctcgt gccctacgcg cgcgacatgg gcttcaccca cctcgagctg
     1141 ctgccgatca gcgagtaccc gttcgacggc tcgtggggct accagccgat cgggctgtac
     1201 gcgccgaccg cacgcttcgg cgacgcagcc gggttccgcc gcttcgtcga ccgctgccac
     1261 gccgaaggcc tggggctgct gctcgactgg gtgccggcgc acttcccgtc cgacgcgcac
     1321 ggcctggccg atttcgacgg cacgcacctg tacgagtacg ccgacccgcg cgaaggattc
     1381 catcaagact ggaacacgct gatctacaac ttcggccgca ccgaggtcag caacttcctg
     1441 gtcggcaacg cgctgtactg gctcgagcgc tacaacgtcg acggcctgcg cgtcgacgcg
     1501 gtcgcctcga tgctctaccg cgactacagc cgcaaggccg gcgagtggat tccgaacgag
     1561 cacggcgggc gcgaaaacct ggaggcgatc gcgttcctgc ggcgcatgaa cgaggtcgtc
     1621 ggcggcgagc ggccgcaggc ggtcacgctg gccgaagaat cgaccgcgtt ccccggcgtg
     1681 tcgcgcccga cctacgccgg cgggctgggc ttccactaca agtggaacat gggctggatg
     1741 cacgacacgc tgcagtacat cgagcgcgat ccgatctacc gcaagcacca ccagggcgag
     1801 atgaccttca gcctcgtcta tgcgttcacc gagcagttcg tgctgcccct gtcgcacgac
     1861 gaggtcgtgc acggaaaggg gtcgctcctg tcgcggatgc cgggagacga ctggcagaag
     1921 cacgccaacc tgcgcgcgct ctacggctac atgtgggcgc atccgggcaa gaagctgctc
     1981 ttcatgggcg gcgagttcgc gcaacgctcg gagtggaacc acgaccagag cctggactgg
     2041 cacctgctcc agtcggggat gcacagcggc gtcgccgccc tggtgcgcga cctcaaccgg
     2101 cagctgcgcg agcaccctgc gctgcacgag ctggacggcg acgggcgcgg gttcgagtgg
     2161 ctgcaggcgg actcggctgc ggcga
//
LOCUS       DASRDG010000691         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_224743, whole genome shotgun sequence.
ACCESSION   DASRDG010000691 DASRDG010000000
VERSION     DASRDG010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224743"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1197
                     /locus_tag="VFA35_11620"
     CDS             64..1197
                     /locus_tag="VFA35_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT56868.1"
                     /translation="MGAAVLLCILPFVVQMAGNAWVRIIDIALLYVLLALGLNIVVGF
                     AGLLDLGYVAFYAVGAYMFGLLASSQLTDTFPAIAALFPNGLHTPWWVIIPLGALLAG
                     AAGVLLGAPTLKLRGDYLAIVTLGFGEIVRVFMNNLDAPVNITNGPKGISGIDDISMF
                     GVHFAKTQTLFGMEFPKVTNYYYLFLALVIIGIVVCYRLERSRIGRAWMAIREDEIAA
                     KAMGINTRNLKLLAFGMGATFGGIAGAMFASFQGFISPESFSLQESVMIVAMVVLGGI
                     GHLPGVIVGALLLSALPEVLRYVAGPLQQMTGGRLDASILRQLLIALAMIVIMLSRPR
                     GLWPSAEHGKDVTPDTLPVDPHVRLTPAEQIPPPVSPIDPSVK"
     gene            1211..1981
                     /locus_tag="VFA35_11625"
     CDS             1211..1981
                     /locus_tag="VFA35_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT56869.1"
                     /translation="MSEIILKVAGVSKRFGGLQALSDVGITITKGQVYGLIGPNGAGK
                     TTFFNVLTGLYTPDSGSFELGGKPYKPTAVHEVAKAGIARTFQNIRLFAEMTALENVM
                     VGRHVRTASGLVGAVLRTRGFKDEEAAIAKRAQELLDYVGIGRFAPYKARTLSYGDQR
                     RLEIARALATDPKLIALDEPAAGMNASEKLVLRELIDRIRRDGRTILLIEHDVKLVMG
                     LCDRVAVLDYGRKIAEGAPASVQRDAAVIAAYLGGALV"
     gene            2023..>2641
                     /locus_tag="VFA35_11630"
     CDS             2023..>2641
                     /locus_tag="VFA35_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT56870.1"
                     /translation="MPDAGKVQPLLEVIGLKVAYGGINAVKGIDFAVAAGEMVTLIGA
                     NGAGKTTTLKALTGLVHPSAGRVRYNGADITALPSHRLVALGIALVPEGRGVFPRLTV
                     EENIDMGAYCRDDKREIRADHEHVLTLFPRLAERHQQAAGTLSGGEQQMLAIARAIMG
                     RPRLLLLDEPSMGLAPLMVQKIFETIRAIAATGVTTLLVEQNAKLA"
BASE COUNT          419 a          890 c          879 g          453 t
ORIGIN      
        1 cgtgaggggc cccaagtgac gcgtttctcc gacgccacgc gccagcagaa gctggtcgtc
       61 ctcatcggcg ccgcggtgct gctctgcatc ctgccgttcg tcgtccagat ggccggcaac
      121 gcctgggtgc gaatcatcga catcgcgctc ctctacgtgc tgctcgcgct cggcctgaac
      181 atcgtggtcg gcttcgccgg gctgctcgat ctgggctatg tcgcgttcta cgcggtcggc
      241 gcctacatgt tcggcttgct cgcctcgtcg caactgaccg acaccttccc ggcaatcgcc
      301 gcgctgttcc cgaacgggct gcacacgccg tggtgggtca tcatcccgct cggcgcgttg
      361 ctcgccgggg cggccggcgt gctgctcggc gcgccgacgc tgaagctgcg cggcgactac
      421 ctcgcgatcg tcacgctcgg cttcggcgag atcgtgcgcg tgttcatgaa caacctcgac
      481 gcgccggtca acatcacgaa cggcccgaag ggcatcagcg gcatcgacga catctcgatg
      541 ttcggcgttc acttcgccaa gacccagacg ctgttcggga tggagttccc gaaggtcacg
      601 aactactact acctgttcct ggcgctggtg atcatcggca tcgtcgtgtg ctaccggctc
      661 gagcgcagcc gcatcggccg tgcctggatg gcgatccgcg aggacgagat cgccgcgaag
      721 gcgatgggca tcaacacccg caacctgaag ctgctcgcgt tcgggatggg cgccaccttc
      781 ggcggcatcg cgggcgcgat gttcgcgagc ttccagggtt tcatctcgcc ggagtcgttc
      841 tcgctgcagg agtcggtgat gatcgtcgcg atggtcgtgc tcggcggcat cggccacctg
      901 cccggcgtga tcgtcggcgc gctgctgctg tcggcgctgc ccgaggtgct gcgctatgtc
      961 gccgggccgc tgcagcagat gaccggtggg cggcttgatg cgtcgatcct gcggcagctg
     1021 ctgatcgcgc tggcgatgat cgtgatcatg ctgtcgcgcc cgcgtggcct gtggccgtcg
     1081 gcggagcacg gtaaggacgt gaccccggac accttgccgg tcgacccgca cgtgcgcctg
     1141 acgccggccg agcagatccc gccgccggtc tcgccgatcg atccgagcgt gaaatgagcc
     1201 ggagaatccc atgagcgaaa tcattctcaa ggtggctggt gtgtccaagc gcttcggcgg
     1261 attgcaggcg ctgtccgacg tcggcatcac gatcacgaag ggccaggtct atggcctgat
     1321 cggcccgaac ggcgccggca agacgacctt cttcaacgtg ctgaccggcc tgtacacgcc
     1381 cgactccggc agcttcgagc tcggcggcaa gccctacaag ccgacggcgg tgcacgaggt
     1441 cgcgaaggcc ggcatcgcgc gcacgttcca gaacatccgc ctgttcgccg agatgacggc
     1501 gctcgagaac gtgatggtcg gccgccatgt gcgcaccgct tccgggctgg tcggcgcggt
     1561 gctgcgcacc cggggtttca aggacgaaga ggctgcgatc gcgaagcgcg cgcaggaact
     1621 gctcgactac gtcggcatcg gccgcttcgc gccgtacaag gcgcgcacgc tgagctacgg
     1681 tgaccagcgc cggctcgaga tcgcgcgcgc gctggccacc gacccgaagc tgatcgcact
     1741 cgacgagccg gccgccggca tgaacgcgag cgagaagctc gtgctgcgcg agctgatcga
     1801 ccggatccgg cgcgacggcc gcacgatcct gctgatcgag cacgacgtga agctcgtgat
     1861 gggcttgtgc gatcgcgtcg ccgtgctcga ttacggccgc aagatcgccg agggcgcgcc
     1921 ggccagcgtc cagcgcgatg ccgctgtcat cgcggcttac ctgggcggcg cgcttgtcta
     1981 accgtcgcga ccgcgcgacc gcgcccgatc gacgggcatc gcatgcctga cgccggcaaa
     2041 gtccagccat tgctcgaagt catcggcctc aaagtcgctt atggcggcat taacgccgtc
     2101 aagggaatcg actttgcggt tgccgccggc gagatggtca cactgatcgg cgccaacggc
     2161 gcgggcaaga ccactacgtt gaaagcgctg accggcctgg tccaccccag cgcgggccgc
     2221 gtccgctaca atggcgcgga tattaccgcc ctgccgtcgc atcgcctggt cgcgctcggc
     2281 atcgcgctgg ttccggaagg ccggggcgtg tttccgcgcc tgacggtcga agaaaacatc
     2341 gacatgggcg cttattgccg tgacgacaaa cgggagatac gggccgatca tgagcatgtg
     2401 cttacgctgt tcccccggct cgctgagcgc caccagcagg cggccggcac gctgtcgggc
     2461 ggcgaacagc agatgcttgc catcgcgcgc gcgatcatgg gccggccacg gctcctgctg
     2521 ctcgatgaac cgagcatggg attggcgccc ttgatggtgc agaaaatatt cgaaaccatc
     2581 cgcgcaatcg ccgcgaccgg cgtcaccacg ctgctcgtcg agcaaaatgc caagctcgcg
     2641 c
//
LOCUS       DASRDG010000692         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1494219, whole genome shotgun sequence.
ACCESSION   DASRDG010000692 DASRDG010000000
VERSION     DASRDG010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494219"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2361)
                     /locus_tag="VFA35_11635"
     CDS             complement(<1..>2361)
                     /locus_tag="VFA35_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZT56871.1"
                     /translation="RLQPQAVEEIRAHASRNQLSLLDALLAGGYLREADLAEQMQYEL
                     EEEVYDLFFCKDARFEFHENVNKLPEREGVVDERFFLNTESVIMEAARRIDEWSYISE
                     RITSGLEVFCPTDQPYDGGQGDHDTQSVYDHVDGRRNVARIVELTSLPSFAVFKSLCQ
                     LFDGGCVVQLPEKSVVPTGKACLAAGNTQDAINLFEKAIEQQIGIPQVHGLAAEAYQS
                     VFEYESAVYHLQCSAEYKIAAHDYRGAAQQLKAATALVPTDLTSRDRLVEIALAHDLR
                     SSDFDAVAAGKELVDLFLAAGELNRVRALLERLLHARPDDLDLKKLLVNVHTKAGDQA
                     RVIELYLSIADNLVADHKPLEAVGYLQKILMLDRSRSDVSERVRSLYTLDERTRSRRR
                     TLIGLGVLSVLLFVLGLAYQWYDSRAVEDFEHIDVAAMLQAGEFEHAELEYQRFVTEH
                     PFATVNSRANEALARIGGLRLKHEAELANQRAAHDLDIQKLRQDYRTEWRRHAELFKA
                     GKAEESLAALERVAKLVHQSNQPEDHAWALEQGVENNLTRLRAFLDKATELRHRSEEE
                     LAAGHVDVARTLAIDLLTNYDIAAAAKGATIPVRFQSRPMGALILRGGAPLTATGAGG
                     QQPLRTPALVMCGREPAEFTFQLDGFEPAVARVDARQQAVVDEPLVVIPDRRIRFAAP
                     VSLLGIGSGHLVAGLRSGKVAIASLSGGQVLRLIDLGGLREMDGEPVVTGDRVFFSSN
                     EGTLECWRLDNGRAPEGWPQKLASGVATEIVARDGRLVFVDRDGQVC"
BASE COUNT          317 a          827 c          841 g          376 t
ORIGIN      
        1 acagacctgg ccgtcgcggt cgacgaacac gaggcggccg tctcgcgcga cgatctcggt
       61 cgccacgccg ctcgcgagct tctgcggcca gccttccggc gcgcggccgt tgtcgagccg
      121 ccagcactcg agggtgccct cgttgctgga gaagaacacg cgatcgccgg tcaccacggg
      181 ctcgccgtcc atctcgcgca ggccgccgag gtcgatcagg cgcagcacct ggccgccgga
      241 caacgacgcg atcgcgacct tgccgctgcg caggcccgcg acgaggtgcc cgctgccgat
      301 gccgagcagc gacaccggcg cggcgaagcg gatgcggcgg tcggggatca ccacgagggg
      361 ctcgtcgacg accgcctgct ggcgcgcgtc gaccctggcg accgcgggct cgaagccgtc
      421 gagctggaac gtgaactccg cgggctcgcg cccgcacatc accagcgccg gcgtgcgcag
      481 cggctgctgg ccgcccgcgc cggtcgccgt gagcggggcg ccgccgcgca ggatcagggc
      541 ccccatcggc cgcgactgga agcgcaccgg gatcgtcgcc cccttcgccg cggcggcgat
      601 gtcgtagttg gtcaacaggt cgatggccag cgtgcgggcc acgtcgacgt ggccggcggc
      661 gagctcctcc tcgctccggt ggcgcagctc cgtcgccttg tcgaggaacg cgcgcaggcg
      721 cgtcaggttg ttctcgaccc cctgttcgag cgcccacgcg tggtcctcgg gctggttcga
      781 ctggtgcacc agcttggcga cgcgctcgag cgccgcgagc gactcctcgg ccttgccggc
      841 cttgaacagc tccgcgtggc ggcgccactc ggtgcggtag tcctggcgca gcttctggat
      901 gtcgaggtcg tgcgccgcgc gctggttggc gagctccgct tcgtgcttga ggcgcaggcc
      961 gccgatgcgc gccagcgcct cgttggcgcg gctgttgacg gtcgcgaacg ggtgctcggt
     1021 cacgaaccgc tggtactcga gctcggcgtg ctcgaactcg cccgcctgca gcatcgccgc
     1081 gacgtcgatg tgctcgaagt cctccaccgc gcggctgtcg taccactggt atgcgaggcc
     1141 gagcacgaac agcagcaccg acagcacgcc cagcccgatc agcgtgcggc ggcggctgcg
     1201 cgtgcgctcg tcgagcgtgt agagcgacct gacgcgctcg ctcacgtcgc tgcgcgagcg
     1261 gtcgagcatc aggatcttct gcaggtagcc gacggcctcg agcggcttgt ggtcggcgac
     1321 cagattgtcg gcgatcgaca ggtagagctc gatcacgcgc gcctggtcgc cggccttggt
     1381 gtgcacgttg accagcagct tcttgagatc caggtcgtcg gggcgcgcgt gcagcaggcg
     1441 ctccagcagc gcgcgcacgc ggttcagctc gcccgcggcg aggaacaggt cgacgagctc
     1501 cttgccggcc gcgacggcgt cgaagtccga cgagcgcagg tcgtgcgcga gcgcgatctc
     1561 gaccagccgg tcgcgcgacg tgaggtcggt cggcacgagc gcggtcgcgg ccttcagctg
     1621 ctgcgcggcg ccgcggtagt cgtgcgcggc gatcttgtac tcggcgctgc actgcaggtg
     1681 gtagacggcg ctctcgtact cgaagaccga ctggtaggcc tcggcggcga gtccgtgcac
     1741 ctgcgggatg ccgatctgct gctcgatcgc cttctcgaac aggttgatcg cgtcctgcgt
     1801 gttgcccgcg gcgaggcacg ccttgcccgt cggcacgacg ctcttctcgg gcagctgcac
     1861 gacgcagccg ccgtcgaaga gctggcagag gctcttgaac acggcgaagc tcggcaggct
     1921 cgtgagctcg acgatgcgcg cgacgttgcg gcggccgtcg acgtggtcgt agacggactg
     1981 tgtgtcgtgg tcgccctggc cgccgtcgta cggctggtcc gtgggacaga acacctcgag
     2041 gccgctcgtg atgcgctccg agatgtacga ccactcgtcg atgcgacggg cggcctccat
     2101 gatcacgctc tcggtgttca ggaagaaccg ctcgtcgacg acgccttccc gctccggcag
     2161 cttgtttacg ttctcgtgga actcgaaccg cgcgtccttg cagaagaaga ggtcgtagac
     2221 ctcctcctcg agctcgtact gcatctgctc ggcgaggtcc gcctcgcgca gatacccgcc
     2281 ggcgagcaac gcgtcgagca gcgacagctg gttgcggctc gcgtgcgccc ggatctcctc
     2341 gaccgcctgc ggctgcaggc g
//
LOCUS       DASRDG010000693         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1494241, whole genome shotgun sequence.
ACCESSION   DASRDG010000693 DASRDG010000000
VERSION     DASRDG010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494241"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2460
                     /locus_tag="VFA35_11640"
     CDS             <1..>2460
                     /locus_tag="VFA35_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZT56872.1"
                     /translation="DPLEGWMEELYRRVSDRQTMGSVVQELRASLSEAEKLIDQFFRN
                     PSDRTVLIPVPNQLSAMRGVLSVLGMDQAAAALLRMRDEVDGMVSTEIDPQRVAQAGV
                     FDRLAGNLGALGFLIDMLSVQPQMAKSLFSYDAEAGTLSPVMGRGEAHAPAATRPPTA
                     AAPVDQRLIEQAQLLAFSSVREDVPVAEVSRDLERLSHEAQAADQPALAATVLRAQEA
                     LEQAFDSPADAAAVRSELSEALVDFVSTASDPVGLDPEVRPSPRGTVSAPMTLDFAPS
                     TGSAQLAQDNEMREIFLEEAREVLGHARDASAALADAPDDLNLLTTLRRAFHTLKGSS
                     RMVGLKDFGEGAWACEQLYNTQLAEQTVVPPALREFTDWSLGYLTSWVDDIAAQRHGS
                     HDAATVIEAAKLAGGVPPVPLVLESASVHGALALPPDLPSAADLDLAPAVPAPADGEL
                     PALSFELDLAGLDAPASSEHAPLDLTPTEAHEPFEALPVPDTIEGAFADPIDVDLDLD
                     LDLSGAHAAPAAPEPLTEPASEHGGLDDELVKVVGPLRIGIPLFNIYLNEADELSRRL
                     CTELAEWAMELYRPIGETPIALAHSLAGSSATVGFADLSQLARLLEHAQARSLAIGHG
                     TPEEARLFVDAADEIRRLLHQFAAGFLKEPAPELLARLAEHELSSARRLEAAAAAAEL
                     PPVSEPGALAGRPQAAPEPAPPWLEPAADDAPPQRFETSVTSPSDSIFGRESGFQPLG
                     VAELKPFGEIPVEAPRAASAVHALVDADDAIDAVDAVDAELFPIFAEEAEELLPQLAS
                     RLRDWARRPGDAALPSACMRTL"
BASE COUNT          344 a          929 c          846 g          341 t
ORIGIN      
        1 cgatccgctc gaaggctgga tggaggagct gtaccgccgc gtctccgacc gtcagacgat
       61 gggcagcgtc gtgcaggagc tgcgcgcgtc gctgtcggaa gccgagaagc tgatcgacca
      121 gttcttccgc aatccgtccg accgaaccgt gctgatcccg gtgccgaacc agctttccgc
      181 gatgcgcggc gtgctgtcgg tgctcggcat ggaccaggct gcggcggcgc tgctgcgcat
      241 gcgcgacgaa gtcgacggca tggtctcgac cgagatcgat ccgcagcgtg tcgcccaggc
      301 gggtgtgttc gaccgcctcg ccggcaacct cggggcgctc ggcttcctga tcgacatgct
      361 cagcgtgcag ccgcagatgg ccaagtcgct gttcagctac gacgccgaag ccggcacgct
      421 gagcccggtg atgggccgcg gcgaggcgca tgcgcccgcg gcgacccgcc cccccaccgc
      481 cgccgcgccg gtcgaccagc gcctgatcga acaggcgcag ctgctcgcgt tcagctcggt
      541 gcgcgaagac gtgcccgtcg ccgaggtgtc gcgcgatctg gagcgcctgt cgcacgaggc
      601 gcaggccgcc gatcagcctg cgctcgccgc cacggtgttg cgcgcgcagg aagcgctcga
      661 acaggctttc gattcgccgg ccgacgcggc ggcggtgcgc agcgaactct ccgaggcgct
      721 ggtcgacttc gtctcgaccg cgtccgaccc cgtcggcctc gatcccgagg tcaggccgag
      781 cccgcgcggc acggtcagcg cgccgatgac gctcgacttc gcgccctcga ccggcagcgc
      841 ccagctcgcg caggacaacg agatgcgcga gatcttcctc gaagaagcgc gcgaggtgct
      901 cggccatgcg cgcgacgcga gcgccgcgct cgccgacgcg cccgacgacc tgaacctgct
      961 gaccacgctg cgccgcgcct tccacacgct caagggcagc tcgcgcatgg tcgggctgaa
     1021 ggacttcggc gaaggcgcct gggcctgcga acagctctac aacacgcagc tcgccgagca
     1081 aaccgtcgtg ccgcccgcac tgcgcgagtt caccgactgg agcctgggct acctcacaag
     1141 ctgggtcgac gacatcgccg cgcaacgcca cggcagccac gatgcggcga ccgtgatcga
     1201 ggccgcgaag ctggccggcg gcgtgccgcc ggtgcctctg gtgctcgagt cggcctcggt
     1261 gcatggtgcg ctcgcgctgc cgcccgatct gccgagcgct gccgacctcg atctcgcgcc
     1321 ggccgtgccc gcgccggccg acggcgagtt gccggcgctg tcgttcgagc tcgacctcgc
     1381 cggcctcgac gcgccggcct ccagcgagca cgccccgctc gacctgacgc cgaccgaagc
     1441 gcacgagccg ttcgaggcgc tgccggtgcc cgacaccatc gagggcgcgt tcgccgaccc
     1501 gatcgatgtc gaccttgatc tcgacctcga cctgtcgggt gcgcacgccg caccggcagc
     1561 gcccgaaccg ctgaccgaac ccgcgtcgga acacggcggc ctcgacgacg agctcgtcaa
     1621 ggtcgtcggc ccgctgcgca tcggcatccc gctgttcaac atctacctga acgaggccga
     1681 cgaactgtcg cgtcggcttt gcaccgagct cgccgagtgg gcaatggagc tgtaccgccc
     1741 gatcggcgag acgccgatcg cgctggcgca ttcgctcgcg ggcagctcgg ccacggttgg
     1801 gttcgccgat ctgtcgcagc tcgcgcgcct gctcgagcat gcgcaggcac gctcgctggc
     1861 gatcggccac ggcacgcccg aagaggcgcg cctgttcgtc gatgccgccg acgagatccg
     1921 tcggctgctg caccagtttg ccgccggctt cctgaaggag ccggcgcccg agttgctggc
     1981 gcgcctggcc gaacacgaac tcagttcggc gcgccgcctc gaagcggccg ccgccgcagc
     2041 cgagctgccg ccggtctcgg agccgggcgc gctcgccggg cggccgcagg ccgcgcccga
     2101 acccgcgccg ccgtggctcg aaccggcggc cgacgacgcc ccgccgcagc gcttcgagac
     2161 cagcgtcacc tcgccatccg attcgatctt cggccgcgag agcggcttcc agcccctcgg
     2221 cgtcgccgag ttgaagccgt tcggcgagat cccggtcgag gcgccacgcg cggcctcggc
     2281 ggtgcacgcg ctggtggacg ccgacgacgc gatcgatgcg gtcgatgcgg tcgacgccga
     2341 gctgttcccg atcttcgcgg aagaggccga agaattgctg ccgcagctgg cgagccgcct
     2401 gcgcgactgg gcccgccggc ccggcgacgc cgcattgccg tcggcctgca tgcgcaccct
//
LOCUS       DASRDG010000694         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_914192, whole genome shotgun sequence.
ACCESSION   DASRDG010000694 DASRDG010000000
VERSION     DASRDG010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914192"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1792
                     /locus_tag="VFA35_11645"
     CDS             167..1792
                     /locus_tag="VFA35_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZT56873.1"
                     /translation="MIRFVKNLEIAIMALAAFGFWTGNVVVLLACVLLMGLHSTIFGP
                     VKFAYLPQHLNERELTGGNGMVEMGTFVAILLGQVAGGLLVAIPEVGRHFVAIGCVAV
                     ALLGRLTAQGVPPSPSSDPNLRINWNPVTETWRNLMLARENIVVFRSLLGISWMWFFG
                     AVFLSLFPAFAKDVLHGDERVASLLLVVFSIGIGTGSLLCELLSKRHVEIGLVPLGAI
                     GMSVFAVDLYFAARGLAPSAGYALGGFLAQPAHWRVMADLALLSLFAGLYSVPMYALI
                     QLRSQPSHRARIIAANNILNALFMIVSSLIVGALLAAHLTIPQVFLITGLLNAAVAFY
                     IFMLVPEYFLRFVAFIVTRVIYRFKVRGDENIPASGAAILVCNHVAFVDPVLLMAASP
                     RPIRFIMDHRIFAMPVLGWFFRLAKAIPIAPQRDDPVAYERAFAKAREVLNDGDLLCI
                     FPEGALTRDGELGEFKGGVMKLLESNPVPVVPLALQNLWGSYFSRQGGAAMSQPFRRG
                     WFSRVGLVAGGALAPSAVTPAGLREKVADLLAS"
     gene            1827..2507
                     /locus_tag="VFA35_11650"
     CDS             1827..2507
                     /locus_tag="VFA35_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HZT56874.1"
                     /translation="MALQLSRAAVARIVPFAVFMVLLAARGAVPADGAWGIDPRWVYG
                     VTVLVVGALLAYFWREYGELVAQTWPSLSEIVLGAGIGLAVFALWIHLDAPWMTIGAP
                     SASFLPLDAQGGLIWPLVVVRWIGAALVVPVMEELFWRSFLMRWVQSPQFESVVPQRV
                     APKAVVLSTFVFMLAHTLWLAAIVAGLAYAWLYVRTGKLWVPVIAHAVTNGALGVWVV
                     MTGNWVFW"
     gene            complement(2508..>2811)
                     /locus_tag="VFA35_11655"
     CDS             complement(2508..>2811)
                     /locus_tag="VFA35_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HZT56875.1"
                     /translation="LLGFAGEVFVLTSVYLVMPVGRLRISHALIGGVTAAVLWEITRH
                     VLIWYFATLSQVSVVYGSLTTAIVVLLSLEIAATLLLFGAQVISEYERLGREPART"
BASE COUNT          360 a          929 c         1003 g          519 t
ORIGIN      
        1 gcgcgctcga cggcgtggcc ggcaccgcct gcgagaccgc gcggcccagc accgccatca
       61 ccaggcaggt gatggccacc gcttgcgcgc ccggcagcca gctgccctcg ggcagcgcga
      121 tcatcagccc gccggccagg cgaccgacaa gtacgacaag acggcggtga tccgcttcgt
      181 caagaacctc gagatcgcga tcatggcgct cgcggcgttc ggcttctgga ccggcaacgt
      241 cgtcgtgctg ctcgcctgcg tgctgctgat gggactgcac tcgacgatct tcggcccggt
      301 caagttcgcc tacctgccgc agcacctgaa cgagcgcgag ctgaccggtg gcaacggcat
      361 ggtcgagatg ggcaccttcg tcgcgatcct gctcgggcag gtggccggtg gattgctggt
      421 cgcgatcccc gaggtcggcc ggcactttgt tgcgatcggt tgcgtcgcgg tcgcgctgct
      481 cgggcgcctg acggcgcagg gcgtgccgcc gtcgccgagc agcgacccga acctgcgcat
      541 caactggaac ccggtcaccg agacctggcg caacctgatg ctggcgcggg agaacatcgt
      601 cgtgttccgc tcgctgctgg gcatctcgtg gatgtggttc ttcggcgcgg tgttcctgtc
      661 gctgttcccg gcgttcgcga aggacgtgct gcacggcgac gagcgggtcg cgtcgctgct
      721 gctggtggtg ttctcgatcg gcatcggcac cggctcgctg ctgtgcgagc tgctgagcaa
      781 gcgccatgtc gagatcggcc tggtgccgct gggcgcgatc ggcatgagcg tgttcgcggt
      841 cgacctgtac ttcgcggcgc gcggcctcgc gccgtcggcc ggctacgcgc tgggcggctt
      901 tctcgcgcag ccggcgcatt ggcgcgtgat ggccgatctg gcgctgctgt cgctgttcgc
      961 gggcctgtac agcgtgccga tgtacgcgct gatccagctg cgctcgcagc ccagccaccg
     1021 cgcccgcatc atcgcggcga acaacatcct gaacgcgctg ttcatgatcg tcagctcgct
     1081 gatcgtcggc gcgctgctgg ccgcgcatct cacgattccg caggtgttcc tgatcaccgg
     1141 cttgctgaac gcggcggtcg cgttctacat cttcatgctg gtgcccgagt acttcctacg
     1201 cttcgtcgcg ttcatcgtca cgcgcgtgat ctaccgcttc aaggtccgtg gcgacgagaa
     1261 cattccggcc agcggcgcgg cgatcctggt gtgcaaccac gtcgcgttcg tcgacccggt
     1321 gctgctgatg gccgcgagcc cgcggccgat ccgcttcatc atggaccaca ggatcttcgc
     1381 gatgccggtg ctgggctggt tcttccgtct ggccaaggcg atcccgatcg cgccgcagcg
     1441 cgacgacccg gtcgcctatg agcgggcctt cgcgaaggcg cgcgaggtgc tgaacgacgg
     1501 cgacctgctg tgcatcttcc ccgagggcgc gctgacgcgc gacggcgaac tgggcgaatt
     1561 caagggcggc gtgatgaagc tgctcgaatc gaacccggtg ccggtggtgc cgctggcgct
     1621 gcagaacctg tggggctcgt acttttcgcg ccagggcggc gcggcgatga gccagccgtt
     1681 ccggcgcggc tggttcagcc gggtcggcct ggtcgcgggc ggtgcgctcg cgccttcggc
     1741 cgtcacgccg gcgggtcttc gcgagaaagt ggccgacctt ctggcatcct gagcgccttt
     1801 ttgcagtccg cggggaggcg acaagaatgg ccttgcagtt gagccgcgcc gcggtggcgc
     1861 gcatcgtccc gttcgccgtg ttcatggtgc tgctggccgc gcgcggcgcg gtgccggccg
     1921 acggcgcgtg gggcatcgac ccgcgctggg tctacggcgt caccgtgttg gtcgtgggcg
     1981 cgctgctcgc gtacttctgg cgcgagtatg gcgagctggt cgcgcagacc tggccctcgt
     2041 tgtcggagat cgtgctcggg gccggtatcg gcctggcggt gttcgcgctg tggatccacc
     2101 tcgatgcacc gtggatgacg atcggtgcgc cttcggcgag ctttctgccg ctcgatgcgc
     2161 aaggcgggct gatctggccg ctcgtcgtcg tgcgctggat cggcgcggcg ctggtcgtgc
     2221 cggtgatgga ggaactgttc tggcgctcgt ttctgatgcg ctgggtccag agcccgcagt
     2281 tcgagtcggt tgtgccgcag cgggtcgcgc cgaaggcggt cgtgctctcg accttcgtct
     2341 tcatgcttgc gcacacgctg tggctggccg cgatcgtcgc cggcctcgcg tatgcgtggc
     2401 tctacgtgcg caccggcaag ctgtgggtgc cggtgatcgc gcacgcggtc acgaacggcg
     2461 cgctcggggt ctgggtcgtg atgacgggga actgggtgtt ctggtgatca ggtgcgtgcg
     2521 ggttcgcgcc cgagccgctc atactccgag atgacctgcg cgccgaacag cagcagcgtc
     2581 gccgcgatct cgaggctgag cagcaccacg atcgccgtcg tcagcgaccc gtagaccacg
     2641 ctgacctggg acagtgtcgc gaagtaccag atcagcacgt ggcgcgtgat ctcccagagc
     2701 accgccgcgg tcacgccgcc gatcagcgcg tggctgatgc gcagccgccc gaccggcatc
     2761 acgaggtaga cggacgtgag gacgaacacc tcgcccgcga agccgagcag g
//
LOCUS       DASRDG010000695         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1716081, whole genome shotgun sequence.
ACCESSION   DASRDG010000695 DASRDG010000000
VERSION     DASRDG010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716081"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFA35_11660"
     CDS             <1..576
                     /locus_tag="VFA35_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HZT56876.1"
                     /translation="VDDLSLRDLLALAGGTARGEAGARPLPPRLQALLRAALDDLRRH
                     RAADGAATVAAMRSQIDALDGLLAEARERAPQIARTYGDKLQRRVNEILAEHGLQLQP
                     ADVLREIALFADRTDVTEELHRLEAHLAEARALFAQGGELGRKLEFLLQEMLREANTL
                     GAKSPDAAMAHAVVAIKSAIDKLKEQAANLE"
     gene            600..1154
                     /gene="gmk"
                     /locus_tag="VFA35_11665"
     CDS             600..1154
                     /gene="gmk"
                     /locus_tag="VFA35_11665"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZT56877.1"
                     /translation="MVVISGPSGAGKTSVCRALKERPGVEFSVSATTRPMRAGESEGV
                     DYHFLTRAEFESRVRCGEFLEWASYNDNLYGTLRAPMTQALGAGRTFLLEIEVEGTRQ
                     LRAQRVPGLYLFIAPPSLDALRERLEKRGANSRTDIDARVRIAEQEIAAAAGTVEGRP
                     LYDHTIVNDRLDATIRRVEELIGR"
     gene            1151..1705
                     /locus_tag="VFA35_11670"
     CDS             1151..1705
                     /locus_tag="VFA35_11670"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HZT56878.1"
                     /translation="MSARREILLGVGGGIAAYKVAALASRLVQAGCSVRVAMTPRALE
                     FVSALTFEGLTGRKAILSPTQIDVDGSVPHIAAAATAHAYAIAPATADLIGRLAAGLA
                     DDPVCLLALTCRCPILLCPAMNDAMWLNPVVQRNVEQLTARGMRLCGPVVGHLAEGYD
                     AIGRMAEPETIAAAILGIAAPGPS"
     gene            1730..>2062
                     /locus_tag="VFA35_11675"
     CDS             1730..>2062
                     /locus_tag="VFA35_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56879.1"
                     /translation="MRSRERLRAFRLVGRGAALALDRLHVHADVRGALEEDGAVTVWL
                     RGPLPPFARRGVRVEELPAARANATATGRERDRAIRIADDLLVRPPWVRPPRRFLGIE
                     LVVPRGMAF"
BASE COUNT          272 a          754 c          786 g          250 t
ORIGIN      
        1 gtcgacgacc tgtcgctgcg cgacctgctg gcgctcgcgg gcggcaccgc gcgcggcgag
       61 gcgggcgcgc ggccgctgcc gccgcggctg caggcgctgc tgcgcgcggc gctcgacgac
      121 ctccggcggc accgcgccgc cgacggcgcg gcgacggtcg cggcgatgcg cagccagatc
      181 gacgccctcg acggcctgct ggccgaggcg cgcgaacgcg cgccgcagat cgcgcgcacc
      241 tacggcgaca agctccagcg ccgggtcaac gagatcctcg ccgagcacgg ccttcagctg
      301 cagcccgcgg acgtgctgcg cgagatcgcc ctgttcgccg accgcaccga cgtcaccgag
      361 gagctgcacc gcctcgaagc gcacctcgcc gaggcgcgcg cgctgttcgc gcagggcggc
      421 gagctcgggc gcaagctcga gttcctgctg caggagatgc tgcgcgaggc caacaccctg
      481 ggcgccaagt cgcccgacgc ggcgatggcg cacgccgtcg tggcgatcaa gtcggcgatc
      541 gacaagctca aggagcaggc ggccaacctc gaatgaccgt cagtcgaagg atgggcagga
      601 tggtggtgat ctcgggcccg tcgggggccg gcaagacgtc ggtctgccgg gcgctcaagg
      661 agcggccggg cgtcgagttc tccgtctcgg cgacgacgcg gccgatgcgc gcgggcgaga
      721 gcgagggcgt cgactaccac ttcctcacgc gcgcggagtt cgagtcgcgc gtgcggtgcg
      781 gcgagttcct cgagtgggcg agctacaacg acaacctcta cggcacgctg cgcgcaccga
      841 tgacgcaggc actcggcgcc ggccgcacgt tcctgctcga gatcgaggtc gaggggacgc
      901 ggcagctgcg cgcgcagcgc gtgcccggcc tctacctgtt catcgcgccg ccgagcctcg
      961 acgcgctgcg cgagcgcctc gagaagcgcg gggccaacag caggaccgac atcgacgcgc
     1021 gcgtgcggat cgccgagcag gagatcgcgg cggcggcagg caccgtggaa ggccgcccgc
     1081 tctacgacca cacgatcgtc aacgaccggc tcgacgcgac catccgccgc gtcgaggagc
     1141 tgatcggccg atgagcgcgc gccgcgagat cctcctgggc gtcggcggcg gcatcgccgc
     1201 ctacaaggtc gcggcgctcg cgagccggct ggtgcaggcg ggctgctcgg tgcgcgtggc
     1261 gatgacgccg cgcgccctcg agttcgtcag cgcgctcacg ttcgaagggc tcacgggccg
     1321 caaggcgatc ctctcgccga cgcagatcga cgtcgacggc agcgtgccgc acatcgccgc
     1381 cgcagccacg gcgcacgcgt acgcgatcgc gccggcgacc gcggacctga tcggcaggct
     1441 cgcggccggc ctggccgacg acccggtgtg cctgctcgcg ctcacgtgcc ggtgcccgat
     1501 cctgctctgc ccggcgatga acgacgcgat gtggctcaac ccggtcgtgc agcgcaacgt
     1561 cgagcagctc acggcgcgcg gcatgcggct ctgcggcccg gtggtcggcc acctcgccga
     1621 gggctacgac gcgatcggcc gcatggcgga gcccgagacg atcgccgcgg cgatcctggg
     1681 gatcgcggcg cccggaccgt cgtgaggtgg tttgggtgag gtcgcgcgcg tgaggtcgcg
     1741 agagcggctg cgcgcgttcc ggctggtcgg ccgcggcgcc gcgctcgccc tcgatcggct
     1801 gcacgtgcac gccgacgtgc gcggcgcact cgaggaggac ggcgccgtga cggtctggct
     1861 gcgcgggccg ctgccgccgt tcgcgcgccg cggcgtgcgc gtcgaagaac tgccggccgc
     1921 gcgcgccaat gcgaccgcga ccgggcgcga gcgcgaccgc gcgatccgca tcgccgacga
     1981 cctgctcgtg cggccgccgt gggtgcggcc gccgcggcgc ttcctgggca tcgagctggt
     2041 cgtgccgcgc gggatggcgt tc
//
LOCUS       DASRDG010000696         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_682980, whole genome shotgun sequence.
ACCESSION   DASRDG010000696 DASRDG010000000
VERSION     DASRDG010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682980"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFA35_11680"
     CDS             complement(<1..533)
                     /locus_tag="VFA35_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZT56880.1"
                     /translation="MSATYELHGPVAVITLNNPPVNGLGHATRLGIVQGLERAQADPA
                     VKAIVITGAGKAFSGGADIKEFGSPKALAEPNLHSVIRALEASTKPIVAAIHSVVMGG
                     GLELALGCHYRVAAPGTLVALPEVKLGLLPGAGGTQRLPRVIGVENALNMIVSGEPVK
                     SELLAQAPGQKLFDKMI"
     gene            complement(657..1352)
                     /locus_tag="VFA35_11685"
     CDS             complement(657..1352)
                     /locus_tag="VFA35_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT56881.1"
                     /translation="MLQVSALNAYYGKSHILHGVDMQIREGEIVSLLGRNGSGRSTTV
                     KAIMGLVSATGSVKFKGQEMLGLQSFQVAHRGIGYVPENRDIFPKLTVHQNLLLGEKR
                     GKTKPRWSYEDMYKMFPRLKEREHTEAGVMSGGEQQMLTLCRTLMGDPDLIMIDEPTE
                     GLAPKIVELVAEYLKELKRRGIAVLLVEQKLTIALEISDRCCVMGHGSIVFEGTPAEL
                     RANADIRKEWLEV"
     gene            complement(1374..2153)
                     /locus_tag="VFA35_11690"
     CDS             complement(1374..2153)
                     /locus_tag="VFA35_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZT56882.1"
                     /translation="MSKYAVELKDLRKAFGKTEIIRGSNLAVTAGERVAIIGPNGAGK
                     STLFNLISGRFAPTSGEILLNGQRIGGKEPFEINRLGLARSFQVSNLFTRLSVFENLR
                     CGVLWSLGYRYSFWRFLGSAHDANARADELVKKIRLEHKRDELAMNLTYAEQRALEIG
                     VTIASGADVVLLDEPTAGMSKSETKRFTELMREVTVGKTLLTVEHDMGVVFGLADKIA
                     VLVYGEVIAFDTPEAVRANPRVQEAYLGSTLADAQAAEAGH"
     gene            complement(2150..>2628)
                     /locus_tag="VFA35_11695"
     CDS             complement(2150..>2628)
                     /locus_tag="VFA35_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56883.1"
                     /translation="SEITKAWLLYLGLIFLFMVMFAPGGVAGLIMTNLRVAAHGKLGR
                     LLPSYAAVGLAGAVAVVGIAALVEMIYHKQLGDGASTTLNYLGYTLDTAATGSWLTAL
                     AVTVVGVLLFIPARKRFAQQWGDVQGEIVREAHTRSDDALDATAAVGGDATTGEAA"
BASE COUNT          396 a          908 c          857 g          467 t
ORIGIN      
        1 tcgatcatct tgtcgaacag cttctgtccc ggcgcctgcg ccagcagttc gctcttcacc
       61 ggctcgccgc tgacgatcat gttcagcgcg ttctcgaccc cgatcacgcg cggcaggcgt
      121 tgcgtgccgc cggcgcccgg cagcaggccg agcttgactt cgggcagcgc caccagcgtg
      181 ccgggcgcgg cgacgcggta gtggcagccc agcgcgagct cgagcccgcc gcccatgacc
      241 acgctgtgga tcgcggcgac gatcggcttg gtgctggctt ccagcgcgcg gatcacgctg
      301 tgcaggttcg gctcggccag cgccttcggc gagccgaact ccttgatgtc ggcgccgccc
      361 gagaaggcct tgccggcgcc ggtgatcacg atcgccttca cggccgggtc ggcctgcgcc
      421 cgctcgagcc cctgcacgat ccccagccgc gtcgcgtggc cgaggccgtt gacgggcggg
      481 ttgttcagcg tgatgacggc caccgggcca tgaagttcgt aggtcgcgct catgcttcgt
      541 atcctcggga gttcggcaga aatagaacga tcgttcgatt ctaggtcggg aacgaaaaag
      601 ggcgctgtct caccagtgac agcgccctcg ggggtcggat cgtgacgcgc tggcggctac
      661 acctccaacc actccttgcg gatgtcagcg ttggcgcgca gctccgcggg agtgccctcg
      721 aagacgatgc tgccgtggcc catgacgcag cagcggtcgg agatctccag cgcgatggtg
      781 agcttctgct cgaccagcag caccgcgatg ccgcggcgct tgagctcctt caggtactcg
      841 gccacgagct ccacgatctt cggtgccagc ccctcggtgg gctcgtcgat catgatgaga
      901 tcggggtcgc ccatgagcgt gcggcacagc gtgagcatct gctgctcgcc gccggacatc
      961 acgcccgcct cggtgtgctc gcgctccttc agtcgcggga acatcttgta catgtcctcg
     1021 taggaccagc gcggcttggt cttgccgcgc ttctcgccca gcagcaggtt ctggtgcacc
     1081 gtgagcttgg gaaagatgtc gcggttctcc ggcacatagc cgatgccgcg gtgcgccacc
     1141 tggaacgact gcaggccgag catctcctgg cccttgaact tcaccgagcc ggtggccgac
     1201 accagcccca tgatggcctt gacggtggtg gagcggcccg agccgttgcg accgagcagg
     1261 ctcacgatct cgccttcgcg gatctgcatg tccacgccat gcaggatgtg gctcttgccg
     1321 tagtaggcgt tcagagcact gacttgcagc atgcctcgct ctccctcttg ttgtcagtgc
     1381 ccggcttcgg cggcctgcgc gtcggccagc gtcgagccca ggtaagcctc ttgcacgcgc
     1441 ggattcgcgc gcaccgcctc cggcgtgtcg aaggcgatca cctcgccata gaccagcacc
     1501 gcaatcttgt cggccagacc gaacaccacg cccatgtcgt gctcgacggt gagcagcgtc
     1561 ttgccgaccg tgacctcgcg catcagctcg gtgaagcgct tggtttcgct cttgctcatg
     1621 ccggcggtgg gctcgtcgag caggaccacg tccgcaccgc tggcgatcgt gacgccgatc
     1681 tcgagcgcac gctgctcggc gtaggtgagg ttcatcgcca gctcgtcgcg cttgtgctcg
     1741 aggcggatct tcttgaccag ctcgtcggcg cgcgcgttcg cgtcgtgcgc cgagccgagg
     1801 aagcgccaga atgaatagcg gtagccgagc gaccacagca cgccgcagcg caggttctcg
     1861 aacaccgaca gccgggtgaa caggttgctc acctggaagc tgcgcgccag gcccaggcgg
     1921 ttgatctcga acggctcctt gccgccgatg cgctggccgt tgagcaggat ctcgccgctg
     1981 gtgggcgcga agcggccgct gatcagattg aacagcgtcg acttgccggc gccgttcggc
     2041 ccgatgatcg cgacccgctc gccggcggtg accgccaggt tggagccgcg gatgatctcc
     2101 gtcttgccga aggccttgcg caagtccttc agttcgactg cgtatttgct catgccgcct
     2161 ccccggtcgt tgcgtcgccg cccacagcgg cggtcgcatc cagcgcgtcg tcgctgcgcg
     2221 tatgcgcctc gcggacaatt tcaccctgca cgtcgcccca ctgctgcgcg aagcgtttgc
     2281 gcgccggtat gaagagcagc acgcccacca cggtgacggc cagcgcggtc aaccagctgc
     2341 cggtggcggc ggtatcgagc gtgtaaccca ggtagttcag cgtcgtgctc gcaccgtcgc
     2401 cgagctgctt gtggtagatc atctcgacca gggccgcgat gcccaccacc gccaccgcgc
     2461 cggccaggcc cacggccgca tagctcggca gcagccggcc cagcttgccg tgcgcggcaa
     2521 cgcgcaggtt ggtcatgatc aggcccgcca cgccgccggg tgcgaacatc accatgaaca
     2581 ggaagatcag gccgaggtag agcagccagg ccttggtgat ctccgaca
//
LOCUS       DASRDG010000697         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1372644, whole genome shotgun sequence.
ACCESSION   DASRDG010000697 DASRDG010000000
VERSION     DASRDG010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372644"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2532)
                     /locus_tag="VFA35_11700"
     CDS             complement(<1..2532)
                     /locus_tag="VFA35_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZT56884.1"
                     /translation="MLLTRKSSGSGRAPVSSLVASLERGVGRAIPTMDRRSFLRRSGL
                     GVGVGLATSQLSLVRKAKAADAPAGGDAKPVVRRTVCSHCSVGCAVDAVVENGVWVRQ
                     EPVFDSPLNLGAHCAKGAALREHGHGDYRLKTPMKLVDGKYQKISWDQALNEISAKML
                     ELKKASGPDSIFVVGSSKHNNEQSYLLRKWVSLWGSNNCDHQARICHSTTVAGVANTW
                     GYGAMTNSYNDMRNSKCAMYIGSNAAEAHPVSMLHMLHAKETGTKMIVVDPRFTRTAA
                     KADEYVRLRSGSDIAFLFGMMHHIFKNGWEDKKYIADRVYGMDKVRDEALSKWTPDKV
                     QEVSGVDEATVYKIAKMMADNRPSTVVWCMGQTQHTTGNAIVRASCLLQLALGNVGVS
                     GGGTNIFRGHDNVQGATDVGPNPDSLPGYYGLAEGSWKHFAKVWGVDFEWIKKQYAPG
                     MMGKPGMTVSRWIDGVLEKNELIDQDSNLRGLIFWGHAPNSQTRGLEMKTAMDKLDLM
                     VVIDPYPSASAAMAAMPGKAGELNANRAVYLLPAATQFECAGSVTASNRSLQWREKVI
                     EPLFESRTDHMIMYQLAQKLGFDKELVAKTKMVAGKGGMMEPEVESILREINQSCWSI
                     GYTGQSPERLKAHMRNMNVFDVKTLRAKGGVDKETGYKLDGDYFGLPWPCFGTPEMKH
                     PGSPNLYDTSRHVMDGGGNFRANFGVERDGVDLLAADGSYSKGADITTGYPEFDHVLM
                     KKLGWWNELTDVEKAAAEGKNWKTDLSGGIIRVTMKNHGCHPFGNAKARAVVWNFPDG
                     VPKHREPLYGIRSDLIADYPTHDDKKAFWRLPTLYKSVQDKNKDIG"
     gene            complement(2557..>2792)
                     /locus_tag="VFA35_11705"
     CDS             complement(2557..>2792)
                     /locus_tag="VFA35_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56885.1"
                     /translation="MKPSTPKPIPPLAEQPPRVARRGLLVGAGVAGAAAVAAGGLHRA
                     MLAEVGAAAPAAAEGAGYRLTPHVLRYYETTKA"
BASE COUNT          400 a          924 c          944 g          524 t
ORIGIN      
        1 gccgatgtcc ttgttcttgt cctgcaccga cttgtacaag gtcggcaggc gccagaacgc
       61 cttcttgtcg tcgtgggtgg ggtagtcggc aatgagatcc gagcggatgc cgtacagggg
      121 ctcgcggtgc ttcggcactc cgtccgggaa gttccacacc accgcgcgcg ccttggcgtt
      181 gccgaacgga tggcagccgt ggttcttcat ggtcacccgg atgatcccgc cggagagatc
      241 ggtcttccag ttcttgcctt cggcggccgc cttctccacg tcggtgagct cgttccacca
      301 accgagcttc ttcatcagca cgtggtcgaa ctcgggatag ccggtggtga tgtcggcgcc
      361 cttcgaatac gagccgtcgg cggcgagcag atcgacgccg tccctttcca ccccgaagtt
      421 ggcgcggaag ttgccgccgc cgtccatcac gtggcggctg gtgtcgtaga ggttcggcga
      481 tccggggtgc ttcatctccg gcgtgccgaa gcacggccac ggcaggccga agtagtcgcc
      541 gtcgagcttg tagccggtct ccttgtcgac gccgcccttg gcacgcagcg tcttcacgtc
      601 gaagacgttc atgttgcgca tgtgcgcctt cagccgctcg ggcgactgcc cggtgtagcc
      661 gatcgaccag cagctctggt tgatctcgcg caggatcgac tcgacctcgg gctccatcat
      721 cccgcccttg ccggcgacca tcttggtctt ggccaccagc tccttgtcga agccgagctt
      781 ttgcgccagc tggtacatga tcatgtgatc ggtgcgcgac tcgaacagcg gctcgatcac
      841 cttctcgcgc cattgcagcg agcggttcga cgccgtcacc gagccggcgc actcgaactg
      901 cgtcgccgcc ggcagcaggt agacggcgcg gttggcgttg agctcgccgg ccttgcccgg
      961 catcgccgcc atcgccgccg aggccgacgg atacggatcg atgacgacca tcaggtcgag
     1021 cttgtccatc gccgtcttca tctcgaggcc gcgggtctgc gagttcggcg catggcccca
     1081 gaagatcagg ccgcgcaggt tcgaatcctg gtcgatcagc tcgttcttct cgagcacgcc
     1141 gtcgatccag cgcgacaccg tcatgccggg cttgcccatc atgccgggtg cgtactgctt
     1201 cttgatccac tcgaagtcga cgccccagac cttggcgaag tgcttccagg aaccctcggc
     1261 cagaccgtag tagccgggca gcgagtcggg gttcgggccg acgtcggtcg cgccctgcac
     1321 gttgtcgtgg ccgcggaaga tgttggtgcc gccgccgctg acgccgacgt tgccgagcgc
     1381 gagctgcagg aggcaggagg cgcggacaat ggcgttgccg gtggtgtgct gggtctggcc
     1441 catgcaccag acaacggtgc tcggccggtt gtcggccatc atcttcgcga tcttgtagac
     1501 cgtggcttcg tcgacgccgc tcacttcctg gaccttgtcg ggcgtccact tgctgagcgc
     1561 ctcgtcgcgc accttgtcca tgccgtagac gcgatcggcg atgtacttct tgtcttccca
     1621 gccgttcttg aagatgtgat gcatcatccc gaacaggaag gcaatgtcgc tgccggagcg
     1681 cagccgcacg tactcgtcgg ccttggccgc ggtgcgggtg aagcgcggat cgacgacgat
     1741 catcttcgtg ccggtctcct tcgcgtgcag catgtgcagc atcgagaccg ggtgcgcctc
     1801 ggcggcgttc gaaccgatgt acatcgcgca cttcgagttg cgcatgtcgt tgtacgagtt
     1861 cgtcattgcg ccgtagcccc acgtgttcgc gacgcccgcg accgtggtgg agtggcagat
     1921 gcgcgcctgg tgatcgcagt tgttgctgcc ccacaggctc acccacttgc gcagcaggta
     1981 ggactgctcg ttgttgtgct tcgacgagcc gacgacgaag atcgagtccg gaccgctcgc
     2041 cttcttcagc tcgagcatct tggcgctgat ctcgttcagc gcctgatccc agctgatctt
     2101 ctggtacttg ccgtcgacca gcttcatcgg cgtcttcagg cgatagtcgc cgtggccgtg
     2161 ctcgcgcagg gcggcgccct tggcgcagtg ggcgccgagg ttgagcggcg agtcgaacac
     2221 cggctcctgg cgcacccaga cgccgttctc gaccaccgca tcgacggcgc agccgaccga
     2281 acagtggctg cagacggtgc ggcggacgac cggcttggcg tcgccgccgg ccggtgcgtc
     2341 ggcggccttg gccttcctca ccaggctgag ctgcgaggtc gccaggccga cgccgacgcc
     2401 gaggccggag cggcgcagga aggagcgccg gtccatggtc gggatggcgc ggccgacgcc
     2461 acgctccagg ctggcgacca gcgacgaaac cggagcgcgg ccgctcccgg aggatttgcg
     2521 cgtgagcaac atcgggatct cccgtcgacg ggccggtcag gccttcgtgg tttcgtagta
     2581 gcgcaggacg tgcggcgtga ggcggtagcc cgcgccttcg gcggccgccg gggcggcggc
     2641 gccgacttcg gcaagcatgg cgcggtgcag gccacccgcc gcgacggcag cggcgccggc
     2701 aacgccggcg ccgaccagga ggccgcgacg ggcgacacgc gggggctgtt cggccagcgg
     2761 cgggatcggc ttgggggtag aaggcttcat cg
//
LOCUS       DASRDG010000698         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_566213, whole genome shotgun sequence.
ACCESSION   DASRDG010000698 DASRDG010000000
VERSION     DASRDG010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566213"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="VFA35_11710"
     CDS             complement(<1..1455)
                     /locus_tag="VFA35_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56886.1"
                     /translation="MQAPAASRTGPLEPWLWFLLAFGVYAACGATVFYKTDGPDILWL
                     LHEGLPHPWHVGYLPLLKAWRWVIHLVGIEPSLMRLGTSFSALGAAVGVAGFAAGLRR
                     LGLDAPRARAAVLLLAAAPTALLFATCVEFHALLLGATGVAFWWTTVMIARPSWWGMA
                     LLGALTHVAFLMHFSGICLPAFLLSFFVARRWADSAARGRTLALGAVAGVVHVVLWYA
                     LPRLFVAFYGVHSDLAAAFAKEASIGRPQSLDWTPVIFAQEWLWPVLPLSVTCWLGAT
                     RRGLRLEFAAFVLGFLPFLYLCVRQLVFEPENGAYLLPLVPAAALLTVQALPLRVAAS
                     LALVAVLGAPFVVRCEQTGWDDPAEYARVIAGYDGAAAGRPYVVLLGTHREMSIGYAR
                     LAPWTGAAPRRDFLYVRQMATMPRTLYTEAMRRGSVEGLRRVIDAGGAVLTTAATRAF
                     LRDPESVMRAEKLLADIPRNDEMTGPLLSADLERA"
     gene            complement(1553..>2102)
                     /locus_tag="VFA35_11715"
     CDS             complement(1553..>2102)
                     /locus_tag="VFA35_11715"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56887.1"
                     /translation="VVHLFDVREPVSQVDKALAAYCAEHHKPVVLVGNKVDLAPEVDL
                     PQWDRYMRQQLPGIDHAPIAFLSAKDGTNVEPVVDLLFELQQQARTSMTTGRLNTVLH
                     AARDKLLPRDRGQFPKLFYGTQIGTEPITVLVFVNEPKLFDPPYERYLAQVLRDAFGC
                     PEVPIRLVFRRRAKVVLDSREG"
BASE COUNT          342 a          771 c          761 g          228 t
ORIGIN      
        1 cgcgcgctcg aggtcggcgc tcagcagcgg gccggtcatc tcgtcgttgc gcggaatgtc
       61 ggcgagcagc ttctccgcgc gcatcaccga ctccggatcg cgcaggaacg cgcgggtcgc
      121 cgcggtcgtc agcacggcgc cgccggcgtc gatcacgcgc cggagccctt cgaccgaacc
      181 gcgccgcatc gcctccgtgt acagcgtgcg cggcatcgtc gccatctgcc gcacgtagag
      241 gaagtcgcgg cgcggcgcgg cgccggtcca cggcgcaagc cgcgcgtagc cgatcgacat
      301 ctcgcggtgc gtgccgagca gcaccacgta cgggcggccg gccgccgcgc cgtcgtagcc
      361 ggcgatcacg cgcgcgtact cggccggatc gtcccagccg gtctgctcgc agcgcacgac
      421 gaacggcgcg ccgagcacgg cgacgagcgc gagcgacgcc gccacgcgga gcggcagcgc
      481 ctgcaccgtc agcagcgcgg cggcgggcac cagcggcagc aggtaggcgc cgttctcggg
      541 ctcgaacacg agctggcgca cgcagaggta gaggaacggc aggaagccga gcacgaacgc
      601 cgcaaactcg agccgcagtc cgcgccgcgt cgcaccgagc cagcaggtca ccgacagcgg
      661 cagcaccggc cacaaccatt cctgcgcgaa gatcacgggc gtccagtcga gcgactgcgg
      721 gcggccgatc gacgcctcct tcgcgaacgc ggcggcgagg tcgctgtgca cgccgtagaa
      781 ggcgacgaac agccgcggca gcgcgtacca cagcaccacg tgcacgacgc ccgcgaccgc
      841 gccgagcgcc agcgtgcggc cgcgtgcggc actgtcggcc cagcgccgcg cgacgaagaa
      901 cgacagcaag aaggccggca ggcagatgcc cgagaagtgc atcaggaacg cgacgtgcgt
      961 cagcgcgccg agcaacgcca tcccccacca gctcggccgc gcgatcatca ccgtggtcca
     1021 ccagaacgcg acgccggtgg cgccgagcag cagcgcgtgg aactcgacgc aggtggcgaa
     1081 cagcagcgcg gtcggggccg ccgccagcag cagcacggcg gcgcgggcgc gcggcgcgtc
     1141 gagccccagc cgccgcaggc cggcggcgaa cccggcgacg ccgaccgccg cgccgagcgc
     1201 cgagaacgaa gtgccgagcc gcatcagcga cggctcgatg cccaccagat ggatcaccca
     1261 ccgccacgcc ttcagcagcg gcaggtagcc gacgtgccac ggatgcggca gcccctcgtg
     1321 caagagccag aggatgtcgg gcccgtcggt cttgtagaag accgtggcgc cgcacgccgc
     1381 gtagacgccg aacgcgagca ggaaccagag ccacggctcc agcgggcccg ttcgcgacgc
     1441 cgcgggcgct tgcatcgacg gcgcacggtg ccgccgccgc ccagtatcgt caagcgcgtc
     1501 gtcgagggcg ccgtcgagcg cgccgtcgag cgcgccgggg ccgcgcgcgc gatcagcctt
     1561 cgcggctgtc gagcaccacc ttcgcacggc ggcggaacac gaggcggatc ggcacctcgg
     1621 ggcagccgaa ggcgtcgcgc agcacctgcg cgaggtagcg ttcgtacggg gggtcgaaca
     1681 gcttcggctc gttgacgaac accagcacgg tgatcggctc ggtgccgatc tgcgtcccgt
     1741 agaacagctt cgggaactgg ccgcggtctc gcggcagcag cttgtcgcgc gccgcgtgca
     1801 gcacggtgtt gagccggccc gtcgtcatcg acgtgcgcgc ctgctgctgc agctcgaaca
     1861 gcaggtcgac gaccggctcg acgttggtgc cgtccttcgc cgacaggaac gcgatcggcg
     1921 cgtggtcgat gccgggcagc tgctggcgca tgtagcggtc ccactgcggc aggtcgacct
     1981 cgggcgcgag gtcgaccttg ttgccgacca gcacgaccgg cttgtggtgc tcggcgcagt
     2041 acgcggcgag cgccttgtcg acctggctca ccggctcgcg cacgtcgaac aggtgcacga
     2101 cg
//
LOCUS       DASRDG010000699         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_112213, whole genome shotgun sequence.
ACCESSION   DASRDG010000699 DASRDG010000000
VERSION     DASRDG010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112213"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFA35_11720"
     CDS             complement(<1..648)
                     /locus_tag="VFA35_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZT56888.1"
                     /translation="MAKLFLRTFGCQMNEYDSGRIADLLRAAEGLERAARPEDADVIV
                     FNTCSVRDKAQEKVFADLGRVRHLKQANPALMIAVGGCVASQEGPALVKRAPFVDVVF
                     GPQTLHRLPELLARRRASGEPQVDVSFPEIEKFDHLPPARVEGATAFVSIMEGCSKYC
                     TFCVVPYTRGDEVSRGFDSVMDEVRSLASQGVGEIHLLGQNVNAYAGAMNDGTVVD"
     gene            complement(649..957)
                     /locus_tag="VFA35_11725"
     CDS             complement(649..957)
                     /locus_tag="VFA35_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56889.1"
                     /translation="MRILLVVLSAAYCAAAAAQLRTIPDEAKRGVMSHIEVMTVNLNG
                     QAVELAAGVLIRDAENRIIVPVSLPPDSVVKYQLDAESRIRRVWVLTPDEAAQADKQQ
                     "
     gene            complement(970..1046)
                     /locus_tag="VFA35_11730"
     tRNA            complement(970..1046)
                     /locus_tag="VFA35_11730"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1010..1012),aa:Met,seq:cat)
     gene            1047..2219
                     /locus_tag="VFA35_11735"
     CDS             1047..2219
                     /locus_tag="VFA35_11735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HZT56890.1"
                     /translation="MSIDAAIINRDRDLIVVGGGPVGACLATAAKGLRVALVSQERRA
                     AGAPTPREFDARVYALTPGNVEFLRGLGVWRHVPAARVQPVLAMRVFGDDGRSSLEFD
                     AYRAGVPELAWIVEDATLQDAFWQVLDAEVIVADCERLEVEGDAVLALRDGRRISAAL
                     VVGADGANSFVRRAAGIAATEQGYAQSAVVANFRCEKPHDGVAWQWFQGGPVLALLPL
                     PDQQVSMVWSLPEARAQAVARLPGEALCREVESASRRMLGALSLTTPPRHYALRRLRA
                     QRLVQPRVALVGDAAHVIHPLAGQGLNLGLQDAQTLARVLVAREPGRDPGDARLLRRY
                     ERSRAEPILAMNAVVDGLFRLFGSEGEGWSRLRNAGLNLTDRLPVLKNILIRQAMN"
     gene            2219..>2608
                     /locus_tag="VFA35_11740"
     CDS             2219..>2608
                     /locus_tag="VFA35_11740"
                     /inference="COORDINATES: protein motif:HMM:NF021896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide isomerase DsbC N-terminal
                     domain-containing protein"
                     /protein_id="HZT56891.1"
                     /translation="MRTFWMLVLTAVFAMPAFGNEATIRKVLEPKLGGAKIEGVERGP
                     AGLWEVRYRGEEGVRILYTDATASYIIVGKIYESKADRDLTEERLRKLNAINFSSLPL
                     SQAVKVQRGNGKRVLVMFSDPYCPYCKQ"
BASE COUNT          396 a          876 c          903 g          433 t
ORIGIN      
        1 gtcgacaacg gtgccgtcat tcatcgcgcc cgcataggcg ttgacgttct ggcctaacaa
       61 atggatctcg cccacaccct gcgaagctag cgagcggact tcatccatca ccgaatcgaa
      121 gccgcggctc acttcatcgc cgcgcgtgta cggcaccacg cagaacgtgc agtacttgct
      181 gcagccttcc atgatcgaga cgaatgccgt agctccctcg acccgtgcgg gcggcaggtg
      241 atcgaacttc tcgatctccg gaaagctgac gtcgacctgc ggctcgccgc tggcgcgccg
      301 ccgcgcgagc agctcgggga ggcggtgcag cgtctgcggc ccgaacacga cgtcgacgaa
      361 cggcgcgcgc ttcacgagcg ccgggccttc ctggctcgcg acgcagccgc cgaccgcgat
      421 catcagcgcc ggattggcct gcttcaggtg ccgcacgcgg ccgaggtcgg cgaacacttt
      481 ctcctgcgcc ttgtcgcgca ccgagcaggt gttgaacacg atcacgtcgg catcctcggg
      541 acgcgccgcg cgctcgaggc cttcggcggc gcgcagcagg tcggcgatgc gtcccgagtc
      601 gtactcgttc atctggcagc cgaaggtgcg caggaagagc ttggccattt actgctgctt
      661 gtccgcctgc gcggcctcgt ccggcgtgag gacccagacg cggcgaatcc tgctttccgc
      721 gtcgagctgg tacttcacga cgctatccgg cggcagcgac accggcacga tgatgcgatt
      781 ctccgcgtcg cggatcagca cgccggctgc gagctccacc gcctggccgt tgaggttgac
      841 ggtcatgacc tcgatatggc tcatcacgcc gcgcttcgcc tcgtcgggaa tggtgcgcag
      901 ctgcgcggcg gcagcggcgc agtacgcggc ggacaggacg acgagcagga ttctcatcgc
      961 gggaggaatt ggtggcgaat ccgtgattcg aacacgggac acgcggatta tgattccgct
     1021 gctctaacca gctgagctaa ttcgccatgt cgattgatgc cgccattata aaccgggacc
     1081 gcgacctgat cgtcgtcggc ggcgggccgg tgggcgcctg cctcgcgact gccgcgaagg
     1141 gcttgcgcgt tgccctggtc tcccaggaaa gacgcgccgc gggggctccg acgccccgcg
     1201 agttcgacgc gcgcgtctat gcgctgacgc cgggcaatgt cgagttcctg cgaggcctcg
     1261 gcgtctggcg ccacgtcccc gccgcgcgcg tccagccggt gctcgcgatg cgggtgttcg
     1321 gcgacgatgg gcggtcgagc ctcgaattcg atgcctaccg cgccggcgtg ccggagctcg
     1381 cctggatcgt cgaggacgcg acattgcagg atgccttctg gcaggtgctc gacgccgaag
     1441 tcatcgtcgc ggactgcgag cgactggaag tcgaaggcga cgccgtgctc gcgctgcgcg
     1501 acgggcgacg catctcggca gccctcgtgg tcggcgccga cggcgcgaac tcgttcgtgc
     1561 gccgcgctgc ggggatcgcg gcgaccgagc agggttatgc gcagtccgcg gtggtggcga
     1621 actttcgctg cgagaagccg cacgacggcg tggcctggca gtggttccag ggcggcccgg
     1681 tgctcgcgct gctgccgctt cccgatcagc aggtgtcgat ggtgtggtcg ttgcccgaag
     1741 cgcgcgccca ggccgtcgcc aggctgccgg gcgaggcgct gtgtcgcgag gtcgaatcgg
     1801 cgtcgcggcg catgctcggt gcgctctcgc tgacgacgcc gccccgccac tacgcgctgc
     1861 gcaggctgcg cgcgcagcga ctcgtgcagc cgcgcgtcgc gctggtcggc gatgcggcgc
     1921 acgtcatcca tccgctcgcc ggccagggac tgaacctcgg cctgcaggac gcgcagacgc
     1981 tcgctcgagt gctcgtcgcg cgcgagcccg gtcgcgaccc cggcgatgcg cgcctgctgc
     2041 gccggtatga gcgcagccgc gcagagccta tactggcgat gaatgccgtg gtcgacgggc
     2101 tgttccgtct cttcggcagc gaaggcgagg gctggtcacg gctgcgcaac gccgggttga
     2161 acctgaccga ccgcttgccg gtcctaaaga acattctcat acggcaggcg atgaactgat
     2221 gcgcaccttc tggatgctgg ttctgaccgc ggtcttcgcc atgccggcgt tcggcaacga
     2281 ggcgacgatc cgcaaggtgc tcgagccgaa gctcggcggc gccaagatcg agggcgtcga
     2341 gcgcgggccg gccggcctct gggaagtgcg ctaccgcggc gaggagggcg tgcgcatcct
     2401 ctacacggac gccaccgcgt cgtacatcat cgtcggcaag atctacgagt cgaaggccga
     2461 ccgggacctg accgaggagc ggctgcgcaa gctgaacgcg atcaacttca gttctctgcc
     2521 gctctcgcaa gcggtcaagg tgcagcgcgg caacggcaag agggtgctgg tgatgttctc
     2581 ggatccttac tgcccctact gcaagcag
//
LOCUS       DASRDG010000700         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1144441, whole genome shotgun sequence.
ACCESSION   DASRDG010000700 DASRDG010000000
VERSION     DASRDG010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144441"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFA35_11745"
     CDS             <1..402
                     /locus_tag="VFA35_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT56892.1"
                     /translation="AVLVFLTGPLGVAGAAAALLVLNRPFGFVALLGVIALMGMIMRN
                     SVILIDQIEQDRARGVPTWDAIVEAAVRRFRPIILTAAAAVLAMIPLSRSVFWGPMAV
                     AIMGGLIVATVLTLLSLPAMYAAWFRVKKPG"
     gene            478..564
                     /locus_tag="VFA35_11750"
     tRNA            478..564
                     /locus_tag="VFA35_11750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:512..514,aa:Leu,seq:tag)
     gene            595..1902
                     /gene="tig"
                     /locus_tag="VFA35_11755"
     CDS             595..1902
                     /gene="tig"
                     /locus_tag="VFA35_11755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZT56893.1"
                     /translation="MTVQVETLDKLERRITLTLPTATITTEVESRLKKLSRTVKADGF
                     RPGKVPMSVVAQRYGYSVHYEVMNDKVGQVFSEAVNEAKLRVAGAPKISEKEGAPEGE
                     VAFDAVFEVYPEVKLGDLSATQVERVSTEVTEAAIDKTVEILRKQRRSFAQRPAAEGA
                     VEGDRVTIDFEGKIDGVPFEGGRAEGFQFLIGEGQMLEQFDLAVRGMKSGESKTFPLQ
                     FPADYQGKEVAGKEADFMVTLKKIEAQHLPEVNEAFAKSLGIADSTVEGLRADVKKNL
                     EREVKFRVLARNKASVMDALVAASELDVPKALVAGEAERMVEAARADLKKRGLKDADT
                     APIASEIFQPQAEKRVRLGLVVGELVRANHLLARPDQLQKHIEEMAQSYEKPSEVVRW
                     YLGDQQRLAEVEAVVVENNVVEFVLGKAQVTDKVLPFEELMAG"
     gene            2036..>2323
                     /locus_tag="VFA35_11760"
     CDS             2036..>2323
                     /locus_tag="VFA35_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZT56894.1"
                     /translation="MSALETTGLGMVPMVIETSGRGERAYDIYSRMLRERVIFLVGPV
                     NDQTANLVVAQLLFLESENPDKDISLYINSPGGSVSAGLSIFDTMQFIKPDV"
BASE COUNT          414 a          730 c          807 g          372 t
ORIGIN      
        1 gccgtgctgg tgttcctgac cggtccgctg ggcgtggcgg gcgccgccgc ggcgctgctg
       61 gtgctcaacc ggccgttcgg cttcgtggcg ctgctgggcg tgatcgcgct gatgggcatg
      121 atcatgcgca actcggtgat cctgatcgac cagatcgagc aggatcgcgc ccgcggcgtg
      181 ccgacctggg acgcgattgt cgaggccgcg gtgcggcgct tccggccgat catcctgacc
      241 gcggcggcgg cggtgctggc gatgatcccg ctgtcgcgca gcgtgttctg ggggccgatg
      301 gcggtggcga tcatgggcgg gctgatcgtc gcgacggtgc tgacgctgct ctccctgccg
      361 gcgatgtacg ccgcctggtt ccgggtgaag aagccgggct agaatctgcg gttcttcaag
      421 taaccgagaa ggggttgctt caggcagccc cgccttcgtc tcctgccgaa ggcgttcgcg
      481 cgggtggcga aattggtaga cgcaccaggt ttaggtcctg acgccagcga tggtgtgcgg
      541 gttcgagtcc cgccccgcgc accagcgaca cgaaaatcga aagtactcct catcatgacc
      601 gtccaagttg aaaccctcga caagctggag cgccgcatca cgctgacgct gcccaccgcc
      661 acgatcacca ccgaagtcga gtcgcgcctg aagaagctct cgcgcaccgt caaggccgac
      721 ggctttcgcc cgggcaaggt gccgatgagc gtggtcgcgc agcgctacgg ctattcggtg
      781 cactacgagg tgatgaacga caaggtcggc caggtgttca gcgaggccgt caacgaggcc
      841 aagctgcgcg tcgccggcgc gccgaagatc tccgagaagg aaggcgcgcc cgaaggcgag
      901 gtcgcgttcg atgcggtctt cgaggtctac cccgaggtca agctcggcga tctgagcgcg
      961 acgcaagtcg aacgcgtctc gaccgaagtg accgaagcgg cgatcgacaa gaccgtcgag
     1021 atcctgcgca agcagcgccg cagcttcgcg cagcgcccgg ccgccgaagg cgccgtcgag
     1081 ggcgaccgcg tgacgatcga cttcgagggc aagatcgacg gcgtgccgtt cgagggcggc
     1141 agggcggagg gcttccagtt cctgatcggc gaaggccaga tgctcgagca gttcgacctg
     1201 gccgtgcgcg gcatgaagtc gggcgagtcg aagacctttc cgctgcagtt cccggccgac
     1261 taccagggca aggaggtcgc cggcaaggag gccgacttca tggtcacgct gaagaagatc
     1321 gaggcccagc acctgccgga agtgaacgag gcgttcgcga agtcgctcgg catcgcggat
     1381 tcgaccgtcg aaggcctgcg cgccgacgtc aagaagaacc tcgagcgcga agtcaagttc
     1441 cgcgtgctcg cgcgcaacaa ggccagcgtg atggacgcgc tggtcgccgc ctcggagctc
     1501 gacgtgccga aggcgctggt tgccggcgaa gccgagcgca tggtcgaagc cgcgcgcgcc
     1561 gacctgaaaa agcgcggctt gaaagatgcc gacaccgcgc cgatcgcgtc cgagatcttc
     1621 cagccgcaag ccgagaagcg cgtgcgcctc ggtctcgtgg tcggcgaact ggtgcgcgcg
     1681 aatcacctgc tggcccggcc cgatcagctg cagaaacaca tcgaggaaat ggcgcaaagc
     1741 tacgagaagc cgtcggaagt ggtgcgctgg tacctcggcg accagcagcg cctggccgag
     1801 gtcgaggcgg tcgtggtcga gaacaacgtc gtcgagttcg tgctcggcaa ggcccaggtc
     1861 accgacaagg tgctgccgtt cgaggagctg atggcgggct gaccggcagc gctgcagcgc
     1921 gagggcgccg ccgcgaggtg ggcgcccttt ttcctgcacg gggcgaatct tcgcggcccg
     1981 acttgtggcg acataatcga ccccaacatc cgcagtatcc atttgaagga gtgccatgag
     2041 cgcgcttgaa accaccggcc tgggcatggt tcccatggtc atcgaaacct cgggccgcgg
     2101 cgagcgggcc tacgacattt acagccgcat gctgcgcgag cgggtgatct tcctggtcgg
     2161 cccggtgaat gaccagacgg cgaacctggt cgtcgcgcaa ttgctgtttc tcgagtccga
     2221 gaacccggac aaagacatct cgctctacat caactcgccc gggggctcgg tcagcgcggg
     2281 cctgtcgatc ttcgacacga tgcagttcat caagcccgat gtg
//
LOCUS       DASRDG010000701         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1264113, whole genome shotgun sequence.
ACCESSION   DASRDG010000701 DASRDG010000000
VERSION     DASRDG010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264113"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1497)
                     /gene="tilS"
                     /locus_tag="VFA35_11765"
     CDS             complement(190..1497)
                     /gene="tilS"
                     /locus_tag="VFA35_11765"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZT56895.1"
                     /translation="MKQRRRIAVAYSGGRDSTALLHATLAAAGEQGVEVLALHVHHGL
                     SPHADAWAAHCAAQCRRWARARKPVTFVSHRVAERPAKGESVEAWARQARYRALREMA
                     LAHGVSVVLLAQHRRDQAETLLLQALRGAGVAGLAGMPRSAERDGIRWQRPWLDKPRA
                     EIDAYVRRHRLKHIEDDSNADPRFARNRLRLQVWPALTAAFEHAEASLAASAAWAQQA
                     DALLEEVAALDLAAVATDDGLQLAAWLALSAARRSNALRAWIRQRHGAGAPASLIVRL
                     LDELPDRRSASWPLFDAALRLHRGVLRYQAATSAPWPSPAPEAALGIRRAGNYALPGW
                     GGTLRAERVGEGGVPLAWLARLELRRRSGGERFQAGLGRPPRSLKKQFQAAGVPMWDR
                     DGPLVYSGGQLVFVPGLGLDARVIGLPGQSLVALRWLPLAGTV"
     gene            complement(1512..2483)
                     /locus_tag="VFA35_11770"
     CDS             complement(1512..2483)
                     /locus_tag="VFA35_11770"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZT56896.1"
                     /translation="MSKRHFLEFEQPVADLESKIEELRYVQNESAVDISEEIDRLGTK
                     SLQLTKDIYSSLTPWQVTQIARHPQRPYTLDYVSEIFTDFQELHGDRAFADDQSIVGG
                     LARFNGQACMVLGHQKGRDTKERGLRNFGMSRPEGYRKALRLMKLAEKFGLPVFTFVD
                     TPGAYPGIGAEERGQSEAIGRNIFEMAQLEVPIIVTIIGEGGSGGALAISVGDQLLML
                     QYSIYSVISPEGCASILWKTAERAADAADALGITAHRLKALGLVDKIINEPVGGAHRD
                     AKQMAAFLKRALADALRQVSDLTPKELLARRYERVQSYGRFSDTKER"
     gene            complement(2525..>2753)
                     /locus_tag="VFA35_11775"
     CDS             complement(2525..>2753)
                     /locus_tag="VFA35_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZT56897.1"
                     /translation="IGRWTAEMFLIFHLMRPNVLPLDDVGLINGISINYFSGEPVSRA
                     EAREVGDAWAPFRSVATWYIWRSLDPLPVDY"
BASE COUNT          405 a          968 c          959 g          421 t
ORIGIN      
        1 ccatcgaggt gccgccgtat ttgtggactg tgagagccat gtgaggtgat tcaggtcacg
       61 gatgtcgacg cgaggtttca acgcgaggtt tcaacgcgag gtttcaacga gtaccgcctt
      121 ggccatcggc cgcgctttcg cgctcgggcg agcccgcgat tctaacttcc gcgccgcggc
      181 ggcccggctt caaacggtgc cggccagcgg cagccagcgc aacgcgacca gcgactggcc
      241 cggcaggccg atcacgcgcg cgtcgagtcc gaggccgggg acgaacacga gctggccgcc
      301 gctgtagacg agcgggccgt cgcggtccca catcggcacg ccggcggcct ggaactgctt
      361 cttcaggctg cgcggcggcc ggccgaggcc ggcctggaaa cgctcgccgc cgcttcgccg
      421 gcgcagttcg agccgggcca gccatgcgag cgggacgcca ccctcgccca cccgctcggc
      481 ccgcagcgtg ccgccccagc cgggcagcgc gtagttgccg gcgcggcgga tgcccagggc
      541 cgcctccggc gcgggcgatg gccacggtgc gctcgtcgcc gcctgatagc gcagcacgcc
      601 gcgatgcaag cgcaacgcgg catcgaagag cggccagctc gcactgcgcc ggtcgggcag
      661 ttcgtcgagc agccgcacga tcaggctcgc cggcgcgccg gcgccgtgcc gctgccggat
      721 ccaggcgcgc agcgcgttgc tgcgccgcgc cgcggacaag gccagccacg cggcgagctg
      781 caggccgtca tcggtcgcga cggccgcgag gtcgagcgcg gcgacttctt cgagcagcgc
      841 atcggcctgc tgcgcccacg ccgccgacgc cgccagcgac gcctcggcat gctcgaacgc
      901 ggcggtcagc gccggccaca cctgcagccg caagcggttg cgcgcaaagc gcgggtcggc
      961 attgctgtcg tcctcgatgt gcttcaggcg gtggcggcgc acataggcat cgatctcggc
     1021 gcgcggcttg tcgagccagg gccgctgcca gcggatgccg tcgcgctcgg cgctgcgcgg
     1081 catgcccgcg agccccgcga cgccggcgcc gcgcagcgct tgcagcagca gggtttcggc
     1141 ctgatcgcgc cggtgctgcg cgagcagaac gaccgacacg ccgtgcgcca gcgccatctc
     1201 gcgcaacgcg cggtagcgcg cttgccgcgc ccaggcttcg acgctctcgc ctttcgcggg
     1261 acgctcggcg acgcgatgcg agacgaaggt gaccggcttg cgggcacgcg cccaacgccg
     1321 gcactgcgcc gcgcagtgcg cggcccacgc gtccgcatgc gggctcaggc catggtgcac
     1381 atgcagcgcg aggacctcga caccctgctc gccggccgcc gcgagtgtcg catgcagcaa
     1441 cgccgtcgaa tcgcgcccgc cgctgtaggc gacggcgatt cgacggcgtt gcttcacgga
     1501 aggggcaatg ctcagcgttc tttggtgtcg ctgaagcggc cgtaggactg cacgcgctcg
     1561 taccggcgcg ccagcaattc cttcggcgtc aggtcgctga cctggcgcag cgcgtcggcc
     1621 agcgcgcgct tcaggaacgc ggccatctgc ttggcatcgc ggtgcgcgcc gccgaccggc
     1681 tcgttgatga tcttgtcgac cagcccgagc gccttcaggc ggtgcgcggt gatgccgagc
     1741 gcatcggcgg catcggccgc acgctcggcg gtcttccaca ggatcgacgc gcagccctcc
     1801 ggcgagatca ccgagtagat cgagtactgc agcatcagca actggtcgcc cacgctgatc
     1861 gccagcgcgc cgcccgagcc gccctcgccg atgatggtga cgatgatcgg cacctcgagc
     1921 tgcgccatct cgaagatgtt gcgtccgatc gcctccgatt gcccgcgttc ctcggcaccg
     1981 atgcccggat aggcgcccgg cgtgtcgacg aaggtgaaga cgggcaagcc gaacttctcg
     2041 gccagcttca tcagccgcag cgccttgcga tagccctcgg ggcgcgacat gccgaagttg
     2101 cgcaggccgc gctccttcgt gtcgcggcct ttctggtgcc cgagcaccat gcaggcctgg
     2161 ccgttgaagc gggccagccc gccgacaatg gactggtcgt ccgcgaacgc acggtcgccg
     2221 tgcagttcct ggaagtcggt gaagatctcg ctgacatagt cgagcgtgta gggccgctgc
     2281 gggtggcgcg cgatctgcgt cacctgccag ggcgtcaggc tcgagtagat gtccttcgtg
     2341 agctgcagac tcttggtgcc gaggcgatcg atctcttccg agatgtcgac cgccgactcg
     2401 ttctgcacat agcgcagttc ttcgatcttc gattcgagat cggcaaccgg ctgctcgaac
     2461 tcgaggaagt ggcgtttgct cattcgtgta ttccttcttc gtgggaaggc ggctgccgcg
     2521 atgttcagta gtctaccggc agcgggtcga ggctgcgcca gatgtaccag gtcgcaaccg
     2581 agcggaacgg cgcccacgcg tcgccgacct cgcgggcctc ggcgcgcgag accggctcgc
     2641 cgctgaagta gttgatgctg atgccgttga tcaggccgac gtcgtcgagc ggcagcacgt
     2701 tcggacgcat cagatggaag atcagaaaca tctcggcagt ccagcggccg atg
//
LOCUS       DASRDG010000702         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_566835, whole genome shotgun sequence.
ACCESSION   DASRDG010000702 DASRDG010000000
VERSION     DASRDG010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566835"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VFA35_11780"
     CDS             <1..719
                     /locus_tag="VFA35_11780"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HZT56898.1"
                     /translation="FIMVPDTPDVEDVLFGASGVARGLKPGKIVVDMSSISPLATKDF
                     AKRVNDLGCQYLDAPVSGGEVGAKAASLTIMVGGSGDTFAKVKPLFDLMGKNVTLVGG
                     NGDGQTCKVANQIIVALNIAAVGEALVFAAKAGADPAKVRQALMGGFAASRILEVHGE
                     RMIKRTFAPGFRIGLHQKDLSLALAGARALGVALPQTAGAAQLMQVCAANGMQDLDHS
                     ALVKALELMADHPVAPDPAG"
     gene            878..2191
                     /locus_tag="VFA35_11785"
     CDS             878..2191
                     /locus_tag="VFA35_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZT56899.1"
                     /translation="MSTPSPTQDPRGFLRALFDAAVAQAVPGAGIEAFLPPPPKGRTL
                     VLGAGKAGGAMAAAVDAMWPKGAPISGLVVTRYDYVPPAYKALQQQGAGRIEVVEAAH
                     PVPDEAGRRAAQRIAELTRGLTADDLVLCLISGGASALLATPAEGVTLEEKQAINKAL
                     LKSGAAIDEMNCVRKHLSAIKGGRLAAMCAPAQVVTLLISDVPGDAPEVIGSGPTVPD
                     STTCADALRILARYGLAIPAAARAGLESGAFETPKPGDARFAGHRVHMIATPRQSLDA
                     AARCARDAGIAVHVLSDEMEGESREVGKVHAAMARYVARHGQPFAKPCVILSGGETTV
                     TVKSKGGRGGRATEFLLGCAIALQGEPGVHVLAGDTDGIDGVEDNAGAIVTPSTLARA
                     SGAGLKAQDFLDNNDAYNFFKPLGDLLTTGPTFTNVNDFRAVLVV"
BASE COUNT          387 a          823 c          853 g          337 t
ORIGIN      
        1 tcttcatcat ggtgccggac acgcctgacg tcgaggacgt gctcttcggc gcgagcggcg
       61 tggcccgtgg cctcaagccg ggcaagatcg tcgtcgacat gagctcgatc tcgccgctcg
      121 ccaccaagga tttcgcgaag cgcgtgaacg acctcggctg ccaatacctc gatgcgccgg
      181 tttccggcgg cgaagtcggc gcaaaggccg cctcgctcac gatcatggtc ggcggctccg
      241 gcgacacgtt tgcgaaggtc aagccgctct tcgacctcat gggcaagaac gtcacgctgg
      301 tgggcggcaa cggcgacggc cagacctgca aggtcgcgaa ccagatcatc gtcgcgctga
      361 acatcgcggc ggtcggcgag gcgctggtgt tcgcagcgaa ggccggcgcc gacccggcca
      421 aggtgcggca ggcgctgatg ggcggcttcg cggcctcacg catcctcgag gtgcacggcg
      481 agcgcatgat caagcgcacc ttcgcgccgg gctttcgcat cggcctgcac cagaaggacc
      541 tgagcctcgc gctcgccggc gcacgcgcgc tcggcgtcgc gctgccgcag acggcgggcg
      601 ccgcgcagct gatgcaggtg tgcgcggcga atggcatgca ggacctcgat cactcggcgc
      661 tcgtcaaggc gctcgagttg atggccgacc atccggtggc gccggacccg gctgggtgac
      721 gggcggaaag acagaacagc cgaccgcaaa ggcgcaaagg aacgcaaagg gcgcaaagaa
      781 gagacatcaa ggtatttcct tggcgtcttt gcgttccttt gcgcctttgc gtccaagaaa
      841 cctccccatt ccaccgtcct cccggaaacc gaaatccatg agcacgcctt cgcccacgca
      901 agacccgcgc ggcttcctgc gcgcgctgtt cgacgccgcg gtcgcgcagg ccgtgcccgg
      961 cgccggcatc gaggcgttcc tgccgccacc gccgaagggc cgcacgctgg tgctcggcgc
     1021 gggcaaggcg ggcggcgcga tggccgcggc cgtcgatgcg atgtggccga agggcgcgcc
     1081 gatctcgggg ctggtggtca cgcgctacga ctatgtgccg ccggcgtaca aggccttgca
     1141 acagcagggc gccggccgca tcgaggtcgt cgaggccgcc catccggtgc ccgacgaggc
     1201 gggtcggcgc gccgcgcagc gcatcgccga actgacccgc ggcctgacgg cggacgacct
     1261 cgtgctgtgc ctgatctccg gcggcgcctc ggcgctgctc gcgacgccgg ccgaaggcgt
     1321 gacgctcgaa gagaagcagg ccatcaacaa ggcgctgctc aagagcggcg ccgcgatcga
     1381 cgagatgaac tgcgtgcgca agcacctctc ggcgatcaag ggcggccggc tggcggcgat
     1441 gtgcgcgccc gcgcaggtcg tcacgctgct gatcagcgac gtgcccggcg atgcgcccga
     1501 ggtcatcggc agcggcccga ccgtgcccga cagcaccacc tgcgccgacg cgctgcgcat
     1561 cctcgcgcgc tacggcctcg cgatcccggc cgccgcgcgc gccgggctgg agagcggcgc
     1621 gttcgagacg cccaagcccg gcgatgcgcg cttcgcgggc caccgggtcc acatgatcgc
     1681 gacgccgcgc cagtcgctcg atgccgcggc gcggtgcgca cgcgacgccg gcatcgcggt
     1741 gcatgtgctg agcgacgaaa tggaaggcga gtcgcgcgag gtcggcaagg tgcacgcggc
     1801 catggcgcgc tacgtcgcgc ggcacggcca gccgttcgcg aagccgtgcg tgatcctgtc
     1861 gggcggcgag acgacggtga ccgtcaagag caagggcggc cgtgggggcc gcgcgaccga
     1921 gttcctgctc ggctgcgcga tcgcgctgca gggcgaaccc ggcgtgcacg tgctcgccgg
     1981 cgacaccgac ggcatcgacg gcgtcgaaga caacgccggc gcgatcgtca cgcccagcac
     2041 gctcgcccgc gcgagcggtg cgggcctgaa ggcgcaggac tttctcgaca acaacgacgc
     2101 ctacaacttc ttcaagccgc tcggcgacct gctgacgacc gggccgacgt tcacgaacgt
     2161 gaatgacttt cgggcggtgt tggtggtgtg acttgtggag cgacgagtgc gcccggcgcc
     2221 tccaccgtga aatttctctg ctgatctctg actggctcac ctggtgagct agcaggcacc
     2281 ctaagatgct ctcaccgcaa tgggaggtga ggcatggatg atcgggcgaa tcggcaccag
     2341 tcggcgcggg ctcgtttcca aagcattggg ccagcagcgg gcgcaagggt gggtcccgca
//
LOCUS       DASRDG010000703         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_684442, whole genome shotgun sequence.
ACCESSION   DASRDG010000703 DASRDG010000000
VERSION     DASRDG010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684442"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFA35_11790"
     CDS             <1..967
                     /locus_tag="VFA35_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT56900.1"
                     /translation="RVVVTGGSGSSVVATLVDVANKHNQRNPDKAILVLNHSSIDPDL
                     TGKNCSFWHFQFEANTAMKMKALAKYIKTQPDIHNAFLFNQDYAHGRQWASYGKQLVS
                     LARPDIKWVGEDLIPIGRVKDFAPYLAKIKAAGADTLITGNWGPDLTLLLRAAGDAGA
                     NLRYFNHSAGGVPGTVVAVSLAKVGRLTWVAEWHPGEQGVPKAEALAQAYKAKTGKDF
                     LAPRIDLAPRMLAAAIDKAQSTDTVKVAKALEDLSYDSVVGPVRMRAEDHQLLLPQVV
                     NTIAPVDGKAVKTGWEGTNYGFRTDAVYTGNELAQGSDCKMQRPQ"
     gene            983..1777
                     /locus_tag="VFA35_11795"
     CDS             983..1777
                     /locus_tag="VFA35_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZT56901.1"
                     /translation="MNAMNLHDTPPSPLLLALESRSVLEMAAFFSAWPWLRQAPRGDG
                     HPVLVLPGLTAGDWSTQALRAYLAAQGYVPYGWGQGPNRGPRPGVEAKMRAQLDEIAQ
                     RHGRKVSLIGWSLGGLYARELAWDATDHVRAVITLGSMFAGGPNATNVRPVYEALSGR
                     RIEDLPARRPLPPPVPSTAIYSRSDGIVAWEGCREQPSPTTENIEVHASHSGLGHHPA
                     ALYAIADRLAQPEGHWQPFRRDGLRAYLYPDPDRPAQARLGSAQPA"
     gene            complement(1794..3347)
                     /gene="gshA"
                     /locus_tag="VFA35_11800"
     CDS             complement(1794..3347)
                     /gene="gshA"
                     /locus_tag="VFA35_11800"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZT56902.1"
                     /translation="MSGLDQRLRELPDARLREMRRGVEKESLRALPDGGLALTPHPAA
                     LGSALTHPHITTDFSESQLEFVTGVHPSAQACVDELTQIHQFSYRALRELGDEMLWVS
                     SMPCGLPTDETIPIGRYGSSNVGRAKSVYRVGLGHRYGRRMQTISGIHYNWSMPGITT
                     DAYFGLIRNFRRESFLLLYLFGASPALCSSFVAGREHELQKIGSGSLHMPHGTSLRMG
                     RLGYQSDAQATLSVSYNSLESYAASLQEALTKPYPAYEKIGIRNPGGDYNQLATSLLQ
                     IENEFYSTIRPKRVIFPGERPLHALRERGVEYVEVRCMDLDPFEPVGINARTMQFIDM
                     FLLRCLLTESPPDTPDEIAALARNQQRVAARGREPGLRLERFDAAVTLTEWGAEIIAS
                     LAPIAERLDALHGGTGYRDALAAAHAVLRDPETAPSTRVLATMARDFGNSYVSYTRAQ
                     SLQTRNALLALPFGDDLRRRFADASTASVDEQKRIEANDSLPFEIYRQRYLSPERLGV
                     PGKREPARV"
BASE COUNT          551 a         1229 c         1185 g          493 t
ORIGIN      
        1 gcgcgtcgtc gtcaccggcg gctcgggctc gtcggtggtc gccacgctcg tcgacgtggc
       61 caacaagcac aaccagcgga atccggacaa ggcgatcctg gtgctgaacc actcgtcgat
      121 cgatcccgac ctgacgggca agaactgcag cttctggcac ttccagttcg aggccaacac
      181 ggcgatgaag atgaaggcgc tggccaagta catcaagacc cagcccgaca tccacaacgc
      241 cttcttgttc aaccaggact atgcgcacgg ccgccaatgg gcgagctacg gcaagcagct
      301 ggtctcgctg gcgcgccccg acatcaaatg ggtcggcgaa gacctgatcc cgatcggccg
      361 ggtcaaggat ttcgctccgt acctcgcgaa gatcaaggcc gccggcgccg acacgctgat
      421 caccggcaac tggggccccg acctgacgct gctgctgcgc gccgcgggcg atgccggtgc
      481 gaacctgcgc tacttcaacc acagcgccgg cggcgtgccg ggcaccgtgg tcgcggtgtc
      541 gctggccaag gtcggacgcc tcacctgggt cgccgaatgg catccgggcg agcaaggggt
      601 gcccaaggcc gaggcgctgg cgcaggccta caaggccaag accggcaagg acttcctcgc
      661 gccgcgcatc gacctcgcgc cacgcatgct cgccgcggcc atcgacaagg cgcagtcgac
      721 cgacaccgtg aaggtcgcca aggcactcga ggatctcagc tacgactcgg tggtgggccc
      781 ggtgcgcatg cgcgccgagg accaccaact gctgctgccg caggtcgtca acacgatcgc
      841 gccggtcgac ggcaaggcgg tcaagaccgg ctgggaaggc acgaactacg gctttcgcac
      901 cgatgccgtc tacaccggca acgaactcgc ccagggcagc gactgcaaga tgcagcgccc
      961 gcaataagca accaacgaac ccatgaacgc catgaacctg cacgacaccc cgccctcgcc
     1021 cctgctgctg gccctcgaaa gccgcagcgt gctcgaaatg gccgccttct tcagcgcctg
     1081 gccctggctg cgccaggcgc cgcgcggcga cggccacccg gtgctggtgc tgccggggct
     1141 gacggccggc gactggtcga cgcaggcact gcgggcctac ctcgcggcgc agggctacgt
     1201 gccctacggc tggggccagg ggccgaaccg cgggccgcgt cccggggtcg aggcgaagat
     1261 gcgcgcgcag ctcgacgaga tcgcgcagcg ccacggccgc aaggtcagcc tgatcggctg
     1321 gagcctcggc ggcctgtatg cgcgcgagct ggcgtgggac gcgaccgacc acgtgcgcgc
     1381 cgtgatcacg ctcggcagca tgttcgccgg cggaccgaac gcgaccaacg tgcgcccggt
     1441 gtacgaggcg ctgtccgggc gccgcatcga ggacctcccg gcgcgccggc ctctgccgcc
     1501 gccggtgccg agcaccgcga tctacagccg cagcgacggc atcgtcgcct gggagggctg
     1561 ccgcgagcag ccgtcgccga cgaccgagaa catcgaggtc cacgccagcc acagcggcct
     1621 gggccaccac ccggccgcgc tctacgccat cgccgaccgg ctggctcagc cggagggcca
     1681 ctggcagccg ttccggcgcg acggcctgcg cgcctacctc tatcccgatc ccgaccgccc
     1741 ggcgcaggcc cggctcggca gcgcgcagcc cgcgtgatcg acttcgacgc tgtctagacg
     1801 cgcgccggct cgcgcttgcc cggcacgccg agccgctcgg gcgacaggta gcgctggcgg
     1861 tagatctcga acggcaggct gtcgttggcc tcgatgcgct tttgctcgtc gaccgaggcg
     1921 gtcgaggcgt cggcgaagcg cctgcgcaag tcgtcgccga acggcagcgc gagcagcgcg
     1981 ttgcgcgtct gcagcgactg ggcgcgcgtg tagctcacgt aggagttgcc gaaatcgcgc
     2041 gccatcgtcg cgagcacgcg cgtggacggc gccgtctcgg ggtcgcgcag caccgcgtgc
     2101 gccgcggcca gcgcatcgcg atatcccgtt ccgccgtgca gcgcatccag ccgctccgcg
     2161 atcggcgcga gcgacgcgat gatctctgcg ccccactcgg tgagcgtcac cgccgcgtcg
     2221 aagcgctcga gccgcaggcc cggctcgcga ccgcgcgcgg ccacgcgctg ctggttgcgc
     2281 gcgagggccg cgatctcgtc gggcgtatcc ggcgggctct cagtgagcag gcagcgcagc
     2341 aggaacatgt cgatgaactg catcgtccgc gcgttgatgc cgaccggctc gaacgggtcg
     2401 aggtccatgc agcgcacttc gacgtactcg acgccgcgct cgcgcagcgc gtgcagcggc
     2461 cgctcgcccg ggaagatcac gcgcttggga cggatggtgc tgtagaactc gttctcgatc
     2521 tgcagcaggc tggtcgcgag ctggttgtag tcgccgccgg ggttgcggat gccgatcttc
     2581 tcgtaggcgg gatagggctt ggtcaacgcc tcctgcagcg acgcggcata actctccagg
     2641 ctgttgtagc tgaccgagag cgtcgcctgc gcgtcgctct ggtagcccag ccgccccatc
     2701 cgcagcgaag tgccgtgcgg catgtgcagg ctgccgctgc cgatcttctg cagctcgtgc
     2761 tcgcgcccgg cgacgaagct cgagcacagt gccggcgacg cgccgaacag gtacagcagc
     2821 aggaacgact cgcgcctgaa gttgcggatc agcccgaagt acgcgtcggt cgtgatgccc
     2881 ggcatcgacc agttgtagtg gatgcccgag atcgtctgca tgcgccggcc atagcgatgc
     2941 cccagcccca cgcggtaaac gctcttcgcg cggcccacgt tcgacgaacc gtagcgcccg
     3001 atcgggatcg tctcgtcggt cggcagcccg cagggcatgc tcgagaccca cagcatctcg
     3061 tcgcccagct cgcgcaacgc gcggtagctg aactgatgga tctgcgtcaa ctcgtcgaca
     3121 caggcctgcg cgctcgggtg cacgccggtg acgaattcga gctgcgattc gctgaagtcg
     3181 gtcgtgatgt gcgggtgcgt cagcgccgag ccgagcgcgg ccgggtgcgg cgtgagcgcg
     3241 aggccgccat cgggcagcgc gcgcaggctt tccttctcga cgccacggcg catctcgcgc
     3301 aggcgcgcat cgggcaattc gcgcaggcgt tggtcaagac cgctcatgcg gcgtctcctc
     3361 gaaactctct tggggcggac ggtaacagac cttgaatttg cctgccggcg cagggccagc
     3421 cgcgatcggg gctgcccttg agcgaagccg tcccggcg
//
LOCUS       DASRDG010000704         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_226920, whole genome shotgun sequence.
ACCESSION   DASRDG010000704 DASRDG010000000
VERSION     DASRDG010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226920"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFA35_11805"
     CDS             complement(<1..391)
                     /locus_tag="VFA35_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZT56903.1"
                     /translation="MIRQLQNQRDIAAARDDDPNFAGTLAKGLMILQSFVRDPRPRAN
                     SELAATLGLSRPTVSRLCRTLVELGYLDRDEHIDRYFSGPMAVALGYPYIVSVPVRAQ
                     ARPAMQALANEVRGAASIGVAIGLDVVY"
     gene            732..1238
                     /locus_tag="VFA35_11810"
     CDS             732..1238
                     /locus_tag="VFA35_11810"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HZT56904.1"
                     /translation="MKSRSWFKLGAACLTILLAPSAPAATATGSLTVSATVVSACSVS
                     AATLNFGSSIDLTSAALPLDASSTMTVLCTSTTPYSIALDAGANAGGATNFGARAIKN
                     GSHTLGYQLYLDAPRTMIWGNGTGSSTSPGVGIGSNQTLTIYGRLPGLTGAVPGTYTD
                     TVTVTISY"
     gene            1254..1976
                     /locus_tag="VFA35_11815"
     CDS             1254..1976
                     /locus_tag="VFA35_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HZT56905.1"
                     /translation="MRALFRIALCLVLAHAAAAAASEIGVTPVVVHLDKAHDRATLSV
                     VNSGAEPVIMQAEVIEWKRSNGAEEDAPSADMVINPSVFTVQPGQAQLVRVGLRRAAP
                     AQREGTYRIVLREVPAAPRAGELRISGQVQVLMALRVPIYVAPHAVVRELRWQARREP
                     DGSVTATLHNEGNVHARVGRMQLRSSEGVLASQAFPAVVFPGETQSFRWHGLGQIVAQ
                     PLTLEAATDHGAHSVPVALAGR"
     gene            1993..>2439
                     /locus_tag="VFA35_11820"
     CDS             1993..>2439
                     /locus_tag="VFA35_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56906.1"
                     /translation="MHRSVATAACAMLLMANAEAAAAVGTPRNSAPEAARPGASATAE
                     LLGVRLNDVEQPDAVLVYREPTGSLAVPVAALAQWRVRRPDAPVLRLDGHDCVALDAI
                     PGARWRVDAARQLLVLDLPPAAFERHQFEMSGPAQPAQTGASLGGFV"
BASE COUNT          359 a          866 c          856 g          358 t
ORIGIN      
        1 tgtacaccac gtccagcccg atcgccacgc cgatggaagc tgcaccccgc acctcgttgg
       61 ccagcgcctg catggctggc ctggcctgcg cgcgcacggg cacggagacg atgtacggat
      121 agccgagcgc gacggccatc ggcccgctga agtagcggtc gatgtgctcg tcgcggtcga
      181 gatagccgag ttccaccagc gtgcggcaca ggcgcgacac cgtcgggcgc gacaggccga
      241 gcgtcgccgc cagttcgctg ttggcgcgcg gccgcgggtc gcgcacgaag ctctgcagga
      301 tcatcagccc cttggccagg gtccccgcga aattgggatc gtcgtcgcgc gcggcggcga
      361 tgtcgcgctg gttctgcagt tggcggatca tggcgatggc ccaggcgggc ttgtctcgtg
      421 tgtagtaggt tgcccagcgc cgccaacgcc gggcgaggac tggtgcgcac tctagtcccc
      481 gggcaggccg cctcaaagcg gcgtttcatt attcaaaaca actgttcaat ctgccggcca
      541 gggtcacagc ttccggcatc agcggccgct gctgtcggcg caggcgtacc gcgtgatgca
      601 actcacgctg gctgtgccgg ggggtgatgg ggcgctcaac ttcgcccgca acctgccgtc
      661 aagcatgacg tgcggagctc gaccgcagcg acctgaatcg acattccaac tgatcgagcc
      721 aaggactgcg catgaaatcc cgctcctggt tcaaactcgg tgccgcctgc ctgacgatcc
      781 tgctggcgcc gtccgccccc gcggcgacgg cgaccggcag cctgacggtg tcggccaccg
      841 tggtcagcgc gtgctcggta tctgccgcca cgctcaactt cggcagcagc atcgacctca
      901 cctcggcggc cttgccgctc gacgccagct ccacgatgac ggtgctgtgc acctcgacca
      961 cgccctacag catcgcgctc gatgcgggcg cgaatgccgg cggcgccacc aacttcggcg
     1021 cgcgtgcgat caagaacggc agccacacgc tgggctacca gctctacctc gacgcgccgc
     1081 gcacgatgat ctggggcaac ggcaccggca gcagcacctc gcccggcgtg ggcatcggct
     1141 cgaaccagac cttgacgatc tatgggcgcc tgccggggct caccggtgcc gttcccggca
     1201 cctacaccga cacggtgacg gtgacgattt cctactgaaa gcagggatcg accatgcgcg
     1261 ccctgtttcg gattgccttg tgcctggtgc tggcccacgc ggccgcagcg gcggccagcg
     1321 agatcggcgt gacgccggtg gtggtgcacc tggacaaggc ccacgaccgg gccacgctga
     1381 gcgtcgtcaa cagtggcgcc gagccggtga tcatgcaggc cgaggtgatc gagtggaagc
     1441 gcagcaacgg cgccgaggaa gacgccccga gcgccgacat ggtcatcaac ccatcggtct
     1501 tcacggttca gccgggccag gcgcagctgg tccgcgtcgg cctgcgccgc gccgcgccgg
     1561 cgcagcgcga gggtacctac cggatcgtgc tgcgcgaggt gccggccgcg ccgcgggccg
     1621 gcgagctgcg gatcagcggc caggtgcagg ttctgatggc gctgcgcgtg ccgatctacg
     1681 tggcgccgca cgccgtcgtg cgcgagctgc gctggcaggc ccggcgcgag cccgatggca
     1741 gcgtcaccgc cacgctgcac aacgagggca atgtgcatgc gcgcgtcggc cgcatgcagc
     1801 ttcgttcgag cgaaggcgtg ctcgcgagcc aagccttccc ggcggtggtg tttccgggcg
     1861 agacgcaaag cttccgctgg cacgggctcg ggcagatcgt cgctcagccg ctgacgctgg
     1921 aagccgccac cgaccacggc gcgcacagcg tgccggtcgc gctggccggg cgttgaacgc
     1981 cgcggcaccg ccatgcaccg gtccgtggcg accgccgcat gcgcgatgct gttgatggcg
     2041 aacgccgagg ccgcggcggc cgtcggcacg ccgcgcaact cggcgccgga ggccgcgcga
     2101 cccggcgcca gcgcaacggc ggaactgctg ggcgtgcggc tcaacgatgt cgagcagccc
     2161 gatgcggtgc tggtgtaccg cgagccgacg ggcagcctgg cggttccggt cgccgcactc
     2221 gcgcagtggc gcgtgcgccg acccgatgcg ccggtgctgc gcctcgacgg ccacgattgt
     2281 gtcgcgctcg acgcgatccc cggcgcgcgc tggcgcgtcg acgccgcccg ccagttgctg
     2341 gtgctggact tgccgcctgc cgcgttcgag cgccatcaat tcgagatgtc ggggccggcg
     2401 cagccggcac agaccggcgc cagcctcggc ggcttcgtc
//
LOCUS       DASRDG010000705         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_916562, whole genome shotgun sequence.
ACCESSION   DASRDG010000705 DASRDG010000000
VERSION     DASRDG010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916562"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFA35_11825"
     CDS             <1..591
                     /locus_tag="VFA35_11825"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZT56907.1"
                     /translation="PDIDALLPQVDFAGSSPAQLLPSGPGFQHATTHDLIVALLYGAQ
                     ERVVLATPYFVPDDAFLQALAAAVLRGAEVHLVVPERGNNRFVDLAGPSWFEYLLEAG
                     VHVHLHQPRFLHSKHVSFDRTIALVGSSNLDIRSFALNAEVSLLVYDEKVVARLREVE
                     AELFAQSRELALDAWQRRPGWVRTLQGIARLADSLL"
     gene            complement(617..1021)
                     /locus_tag="VFA35_11830"
     CDS             complement(617..1021)
                     /locus_tag="VFA35_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56908.1"
                     /translation="MRFLLPAILLAAAAAVPAQSCSLAVSGGAPGSTLSISVRGAPMS
                     LAAVAVGQTAGSTVIDLGSLGSLTLGLATPFVPLPLGIIDASGSASVSIPVPSQLPAL
                     DLVMQGVTVGITMMPLTLSLCATNVASVHVGA"
     gene            complement(1123..1782)
                     /locus_tag="VFA35_11835"
     CDS             complement(1123..1782)
                     /locus_tag="VFA35_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZT56909.1"
                     /translation="MDADRRRRMHPGRLRAAPTTMNATCTMIPGEAAAAAPAVEGFEE
                     LYAEFQPRVLAYCRAVLRHDEDALDASQDTMLAVFRHFDQLRQVLSVWNYLRRIAANK
                     CNDLLRRRQLHAHWLGSLRADGVEPIVVEHDSCDLLPTDEAQLQAGLMRSIARLSVRL
                     RRAVELRYVRGFDCRRIASELGISPGTVKSRLSRAREKLARSAGVAALRAHGLLGRQA
                     S"
     gene            complement(1848..2057)
                     /locus_tag="VFA35_11840"
     CDS             complement(1848..2057)
                     /locus_tag="VFA35_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56910.1"
                     /translation="MPSSDHHRLERLLARLRELERLRVPAHERPILEVARASLLEAIS
                     ALAHRLHDCDYFHRRPAAGSTGESG"
BASE COUNT          337 a          833 c          871 g          319 t
ORIGIN      
        1 cccgacatcg acgcgctgct gccgcaggtc gacttcgcgg gttcctcgcc ggcgcagctc
       61 ctgccgagcg ggcccggctt ccagcacgcg acgacgcacg acctcatcgt cgcgctgctg
      121 tacggcgccc aggagcgcgt cgtgctggcg acgccctact tcgtccccga cgacgccttc
      181 ctgcaggcgc tggcggcggc ggtgctgcgc ggcgcggagg tgcacctcgt cgtccccgag
      241 cgcggcaaca accggttcgt cgacctcgca ggcccgtcgt ggttcgagta cctgctggag
      301 gcgggcgtgc acgtccacct gcaccagccg cgcttcctgc acagcaagca cgtgagcttc
      361 gaccgcacga tcgcgctggt cgggtcgagc aacctcgaca tccgctcgtt cgcgctcaac
      421 gccgaggtca gcctgctggt ctacgacgag aaggtggtcg cgcggctgcg cgaggtcgag
      481 gcggaactgt tcgcgcagtc gcgcgagctc gcgctcgacg catggcagcg ccgccccgga
      541 tgggtgcgga cgctgcaggg gatcgcgcgg ctcgccgatt cgctgctctg acgagccgcg
      601 ccgatccgct acggaatcag gcgccgacgt ggaccgaggc gacgttcgtg gcgcagaggc
      661 tcagcgtcag cggcatcatc gtgatgccga ccgtcacgcc ctgcatgacc aggtcgagcg
      721 ccgggagctg gctcggcacc gggatcgaca ccgaggcgct gccgctggcg tcgatgatgc
      781 cgagcggcag gggcacgaac ggcgtcgcga ggccgagggt cagcgagccg aggctgccga
      841 ggtcgatcac ggtgctgccc gcggtctggc cgaccgcgac ggcggccagg ctcatcggcg
      901 cgcccctcac cgagatgctg agcgtgctgc ccggcgcgcc gccgctgacg gcgagcgagc
      961 aggactgggc ggggaccgcg gcggccgcgg cgaggaggat ggcagggagg aggaaacgca
     1021 tggtcattgt ggcttggaac tgggattgaa ggcgcaccga tggcgccgtg gaacgcacat
     1081 ccgccattgc ggcctccggc ccatcaccgc agccgagaaa aatcacgacg cctgccggcc
     1141 gagcaacccg tgtgcgcgca gcgccgcgac ccccgcgctg cgcgcaagct tctccctggc
     1201 acggctcaac cgcgacttca cggtgcccgg cgagatgccg agctcggacg cgatccggcg
     1261 gcaatcgaaa ccgcggacat agcgcaattc gacggcgcgc cgcagacgga ccgacagtct
     1321 ggcgatgctc cgcatcaggc cggcctgcag ctgcgcctcg tccgtcggca gtaggtcgca
     1381 ggaatcatgc tcgacgacga tcggctccac gccgtccgcg cgcagcgagc cgagccagtg
     1441 cgcgtgcagc tgccgccggc gcagcaggtc gttgcacttg ttggcggcga tgcggcgcag
     1501 gtagttccac accgacagca cctgtcgcaa ctggtcgaag tgccggaaca ccgcgagcat
     1561 cgtgtcctgg gaggcgtcga gggcgtcttc gtcgtggcgc aggacggcgc ggcagtacgc
     1621 gaggacgcgc ggctggaact ccgcgtagag ctcttcgaag ccctcgaccg cgggtgctgc
     1681 ggccgcggcc tcgccaggga tcatcgtgca ggttgcgttc atggtcgtcg gggcggcccg
     1741 aaggcgcccg gggtgcatcc gtcgccggcg gtccgcgtcc atcacgccgc gggcgttttc
     1801 cgccccgcca cgcgaacgtt gcacccgcgc cgcgcgcggc gcggccgtca gcccgattcg
     1861 ccggtgctgc cggcggcggg ccgccggtgg aagtagtcgc agtcgtgcag acggtgcgcg
     1921 agcgcgctga tcgcctcgag cagcgaggcg cgcgccactt cgaggatcgg ccgctcgtgc
     1981 gcaggcacgc gcagacgctc gagctcgcgc agccgggcga gcaggcgttc gagtcggtgg
     2041 tggtcgctcg agggcatcga tggggtcctg cggttcgggc ggcgcatcca tccgtcggac
     2101 gcgcgcagga accatcacgc ggcgcggtgc cgcgctgcct gcggccgcga gcaaacgtgg
     2161 ccggacgcgc gcttcctgcg catgccgcgc ggggggccgc ggtatcttcc gcgcatggtc
     2221 caacgcgaca cgacggaggc ccagatcacg gcgttgctgg gcaaggggaa gcggatgccg
     2281 gccgaccgcc tgctgccgct ggtctacgac gaactgcgca ggctcgcgca cgcgcgcgtc
     2341 gcccgcctcg gacccggaca
//
LOCUS       DASRDG010000706         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_227522, whole genome shotgun sequence.
ACCESSION   DASRDG010000706 DASRDG010000000
VERSION     DASRDG010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227522"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFA35_11845"
     CDS             complement(<1..917)
                     /locus_tag="VFA35_11845"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZT56911.1"
                     /translation="MTFSGDLSGISLPDVFQNLAGNRSTGTLHVRWDKGERFVRITDG
                     QIAGYSLGQGKELPLLDHVVERGYVDAAALQRAIAGRRRRKRPARLLLDEKLCDDETL
                     GTALSELTCEGVYDLLPLTAAQFSFAPGDPPADVFDPDLLAAGVRHDVGPLLLEGARR
                     ADETQRVRSVVGSDQDLFIADAQAHERAPDDTTAAVAAVLDGRTDVAGIARAVQMSRF
                     AVSSALLTLVQQGLARPATGEEIAAFAELALAEDSGEEAIRLLGQALVRMPREALLRR
                     RLAETLAAAGRKREAAGELAVLAFQAVEE"
     gene            complement(1006..2082)
                     /locus_tag="VFA35_11850"
     CDS             complement(1006..2082)
                     /locus_tag="VFA35_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56912.1"
                     /translation="MHDPHDGGRTARRRRTGQLFLLGLLTWVACSSLVPRHGWDKSLG
                     PVVPHDTFPDDCSLCHTGSDWHTLHADFAFDHRARTGVPLLGAHATATCLSCHNDRGP
                     AGRFAAQGCGGCHQDPHRGELGRGCKDCHEERTWQPSAMIQRHDRTRFPLVGAHAATA
                     CFRCHAGAQVGNFAGASTRCEDCHAGEYTRSTFDHVAAGFTSDCQHCHRPVGWKPAQF
                     SHPASFPLTLGHANRACSACHLPNTYSGLSRVCSSCHLDDYQRTTNPPHAAYAMPTTC
                     EQCHNTRTWGSGTFVHSWFPITSGAHRGFACVQCHTTQVGPVFSCTSCHTHAQSPMAS
                     THSGVRGYSWVSSECYRCHPNGRR"
     gene            complement(2098..3786)
                     /locus_tag="VFA35_11855"
     CDS             complement(2098..3786)
                     /locus_tag="VFA35_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56913.1"
                     /translation="MKPRRLHRAPLLCLLAVASVATPAVAQSEPSGQQRTVRVQVTGV
                     SGRDVYLDQGRAQGLEAGMVARLFPPGLGQLEVEIRSVSSSSARAELPPGAPPPPVGT
                     RGEVAVPDAPAPTSTPPAAGGDRRPVPDHPPWTRQEQARGADQPLLVPTYGQRPDERP
                     FTLDGRLFASTQYQLDTGGARNSSTLLSRLGIWSEGHNALGTGERLRLAGEFDDRRAQ
                     FSDQPDTDEASGRVDLASVAVGTEDWAPLGLEAGRFVSDGLPELGLIDGVEGVLRCEH
                     GLRFGAGVGSYPLPFPGRDTGDDLGFHAFADYVADDRRSFAAAVGVQKTWHQGAPDRD
                     LLLLRADGRPAEGFWLQGSAKIDLYTAGDTLKGAGPELTEFLGQARFDGARCGTGLAL
                     SEFRWPDLLRAEFLNLPPALVRSGHVERASWSGWLRASDTLRLSARADLWRDQDDHGT
                     AFELAGDCADVWGRGSVLSAAVFTTDGGTLSGPGLRVDFRRAFGVVQAHAGYRWYRYD
                     VTSLLAGNETYSRQSVELGLDWAVGDFDLDFTVERWFGDQEDAFALGIFAQWRF"
     gene            complement(3783..>4498)
                     /locus_tag="VFA35_11860"
     CDS             complement(3783..>4498)
                     /locus_tag="VFA35_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56914.1"
                     /translation="PRYAQCTACHRDAAFAPHTFDVAAHAHTAFPLTGSHQAVGCALC
                     HRRELQHGVRAFHGEHRECGACHQDVHQGRFDGAGRPAQVGGRVGCSRCHDTAAFAPA
                     AAFDHALWTGYPLDGAHAGAACIGCHQPQKGRGERRLGPAAGRRCADCHRDVHQGQLA
                     QAGATDCARCHDQTLWKPSHFDHQRDSRFLLDKQHRDLACASCHKPYPSASGPVIRYK
                     PLGTSCGDCHQLGSKGGRR"
BASE COUNT          553 a         1604 c         1747 g          594 t
ORIGIN      
        1 tcctcctcga cggcctggaa ggccagcacc gcgagctcgc ccgcggcctc gcgtttgcgg
       61 cccgcggcgg cgagcgtctc ggcgagccgg cggcgcagca gcgcctcgcg cggcatgcgg
      121 accagcgcct ggccgagcag ccggatcgcc tcctcgcccg agtcctcggc gagcgccagt
      181 tcggcgaacg ccgcgatctc ctcgccggtc gccggccgcg cgagcccctg ctgcacgagc
      241 gtgagcagcg cggagctgac ggcgaagcgg ctcatctgca cggcgcgcgc gatgccggcg
      301 acgtcggtac ggccgtcgag caccgcggcg accgcggcgg tggtgtcgtc gggcgcgcgt
      361 tcgtgcgcct gcgcgtcggc gatgaacagg tcctggtcgg agccgaccac cgagcgcacg
      421 cgctgcgtct cgtcggcgcg gcgcgcacct tcgagcagca gcggcccgac gtcgtgccgc
      481 accccggcgg cgagcaggtc gggatcgaag acgtcggctg gcggatcgcc gggcgcgaac
      541 gagaactgcg ccgcggtgag cggcagcagg tcgtagacgc cctcgcaggt cagctcggac
      601 agcgcggtgc cgagcgtctc gtcgtcgcac agcttctcgt cgagcagcag gcgcgcgggg
      661 cgcttgcgcc ggcgccggcc cgcgatcgcc cgctgcagcg cggcggcgtc gacgtagccg
      721 cgctcgacga cgtggtcgag cagcggcagc tccttgccct ggcccagcga atagccggcg
      781 atctggccgt cggtgatgcg cacgaagcgt tcgcccttgt cccagcgcac gtgcagcgtg
      841 ccggtggacc tgttgccggc caggttctgg aacacgtccg gcaggctgat gcccgacagg
      901 tcaccggaga acgtcatgct tgacctcctt tcggccgcgc ggccgcggcg gcgagagcgc
      961 gcgccgaacc gccgttggca ccgacggcat gcggcccgcg ccggctcatc tgcggccgtt
     1021 cgggtggcag cggtagcact cgctcgacac ccagctgtag ccgcgcacgc cgctgtgcgt
     1081 gctcgccatc ggcgactgcg cgtgcgtgtg gcagctcgtg cacgagaaca ccgggccgac
     1141 ctgggtcgtg tggcactgga cgcaggcgaa cccgcggtgc gcgccgctgg tgatcgggaa
     1201 ccagctgtgc acgaacgtgc ccgagcccca ggtgcgcgtg ttgtggcact gttcgcaggt
     1261 ggtcggcatc gcgtacgcgg cgtgcggcgg gttggtcgtg cgctggtagt cgtcgaggtg
     1321 gcagctcgag cagacgcggc tgaggccgga gtaggtgttc ggcaggtggc atgccgagca
     1381 ggcgcggttg gcgtggccga gcgtgagcgg gaagctcgcg gggtgcgaga actgcgccgg
     1441 cttccagccg acgggccggt ggcagtgctg gcagtcgctg gtgaagccgg ccgcgacgtg
     1501 gtcgaacgtg gagcgcgtgt actcaccggc gtggcagtcc tcgcagcgcg tgctcgcgcc
     1561 cgcgaagttg ccgacctgcg cgcccgcgtg gcagcggaag cacgcggtcg ccgcgtgcgc
     1621 gccgaccagc gggaagcggg tgcggtcgtg ccgttggatc atcgcgctcg gctgccaggt
     1681 gcgctcttcg tggcagtcct tgcagccgcg gccgagttcg ccgcggtgcg ggtcctggtg
     1741 gcagccgccg cagccctgcg ccgcgaagcg gcctgccggc ccgcggtcgt tgtggcaaga
     1801 gaggcaggtc gcggtcgcgt gcgcgccgag cagcggcacg ccggtgcgtg ctctgtggtc
     1861 gaatgcgaag tcggcgtgca gcgtgtgcca gtccgagccg gtgtggcaga gcgagcagtc
     1921 gtccgggaag gtgtcgtgcg gcacgacggg accgaggctc ttgtcccagc cgtggcgcgg
     1981 caccagcgag ctgcacgcga cccaggtcag gaggccgagc aggaacagct ggccggtccg
     2041 gcgccgccgc gccgtcctgc cgccgtcgtg tgggtcatgc atgttgcgtt ctccgtttca
     2101 gaagcgccac tgcgcgaaga tgccgagcgc gaacgcatcc tcctgatcgc cgaaccagcg
     2161 ttcgacggtg aagtcgaggt cgaagtcgcc gaccgcccag tcgaggccga gctcgaccga
     2221 ctgccgcgag taggtctcgt tgcccgcgag cagcgaggtc acgtcgtagc ggtaccagcg
     2281 gtagcccgcg tgcgcctgga ccacgccgaa cgcccgccgg aagtcgacgc gcagccccgg
     2341 gcccgacagg gtgccgccgt cggtcgtgaa caccgccgcc gacagcaccg acccacgccc
     2401 ccagacgtct gcgcagtcgc ccgcgagttc gaacgcggtg ccgtggtcgt cctggtcgcg
     2461 ccacaggtcg gcgcgtgccg acaggcgcag cgtgtcgctc gcgcgcagcc agccgctcca
     2521 cgacgcgcgt tcgacgtggc ccgagcgcac gagcgccggc ggcaggttca ggaactccgc
     2581 gcgcagcagg tcgggccagc ggaactcgga cagcgcgagc ccggtgccgc agcgtgcgcc
     2641 gtcgaagcgc gcctggccga ggaactcggt cagctcgggg ccggcgccct tcagcgtgtc
     2701 gccggcggtg tagaggtcga tcttggcgct gccctgcagc cagaagcctt cggcaggccg
     2761 gccgtcggcg cgcagcagca gcaggtcgcg gtcgggcgcg ccctggtgcc aggtcttctg
     2821 cacgccgacc gcggcggcga agctgcgccg gtcgtcggcg acgtagtcgg cgaacgcatg
     2881 gaagccgagg tcgtcgccgg tgtcgcggcc cgggaacggc agcgggtagc tgccgacgcc
     2941 tgcgccgaag cgcagcccgt gctcgcagcg cagcacgccc tcgacgccgt cgatcagccc
     3001 cagctcgggc agcccgtcgg agacgaagcg gccggcctcg agcccgaggg gcgcccagtc
     3061 ctcggtgccg accgcgaccg acgcgaggtc gacgcggccg ctggcttcgt cggtgtccgg
     3121 ctggtcgctg aactgcgcgc ggcggtcgtc gaactcgccc gcgagccgca gccgttcgcc
     3181 ggtgccgagc gcgttgtggc cctcgctcca gatgccgagc cgcgacagca gcgtcgagct
     3241 gttgcgcgcg ccgccggtgt cgagctggta ctgcgtcgac gcgaacagcc ggccgtcgag
     3301 cgtgaacggc cgttcgtcgg ggcgctggcc gtaggtcgga accagcagcg gctggtcggc
     3361 gccgcgcgcc tgctcctggc gcgtccacgg cggatggtcg ggcaccggcc gccggtcgcc
     3421 accggccgcc ggcggcgtgg acgtgggcgc gggcgcatcg ggcacagcga cctcgccgcg
     3481 cgtgccgacc ggcggcggcg gcgcgccggg cggcagttcc gcgcgcgcgg acgaactgct
     3541 gacgctgcgg atctcgacct cgagctggcc gagccccggc gggaacaggc gcgcgaccat
     3601 gcccgcctcc aggccctgcg cgcggccctg gtccaggtag acgtcgcggc ccgagacgcc
     3661 ggtgacctgg acgcgcacgg tgcgctgctg gcccgacggc tccgactgcg cgaccgccgg
     3721 cgtcgcgacc gatgccacgg cgagcaggca cagcagcggc gcccggtgca gccgtcttgg
     3781 cttcatcgcc ggcctccctt gctgccgagc tgatggcagt cgccgcacga tgtgccgagc
     3841 ggcttgtagc ggatcaccgg cccggacgcc gacgggtacg gcttgtggca gctcgcgcag
     3901 gcgaggtcgc ggtgctgttt gtcgagcagg aagcggctgt cgcgctggtg gtcgaagtgc
     3961 gacggcttcc agagcgtctg gtcgtgacag cgcgcgcagt cggtcgcgcc ggcctgcgcg
     4021 agctggccct ggtgcacgtc gcggtggcag tcggcgcagc gcctgcccgc ggccggcccg
     4081 aggcgccgtt cgccgcggcc cttctgcggc tgatggcagc cgatgcacgc ggccccggcg
     4141 tgcgcgccgt cgagcgggta gccggtccac agcgcgtggt cgaacgcggc cgccggcgcg
     4201 aacgccgcgg tgtcgtggca gcgcgagcag ccgacgcggc cgccgacctg cgccggcctg
     4261 ccggcgccgt cgaaccgccc ctggtgcacg tcctggtggc acgcgccgca ctcgcggtgc
     4321 tcgccgtgga aggcgcgcac gccgtgctgc agttcgcgcc ggtggcacag cgcgcagccg
     4381 accgcctgat ggctgccggt cagcgggaac gccgtgtgag catgtgccgc gacgtcgaac
     4441 gtgtgcggcg cgaacgccgc gtcgcggtgg caggccgtgc actgcgcgta gcgcgggt
//
LOCUS       DASRDG010000707         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1146029, whole genome shotgun sequence.
ACCESSION   DASRDG010000707 DASRDG010000000
VERSION     DASRDG010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146029"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..806
                     /locus_tag="VFA35_11865"
                     /pseudo
     CDS             1..806
                     /locus_tag="VFA35_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922074.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
     gene            complement(822..1439)
                     /locus_tag="VFA35_11870"
     CDS             complement(822..1439)
                     /locus_tag="VFA35_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HZT56915.1"
                     /translation="MRPPHGACVVLVDAGTPAADGTCDATPRAVRQALESGAPAVIVL
                     CAGDAPLTRARGTLRALGQRLAPLAVEFGARVSRSATDGLAAWAASRAAPIGIDVQAA
                     PAALDADLLGAALHADEQAWLAAQPDSTLAFAQLWAGKEAVLKAFGVGLAWSPREVVA
                     LPVANGWHGSNVPALGHAWLSRVVAPGGRRVAVAVALNAGFARPH"
     gene            complement(1444..>2175)
                     /locus_tag="VFA35_11875"
     CDS             complement(1444..>2175)
                     /locus_tag="VFA35_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZT56916.1"
                     /translation="FTRVMLLVPGAGGHAAALYALADELRAGLPPGSAVAIVDLDAAL
                     HASPHDDPMWFISRRIVQVVRDLGQPRVLGIVGYSLGGAIALRVAEALQADPPLPVWL
                     LDTFAPRTNRPSFWRKLERRLAWLLFGGRPRHDAADASTSAAAHALPMSATPEQWSVL
                     GRQLSNATLAAPRAAVRLIQARMSVKYHGLLWQRRNNGFLPRHYASWKVHEIDGAHLD
                     LPRHLAATTARIIVGHGQSGPRGAG"
BASE COUNT          293 a          794 c          805 g          283 t
ORIGIN      
        1 gaaccggctc gcccaggtgc tcggtgcgcg ggcgtttgcc aatgactcga ttgccttcac
       61 cgaccaactg ccgctgacct ttcgcggcgc ggtgcaggtg ccgaacctcg tgctcgccag
      121 cctggcccgg ccatcgttcg acgcgcgcca ggcggcgctg atcaatggca tgtaccgggg
      181 ctcgccgctc gccggcatgg tgagcgaggg tttcgcggtg cgcattgaag cgatgcgcga
      241 catccgcggc gatgcgagga acgagggcat ggtgaacgag atgctcgccg ccagccgcaa
      301 cgcgatcacc gcgaaaggct tcgagggcga ggcgcggcgc atcgcgcggc tgatgcgcga
      361 gcgctaccac ctcggcttcg tcgacgtggg cggctgggac acccacgtcg gccagggcgc
      421 cgccagcggc tacctcgcga accggctcgc cgaactcggc aagggcctgg ccgcttacgc
      481 cgacgaaatg ggcccggcgt ggcgcgacgc caccgtcgtc gtgctgcgaa ttcggtcgca
      541 ccttcaagga gaacggcaac cgcggcaccg accacggcca cggcagcgtc tactgggtgc
      601 tcggcggcgg cgtgcgcggc ggccgcgtcg tcggcgagca ggtcgcggtg gcgccgggaa
      661 gcctgttcca gaaccgcgac tacccggtgc tgaacgagta ccgcagcttg ttcggcgggt
      721 tgttcgccgg cctgtacggg ttgtcgcccg cgcagctgca gcaggtgttt ccgggcgcgc
      781 ccgcgcgcgg catcggcttg gtctgagcct gcgctccgcc ctcagtgcgg ccgcgcgaag
      841 ccggcgttca gggcgaccgc cacggcgacc ctccgcccgc ccggcgcgac gacccggctg
      901 agccacgcgt gaccgagcgc cggcacgttc gacccgtgcc agccgttcgc taccggcagc
      961 gccacgactt cgcgcggcga ccaggccagc ccgacgccga aggccttcag caccgcttcc
     1021 ttgccggccc agagttgcgc gaacgcgagg gtcgagtccg gctgagccgc gagccaggct
     1081 tgttcatccg catgcagcgc ggcgccgagc agatcggcgt cgagtgccgc gggcgccgcc
     1141 tgcacgtcga tcccgatcgg cgccgcgcgg ctcgcggccc atgccgccaa cccgtcggtg
     1201 gcgctgcggc tgacccgcgc gccgaactcg accgcgagcg gcgcgaggcg ttgaccgagt
     1261 gcgcgcagcg tcccgcgcgc gcgggtcaac ggcgcatcgc cggcacagag cacgatgacg
     1321 gcgggcgcgc cggattcgag cgcttgccgc accgcgcgcg gcgtcgcgtc gcacgtgccg
     1381 tcggcggccg gggtgccggc atccaccagc acgacgcatg cgccgtgcgg tgggcgcatt
     1441 gcgtcagccg gcaccgcgcg gaccggactg cccgtggccg acgatgatgc gcgccgtcgt
     1501 cgcggccaga tggcgcggca ggtcgaggtg cgcaccatcg atctcgtgga ccttccacga
     1561 cgcgtagtgg cgcggtagga agccgttgtt gcggcgctgc cagagcagcc cgtggtactt
     1621 gacgctcatg cgcgcctgga tcagccgcac cgcggcccgc ggcgccgcca gggtggcgtt
     1681 gctgagctgc cggcccagca cgctccactg ttccggcgtg gcgctcatcg gcagcgcgtg
     1741 cgctgcggca ctcgtcgacg cgtccgccgc gtcgtggcgc gggcgcccgc cgaacagcag
     1801 ccacgcgagt ctgcgttcca gcttgcgcca gaagctcggc cggttggtgc gcggcgcgaa
     1861 cgtgtcgagc agccagaccg gcagcggcgg atcggcctgc agcgcctcgg ccacccgcag
     1921 cgcgatcgcg ccccccagcg agtagcctac gatgccgagc acgcgcggct ggccgaggtc
     1981 gcgcacgacc tggacgatgc ggcgcgagat gaaccacatc gggtcgtcgt gcggcgacgc
     2041 gtgcagcgcg gcgtcgaggt cgacgatggc caccgcgctg cccggcggca ggccggcgcg
     2101 cagttcgtcg gcgagcgcgt agagcgcggc cgcatggcca ccggcgcctg gcaccagcag
     2161 catcacacgg gtgaa
//
LOCUS       DASRDG010000708         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_227714, whole genome shotgun sequence.
ACCESSION   DASRDG010000708 DASRDG010000000
VERSION     DASRDG010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227714"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /gene="coaBC"
                     /locus_tag="VFA35_11880"
     CDS             <1..721
                     /gene="coaBC"
                     /locus_tag="VFA35_11880"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZT56917.1"
                     /translation="IGDGRMLEPEEILAALLSWAQPKQLAGKRVLLTAGPTFEAIDPV
                     RGITNHSSGKMGFAIARAAAEAGASVTLVAGPVHLPTPRQVRRIDVVTAQQMFEAVSA
                     MAGAHDVFVATAAVADWRPARCDERKIKKQAGQKTPPSLELTENPDILAAVARLPQPP
                     YCVGFAAESHELLKHAREKRVRKGVPLIVANLGPATFGQDDNALTLVDAAGERELPRA
                     DKLTLARQLVAEIAARLGAAA"
     gene            718..1167
                     /gene="dut"
                     /locus_tag="VFA35_11885"
     CDS             718..1167
                     /gene="dut"
                     /locus_tag="VFA35_11885"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZT56918.1"
                     /translation="MSVIDVKVLDARMAEQLPAYATPGSAGLDLRACVDAALIIEPGQ
                     AVLIPTGLAIHIGDPGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRG
                     QEAFTVQPLDRVAQLVIVPVVQAAFRVVAEFGASDRGAGGFGSTGRA"
     gene            1338..2549
                     /locus_tag="VFA35_11890"
     CDS             1338..2549
                     /locus_tag="VFA35_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZT56919.1"
                     /translation="MGSAAMLAVALAVGTAGCSKEPAPLAAASAASGAAPAGAASGAG
                     GPVSVSSVRAVARDTDVMLEATGTISALNSVDIRPQVSSVIMQVNIREGQFVKAGQLL
                     FTLDARNDEVNLAKAKAQLARDQASLADAQRQLARSRELLGQGFISQGAVDTNQTLVD
                     AQQAVVSADRAAIQAALVGLSYNRIVAPAAGRAGAINVYVGSTVQPAGAALVTITQLD
                     PIAVAFSLPQRNLNDALQTLRSGGGKVVAVLPESRGELTGKLQFVDNVVDPNSGTVKV
                     KAQFDNANEALWPGAFVTVRLAVNTLKGAVVVPQAAVIQGPRGAVVYAVDADDKAVSR
                     RVEVLYANGPDAVVGGVRAGERIVVEGRQNLRPGVTVVERAATGAAGSRAAPAPGTGA
                     SSSGAGPAATP"
     gene            2557..>3694
                     /locus_tag="VFA35_11895"
     CDS             2557..>3694
                     /locus_tag="VFA35_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZT56920.1"
                     /translation="MNLSELFIRRPVLTVLLNAAIVVAGVIAYGRIPVAALPSYNTPV
                     INVFAQLPGASPEAMATSVALQLEKQFSTIPGLAIISSSNTLGSTNLTLEFDQGRDID
                     AASVDVQAALLRAQRSLPQDMTAPPSYRKVNPADAPVLLLALTSPSLNLSDLNDYAEH
                     LISPTLSTLEGVAQVNIFGQKRYAVRVRVLPQALSARNIGLDELSAALTAANVNTPVG
                     TLEGPQQTLTLQANRQLRNAAEFADLIVSTRGGNPVRLRDVAAVEDSFETVKTFASFN
                     GERSITLAVLRQPDANTVKVVDAVKAAMPNFQSQLPQSVRVSLVNDRSVSVREALHDV
                     SLTMLGTIVLVVLVIFLFLRRFMATVIPTLSLPVSLLGGVALLWG"
BASE COUNT          563 a         1319 c         1290 g          522 t
ORIGIN      
        1 aatcggtgac ggcaggatgc tcgagccgga ggagatactg gccgcattgc tatcgtgggc
       61 ccagcctaag caactcgccg gaaagcgcgt gctcctcacc gccgggccga ccttcgaggc
      121 catcgacccg gtgcgcggca tcaccaacca ctcgagcggc aagatgggct tcgcgatcgc
      181 acgcgccgcg gccgaggcgg gcgcgagcgt cacgctcgtc gccggcccgg tgcatctgcc
      241 gacgccgcgc caggtgcgcc gcatcgacgt ggtgacggcg cagcagatgt tcgaagcggt
      301 cagcgccatg gcgggcgcgc acgacgtgtt cgtcgcgacc gccgcggtcg ccgactggcg
      361 gccggcgcgc tgcgacgagc gcaagatcaa gaagcaggcg ggccagaaga cgccgccgag
      421 cctcgaactg accgagaacc ccgacatcct cgccgcggtg gcgcggctgc cgcagccgcc
      481 gtactgcgtc ggcttcgcgg ccgagagcca cgagctgctg aagcatgccc gcgagaagcg
      541 ggtgcgcaag ggcgtgccgc tgatcgtcgc caacctcggc ccggcgacct tcggtcagga
      601 cgacaacgcg ctgacgctgg tcgatgcagc cggcgagcgt gagttgccgc gcgccgacaa
      661 gctgacgctg gcgcggcaac tggtcgccga gattgcggcg cgcctcggag ctgcagcgtg
      721 agcgtgattg acgtcaaggt gctcgatgcg cgcatggccg agcaactccc cgcctatgcg
      781 acccccggca gcgccgggct cgacctgcgc gcctgcgtcg atgcggcgct gatcatcgaa
      841 cccggccaag ccgtgctgat cccgacgggt ttggccatcc acatcggcga cccgggcctg
      901 gccgcaatga tcctgccgcg ctcgggcctc ggccacaagc acggcatcgt gctcggcaac
      961 ctggtcgggc tgatcgattc cgactaccag ggcccgctga tggtgagctg ctggaaccgc
     1021 gggcaggagg cgttcacggt gcagccgctc gaccgcgttg cacagctcgt gatcgtgccg
     1081 gtggtgcagg cggcctttcg ggtcgtggcc gagttcggcg cgtcggatcg cggcgccggc
     1141 ggcttcggct cgaccggacg cgcctgagcc caacggaact cgcgcggcca gtgcgcgcac
     1201 aaacccgcag ttcggccgca gtttcgggca cgcgcgttga acccgatccg ccaattggcg
     1261 agaatcacaa gctgtaacga attggcgcgg ttcgcggcga acggcgccca cctgggatct
     1321 gcatgaacaa gcgttggatg ggttcggccg caatgctcgc ggtcgcgctc gcggttggca
     1381 ccgcgggctg cagcaaggag cccgcgccgt tggcggccgc gtcggccgcc tcgggcgccg
     1441 cgccagccgg ggcagcgtcg ggcgcgggcg gcccggtcag cgtcagcagc gtgcgcgcgg
     1501 tcgcgagaga cacagacgtg atgctcgaag ccaccggcac gatcagcgcg ttgaacagcg
     1561 tcgacatccg gccgcaggtc agcagcgtga tcatgcaggt caacatccgc gaaggccagt
     1621 tcgtgaaggc ggggcagttg ctgttcacgc tcgatgcgcg caacgacgag gtcaacctcg
     1681 ccaaggccaa ggcccagctc gcgcgcgacc aggcctcgct cgccgatgcg cagcgccagc
     1741 tcgcgcgcag ccgcgaactg ctcgggcagg gcttcatctc gcagggcgcg gtcgacacca
     1801 accagaccct ggtcgatgcg cagcaagccg tcgtcagcgc cgaccgcgcc gcgatccagg
     1861 ccgcgctcgt cggcttgtcg tacaaccgga tcgtcgcgcc cgcggccggg cgcgccggcg
     1921 cgatcaacgt ctacgtcggc agcacggtgc agccggccgg cgccgcgctc gtgacgatca
     1981 cccagctcga cccgatcgcc gtcgcgttca gcctgccgca gcgcaacctg aacgacgcgc
     2041 tgcagacgct gcgctcgggc ggtggcaagg tcgtcgcggt gctgcccgag tcgcgcggcg
     2101 agctcaccgg caagctgcag ttcgtcgaca acgtggtcga cccgaactcg ggcacggtca
     2161 aggtcaaggc ccagttcgac aacgcgaacg aggcgctgtg gcccggcgca ttcgtcacgg
     2221 tgcggctggc cgtcaacacg ctgaagggcg cggtcgtcgt gccgcaggcc gcggtgatcc
     2281 aggggccgcg cggcgccgtc gtctatgccg tcgacgccga cgacaaggcg gtctcgcggc
     2341 gcgtcgaggt gctgtacgcg aacggccccg atgcggtggt cggcggcgtg cgcgccggtg
     2401 agcgcatcgt cgtcgagggc cgccagaacc tgcggcccgg cgtcacagtg gtcgagcgtg
     2461 ccgcgaccgg cgccgccggc tcgcgcgcgg ccccggcgcc cggcaccggc gccagttcat
     2521 cgggcgccgg cccggccgcg accccatgaa cgcgccatga acctctccga gctcttcatc
     2581 cgccggccgg tgctgacggt gctgctgaac gcggcgatcg tcgtcgccgg ggtgatcgcc
     2641 tatggccgca tcccggtcgc ggcgctgccg agctacaaca cgccggtcat caacgtgttc
     2701 gcgcaactgc ccggtgcgag ccccgaggcg atggcgacct cggtcgcgtt gcagctcgag
     2761 aagcagttct cgaccatccc gggcctcgcc atcatcagct ccagcaacac gctgggcagc
     2821 accaacctga cgctcgagtt cgaccagggc cgcgatatcg acgccgcctc ggtcgacgtg
     2881 caggccgcgc tgctgcgcgc gcagcgatcg ctgccgcagg acatgaccgc gccgccgtcg
     2941 taccgcaagg tcaaccccgc cgatgcgccg gtgttgctgc tcgcgctcac ctcgccgtcg
     3001 ctgaacctgt cggacctcaa cgactacgcc gaacacctga tctcgccgac gctgtcgacg
     3061 ctcgaaggcg tcgcgcaggt caacatcttc ggccagaagc gctacgcggt gcgcgtgcgc
     3121 gtgctgccgc aggcactgtc ggcgcgcaac atcggcctcg acgaactgag cgcggcgctc
     3181 accgccgcga acgtcaatac cccggtcggc acactcgaag gcccgcagca gacgctgacg
     3241 ctgcaagcca accgccagct gcgcaatgcc gccgagttcg ctgacctgat cgtcagcacg
     3301 cgcggcggca acccggtgcg gctgcgcgac gtggccgcag tcgaagacag tttcgagacc
     3361 gtcaagacct ttgccagctt caacggcgag cgctcgatca cgctcgcggt gctgcgccag
     3421 cccgacgcga acaccgtgaa ggtagtcgac gcggtgaagg ccgcgatgcc gaacttccag
     3481 tcgcaactgc cgcagtcggt gcgcgtctcg ctggtcaacg accgctcggt ctcggtgcgc
     3541 gaggcgctgc acgacgtgtc gctgacgatg ctcggcacga tcgtgctggt ggtgctggtg
     3601 atcttcctgt tcctgcggcg cttcatggcg acggtgatcc cgacgctgtc gctgccggtc
     3661 tcgctgctcg gcggcgtcgc gctgctgtgg ggct
//
LOCUS       DASRDG010000709         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1718660, whole genome shotgun sequence.
ACCESSION   DASRDG010000709 DASRDG010000000
VERSION     DASRDG010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718660"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VFA35_11900"
     CDS             complement(<1..1018)
                     /locus_tag="VFA35_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZT56921.1"
                     /translation="MRLTSAALCGTALCCTAAQLPALAQTAAAPPGAQLAPVVVTATR
                     VDARAFDVPASIDRIDGEAIRDARAQVNISESLGGVPGVLARDRQNYAQDVQISVRGF
                     GARSTFGIRGVRLYVDGIPATLPDGQGQISNVDLGSVERIEVLRGPFSALYGNSSGGV
                     IQVFTEEGSGAPRLSFGAAGGSDGMLRLSARASGASGALGYTVSASHFRTDGYRDHSR
                     TERNIGNAKLTIHPDDASKLTLIANSVALPQAQDPLGLSRAQFEADPRGVDPSANAFD
                     TRKTVNQTQGGLVFERRVDAANALQVSAYLGHRGTQQYQSIPVATQNNPLHPGGVIVL
                     GRNYE"
     gene            complement(1074..1925)
                     /locus_tag="VFA35_11905"
     CDS             complement(1074..1925)
                     /locus_tag="VFA35_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZT56922.1"
                     /translation="MIRDPSGRRACLRRLALAAGAAGAACALPAAAITLAKVRERGAL
                     VVGVYNDMPPFHAAGKGIDVDLAGALAASLGLKLSLLPFNADENMNDDLRNLVWKGHY
                     LGFGPADVLMHVPVDRPLMDATPQATIFAPYYRERVVLARNLAQLPTLETLQPLAGQR
                     VAVPGQSLAGWLLLGADGGAYRNQLSTQWKDGTECARALQRGEVTVAAGLASELESVL
                     LGDARFAIEPLPSPRAPRNGWATGLAVKSDATDLAQALQTSMNQLAQQGRLKDIFARY
                     NVAWRAP"
     gene            complement(1922..2419)
                     /gene="pedF"
                     /locus_tag="VFA35_11910"
     CDS             complement(1922..2419)
                     /gene="pedF"
                     /locus_tag="VFA35_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HZT56923.1"
                     /translation="MQPNASPRRARLSCLVSAATAGVLVIAAAAALAHGDVTPQAVDT
                     SSLPKLGDAWRAENPYRGNEAAVKVGTSAYNQNCARCHGLEAISGGIAPDLRKLDNDC
                     ATVQDAARKAACVKDNDEYFLTTVRHGRTRNGAVYMPPFEGTFNQEAIWAIKAYLETR
                     RDKPL"
BASE COUNT          308 a          845 c          929 g          380 t
ORIGIN      
        1 cctcgtagtt gcggccgagc acgatcacgc cgccggggtg cagcgggttg ttctgcgtcg
       61 ccaccggaat cgactggtat tgctgcgtgc cgcggtggcc gaggtaggcc gagacctgca
      121 gcgcgttcgc ggcgtcgacg cggcgctcga agaccaggcc gccctgcgtc tggttgacgg
      181 tcttgcgcgt gtcgaacgcg ttcgcagagg ggtcgacgcc gcgcgggtcg gcctcgaact
      241 gcgcgcgcga caggccgagc gggtcttgcg cctgcggcag cgccacgctg ttggcgatca
      301 gcgtcaactt gctcgcgtcg tccggatgga tcgtcagctt ggcgttgccg atgttgcgct
      361 cggtgcggct gtggtcgcgg tagccgtcgg tgcggaagtg gctcgcgctg accgtgtagc
      421 cgagcgcgcc gctcgcgccg ctggcccggg ccgacaggcg cagcatgccg tcgctgccgc
      481 cggccgcgcc gaagctcagg cgcggcgcgc cgctgccttc ctcggtgaag acctggatca
      541 cgccgcccga cgagttgccg tacagcgccg agaacggccc gcgcagcacc tcgatgcgct
      601 cgaccgagcc gaggtcgacg ttcgagatct ggccctgccc gtcgggcagc gtcgccggga
      661 tgccgtcgac gtacaggcgc acgccgcgga tgccgaaggt cgagcgcgcg ccgaagccgc
      721 gcaccgagat ctgcacgtcc tgcgcgtagt tctgccgatc gcgcgcgagc acgcccggca
      781 caccgcccag gctctcggag atgttgactt gtgcgcgcgc gtcgcggatc gcttcgccgt
      841 cgatgcggtc gatcgacgcc ggcacatcga acgcgcgcgc gtcgacgcgc gtcgcggtca
      901 cgaccaccgg cgcgagttgc gcgcccggcg gcgcggcggc ggtctgtgcc agcgcgggca
      961 gctgggcggc cgtgcagcac aaggccgtgc cgcacaacgc ggcggatgtc agtcgcatgt
     1021 cgtctccgat gttcttctcg gtcggccccg cctgcgcggg tctgctcgtg ttgtcagggc
     1081 gcgcgccagg ccacgttgta gcgcgcgaag atgtctttga gccgcccttg ctgcgccagc
     1141 tggttcatgc tggtctgcag cgcctgggcc aggtcggtgg cgtcgctctt gaccgccagg
     1201 ccggtggccc agccgttgcg cggtgcgcgc ggcgagggca gcggctcgat cgcgaagcgg
     1261 gcgtcgccga gcagcaccga ttcgagctcg gaggccagcc cggccgcgac cgtgacctcg
     1321 ccgcgctgca aggcgcgcgc gcactcggtg ccgtccttcc actgcgtcga cagctggttg
     1381 cggtacgcgc cgccgtcggc gccgagcagc agccagccgg ccagcgactg gcccggcacc
     1441 gcgacgcgct ggcccgcgag cggctgcagc gtttcgagcg tcggcagctg cgccaggttg
     1501 cgcgcgagca cgacgcgctc gcggtagtac ggcgcgaaga tcgtcgcctg cggcgtcgcg
     1561 tccatcagcg gccggtcgac cggcacgtgc atcagcacat cggccgggcc gaagccgagg
     1621 tagtggccct tccagaccag gttgcgcagg tcgtcgttca tgttctcgtc ggcattgaac
     1681 ggcagcagcg agagcttcag accgagcgac gcggcgagcg cgccggcgag gtcgacgtcg
     1741 atgcctttgc ccgcggcgtg gaacggcggc atgtcgttgt acacgccgac gaccagcgcg
     1801 ccgcgctcgc gcaccttggc cagcgtgatc gcggcggcag gcagcgcaca ggccgcgccg
     1861 gcagcaccgg cggcaagggc caggcggcgc aggcaggcgc gccggccgga tggatcgcgg
     1921 atcacagcgg cttgtcgcgg cgggtctcga ggtaggcctt gatggcccag atcgcctcct
     1981 ggttgaaggt gccctcgaac ggcggcatgt agaccgcgcc gttgcgcgtg cggccgtggc
     2041 gcacggtggt gaggaagtac tcgtcgttgt ccttcacgca cgcggccttg cgcgccgcgt
     2101 cctgaacggt tgcgcagtcg ttgtcgagct tgcgcaggtc gggcgcgatg ccgcccgaga
     2161 tcgcttccag gccgtggcag cgcgcgcagt tctggttgta agccgaggtg ccgaccttca
     2221 ccgcggcctc gttgccgcga taggggttct cggcgcgcca ggcatcgccg agcttgggca
     2281 ggctcgaggt gtcgaccgcc tggggcgtga cgtcgccgtg ggcgagcgcc gcggccgcgg
     2341 cgatcaccag cacgccggcg gtcgcggccg aaaccaggca ggacagccgc gcacggcggg
     2401 gggaagcgtt cggttgcatg ggttgtctcc gttctaggta ggattccgac tcgctggcgt
     2461 tt
//
LOCUS       DASRDG010000710         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_685248, whole genome shotgun sequence.
ACCESSION   DASRDG010000710 DASRDG010000000
VERSION     DASRDG010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685248"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFA35_11915"
     CDS             complement(<1..466)
                     /locus_tag="VFA35_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZT56924.1"
                     /translation="MNPVAATPARRIAHLDMDAFYASVELLRYPQLAAQPVVIGGRRR
                     VSGEGAEGFALLADYQGRGVVTTATYPAREFGVRSGMALMHAARLCPRAVLLPADFDE
                     YRRYSRLFKAAIAAIAPRIEDRGIDEVYIDFDAVTGGAEEGGLPLARRIQQAV"
     gene            complement(463..1932)
                     /gene="hutI"
                     /locus_tag="VFA35_11920"
     CDS             complement(463..1932)
                     /gene="hutI"
                     /locus_tag="VFA35_11920"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZT56925.1"
                     /translation="MGIDEAGKRKAGYPGGIGTGSGCHVGDVATCVDGDGGVAQPAAI
                     LPQVLEGDRLERRGRCLPHPRHVSTAWRPRRRAGNLAPMPADKTTRLIANGRVWSGVA
                     AHAGVRTESVAIAEQGGKIVAVAQLDELRRRYPGAGELDAGGRLVTPGLVDCHTHIVY
                     AGNRAAEIERRLAGESYESIARAGGGIVSTVRATRAAALDALIDAALPRVDALLAEGV
                     TTLEVKSGYGLELQSELRMLQAARDLGKLRRLTVVTTYLGAHALPPERAGDSDGYVEQ
                     LCRELIPAVAVSRLADAFDAYCERMAFSADQVARAFEVARAHQLPVKLHADQLSNMHG
                     AALAARHDALSADHLEYTDVEGVAALAAAGTVAVLLPGAFHFLRESQRPPIDALRRAG
                     VPMAVATDCNPGTSPMASILTAMNFAALEFGLSTAEALNGVTAAAARALGLLPVLGTI
                     EVGKWCDLAVWNVAEPAELVQGIGMRPLHRRVWHGHPDP"
BASE COUNT          301 a          763 c          710 g          292 t
ORIGIN      
        1 gcacggcctg ctggatgcgg cgcgcgagcg gcagtccacc ctcctcagcg ccgcccgtca
       61 cggcgtcgaa gtcgatgtag acctcgtcga tgccgcggtc ctcgatgcgc ggcgcgatcg
      121 ccgcgattgc cgccttgaac agccgcgagt agcgacggta ctcgtcgaag tcggccggca
      181 acagcaccgc ccgcgggcac aggcgcgccg cgtgcatgag cgccatgccc gaacgaacgc
      241 cgaattcccg cgccgggtag gtggcggtgg tcacgacgcc gcgtccctgg taatcagcga
      301 gcagcgcaaa gccttccgcg ccttcaccgc tcacgcgcct gcgaccgccg atcaccaccg
      361 gctgggccgc gagctgcgga tagcgcagca gttccaccga cgcatagaac gcgtccatgt
      421 cgaggtgcgc gatgcgccgc gccggcgtcg ccgcgaccgg attcatggat ccgggtgtcc
      481 gtgccacact cggcgatgga gcggccgcat gccgatgccc tgcacgagct cggccggttc
      541 tgccacgttc cagacggcga gatcgcacca cttgccaacc tcgatcgtgc ccagcaccgg
      601 cagcaggccc agcgcgcgcg ccgctgcggc ggtcacgccg ttcaaggcct cggccgtgct
      661 gaggccgaat tccagcgccg cgaagttcat cgccgtcagg atcgaggcca tcggcgaggt
      721 gccgggattg cagtcggtcg cgaccgccat cggcacgccg gcgcggcgca gcgcgtcgat
      781 cggcgggcgc tggctttcgc gcaggaagtg gaacgcgccc ggcagcagca cggccaccgt
      841 tcccgcggcc gcgagcgccg cgacgccttc aacatcggtg tattcgagat gatcggccga
      901 cagcgcatcg tgtcgcgcgg cgagcgccgc gccgtgcatg ttcgacaact ggtcggcgtg
      961 cagcttgact ggcagctggt gggcgcgcgc cacttcgaac gcgcgcgcga cctggtccgc
     1021 ggagaatgcc atgcgctcgc agtaggcgtc gaaggcatcc gccaggcggc tgacggccac
     1081 cgccggaatc agctcgcggc acagctgctc aacgtagccg tcgctgtcgc cggcacgctc
     1141 cggcggcagc gcgtgcgcgc cgagataggt cgtcacgacg gtcaggcgcc ggagcttgcc
     1201 gagatcgcgc gctgcctgca gcatgcgcag ctccgactgc agctcgagcc cgtaacccga
     1261 cttgacctcg agcgtcgtca cgccctcggc gagcagcgca tcgacgcgcg gcagcgctgc
     1321 atcgatcagt gcatccaggg ccgccgcgcg cgtggcccgt acggtcgaca cgattccacc
     1381 gccggcgcgc gcgatgcttt cgtagctctc gccagcgagc cggcgctcga tctcggcggc
     1441 gcggttgccc gcgtatacga tgtgcgtgtg gcagtcgacg aggcccgggg tcacgagccg
     1501 cccgccggca tccagctccc cggcccccgg gtaacggcgc cgcagctcat cgagctgcgc
     1561 cacggcgacg atcttgccgc cctgctcggc gatcgccacc gattccgttc gcacgcctgc
     1621 gtgcgccgca acgccgctcc agacccggcc gttggcgatc aggcgggtgg ttttatcggc
     1681 gggcatgggc gcgagattac ccgcccggcg ccgcgggcgc cacgcggtgc taacatgtcg
     1741 gggatgcggc aggcatcggc cacggcgctc aagacggtca ccttcgagaa cctgtggcag
     1801 gatggccgct ggctgcgcaa cgccaccgtc gccatcgacg cacgtggcca cgtcgccgac
     1861 gtgacagccg ctgcccgtac cgatgccgcc ggggtacccg gctttacgct tcccggcctc
     1921 gtcgatgccc attgccacgc cttccagcgg ccgctcggcg cctggacgca gcgcgccgcc
     1981 gcagcgcgcg atgatttctg gagctggcgc gaagccatgt acgcgctggc acggctgctc
     2041 gatcgcgacg gactcgaggc catcag
//
LOCUS       DASRDG010000711         3249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_685298, whole genome shotgun sequence.
ACCESSION   DASRDG010000711 DASRDG010000000
VERSION     DASRDG010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685298"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VFA35_11925"
     CDS             complement(<1..739)
                     /locus_tag="VFA35_11925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZT56926.1"
                     /translation="MAGCPVCSSARTRRRWRGLRDRLFATTDERFDVDCCDDCGAHFL
                     AEIPPLERLARYYPDDYWVGPSDRQPGRTAQGGLLELYRRFVLRDHVRFVGRVLADQR
                     ARGLPVRVLDVGCGDGSFLEALGERAATGMDLSLPALRAVRARGFAAVRGTLTDCPLR
                     PGSFSLVTAFHFLEHVHPVEPVLAAMRALLAPGGEIVVQVPNCDSWQAKLLGRFWAGY
                     DVPRHLVDYSARTLCGVLERCGFAVVAT"
     gene            843..1871
                     /locus_tag="VFA35_11930"
     CDS             843..1871
                     /locus_tag="VFA35_11930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZT56927.1"
                     /translation="MPSPPRASAVVVNWNGGDMLDECIASLCAQTVSDLEIIVVDNGS
                     RDGSAARCKERFGERIVLLQNPRNEGFARANNQAFAIARGEWIFLLNNDAVAEPGAVE
                     ALLAFVQDKPDVGTLACRVNRYEQPNVFDSAGLLLYPDGVCRPRGWQEKDLGQYDRPE
                     MVLAPHGCACAYRKAMLAEVGAFDETYFCYLEDLDLGMRAELQGWKCWYVPDSRVRHH
                     KSATAGNYSKFKAYHVERNRIWNAVKLLPRMILAMSPLFTVNRYLMQFYAARTHTGLS
                     SDFVKEYSWFGLLWILLRAYCAALWRLPRMIRERHRLSKRRKISTREWYDLISRYKLD
                     AIELALKF"
     gene            complement(2015..2410)
                     /locus_tag="VFA35_11935"
     CDS             complement(2015..2410)
                     /locus_tag="VFA35_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56928.1"
                     /translation="MKPRRIAAAALVAAVACRSAVEETAMAGHGKDRAAITAEVQSRH
                     GVANQAGVPERWLGKATWRVFQLGDRQLLLAQVTETTWGNAPRAAFYGWLGDGAEPSL
                     WHDAFAFETATDGASNSTRPSRPGGSWSP"
     gene            2656..>3249
                     /locus_tag="VFA35_11940"
     CDS             2656..>3249
                     /locus_tag="VFA35_11940"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZT56929.1"
                     /translation="MIRDMRAITAACVAVALVLTKAQAQATGGVPRFDHVVVVIEENH
                     AYSQIIGSPAASYINSLAAGGALMTQSHALTHPSQPNYIALFSGSTQGVTTDSVYPHS
                     QFTAPNLGSKLLSAGFTFSGFSETMPSVGFDGASAGTAPAEYRRKHNPWVNWQDAGVP
                     LPANKLPPAVNLPYAGYFPAATAYDTLPTLSIVVPNQL"
BASE COUNT          512 a         1190 c         1086 g          461 t
ORIGIN      
        1 tcgtcgccac gacggcgaag ccgcagcgtt cgaggacgcc gcacagcgta cgcgcgctgt
       61 agtcgacgag gtgccgcggc acgtcgtagc cggcccagaa gcggccgagc agcttcgcct
      121 gccagctgtc gcagttcggc acctgcacga cgatctcgcc gcccggcgcg agcagcgcgc
      181 gcatcgccgc gagcaccggt tcgaccgggt gcacgtgctc gaggaagtgg aacgcggtga
      241 ccagcgagaa cgagcccggc cgcagcgggc agtcggtcag cgtgccgcgc accgccgcga
      301 agccgcgcgc gcgcaccgcg cgcagcgccg gcagcgacag gtccatcccg gtcgccgcgc
      361 gttcgccgag cgcttcgagg aacgacccgt cgccgcagcc gacgtcgagc acgcgcaccg
      421 gcagcccgcg cgcgcgctgg tccgcgagca cgcggccgac gaagcgcacg tggtcgcgca
      481 gcacgaagcg gcggtacagc tcgagcagcc cgccctgcgc ggtgcggccc ggctgccggt
      541 cgctcggccc gacccagtag tcgtcggggt agtagcgcgc gagccgttcg agcggcggga
      601 tctcggcgag gaagtgcgcg ccgcagtcgt cgcagcagtc gacgtcgaag cgctcgtcgg
      661 tggtcgcgaa caggcggtcg cgcagtccgc gccagcgccg gcgggtgcgc gcagacgaac
      721 agacgggaca gccggccatc gacgcgccgc actctaggaa agcgccgcgc gcgagtccaa
      781 cgcgcgcggg cgccgcgcag tcggtaagct cccgcgccct cgcccgccga tacccgagag
      841 ccatgccgag cccgccccgc gccagcgccg tcgtcgtaaa ctggaacggc ggcgacatgc
      901 tcgacgagtg catcgcctcg ctgtgcgcgc agacggtctc cgacctcgag atcatcgtcg
      961 tcgacaacgg cagccgcgac ggcagcgccg cgcgctgcaa ggaacggttc ggcgagcgca
     1021 tcgtgctgct gcagaacccg cgcaacgaag gcttcgcgcg cgccaacaac caggcgttcg
     1081 cgatcgcgcg cggcgagtgg atcttcctgc tcaacaacga cgcggtcgcc gagcccggcg
     1141 cggtcgaggc cctgctcgca ttcgtgcagg acaagcctga cgtcggcacg ctcgcgtgcc
     1201 gcgtcaaccg ctacgagcag ccgaacgtgt tcgactccgc ggggctgctg ctctaccccg
     1261 acggcgtctg ccggccgcgc ggctggcagg agaaggacct cggccagtac gaccggcccg
     1321 agatggtact ggcgccgcac ggctgcgcct gcgcctaccg caaggcgatg ctcgccgagg
     1381 tcggcgcgtt cgacgagacg tacttctgct acctcgagga cctcgacctc ggcatgcgcg
     1441 cggagctgca gggctggaag tgctggtacg tgccggattc gcgcgtccgc caccacaagt
     1501 cggcgaccgc gggcaactac tcgaagttca aggcctacca cgtcgagcgc aaccgcatct
     1561 ggaacgcggt caagctgctg ccgcggatga tcctggcgat gagcccgctg ttcaccgtca
     1621 accgctacct gatgcagttc tatgccgcgc gcacgcacac cggcctgtcg agcgacttcg
     1681 tcaaggagta ctcgtggttc ggcctgctgt ggatcctgct gcgcgcgtac tgcgcggcgc
     1741 tctggcggct gcccaggatg atccgcgaac ggcaccggct gtcgaagcgg cgcaagatca
     1801 gcacgcgcga gtggtacgac ctgatcagcc gctacaagct cgacgcgatc gagctggcgc
     1861 tgaagttctg acctgaccgc gcacccacgg cgcgccgttg cggtgctgat ccgggtcgcg
     1921 cttccgccgt cgagccgcgt cgacgcccgc taccgcttcg cttgtgccga cagatccttc
     1981 gtgaacagcc ggcgcgacgc ggcgccctct ccgatcacgg tgaccacgaa ccgcccggcc
     2041 tcgatggacg tgtggagttg ctcgcgccgt cggtagccgt ctcgaaggcg aaggcgtcgt
     2101 gccacaggga cggctcggcg ccgtcgccga gccagccgta gaaggcggcg cgcggcgcgt
     2161 tcccccaggt cgtctccgtc acttgcgcaa gcaggagctg acggtcgccg agctggaaca
     2221 ccctccacgt cgccttcccg agccaacgct ccggtacgcc cgcctggttg gccactccgt
     2281 ggcgcgactg gacttccgcg gtgatcgccg cgcgatcctt gccatgccct gccatcgccg
     2341 tctcttcgac ggcactgcgg caggcgacag cggccaccag ggccgctgcc gcgatcctcc
     2401 tcggcttcac ttcttgcctc cgcccccggc gccgaaaacg tggacttttc cgagccactt
     2461 cccgaagtgc aggatctgca gggcgttcat tgccccttgt tagcagccgc tggcacgcca
     2521 gcgaagtgca atatcgagct gcgacgacac ggaccgcgtg gagaaggcag caactgccgg
     2581 agaaggcaag gaaactgctc gaccggcagc ggcgctgcgc ggttgccccg cttggcttct
     2641 ccaggctcgg gcggcatgat ccgggacatg cgagctatca cggctgcgtg cgttgccgtt
     2701 gctctcgtcc tcaccaaggc gcaggcgcag gccaccggcg gcgtgccgcg cttcgaccac
     2761 gtggtggtcg tgatcgagga gaaccacgcc tactcacaga tcatcgggtc gcctgccgcc
     2821 tcttacatca actcgctggc tgccggcggc gccctgatga cgcagagtca tgccctcacg
     2881 cacccgagcc agcccaacta tatcgcgctc ttctcgggct cgacccaggg cgtcaccacc
     2941 gacagcgtgt acccgcacag ccagttcacc gcgccgaacc tggggtccaa gctgctgtca
     3001 gcaggcttca cgttcagcgg attctccgag acgatgccgt cggtcggctt cgacggggcc
     3061 agtgcgggga cggcgccggc cgagtaccgg cggaagcaca atccctgggt caactggcag
     3121 gacgccgggg tcccgctgcc ggcgaacaaa ctgccgccgg cggtcaatct gccctatgcc
     3181 ggctacttcc ccgccgccac cgcttacgac accctgccga cgctgagcat cgtcgtcccg
     3241 aaccagctc
//
LOCUS       DASRDG010000712         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1374921, whole genome shotgun sequence.
ACCESSION   DASRDG010000712 DASRDG010000000
VERSION     DASRDG010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374921"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFA35_11945"
     CDS             <1..789
                     /locus_tag="VFA35_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZT56930.1"
                     /translation="AGLVSNVRANSESVATASSQISQGANDLSARTEEQASALQQTAA
                     SMEQLGATVRQNADNAKQANQLALGASTVAVKGGEVVSQVVDTMKGINDSSKKIADII
                     GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAEAAKEIKGLISA
                     SVERVEQGSSLVDQAGVTMTEIVGAIRRVTDIMAEISSASVEQSAGVSQVGEAVTQMD
                     KTTQQNAALVEESAAAAESLKMQAQHMVQAVSVFKLAHGEAVAA"
     gene            complement(817..>2367)
                     /locus_tag="VFA35_11950"
     CDS             complement(817..>2367)
                     /locus_tag="VFA35_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZT56931.1"
                     /translation="LGFAVHYAFVLLLAGIVDSALPAPSVWPALFGVGVGLTLLMGFG
                     LPPVLQLARVPPLRVIRRDVGALKPASIAVLAAGAIGFAALLLAVASDLKMGLIAVGG
                     FAGAIAVFALLSWLAVSVLRRAVPEARAPRWLVLATRQIAARPAFAVLQVSALSVGLL
                     ALMLLVLLRTDLIDSWRQATPKDAPNRFVINVQPDQADAFRQRLESAGVHHYDWFPMI
                     RGRLIAINGRAVRPEDYREERAQRLLDREFNLSFDADLPPHNQLAGGRWTRNEADAVS
                     VEAGLAQTLGLKLGDSLRFDVGGTPAQGRITSLRKVDWSSMRVNFFVMFPTATLADLP
                     QSYIAAYRAPEGPGDRHDASAFDNALARDFPNITSIDVSASIAQIQKVLDQVVRAVEF
                     LFGFTLAAGLIVLFAAVAATREARAREFAVMRALGASGKLLAQVQRAELLGVGALAGV
                     LASAAAMAVGWALARYVFEFSWSASPWVPLAGGAAGALLALAAGWWGLREVLRRPVVQ
                     TLRQAADV"
BASE COUNT          400 a          846 c          863 g          258 t
ORIGIN      
        1 gccggcctcg tgagcaacgt gcgcgccaat tccgagagcg tggccaccgc gtcgtcgcag
       61 atctcgcagg gcgccaacga cctcagcgcg cgcaccgagg aacaggcctc ggcgctgcag
      121 cagaccgcgg cgtcgatgga gcagctcggc gccaccgtgc gccagaacgc cgacaacgcg
      181 aagcaggcga accagctcgc gctcggcgca tcgaccgtcg cggtcaaggg cggcgaggtc
      241 gtcagccagg tcgtcgacac gatgaagggc atcaacgaca gctcgaagaa gatcgccgac
      301 atcatcggcg tgatcgacgg catcgcgttc cagaccaaca tcctggcgct gaatgccgcg
      361 gtcgaggcgg cgcgcgccgg cgagcagggc cgcggcttcg cggtcgtcgc cagcgaggtg
      421 cgtagtctgg cgcaacgcag cgccgaagcg gcgaaagaga tcaagggcct gatctcggcg
      481 agcgtcgagc gcgtcgagca gggcagctcg ctggtcgatc aggccggcgt gacgatgacc
      541 gagatcgtcg gcgcgatccg gcgcgtgacc gacatcatgg ccgagatcag cagcgccagc
      601 gtcgagcaga gcgcgggcgt gtcgcaggtc ggcgaagcgg tgacgcagat ggacaagacc
      661 acgcagcaga acgcggcgct ggtcgaagag agcgcggccg ccgccgagag cctgaagatg
      721 caggcgcagc acatggtgca ggcggtgtcg gtgttcaagc tcgcgcacgg cgaggcggtc
      781 gccgcctgac gcggcgctgc cggccgccgg cgggtctcag acgtccgccg cctggcgcag
      841 cgtctgcacg accggccggc gcaacacctc gcgcaagccc caccagccgg ccgccagcgc
      901 gagcagtgcg ccggccgcgc cgccggccag cggcacccac ggcgatgcgc tccagctgaa
      961 ctcgaacaca tagcgcgcca gcgcccagcc gaccgccatc gcggcggccg aggcgagcac
     1021 gccggcgagc gcgccgaccc ccagcagctc ggcgcgctgc acctgcgcga gcagcttgcc
     1081 gctcgcgccg agcgcgcgca tcaccgcgaa ctcgcgcgcg cgcgcctcgc gggtcgcagc
     1141 gaccgccgcg aacagcacga tcaggcccgc agcgagcgtg aacccgaaca ggaactcgac
     1201 cgcgcgcacg acctggtcga gcaccttctg gatctgcgcg atcgaagccg agacgtcgat
     1261 gctggtgatg ttcgggaagt cgcgcgccaa ggcgttgtcg aacgcgctgg cgtcgtgtcg
     1321 gtcgcccggg ccctcgggcg cgcggtaggc ggcgatgtag ctctgcggca gatcggccag
     1381 cgtcgcggtc gggaacatca cgaagaagtt gacccgcatc gagctccagt cgaccttgcg
     1441 caggctggtg atccggccct gcgcgggggt tccgccgacg tcgaagcgca agctgtcgcc
     1501 gagcttcagc ccgagcgtct gcgcgaggcc cgcctcgacg ctgacggcgt cggcctcgtt
     1561 gcgcgtccag cggccgccgg cgagctggtt gtgcggcggc aggtcggcgt cgaagctgag
     1621 gttgaattcg cggtcgagca ggcgctgcgc gcgctcttcg cggtagtcct cgggccgcac
     1681 ggccctgccg ttgatcgcga tcagccggcc gcggatcatc gggaaccagt cgtagtggtg
     1741 cacgccggcg ctctcgaggc gctggcggaa cgcgtcggcc tggtccggct gcacgttgat
     1801 gacgaagcgg ttcggcgcgt ccttcggcgt cgcctggcgc cagctgtcga tcaggtcggt
     1861 gcgcagcagc acgagcagca tcagcgcgag caggccgacg ctgagcgccg agacctgcag
     1921 caccgcgaac gccgggcgcg ccgcgatctg gcgcgtcgcg agaacgagcc agcgcggcgc
     1981 gcgcgcctcg ggcacggcgc ggcgcagcac cgacaccgcg agccacgaca gcagcgcgaa
     2041 caccgcgatc gcacccgcga agccgccgac ggcgatcagg cccatcttca gatcgctcgc
     2101 caccgcgagc agcaaggccg cgaagccgat cgcaccggcc gcgagcacgg cgatcgaggc
     2161 cggcttgagc gcgccgacat cgcgccggat cacgcgcagc ggcggcacgc gcgcgagctg
     2221 cagcaccggc ggcaggccga agcccatcaa cagcgtcagg ccgacgccga caccgaacag
     2281 cgccggccac acgctcggcg ccggcagcgc cgaatcgacg atgccggcga gcagcagcac
     2341 gaacgcgtag tgcaccgcga agccgag
//
LOCUS       DASRDG010000713         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_114526, whole genome shotgun sequence.
ACCESSION   DASRDG010000713 DASRDG010000000
VERSION     DASRDG010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114526"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFA35_11955"
     CDS             complement(<1..305)
                     /locus_tag="VFA35_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZT56932.1"
                     /translation="MIVGRFALQRVLGEGAQASVWLGFDKRLEREVAVKLMRSDAAAD
                     PVAVGEWLREARSVSRLTHPHIVPVFEADMHGRQPYLVFEYVPGPTLAQHLKARGALP
                     "
     gene            complement(302..2137)
                     /locus_tag="VFA35_11960"
     CDS             complement(302..2137)
                     /locus_tag="VFA35_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZT56933.1"
                     /translation="MPTTAPTPIRANPTRSFGRFALRQLLGKSAATMVWLAFDPRSGA
                     EVMLTLPRVQPTDAIALEHWQRDAGLAARLHHPNLAPVAELGVQDHWPFVAVERALGV
                     TLGEWLAQHPNPKPAEAVGWVCDLLHGLAFAHEAGIAHLDLQLHSLLINERGGVSLMA
                     LCAAGDAARVARDAARARSLERTMALDPGELRARRAVCARDLLAAGVLLHRLLTGQPA
                     LDQPDTALVLARIAPLGREIVRLPWSTPLPIPEALRAIANRCTAGQERLRYQSPRTLL
                     GALSGWLDAQSEDGGGPLGLLLDRLHSVGHLPALPGLAARVARVTALESQRTDEIADQ
                     VLADMALSFELLRTLNSAQVQGTQVQGNGPVLTLRRIISLIGINGVRLAANTLRAWPG
                     PLSAPHAAALQLTFDRVRLAGHVAQALRPAGYDAQVVYLIAVLQNLGRLMLRYHFAGE
                     AEQIEQLTRPGSREPGTPEQPGLGEEAAAYAVLGIDIESLGAAVARQWGLGDDVMHMA
                     RRLPPEAPVRKPDTDAELLRLTASAANDAVDAVSTLTGARVAAALGQIAQRYARALGL
                     SVRELSDALEAGREMLRKGTAPPAQRRGDPPPVLDADLQPIPAAR"
     gene            complement(2258..>2795)
                     /locus_tag="VFA35_11965"
     CDS             complement(2258..>2795)
                     /locus_tag="VFA35_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZT56934.1"
                     /translation="VPPARLKVLRLLARPEAPARARAVPRSPDTDATRLGKAVHRALE
                     WAAASRADALTDLAHAAAAEFDAPATTVAQLAQTILQSPDCARFFSGPQLRWAGNEVP
                     IAEGGEVLRIDRLVQLVDAAGPAWWVLDYKLQHAPHQLDAYRAQLRRYREAVRRVQPG
                     EAVRCAFLTGAGEVIEID"
BASE COUNT          359 a         1014 c         1071 g          351 t
ORIGIN      
        1 ggcagcgcgc cgcgcgcctt caggtgctgc gcgagcgtcg ggccgggcac gtactcgaac
       61 acgaggtaag gctggcggcc gtgcatgtcg gcctcgaaca ccggcacgat gtgcgggtgc
      121 gtcaggcggc tcacgctgcg cgcctcgcgc agccactcgc ccaccgcgac cgggtcggcg
      181 gcggcgtcgg aacgcatcag cttgacagcg acctcgcgct cgagccgctt gtcgaagccg
      241 agccagacgc tcgcctgcgc gccctcgccg agcacgcgct gcaacgcgaa gcggccgacg
      301 atcatcgggc tgccgggatc ggctgcagat cggcgtccag caccggcggc gggtcgccgc
      361 gccgctgcgc gggcggcgcc gtgcccttgc gcagcatctc gcggccagcc tccaacgcgt
      421 cggaaagttc gcgcacgctg agcccgagcg cccgcgcgta gcgctgcgcg atctggccca
      481 gcgccgcggc cacgcgcgcg ccggtcagtg tgctgaccgc atcgaccgca tcgttggcgg
      541 cgctggccgt caggcgcagc agctcggcgt cggtgtcggg cttgcgcacc ggcgcctcgg
      601 gcggcaggcg gcgcgccatg tgcatcacgt cgtcgccgag gccccactgg cgcgcgacgg
      661 cagcgcccag cgattcgatg tcgatgccga gcaccgcgta ggccgcggcc tcttcgccca
      721 ggccgggctg ctcgggagtg cccggctcgc gcgagcccgg ccgcgtcagc tgttcgatct
      781 gctcggcctc gccggcgaag tggtagcgca gcatcagccg gccgaggttt tgcagcaccg
      841 cgatcaggta gacgacctgc gcgtcgtagc ccgcggggcg cagcgcctgg gccacgtggc
      901 cggccaggcg cacgcggtcg aaggtgagtt gcagtgccgc ggcgtgcggc gcggacagcg
      961 gcccgggcca ggcgcgcagc gtgttcgctg cgagccgcac gccgttgatg ccgatcagcg
     1021 agatgatgcg gcgcaaggtc agcaccggcc cgttgccctg gacctgcgtg ccctgcacct
     1081 gggccgagtt cagcgtgcgc agcagctcga agctgagcgc catgtcggcc agcacctggt
     1141 cggcgatctc gtcggtgcgc tggctctcca gcgccgtgac ccgggcgacg cgcgccgcga
     1201 ggcccggcag cgccggcagg tggccgacgc tgtgcaggcg gtcgagcagc aggccgagcg
     1261 ggccgccgcc gtcttccgac tgcgcgtcga gccagccgct cagcgcgccg agcagggtgc
     1321 gcgggctctg gtagcgcagc cgctcctggc ccgcggtgca gcggttcgcg atcgcgcgca
     1381 gcgcctcggg gatcggcagc ggcgtgctcc acggcagcct cacgatctcg cggccgagcg
     1441 gcgcgatgcg ggcgagcacg agggcggtat cgggctggtc gagcgccggt tggccggtca
     1501 gcaggcggtg cagcagcacg ccggcggcga gcaggtcgcg cgcgcaaacg gcgcgacgcg
     1561 cgcgcagctc gccggggtcg agcgccatcg tgcgttcgag cgaccgcgca cgcgccgcgt
     1621 cccgcgccac acgggcggcg tcgcccgcgg cgcagagcgc catcaggctg acgccgccgc
     1681 gttcgttgat cagcaggctg tgcagttgca ggtcgaggtg cgcgatgccg gcctcgtgcg
     1741 cgaatgcgag gccgtgcaac aggtcgcaga cccagccgac cgcttcggcc ggcttcgggt
     1801 tcggatgctg cgcgagccac tcgccgagcg tgacgccgag cgcgcgctcg accgcgacga
     1861 acggccagtg gtcctggacg ccgagttcgg cgaccggcgc cagattcgga tggtgcaggc
     1921 gcgcggcaag gcccgcgtcg cgctgccagt gttcgagcgc gatggcgtcg gtcggctgca
     1981 cgcgcggcag tgtcagcatc acctcggcgc cgctgcgcgg gtcgaatgcg agccagacca
     2041 tcgtggccgc gctcttgccc agcaactggc gcagcgcgaa gcggccgaac gagcgggtcg
     2101 ggttggcgcg gatcggggtc ggggcagtgg tgggcaaggc gttggcgacc atcgcaagca
     2161 ggatgaagtt gcccgattga aacccgccgc gccgcgcgcc ggtgcgcgga tgaggccgcg
     2221 catcacccgc caaatggcgg gattgcgctg cacccggtca gtcgatctcg atcacctctc
     2281 ccgcgccggt caggaacgcg cagcgcaccg cctcgccggg ctgcacgcgg cgcaccgcct
     2341 cgcggtagcg ccgcagctgc gcgcggtacg cgtcgagctg atgcggcgcg tgctggagct
     2401 tgtagtcgag cacccaccag gccgggccgg ccgcatcgac gagctgcacc agccggtcga
     2461 tgcgcaacac ctcgccgccc tcggcgatcg gcacctcgtt gccggcccag cgcagctgcg
     2521 gcccgctgaa gaaacgcgcg cagtccgggc tttgcagaat cgtctgcgcg agctgcgcga
     2581 ccgtggtggc cggggcatcg aactcggccg cggcggcgtg cgccaaatcg gtcagcgcgt
     2641 ctgcgcgcga cgccgcagcc cattcgagcg cgcggtgcac ggccttgccg agacgggtcg
     2701 cgtcggtgtc cgggctgcgc ggcacagcgc gcgcgcgcgc gggcgcctcg gggcgcgcca
     2761 gcaggcgcag cactttcagg cgcgccggcg gcacg
//
LOCUS       DASRDG010000714         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_114649, whole genome shotgun sequence.
ACCESSION   DASRDG010000714 DASRDG010000000
VERSION     DASRDG010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114649"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFA35_11970"
     CDS             <1..230
                     /locus_tag="VFA35_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56935.1"
                     /translation="EDSGFYLFGVLPIVPMQFGACVDEIVRQARLLGADGIAHVAVSY
                     RPPAFLRVVGLMLPDWFGYIGLTGSAWRLPR"
     gene            291..965
                     /locus_tag="VFA35_11975"
     CDS             291..965
                     /locus_tag="VFA35_11975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZT56936.1"
                     /translation="MAPPAPEPHPLAAIARAFVDAAGERATKQGYLAWLPRHGTVLDV
                     GCGGGAFLDVARAAGLGAIGIDCDGAAVAAARRNGHDVHLGDAVAVLRELGGTGARFH
                     GALLAHVIEHTDGEGALRLLHAIAAVLAPGAPLVVATPDVRSHIVLSELFWLDPSHVR
                     PYPRLLIERLGAAAGFDVSASFRDATTRPRRPWWRALLARLRSALSGVDKSGPQDAVV
                     VLRRRA"
     gene            complement(988..>2001)
                     /gene="eno"
                     /locus_tag="VFA35_11980"
     CDS             complement(988..>2001)
                     /gene="eno"
                     /locus_tag="VFA35_11980"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZT56937.1"
                     /translation="IDNRLIELDGSPNKGRLGANALLGVSMAIASASAAAAGRPLYEH
                     LASLVPSETRSNGLLLPVPMMNILNGGAHADNNVDFQEFMVMPVGAASFREGLRMGAE
                     VFHALKAVLKDKKLATGIGDEGGFAPNLASNSAAFEVILEAIAKAGYKAGSDLMLALD
                     AASTELFDKGKYTLEGEGKVLDRKGMVELFAGWCRTFPILSIEDGMAEDDWDGWQQLT
                     SAIGQKVQTVGDDLFVTNTERLQKGIETKAANAILIKVNQIGTLTETLEAIALAHRSG
                     MRAVMSHRSGESEDSTIADLAVACGTGQIKTGAPCRSDRVAKYNQLLRIEEQLGAAAI
                     YGM"
BASE COUNT          245 a          718 c          715 g          323 t
ORIGIN      
        1 tcgaggactc gggcttctac ctgttcggcg tgctgccgat cgtgccgatg cagttcggcg
       61 cttgcgtcga cgagatcgtg cgccaagcga gactgctcgg cgccgacggc atcgcgcacg
      121 tcgccgtctc ctaccggccg ccggcgttcc tgcgcgtcgt cggcctgatg ctgcccgact
      181 ggttcggcta catcggcctc accggcagcg cctggcggct gccgcgctga gctggaggac
      241 agtccgcttg accgcgccgc ggcgcggccg tagcagtcgg ccgctgcagc atggcgcccc
      301 ccgcacccga accgcacccg ctcgcggcga tcgcgcgcgc gttcgtcgac gcggccggtg
      361 aacgcgcgac caagcagggc tacctcgcgt ggctgccccg ccacggcacg gtgctcgacg
      421 tcggctgcgg cggcggcgcg ttcctcgacg tcgcgcgcgc ggcgggactg ggcgcgatcg
      481 gcatcgactg cgacggcgcc gcggtcgcgg cagcgcgccg gaacggccac gacgtccatc
      541 tgggcgacgc ggtcgcggtg ctgcgcgaac tgggcggcac gggcgcgcgc ttccacggcg
      601 cgctgctcgc tcacgtgatc gaacacacgg atggcgaagg cgcgctgcgg ctcctgcacg
      661 cgatcgccgc ggtgctcgcg cccggcgcgc cgctggtggt cgcgacgccc gacgtcagga
      721 gccacatcgt gctgtcggag ctgttctggc tcgacccgtc gcacgtgcga ccctacccgc
      781 gattgctgat cgaacggctc ggagctgccg cgggcttcga cgtgtctgcg tcgttccgcg
      841 acgcgacgac gcggccgcgg cggccctggt ggcgcgcgct gctcgcgcga ttgcgcagcg
      901 cgctgtcggg cgtcgacaag agcggaccgc aggatgcggt ggtggtgctg cgccggcgcg
      961 cgtgagccgc gccgcgcggc gacgcagtca catgccgtag atcgccgcgg cgccgagctg
     1021 ctcctcgatg cgcagcagct ggttgtactt cgcgacgcgg tcgctgcggc acggcgcgcc
     1081 ggtcttgatc tggccggtgc cgcaagcgac ggcgaggtcg gcgatcgtgc tgtcctcgct
     1141 ctcgccgctg cggtgcgaca tcaccgcgcg catgccgctg cggtgcgcga gcgcgatcgc
     1201 ctcgagcgtc tccgtcagcg ttccgatctg gttgaccttg atcaggatcg cgttcgcggc
     1261 cttggtctcg atgcccttct gcagccgttc ggtgttggtg acgaacaggt cgtcgccgac
     1321 cgtctgcacc ttctggccga tcgcggaggt cagctgctgc cagccgtccc agtcgtcctc
     1381 ggccatgccg tcctcgatcg acaggatcgg gaacgtacgg caccagcccg cgaacagctc
     1441 gaccatgccc ttgcgatcga gcaccttgcc ctcgccctcg agcgtgtact tgcccttgtc
     1501 gaacagctcg gtgctggcgg cgtcgagcgc gagcatcagg tcgctgccgg ccttgtagcc
     1561 ggccttggcg atcgcctcga ggatcacctc gaacgcggcc gagttgctgg cgaggttcgg
     1621 cgcgaagccg ccctcgtcgc cgatgccggt cgcgagcttc ttgtccttca gcaccgcctt
     1681 gagcgcgtgg aacacctcgg cgcccatgcg cagcccctcg cggaacgacg cggcgccgac
     1741 gggcatcacc atgaactcct ggaagtcgac gttgttgtcg gcgtgcgcgc cgccgttgag
     1801 gatgttcatc atcggcaccg gcagcagcag cccgttggac cgcgtctccg acggcacgag
     1861 cgacgccagg tgctcataga gcggccggcc cgcggccgcg gcggaggcgg acgcgatggc
     1921 catcgacacg ccgagcaggg cattggcgcc gagacggccc ttgttcggcg agccatcgag
     1981 ctcgatgagg cggttgtcga t
//
LOCUS       DASRDG010000715         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_686043, whole genome shotgun sequence.
ACCESSION   DASRDG010000715 DASRDG010000000
VERSION     DASRDG010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686043"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFA35_11985"
     CDS             complement(<1..279)
                     /locus_tag="VFA35_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZT56938.1"
                     /translation="MTADIPSPATTARAPSIWRLAGRQTARDFRAGELRLLVVAVMLA
                     VAALSAVGFFADRINGGLARDARQLLGGDAIVASDQPAPAAFVAKAREL"
     gene            397..1269
                     /locus_tag="VFA35_11990"
     CDS             397..1269
                     /locus_tag="VFA35_11990"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZT56939.1"
                     /translation="MNSSTLPPLFVSHGSPMIALEPGAAGAFMKTLGVGLDAGFGRPQ
                     AILAVSAHTAARAPVLLAAARHEAVYDFGNFDARLNELRYDAPGAPALAQRVAGLLDA
                     SGLAAHVLDEGGLDHGIWTALRYIYPNADVPILPLAFVPSQSPAQQFALGAALAPLAA
                     EGVLILGTGSITHNLRRAFAAGLRPSGTQPEIPESAAFRAWMCARSAARDWDALADYR
                     RLAPHAVDMHPTDEHLLPWFVAAGAGGRDAAPVRLHAGVELGSIGMDAYAFGPNAARL
                     AQQLAPARAGAASR"
     gene            1266..1871
                     /locus_tag="VFA35_11995"
     CDS             1266..1871
                     /locus_tag="VFA35_11995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZT56940.1"
                     /translation="MTETDLPVAAPLLLTARLRLVAADASLAAALLDFHVRNRDHLAP
                     WDPPTEPDFFTEAVQARRLRDAMAAFAAGAGFRYLLQPIGDTSRVIGSINFSSIVRGA
                     FQSCKLGYALDQQCEGQALMTEALRCAIGEMFSPRINLHRIQAAYRPENWRSAEVLKR
                     LNFHDEGVCPDYLFIDGLWRVHRLVALLNKGFVEPAAWRAG"
     gene            complement(1876..>2154)
                     /locus_tag="VFA35_12000"
     CDS             complement(1876..>2154)
                     /locus_tag="VFA35_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HZT56941.1"
                     /translation="YRAKVTVGGVPDARSGMVVDLALLRQAIEQVRGELDHRLLDDVA
                     GLGAATLENLCRFIHEQVGRPGWRVVSVEVGREASGDSCCLKVDAQVA"
BASE COUNT          287 a          817 c          767 g          283 t
ORIGIN      
        1 gagctcacgc gccttggcga cgaacgcggc cggtgccggc tggtcggacg cgacgatcgc
       61 gtcgccgccg agcagctgac gggcgtcgcg cgcgaggccg ccgttgatac ggtcggcgaa
      121 gaagccgacc gcgctcaagg cggccaccgc gagcatcacc gcgaccacca gcaagcgcag
      181 ttccccggcc cggaaatcgc gcgccgtctg gcgcccggcg agccgccaga tggagggtgc
      241 acgggcggtc gtcgcaggcg aggggatgtc ggcggtcatg agggcggaac ttacacgacg
      301 cggcgcgctc cgaccgcgcc aagggcttcg ctgcatcgaa cgttgcatgc gagcgccgcg
      361 aacgcccgca cccggcgacg gtgctgtaat gacggcatga acagttccac cctgcccccg
      421 ctcttcgtct cgcacggctc gccgatgatc gcgctcgaac ccggcgcggc cggcgccttc
      481 atgaagacgc tcggcgtggg cctcgacgcc gggttcggcc ggccacaggc gatcctcgcg
      541 gtctcggccc acaccgccgc gcgcgcgccg gtgctgctcg cggcggcgcg ccacgaggcg
      601 gtttacgact tcggcaactt cgacgcgcgg ctgaatgaat tgcgctacga cgcacccggc
      661 gcgcccgcgc tggcgcagcg cgtcgccggg ctgctcgacg cctcgggcct cgccgcgcac
      721 gtgctcgacg aaggcggcct cgaccacggc atctggaccg cgctgcgcta catctacccg
      781 aacgccgatg tgccgatcct gccgctcgcg ttcgtgccga gccagtcgcc cgcgcagcag
      841 ttcgcgctcg gcgccgcgct ggcgccgctc gcggccgaag gcgtgctgat cctgggcacc
      901 ggcagcatca cgcacaacct gcggcgcgcg ttcgccgccg ggttgcggcc gagcggcacg
      961 cagcccgaga ttcccgagag cgcggccttc cgcgcgtgga tgtgcgcgcg cagcgccgcg
     1021 cgcgactggg acgcgctggc cgactaccgc cgcctcgcgc cgcacgcggt cgacatgcac
     1081 ccgaccgacg agcacctgct gccctggttc gtcgccgccg gcgcgggcgg gcgcgacgcc
     1141 gcgccggtgc gtctgcacgc cggcgtcgag ttgggcagca tcggcatgga cgcgtatgcg
     1201 ttcggcccga acgccgcacg cctggcgcag caactcgcgc cggcgcgggc cggcgcggcc
     1261 tcgcgatgac cgagaccgac ctgccggtcg cggcgccgct gctgctgacg gcgcggctgc
     1321 ggctggtcgc cgccgacgcg tcgctcgccg cggcgctgct cgactttcac gtgcgcaacc
     1381 gcgaccacct cgcgccctgg gatccgccga ccgagcccga cttcttcacc gaggcggtgc
     1441 aggcacgccg gctgcgcgat gcgatggccg cgttcgccgc cggcgccggc tttcgctacc
     1501 tgctgcagcc gatcggcgac acgagccgcg tgatcggcag catcaacttc tcgagcatcg
     1561 tgcgcggcgc gttccagagc tgcaagctcg gctacgcgct cgaccagcag tgcgaaggcc
     1621 aggcgctgat gaccgaggcg ttgcgctgcg cgatcggcga gatgttctcg ccgcgcatca
     1681 acctgcaccg catccaggcc gcctaccggc ccgagaactg gcgcagcgcc gaggtgctga
     1741 agcggctgaa cttccacgac gagggcgtgt gccccgacta cctgttcatc gacggcctgt
     1801 ggcgcgtgca ccggctggtg gcgctgctga acaagggctt tgtcgagccg gcggcgtggc
     1861 gcgcgggctg atgcctcagg cgacctgcgc gtcgaccttc aggcaacacg aatccccgct
     1921 cgcctcgcgc ccgacctcga cgctcaccac gcgccagccg ggccggccga cctgctcgtg
     1981 gatgaagcgg cacaggttct cgagcgtcgc ggcgcccagg ccggccacgt cgtcgagcag
     2041 gcggtggtcg agctcgcccc ggacctgctc gatcgcctgg cgcagcagcg cgaggtcgac
     2101 gaccatgccg gagcgcgcat cgggcacgcc gccgacggtg accttggcgc ggta
//
LOCUS       DASRDG010000716         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_917794, whole genome shotgun sequence.
ACCESSION   DASRDG010000716 DASRDG010000000
VERSION     DASRDG010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917794"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..542
                     /locus_tag="VFA35_12005"
     CDS             57..542
                     /locus_tag="VFA35_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZT56942.1"
                     /translation="MPSFDTVCEPNLPEVKNAVENTAKEIATRFDFKGTSAAIELKDK
                     DITLIGDADFQLVQVEDILRNKLTKRNVDVRFLDKGDVQKIGGDKVKLVIKVKSGIES
                     EQSKKIQRLIKDSKLKVQAAIQGDAVRVTGAKRDDLQAAMALIRKEIADQPLSFSNFR
                     D"
     gene            545..1180
                     /locus_tag="VFA35_12010"
     CDS             545..1180
                     /locus_tag="VFA35_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HZT56943.1"
                     /translation="MRLTAALAIALLASAAAQAQSVSMAGSLGTRALLVIDGKPRQVA
                     VGTTVDGVKLISVSNNDALVEVKGQRVALQLGGSPSNLGGAASAGTGDKIVLTSVSGG
                     HFVTSGTVNGSAARFFVDTGATTITMGAADAERIGLDYKKGQLGHTTTANGVIPAYRV
                     MLTSVQIGDVQVYNVEATVLPSTMPYMLLGNSYLDRFQMRRENDRLTLEKK"
     gene            1279..2460
                     /locus_tag="VFA35_12015"
     CDS             1279..2460
                     /locus_tag="VFA35_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZT56944.1"
                     /translation="MDLNFTPDEQAFREKIHAWVKDNLPREISDKVRNSIRLSRDDMQ
                     RWAKILGKNGWLGWGWPKEFGGPGWNAIQKHLFEEETALAGAPRVVPFGPVMVAPVIM
                     AFGSPEQQRRFLPGIASGEVWWSQGYSEPGSGSDLASVKTKAERKGDYYLVNGQKTWT
                     TLGQYGDWIFCLVRTSNEGKPQTGISFLLIDMRSPGVSVRPIITMDGAHEVNEVWFDN
                     VEVPADQLIGEENKGWTYAKHLLAHERTNIADVNRAKRELERLKRIAKAEGVYDDARF
                     RDEIAKVEVGVVALEMMVLRVLSAEKSGKQSLDVAGLLKIRGSEIQQRYSELMMLAAG
                     PAAIPYIREAMEAGWQGDQSAAAGFVAPLAGTYFNMRKTTIYGGSNEVQRNIVAQTVL
                     G"
     gene            2479..3618
                     /locus_tag="VFA35_12020"
     CDS             2479..3618
                     /locus_tag="VFA35_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZT56945.1"
                     /translation="MDFDFNDDQEMLRDTVRRWVDKGYTFERRRGIVNEGGFSAAAWK
                     ELAELGLLGLQVPEENGGMGLGAIDAMVVMEELGRGIVLEPFAAVALVAVSLLKAGNA
                     PSAALWLQGIADGTERVVLAHQERGARYHLAHVETSAKNTGGTWQLNGTKTMVPAGAH
                     ASAFIVPARVGGAVDAPEGIALFLVSKGEQGVAVRGYPTQDGACAADLTLTDANAIEL
                     LPAGAAFVALEQAIDIGIAALCAEAVGAMDKLVATTVEYMNTRKQFGVAIGTFQALRH
                     RIADVKMQLELARSLSYFASLKLGEPDAARRRAIAQAKYQLGTSMRFVGQQCIQLHGG
                     IGVTDEYVAGHYFKRLTVMEMTYGDTLHQLGEVAARMKNTAGVFA"
     gene            3629..>4189
                     /locus_tag="VFA35_12025"
     CDS             3629..>4189
                     /locus_tag="VFA35_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZT56946.1"
                     /translation="MTDGRPGAARPDSITDVAGLRVGHFTDPRRPTGCTVVLTPDGAV
                     AGVDVRGAAPGTRETELLSPLNTVEQVHAILLAGGSAFGLDAAGGVMRWLDERGIGVP
                     VGPARVPIVPAAILFDLWVGDPSLRPDAAAGYAACDAASDAPVVEGNVGAGTGASVGK
                     MFGIDRAMRGGIGSASVTVDGVTVAAL"
BASE COUNT          760 a         1438 c         1413 g          578 t
ORIGIN      
        1 atcatgggag aattgtcgca ttcaccccgc gccatttgcc ctacaggagg catgacatgc
       61 cctcattcga caccgtctgc gaacccaatc tccccgaagt caagaatgcc gtggaaaaca
      121 cggccaagga aatcgccacg cgattcgatt tcaagggcac ctccgccgcc atcgagctca
      181 aggacaagga catcacgctc atcggcgatg ccgacttcca gctggtgcag gtcgaggaca
      241 tcctgcgcaa caagctcacc aagcgcaacg tggacgtgcg cttcctcgac aagggcgacg
      301 tgcagaagat cggcggcgac aaggtcaagc tggtcatcaa ggtcaagagc ggcatcgaaa
      361 gcgagcagtc caagaagatc cagcgcctga tcaaggacag caagctcaag gtgcaggccg
      421 ccatccaggg cgatgcggta cgcgtgaccg gcgccaagcg cgacgacctg caggcggcga
      481 tggcgctgat tcgcaaggaa atcgccgacc agccgctctc cttcagcaac ttcagggact
      541 agggatgcgc ctgaccgccg ccctggccat cgctctcctg gcttcggccg ccgcccaggc
      601 gcaatcggtc tcgatggccg gcagcctggg cacccgcgcc ctgctggtca tcgacggcaa
      661 gccgcgccag gtcgcagtcg gcacgacggt cgacggcgtg aagctgatca gcgtcagcaa
      721 caacgacgct ctggtcgagg tgaagggcca gcgggtcgcc ctgcagctcg gcggctcgcc
      781 cagcaacctc ggcggcgcgg ccagcgccgg caccggcgac aagatcgtcc tcacctcggt
      841 cagcggcggc cacttcgtca cctcgggcac ggtcaacggc agtgccgccc gcttcttcgt
      901 cgataccggc gccaccacca tcaccatggg cgcggccgat gccgagcgga tcggcctcga
      961 ctacaagaaa ggccagctcg gccacaccac caccgccaac ggcgtgattc cagcctaccg
     1021 ggtgatgctc acatcggtgc agatcggcga cgtccaggtc tacaacgtcg aggcgacggt
     1081 gctgccgtcg acgatgccct acatgctgct cggcaacagc tacctcgacc gcttccagat
     1141 gcggcgcgag aacgaccgtc tgaccctgga gaagaagtag ccgcccgtcg gcctctgccg
     1201 ccgaggcgac aggcattttc gaacgatcgt gctaaaaagt cgcccagagg cgcgaaccgc
     1261 acgcgaccgg agacttcgat ggacctcaat ttcacccccg acgagcaggc tttccgcgag
     1321 aagatccacg cctgggtcaa ggacaacctg ccgcgcgaga tcagcgacaa ggttcgcaac
     1381 tcgatccgcc tcagccgcga cgacatgcag cgctgggcca agatcctggg caagaacggc
     1441 tggctcggct ggggctggcc gaaggagttc ggcgggccgg gctggaacgc gatccagaag
     1501 cacctgttcg aggaagaaac cgcgctcgcc ggggcaccgc gggtcgtgcc cttcggcccg
     1561 gtgatggtgg cgccggtgat catggccttc ggctcgcccg agcagcagcg gcgcttcctg
     1621 ccgggcatcg ccagcggcga ggtctggtgg agccagggct acagcgagcc gggctcgggc
     1681 tccgacctgg cctcggtcaa aacgaaagcc gagcgcaagg gcgactacta cctcgtcaac
     1741 ggccagaaga cctggaccac gctcggccag tacggcgact ggatcttctg cctggtgcgc
     1801 accagcaacg agggcaagcc gcagaccggc atcagcttcc tgctgatcga catgaggagc
     1861 cccggcgtct cggtgcgccc gatcatcacg atggacggcg cgcacgaggt caacgaggtc
     1921 tggttcgaca atgtcgaagt gccggccgac cagctgatcg gcgaagagaa caagggctgg
     1981 acgtacgcga agcacctgct cgcgcacgaa cgcaccaaca tcgccgacgt gaaccgcgcc
     2041 aagcgcgaac tcgagcggct caagcgcatc gcgaaggccg agggcgtcta cgacgatgcg
     2101 cgcttccgcg acgagatcgc gaaggtcgag gtcggcgtgg tcgcgctcga gatgatggtg
     2161 ttgcgcgtgc tctcggccga gaagtcgggc aagcaatcgc tcgacgtcgc cggcttgctg
     2221 aagatccgcg gcagcgagat ccagcagcgc tacagcgagc tgatgatgct ggccgccggg
     2281 ccggccgcta tcccgtacat ccgcgaggcg atggaagccg gctggcaggg cgaccagtcg
     2341 gccgcggccg gcttcgtcgc gccgctcgcg ggcacgtact tcaacatgcg caagacgacg
     2401 atctacggcg gcagcaacga ggttcagcgc aacatcgtgg cccaaacggt cctcggctga
     2461 acatcacaag gaatcgacat ggacttcgac ttcaacgacg accaggaaat gctgcgcgac
     2521 acggtgcgca ggtgggtcga caagggctac accttcgagc gccgccgcgg catcgtcaat
     2581 gaaggcggct tctcggccgc cgcgtggaag gagctcgccg aactgggcct gctcggcctg
     2641 caggtccccg aagagaacgg cggcatgggc ctcggcgcga tcgacgcgat ggtcgtgatg
     2701 gaagagctcg gccgcggcat cgtgctggag ccgttcgcgg ccgtcgcgct ggtcgcggtc
     2761 agcctgctga aggccggcaa tgcgccctcg gcggcgctgt ggctgcaggg catcgccgac
     2821 ggcaccgagc gggtcgtgct cgcacaccag gagcgcggcg cgcgttacca cctcgcgcac
     2881 gtcgagacga gcgcgaagaa caccggcggc acctggcagc tgaacggcac gaagacgatg
     2941 gtgccggccg gcgcacacgc gagcgcgttc atcgtgccgg cgcgcgtcgg cggcgcggtc
     3001 gacgcgcccg aaggcatcgc cctcttcctc gtctcgaagg gcgagcaagg cgtggccgtg
     3061 cgcggctacc cgacgcagga cggcgcctgc gccgccgacc tcacgctgac cgatgccaac
     3121 gcgatcgaac tgctgcccgc gggcgcggcc ttcgttgcgc tcgaacaagc gatcgacatc
     3181 ggcatcgccg cgctctgcgc cgaagccgtg ggcgcgatgg acaagctggt cgccaccacc
     3241 gtcgagtaca tgaacacgcg caagcagttc ggcgtcgcga tcggcacgtt ccaggcgctg
     3301 cgccaccgca tcgccgacgt gaagatgcag ctcgagctcg cgcgctcgct gagctacttc
     3361 gcgagcctga agctcggcga gcccgatgcg gcgcggcgcc gcgcgatcgc gcaggcgaaa
     3421 taccagctcg gcacctcgat gcgcttcgtc ggccagcaat gcatccagct ccacggcggc
     3481 atcggcgtga ccgacgaata cgtcgccggc cactacttca agcgcctgac ggtcatggag
     3541 atgacctacg gtgacacgct gcaccagctc ggcgaagtcg cggcgcgaat gaagaacacc
     3601 gccggcgtct tcgcctgatc cgccgcacgt gaccgacggc cggcccgggg cggcgcgccc
     3661 cgactcgatc accgacgtcg ccggactgcg cgtcggccac ttcaccgacc cgcgccggcc
     3721 gaccggctgc accgtcgtcc tgacgcccga cggcgcggtc gccggcgtcg acgtgcgcgg
     3781 cgccgctccc ggcacgcgcg aaacggagct gctgtcgccg ctgaacacgg tcgagcaggt
     3841 ccacgcgatc ctgcttgccg gtggcagcgc gttcgggctc gatgccgcag gcggcgtgat
     3901 gcgctggctc gacgagcgcg gcatcggcgt gccggtcggc cctgcgcggg tgccgatcgt
     3961 gccggccgcg atcctgttcg acctgtgggt cggcgacccg tcgctccggc ccgatgccgc
     4021 ggccggctac gcggcctgcg atgcggccag cgatgcgccg gtcgtcgagg gcaatgtcgg
     4081 cgccggcacc ggcgcgtcgg tcggcaagat gttcggcatc gaccgtgcga tgcgaggcgg
     4141 gatcggcagc gcgtcggtca cggtcgacgg ggtgacagtt gccgcgctg
//
LOCUS       DASRDG010000717         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1719663, whole genome shotgun sequence.
ACCESSION   DASRDG010000717 DASRDG010000000
VERSION     DASRDG010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719663"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..>2301)
                     /locus_tag="VFA35_12030"
     CDS             complement(4..>2301)
                     /locus_tag="VFA35_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZT56947.1"
                     /translation="NGYVGNSVDAAEVRSGLDGAGAEDGFATGLMALLLGDLTACEIA
                     WTMADADRGRPLIDAGLGLLYAADGLPERAYPRLFHAAREFPAAAGLRIELADAALAM
                     DDPGPARALLADPAAGGNAALRPRLLLLAADVAARDGDVAGAERTYLELLGRDPDDDA
                     VRRRMARLAVDRGEPALAREHLERALLAAPELARLHLDLARLCLRMRDLAGYLAQARF
                     AAGRVDAAAASRGARTDCAAILRLGGLQRLSAGDEETGEGEARDAVATRDWRDLELQL
                     PQLPRGVSPEELATALRLLACFDRAADRVARVDPRPYARPLHGVYAVLAARPQLAGPL
                     PWWSLPLLFAAAGPVLREGPTLIAEYTLPFQRSLGTRLHTIAVSPIEDPEADARRRFG
                     HALAATTGDDGETWLLAGCPPAGEETPGVAIVLVGRTGEVRHVARAESAATMFGLSVA
                     WVGDLDGDGAPDYAVGAPAQKRGQNGYVRLCSGRDGRTLRTVTGAEAGFGIAVAALGD
                     VDGDGVPDLGVGASPLLRSNAPLGSAMIISGRDGSVLRTFTSDRAGIWFGAAVGNAGD
                     VDKDGVDDFVVGGNLGDASGFVCVYSGRTGALLHTFAEESPLGCFGAHVGTAGDLDRD
                     GHADVLVGAPGAQQGEQTGRALIYSGRTGALLARLQSDATGDRFGASSCVLPDASPNG
                     GPAIAIGAPFGGAAGSGFVRVFELRTQRPLQSLFGTSSCLSFGSAIADLGASDPRRRL
                     AVAAVEHTNGQGVVFRLEAVEIRRR"
BASE COUNT          285 a          885 c          876 g          255 t
ORIGIN      
        1 gactcagcgg cgtcggatct ccacggcttc gaggcgaaag acgacgccct ggccgttggt
       61 gtgctccacg gcggcgaccg ccaggcgccg gcgcggatcc gaggcgccga ggtcggcgat
      121 ggcgctgccg aacgagaggc agctcgaggt gccgaacagg ctctgcagcg gtcgctgcgt
      181 gcgcagctcg aacacgcgca cgaagccgct gccggccgcg ccgccgaacg gcgcgccgat
      241 cgcgatcgcc ggaccgccgt tcgggctcgc gtctgggagc acgcaggagc tggcgccgaa
      301 ccggtcgccg gtggcgtcgc tctgcagccg cgccagcagc gcgccggtgc ggccgctgta
      361 gatcagcgcg cgccccgtct gctcgccctg ctgcgcgccc ggcgcgccga ccagcacgtc
      421 ggcgtgaccg tcgcggtcca ggtcgccggc ggtgcccacg tgggcgccga agcagccgag
      481 cggcgactcc tcggcgaacg tgtgcagcag cgcgccggtg cggcccgagt acacgcacac
      541 gaatccgctg gcgtcgccga ggttgccgcc gacgacgaag tcgtcgaccc cgtccttgtc
      601 gacgtcgccc gcgttgccga ccgcggctcc gaaccagatg ccggcgcggt cgctcgtgaa
      661 cgtgcgcagc acgctgccgt cgcggccgga gatgatcatg gcactgccga gcggtgcgtt
      721 gctgcgcagc agcggcgagg cgccgacgcc gaggtccggc acgccgtcgc cgtcgacgtc
      781 gccgagcgcg gccaccgcga tgccgaagcc ggcctccgcg cccgtcaccg tgcgcagcgt
      841 gcgaccatcg cgacccgagc agagccggac gtagccgttc tgaccccgct tctgcgccgg
      901 cgcgccgacg gcgtagtcgg gcgcgccgtc gccgtcgaga tccccgaccc atgccaccga
      961 caggccgaac atcgtcgccg ccgactccgc gcgcgcgacg tgccgcactt cgccggtgcg
     1021 gcccacgagc acgatcgcga cgccgggcgt ctcttcgccg gccggcggac agccggcgag
     1081 cagccaggtc tcgccgtcgt cgcccgtcgt cgcggccagc gcgtgcccga aacgccggcg
     1141 cgcatcggcc tcggggtctt cgatcgggct cacggcgatg gtgtgcagcc gcgtgccgag
     1201 gctgcgctgg aacggcagcg tgtactccgc gatcagcgtc ggtccctcgc gcagcaccgg
     1261 cccagccgct gcgaacagca gcggcagcga ccaccacggc agcggcccgg ccagctgcgg
     1321 gcgcgcggcg agcaccgcgt agacgccgtg cagcggccgt gcgtacggcc gcgggtcgac
     1381 gcgcgcgacg cggtcggcgg cgcggtcgaa gcaggccagc aggcgcagcg ccgtggcgag
     1441 ttcttcggga ctcacgccgc ggggcagttg cggcagctgg agttcgaggt cgcgccagtc
     1501 gcgcgtggcg accgcgtcgc gcgcctcgcc ttcgccggtc tcctcgtcgc cggccgacag
     1561 ccgctgcagg ccgccgagcc gcaggatcgc ggcgcagtcc gtgcgcgcgc cgcgcgacgc
     1621 agcggcggcg tcgacgcgcc ccgcggcgaa gcgcgcctgg gcgaggtagc cggcgaggtc
     1681 gcgcatgcgc aggcacaggc gcgcgaggtc caggtgcagc cgcgcgagct ccggcgccgc
     1741 cagcagcgcg cgttcgaggt gctcgcgcgc cagcgccggc tcgccgcgat cgacggcgag
     1801 gcgcgccatc cgccggcgga cggcgtcgtc gtcggggtcg cggccgagca gttcgagata
     1861 ggtgcgctcg gcgccggcga cgtcgccgtc gcgcgccgcc acgtcggccg ccagcagaag
     1921 gagccgcggc cgcagcgccg cgttgccgcc cgccgccgga tcggcgagca atgcgcgcgc
     1981 ggggccgggg tcgtccatgg ccagcgccgc gtcggcgagc tcgatgcgca gcccggccgc
     2041 cgccgggaat tcgcgcgccg cgtggaacag ccgcgggtac gcgcgctcgg gcagcccgtc
     2101 ggcggcgtag agcaggccga ggcccgcgtc gatcaacggc cggccgcggt cggcatcggc
     2161 catcgtccag gcgatctcgc atgcggtcag gtcgccgagc agcagcgcca tgagcccggt
     2221 ggcgaacccg tcctcggcgc ccgcaccgtc gagacccgaa cgcacctccg ccgcgtcgac
     2281 gctgttgccg acgtagccgt t
//
LOCUS       DASRDG010000718         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1719682, whole genome shotgun sequence.
ACCESSION   DASRDG010000718 DASRDG010000000
VERSION     DASRDG010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719682"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFA35_12035"
     CDS             complement(<1..206)
                     /locus_tag="VFA35_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56948.1"
                     /translation="MACRFRRQQVKPVHVAGVGMIPFAKPGKHAPYPEMAAQAVRAAL
                     ADAGVGYDAVQQAYVGYVYGDSTAG"
     gene            349..1326
                     /locus_tag="VFA35_12040"
     CDS             349..1326
                     /locus_tag="VFA35_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZT56949.1"
                     /translation="MAFVHGMLEGVVAQGHPVDGCLAQARIPPELLAQPGARVTVRQY
                     VDLFAALIRTMGDDGLGFFSRPLRRGCMALIARSALDAPTLDAAIRRATRTFDLLQDD
                     VRARQAREGTLAGLALELTPAAPKFMHELLLRVFWRLFAWLAGGKLELARIDFAFAPP
                     AYREEYDVVFPAPLRFACGASAFWFDARQLNVPIRQDADSLRAFLRDVPSAIVMPRWS
                     VSTSTSRVRVHLQSRQPVWPDLAETATALGMSTATLQRRLVVEHTSFQKVKDELRRDV
                     AVVRLNAAHATLAEIAFELGFADSAAFQRAFKGWTGSAPGSYRQRQAGE"
     gene            complement(1433..>2105)
                     /locus_tag="VFA35_12045"
     CDS             complement(1433..>2105)
                     /locus_tag="VFA35_12045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZT56950.1"
                     /translation="DYAAHARLYVPRGFPKPNEPDHAASVALLAARCARALGGRTFVL
                     TTTLRALQAIGERLKAEFDANGDALQVLVQGQAPKRQLMQQFLAQPRSVLVGSQSFWE
                     GIDVPGDALQCVLIDKLPFPPPNDPLVEARVKRLEGEGRNPFADYFVAEAAVSLKQGA
                     GRLIRSETDRGLLVVCDPRMAGMNYGKRLREALPPMTMVATEAQALDWLATLAGAALP
                     YQPEL"
BASE COUNT          311 a          734 c          702 g          358 t
ORIGIN      
        1 cctgcagtgg aatcgccgta gacatagccg acgtaggcct gctgcaccgc gtcatagccg
       61 acaccggcat ccgccagcgc agcgcgcacg gcctgcgccg ccatctcggg gtaaggggca
      121 tgcttgccgg gcttggcaaa cgggatcatg ccgacaccgg caacatggac tggtttcacc
      181 tgctgtctcc tgaatcggca ggccatctcg ggtgcggcct gggtcgcata atggtgcagt
      241 aggttctcgc tcccatcgat tgcgcatcac gtcgacctga tggcaaatca catcacccgc
      301 ggtgtgcctc cctgttccct ggcgcttgac tcgcccgcca cgattcccat ggccttcgtg
      361 cacggcatgc tggaaggcgt cgtggcgcaa ggtcacccgg tcgacggatg tcttgcacag
      421 gcgcgcatcc ccccggaact gctggcgcag cccggtgcac gcgtgacggt gaggcagtat
      481 gtcgaccttt tcgcggcctt gatccgcacc atgggcgacg acgggctggg cttcttctcg
      541 cgcccgctgc ggcggggctg catggcgctg attgcgcgca gtgcgctcga tgcgccgacg
      601 ctggacgcgg cgatccggcg cgccacgcgc accttcgatc tgttgcagga cgatgtccgc
      661 gcacgtcagg cgcgcgaagg aacgcttgct ggcctcgcgc tggaactgac gccggccgca
      721 cccaagttca tgcacgagtt gctgctgcgg gtgttctggc gcctgttcgc ctggctcgcg
      781 ggcggcaagc tcgaactggc gcggatcgac ttcgccttcg cgccgccggc ctaccgcgaa
      841 gagtacgacg tggtgtttcc ggcgccactg cggttcgcat gcggggcatc ggccttctgg
      901 ttcgatgcgc gccaattgaa cgtgcccatc cgccaggatg ccgattcgct tcgagccttc
      961 cttcgcgatg tgccgagcgc catcgtcatg ccgcgctgga gtgtgagcac cagtacctcc
     1021 cgtgtgcgtg ttcacctgca atcgcggcag cccgtgtggc cggacctggc cgagacggca
     1081 acggccctgg gcatgtcgac ggcgacgctg caaaggcgcc tggtcgtcga acacacctcg
     1141 ttccagaaag tgaaggacga actgcgccgc gatgtcgcgg tggtccgcct caacgccgct
     1201 cacgcgacgt tggcagagat tgcgttcgaa ctgggcttcg cagacagcgc ggccttccag
     1261 cgcgcattca agggctggac gggcagcgcg ccagggtcct atcggcagcg gcaggctggc
     1321 gagtaggcgg gccctgctct tcatttttca atctcaggcg ccgtggctcg ccgcgagtca
     1381 cgtacaagcg gtcgagccct cgtcactgct gggctacggc ctgatcagag gctcaaagtt
     1441 cgggctggta cggcaacgcg gcgccggcga gcgtcgccag ccagtcgagc gcctgcgcct
     1501 cggtcgcgac catcgtcatc ggtggcagcg cctcgcgcag ccgcttgccg tagttcatgc
     1561 ccgccatgcg cgggtcgcac accaccagca ggccgcgatc ggtctcgctg cggatcagcc
     1621 gcccggcgcc ctgcttcaac gacaccgccg cctcggcgac gaagtagtcg gcaaacgggt
     1681 tgcgcccctc gccttcgagc cgcttcacgc gcgcctcgac cagcggatcg ttcggcggcg
     1741 ggaagggcag cttgtcgatc agcacgcact gcagcgcgtc gccgggcacg tcgatgcctt
     1801 cccagaagct ctgcgagccg accagcaccg agcgcggctg cgcgaggaac tgctgcatca
     1861 gctggcgctt cggggcctgg ccctgcacca gcacctgcag cgcatcgccg ttcgcgtcga
     1921 actccgcctt caggcgttca ccgatcgcct gcagcgcgcg cagcgtcgtc gtcagcacga
     1981 aggtgcggcc gccgagcgcg cgcgcgcagc gcgctgcgag cagcgcaacc gaggccgcat
     2041 gatccggctc gttcggcttc gggaagccgc gcggcacgta caggcgcgcg tgcgccgcgt
     2101 agtcg
//
LOCUS       DASRDG010000719         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1147905, whole genome shotgun sequence.
ACCESSION   DASRDG010000719 DASRDG010000000
VERSION     DASRDG010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147905"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2295)
                     /locus_tag="VFA35_12050"
     CDS             complement(<1..2295)
                     /locus_tag="VFA35_12050"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZT56951.1"
                     /translation="MNRDDRHFPPHHSPLREDIHTLGELLGSVLREQGGDELYELTEG
                     DRQSAIRRRTGAGVALELRARVQGRRPQLARDLLRAFATYFQLANVAEKVHRIRRRRQ
                     YFLADSGRPQPQGVLATLAALKDQGLGLDEVLELLQQLRIEPVLVAHPTEAARRTSLR
                     RQQRIADRLLDCSNPLLAPQEKRTLLERIRADVTIDWQTAEHPRELVTVADEREHVIF
                     HFAEVLYRIVPVFYEELALALARLYGSDPESLGLPLLVRFGSWVGGDMDGAADVHAKS
                     IRETLARQQQVIINKYFDECQALAQQLSQSALRVAVSEAMSRRSEEYATLLPGAPGAT
                     PTRHDRMPYRIFFGQIGERLRNTLDGRPNGYDRPAQFRNDVGMVAESLRAHRGSHAGC
                     FLVRRLLARIDTFGFHLATLDLRQHASVHDAVLAQGLDDPQWAQRDAGERQRRLVEVL
                     ANDTGPRRTFDALGRRTLAVFEAVTQGRHRYGPDAVGLYIVSGAATAADVLAPLVLAH
                     WAGARDRHNGTAAIDVAPQFDSVPTLDGCGEVMRALLAEPVYQRHLAARARTQTVLIG
                     YSESNRESGMVASRLAAYRAQRTLAAALDSAHDRHLLFYSRGGSIPRGGGRIDALLRA
                     APPESISGVLRFTEQGEGIAQNFGLRANAMRTLERAFHALALATQAVRRGATSRESPE
                     SEELALQLSAASRVAWRALLEGPGFFEFFRAATPIDVIERMRIGAVHRLEPQEAPRIE
                     AVPAAAWVYAWSQSRHMLPGWYGAG"
     gene            complement(2359..>2781)
                     /locus_tag="VFA35_12055"
     CDS             complement(2359..>2781)
                     /locus_tag="VFA35_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZT56952.1"
                     /translation="GATDVVPETLEASLMLVVQVLLLLKLPASKVMRTVAEIRADRYA
                     TLRSVFRHEGGRVVDETHSFREELRSILLPPGAWAVGRSIEEVRGQGADVSFAAVRRH
                     GITGRQPEPGTVLREGDIVVIDGTPEALEHAEAVLLGG"
BASE COUNT          387 a          999 c          994 g          401 t
ORIGIN      
        1 tccggcgcca taccagcccg gcagcatgtg gcgcgactgc gaccaggcgt agacccaggc
       61 cgccgccggc accgcctcga tgcgcggggc ttcctgcggc tcgaggcgat gcacggcgcc
      121 gatgcgcatc cgctcgatca cgtcgatcgg cgtcgcggcg cggaagaact cgaagaagcc
      181 cggaccctcg agcagtgcgc gccaggccac gcggctcgcc gcggatagct gcagcgccag
      241 ctcctcggac tcggggctct cgcggctggt ggcgccgcgc cggacagcct gggtcgccag
      301 cgccagcgcg tggaaggcac gctcgagcgt gcgcattgcg ttggcgcgca agccgaagtt
      361 ctgcgcgatg ccctcgccct gctcggtgaa acgcagcacc ccgctgatcg actcgggtgg
      421 cgccgcgcgc aacaatgcgt cgatgcgccc gccgccacgc ggaatgctgc cgccgcgact
      481 gtagaacagc aggtgccggt catgggcgct gtcgagcgcg gccgcgagcg tgcgctgcgc
      541 acggtaggcg gccaggcgcg aggccaccat gccgctctcg cggttgctct cggagtaacc
      601 gatcagcacc gtctgcgtgc gtgcgcgtgc cgccaggtgc cgctggtaca caggttcggc
      661 gagcagcgcc cgcatgactt cgccgcagcc atcgagcgtc ggcaccgagt cgaattgcgg
      721 cgcgacgtcg atcgcggcgg tgccgttgtg gcggtcgcgg gcgccggccc agtgcgccag
      781 caccagcggc gccagcacgt cggcggcggt agcggcgccg ctgacgatgt acaagccgac
      841 cgcgtcggga ccgtagcggt ggcgtccctg cgtgacggcc tcgaacactg cgagcgtgcg
      901 ccggccgagg gcgtcgaaag tgcgccgcgg cccggtgtcg ttcgccagca cttcgaccag
      961 tcgccgctga cgctcgccgg catcgcgctg cgcccactgc gggtcgtcca ggccctgcgc
     1021 cagcaccgcg tcgtggacgc tggcgtgctg gcgcaggtcg agcgttgcga ggtggaagcc
     1081 gaaagtgtcg atgcgcgcga gcaggcgccg caccaggaag cagccggcgt gcgagccgcg
     1141 atgcgcacgc aggctctccg ccaccatgcc gacgtcgttg cggaattgcg ccggccggtc
     1201 gtacccgttc gggcgtccgt cgagcgtgtt gcgcaatcgt tcgccgatct ggccaaagaa
     1261 aatccggtac ggcatgcgat cgtggcgcgt gggcgtggcg ccgggcgcgc cgggcaggag
     1321 cgtcgcgtat tcctcgctgc gccggctcat cgcctccgac accgccacgc gcagcgcgct
     1381 ttgcgacagc tgctgcgcca gcgcctggca ctcatcgaag tacttgttga tgatgacctg
     1441 ctgctggcgc gcgagcgtct cgcggatgct cttggcgtgg acgtcggcgg cgccgtccat
     1501 gtcgccgcca acccaggagc cgaagcgcac cagcagcggc agtccgagcg actcggggtc
     1561 ggagccgtac agccgcgcca gcgccagcgc gagttcctcg tagaacaccg gcacgatgcg
     1621 gtacagcacc tcggcgaaat ggaagatcac gtgctcgcgc tcgtctgcaa cggtcacgag
     1681 ctcgcgcgga tgctccgcgg tctgccagtc gatcgtcacg tcggcgcgga tgcgctcgag
     1741 aagcgtgcgc ttctcctgcg gcgcgagcag cggattgctg cagtcgagca ggcgatcggc
     1801 gatacgctgc tggcggcgca ggctcgtgcg ccgcgccgcc tcggtcgggt gcgcgaccag
     1861 caccggctcg atgcgcagct gctgcagcag ctccagcacc tcgtcgagcc ccagcccctg
     1921 gtccttgagc gccgccagcg tggccagcac tccctgcggc tgcggccggc cgctgtcggc
     1981 gaggaagtac tggcggcggc gccggatgcg gtgcaccttc tccgccacgt tggcgagctg
     2041 gaagtaagtc gcgaaggcac gcaacaggtc gcgcgccaac tgcggacgac gtccctgcac
     2101 gcgcgcgcgc agctcgagcg ccacgcccgc gccggtgcgg cggcgtatgg cactctgccg
     2161 gtcgccctcg gtcagctcgt agagttcatc gcccccctgc tcgcgcagca cgctgccgag
     2221 caactcgccc agggtgtgga tgtcctcgcg cagtgggctg tggtgcggcg gaaaatggcg
     2281 atcgtcgcgg ttcatgtcgc ctggtttcgg cgcccgttgc gggccgcgca ttgtacccgc
     2341 aagcgccggc cgcgggacct acccgcccag cagcacggcc tcggcgtgct cgagcgcctc
     2401 gggcgtgcca tcgatcacca cgatatcgcc ttcacgcaac acggtgccgg gctcgggctg
     2461 gcggccggtg atgccgtggc ggcgcaccgc ggcgaagctg acgtcggcgc cctgcccgcg
     2521 cacctcctcg atgctgcggc cgaccgccca ggccccgggt ggcagcagga tcgagcgcag
     2581 ctcctcgcgg aacgagtgcg tctcgtctac gacccgtccg ccctcgtgcc ggaacacgct
     2641 gcgcagcgtt gcgtaacgat cggcgcggat ctcggcgacg gtgcgcatca ccttcgacgc
     2701 cggcagcttc agcaatagca gtacctgtac cacgagcatc aggctggcct cgagcgtctc
     2761 gggcacgacg tcggtggcgc c
//
LOCUS       DASRDG010000720         5326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_570099, whole genome shotgun sequence.
ACCESSION   DASRDG010000720 DASRDG010000000
VERSION     DASRDG010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570099"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFA35_12060"
     CDS             complement(<1..418)
                     /locus_tag="VFA35_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZT56953.1"
                     /translation="MSQPQVKPIPDGMHTITPHLVCAGAADAIGFYAKAFGAVEQGRV
                     PGPDGRLMHAQVKIGDSMLMLVDEMPEWGALGPKALNGTPVTIHLYVKDVDAAFAQAV
                     AAGATAKMPPADMFWGDRYGQVEDPFGHRWSIATHTR"
     gene            630..2093
                     /gene="zwf"
                     /locus_tag="VFA35_12065"
     CDS             630..2093
                     /gene="zwf"
                     /locus_tag="VFA35_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZT56954.1"
                     /translation="MAKIPPGVGPRFAQVVVLFGATGDLARRKLLPGLYHLASAGFIP
                     GCRIIGVSLDEISVDDFRRFARSALDEFSSRKITDRDWNTFAASLDYVPLSAGPAALR
                     AAVDAAEASLGGETRRLHYLSVPPSAALSAVRLLGDAGLVERSRIVMEKPFGTDKASA
                     VALNAKLHEVFAEAQIFRIDHFLGKEPAQNILAFRFANGLFEPIWNRNFIDHVQIDVP
                     ETLGLGKRSGFYEQTGAYRDMVVTHLFQILAFMAMEPPTALEPAPISEEKNKVFRSML
                     PIQPHDVVRGQYTGYRGEEGVDRESETETFIALKCFIDNWRWAGVPFFLRTGKRLAEG
                     QRIISIAFREPPKSMFPAGSGVGAQGPDHLTFDLADASKMSLSFYGKRPGPGMRLDKL
                     SLQFAMHDTGLIGDVLEAYERLILDAMRGDRTLFTTAEGIERLWEVSTHLLEAPPPVR
                     LYAPGSWGPKSVHQLIAPHAWRLPFERAWRDPNKAGA"
     gene            complement(2103..3080)
                     /locus_tag="VFA35_12070"
     CDS             complement(2103..3080)
                     /locus_tag="VFA35_12070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZT56955.1"
                     /translation="MTPPLSVLDLSPITQGSDAAQSFRNTLDLAQHAEAWGFKRFWLA
                     EHHGMPGIASAATAVLIGFVAGGTKTIRVGAGGIMLPNHSPLVIAEQFGTLESLYPGR
                     IDLGLGRAPGSDQVTARAMRRNLASDADEFPQDVLELADYFSAEPRQPVRAVPGRGLN
                     VPLWILGSSLFGAQLAAALGLPYAFASHFAPAQMMQAVALYRQQFKPSAQLAKPYVML
                     GFNVFAADSDEEAEFRATSMQQAFVNLRSGRPAQLPPPKAGYRESLGPPESALLESVL
                     SCAAIGAPDTVRRALRAFTERTGADELMVTSQIFDHAARLRSYEITAGL"
     gene            complement(3090..5321)
                     /locus_tag="VFA35_12075"
                     /pseudo
     CDS             complement(3090..5321)
                     /locus_tag="VFA35_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829730.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT          843 a         1845 c         1841 g          797 t
ORIGIN      
        1 cgcgcgtgtg ggtcgcgatc gaccagcggt ggccgaacgg gtcttcgacc tggccgtagc
       61 ggtcgcccca gaacatgtct gccggcggca tcttcgcggt ggcgccggct gcaaccgcct
      121 gcgcgaacgc cgcatcgaca tccttcacgt acagatggat cgtgaccggc gtgccgttca
      181 gcgccttcgg gccgagcgcg ccccactcgg gcatctcgtc gaccagcatc agcatcgagt
      241 cgccgatctt cacttgtgcg tgcatcagtc ggccgtccgg gcccggcacg cgaccctgct
      301 cgacggcacc gaaggccttc gcgtagaacc cgatcgcgtc ggcggcgccg gcacagacga
      361 ggtgcggcgt gatcgtgtgc atgccgtcgg ggatgggctt gacttggggc tggctcatgg
      421 tgggctcctt gggtggcgaa ggcgagaggt tgaatgagac ctcgccggtt cgcaaggcct
      481 ccaccctgat gacgggcggg tcgccgcgaa atcgacaggc cgagcccgaa aaatcaccgc
      541 ctcgccaccg gcccgactgg cccgcgtctt gcacgagaat ccaccgaccg gcgcgcgacg
      601 ccccggcggc agaagaagag gacgtgcaca tggccaagat ccctcccggc gtgggcccgc
      661 ggtttgcgca ggtcgtggtg ctgttcggtg cgaccggcga tctggcgcgg cgcaagctgc
      721 tgccggggct gtaccacctc gccagcgccg gcttcatccc gggctgccgg atcatcggcg
      781 tctcgctcga cgagatcagc gtcgacgact tccgtcgatt cgcccgcagc gcgctcgacg
      841 agttttcgag ccgcaagatc accgaccgcg actggaacac cttcgccgcc agcctcgact
      901 acgtgccgct ctccgccggc ccggcggcgt tgcgcgcggc ggtcgatgcc gccgaggctt
      961 cgctcggcgg ggaaacccgc cgcctgcact acctgagcgt gccgccgagc gcggcgttgt
     1021 cggcggtgcg cctgctgggc gacgccggcc tcgtcgaacg ctcgcgcatc gtgatggaaa
     1081 agcccttcgg caccgacaag gccagcgcgg tcgcgctgaa cgcgaagctg cacgaggtgt
     1141 tcgccgaggc gcagatcttc cgcatcgacc actttctcgg caaggaaccg gcgcagaaca
     1201 tcctcgcgtt ccgcttcgcg aacggcctgt tcgagccgat ctggaaccgg aatttcatcg
     1261 accacgtgca gatcgacgtg cccgagacgc tcggcctggg caagcgctcg ggcttttacg
     1321 agcagaccgg cgcgtaccgc gacatggtgg tgacgcacct gttccagatc ctcgcgttca
     1381 tggcgatgga gccgccgacc gcgctcgagc cggcgccgat cagcgaggag aagaacaagg
     1441 tcttccgcag catgctgccg atccagccgc acgacgtggt gcgcggccag tacaccggct
     1501 accgcggcga ggagggtgtc gaccgcgaat ccgagaccga gaccttcatc gcgctgaagt
     1561 gcttcatcga caactggcgc tgggccggcg tgccgttctt cctgcgcacc ggcaagcgcc
     1621 tggccgaggg ccagcgcatc atctcgatcg cgttccgcga gccgccgaag agcatgttcc
     1681 cggccggctc gggcgtcggc gcacaagggc ccgatcacct gaccttcgac ctcgccgatg
     1741 cgtcgaagat gagcctgtcg ttctacggca agcgccccgg cccgggcatg cgcctggaca
     1801 agctgagcct gcagttcgcg atgcacgaca ccgggctgat cggcgacgtg ctcgaagcct
     1861 acgagcgcct gatcctcgac gcgatgcgcg gcgaccgcac cctgttcacc accgccgaag
     1921 gcatcgagcg actctgggaa gtgtcgactc acttgctcga agcgccgccg ccggtgcggc
     1981 tgtatgcgcc gggctcgtgg gggccgaagt cggtccacca gctgatcgcg ccgcacgcgt
     2041 ggcggctgcc gttcgagcgc gcctggcgcg atccgaacaa ggcgggcgcg tgatccgccg
     2101 gcctacagcc cggccgtgat ttcgtacgag cgcagccgcg cggcgtggtc gaagatctgc
     2161 gaggtgacca tcagctcgtc ggcaccggtg cgctcggtga aggcgcgcag cgcgcgacgc
     2221 accgtgtcgg gcgcgccgat cgcggcgcac gagagcaccg attcgagcag cgcgctctcg
     2281 ggcgggccca ggctttcgcg gtagccggcc ttcggcggtg gcagctgggc cggccggccg
     2341 ctgcgcaggt tcacgaaggc ctgctgcatc gaggtggcgc gaaactcggc ttcctcgtcg
     2401 gagtcggccg cgaacacgtt gaagccgagc atcacgtagg gcttggcgag ttgcgccgag
     2461 ggcttgaact gctgccggta cagcgcgacg gcctgcatca tctgcgccgg cgcgaagtgc
     2521 gacgcgaacg catacggcag cccgagcgcg gcggcaagct gcgcgccgaa caggctggag
     2581 ccgaggatcc acagcggcac attcagcccg cggcccggca ccgcccgcac cggctggcgc
     2641 ggctcggccg agaagtagtc ggcgagctcc agcacgtcct gcgggaattc gtcggcgtcc
     2701 gacgcgaggt tgcggcgcat cgcgcgcgcg gtgacctggt cggagccggg cgcgcggccg
     2761 agaccgaggt cgatgcgccc ggggtacagc gattcgagcg tgccgaactg ctcggcgatc
     2821 accagcggcg agtggttcgg cagcatgatg ccgccggcac cgacgcggat cgtcttcgtg
     2881 ccgccggcca caaagccgat cagcaccgcg gtcgcggcgc tcgcgatgcc tggcatgccg
     2941 tggtgctcgg cgagccagaa gcgcttgaag ccccaggcct cggcgtgctg cgccagatcg
     3001 agcgtgttgc ggaacgattg cgcggcgtcg ctgccctgcg tgatcggcga caggtcgagg
     3061 acggacagcg gtggggtcat ctcggtcttt cagacgatgt cggccgccgg cgcggcgcgg
     3121 cggtgcgtga tcttgcgcgt ccattgcacc acgtcgtcga cgaaggtgaa cagcaccggg
     3181 atcacgagca ggctcaggaa ggtgctggtg atcagcccgc cgatcacgac gatcgccatc
     3241 ggcgcgcgaa aactcgggtc ggtgcccagg ccgagcgcga tcggcatcat gccggcgccc
     3301 attgcgatcg tcgtcatcac gatcgggcgg gcgcgcttgt ggcaggcgtc gagcagcgcc
     3361 tcggtgcgcg tcatgccccg atcgcgccgc gccatgatcg cgtactcgat cagcaggatc
     3421 gagttcttgg tcgcgatgcc catcagcatg atcagcccga tcatcgacgg catcgacagc
     3481 gccgtgcgcg tgatgaacag cgccagcaac gcgcccggaa tcgacagcac cagcgccgag
     3541 agaatcgtca ccggctgcac gaagtcgtgg aacagcagca ccagcacgat gtagatgcac
     3601 aggaccccgg tcagcatcgc caggccgaag ctcgcgaaca gctcgcccat cgcttcggcg
     3661 tcgccgacgg tggcctgcga gatgccgggc ggcaggttct tgagcgcggg cagtgcgagc
     3721 accttcttct cgacctcgcc gagcggctgg ccgttcagct cgatctcgaa gttgatgttg
     3781 cgctggcggt cgtagcggtc gatctgcgcc gggccgctgt cgatcgccag ggtcgcgaca
     3841 ttgccgagca tcaccggccc gcgcgcgccg ggcacggcca ggcgcgcgag caggtcgagg
     3901 tcggtgcggg cgtcggcggc cagcttcacg acgatcggca cctggcgctg cgacaggttc
     3961 agtttcgcga gcgcctggtc gtagtcgccg gcggtcgcga cgcgcaaggt gtcggcgatc
     4021 gccgccgagg tgacgccgag gtcggcggcg cgcgccgaat cgggccgcac gatcagctcg
     4081 gggcgcacca ggctgctggt cgagacgacg ttgccgatgc cgcggatcgt gcgcagctca
     4141 cgctcgacct ggcgcgcgtg cgcgccgagc gcgtcgccgt cttcgcccgc cagcaccagc
     4201 atgtacttct cgctcgagcc gccgaagccg accttgatgc gcgcgccggg gatcaccgcc
     4261 agcgcctcgc gcaactgctc ctcgaccgcc tgcttcttga cgccgtggcg ctcgtcgcgc
     4321 ggcgtcatgt tgatcgtcag cgtcgccttg cgcacctcgg gcgcgcccgc cggcgcgaac
     4381 gggtcgccgc cggtcgcgcc gccgccgatc gcggtgtaga ccagcttcac gtccttgttc
     4441 tgctgcacga tctcgcgcgc cgcctcggca gccgcgaagg tctgcgcgaa ggtcgcgccc
     4501 ggcggcagcg cgatcgagac ctgggtctgc gacaggtcgt ccggcgggat gaagccggtc
     4561 ggaagcagcg gcaccagcat gaacgagccg accaggaaga cggtcgccgc gatcgtcgtc
     4621 aggatgcggt gcttcagcgc cgccttgacc cagcccatgt agatcgcgat ccagcggcct
     4681 tcgcggtgcg gcttcgtcgg ccgcttcagg atgtacgccg ccatcatcgg cgtcagcatg
     4741 cgcgcaacga ccagcgacat gaacaccgcg atcgccgccg tccagccgaa ctgcacgaaa
     4801 aacttgcccg gcacgccgct catgaacgcg gtcggcagga acaccgcgat caacgtgaag
     4861 gtggtcgcga tcaccgccag gccgatctcg tcggcggcct ccagcgcagc atcgaatggc
     4921 gacttcccca tgcgcagatg ccgcatgatg ttctcgatct cgacgatcgc gtcgtcgacc
     4981 aggatgccga cgacgagcga cagcgacagc agcgtcacgc cattcagcgt gaaaccgaac
     5041 aagtacatca cgccgaacgc cgggatgatc gacagcggca gcgcggtcgc cgagaccagg
     5101 gtcgcgcgcc agtcgcgcag gaacaggaac acgaccagca ccgcgagcgc cgcgccttcg
     5161 tacatcagca ccatcgagcc gtcgtagttc tcctgcaccg ggtcgacgaa gttgaaggcc
     5221 tcggtgatga cgatctcggg atgcgcctgt ttcagcgtct cgagctgcgc acgcacgccg
     5281 gcggcgacct cggtctcgcc tgcgccgcgc gtgcgcacga tctaga
//
LOCUS       DASRDG010000721         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_570658, whole genome shotgun sequence.
ACCESSION   DASRDG010000721 DASRDG010000000
VERSION     DASRDG010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570658"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..347
                     /gene="rpsN"
                     /locus_tag="VFA35_12080"
     CDS             42..347
                     /gene="rpsN"
                     /locus_tag="VFA35_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HZT56956.1"
                     /translation="MAKTNMIERDKRRSKAVRKYAALRTRLKETIRNPRSSPEERQAA
                     QAKLQALPRDASPTRQRNRCAITGRSRGVYSKFGLARTKIREVAARGEIPGLAKASW"
     gene            361..756
                     /gene="rpsH"
                     /locus_tag="VFA35_12085"
     CDS             361..756
                     /gene="rpsH"
                     /locus_tag="VFA35_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZT56957.1"
                     /translation="MSMTDPVADLLTRIRNGQSARKTQVSMASSKLKAAIVGVLKSEG
                     YIADFSVDAGAGKATLTVDLKYYEGRPVIDRIERVSRPGLRVYRGKEQLPRVQGGMGT
                     VIVSTPKGVMTDRAARAIGQGGEVLCIVA"
     gene            792..1322
                     /gene="rplF"
                     /locus_tag="VFA35_12090"
     CDS             792..1322
                     /gene="rplF"
                     /locus_tag="VFA35_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZT56958.1"
                     /translation="MSRVAKQVINIPAGVTVTVAGSEVTVKGAKGALTLPIAAGVAVA
                     QQDKQLQVSYAGEGAARTNAGSTRAHLANMVLGVTRGYDRKLELVGVGFRAAVAGKAL
                     NLTLGFSHPVSYPIPEGISIETPSPTEILVKGADRQRVGQTAAEIRGIRPPEPYKGKG
                     VRYADEVISLKEGKKK"
     gene            1333..1686
                     /gene="rplR"
                     /locus_tag="VFA35_12095"
     CDS             1333..1686
                     /gene="rplR"
                     /locus_tag="VFA35_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZT56959.1"
                     /translation="MSKKERRERRATKTRAKIRELGTPRLTVHRTPRHIYAQLFAADG
                     SRVLASASTVQKELVKELKYTGNVEAARAVGRAIAERAKAAGVGRASFDRSGFQYHGR
                     VKALAEAAREAGLEF"
     gene            1711..>2021
                     /gene="rpsE"
                     /locus_tag="VFA35_12100"
     CDS             1711..>2021
                     /gene="rpsE"
                     /locus_tag="VFA35_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZT56960.1"
                     /translation="MARMDKTQSADDMLEKLVAVNRTAKVVKGGRQFGFTALTVVGDG
                     AGRVGFGFGKAREVPVAISKAMAQARKNMVNVSLNNDTLHYAVRGAHGATRVLMQPAS
                     SG"
BASE COUNT          374 a          680 c          702 g          265 t
ORIGIN      
        1 gttccgcaag tgagccactg agctccaccc gaggatttgg catggccaag acgaacatga
       61 tcgaacgcga caagcgccgg agcaaggcgg tacgcaagta cgccgcgctg cgcacgcgcc
      121 tgaaagaaac gatccgcaac ccgaggtcct cgcccgagga gcgccaggcc gcgcaagcga
      181 agctgcaagc gctgccgcgc gatgccagcc cgacgcgcca gcgcaatcgc tgcgcgatca
      241 ccgggcgttc gcgcggtgtg tacagcaagt tcggtttggc gcgcaccaag atccgtgagg
      301 tcgcggcgcg cggcgagatt cccgggctgg ccaaggcgag ctggtaggag agcctagacg
      361 atgagcatga ccgatccggt tgccgacctg ctgacccgaa tccgcaacgg gcagagcgcg
      421 cgcaagaccc aggtttccat ggcttcctcg aagctcaagg ccgccatcgt tggcgtgctc
      481 aagagcgagg gctacatcgc cgacttcagc gtcgatgccg gcgccggcaa ggcgacgctg
      541 accgtcgacc tcaagtacta cgagggccgg ccggtgatcg atcgcatcga gcgcgtcagc
      601 cgcccggggc tgcgcgtcta tcgcggcaag gaacagctgc cgcgcgtgca gggcggcatg
      661 ggcacggtga tcgtgtcgac gcccaagggc gtgatgaccg accgcgcggc gcgcgccatc
      721 ggccagggcg gcgaggtgct ctgcatcgtc gcctaggcgc cgccgcagtc cttcttttag
      781 agacagagat tatgtccaga gtcgcaaaac aggtgatcaa cattcccgcc ggcgtcacgg
      841 tcacggtggc cggcagtgaa gtaacggtca agggcgccaa gggcgcgctg acactgccga
      901 tcgctgcggg cgtggcggtc gcgcagcagg acaagcagct gcaggtgtcc tacgccggcg
      961 agggcgcggc gcgcaccaac gccggttcga cgcgcgcgca cctcgccaac atggtgctgg
     1021 gcgtgacgcg cggctacgac cgcaagctcg agctggtcgg cgtcggtttc cgcgccgcgg
     1081 tggccggcaa ggcgctcaac ctgacgctcg ggttctcgca cccggtcagc tacccgatcc
     1141 cggagggcat cagcatcgag acgccgagcc cgaccgagat tctggtcaag ggtgccgacc
     1201 gtcagcgcgt cggccagacg gcggccgaaa tccgcggtat ccgcccgccc gagccctaca
     1261 agggcaaggg cgtgcgctac gcggacgaag tcatttccct caaggaaggg aagaagaagt
     1321 agtgccatga acatcagcaa gaaggagcgg cgcgagcgcc gcgccaccaa gacccgcgcc
     1381 aagatccgcg agctcggcac cccgaggctg accgtgcacc gcacgccgcg ccacatctac
     1441 gcgcagctgt tcgcggccga cggttcacgc gtgctggcct ccgcctcgac cgtccagaag
     1501 gagctggtca aggagctcaa gtacaccggc aacgtcgagg ccgcgcgcgc ggtcggccgc
     1561 gccatcgccg agcgggccaa ggcggccggc gtcgggcgcg cctcgttcga ccgctcgggt
     1621 ttccagtatc acggccgcgt caaggcgctg gccgaggccg cccgcgaagc cgggctcgaa
     1681 ttctagcgcc gcgccaggac ccaattactt atggcaagaa tggacaagac ccaaagcgcc
     1741 gacgacatgc tggagaagct ggtcgcggtc aaccgcacgg ccaaggtcgt caagggcggc
     1801 cgccagttcg gcttcaccgc gctgacggtg gtcggcgacg gcgccggccg cgtcggcttc
     1861 ggcttcggca aggcgcgcga ggtgccggtg gcgatctcca aggccatggc ccaggcgcgc
     1921 aagaacatgg tcaacgtcag cctgaacaac gacacgctgc actacgcggt gcgcggcgcc
     1981 catggtgcga cgcgtgtgct gatgcagccg gcttcgagcg g
//
LOCUS       DASRDG010000722         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_920483, whole genome shotgun sequence.
ACCESSION   DASRDG010000722 DASRDG010000000
VERSION     DASRDG010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920483"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="VFA35_12105"
     CDS             <1..1899
                     /locus_tag="VFA35_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56961.1"
                     /translation="VTTELASLRKQPNATALADTLAAADAALRRADADAQAAAALDDP
                     LPGTEAARADVKRVREDLAAAKAAAAQLGPAVALQAAAQRAATVPAIRAEVAKLRRAF
                     AAAAKGPGAELAKLPLEVGEAALALAESELTAKKTPAAAQALQGAAQQLLRVKMLQAD
                     AQRQDQARKALRARLAALRARPDAKAIETRLAAVDAALAQSESPKDEAEAAAALHAAE
                     AALQQADRDATARSAFDTRVDGLEREARQLTDGLGVDFEKAIAAARRHADALRFDAAT
                     KSLDKAEMLWERVRIGLLAQLRPADPQVLAGVKRLVQLGDAESVDAVVKHLPDNTPDA
                     VIAQIAQARFGVPFELHGPQATAGAKHVYAMLAKAPQDVKGNPSLAKVTYSTTPALAN
                     VSARGGQYDSEHEDAQTWGRPDVEQQAFGTAQKAPGSKKPQLPDNVEERCRPLNEKPV
                     EILDWAALHELGHSLDDATGFMKRRGKDDDCGGWIEYGADVQPIANAVARWAGVGNEP
                     AFVQHLLAWLQGGTPPPPPRPARVDADTWKGALVKVEGWLKRSTTGQIWYDNAASMAH
                     RIDDRIYQRPYPDEWVSYKAEARQRGLTGFQFKAPGEWFAELYAGYRSGKLKPNHPAM
                     DWLKDLHI"
BASE COUNT          425 a          820 c          868 g          291 t
ORIGIN      
        1 gtcaccaccg agctcgcctc gttgcgcaag cagcccaacg ccacggcgct tgccgacacg
       61 ttggcggcgg cggatgcggc cctacggcgc gccgacgcag acgcgcaggc tgccgcggcg
      121 ctcgacgatc cactgcccgg caccgaggcc gcccgcgccg acgtcaagcg tgtgcgcgag
      181 gatctcgccg cagcgaaagc cgcagccgcg cagctcggcc cggccgtcgc actgcaggcc
      241 gcggcgcagc gggcggccac ggttccagcg atccgcgccg aggtcgcaaa gctgcgtcgc
      301 gccttcgcgg cggcggcgaa ggggcctggc gcggagctgg ccaagctgcc gctcgaagtc
      361 ggcgaggccg cgcttgcgct cgcagaatcg gagctgacgg ccaagaagac gccggccgcg
      421 gcgcaggcgc tgcaaggcgc ggcccagcag ctgctgcgcg tgaaaatgct gcaggccgat
      481 gcgcagcgcc aagaccaggc gcgcaaggcg ctgcgcgcgc ggctcgccgc gttgcgcgcc
      541 cgaccggacg cgaaggcgat cgagacgcgg ctggcagcgg tcgatgccgc gctcgcgcaa
      601 tcggagtcgc cgaaggacga ggccgaggcc gccgcggcgc tgcacgcggc cgaggcggcg
      661 ctgcaacagg ccgaccgaga cgccacggcg cgctcggcgt tcgacacgcg ggtcgatgga
      721 ctcgaacgcg aagcccgtca gctgaccgat ggcctcgggg ttgatttcga gaaggcgatc
      781 gcggccgcac gccgccatgc cgacgcgctg cgcttcgatg cggcgacgaa gtcgctcgac
      841 aaggcggaga tgctgtggga gcgcgtgcgc atcgggctgc tcgcgcagtt gcggccggcc
      901 gatccgcagg tgctcgcggg cgtgaagcgg ctcgttcaac tgggcgacgc cgagagcgtc
      961 gatgcggtcg tcaagcacct gccggacaac acgcccgacg cggtcatcgc gcagatcgcc
     1021 caggcgcgct tcggcgtgcc gttcgaactg cacggaccgc aggcgaccgc cggggcgaag
     1081 cacgtctacg cgatgctcgc gaaggcaccg caggacgtga agggcaaccc gagcctcgcg
     1141 aaggtgacct acagcacgac gcctgcgctt gcgaacgtct ccgcccgtgg cgggcagtac
     1201 gacagcgagc acgaggacgc gcagacctgg gggcgtccgg acgtggagca gcaggccttc
     1261 ggcacggcac agaaggcgcc gggtagcaaa aagccccagc tgccggacaa cgtcgaggag
     1321 cgctgccgtc ctctgaacga gaagccggtg gaaattctcg actgggccgc gctgcacgag
     1381 ctcgggcact cgctcgacga cgcgaccggc ttcatgaagc ggcgcggcaa agacgacgac
     1441 tgcggcggat ggatcgagta cggcgcggac gtgcagccga tcgcgaacgc ggtcgcgcgg
     1501 tgggcgggtg tcggcaacga gccggcgttc gtgcagcacc tgctcgcgtg gctgcagggc
     1561 gggacacccc cgcctccgcc gcggccggct cgcgtcgacg ccgacacgtg gaagggcgcg
     1621 ctggtgaagg tcgaaggctg gctcaagcgc tcgaccaccg ggcagatctg gtacgacaac
     1681 gccgccagca tggcgcaccg cattgacgac cgcatctacc agcggcccta tcccgacgaa
     1741 tgggtcagct acaaggccga ggcgcgccag cgcggtctca ccggcttcca gttcaaggca
     1801 ccgggcgagt ggttcgcgga gctgtacgcg ggctaccgca gcggcaagct caagcccaac
     1861 catcccgcga tggactggct gaaggacctg cacatctagt ccgctgaatc agtgaagccg
     1921 aaggcgtctc caactcgcag gcgcatctcc aatctcaacg attcagcgga ctagcggcgc
     1981 ccgagaagga cttccgcttg ggcctgatgg aaaccttgaa actgacgccg ccggccgcgc
     2041 aggcgggcgg tgctgcgtcg tcgtccgatg ccaggctcga cgtagggctc catcagccgg
     2101 gtcccggtgg ccacgaacgg ctgcttcgta gacgcgaagc gcgcaagagg tcggtccgag
     2161 cgacaggcag ctttggacaa gccggacagg caggtttggg tcgagagtgc cagttgggcc
     2221 ctttgatcag cggacgttcg catgacccga acgagccgac aaaaggggaa cgtcggcagt
     2281 acgcctagag cggcccccca gcacaagcgc tcttcggtgc atggatgacc gcaatatggc
     2341 cgacagccgc cgctctggct gcagccacga ccgacagcag tcgctgcact gctgacgctc
     2401 agga
//
LOCUS       DASRDG010000723         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_688923, whole genome shotgun sequence.
ACCESSION   DASRDG010000723 DASRDG010000000
VERSION     DASRDG010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688923"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFA35_12110"
     CDS             <1..280
                     /locus_tag="VFA35_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56962.1"
                     /translation="RVGRPGEGYEGIRKGRVNLAGRVLLADDAGAFGAPTADSARTQV
                     SAATRAVLAVIYCPTERPSEHLTAMLARVADLLSRHCAATVHLVQTRQ"
     gene            436..987
                     /locus_tag="VFA35_12115"
     CDS             436..987
                     /locus_tag="VFA35_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZT56963.1"
                     /translation="MKQRLGWLLAVSFAALAVTVGVARAGSIGVGVFGGTGVPVVQAD
                     EGNGPVFGLRAPVKLVPLFTLEPFYSSQSLGDVTLDISPGVTATREGSDVKAYGLNAL
                     LTFGGPVTFYPFAGIGHAHYTRSGQDESSAAYQLGLGLGLSPLPKLTIDLRAELQAAV
                     DGSTSRKVGNFTLGASFALFHLP"
     gene            1002..1538
                     /locus_tag="VFA35_12120"
     CDS             1002..1538
                     /locus_tag="VFA35_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56964.1"
                     /translation="MRTLRNAVPLLALAAFAAAGCFLISGQFAVSYSLPSPLPAVGTA
                     TLVGVPVDLNTIGDYRSHKRYLKRVDDTALIGDFRNNSGSAANVEVWLVPSGALTLPA
                     NQLAANGTLLWGPLAIAAGATEHVDWNRSATLLVGRQALTDEIQGDGQFALYIVANGT
                     FDLTLTHGAVIAVISAAQ"
     gene            1598..2239
                     /gene="yihA"
                     /locus_tag="VFA35_12125"
     CDS             1598..2239
                     /gene="yihA"
                     /locus_tag="VFA35_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZT56965.1"
                     /translation="MRIDSAEFVLSAGEVSECPAGERPEIAMSGRSNVGKSSLLNALL
                     RRKALAKVSGTPGKTQRLGYILVNDRFHLVDLPGYGYAHAAGSARNRWAAMMQGYLRA
                     RRQLVAVLQLVDSRHEPSREDREMVRWLGEERLPFCLVATKIDKVGSSKREPAMQALL
                     RALELPDTQPRVACSSETGEGRDAVLAWIEQALESAARAGDSTRASRRGGDRG"
BASE COUNT          301 a          826 c          854 g          348 t
ORIGIN      
        1 gcgcgtcggc cgcccgggcg agggctacga gggcatccgc aagggccgcg tgaacctcgc
       61 cggtcgcgtg ctgctggccg acgacgccgg cgcgttcgga gcgcccaccg cggactccgc
      121 ccgcacgcag gtcagcgccg cgacgcgcgc ggtgctcgcg gtcatctact gcccgaccga
      181 gcggccgagc gagcacctca cggcgatgct ggcccgcgtc gccgacctgt tgtcgcgcca
      241 ctgcgcggcc accgtccacc ttgtgcagac gcgccagtaa cggttgcggc gcccacgcgc
      301 aacgcgcggt ttcgcaaccg ttcacggctg cacggcaggc ctgcgcttga ccctccccgt
      361 gcgctcggcc agcctttggg ctgcgttccc cgaccggccc gtggcgcggg ccgcagcacg
      421 cggaaggagt ggacgatgaa gcagcgtctg gggtggttgc tcgccgtgtc gttcgccgcg
      481 ctcgcggtga cggtgggcgt ggcgcgcgcc ggcagcatcg gcgtgggcgt gttcggcggc
      541 acgggcgtgc cggtggtgca ggcggacgag ggcaacggcc cggtgttcgg cctgcgcgcg
      601 ccggtcaagc tggtgccctt gttcacgttg gaaccgttct actcgtcgca gtcgctcggc
      661 gacgtgacgc tcgacatctc gccgggcgtg accgcgacgc gggagggctc cgacgtcaaa
      721 gcctacgggc tcaacgcgct gttgacgttc ggcggaccgg tgacgttcta tcccttcgcc
      781 ggaatcgggc acgcgcacta cacgcgctcg gggcaggacg agtcgtccgc ggcctaccag
      841 ctgggcctgg ggttgggcct gtcgccgctg ccgaagctca ccatcgacct gcgcgccgag
      901 ctgcaggccg cggtcgacgg cagcacgtcg cgcaaggtgg gcaacttcac gctgggcgcc
      961 agcttcgcgc tgttccacct gccgtgaaag gggccgcgac catgcgcacg cttcggaacg
     1021 cggtgccgct cctggcgttg gccgcgttcg cggcggccgg gtgcttcctc atctcgggcc
     1081 agttcgccgt cagctattcg ctgccgagcc cgctgccggc ggtgggcacg gccacgctcg
     1141 tcggcgtgcc ggtcgacctc aacacgatcg gcgactaccg cagccacaag cgctacctca
     1201 agcgcgtcga cgacacggcg ctgatcggcg acttccggaa caacagcggt tcggccgcca
     1261 acgtcgaggt gtggctggtg ccttcgggag cgctgaccct gccggccaat cagctggcgg
     1321 cgaacgggac cttgctgtgg ggcccgctcg cgatcgctgc cggtgccacc gaacacgtcg
     1381 actggaatcg ctcggccacg ctgctggtgg gccggcaagc gctgaccgat gagatccagg
     1441 gcgatgggca gttcgccctc tacatcgtcg ccaacggcac gttcgacctc accctcaccc
     1501 acggcgccgt gatcgccgtc atcagcgcgg cgcagtaggg gcgcgcgacc ggggttcgcc
     1561 ccgccgccgt tcatccgccg cacggaggtc cgacggcatg aggatcgatt ccgccgagtt
     1621 cgtgctgagc gcgggagagg tgtcggagtg ccccgccggc gagcggccgg agatcgccat
     1681 gtccgggcgc tcgaacgtcg ggaagtcgtc gctcctgaac gcgctgctgc ggcggaaggc
     1741 gctggcgaag gtctccggca cgcccggcaa gacgcagcgg ctgggctaca tcctcgtcaa
     1801 cgatcgcttc cacctcgtgg accttccggg ctacggctac gcgcacgccg caggctccgc
     1861 acgcaaccgc tgggccgcga tgatgcaggg ctatctccgt gcgcgccgcc agctcgtggc
     1921 ggtgttacag ctggtcgact cgcgccacga gccgtcgcgc gaggatcgcg agatggtgcg
     1981 ctggctgggc gaggagcggc tgccgttctg cctggtggcg accaagatcg acaaggtcgg
     2041 ctcgagcaag cgcgagccgg cgatgcaggc gctgttgcgc gcgctggagc tgccggacac
     2101 gcagccgcgc gtcgcctgct cgagcgagac gggcgagggc cgggacgcgg tgctggcgtg
     2161 gatcgagcag gcgctcgagt cggcggcccg ggccggcgac tcgacccgcg cttcacgccg
     2221 cggcggcgat cgcggctgag cgcgaccgcc cgcgtctcgc gtggggcctg ttaactcgct
     2281 ggcgttcgag cgcgtgcttt cggcccgccg ggtcggctag cgccactac
//
LOCUS       DASRDG010000724         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1722285, whole genome shotgun sequence.
ACCESSION   DASRDG010000724 DASRDG010000000
VERSION     DASRDG010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722285"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2085)
                     /locus_tag="VFA35_12130"
     CDS             complement(<1..>2085)
                     /locus_tag="VFA35_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZT56966.1"
                     /translation="DGRLHVQVQPGDAVLTLNARATAPFTDVAAHLPAAPWPKQEIWS
                     YEAAPRLRVTNAVAAVSVDPQQAQVPAQWQGLPAFALGDGEKIAIEERSRGLAPGEAN
                     RLSLQREAWLDFAGTGWYAKDVVSGSMVQGWRLDVAAPFKLERAQASAGGGEALLVTR
                     GANEAWSGVEWRTPNVHLAGSVRIDAVSNLAATGWQQGFDRIDTTLHFPYGYKLLGAP
                     GADSVHGSWMSRWTLLDVFVAAIVALLAWRLLGLFGALAAVVYLGLGYQESGSPLWTL
                     VAVLALALIVRALPAGKLQTAALWLRRAAMLALVLVALPFAANQVRYALYPQLEEGQG
                     AWTYHAARPHGPYLNDDGLARDEMEAGAAQNMATPAPAAAPPPPPPEPMAAAGGKLHR
                     TLSAPKIAEKAKSADQDKKPDSVTVTGSRVLQSQLIDHYSQSTVMQTGAGEPGWSIGS
                     TAQLGWGGPVTPAQNVRLVIAPPWLVRPLRIVLALLLAWLAWSGWRGRPATPAPSRAL
                     ATGLATLLLASGLAVAPLAQAQDVPSDELLSQLRERLTEAPKCAPACASIAQMQVAAT
                     GDALGIVLEVHAGERLAVPLPRADASITLKSVKVDNVADVAVARAADGGDWIALDRGV
                     HRVEAAYVAHADRLALNFVLVPARVLFEGKGWSANGIEDEHLLGGSLSLARARDDAGA
                     KPELGAQQFPTYV"
BASE COUNT          302 a          761 c          737 g          285 t
ORIGIN      
        1 gacgtaggtg gggaactgct gcgcgccgag ttcgggcttc gcgcccgcat catcgcgcgc
       61 gcgtgcgagg ctcagcgaac cgccgagcag gtgctcgtcc tcgatgccgt tcgcgctcca
      121 gcccttgcct tcgaacagca cgcgcgcggg cacgaggacg aagttcagcg cgaggcggtc
      181 ggcgtgcgcg acataggccg cctcgacacg atgcacgccg cggtcgagcg cgatccagtc
      241 gccgccgtcg gcggcgcgcg cgaccgccac gtcggcgaca ttgtccactt ttacactttt
      301 cagcgtgatg ctcgcgtccg cacgcggcaa cggcacggcg aggcgctcgc ccgcgtgcac
      361 ttcgagcacg atgccgagcg cgtcgccggt cgcggcgacc tgcatctgcg cgatgctcgc
      421 gcaggccggc gcgcacttcg gcgcctcggt caggcgttcg cgcagctggc tcagcagctc
      481 gtcggacgga acatcctgcg cctgcgcaag cggcgcgacg gcgaggccgc tcgcgagcag
      541 cagggtcgca aggccggtgg ccagcgcgcg cgacggcgcc ggcgttgccg ggcgcccgcg
      601 ccagccgctc cacgcgagcc aggccagcag cagcgcgagc acgatgcgca gcggccgcac
      661 gagccacggc ggcgcgatga cgaggcgcac gttctgcgcc ggcgtgaccg ggccgcccca
      721 gccgagttgc gcggtgctgc cgatgctcca gcccggctcg cccgcgccgg tctgcatcac
      781 ggtggactgg ctgtagtgat cgatcagttg ggattgcaaa accctgctgc cggtgacggt
      841 gacggaatcg ggcttcttgt cctggtccgc ggatttggct ttctcggcga tcttcggcgc
      901 ggacagggtc cgatgaagct tgccgcccgc agcggccatc ggttccggtg gcggcggagg
      961 cggcgctgca gcaggcgcgg gcgttgccat gttctgcgcg gcgcccgctt ccatttcatc
     1021 gcgcgccagc ccgtcgtcgt tgagatacgg accgtgcggg cgcgcggcat ggtacgtcca
     1081 cgctccctgc ccttcctcca gttgcggata cagcgcgtag cgcacctggt tcgccgcgaa
     1141 cggcagcgcg acgaggacca gggccagcat cgccgcgcgg cgcagccaca gcgcggcggt
     1201 ttgcagcttg cccgcaggca gcgcgcgcac gatcaaggcg agtgcgagca cggcgaccag
     1261 cgtccacaac ggcgaaccgg attcctggta gccgagcccg agatagacaa ctgcggccag
     1321 cgcgccgaac aggccgagca gacgccaggc gagcagcgcg acgatcgcgg cgacgaagac
     1381 gtcaagcagg gtccagcgcg acatccaact gccgtgcacg ctgtccgcac cgggcgcgcc
     1441 gagcagcttg tagccgtagg gaaaatgcag ggtcgtgtcg atgcggtcga agccctgctg
     1501 ccagccggtc gccgccaggt tcgacaccgc atcgatgcgc accgagcctg cgagatgcac
     1561 gttcggcgtg cgccactcga cgccggacca ggcctcgttc gcgccgcgcg tgaccagcag
     1621 cgcctcgccc ccgcccgcac tcgcctgcgc gcgctcgagc ttgaacggcg cggcgacgtc
     1681 gaggcgccag ccctgcacca tcgaaccgga cacgacgtcc ttcgcatacc agcccgtgcc
     1741 ggcgaaatcg agccaggcct cgcgctgcag gctcaggcga ttggcctcgc cgggcgcgag
     1801 gccgcgcgag cgttcctcga tcgcgatctt ctcgccgtcg ccgagcgcga acgcgggcag
     1861 gccctgccat tgcgccggca cctgcgcctg ctgcggatcg accgagaccg ccgccacggc
     1921 attggtcacg cgcaggcgcg gcgcggcttc gtaactccag atttcctgct tcggccacgg
     1981 cgccgcaggc aggtgcgcgg ccacgtccgt gaacggcgcg gtcgcgcgcg cgttcaaggt
     2041 cagcaccgca tcgcccggct gcacctggac gtgcaggcgg ccgtc
//
LOCUS       DASRDG010000725         5633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1723907, whole genome shotgun sequence.
ACCESSION   DASRDG010000725 DASRDG010000000
VERSION     DASRDG010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723907"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /gene="dxs"
                     /locus_tag="VFA35_12135"
     CDS             <1..1117
                     /gene="dxs"
                     /locus_tag="VFA35_12135"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZT56967.1"
                     /translation="LKTLKDLKGPQLLHIITTKGKGYAPAEREQIEYHAVGPFDPVKG
                     LAKKGPGKITYTEVFGNWLCEMAAEDSRLYGITPAMREGSGLVRFSKEFPQRYFDVGI
                     AEQHAVTFAAGLACEGLKPVVAIYSTFLQRAYDQLIHDVAIQNLPVVFALDRAGLVGA
                     DGATHAGNYDIAYLRCIPNMSVLTPADERECRAALTTAFRQDHPVSVRYPRGAGAGVA
                     AGTDLPELPFGKGEMRRTIARAAAQGGPRVAILAFGTLLYPALAAAERVDATVANMRF
                     VKPLDVELVTELAGSHDAIVTVEDGCVAGGAGSAVQEALASAGIQIPVLALGLPDAFI
                     EHGDPAQLMAMCGLDAAGIEQAILKRFGARPALVRSA"
     gene            1226..2017
                     /gene="folE2"
                     /locus_tag="VFA35_12140"
     CDS             1226..2017
                     /gene="folE2"
                     /locus_tag="VFA35_12140"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HZT56968.1"
                     /translation="MSNLKDALHIPDTQGARDERHLAIQRVGIKGVRYPLELRIGNTV
                     APTVALWSLDVALPAEQKGTHMSRFVAWLDALDAPLDARTLRTGLAGMLDKLHATEGR
                     IDAQFPFFLRKRAPVSGVQSLLEYQGRWLAESVGGTQTIWAEVVVPVKSLCPCSKEIS
                     DYGAHNQRSHVTIRVELLEDIGWPELVRFAEDSASSEIWPMLKRADEKWVTERAYENP
                     KFVEDMVRDVALRLNADARVGRYRVDVENFESIHAHSAFARIERA"
     gene            complement(2050..2703)
                     /locus_tag="VFA35_12145"
     CDS             complement(2050..2703)
                     /locus_tag="VFA35_12145"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZT56969.1"
                     /translation="MSQYFEVHPENPQGRLLKQAAQILHGGGIAAIPTDSSYALVCHL
                     DDKAAAENLRRLRGFDDRNGDKHHLTLLCRDLSELASYARVDNRQYRLLKLGTPGPFT
                     FILEATKEVPRRLSHPSRRTIGLRVPAHAVTQALLEVLGQPLLATTLIVPGETEPMNE
                     PHAIRDRFEKRVQAIVDAGACPMQPTTVVDLTGATPVLIRQGRGDPALLGLSVEEEA"
     gene            complement(2732..3601)
                     /locus_tag="VFA35_12150"
     CDS             complement(2732..3601)
                     /locus_tag="VFA35_12150"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HZT56970.1"
                     /translation="MNAPHPLNADLHCHSAVSDGTLEPEAVAARAKANGVELWALTDH
                     DEIGGQQRALAAAAALGLPCLTGTEISVTFIDTTVHIVGLGFDADDAALAAGLRATRG
                     GRRDRALEMSDELAKVGIRGAYDGALQYVGNPELISRTHFARFLVESKVCADTYEVFR
                     KYLVEGKPGFVPHRWATLKNAVTWITEAGGIAVIAHPARYKLTANEEYALFSEFKAHG
                     GRGVEVVTGSHSTADAARYADAAIEYELLASRGSDFHSPGESHTDLGALPALPERLTP
                     VWAALEARVHRPA"
     gene            complement(3646..4773)
                     /locus_tag="VFA35_12155"
     CDS             complement(3646..4773)
                     /locus_tag="VFA35_12155"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZT56971.1"
                     /translation="MNRRRLALIATLVGVVLMLSPGLSAAQALKPVTVARGLVHPWGL
                     AFLPDGRMLVTERPGRMRVVAPDGRLGEPLQGLSKVEVGGQGGLLDVAIDPGFAANGL
                     VYWSFSEADPQGGRGNSTAVARGRLDGNRLVDVRLIFRQMPKFESAAHFGSRLVFARD
                     GRLFVTLGDRFRRRDDAQSLDNHHGKIVRIESDGRVPADNPFVGPNKVPGALPEIWSY
                     GHRNVQGAALHPVTGELWADEHGPQGGDELNLVRAGKNYGWPVITYGAEYVTGAKIGE
                     GTAKAGMEQPLTYWVPSIATSGLAFLTSDRYPGWKGNAFIGALKAKLLVRLELDGAKV
                     VREERLLQDLGERIRDVRQGPDGWLYLLTDSPEGRILRLER"
     gene            complement(4836..>5633)
                     /locus_tag="VFA35_12160"
     CDS             complement(4836..>5633)
                     /locus_tag="VFA35_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZT56972.1"
                     /translation="DAARATGLARTLDAINRERREVETGMREQAEALLAQLAPLQDGD
                     APPPAALALFHPDFHEGVVGIVASRLKDRIHRPTFVFARGQDGLLKGSGRSIPGFHLR
                     DALDLLSKRHPEVLVRFGGHAMAAGCTLAEAHFDTFDAAFQQVAAEWLDAATLSRTLL
                     SDGPLGAEHFNAQTVRALDAQVWGQAFEAPVFVDEVEVVSQRLVGEKHLKLALRFAGE
                     ARGSALRDAIWFGHSEPVAAKLRLAYRLSVDEYNGRERVQMIVEAIG"
BASE COUNT          812 a         1963 c         2010 g          848 t
ORIGIN      
        1 gctgaagaca ctcaaggatc tcaagggccc gcagctgctg cacatcatca cgaccaaggg
       61 caagggctac gcaccggccg agcgcgagca gatcgaatat cacgccgtcg gtccgttcga
      121 tccggtcaag ggtctggcga agaagggtcc gggcaagatc acctacaccg aagtgttcgg
      181 caactggctg tgcgagatgg ctgcggaaga ctcgcgcctg tacggcatca cgccggcgat
      241 gcgcgaaggt tccggactgg tccgcttctc gaaggaattt ccgcaaagat acttcgacgt
      301 cggcatcgcc gagcagcacg ccgtcacctt cgccgcgggc ctggcctgcg aggggctgaa
      361 gccggtcgtc gcgatctact cgaccttcct gcagcgcgcc tacgaccagc tgatccacga
      421 cgtggcgatc cagaacctgc cggtggtgtt cgcgctcgac cgcgccgggc tggtcggcgc
      481 cgacggcgcg acccacgccg gcaactacga catcgcgtac ctgcgctgca tccccaacat
      541 gagcgtgctg acgccggccg acgagcgcga gtgccgcgcc gcgctgacga ccgcgttccg
      601 gcaggatcac ccggtgtcgg tgcgctaccc gcgcggcgcc ggcgccggcg tggccgcggg
      661 caccgatctg cccgagctgc cgttcggcaa gggcgagatg cggcgcacca ttgcgcgcgc
      721 ggcggcgcag ggcgggccgc gcgtcgcgat cctcgcgttc ggcacgctgc tgtacccggc
      781 actggccgcg gccgagcgcg tcgatgcgac ggttgcgaac atgcgtttcg tcaagccgct
      841 cgatgtcgaa ctcgtcaccg aactggccgg cagccacgac gcgatcgtca ccgtcgaaga
      901 tggctgcgtg gcgggcggcg ccggcagcgc ggtgcaagag gcgctggcga gtgccggcat
      961 ccagatcccg gtgctcgcgc tcggcctgcc cgacgccttc atcgagcacg gcgacccggc
     1021 tcagctgatg gcgatgtgcg ggctcgacgc ggccggcatc gagcaggcca tcctgaagcg
     1081 cttcggcgcg cggcccgcgc tggtgcgttc cgcctgaccc gttgaacctg gcgttgaacc
     1141 cagccgaacg gcggcttcaa atccgcgccg gggcgtgcac cggcatttgc tggcatgctg
     1201 cgcgtctggt gacaagatga aatccatgag caacctgaaa gacgcgctcc acatccccga
     1261 cacgcaaggc gcgcgtgacg agcggcacct ggcgatccag cgcgtcggca tcaagggcgt
     1321 gcgctacccg ctggagctgc gcatcggcaa cacggtcgcg ccgacggtcg cgctgtggag
     1381 cctcgacgtg gcgctgccgg ccgaacagaa gggcacgcac atgtcgcgct tcgtcgcctg
     1441 gctcgacgcg ctcgacgcgc cgctcgacgc gcgcaccttg cgcaccgggc tcgccggcat
     1501 gctcgacaag ctgcacgcga ccgagggccg catcgacgcg cagttcccgt tcttcctgcg
     1561 caaacgcgcg ccagtgagcg gcgtgcagag cctgctcgag taccaagggc gctggctggc
     1621 ggaatcggtg ggcggcacgc aaaccatctg ggccgaggtc gtcgtgcccg tgaagtcgct
     1681 gtgcccgtgc tcgaaggaga tctccgacta cggtgcgcac aaccagcgct cgcacgtgac
     1741 gatccgcgtc gagttgctcg aagacatcgg ctggcccgaa ctcgtgcgct ttgccgagga
     1801 ctcggcgtcg agcgagatct ggccgatgct caagcgcgcc gacgagaagt gggtcaccga
     1861 acgcgcctac gagaacccga agttcgtcga agacatggtg cgcgacgtgg cactgcggct
     1921 caatgccgac gcgcgcgtcg ggcgctaccg cgtcgatgtc gagaacttcg agtcgatcca
     1981 tgcgcacagc gcgtttgcgc gcatcgagcg cgcctgaaga ttccttcgcg tagcgagcgc
     2041 tgatcgccgt caggcctctt cctcgacgct gagccccagc agcgccgggt cgccgcggcc
     2101 ctggcggatc agcaccggcg tggcgccggt caggtcgacc acggtggtcg gctgcatcgg
     2161 gcaggcgccg gcgtcgacga tcgcctgcac ccgtttctcg aagcggtcgc ggatcgcgtg
     2221 cggctcgttc atcggctcgg tctcgcccgg cacgatcagc gtggtcgcga gcagcggctg
     2281 gccgaggact tcgagcaagg cctgcgtgac cgcatgcgcc ggcacgcgca gcccgatcgt
     2341 gcggcgcgac gggtgcgaga ggcggcgcgg cacttccttg gtcgcttcga ggatgaaggt
     2401 gaacggcccc ggcgtgccga gcttcagcag ccggtactgc ctgttgtcga cgcgcgcgta
     2461 gctcgcgagc tcgctcaggt cgcggcacag cagcgtgagg tggtgcttgt cgccgttcct
     2521 gtcatcgaaa ccgcgcaggc gccgcaggtt ctcggccgcg gccttgtcgt cgaggtggca
     2581 cacgagcgcg tagctcgaat cggtcgggat cgccgcgata ccgccgccgt gcaggatctg
     2641 ggcggcctgc ttcagcaggc gcccttgcgg gttctcgggg tggacttcga agtactggga
     2701 catgcgtgca ttgtcccgcg tgccgccgcg gtcacgccgg gcggtggacg cgtgcctcca
     2761 gcgcggccca gaccggcgtc agccgctccg gcagcgcggg cagggcgccg aggtcggtgt
     2821 ggctttcgcc ggggctgtgg aagtcgctgc cgcgcgaggc gagcagttcg tactcgatcg
     2881 ccgcgtcggc gtagcgcgcc gcatcggccg tgctgtggct cccggtcacg acctcgacgc
     2941 cgcgcccgcc gtgcgccttg aactcgctga acagtgcgta ctcctcgttc gcggtcagct
     3001 tgtagcgcgc cgggtgcgcg atcaccgcga tgccgccggc ttcggtgatc caggtgaccg
     3061 cgttcttcag cgtcgcccag cggtgcggca cgaacccggg tttgccttcg accaggtact
     3121 tgcggaacac ctcgtaggtg tcggcgcaga ccttgctctc gaccaggaag cgcgcgaagt
     3181 gcgtgcgcga gatcagctcg gggttgccga cgtattgcaa cgcgccgtca taggcgccgc
     3241 ggatgccgac cttcgcgagt tcgtccgaca tctccagcgc gcggtcgcgg cgcccgccgc
     3301 gcgtcgcgcg caggccggcg gcgagcgccg cgtcgtcggc atcgaagccg aggccgacga
     3361 tgtgcacggt cgtgtcgatg aaggtcaccg agatctcggt gccggtcagg caaggcaggc
     3421 cgagcgcagc ggccgcggcc agcgcgcgct gctggccgcc gatctcgtcg tgatcggtca
     3481 gcgcccacaa ctcgacgccg ttcgccttcg cccgcgccgc cacggcctcg ggttcgaggg
     3541 tgccgtcgga cacggcggag tggcagtgca ggtcggcgtt cagcgggtgc ggggcgttca
     3601 cgcggtcatt ctaggaggca ggcggacgca ccgcgtcgca gggggtcatc gctcgagccg
     3661 caggatgcgg ccctccgggc tgtcggtcag caggtagagc cagccgtcgg ggccctggcg
     3721 cacgtcgcgg atgcgctcgc ccaggtcttg cagcagccgt tcctcgcgca cgaccttggc
     3781 gccatcgagc tcgaggcgca cgagcagttt tgccttgagc gcgccgatga aggcgttgcc
     3841 tttccagccc gggtagcgat cgctggtcag gaacgcgaga ccgctggtcg cgatcgacgg
     3901 cacccagtag gtcagcggct gttccatgcc ggccttcgcc gtgccttccc cgatcttcgc
     3961 gccggtgacg tactcggcgc cgtaggtgat caccggccag ccgtagttct tgccggcgcg
     4021 cacgaggttc agttcgtcgc cgccctgcgg gccgtgctcg tcggcccaga gctcgccggt
     4081 cacggggtgg agcgcggcgc cttgcacgtt gcggtggccg tagctccaga tctcgggcaa
     4141 cgcgcccggt accttgttgg ggccgacgaa ggggttgtcg gccggcacgc ggccgtcgga
     4201 ttcgatgcgc acgatcttgc cgtggtggtt gtccagcgac tgcgcgtcgt cgcgccgccg
     4261 aaagcggtcg ccgagcgtga cgaacaggcg gccgtcgcgc gcgaacacga ggcgcgagcc
     4321 gaagtgcgct gcgctctcga acttcggcat ctggcgaaag atcaagcgca cgtcgaccag
     4381 ccggttgccg tcgagccggc cgcgcgcaac cgcggtgctg ttgccgcgcc cgccctgagg
     4441 atcggcctcg ctgaagctcc agtagaccag gccgttggcc gcgaagcccg ggtcgatcgc
     4501 gacatcgagc agcccgccct ggccgccgac ctcgaccttg gacaggcctt gcagcggctc
     4561 gccgaggcgg ccgtcgggcg cgaccacgcg catccgcccc gggcgctcgg tcacgagcat
     4621 gcgcccgtcg ggcaggaacg ccaggcccca cgggtggacg aggccgcgcg ccaccgtgac
     4681 cggcttcagc gcctgcgctg ccgacaggcc cggggagagc atcagcacga cgcccaccaa
     4741 agtggcgatg agggcgagac ggcgtcggtt catcggccca tgctgccgcg atcgccacgg
     4801 cctggcaagc gatgcctgca tgccggtgcc gcccgtcagc cgatggcctc gacgatcatc
     4861 tgcacgcgct cgcggccgtt gtattcgtcg acgctcaggc gataggcaag ccgcagcttc
     4921 gccgcgaccg gctcgctgtg gccgaaccag atcgcgtcgc gcagcgcgct gccgcgcgcc
     4981 tcgccggcaa accgaagcgc cagtttcaga tgcttctcgc cgaccaggcg ctgcgagacg
     5041 acctcgacct cgtcgacgaa caccggcgcc tcgaaggcct gcccccagac ctgcgcgtcg
     5101 agcgcgcgca ccgtctgcgc attgaagtgc tcggcgccga gcgggccgtc gctgagcagc
     5161 gtgcgcgaca gggtcgcggc atcgagccac tcggccgcga cctgctggaa cgcggcgtcg
     5221 aaggtgtcga agtgcgcctc ggcgagcgtg cagccggccg ccatcgcgtg gccaccgaag
     5281 cgcaccagca cctcggggtg gcgcttgctg agcaggtcga gcgcgtcgcg caggtgaaag
     5341 cccgggatcg agcggcccga gcctttcagc agcccgtcct gcccgcgcgc gaacacgaag
     5401 gtcgggcggt ggatgcggtc tttcaggcgc gacgcgacga tgccgaccac gccttcgtgg
     5461 aagtcggggt ggaacagcgc cagcgccgct ggcggcggcg catcgccgtc ctgcagcggc
     5521 gcgagctgcg cgagcagcgc ttcggcctgc tcgcgcatgc cggtctcgac ctcgcgccgt
     5581 tcgcggttga tcgcgtcgag cgtgcgcgcg agcccggtgg cgcgcgcggc gtc
//
LOCUS       DASRDG010000726         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_1380504, whole genome shotgun sequence.
ACCESSION   DASRDG010000726 DASRDG010000000
VERSION     DASRDG010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380504"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFA35_12165"
     CDS             <1..535
                     /locus_tag="VFA35_12165"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZT56973.1"
                     /translation="KQLQLLHDTVPGAKRIAVLINPDNPLHKSLMVQAAATAQQLSLE
                     LRAFEVRAPEAAGAAIEAARRERCQAVYSPGDAVLNSPALGLGQLATDAGLPFLSGLR
                     RQTEAGGLMSYGPDFLDLYRRAAILADRLFKGERAADIPIEQPSKYELVLNLKAAKAL
                     GLTIPRGVLLQADVAIE"
     gene            complement(630..1310)
                     /locus_tag="VFA35_12170"
     CDS             complement(630..1310)
                     /locus_tag="VFA35_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZT56974.1"
                     /translation="MKDGPNIARIAGLIGEPARAEMLTALLGGRALTATELAAAAGVT
                     KQTTSAHLSKLLEAKLLAVQAQGRHRYFRLADDEVAHALETLAGVAFRSRPTRLVTGP
                     REPALRKARVCYDHLAGELSVMVYHGLLASGSLASGTAGLCLSEAGLARFRDLGVDVD
                     AVAQQRRVFCRPCLDWGERRDHLAGALGAALLQRIYELRWAARVKDSRVVEFTAVGEQ
                     NLRSTFAT"
     gene            complement(1376..2170)
                     /locus_tag="VFA35_12175"
     CDS             complement(1376..2170)
                     /locus_tag="VFA35_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZT56975.1"
                     /translation="MNPPPIRFDDGAAYETYMGIWSQSVGDAFLRWLAPAPGWRWLDV
                     GCGNAAFTEMLVERCAPAEVQGIDPAEAQIAFARTRLPAGAPAAFQVGDAQALPWADA
                     HFDAAVMALVIFFVPDPAKAVAEMVRVTRPGGSVSAYAWDVLGGGLPYAAMMEEIKAM
                     GIALPAPPSEDASRLETMRSLWQDAGLVNVETRTIEVQRSFESFERFWEIAQTGPRVA
                     ARVAQMGEDERAHLRDGLRRRLPSVDAQGRLTYRAWANAIKGRVPG"
     gene            2303..3403
                     /gene="ada"
                     /locus_tag="VFA35_12180"
     CDS             2303..3403
                     /gene="ada"
                     /locus_tag="VFA35_12180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HZT56976.1"
                     /translation="MPTSKQEIMKSPSSDITRDPRWTSIVTRDKAADGSFWYSVATTG
                     VFCRPSCPSRLANPKNVRLHESPGAAEAAGFRPCRRCNPDGISIDVANAAVVTKACRL
                     IDEAAEPLSLTQLAEFVELSPHYFHRLFKKAMGLTPKAYAAAARSARLRRGLATAATV
                     TEAFHEAGFGSNGRFYEASAGVLGMSPTHYRSGGVQEVLTFAVGQCTLGAILVASSAK
                     GVAAILLGDDPEALLRDLQDRFPKARLVGGDAAYERLVAEVVGRVEEPARGFDLPLDV
                     RGTAFQQRVWQALRAIPPGATETYSSIALKIGAARSVRAVAGACAANLHAVAIPCHRV
                     VRQDGDLSGYRWGVERKRELIERESRAQERAA"
     gene            3418..>4081
                     /locus_tag="VFA35_12185"
     CDS             3418..>4081
                     /locus_tag="VFA35_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZT56977.1"
                     /translation="MEADVAARVQRIDTAALESDLEAQGWAVLHDLLDATECDDIAAL
                     YANGAGFRSQVVMARHGFGQGEYRYFSYPLPPLVHGLRTALYPLLAPLANRWHERMGM
                     DARFPERHDEFLAHCAASGQSRPTPLLLQYGAGDYNCLHQDLYGEHVFPLQVAVLLSE
                     PAKDFSGGEFVLTEQRPRMQTRAAVVPLTQGDAVVFAVNSRPVRGTRGDYRVKMRHGV
                     SKI"
BASE COUNT          613 a         1440 c         1423 g          605 t
ORIGIN      
        1 caagcagctg cagctgttgc acgacacagt gccgggcgcc aagcgcattg ccgttctcat
       61 caaccctgac aatcccctgc acaagagcct gatggtgcag gcagccgcta cggcgcagca
      121 actgagcctc gagttgcgag cgttcgaggt gcgcgccccc gaggcggccg gcgcggccat
      181 cgaagctgca cggcgcgagc gctgccaggc ggtgtactcg cccggcgacg ctgtgctcaa
      241 ctcgccggcg ctgggcctcg ggcagttggc cacggacgcc gggctgccct tcctgagcgg
      301 tttgcgcagg cagaccgagg cgggagggct gatgtcctac gggcccgact tcctcgacct
      361 gtaccgccgc gccgcgatcc tcgccgaccg cttgttcaag ggcgagcgcg ccgccgatat
      421 cccgatcgag cagccgagca agtacgaact ggttctcaac ctgaaagccg ccaaggcatt
      481 gggtctgacg attccgcgag gcgtgctgct gcaggccgac gtcgcgatcg aatgactctg
      541 tcgggttggt cttggccggc gggacacggg cccgcaggaa cgacggtccg gaaaactccg
      601 gcctgcgcca agcgcgtttc agcgctgcat caggtggcga aggtcgaccg caggttctgc
      661 tcgcccaccg ccgtgaactc caccacccgc gagtccttca cccgcgcggc ccagcgcagc
      721 tcgtagatgc gctgcagcag tgccgcgccg agcgcgccgg cgaggtggtc gcggcgctcg
      781 ccccagtcga ggcagggtct gcagaacacg cgccgctgct gcgcaaccgc gtcgacgtcg
      841 acgccgaggt cgcgaaagcg cgccaggccg gcttcgctca ggcagagacc cgcggtgccg
      901 ctggccagcg agccgctcgc cagcagcccg tggtagacca tcacgctcag ctcgccggcg
      961 agatggtcgt agcagacccg cgccttgcgc agcgccggct cgcgcgggcc ggtgaccagc
     1021 cgcgtcggtc ggctgcggaa ggccacgccg gccagcgtct cgagggcatg cgcgacctcg
     1081 tcgtcggcga ggcggaagta gcggtgccgg ccttgcgcct gcaccgccag cagcttggcc
     1141 tcgagcagct tgctcaggtg cgcgctcgtg gtctgcttgg tgacgccggc cgccgcggcc
     1201 agctccgtgg cggtgagtgc gcggccgccg agcagcgcgg tgagcatctc ggcgcgcgcc
     1261 ggctcgccga tgagaccggc gatgcgggcg atgttggggc cgtccttcat cgcccgcagt
     1321 ctaggcgcgg cgccggcgcg acggttcgct cggggccgaa tcgtcgtcgc agctttcagc
     1381 cggggactcg acccttgatg gcgttggccc aggcgcggta ggtcaaccgg ccctgtgcat
     1441 cgacagaagg caggcgccgc cgcaggccgt cgcgcaggtg cgcacgctcg tcttctccca
     1501 tctgggcgac gcgtgccgcg acccgtggcc cggtctgagc gatctcccag aagcgctcga
     1561 agctttcgaa gctgcgctgc acctcgatcg tccgcgtctc cacgttcacc agcccggcgt
     1621 cttgccagag cgagcgcatc gtctccaggc gcgacgcgtc ctcgctcggt ggcgcgggca
     1681 gcgcgatgcc catcgccttg atctcctcca tcatcgccgc gtagggcaga ccgccgccga
     1741 gcacgtccca ggcgtaggcg gagacgctgc cgccgggtcg ggtgacacgg accatctccg
     1801 ccaccgcctt cgccggatcg ggcacgaaga agatcaccag cgccatcacc gcggcgtcga
     1861 agtgggcgtc ggcccagggc agggcctgcg cgtcgccgac ctggaaggcc gccggcgcgc
     1921 ccgcgggcaa gcgggttcgg gcaaatgcga tctgcgcttc ggcggggtcg atgccctgca
     1981 cctcggccgg ggcgcagcgc tcgaccagca tctcggtgaa ggcagcgttg ccgcagccca
     2041 cgtcgagcca gcgccatccc ggtgcgggag cgagccagcg caagaaggcg tcgccgacgg
     2101 actggctcca gatgcccatg taggtctcgt aggcggcacc gtcgtcaaag cggatcggcg
     2161 ggggattcat gcaggctccg ggagtccatg gggcgactgt agacgccgga gtctgtcggc
     2221 tgcatgccgc gatcagggtg aacttcgatt cgacagcaag acccggagtg cgccgtagga
     2281 tgtgtctgaa gacactgggt gcatgcccac ttcgaagcaa gagatcatga agtccccttc
     2341 atcggacatc acccgagacc ctcgctggac gagcatcgtc acgcgcgaca aggccgccga
     2401 cggcagcttt tggtattccg tcgcgacgac aggggtcttc tgccggccct cgtgcccgtc
     2461 gcgccttgcg aatccgaaga acgttcggct gcatgaaagc ccaggcgcgg ccgaagccgc
     2521 gggctttcgc ccctgccgcc gctgcaaccc ggacggaatc tccatcgacg ttgcaaacgc
     2581 cgccgtcgtc accaaggcct gccggctcat cgacgaggcg gccgagccgc tctcgctgac
     2641 gcagctggcc gagttcgtcg agctcagccc gcactacttc caccgcctct tcaagaaagc
     2701 catggggctc acgccgaaag cctacgcggc ggccgcccgt tcggcccgac tgcgccgggg
     2761 gttggcaacg gctgcgacgg tcaccgaggc gtttcacgag gcgggcttcg ggtcgaacgg
     2821 ccggttctac gaggcctccg cgggcgtgct gggcatgagc ccgacccact accggtccgg
     2881 cggtgtgcag gaagtgctga ccttcgccgt cggccaatgc acgctcggcg ccatcctcgt
     2941 cgcgtcgagc gccaagggcg tcgccgccat cctgctgggg gacgatccgg aggcgttgct
     3001 gcgcgatctg caggatcggt ttccgaaggc ccgactggtg ggcggcgacg cggcctacga
     3061 gcggctggtg gccgaggtgg tgggaagggt cgaagagccc gcgcgcggct tcgacctgcc
     3121 gctcgacgtt cgcggcacgg cgttccagca gcgcgtctgg caggcgctgc gcgcgatccc
     3181 gccgggcgcc acggaaacct actcgagcat cgcgctgaag atcggcgcgg cccgatccgt
     3241 gcgggccgtc gccggcgcct gcgcggccaa cctccacgcc gtggcgattc cgtgccaccg
     3301 cgtcgtgcgg caggacggcg acctctcggg ctaccgctgg ggcgtggagc gcaagcgcga
     3361 gctgatcgag cgagaatccc gggcgcagga gcgggccgcg tgaagcgcga cgactggatc
     3421 gaagccgacg tcgccgcgcg cgtgcaacgc atcgacaccg ccgcactgga gagcgatctc
     3481 gaagcgcagg gctgggcggt gctgcacgac ctgctcgatg ccacggagtg cgacgacatc
     3541 gctgccctct acgccaatgg tgcaggcttt cgtagtcagg tcgtcatggc gcggcatgga
     3601 ttcggccagg gcgaataccg ctacttctcc tatcccctgc cgccgttggt gcacggcttg
     3661 cgaacggcgc tgtatccgct gctcgcgccc ctcgccaatc gatggcacga gcggatgggg
     3721 atggacgccc gcttccccga aaggcacgac gagttcctcg cgcactgcgc cgcttccggc
     3781 cagtcgcgcc ccacgccact gctgctgcag tatggcgccg gcgactacaa ctgccttcac
     3841 caggacctgt acggcgagca cgtcttcccc ttgcaggtgg ccgtgctgct ctccgagccg
     3901 gcaaaggact tcagcggcgg cgagttcgtg ctcaccgagc agcggccgcg catgcagacg
     3961 cgcgcggcgg tcgtcccgct gacccagggc gacgccgtcg tcttcgccgt caacagccgt
     4021 ccggtgcgcg gcacgcgcgg ggactatcgc gtgaagatgc gccacggcgt cagcaagatc
     4081 g
//
LOCUS       DASRDG010000727         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U9974
            k141_574074, whole genome shotgun sequence.
ACCESSION   DASRDG010000727 DASRDG010000000
VERSION     DASRDG010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963502
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:27:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,442
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,405
            CDSs (with protein)               :: 2,405
            Genes (RNA)                       :: 15
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 13 of 22
            Pseudo Genes (internal stop)      :: 3 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574074"
                     /isolate="SMAG_U9974"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="pyrC"
                     /locus_tag="VFA35_12190"
     CDS             <1..843
                     /gene="pyrC"
                     /locus_tag="VFA35_12190"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZT56978.1"
                     /translation="AFEPLMTLYLTDNLPPDEIRRAKAAGIVAVKLYPAGATTNSDAG
                     VTDIRKTYKTLEAMQREGILLLVHGEVTDPSIDIFDREKAFIDTKLISLRRDFPELKI
                     VFEHITTREAAQYVAEADRFVGATVTAHHLLYNRNAIFMGGIRPHYYCLPVLKREEHR
                     RALVAAVASGSAKFFLGTDSAPHATHLKEHAVGCAGCYTALSALELYAEAFDAAGALD
                     RFEAFASFNGPAFYGLPRNAGTVTLRREKWTLPESLPFGETTLKPLRGGESLAWQLAE
                     SAHE"
     gene            836..1609
                     /locus_tag="VFA35_12195"
     CDS             836..1609
                     /locus_tag="VFA35_12195"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZT56979.1"
                     /translation="MNDDLRVALLIDADNLSGDVIEQGIEHLLKLYGAIHFRRAYSSP
                     QKVIEHTELFKRYAIRPMVNVPTGKNSTDIALAVDAIDLAISERPDVVVIGSSDSDYA
                     PLAQRLREKGCRVLGIGQAGKTGGEAPLAYDEFIDLAHRKSPRELPRELPRSTPAPRR
                     PRARAPTVRAKAPVAAPVPAPPPAVPPEVASILAAVPALRNGAPLQLGEVSEALRRND
                     ILTSKRASASGFLKRHAAYFELVPADRPRTVRYRGPKTE"
     gene            complement(1716..2186)
                     /locus_tag="VFA35_12200"
     CDS             complement(1716..2186)
                     /locus_tag="VFA35_12200"
                     /inference="COORDINATES: protein motif:HMM:NF025045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HZT56980.1"
                     /translation="MSLRVRWLRGGALTLVLKQEIDMTMKNALTVVAAVAAAMFAQAT
                     FAQASAPASRAEVKAETKAAEKAGKLTPAGEGSAPVEKSTAKSTKTKEERKAETKAAA
                     KAKKLTPAGEGAAPAAAPSTGPTAARADVKAETKAAVKAGATIKAGEGPDAPKK"
     gene            2375..2751
                     /gene="rnpB"
                     /locus_tag="VFA35_12205"
     ncRNA           2375..2751
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFA35_12205"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2806..>3801
                     /locus_tag="VFA35_12210"
     CDS             2806..>3801
                     /locus_tag="VFA35_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HZT56981.1"
                     /translation="MNPDAHTLWRAPRWALAVLLACLGMLGPFSIDTYLPAFTGIAKA
                     IGATPVEMQQTLSAYLFGFAVMNLFHGALSDSFGRRPVVLVGLVVFTLASVGCALSQH
                     IGALVFFRAVQGMSAGAGIVVSRAVIRDMFPPADAQRVMSQVTIYFGVAPAIAPMVGG
                     FLFVHADWHAIFWFLTAVGVILWIANYQLLPETLHLSQRQSFKLRNLMHGYLEMGGNA
                     RFMMLALASGIPFNGMFLYVLSAPVFLGDHLHLAPGQFFWFFMITIGGIMGGAWLSGR
                     LAGRIKPQHQIRHGFLIMLLVSIANLVLNLLFAPQAWWAIIPLGIYAFGWALMVPV"
BASE COUNT          579 a         1310 c         1276 g          636 t
ORIGIN      
        1 gcgttcgagc cgctgatgac gctctacctc accgacaacc tgccgcccga tgagatcagg
       61 cgtgcgaagg cggcgggcat cgtcgcggtg aagctttacc cagcgggcgc gacgaccaac
      121 agcgacgcgg gcgtgaccga catccgcaag acctacaaga ccctggaagc catgcagcgc
      181 gaaggcatcc tgctgctggt gcacggcgaa gtgaccgatc cgtccatcga catcttcgac
      241 cgcgagaagg ccttcatcga caccaagctg atctcgctgc ggcgcgactt ccccgaactg
      301 aagatcgtgt tcgagcacat cacgacccgc gaggcagcgc agtatgtggc cgaggccgac
      361 cgcttcgtcg gcgcgaccgt caccgcgcac cacctgctct acaaccgcaa cgcgatcttc
      421 atgggcggca tccggccgca ctactactgc ctgccggtgc tcaagcgcga agagcatcgc
      481 cgcgcgctgg tcgcggcagt cgcctcgggc agcgccaagt tcttcctcgg caccgacagc
      541 gcaccgcacg ccacgcacct gaaggaacat gcggtcggct gcgccggctg ctacacagcg
      601 ctgtcggcac tcgagcttta cgccgaggcc ttcgatgccg cgggtgcgct cgatcgcttc
      661 gaggcgttcg cgagcttcaa cggcccggcg ttctacggtt tgccgcgcaa cgccggcacc
      721 gtcacgctgc gccgcgagaa gtggacgctg cccgaatcgc tgccgttcgg cgagaccacc
      781 ctcaaaccgc tgcgcggcgg cgaaagcctg gcgtggcaac tggccgaaag cgctcatgaa
      841 tgacgacctg cgcgtggcgc tgctgatcga cgccgacaac ctctcgggtg acgtgatcga
      901 gcaggggatc gagcacctgc tgaagctgta cggtgcgatc cacttccggc gtgcctattc
      961 gagtccgcag aaggtgatcg agcacaccga gctgttcaag cgctacgcga tccggccgat
     1021 ggtcaacgtg ccgaccggca agaactcgac cgacattgcg ctcgcggtcg acgcgatcga
     1081 cctggcgatc agcgagcgcc ccgacgtggt cgtgatcggc tcgagcgact ccgactacgc
     1141 gccgctcgcg cagcgcctgc gcgagaaggg ttgccgcgtg ctcggcatcg gccaggccgg
     1201 caagaccggc ggcgaggcgc cgctcgcgta cgacgagttc atcgacctcg cgcaccgcaa
     1261 atcgccgcgt gagttgccgc gcgaattgcc gcggtcgaca cccgcgccgc gccggccgcg
     1321 ggcgcgcgcc ccgacggtgc gcgcgaaagc acctgtggcc gcacctgtcc cggcgccgcc
     1381 gcccgcggtg ccgccggagg tcgcgagtat cctggcggcg gtgcccgcct tgcgcaacgg
     1441 cgcgccgctg cagctcggcg aggtcagcga ggcgctgcgg cgcaacgaca tcctgacgag
     1501 caagcgcgcc tcggcgagcg gattcctgaa acgccacgcg gcgtatttcg aactcgtgcc
     1561 cgccgaccgg ccgcgcaccg tgcgctatcg gggtccgaag acagagtgaa cagcgcggcg
     1621 cggcgcgttc gtgtcccgcg cggcgcaaaa acaaacagcg gcccgagggg ccgctgtctt
     1681 gaaccacctg gcgcgcgaag cgccgggccg gttgcttact tcttcggagc gtccgggcct
     1741 tcgccggcct tgatcgtcgc gccggccttg acggcggcct tggtttcggc cttgacgtcg
     1801 gcgcgcgcag cggtcgggcc ggtcgacggc gcagctgccg gggcagcgcc ctcgccagcc
     1861 ggcgtcagct tcttggcctt ggcggcggcc ttggtttcgg ccttgcgctc ttccttggtc
     1921 ttggtcgact tggcggtcga cttttccacc ggagccgagc cttcgcctgc cggcgtgagc
     1981 ttgccggcct tttcggcggc cttggtttcg gccttgactt cggcgcgcga agccggagcc
     2041 gacgcttggg cgaacgtggc ttgggcgaac atggcggcgg cgacagcggc cacgacggtc
     2101 agagcgtttt tcatcgtcat atcaatctcc tgttttagaa cgagggtaag ggcaccaccg
     2161 cggagccaac gcactcggag cgacatgccc tcatcaagtc ggcttgctgc cgagctgcga
     2221 aacgcagacc ggcggagcgc ggcacgtatt agaacgcggg ttggcgccat cggttgacca
     2281 agaccctcat tcgacggccg gcgggcgccg cgaacccccg atttgttgcg ccggtgccgc
     2341 acccagcccg atcggcggcc ataatcgcgc ccgtgaagtg ggccagaccg ccgctggtgc
     2401 aaggcttgag agggcgcact ggaggaaggt ccggactgcg cagggcggcg tagcagctaa
     2461 cggctgtcca ccgcgaggtg aggatcagag caacagagac gagtcgatcg gggccgcaag
     2521 gcatcggtcg ggtgaaacgg gcaatctcta cgcgcagcaa caccaaatag gcacacattg
     2581 agcggcgcgg cagcgaccgc gttgttcagg gccctggctc cggggactgt gtgcgggtag
     2641 gtggcatcga gccgtcgcag cgatgcacgg cccagaggaa tggcggtcac ggccggcgct
     2701 gcgcaagcgg cgccggctgc acagaatccg gcctatcggc ccgcttcaca cttttctcga
     2761 accggccggt cggcaccggt gcacgccgta gactctggcg ctccgatgaa tcccgacgca
     2821 cacaccctct ggcgcgcccc gcgctgggcc ctggccgttc tgctcgcgtg cctgggcatg
     2881 ctcgggccgt tctcgatcga cacctacctg ccggcgttca ccggcatcgc gaaggccatc
     2941 ggcgccacgc cggtcgagat gcagcagacg ctctcggcct atctgttcgg ctttgcggtc
     3001 atgaacctgt tccacggcgc gctgtccgac agcttcggcc ggcgcccggt cgtcctcgtc
     3061 ggcctggtcg tcttcactct cgcatcggtc ggctgcgcgc tctcgcagca catcggcgcg
     3121 ctggtcttct ttcgcgccgt gcagggaatg tcggcggggg cggggatcgt cgtctcgcgc
     3181 gcggtgatcc gcgacatgtt cccgcccgcc gacgcgcagc gcgtgatgtc gcaggtgacg
     3241 atctacttcg gcgtggcgcc ggcgatcgcg ccgatggtcg gcggtttcct gttcgtgcac
     3301 gccgactggc acgcgatctt ctggtttctg accgccgtgg gcgtgatcct gtggatcgcg
     3361 aactaccagt tgctgcccga gacgctgcac ctgagccagc gccagtcgtt caagctgcgc
     3421 aacctgatgc acggctacct cgagatgggc ggcaacgcgc gcttcatgat gctggcgctg
     3481 gccagcggca tcccgttcaa cggcatgttc ctgtacgtgc tgtcggcgcc ggtgttcctc
     3541 ggcgaccacc tgcacctcgc gccggggcag ttcttctggt tcttcatgat cacgatcggc
     3601 ggcatcatgg gcggtgcgtg gctctccggg cggctggccg ggcgcatcaa gccgcagcac
     3661 cagatccgcc acggcttcct gatcatgctg ctggtgtcca ttgccaacct cgtgctgaac
     3721 ctgctgttcg cgccgcaagc ctggtgggcg atcattccgc tgggcatcta cgcattcggc
     3781 tgggcgctga tggtgccggt c
//
